LOCUS       DAGCXG010000001        96309 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGCXG010000001 DAGCXG010000000
VERSION     DAGCXG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 96309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.001"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(594..1985)
                     /locus_tag="MB022_000001"
     CDS             complement(594..1985)
                     /locus_tag="MB022_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBT4465034.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="MB022_000002"
     CDS             complement(2198..2584)
                     /locus_tag="MB022_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBT4465035.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..3263)
                     /locus_tag="MB022_000003"
     CDS             complement(2640..3263)
                     /locus_tag="MB022_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBT4465036.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3327..3995)
                     /locus_tag="MB022_000004"
     CDS             complement(3327..3995)
                     /locus_tag="MB022_000004"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4465037.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4007..4447)
                     /locus_tag="MB022_000005"
     CDS             complement(4007..4447)
                     /locus_tag="MB022_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465038.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4506..5147)
                     /locus_tag="MB022_000006"
     CDS             complement(4506..5147)
                     /locus_tag="MB022_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465039.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            complement(5173..6132)
                     /locus_tag="MB022_000007"
     CDS             complement(5173..6132)
                     /locus_tag="MB022_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4465040.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(6196..7371)
                     /locus_tag="MB022_000008"
     CDS             complement(6196..7371)
                     /locus_tag="MB022_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4465041.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNKSVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFADCKIKLDN"
     gene            7878..9578
                     /locus_tag="MB022_000009"
     CDS             7878..9578
                     /locus_tag="MB022_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465042.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(9680..10513)
                     /locus_tag="MB022_000010"
     CDS             complement(9680..10513)
                     /locus_tag="MB022_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBT4465043.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10817..12982)
                     /locus_tag="MB022_000011"
     CDS             complement(10817..12982)
                     /locus_tag="MB022_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4465044.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            13242..15536
                     /locus_tag="MB022_000012"
     CDS             13242..15536
                     /locus_tag="MB022_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4465045.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            15529..16194
                     /gene="pgmB"
                     /locus_tag="MB022_000013"
     CDS             15529..16194
                     /gene="pgmB"
                     /locus_tag="MB022_000013"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4465046.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            16187..17194
                     /locus_tag="MB022_000014"
     CDS             16187..17194
                     /locus_tag="MB022_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4465047.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            17216..18235
                     /locus_tag="MB022_000015"
     CDS             17216..18235
                     /locus_tag="MB022_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4465048.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(18516..19883)
                     /gene="nhaC"
                     /locus_tag="MB022_000016"
     CDS             complement(18516..19883)
                     /gene="nhaC"
                     /locus_tag="MB022_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4465049.1"
                     /translation="MNKNISLKESIVVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(19993..21414)
                     /locus_tag="MB022_000017"
     CDS             complement(19993..21414)
                     /locus_tag="MB022_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4465050.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(21657..23534)
                     /gene="tdc"
                     /locus_tag="MB022_000018"
     CDS             complement(21657..23534)
                     /gene="tdc"
                     /locus_tag="MB022_000018"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBT4465051.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(23825..25081)
                     /locus_tag="MB022_000019"
     CDS             complement(23825..25081)
                     /locus_tag="MB022_000019"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBT4465052.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(25193..25398)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25661..26962
                     /locus_tag="MB022_000020"
     CDS             25661..26962
                     /locus_tag="MB022_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4465053.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(27031..27630)
                     /locus_tag="MB022_000021"
     CDS             complement(27031..27630)
                     /locus_tag="MB022_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4465054.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            27884..28765
                     /locus_tag="MB022_000022"
     CDS             27884..28765
                     /locus_tag="MB022_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465055.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            28778..30388
                     /locus_tag="MB022_000023"
     CDS             28778..30388
                     /locus_tag="MB022_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBT4465056.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(30465..31124)
                     /locus_tag="MB022_000024"
     CDS             complement(30465..31124)
                     /locus_tag="MB022_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4465057.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(31407..32072)
                     /locus_tag="MB022_000025"
     CDS             complement(31407..32072)
                     /locus_tag="MB022_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBT4465058.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(32219..32824)
                     /gene="dhaL"
                     /locus_tag="MB022_000026"
     CDS             complement(32219..32824)
                     /gene="dhaL"
                     /locus_tag="MB022_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBT4465059.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(32852..33862)
                     /gene="dhaK"
                     /locus_tag="MB022_000027"
     CDS             complement(32852..33862)
                     /gene="dhaK"
                     /locus_tag="MB022_000027"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBT4465060.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(33837..34283)
                     /gene="dhaM"
                     /locus_tag="MB022_000028"
     CDS             complement(33837..34283)
                     /gene="dhaM"
                     /locus_tag="MB022_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBT4465061.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            34386..34706
                     /locus_tag="MB022_000029"
     CDS             34386..34706
                     /locus_tag="MB022_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBT4465062.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(34818..35687)
                     /locus_tag="MB022_000030"
     CDS             complement(34818..35687)
                     /locus_tag="MB022_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4465063.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(35783..37087)
                     /locus_tag="MB022_000031"
     CDS             complement(35783..37087)
                     /locus_tag="MB022_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4465064.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKRKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRQRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(37084..37245)
                     /locus_tag="MB022_000032"
     CDS             complement(37084..37245)
                     /locus_tag="MB022_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465065.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(37249..37592)
                     /locus_tag="MB022_000033"
                     /pseudo
     CDS             complement(37249..37592)
                     /locus_tag="MB022_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37767..39902)
                     /locus_tag="MB022_000034"
     CDS             complement(37767..39902)
                     /locus_tag="MB022_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4465066.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(39985..41640)
                     /locus_tag="MB022_000035"
     CDS             complement(39985..41640)
                     /locus_tag="MB022_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4465067.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(41642..41956)
                     /locus_tag="MB022_000036"
     CDS             complement(41642..41956)
                     /locus_tag="MB022_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4465068.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            42126..42386
                     /locus_tag="MB022_000037"
     CDS             42126..42386
                     /locus_tag="MB022_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBT4465069.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            42400..42714
                     /locus_tag="MB022_000038"
     CDS             42400..42714
                     /locus_tag="MB022_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBT4465070.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(43444..43998)
                     /locus_tag="MB022_000039"
     CDS             complement(43444..43998)
                     /locus_tag="MB022_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBT4465071.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(44124..46052)
                     /locus_tag="MB022_000040"
     CDS             complement(44124..46052)
                     /locus_tag="MB022_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4465072.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(46358..47074)
                     /locus_tag="MB022_000041"
     CDS             complement(46358..47074)
                     /locus_tag="MB022_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4465073.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(47075..48898)
                     /gene="glpO"
                     /locus_tag="MB022_000042"
     CDS             complement(47075..48898)
                     /gene="glpO"
                     /locus_tag="MB022_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBT4465074.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(48912..50408)
                     /gene="glpK"
                     /locus_tag="MB022_000043"
     CDS             complement(48912..50408)
                     /gene="glpK"
                     /locus_tag="MB022_000043"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBT4465075.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(50544..52004)
                     /locus_tag="MB022_000044"
     CDS             complement(50544..52004)
                     /locus_tag="MB022_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465076.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(51994..53289)
                     /locus_tag="MB022_000045"
     CDS             complement(51994..53289)
                     /locus_tag="MB022_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4465077.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(53621..54298)
                     /locus_tag="MB022_000046"
     CDS             complement(53621..54298)
                     /locus_tag="MB022_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBT4465078.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(54328..55419)
                     /locus_tag="MB022_000047"
     CDS             complement(54328..55419)
                     /locus_tag="MB022_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBT4465079.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(55520..55954)
                     /locus_tag="MB022_000048"
     CDS             complement(55520..55954)
                     /locus_tag="MB022_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4465080.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(56538..57995)
                     /gene="ascB"
                     /locus_tag="MB022_000049"
     CDS             complement(56538..57995)
                     /gene="ascB"
                     /locus_tag="MB022_000049"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4465081.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(57992..59887)
                     /locus_tag="MB022_000050"
     CDS             complement(57992..59887)
                     /locus_tag="MB022_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4465082.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGAVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(60003..61691)
                     /locus_tag="MB022_000051"
     CDS             complement(60003..61691)
                     /locus_tag="MB022_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBT4465083.1"
                     /translation="MDLNKRQLQILVVLRNKNHWITSEELAEALGTNKKTIQTEIKNM
                     LAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRASTIVTYLLFQ
                     KGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLKIIAEENSKRI
                     FLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSGEDFIRFSRYL
                     VLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIENRLLELNYLM
                     INDEKNKEIEKNLRAFEQTIILFLDLPVSLLFHETEFLLTHIKQMKTRMDAGHNTMNH
                     FVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFRKQGEILIVSN
                     QPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTTEQELIFEWDD
                     LIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKIHKKIDSIQQL
                     ISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKRVFFCLL"
     gene            complement(61771..63267)
                     /locus_tag="MB022_000052"
     CDS             complement(61771..63267)
                     /locus_tag="MB022_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4465084.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(63461..65083)
                     /locus_tag="MB022_000053"
     CDS             complement(63461..65083)
                     /locus_tag="MB022_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBT4465085.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(65532..66845)
                     /locus_tag="MB022_000054"
     CDS             complement(65532..66845)
                     /locus_tag="MB022_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBT4465086.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(67090..67314)
                     /locus_tag="MB022_000055"
     CDS             complement(67090..67314)
                     /locus_tag="MB022_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465087.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(67334..67756)
                     /locus_tag="MB022_000056"
     CDS             complement(67334..67756)
                     /locus_tag="MB022_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBT4465088.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(67899..68609)
                     /locus_tag="MB022_000057"
     CDS             complement(67899..68609)
                     /locus_tag="MB022_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465089.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(68627..69124)
                     /locus_tag="MB022_000058"
     CDS             complement(68627..69124)
                     /locus_tag="MB022_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4465090.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(69161..69613)
                     /locus_tag="MB022_000059"
     CDS             complement(69161..69613)
                     /locus_tag="MB022_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4465091.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(69748..70353)
                     /locus_tag="MB022_000060"
     CDS             complement(69748..70353)
                     /locus_tag="MB022_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4465092.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(70803..71423)
                     /locus_tag="MB022_000061"
     CDS             complement(70803..71423)
                     /locus_tag="MB022_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBT4465093.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(71425..72294)
                     /locus_tag="MB022_000062"
     CDS             complement(71425..72294)
                     /locus_tag="MB022_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBT4465094.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIYVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(72317..72724)
                     /locus_tag="MB022_000063"
     CDS             complement(72317..72724)
                     /locus_tag="MB022_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBT4465095.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(72743..74032)
                     /locus_tag="MB022_000064"
     CDS             complement(72743..74032)
                     /locus_tag="MB022_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4465096.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(74059..74361)
                     /locus_tag="MB022_000065"
     CDS             complement(74059..74361)
                     /locus_tag="MB022_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4465097.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(74386..76479)
                     /locus_tag="MB022_000066"
     CDS             complement(74386..76479)
                     /locus_tag="MB022_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4465098.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(76968..77732)
                     /locus_tag="MB022_000067"
     CDS             complement(76968..77732)
                     /locus_tag="MB022_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBT4465099.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(77843..78712)
                     /gene="mdcB"
                     /locus_tag="MB022_000068"
     CDS             complement(77843..78712)
                     /gene="mdcB"
                     /locus_tag="MB022_000068"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBT4465100.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(78737..79813)
                     /locus_tag="MB022_000069"
     CDS             complement(78737..79813)
                     /locus_tag="MB022_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBT4465101.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(79837..80430)
                     /locus_tag="MB022_000070"
     CDS             complement(79837..80430)
                     /locus_tag="MB022_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBT4465102.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(80434..82110)
                     /gene="mdcD"
                     /locus_tag="MB022_000071"
     CDS             complement(80434..82110)
                     /gene="mdcD"
                     /locus_tag="MB022_000071"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBT4465103.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(82100..82399)
                     /locus_tag="MB022_000072"
     CDS             complement(82100..82399)
                     /locus_tag="MB022_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBT4465104.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(82501..84147)
                     /gene="mdcA"
                     /locus_tag="MB022_000073"
     CDS             complement(82501..84147)
                     /gene="mdcA"
                     /locus_tag="MB022_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBT4465105.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(84154..85065)
                     /locus_tag="MB022_000074"
     CDS             complement(84154..85065)
                     /locus_tag="MB022_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBT4465106.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            85185..86042
                     /locus_tag="MB022_000075"
     CDS             85185..86042
                     /locus_tag="MB022_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4465107.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(86229..86591)
                     /locus_tag="MB022_000076"
     CDS             complement(86229..86591)
                     /locus_tag="MB022_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBT4465108.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            86937..88376
                     /locus_tag="MB022_000077"
     CDS             86937..88376
                     /locus_tag="MB022_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465109.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(88383..90935)
                     /locus_tag="MB022_000078"
     CDS             complement(88383..90935)
                     /locus_tag="MB022_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBT4465110.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(91049..92380)
                     /locus_tag="MB022_000079"
     CDS             complement(91049..92380)
                     /locus_tag="MB022_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4465111.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVIMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(92346..93824)
                     /locus_tag="MB022_000080"
     CDS             complement(92346..93824)
                     /locus_tag="MB022_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4465112.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(94129..95964)
                     /gene="tdc"
                     /locus_tag="MB022_000081"
     CDS             complement(94129..95964)
                     /gene="tdc"
                     /locus_tag="MB022_000081"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBT4465113.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        27828 a        20393 c        16612 g        31476 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaattttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     3121 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     3181 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     3241 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     3301 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     3361 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3421 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3481 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3541 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3601 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3661 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3721 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3781 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3841 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3901 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3961 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     4021 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     4081 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     4141 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     4201 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     4261 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     4321 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     4381 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4441 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4501 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4561 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4621 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4681 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4741 gtcgttttga tcagattccg ttttttactg atcactttta aaaagctttt tagaatcttt
     4801 tgctgacgtc taaaaataga aaaagaattt tcgtcatcta tttgataacg catttgtttt
     4861 tgcagttctg ctgcagtcag atggatcttc ccatcgtttg tatcgatcat taaaccttct
     4921 ggaaacagtt caaccactgc tcgatcccaa gattcaagag aaagctcgat ataatctttt
     4981 actggtaatt ccaattctac cttaaaaaag tgcaaaacag ccgacagccc ttctgcttga
     5041 tagatttctc ctaatgttag cccctctaga tggaactctg gtgaaaaatg cagcaatgtt
     5101 ttattttcct gcaattgaac atatagaaat gtagatcgct caatcagagt atcttttaaa
     5161 acatttcgtt tgtcaaatta agttagacaa atcatctttt gtgacgtaac tcaaaaatac
     5221 ttccattggt gtccgatagt gtaatgattt tctaggaata tgatttcgtt tcgatgccac
     5281 cgattggata aaggtttcag atacaccatt aaaatccata gctttataca aaccatcttt
     5341 acgtaataag ccatttgaat gttcattcaa tcctcgttga gaaggtgttc ctggatccgc
     5401 aaaataaatt gaaatatcgt tggtattact aatctttttc caatttgaga attcttttcc
     5461 acaatcaaat gtgatggatt taaataagtt ttttgggaca caacgaaacc attgattcag
     5521 cctattttca atgtctttag cctgtctgcc ttcgggtttc aaggtaatga tgactttaga
     5581 tagccgttca actaatgtga tgaccgcgct tttatgtttt ccacccacga tagtgtcacc
     5641 ttccaggtga ccaaattcat gatcaaactg ggtatagtct gtctttctgt ctcggattga
     5701 ccggcgaaac ccttgttttc ctcttttttc tttatgaccg tttggttttc tttttccctt
     5761 cattggtaaa tcgtgagtgt caaaggtttt atctttaaac attcgataca gtgtgcgtac
     5821 cgaacaagca ataggaaact ctgctcgacc aataaggaca tcaggagccc acccttgaac
     5881 aacctttttt tgaatgtatt cctgttgctc atcaggtaaa acaatcggat gtcttccgca
     5941 gttacgtttg tttttcttat attgtttata gtagctcatt gcgcttccac cagatttaag
     6001 aaaacgatag accttataaa tagtttgtcg agaacgttgt aacagattgg ctacttttga
     6061 aacaggttgt gattgattga aataagcttc tatcattacg agttcagttg gtgtaagatg
     6121 gttataggtc atttgtactc actccttata attctttcgt cggaactatt ttgagtgtag
     6181 cacaaatgac tttttttagt tgtctagctt aattttacaa tcggcgaaat aaaaaccatt
     6241 tttttgaagc tcttttacaa gttttcgccc ctcaaaaaag tatatcttct cttgttttac
     6301 ttttctaata ccaaaagaaa cagcaacttt aggatcatca gctgaaacga ttcgataagt
     6361 tccaccaggc aattgatcca gcttgacttt cccatgatgg tcgctttgta ctgaaaacgg
     6421 cacaccattt ctagtaagtg tttttttctt atgtttcaaa atgaatgaac taccatttac
     6481 cggtttccct gacgaatcac taataataaa tgaaaaatca agcacttttt tacttaatct
     6541 atactgatag attagtcgtc cgataaacac taacagtatg actacagcta ttatcgttcc
     6601 tgcaagaata aaccattgcc gcatggttct ttcctgattt ccttttcctt cttctttttt
     6661 taccgatttg ttataaggaa tccgatgtcc tgtcactagc aaacgatgcg tgttgatcat
     6721 atacggcgta catgtaagca aggtaactaa gtcttctcct tctgaaatcg ttagaacaga
     6781 cgtgtcatct ggcagtacca cttgtatatt tttaacttga taagccaatt tctcccccaa
     6841 aacagtcaaa ataaacgtgt ctccgttttc taaacgatct aattcggtaa ataaccgtcg
     6901 attaggtaaa ccgctgtgag cagagatcac tgaatgcgtc gaatttccac caagcgggaa
     6961 agaggttcct tgcaagacgc ctgctccatt ttcaaggatc ctaccattga gccgatcata
     7021 aagtgaaata tttaattgga tcttaggtat attgatactt ccgatcagcc attcctctaa
     7081 ttggctggat gttgtacttt catttctgtt cttttcattg aaaggatcag taccaggatt
     7141 gaatccttgg tcagttaatc gttggttttt ctcctccatt ttctttcgct gcacttcaat
     7201 ttcagctgcg gtccgttttt gtacttgttc gatacgtttt tgatcaataa aactatttaa
     7261 agaatccaca taaaatggat ataaagcgat cagtaaccct acgacgatac atattaataa
     7321 aaacaaacga aacaaaagtc gacgagactg tttttgctta ctctgcttca catgtgtcct
     7381 cccttgcaag tttttttgtt ctttctttta ttatatgggt tcaggaaaaa aatagaaaat
     7441 gaaagccgat gaggttgtga aaaagctgtc ctgcatcaag taataagtac agcccaaaaa
     7501 aatagctact catatttttt gttgggttgg gcttattacc gcagatgcaa gcttttgaac
     7561 accgtttaag gaagaggttg tgaaaactgc gttatgcttt gagtaataag agcgctagtc
     7621 agaaaacagc ttctcatatt ttctaggcta gtgagcttat taccgagaag cagctgattg
     7681 aacaccgttt accgagaggt catgaaaaag ctactttgtt tcgagtatta agaaaaattc
     7741 tgtttcaaaa caaggagatg caacaaaact tatgtcgcat cctctctcct tagaactttc
     7801 acttatgttt ttcttgtaaa attcataaat attttcatga attttctaat ttcttgccta
     7861 cctctcgcat aggatatatg atgaatttat ctaaagtttg caaaggagtt cgacaattga
     7921 aaaaatatgg cccattttta gcaatttttt tcattgcttt ggcatttctc atgcctcatt
     7981 tattcacaca taaactaatt ttgggtgctg atgccccatt ccattacaat cggttctatg
     8041 atgccgcgca acaaattaaa tatggggatt tccattattt tccttccctt tatggattcc
     8101 agcaaagtgg caggatcgtg aatgccgttt acggcccatt tttcgcttat ttacaaggat
     8161 tgatcgtgtt gcttgcaggt agctggtttc attatcagat tctttcaaat ttcattcttt
     8221 atcttatttc aggattttct atgctgattt tactgagata tgtcaatgtt cgaaattggg
     8281 taagtgtacc agtagccgtt ttattcatga gtacgtattc tattcaatac tggtctatca
     8341 accaaggatt cactagctgg gggacttgtt tttatccttt atgtatgatc cctattgctg
     8401 atttcgtcct tcataaaaag ttccctgtta tcaaagtcgc tgtctcagtg ggacttatga
     8461 cacagatcca tttactgtct tcgatcatgt tagctttgat gtatatccct ttttatcttt
     8521 attacttttt taaccagact gaaaaaggaa aagcactgct tcaactgatt aagtcggtct
     8581 tgctttattt tgttctgaca gcaaatattt gggcaggatt agctattgtt tacagtggga
     8641 ataagattct tcctcccttt gttaacagag aaatgagtca aaaggcgata gatctggatg
     8701 gcagttattg gctccattac cctaaggcct ttcctatatt gatattatca ggttttttgc
     8761 tttttatttt ctttcaacaa aagaacacct tattccaaaa aatcgtgttg tacacaagtc
     8821 ttgttttctt ttttctttct actagcttga ttccatggac gacattgatc gaaaaaaatg
     8881 tgccttttat tagtttgatc cagtttccat ttcgattttt cgtgccattt acgctattgc
     8941 ttctacttta ccttgccctt tcactgaacc agtggtctga acaaaagtgg ttccgtcttt
     9001 taagtatatt tggcattctt attttttcag cacagacgat ccagaactta taccagcatc
     9061 tggaaaagtg ggaaaatgaa acatttgtca gtcgacatac ttatctattc gatacgccgg
     9121 aagaagcaag gaaaacattc ttcagcaaag atatgtctac cagcttgttc gttttccaaa
     9181 aaaccacacc tgactatctg ccaatctata aggagacaaa agacaataaa tacgaccggt
     9241 ataacgaaga aatactagaa aacgaaacaa cttttataaa aacacatcca ggcggtcttt
     9301 tgacaatcaa gtggaaaaac actggggaca aaactgttca tatccctgtt atcgtctacg
     9361 atcggactgt gctacagcaa aatggaaaaa cattgactga ttatgaagta acagatatcg
     9421 ggaccccaat cgtgaagcag aaaaaaggaa tcaacgagct cacgcttcat tatcaaacgc
     9481 caatctattt ctatttcatt ctttctctta ctttgattgg ctggtttacc ttgctatgtt
     9541 tatttattta tcatagatac aagcttttac gtgcttaggc aaaagggata aatacatccg
     9601 atcaaaaaac tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt
     9661 atgttatatg ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat
     9721 cactgatgat tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc
     9781 cttcagggac gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta
     9841 ttttcttttc cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa
     9901 gaagaaggca actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat
     9961 cacctaggaa aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact
    10021 caaaataaaa gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa
    10081 tgtcctgatc atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt
    10141 tcttttgaga ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg
    10201 catttccttc ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact
    10261 cttttccgtg ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag
    10321 gtacttgatt ctttacctga cagaacttca ttagcttcgc aggttctact tcttcagatt
    10381 ccagtaattg gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat
    10441 ctgctaattc ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg
    10501 tcatgaattt cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa
    10561 tttttgtcac aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc
    10621 aatggaccag gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt
    10681 tcacgacctc tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa
    10741 acgaaaaata ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct
    10801 ttttcacaac ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac
    10861 ctaattgctt gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg
    10921 gatctttttc ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc
    10981 ctacttcttg gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat
    11041 cgattcccat atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt
    11101 tgctagggaa aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg
    11161 gaataacagc aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag
    11221 ataaaggaat cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt
    11281 gcttactacc tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc
    11341 ctgattgcag cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg
    11401 cttggactcc attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt
    11461 cagtccctac tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga
    11521 catctttgat attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg
    11581 ctgttcctgt tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt
    11641 tacgtcccgg tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa
    11701 caacaccaaa agcgatcaca cacaacacga agttgataag atctccacct agacccgctt
    11761 tgatagctaa cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg
    11821 gcaagatatc tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg
    11881 gcacagcagc aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag
    11941 ccaacgctgc tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac
    12001 gatacatcgc aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt
    12061 tgaaacgagc cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac
    12121 ctttaagatt cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt
    12181 gtgtccccgc ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga
    12241 taggaatcgt cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa
    12301 ataaaaacgg tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat
    12361 tattgattcc agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg
    12421 accataagat aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg
    12481 gcaattttct ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga
    12541 tccctacgaa tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt
    12601 taaccatgat cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt
    12661 cacttgtgac accaaagata gccccagtga tccgattgat taaaataaat gagatcccag
    12721 ctgcaaatgc tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata
    12781 aaatatgaag attaccaata attgcccaac caatattttc aattactccg cccgttgtga
    12841 caagcggcgt ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc
    12901 cagctgccgg cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact
    12961 caaaactaaa tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg
    13021 tattatagcg tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc
    13081 attctataaa acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt
    13141 ttttaagctt ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg
    13201 tttattatga acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac
    13261 tatttgctat tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac
    13321 tgcaagaatc tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag
    13381 aaacttactc aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata
    13441 agacacgtgt cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg
    13501 ccttgaactt tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt
    13561 tggaaccaga agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat
    13621 tcacaatcac tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg
    13681 tcaaaaaaga atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag
    13741 tcaaactcgt atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac
    13801 atttttggaa tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa
    13861 ctattccaaa caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg
    13921 ttaatggaaa atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct
    13981 tgtctgcaga attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta
    14041 ctagtagaga tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc
    14101 agacaaacaa ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga
    14161 agcttgccga tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt
    14221 tgttccaact attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag
    14281 gattcacagg agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc
    14341 ctctttatct tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata
    14401 atcaactgcc gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc
    14461 ctatggtcac cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga
    14521 ttcaccgaaa tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata
    14581 cggaatattt gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag
    14641 accgtgtcca tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa
    14701 acgaatatga gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct
    14761 tgcgttacac gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg
    14821 aagaagaact agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag
    14881 aattaggaat ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg
    14941 atattcccac aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat
    15001 cttgcttcat caagcaggca gatgtactac aggggattta tttcttcaat gatactttta
    15061 caaaagaaga aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt
    15121 ccctttcgcc aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg
    15181 tagaaatgta ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag
    15241 acggcttgca catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc
    15301 aaatgaaaac tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag
    15361 cttattcttt ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac
    15421 aagttacttt cgagttatta gaaggttctc ctctagaaat ttacgtctat gaacagtctt
    15481 attctttagc ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg
    15541 gtattgtttg atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg
    15601 cgaaagcttg gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa
    15661 ggcgtcagcc gagaagactc actctctttg cttttagctt atggcggaaa agaacactct
    15721 ttttcaaagg aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg
    15781 atccagacga tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta
    15841 aaagaagaaa aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt
    15901 gaaaaaatgg gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac
    15961 ggaaaacctg cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa
    16021 gaatgtatcg ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct
    16081 caaccaattg gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat
    16141 acgacttttc ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg ataatccaga
    16201 gatctccgtc gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta
    16261 cttagaagtc gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa
    16321 agaaggaaaa tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga
    16381 caaaagcttt tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg
    16441 gggtgagcat agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg
    16501 ctggtctttc caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt
    16561 cttttactta aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta
    16621 ccgtttaaca aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat
    16681 ctgcaatccg accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta
    16741 tgaacatcag ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc
    16801 tactggaaat tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact
    16861 gcaagagatc cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc
    16921 taggctttcc aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat
    16981 agcaacttct aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc
    17041 tgtcaatgga aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc
    17101 tgatatcgtt catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat
    17161 cagtcagacg atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc
    17221 tattaccgta aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt
    17281 catcaacgat catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga
    17341 tgaactaggt tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc
    17401 catcggcgta atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct
    17461 tgagatcatg gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc
    17521 tactgctaaa aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca
    17581 agtagatggt tttattctag tgtattcaga tagtcaagat cctgtcattg attatttata
    17641 ccaaaaccac atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt
    17701 ttatgtagat aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa
    17761 tggacataag aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg
    17821 atatttcggt tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt
    17881 actagagcat ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac
    17941 agcattggtg gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta
    18001 cggctatgtc attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac
    18061 attaacacac ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc
    18121 tatgcaaaag ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat
    18181 ccctcatcgc ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc
    18241 tgactcacta aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata
    18301 ctacttctaa cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt
    18361 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    18421 gctgaaattc acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt
    18481 taaacgcttc tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc
    18541 aataaaaaac cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa ggcgaatggc
    18601 aagtaatgca acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac
    18661 ggtacgagag aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt
    18721 gataagccaa cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga
    18781 tactgttctc caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa
    18841 cgagaaggtc ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat
    18901 ttgatcagta acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata
    18961 ctcatgatcc cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca
    19021 taaccattca tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa
    19081 gaaagaacga gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg
    19141 attgcccccc caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc
    19201 gctttcatgt tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt
    19261 cctaatatcg tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata
    19321 ccagcgatat gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct
    19381 gataaaggcg agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt
    19441 gcatcattga atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc
    19501 gttgtaaatg aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt
    19561 aaaaagaaac gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca
    19621 gataaaatcc atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc
    19681 cctggagtga ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat
    19741 ctgccataaa acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg
    19801 aatccgatga tcagtactcc taggagtgcc aacaacacga tcaatacaac gatactttct
    19861 tttaaagaaa tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat
    19921 gaaaaaacta ttcacttggc aggacctcct actaaatcag cgacaaaacg tcggctgaaa
    19981 gacttatttt ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt
    20041 cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa
    20101 taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa
    20161 gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg
    20221 acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta
    20281 cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc
    20341 atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac
    20401 atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc
    20461 ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa
    20521 aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag
    20581 gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg
    20641 tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc
    20701 gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga
    20761 actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat
    20821 atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac
    20881 tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata
    20941 tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa
    21001 gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc
    21061 aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac
    21121 catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc
    21181 cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc
    21241 accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat
    21301 caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat
    21361 tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga
    21421 cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac
    21481 gaaacaattc agcgaatatt tgaggggatt ctttgagtct attgcttgaa gggtttcttc
    21541 aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc
    21601 aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat
    21661 tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct
    21721 gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt
    21781 acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct
    21841 agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt
    21901 gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg
    21961 tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag
    22021 tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact
    22081 ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc
    22141 attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg
    22201 gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata
    22261 tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata
    22321 tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc
    22381 gttacagatt ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa
    22441 tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga
    22501 atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct
    22561 acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta
    22621 tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt
    22681 ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga
    22741 tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca
    22801 gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa
    22861 tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct
    22921 aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca
    22981 actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac
    23041 caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta
    23101 tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga
    23161 gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa
    23221 gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga
    23281 acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca
    23341 gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc
    23401 atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag
    23461 atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac
    23521 aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca
    23581 ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa
    23641 tatgtatcaa ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt
    23701 tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc tattttagga
    23761 tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta
    23821 aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa
    23881 acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac
    23941 cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa
    24001 tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg
    24061 gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga
    24121 acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg
    24181 taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt
    24241 tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta
    24301 catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg
    24361 gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca
    24421 tcaatgggat cgttaaaccg aacgcttcta cttctggtcc ttctaatttt cggattaggt
    24481 ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt
    24541 atgttttatg cagatgcaaa aagtcgatag attgcagaat ttggtaagta aactctgtaa
    24601 atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt
    24661 tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt
    24721 agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa
    24781 gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc
    24841 cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg
    24901 ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac
    24961 cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc
    25021 tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca
    25081 ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta
    25141 attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga
    25201 cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat
    25261 ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc
    25321 atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt
    25381 ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg
    25441 tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta
    25501 attccgaaca ttttattttc aattattgtt aaaagaaata aatttttctg tattttttct
    25561 gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa
    25621 tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat
    25681 tattttagga gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt
    25741 catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc
    25801 aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag
    25861 ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga
    25921 aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga
    25981 tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt
    26041 ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg
    26101 ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca
    26161 aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc
    26221 attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc
    26281 tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa
    26341 agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc
    26401 tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt
    26461 aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa
    26521 agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac
    26581 atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt
    26641 gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg
    26701 tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa
    26761 gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt
    26821 aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga
    26881 tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa
    26941 agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca
    27001 ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat
    27061 caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga
    27121 aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc
    27181 cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg
    27241 cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat
    27301 caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat
    27361 ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc
    27421 taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata
    27481 tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc
    27541 agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt
    27601 ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt
    27661 ataatatata tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat
    27721 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
    27781 tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
    27841 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
    27901 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
    27961 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
    28021 gattcggaca cttttaggga ttataaaaaa ggacggcgga acggcaacga tttttgggca
    28081 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
    28141 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
    28201 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
    28261 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
    28321 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
    28381 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
    28441 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg
    28501 aacagtcgta gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
    28561 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
    28621 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
    28681 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
    28741 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
    28801 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
    28861 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
    28921 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
    28981 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
    29041 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
    29101 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
    29161 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
    29221 cagctgtacg atgatttttt tccaagcaga ttcgatcact gcagaaggtg cttggctttt
    29281 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
    29341 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
    29401 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg
    29461 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
    29521 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
    29581 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
    29641 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
    29701 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
    29761 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag
    29821 cacgatcatg gtcgtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg
    29881 tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag
    29941 gaaccaactt tattggacca atatgttact agctattcta tttgctatat tggcaatcct
    30001 gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat
    30061 ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat
    30121 cggaattagt ggctttctat tcggatggct tcctcgaatg ggtaaacttg cttatatcta
    30181 cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt
    30241 ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc
    30301 cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta
    30361 taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc
    30421 caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac
    30481 tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct
    30541 aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct
    30601 ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga
    30661 tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact
    30721 tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt
    30781 aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg
    30841 ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc
    30901 tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg
    30961 ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt
    31021 tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga
    31081 ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct
    31141 aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt
    31201 atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc
    31261 ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca attttttaaa
    31321 tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt
    31381 ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat
    31441 agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc
    31501 ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct
    31561 tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt
    31621 tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt
    31681 atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt
    31741 tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc
    31801 tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca
    31861 ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct
    31921 gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt
    31981 tcaatcttat ctgttggatg gctctctttg agctgtttaa atgcgtagat tggtcgatca
    32041 aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt
    32101 attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca
    32161 agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt
    32221 aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg
    32281 ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca
    32341 agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca
    32401 atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc
    32461 ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat
    32521 caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg
    32581 gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc
    32641 tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca
    32701 tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt
    32761 tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt
    32821 tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt
    32881 ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca
    32941 tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt
    33001 ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca
    33061 ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag
    33121 taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct
    33181 caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc
    33241 ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga
    33301 ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca
    33361 cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat
    33421 tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag
    33481 ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca
    33541 aaactccttt tccactatcc gcttccttaa tagccgtctg aatctgatct ggagttggag
    33601 aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat
    33661 gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat
    33721 tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta
    33781 atccttccag catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat
    33841 tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt
    33901 ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga
    33961 taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg
    34021 ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct
    34081 acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc
    34141 acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga
    34201 ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg
    34261 ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa tttctaattt
    34321 tgggaaaata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg
    34381 atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag
    34441 ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt
    34501 tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag
    34561 aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg
    34621 gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta
    34681 gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga
    34741 tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc
    34801 ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc tgcgactaac
    34861 acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt ttttggcaaa
    34921 gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt cgtgcttcca
    34981 gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg gaaactagct
    35041 gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc gctgatcttt
    35101 ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag ctttccagaa
    35161 gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg gatcttcccg
    35221 tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt tttctcaagt
    35281 tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc gattgctgct
    35341 tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc ttttctttta
    35401 ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt tattgatgta
    35461 tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga agtcatttct
    35521 gtttccgagg caccgttttc agcaccagaa gagcttatag ttgttgaagg ttctgtttga
    35581 gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact ggaaacagca
    35641 attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct tactactttt
    35701 taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca acaggctgtt
    35761 tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc catgtcccgc
    35821 ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc gttatcgcgt
    35881 tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc aattttcttc
    35941 gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc atttcaaagc
    36001 agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt ccttgatata
    36061 cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat attgaccaga
    36121 caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta aaaggatgga
    36181 gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc caacgttttc
    36241 tttgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata atctcaggag
    36301 caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg tcttctgtcg
    36361 ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac atcgtatttg
    36421 ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta atgatgccca
    36481 cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga ttttggacat
    36541 ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa taattcacat
    36601 acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa atcaatttcc
    36661 tttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt ttttcgatcc
    36721 gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct ggtgtttggt
    36781 ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat ttcgtcatca
    36841 aatactcgat tgtatcttcg atcacaattt cttcattatg ggctggcacc atgatcgtga
    36901 tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt ttgtataaaa
    36961 acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga taagaaacga
    37021 gaaaaaaata cataatagac aaacaagtat ctaagaataa attacctgta tgggtgattg
    37081 agatcatttc agttccaccc cattttcttt atacaaatct gcaatgaatg tcgtatctag
    37141 atttttctct tcggagacag aataaaaacg gaccgtttct ctctcttctt ttgttccaaa
    37201 acgttccgta tacacttctt caagtaattc ttgtcttttt ttcaattttt catcttcata
    37261 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
    37321 tgaggtaatc aggaaactga aaaaatcaat acaaaaaagc cgattaagaa atacagcatt
    37381 ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg tctcgttaat
    37441 gggaaaaaga cccatataaa aggaagaaca acgatcatcc atcctaaaag agccactaac
    37501 gtttgccaaa tctttaacca gtaattacct tttgaaaaat acttatcttt caccaaaatt
    37561 gatgcaactt cttctcttgt tacttttttc atatcctcac ctcacgtttt tttgttacga
    37621 cgtaggagtt taccatacat ttcatttctt ttcacataaa gtgttcctaa atttttccta
    37681 agaaaaagaa caaaacattt ctcatcagca aatgaaaaat attttgttct ttttatcagt
    37741 tgttcttttt attttttatc tcttaattat gttgctcttt ccaatacgac aatgcttttc
    37801 attcctctag atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt
    37861 tcaaatggat ataagattac atgatcaaat tccagtcctt ttgctagact caaaggaatg
    37921 atttggcagt tttctaatcc atatgaattt tttttaatcg attcttgtag cgtttgggtt
    37981 tcctcttctg tttgggtgat aactgccgct ttttctcctt ttagagaaac caaaatctca
    38041 gctagtattt tcaacaattc ttcagaggat cgaaaagagc ggtattcggg ttcttttcct
    38101 tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca cagcataggt tttgaaaagc
    38161 tctgtgattg ctccgctaga acggtaacta tttctaagat cataccgatg gatcggcaga
    38221 tggtagtcgt cgaaacaacg cataatatca gcaaaagtag tggaagaatt aaagattgcc
    38281 tgattctcgt cacctactaa agtaaagtga gtttttggaa ataattcgat caatagacct
    38341 aattgtgcca gggtataatc ttgtacttca tcaatcaata gatagcgaaa tgctggtaca
    38401 tttacctttt caacaagtaa atgacgaata gctaatataa tgactgcttc atcaacagta
    38461 cgtgtgggtt gccaagcata agctgagcca tatcgttttt cataaattct ttcaaacaac
    38521 tgatgctcat ccacccatgc catttcttcg atttgtctgg ttatttttcg atattttttt
    38581 tgtaaaagtt tccttgcata agctacaata ctttgttccg aatcatcaga aatcaattta
    38641 ccgaataatt ccaactgcct atcttctgtc aacgagagta cctgattctg cacagcagga
    38701 ctttttgctt gtttcaaaag atgattcttc cattcgctca tcagtgcctt tttcgttgct
    38761 tgaagcctat cgatcaattt aggatgagca ggaactttct catatatttt ttcgatcgtt
    38821 tttttagaaa ggactgtttt tccattttta gtaagtccac ggataaaatt cggatgatct
    38881 aaaaacaaag gatctgattg ttttagatta tcgataaact ttttgctacg caaacgttct
    38941 gtctgctccg atacattttc tcccgtgata cgtttgaaat aagcctcttc accttctatt
    39001 tcctctgctg aaagctgtga aaccagctgt atgatggtta gattcagtgg atttctttca
    39061 cctaatgatg gtaggacatt cgaaatatag tcgataaacc gatgattagg tgatagaatc
    39121 agcaagtcat cggatgtcat cgtatctcga tataaataga gcagatacgc aatacgctgc
    39181 atgatagcag aggttttacc acttcctgct acgccattta ccaagatatt ttgacttttc
    39241 acatcccgaa tgattgtgtt ttgttccgat tggatcgtag cagtgatagc cttcatttca
    39301 ttggtctcat cttggcctaa gacttctaat aatacgtcat cttgaatagc tacagacgta
    39361 tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc tttaataacc
    39421 gtatcgattt tattttgacg aaccgtataa gaagatggac caatcatgtt attgtaaaaa
    39481 agttcggcaa ttggtgaacg ccaatcataa acaagatttt catctgcatc atctgtgaaa
    39541 tttgctgtac cgatataaaa ggattctttt ggctcattat ccagaaagtc tacaacgact
    39601 tttccaaagt agggactttt caataaacgt tcgatctttg ctagctgatt ttctgcgttt
    39661 ttctgtttga tattcatctg atcgatttct ctatttttca tttcaatcat cgaaaaagta
    39721 tccaggttat ctaagtaact gtcaaaattc aatcgaagct cgccagccat ttctttcaac
    39781 gactggtttc cttctatttt tgccgtatcc atcgattccc gcagttcgtt ttttttatca
    39841 attagctttt gatacacgtt cttcagatat gcttcttcta actgtttttg tgtgttgacc
    39901 atacacattc tcctcttttt ttaaagtggt ggaaccgatt agtttacctc ttttaagagt
    39961 cgaatgcaag ctttttaacg agtatcattt gttaggatat atcagttctt caggtcggac
    40021 agtttgatat aaagaaaatg caccatcaag gttttttacg tggtagccag actgcttcaa
    40081 gattctttcc gctagatagc tacgtaatcc gctatgacag ctgacgatat attcttgttg
    40141 gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt
    40201 gaatttgccc ttttccaatt cttctgctgt tcgcacatct aataaaattt ttccttttgc
    40261 tagttctgtc ggcaattcat gccattgtac ggtttcacta agcccttctg caatgttcat
    40321 tgctgcatat cctagcatgt ttactggatc tttggctgag ccaaatggcg gtgcatacgt
    40381 caattctaat tccggtaaat cgaagatcgt caaatgccct ttgattgctg ttgctaaaat
    40441 atcgatccgt ttatccacac cttttgcccc aacaccttgt gcaccataaa tctcgccagt
    40501 ctctggatgg aagatcaact tcagtaaaat atctgttgct tctggataat agcttgcatg
    40561 atcttttcct gatacatgaa tgacttcaaa agacagttcg ttttctttgg ctgttctttc
    40621 acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt ataattgctg ttccaatacc
    40681 gccttgattt cttcgagaaa ttccggaaat cgtgtccgca acttgtctac cttgacgatt
    40741 ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg atttgctgtt ttacaacgat
    40801 agcatccccc accgcataaa tatcaggaat attcgtttga taacgttcgt ctactaggat
    40861 acccccgcgt atgcctaatt cgattccagc ggtcactgct agctggttat caggacgaac
    40921 accaatggat aaaataacta aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg
    40981 tttcccgtgg tcttcaaatc cagcgacagc atgagaagta atcacttcta ctccttgatg
    41041 agacaattct ttttctataa aagctgccat ttcttcatcc agtgttggaa gaatatgtgg
    41101 tgcttgttcg atcaccatga cttctaaacc tcttcttttt aggttttctg ccatttccaa
    41161 tccgatgaat cctgcaccaa cgatcacggc acgctttggc tgtttttcta atgcatgcat
    41221 cactttatcg atatctggta catttctaat agaaaaaaca ttgtcggctt ctgttatccc
    41281 tggaatcgat ggaacaactg gttttgcacc aggagataaa accaatgcat cgtactgttc
    41341 aatgtgtttt tgaccctcat gtacgacttc taccgttttg ttttcgggaa agatggctgt
    41401 cacttcatga tttggacgca catctaaaca aaaacgagcg gataatgatt ctggcgtttg
    41461 gacaagaaga ttttcccgtt cactgatttc tcccgaaaga taataaggca agccgcaatt
    41521 tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt tctgcatctt ccattaatcg
    41581 gcgaagccgt gtagcagcag acattccacc tgctacacct ccgacaataa cgattttcat
    41641 cttatttccc tcctactgtt cgatcaaacc attgattcat gccaccacgt acattcacta
    41701 catcataacc agatttcttt aattctttag ctgcttgttt actcctcatc cctgattgac
    41761 aaatcacata gacggtcttt tctttgtctc catcatatct gtttatactt tgtaatggaa
    41821 catttttagc tccttttata tgcccgcccc ggtattctgc aggtgttcta acatcaagta
    41881 acgctggatt ttccaaaagc ttttccttta attctgggac tgttatcgat tttattgatt
    41941 gaaatagcaa attcattttc tgcacctcat ttacattttt ataaatacct catggggtat
    42001 attaaacagg agctttatta atatgtcaac cagaatgact cttcattttt ttcactactt
    42061 tttatatttg cataaaatac cgataagggg tattatctaa ttataaaaat agaggagaat
    42121 gtgttatgga gattgataat aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa
    42181 ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc atcactcaat
    42241 taagcgctgt tcgttctagt attgatcgcg taatgggcat gatcgttgca gataatctga
    42301 aaaattgctt tgagaaacct gatagtaatc cagaagaaca agcggctaaa ctagaacaag
    42361 cgatcaagat gattattaaa aagtaaagga atgatttcaa tgaaagtcgt tttccatatt
    42421 gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct
    42481 ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta tctagactct
    42541 gccaatgcag aattcttaga tttgcaagga gtcgtttttc acgcttgcgc caatgctatg
    42601 cgtgctaatc atatttcaga aagttcttta cctgaacaag taatcgttgt accagcaggt
    42661 gtccttgatt tagttgaact gcaatcccaa ggttatgctt atatcaagcc ttagtcagta
    42721 gaggttgcga cagaagtgat cagcttcaag aaatttcggc ttatttttcg aagaagctac
    42781 ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag taataagaac
    42841 agccaaacaa aaatagcttc tcatattttt cgtttggttg ggcttattac cgcagatgca
    42901 agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc taagtattag
    42961 agaaaaaaac gggaaaatag ctcctcatat tttgccgttt tttgagctaa taccgaaggt
    43021 cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt
    43081 aagaaaaatt ttgtgaaaat ggatttctac atttttgcat aaatttggct taataccgag
    43141 aaactggctt ttgaataccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt
    43201 attagagaaa caaacggaaa aatagctgct catattttgc cgttttttga gctaataccg
    43261 aaggtcagct gattgaacac cgtttaccaa gaggttgtga gcctaccatt taactccgaa
    43321 tcacaagaaa taatttcaaa aacaacttct cctgtttttg gtttggttgg cttattatcg
    43381 agaagtagct actgggagtc tatttatcaa aaaaaggaat gcgacatagg tcacacccct
    43441 tttttatttt ctttgaaaca tctttcggta ctcgacagca tacaagacaa aagattgtaa
    43501 aagaccgtaa gtgatgacgg caaaaatgac tctcacccac agcgttggta attctgggaa
    43561 aagcagcaaa aggatcatgc caacatagat gacagcagtc gctactttgc cataccactt
    43621 cgctccactt aatttgatat ttttagatag cccgattaat ccagctacaa acataaagcc
    43681 ttcttttatc aagaatagac caaacaacag ccaaagccac ggtcgatacc atgccataga
    43741 caaaatcaga aaaagttgtg tcaatttatc tgcaaacgga tcaagcacct tccctaagtc
    43801 tgttacctga ttatatctcc gagcaatgac cccgtctaga taatcagaca aacctgagac
    43861 aacaatgacc gctcctgcta tataaaatga ctgtctcagc acaaaataaa gataaatcgg
    43921 cagcaaaaaa atacggaata tcgataaaag attcggaatc gtttgtattt ctttacgcca
    43981 gtctgtgctt aatttcattt tatcgcccta cctcctcagc actcttttct ttttatttta
    44041 gcttacacgg ggaataaaac caatgatttc actgttttta tttttcttta cagccactga
    44101 tattatgagc taatttgaga taatcacttg ctgttttcat cgtttactct tctttcttcc
    44161 tgtcgtttgc gcatttcttt tactagtact ttatgagctt tttttgatcc tcgctctagg
    44221 tcacgctgcc gtttacgtgt tttataatcg ataaacagac tgtctaaatc gtaacctgtc
    44281 gggtacagat cttctgctga acgcaccaga cgaacacgct gacgaggcac gtggattatt
    44341 tttttgtcta aatatacatc aatcgtatct tcacctatat cttcataaac taacgcttct
    44401 ttttggtatt cattgacaaa tatccgatca ccttttgcaa agagctggcg ttctttttta
    44461 ttctccttcc tatttgtaac tgctgtctta ggaagaaatt cttttttctg tgtccgatag
    44521 gctttttccg ccaagtaacg ctctgcttga tggatcaatt tcatcgacat atccatttta
    44581 tgagcaatcc acaaagcttg gctttctcct gtttctccaa cccgcaactg atatttagga
    44641 cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg
    44701 aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata
    44761 gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc
    44821 tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc
    44881 atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg
    44941 aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac
    45001 atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt
    45061 gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca
    45121 tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt
    45181 ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac
    45241 accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc
    45301 aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt
    45361 tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca
    45421 tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat
    45481 tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag
    45541 aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta
    45601 cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac
    45661 tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg
    45721 tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat
    45781 tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg
    45841 atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct
    45901 cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg
    45961 attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca
    46021 aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct
    46081 gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt
    46141 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
    46201 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
    46261 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
    46321 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
    46381 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
    46441 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
    46501 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
    46561 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
    46621 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
    46681 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
    46741 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
    46801 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
    46861 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
    46921 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
    46981 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
    47041 actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
    47101 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
    47161 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
    47221 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
    47281 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
    47341 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
    47401 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
    47461 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
    47521 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
    47581 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
    47641 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
    47701 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
    47761 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
    47821 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
    47881 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
    47941 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
    48001 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
    48061 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
    48121 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
    48181 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
    48241 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
    48301 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
    48361 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
    48421 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
    48481 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
    48541 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
    48601 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
    48661 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
    48721 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
    48781 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
    48841 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
    48901 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
    48961 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
    49021 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
    49081 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
    49141 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
    49201 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
    49261 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
    49321 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
    49381 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
    49441 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
    49501 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
    49561 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
    49621 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
    49681 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
    49741 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
    49801 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
    49861 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
    49921 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
    49981 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
    50041 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
    50101 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
    50161 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
    50221 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
    50281 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
    50341 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
    50401 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
    50461 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
    50521 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
    50581 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
    50641 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
    50701 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
    50761 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
    50821 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
    50881 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
    50941 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
    51001 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
    51061 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
    51121 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
    51181 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
    51241 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
    51301 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
    51361 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
    51421 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
    51481 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
    51541 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
    51601 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
    51661 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
    51721 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
    51781 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
    51841 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
    51901 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
    51961 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
    52021 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
    52081 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
    52141 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
    52201 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
    52261 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
    52321 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
    52381 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
    52441 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
    52501 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
    52561 ttcttcccac atctgattgt cggggcgaga atgattagcc agcaaaacaa gatcatagtt
    52621 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
    52681 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
    52741 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
    52801 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
    52861 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
    52921 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
    52981 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
    53041 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
    53101 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
    53161 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
    53221 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
    53281 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
    53341 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
    53401 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
    53461 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
    53521 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
    53581 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
    53641 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
    53701 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
    53761 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
    53821 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
    53881 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
    53941 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
    54001 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
    54061 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
    54121 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
    54181 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
    54241 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
    54301 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
    54361 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
    54421 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
    54481 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
    54541 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
    54601 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
    54661 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
    54721 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
    54781 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
    54841 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
    54901 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
    54961 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
    55021 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
    55081 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
    55141 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
    55201 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
    55261 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
    55321 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
    55381 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
    55441 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
    55501 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
    55561 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
    55621 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
    55681 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
    55741 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
    55801 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
    55861 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
    55921 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
    55981 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
    56041 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
    56101 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    56161 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    56221 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    56281 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    56341 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    56401 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    56461 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    56521 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    56581 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    56641 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    56701 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    56761 atatgctgtt tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    56821 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    56881 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    56941 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    57001 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    57061 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    57121 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    57181 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    57241 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    57301 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    57361 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    57421 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    57481 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    57541 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    57601 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    57661 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    57721 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    57781 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    57841 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    57901 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    57961 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    58021 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    58081 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    58141 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    58201 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    58261 tgactgcccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacaggac
    58321 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    58381 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    58441 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    58501 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    58561 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    58621 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    58681 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    58741 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    58801 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    58861 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    58921 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    58981 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaag
    59041 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    59101 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    59161 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    59221 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
    59281 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    59341 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    59401 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    59461 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    59521 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    59581 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    59641 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    59701 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    59761 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    59821 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    59881 tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt
    59941 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    60001 agtcaaagga gacaaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc
    60061 tcaagctgat actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa
    60121 gtctcggcat ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg
    60181 gaatctatct ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt
    60241 tttgttttct ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc
    60301 caagattcaa actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata
    60361 agctcttgct cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg
    60421 aaaagataca ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc
    60481 tgttttttta gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct
    60541 tgttttcgaa aaggattcaa cgcttcggca agatacaaaa caaaataagc gctttcagct
    60601 aaattgggac gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa
    60661 ggataacggg agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc
    60721 ctcgttttca tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa
    60781 acaggaagat ctaaaaaaag aataatcgtt tgctcaaatg ctcttagatt cttctctatt
    60841 tctttgtttt tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt
    60901 gcttgttttt cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg
    60961 gtctctacca tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt
    61021 cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata
    61081 taattatatg atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa
    61141 aaaattgtac tgatttgttg aggcaacctt gcgtgttgga tcaccgtttc tgttccaaca
    61201 agagataaga aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac
    61261 gaaacttcca gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt
    61321 aatgatacgg ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac
    61381 cctttttgaa acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag
    61441 attttatgtt tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct
    61501 aaaaaataac ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc
    61561 atatttttga tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct
    61621 tcgcttgtta tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta
    61681 tttaagtcca tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa
    61741 tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt
    61801 tttaccatta gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct
    61861 aaagcttccg ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat
    61921 ttcccctgtt cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg
    61981 gcaaccatct tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg
    62041 ttttggatcg tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc
    62101 aactatgaat aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc
    62161 tagaggatca attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc
    62221 gaaaccattt gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct
    62281 acgataataa ctaaatgcaa tataatctgc cgtgttagct tgcaagcact gttcgtctcc
    62341 atctaacatt actggtgcac agtcttcttc cttccatgtc ctgtaaatat aagctggata
    62401 atgccctcgg cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc
    62461 aaatacattc tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat
    62521 aatacccatc ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc
    62581 tagaaacata tgatgacagg cttgataaat cacttttttt gttagtgtag caatgggttc
    62641 aagtttatct ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc
    62701 attattgatc tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac
    62761 ggtctgcgca taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc
    62821 tcctaccaat gctaaggggg tttcataatg actaagagta attactggct cgatatcata
    62881 tcgatttaat gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg
    62941 ttcgtctcct cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc
    63001 taactccttc agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg
    63061 agaaagataa tggagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca
    63121 ctgaatacta ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt
    63181 gccgtcttcc aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa
    63241 attttctgga aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac
    63301 ttgaagtata gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga
    63361 aaaaaattga atgctactct tcatttttaa tatttatata taacaaatac ataagacgat
    63421 ccccgaaaga aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa
    63481 atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg
    63541 acacaataac ccttaaagct tcacgtggaa cagtgaacgt ctgcttttca ctggacatat
    63601 tacaaataac gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag
    63661 gcgtcttttc cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat
    63721 cgatcaattt cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat
    63781 tgatttcttt ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat
    63841 gttcacgggc gttccattgc ataggcgtcc tagcattgtc tcttcctttc gcattgattg
    63901 attccaagat ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg
    63961 tctcaatatc ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt
    64021 ctccttgata gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg
    64081 cactttcttt tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt
    64141 gattattcca aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata
    64201 atactttttt cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt
    64261 gctgatctaa tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat
    64321 tggaataaag tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat
    64381 ttttgtcacc aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat
    64441 tacttgtgat ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc
    64501 ccccaatacc tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt
    64561 tatttttcca attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg
    64621 ttttttcatc caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt
    64681 ttccattgac cggatcacgc caaatataat agtcccgata aggattttct ttatttttac
    64741 gggcctcaat gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga
    64801 tacggatatt tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga
    64861 attctgccat gattgcttga taattactga tatcataccc attgtcatca ttcggagact
    64921 cgtatactgg agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg
    64981 aaataatccc ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat
    65041 agatttgata aacaactgaa tggtgccacc atttttgttc catgattttc ctcctataaa
    65101 tggatgtttc tattttttgc ttaatcattt attttagtct ctctcactaa aaattgtaac
    65161 gctttcaaat agctattgca ccttgttatc ggtaacagaa taaaacaaaa aaacagctga
    65221 ggaaattccc tcaactgttt tttgaaagga ttgatttatg cagggaagaa tgctgatttc
    65281 acaacttctt tataaacggt gttcactcag ctgtcccttg gtattagctc aaaaaacggc
    65341 aaaatatgag gaactatttt ttcgtttttt tctctaatac tcaggccaga acactgattt
    65401 cacaacctct ttataaacgg tgttcactca gctgtccctc ggtattagct caaaaaacgg
    65461 caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag aacgctgatt
    65521 tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac aaagaacaga
    65581 ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt aattggatag
    65641 aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat gccgatcaag
    65701 atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg caaagattgt
    65761 gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt aacaactgat
    65821 agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat agctgtgata
    65881 cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc aacaggagaa
    65941 gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc tttccctaca
    66001 cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac taaagttcct
    66061 gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat ttgaagagaa
    66121 ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact tggtgctgcg
    66181 gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat cgttgtagct
    66241 gccatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc agttacgact
    66301 aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc tactaatgta
    66361 gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat aaatagaccg
    66421 ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat catttcccgc
    66481 agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc ggctaatgct
    66541 gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc attgatccca
    66601 agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac acagccgatc
    66661 gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga agcacctaat
    66721 acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa tcctgccagt
    66781 acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag tattttttcc
    66841 ttcatcttcc atccccctaa atccgaacat tataaataaa gtccgttaaa atttcgattt
    66901 attaataaaa caacgtgttt tatcatatac taaaacaaaa cataagtaaa caaaaaattc
    66961 ggattttaag caaaatccga acgttccatc tgattaaatc cctttatctc aaagttcatg
    67021 aacattcact ttggattaca agaatttctt tattgtgtta ataaagtttc ttcattctag
    67081 tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt ctttgatgaa
    67141 ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat cgaccatttt
    67201 tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa gcttctcact
    67261 atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat ccactgtttt
    67321 tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa ttgccttcag
    67381 ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct ggttcttcca
    67441 tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc ggtggtccta
    67501 atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat tctaattctt
    67561 ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt tcagaaattt
    67621 ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg acataaagca
    67681 tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt ctttcctgtc
    67741 tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat actattcaag
    67801 aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt acaaaaaaaa
    67861 aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca
    67921 tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc
    67981 aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc
    68041 agcaccgctg cttgtgtttc ttccgtcagc attgttcttt gggcttcacg ccagttgaga
    68101 cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca
    68161 tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga
    68221 tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata
    68281 ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt
    68341 ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc
    68401 tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt
    68461 gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg
    68521 tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag
    68581 aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct
    68641 tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt
    68701 tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga
    68761 gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg
    68821 taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat
    68881 gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct
    68941 cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt
    69001 tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc
    69061 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
    69121 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
    69181 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
    69241 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
    69301 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
    69361 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
    69421 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
    69481 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
    69541 ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa
    69601 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg
    69661 ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta
    69721 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
    69781 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
    69841 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
    69901 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
    69961 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
    70021 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
    70081 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
    70141 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
    70201 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
    70261 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
    70321 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
    70381 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
    70441 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
    70501 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa
    70561 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
    70621 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
    70681 tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg
    70741 ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt
    70801 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
    70861 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
    70921 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
    70981 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
    71041 tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt taggcgaatc
    71101 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
    71161 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
    71221 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
    71281 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
    71341 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
    71401 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
    71461 cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac
    71521 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
    71581 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
    71641 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
    71701 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
    71761 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
    71821 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tagattcctt cttctgtacc
    71881 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
    71941 tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga
    72001 agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
    72061 caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
    72121 catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc
    72181 ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac
    72241 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
    72301 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
    72361 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
    72421 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
    72481 tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa
    72541 atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa
    72601 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
    72661 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
    72721 acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat
    72781 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg
    72841 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
    72901 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
    72961 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
    73021 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg
    73081 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
    73141 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
    73201 atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
    73261 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
    73321 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
    73381 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
    73441 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
    73501 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
    73561 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
    73621 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
    73681 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
    73741 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
    73801 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
    73861 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
    73921 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
    73981 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtttttac
    74041 cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc
    74101 ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac
    74161 tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat
    74221 ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa
    74281 aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcac cgcatacgaa
    74341 taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac
    74401 tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt
    74461 ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatgcg agtaggagtc
    74521 tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat
    74581 tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc
    74641 atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg
    74701 aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc
    74761 tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt
    74821 caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc
    74881 gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat
    74941 gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg
    75001 tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat
    75061 cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag
    75121 attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc
    75181 gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg
    75241 gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat
    75301 cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac
    75361 ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg
    75421 gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc
    75481 agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga aaggtacgtg
    75541 tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc
    75601 gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct
    75661 ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga
    75721 aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt
    75781 cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc
    75841 ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt ccaccaaagg
    75901 aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa
    75961 gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc
    76021 tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac
    76081 cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc
    76141 ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc
    76201 aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa
    76261 tcgagcttgc ttttcctttt ccagcttcaa caaaaaacct ttgttgcgaa ttgtacggat
    76321 ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct gacggatcgt
    76381 ccgctgactg acattgaact cacttgctag atgttttgct tttactggat attgggctgt
    76441 catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt
    76501 tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa
    76561 agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa
    76621 agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa
    76681 agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc tatttttcgt
    76741 ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg agaggttgtg
    76801 acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt
    76861 acaaaatcgt gccttattgc cgataggcta tctctggcac accgtttatt cagaaaaagg
    76921 agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata
    76981 atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat
    77041 atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga
    77101 tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc
    77161 tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct
    77221 gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt
    77281 ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg
    77341 atttccagaa actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc
    77401 gctttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct
    77461 actgacaaaa cagctaatgc aagaaatccc gtttgctcat aactagaagg acctgtacct
    77521 ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata actgccccca
    77581 tatgaacgac caccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca
    77641 tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac
    77701 aaaatgattg ctaaataatt gatttttttc attgtcttcc tcgttatctt ttattacaaa
    77761 aaactatttt tatgatagcc ttgctaaaag gaagagtcaa taaccagatg cataaactgt
    77821 ttttttagca attttgtctt atttagtcat tgagtaacat cggacaaatg tatgtgtcgg
    77881 cagtccataa ttcctctatc ttcagcatga acaagcttgc agacaagaga tctgcgcttc
    77941 ctccaggaga gatttggttt tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg
    78001 gtaaagcact ttgactagca gcatgagcaa gtttttgcat ttccactaac gttttctgat
    78061 cactgcgatg caatacgcat gtgtcatcta gcgaactcat caaaactaac aacatatgca
    78121 tttgtttctg tacatatgaa tcattatcta gctccttagc tgcttggatt gcaagcctga
    78181 tatgcggata ccctaatatc gcttcttcat atgcaccatt tacgccatat ttttgcttca
    78241 ccctatgacc atgagtggtt tttttatgac gatattttgt atcaggaaaa gcagcaagct
    78301 tgctgacatc atcaagtatt ttcgataaag aaaaaggctg ttccatactg attttttgtg
    78361 caatgacact gatcaatagc cccattgccc aaatcgctcc tttatgggta ttgatgccat
    78421 ttgtagcaat cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt ctttctctta
    78481 actcctgatc aatagcatga tgccatgcta tttgagccat ttcttcaaaa aaaggagtta
    78541 gcgtcttagc agacttaaga aataaagcat agtccatatc ggaatgtgca cccgcatcca
    78601 gtgcatccac taatccaggt tttggactta atgttacttc tgcgatcaat gcttcctcta
    78661 ccaacgcagc gagttgctga gtcactgctt tttttcgttc gtcatttatc atgacactca
    78721 ctccttcttc ctactattac ttgctgaaac cttttttgat agctacccaa atgatatgaa
    78781 gtaaccagct taaagcaaat gcacaacttg ccgtcattag ataagctaca agaatcatga
    78841 ctcctgaagg taactgcagc caactatctt taaacaataa taacagtact tggaaaacaa
    78901 atacattggc taaatacgta cgataagcat aaacagacaa ccatttgaac aatggcaatt
    78961 ttggcgaatc gtttacaatc attttggaag cgcctatgaa gacagcaaag atgatgacta
    79021 aactgtataa actttggatc gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg
    79081 gatgtgcgag caatgaacgg acagaaaaga acgcgataac taaggcaaca ggtaagaaaa
    79141 gataacgtac tctgtccaaa tattgataga ctttttcgtg ataaacaaat gcggctaccc
    79201 cataaatcat gaaactagca acaaaccgat ctaataagta ccagctgtca tgataaggcc
    79261 cagtcaatac ttgtgtttga tagaacagat accatgcgac atataaaatc gttgaaccaa
    79321 tcaatactgg ccatacatat tttttattag caaatacttt gtaaccgagc caaacaaaaa
    79381 acggcatgaa caattggatc tgcagcatca taacggtata ccataaatga ggcgctccat
    79441 cacctgtgac atatttcagt aaaaagcttg cagtatccgt atacggagtt tgttgttgga
    79501 ctgctggaaa tagccaaaga taagccgtcg tccatagaag atacggaacg actagttcaa
    79561 aaaacttttc tttcaaaaat tcccaataag acaaatgctg acttgttttt accatattat
    79621 aaacaattgc aaaaataaac attggtgctg tgtatttaac gatgacgtaa aaggtaccaa
    79681 gaaaagctgt atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca
    79741 tcataacagc cgttacagct gatactttct ggaagtcact tacgttgatt ttgatttttt
    79801 tgttttcaaa catctctttc tctctctttt tctttattac caaagttctt tggttaattt
    79861 aggtccttcg actgttttca atagaatttt tttctctggc tctctcaaat actcttttag
    79921 tgaaaaaccg ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg
    79981 cgattgaatt acctgaattg gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa
    80041 atcactggtt tcttttactg ccggaatccc agtcgataat tcaaatccta agctgccgcc
    80101 gattccccat ttacatcctg ataatatgtt tctagcagca tgaaattgct gataaacagg
    80161 ataatccatg attttctctt gtctatacga ttcttgttcg atgatgtccc acggatgaat
    80221 gacttgtttg acggcatgtt ttgagatttc tgttgaccat ctctgattgc gggcaggtcc
    80281 acgtaaacca atcggtagta tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa
    80341 catcttttca gttagaacga cccattcagg caaggtgaac ccttctattt catgtttatc
    80401 aaaaaggaca atatcatgtg cttgaatcat tttttatgca tcccattctt cgtccatttt
    80461 ggcacggact ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg
    80521 atttgtcagc atcgtgtcat ttgtattgcg gacatcttga atggcttcat tgattgcttc
    80581 gatgactttg ctgacatttt gttctgaagc agcttggtcg gtgatatctt ctaggaaatg
    80641 atagatcgct cctagttttg tataattatt gatatcgtaa gcgatagctg gaactttttc
    80701 agcagctgct tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac tttctttaga
    80761 catcgcttgg acggtaattt ctttattgtt taaagcaatc aagcggcttg attgtaatcc
    80821 atgtgctaaa aatgcacctg aaatagcatt tcctgcaatc aatccaataa ctggatgtcc
    80881 agcttgacgg agttttgcat aagcagcagc actattagcc aaagccacat ggataccgat
    80941 caattcttcg ttatagccgt atgcttggct tggtacatcg atcacaacga tgattggccg
    81001 tttcactttt ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa
    81061 accttcaact aaaccaactt caccgtttcg tacacggtac atcgggtttt taggatcagg
    81121 tacgattgat gtcacgatgg cttcttctat gaatttactt gatttaccat aatacacagt
    81181 actaatagaa gaagttggat ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac
    81241 acttgctgca gctggttcat ttccttctgc tacttctggt aagtcaattg attttgcttg
    81301 atggttagca attgcttcat tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga
    81361 atcaagtaat gataaataaa atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc
    81421 cattgctgcg atgacagcag ctttgattgc ttctacacta tcagtcacta attcatcaat
    81481 gatttttgat tctacccgtt gacgagatcc taatgtattc caaatcaagt ctttgtcact
    81541 agaatcaaat tctcgaacgc ctgcttcttg ttcaataact tctggtccat tcaaaccgac
    81601 tctcgctttt tttgttgcga ttaaataact cattaatgct gaagtaatcg acataccgcc
    81661 aaatgatcct acacgtcctg gaatcaaacc gatgactggt acatattgtt ttagagcaat
    81721 gattacattt ccgatttctg aaatagataa cagtccgtag tttgcttctt gcaaacgaac
    81781 accacctgta tctagtacaa tgactggaaa gatctcattt ccttttttat tttcttctaa
    81841 cacatgttca agtgcagcaa cgatttttgc accggaaact tcaccgatac cgccaccttg
    81901 gaattttcct tccatagcga ttactactgc ttgtttgcca ttgatcgtgc cacggctgac
    81961 aacgatcccg tcatcacttt cagggacgat tccttgtgct ggtaaacttg gcgcgatgat
    82021 ttgatcgaat ggatcaatca attcacgtcc attgtcaagt agagcgttca ctcgttctct
    82081 ggcgtttaat tctacaaaac tatttttcat ctgaaagttc ctccattgct tgcgtcaatc
    82141 gtaggtacac aactcctggt gtagcaccga agtcattgat cacgatatct gctgaaattg
    82201 gataacgatc aaaaaatctc gttaatacat tgttccatac ttcgccgaag ccatcgcttc
    82261 ctgtacaaat ttttacagta gattctgttt tttctgttgg aaacatcaag atttccaaat
    82321 ctcctgaacc gaccactcct acatggattt tcttattgat cggctgtttc gttggatatt
    82381 tataagctaa ttgttccatg gatctctccc ctttttgtaa aacagagcat acacgttctg
    82441 cttattcgtc tttcttcttc catctgggta aatggcgaca aaaggttttt ctcgccaatt
    82501 ttatgaccaa gttctgaatt tcattggtgg ttcatacagg ccatctgacc attcgatcaa
    82561 atcatcgatg gtttgtgcag ctaataatga acgtttcgct tcactacgtt tgatgcctag
    82621 atcttctggt aatttcacga tgccggctgc acgcaagcta tctagtgatt ctttcgtgct
    82681 agtcattcca agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc gttcttcttt
    82741 tgtttttgct ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg tagtatcttc
    82801 gctataaatc atcactggtg cattggctaa gcctgcttgt ttttgaacag ctagtgcatc
    82861 taattcttcg acaaagactg gttttttatt tgaaccatat gtttcaacca tttgaacaac
    82921 taatttttgg cctttcccta atggatcatc attgtcgcga agtgattgcc atgctggtgt
    82981 agaatgacga cgaccgccag gattatgtcc catgttaggc gcaccgccaa aaccagataa
    83041 acgtcctttt gttacggtag aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat
    83101 aaacatatcg atggcatatt gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt
    83161 gccgtcttta ccgacaaaaa agacatctgg gcgagcagca atatattttt ccataccaac
    83221 ttcgccacca aaactatgga tgctttcaac ccaacctgat tcgatggctg gaatcatcgt
    83281 tggatgtggg ttcaataccc agttcttcgc gattttccct tttaacccta gtgattcacc
    83341 ataagttggc agtaacaatt cgatagctgc tgtattgaat ccaataccgt gattcaaaga
    83401 ttgtacgccg tgttctgcgt agattccttt gattgccatc attgccatta gaatttgcaa
    83461 ttctgtgatg ttttgaggat ctcgtgtgaa taaagcttct aattgataag gtttatctgc
    83521 tttcacgaca acgtctaccc agtcaccagg gatatctaca cgtggtaaag aatcgacaac
    83581 ttcgttgact tgaacgatca cgataccatc tttgaaagct gcagcttcga tgattgttgg
    83641 tgtttcttcc gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc
    83701 tactaaaaca acatttggaa tcaaatcgat gaataagcgt ccataaagtt ccaaataagt
    83761 atggatatca cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg
    83821 tgcaccagca taagagaaat cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg
    83881 ttctggtcgt gagatactag acatgatcaa atgcagatca ttcacttttt ctgcatctac
    83941 tttttcaaga ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac
    84001 tttgtcgccg ggagcaatca gtttttccat tacttctacg attttttcag tttctacata
    84061 cttgccattg agcaagttgc tgattttttc caatcgctgc gctttttttt cgcgtttaga
    84121 agcccagttg cggtttggtt tttccataat tagtcatctc catttttgat atagaaacgt
    84181 ggcgtcatca ataccatgtt ggtctagatt gatcgtacgg atccctttat ctacaccaaa
    84241 ttttgtatga atcagctttg tcaaagtatt gccgggggga aattcaatcg tcagatccat
    84301 tcctaattct tttgccaagt ccatcatttg gttccactgc acagggaaag ctaggttttc
    84361 caacaaatct ttacgaatct cttctgcgtc ctgagtggct cttccatccg tattttttat
    84421 atacaaacat ctagcatcag atagatggac agactgcata tacggcttga atttcttgac
    84481 cgtatcattc atcaagagac agtgagatgg cacagggact ttcagcagct tcgctgtttg
    84541 cgcttggtgc cgtttggctt ctgccaacgt aacggataaa ccttcgatac tcccagataa
    84601 cgtatgttgc atcggacaat tttcatttga cagataaact ggatggtcag gatcaaatgt
    84661 ttttgctaca atttcctcag cttcgcttct cgtcacacct actactacgc ccattgcata
    84721 accgcttgga taagcttctt tcattaagac tgcgcggtga tagactagtt ccgctgcatt
    84781 ctcaaatctc aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc
    84841 tgcaatatca ggctgaatac cgtttttttc catttcatct aaataaaata ctgcttttgt
    84901 cagtaatgct agctggataa aaaccgtatc ttgatagttt tcttctttat caactaaccg
    84961 ataccccgtc acattttcta aacgttccat gtacatttca gggatatccg ctaacatccc
    85021 taacttctga ctgccttgtc caggaaataa gaaacctgta ctcatgagcc tcgcctcctc
    85081 agcacttact ttttccactg ctatcttagt caatttctgc tataataaaa aatgccaaaa
    85141 aaagaaaaaa ccataacaaa aaggtatgga tgggatggat aattatgaaa ttgcttcatc
    85201 tgcagtattt taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat
    85261 ttatttctca acctgccttg agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc
    85321 ctttgtttta ccatagcggc agaaacattg agacgaccgt ttatgcagaa gaattttatc
    85381 catatgcagt caagacatta gacacattag aagaaggaat caaattattg tcttcggtca
    85441 atgagaagat cgttacttcc gtcatattgt atttagaggt agcctctgtt tctattccga
    85501 acttggtccg aatttttatg gaaaagcatc ctgatatcca attaacgatc atgcaacatc
    85561 atttaccaga tgatattacc gatcctgtac tgtacatcac ttcggagatg aagcctggca
    85621 tgaccaatat tcctatcatg aaagaacctg tttatgtcgc cattcctaag aaacatcctc
    85681 ttgctaaaaa agaacatctg caattagaag atatccaacg cacccccatc ttgatgctgt
    85741 caaagagcaa cgccttccgt catacactgg atgcagcact tgaagcgaaa agtatcgaat
    85801 tgacgatcag ttcgacgaca gatgatcctg cgacattacg ttctattgtc aagcaaggat
    85861 tagctgttag ctttttccca aaagtttctt ggtcttacga taaaaatgat cctttcgtct
    85921 tgcgggaaat caaagatttg cccttgacac gaacaattta tttatcttct agcttcacag
    85981 aagatcatcc tttgatcaaa atgattgcaa aaacgatgcg ggaattttat ttaggaaaat
    86041 aaaaaaaaaa gagctgggac aaaattcttg tcgcagctct tttttcgaac aaacggtgtt
    86101 ccagaagtaa tttcttagaa aataagccga aatttcccaa aaatttgaga agcaattttc
    86161 gtaaattcct tcttatttct tgaagctaaa catttctgtc acaacctctt ctctattgtc
    86221 ctagacattc attgcgttga tttaaaactt ccatcaatca aatctgctag ttcatctagc
    86281 ttttctattt tacgtagatc aatagagatc caatgttctt tattcatgtg ataggctgga
    86341 aaatacccct cttttcctcg caaaattccg ccaagttcag gatcgacctt caaatcaaga
    86401 atattgatga tcctatggtc acttgcatca tcatagattt tttcttctgg aacagacatc
    86461 aggaccccaa accatttttt gcttttttga tgtctgaata cgcaataatc aggaaatttt
    86521 ttaaaaatat attctggaga caaattgtat gtttcattga tgtgatcgat tagttctttt
    86581 ttcgttagca tctgcttcca tctctttctt attgattcgc tttattcaaa gataccacgt
    86641 aagaatagaa aaatgaatac aagtagttgc taatcaaata cttcgatatt tttctttttt
    86701 atcaactctt aaatgttcaa tccgcctgtg ttggtcttgc taccatctac tattaaaatg
    86761 tttattattg tttgcccacc tattttttga ttttagtggt atcatttttc aagttcatga
    86821 tgaaattctt atcatcagta aatgctccag aaaaataaac aatgattgaa aaatcagttg
    86881 aaacttgacc accgtttatt ttctccgtat tcttagaata gaagggagtc tgattgatgt
    86941 attcactaat gaaaaaaatc attaccgaaa aagatattcg acgtcatgtt tctttagtag
    87001 aacaattgct gaatcataca aagataaccg taaatgaatt agctgaaatc atagggacaa
    87061 ccgaacgaac gattttttct gaccttcaat ctattcgctc tcagctgccc gaaggctggg
    87121 acatcatcag cgatcaagca ggaatcagtt tgcaaaacca gcaaaattta ttaacaaatg
    87181 acctttggga gatttttttc aaacaatcag tcagtgtcga actgctgaaa aatctgcttt
    87241 ttacaaaaaa agttgctgtc cctgattttc tagccgacca tgggctttct tatgggacat
    87301 tgaaaagaca tgtgactaaa atcaatcagc gtttagcaag ttacgacttg cagatcgatt
    87361 tgacgaaata tacagcttgt attcttggaa aagagcgtgc gattcgtact ttttatcatc
    87421 gattgctgat tcctttcact cataacaact attttttcga ggattattcg atccatgaat
    87481 cccattattt tcaattttta agaaacctca gtcaaactga actagcggtt gaaacagaag
    87541 agattttcgg aacatgttgg ttctttatca atacgatccg tataaaagcg aattgccgat
    87601 tagattcttc tatccatacc aacagcacac tctcctccct gtatgactct gcattgaaaa
    87661 aattgtattt aacggaaggt atttatctta aagacactga gctttctttt gcttctttct
    87721 gctttctgga aagttggaat tacaacaata actatggaca agaaaccgct cgctgcctgc
    87781 atcattctcc atttttagag gtcctcgaaa cgtttgtaga ggaactagct tcagagctgt
    87841 ctcttgatca actaaaaaaa acatctctga ctgataactt ggctctcctc atattaaaat
    87901 atcatgaatc gccgatattg attgaagcat tggaccgtca ataccatcgt tttttagaaa
    87961 catatgatca gcaatatgca cacttgtatc aacagaaaga gaatttactg aacgatctaa
    88021 aaaaagaaat agaaatcaat gaaccaaatt actttctcca actcttgtct ctactgatcc
    88081 aacaaacgat tttttctatc aaaccgaatt tgttcaatgt ctattttatt ttccaaggag
    88141 aaccttcatg gaaggcgttc ttgcagcagg aattaaaaga ttacttagga aaacgtgtct
    88201 gcttcttacc tatcgaacta accgatcttt ccactatttc ttttgaaaaa acagatatct
    88261 tgatttccaa tttccctcta gattctctag atatccctat tgtatacatt tcttctattc
    88321 caacgaaaaa tgagctcaat cgtttgactg aactcacttt caaaactttt ttatagtaac
    88381 ggctagcgac tgtattctct agctgttttt tcttttttgc gaaacagttt gttcatgatc
    88441 cctttttcta tttctacaac taagaataga agaatcacat ttgctccaat caataactgt
    88501 tgtgtcatcg ttaaacttac tgtaccgatc agctgctggc cgactggtag aaaaataacc
    88561 atcccttgca ataaagcaag gatacctaat gaaagaaaaa gcgctttgtt ttgcaaaaga
    88621 ccttggttca atgacggctt ggacaactct ctgcaattga tcatgtaaac agcttgagct
    88681 aagacgatat tttgcagaag aatggtttgc tgtaagcttt gcccttcaaa ctgcatagcc
    88741 aataaatagg caggaaccat gatcagtagg gagacataga tgatccgaaa caaatgataa
    88801 ccagatagga ttccttgctt tggatctctt ggtgggcgct tcatgatttc tggatctggc
    88861 gactcgaacc ctagtgcata agataaagtg attgtcgtta ccatattgac ccataaaatc
    88921 tgtactgggg acaaaggtag cgggcgattc agcataagtg cccaaatcaa gatcaatcct
    88981 tgagctaatg cagttggcaa gaagaaattg atggtttttt gcaaattatc caaaatccgc
    89041 cggccctctt ttacggcttt tgcaatcgta tggaaattat catctcctaa aaccatatca
    89101 gctgcctgtt tgcttacttc actacctttg attcccatag caattcctac atcggatctt
    89161 ttcaaagcag gtgcatcatt tacgccgtct ccggtcattc cgacgatttc tccatttttc
    89221 tgcaaagctt taacaattcg cagtttatgt tctggtgtcg ttcgagcaaa gacatcgact
    89281 attggcactt gctgaatcaa ctcttcatca gacataagat ccaaatcgat cccttctaaa
    89341 actttggcag tatgttttaa gccaatttgt tccgcaatcg cttttgctgt gtctttgtga
    89401 tcccctgtaa tcattttcac tgagatccct gcttgttggc attcctttac agctttgact
    89461 gcactttctt ttggcggatc gatcaatccg gcgatccctg caaaagttaa ttgggttaaa
    89521 gtctcatgcg tgagttcctg tttcgaatct acttttttat aagcaaatcc taaaacacgc
    89581 tgaccttttc tcgcaaattc tgcagctgtc tgactccatg cttcttgttc tgcagaagaa
    89641 agagttgatt tagcaaataa tacttctggt gcacctttga cgtaaatcac cgctcctttt
    89701 tcctccgcat gcatcgttgc catatatttg tattcagaac tgaacggaat ctttgcttct
    89761 atttttttca aaggtagatt ggctgtttct gtgtgtcgaa tcagcgcaac ttctgtcgga
    89821 ttgccactta aattttctgt tttctgttct tcttttgttt ttaattcctg acagtttttc
    89881 ataatggaca acacgcaggc ttcttctttt gcagctacat ccacaactgt catttcattt
    89941 tttgtcaatg ttcctgtctt atctgaacag atcactgtca tagaacctaa tgtttctacc
    90001 gaaggaagtc ctttgatgat cgccttttct tttgccattt catgtactcc aacggataga
    90061 atcatggtca aaacagctgg caatccttcg ggtaccattg acaccaccaa agcaatcgta
    90121 gccgaaagaa ggatatgcca atccatacca tatcgaaatg tcgtaaaaat aactaagaat
    90181 aggctcaaac aaagaatgcc ttgaaaaatt tgtttgttta atcggttgat ttttttaatc
    90241 agaggcgtcg tttgttgttc gaccgattgc aatgcttgat tgattttacc gatttctgtc
    90301 gtgtttcctg tttctacaac gacaccgact gcagaaccag attgtacaag cgttcctgaa
    90361 aaagccatat ttttctggtc gccgatcgat tcttcttctg tcaaaacggc atgacttttt
    90421 tcagatgctt ctgattctcc cgtcaaaatc gattcttcta ctaataaatc atgtgcttcg
    90481 attaatcgga tatctgctgg aacgacatct ccggcacgta agacaatgac atctcctttt
    90541 accagttctt tcgcagggat tttttcttgc atcccgtttg atagtactat cgcttcttgt
    90601 cccatcagct gcttcaaacc attcaatgat tcttccgctt ttctttcttg ccagtaacta
    90661 acgagtccat tgacgatcac tacgaataaa ataatacttc cttcaatata ttcactagaa
    90721 gcaaatttca aaaaagaggc aatgatcaac acgatcatca gcagatcagt aaaatgcttt
    90781 gctatttttt tccatcgttt tgtctgctgc cttgctttta tttcattata tccttgatca
    90841 gtcaggcgtt tctgtcgttc ttcttgcgac aagccttctg ttgatgtttg atagtttttc
    90901 aatacatctt ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt ttaattgata
    90961 ctgttattat acgcaggaaa gttttttcag gcgtatcgat tatttcaggg gggtctgaaa
    91021 taattcgttt tgcaaaatgt atctgggcct aactgatatt tctattgctt attcttttat
    91081 atacaaaatt taagaataca aaccagataa ccaataaaaa aggaataaag cgtgtcgtgc
    91141 tttcgaataa caagctgaaa gcaattgaag taaaaaacac caacaaagca agatctttat
    91201 atctttgtgt agtgttccgt ttcttttctt taataaattg aacatgtgca cacaagatcg
    91261 aagaccacac aaatagaaaa caactcgttg tcatgcctgc caaaagctga aaaaacaatc
    91321 gactatcagc tacaaaaaaa tgtagacaaa gaccggcaaa aaataacatt cctgtaatta
    91381 aaataccgtg taaaggtacg cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt
    91441 tttcttttgc tgtttgatat aaaatcctgc tagtactgta gactgcacta tttgctgagg
    91501 acaaagaagc aaccaaaatc attagattga caattgctgc tccgccttgg caatggatca
    91561 cttgcagcat aatcactaaa ggactttctt ttaaatctaa acaatgccat ggaacaacaa
    91621 ataaaatcga caaaatcgtt cctatataga aaaagagaat acgccaaggc agacagttga
    91681 ttgctttttt caaattcttt tgcggatcac ttgtctcgcc aacggttaag ccgatcattt
    91741 caattccagc aaaactgaat aaaaccatag gaaatgagac taccattcca gcaaagccaa
    91801 acggtagtaa ttcatcgatc tgtccaaaat tttctttgat tccgatagac tgggtatgaa
    91861 aatctgagac actcaatcct atacccaata agataaaaag gaaaatcccc aagatcttga
    91921 tgattgagaa aaaagattcc aattcaccaa accacttgac tgcaacaagg ttgactgcca
    91981 ttaagatcat tagtgcgcta cctccgctta tcaaagctgg tacatgtaca aaccagaaat
    92041 ggatatacaa tcctattgct gttaattccg caagtccagc tccaagccag caaagccaat
    92101 aagtccaatt gacaaaaaat gcccactttt tgcctaaata atagtcaacg aaatcactga
    92161 tagagttaaa tgtactattt gctaaaaata aatcacccat ggcccgcatc aaaccataaa
    92221 tagccaaacc gcatatcaaa taaacgagaa taacagctgg ccctgctgat gcaattgcct
    92281 ttgctgaacc taaaaataat cctgttccaa ttgcgccacc taatgcgatt aactggatat
    92341 ttcgattagt tagagtccgt ttcattgccg gtttttccat caagttcacc tttttctttt
    92401 tcaagcagca tggcgacttc ctgttcccaa gaaggtttct ttattttttc aatacagagt
    92461 ggtactacaa acaaaatgac actgatggct gtgactgcaa ttagattgat ccatgttcca
    92521 gctttcatcg tcagtccgac agaaaatccg attgccgcaa ataaaacaat aacgacagtc
    92581 caaactccaa gattgctctt tttctctttt tttccaattc taaatggtcg attcaacttt
    92641 gcatgtgtat aacgcaatct caaaactgcg atccccatga tgacatagac gatacagtag
    92701 ataacagtag ttgaatttac gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa
    92761 taaaaaagtg caaagattga gataatgaca gactgagtca aaataacgga tttggatacg
    92821 tcaaattgat tcgtcttcca aaaattgaat tttggtggat acgcaccttt tctagcactt
    92881 tctgtcactg tctttgctgg tcctgaggcc caagcagata attggactgc tacaccaatg
    92941 aagacaagta aactaaacag attcacaata agatgtggca aaccaagaat ttggcaataa
    93001 atcgaaatag attgcgcgat attattcaat tccatttgtc cttttggtac gacatttgct
    93061 acaactaaag cattcaaaat attgacgaaa aatataaaaa ttaacgcggc aaatactccg
    93121 cgaatatacg ttttaactgg attttccaat cgagtgatat acacagagga catttcgata
    93181 ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct
    93241 ggtattaatt tgtcccatga aaaactgcct aatgttccgg ttggtacgat cccaactttg
    93301 atccacgcag caaaccctaa taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa
    93361 acaccgactt ttccgccgat ttttgccata tcaaatttca aattcaaaaa agtgatgatc
    93421 caataaacga ctaaaatgac aactaatgta aatttgctgt tcaaaccaag cggcacatta
    93481 tcgataatat taccaaacaa cggagcgagt gctgaagcga ccatcaccat cccaggaaac
    93541 atttggaccc ataacagcca tgagacaacg aacccccatt tttttcctag agcattcgat
    93601 acccaaagtt ctggaccacc tttttgggga aacattccag caaattcacc agagatcaat
    93661 gagattggaa aagcgtacaa taatactgca accagcatgt aaaaaatcat cgtccagctc
    93721 gttgctgcta catctggaat atttcgtaca cttgctacaa gggcgcaggt cattccgata
    93781 aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt tcatctttat tccttctttc
    93841 aatagcgaca tatgtttttg ttttctttta tttttgttca agaacagaaa tgacaaggtt
    93901 tcatcgattc agtacaaaac ccagtatcac cgaaacatcc attgaacgat cttagtgaac
    93961 tgggtttgat acatgaaacg aacgatccaa cttatagaac gttttataaa aaagaatatc
    94021 ttggcgagga atatatgaaa aacaggggga aatacaattg ttttgatgaa gcaaataatt
    94081 gtctgttctc catatgtctc ttgttattct ttcacacaaa aatcagttct attggatgat
    94141 ttcgtttagt ttcttttcca ttgctttggc gatttttttt gtataaaaat tgaaaattcg
    94201 atcatcattc aaataaggag tcatgatagc cgcacgtagc agagtgattt tctgttcttt
    94261 tttccattct tctttcgtta gacccattct ttcaacaaaa cgaattggag aatcaccgta
    94321 atcttcttgt gtaaatgtag tatgagaagt aataaaccgt tcaccataaa catcgccatc
    94381 catataagaa gaccgatcga acattttttc atttaattca ttgatagcgt tcaaatctgt
    94441 acagccttgt tctttgaata cccaattgac catattaaaa tcaggatgat tcaatggata
    94501 gacttcgatt gtttttcctt taacagtaaa agtcagatga tcgagaaatt ctctgaatct
    94561 ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa
    94621 tacacgatgt gctgtccaga ctgcggcggc ggttgctcca gcttttgatc cttccaaaat
    94681 ataagctcct aacatatctg gagcctttac tgatttttca aacacatatg gcgcgaaata
    94741 agaaatgata tttctcatat ttttatgttt gattacgatt cctccagcag cataagggac
    94801 atagcccatc ttatgaggat ctatcgtgac agaatccgct tctgaaatag ctctgaaccc
    94861 ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa
    94921 ttcagctaag gaagcatacg gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc
    94981 atagccgcca tatgcggcat caacatgtaa atagaaatag atcccttcat cttttaacct
    95041 ttctcttaat tggacgattt tatctacact gtctacttga ccttcttctg tcgttcctac
    95101 gacagcaacc acacctagaa ttgggatttt ttgtccagct aattcacgaa ttgttttttc
    95161 caaagcatta atatccattc gataatcctc ttgaactggg atagcaacca tctgatctaa
    95221 gccgactccg caaatatcta acgccttcat ccaagaatag tgttttgttt gagggaccag
    95281 ccatttccct aatctttgga tattttttcc gctacgggaa gaagctgctt tgacctcatc
    95341 catttcttcg tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag ataaattcaa
    95401 aagttcccac tcagacattt ttttcacttt ttcaggcagc acttccttga cagctaaagg
    95461 aatcgatttg atgcaacggg cgtaccataa tccttcaaga ttagcaatag aaccatctgc
    95521 tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc
    95581 tgcttccatt tgcgaagttg ccatagaaga ttccaatgca acattatttg ggttccacag
    95641 catcgcatag ttgtaagcta ataaagcagg catcaatgtt tcagcattca tttgtcccca
    95701 gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac tgacttcatc
    95761 caagacctct atcatatggt cgcgggtggc tagataatct ggagtgagct tatctccttc
    95821 gctaattgca ggcatatctg aaggaatata attttctctc catccaagat gttcatctac
    95881 catcttgttc aacaacattt tataaactgg tccattttca gctttgtcgc ctataaagac
    95941 ggccttgata tccatatctt tcatttttct tccctccata ttttgttggt caatgtcttg
    96001 cagatacatg tgtttatctg taacacctct tcactatagc gccaaaaatg agaaattttt
    96061 ttttataaca tcaaatccac gtagattttt acaaacaaaa ataaataaag tcggattttt
    96121 ctgataattc aacgcaaaaa agcgaacaat ttttcaataa atcagtcttc taaaatagat
    96181 aacctaaaaa aagcgaacaa acaaataaaa tgactgtcct tcattcacta aacatatttt
    96241 tattttttta tattgttaaa aattcacaat ttatttttta atagaattat ctgttttcca
    96301 aattttata
//
LOCUS       DAGCXG010000002        90754 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.002, whole genome shotgun sequence.
ACCESSION   DAGCXG010000002 DAGCXG010000000
VERSION     DAGCXG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.002"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..547
                     /locus_tag="MB022_000082"
     CDS             <1..547
                     /locus_tag="MB022_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465114.1"
                     /translation="IFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGN
                     KRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSI
                     FLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNE
                     FMQFGLTVLGICTFIITVINR"
     gene            complement(787..870)
                     /locus_tag="MB022_000083"
     tRNA            complement(787..870)
                     /locus_tag="MB022_000083"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(834..836),aa:Leu,seq:gag)
     gene            925..1104
                     /locus_tag="MB022_000084"
     CDS             925..1104
                     /locus_tag="MB022_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465115.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1253..2470
                     /locus_tag="MB022_000085"
     CDS             1253..2470
                     /locus_tag="MB022_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4465116.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2654..3490
                     /locus_tag="MB022_000086"
     CDS             2654..3490
                     /locus_tag="MB022_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4465117.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3566..4714
                     /gene="nagA"
                     /locus_tag="MB022_000087"
     CDS             3566..4714
                     /gene="nagA"
                     /locus_tag="MB022_000087"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBT4465118.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4935..5993
                     /locus_tag="MB022_000088"
     CDS             4935..5993
                     /locus_tag="MB022_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBT4465119.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            5997..7031
                     /locus_tag="MB022_000089"
     CDS             5997..7031
                     /locus_tag="MB022_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBT4465120.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7133..7849)
                     /locus_tag="MB022_000090"
     CDS             complement(7133..7849)
                     /locus_tag="MB022_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4465121.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7818..8603)
                     /locus_tag="MB022_000091"
     CDS             complement(7818..8603)
                     /locus_tag="MB022_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBT4465122.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8594..9343)
                     /locus_tag="MB022_000092"
     CDS             complement(8594..9343)
                     /locus_tag="MB022_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4465123.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9641..10165
                     /locus_tag="MB022_000093"
     CDS             9641..10165
                     /locus_tag="MB022_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBT4465124.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10181..11680
                     /locus_tag="MB022_000094"
     CDS             10181..11680
                     /locus_tag="MB022_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBT4465125.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11768..12370)
                     /locus_tag="MB022_000095"
     CDS             complement(11768..12370)
                     /locus_tag="MB022_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4465126.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12474..13430
                     /locus_tag="MB022_000096"
     CDS             12474..13430
                     /locus_tag="MB022_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBT4465127.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13735..14691)
                     /locus_tag="MB022_000097"
     CDS             complement(13735..14691)
                     /locus_tag="MB022_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4465128.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14865..15425
                     /locus_tag="MB022_000098"
     CDS             14865..15425
                     /locus_tag="MB022_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465129.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15418..16242
                     /locus_tag="MB022_000099"
     CDS             15418..16242
                     /locus_tag="MB022_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBT4465130.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16252..16869
                     /locus_tag="MB022_000100"
     CDS             16252..16869
                     /locus_tag="MB022_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4465131.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16887..17570
                     /locus_tag="MB022_000101"
     CDS             16887..17570
                     /locus_tag="MB022_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4465132.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18253..19536
                     /locus_tag="MB022_000102"
     CDS             18253..19536
                     /locus_tag="MB022_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4465133.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19552..20439
                     /locus_tag="MB022_000103"
     CDS             19552..20439
                     /locus_tag="MB022_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4465134.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20511..21287
                     /locus_tag="MB022_000104"
     CDS             20511..21287
                     /locus_tag="MB022_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4465135.1"
                     /translation="MIYPVLWMIAGSFKTDQEIMSGSLNLIPETFQWENYAKGWAGFA
                     GISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDFKGKRFWFVVMLVTMMIPAQV
                     ILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQFMVSIPKELDESAIIDGCSK
                     YSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYLNKPRSYTVSIAIKLFADAGS
                     STTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGLKG"
     gene            21290..21913
                     /locus_tag="MB022_000105"
     CDS             21290..21913
                     /locus_tag="MB022_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465136.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21910..23202
                     /locus_tag="MB022_000106"
     CDS             21910..23202
                     /locus_tag="MB022_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4465137.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23561..23658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23774..24355
                     /locus_tag="MB022_000107"
     CDS             23774..24355
                     /locus_tag="MB022_000107"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBT4465138.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24358..25665
                     /locus_tag="MB022_000108"
     CDS             24358..25665
                     /locus_tag="MB022_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBT4465139.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26081..26569
                     /gene="purE"
                     /locus_tag="MB022_000109"
     CDS             26081..26569
                     /gene="purE"
                     /locus_tag="MB022_000109"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBT4465140.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26562..27686
                     /gene="purK"
                     /locus_tag="MB022_000110"
     CDS             26562..27686
                     /gene="purK"
                     /locus_tag="MB022_000110"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBT4465141.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27699..28994
                     /gene="purB"
                     /locus_tag="MB022_000111"
     CDS             27699..28994
                     /gene="purB"
                     /locus_tag="MB022_000111"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBT4465142.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29174..29551)
                     /locus_tag="MB022_000112"
     CDS             complement(29174..29551)
                     /locus_tag="MB022_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465143.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29807..30535
                     /locus_tag="MB022_000113"
     CDS             29807..30535
                     /locus_tag="MB022_000113"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBT4465144.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30535..30789
                     /gene="purS"
                     /locus_tag="MB022_000114"
     CDS             30535..30789
                     /gene="purS"
                     /locus_tag="MB022_000114"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBT4465145.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30791..31462
                     /gene="purQ"
                     /locus_tag="MB022_000115"
     CDS             30791..31462
                     /gene="purQ"
                     /locus_tag="MB022_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBT4465146.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31463..33685
                     /gene="purL"
                     /locus_tag="MB022_000116"
     CDS             31463..33685
                     /gene="purL"
                     /locus_tag="MB022_000116"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBT4465147.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33670..35109
                     /locus_tag="MB022_000117"
     CDS             33670..35109
                     /locus_tag="MB022_000117"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBT4465148.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35132..36184
                     /locus_tag="MB022_000118"
     CDS             35132..36184
                     /locus_tag="MB022_000118"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBT4465149.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36181..36759
                     /gene="purN"
                     /locus_tag="MB022_000119"
     CDS             36181..36759
                     /gene="purN"
                     /locus_tag="MB022_000119"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBT4465150.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36756..38291
                     /gene="purH"
                     /locus_tag="MB022_000120"
     CDS             36756..38291
                     /gene="purH"
                     /locus_tag="MB022_000120"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBT4465151.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38307..39581
                     /gene="purD"
                     /locus_tag="MB022_000121"
     CDS             38307..39581
                     /gene="purD"
                     /locus_tag="MB022_000121"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBT4465152.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39703..40617
                     /locus_tag="MB022_000122"
     CDS             39703..40617
                     /locus_tag="MB022_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4465153.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40750..42315)
                     /locus_tag="MB022_000123"
     CDS             complement(40750..42315)
                     /locus_tag="MB022_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4465154.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42308..43759)
                     /locus_tag="MB022_000124"
     CDS             complement(42308..43759)
                     /locus_tag="MB022_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4465155.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43773..44789)
                     /locus_tag="MB022_000125"
     CDS             complement(43773..44789)
                     /locus_tag="MB022_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4465156.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45009..46097
                     /locus_tag="MB022_000126"
     CDS             45009..46097
                     /locus_tag="MB022_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBT4465157.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46151..47677
                     /locus_tag="MB022_000127"
     CDS             46151..47677
                     /locus_tag="MB022_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBT4465158.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47679..48884
                     /locus_tag="MB022_000128"
     CDS             47679..48884
                     /locus_tag="MB022_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4465159.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49141..50118
                     /gene="guaC"
                     /locus_tag="MB022_000129"
     CDS             49141..50118
                     /gene="guaC"
                     /locus_tag="MB022_000129"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBT4465160.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50382..51806
                     /locus_tag="MB022_000130"
     CDS             50382..51806
                     /locus_tag="MB022_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4465161.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIESGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52267..53376
                     /gene="pdhA"
                     /locus_tag="MB022_000131"
     CDS             52267..53376
                     /gene="pdhA"
                     /locus_tag="MB022_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBT4465162.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53379..54356
                     /locus_tag="MB022_000132"
     CDS             53379..54356
                     /locus_tag="MB022_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBT4465163.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54381..56024
                     /locus_tag="MB022_000133"
     CDS             54381..56024
                     /locus_tag="MB022_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBT4465164.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56032..57438
                     /gene="lpdA"
                     /locus_tag="MB022_000134"
     CDS             56032..57438
                     /gene="lpdA"
                     /locus_tag="MB022_000134"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBT4465165.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57600..57875
                     /locus_tag="MB022_000135"
     CDS             57600..57875
                     /locus_tag="MB022_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBT4465166.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57881..58648
                     /locus_tag="MB022_000136"
     CDS             57881..58648
                     /locus_tag="MB022_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBT4465167.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58816..60645
                     /gene="typA"
                     /locus_tag="MB022_000137"
     CDS             58816..60645
                     /gene="typA"
                     /locus_tag="MB022_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBT4465168.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60906..62678
                     /gene="recQ"
                     /locus_tag="MB022_000138"
     CDS             60906..62678
                     /gene="recQ"
                     /locus_tag="MB022_000138"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBT4465169.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62792..63094
                     /locus_tag="MB022_000139"
     CDS             62792..63094
                     /locus_tag="MB022_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBT4465170.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63097..64260
                     /locus_tag="MB022_000140"
     CDS             63097..64260
                     /locus_tag="MB022_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4465171.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64286..67714
                     /locus_tag="MB022_000141"
     CDS             64286..67714
                     /locus_tag="MB022_000141"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBT4465172.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67735..68859
                     /locus_tag="MB022_000142"
     CDS             67735..68859
                     /locus_tag="MB022_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465173.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68849..69286
                     /locus_tag="MB022_000143"
     CDS             68849..69286
                     /locus_tag="MB022_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4465174.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69270..69608
                     /locus_tag="MB022_000144"
     CDS             69270..69608
                     /locus_tag="MB022_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBT4465175.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69727..70287
                     /gene="rsmD"
                     /locus_tag="MB022_000145"
     CDS             69727..70287
                     /gene="rsmD"
                     /locus_tag="MB022_000145"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBT4465176.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70284..70775
                     /gene="coaD"
                     /locus_tag="MB022_000146"
     CDS             70284..70775
                     /gene="coaD"
                     /locus_tag="MB022_000146"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBT4465177.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70765..71808
                     /locus_tag="MB022_000147"
     CDS             70765..71808
                     /locus_tag="MB022_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBT4465178.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            71967..72695
                     /locus_tag="MB022_000148"
     CDS             71967..72695
                     /locus_tag="MB022_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBT4465179.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72811..73056
                     /locus_tag="MB022_000149"
     CDS             72811..73056
                     /locus_tag="MB022_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4465180.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73053..73271
                     /locus_tag="MB022_000150"
     CDS             73053..73271
                     /locus_tag="MB022_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBT4465181.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73407..73946
                     /locus_tag="MB022_000151"
     CDS             73407..73946
                     /locus_tag="MB022_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBT4465182.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73939..76212
                     /locus_tag="MB022_000152"
     CDS             73939..76212
                     /locus_tag="MB022_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBT4465183.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76250..77296
                     /gene="holA"
                     /locus_tag="MB022_000153"
     CDS             76250..77296
                     /gene="holA"
                     /locus_tag="MB022_000153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBT4465184.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77385..77639)
                     /locus_tag="MB022_000154"
     CDS             complement(77385..77639)
                     /locus_tag="MB022_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBT4465185.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78039..78308)
                     /locus_tag="MB022_000155"
     CDS             complement(78039..78308)
                     /locus_tag="MB022_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465186.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78778..79233)
                     /locus_tag="MB022_000156"
     CDS             complement(78778..79233)
                     /locus_tag="MB022_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBT4465187.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79371..81299)
                     /locus_tag="MB022_000157"
     CDS             complement(79371..81299)
                     /locus_tag="MB022_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4465188.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81496..82329)
                     /locus_tag="MB022_000158"
     CDS             complement(81496..82329)
                     /locus_tag="MB022_000158"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBT4465189.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82521..83225)
                     /locus_tag="MB022_000159"
     CDS             complement(82521..83225)
                     /locus_tag="MB022_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4465190.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83248..83874)
                     /locus_tag="MB022_000160"
     CDS             complement(83248..83874)
                     /locus_tag="MB022_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4465191.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84200..86575
                     /locus_tag="MB022_000161"
     CDS             84200..86575
                     /locus_tag="MB022_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBT4465192.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86599..87090)
                     /locus_tag="MB022_000162"
     CDS             complement(86599..87090)
                     /locus_tag="MB022_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBT4465193.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87277..88197
                     /locus_tag="MB022_000163"
     CDS             87277..88197
                     /locus_tag="MB022_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4465194.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88351..88695
                     /locus_tag="MB022_000164"
     CDS             88351..88695
                     /locus_tag="MB022_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBT4465195.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88885..89385)
                     /locus_tag="MB022_000165"
     CDS             complement(88885..89385)
                     /locus_tag="MB022_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4465196.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89913..89996
                     /locus_tag="MB022_000166"
     tRNA            89913..89996
                     /locus_tag="MB022_000166"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89947..89949,aa:Leu,seq:cag)
     gene            complement(90320..>90754)
                     /locus_tag="MB022_000167"
     CDS             complement(90320..>90754)
                     /locus_tag="MB022_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBT4465197.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        29428 a        15657 c        19440 g        26229 t
ORIGIN      
        1 aattttcctt ctaacatata ggaaatgtca aaactattat gtgcgtatat tgactgcatt
       61 accgtttttc ttaaacctga ttatttattt tggtaataca atgggtcaaa gctttactta
      121 cgtcaatgga aataaaaggg caatgatttg gagtagtagt aacctgaata atctctttac
      181 agagttagga acgaaactgt cactttttta tccaggtact tggatagcta ctttagtagt
      241 cttggcatta ttactatgtt taattgttgg aatttattta agttttgaca ataagaaaac
      301 ttctattttt ttagttattt tgatgataat gggattttgt tcaaggttaa ttatgggatt
      361 ttctcccaca gtatgggctt ccggaatgcg gacgtactat atactttatg ttgttatcgc
      421 catcttagta ctaatggcag ttaaagagct aatgaaaagc atgagtgtac aaaaaaatga
      481 atttatgcaa tttggattaa ccgtgcttgg tatttgtacc tttataataa cggtgataaa
      541 cagataatat acaaaaaatc cctcgcttta gtattaggtg agggatttta ctcgtttttc
      601 gattaattgt tttaacactt gcaagtttct acgagtagct tgtttccgac cttcatatac
      661 tcacctcagg ctgtttacat gattgtacgt tctttttgta aaggatacag tgatatgtat
      721 ataatggcat aaagaaacgt tcaaacaaaa aaactcgtga agcaaaatta acttcacgag
      781 ttgtagtgca gatgatggga ctcgaaccca cacgagctaa cgctcacatg accctcaatc
      841 atgcttgtct gccaattcca acacatctgc gtaataataa cacactaatg atagcgaggt
      901 tttacatagt tgtcaagctc aaaaatggga ggacttatta agatggagaa atatgacgga
      961 gaattttcag gactgggaat gatcttaggt gtattgattg gacttgcatt cggacgtttt
     1021 ttgcttggtt ttatgctagg aatcatttgt ggaattgcaa tggattgggc agcgaatcta
     1081 tggaatgact atcatgacaa ttaagttctc aacgcttgtt tgtgtttttt taatacttac
     1141 gtttttccta aataagtggc aaaattttct aggatggagt atgatattga aatagtaaat
     1201 aacaaaaaat ttggaaagtt ctaacttttt gaaatgaggg attggctaag tgatgagaga
     1261 atttgaaaaa tccaacaaat tagaaggtgt cagttacgat gttcgcggtc ccgttctaga
     1321 agaagcggag cggatgcaag aagagggcat tagtatttta aaattgaaca cgggaaatcc
     1381 cgcacctttt ggatttgaag caccaaacga agtcattcgt gatttgatca tgaatgcccg
     1441 tgactcagaa ggctactcag attccaaggg gatcttttct gctagaaaag caattgagca
     1501 atattgccaa ctgaaacatt ttccgaatgt gacgatcaat gatatctaca ccggcaacgg
     1561 agtgagcgaa ttgatcacga tgtgtatgca ggggttgctg gacaatggag atgaagtact
     1621 ggttccaatg ccggactatc cgttgtggac ggcttctatt gcattagctg gaggcacacc
     1681 tgttcattat atttgcgatg aagaggcaga atggtatcct gatattgatg atatcaaatc
     1741 aaaaataacg agtcggacaa aagctattgt tatcattaat cctaataatc ctactggggc
     1801 actttatcca aaagaattat tagaagaaat cgttgaagta gctcgacaaa ataacctgat
     1861 tatttattcc gacgaaatct atgatcgctt agtgatggat ggtctagaac atattccgat
     1921 tgctacatta gcgcctgatt tgttcgttgt tacgctgaac ggtctatcca aatctcatcg
     1981 tgtagcagga tttcgagtag gctggatggt cctaagtgga gataagtcac acgtcaaagg
     2041 ctatatagaa gggctgaata tgttgtcctc catgcgtttg tgttccaatg tattgtctca
     2101 gcaaattatc caaaccgcgc taggcggtta tcaaagtgtc gatgagctgc tcctgccagg
     2161 cggaaggatc tatgaacaaa gagaatatat ctataatgca atcaatgata ttcctggtct
     2221 ttcagccgta aaaccaaaag ctgcttttta tatattccca aaaattgata caaagcgatt
     2281 caatattttg gatgatgaaa aattcgtttt ggacttttta catgaacatc atattctact
     2341 tgtacatggt ggtggattta attggaacca gccagatcat tttcgaatcg tttatcttcc
     2401 taaaatggat gatttgaaac aaacagctaa gaaaatgaga gaattcctag caacctatcg
     2461 ccaaaaatga ttttaaaaac caaatttttc agtaggaggg ccacagacaa acacactgag
     2521 gctctctttc tagtggagtt ttcttaaata actaaagtat attatttagt tgcaagtctt
     2581 ttcttgaagt ttgcgacttc ctaaagtaaa atacgaatag aatgcgaata cgcatatttt
     2641 ggggaggata cttatggaaa cgttaatcga gtcctttgga acgatcatta aagagattcg
     2701 aaaagaacaa aaaatgacgc aaaaaatgct ttcgcatgat atttgttcac aaagtgtatt
     2761 aagtcggatc gaaaataatg aggaactgcc gaatgtattg gtgatgatgc agatttgtca
     2821 acggctaggg gtaacgattg atcatgtgat gaatctctct cgaaaaacta ctcggacaaa
     2881 cgataaatta tttgaactgt tggatagttg tttccaaaaa cgtgattatc ggaagttaga
     2941 acaagtacta aaatcttctg atatttctga aaatctgtat caagcaactg actttcaacg
     3001 ctattattat tatctcggtg tttgcgaatt cacgttgcgt caaaatatct ctcagtccct
     3061 ttattatttg aaagaagcat taagctactc tagccaaaaa gaaagaatcc atgtttctga
     3121 cacagaaata caactgatta gttgtatcgg aaaaatatat ggtgtggcag gaaaaacggc
     3181 tgaagcacgc tactatttga gtcgaagcat ccaactgctg catcaatcag ctgatgaacg
     3241 atcaaaaagt gaattttcgc aaatttttta taattatgga aatttcttat tccatcaaaa
     3301 tgaaatcgac gaagcgttag aacaggtgaa tcaaggaatc tattgggctc aagagaagaa
     3361 tagctactat tacctcaatg atttatttgt gcttaaaagc ttaatataca agcgtaaaga
     3421 agaaccagaa agagcagcat tctatgaagg cttagcacaa gctgtgaaac aaatttcaaa
     3481 aaatttgtaa atggtgtaga ccattcttga cttcttctct aaataagagt actttagagg
     3541 tagaaaaaca aagggagaga atagaatgag aacttttatc tatgctgaca aattcttttt
     3601 agcgtcaggt gtcaaacatg caggctatct tgaaattacc gatggacttt ttggtgaata
     3661 caccaaagaa ctacctgagg gagaagtcaa catcattgac caaagcggca aatggattgc
     3721 tcctggtttg gtagatacac atattcacgg ctatatgaat catgatgtaa tggataatga
     3781 tgcagaaggc atcaaagtga tgtctgaagg tctgttatct tgtggagtga cttcattttt
     3841 accaacaaca ttgacgtcaa gcaaagaacg tttaaaagat gtagctgaga caatcggcaa
     3901 aatgtatcag gaagcaccag gagctaaaat caaaggaatc tattttgaag gtcctttctt
     3961 cacagaagaa cacaaagggg cacaaaaccc aagttatttc ggggatcctg atttggatac
     4021 tttccatgaa tggcaagaag cttcaggtgg tttaatcaaa aaaattgctt tagcaccgga
     4081 acgtcaaggt gtagaagagt ttgtcaaaca agtgactgaa gaaggagtag ttgtttctct
     4141 aggacacagt aatgctacac tagaagaagc gcaaaaagct gttgaagcag gcgcaagtgt
     4201 atttgtccat gctttcaatg gtatgcgtgg attgaatcat cgcgaaccag gtatggtggg
     4261 tgcgttatta tctttagaac atgtcttttc tgaattgatt tgtgatggcc atcatgtaca
     4321 tccaaatgct gcgggaatcc taatggaaaa agcaggacat gatcatgtcg cattaattac
     4381 agactgtatg atggccggtg ggatgcctga tggaaattac aatttaggcg aatttccagt
     4441 agtagtaaaa gaaggaacag cacgtctaga atctggcaac ttagctggaa gtatattgaa
     4501 attgaaagaa gcaattaaaa atgttgtaga ctggggatta gcaacaccag aacaagcagt
     4561 catgatggct agtctgattc cagcaatcag ctgtaaaatc gatgatacat gtggaaaaat
     4621 cgctcaagga cgcgatgcag actttatcgt attgaaccct gatatgacat tggaagcaac
     4681 ttatctagat ggcgtggaac gtttccacgc ataatataat ttaagttaac cttaaaacta
     4741 aacttcttaa gtttggtttt aaggtttttt ttgtgttcta gtaaaaatca ctttacataa
     4801 gtttcaaaag tttctttaat ttcctattgt tcaataagta acaatgtaaa gataaaatta
     4861 aaaatgttaa tacgtattag caaaataaaa gcttttttta ttgtgaataa atgaacataa
     4921 ttaggaggag aaagatgaac ttatttgaca tttttcgatc gactttgaca gatatgtcga
     4981 ttgtcagtgc aatcacttca acagttttta ttatattatt agggtttgtt tgtagaaaaa
     5041 aggggatttt ttctgaatct gttggaaaaa ttctatcgaa agtcgtctta acagtggctt
     5101 tgccagcact ggcatttcga gctttcatgc aagatatcaa tacagaatta ctgatgcaag
     5161 gaatcaatgt cttgatttgg ggattagtta tttatattat tcttattcta gtttcaaaac
     5221 cttttttctt aagctataaa ggggataagc aaacaacgtt gcgtgtcctg acgatctttg
     5281 gttctacgac ctttttcgga acgcctatcg tcagtgcgat ttatggtcct gtcggcgtga
     5341 tgtattcctc aattttcaat attggctacc gaatcttttt gtattcttat ggatacataa
     5401 aaatgagtgg cttgaagatg gaagctaaaa atatcaaagc gatgttcgtc aatccaatca
     5461 ttatcgctac ttttgccggc cttttcattt ggctgttcca agggtatctg ccacaagtgt
     5521 cagttacagg tgcagaagga gagataagcc aagtagcgtt tttgcgaatt gaccagactg
     5581 caccgtggct atttcagccg atggattatt tagctggttt aagttcacca ttggcgtggc
     5641 tagcaatcgg cgcaacatta ggtgaagtaa gcttcaaaaa agctgcatct gaaaaaactt
     5701 catggtatta cagtctagtc aaagtggtat ttgtacctgc attgaatatc gtgattctag
     5761 cagttttgac ggcaacgaat attctaccag tcaattttga agcactggca acaatcgtta
     5821 tcatgatggc tacaccagct gcaacagtgg cagcagccta tgctatcagt tttgataggg
     5881 aggcactttt agcctccaat gcatccttac tgtcaactat ttttgctgtg gtactgacac
     5941 ctgtatggat cgttgtatta caaatgattc atggcatagg tttattttca taagaaatgg
     6001 aggaagaaag tatgagaatc gcatgttacg gtgttcgtcc gaacgagata gatttttttg
     6061 aaaaattgaa tatttaccat tatgatctca gtctttatga agaactgttg acacatgaca
     6121 atatcgtgtc agcaaaggac catgatgctg tattgttgag gggaaattgt atagcagatg
     6181 agaccaatct tgcaaaaatg caggaatatg ggattcgtta tgtttttaca cggactgtgg
     6241 gagtcaatca tatcgatcta caagcggcag ctgattttgg tatgacagta gcccgtgtac
     6301 catcgtattc gccaaatgcg atagcagagt tatctttgac ttttgccatg atgcttttga
     6361 gaaatactgc gtatacgacg atccgaacat ctttcaaaga ttttcgagtg gatgagcaaa
     6421 tgtttagtcg tgaaatcaga aattgcacag tagggatcat aggaacaggc aggatagggt
     6481 tgacggaagc tgttttgttt aagggactag gagccaaagt gatcggctac gatattcatc
     6541 agacagaagc tgctaaaaaa gtattgacgt tccactcgct tgaagagtta ttagccaaaa
     6601 gcgatattgt cagtattcat gtacctcata tcccaggtga aaacgatcaa atgatcaatg
     6661 aaggattttt agctcaaatg aaaaaaggta gcatcttgat taatacagcc cgtggagagc
     6721 ttcaagataa tgaagctatt ttaagagctt tgaaaagcaa tcatttagct ggttttgcaa
     6781 ccgatgtttt tgcaaatgaa acagaggttt ttttccgatc cttcaagcca tgggagacga
     6841 ttccagaccc tgctatccag caattagtag aaatgtaccc tagagttctt gtaaccccgc
     6901 atgtaggttc aaatacagat gaggcattat caaatatgat ttcgacaagt ttcgagaact
     6961 ttcgtgaaat aatagaaact ggaaaaacga aaaatgaggt atctcttcct aaagcccgtc
     7021 aattaagata agaaaacatg gatattcgtc aataaaatgt ttaaaaatag tccaagtaaa
     7081 cagttgttac ttctgtatac ttggactatt ttgtatggga taattcgatg actcaacagt
     7141 aatctgtgag ctttttccgc ccattctttg tcaattcata acaggttagc ggccggccga
     7201 tttccaaata tttgattctt tcgttcaata tatcttgttc tactaaaaaa ttcaaatatt
     7261 tcttgacgga tatacgtgac agtgagcttt tttgagccag ttcttttgtt gaaaaaatag
     7321 tatcttcttt tagaataaat gcacttattt tttttaaagt cagttttgac agacctttag
     7381 gcaaatcgat gaatgatggt atttcgtgaa tgggagtggt gtggttgaat acttgatcga
     7441 tcaatttctg atcggctttt tcagtcgttt ggattgtctg agagaaagca agatactttt
     7501 cgatcgccag ctgaaaacgt tgcaaagtga acggtttgat caaataatct aaggcaccgt
     7561 agcggatcac attctttatg gtttcgatat catctgcagc agtgatcaaa atcactggga
     7621 tatcatttcc ttgctgtcta agccaagata aaaaatggat accactttcg ccaggcatgt
     7681 atacatcaag cagcagcaga tctgcgtttt tttgtctgaa atattttttt ccttcttcta
     7741 catttctgaa actttcgatt acgttcatat tagggatgct ttccacaaaa gtacgattca
     7801 attctgcaac cattggatca tcttcgatta tcaggacatt tgtcatcatt ttgatcttcc
     7861 ttttttggta attctagata gacgattgta ccgccatttg tttgtttgga aaattctaaa
     7921 aatccaccgt ggcgtttgac gattgaatgg atgagattca atccaaaccc acgattgatt
     7981 cccttcgtcg aaaaacctag cgtaaatatt ttttctgaga tttcttctga tatcccgaca
     8041 ccgtcatcgc agacggtcac gatgaatact tcagcttctg tgtcatagtt taaatgaatc
     8101 cggacctgtc cgtttctttc atgtttttgt agcacggctt cttttgcatt atcaagtaag
     8161 ttccctaaca cttgcaaaag tgaatggatg gagtcatcca tctccatttt agggataaaa
     8221 gaatcgcttt ctaacagcat ttcaacagat tgttctttgg cttcgtttat tttgcctaat
     8281 aaaaaaccgg caattgcagg tactttgatc agacttgaga cttggccaat ctctttttta
     8341 taattttgat ttagctgctg gataaaatgg gtgacttctt catacttctt caaatcaatc
     8401 aaaccagaaa tgacatgaag tttattcatg aattcatggg tttgagctct catggagttt
     8461 atatattgtt tcgtcccccc aagctcctcc attaatttca ttacttctga tcgatttcgt
     8521 aaagttgcga ctgctcctaa aaaacggtca ttgctaaaaa gaggtgtaac gctagaaata
     8581 gtttctatcc catttaaata catggattga gtccgaacgg tcgtttgatg ctcaatcgtc
     8641 tctttaaaca aaacgtcata tagattatca ctgatttttt tcctacttcg aaatccaata
     8701 gctgctctgt ttggtagaaa atgttttttg caggtgtatt gatcaagagg atagttttat
     8761 cagaagagat ggctaagatt ccttcatcta cttcattttc gataatcgtt ttttcactta
     8821 actgttctgc tatttcttgt ggttcaagtc caagcagtac ttttttcatt ttttgggcaa
     8881 gaaaaacggc gcctatgatg cctacgctaa gtccaataat caatgaaaaa acaatcattt
     8941 tttgtccatc tttgacttga ttgtcaatcg tgttgtttgt taagccaaca caaactgcac
     9001 caataatctc ttgatcataa taaataggga cgaaatagcg gaagccgaac cctaacattc
     9061 cttctttttc agagaaaaat tcttgtcctc caatcacttt tttcacatct tcatgacttg
     9121 agaaaggaag accgatgtaa cttgggtctg cgtgagaata tcgaatgcct tcagggtcca
     9181 tgacaacgat aaaatctaaa ttcgatgcaa tcatgatttc atttgttatt ccttgaatcg
     9241 catgtgtcgg gtcaccagtc tgcagctttt tttggatgag tttatcattg gcaacgattt
     9301 tagcaatgct agagatttta tctgacacac tagatcggtt catcgaaaga ataaaattcc
     9361 tgatcaatat ggcagaaaca atcaaagaaa cgaatgtgga gaaacaaacg acaaggatga
     9421 tcgtgttctg gagctttaat ggttttttct taaaaaaagg aaacataggc tacctcgaaa
     9481 gaatttattg tgaaaatttt aacatgaaaa caaaaattta acaaattgtc aacgataaac
     9541 acaaccttcc tataaaaaac accactgagt cggtaaaaga agctatacag aattttcaaa
     9601 cattggtatg atagacgtag aaatggaagg aggcgtgtat atgatagggg gaatcgcact
     9661 tgagacgatt tattggtata cattgctgat ttgtgcagga ttggcagtcc tattgattat
     9721 ttttggagat gtattcgatt ttgatggccc gcttgatccg atgcttatta tcccttggtt
     9781 aactttcaca agcttatttg gttttctagg tgagcgttta acagatcaat catctttatt
     9841 tttatttatt gctagtggag gcatatcgat agtgttggta tttttgctta atttctatat
     9901 attgatgccg atgaaacatg ccgaatcaac gatgtcgtct tctgaaaaaa ctctggaagg
     9961 acaagtagct gttgtgatca caccgatccc tgtacgaggt atgggagaaa ttcagttgaa
    10021 aagtgttacc ggttccatta gtcgtccagc ttgtttttac agcccccaag aacagacagc
    10081 tgagcgggga acgaaagtat tgatcattga gatcaaggaa agaatctgtt atgtcatgcc
    10141 ttatgaaagc atgataaaag cttaaaggag cgtgtgttaa atggaaatac cagttgtttt
    10201 tatcgcgatc gcaatcgcag ctttgatttt actgatgttg ctgatcgtct ttgtagcgaa
    10261 gtaccagaca gcaaaaccgg atgaagcgtt gatcatcagt ggaagttatt taggaagcaa
    10321 aaatgtgcac attgatgaaa gcaataacaa aatcaaaatt gtgcgtggag gtggcgcatt
    10381 cgtcttgcca gtttttcaac gttcgaatcg gatcagtcta ttatcgagta aattggacgt
    10441 ctccacccct gaagtgtata cggaacaagg cgtaccggtg atgtgtgatg gtacttcgat
    10501 catcaaaatc ggttcatctg tagaagagat tgctacagca gcagaacagt ttttaggcaa
    10561 gacaagagaa gaactggaaa atgaagcaag agaagtatta gaaggacact tgcgctcgat
    10621 tcttggttct atgactgtag aagaaatcta tcaaaatcgc gataagttca gtcaaagcgt
    10681 acaagaagtc gcttctgtag accttgcaaa aatggggttg atcatcgttt ctttcactat
    10741 caaagaggtc agagacaaaa acggttatct tgactcttta gggaaaccaa gaatcgccca
    10801 agtcaaaaga gatgcagata tagcagaagc ggaagctcta aaagaaacgc gtatcaaaaa
    10861 agctcaagca gaaaaagaat cacaagcagc tgaattacag cgacaaacag agatcgcaga
    10921 atcattaaaa gaaaaagaac tgaaattagc aacctacaaa caagaacaag atgttgctaa
    10981 agcaaaagca gaccaagcct acaatttaga aagtgcccga gcacaacaac aagtaatcga
    11041 acaggaaatg cagattaaaa tcgtagaacg tcaaaaacaa atcgaactgg aagaaaaaga
    11101 aatcacacgt cgtgaaaagc aatatgattc ggaagtgaag aaaaaagcag atgccgatcg
    11161 gtacgctaaa gaacaagaag cacaagctca aaaagtcaaa gaagtgactg aagccgaagc
    11221 agaacgattc cgtgtggaag cactggcaga agcggaagcc aataagacac gtttagctgg
    11281 acaagcagaa gccgaagcag cactagcaaa aggaaaagca gaagccgaag caaaacaaaa
    11341 gatcgccaat gccttcaagg aatatggcga agcagctgtc ttgagtatgg ttatcgatat
    11401 gctaccgcaa ttgatgcgcg aagcagcaca gccgctagga aatatcgaaa aaatctctgt
    11461 tgtcgataca ggaagcagtt caggagaaac cagtggagcg aaccgagtga caaattatgc
    11521 cacaaatttg ctttcttcta ctcaagaaac actaaaagcg acaacaggac ttgacttgaa
    11581 ggaaatcgta gagaatttgt cgacacgtgg gactactagc caagtaaata tgtatgaagc
    11641 gccaaaagag gcagaacaaa actcgatgaa ggaagaataa agtagtacac ttcaccgttc
    11701 aatcaaaaat ggaggaagac tttttaaagt tcttctctcc atttttttat tttattgcaa
    11761 aagaaaatta gtcctcgcta tttttctttt gtttttggat cttagcttct atctcttgta
    11821 aatttgttct tagcaatcgt tctttctttt ggagttcgac caaatcttct gatagttctt
    11881 ccttcttttc tgtttcagga ggacgaagaa agaaattgat accgattgca atgaatgtcc
    11941 ctataaaagt atctagtaca cgttggatag catacaaagc ggattcgcct tgagggatgc
    12001 tcaacgcaat cagcaacaac gtagctattg cggaaatgat accggaatta ttgttaatac
    12061 catctgacac gacgatcaca ataataacta gaataggaag caagatcaat tctaccaaaa
    12121 aatcattgtg aaaatagctt ttgacaaaaa aataaatgat agctaatgta ccacctaatg
    12181 tattgccgat gatcctagac tttccaaaag aaacggttgt agtcaagtcc tgacgcaaag
    12241 aaaaaacagc agataatgca gcaatcatcg ggattcctcg ttgagtcaaa tgaaaaaaca
    12301 agatacataa aaaaacagct aaggcacttt tgatcgtacg catacctagt cgaaaacggc
    12361 caaattgcat agagattctc ctttttggtc tttttactat catacctaaa cttttgtctg
    12421 atagctagtt tttcattatt caagtataat taaaataaaa aagggggaag taaatgcgta
    12481 taggaatgcg aacagtcaaa acagtgatca gtgtcttctt ttcgatggtc acagcgggta
    12541 gcttatcgct gttgtattgg ccagctgcag ggatcattgc attattaagt gtaggaaata
    12601 ccaagcgctc tacattgcgt acgggcatga atcgaatcgc agcctttata ttagctacgg
    12661 caacagcact gttcagtttt tttgctgttg ggtatactat ttttggcttc agtctttttt
    12721 tattcctgtt catcccgctt gcctctcgct tcaaattaac cgaagggata gtagtaaatt
    12781 cggttttggt cactcattat ttagtggagg aaaacatgtc ttggcagctg atcggcaatg
    12841 aagcgggatt aatggcgatt ggattagtat ttgctttgct tgcaaatgtt tacatgccag
    12901 atcgtacgaa gcagctgaag gaaaaccaaa tacagatcga aaaagaattt cgaaatattt
    12961 tgagacaaat ggcagaattt cttttatcag aaaataaaga agatgtgcag atcaaatgcg
    13021 aacatttact gacattcatt cgcgaaagcc aagaagatgc cagagaacat caagaaaact
    13081 actggctcag acagccgcta tactacgaaa cttacttctc tatgagaaga gcacaggcca
    13141 atgtgattaa agacatgctg gaaaatctag agagaatcca gcaaccagct tattatggaa
    13201 aacatatcta tggtttattg atttatactg cagaaacatt ttcagagtca aatgatggca
    13261 gacaaatttt aacacgtatt gaagaagttt atgtattgta tcggcaaatg cctctgccga
    13321 ctagtcgccc tgaattcgag gatcgagcag aattgttcca gtttttgcag tcttttaaaa
    13381 gttttattga aatcaaggca gaattctccc aacaaaaaat ccaaaaatag aggttgtgaa
    13441 ttcagctaat attcaggtca aaaagctgat ttcacaacct cttctttaaa cggtgttctc
    13501 ctgtcaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa
    13561 attgctcctt gtgactcgga gctgaacggt aggctcacaa cctctttctt aaacggtgtt
    13621 caaaagcttt cctctgcggt aataagtcca gccaaacgaa aaataggagg agctattttt
    13681 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctttt aaaattacat
    13741 atttttccat agaacttgca tttgatccaa gatttcgatg acatcttttg tcaagaagag
    13801 agagcgattg ggcaagtcac cttcaatcat gtgtaccata ttctttattt cgtaattcat
    13861 tgcttcagat gtattcccgc tttcgatgaa ttcctttgta ccatctgtaa ataagatctc
    13921 cgcacgatca gcacgcgggt aatcagcaat cgtcacataa ccgttctcaa aggcaacgat
    13981 accgactttt ggcatttttg cctgaaaagt caagctgaca gcagcaagtt cattttcttt
    14041 gttacgcaag atagtgacgg attgttcgtc cacgcctgtt tcaaaaggaa ccattgtaga
    14101 ggcaactact tcaggttgag agctcaaaaa gaaacgagcg aaagatacag catacgtacc
    14161 gatatccaac agagctcctc cagctaattc tggattgaag aaccggtttt tagggtctgg
    14221 ttctttgtaa ctgccgaaag gtgcttggat catttttaaa gcccccagtt ttcctgtatc
    14281 cattattgag cgtaactgct gataaagtgg catgttgaag attgtcatcg cttctgctaa
    14341 gatcaaattc ttttcttctg ctaagcgcat agcgtcagcc agttcttttt tattcatcgt
    14401 gatggctttt tcacatagaa cgtgtttgcc tgcttccaat gctgccaaga tatggtcgat
    14461 atgctgtcta tttggtacag caatgtacac gatgtcgatt tcagcatcgt ctaacatttc
    14521 ttgatagctt ccgtaagctt ttggaatatt gtaacggtga gcgaagtttt ccgctttttc
    14581 tgaagttctg gaagcaactg ctgttagttc gctcgtctct tgattgaagc tttcggcaaa
    14641 ctcatgtgcg atggtaccaa gaccaacgat tccccattga tattttttca tctgaaaacg
    14701 ctccttcata gttggattga gtttcttgtc tgtctattat tataacaaaa atcacgaaga
    14761 ataaagagga gaggacttga aacattaaaa aaaatgaaag cagcgattta agaggtgctt
    14821 tttgatcaaa acttaagtag aatagttaaa aggaggggat aaacgtgcat caagtatata
    14881 ttgatcttat tatgagtgta ttattagaac agtttgaaac tgaaaaaaat tttttagatg
    14941 aatacttgct gatcagtcca gaacaatggc aaaaatggaa agatggcgag aaaaatctgc
    15001 catctgaagc gatgcagaaa gtaaaaaatc ttttcactga ttatgaatgg atgctaacgc
    15061 aaaagatttt gcgtcaaacg attatttttc ccgaaaaacg aaacgttgcc gttgcagagt
    15121 acaagcaaat caaaacacgc atcgcgcaaa aatggcttag cagcaaagca gcaaaagtcg
    15181 agctgatccc tttgaaaaat aatcagcagg ctggtagata ccttgacttg aaagtaagta
    15241 ttcaatatga tgaatggggc tatgatgata ttttgaattt tcgtctgcca gcagatgttc
    15301 aaaaacagat cgaaggagaa aaaatcgaac ttctcgattg ggtgaatgaa aatctggaag
    15361 aaacttacgt tcatgaggcg aatggggaac gtgaagaaca acgaaaggat ttttcaagtg
    15421 aataaatttt taggatttgg agcagttgtg attatgatcg cagcaatggg ctatgttatt
    15481 tttagcgatg atgattcgca tgttcaagcc ggcaatcaag cagtcgaacg aacaagcacc
    15541 aaaacaactg ctagtaaaga aaagaacagt caaacaacca gcagcaaggt aaaggacaaa
    15601 gaaacaaaag atcgtacgtc caaacaattg ccagatgtta aaacagacga ttggaagcta
    15661 gttttggttg gtccagatca caaattagaa aaagaaattg atgagtctac tcaattggca
    15721 tcattaagta acggatactt gatcgacaaa cgcattgctc cagaatatga agcatttgcc
    15781 aaggctgctg aagcagctgg atttccatta gtgatggtct cagcttaccg gtctgtatca
    15841 tcccaacaac aagttttcga acaaaatgtc caagatgtga tgagtcgtca aggtgtatca
    15901 gaagaggagg cgacaaagat tactaaagaa acgattacgg agccaggata cagcgaacac
    15961 cacacaggtc tggcagtcga tgtcgtcgat caaaactggt acaacagtta tccttctact
    16021 gtattggatg caagttacgg agatcagcct ggagctaaat ggatcgcaga taatgctgcg
    16081 cagtatggat ttatcgttcg ttatccaaaa ggacacgaag atataactaa gatcacatat
    16141 gaaccttggc acttacgtta tgtcggtaaa gaaaacgctg agtatatcac caaacaccaa
    16201 ttgacgctag aagaatatct gaatgaatta aatgaaaagt aggaattgat catggcgaaa
    16261 aaaaagtgga aaaagcaaaa gcgcaagaat atccgttggc cagcagtggt tgccggtgta
    16321 gcagtgatac tcttagcctt tgttttttcc ttgaagaatc tgtcttctcc ttttactgat
    16381 aatcagcaag aaaatgaagc gcaatcccat cagcaattta ttgaacggct tgttccgcat
    16441 gcccaagaac tacaagatgg ttatggagtg cttcctagta tcattttagg acaagctata
    16501 ttagaatcaa attggggaaa aagtaccttg gctagcaaat acaataatct tttcgggatc
    16561 aaggcttatg gggatcagaa gaaagtaagc ctggaaacaa aggaatttgt taacgaagaa
    16621 tggataacta tccaaggaga cttcaaagtg tatgattcat gggaacagtc catggatgac
    16681 catacacaac tgttcgtcca aggagtagat tggaatcctg cgctgtatga aaaagtgatt
    16741 acggccacca actatcagga agccgcacaa gcattgcaag atgcaggtta tgcgacagac
    16801 cccggttatg cgcaaaaaat catccaggtc atcgagacgt atcaattgaa tcagtatgac
    16861 cggagatagc aaaggagaag tggctgatgg cagaacaatt tacgccagaa atcatgacgg
    16921 aattgagaaa agatatcgta agagtaccgg aaatcatttt agaagcaagc gggatcaaga
    16981 tatttggaaa attgatccgt tcgatcatct ttactacgga tattgcgatc atacgtaata
    17041 caaatgcgaa tgcagtaatt gctgtgtatc cttttacacc tcatccagcg attactaaaa
    17101 gcattattga ggcagctgac attccagttt tctccggtgt aggtggtggt ttgacacaag
    17161 gtgctcgttc ttcctacatg agcatgttcg cagaagcgca aggttcgtta ggggtcgttt
    17221 tgaacggtcc tacgccttta gagacagtaa aagaagtttg tcgagtagtt gatattccag
    17281 ttatcagtac ggtaacgtcg aaatatacag aaatagatga aaagttagca gctggtattt
    17341 cgattatcaa tatcagtgca ggtaaagaaa ctgcagaagc agtacgccat tttcgtaaaa
    17401 aatatcctga acttcctatc attgcgacag gtggtccaac ggaagaaagt attgctgaga
    17461 ccattcaagc aggagcaaat gccatcacat atacgccacc aagtagcgga gagcttttta
    17521 gtcgtaaaat ggaaaaatat cgggaaactg agaaaaagaa tcatgaataa caggtgagaa
    17581 tcaaaagacg aggaagagca gatagtggct cttcctcgtc ttttgtactc cagtactatt
    17641 tgttcagaat tcaaaaaaaa gagaaatttt tttgtttttt tacctgtaat gtacataaga
    17701 agagttaaaa ccttttgtta ttattagcat tctaacaaaa aaatgtcttg atttattgaa
    17761 gcgctttcta tagaatgaaa aacgaataaa aactaaaaaa ggcacccgac aatttagtag
    17821 cagctaaatt gtcggggctt gaaaagtcag cgggaactga ccaatcaagt tgccatagtc
    17881 aatgcttgtg catcgacgtg tatattgtac ctaatttcat acagaattac tagttatttt
    17941 tctgtttttt agggaagagc gcacacgaag aagcatgctt atttagctat gtcaaggata
    18001 ttgggcagtt gtctgatatc cttttttctt tgcgtaatta tcctcccatc attgaagcaa
    18061 gaagtgtttt tagcgatgaa agaagaatca gcactatcta atttttcttg aaagtattga
    18121 gttgttcatc ctaggcagaa cgataaggca agtcttggtt tttattcccc ttgaaaaaga
    18181 tcgtttaata aatatgttct gtataaaaat acagtatatt gaaaaataac aatggaagta
    18241 gaggtgcaga gaatgaagaa aagaatgaga atcatcttag ctttaagtgt tatgttattc
    18301 ttcttgacgg cttgtgggca agaagatgac aaaaaaatca cgctgaacat ggcttggtgg
    18361 ggttcgcaag tccgacacga tgcaacagta aaagtaatcg agctttatga agaaaaaaat
    18421 ccccatgtga cgatcgatta tgaattttat gattatgaag gatattacac aaaattaggt
    18481 acactagcag catcagatga tatctgggac attttccaac tgaacaatca atttcctcaa
    18541 tacatcaatc agattgaacc attagatgaa tatgtggagc aagggataat tgatatttct
    18601 aatgcagatg aatcctattt agcaacgacg acttacgacg ggcaattagt tggactgtct
    18661 aacggggtga attcgtttgc gattgcttac aataaaaata tatttgaaca attaaatatc
    18721 cctgaaccag cttcaaattg gacatgggat gaatttgaac aaatcagcta tcaaatcact
    18781 gaagaattag gaatctatgc tatttcaaga ttcgaagatt ttatagctgg ctgtattatt
    18841 caaataccac aagttgagaa aggattgaat ttttataatc gggaagatct atctaacagc
    18901 ttgggatttg aaaatccaga ctatctaaca gattttttcc agctgcgtaa aaatctggta
    18961 gacaaagggg cccatccaga accaggtggt ggagctgcaa gtacgacgga tctgtctttt
    19021 caggcggtta gggatagaga agcggcaatg gtgtttctat caagtaacca gttgacagaa
    19081 atattgtcag gggctcctga agatatggag attcagttga ttaatccacc tcgcagaaaa
    19141 gcagatggag aatctggtat ccctttacgt tcttcgcaaa tgctgagtat ggcaaaaagc
    19201 tcagcccata aagaagaagc tgctaagttt attgatttct ttcaaaacag tgaagaagcc
    19261 aatgaaattt taaaaggaga acgtggtgta ccaatcatgt cgaatattcg agaacagctc
    19321 gctcgtgcag atgatgcaac tttgcaagcg acatttgatt atttagaaat gatttccgat
    19381 atggacaacg gcgaagtgaa tgtctttgat agtgctttga atccggaaat tcaagaccaa
    19441 tacaatcttt tagttgaaaa agtcatttat gaagaagtaa cagcagaaga agcagcagag
    19501 agtctttatg agtttgcgtc aaacattcta ggatagcagc aggaggaagg catgaaaaaa
    19561 gaaaaactaa aacggttctt ggatcaagac aatgtggtcg gttacgtatt tgcttctcca
    19621 ttcatcctag gatttctatt gataacagtg ataccgatga tcttgtcact ttattattcg
    19681 ttcaccgatt atcgtttggg aaatccagtc tcatggatcg gcttggataa ttatataaag
    19741 atgctgagtg atgcccgttt tttgaactcc atccgagtca cttttctcta tgtatttgca
    19801 tctgtacctg tgaaactgat ctttggtcta ttgattgctt atcttttaac gaggaatatc
    19861 aaaggaacga ctttctttcg ttctttgtat tatgtgccta gtttgatcgg tggcagtatt
    19921 tctatcgctc tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa tattttcctg
    19981 gaacgattag gcgcacctgc gctttcctgg tttagcgatc aaaaactggt catgatccct
    20041 cttgttttga tgtctgcttg gcaattcggt tcggcaatga tcatctttgc tgcaggtatc
    20101 aaagagatcc caggctcgta ttatgaagcg gcagagatcg atggagcgaa caaatggcag
    20161 acgttttaca agatcacgtt acctcttctt tcgccgatca ttttgtataa cttggttatg
    20221 cagacaatcg cggcgtttat gacttttacg caggcattta tcattaccca aggtggtccg
    20281 aatgatgcca cgaactttta tgctctttat gtgtataacc aagctttttc ttttaattct
    20341 atggggtatg ctagtgctat ttcgtggatc atgctgttga ttatgggagc tgtcaccttt
    20401 ctgattttca aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa
    20461 taataccgaa atggtttcaa tacggtttta ttttgctatt aggtctagtc atgatttatc
    20521 cggtcttatg gatgatcgca ggttctttta aaacggatca agagattatg agtggttcgc
    20581 tgaatttgat tccagagact tttcaatggg aaaattacgc aaaaggatgg gcggggtttg
    20641 ctggaatttc attttttact ttctttagga attcgtttgt catctctgca gtttcgacga
    20701 tagggacagt tctgagctcg acttgtattg cttacgcatt atcgagaatc gatttcaaag
    20761 gaaaaagatt ctggttcgtc gtcatgttag taacgatgat gattcctgca caagtgattt
    20821 tgattccgca gtttattatt tacaatcggc tgaatttggt tgggacgtat gtgccgctga
    20881 ttttaccaca tttttttgga caagcatttt tcatttacca gatcatgcaa ttcatggtca
    20941 gcatccccaa agagttagat gaatcagcga ttattgacgg atgcagcaag tatagtgtct
    21001 ttaccaaaat tatttttcct ttgctgaaac cttcgatcat cacgacgatc atcatccagt
    21061 tttattggaa atgggacgac ttcatgggac cattgattta tttaaacaag ccgagatctt
    21121 acacggtatc gattgccatc aaattatttg cagacgctgg atctagtacg acttacgcga
    21181 gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt tctgattttc ttattcttca
    21241 ataaatatct tgtccagggg atcagtacaa gcggtctgaa gggctgaata tgaaaaagcc
    21301 aatagaagag atgtttcttt acaagctcaa ctactatgtc tgtcggattt ttttaaccag
    21361 tcttatcagt aatgctcttt tcgctttagt tctaggatgt ttgttcactt taggattagt
    21421 gctgtctgct cgtgttgtct atctgattta ttttttgatg atgtatcctt cttattatct
    21481 atctgtccat tacttggcag aaaaaaaggt atgttggagg aaaggaaaaa tcaaacggat
    21541 gtgtttcatc attacgttgt ttcagctgtt tgtcattatt tttgcagttg attggtattt
    21601 attagtggga aagattggag cttggtatct gattcctttt ctattgtgtc tgttgctttt
    21661 tgtatcccat agttttttta gtacgctaat tgatatctgc aaagaagata aagcagatag
    21721 tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca aaaaacgtca aggggtttct
    21781 gtggtattta tgccttttgt atggactgtt ctggtgtata cagcatgatt ttttactcac
    21841 tgcattactg tatccagggc tgccatttgt cacagtattt tgcttaaaag aaaaggagaa
    21901 tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg gtgggagagc gcgttttttt
    21961 tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac tagtcggttt ttgtgatgta
    22021 tctcaaacaa ggatggatta tgcaaatcgg atacttgtga acaagtatgg acagaaaaaa
    22081 gtaaagacat gtatgccatc acaatttgag caactactgg atgacgtacg gccggctgaa
    22141 gtcattatta cgtcagtaga tcgaacgcat catgattata tcattcgtgc aatgaaaaaa
    22201 ggatatgatg ttatttgcga aaaacctctg actatcgatg aggaaaaagc tcaagaaata
    22261 ataacggtcc aaaaacagac gggaagaagc ctgcgagtca cttttaatta tcgttatgca
    22321 ccgtatcaca cgaagataaa ggaactgata gaaagtgatg tgattggtga tgtgttttct
    22381 atccacttcg aatggctgtt aaatacgcaa cacggggcag attatttcag aagatggcat
    22441 cgaaataagc acaatagcgg cgggcttctt gttcataaat ctacccatca ttttgatcta
    22501 gtgaattttt ggttgaacag ccgtccccaa aaggtatttg cattcggtgg attaaacttt
    22561 tatgggatgc ataatgcgga gaaacgcgga gtagaaatat tttacagtcg atgctatgga
    22621 agcgaactag ctaaagctga tccatttgct atagacctgt cagccaatga ggaattgcga
    22681 gaaatgtatt tagaagcaga aaaagacagt ggatatattc gtgaccgcag tgtattttcc
    22741 gatgatatct ctattgaaga tacactaggc gtaacggtca aatatgaaac tggtgcgatt
    22801 ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat tcaatgtttc tttcaatggc
    22861 agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt acatcaatgc aggtggagat
    22921 aaaaaggatg aaggagcagc cgttcttcag tccatccatg tctatccgat gttcggtgag
    22981 ccatatgagg taatcgtcga acaagaagcg ggcggacatg gcggaggaga tccgcgttta
    23041 ttagatgatc tatttggtga agaaaaagaa gaagatccgt ggaatcgggc agcaactcac
    23101 gtagatggta ttttgtcgat tttgacagga atcgcagcaa atcattctat tgcttcagga
    23161 aaagcagtag tgattgacga gcttgtcagc ttaaaagaat aagaatagat ttacggaaat
    23221 tacttcccgt agtttataaa atcttgcctt attgccgata ggctgtcttt tgaacactat
    23281 ttattcggaa aaaggggcta tgacatgctt tttgtcatgc cttcttttta tgtgggctgc
    23341 ttttttagat aggagaaaat aaaaataact atttaaaccc gaacaaaaag gaataatgca
    23401 ttaaaaatca ttagatttta caaaaaaata agtaaaccac gttgtcagtt tcagaaagta
    23461 gtgttagact gttttcaata tttgaaacaa cattttttat tcaaatatgg caaactgcct
    23521 tcacgtgcaa aacagttgca agaaaatgaa tttacactat tattagtttt acatacctac
    23581 ttatatatcg tcataatatg gatgacagtt tctagccagt accgtaaatg ctggactata
    23641 agtaaaagat tggctatttc acccttcttt tcttatgata aagtggaggg tgtttttctt
    23701 taacagaaac atagtaggtt tttgcacaaa agcggttatt tcggtattaa taaatgataa
    23761 aggagcaaat aaagtgaaag aattagttga acgtattcaa aaagacggac gtatattagg
    23821 cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa gtagaccctg tattgatgga
    23881 acaaatggga aaacggtttg cagaagtatt taaagaacaa ggaatcacga aagttgtgac
    23941 cattgaagct tctgggatcg caccagcttt atttgctgca agagaattag atgttccaat
    24001 gatcttcgct agaaaagcaa aaagtttgac gatggatgaa gaattattga cagcttctgt
    24061 ttattctttt acgaaacaag tgacaagtac gatttctatt tctcgtaaat ttttatcttc
    24121 agaagacaaa gtattgatta tcgacgattt cttggcaaac ggacaagcgg caaaaggatt
    24181 gatcgaatta tgccagcaag ctggagcgca agtagaagga atcgggatcg tcatcgaaaa
    24241 gtctttccaa gatggacgtc agttacttga agaaatggga ttgagagtcg tttctcttgc
    24301 gcgtattgct tccttgacga atggacaagt tgagtttctt gaggaggacg cataatcgtg
    24361 gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc tgcagcatct gctagcgatg
    24421 tatgcaggag cagtagcggt gcctcttttg atcggtacag gactaggctt taatcaagaa
    24481 cagatgactt atttgatttc aattgatatc tttatgtgtg gattagcgac attattgcaa
    24541 ttgacagtta accgattttt cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa
    24601 gcagtagctc cactgatctt gatcggaacc aatgaaggag taggagctat ctacggatct
    24661 attatcgctt ctgggatctt tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa
    24721 ctattccctc ctatagtaac aggaacggtt atcaccgtta ttggtttgac tttgattcct
    24781 gtagcaatcg agaaaatggg tggaggtaat gcaacagcag ctgattttgg tgacaaaaca
    24841 aatctgctac ttgcatttgt gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa
    24901 gggttcattc gttcaatcgc ggtattgatt ggtttagtcg gaggaagcat cttagctgct
    24961 ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa
    25021 cccttttatt ttggtaaacc aactttcgac gtatggtcga ttttactaat gatcatcatt
    25081 tccatggtca gcatggtaga atcaacaggt gtatattttg cacttggtga cattactggg
    25141 aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg cagaaggact tgccgttatc
    25201 ttaggcggta ttttcaatac attcccttat actggctttt ctcaaaatgt cggattggtt
    25261 caactttcgg gaatcaaaac aagaaaaccc atctatttct ctgcattctt cttgattgtt
    25321 ttaggattat tccctaaaat cggtgcgtta gcacaaatca tccctgagcc ggtacttggt
    25381 ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag gaatccgtat gctgttggaa
    25441 gttgatttca gcaatgataa aaatctattg attgttgctg tttctgttgg atttgggctg
    25501 ggtttcaatg ttacgccgac attattcagt caaatgccgc aaacattgca aatgttcact
    25561 ggaaatggca tcgtaatgag tagcattacg gctatcatat tgaatttgat ctttaatgga
    25621 ttgaaaaaag atgaagaaat attggaagaa gccttcaaga agtaatagag caaaagaaag
    25681 gttgtgaaat cagcgttttg acctgattgt tagagaaaca aacggaaaaa tagttcctcg
    25741 tattttgccg tttgtttgag ctaataccga gggtcagctg attgaacacc gttgaaggaa
    25801 gaggtcgtga gcctgccgtt catctccgag tcacaaggaa caatttcaaa aaaaagcttc
    25861 tcatgtttgt tgaatattgt tccttgtgac cgaggagttg gcaggagaac accgtttatc
    25921 cataaaaagg aatgtgacct aacttttgtc acattccttt tttttatctt taataaacac
    25981 gaacaataaa atgtttttct ttgttaacat tcgaaatttc tttgacatca aaaaaagtga
    26041 ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca atgacagcaa aaatctctgt
    26101 gattatgggg agtacttcag attgggaaac aatgaaagaa gcatgtcaaa tattggatga
    26161 attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt
    26221 tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc attattgcag gagcaggggg
    26281 agccgcgcat ctgccgggta tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc
    26341 ggtccaatca cgaacattaa atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg
    26401 aggagtacct gtagctacta cagcaatcgg aaaagcggga gcaattaatg ctggactact
    26461 tgcgatacag atgctttcca tgtatgattt agaattggag gctaaattgg ctgaaagacg
    26521 ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa cttggttaaa cctttgttgc
    26581 ctggttcaac gatcgggatc gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg
    26641 caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac
    26701 aagttgcaga ttggcacatt gaaagtgtct atgatgacac ctttgcgctg aaagagcttg
    26761 ccagaagaac agatgtcatt acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg
    26821 cgattttacc catgtcattt attcctcaag ggacagactt gcttgcgatt acgcaagacc
    26881 gcctgttaga aaaatctttt ttggaaacga ataacatcgt gattgctcct tatgcaacga
    26941 tcgtcagtcc gacggatatc caagatgcga ttgacgggat cggctatcct tgtgttttga
    27001 aaaccactcg aggcggctat gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc
    27061 tagctcctgc catggacctt ttaagagaag gaacctgtgt tttagaagca tggatcccgt
    27121 ttgaaaaaga gatttcgatc atggtggcag gaaatggtca aggagatttc acgacttttc
    27181 cggtcgtgga aaacatccac cacaacaata ttcttcatga aacgattgct ccagccgcga
    27241 ttgatcagga tgtgatcgag gaggctgagc gtattgcccg tgtgattgca gaagcagtga
    27301 gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga
    27361 atgaattagc tcctcgtcct cacaattctg gacattattc tattgaagct tgttctatga
    27421 gtcagttcga tgcccatatt cgaggtattt gtggttggcc attaggagaa aaagtacgtt
    27481 tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca actgctggaa agctatcgct
    27541 tgattgagaa taagccagac tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag
    27601 gtcgcaagat gggacatatc actatcttga cagaagacat ccagcaaacg ctggaagaaa
    27661 tcaaacaaac gaatatttgg gattagagga gaaaaaacat gttagaacgt tatacacgcc
    27721 ctgaaatggg gaatatctgg acggaagaga acaaataccg tgcgtggctc gaagtcgaga
    27781 ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa
    27841 ttcgtgagca tgcatcattt gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc
    27901 atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt gggagaagaa agaaaatggg
    27961 tccattatgg tctaacaagt acggatgtcg tagatactgc ttatggctat ctcatcaagc
    28021 aagcaaatga tattttacgg gaagacttga aacgttttac ggaaatcatt ggcgaaaaag
    28081 caaaagaaca caaatataca gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa
    28141 ctacttttgg attgaaactt gccacatggt attcagaaat gaaacggaac atcgaacgat
    28201 ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg
    28261 caaatatccc gccatttatt gaagagtatg tatgtaaaca attggggatc cgtccacaag
    28321 aaatttctac tcaagttctc cctcgggatc tacatgctga atatttctca gcgatggcgc
    28381 ttattgctac aagtattgaa cgttttgcaa cagagatacg cggattgcaa aaatccgaaa
    28441 cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca
    28501 aaaggaatcc gatcggttca gaaaatatgg ctggtctagc cagagtggtg cgcggacaca
    28561 tggtcactgc ctatgaaaat gtcacattat ggcatgaaag agatatttct cattcttcag
    28621 cagaacgaat catcttgcct gataccacga ttttattgaa ttacatgtta aatcgttttg
    28681 gcaatatcgt taaaaatcta acggtctttc ctgaaaacat gaagcggaat atgtctgcaa
    28741 catttggttt gatttatagc cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa
    28801 gagaagaagc ttatgatctt gttcagccaa aaactgcgta cgcttgggac aaccaagtgc
    28861 agttccgccc gctgttagaa gaagatccaa ccatcacatc ggtattatct aaagaagaaa
    28921 tagacgatgc ctttgattat catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag
    28981 taggtttgga ataaagaaga gtgagttgca aaattcaatt aaaatagaat agcaaaaata
    29041 accaaaaaag aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta
    29101 tcaataatag gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca
    29161 aaaaatagag gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga
    29221 gatgttgcag caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct
    29281 ggatacgata atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc
    29341 ctttgtgttc tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg
    29401 ctcggcggat ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt
    29461 tgatcgacag caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa
    29521 aaagctgacc ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc
    29581 aaatttttag ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg
    29641 tataagattt tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta
    29701 catatttttg ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa
    29761 aaaacgatgt atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga
    29821 acttttgtat gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg
    29881 gatagaatat ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg
    29941 aaaaggagaa ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg
    30001 aataaaaaat cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca
    30061 aattatccct ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct
    30121 aggtgttccc gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga
    30181 ccagttggat gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc
    30241 ggaagaaatc agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt
    30301 atttgctaag gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg
    30361 acaaattttg cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac
    30421 gaatgaacat atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta
    30481 tcaagaagta tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat
    30541 ttcgtaaaag tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta
    30601 aaaaaagcag tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac
    30661 tttgagatac aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt
    30721 gacaagctgc ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag
    30781 gaagtataac atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat
    30841 gttatgggca gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc
    30901 ctctttagaa ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt
    30961 gagatgcggt gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga
    31021 agaagggaaa ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact
    31081 tttgccaggt gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt
    31141 gaaagtcaac cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct
    31201 tccagttgct catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa
    31261 aaaaaataat cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat
    31321 tgcagggatt acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc
    31381 agtagaagac ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa
    31441 cttcggaaag gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa
    31501 gaaaaaaaga atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga
    31561 agatatctta ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag
    31621 cgaacattgt tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc
    31681 tcatgtgttg caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc
    31741 agttgtcttc aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc
    31801 tgcgacagga gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc
    31861 gttgctagat tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga
    31921 agaagtagtt gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg
    31981 cgaaattgca tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg
    32041 tttgattgaa cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat
    32101 gtatgtaggt gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga
    32161 atttaatcaa gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga
    32221 aaagctgctg cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg
    32281 tatacaggat atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc
    32341 tggctcagga cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc
    32401 ttatgaaatg atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga
    32461 ggaagaaaaa atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa
    32521 agtaacagac gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc
    32581 agtcgatgca ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg
    32641 aatcgtagaa tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt
    32701 attgcttgat ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta
    32761 tgattcacaa gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag
    32821 ggttagaggg acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta
    32881 tttggatcca aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc
    32941 tagcggtggt aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc
    33001 agaagttttt tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt
    33061 aggaactcct gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat
    33121 ctatccaaca ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac
    33181 tcaggaattc aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt
    33241 tgatggcagt gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt
    33301 tgatctatcc attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt
    33361 gattgaaagt gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc
    33421 tttcaaacat cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc
    33481 agaaactcaa tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga
    33541 aatgatggga gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat
    33601 ttcagcaact gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga
    33661 tgcaatcgta tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt
    33721 tggggtcatc cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga
    33781 ggacaagaag gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat
    33841 ttggggctgc tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa
    33901 gctgcaatcg gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag
    33961 ccttttctat tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc
    34021 aatgctcgtt cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc
    34081 gatacggaga tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa
    34141 ttaaaaagtg ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg
    34201 gcaatgattg ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa
    34261 aacggagcat acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt
    34321 ataagagatg tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat
    34381 tgctttacga aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga
    34441 ccagattcaa acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat
    34501 ttagcgaaag aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg
    34561 tcagccgcaa gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa
    34621 aatcaataca ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt
    34681 cgaatgaaat tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat
    34741 gattcaattg ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg
    34801 gcaaaagaag tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg
    34861 attgatattc aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa
    34921 gaatgtatcg gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc
    34981 ggtctggaaa aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat
    35041 ccgacgccgt tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg
    35101 ttcttttaaa agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa
    35161 agctggcgta gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa
    35221 aaaaacggaa cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt
    35281 atcggtcttt caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa
    35341 attagcagtg gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat
    35401 gtgtgtcaat gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc
    35461 aacggggaaa aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg
    35521 tgttcaagga aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc
    35581 aggtaatgat tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt
    35641 gacaggagaa acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca
    35701 ttcaaatggg tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc
    35761 atcaaaactg ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat
    35821 ctatgtttct tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat
    35881 cactggtgga gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca
    35941 aatcaaactt ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga
    36001 aattcctgag atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt
    36061 cgaagaatcg aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta
    36121 tgaaatcggg aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg
    36181 atgagaatcg ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat
    36241 ctatcgaaaa aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca
    36301 tatgttttga aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa
    36361 tttgattcaa aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc
    36421 gatttgatcg tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat
    36481 gataaaagaa tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc
    36541 atacgtgatg cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac
    36601 caaggtgtag atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac
    36661 acgttcgatt ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg
    36721 attagtgaaa ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat
    36781 cagataaaac tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca
    36841 tttcaacagg aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg
    36901 aagaagtgac agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa
    36961 tccatggtgg tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg
    37021 aaaatatcca accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta
    37081 tgaaaccaga tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta
    37141 tgctccgtag tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact
    37201 atgaagaagt actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa
    37261 aactagcggc aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact
    37321 taacggattg ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac
    37381 aaacgcttcg ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc
    37441 tggaaaaata ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata
    37501 atatccgaga tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag
    37561 tcgccttgaa acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt
    37621 gggaagcagc gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc
    37681 gagaagtaga tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg
    37741 ccccttctta tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac
    37801 tgacagtaga ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag
    37861 gtggacttct tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg
    37921 tgacaaaacg taagccgact ccagcagaag caaaagcatt agtgtttgct tggagagctg
    37981 tcaaacatgt gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg
    38041 gagccgggca gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga
    38101 aaatggaagg tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat
    38161 atgcagccaa acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag
    38221 attctattga tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt
    38281 ttagacatta gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg
    38341 aacatgcaat aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc
    38401 caggaaatcc ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc
    38461 attcagcgtt aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg
    38521 aagttccttt gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg
    38581 gtccgacaaa agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg
    38641 atacttatca aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag
    38701 cgtatatcaa agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga
    38761 aaggtgtagt cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga
    38821 accagcatcg atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag
    38881 aagaattctc ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc
    38941 aagatcataa gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt
    39001 attcaccagt accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa
    39061 aaccagcggc tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg
    39121 gtttgattgc tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc
    39181 ctgaaacgca agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt
    39241 tattagagaa tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg
    39301 tggttgcagc tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc
    39361 tggaagaaga gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg
    39421 gagcaggagg acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga
    39481 aaaaaattta caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata
    39541 ttggagaaaa agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat
    39601 aaaaaataca acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt
    39661 gtggtacact tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta
    39721 ggtacttctg atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg
    39781 gaagatccag taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat
    39841 gcagatattt atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca
    39901 tcaatcaaac gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg
    39961 tttgattttt ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa
    40021 atggattatg tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa
    40081 gtagctgaag cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc
    40141 aatcaaaaac caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct
    40201 aatcagctgc aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat
    40261 atgacagatg aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat
    40321 gatatgacga tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc
    40381 gggaacgaaa aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa
    40441 acgacaccaa ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg
    40501 atcgccggta cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat
    40561 atgtctcggg aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga
    40621 ttgtcataag caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt
    40681 ttcagcttcg agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga
    40741 tttcgtttat tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga
    40801 aatcccttgt tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc
    40861 aaaagcaata tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat
    40921 tctttgttct gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact
    40981 gaaatgagag ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc
    41041 ggtaaaattt tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct
    41101 tgaaggctgt gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta
    41161 aagcgagtcg tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc
    41221 ttgcgggatt ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa
    41281 acgatataat cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt
    41341 agatacttcg tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga
    41401 atcaactgga agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag
    41461 ggaactggcg gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat
    41521 tctttctgcg acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc
    41581 atggaccaac gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc
    41641 aactattaaa aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc
    41701 cctaaataaa aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac
    41761 ttggtttgct gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc
    41821 agcaagtttc aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg
    41881 attttttttc aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc
    41941 taaagcagtt actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag
    42001 cagataatca tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc
    42061 actaataaat atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa
    42121 cccgtccatg aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat
    42181 tgaagacagt gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga
    42241 accaaggaca gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat
    42301 aaaaacttta tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg
    42361 tgacagttgt tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt
    42421 aataaagaag gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat
    42481 cgatcccttt ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc
    42541 cgttgtctat aactttgaat tggatcatct catcaatttt gcatgtggta atacgaataa
    42601 atcctttgtg acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca
    42661 agatcatttt taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa
    42721 gcatatggcc gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt
    42781 tttcaagagg aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg
    42841 catgggtcat agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat
    42901 ccaaactgtt ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt
    42961 gtgctaaatg gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca
    43021 ttttattcat gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa
    43081 agccaaggtt ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt
    43141 ggacctttct ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa
    43201 ctccaagttc cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc
    43261 ttgctaagtc gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga
    43321 cagaatctac ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat
    43381 tattgatcag ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta
    43441 acgtactaat ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa
    43501 gttcttctag gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt
    43561 cccacatact ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga
    43621 tatttgcgat cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta
    43681 gcaaagggat aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta
    43741 tgcttaaatt ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct
    43801 atattctgct gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct
    43861 gtttgaatat ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt
    43921 ttacactcga ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt
    43981 ccatcaatag ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca
    44041 gctaaagcac cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa
    44101 tcatttgttt ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg
    44161 acaattcggt gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg
    44221 gttggtttac gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag
    44281 ttctctgaac cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga
    44341 ttgctgacaa ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg
    44401 acgaaaagct cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct
    44461 gctcgctttg cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg
    44521 atcatatcca ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc
    44581 atgtagccat ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat
    44641 tcagtatgct ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt
    44701 gggtcaggtg cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca
    44761 agggctaaaa aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac
    44821 tatcttatca gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag
    44881 aaacttaaaa aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata
    44941 aaaaaatcaa tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg
    45001 gataaaaaat gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa
    45061 gcattctatt aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag
    45121 gaatcgctat gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct
    45181 cggaattaga aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa
    45241 atcaagtagc acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt
    45301 ctatcgatgg ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg
    45361 tgatcgccta tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg
    45421 ataattttgg agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag
    45481 aaggaaaagg accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac
    45541 taatcaatta caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg
    45601 tcgtgccatc tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta
    45661 cggcgcaagc aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg
    45721 ctgttttgtc gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg
    45781 ggtatgggag tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg
    45841 gcgttaaatc gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct
    45901 tagcaaaaaa tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca
    45961 atgatactga aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag
    46021 tatctgtcga taaagataat tatcaagcag agctgatcga caccgattat tataaagaat
    46081 ccgatctgaa aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag
    46141 gataagaatt atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg
    46201 aatccgagcg ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg
    46261 cggagaaaac ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata
    46321 tggctcctat gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa
    46381 agatagtgaa gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct
    46441 ttcaataaaa gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg
    46501 ggaactgaca gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc
    46561 gaatcttcta gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc
    46621 attatcaaaa tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga
    46681 agaaagcgcg aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat
    46741 cattatttca cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg
    46801 ggatggacag acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg
    46861 cggaatggtg ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga
    46921 agtttatttt aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat
    46981 aaacgaccac atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat
    47041 gggagcgggc agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat
    47101 atccggacgt atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat
    47161 tgcaaacgga ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat
    47221 ggatattcgg gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt
    47281 agataaagaa aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac
    47341 aaactccatc catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt
    47401 atccaagtgg ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat
    47461 agatgttggg gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa
    47521 agctgtctgt cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta
    47581 tacgatgaac gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt
    47641 cttgatgaaa ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta
    47701 cggataaaaa agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct
    47761 acagcatggt gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta
    47821 ttttctggcg tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac
    47881 tagctgcagg gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag
    47941 tgatggcgtt tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt
    48001 ggatgacagt tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt
    48061 gggtagctta tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc
    48121 gtggactgac acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc
    48181 aaaaaatgtc tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga
    48241 cattgatttt aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa
    48301 atcgtcggaa aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg
    48361 tatttacagc agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc
    48421 cgattatttt gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa
    48481 cagtagcagg tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag
    48541 ggatcaaaac aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt
    48601 tagctggatt agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga
    48661 tggaactgga tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg
    48721 gaacaatcat gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt
    48781 cgattttagg cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag
    48841 ccgttgtgct agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt
    48901 cagaaaaaag actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt
    48961 cattgtttcg tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc
    49021 tctttttgct cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag
    49081 ccttgattaa attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta
    49141 atgaaagtat ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc
    49201 cgttctgaat gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg
    49261 cctgccaaca tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt
    49321 tatttctata tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg
    49381 aaaaagcgtg gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt
    49441 atagagaagt tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat
    49501 ggacatgcta actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact
    49561 tttgtgattg ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc
    49621 gcagatgcaa caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca
    49681 ggctttggga ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc
    49741 aagccgatca tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt
    49801 tttggtgcga caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc
    49861 gaaacaaaag tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc
    49921 caaaaaggcg aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct
    49981 ttgaaagata cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga
    50041 ggaagagatc tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt
    50101 aatggagaca caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg
    50161 cattaagtaa taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa
    50221 ttattatcgc agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga
    50281 tttttgtcac agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg
    50341 taaaatcaaa agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga
    50401 aaaaacaagg accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaagcg
    50461 gaaggatcaa tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg
    50521 tgaatcgttc caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga
    50581 gcaaacgtgg cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa
    50641 gaatcgattg gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa
    50701 aaataagaga agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga
    50761 tgccagatga gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag
    50821 cggaagaaaa agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt
    50881 atttggaaag taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg
    50941 gtcagcaagc aatctttttg attttacaaa cgattctttc agctggagag gggatagctg
    51001 tagagtcacc ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat
    51061 ttgggatacc gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata
    51121 aaaataaatt gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga
    51181 tgagtcaaaa acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta
    51241 ttgaagacga tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac
    51301 gaacgattga tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa
    51361 caacgaaaat cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc
    51421 aagaaatgat ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta
    51481 atgaagcaat tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac
    51541 tgataagcgc ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt
    51601 actatgcctg gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag
    51661 tagatatggg tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca
    51721 gaatcaatta cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa
    51781 ttctagaaaa gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag
    51841 cttggattta aatttcaagc tgtttctttt ttttcggaaa aagaatacga tataaagaaa
    51901 ctaagaaaaa accattattt gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc
    51961 aacaaaactg tataacacca acgtttttaa atagaaacag atggttgtaa gtttgattag
    52021 aaaatagaaa aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta
    52081 atgctgttaa tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc
    52141 ttgtatttct cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt
    52201 tttattcact gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg
    52261 tgacaaatgg caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat
    52321 gcagatttcc cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta
    52381 gtacctgatt tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt
    52441 gtattagacc aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca
    52501 actgctggac aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt
    52561 cttcttccag gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa
    52621 gcttttttat ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct
    52681 ctaccaccac aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt
    52741 ttgaagaaac gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca
    52801 caaggtgatt tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt
    52861 tatatccaaa acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa
    52921 acacttgcac aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat
    52981 ccgcttgcag tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga
    53041 ccagtcctaa tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat
    53101 ccaacccgct accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc
    53161 cgcttccgta aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc
    53221 atcgaagcaa ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa
    53281 caaaaggttt cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa
    53341 caaattgcaa tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc
    53401 aagcaatcac agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg
    53461 gtgaagatgt tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt
    53521 tcggagaaga ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt
    53581 tcggtcttgc gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt
    53641 tcgaagcaat ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca
    53701 ctagaaatct acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat
    53761 tgcattcgga taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc
    53821 cttccaaccc ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg
    53881 ttgttttcct agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag
    53941 cttacgaagt tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta
    54001 tcacatacgg agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa
    54061 atatatctgt tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca
    54121 ttcaatccgt ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg
    54181 gcgttgccgc gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc
    54241 caatcggacg agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct
    54301 ggttgccaaa tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa
    54361 agaagggacg aaacaaagtc atggcttatc aatttaaatt gccggatatc ggtgaaggaa
    54421 tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg
    54481 atacattact agaagttcaa aatgacaaat cagtagaaga aatcccgtca cctgttacag
    54541 gaacggttaa aaatgtgatt gttccagaag gtaccgtagc aaatgttggg gatgtattag
    54601 tagaaatcga tgcaccagga cacgaagaca atgaagggga cagtggtgta gccgcagaat
    54661 cccaaactcc agctaaaccg gctgctgaac ccactgttga tacagaatca gctggatcgt
    54721 caagcgaagg agtcttccaa tttaagttac cggatatcgg tgaaggaatc gcagaaggtg
    54781 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    54841 aagttcaaaa tgacaaatca gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa
    54901 acgtgattgt tccagaaggc actgtagcaa atgttgggga tgtattagta gaaatcgatg
    54961 ctcctggaca caatagtgca ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa
    55021 aagtggaaac atcaggttca gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat
    55081 tggctatgcc atctgttcgt caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa
    55141 ctgcaactgg aaaaggtggc agagtaacaa aagaggacat cgaaaacttc ttggcaggtg
    55201 gtccaagcag tgcaccagcg aaatcagaag cgccagaagc ggcagcaccg aaagaagcag
    55261 ctcctgcagc agaaagcaaa ccagctgcac cagcaaaacc attcaagtcc aacttaggag
    55321 acttggaaga acgtgtagca atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca
    55381 acagtaagca tacagctcca cacgtgacat tgcatgatga agtagaagtg tctaaactat
    55441 gggataatcg gaaacgattt aaagaagttg ctgctgcaaa tggtacgaaa cttactttct
    55501 tgccatatgt cgtgaaagct ttaacagcga ctgttaagaa atacccagta ttgaatgcat
    55561 ctatcgacga tgcaaaccaa gaaatcgttt ataaacatta ctacaatatc ggtattgcaa
    55621 ctgatacgga tcacggacta tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt
    55681 ttgctattgc agacgaaatc aatgagaaag caaaattagc tcatgacggc aaactatctg
    55741 cagaagatat gcgcaatgga acaattacga tcagcaatat cggatctgta ggtggcggtt
    55801 ggtttacacc agtcatcaac tatccagaag tagctatttt aggtgtagga acgattgcac
    55861 aacaaccaat cgtaaatgct gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat
    55921 taagtttcga tcaccgtatc gtagatggag caactgcgca acaagcaatg aataatatca
    55981 aacgtctttt agctgatcca gagctattaa tgatggaagg atgataaaca aatggtagtt
    56041 ggagattttg ctgtagaatt agatacagtt gtaattggtg cgggacctgg tggctatgta
    56101 gctgccatcc gcgctgctga aatgggacaa aaagtagcaa tcattgaaag agagtatatt
    56161 ggcggtgttt gtttgaatgt cggctgtatt ccttcaaaag cactgatagc tgcaggacac
    56221 cattatcaag agtcactaga ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta
    56281 gattttgcaa aaacacaaga atggaaagaa aacaaagttg tccatacgtt gacttctggt
    56341 gttggtttcc tattaaaaaa acataaggtt gaaacaatcg aaggagaagc gttcttcgta
    56401 gatgatcaca cactacgcgt cattcatcca gattcagctc aaacttattc gttcaataac
    56461 gcaatcatcg caacaggttc ccgtccaatc gagattccag gatttaaatt cggaggacgt
    56521 gtgttagatt ctactggcgg attagcattg aaagaagtac ctaagaaatt tgtgatcatc
    56581 ggtggcggtg tgattggtgc cgaattgggc ggagcgtacg caaacttagg agcagaagta
    56641 acgattcttg aaggctcacc acaaatttta ccaacttacg aaaaagatct agtaaaatta
    56701 gtagaagatg atttcaagaa aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa
    56761 gcagtcgata acggtgacag cgtgacagtg aaatatgcag tggacgggaa agaagaatcc
    56821 gttactgctg attatgtgat ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc
    56881 ttagaacaag cgggtgtcga agtaggagaa cgaggattga tcacggtgga taaacaagga
    56941 cgtacgaacg tacctaacat ctatgccatc ggtgatatcg ttccaggggc ggcattagca
    57001 cacaaagcca gttatgaagc aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct
    57061 gtagattata aagcaatgcc ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt
    57121 atgacgatca aagaagcaaa agatgctggt atcgaagcaa aagcatataa attcccattt
    57181 tcagggaacg gtcgggcatt atcgcttggt aaaacagaag gattcatccg tctagttacg
    57241 acgatcgaag ataacgtgct tattggcgcg caaatcggtg gagtcggcgc aagtgatatg
    57301 gtctctgaat tagctttagc aatcgaatca ggaatgaatg ctgaagatat cgccttgacg
    57361 attcatcctc atccatctct tggcgaaatc gtcatggatg cttcggaatt agcattaggt
    57421 ttaccaatcc atatttgatt tgaataatac aggcgaaacg caactgaaaa atcagttgcg
    57481 ttttgttttt tagaattatc aataatatta ttatgtaaac ttaaaaatat gacaacttct
    57541 tggtttatta ccacttccta tgctacactg aagaggaata tgaaatagga gagttgaaga
    57601 tgcaagaata tcagtatcca cttgatttag attggacaac agacgaaatg gtcattgtaa
    57661 tgaagatgtg ggaagatctg gaacgtgcga atgaaaaagg gataaacaat gaagcttttt
    57721 tgaagactta tcagcagttt aaaactgttg tcaaatccat tggtgaggaa aaacgattgg
    57781 gacgggaatt tgaaaaagct tccggttatt ctttatatcg tacagtgcag caagcaaaaa
    57841 aaaatccaaa caaaactttg agaatgaacg ggtgagataa atggaacaga tgattgatca
    57901 tatcaagtcc tggcttgaag aagctggtga gaaaatcaaa tcttctcaac aaacattaac
    57961 aatctcgcaa aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa acatccagaa
    58021 ttttctaatc gaaaaaatcc attcctttga tccagaggcg aaaatattgg cagaagaaga
    58081 agggtgtaat caattatcgg atctcgatgg aagagtcttt atcatcgatc ctattgatgg
    58141 caccttgaat tttgtgattg aaggggaaaa tttttgtatt atgctcgctg tttatgaatc
    58201 cggcgttgga aagcttggtt tcatttatga cgtgatgaga aatgaacttt tttggggtgg
    58261 aaaaggaata ggcgtgtata aaaatgatga gccattgcct agaccagcaa ataaatcctt
    58321 atcggaaggc ttacttggtg tgaatagtta tcttttcggt cataataaat atggaatccg
    58381 agaaattgcg gaaagcagca tggggacacg tatgtacgga tgcgctggtc ttgaactagt
    58441 ggcaatcttg aaaggaaacc acattgctta tatttcaaac cttagccctt gggattatgc
    58501 agcgggaaat gtcttattgg aagagtttgg tatggtaaca agcggtcttt ttggtactcc
    58561 tttgacattt gaaaaaaggg aatactattt agcagggact tcttgtgcat atgcagaaat
    58621 cgcagacctt ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac aaaaatgata
    58681 aaaacgagca aaagattaat tttctcgaaa aaatgaaaac attttactat atttcatgaa
    58741 aaaagtctgc tataattagc agtcgagtaa aatttgccct gtcataaaga accaataaaa
    58801 aaggagttag ttacattgaa ttatagaaac gatatccgta acgtcgcaat tattgcccac
    58861 gttgaccacg gaaaaacaac attagttgat gaactattaa aacaatcaga tacattagac
    58921 gcacatacgc aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt
    58981 atcacgattt tagccaaaaa cacagctgta gactataaag gtattcgcgt aaacatcatg
    59041 gatactccag gacacgcgga cttcggtggc gaagtagaac ggatcatgaa aatggttgac
    59101 ggcgttgtgt tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg
    59161 aaaaaagcac tggaacaaca tatcacacca atcgttgtcg taaacaagat tgacaaacct
    59221 tctgctcgtc ctgagcatgt cgtagatgaa gttcttgaac tattcatcga attaggagca
    59281 gatgatgatc agttagactt tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc
    59341 ttgtcagatg atccagcaga tcaagaacca accatggcac ctatttttga tacaattatt
    59401 gaaaaaatcc ctgctcctgt cgataacagc gacgaaccat tacaattcca agtttcatta
    59461 cttgactaca acgattatgt aggtcgtatc ggaatcggac gtgtatttag aggaacaatc
    59521 aaagtaggag accaagtagc attaattaaa ttagacggaa cagttaagaa attccgtgta
    59581 acgaaattat ttggtttctt tggactaaaa cgtctagaga ttcaagaagc aaaagctgga
    59641 gacttgattg ctgtctcagg tatggaagat atctttgtcg gtgaaacagt tactcctgtg
    59701 gatcatcaag atgcgttacc tatccttcac attgacgaac caacattgca aatgactttc
    59761 ttagttaata attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt
    59821 gaagaacgtt tgatggcaga acttcaaaca gatgtatcct tacgagttga accaacaaac
    59881 tctccagatg catggacagt ttctggtcgt ggagaattgc atttgtctat cttgattgaa
    59941 aacatgcgcc gtgagggata cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa
    60001 atcgacggtg taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat
    60061 atgggtagtg ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga tatggtccat
    60121 acaggaaacg gacaaatccg tttgacattc ttaacacctg cccgcggttt gattggttat
    60181 tcaactgaat tcctttcaat gacacgtggt tacggaatca tgaaccatac ttttgaccaa
    60241 tatctgccaa tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc
    60301 gatacaggaa aagcaacgac ttattctatc atgagtatcg aagaacgcgg tacagtcttc
    60361 gtagaaccag gtacagaagt atatgaagga atgattatcg gggaaaacag ccgtgataat
    60421 gatttgactg tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa
    60481 gaccaaacat ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc tttggaattc
    60541 ttgaatgatg atgaatattg cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt
    60601 ttagagaaaa atgctcgtga aaaagcatct aaaaagaaaa aataatctgt aaaaggatga
    60661 agagagaaac cagcttgtta taaaaaatag ctggtttctt tttttgtcca actcttttct
    60721 gtggagaagt catctttgtc aatggacata aattcagaaa atacgccgaa agacgtttaa
    60781 aagactttta aaaaacttat ttctagagag tttttgtttc ataataagat aatgtttcac
    60841 gaaaggagat caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg
    60901 ggaagatgaa cgacttatta aaacaatact ttggctatga tgagtttaga cctggtcaaa
    60961 aagaaattat ccagaaagtc attgatcagg aaaatgtatt aggaatcatg cctaccggaa
    61021 gtgggaaatc aatctgttac cagcttccag ctttactatt agatgggctt actgtggttg
    61081 tgtccccttt aatctctttg atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac
    61141 ccgccacttt tatcaatagc tcgttagatg gttacgaaac agctagacgc ttccaagaaa
    61201 tcgatagaca gcagtaccgt ttactttata tcgctcctga acgttttatc atgccggatt
    61261 ttatccaagc aatgaagcga tggaacgtcc gtatgatcgc tattgacgag gcacattgta
    61321 tctctcagtg ggggcatgat ttccgcccaa gttatttaca gatggcgaac cagctagatc
    61381 aactgccaaa tcgtcctgta attgttgctt tgacagctac agcgactgtg caagtagctg
    61441 ctgacataaa aagactttta aaaattccag aaaacaacca tattcagact ggttttgaac
    61501 gagaaaatct acgatttcaa gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat
    61561 atttaaagat caacaaaaat caatctggta tcatttatgc agcaacgaga aaagaagtag
    61621 accgtctcta tcatttgctt aaaaaattcg atttttccgt gggtagatat catggtggtc
    61681 taaatgagaa tgagcgaaca gaaatgcaag aagccttttt atacgatcgc ttgcagctga
    61741 ttgtagcgac aaatgcattt ggtatgggta taaataaaag caatgtccgt tttgttatcc
    61801 attatcaaat tcctggctct ctagaagcat actatcagga agctggtcgg gcgggaagag
    61861 atggtctttc cagtgaagcc atcctgcttt tctctcctca agatatccaa gtccaaaagt
    61921 tttttatcca gcagtcccaa agagaagaag ggcaaaagca aaaagaatat gaaaaattaa
    61981 aagcgatgac agaatacgtg tatattgagt cctgtcttca gcaatacatc ctaaactatt
    62041 ttggcgagac aagcagtccg tgcaaccgtt gtggtaactg tttagatgac agagaaatag
    62101 tagaagtaac gacagaagca cagatggttc tttcttgctt aaaacgtatg ggtgagaact
    62161 atggtaaaca aatgctgatg aaagtcttag caggatcgaa agaacaaaag ctgcaagctt
    62221 taggttttgg acatttatct acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga
    62281 tgcagttaat tgaatatttg atatcgaatg gctatcttct gaccatcaat ggtgagtatc
    62341 ctgttttgaa agtaacagaa agagggatac aagtattgaa aggacaagaa tctgtttatc
    62401 gaaaggaacc caagaaagtc cagcagctat cggatgaaga aaccgataca ctatttgaag
    62461 tgctgcggga actgcgaacg gatctagcat cagaagcagg cgttcccccg tatgtcgtat
    62521 tttcagattc cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc
    62581 ttcagataaa aggagttggc caaagcaagc ttgataaata tggcgaagcc tttttatctc
    62641 ggataaaaaa tgcggactct aacgttctgg ataagtagaa agaacgaata cgtaaaaatg
    62701 aataaaaaca actggttaaa tctgtttttt ttgctcctta tgaaaatcaa atatgttata
    62761 attaaggaat tagaatggga ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat
    62821 ccaagtatta aatgatgaag cagaacggat caaacgatta atccgaaatc aaaacaacag
    62881 tctctgtatc tcacaatgca aagccttcga agaagtggta gatacacaga tgtacggctt
    62941 ttcccggcaa gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg agggccagct
    63001 gatgttaagt gagctagaac gagaattgaa tcagttatat agtgaggttt accaagaaat
    63061 ttatgataaa aaagaagttg gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg
    63121 catctattag actatagcat cttgattccc tatttaattt tatgtgtatt gggattgatc
    63181 atggtatata gttctacgtc gtatctatta ttagaaaacg gacaaaatcc gtccgcatca
    63241 gtcattaacc agagtatttt ttgggtgctt agcttgattg ctatcgcact actttataaa
    63301 atgaaaacag atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc agttttgacg
    63361 attttgctgc tgattgttgt gttttttggt aaggagatca atggggcgaa aggttggctg
    63421 caaattgcag gattctcgat ccaacctgcc gaatacctaa aaatcatttc tatctggtac
    63481 ctgtctctga ctttatctaa aagacaaaac agtgtacaaa aagattttct tgggactgtg
    63541 aaacgtccac ttgcaatggt tatcggactt actgcattag tcgctattct tcccgatttt
    63601 ggtaatgcgg cagttatctt tttgattatt ttggttctgc ttttagccag cggagtcaat
    63661 tatgtgtata ctttgattgt aggagtagga ggcttttgtc taagtacttt tacgatctgg
    63721 ctgatcaata tcacaaacgg aaagatctta cccggacgtt tacagtatat ctataatcga
    63781 tttgctattt accaaaatcc tttctcagac gaattaaata aagggcatca actagtcaac
    63841 ggctactatg ccatgttcaa cggaggacta tttggcaggg gattaggtaa cagtatccag
    63901 aaaaaaggat ttttacaaga agcacaaacc gattttattt atgcaatcgt agtggaagaa
    63961 ctgggagtga ttatggggat tttgatatta gcgttgctat tctttatgat tgtgcgcatt
    64021 gtccttgtag gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc
    64081 gcaatgtttt tgatccaagt atttgtcaat cttggaggaa ttactggcgt tattcctttg
    64141 acagggatca cgttcccatt tttaagtcaa ggaggctcca gtttgctgat gctttcgatt
    64201 tgtgtcggct ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt aggtttataa
    64261 taaaaaaaga tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat
    64321 cgctgtacgt gtatttcgtg cttgtacaga attaggaatt aaaactgttg gaatctatgc
    64381 agaagaagat gaatattccg tacaccgttt caaagcggat gaagcatatt tagtaggaca
    64441 aggaaaaaaa ccgattgatg cgtatctaga tattgaaggg atcatttcta tcgcgaaaga
    64501 atgcggagct gacgctattc atccgggtta cggtcttttg tcggaaaact tgaattttgc
    64561 gcaacggtgc gaagaagaag gcatcatttt tgttggaccc aaactgcatc atttagatat
    64621 ttttggagat aaaatcaaag caaaagctgc agctatcgaa gcggggattg cttcgattcc
    64681 aggcacagat ggaccaatcg cttctataga cgatgccttg gagtttgcga aacaatacgg
    64741 ttatccgatt atgatcaagg cagctctagg cggcggcgga cgcggcatgc gtgtagctca
    64801 cgatgaaaag agtgcaagag agggctacga acgtgcgaaa agcgaagcaa aagcagcctt
    64861 tggtagtgat gaagtgtatg ttgaaaaata catcgctaat cctaagcata tcgaagtaca
    64921 gattctcggg gatacgcatg gaaacgtcat ccatcttttt gaaagagatt gttctgttca
    64981 gcgccgtcac caaaaagtag tagaagtagc gccttgtgtc tctatgaatg aacagcaacg
    65041 tcaaaaaatc tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg
    65101 tacagtagaa tttcttgttg aaggagatga tttttacttt atcgaagtca accctcgcgt
    65161 ccaagtggaa catacgatca cagaaatgat tacagatatt gatatcgtaa caacgcaact
    65221 gttgattgct caaggtttgg atctgcataa agaaatcgga ttgcctcagc aagaaggaat
    65281 taaattaaat ggttcagcca ttcaatgccg aatcactaca gaagacccgt tgaataactt
    65341 tttaccagac acagggaaaa tcgatacgta tcgttcacct ggtggttttg gcgtacgtct
    65401 ggatgtcgga aatgcttacg cgggctatgt agtgacaccg tattttgatt cattgctagt
    65461 aaaagtatgt acgcatggtg ctacctttga aacagccatt cagaaaatgg aacgttgctt
    65521 gaaagaattt agaatccgtg gagtgaaaac aaacattcca tttatgctca atgtcatcac
    65581 acatcctgaa ttccaatcag gaaatgcaaa aacaaccttc attgatagta cagcaacatt
    65641 atttgaattt cctcgactac gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat
    65701 tactgtgaat ggttttccag gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg
    65761 tatgcctaaa gatctaatca caagaactga ttacgtaacg gctaagaatg tattagatgc
    65821 gaagggtgcc gatgctttgg tcgaatggat caaaggacag gaaaatttgt tgttgacgga
    65881 tacgacattt agagacgccc atcaaagtct gctagcaact cgtgttcgga caaaggattt
    65941 caagcagatt gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg
    66001 ggggggagca acatttgatg tggcttaccg ctttttgaat gaagatcctt ggcaacggtt
    66061 gagaaaaatt cgttctttaa tgcctaatac gttgctgcaa atgcttttca gaggttcaaa
    66121 tgccgttggg tattctaatt atcctgacaa tgtattggtg gaatttgtca aagaagcagc
    66181 agcgcaagga atcgatgtat ttcgaatctt tgatagtttg aactggactc cacaaatgga
    66241 aaaaagtatc caagctgtac gggatactgg taaaatcgca gaggcagcta tttgctatac
    66301 tggcgatatc aacgatccaa gtcgggcaaa atacaatgtt cagtattata aagacatggc
    66361 aaaggaacta gaacaactgg gtgcgcatat cattgcaatc aaagatatgg ctgggctgct
    66421 taaaccgcag gcagcttatc gtttgattag tgaattgaaa gaaacgacag atctgccaat
    66481 ccatctgcat actcatgata ccagcgggaa tggaatcatc acttattctg ctgcttcaaa
    66541 agcaggagta gacatcgttg acgtggcaat gagtgcaatg agtgggaata ctagtcagcc
    66601 tagcatgagc agcttgtatt atgcactggt caatggtcca cggctgccag aaatcacgat
    66661 tgaaaatgca cagaaattaa accattattg ggaagatgtg cgcatgtatt acaaaccgtt
    66721 tgaaaacggc ttgaatgctc ctgaaacaga agtatatatg catgaaatgc caggaggaca
    66781 gtattcgaat ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat
    66841 caaacaaatg tatcatactg tcaacttgat gtttggcgat atcgttaaag taactccatc
    66901 atctaaagta gttggagata tggcactatt tatggtccaa aatgatctta cagaagaaga
    66961 tatttatgaa aaaggcgaaa cactaagttt ccctgaatct gttgtgactt tcttccaagg
    67021 agaactaggg caacccgttg gcggatttcc taaaaaattg caaaagatta ttcttaaagg
    67081 cagacctgca ttgaatgaac gtccaggtct tttggctgaa tcagtcgact tcaacgaagt
    67141 aaaaaaagaa ttggcagaaa aaattggcta tgaaccaaaa caagacgaag tattaagcta
    67201 tttgatgtat ccgcaagtct ttttggacta tcaaaaagca tataatcaat ttggcgatgt
    67261 tacattactg gacacaccaa catttttcca aggtatacgt ttgggtgaaa caatcaatgt
    67321 acaaatcgaa cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag aacccgatat
    67381 cgaagggaat cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa tcactatcaa
    67441 agataattcg atcatcagta cggtccaaac ccgattgaaa gctgaaccga caaaccgaga
    67501 acaaattgga gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa aaggcgataa
    67561 cgttaaaaaa ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa cggccatcga
    67621 agcacgtttt gacggagaag tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc
    67681 tggtgacctt ttgattgaag tgactgagaa ataataagcg aaaggtgggg aaacatgaaa
    67741 agactgggtt tgttttggct gattctgcta ttagttattg tggcattcta catggaacca
    67801 gtcttgtttc cgccaaaact tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa
    67861 atgacaacgt ggaaatacca ggagcttaaa gcaactggtt tttctactta tatcggaaaa
    67921 ccgatcgatc agctggaaaa ggattttggc caagcctatg atcgattaac tagcggattt
    67981 ggttttgaaa caagatatta tgaaaataaa gctagtcatc tcacatttga agcgaacatc
    68041 caagacagca gagtcacaac tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg
    68101 ttttatttag ggatgacgat gcaagatctt acgaaaatca ctactatcta tactaatttc
    68161 acttttgagt accatgatac agaagttgga atcgaactaa tggaagagga tatgaattat
    68221 cgtccgctaa ttgcttttga taacgacact tttgctattt tattttttga tcaaacaaca
    68281 aacggcctat tttcagttgt gtatttagat aaagatagtc ttttgaaact attgccatat
    68341 caagtgtttg gtgaaggcct tccacattat caagcagata aatcggctga ttgggaatca
    68401 atcaatcgcg ttaaagaacg aaaaagtacg actttattaa atatgcttag aagagaagac
    68461 gaacttccca tatacaatca aacacttgcc tttcaaaacc acagtcagct gctactacat
    68521 gattttttga caaggcctga ggaaatattg tcagaggaac gtatggctga ttggcaaaaa
    68581 tcgctaggca gtgctcaaac gacagtgaaa ttctcactga cgaatgatga attggagaag
    68641 ttaatcagta aacaaaaatt agaaaatact agtgggctat ttattcatcc ggcgattgac
    68701 gctacttttt cgtttttata ttggtacagc gatccttaca accatgaccg tttcatggac
    68761 ccgggaacgg attctgtagg cattgctttt tctaaagaaa atatgttagt cttattgcaa
    68821 gaagaaaacg gagaaagtag tgatagcagt gatcgttaac gaagagctgt ttcaactgga
    68881 ggatcaatgc ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct
    68941 tgaacataaa cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag aagcgttctt
    69001 gtctaagaaa gaagcatttg aaaatgtagc ggcatatggt gagtatgcac ctgattatcg
    69061 tgaaaaaaaa cgagctgttc gaaaagcaaa aagacagctt gatatggatg agcatgttgc
    69121 agctttccga tatagtgaaa cacagctgca aagtctgttg gatcaaattg tccaagtact
    69181 tgccgaagaa gtttctaatg aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa
    69241 aaaacacttt ggatgcggag gtaattgtca tgcaattaga caatagtgaa tttgaaataa
    69301 caaaacgacg cgggctgatc gtatgggtct acagcttaaa acaattaaag aatctgaaaa
    69361 agttcggctt gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg
    69421 aagagacatt ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag
    69481 aacgttctta ccgaccggat atcgaaatga attttgccga aaaaatagga aacaaaacag
    69541 tattaaaaga cgaaggtttc gaaattgaag agctaaatac agaaatccga ttagctgaac
    69601 atctttaaaa atctcttatg agcagttttt aaataaaaaa taacttggat aacgatcgat
    69661 ttttgatcat tgtccaagta tttttttggt atactagtac cagtaccatt tctaaggagt
    69721 gagtagatgc ggataatatc aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg
    69781 gcgaacacca gaccaactac agataaagta aaagaatcca tattcaatat gattggacca
    69841 tattttgatg gagagacagt attggatctg tttgcaggaa gcggtggttt agctatcgaa
    69901 gcagtatcta gaggctgcag tcatgcggta tgcgtagaca aaaattacca agcgctaaaa
    69961 atcatcaaag aaaacatcga aattacgaaa gaaccagaga aatttaccac aataaaactt
    70021 gatgcggaca aagcaatcga acggctagca gcggagaaac aacaattcga ctacttgttt
    70081 cttgatccgc catatgcaaa acaaaaaata atcgatcaaa tccataaaat ggaagaactg
    70141 ggactttttt ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt cgaattgcca
    70201 gaaactgtgg ctggattcca tcaaataaga aaacagattt atggcataac ggccgtaact
    70261 atctatagaa aagagggaaa tcaatgaaac gtgcattatt cccaggaagt tttgatcctt
    70321 ttacaaaagg tcacttagat acggtggaac gtgcggctaa attattcgat gaggtagtaa
    70381 ttggtgtttt tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat
    70441 tgatcacaaa agctgtttct catcttccta atgtaaaagt catgcatcaa gaaaatcaat
    70501 tgacggtaga aacagcaaaa gagattggtg cagatgcgtt gatcagaggc atacgaagta
    70561 tcaaagactt tgaatatgaa cgagagattg ctcagatgaa tcatcatcta tatgatgaat
    70621 tggaaactgt cttcttatta gcaaaaccag aatatagtca cgtgagttcc agtattttaa
    70681 aagaagtact gcactttggc ggagatgtga gcagctactt accgccagtg atcaacgaag
    70741 ctttagctgg aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt
    70801 atcatatttg gaatgttgat cattggattt gtagtagttc ccttgccgta ttatgtagaa
    70861 tcaccgggag ccacgatcga tctgaaagaa ctaattacgg tcaatgataa ggaagataaa
    70921 ggttctgggt ctttctctct tacctctgtt ggtatcaggc aagctacagg tttcttagct
    70981 ttaaaagcta aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt gactggtggg
    71041 gcgacaaatg aagaatataa tcaaatgcaa cagtattata tggattcttc ccaaaatgca
    71101 gcaatcgaac aagcgttaaa acttgcagac aagccttata aaatggaatt caaaggcgta
    71161 tatgtcatga gtgtagagaa aacatcgaat ttctttggca agctttcagt tggagataca
    71221 gtaacaaaag tagacgggaa aagctttcaa agtacagaag catttatgga ttatgtaaaa
    71281 agccaaaaag ttggacaaac aatcgaaatc agttatttgc ataatggtga agaaaaaaca
    71341 gcttctggtg atctgatcga gttaccaaca gataaaaagg caggaatagg tatcacgctt
    71401 actgaccata ccgagatcga atcagataca gatgttcgta ttaattctgg ctctatcggt
    71461 gggccatcag cgggattgat gttcacttta gaaatctatg aacaagtcac tggtaaaaac
    71521 ttgcgacatg gtaagcaaat tgcaggaact ggaacgatca acagtgaagg agaagttggc
    71581 cgaatcggag gaatcgataa aaaagtggca agtgcggata aagctggcgt agagattttc
    71641 tttgcaccag atgatacgat ttcagacgat gttaagaaag aatatcctga aataaaaacc
    71701 aactacgaag aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca
    71761 gtaaaaacag tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt
    71821 aaagaggggt tgtgacacga gttctgtcac aacccctctt tttcgtcaaa aatcttcacg
    71881 ataaaaataa aaacaaaaga aaaaattgac gtaattagat gttgaaggga cgattttgcc
    71941 agagctcact gtttcttcaa ttttaaatga aaggagtaat gaatgatttt tactggtcat
    72001 tctttgaaaa aagaacaatg atagaaaaaa tcagcaagaa aatactagct tcacccaaat
    72061 tgatcatttg gatgtctatg atagttctta tcttttgtat cgcaagtggt tttttattat
    72121 caaatttgct attttataga gaaacagaag agactgctgc aaaagaaata ctttcttcgt
    72181 ttagttccag tggagaagat ccatcagctg aaggaaacga tgtagaagag gatgctcgtc
    72241 caaaggtcat gtacaccgat atcaaaggaa gtgtgaagga gcctggtatc tattcctttt
    72301 cttcagaaga acgggtctat gatgtactaa agcgtgcagg tggattatta gaagaagcgg
    72361 atagcgatcg aatcaacttt tctgcgaaga tagaagatca gcaagtccta tatatccctg
    72421 ctgtaggtga agagctgcca gaacatcttg atcagttggc atcctcggaa ggaaaacagc
    72481 ctacagcaga ttccgagcct cccaaaataa atatcaatac agccactcca tcagaacttc
    72541 agcagattcc tgggatcgga tcagtcaaag cgcaagaaat cattcgattt agagaagaaa
    72601 acggatcctt ccagaaagtg gaagacctgc aggagatttc tggtatcggt gaaaagacag
    72661 ttgaaaaact aaaaaacttc gttacaatta aatagaatga ttcaaaatcg actggcacga
    72721 gcaataaatg gacgctaatg gatactaaga ataatccaat tctaagagct tggcaggacg
    72781 aggaggtagt atttttggat aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa
    72841 ccaatgctga atgtctatct ccaaaataaa atatctggga ttcatattat gaatacggct
    72901 gtcagcggca ataacagtca agctctacga gaacgctttg ccaaagatgt acttagctat
    72961 acagcagata aagtatttat tctcattggg acgaatgatc tagcagagca taaacaactt
    73021 tctaaagaaa cgtaccaaaa aatctgcagt ggatgatagt tcagctgaaa aaagactata
    73081 gttctgatca tgctttcttt atcacgccac cagcagtcga tgagacaaaa caaaaatatc
    73141 ggaataacaa gttgatcaaa gaatatatct ctgttctcac agaagtagca aaacgccatg
    73201 attgtgaagt atcgattttt atacggaatt tcttgaacaa atggattcga agaattttta
    73261 taatgggcta atggatgacg gacttcattt cggcagtgaa gggtaggatc ttttgtcaga
    73321 caggatgatt cgttatattt aaagaatatt ttgtttaagc aatgagtact ttttaatcaa
    73381 cttgaaaaaa cggaggaata taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga
    73441 ataaagaaaa agttactgat aagtcacata atgaacgcat tccttgggac cagtacttta
    73501 tggctcaggc agtactgtta tctttacgta gtacgtgtac acgtttagaa gtaggagcga
    73561 cactcgttcg agaaaaaagg atcattgctg gcggctataa tggagcagtc agtggagatg
    73621 tccactgcat agatgaaggg tgttacgtcg tggatggaca ctgcttgcga acgatccatg
    73681 cagagatgaa tgcactccta cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa
    73741 tctatgtcac acattttcca tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga
    73801 aagggatcaa ttatctccat gactatcgaa atgatcctta tgcccaagcg ttgatcgaac
    73861 aattaggtgt atctgtacac caagttgctt tagactcaca gcatttttca aaacttcaaa
    73921 atgaattaac aggaaaacat gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt
    73981 tttccggcaa ttacgtcgat gattatctgt ttgttgtatt acactgacaa ttggggttat
    74041 cgtttgcttc ttgccggaat ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg
    74101 tacttgtgta ttttgtgtag cataacggta tccatttctt ataggctaca agagtattca
    74161 tttttagagg aaaaaacaac atctgcgcaa attactgtat ttacagatac gattcgtgca
    74221 aatggtaatt ttttgacaat ggaaggaagg ttatcagaga ataaacaaaa gctccagctt
    74281 tcttatcaag taaacacaga agcagaattg aaaacatggc ttaacttccg aggaaaggga
    74341 atagaattga ctgctgaagg cgagttcatc tctccagcaa aaaagcggaa tctttataat
    74401 tttgaccaag aagcttactt caggaaccat agaatttctg gacggttcca aattcaaaag
    74461 attgaacaga tcaccatgca aaatcactgg cgaaactggc ttcagagaaa aagaggatct
    74521 tttatctcct ttgtccaatc tgaatttccg caaaaaacaa gtgtatatat taattcttta
    74581 ttatggggct ataaagatgc aggattcaga gaaccagaag aaatttttag ggagagtggt
    74641 ttgctgcatc tgttcagtct atcgggtcta cacatccaat tttatttagg gtggctgtat
    74701 tatttatttc gacgtatcgg atggccacta cacgtcagta ttattccgtt gagtctacta
    74761 actatctgtt atgtttctat tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg
    74821 tttttattgc gattgctagt caaattgctg aataagcatt attccaccat ggacttattt
    74881 gcagttgtct tttggcttct tttactattt gatcctcgat ttctttttca tgcgggtgga
    74941 cagcttagtt ttttcatgag ttttttgttt atcctgattt cttttgatgg agattggttg
    75001 aaaaaaacgg cctctcacgt cctgtttact atgatcacga tgccgttgat tgtatggcat
    75061 ttttatgagt ggcctatttt aggtagtctt tttacattac tgattgcacc ggtattcaag
    75121 tttctttttc ttcctggtgt gttatttctc acactcttca atcgctggct gccggaaact
    75181 ttacttttgt tgatagaaga gggattaatc ttatttgagc gaataatcga tttttcttca
    75241 gggtcgtctc tcattatagg acgtttgccc cttgtgctat ctgggatgta tattttcgga
    75301 ttactgattt gtatggatag gttgaaagaa caacctgtga aacacttgtt ttatgttggt
    75361 cttttttctt ttattttttt gactttgcca tttttacgct tttcttcaac gatcgctttt
    75421 atcgatgtcg gtcaaggaga cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt
    75481 ttgatcgata ctggaggtca gttacacttt gaaagagaaa agtgggcgca aggtatgata
    75541 cgtcctcctg ctgaaagaaa tatcgttcca tatctaaaag gtcaggggat aagtgtaatc
    75601 gataaactca tcttgacaca cgacgatacg gatcatgtgg gagaattacc cacgttatct
    75661 cagcatttta ctatcaaaaa aatttatatc ggttggggcg ctggacgaaa tgagccatta
    75721 caaaggcatt tatctgaagc tcaaaaaaat ggtactgaga tcattgaaat caaacatggg
    75781 gacaggatca gcggttatta tgatctttat gtattgactc cttttgaaaa gggagaggga
    75841 agaaatgaag attcgattgg tctatggatg gaatataaca ataggcgctt tttattttta
    75901 ggagatttga atcaagcaat ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc
    75961 gatgtagtca aattaggtca tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc
    76021 catattgaag caaaacatgg gattatatcc tgcggcgtaa ataatcgtta tggtcacccg
    76081 cacagcgaag tattggagac actagaagaa aatcagataa aaacatggcg aacggatcaa
    76141 caaggaatga tcacctatcg ctggcatcca gtttttcatc ctcatgggct agtggaaaca
    76201 atgatagact agtaattctc atctgctcat ggtaggataa tttttgaaag tggtgagtaa
    76261 tttgagtata caagcagagt tacaaaaaat acgtaaagaa aaacttgcat cttgttactt
    76321 gattttagga acagaaaagt ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat
    76381 ccaattagag ggcgaagatg acttaaattt tttaagtttt gacatggaaa atgcaagttt
    76441 agatgaggta gtagctgaag cagagacgtt gccgtttttc ggagatcaac ggttagtatt
    76501 cgtagagaat ccttattttt taacaggtga aaaagtaaac aatggaatcg aacaaaatac
    76561 tgatttattg acggcttatt tgaaagaacc attagaatcg actgtattag tattctttgc
    76621 gccatatgaa aagttggatg aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat
    76681 tatcatcaat gtccaacagt tgaatgaaaa agaagtacgt caatatctga tgaatacctt
    76741 agagaattcc tcaatcaaaa tggaacgaag tgcaattgat ttattcttga ggttgaccga
    76801 cttagattta tccaaaatga tgagagagtt acagaaattg gtcctttatg gacaaaatca
    76861 acagcaaatc acggtaagag aagtagaaga attagttcct aaaacgttag aacataatat
    76921 ttttgacatg acccaatata ttttaaaagg gaagacggaa caagctttgc gtttatacga
    76981 agatttagtt atgcaagggg aagaaacaat caaaattaat gcgattttgc tttctcaatt
    77041 acgtttattt ttacagacaa aatttcttgt aaagattggt tatcagcagg caaatattgc
    77101 tgaaacattg aaaatccatc catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt
    77161 cgatgaacat ttgctggtac agttgtttga tcaattagtt gagatggatt atcaaatcaa
    77221 aacgggacaa atcgaaaaag aattaagttt tcaactattc gttttgcgca cagggcagtt
    77281 gttaagaaat cgataagaag aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt
    77341 gtcctttttt tgacaaataa aaaaatcggc cgaagccgat tcttttattt agcgattttt
    77401 ttgcttaaac gagatttgtc gcggttagct ttgtttttgt ggattagacc ttttgattca
    77461 gccatatcaa tagctttaac agcttcttta tataacgcat ctacgttgtc agcacctgaa
    77521 gctacagcat cttcaaattt tttgattgca gtacgcatag cgcttgtttg agatgaattt
    77581 ttcacattag cgttttcgct tgtacgaaca cgtttgattg cagattcaat gtttggcatt
    77641 agtttcacct ccgataactt aagttcttac gcgtgttacc gcataattca acattcatta
    77701 ttatacctaa aggcagcgtg ctttgcaata tgaacatgac aagttttttt ccttaacagt
    77761 cttttattag gatttttttt cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag
    77821 acgctattgt gggaggcaat agcgtcaaac caaactagat ttttatttat ctatttaaac
    77881 aaagtttgaa tgggaatgtt tggtactaag ttattatata acaaagagaa ccgtttgcaa
    77941 agtttttttt gaaaagggat acgattccct gttgttcttt gtttttctat taagaaactg
    78001 cagttatttt ataaccagta tcctttgatc agtaaaggct attctggtgt accgattatt
    78061 ttaatgtatt catcgatatt ttttatcaac atttttttta tgaccaactg gttccgacgg
    78121 ttccatttgc cacttacttc cagcacatat ttatttaggg gcaaacgtaa gatatatttt
    78181 gccaaatagc gatctttaag caggccattg atcacacggt cctcaagctg caagttgatc
    78241 ccagtcagca tcattggctg ttcttgtaaa acgtacgtat caattaaaag accttcaaaa
    78301 gtttccacaa gcgtcttcct ttcttttcaa agtaacctga aaacaggcac taattcttcc
    78361 attttgatat tcgttgaagt ggatgcgatc attctttctt ttgtccactg taatctagta
    78421 taacaaaaaa gagcagaaga gccaaaaaga aaaaagagca tcgaattcta taggaagtct
    78481 cattggcacg gcctaaatta ttacaaaaca ttgattaaaa aaagcccctg catcgcagga
    78541 gcatccccta tatcgtttta ataggtcaat caaaaagcag tatcaaacac attttgtatc
    78601 aaatcaggaa ataatagata agctaagccc agaatcaaaa gtaccgctag aagaaaccag
    78661 ccgattgttc ctatgaaata ctttaatttt tcgaacattg tatcacctcg ttgtcagtat
    78721 atcacggatc agtaaattgg gggtgttaag aatctcctaa aatagacaaa atttgttcta
    78781 ttttcctaaa gcaaatactt gtaagataca gtaagtgata ccagaagcaa tgaccggtcc
    78841 ggcggcgatt cctcttaaga agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt
    78901 gatttgtgga tcagaagcaa tcaatcctac acccttggaa gataagacag ccactagccc
    78961 accgcaaaaa acagctaccc agccaatagg ggatttaaag gcttctaata gatcacgtag
    79021 accgatttct cctgttgcga tagggattag aatagccgct gtgatgatcg tcaccccaat
    79081 attgattcct ttgccttgaa gccaagggaa aaaggaacgg gaggctggta tgagcttcat
    79141 aatcaaaaca aaagctgccg ctatcatcag actttgattt ttagctaaaa ttgctacaag
    79201 taaaataatt cctaaaaaaa gccaactttc catgaaatat attcctcact ttcttcgaaa
    79261 caatcataac atgtttttag aaagttacaa aagtaggaat tcatccttca agacctattc
    79321 ataagctgtt tttttcataa aataaacaaa atcataaaaa aacactttgc ttatttgaca
    79381 gaagacagcc gcgctttgta tagtggacaa gacttacatt tttttgaaga atcagaaaga
    79441 tcaggtaaag gtcttagtaa acgcaagcca ttcagaataa ccaatattgt gcttccttcg
    79501 tgtcctacaa ctccaagagg tagatttaaa aattgcagga agttagaagc aatcaacaag
    79561 agaataacaa gaatcgaaaa aataatattt tgtttgatga tccgccgcat ttttaaagat
    79621 agccgatgac tgtagacaaa tttatttaag tcgcttttca ttaagacgat gtctgcgata
    79681 tccatagcgg catctgtccc ttgccccata gcgacaccta aagaagcggc tgctaaagca
    79741 ggcgcatcat tgactccatc accaaccatc gcatttactt ggtaaagttc attctctttt
    79801 ttgactagtt gtgttttttc ttccggagta cagcttgcat agtaatgttc aatccctact
    79861 tgttcagcaa tggcctttgc tgtgccagaa tggtcgccgg ttatcatact tgtatggatg
    79921 ttttgggatt taaagtagga aattacttgt tgggtatttg cttttggaat atctagcaga
    79981 cctaaaacag caattaactg ctgatcttta gaaagataaa tcacggtttt gccttgattt
    80041 tccaaacgtt cgatcatttc cgcaatctct ttgctgatta tcatcgtctc aggatcatat
    80101 gcgtgcttgc caactttcca ttgcgcacct aaataggtag tttgtaatcc aaaaccagta
    80161 atttcttcta ttggtaagta ttggatttct tcaggtattg tgcaatcgaa atattgcaaa
    80221 atcgcatgag caagtggatg cgtcgttttt tgttccatag caacaagaag ttgttgtgcc
    80281 aaatcctttt catcaaagaa aaagctgtca gttacaactg gttttccttt agtgagcgtt
    80341 cctgttttat caaaggcaat cgcttttaat tcagaaagct tttctaaatg gataccgcct
    80401 ttgattaaaa tcccattttt tgcagaattt gaaagagcag ctagagaagc tggtgtagca
    80461 gatgcgacaa gcgcacaagg agaagccaca actaataata ccattcctcg ataaaagctt
    80521 tcatcccaac tccaacctag gaaaaagtgg gggagaagga tcattagagg aacagcaagc
    80581 aaaatcaatt ttacataatt attttcgatt cgttcgataa atgaagctgt ttgagttggc
    80641 gtattctgcg cttcttctac taattggata atcttagcaa agacagtttc ggaactcgtt
    80701 tttgttactc gaatcgtgat gggatttcct tgattcagcg ttcctgcata catctcatcg
    80761 cctaaatatt tttctacagg cacagattca ccgcttaatg cagactcatc gatcgttgcc
    80821 tgtgtgcttt ctaaaaagcc atctatcgga acagctgctc ctttagcgac aaataccaag
    80881 ttatccacct ttagttgaga aacatctact tcttgcattt gtccattttc ttttaataat
    80941 tgggcttttt gtggttgaag attcatcaaa gcagtaattt ctttttgact tttattagtt
    81001 gtgtattcct ctaatgcacc gcttaaacaa aagatgaatg tcaacattgc tccttcaaag
    81061 taattaccga tcaaacaggc accaatagca gctaaagcca tcaataaatc cacattcagt
    81121 gtccgatcat tccacaattc tttcaagcct tccgatgttt gtttatatcc accgatgata
    81181 atggcactga tataaaaaag tggggtgatg gtaagagaga atcggtcgaa gataaaaccg
    81241 aagatcataa ataaaaggca gccgattgtt gctgcaaaag cttttttctg attagtcatc
    81301 gttattagac ctccttgtca aaacaaggat agcataataa aaatgtaatt gataataata
    81361 acagctaaat gagaacgata atggttatca tttggtgatt aagcttgatg ttgtttttat
    81421 tatttataat gattattatt aaagaaaata aaaaaccaga acatttctca aataagagaa
    81481 gtgttctggt tttttctaca ttgcaataaa ccaataacca atcaaaatga taccaaggat
    81541 aatacgatac cagccaaaag cggtaaagtc gtttttcttc aaatagctta gtaagaattt
    81601 gatagctaag acggaaacca caaaagatac gaatgttcct acaagaagaa caacagtttc
    81661 ttccattttg aatgaattcc cttcagctaa atatttgacg atcttcaagc cgcttgcgcc
    81721 aaacatgaca ggaatcccta agaaaaatga aaactctgta gcgacaaaac gcgaagcacc
    81781 aatcaagata gatcctagga tcgttgcgcc agaacgggat gttccgggaa caagtgccaa
    81841 gacttggaat aacccaacaa gaagagctgc ttggtacgta aagtcattca gagaagtcca
    81901 acgaggtgtt ttgtttttgt tgcgtttttc gacaataata aaggcaatac cgtaaacgat
    81961 caaaacaatg gcaactggta agaacttgtg gaaatttgct tctaaccaat catccaaagg
    82021 caaaccaatg atgatggctg gtatgcaagc aacgatcact ttaaaccaaa gtgtccacgt
    82081 atcttttttt tctaccgttg atttacttgg agaaaatggg tttaacttgt gaaaataaag
    82141 aacgacgaca gccaagatcg cccctaattg gataacgaca ttgaacatcg acataaattg
    82201 tgaactggca tccagtttga tgaattcatc tgctaggatc aaatgaccag tactactgat
    82261 gggcagccat tccgttattc cttcaataat accgagaatc actgctttga taatatttgc
    82321 aaaaaacata aattccttcc ttcccaatta agatacatct tatagtatac cgttattttt
    82381 tgagaaaccc aatctgtttt ttaatattaa gaaaaacttg agaaataaag cagataagta
    82441 cacgaagaga aaaaagaaga caaagccctc gttcagactt tgccttttat gattctctgg
    82501 taaaaattaa aatatcccca ttatagaaac gagtcgcgac tatgtgtttt cccgttatcg
    82561 atataagacg tatcattgat gccgtggata gaaaatcgac cgtttgcata agtgaccttt
    82621 gttacgctgc catttaaaag cccagcacga acaggctgtt taggatcgat cagattaagc
    82681 aaaaaggcga tcgtcaaacc atgagatacg actaaaacat ttccaccacc atttttttcg
    82741 cagtgatggg cgatatcttc aaatcctgtc catacacggt ctcgcaatgt ttcataagtt
    82801 tccgcccacc cagcggtatc taagttgtat acagcattag caatttgttc aaaagaaact
    82861 tctgtggtaa acatttcatc atatgtttta aaattcaata cacgagggat cacaccccac
    82921 atttccatgt catatccacc ttcaagagac ccgaaacacc attctctaat ccttttttca
    82981 ataaaataag gaatttcttt tcccttagaa tgttctccta gaataatacg ggcagtttcg
    83041 attgcacgac cactgtcact agaataagca aatgaaaagt cgacattttt taacccgcga
    83101 cctaaataat gaatgccttc ttgtccttgc tttgtgaggg gtgtatcaca ccagccttgt
    83161 actcgttcaa ttgtattgaa catcgttttt ccgtgtcgta cgatataaag tgtagtttct
    83221 gtcattttat tctcctccta agtcgtttta acgattgaaa aaaggatttg tccttttttc
    83281 ctgttcaatc gtcgttgcat cgccatgtcc tggatagact gcaaattcat caggtaatac
    83341 aaacagctgg gtccgaatac tatgaagtaa ttgttctaaa ttccctgtat acagatcggt
    83401 tctaccgata cttcctctaa acagggcgtc gccagaaacg acaaaatcat caaaaataaa
    83461 gctgacgctt cctatagaat gccctggggt agggacgacg cgaaaagaca tgtttcctaa
    83521 agtataagtt ttcatttcaa attcatattc tgcaggcgag acaataacat cagcaatatc
    83581 atcatgtcta ccgagtccag aaagattcaa aatcggatca cctagccatt tttgctctag
    83641 tgggctgaca taaactggga tttgataata cgcgcgtaaa ggttccactg caccgatatg
    83701 atcgtagtga gtatgggtaa gcaaaatcgc taccggctgt tggtttgttt ttttgatttc
    83761 ttgtttgatt cgttccgcat cttctccagg gtcaatgatc aaaagtgctt cttcgttgta
    83821 gaccaaataa cagttttctt caatcattcc tgtttttaat ctttcgatat gcatgtgcat
    83881 tcctccttga tagccaaaac ttctttcatc cattagtata acgcattcat tgctaggaaa
    83941 caaaaacagg tacttatttc tctttactta tctgaattat cagactatat gaaaagtatt
    84001 tggatattct aaagagaagg aaagtaagtt ttcatagcat tttaatatgt ccttgttaca
    84061 ttagattcat ccaaaaatcc aaattctata tcctatatcc ctgcctaatc agcagggtgt
    84121 ttttttatgt ttggaaagag agctcttttt gctaaactag gttttttcag gtaatcttag
    84181 agagaaagag gtggtggaaa tgatcaaaca gtcaaaaata tctgtgcggc acttagtaga
    84241 atttattttg cgacgtggaa gtatcgacaa tcgtaaaaaa agtaaccata ctgcattaga
    84301 aggagcgaga atccaccgca agctccaaaa agatgcggga gaaaactatg aaaaagaagt
    84361 ctttttaaaa acaacagttc aattagatga atattacctg atcgttgaag gccgtgcaga
    84421 cggtattttc aaaagagacg gtatttatta tatagatgaa atcaaaacct ccgaaccacg
    84481 atttgaagat ttagaaccag aacaagtgga acttttcttt catcaagcaa gggtgtatgc
    84541 ctatatctat agtcaagaac atgatttaca agaaataaat ctgcaattga cttacttcca
    84601 gacggcagaa gaagtgatca cgagaaaggt tgaacatcag acaagagagc agttagaaac
    84661 tttttttaaa aagctgactg aagattataa agaatggctt gtttttcaag aaaactggcg
    84721 cactgtacgc aatgcctctt tgatggcttt gaaattcccc catgatgagt ataggaaagg
    84781 tcagcgtgaa ctagcggtag ctgcttataa aacgatccgt acgaaacaga aattgtttgt
    84841 agaagcacct accggaacag gaaaaacaat ctctacgtta ttccccgctt tgaaagctgt
    84901 aggggaagaa gagggagaaa agatctttta tctaacggct aaaacaataa ctagacaagt
    84961 agctgaagat gctatgacag cattaaaaga tacaggagct gaagtaaaaa gtgtcacttt
    85021 gacggcgaaa gacaagatct gctttttaga tgagacgacc tgtaatccag atcagtgccc
    85081 atatgccaat ggttattaca atcggattaa tgaaggttta tgggatcttt tgaatcatga
    85141 gaatcagatc actcgtgaag tcatcgaaac ttatgcaaga aagcatacac tttgtccttt
    85201 cgaactatca ttagatgtca gtatctggtg tgacgtaatc attggggatt acaattattt
    85261 atttgatcca actgtttatt taagacgttt ttttgaagat gaaaaaaacg aggactatct
    85321 gtttttgatt gacgaagcac ataatttggt gaatcgttcc cgagagatgt attcggcaga
    85381 actatcttat gaaaaaacga aacgaagtaa ggaagctata ccaaaggaat tcaaaaaact
    85441 tcatcgccgt ttcaacaaac tattaaaaga atttgattcg atccgagaaa tagcaaaaga
    85501 agatcattgg gattatcatc atcaaaaagc accggctgaa tctttagtaa aggcaggtta
    85561 tcaattaagc gaaaaaatta aagaatggct ggcagaattt cctgagcacc catcacaaga
    85621 acaattgctt ccttattatt ttgatttgct tcattttttg aaggtcagtg aattttatga
    85681 tgatcactac gaaacaacag tcgaaaaaac gtatcgcgat ctgatagtaa aagagttttg
    85741 tattgatcca agcttgtttc ttgagcagtc gttggataaa ggaaaaagca gtattctttt
    85801 ttctgctagt ttttcaccac tctcttatta tcaggaaaca ctggggggac aaaaaagtct
    85861 ggcttacaaa ctgccgagtc ctttcccaga aggaaaccag caagttttca tcgcaaatta
    85921 cctagagacg acttatcgaa aaagggaaca cagtctggca aaattagtag agacgattca
    85981 tgcttttgct gatggaaagg tcgggaatta tttcgttttc tttccttctt atcaatattt
    86041 agatcttgcg gtggaagcat tcagacagca atatcccgac agcaatgtgc taatccaaga
    86101 aacggacatg aatgaggaag aacgcgaacg atttttaaca aaatttcaaa caaatcccgc
    86161 tgaaacattg attggatttt gcgtacttgg cgggattttt tcagaaggaa tcgatttgag
    86221 aggggaccga ttgatcggca gtatgatcgt aggtgtaggt ttgccgcaga tgaatcatga
    86281 gcaggaactg atcaaatcat attatgatga gaaggaacac cttggatttg cttacgctta
    86341 tcagcttcct ggaatgaaca aagtcttgca agctggtggt cgagtgattc gtggcatggc
    86401 ggatcaagga atcgttgtat tggcagatca acgattttct agtttttctt atcgacgttt
    86461 attcccacaa cattggcaaa cagcaagaga agtacgaagt gttggggaat tggaagaagg
    86521 aatacagcgt ttttggttat caaaagaagc agtaaatcaa aaaaacgagg aatgacagac
    86581 gtcagcctcg ttttttgatt aacgatagtt agtgaattgc aactcgattg gtaagtcaag
    86641 tttacgtaat aaagcaatca cagcttgtag atcatcacgg ttcttacctg tcactctaat
    86701 ctgatcttct tggatttgtg cttttacttt cagtttttca tttttgatag ctgtagtgat
    86761 tttcttcgcg ttttcacgat cgattccgtt gaccaaatcg gcagtttgtc gagctttgcc
    86821 acctagagcg tgttctgatt cggaaaaatg gatatttttg attggcacat ttcttttgat
    86881 cagcttacca aataatacat ctttgatctg ttcgattttg aaatcatcat cgctcaatac
    86941 aactagattg ctgttctcta atttgatttc tactgtagaa tccttgaagt caaagcgatt
    87001 ggacaattct tttgttgttt gctgaatcgc attttttact tcttctgtat tgacttctga
    87061 tacaatgtcg aagctagcat cttttgccat aggaaccctc ctgatataca tataaatatt
    87121 taccatacat ctatcttatc ataggattag ggcagattct tacctgtttg ctaaaattct
    87181 tctcatttga gttcgaagta ttttatttta atagataaac tgttattctt acatttggaa
    87241 taccaaagta ggggagaggt gcttgtgagt aaaattatga ctgcgtatca taaaaatgaa
    87301 agattaaaga aaatagtggt tattttgata accggattga ccgctgcggt cgctttaaat
    87361 tattttttga ttccagccaa tgtcttttct gcagggatga atgggatcgc tcaaatcatt
    87421 tcctcgcttt tatatgaatg gtttcatatc aagatagaca caggaacatt tatttttctt
    87481 ttgaatatcc ctgtgtttgt attagggttt ctcaaagtag gaaaacgagc aacgatcctt
    87541 agttttgtga atgttgtatg tgtatcggtg atgacgatgc tgcttccagt cggacagatc
    87601 actgataata ttttgatgaa tgccctagta ggcggtgttt tattaggtct tggcgtagga
    87661 ttatctttaa aaatgggctt tacaactggt gggatggata tcatctctct tgtactttca
    87721 caaacaactg gaaaaacagt cggaaactac atgctgattc taaatggtat cattgtagtc
    87781 atcgcaggct tcttctttaa ctgggaaagt gccttgtata cgattatttc catttatgca
    87841 atgactcaag taattgatgc catccacacg agtcatcaaa aagtaacagc aatgatcgtt
    87901 accgtcaagc cagaagaggt aacagaagaa ctagcaaaac ggatggtacg tggaatgaca
    87961 ttactgccgt ctgtcggtgg ctattcaaaa aaacaaggga acatgatcat gatggtcatc
    88021 acccgctacg aaatgtatga tcttgatcag atcgttcatg aaatcgacga cgatgcattt
    88081 atcaatattt taccaactca ttcagtattt ggccggtttg caaatgaaaa cgaacaaaaa
    88141 atgtatcgaa caactggcgt attgccagaa ataaaaaatc gttcacaaaa aaaatgagga
    88201 atcaggttta ttttagaata aatttaatct attacatcta ataagaacat ttttataaat
    88261 ttaaacgaga atcttacttc taaattctca tttacaagca gctaaaaaaa gagtatgata
    88321 aataaaaatc gtgttaagga gtgtcgtacg atgagcgaag caaaccaaga acggtctgga
    88381 caagaaatag aactaatcaa agaacgtatt cttgctgcct tggaaatggt gattgatcct
    88441 gaattaggaa tcgacatcgt caatttaggc ttgatttatg atatagaatt caatcctgaa
    88501 aatggagaaa cagtcattaa gatgactcta acaactatgg gctgtccatt agcagatatt
    88561 ttaacagaat ctatccatga cgcattaaaa gaagtgccag aagtaacgaa agcagaagtc
    88621 aaactagtct ggtatccagc atggacgaca gataagatga gccgttatgc acggattgcg
    88681 ttggggatac gatagaaatc ttggctgact agctatgaga agcaaatagg aaaaacaaaa
    88741 aagcctttta tcctttctag aagaaaagag ataaaaggtt tctttgttta taaaattttg
    88801 tctgctatat tataagttaa ttaaataaat gtgttctatt gataaaggac attcaaatcc
    88861 ctttcctttt ggaatccttc ttttttacat cagtttgatt ttcgtatagt attctttctc
    88921 atcttcacga taaatcgcag ccccattttt ttgttgtgct ttaagactgc caagattttt
    88981 tttatttaca gacaaaagtg cctcatcaat gccgattttt tttgcctctt gtagtactaa
    89041 tgctaatcct tgtgtggcat atcctttttt acgataatta ggggagatgc cgtaaccaat
    89101 atggccagcg ccgtttctta atgtttcgtt cagtttatgg cgcaaattaa aaattcctac
    89161 atactcatgt tcatcacttt ctaaaataaa gacggttgcg ggaacgtaac tctcaggaag
    89221 atcgattcct aaagacgttt tttcgcattt ttttacataa tctaaaaatt cagcaaatgt
    89281 atagccataa gcattgttga taaatccact ctcatttggt ccaaagctag tttgtagctg
    89341 ataagctttc tttaaatctg cttcccatag tttgatcagt ttcatcgtat tcacctcttt
    89401 aagtaacctt attcatttgt tttctactat agcactattg ttgtaaatag ccatgcctat
    89461 ttttatttat tgtgtcatat cgtattaaga atcatcgaac aatacaaata tgaattacaa
    89521 taaaaaaatt atttgaagtt cgttcattcg ttacttatga tctctagaag aaagaaaacg
    89581 tgaattactt cagcgtctag aattccagaa ggatagtaat caacagaaga aataatcaat
    89641 atccaaatga aagaagatag ctgaatcgag aaaaagagga aaaaagaaat gaagaaaaaa
    89701 ataaaatagt tactaatccc agagtcgttc gtttaacatt gaaatcgatc atatggataa
    89761 aacagatttt ttcaccaacc ctcaatagat gatggagttg ctcggatgaa ttatttgtgt
    89821 agatggcata agggagggcg tacagaaatg aaaaagaaca aaaatatgtt cttgacaaaa
    89881 aacacatgta atcctaaact aaaagatgtg atgcggctat ggcggaattg gcagacgcgc
    89941 tagcttcagg cgctagtgag ggtgacctcg tggaagttcg agtcttctta gccgcataat
    90001 ttgttttttt ataaagagaa ggtacacctg gagcgatgag aaagaaaata acatattatc
    90061 taaaagaata taggaaaaat tttaaacaac ggaaccagat tcaaataaat caaaaaaaat
    90121 ttgtgtgaag gtgggacagt ttaaaaccta atagattgat tcaaatagta gatttataga
    90181 ttctgataca agaaatactg gcaaacgata gttaaggaat aaagactaaa cttaaactca
    90241 aaaagaggtc atgcgaaaga ataaatcgca tgacctctta ctatattttg aatgtgttta
    90301 aacgttcaaa taatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt
    90361 cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag
    90421 agtagtcacc atttaagtaa gaagaatcta attggtaacg gccgatgtaa cgtccgttcg
    90481 ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtac
    90541 ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg
    90601 ctgcttgtgt ttccgtttga accgtttctt gtacaacagg ttgttcagct tgaacaggtt
    90661 cagcttggac tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc
    90721 cttctgttgg gattgttaat tgttgtcctg agta
//
LOCUS       DAGCXG010000003        78569 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGCXG010000003 DAGCXG010000000
VERSION     DAGCXG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.003"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(9..1644)
                     /locus_tag="MB022_000168"
                     /pseudo
     CDS             complement(9..1644)
                     /locus_tag="MB022_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1967..2446)
                     /gene="rlmH"
                     /locus_tag="MB022_000169"
     CDS             complement(1967..2446)
                     /gene="rlmH"
                     /locus_tag="MB022_000169"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBT4465198.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2874..4163
                     /locus_tag="MB022_000170"
     CDS             2874..4163
                     /locus_tag="MB022_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBT4465199.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4202..4807)
                     /locus_tag="MB022_000171"
     CDS             complement(4202..4807)
                     /locus_tag="MB022_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBT4465200.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5012..5476)
                     /locus_tag="MB022_000172"
     CDS             complement(5012..5476)
                     /locus_tag="MB022_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4465201.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5498..5818)
                     /locus_tag="MB022_000173"
     CDS             complement(5498..5818)
                     /locus_tag="MB022_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4465202.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5962..7041)
                     /gene="pepA"
                     /locus_tag="MB022_000174"
     CDS             complement(5962..7041)
                     /gene="pepA"
                     /locus_tag="MB022_000174"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBT4465203.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7241..7591
                     /locus_tag="MB022_000175"
     CDS             7241..7591
                     /locus_tag="MB022_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4465204.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7814..8761
                     /locus_tag="MB022_000176"
     CDS             7814..8761
                     /locus_tag="MB022_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4465205.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8818..10236)
                     /gene="pepV"
                     /locus_tag="MB022_000177"
     CDS             complement(8818..10236)
                     /gene="pepV"
                     /locus_tag="MB022_000177"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBT4465206.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10385..11230
                     /locus_tag="MB022_000178"
     CDS             10385..11230
                     /locus_tag="MB022_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBT4465207.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11234..11674
                     /locus_tag="MB022_000179"
     CDS             11234..11674
                     /locus_tag="MB022_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBT4465208.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11724..11918
                     /locus_tag="MB022_000180"
     CDS             11724..11918
                     /locus_tag="MB022_000180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465209.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12054..12740
                     /locus_tag="MB022_000181"
     CDS             12054..12740
                     /locus_tag="MB022_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4465210.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13148..13837)
                     /locus_tag="MB022_000182"
     CDS             complement(13148..13837)
                     /locus_tag="MB022_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465211.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13812..15941)
                     /locus_tag="MB022_000183"
     CDS             complement(13812..15941)
                     /locus_tag="MB022_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBT4465212.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15989..17326)
                     /locus_tag="MB022_000184"
     CDS             complement(15989..17326)
                     /locus_tag="MB022_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBT4465213.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17578..17937)
                     /gene="rplT"
                     /locus_tag="MB022_000185"
     CDS             complement(17578..17937)
                     /gene="rplT"
                     /locus_tag="MB022_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBT4465214.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17990..18190)
                     /gene="rpmI"
                     /locus_tag="MB022_000186"
     CDS             complement(17990..18190)
                     /gene="rpmI"
                     /locus_tag="MB022_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBT4465215.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18262..18786)
                     /gene="infC"
                     /locus_tag="MB022_000187"
     CDS             complement(18262..18786)
                     /gene="infC"
                     /locus_tag="MB022_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBT4465216.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18830..18955)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19106..19738
                     /locus_tag="MB022_000188"
     CDS             19106..19738
                     /locus_tag="MB022_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4465217.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19740..20645)
                     /locus_tag="MB022_000189"
     CDS             complement(19740..20645)
                     /locus_tag="MB022_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4465218.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20730..21389)
                     /locus_tag="MB022_000190"
     CDS             complement(20730..21389)
                     /locus_tag="MB022_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4465219.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21705..22649
                     /gene="mvk"
                     /locus_tag="MB022_000191"
     CDS             21705..22649
                     /gene="mvk"
                     /locus_tag="MB022_000191"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBT4465220.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22642..23619
                     /gene="mvaD"
                     /locus_tag="MB022_000192"
     CDS             22642..23619
                     /gene="mvaD"
                     /locus_tag="MB022_000192"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBT4465221.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23632..24717
                     /locus_tag="MB022_000193"
     CDS             23632..24717
                     /locus_tag="MB022_000193"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBT4465222.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24714..25778
                     /locus_tag="MB022_000194"
     CDS             24714..25778
                     /locus_tag="MB022_000194"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBT4465223.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26027..26830)
                     /locus_tag="MB022_000195"
     CDS             complement(26027..26830)
                     /locus_tag="MB022_000195"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBT4465224.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26850..27692)
                     /gene="kduI"
                     /locus_tag="MB022_000196"
     CDS             complement(26850..27692)
                     /gene="kduI"
                     /locus_tag="MB022_000196"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBT4465225.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAETPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27708..28715)
                     /locus_tag="MB022_000197"
     CDS             complement(27708..28715)
                     /locus_tag="MB022_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBT4465226.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28722..29366)
                     /locus_tag="MB022_000198"
     CDS             complement(28722..29366)
                     /locus_tag="MB022_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBT4465227.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29466..30224)
                     /locus_tag="MB022_000199"
     CDS             complement(29466..30224)
                     /locus_tag="MB022_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBT4465228.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30351..31502)
                     /gene="fucO"
                     /locus_tag="MB022_000200"
     CDS             complement(30351..31502)
                     /gene="fucO"
                     /locus_tag="MB022_000200"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBT4465229.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31584..31895)
                     /gene="rhaM"
                     /locus_tag="MB022_000201"
     CDS             complement(31584..31895)
                     /gene="rhaM"
                     /locus_tag="MB022_000201"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBT4465230.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31897..32742)
                     /gene="rhaD"
                     /locus_tag="MB022_000202"
     CDS             complement(31897..32742)
                     /gene="rhaD"
                     /locus_tag="MB022_000202"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBT4465231.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32756..34012)
                     /gene="rhaA"
                     /locus_tag="MB022_000203"
     CDS             complement(32756..34012)
                     /gene="rhaA"
                     /locus_tag="MB022_000203"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBT4465232.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34009..35469)
                     /gene="rhaB"
                     /locus_tag="MB022_000204"
     CDS             complement(34009..35469)
                     /gene="rhaB"
                     /locus_tag="MB022_000204"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBT4465233.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35622..36491
                     /locus_tag="MB022_000205"
     CDS             35622..36491
                     /locus_tag="MB022_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBT4465234.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36617..37516)
                     /locus_tag="MB022_000206"
     CDS             complement(36617..37516)
                     /locus_tag="MB022_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465235.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFFSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37681..38505)
                     /locus_tag="MB022_000207"
     CDS             complement(37681..38505)
                     /locus_tag="MB022_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4465236.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38516..39811)
                     /locus_tag="MB022_000208"
     CDS             complement(38516..39811)
                     /locus_tag="MB022_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBT4465237.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39827..40306)
                     /locus_tag="MB022_000209"
     CDS             complement(39827..40306)
                     /locus_tag="MB022_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBT4465238.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40312..41340)
                     /locus_tag="MB022_000210"
     CDS             complement(40312..41340)
                     /locus_tag="MB022_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBT4465239.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41357..42463)
                     /locus_tag="MB022_000211"
     CDS             complement(41357..42463)
                     /locus_tag="MB022_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4465240.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42692..45289)
                     /gene="adhE"
                     /locus_tag="MB022_000212"
                     /gene_synonym="adhC"
     CDS             complement(42692..45289)
                     /gene="adhE"
                     /locus_tag="MB022_000212"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBT4465241.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45594..45974)
                     /gene="yajC"
                     /locus_tag="MB022_000213"
     CDS             complement(45594..45974)
                     /gene="yajC"
                     /locus_tag="MB022_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBT4465242.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46071..47216)
                     /gene="tgt"
                     /locus_tag="MB022_000214"
     CDS             complement(46071..47216)
                     /gene="tgt"
                     /locus_tag="MB022_000214"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBT4465243.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47434..48132
                     /locus_tag="MB022_000215"
     CDS             47434..48132
                     /locus_tag="MB022_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4465244.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48300..50939)
                     /locus_tag="MB022_000216"
     CDS             complement(48300..50939)
                     /locus_tag="MB022_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4465245.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51558..52589)
                     /gene="queA"
                     /locus_tag="MB022_000217"
     CDS             complement(51558..52589)
                     /gene="queA"
                     /locus_tag="MB022_000217"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBT4465246.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52782..53957
                     /locus_tag="MB022_000218"
     CDS             52782..53957
                     /locus_tag="MB022_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4465247.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            53961..54602
                     /locus_tag="MB022_000219"
     CDS             53961..54602
                     /locus_tag="MB022_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465248.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54599..55516
                     /locus_tag="MB022_000220"
     CDS             54599..55516
                     /locus_tag="MB022_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4465249.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55513..56184
                     /locus_tag="MB022_000221"
     CDS             55513..56184
                     /locus_tag="MB022_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465250.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56725..57213)
                     /locus_tag="MB022_000222"
     CDS             complement(56725..57213)
                     /locus_tag="MB022_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBT4465251.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57414..57680)
                     /locus_tag="MB022_000223"
     CDS             complement(57414..57680)
                     /locus_tag="MB022_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBT4465252.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57680..57943)
                     /locus_tag="MB022_000224"
     CDS             complement(57680..57943)
                     /locus_tag="MB022_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4465253.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58419..58604
                     /locus_tag="MB022_000225"
     CDS             58419..58604
                     /locus_tag="MB022_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4465254.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(58746..60569)
                     /locus_tag="MB022_000226"
     CDS             complement(58746..60569)
                     /locus_tag="MB022_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBT4465255.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61167..63872)
                     /locus_tag="MB022_000227"
     CDS             complement(61167..63872)
                     /locus_tag="MB022_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBT4465256.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64178..65125)
                     /locus_tag="MB022_000228"
     CDS             complement(64178..65125)
                     /locus_tag="MB022_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBT4465257.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65315..65584
                     /gene="rpsN"
                     /locus_tag="MB022_000229"
     CDS             65315..65584
                     /gene="rpsN"
                     /locus_tag="MB022_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4465258.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65760..66122)
                     /locus_tag="MB022_000230"
     CDS             complement(65760..66122)
                     /locus_tag="MB022_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4465259.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66133..67254)
                     /locus_tag="MB022_000231"
     CDS             complement(66133..67254)
                     /locus_tag="MB022_000231"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBT4465260.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67270..67620)
                     /locus_tag="MB022_000232"
     CDS             complement(67270..67620)
                     /locus_tag="MB022_000232"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBT4465261.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67750..69261)
                     /locus_tag="MB022_000233"
     CDS             complement(67750..69261)
                     /locus_tag="MB022_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4465262.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69583..70935)
                     /locus_tag="MB022_000234"
     CDS             complement(69583..70935)
                     /locus_tag="MB022_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBT4465263.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71088..72164)
                     /locus_tag="MB022_000235"
     CDS             complement(71088..72164)
                     /locus_tag="MB022_000235"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBT4465264.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72360..74105
                     /locus_tag="MB022_000236"
     CDS             72360..74105
                     /locus_tag="MB022_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBT4465265.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74385..74822)
                     /locus_tag="MB022_000237"
     CDS             complement(74385..74822)
                     /locus_tag="MB022_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBT4465266.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74806..76179)
                     /locus_tag="MB022_000238"
     CDS             complement(74806..76179)
                     /locus_tag="MB022_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465267.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76501..76989)
                     /locus_tag="MB022_000239"
     CDS             complement(76501..76989)
                     /locus_tag="MB022_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4465268.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77011..77613)
                     /locus_tag="MB022_000240"
     CDS             complement(77011..77613)
                     /locus_tag="MB022_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4465269.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(77682..>78569)
                     /locus_tag="MB022_000241"
     CDS             complement(77682..>78569)
                     /locus_tag="MB022_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBT4465270.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT        22618 a        16696 c        14316 g        24939 t
ORIGIN      
        1 aaatgtaatc agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa
       61 agaaagaggt aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg
      121 gaagaaactt caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc
      181 aatctctctt ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag
      241 cgttattttt tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg
      301 ataaggcata gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt
      361 gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta
      421 acgaataaga ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc
      481 atagtttgct tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt
      541 aactactttt ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga
      601 gccaaacagt acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt
      661 attttgtaat aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact
      721 aaggtaaatc actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa
      781 agaaataata ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat
      841 caaactatcc gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat
      901 cgattgaacc atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt
      961 cattcctact gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc
     1021 acttactaca aagccgccta agactagtaa gccaacggct attacactca taaaagtgga
     1081 aagaacatgt gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt
     1141 tttctcataa atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat
     1201 cgcttcagca atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata
     1261 taaacagcta aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa
     1321 catcagcatg atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa
     1381 attattactt atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata
     1441 attcactccg ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga
     1501 agataaccct ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg
     1561 aacatttcta aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa
     1621 tattattttt ttctttactt gcattactta attatatact cctttcatgg attgctttat
     1681 ctagtataac ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca
     1741 ctaaaattat aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat
     1801 tgcttaacta atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt
     1861 gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg
     1921 gatatgacta tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag
     1981 gcgcccccgc attgatccgg aaggcacggt agatttgctc gacaaggatc agacgcatca
     2041 attgatgtgg atacgtcatt tttccaaaag agatctgagc attgcttctt ttcatgacgg
     2101 cttcgctgag tcctaaagag ccgccgataa caaaaacaag gtggcttttt ccttggatac
     2161 ctaattggtc gatttgttta gcaaaagatt cacttgtagg atttttccct tcaattgcta
     2221 aggcatagac gtattcttgt tctttgattt tcgctaagat ccgttcccct tctttttctt
     2281 ttacttgtct catttgcgct tcgcttaaat tctccggggc tttttcgtca ggaacttcga
     2341 tcaattcgat ttttgcatag gcattcagcc gtttgacata ctcattgata ccttggatca
     2401 ggtatttttc ttttaatttt cctactgaga taattttgat attcatgttt tctctccttt
     2461 ggtcttcatg ctcactttgt ttattataat caattttttc gtctatatgt ttattttggc
     2521 tatttttgtt aaaaagattt ccccaacagt tatgcacaac atccacaggt ttatccacag
     2581 aaaacagtgt tattttacct tatttcatca cattcattag gcaaacatac gttttataag
     2641 aatttatcca ctttatacac aggctgtgga tatgttttat cccttatttt tccaatccta
     2701 tccgcgtagt tttacacagt atctgtggaa aaagaaaatc aaatattttc atctgaaaac
     2761 aatgattttt cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa
     2821 caaataagac tatactaaca gtagataaat gaatgtaatt ggaggaggaa cacatggata
     2881 gaaaaaatgt gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag
     2941 gtctggggct tgttggaggg atcgttggag gactactgac agctggcatt ttttatgcag
     3001 cgatgggcac tggcaacaat agttctactg ctacaagcgg caatcagaat tctgccggtg
     3061 aaacagttgt ggaaaatgtc aaagtcaatg tagattctga tatcaccaaa gcggtagata
     3121 aagttcaaga cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa tcaagcggtt
     3181 ttggcggact attcgggcag caagaagaaa gtagcagctc agatgatagt aatttggaag
     3241 cttatagtga aggaagcggc gtgatctaca aaaaagacgg gaatactgct tatgtagtca
     3301 cgaataatca cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa gacggtacga
     3361 aggtaaaagc tgaattagtt ggaacagatg cttattcaga tttagcggtg ttgaagatca
     3421 attcagacaa agtcgaaaca gttgcctctt ttggtgactc aagtgctctg aaggttggtg
     3481 agcctgcgat tgcgattggt tccccactag gatctgaata tgcaaattct gtcacttcag
     3541 gaatcatttc ttccttgaac cgacaagtga caagtacgaa tgaatcgaac gaaacagtca
     3601 atatcaatgc gatccaaaca gatgcagcaa tcaaccctgg aaattctggt ggtcctttag
     3661 ttaatatcga aggacaagtt atcggtatca actcaagtaa gatcgcaagt acttctgaat
     3721 ctacttctaa tgtaagcgta gaaggaatgg gatttgctat tccaagtaac gatgtagtga
     3781 atatcatcaa tcaactggaa aaagacggaa aagtgacacg tccagctcta ggtatcacaa
     3841 tggtcgactt atctgccgtt tctactcagc aacaagaaca aatcttgaag attccttcat
     3901 ctgtaactaa cggtgtcatc gtgacaaatg tccaaacagc tacaccggct gaaaaagctg
     3961 gattgaaaca atacgacgtc attactaaga tcgatgacaa agaagtttct tctggtgtag
     4021 aacttcaatc tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc acttactatg
     4081 aaggctcgaa agaaaaaaca actactgttg aattgacgat cgatcaatcg gcattaaaac
     4141 aaaatcaaaa tagtgaaaat taataaaaaa gaggttgtga caaagacttg tcacaacctc
     4201 tttattttgg aaatgcttct ccgactacgg catcctcagg caatactaag attccttttt
     4261 ttgctggagc atttggcaaa tgcaattcac gagcggaaca gatcattcca tagctatcta
     4321 cccctcgcaa agttcctggc cagatcatca ttccatctgg catcatagct cctggttttg
     4381 caacgacaac tttttgtcca gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg
     4441 taccattgtc tacttctgtc tcagtaatcg ataaatggtc agaatcagga tgtgggacgc
     4501 atgtttttac gtacccgaca acgatcttcg gttcatgatc agcttcaagc tgttcttcga
     4561 atcctgcttc tttgatcaat cgattcaatt tcgctaattc ctcatctgtt aagcttactt
     4621 ggccgttccc ttcgattgtt agatgatcgg agatatggaa aaagttccaa gcaacaactt
     4681 gaccttgttc atctttgatc cgagcaacat tccatttgcg ttcaacagtg ttttctgttc
     4741 ctttatcgtc ggctataata accatcaagg tgtctccgac gtgtgctttg ttataagcaa
     4801 aaatcatgtt ctttcctcca ttatcctttg ctgagattta tcttaacaaa aaattgcatt
     4861 cactgcatga aatttttttc atgctttatt tccgaagaag cctcttctgg aacaccattt
     4921 attcgtaaca agggactgtg acaagacttt tgtcacagtc cctttactgt tttagttaat
     4981 tgattaaaaa ggtcgtacaa gttccacttg cttaattact tggctgttct tcttcgtcag
     5041 aaacgatcaa gacatcacaa ggtgaatgac gtatcacgta gctggctaca cttcctgtca
     5101 taaagcgttc gactgcattt agacctgact gcccaaccat gattagatcg atctcatatt
     5161 tttttggcaa tttgactgcc atggattcac gaacagaacc atatgcaata attccttcta
     5221 tatttgtgta atcaacagat ttcccatatt ctttacattc ttcgatcatt tccttggctg
     5281 tttcagtttg ctgatcgact agattttgat caataggtgc atagcccata aagttataaa
     5341 attgctgatc gattacgttt gctacatata ctttcccatt attacggcga gccacttcta
     5401 ccgctttttt gaaagcagca agtgcttgtt cactaccgtc gatacctact aaaatattct
     5461 tatattcttg attcatcttg acccctccta acagatgtca gccttttgtt acactttcta
     5521 ggaacgattc gatttcctgt tttgttttac gatctttatt cactagacga cctaattctt
     5581 ttccgtcttg gatcacgaca aaacttggga taccaaaaat gttccattca gcggctacat
     5641 ctatgtactt gtcacggtct acttcaataa attgccaatc agaaaagtca ttttcaattt
     5701 caggcatttg cggtttgata aaacggcagt ctccgcacca gccggcagta aagaaaaata
     5761 cactctttcc agtttctacg tagcctgcta attcttctaa actatttggg ataatcattc
     5821 tttgtcctct actttctgtt tatgtatttt tcaattaaat tatacacctt tctttcgaac
     5881 taacaaacat tgatcctctt tttataaaaa aaatctggca aaaagcaatc ttttgccaga
     5941 ttttatagtt tcactatcgc attattttcc gtatacgatc gtatttactg ttgatttatc
     6001 caaacctttc agtgtttgaa tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa
     6061 catcgtttga tgcgtatgga tataacgtcc cactacgcca atcactgtac ttgggattcc
     6121 ttcgttttgt gtatgagcag cacctgcatc tgtaccgcct tttgaaacaa agtattgata
     6181 agggatattg tttgtttcag cgatatccag caagtactcg cgcaaacgag gaagcatgat
     6241 caaacctgga tcttggatac gcatcaatgt accttctcct aaatgtccga atgtgccatt
     6301 tttcgtcact gtatcatctg ctgcagagca gtctactgca aagaacagat ctggcttgaa
     6361 tttattgaca gaagctttag aaccacgcaa gcctacttct tcctggacat tcgcgcctgc
     6421 aatcaatgta tgacctaatg tttcattttg caacgcttct aaagcttcta ataccattgt
     6481 acagccatag cggttgtccc atgatttact gataatattc tttccgttgg ctgttttgat
     6541 cgtttcagcg taaggaacga ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc
     6601 tcttgattca aacccagcat cgaacaaaat atcggttact tctactgatt tttgtccgct
     6661 tgttcctcgc aataaatgtg ggggaacgga agaagaaata catggataat tcttaccagt
     6721 agaggtttta agtgtaaaac gctgagcaga aacaacgtaa ggattccagc cgcctagagg
     6781 tacgacttta aaaagcccgt tttcttggat ttgtgtcagc atgaatccga cttcatccat
     6841 atgtgctgcg accattacac ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat
     6901 gccgcccaaa ccatctaact gtaactcatc taccagcggt tccatatgac tttccatata
     6961 agcgcgaaca tcttgttcaa aaccgcttgt tccttgtaat tctgttaact ctttgattcg
     7021 ttgaaatgtt ttttcttcca tatgctattc gtctaaattt cagactaaac tcctctcaga
     7081 cttatattga tctgcggata aaatatgatt ttttctgcag accgatcgta tttattatag
     7141 cttatttttt ctggtagcgg ttatttattt caaagaaaat atgagagtgt ggtaaaataa
     7201 gcacaaggag taacaagtga gaagaaggag gtatgtccac atgtatgaag aaagcgaact
     7261 aagctatttt aaaggcgggt tagcagttgg aatcggtcta ggattattga gcggggtagc
     7321 ttcaacgatt tggtatcatc gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat
     7381 caagtcatct tttctaaaag aaggtgcaat cgaaggttct tggatctctt ttgaaaagga
     7441 acctattagg aaattcgccg ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga
     7501 agatggcgaa actgtttatt atgaattttt agcagatgct catacaggaa ccgtattaga
     7561 aatttcaaga aaaaaaggtg caacagatta atacgtcaaa aaagtgagag aacgaatacg
     7621 cacccactat tcaaaagaga ggtatcatgt ccttcttttg tagtgggtgg ttttttagat
     7681 agagctttcc aaacatatat tccagtattt tctttttaaa taaatgatat ttagcgggaa
     7741 aaactagcat ttcctttgat tatgaggtat ttttaattta gcttttgttt tctatagtta
     7801 aggagagtga aaaatggaac aacaagaaaa aattgctgtt ctgattcctt gttacaatga
     7861 ggaagcaaca atttcaacag tcattgcaga ctttaaacga gaattaccag aagcagatat
     7921 ttatgtctat gataataatt cgaccgatcg aacatacgaa ttggccgttc aaggtggagc
     7981 aatcgtaaaa aaagaacctc gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga
     8041 tatcgatgcc gactattatt taatggtaga tggcgatgat acttatcctg cagaagctgt
     8101 acatggtttg ctcgagaaat tacgttctgg tgaagcagat atggtcattg gtgatcgcct
     8161 atcaaatgga acctactttg atgagaataa gagacctttc cacgattttg gaaataacct
     8221 tgttagaaat acaattacta gaatgtataa aacaaaaatt cttgatgtca tgaccggcta
     8281 tcgaggattc aatcgtatct ttgtcaaaag ttttcctatt atgagttctg gttttcaaat
     8341 cgaaacggaa ttgacgatcc atgctttaga taaaaaattc aagttcgttg aaatcccaat
     8401 cgattatcgt gatcgtcctg aaggcagcga atcaaaattg aatacattct ctgacggatt
     8461 caaagtgatc atgatgatta ttaaaatgtt taaagattat aagcctttgc ttttctttgg
     8521 gatttggacc atcttcttct ttttatttgg attggtcacg gggattcctg tgattcgcga
     8581 atatatgctg acaagcttta ttacacgcat cccttctgcg attttaagta ctggattgat
     8641 gactttagca cttctttcat tagtaacagg gttgattttg gatacagtcg tcacgaacgc
     8701 taagaaagaa tatgaattga atctatatca tgtttatcat gaataccaca gtagaaagta
     8761 aaaaaaggat caagactgca aaaataattt gcaattcttg atcctctttt taattaatta
     8821 tttcgccaaa cgatagatgg catctgcata gattgctgtt gctctgaaca agtcatcaag
     8881 actcataaat tcgtttgctt gatgcattgt atcagtatat cctgggaaca tcgctccata
     8941 agcaacgcca cgtttcaaca aacgtccgta agttccgcca ccgatgattt gttcgtagcc
     9001 tttttctcca gtgtggtctt catagacttg caataatgta gccaccaatg gatcatccat
     9061 tggtacgtaa tgtggttcca ttacgcgagc acctcgagta actgttgcgc cttcttgccc
     9121 cactgtttgt tcgatgtcac tttgcaatcc gtctactgtt acaccttttg gaaaacggaa
     9181 attcatattg atgaagttcg cttcctctgt accattttct tcaaaagcaa atagtccagc
     9241 attcattgtc aatttgccca ttttttcatc ttcataagcg actcctagtt tttctccata
     9301 aaaatcttca tggacataag cagctgctac atggataaat tgtttagcag atcctaggaa
     9361 ttcatagtta tctaagaaca atgccaagaa agaacctgca ttgataccag attgtgggct
     9421 tgcaccatgt gcgcctttac caactaattc gatttttaca tacgtattgt ccgcttcaat
     9481 cgttccgctt actggatttg cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc
     9541 tgcatctgca cttggtactt gaagtgctgc agttgctgtt cctggcacca tattttcgcg
     9601 aagtccggaa acaaaagatt tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa
     9661 agctaaagaa acatttcctt tttcaccatt gatgattggg aattcagcat ctggtgagaa
     9721 accaaaatct ggttcttctt catgttcgaa ataataagcc atatcgcccc agccgctttc
     9781 ttcatcgctg ccgactacga agcgaacttt ttttgatagt ttcaagtcaa gttctttaat
     9841 gatcttcatc gcatagtaag ctgccatact tggacctttg tcatcgcttg atccgcgtgc
     9901 aaagatcttt ccatccttga ttactggttc ataaggatct gtgtcccagc catctcctgc
     9961 aggaacgaca tccatgtgtc caaatatccc tagcgtttcg tctccttcgc caagatcaat
    10021 atgccctgca tagttatcga cattttttac tttaaatcca tcacgttcgc cataagctaa
    10081 catatgtttc aatgcgtcgc gaggtccagg tccaaatggt gcatctggtg ttacttttga
    10141 gtcatcgcgc tcgctgttga cacgtaaaag atttttcaag tcttcaagca agtcttcttt
    10201 acgtgcttct acttcttttt gccaatcaat tgtcataaag ttaatcctcc acttatttaa
    10261 gtcttcgtgt tcatgatacc acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt
    10321 ccttttctta tctgtttttc tctaaaaaaa atatacgcta taatgagtaa aaggaggttt
    10381 ttagatgctg ataaacgaac aactagtgaa aagagtcatc acacctcgtc caaaaatttc
    10441 acataaagga acatttggac gtatcgtttt gattggcggc aatgaacaat atggcggagc
    10501 aatcattatg agtgctttag ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac
    10561 tgatgaaaaa aatcggagtg ctcttcacag tcatctgcca gaagcaatga ttgtagtttg
    10621 gacggatact gaccagtttc tttccgtaat aaagcaagca gatgtactct tgattggtcc
    10681 cggattgggt acttcttcta agagtttgga acgtttcaaa caagtactag aaaaccaatc
    10741 aaaagaacaa tggctggtga ttgatggctc tgctttaagt ttacttgctg ctaatcatct
    10801 tttgctaccc tatcccgaac aaactgtact tacacctcat caaatggagt ggcaacggtt
    10861 gagttctcta ccgatacaag aacaacggga taaagaaaac aaaaagaagc aacaggaaat
    10921 cggtgctgtg ctcgttttga aaagtcatga gacaaaaatt tatggtcacc agcacttcta
    10981 caccaatcct ttaggttctg ctgctatggc aacgggcggc atgggtgaca ctttagctgg
    11041 gatgatcaca ggatttttag cccagtttcc tcaaaatgac caaacgattg cagcagctat
    11101 ttatcttcat agtttcattg gagaccagct ggcaaaagaa caatatattg ttttgccgac
    11161 acaaatcagc gaagcacttc cgaaatggat gaattatttt tgtaaaaaat caatggaaaa
    11221 cgaggcatga gtcatggaga aatttacagg ttatgtgcga gaaccttttt attatgaaac
    11281 agatcaaatg gggattattc atcacagcaa ttatatccgc tggtttgaag aggctcgggt
    11341 agccttattg gtacatctag ggtttcccta taaaaaaatc gaagaatctg ggatcatcat
    11401 ccccgttctg gaagtaaatt gcagctataa agaaatgatc cattacggag aaaaagtgag
    11461 catccatcca actatccaaa agtatacggg gacacggttg gattttagct acgaaattgt
    11521 gggaactgaa gacaaaaaat taaaaactac tggcagtagt aaacactgtt ttttactttc
    11581 tgattcgcaa cgattagtga agctgagcaa agtcaatccg gagttagatc agttattcca
    11641 agcgtacgct tctttggatg atgtaaattc ttaataaaga ccgtctaagc atttttcttg
    11701 tattgtgtcg agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg
    11761 ttaaagtaat gcacggttac atcgataaaa ctggctgtcg cactcgggaa aaaaacttag
    11821 acaatctgct cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg
    11881 gtcgtgtcaa accgatccaa gaggtacgac cagattgatt caatattttt cttttcgagg
    11941 ctctaatagg catgcctatc agggcttttt ttgcagactg tgtttttatg cccacacgat
    12001 ttgtaagcga tttatgttat aatagattat atctgcatag aaaggaaggt taaatggtta
    12061 tcaataattt gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt tattccgagt
    12121 tagcacctgg aagtaaaatc tccgataaag tgttagaaga acaactagct atcgggcgta
    12181 caccgattcg agaagcactg atccaactgc gcaatcaaga gcttgtaaaa accattccac
    12241 agtccggcac ttatgtttca aaaatcgata tccgatctgc ttcattagcc cgttatacgc
    12301 gggaaaagtt ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc
    12361 aagctaaact agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa
    12421 aagctttttt tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac
    12481 gagaaatttg ggattggcta gaaagttata gtacgcactt gaaccgtttc agatggttac
    12541 gtttgacgat ttcagaatta gactggggtc gagtattaga tgaacatcag acaatgcttc
    12601 aatcaatgat tgatcataac tttgatgaag tcggatttct ttgcacgatg catttacata
    12661 tgattatcga ggagcaagaa tacgtgattc ataattaccc cgattatttt gaagatattc
    12721 aggacagacc catcaaataa gaggttttgg aatcagcgtt ttgacctgag tattagagaa
    12781 agaaacggca aaatagttcc tcatattttg ccgttttttt tgagctaata ccgagggtca
    12841 gctgattgca caccgtttat tgagaggttg tgaaaaagct gccctgcatc aagtaataag
    12901 aacagccaag taaaaatagc ttcttatgtt tttaacttgg ctggacttat taccgcagat
    12961 gcaagctttt gaacaccgtt ttccaagagg tcgtgagcct gccgttcatc tccgagtcac
    13021 aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa tattttgact tgtgaccgag
    13081 gagttgacag gagaacatcg tttattcgga aaaaaaggac tgtgacacga cttttgtcac
    13141 agtctcttta tttgtaatct aaatcatact ccgtacatct tttttctatt ttttttattt
    13201 cttctaagat cccttggcta cgaatttcat tatatggcca aagtaagact gcctctaata
    13261 cataaggcga aaaatcgctt tgattcatct cttttgaaaa actcatcgct gctatctcgc
    13321 tcaatgcacg aatgtttgat tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt
    13381 cgctttgcgt ataaattccc ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa
    13441 taatcaaagc ttgcgctgtt tctttagaga tcgaagctag tcctgaacat tttaattttt
    13501 ctaacggctc cgtcatcaat cgaatccctt catcttctgc ataagttgca aaagtgattc
    13561 gttcgtttat ttgctctgat tcacaggaaa cgattggaac agccatttgt tcacaaattt
    13621 ctcctacatc ggcaaaaggg tacttttttt tgatgctaca tgccgtttcg ataccacaat
    13681 cgattgattg ttgtataaga attttctgct tggacaaagg aatctttttc caaaataaat
    13741 ctcgccgaaa ctggtagaat ccccaaattt cttcggggat ctgttgtaat tcgtttagta
    13801 actcttctaa cttatgtttg tttgatattt ccggcaacga cgatcacttc ttcctccggt
    13861 tccatagtag atacttccag atccatcaaa agctgtagtt gttccagatc cgtcgctgta
    13921 aaatcagaga ctcttgctcc taagcagaga taaaattctg gtcgggcaat caattcagtt
    13981 tgataaactg ccatatcttc ccaaagttgt tcgacagctg tcatataatt ggctgcagtt
    14041 gttttcatac atgaagcatg tccacgttgg accttgataa atcctttttg atcgatgatc
    14101 cgaagacttc cagcattttt tgaacgattc tgttcgccgc atacataaaa gaaaggtgta
    14161 ttttccaata agcaaacttc atttttattt aatcgcatat cctctgcagc aagctctagt
    14221 gcttcttctg ttgtgatctc cttggttaga tcggttgctt tcacttccgt agaaccagta
    14281 gcgatagccg ttacttttga agtctgtgga tcgatttcta cgtggacttc gatcgattct
    14341 ggtgtagcgc ctgattcaat cgctttattc atcgcttcat ttttcaacga tcgtatatct
    14401 tctttacttg gcgaagggat gatacgttct acaacatctc ggaccattgc aagtgctaca
    14461 ccaatagaag aaatcacttc ggcgttttca ggaatcgaat atttcacacc cattttatta
    14521 ctaaagtaag taatcaatga ggcagcgccg ccccctacac ctacaagaga aatctgatct
    14581 ttctccaagt gatacttgtc tgccaattct aaaatcaccg gttcaatctt agcataggat
    14641 ttttccatga tctgttctgc gatgtcttca accgtcgtat gacaataatc agctaatgct
    14701 tggatggctt ttcgtgcaga tgggacattt ccataagaaa aatgttcttc ttgcactaat
    14761 ccaaggacat tggctgcaca tgtatttgta atagtcactt cttctccgtc ttccatcact
    14821 accttgacgt aatcagcagg gtctccttct ttaggagaga aaaattcaac cttaggacct
    14881 ttgatttttt ctgtctccgt aaatactgca taatctagac cggcaatatg tgcagaacgt
    14941 ggaccgacat cgatgattcc tgactgatta gcccgtacca tcgaaccgcc tgccacacct
    15001 agaacccgaa catctaatga agagatatat gttgggtggc cacctacaat tgaataatcg
    15061 attgctggac gtccattttt aatgaccccg atattggttg ttgttccgcc tacttcaaaa
    15121 taaacaccat tagacgctct caagtacatc aaagagccca tgactgatgc tgctggaccg
    15181 gaaagcatgg ttaaaacagg tcgttttttc atttcattga tttccattac tccgccgtct
    15241 ccgcgcataa tcatcaaaga aacattgacc cctgcttctc gtacggaatc ttctgtagag
    15301 gttgccgtat ctagcatttt tggtaaaata gatgcattga ttgctgctgt ccgagttctt
    15361 cttgtcaatc catataattt tgtgatgtca gaagccatcg ttgtagggat cgattttacc
    15421 gatgctgctt cataaacaac tcgttctggt tccccattat ctactccaaa tgccatagaa
    15481 gaaaccaaga cctccgctct ttctctttct aaagaagaaa tcgtttctga aacacggtct
    15541 acatttaagt gtttgacagg taagaatgca ttgacaatct ctattttttt cttattccct
    15601 aaatcgatat cgttcaatct agtttgtcgt tttgctaaaa aaccttctaa tccacctttt
    15661 gccatcccga ttacgccgac ttttgctacg tctccttcaa tcagtgcatt agtcgcttgt
    15721 gtggtagaat gtgcaacaaa cacaacatct tctggactga tattattttc cagcaaacaa
    15781 ttttgaaaag actgaacgac acctgcggct actccacgga catcatcgtg ggttgttttc
    15841 actgaagatt ttccgatgat ttcgtgcgtc gcattatcga ttgcgactgc tttcgtatgt
    15901 gtaccgccaa catcaattcc cattcgcaca aaacgtttca ttttcttttc ctccattcat
    15961 ctaatcgttt ttatcgacgt tttgtttact aggcgttccc gtacaataag aacgtgatca
    16021 ggcataagat cgctccgatg atccaaccag ttgggatgga tttcttcata tattctcgag
    16081 aagttacttt tgtataacct aatccccaag caacccaaga ttgtgtaata tctaaatgct
    16141 gaggagcaat cgttgtgatt gcaaataacg ggaacaaaaa ggctggcgac atcgttggat
    16201 taacagctaa tacgacagct aaaatagctg agcctgaacc gactagattc attggaccgc
    16261 ggaaaaatcc tagtggtgtc aatatagcaa atacgattgc caagactaat ggtgattgcg
    16321 gtatccaatc tccaaaaata gctgaaaaat aaggagaagc atacgcagct gaattattga
    16381 acatcgctaa ggtcatcaag aaaccaacca ttggcgcaac atctactgcg ccatctgtaa
    16441 ataatttcgc aaactgacga cagatttctt tataacctcc gcgcaatttt ccagtaaaca
    16501 gcaatgccca gatacctgct agaataaaac ctaagatgat tggaagatct aaaagaacga
    16561 cccctaaaac tggtaataat acggctaacc atgagatcat tggtgcgtcg ccaccttcag
    16621 ctggtacgtt tgctgccatt gcatagcgtt tctttttgtt cattgatata ttagcgaccg
    16681 ttagtaccac aactaagctt acaatcatcc caatcatacc aatttggaaa tagtcattat
    16741 aagtatagct ttcagccgct ggattgaaac cagcataaat cgtttgatat tgtttgaaat
    16801 tgacgatatt agcaaagatt ccagccataa tcgagcccat aaatgagaac agtgcaaccg
    16861 gtactgaaat acctaatgac atcaagattg gcaacacaat aactgcaata gaaatcactg
    16921 gtccgattcc tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg
    16981 tgattcttgg cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc
    17041 cgctatctac tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt
    17101 tggcgtactc agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc
    17161 cttccatcgc cgggtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag
    17221 tagccatgat ggcaaagccg accatcaagt tgtagccttt tacacagtac cagaccatga
    17281 aaaagaatga gataactaac aaaataccaa taataatatc actcatgtta ttcctccgtc
    17341 cattttgttt ttctgataag caaactaata tatcagtcaa actcattcta acttatgtag
    17401 ttagcgcttt cattaaaggg tgaccataaa tgttcagttt tcaattaaaa aaccttaatt
    17461 atgctatttt agacttatac gttattgtcc tgtttcattt gatggaggat tgtaaataaa
    17521 aaaagaggaa agaacataac ttttcgttat cttctttcct ctttataatt gtttagctta
    17581 tttagccaaa gcttcttttg cttggtcagc caatgcagta aatgctgctg catcgttaac
    17641 agccaagtca gctaacattt tacggttgat gtcgatttca gcaactttca aaccgtgcat
    17701 taattttgag tagctcaagc cattcatacg agctgctgcg ttgatacgag caatccacaa
    17761 tttacggaag tcgcgtttct tttgacgacg atcgcgatat gcgtagttgt atgaattcat
    17821 tacttgttct tttgctgttt taaataaagt gtgttttgaa ccgtagtaac cttttgctaa
    17881 ttttagcact cttttacgac gtttacgagt tactgttcca cctttaacac gtgccatgta
    17941 taattcctcc taataaaaat aaatcttcag attctgttat ttgtacttct tatttcattt
    18001 ttgctagttg ttggcgaata cgtttgaagt cgccactaga aaccatgctt gctttacgca
    18061 attgacggcg ttgttttttt gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc
    18121 gtttcaatcc gcctttacca gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt
    18181 gttttggcat gactatattt cctcctcaaa atctttcgtt atccgactat cggctgtcca
    18241 acagccagtt agtcattcaa attacttact gtcgtttttc ggtgccagcg tcaagaacat
    18301 gctgcgtccg tccattttcg ctttttgttc cactgttgca atatccgcag tttcttcagc
    18361 taagcgattc aagacttttt gaccaatttc tttatgggta atggcacggc ctttgaaacg
    18421 gatagaagct ttcactttat cgcccttttc caagaactta cgtgcattac gaagttttgt
    18481 attgaagtca ttcaagtcga tcgtaggact taagcgaact tcttttacat tgatcacttt
    18541 ttgtttttta cgcgcttcgc ggtctttctt ttgttgttcg aaacggtatt taccgtagtc
    18601 catgattcgc gcaactggtg gtttcgcacc aggggcaact aacacaagat ccaagtttgc
    18661 ttgttctgca atattcaatg cttctgcttt tgtcttaact cctaattgct caccatctga
    18721 accgatcaat cgtaactctc gtgcacgaat gccgtcgtta accatcatat cctttgctat
    18781 ggtcattcac ctccaaatat tatgagagaa agaaaagctt accgaataaa cggcaataaa
    18841 aaaacgagtt ctcttacaga acccgcgcgt acttcaccta atagaaccag tgaaaactat
    18901 ctagcccagt gacattatca actaaggcga gaagcgggtg cttctgcttt tatttctcaa
    18961 ccatcatagt atagcaaaaa ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc
    19021 ttttctgaaa atgattagat tagcagactt tctagcagtg attcccgctt ctttgctata
    19081 ctaaagaaaa acgaggtgaa cccacatgaa aaaaatcgaa cctattccac cttctcttcc
    19141 tcttcttaca aagggagagt tttatttaga agatgaggag atcttctctg gtcttctgat
    19201 caacgggggt gaccaaagct atctcgatgc caaaaatatg gtattaagag agagtcatat
    19261 ccaaaaactt acgatgcaaa aaacagtttt acgtcatttt gaatgcagta atgtcatttt
    19321 tgaaaaatgt gatttttcca atctggagtg gattggtggc agtttccacc aagtcgtgtt
    19381 tcatcaatgt aaattaacag gtacgaattt tgccgaaagt tatcttcgag actgtacttt
    19441 cactgattgt attgcttcga tggcttcctt tagtagtaca aacttgaaag ctgtctcctt
    19501 cgatcattgt caattagaag acagcgaatt ttatgaggtc acttggaaaa accttttttt
    19561 gtctgacaat cagttgaccg gttcgaactg gtttcgaact tctctgaagg gattagactt
    19621 tacgactaat ttcttcacag caatcgcttt atctcaagat tctttggcgg gactaatcgt
    19681 caaccaagag caagcacttg tgatcgccca agcccttggt ttgcagatca aagactagat
    19741 caacgaatcc gccgccacag tgcaataagc atccaaatag ataagaccaa actgatgatc
    19801 aaggcgattc cccatccgaa aacggaatgg gtaaagggta actggagatt cattccgaag
    19861 aatcctgtca caatagttgg aacggtcaag atcagcgacc agaccgtcaa aaatttcatc
    19921 gtgtcattca agttgttatt cagcaaatta ttataagtgc cagagagctg gtctaaaatc
    19981 tgcgaagcta agttcgtcat ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac
    20041 tcttcttttt ccttttctga caacttgcga taaatagcca atgccttgat ttgttctaaa
    20101 agtactacat tttgttttgt tcctgtaact aagtaaacca aaccgatctc taaatctgaa
    20161 agttgcagca gattcttgtt cgttgttttt tctcttaatt tcagattaag ccgctgacgt
    20221 tcactattca cttcctcgat taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga
    20281 aacaataaac tgtatacact catgtctggc attcgttcca aaagcgatcg aattagtcga
    20341 atcacgtact cggtattcgg cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa
    20401 gcgataggac tagtttcata atgattatcg actttttgct ggtgcggaac atttgataca
    20461 agcagtaacg cttcatcaaa agaatcgtac tctacgcgcg cccgttcatt tttatctaga
    20521 gaataagtca gcatctcgtc actaatgcca tattcttctt ttaagttttt aacagctagc
    20581 acatcgtttt gctgtacatt gatccaaaaa ttacggtctt gattaaaata gtgtttttcc
    20641 cgcatactct atcctcctga catttgtttc cagtataaag aaaaaagtcg ctcaaataaa
    20701 ggtttaccct ttatttgagc aactgtaaat caaatggcta ctttggtttg gcgaatctct
    20761 gctaattttt cttcgatcat tttcaagact tcttcttttg cttgaggatc ttccacaaaa
    20821 ttatagcggt ctccatcgat ttggattttt ggactttctt tgtattcttc ataccattga
    20881 tcgtaacgtc tgttcaattc tttgtaataa tcgtatagac ttggatcaaa ggacaattgt
    20941 tcatacgaac gaccacgttt ttcgatacgc tccaacatcg tatcaaaaga tacacgaata
    21001 tgaactaata aatccggatg ttttttatgg gcagcatagg gaagttcttc catcatgttt
    21061 tccagcagtt catcatacac acgaacttct gtttctgttg ctcttcctag atctgcattt
    21121 agatggaaaa gcagtgaatc ttcataaatc gaacggtcca atacattatt gtcatctttc
    21181 attgcttgtt tgatgcttgc aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca
    21241 tactgttcag ggtttgcata aaataagggc aagacttcat tatcatcaat cgattcataa
    21301 aatgcctgac tatcaaagtg ttcagccatc atttctgtta aacttgattt tccggcacca
    21361 attgtacctg ctaaaacgat cacgctcatt tacctccagc tcacttcatc actaaatatt
    21421 gtgtttaact ccaacctttt taaatatacc cccaatatct agtagtgtca attgtatttt
    21481 tttctagtca aagaagaaaa aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta
    21541 gaaaacgtat tcttagtttt acttctgaga tggttttagt aaaataaagg aggaatgttt
    21601 tttgagaccc ttagtggaca aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt
    21661 attttttgct taaaaatcac ttttacgtga gaaggagtta gattatggca aactatggcc
    21721 aaggagaatc aagcggaaag atcatattga tgggcgagca cgcggttgtt tatggagaac
    21781 cagcgattgc ctttcctttc tatgcaacaa aagtcaccgc attccttgaa gagctggatg
    21841 caatggacga tcaactggtt tcttcctact attcaggaaa tttagccgaa gctcctcatg
    21901 cattaaaaaa tatcaaaaaa ttattcattc acttaaaaaa acagcatgac atccaaaaaa
    21961 acttgcaact gaccattgaa agcacgattc ctgctgaacg tggaatggga tcaagcgctg
    22021 cagtcgccac agcagtcact cgtgcttttt atgattactt agcatttcct ttgtctcgtg
    22081 aaatactatt agaaaatgtc cagctttcgg aaaaaatcgc ccatggtaat cctagtggaa
    22141 tcgatgcagc cgctactagc agcttgcagc cgatttattt tacaaaaggg catcctttcg
    22201 actacttttc tttgaacatc gatgcttttt tgattgtcgc tgatacagga atcaaaggac
    22261 aaacaagaga agccgtcaaa gatgtcgctc acctctttga aacccagcct catgaaactg
    22321 gacaaatgat ccaaaaatta ggatacttga cgaagcaagc aaaacaagca atcattgaaa
    22381 attcaccaga aacgttggca cagacaatgg atgaatcaca atcacttctg gaaaagctga
    22441 caatcagcaa tgattttctt aatctattga tccaaacagc aaaagatacc ggggcattgg
    22501 gtgccaaatt aactggcggt ggacgcggtg gctgtatgat tgcattagca caaacaaaaa
    22561 caaaagccca agaaatcagt caagcacttg aagatgcggg tgctgctgaa acttggatac
    22621 aaggattagg agtacatacc tatgtttaaa ggcaaagcac gcgcatatac caacattgct
    22681 ctaatcaaat attggggtaa gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt
    22741 tcgttaacat tggatgcatt ttatacagag acagaagtca tcttttctga tagctatatg
    22801 gtagatgaat tttatctaga tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt
    22861 cagtttcttg acctttttcg taaagaagcc ggcctctcac taaaagcttc agtaatcagt
    22921 cagaatttcg ttcctaccgc agctggatta gcctcctctg ccagcgggct agctgcttta
    22981 gcaggcgctt gcaatactgc tcttaagctt ggattagatg atctctctct ttcaagattt
    23041 gctcgacgcg ggtctggttc agcttgccga agtattttcg gtggtttcgt cgaatgggaa
    23101 aaaggccatg acgacttaag ttcttacgct aagccagtcc cttccgattc tttcgaagac
    23161 gatttagcaa tggttttcgt tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat
    23221 gggatgcgtc ggacagtcga aacatccagt ttttatcaag gctggttaga ttccgttgaa
    23281 ggggatctat atcaattgaa acaagcaatc aaaacaaaag atttccaact tctcggagaa
    23341 acgatggaaa gaaacggact aaaaatgcat ggaacgactt tagctgccca gcctccattc
    23401 acttactggt ctcctgacag cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa
    23461 ggcattccat gctactttac gatggatgct ggccctaatg tcaaagtttt agtggaaaac
    23521 agccatttat ctgaggtaca agaaacattt accaaattat ttagcaaaga acaagtaatt
    23581 actgcacatg ctggtccagg aattgcgatt attgaataaa gggagatttt tatgattgaa
    23641 gtatctgcac caggcaaact ctatattgcc ggagaatatg cagttgttga aacaggccat
    23701 ccagcagtta tcgctgcagt cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa
    23761 gtcggaagta tccaatctgc acaatatagt gggatgcctg tacgttggac aagacgcaac
    23821 ggagaattag ttttagatat tcgggaaaat ccttttcatt atatccttgc tgctattcgc
    23881 ttgactgaaa agtatgcaca agaaaaaaac attcttttat ccttttatga tctgaaagta
    23941 acaagtgagt tagacagttc aaatggccgg aaatatggat tgggttctag cggtgccgtg
    24001 accgttgcaa cggtcaaagc cttgaatgtt ttttacgcgt tgaatttatc tcagttggag
    24061 attttcaaga ttgcagcact agccaattta gcagttcaag ataatggttc ctgcggcgac
    24121 atcgctgcta gctgttatgg tggctggatc gctttctcaa ccttcgacca tccttggctc
    24181 caagaacaag aaactcagca ttctatcagt gagttacttg ccctggattg gccaggtcta
    24241 tccattgagc cattgattgc tcctgaagat ttacgtttat tgattggttg gacgggtagc
    24301 cctgcctcta cttctgattt ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg
    24361 gcttatcagc ttttcttaaa aaatagtaca gaatgtgtca acgaaatgat caaagggttt
    24421 aaagaaaata atgtaacgtt gatccaacag atgattcgaa aaaaccgaca attactgcat
    24481 gatttatctg caatcactgg ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat
    24541 ttagctgaac agtatgaagg agccgcaaaa tcttctggtg caggtggggg cgattgcgga
    24601 atcgtaattg ttgaccagaa atctggcatt cttcctttaa tgagtgcatg ggaaaaagca
    24661 gaaatcactc cactgccgtt acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc
    24721 gaaaagacga acatgtttca ctagctaaag cctttcatga taaacaaaaa aacgaatttg
    24781 acttcgtacg gatcatccat aaccctttgc cgcaaatagc agtttctgat gtcgatctaa
    24841 gtacacaggc agttgggttt acactaagca gcccatttta tatcaatgcg atgactggcg
    24901 gaagcgaaaa aacaaaaaaa atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt
    24961 tgatgattgc cactggttca gtcagcgctg ccttgaaagt cccttcttta gccgatactt
    25021 atacgattat gcgacaagaa tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga
    25081 cttctgtcga aagagccaaa gaagctatcc gattgtttca cgcagatgcc ttacagattc
    25141 atttgaatgc acctcaagag ttggttatgc ctgagggaga ccgtgatttt accaattgga
    25201 aagtacttat tcaagaaact caaacagcca ttgacgtccc cctcatcgta aaagaagtcg
    25261 gttttggcat gacaagagaa acgctcaatg atctagctgc tttaggagtt catacagttg
    25321 atatcagcgg tcgaagcgga acaagcttca cccagatcga aaacgcccgt cgctccaagc
    25381 gggaactgag ttatttagct gactgggggc aatcgacagt ctcttcattg cttgaagcaa
    25441 atgaagcaga cacttctatg gaaatcctag cctccggagg tatccgcaat gcctatgaca
    25501 tatttaaagc cctttgtctc ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc
    25561 atctgatgaa tcacggcgta gaagaaacta ttatactgat gaagcaatgg caagaagagc
    25621 ttcgtctgct ttatactatg gtcggcgcaa cgaataccgc ggccctacac caacaatctc
    25681 tgatcttttc cggaccagtg aaggattggt gcgaagcaag agggatcgac ttagtgaagt
    25741 atggaagacg cacagaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
    25801 taaacacgta caaacggcgg aagtaaaagc aactccttcg gaaataagct gaaattcaca
    25861 aaaatttgaa aagcaatttt cgtgaattcc ttcttatttc ttggagttaa atgcttctgt
    25921 cccagcctct tttttcgaat aaatgatgtt taatcagctg tccttcggta ttcgttcaat
    25981 ccgctgattt cacaacctct ccactaaaca gtgtactaaa gctcttttaa ggttgttttc
    26041 cgatataggc aagtatgcct ccgtccacat acaagacatg gccgttaacg aaatcagatg
    26101 cgctagaagc caagaagaca gatggtccag ccaaatcgat tggctcgccc cagcgtgctg
    26161 ctggcgtacg tccaacgata aattgatcga atggatgacg ttcaccgttt tcttgtgttt
    26221 cgcgtaatgg agcagtttgc ggtgtagcaa tataacctgg tccaattcca ttacattgga
    26281 tattgtattg accgtattca gaagcgatgt ttttcgtcag catttttaat ccgccttttg
    26341 ccgcagcata agcactgacc gtttcgcgtc ctaattcact catcatggag cagatattga
    26401 tgattttacc gccgcctttt tcgatcatat ccggaataac tgcttttgaa acgatgaatg
    26461 gggcatttaa atccacgtcg attacttggc ggaagtctgc cgcatccatt tcgatcatcg
    26521 gtgtccgttt gatgatccct gcattgttga ctagaatatc aactgaacct acatcttctt
    26581 tgatctgttg aaccatcgct tgaaccgctt cttcatcggt cacatcgcag acataacctt
    26641 ttgcatcgat gcctgcttct ttataatgcg ctataccttc atcaactgat tcttgattca
    26701 agttattaaa tacgattttt gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa
    26761 taccatagac agcaccggtg actaatgcta cttttccatc taaacgaaac atatccatac
    26821 taaattccat cattttctct cccttatgct tattttaatt gatccatttg caccatatcc
    26881 atgtctgtat aggtgatatt ttctccgcac attgcccaga tgaatgagta attgcttgta
    26941 cctacacctg aatggatcga ccagcttggc gaaataatgg cttgttcatt gtctacaact
    27001 aaatgttttg tttcatctgg tttgcccatc atgtggaaca cacgtgtatc ttcattggca
    27061 taatcaaagt agacataagc ttccatacga cgttcatgtg tatgacaagg catcgtattc
    27121 caagcgcttc ctggttctaa aatcgtataa cccatttgta attgacagct ttcgcataca
    27181 tttggatgga tgtattggta gattttacgt tcattcagtg tcaggccttc accagtctcc
    27241 attggtttta cttggtcgat actgattttt acatttggat atttatgatg agctggaaca
    27301 gaactaatat agaatttagc tggggtttca gcatcttgag aagaaaatac aacatgtcgt
    27361 gtttcttttc cgatatagta tccgtcttgt tttttcattt cttctttttg cccatcgatc
    27421 tcgatgaatc ctggtccacc gatattgatc acacctagtt cacgacgctc taagaagtaa
    27481 tcaacaccta gttctttgtc taatgtgatt tctaattctt tttcagtagg tgtcactcca
    27541 ccaaagatca ttcgatcgtt gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc
    27601 ttttctacta aaaattcttt tctcaattgt tccgttgaat aatgacggat atcttctgga
    27661 ctgtgtgtat aacgtgtttc catttcttgc attttcttct actccttcta tcgaatgatt
    27721 tttccagagc cgcatgctaa aaattgtgcg acttcttctt gactaaattg gttgcagtcc
    27781 ccatggatcg tatgcttcaa ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga
    27841 gacatattgg ataaaatccc atggagcacg ccacctgcaa aagcatctcc gccacctact
    27901 cgatccacga ttggggtgat tctatggact tgggattcca catattcatg gttcatccac
    27961 aatgtcccct tcaattcatt ttcacttgct gaatgaacgg tacgcttggt cgaataaaac
    28021 acctgaatat tcgggaactt ttcctgcatt ttttgataat aataaattag aggttgttct
    28081 tctgcttctg gcgctttctc gatacctaac aaataaagag catccatttt tccagctgag
    28141 cagatatcta ctaaaggtaa aagctgatgg attacttcgc tggcttcttg ctgtgtccat
    28201 aattttccac gatagttgat atcgaagctg atcaggcagc cagctttttt tgcagcttct
    28261 atcacctgtt tcgtcatttt ttgccatttt ttagacaaag ctggggtgat tcctgaaaca
    28321 tgaaatatat ctattccttg gaataactcc tctagcgacc attccaaatg atccatttca
    28381 gcaaaacttg atccggcacg atcatagatc actgaggcgg cgcgttcgcc actaccagtt
    28441 tccatataat aagtacctag tcttgatcca ccagataata cctgtttcgt atctactcca
    28501 taacattgca agtgcttttt gactgctttt cccaatgcgt tatcaggtac ttttgtagca
    28561 aaaatcgctt catgtccgta attagctaaa gaaattgcta cgtttgcttc ccctccgcca
    28621 taatgggcag cgaatcgttc acttgaatcg atacgggttc cctgctctgt ggacaatcgc
    28681 aacatgattt ctccgagtgt cactactttt cccataagca gctacctctt gatttctttc
    28741 atttttgctg catactgttg cgcgacttcc gttactttgt caaaatctcc tgtagctgcg
    28801 ggtgctaaaa ggttcccacc tactccgact gttacgacac cagcatcgaa ccactctttc
    28861 atattgtcca gactcacacc gccagttggc atgatattga cttgaggcat cggcgccttg
    28921 aaggcagaaa tgatacttgg tccataagcg cttccaggaa atagtttgac gatatctacc
    28981 ccgcttttca gcgctgtttt gatctcggta atagtcatac atcctggtaa gtaaggaatt
    29041 tgatacagat tgcacatctc tgccgtttct tgatcaaagg aagggctaac aatatactct
    29101 gcgccagcta agatagccaa gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc
    29161 actatttctg gttggtcttt gtagaatgac aaaagttctt tgattacttg atctgcttga
    29221 gggactgtaa aagtcaactc aataccagtt agaccacctt tgatgattgc gtggcaagca
    29281 ttcaaagctt cttctttgct ttttccgcgt accaccgcaa tcactccggc gtttttcaat
    29341 cgagataaaa tatcaatttt tttcatcttc tccacctatt tcctatttag aaattttatt
    29401 ttataacttg gaaatctata cccatattat acggcagaaa agaaaaattg caaacgcttt
    29461 tttcattatt tttgaattct ttgctcgatt tcgctttttg tttgcttgag ggcactgatt
    29521 atttgttctt ttgtctcatc tgttaaacga tatttcggca tacttacact aaatgcacca
    29581 atgatttgtt cgtttttcac gattgaggct cctatacaaa aaatatcacg ttcgatttct
    29641 tcatcgtcaa atgcgatgtg ggtttctttt accgttttga tttccttttt caaccgtaat
    29701 gcattggtga tcgtatattc tgtgtaaggg atcaacggat gcttttcaag ataatcagtg
    29761 tactgctctt cactgaattc tgctaatacg gctttgccca tcgcagaatt gtataatggt
    29821 cgggtgattc cgatttttga agacatgcgg atcgtttgat gttttggatc tagtttgttc
    29881 acgtacaaga tctcattgtt gtccaatacc cctaagtgga tagtctcatc gatcttttgc
    29941 tgcagctgtt ctaaaaacgg taaagtcaac tctaccaaat cgatttgttc aatgttttta
    30001 ttcgcgtagc gaataagctt tgcacctaaa cgatagtttt tctctgaatt tttatttacg
    30061 tagccgatca tcaacaaagt atctagtatt ttcaagactg tagaagaggt cattcccaca
    30121 ccctttgcga tcgtctgcaa agagacatct tggttttccg ctaaatagtc tagtatttta
    30181 gaggctttga tcaatacagt tccataaggc tttttatttt ccatataata ttctcctatt
    30241 tctttataag aaaaattata gcggaaatac catcaattta aaagagatat ccagatataa
    30301 actaccattt ctctttacag gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa
    30361 ctttgatata aagcaatgat ttcttctact gtcgtttctc ttggatttcc tggtgtgcat
    30421 acatctcgca aagcatcttc tgcaattgct ggaatatctg cttcttttac acctaattca
    30481 gaaatggttt ctgggattcc aaccgctttg ctcaaccgat cgatctcacc acaagccgct
    30541 tctcttacat cttctaaaga catttcagct gctcctttaa tccctagaac aagagcgatc
    30601 tcacggtact tttctccagt atattctttg ttatatttca tgacagttgg caaaagtgct
    30661 gcacacgcta cgccatgagg aatgttgtac catgcactta acgggtgagc catcccgtgt
    30721 accagtccta aaccgacatt tgagaagccc attcccgcaa tatactgtcc taatgccatt
    30781 ttttctcgcc cattttgatc gccatctaca gaagcttcca aagaatggcc gatgatttcg
    30841 attgctttta aatgcagcat atcagtcatt tcccaagcgc cttttgtgat aaacccttca
    30901 atagcatgcg tcatagcatc catacctgtt gatgcagcca attttttcgg cattcccatc
    30961 atcatatcgc tgtcgatgaa cgcgacgatt gggatatcat gaggatcaac acaaacaaat
    31021 tttcggtgat ttacttcatc tgtgatgacg tagttgatcg ttacttctgc tgctgttcca
    31081 gaagtcgttg gtatagctaa tataggtaaa catggatttt tcgtatcagc aacaccttct
    31141 aaactaatga cgtctgaaaa ctctggattc gtcaaaataa tcccaatggc ttttgccgta
    31201 tcgattggcg aacctccacc gattgcgatc aaacagtctg cctctgcctc tttaacaaaa
    31261 gccactccat cttttacatt ttggatcgtt ggattaggta cgatcccgtc gtataaagca
    31321 taagcgattg ctgctttatc tagaagttct gttacttttg ttgctatacc gtgctcgatc
    31381 aatcctttgt cagtaatgac agctgctttg ccgaatcctc gctgctgaaa ttcagggacg
    31441 atcgactcaa tcgctccttt tccaaaatac gatgtttcat ttaaaatcat tcgattactc
    31501 attttctatt cctcctaatt tgtattattt ttcacaataa aaacgaaaga accccaagca
    31561 accggttgcc cagttgcttg ttgctacagt tcaaagactg gcatcaattc ccaactgact
    31621 ggagaggcat ctggatttgt ctccatgata tcttccatgt acttccacca tttttgattg
    31681 atagatgtat ttgccatttt ttcccataac ttttcatctt cgatcactaa gtaaccaaat
    31741 aacaaacttg tactcggatc aagatagatc gaatagtcga tgaccccgtg ttcacgaagt
    31801 gctgtacgca tttctggcca aagttcatca tgtctttttt tgtattcttc ttgtttttct
    31861 gggtaaactt tcatgcaaaa agcttttttc accatgtcag acacctgctt tcgtatgaac
    31921 ttcctccaaa taacctgctt ttggagtgac accaaacgca tctgctaaat gccacaggtt
    31981 ttcgtccgtg attgtttgca agattggtcc ttgcgctttg acgtacgtat agacttctgc
    32041 tgccttttct gctgtctcga tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta
    32101 gattccatgt tgtggccata aaaccaatcg agtttctttc attttttcag ctgttgcttc
    32161 cccaatctca tttgttcccg gaaccatcca cggaatgatg gaaattcctt ctggaaaaac
    32221 aactaagcat tccgtacaca tttgccatag cgtacgacta aaggaacgtt cgtctaattc
    32281 atgcgtaaaa ctcatcgcta atagatgact agcatggcaa tgcataacga tccgattatc
    32341 tgggtctact tctaaacggg cgatatgact catgaaatga cttggcaact cgcttgtagg
    32401 aaggcattcg ttttccagcc cccacaaaag ttccagtgtt ttgccatctt cagcaactcg
    32461 aacgattcca agattctcgg caggttcgct cgctacattt ttaaaatatc ttcctgaacc
    32521 agttacaata aaatagcatc ctgctaaacg gctggcatca aaaatcatcg agatgcggcg
    32581 gatcactttt tcaggatcca agaacggtgt gatctcttct tcttttagta aataagaaat
    32641 attcccgcca tttctttcat cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat
    32701 ttctttcaca taaggcgctt gtaaaatatc tatttttttc aatatagcca cactcctatt
    32761 ttcttaattc caacacgtct ttttgatatt cctggacttc ttccagccaa tccaaaccaa
    32821 ctggaacttg atttttgtaa cagaaataat tccatacatc ggcatacggg aaatctttca
    32881 attcttccgt ataagccatt cttgaagtaa agtcaaaagc cagctccatt tctttcaatc
    32941 gctcaactgg ttccagcata gctttcagca gtgctttttg tgtatttctc gtaccgatga
    33001 cccaagcagc gatacggttg atcgttgcat caaagaaatc cagaccgata tttgtttttt
    33061 ccaacaattc gttacggact aattctttag cgatttcctg caattcatca tccatcaaaa
    33121 ccacatggtc tgaatcccaa cgcactggac gggaaacatg caacatgatt cctttgctga
    33181 ataaagctaa tgaagataat ttattggaaa tcacttctgt tggatgaaaa tgtccagcat
    33241 ctagacaaac aagtttgtca cgagtcaacc cgtaacccat ataaaattca tgggaaccga
    33301 ctgtataagc ttcagcgcct aaaccaaata gtttgctttc tactgcatct tgtacatacc
    33361 gttcatctaa ctcttctgta aaaatttcat cgagtgattc cattaaacgt tcccgtgggg
    33421 taaaacgatc aatcggatta tctttcatcc catcaggtac ccaaaagtta ttgatacttg
    33481 tttgcccagt ttctttccca aagtactccg cgattttgcg gcttcttttc ccgtgttcga
    33541 tccagtattc acgaacttct ttatctggat gagccaatgt aaagccgtct ttcatcattg
    33601 gatgcgaaaa gaaagtcgga ttgaagtcaa gaccgatttc ttcttttttt gcccactcta
    33661 cccatttttc aaaatgtttc ggctcaattt catttagatc gacagattct tctgtatcca
    33721 aatagatagc atgaaggttc aatttgtgtt tgcccggtat caacgaataa gccttctcca
    33781 gatcttgtct aagctcttca ggtgttcgtg cggctcctgg aaaattccct gtcgccatga
    33841 ttccaccggt caattcgcca tccggtgtca agaaaccttt gacatcatct ccttgccagc
    33901 aatgcataga aattttgatg tcctgtatct tttctagtac agcatctgta tctacaccga
    33961 tagttgcata tttttctttt gcttgttcgt aatttctttc aatcgtgttc atacgattac
    34021 tcctttgatt ttattcacgt accattccat cgtgttttgc ttattttcat ctgggtaaaa
    34081 agtttcacat ggaaacgact gtttgattac atttcttgct tgttctatat tcgaaaactg
    34141 acctgtactg agcatttgca tggcaacatt cccgattgct gtggcttcac taggtcctgc
    34201 ttctacaatg attccggcat aatctgctgt caattgattc agtagacgat tattcgatcc
    34261 gccccctacg atgtgcagct tttccaattt cgttccagtt aagtgttcta ctttgatcag
    34321 ctcttgtgcg taacaaagag caagattatc atacacacaa cgagcgatag ctcccgatga
    34381 gacagggact ggctgctctg tttcaaaaca gtattgctga atctcttcta tcatatttgg
    34441 gggattcaaa aatcgagggt cattgatttc catcacttgc tgcagcggtt tttcttttgc
    34501 agcaagctct gccaattccg cataactgta ctcgtactct tgcattcggg caacttcctg
    34561 gatcaaccac atccccatga tgtttttcaa aaaacgcacc gtgttatggg caccccattc
    34621 attcgtataa ttttcagaaa aagcattgcg agaaacattg gcgatctttg tttcaatccc
    34681 taaaagcgac catgtcccac tgctgatata accccaacgt tctccttgac ctggcgttcc
    34741 taatacagct gacgccgtat catgacttgc tactgtgata aaggtcacat ctggcaaatc
    34801 atattttgaa aattgttctt tttgcagatc tcctagtatc gttcccggct ctgtcaatgg
    34861 tggaaataat tccttcgaga caccgatttt ttcaagaagt tctatttccc attcttttgt
    34921 ccctgcattc agcagctgca tagtagatgc attagttttc tccgtcacca tgttaccagt
    34981 aaagacatac cctaaataat caggtatcag taacaattta tgtgcttttg ccaaattttc
    35041 tttttcttca gcaaacaatt ggaacaaggt attgaaaggc tggatctgaa tccctgtctg
    35101 ctcatacaac ttatctagag aatagatttc agaaaactgt tctactgctc cattggtacg
    35161 cttgtcccga taagcaatcg gatcatttat ccgatcccct tgttcatcaa tcagacagta
    35221 atcgactgcc catgtatcga tccccgcaaa aaggcgatca atccctgtct gctttaattt
    35281 ttcaaatcca atcaaaatct ctttgataag ataatcgatc ttccagcgat cgtgaccgtc
    35341 tttgttccga aagccgtttt tgaaccggtg tacttcttcc aagatcaaag aaccttttgc
    35401 atccctcgta cttttcatta gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt
    35461 tggattcatt tctccacctc ttttacttta ttgttgatta gcactttatt aataaagcgt
    35521 tatcatttat tgacaatgct atcataatac agaatcttat attttgcttc acactatttt
    35581 ggtatataat tgcattatct tgacaagttg ggagtgttta catggattac tgggaaaaag
    35641 ccaaccactc atggtcggaa gactcaaaaa gagtgatctt gacgcctaca caaagaagca
    35701 ggaatttatt tttttatatc caagaaatcg gttacttcaa agcaccaatc ccttactata
    35761 ctgaacgagc acatttacct tcttacttga taaagtttac tttgtctggc aaaggttcgc
    35821 ttacatataa tgataatcag caacagctaa aagctggaga cttatttttt attgactgcc
    35881 aagaatatca gcgttatcaa acgatcagcg aagaaccatg ggaaatggat tggatccatt
    35941 tgaatggtca gaacgtccaa tcattttatc aggagttcac aaaagacggc cataacacct
    36001 tccattcaac aggagatcca tcagaaaaca agatccacca tttgatcaca gaattgatcc
    36061 acatgcaaca aggcacgaat gcccgtaccg atttccagtc ctctgtagct atccacgagt
    36121 tgctgaatga actgattttg caaaagtatc agctagattt cgaagaagag gatattcccg
    36181 cctacgttta caaactgaag caatacttag acgaacattt caaagaaacg attacacttg
    36241 atcaattaga acaacaattc ttattgaata aatatcagct gagcaaagat ttttccaaat
    36301 ttatcggttc tccaccaatc gattatttga tcagcaaaaa actttcttac gctaaagacc
    36361 ttcttcggta tacagaattt acggttcgaa tgatttccga agaaataggg attgagaatt
    36421 ttgcttactt tagccgcctg ttcaaagcaa aaacaggact atcgccgaga gactttcgta
    36481 aattcagctg attcatataa tatgtacgtc attttttcaa aatattttga aatatataga
    36541 taaaaataaa ataaccgcag tttctgcttc tatgtaaaca acagcagcta ctgcggttat
    36601 tttttagtga tcattctcat tgttcatctt caaaatgttc ataatgaacc aactgtttct
    36661 gtgtatcacg tttcaaaaat tctttaggcg ttatgccgaa ctctctcttg aatgctttca
    36721 gaaaatgcgg ataagaagaa aaaccacttt tactatatac agcactagag ctgcggtatt
    36781 ctcgcaacaa gtgcttggca tagatcagct ttttcttcat tacatactga tgaaacgtaa
    36841 atccagtgta ctttttaaat tctcgtgtga tatggtattt gctgaaaaaa aactgctttt
    36901 ccatttcctc taaagaaagc ggatgagata aattttttga gatatattgt aacatttgtt
    36961 ccacacgctc attaggaact acagcattct gttcttgcgc actctcttct ttttgtacaa
    37021 cttgattgag aaaaatcaag tattctacca gagcttgttc atattttatg tcttcaccgt
    37081 aaattttttt atcaggagga tggtcgataa atgataattt ttgcttcaat acttcctgtt
    37141 ctatttgcaa tacacgactc ctcttctctc ccattggttg aaaacaagcc gatagattag
    37201 accatggcgt ggaacgactt tctagaaaag aaggggtgac aaagatatag acacgctcga
    37261 atacatctga agattggcgg acgatacgat gcaaatcatt ggaatggatg agcagcacac
    37321 ttcctgcttt gatcgtaaat tgtcgtccgt tcaaataaaa agtcgcttct ccctttaatg
    37381 tagcatggat ctcatagaaa tcatgatgat gatagatcgt tttattttgg ctgacgacat
    37441 ctttgacatg atagatttca tactgatcag aaaccatcgt attgacttgt tcaccgctga
    37501 ttttcttttg gagcatgtgc cgcacctcct tttgttcact tatgcacaat tttattatac
    37561 cacgtttttt gtaatcaaaa agagctgctt ttatatactc ttataaaaaa gaagagacgt
    37621 ggcatcttct agccccgccc cctccattct aaatattagt taaccaacgt cttctttaca
    37681 ttattcaaga agacttcttc cataacgaat cccatcatct tttgtttctt caagaaccac
    37741 atacaggtac ggacgattct tcatgatgat ttctttgatt tttgtgtact cgatcacacc
    37801 gtctcccaaa tttaccggca cgattttttc ctgttcgaca cgaaaatctt tcaaatgaac
    37861 cgcacatatc ttttcaccaa aacgttcaaa agcttcttct accaattgga cttgttcttg
    37921 ataatcacta cttgtgatca aattggtcgg atctaaaatg attcctagat agtccgaatc
    37981 tactgcttgg atcagctgct ctacccttgc taaagaatac accggatgat tcaaacctgg
    38041 ttctatcccc accatcattt ggtatttttc tccagctttt accaagcgac ggataacgga
    38101 cacagcttca gcaaatgctt catctgtgaa attttcttcc gtatattgga tttccggcaa
    38161 aacacatcca gtttccgaag caaccatcga cgcaccaaag gaagaagcat agcgaagata
    38221 ctgctcgaac tttgtcagca actcttgtcg tatcgttaaa tctggatgaa tcatattgat
    38281 atagcaactt aaaattccta cggtcacttc ttgcttttct aaaactcgtt tgacataatt
    38341 tcccatccca gaattgatct catttgcgcc agaaggtatt tcaggaaacg aaagacctaa
    38401 ggcgagctgc agtgtatgaa tcccttgttc ttttgttttt tctgcaagat cttctatcgt
    38461 ttggacttcg gaaagatcat gtcctctaat tgtaatgttc aacataacta ttctcctaac
    38521 tcagcatatt gggtagccac atgacgattt gcgggaagta agtaatcaaa aacaagacga
    38581 ctaagataac cccataaaaa ggaagaagcg gtttcagcat tgcctctgct tttacgcctc
    38641 ctacacttgc accgacatat agtcctgtac ctactggcgg cgtgatggag cctacacaca
    38701 gattcatgat tgaaaacaac ccgtaatgta caggatcaac ccctacactt gtgacgattg
    38761 gtaaaaatat tggtgtaaaa atcaagatag caggcgccac atccataaac atacccacca
    38821 gtaataacac aagattgact aataacagaa taataaattt attatccgtt acgcccataa
    38881 tcaacgaact aattgcctga ggaataccgg taaacgccat ggcgaaagac atcatggaag
    38941 aagtagccaa taagaacatg atcgtccctg acattgctac tgattgaaac aacatattag
    39001 gtatatcttt tagttttacc gttttatagt aaaacatagc aatcaacagg gagtaaatga
    39061 cagctatcgc tgaagcttct actgctgtaa atatccccgt caagatacca ccgataatga
    39121 tgacaatcaa tagaaggcta ggaatcgcct cttttattat ttttccagca tgagccatct
    39181 gtcctttttc gacaacaggg tatttattct ttttagcaac gatgaatgct actagcatga
    39241 ttcctagcgc ccacaaagcg cctacgagat aacctcccat aaacaaagca gagatcgaag
    39301 caccaccagc aacaagtgaa tacatgataa aagcagtact tggaggaatg accatacctg
    39361 tcggagctga cgcgatattg accgctgtcg caaacttccg gtcatatcct tcttccactt
    39421 gctgagggat caacactccg ccaatcgcgg tagaagcggc aatggccgag ctagagatgg
    39481 aaccaaataa agcattccct aacacattcg tatgagccaa agcccccggc acgcgcccga
    39541 cgaacaacat cgcaaaatcc actagtttct tagctatccc tccattattc atgataatgc
    39601 ccgagagaac gaaaaacgga atagcgatca gcgaaaaact attgatactt cccaccattt
    39661 tttgagctga actaaaagca gaaacatcaa acggtataac taacaataaa gtcatcatcg
    39721 atgagaatgc aatacttata gcaatcggca tccctaatac aagcaacaca acaaatacaa
    39781 aaaataacac taatccggct tctattgcca ttttattcct ccatttttat attttttctt
    39841 gaattgctgg cttgttgctt aatgtatcga ttagattgat caggctatag ataatgatga
    39901 gtacccccga tacaggtact gacagataaa catatcccat aggaatactt aaagaaggcg
    39961 agatctgccc cattgtcaat gtgacagcat tccatccgcc atacaccatg atcatcgcag
    40021 caaataccag aaataaactt tgaacaacaa cttctaaaat tttacttttt ttgccagtta
    40081 atttttcact taaaaccgtt acggctaaat gtgctttttg tcccacaaca tacgcggaag
    40141 acaacatggc aaaccagaca agaccgaatc gaacaagctc ttccgtgacc gtatttggat
    40201 ttcctaaaac cgttctggaa aatacttgcc ataaaacaac agcgaccatc ccaacaagca
    40261 aaacagctcc caggatctct aataccttgt ttaaaatcat tttcatcaca gctacgctcc
    40321 ttgctcttgg atcttttgat aaaggtcttt gtaggtagga tcgtttttga atatatcatg
    40381 aagtggggca actgcctctc gaaacggtgt attatctaca tcattgaatt ggactccatg
    40441 ttcttcttgt gctaaagctt tttcttcttc aattgctttt gcaaaagctt caatttcaaa
    40501 aaccgttgat tctttcgctg cttcttcaat ggctttttcc tgctcatcag ttagctgttc
    40561 tttgatggca tcattcatga tgatgatatc tggcacacgt gtatgctcat cataggaata
    40621 gtatttagcc acaccaccat gtccagctgt atacaagaca aattcattgt tttctgatcc
    40681 gttgatcaaa ttggattgaa gagacgtgta cacttcatca cttcccatag gtacgggcga
    40741 accgcctaaa agctctacca ttttgatagc ggtttcgctt tgcatcacac ggatcttctt
    40801 tcccttcaaa tcttccggcg tatggatcgg accgtctacc atataaaagc tgcgctgacc
    40861 agaatcataa taggtcaatc cggtaaatcc taaatttttg gtcgattggt agattggctc
    40921 cattatttct tcattatcca tcaccttaaa gaaatgttct tcatcattga atagataagg
    40981 aactgaaaaa atcgaataat ctttagagaa actttccaac gctgaggcac ttacttttgt
    41041 gaaatctact gctcctgact gtgtcaattc gatcagttca cgttctccac ccagttgtcc
    41101 atctgggaaa tactggatct gtacggaacc atctgttttt tcttttacta aacgcccaaa
    41161 ttcattcatt gcatcaatta ctggctggct gttgcttgca taagcaaagc gcaaggtaac
    41221 tatctcattt acatctgcat ctgcgacagt tgccacacta gaattatttc cgcagccgct
    41281 tagacctagc agacctaggc taagcgaact tgctgcaaac agcagtgatg tttttttcat
    41341 atgggtttcc tttcttttaa caataagaga cgagcaattc tgtcaaacaa agcaccgtta
    41401 acgactgtcc ataaggcatt gctgtgattc cgatattccg ataaaaatct aaactgtccc
    41461 ccattcctgt cccgattgaa acgttttgaa cttctccttt ttcatcgatt tgttcaagca
    41521 atccttggat agcacgataa gcgacttctt tatattcttg tggtaaataa cgtttatgga
    41581 ccgctttcaa aataccgtaa gcaaatcctg cagttgcaga agattctacg taagaagaag
    41641 gatcatccaa caacgtatgc cataaaccgc tttcagactg ataagttttt aatgcacgca
    41701 cttgtgcttc aagtgtttcg atcaataatt tgcgcaatgc atcttctttc gataattcta
    41761 agatttcaat gatctctgga atagcaatcg tcaaccagca gtttccacga gcccaaaatg
    41821 cttcggcata gttatgattt ccttcaaacg tccagccgtg ataccacaat cctgttcgtt
    41881 tatccatcaa gtatttgata tggatcataa actggtaacg agcttcttct aaatattctg
    41941 ggcgattcaa cagtttcccg atttttgcca aaggaagcac tgtcatcatc aatgtatcgt
    42001 cccacaactg attcttattt tccggtccat atgtggcatg ctgtaacccg tcttcatttg
    42061 tccgaggcat gtcattcatg acccattctg cccattgttc taaataaggc aaatagcgtg
    42121 aatctttcgt gtcttcatac aagtaagcca tagtcaatag cggtgccatt gtattgacgt
    42181 ttttgggcgg agcgccttct tccatacgat cctcaaacca ttcattgacc acttgataag
    42241 cgcgaggatt ttttgtcatc tggtaatttt tataaatacc atatagaccg acaccttgtg
    42301 gccaattcca cactgtccag cttttgtcat ctactttcag tccatcaaag tccaacaaaa
    42361 attcaccatt atcgtctttg atcgttgtca ggttttcaac gagcaggtca attttttctt
    42421 tcactaattc tttaggtaca aaaatctctt ctttaataat catctttacg ccaccttttt
    42481 aaacgctttc attttatatc cttaatatag cagtaatacc gtccactata aatatcgctt
    42541 tttgaacaaa aactgtctaa aattgaccta aatgttaaat aacaaaagta actttcagtt
    42601 acttggaaat taaaaaaaga acttggagaa acaacgtttt ccaagttctt tttcatctat
    42661 tttcttatct tttttctacg ctttcttttg cttatccgtt gtaagcatca atgatgatct
    42721 gtttcaattc agagatcaat ggttctttcg gattagctgt cgtacattgg tcttcgtaag
    42781 ctaattcagc catacggtct acggttgtat ctagtgtttc ttgtgtgact ccttgagctt
    42841 ttagactcat gttgatgccg acagattttc ctaattcata aacagccgtt gctaaagctt
    42901 cgactaattc ctctgttgtt tcgcctttca gacctaagaa ttttgcgata tctgcgtaat
    42961 ctgtatctgc acggaaatag tcatatttag ggaacatcgc atgtttttgt ggatcttttg
    43021 cattatagcg gataatatgc ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt
    43081 attccccgcc gattttatgt gcgactgagt gacaaatacc taggaatgcg ttagcaaacg
    43141 ccataccagc cattgtagaa gcgttgtgca ttttttcacg tgattccata tcaggtgttt
    43201 ttactgattt ttctaaatag tcaaagacta acttgattgc ttgtaggctc aatccacgtg
    43261 tataatctga agccatgact gaaacatagg attcgattgc atgtgttaac acatccatcc
    43321 cggtatctgc tgtgacagat gctggaactg acatgacgaa ttgaggatcg acgattgcga
    43381 catctggcgt caatgcataa tctgctaacg gatatttcac atgtgtttca ctgtcagtga
    43441 tgaccgcaaa tggcgttact tcagaaccag tccctgatgt tgtcgggata catacgaatt
    43501 gtgttttttc tggtttgtcg atcttatatg tccgtttacg gatatccaag aatttttgtt
    43561 ttgcaccaaa gaaagatgtg tctgggtgtt catagaacat ccacattccc ttagctgcgt
    43621 ccattgcaga accgcctcca agagcgatta ctgtatctgg cttgaagtct gccatcagtt
    43681 tcgttcctgc gtatacggta tttgtcgatg ggttcggttc aacatctgag aagacttcga
    43741 tcttcacatc atttttacgt cgtgccaata cttcacgaac tgtatcgcag taaccgaatt
    43801 ggaccatacc agggtcacaa acgatcatga cacgttcaac gttttccatt ttctctaagt
    43861 acaatagaga gttcttttca aagaagattt ttggcggtaa tttgaaccat tgcatgttat
    43921 ttctccgttt cgctacagtt ttgacattga ttaagtttac agcagagacg ttacgagaaa
    43981 cagagttctt accataagaa ccgcatccca aagtcaatga cggaatcatt tcgttataga
    44041 tatcaccgat gccgccttct gcagatggtg tattcactaa aatacgacaa gctttcattc
    44101 gaagaccgaa tttgacatgc aactcttcgt tttctgaatg gataactgct gtatgtccta
    44161 atcctccaag attcaacata ccttcacaca aatcaaacgc atggtccgtg ttgttagctt
    44221 tgatcatggc aagaactgga gaaagttttt ctcttgatag cggataatct gggcctacgc
    44281 cttccaattc agcaatcagc attttcgttc cttttggtac actgattcct gccaattttg
    44341 cgatttccat cgcagaatgt ccaacgattg atggattgac cgcatatttt ccttcgttca
    44401 tcactgcatc ttctaatttt tgcaattcat ctggttttac gatataaact tggtgtgctt
    44461 ggaattctgc ttttactgct gcatagattt ctttatccac gatcacagct tgttcagaag
    44521 cacaaatcat tccattatca aatgttttcg aaacgatcaa atcgttcact gcacgtttaa
    44581 tttttgctgt tttttcaata taagctggta cgttacctgg acctacacct aaagctggtt
    44641 ttcctgtaga gtaagcagac ttgaccattc cagctccacc tgtcgctaag acgatcgcga
    44701 tacctggatg gttcattagc atagaagtcg cttcgatcga aggatgttcg atccattgga
    44761 tacaattttc aggagctccg gcagcgatag cagcatcacg taccactcgt gctgcttctg
    44821 cagaagattt ttgggcactt ggatggaaag caaaaacgat tggatttctt gttttcaacg
    44881 cgatcattga tttgaaaatc gttgtggaag tcggattcgt tgttggagtt actccgcaaa
    44941 ccacaccgac tggttcagca atttcaatca accctttttg gacatcttca ttgatcacac
    45001 caactgtttt atcatgtttg atattatgcc aaatatattc ggacgcatac atatttttga
    45061 ttgctttatc ttcgtaaaca ccgcggcctg tttcctctac agccatcttc gctaatggca
    45121 tatgttgatc caaagctgcc attgccattt catgcacgat atggtctact ttttcttgat
    45181 caaagctttc catttctttc aatgctgcat ttgctttttc tgccaaatca tcaatcattg
    45241 cttgtacgtc aattgtcttc gttttttctt tttctattac tttagccatc attatcctcc
    45301 taatgggtca tggttatttg ttaaataatt cacatgtata tcatacaata tttatttttc
    45361 tatgtaaaca cttttttgtg atttttttca cagttatttt taaatataaa attgctttat
    45421 ttacaggtgt ttgtatagaa aaatatataa agttatttat tattatttat atgacacacc
    45481 cgattattca atattgagca atatatccca tgctcttatc tctttctttt ttgaataaaa
    45541 aatgaggtag gacatctttc tgccctacct cgtctttaaa taagctttga tacttatttt
    45601 tcttccttct cttctgtctc ttccagcgtt tctgttgttt cagtttttac ttcaactgtc
    45661 tcagctggtt cttctgttgt ttcaacagta gtcgttgcat catttgttac gacagtccct
    45721 ggtttgaccg ttttgattgc tgcacgatcg tactcaagga agatcccttc gcagtctaaa
    45781 acaactgtcc gtttgtcatt gtcgatttct gaaacaactc catgtaatcc gccgatcgtt
    45841 acgactgaat cacctggttt catgctatct aacaagtttt gacgttcttg ctgttgtttc
    45901 ttttgcgaac gtgacataaa aaaccacatt gctaatagcg cgataaacat cacaagcatt
    45961 ggtaatgttc ccattttttt cacctcacta attctgtatc tacaaaaaac actttagcag
    46021 aaaaatgctg ataacactag ttttttactt aatttttaaa agattttcct ttagaagctt
    46081 tttgcgtttt ctttattgaa cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa
    46141 ttatcatcca tgattgcttg acgtacttgt ttcattagat tcaacaagaa gtataaattg
    46201 tggtaagaag tcaaacgaat cccaaatgtt tcatcacatt tgatcaaatg gcggatatag
    46261 gcacgtgtat agtttttaca tgtataacaa tcacatttct catcaattgg acggaaatca
    46321 tgggcatatt tcgcattttt cactactaga cggccttgag aagtcataca tgtgccattt
    46381 cgagcgattc gagtaggcaa gacacagtca aacatgtcta ctccacggat aacaccatcg
    46441 atcaatgaat cagcagcacc gacacccatc aagtaacgtg gtttgttttc ggggatcaac
    46501 ggtgtagtga attctaatac acgattcatt tctgatttag gttccccgac agataacccg
    46561 ccgattgagt aaccagggaa atccatcccg actagatctt tcgcgctttg acgacgaaga
    46621 tcttcaaacc ctgctccttg gatgattcca aataatcctt gacggtctgg attagcatga
    46681 gctttcaatc ctcgttctgc ccatcgagac gtacgttcta ctgatttttt cacgtagtcg
    46741 taactttcat cgaatggtgg acattcgtca aaactcatca tgatatcaga gcccaattta
    46801 ttttggatgt tgattgcttt ttctggtgat aaaaacatac gcgaaccgtt caagtgattt
    46861 ttgaaatgca cgccttcttc ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag
    46921 ccgcctgaat ctgttaaaat cggttgatcc cagttcataa atttatgcag tccgccagct
    46981 tcttctacta agtcttcacc agggcgtagc cataaatgat atgtattact taaaataacg
    47041 ccagcaccca tttctttcaa ctcttctggg gacattgttt ttactgtagc taatgttcct
    47101 actggcatga acattggtgt ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc
    47161 gcgccagtat gtttttcttt cttgattaaa cgatagcgaa tggcaggttg cgtcattcat
    47221 ttccctactt tctatataaa atatcttgac aactcccacc atcataatcg tttcaagtgc
    47281 tttttgcaag ttccctcggc ttccttctac tttatttaat attctttttt ttatcctttt
    47341 tttatgaata acaaaaaaaa gaaaaacatt atgacagaaa tattttttga tacatggtat
    47401 attggtgtta ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga attaaaacgc
    47461 caagcaaaag agagtctaaa aggacgttgg ggacaagctg ttttactaaa tctaatccct
    47521 acattgattg gtattgctac tattcttctt attatgattc ctattatctt gtttgccacc
    47581 accatatatg atggatcagc gagcgtctca gaagcgacaa cttcaggagg cggtagttca
    47641 ggaggcggtg gcggaattat ttccagcatc attagcgctt tgttcatgag cggtatttct
    47701 tggacttatc ttgatatttt acgtggacaa aaagatcaga tcgaaccatt caaagatgca
    47761 tttcgtggat tcaaaggttt gtttatcggc ggagtgatca gcttagctgt gctgatctcg
    47821 atctttacaa cattatgggg tttgctatta gttattcctg gaatcattaa aagctattct
    47881 tattctcaaa gctattttat ttactacgat atcgtccaag aaacaggaca aacacctaaa
    47941 gtactggata cgatcactgc aagtcgaaaa ttgatggatg gttataaagg acaacttttc
    48001 tggttagatc tttcatttat cggctggcat atcttagcat tagcaactgc tggtattggt
    48061 tatctatggc tcagcccata catttctgca acaaaagcag cattttatga acaattgcca
    48121 aaagatattt aaaagagagt gtgacttgtg accgaggggg tggcaggaga acaccgttta
    48181 tcgagaggca gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg acaacattca
    48241 tgtcacacct cctttttgta tagttccagt aaaaagaaaa tcaacttaga aacagttaat
    48301 cattcctctt cttctgcttc cggtaatcga tggaacttga tcagttcatt caccgcgatt
    48361 ggtacgatag ataaaatccc taccaagaac cagtgattca aactgatgtg tactaggccg
    48421 aatagttctt gcgtaaatgg cagcaaagca acagccgttg taatagctaa tgccagtaac
    48481 gccatttcta ccaaagcttt attggtcgct aaattgactt tgaagatcga ttggctgctg
    48541 cgtacattga acacatgcag aatcgatgaa taagctaaga tcaagaatgc aacagtttga
    48601 ccgacatgct gacttgcgtc tacccatggg ctgatagtat cgacataact acctaaataa
    48661 taaccaaaca aggttacgat ggtgaagacc cctgcattta tggcaatttt ttgcaataaa
    48721 cctcgagcaa agatcccttc atttttcgga atgggtggct gatccatgat gccgtgttcc
    48781 gctggctctc ttcctaaagc aaatcctgga agtccatcag caactacatt gacaaacagt
    48841 agttgcacag ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa aacaataaag
    48901 atctccgaaa tattacagct tagcaaaaag ttgatagctt tacgaatatt ctgataaacc
    48961 gaacgtcctt gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata
    49021 tctgcggcac tttgcgccac atctgttcct gtgatcccca tggcacaccc aacgttactt
    49081 gctttcaacg ctggtgcatc attcacacca tccccagtca tcgctacgac tgcacccgta
    49141 cgttgccaag atttgacgat ccggatcttg tcttctggcg ttacccgagc atagacagag
    49201 agagctttga cacgcgcatc taattcttcg tctgataatt gatgcagttc tgatcctgaa
    49261 agtgcttctg acggatcatt caagatacct aattctttcg ctatggcact ggcagtcacc
    49321 acatgatctc cagtgatcat gactgttttg atgccagcct ttttcgctcg cttgattgct
    49381 cctttacttt ctggacgtgg tggatcgatc attccgatca gaccaatcaa acgtaagtct
    49441 ttttccagtg cttcagaaga aatatcttgc ggttcttcgt cataagtagc ataaccaaca
    49501 gcaatcactc tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc ttgttctaca
    49561 tcgcctgaac taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat tgagatgtat
    49621 ttttcgccca tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg
    49681 tttactcgtg gatatttttc ttctaattct gcctttgtat gataattctc ttcaacagcc
    49741 cttacgatgg ctacttctgt tggattacct ttgataacaa ggtcgtcatc ttcttgctcg
    49801 accgtcacat ctgtacatag tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt
    49861 gaatcttcga tattcgttac ttcatcttct ttgctccata cccggcggac gcgcatttca
    49921 ttttgtgtca gcgttcctgt tttgtctgag cagataacat tagcggttcc tagcgtttca
    49981 actgctggca actgtcggat gatcgcatgt ttcctcgcca tcttctgcac accgtatgca
    50041 agtgtcaacg taacaataac agtcaaggtt tccggtacag ctgcaacagc caaggaaatc
    50101 gaagtcatga acatctcaag gagtggttct ccttgcagtt caccaatcac aaatactaaa
    50161 gccgcagccg ctaaagcgat catactgatt cgtttgccta gctgattcaa tcggacctgt
    50221 aaaggtgttt tttgcatagt attttcgttc aaaagatccg cgatcttacc catttctgta
    50281 gccatgccaa tggcagtgac gatcgctttc ccacgacctg cagcaaccgt acatccttta
    50341 aataccatat taagttggtc acctaatgac tcatcttctt cagcaataaa tgctgcatct
    50401 ttttcaactg cttcactttc tccagtcaac gccgattctt ctacacgtaa ttgtgatgct
    50461 tcgatcaaac gggcatcggc agaaatagca tcaccggact ctaaaacaag aatatcaccc
    50521 actaccagtt gctctgactc aacagtggtc actttgccgt ctcgtatgac agttgccgtt
    50581 tgtttattca tatcttgcaa ggattccagt gctttttctg cgtttccttc ttggacaatc
    50641 gccaataccg agttgatcac aacgatagcg atgatcagca gtccttcaaa taaatcccca
    50701 tgttctgtag caaatgcggt ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt
    50761 gtaaaatcgg acaagcttcg aaagaatttt ttgatcatcg attctttcgg cgcctcatcg
    50821 aatttattca atccctgctt ttttcgccgt tcatccactt cttgtgaaga caacccagta
    50881 tctggatctg cttctgttac cttctttact tcttctattg tctgcttgta ttcttccatc
    50941 ctattcctcc tttaaaatct tcgtacaaaa aaaggtactt attttactat aacatccttt
    51001 ttttatttac ttcaaataaa gggcttatcg attatttgtt aagaaagcgt aaaatttaaa
    51061 gtgcttatat ttgcaaacgc taccaaagga tgctattata tagatgtaag gaaggaggat
    51121 accatcaaat agacagaaga atttctcgat agaacagtgt aagcaaacaa agttgttaga
    51181 agtacaggtt tcacccagac atgcgtgtag agtacaaacc tataaatagt gaatgcgtat
    51241 ggatgtttgg atcacgaagt atccgaaaca agaagtaatg cgatacgtaa caattgaata
    51301 acgtaaacgt ctgaaaattg aataggaaca agagatgttt caaatgttga agatgtaacg
    51361 aaaacaaaat ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat gtatgatgga
    51421 aagttacaat catttctgtt tatttgaatc cttccgaggt acaagaaagc cgagattatc
    51481 gagagctcgg ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact
    51541 agcggctttt cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag
    51601 cgctcttcaa tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta
    51661 actaacatga ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact
    51721 ttgaattcat agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct
    51781 ccattaaatt tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg
    51841 attttcccgc catttttacg tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg
    51901 tagaattcac tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta
    51961 cctaacccta catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca
    52021 agtaattctt tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc
    52081 gcatagacag tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat
    52141 ggcatttctc ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg
    52201 atccgtccgc cgtgttccaa ttcttctttc actactgctt gcaatctgcc atcaccaaaa
    52261 gagattcttg tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct
    52321 ccttgtgtat tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca
    52381 tataaacgag caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac
    52441 tcttcaatga tttcatggaa atgtttatct tccatctctc cagttttccg atccaaaaca
    52501 agtaaacgag aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc ttccggcaag
    52561 tcaaaatcaa aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat
    52621 cattctatca tgtttttcgt tctgttggca aaatcagcac tttttcactt ctattttttg
    52681 ttttcctaaa taaaaaaact accctatttg tattttttca ttgttttagg catactagta
    52741 atagatagtt cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg
    52801 tgtcaaaaat ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa
    52861 agggtgagtt catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga
    52921 tgatcaaccg aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc
    52981 aagaaattga tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat
    53041 tgatgcctca catgacgatc cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca
    53101 gtcaagaaga acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg
    53161 agatgttaga tcgctaccca catgaattat ctggtggtca gcagcagcgt attggagtcg
    53221 ttcgtgccct tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg
    53281 atccaatcac tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa
    53341 aaacgatcgt atttgtaacc catgacatgg atgaagcgat taaactagca agccgtatcg
    53401 ccatcatgag tgaaggccgt gtgatccaat ttgatacacc agaaaatatt ttacgtcatc
    53461 ctgcaaacga tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa
    53521 atgcgacaag agtcggcgaa gttatgctaa atacagcaat cacgattaca ccggaaaaat
    53581 cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg
    53641 ataattcgaa tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aaacgaggaa
    53701 aagtatccag tgtcggcgac attttgaata aacatgtttt ttacgtaaaa gaatccgcct
    53761 atcttcgtga taccttgcaa cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg
    53821 atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg
    53881 atgtgatttg gggagaagat cccgcaacta ctgatgttgc agagtcagct actcctgaag
    53941 aatctaagga ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct
    54001 caaaaggttt tgaacatctt tatatttcag gacttgcttt gatcattggt atcatcattg
    54061 ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct
    54121 taaccagtgc cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat
    54181 ttggcgtagg taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt
    54241 tacgtaatac gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag
    54301 gcatggggat gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa
    54361 cgatcatggc tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct
    54421 cttatatcgg cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac
    54481 cagatctcat tttggcaggt acgatccctg taacaatttt agcactattg gctgattggc
    54541 ttctaggtct cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat
    54601 gaaacgtata ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc
    54661 cttccctggt cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac
    54721 ttcagaagca cagatcttag caagtctagt tgccggaatg gttgaacatt atacagataa
    54781 aaataccgca atcatcaaca atctggctac tacaactatc aaccatcaag cgatgatgaa
    54841 cggagatgct tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa
    54901 ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa
    54961 aagatacggt caaacgtggt tcccgtccta tagttttgaa aatacgtatg tctttcttgt
    55021 tcgaaaagac accgcacaaa aataccatct atctaaagtc agcgatctga aaaatgtagc
    55081 ggatgaatta gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga
    55141 cggcttccaa gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact
    55201 tgtttatgac gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg
    55261 acggatcgcc agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata
    55321 tgatgctgct ccagttgtag acaacaagat attagctgat acgcctcatt taaaagaagc
    55381 cttaaataag ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc
    55441 agataataat ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta
    55501 ttttgaaggg aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat
    55561 ttccaggaaa atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc
    55621 tatggtgtcc tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga
    55681 cgtaaattag caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta
    55741 gccatgatct ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact
    55801 gtatttttgt attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta
    55861 gacaaaaaca tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc
    55921 atggtagagt taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt
    55981 gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc
    56041 attatccgag gaaccaatgc aacagagggc acatcgatta ttttagcagg tgcattgcca
    56101 actgcattaa tggcgatcat cacggactgg cttttaggta ttttggaaag acggctagat
    56161 ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt
    56221 gttggtcgca aaggcttttt tagttctgtc atttttaagg caataatcca ctctgattct
    56281 tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg tatactccaa
    56341 cttcatactg ccaacgggtt catatctgca tccaccgact aagttttttt cttatccgct
    56401 aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc
    56461 taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc
    56521 tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct gcatcatcaa
    56581 aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat
    56641 cctcatcctt ggctaaaaac atatctagcc attccagctt ctcgccatgg gaggtttgcg
    56701 gttcctaaaa ccggtacacg attcttatgg actgcatccg tcgcaactag acttggcgat
    56761 acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata
    56821 agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct
    56881 attcatggcc gaaagcgcca cgactttaac ttcttccgtt tgagtgacat tcatgttcgg
    56941 caatgtcgct ttggataccc gctctgctaa aggaacgaca ctggtattgt atttcgtatc
    57001 tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg
    57061 ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc
    57121 aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa
    57181 cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac
    57241 ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac
    57301 aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct
    57361 cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca
    57421 taatgtgtcc aggcagaata aatttctaca actttatttt cctcatcaac tttgtaaacc
    57481 actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttt
    57541 tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat
    57601 tgtttcttta aattcgtttg cttgattttt cttaaatcca ctttcgcaga gtttttgatt
    57661 gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc
    57721 tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa
    57781 gggtttcttg aatcgctctc cagtcttcca gcccaatcaa tacagcattg tttccactac
    57841 gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa
    57901 aatttttacg agcagtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt
    57961 ctgatataat tatagcgtac ttgattaagt acgtcaaaaa aatacaataa agcacttaaa
    58021 ttcagcaaac ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt
    58081 tgtccacgct cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt
    58141 aacaacgtat catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac
    58201 caatacacga aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga
    58261 tacgcacgaa agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact
    58321 gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat gttcactatc gatttgatac
    58381 ttgcttagac cgttgggttt gtgaatcacc aattaaaaat ggcactgagt tatggtaaca
    58441 aaaaatggtc acttgacgag gctgagattc ctacttatga agacacatgg cacttaagag
    58501 tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg
    58561 gaaattatga tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa
    58621 catttcatac gctgttccca tctttaatgg cttatagagg gcacctttag ctgagaataa
    58681 gacaaaacag accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac
    58741 ttggtttatt tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac
    58801 aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt
    58861 ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt
    58921 acgatatcta cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt
    58981 gaaaaacgga catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg
    59041 acatcttcgt ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa
    59101 ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg
    59161 tcataatcat gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa
    59221 tgatcatgga tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa
    59281 atgtccccta accgatagac aaacaaaatc gcaatgattg catatgagat gaacgcaccc
    59341 gtaatattgg caatcgattt gcttaaccat ctttttcctt cttttttcca tttgacgacc
    59401 atccctgttt gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag
    59461 cgttctgttg agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata
    59521 atcccattgg aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta
    59581 tcttttgcca gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact
    59641 gctaaaatca gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca
    59701 gctttaccaa tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag
    59761 ttgataaatg tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta
    59821 gcagcggcat tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc
    59881 accccagtta aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca
    59941 cccggcacag ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata
    60001 ttatataaac caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat
    60061 aataggaacg atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg
    60121 attgtcacag aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct
    60181 tcagcacctg ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca
    60241 ccggaaatca agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg
    60301 ccatgaggat aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata
    60361 ataacaaaag cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa
    60421 acgacaacga tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat
    60481 gccaaagcag caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta
    60541 cctactaaca acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt
    60601 caagttttat agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta
    60661 aaagtcaata aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt
    60721 caaacgcttt aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta
    60781 taataaaagg atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact
    60841 tttggaagag agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg
    60901 cagttgtcac aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc
    60961 aaccaaatta aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga
    61021 gcagcttttt tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct
    61081 caaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca
    61141 aaacactgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct
    61201 ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag
    61261 acaacaaaca aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct
    61321 cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt
    61381 tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt
    61441 actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag
    61501 acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt
    61561 tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc
    61621 gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt
    61681 cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg
    61741 acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga
    61801 taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg
    61861 tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc
    61921 gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc
    61981 gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta
    62041 ccggtgattt cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct
    62101 aatttttgct gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca
    62161 ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca
    62221 attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct
    62281 ttcgttgagc tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt
    62341 gcagtcccaa tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga
    62401 cctgtattga taccagcaat gatgccattg atattattaa caacggcatc aatttgatct
    62461 agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg
    62521 cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt
    62581 aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt
    62641 gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc
    62701 ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa
    62761 cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg
    62821 cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt
    62881 gcctgatttt ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc
    62941 aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg
    63001 ccgtcgatct ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa
    63061 tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact
    63121 ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc tttattcagt
    63181 ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg
    63241 atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt gatacttact
    63301 aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct
    63361 gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt
    63421 ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata
    63481 tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg
    63541 taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat
    63601 cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta
    63661 ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt
    63721 ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg
    63781 atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa
    63841 tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct
    63901 gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat
    63961 ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa
    64021 ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt
    64081 ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa
    64141 atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga
    64201 ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg
    64261 aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta
    64321 tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc
    64381 cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc
    64441 ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta
    64501 caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg
    64561 ctgaagctcc taattttttt accttttcct catttttact ggatgcactg gttaagattc
    64621 ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta
    64681 gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg
    64741 taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg
    64801 ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag
    64861 ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc acaataccag
    64921 cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta
    64981 aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc
    65041 cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt
    65101 aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt
    65161 atgcctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat
    65221 agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt
    65281 tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa
    65341 agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc
    65401 gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa
    65461 aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg
    65521 tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg
    65581 gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat
    65641 caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt
    65701 tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt
    65761 tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact
    65821 tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc
    65881 gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg
    65941 atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg
    66001 atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg
    66061 ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc
    66121 ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac
    66181 ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc
    66241 atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat
    66301 cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga
    66361 aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca
    66421 ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt
    66481 tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg
    66541 tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc
    66601 gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag
    66661 ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc
    66721 atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact
    66781 ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc
    66841 catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa
    66901 aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc
    66961 aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa
    67021 tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc
    67081 acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac
    67141 tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt
    67201 tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt
    67261 tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat
    67321 actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt
    67381 ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga
    67441 gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa
    67501 ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc
    67561 ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat
    67621 atgcttttct cttttcaata aatttagaac actctcattc taccaaatta taaccatttt
    67681 gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc
    67741 attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg
    67801 tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc
    67861 acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt
    67921 attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc
    67981 atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata
    68041 ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt
    68101 tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg
    68161 tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat
    68221 ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc
    68281 gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac
    68341 acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc
    68401 gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc
    68461 ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt
    68521 acgtccgaag acgattgtta attcaggtgt ttgaacatca aacaagcgag tcatgatatc
    68581 gaatttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc
    68641 tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat
    68701 agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat
    68761 gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac
    68821 taatgtttga acggttccaa gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc
    68881 tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgac ggccaatatc
    68941 tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc
    69001 ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg
    69061 acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc
    69121 tgtctgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgcttg
    69181 gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa
    69241 ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct
    69301 tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca
    69361 cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt
    69421 taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct
    69481 tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt
    69541 tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag
    69601 ttgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt
    69661 tgcttcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa
    69721 cacaggtagt tgggggtatt tttggttcaa ttcttctttt agtgcatgca tttgttcccc
    69781 ataaaggaaa atcatggcga atttctcagg atcgatatgt tcactcattt gcgcatgcat
    69841 atttaaagac tctttcccta attccagcat atctgctaac acagcgattc tgcgtccttt
    69901 tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattgta
    69961 gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt
    70021 gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc
    70081 gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa
    70141 tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg
    70201 aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg
    70261 tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctgatacaa gtccgtcagt
    70321 gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc
    70381 accgatcatt gtgattgctg cagcttttgg ttgagccata agtgaaagtt ccgtgatttc
    70441 attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa
    70501 aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta
    70561 tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt
    70621 aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc
    70681 tttcaaagga tctgctacag gaataactgg aaaatttttt ggagcttgtt caacaggcaa
    70741 gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc
    70801 tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc
    70861 gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg
    70921 tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa
    70981 acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta
    71041 gaatgaacgc ggttacgcaa tcactccagc ctcttcttta tttctcttta ttcatttttt
    71101 tcaaaaaaag attgacgctg atggtatcga ttcattccta actggatcag ttcttcaatc
    71161 aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact gaactccgta
    71221 aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa
    71281 tcgcaccggc tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc
    71341 gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg
    71401 atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc
    71461 gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt
    71521 tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct
    71581 cggttttctg cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc
    71641 ggataaagca aagaaccttc acattgatca aatacttttt taggattttc tttccattga
    71701 ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg
    71761 gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata
    71821 ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact
    71881 ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag
    71941 ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt
    72001 ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga
    72061 acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact
    72121 tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt
    72181 attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata
    72241 acttatttat aaaaaaacga gccagcttct aaaagaacga gcgtcattta ttttttgtca
    72301 gctagattat ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga
    72361 tggcgcaaac aaatccacgc agccgcttgg cagaaatcat tcgagtgttc atcaattaca
    72421 atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag
    72481 aactaggccc caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt
    72541 cttctgcctt cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt
    72601 caacggtcaa aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg
    72661 atttttcaaa atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga
    72721 agaacggtga cgcggtagtc gttaagatcc aacaccctaa catcgccgaa gagatccgac
    72781 ttgatcttca actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg
    72841 tagttgatct aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact
    72901 tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga
    72961 tccgcagccc caagatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa
    73021 tgtctggtaa aaacttgaat catttaatga acgccgaaaa caaaactgaa acttttatca
    73081 ctggaattca aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta
    73141 tgaaacaggt gtttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat
    73201 tccatgtact aacaaaagaa gaacagacac aagctagccg aaaaactgaa acagtccatg
    73261 aaaaagagtt cggttccttt gcttttcgag cctccacttc tgcagaagac cctgttgcgc
    73321 catataccat caactacatc gattttggta tgatggggca tctttctgca ggacttaggc
    73381 aaaaactcac acaagccgtt ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag
    73441 cggtccttcg actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac
    73501 tgacaagttt tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag
    73561 tattcacaca tgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca
    73621 cgcttttgct gaaagcattt gggacattag aaggggtgat ccgcgtcctc gatcctgaag
    73681 tttccctcat ggaagtcgct tcgccttttg ctcaacatta ttttctcact catctggatg
    73741 tagaagatac attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta
    73801 agatccctca acagctccac catctattag aaatgtggac ttcgggacaa ggaaaagtca
    73861 atttggaatt gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc
    73921 ttgttttcgg tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag
    73981 cacctgtaga aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg
    74041 cattcgtcat tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa
    74101 agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg gttttacata
    74161 aattgtttct caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaaa
    74221 caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa
    74281 gggaacatgg aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa
    74341 ataaaatacc ttcgttacct actatcttct gtacttggta aaccttagtc aattgccttt
    74401 tttaacgctt gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc
    74461 cttatgccag cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata
    74521 ttcgcttcgt caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca
    74581 agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg
    74641 attttataat ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg
    74701 tattctttgg tcgtataatg gtccacactg gtcacgataa ccacgtcaag accataggat
    74761 tctcctaatt ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc
    74821 attctttttc gccttcttcc ataaatgata aacagttact aatccgctca gtaaaagacc
    74881 aacattgatc cattttgcca agataagcag ttgtacactt tctatcggca ctgattcatg
    74941 gttcagcgtg atcaataata gatggattgg ctctcccatt tcttttgcta aaacaaacaa
    75001 aagcgtcatc aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac
    75061 aacgatccgg cgatccattg gaatagctgt atcagtttga taatattcct tatttagcca
    75121 gctgcctgtt actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg
    75181 aatcaagagg accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca
    75241 tgttaaacgt ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata
    75301 aggcaaatac ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact
    75361 gctaaataaa aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca
    75421 gcttggcaac ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag
    75481 cagcaagaaa ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt
    75541 tctcaaaaga aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac
    75601 cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg
    75661 gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc
    75721 aggcaactca atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt
    75781 cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg
    75841 tacaagccat gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata
    75901 aaatacgcct aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag
    75961 caacgtgtaa ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg
    76021 aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc
    76081 caaaaataaa agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg
    76141 tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg acaatggctc cttaaacagt
    76201 cttataacta atctgctctt cgattttagc attaaagggg aaataaacca attttacaga
    76261 atatattctt gtaaaaaaaa atacagaaag aagcataagc ttctttctgc atttttacgg
    76321 tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca
    76381 tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct
    76441 ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt
    76501 ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga
    76561 aataagttct tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt
    76621 cccctgattc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata
    76681 ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca aatataattt
    76741 acttaaataa atttttccat ttatctcttc ataagcagtg taaccgacag tcttttcttc
    76801 gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga
    76861 ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca caaaaacttc
    76921 cggccatact tcttgtatga ctgccatcag ctcatccagt tcattttctg ttacttttat
    76981 tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa
    77041 aaagagcatg atcatgctat acacacccac cgaccacggt ttcaagaaaa tcacaatctg
    77101 cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg
    77161 cgcaaaggga agcagcaagg taacaactag tactttttta atatctttcg tttttcttgt
    77221 cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac
    77281 gccatagtaa cggacaagca aaaatccgat tgcttcccca atcactaggc caatacaact
    77341 atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc
    77401 cggcaaaata ggaatgatca cttggatcac ttgcaacaga acaaatatca tcactgcata
    77461 cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg
    77521 ataagcataa agaatcaaca caaagaaaaa aaacagacca ataatgggca tgatctgaag
    77581 acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt
    77641 attaaaactg cctccttatg aactgaaccc caaaagttga actatttaat ggactgtttc
    77701 cgatattcta ctggagatag tccatttaac tttagtttta tccttttgtt attataccac
    77761 ctaatatact catttaattt actttgaaat atctcaattg atcggaattt ttctcgataa
    77821 aaaaactctg atttaagcac accaaaaaaa ttttctatta cagaattatc taagcaattt
    77881 ccttttctag acatgctttg aataatgtta ttctctttta attttttttg atattgtggc
    77941 atctgatact gccatccttg atctgaatgt agaatcagtg aaccctcagt tccctttttt
    78001 ttaattgctt gttgaagcat ttcttcaatc agtttatatg ttggactagt tgatatacta
    78061 taactaatta tttctccgtt aaataaatca agtataggag atagatagat ttttcttcct
    78121 tttatcttga attcagtgac gtctgtcacc cattttttat tgggtgtatc tactgaaaaa
    78181 tttcgtttta acacattctt ggcaattttt cctactgttc ctttataaga tttatatcgc
    78241 tttattcgga tttgacaggt aagtcccatt tgggacatta attttctaac tgttttatga
    78301 ttgattgtat atcctttcat ctttaacgct aaagtgactc tacgataacc ataagagttt
    78361 cttgattctt ttacaatcgc tgtaatttct tgctttatct tgctatattt atctggctta
    78421 tctaattttt ttacccagta ataataagtt gacttcgcta attgtgcaat tgaaagtaat
    78481 agattcaatt taaattcttt tttgagctga aggattgttt taaccttgat ttcttcttgc
    78541 tcaaatcttg ttcttgaatc aaggtttct
//
LOCUS       DAGCXG010000004        74439 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGCXG010000004 DAGCXG010000000
VERSION     DAGCXG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.004"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            181..1473
                     /locus_tag="MB022_000242"
     CDS             181..1473
                     /locus_tag="MB022_000242"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBT4465271.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            2260..3123
                     /locus_tag="MB022_000243"
     CDS             2260..3123
                     /locus_tag="MB022_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4465272.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(3252..4874)
                     /locus_tag="MB022_000244"
     CDS             complement(3252..4874)
                     /locus_tag="MB022_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4465273.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            5123..5644
                     /locus_tag="MB022_000245"
     CDS             5123..5644
                     /locus_tag="MB022_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4465274.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(5830..7560)
                     /gene="spxB"
                     /locus_tag="MB022_000246"
     CDS             complement(5830..7560)
                     /gene="spxB"
                     /locus_tag="MB022_000246"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBT4465275.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            7725..8543
                     /locus_tag="MB022_000247"
     CDS             7725..8543
                     /locus_tag="MB022_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBT4465276.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(8646..8843)
                     /locus_tag="MB022_000248"
     CDS             complement(8646..8843)
                     /locus_tag="MB022_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465277.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            9012..9590
                     /locus_tag="MB022_000249"
     CDS             9012..9590
                     /locus_tag="MB022_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4465278.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            9685..9822
                     /locus_tag="MB022_000250"
     CDS             9685..9822
                     /locus_tag="MB022_000250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465279.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(10635..11138)
                     /locus_tag="MB022_000251"
     CDS             complement(10635..11138)
                     /locus_tag="MB022_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4465280.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            11497..12522
                     /locus_tag="MB022_000252"
     CDS             11497..12522
                     /locus_tag="MB022_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4465281.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            12845..13723
                     /locus_tag="MB022_000253"
     CDS             12845..13723
                     /locus_tag="MB022_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBT4465282.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            14199..14315
                     /locus_tag="MB022_000254"
     CDS             14199..14315
                     /locus_tag="MB022_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4465283.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            15243..15581
                     /locus_tag="MB022_000255"
     CDS             15243..15581
                     /locus_tag="MB022_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465284.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            15675..15905
                     /locus_tag="MB022_000256"
     CDS             15675..15905
                     /locus_tag="MB022_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465285.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            16305..16676
                     /locus_tag="MB022_000257"
     CDS             16305..16676
                     /locus_tag="MB022_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBT4465286.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(16950..17642)
                     /locus_tag="MB022_000258"
     CDS             complement(16950..17642)
                     /locus_tag="MB022_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4465287.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(17645..18430)
                     /locus_tag="MB022_000259"
     CDS             complement(17645..18430)
                     /locus_tag="MB022_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4465288.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            18667..20067
                     /gene="lacG"
                     /locus_tag="MB022_000260"
     CDS             18667..20067
                     /gene="lacG"
                     /locus_tag="MB022_000260"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBT4465289.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            20092..20409
                     /locus_tag="MB022_000261"
     CDS             20092..20409
                     /locus_tag="MB022_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4465290.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            20422..22095
                     /locus_tag="MB022_000262"
     CDS             20422..22095
                     /locus_tag="MB022_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBT4465291.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            22109..23740
                     /locus_tag="MB022_000263"
     CDS             22109..23740
                     /locus_tag="MB022_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBT4465292.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(24231..25796)
                     /gene="guaA"
                     /locus_tag="MB022_000264"
     CDS             complement(24231..25796)
                     /gene="guaA"
                     /locus_tag="MB022_000264"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBT4465293.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25855..26778)
                     /locus_tag="MB022_000265"
     CDS             complement(25855..26778)
                     /locus_tag="MB022_000265"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBT4465294.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26974..27579
                     /locus_tag="MB022_000266"
     CDS             26974..27579
                     /locus_tag="MB022_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4465295.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27887..28903
                     /locus_tag="MB022_000267"
     CDS             27887..28903
                     /locus_tag="MB022_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4465296.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            29044..30345
                     /locus_tag="MB022_000268"
     CDS             29044..30345
                     /locus_tag="MB022_000268"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBT4465297.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30391..31053
                     /gene="deoC"
                     /locus_tag="MB022_000269"
     CDS             30391..31053
                     /gene="deoC"
                     /locus_tag="MB022_000269"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBT4465298.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            31074..31472
                     /locus_tag="MB022_000270"
     CDS             31074..31472
                     /locus_tag="MB022_000270"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBT4465299.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31538..32620
                     /locus_tag="MB022_000271"
     CDS             31538..32620
                     /locus_tag="MB022_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4465300.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32832..34394
                     /locus_tag="MB022_000272"
     CDS             32832..34394
                     /locus_tag="MB022_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465301.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34387..35520
                     /locus_tag="MB022_000273"
     CDS             34387..35520
                     /locus_tag="MB022_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465302.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35520..36476
                     /locus_tag="MB022_000274"
     CDS             35520..36476
                     /locus_tag="MB022_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465303.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36575..37741
                     /gene="deoB"
                     /locus_tag="MB022_000275"
     CDS             36575..37741
                     /gene="deoB"
                     /locus_tag="MB022_000275"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBT4465304.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37765..38583
                     /locus_tag="MB022_000276"
     CDS             37765..38583
                     /locus_tag="MB022_000276"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4465305.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38600..39307
                     /gene="deoD"
                     /locus_tag="MB022_000277"
     CDS             38600..39307
                     /gene="deoD"
                     /locus_tag="MB022_000277"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4465306.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39605..40291)
                     /gene="gpmA"
                     /locus_tag="MB022_000278"
     CDS             complement(39605..40291)
                     /gene="gpmA"
                     /locus_tag="MB022_000278"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4465307.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40463..41044)
                     /locus_tag="MB022_000279"
     CDS             complement(40463..41044)
                     /locus_tag="MB022_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4465308.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(41177..41860)
                     /gene="rpiA"
                     /locus_tag="MB022_000280"
     CDS             complement(41177..41860)
                     /gene="rpiA"
                     /locus_tag="MB022_000280"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBT4465309.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            42179..42592
                     /gene="rpsL"
                     /locus_tag="MB022_000281"
     CDS             42179..42592
                     /gene="rpsL"
                     /locus_tag="MB022_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBT4465310.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42676..43146
                     /gene="rpsG"
                     /locus_tag="MB022_000282"
     CDS             42676..43146
                     /gene="rpsG"
                     /locus_tag="MB022_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBT4465311.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            43240..45327
                     /gene="fusA"
                     /locus_tag="MB022_000283"
     CDS             43240..45327
                     /gene="fusA"
                     /locus_tag="MB022_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4465312.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45499..46686
                     /gene="tuf"
                     /locus_tag="MB022_000284"
     CDS             45499..46686
                     /gene="tuf"
                     /locus_tag="MB022_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4465313.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            47093..47401
                     /gene="rpsJ"
                     /locus_tag="MB022_000285"
     CDS             47093..47401
                     /gene="rpsJ"
                     /locus_tag="MB022_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBT4465314.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47432..48061
                     /gene="rplC"
                     /locus_tag="MB022_000286"
     CDS             47432..48061
                     /gene="rplC"
                     /locus_tag="MB022_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBT4465315.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            48089..48712
                     /gene="rplD"
                     /locus_tag="MB022_000287"
     CDS             48089..48712
                     /gene="rplD"
                     /locus_tag="MB022_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBT4465316.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48712..49002
                     /gene="rplW"
                     /locus_tag="MB022_000288"
     CDS             48712..49002
                     /gene="rplW"
                     /locus_tag="MB022_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBT4465317.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            49041..49874
                     /gene="rplB"
                     /locus_tag="MB022_000289"
     CDS             49041..49874
                     /gene="rplB"
                     /locus_tag="MB022_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBT4465318.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49917..50195
                     /gene="rpsS"
                     /locus_tag="MB022_000290"
     CDS             49917..50195
                     /gene="rpsS"
                     /locus_tag="MB022_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBT4465319.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            50218..50565
                     /gene="rplV"
                     /locus_tag="MB022_000291"
     CDS             50218..50565
                     /gene="rplV"
                     /locus_tag="MB022_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBT4465320.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50579..51235
                     /gene="rpsC"
                     /locus_tag="MB022_000292"
     CDS             50579..51235
                     /gene="rpsC"
                     /locus_tag="MB022_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBT4465321.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            51238..51672
                     /gene="rplP"
                     /locus_tag="MB022_000293"
     CDS             51238..51672
                     /gene="rplP"
                     /locus_tag="MB022_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBT4465322.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51662..51850
                     /gene="rpmC"
                     /locus_tag="MB022_000294"
     CDS             51662..51850
                     /gene="rpmC"
                     /locus_tag="MB022_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBT4465323.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51875..52141
                     /gene="rpsQ"
                     /locus_tag="MB022_000295"
     CDS             51875..52141
                     /gene="rpsQ"
                     /locus_tag="MB022_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBT4465324.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            52199..52567
                     /gene="rplN"
                     /locus_tag="MB022_000296"
     CDS             52199..52567
                     /gene="rplN"
                     /locus_tag="MB022_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBT4465325.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52604..52912
                     /gene="rplX"
                     /locus_tag="MB022_000297"
     CDS             52604..52912
                     /gene="rplX"
                     /locus_tag="MB022_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBT4465326.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52940..53479
                     /gene="rplE"
                     /locus_tag="MB022_000298"
     CDS             52940..53479
                     /gene="rplE"
                     /locus_tag="MB022_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBT4465327.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53498..53683
                     /locus_tag="MB022_000299"
     CDS             53498..53683
                     /locus_tag="MB022_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBT4465328.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53719..54117
                     /gene="rpsH"
                     /locus_tag="MB022_000300"
     CDS             53719..54117
                     /gene="rpsH"
                     /locus_tag="MB022_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBT4465329.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            54149..54685
                     /gene="rplF"
                     /locus_tag="MB022_000301"
     CDS             54149..54685
                     /gene="rplF"
                     /locus_tag="MB022_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBT4465330.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54846..55202
                     /gene="rplR"
                     /locus_tag="MB022_000302"
     CDS             54846..55202
                     /gene="rplR"
                     /locus_tag="MB022_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBT4465331.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            55223..55723
                     /gene="rpsE"
                     /locus_tag="MB022_000303"
     CDS             55223..55723
                     /gene="rpsE"
                     /locus_tag="MB022_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBT4465332.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55738..55917
                     /gene="rpmD"
                     /locus_tag="MB022_000304"
     CDS             55738..55917
                     /gene="rpmD"
                     /locus_tag="MB022_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBT4465333.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55961..56401
                     /gene="rplO"
                     /locus_tag="MB022_000305"
     CDS             55961..56401
                     /gene="rplO"
                     /locus_tag="MB022_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBT4465334.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56401..57696
                     /gene="secY"
                     /locus_tag="MB022_000306"
     CDS             56401..57696
                     /gene="secY"
                     /locus_tag="MB022_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBT4465335.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57758..58405
                     /locus_tag="MB022_000307"
     CDS             57758..58405
                     /locus_tag="MB022_000307"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBT4465336.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58596..58814
                     /gene="infA"
                     /locus_tag="MB022_000308"
     CDS             58596..58814
                     /gene="infA"
                     /locus_tag="MB022_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBT4465337.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58847..58963
                     /gene="rpmJ"
                     /locus_tag="MB022_000309"
     CDS             58847..58963
                     /gene="rpmJ"
                     /locus_tag="MB022_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBT4465338.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58981..59346
                     /gene="rpsM"
                     /locus_tag="MB022_000310"
     CDS             58981..59346
                     /gene="rpsM"
                     /locus_tag="MB022_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBT4465339.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59374..59763
                     /gene="rpsK"
                     /locus_tag="MB022_000311"
     CDS             59374..59763
                     /gene="rpsK"
                     /locus_tag="MB022_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBT4465340.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59843..60781
                     /locus_tag="MB022_000312"
     CDS             59843..60781
                     /locus_tag="MB022_000312"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBT4465341.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60809..61189
                     /gene="rplQ"
                     /locus_tag="MB022_000313"
     CDS             60809..61189
                     /gene="rplQ"
                     /locus_tag="MB022_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBT4465342.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61461..62300
                     /locus_tag="MB022_000314"
     CDS             61461..62300
                     /locus_tag="MB022_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4465343.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62276..63145
                     /locus_tag="MB022_000315"
     CDS             62276..63145
                     /locus_tag="MB022_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4465344.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            63135..63932
                     /locus_tag="MB022_000316"
     CDS             63135..63932
                     /locus_tag="MB022_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4465345.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            64135..64881
                     /gene="truA"
                     /locus_tag="MB022_000317"
     CDS             64135..64881
                     /gene="truA"
                     /locus_tag="MB022_000317"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBT4465346.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(65336..68005)
                     /locus_tag="MB022_000318"
     CDS             complement(65336..68005)
                     /locus_tag="MB022_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBT4465347.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(68243..68431)
                     /locus_tag="MB022_000319"
     CDS             complement(68243..68431)
                     /locus_tag="MB022_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBT4465348.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68701..69063
                     /locus_tag="MB022_000320"
     CDS             68701..69063
                     /locus_tag="MB022_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4465349.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            69115..70797
                     /locus_tag="MB022_000321"
     CDS             69115..70797
                     /locus_tag="MB022_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBT4465350.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70914..72950
                     /gene="recG"
                     /locus_tag="MB022_000322"
     CDS             70914..72950
                     /gene="recG"
                     /locus_tag="MB022_000322"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBT4465351.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72999..74000
                     /gene="plsX"
                     /locus_tag="MB022_000323"
     CDS             72999..74000
                     /gene="plsX"
                     /locus_tag="MB022_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBT4465352.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            74122..74364
                     /gene="acpP"
                     /locus_tag="MB022_000324"
     CDS             74122..74364
                     /gene="acpP"
                     /locus_tag="MB022_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4465353.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        23732 a        13339 c        15859 g        21509 t
ORIGIN      
        1 tttcttgaag ctgaccactt ctgttacaac ctctttttta ataaccattc taaaaagata
       61 aagttcgttt tttaagcgtt tttataaaat aaaaataaaa atcattcgat tttatttgaa
      121 atatgtatct attattgata caatggttca ggattaaata atatagaacg aggtgttcac
      181 atgtcatctg tagtagtagt aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca
      241 gactttctca gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga caatgcaggg
      301 cacacaatta agtttaacgg tgtgacttat aaactgcatt tgattccttc tgggattttt
      361 tataaagaca agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa atcattggtt
      421 aaagaattag cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac
      481 cgcgcgcacg taatcttgcc ttatcatatc aaattggacc aattacaaga agatgctaag
      541 ggcgaaaata agattggtac aacgattaaa ggaatcggac ctgcctatat ggataaagca
      601 gctcgtgtag gtatccgtat tgcagactta ttagataaag aaatttttga agaacgtctt
      661 cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt
      721 gattttgacg atatttttga agaatattat gaatacggac aacaaatcaa acaatacgtc
      781 acagatactt ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa
      841 ggcgcacaag gtgtaatgct ggatatcgac cagggaactt atccttttgt tacttcttct
      901 aacccagtag ccggtggggt aacaatcgga agcggtgtag ggccttcaaa aatcaataaa
      961 gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt tccaactgaa
     1021 ttatttgatg aaacaggaga acaaatccgc gaagtaggac gagaatacgg tacgacaact
     1081 ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc taaacgagtt
     1141 tcaggtatta ccaacctatc tttaaactcg atcgatgtat taagtggatt ggaaacagta
     1201 aaaatttgta cggcttatga attagatggc gaactaatct accactatcc agcaagcttg
     1261 aaagagttga accgctgcaa accaatctat gaagaattgc caggctggtc agaagacatt
     1321 acaggatgca aaacacttgc agaactgcca gaaaacgcac gtaactatgt acgtcgtatc
     1381 tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg gacctgatag aacacaaaca
     1441 aacatcttag aaagcgtctg ggcgcaaatc taaggataaa aacgacgact gaatcaggac
     1501 gttcttacca gagcgagtcg aagtgtttat ctatacgata ataataactt gaagaggtag
     1561 tgaaagagac gtttagcctt attgccgata ggctgtctct tgaacactgt ttattcagaa
     1621 taagggactg tgacaaaaac cgtgtcacag tccctttttc cgaacaaacg gtgttcaaaa
     1681 gcttgcatct acggcactaa gcccaatcag atgaaaaata tggagtgcta tttttatctg
     1741 attgttctta ctgcttgatg cagagcagct ttttcacaac ctctccacta aacggtgttc
     1801 tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgttttttg
     1861 aaattgttcc ttgtgactcg gagctaaacg gcaggctcac aacctcttgg taaacggtgt
     1921 tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttt
     1981 ccgttttttt ctctaatact caggtcaaaa cgctgatttc acaacctctg attcacggtg
     2041 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaatttga gaagcaattt
     2101 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc ttattttgat
     2161 tagaacttgc gaaaataaac cggttaaagt gtgtctagcg caagagattt gctataatag
     2221 acgataaggg taaaatgtaa cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat
     2281 tcttgttgtg atctgccata ccatgtatta gcaaaagaaa aagttgattt tttatcaatg
     2341 aatctccaat tgaatgatca agagttagta gatgacctcg gaaagacatt caattatgac
     2401 cgctacatga atcagctgaa aaatggtgcg atgcctacga catcacaaat caatgttggc
     2461 cgatatttag aatttttccg ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct
     2521 ttttccagtg gattaagcgg ctcttacagt agtgcgcttc aagcggttga attgctgaag
     2581 gaagaatatg aagaccctga gatctatgtt tttgatacaa aagctgcaag tattggggaa
     2641 gggcttttag tattggaagc tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc
     2701 ctagagtggc tggaaagtaa ttatttgaaa gtccattcat gggtaacagt agacgatctg
     2761 aaatatctag aacgaggcgg taggatttcc aaagcaagtg ctactcttgg cagtctattg
     2821 agtgtaaagc ctattattac ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt
     2881 cgaggaagaa acaagtcgat ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca
     2941 ccacttgatc aaatcgttta tgtggcttac gctggggatc tagagagcgc ggagaaggca
     3001 aaacggcttt tggaagagca gataaagatg aaagatgtga agctttatcc cttaggacca
     3061 acgatcgcca gtcacacagg ctatggctgt atcgcgattt tttcgatggg aatcagtcga
     3121 taaaagcagt aactcattct gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac
     3181 tcttaaaaga tagatgagca tcctatctct tagaggtcgc tgttttttta ttttttaagc
     3241 taaataggcc attatgccca taactcgttc aattgttttt gaatatctgc attttccaaa
     3301 aattcatcat atgtcgtatc agctcgatcg atcacacctt tatccgaaac ggcaatgatg
     3361 cggtttgcca atgtttggat aaattgatgg tcatgtgacg caaataagat tgagccagta
     3421 aatgccatca agccgtcatt caatgctgtg attgattcta agtctaaatg gttcgttgga
     3481 tcatctaata caagtacgtt agctttcgaa agcatcattt ttgaaagcat gcaacggact
     3541 ttctctccac cagataaaac attcactggt ttcaatactt cttcaccaga aaacaacatt
     3601 cttcctaaga agcttcttaa gaaggtgtta tcatcttctt ctttacttgc atattgacgc
     3661 aaccagtcta aaatcgttag atcagtatcg aattctttcg ttgtatcctt tggtaaataa
     3721 gaattacttg ttgtcacacc ccaacgtact gttccagtat ctggcgtaat ttcacccatg
     3781 ataactttga atagcgttgt tgttgttata tcattttctg cgacgaaagc gaccttgtct
     3841 tctttgctca aattaaaaga tatatcatct aaaattttct ttccatcaat cgttacactt
     3901 acattttcta cctgcaataa gtcgtttcca atctcacgtt caggtttgaa tccaacgaat
     3961 ggataacgtc tagaagaagg ttggatatcg tctaaagtaa ttttttcaag cattttttta
     4021 cgtgaagttg cttgttttga ttttgatgca ttcgcactaa agcgggcaat aaattcttgg
     4081 agttctttga tttgttcttc ttttttggca tttgcgtttg cttgaagttt agccgctagt
     4141 tgactagatt ccaaccaaaa atcataattt cctacataaa gtttgatttt accaaaatct
     4201 agatccgcca tatgtgtaca tactttgttc aaaaagtgac ggtcatggga tacaacgatc
     4261 acagtatttt caaaattgat caaaaattct tccagccaat tgatcgattg gatatccaga
     4321 ccgtttgttg gttcgtctag caaaagaaca tcaggtttac caaatagtgt ttgtgctaat
     4381 aacactttta ccttttgtcc accagttaat tcactcattt gctgatggtg caattcttca
     4441 gggatgttca gaccttgcag taaaacagct gcttctggtt cagcttccca tccattaagc
     4501 tcagcaaatt ctccttctaa ttcagctgct cgaataccat cttcgtcagt gaaatcctct
     4561 ttcatgtaaa tcgcatcttt ttctttcagc acttcataca gacgcttgtg tcccatgatg
     4621 actgtttcaa gtacagtata gtcttcatag tcgtaatggt tctgtttcaa tacggccatc
     4681 cgttcatctg gtcccatagt aacggaacct gtggaaggct gcagatcccc tgataacact
     4741 ttaaggaacg ttgatttacc tgcaccattt gcaccaatca agccatagca gtttccgggg
     4801 gtaaatttaa tattcacatc atcaaatagt ttgcggtctg aaaagtgcaa actcacatca
     4861 tttacagtaa tcaatttttt tcctcacttt tatcattatt agttttgaca agtcttatag
     4921 aattatatag gggaaaggta aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg
     4981 aaacagaatg aaaaaaataa aaataaaagc ggagcgaaaa tagctactat aggatttatt
     5041 agtttgcaac agcataattt ttgacgttat ttataagagg gagtactata aaactgtaaa
     5101 agaaatgaaa gaaggattga aaatggatga aaaaagaacc atcgactggg gctcattgat
     5161 tttgggaatt ttatttgtac ttgtctcatt gttatcattt caagaccctg tcggaaattt
     5221 agttgcaatt gtagtagtct ttgcgatttt tgcctttatt aaagggatat ttgaactgtt
     5281 tgtacggaac agattaaaag agctaacagg atataaagga aagatgccct taataatcgg
     5341 gatcatcgat attctagttg gcgtcttttt cttattcaat attggtgcag gagtcgcagc
     5401 attgcctttt gtttttgcag tatggtttat tgctgattca gttttagcgt tatttactgc
     5461 tgacttggca agaggagtaa gtgaaggata ttattggttt acgatcatcg tcaatatttt
     5521 aggaatttta ttaggaattt tcttattgtt taatccaatt tcttctgcgt tgacattaag
     5581 ctttttagtt ggattctatt ttatgttatt tggtatcaca catatcgtct atgcatttag
     5641 ataagtaaaa tcaaacaaat aaaaaagccg gcttccgatt acagttttac tgttgtaatt
     5701 ggaagccggc ttttttatca tggacaaata tgcacgaggt gatttttagg tgtcgagaca
     5761 aatttatgca aaaatgtgag aagctatata cacaaatttt atcttaatac tcgaagcata
     5821 gcgcagttgt cacaacctct tttcatataa ttcattcaat actggcaaat catggacttc
     5881 atatttttct ttgaatgctt ggatttcttc ttcagaatat tttcttggat ctaaatgcag
     5941 atcttctact ggcaatgggc gcttgttatt gatcttcaca tcgattacta caggacgatc
     6001 tgattttgca gctgcatcaa aggcaaatcg aagttgcgag tattctgtga ctgtaaagct
     6061 atttgccccg agcgcttctc caactttacc gaaatctgca tcttctaaaa atacaccaaa
     6121 tttcttttgt tccgtatctt cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa
     6181 gacaacatta ataattggaa gctgatattt gacttgtgta atgatatctt gcatgaccat
     6241 agcaaacccg ccatcaccac ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc
     6301 ggcaattcct cctggcacac cattacccat agttgcgaac catccagatg tcgtatgttt
     6361 ttgtttaccg ttcatattca ataaacgaat agaatggata gtcgtgttac cgacatctgt
     6421 gacgaaaatc gcatcgtctt cagcaatacg attgatttct ttatagacag cctctggacg
     6481 aatcggttct tcctctcgat cttcaaaaga tttcaaccaa tttacccatt tttctttatt
     6541 ttcttgattt gctaggtacc agctatcagc agggcgagca tcccctaact cgacaagtct
     6601 ttgcaaagct gtgtttgcat caccgaggat cgacaaagat gtttcatggc gtcgtccaaa
     6661 ttttgacgca tcgatatcga tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc
     6721 aaatgggaag tcgctaccga caaataagat cagatcggct tctgccaatg cttcatttcc
     6781 gggtttagta gcgacacgtc cagcaaaacc aagaaagttt tcataatcat caggaataat
     6841 tccttttgct agaacggaag cgacaacagg aactgaaaaa tgttctgaga attctttgat
     6901 ttgcggaaat ccgtttcgcg ttccttgacc aacataaaga actggttttt ttgctttttc
     6961 aagataaggc aaagcagcta gcaaatcttt ttcttctggt aatatcacac ttgttttgtg
     7021 agtgtgagcg gtggagaaag gttgttcgtc aatttcttca aaaccaagat caacagggat
     7081 agtcacgata gccacgcctt tatgctcata ggcggcttta atggcttcat ctactacatg
     7141 cggaagactt tgtggtgtca tgactgtacg attgtatacg ctaacatctg cgaaaatagg
     7201 attttcattt agttcctgga aagagttata gttcatggat gtagaagcaa cttgtcctaa
     7261 gaacgctact agaggcacat ggtccatctg tgcatcatac aaaccattga tcaaatgtgt
     7321 cgcaccaggt cctgcagagc cgaagacagc gccgactttc cccgttaatt ttgcatctgc
     7381 agcggctgcg agtgcgccga cttcttcatg acgaacttga atgtagttga tttcggtttt
     7441 ttcttgatat agcgcatcca taattgaatt gaaagatcct ccgggaatgc cgtaaatatg
     7501 gtcgataccc cagctttcaa gaacttttac cattgcgatt cctgcattga tttttgacat
     7561 ggtgtttcac tcctttagca atgatgatac ataaattttt tgtatctaga gtatagaact
     7621 ttttatcgaa aatcccaact aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa
     7681 tatgctagag tagtacatag atagattcaa gagaggggaa tggcatggtt gaatggctat
     7741 tgagacttga accttggcag caagcacttg ctgggacagc ttttacttat tttatgacag
     7801 ctcttggtgc cgggttggtt tttttcttta aagaaatcaa aaaagaagtg ctaaacttga
     7861 tgttaggatt tgcttctggt gtcatgattg ctgcaagttt ttggtctttg ttagatccgg
     7921 ctattacaaa agcagaagaa aatggagata ttgcttggct agttgtaagt attgggtttg
     7981 gtttaggcgg tgtgttttta tatatggcag ataaaacact gccccatatg cattttggac
     8041 cacaacatga agcagaagga ttgcctaccc atttaaaacg gacgatcttg cttgtttttt
     8101 ctattacatt gcataacatt cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag
     8161 caactgcgga taatccgaca gccgctatat tagccgctgt ttcggtggcg ctaggtattg
     8221 gtatccaaaa cttcccagaa ggagcagctg tatcaatccc gttgcgacaa gaaggtctga
     8281 gtcgtaaaaa ggcctttgtt tacggacaag cttctggaat cgttgaacca attgctggag
     8341 tgattggagc gctactagta acgaaagtgg agctgctgct tccttatgca cttgcttttg
     8401 ccgcaggagc aatgatttat gtcgtggtgg aagaattgat ccctgaagca cagcagacat
     8461 tgtccagcaa acgtcatttt gcagtttttg gcgtcatgag tggatttatc attatgatga
     8521 ttttggatgt ggcattagga taaataaaaa aaatatatat gatatatcaa atagggccgc
     8581 gaacttatat cgcgacccta tttttttagt aagttatatc ataatgaagt gaatggtatc
     8641 atacattaat aatagttttt tctatgtgta tttcctttga tccagcgaac aagccagatg
     8701 ataagtgcga ttgggattag aactttgacc gcaaaccata aaagcccgct taggacactg
     8761 acaaaaagac tgatgacgaa gctagctgca attagcacta aaagaaccat gaagatattt
     8821 aagaacatat ttttttgacg catacaaaac cctcctcttg ttcattcttt actatagaaa
     8881 aagtataaca gggattgttt aaaaacacct ccaactgaag actgattttt acttcatcta
     8941 cttaaatgat caaacaattg ctttttctct ggtttatcat ctaaaataga gcggaaaagg
     9001 aggagaatat catgcctaaa aatcgtcttg aagcatttac agatgctgta atcgcgatta
     9061 tcatgactat tttagtttta gaacttcatg aacctaacgg agacacgtgg gaggcgctcg
     9121 ctgcattagg ttaccgattt tttatctata ttgtcagttt tatcatttta gcgatttatt
     9181 ggaacaatca tcatcattta tttcaagttg tacataagat aaatggacgg gtcttatggg
     9241 cgaacagttt ctttgttttt gcgctttcgc tttttccgtt tgcaacttca tggatcagtg
     9301 agtacatcaa tagcctggct ccagaaatga cttatggttt tgttaccttg accgctaata
     9361 ttgcgtatta tatcttggca aaagaacttg tcaaagcaaa taaaggccac gagaaagcat
     9421 tgaaggcgat gttgaataat tacaagaaat cgtatctatc gattggtttg aatgtgttag
     9481 gaattgtctt agggtattta gtagcgccag tagccgtgct gatcgcaaac atcgtgattc
     9541 tagctgcttg gatcatccca agtcgtacca tcgaatcaca atatcggtaa aatgatccgt
     9601 gtttaaaatt taagagaaaa atatagaaaa aataaataaa cgatcaaata tccgagtgat
     9661 actagtattc aaagagggat gcagatggat caaaaaagaa ttcatgtagg actgattact
     9721 agttttgtca cgatgcttat ttatttggat caaggtcatt tgacttggac agtagttggt
     9781 gtcgtaggat ttttgacatt attcacagca ataaaaaaat aaacatcttt atgtaatctg
     9841 atgttctatc tatttaagaa aagcatgttt acagcagtat gattctgttg taaacgtgtt
     9901 tttatttgtg gaaaatttaa tttttagaaa agtgtatcga aaaatagata gttagttttt
     9961 ccacagtgga taacttcgta agagataaaa ataaaaaatt agtcaaagaa gggcgtatag
    10021 atttcttttg ataggagata tacaacagaa atgaataaat attaacgtgt tttcaaacag
    10081 tataaaacgg tgtggttttt ggaataaaaa aataacatat ctcattttac acttttgtta
    10141 ttttatactt aaaaaataac gacacagaaa ataccgtgtc tcagatttct ctactacttc
    10201 taaaaactac tgtttttttc ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata
    10261 ctggcagaat caaggagaag ggagcctaaa taaggagaaa aaccatagaa aatataaagt
    10321 aattacttat aaaacaagct ttttatagaa aaataatata cctgagtagg gtataaatta
    10381 agacggaatt cctttctaga ttgagatatt gactaggaag aatgttaaga ttttaagcct
    10441 ttaagctaaa aaaaaaccct ttttgttaaa taaggggaat atagcctgtc agtttcaaac
    10501 tgcaagtcgt cctgctgcaa taatatagta gattagagat gaaaatagat ttttttaaac
    10561 caatgactga ataaaaagac cgtcatagat acacattgga atgtgtctat tacggtcttt
    10621 ttttacgaac gattttattg aagacggaca gttcgggtca aaaataaaaa agctacgatc
    10681 agaaataaga ataaaatgta tcccaaaata atggctagac ggtctaactt atttgtcgat
    10741 agatgtttgt ttagaagttg ataggcccat aaacctatca gtcctcctgt tgtattagtt
    10801 atcaaatcag tgatgtccgc tacgccaata ccgaatagga attgaatcaa ttctgccaag
    10861 aagcttacaa ggaaaataat ccctaatttt ttagaaaaag aaagtctctt gaaattgaca
    10921 ttcaaaagca ccccaaaagg aaaaaagaca atcaagttat aagtggtctc acgtaatacc
    10981 gtcgtatttt caaaagaaaa agtagaaaaa gggacgagat tgacattgct gtaagaataa
    11041 gtgagaaatt gtggaagatt aaatgatagc ttgaataaaa tcaaccataa tagaaagaat
    11101 aaataaatcc ctaataaaaa ttttgataca taccgcatag acatctcctc catttaaaaa
    11161 gtataccatc gaaaaccgct gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta
    11221 gttatcgtac gaaaattaga agcttttttt tgttgggaat ccggtttctt taacaaagct
    11281 aagatagcga gttgcttgga aaagttgcta tcttttttct gtcaaaacga ctcggaatgt
    11341 tcgttttttg ttctaatttc tccaagttgt ttactttttg atactaagtt tatgaaaaat
    11401 caagaaatag ttgttatact aggtaatgct gacaaacggg attggcattt catcttggtt
    11461 cagctttttt tccaattact aggaaggagg gatagattgt tcaagactta ttttcgtttc
    11521 aataaaatac aagataatcc ttttcgtatc atgaatctga tgctacttat ctttattgta
    11581 ttatggttag gttacataaa tcatgatttt ttgatttcaa gtttttcatc attagggatc
    11641 tttacttttt tctattatca aaacataccg atgaaatatt tgctgaaacg tatgtttctg
    11701 atcggttgcg gactgttggt tgctttcgtt ttggggatct tatcgactta tgttttatgg
    11761 cttgaaccat ttgccgtagc tttggttgct ttttccagca gattcgtgtt gcgattattc
    11821 catatttcta aaccaggtgg cttgtttttt gcgatgcttt ctgccatggg aacgagcatg
    11881 cagctgccaa tcgctcaatt accgatcgtc tcactttatt tttttatggg tgtcgttttt
    11941 gccctgattg cggcagtgat tacaaaatta ctggattcac ggcctgagca gacaattgaa
    12001 aaagcaactt taaaagaaag atttcacgaa gagcctttag tgatcattga ttctgttttc
    12061 tacagcgctg ccttatttct ttccgtttat gtcagtcacg gcttgaatct gcataatcct
    12121 tattggttga ccttgtcatg tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag
    12181 catcgacaag tccaatatct gattggatcg atgggtgggt tatgtgtgtc ggcattcttg
    12241 tcatttgtgc cgtttacgca gttgcagacc atttttctga tcacttttct atatggaatc
    12301 gcacaatttc tagttgctag aaattatgca gttgctaata tttttcttaa tccgatggcg
    12361 ttgatgctat ctacacttat tcgtggagca tacttgattt ctttgatcga atatcgtttt
    12421 ttagggatcg tcataggaag tttcatcggt ttaggtgttg cgtgggtaat gacggttggt
    12481 ttgcaacatt atttaacggt tgttcgtcaa aaattagaat agaaggacaa atatatgtgc
    12541 caaaagaggt tatgacaaga tttttgtcac aacctctaat tcaaggtgtt caaaagccag
    12601 attctcagca ttaagtcaaa ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt
    12661 atatttattc tatcaaaaaa ttttgtttat gatggtaact gaataaactt tcctgagatg
    12721 gtcaatgctt tccttgaaag tcttacagat attctttctt catagaaagt ttaagtttta
    12781 tagttagtgt acaataaaag tatgtttata ccatatggtg tccggataat cagtcattta
    12841 aacaatgagg ttcaaagata tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat
    12901 ccaaattctg gcagaaactt taactcttcc caaagtcgtt atagagcaaa tgattgtaca
    12961 agcaaaaaac atccttgatg aggtgtttga gcaatatagt catgaactaa tggatcctaa
    13021 taaagcaaag gcgactgcaa agaaaataga caaagtctat gaagaggaac cttttgcagc
    13081 attagctatt tatctatatg cagcttctca aagttggaaa aaaatttatc aaccattgaa
    13141 gattccaaga actatctatt tggcaacaat gaatgctttt actagattta ttcatgaaca
    13201 tttccaagta acaggaagct atcgctttga ccgtggtttt tggatttggc attacactag
    13261 tggcttgatc tttcggattg gtgaattgga gtacgaaatg acatattttc cgaaagggta
    13321 cacggatcta agattagaag gaaagcagtg cctttctctt catattcctt ccgatgcaga
    13381 tttatctcag aataagataa gagatagtta cgaaagtgcc attcgttttt ttgaacttta
    13441 ttttcctgat taccattacc aagtgatgta cacagatact tggttactat cacccaatct
    13501 tactaaatgg ttgaaaaaag agtcaaaaat ctgtcttttt gcagccgatt atcgattatt
    13561 atcagttgat gaacaggacg atagtggagt cccttggatc tttggacgag tagatgccca
    13621 aatacatgat tatccagaaa gtacgagttt acagcgacaa gcaaaagagc aattgcttgc
    13681 aggcgagcac attggctctg gcttaggtat tcttacttca taagtttagt acttcgaaat
    13741 agtttaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    13801 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
    13861 aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    13921 tttttagtgt tctatcgaac acacagtacg ttcaataggt aaactagcta taacaataga
    13981 tattaaagtt aacggacaag agggcctttt ttaggtagct aaaacgtaat aatgaaaatt
    14041 tatttccaga gataggagtc aatcagctag tctgatcgac tgttttaaca agttgacttt
    14101 cattttaact tttgatacca tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt
    14161 agcatgacta caatcaaaga agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa
    14221 gtgcataccg ccgcttgaaa agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca
    14281 tcaaagatgt gaaacggaat agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa
    14341 tagccgaaaa acgtttttcg gctattttca ataatcggtt tttgtgtaag gtttccgaaa
    14401 gaactgctgt tgattttcca tttttaacat atgataaatt ggtagaatct ttcttttcaa
    14461 attgatacca ttccctaatt ccagttatat agttaactac agaaccacaa agcacgtacg
    14521 tttataaaag tattatctgt agttattttt caattccctt tcaacctctt attttgacca
    14581 gtagtattga tcactttacc ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa
    14641 gaatggaaac gtaactacat aatcaaaggt tttgcgaaat atttcatgaa atatttcatg
    14701 tagaagcgag caatacggtt gcaaataacc attatcgttt aataagcagt tacgaaagct
    14761 cccaatatct ctaacgttca gctcactcca tttcttttct tcaaaaaaat cagtgattcg
    14821 tatctctttc tatttgtcga aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa
    14881 aaaaataaaa aagcagttac tattgctaga ataacatcaa ggaataataa gttttggttt
    14941 tcagataata gtcgataagt tgtgtattgg aattttagat acagtaataa agtatttttc
    15001 atgaaatttt gtgagaatca ctattacaag tatctttaat aaaattaatg gttaaggagg
    15061 aacttaaagt agaatgtaaa tgaatagttg tagtcagtcg attgtgatac tagactcaaa
    15121 gttgcaggaa taagtttttc aatttgtgat tggaagataa aaaatgaaaa agaagggctt
    15181 ttttttatac tttgttgatt gtagtgttta ttttccctct gtttagccaa gtaacatttt
    15241 ctatgactca accagtttca agtgaggaac atgataacct ttttcgtgct acagaagagt
    15301 ttaagaaata tcaggaaaat attgttccgg agactccatt agctagtgag ccaattactg
    15361 atattgatgg aaaccaaata ggatggtata tacagtacga agttatggtg gtaaataatg
    15421 actcaatagt aaaaaataca gtaggcttag agctaacacc gattgaaaag gtgagttcat
    15481 tgttaacgta tatttatact gatgaaagtg acgagactga gatcatttta acggattact
    15541 caaaaattgc tactgataaa aagtatatat tgtggatgta gacactagtg aaatacttta
    15601 tgatatagaa attgatgaaa atttactaga tgtagcacaa gaaatcgatc atcaaaagca
    15661 ggaagctatt cgatatgctg gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag
    15721 caaaagttag agaagatgaa aatgagtgga aagaattcaa gagtcgctac tcaattaact
    15781 cgactccgac atttacagtt tacagagaag gatcgataga aaaaactgtt ttttggacta
    15841 aagaaagtgg aatgtcttta gctgaagtag aagagttttt ggattatgtt agtatgcaac
    15901 agtaagggag ttcttattat gaaaaatttt gacacatatt atgttattgt tttttcgata
    15961 tctttgctgt taatattact aactttaatg cagtataaag agtatccaga gtattttatt
    16021 ttaacttttg tatatttagt aagtagctac acacttctat ataaattcag aagttcttct
    16081 agagtttcta aagtttgttt cttaattagc ttattgattc tataatattt ttgatttata
    16141 taggaaatta aaaatattat agaatcaaca ctcagagaac gggttggttt ttaatagatt
    16201 taataagtac tgataatgaa atttctcctg gttgggaaaa cgaagttggt taccctgtag
    16261 aagaattttt tcaagattac tttgcggagt atgccgatag gataatgaga gattacgtaa
    16321 cagataaaac tcaagaagtc atcaatcaat cccagtcatt aaatgaatta tctgataagg
    16381 cgaaagaaga agcagttgcc attatttctt cagggattag tgactatatt gatatgagtg
    16441 gtgaaatcaa ttatggcgat aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa
    16501 cttttgatgt gaaggcagat cttaagttta atgatactta cctagtagga aaattaatta
    16561 atatccttgg gacagagaaa gatacggaaa cgtttatttc ctctttgtct atgttgttta
    16621 cagataagca tttttcaact cagcttggtg gagaggaact agtggaaatc ttttaaccat
    16681 tacgcccagc attaataaaa gtatatcaaa aaagtactcc tgatgatttt taagattggt
    16741 ttgagaaata atgtgaaaac actattttta atgttaatta tagatagaga tgaacgcgtc
    16801 atatggttaa aagaatgaaa aagtcaaaga tacactgcta tatttcacta aggatatagc
    16861 agtgtatctt tcatgtcgtt tcactggcag ttttttataa aaggaggtgt gcatttcagt
    16921 tgcttttctt tggtagttag ttgaatcgtt taagaattaa cggatgtggc agacaggtgc
    16981 tctgtccaaa aaccagtaaa atctttgatg gttccatctt tatttagctg tgcctgttca
    17041 aatactgttt tgtttttacc gaatggatca attgcaataa ttgttccgat ccccgcacgt
    17101 ttggcagcaa gtaatcctga gtaagcatct tcgataacaa gacaatcagc cggagctaag
    17161 ctcagtttgt ctgccgcttt aagaaaaata tccggttgcg gtttcccagg gaaagagccg
    17221 tcgtcataga caatcttgtc ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa
    17281 aaagcaacat tctctttgac agtagccgtg gcaatcgtta atggaatctg ttggtcagtc
    17341 agctggtcaa gagtcgcttc aagccctttt gtcaactgaa gctcgttttc attttgcaag
    17401 caaagcatac gataatagct ttctttttct tctgacaata cggcgatctc agaggcggtc
    17461 aaatgctctg aaatgaaatg tttcaaaatc tccgtattcg tccgaccatg aatatttttt
    17521 aaaatatcct cgtctgataa accatctttt gtatgatcat gtatcatatg cagccaagca
    17581 gcttcatgca gatgagagtc caaaaacatt gttccgttga aatcaaatat aatccctttc
    17641 atagttacgc tcctatagtg gtattttcgg ttgaatcagc aatttgcaga tcgacatatt
    17701 gtgcatagtg ctcttgaacc tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat
    17761 catgcaaatt ataaaacaca taaaaatcat ctcgattgaa ttttttatta tcaacgagta
    17821 gatatttctt acgggaatta tttagagcga tctgctgtgc ttctccttct tcggtactgt
    17881 aagtagagat cgcttcgttg tgaacgccat ttgaactgat aaatgctttt gtaaacttta
    17941 gtcttcttaa attgtcgttg gtgatcggtc cgacaaatgc gccggtattt ttccggaaat
    18001 caccgccaat cagtagaatt tctggtccat ctgcttgagc aagaatatca aaaactggat
    18061 aattatttgt aatgatccga attttcttgt tcagtaaatg cgtcgccaat agttcgattg
    18121 ttgtacctgg tcctaagaaa atcgtttctc cctcgtgaat caaggaggct gctgtggcag
    18181 caatttcttt tttttcttga atctgcagag tggctttttc agtgtgagaa agttcttgat
    18241 ctaaagtgta ggaaagactt tgagcccctc catggacacg cagcagcttg cccgattttt
    18301 ctaattcatc taaatctctg cgaacggtca tatcagaaac atctaattga ttgatgattt
    18361 cattaacggt aaggattcct tttttattga ccatttctac gatagtaagc aatctttctt
    18421 ttttcaacat tgtcttcacc tctaatttaa ggataatcta tcatataaac ggacaacagt
    18481 caatctaatt cgaacaaaag taaacattta gggtggtttt tatgtttgtt acaatagatt
    18541 ttattgctgt tttctggttt ttatgattat ttgtgtttgt ttttgttgac aaatgttttg
    18601 gatgcgttta caatgcaatc agttagaaag agcgaaaact gattatagat ttgaggagag
    18661 atgtacatgc ttaaattacc agaagatttt atttttggcg gagccacagc agcctatcag
    18721 gttgaaggag ccactaagga aggtggcaaa ggcgctgttg cttgggatga ctttttagag
    18781 gaacaagggc gattcagtcc cgatccggca agtgattttt atcatcaata tgcaaaggac
    18841 attgaattgt gtgaacgttt tggtgtaaat ggtctaagat tatccattgc ttggagccgc
    18901 atttttcctg atggagctgg gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg
    18961 ttcgaagaat gtaagaaaag aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc
    19021 cctaaacgtt tgtttgacca tggagatttt ttgaatcgag aaacaataga agcatttgtt
    19081 tcctatgcca ttttttgttt tcatgaattc aaagaagtca aggtgtggag tacctttaat
    19141 gaaatctatc cagttgcaac caatcaatat ttgttaggtg ttttcccacc aggaatcaaa
    19201 tatgatttta caaagatcgt cgcctgtctt cataatatga tggttgctca tgcccgtgta
    19261 gtgaactatt tcaaagaaaa cgaacttccg ggtgaaattg gtgtagttca ctcattagag
    19321 acgaaatatg cggcaacaga cgcgccggaa gataagcatg ctgccttttt agacgatgca
    19381 ttgtcgatcc gtttcttatt agatgctacc tatttaggct attattcaac tgaaaccttg
    19441 actgctcttg atgagatatg tgaagcaaat caggccagct atcattttcc tgaagaagat
    19501 ttcgttgaat tgaaaaaagc atccacgcgt aatgactact tagggatcaa tcactaccaa
    19561 tgccattttg tcaaagctta cgatggagaa aatgcaatcc atcataacgg tacgggtgaa
    19621 aaagggacct ctgtttataa agtgaaggga atcggagaac gtatttataa agaaggaatt
    19681 cctcgaactg attgggactg gctgatttat ccagaaggtc tatatgactt gttgctgcga
    19741 attaaaagcg attacccaca ttacaacaaa atctatatca ctgagaacgg gatggggtat
    19801 aaagatcagt ttgaagatgg cattattatg gatcagccga gaatcgacta tttgagagtc
    19861 tatttggaat ccttaagcaa ggcgataaca gccggggtaa atgtgaaggg gtattttttg
    19921 tggtcgctta tggatctttt ctcatggaca aatgggtata acaaaaggta tggcttgttt
    19981 tatgttgatt ttgaaaccca gaagcgttac ccaaaagaat cagcttactg gtacaagttg
    20041 gtcagtgaaa ccaaaacgat tatttaataa gtgataatgg aggaagaaaa gatgaataaa
    20101 gaggaattac aaatgcttgg ttttgagatc gttgcatatt caggcgacgc tcgcagtaca
    20161 ttattgaaat tattgaaaga agtaagacaa ggaaactttg aacatgttga ttcggcgttg
    20221 aaagaagccg acgaaaattt aacccttgca cataattcgc aaacaaaaat tttagcggaa
    20281 gaagcatctg ggaaagagat ggagatggga ttcattttta tccatgggca ggatcattta
    20341 atgaccactt tattgctgag agatctgatt caagacttta ttgtactgta cagacagaat
    20401 caaggatagg agaagaacgc tatggatgga atcattaaac aaatcgaaaa agggaaacca
    20461 ttttttgaaa aaatctcacg taatagttat ttaggagccg ttcgtgacgg atttttaaca
    20521 gcgatgccag ctattttatt ttcaagcatt ttcattttga ttgctgcggt tcctgagatt
    20581 tttggttacc aatggcctga atctgtctcc acatggctat ggaaagtcta taattattcg
    20641 atgggtgttg tagggttact cgtttcagca accactgctc gctgcttggc tgattcgatg
    20701 aatcgtcgct taccgaaaaa caaagtaatc aacaccacct ctgtgatgct ggcctctgtg
    20761 gtaagtttcc tgttgttaag cgtctctccg attgaaggtg gattttcaac ggaatatatg
    20821 gggacaaagg gactactggc ttcctttgtc gctgcatttt taacagttaa tttatataag
    20881 ttttgtgtgc tgaaagatat taccattaaa atgcctaaag aagttcctgg aacgatttcg
    20941 caaaccttcc gagatatttt tcctttctct ttttcagtat ttgcggccgt tttaattgat
    21001 ttaggcattc gacaaatctt ccaaatctca tttgcagaag caatcattac ggtgctgcag
    21061 ccgctattta cagcagctga cgggtactta ggaatcgcta ttatctgggg agcgatggcg
    21121 ctgttttggt ttgtcggtgt tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt
    21181 atctatgcca atgtggaaac caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa
    21241 gtactgacgg tgggtttagg gaattttgta ggaactatgg gaggaactgg ggcaacattg
    21301 gttgttcctt ttatcttcct actctttgct aagtctaagc agttgaaggc agttgggaaa
    21361 gcttccttta ttccagtaag tttcgctgtc aacgaaccat tgctatttgc ggcacccatg
    21421 attttgaacc cttatttttt tattccattt ttgttaacac ctgttgtaaa cgtttgcctg
    21481 ttcaagtttt ttgtcgatgt attgggcatg aacagcttta tgtacgtttt gccttgggca
    21541 actccagcac cgatcggttt ggttctagga acgggcatgg gtatcttagc ttttgttcta
    21601 gcagcggttc taatcgtcgt tgacttcctg atctatctac cattttgcaa agcatacgat
    21661 gctgtcttag tagagaaaga agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct
    21721 tctgccgatg tacgtgagga agtacctgtg gcagcgagtc aagttatcag cgatgcaaat
    21781 aaagaaatca atgtgttggt actttgtgct ggcgcaggga caagtgcgat gttagctaat
    21841 gctttaaaaa aaggtgcatc tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat
    21901 ggttctcatt acgaaatgct gcaggatttt gatatggtgg tcttagcacc gcaagtcaat
    21961 tcttactatg atgatatcaa agaagacacc gatcgcttag gaatcaagct ggttgcgaca
    22021 aaaggagcag aatatatcgg attaacgaga gatcctgaaa aagctgttca gtttattctg
    22081 aaccaatttc aataaaggag gagccataat gaaaatggga atttggttgc gtaaagctgt
    22141 tttatttgca gctgtgttag gggctttggg ggctgtttca aaaactgttg aagcatctgc
    22201 cagcgaattt attcgcggag cagatatttc gatcctgtca gatatggaaa aatctggtgc
    22261 aaaatattat gaagacgata tttcaaaaga tgccttagag attttaaaaa ataatggtgt
    22321 caactatgtt cgactgcgtt tgtggcagga tccttataat agtaatggag aaagttatgg
    22381 agcagggaca aatgatctgg aaacgacgat tgaccttgca aaacgagcaa aaaatctggg
    22441 acttaaggtg ctgctggatt ttcactacag tgatttttgg gttgatcctg ggaaacagaa
    22501 tcttcctaaa gcatggcaag gtctgacctt tgaagaaatg aatacggcat tgtacgacta
    22561 tacaaagaac gttctgtcgg aaatgaagca gctggacgtt tatcctgata tggttcagat
    22621 cggcaatgaa ctgaatagcg gaatgctctg gccttatggc aagtcttggg gagaaggcgg
    22681 gggagaattt gatcgattgg ctgctttttt gaagtctggt atccaagctg ttcgagacac
    22741 tcaacctaaa acaactccag taatgctcca cttagctgat ggtggcgata caggagcatt
    22801 tacttggtgg tttgatgaaa tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc
    22861 gtattatccg tattggcacg gttcaatgaa tgaacttcaa accaatatga atgcgatcag
    22921 ccaacggtat ggtaaagaag tgatcgtagt tgaaacagct tatgggcata caacagcgaa
    22981 tgccgatact atgccaaatg cttttggtga agccgaagca gctgcggggg gatatgaacc
    23041 aacaccgaca ggacaagcag agtatttgct ggatcttgcc gatcggattc aggcagtacc
    23101 gaataatcgc ggggcgggct tcttctattg ggaacctctt tggtacaacg gaaatgtgag
    23161 ttgggcaaca caagcaggta tgaattatct cagtgttcaa tccactatgg gaaacgaatg
    23221 ggacaaccaa gcgatgtttg attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa
    23281 acaagcaggg gcgcaaataa atctagttaa aaatgcaagt ttcgaaagcg atggaccaac
    23341 gaccagtcct tccaattggc aaacctggtt tcaacaagga accgctaagg gtacgatcaa
    23401 aacggaagcg ggcacttttt ttggggacta caaactaact ttttggcagg atcaagcata
    23461 cgaggcatca gcttatcaaa atgttagtgg cataagcaaa ggaacttaca cattgagtgc
    23521 ctatgtaatg tctgggggag agtttgatac taatcagtta tatatcaaaa attatggtgg
    23581 agcggaacta aatactgcgg ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga
    23641 cattccaatt acaacaggtg tttgtgaaat tggggtttat agcaaggctt caggaaatgc
    23701 ttggacgaac gttgattttg tttcattggt gaaacaatag actgcggatc gtttcgattc
    23761 aagatcggag gttcccttca gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac
    23821 ctcgcctttg ccaaaaataa ccagaaacga tttccagtca tttttaccaa aacgagtaaa
    23881 gcaccagact tcatctacac tttgtattat gctaaagaga gttcaacgat ttcgtaagaa
    23941 gcaatgaaaa aattgcaaaa agttgtgcag ataacataaa gtttgtttaa gaaagggtaa
    24001 ccttagaatt ccgtcgtgaa tatgatttga gcacttagat ggatagaaaa aagttctcgg
    24061 aaaacagttc gtttttcgag aacttctttt tatgataaga atgaaacaaa ttaacttata
    24121 ttatagtctc ttttggtagg gaaccatctt atttttaatg aaaatcaagc attttagcta
    24181 ttgaagaatg ttgatttata gggattcccc ctccatatta tgttggctct ttattcccac
    24241 tccactgttg ctggtggttt agaagtgatg tcatacacga tacggttgac gtgggctact
    24301 tcgtttacga tacgtacaga gattttttgc aagacttccc atgggatacg ggcaaagtca
    24361 gcggtcattc cgtcgattga agttacagca cggataccga ctgtgtagtc gtaagtacgt
    24421 ccgtcaccca taacaccgac agagcggatc cctggaagaa cagtgaaata ttgccagata
    24481 tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg
    24541 atttctagct tgtcttcagt gatttcgcca agaacacgaa taccaagacc aggaccaggg
    24601 aatggctgac gccaaacgat tgaatcaggc atgcctaact gagtccctaa ttcacggact
    24661 tcgtctttga ataatgtgtt taatggttcg atcaattcaa attgcatatc ttctggcagt
    24721 ccacctacat tatggtgaga tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg
    24781 tcagtataca acgttccttg tgctaggaaa gagacgcctt cttcaccagc aagttttgtt
    24841 gcttcgtcat caaaaacata tacgaattca ttcccaatga ttttacgttt ttgttctggg
    24901 tcagatacac ctttcaactt gtctaagaag cgatctcttg cattgacttt gatgatgttc
    24961 aagccgaatt ttccgcctag actttccatt acttgttccg cttcgccttt gcgtagcaga
    25021 ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca
    25081 acgacactag aatcaacgcc acctgaaaga ccaagcaaga cttttttgtc accaacttgt
    25141 tcacggattt tttctacttg catatcgata aagttatcca ttgtccagtt accttcacag
    25201 tgacaaacat caaaggcaaa gtggcgcagc aattcgattc cgtattcgga atgacgtact
    25261 tctgcatgga attggatacc ataaaaatta cgtgttgtgt cttggataga agcaatcgga
    25321 caatctttgc ttgttccgac tgtttcaaag ccttcaggaa ctttagttac caagtcacca
    25381 tggctcatcc atactgtttg ttgttttggt gtttctttaa acaagacagc ttgatcgctt
    25441 gttacttcta acaaggcttt cccatattcg cggtttttag caggctctac ggaaccgcca
    25501 agtttgtacg tgatcaattg cataccatag caaatcccta atacaggaat acctaaattg
    25561 aagatctcag gatcgatccc aaatgcattc tcatcatata cactgtttgg tccgccagaa
    25621 agaatgattc cctttggatt catcgctttt acttcttctg cagtaatgcg gtggctcaat
    25681 aattcggaaa agactccaag atcacgaata cgtcttgtga tcaattggtt atactgacta
    25741 ccgtagtcta atacgataat cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt
    25801 attaccccta tccctttaaa ttttatctca aaatagtcaa actattttaa aatgctaata
    25861 ttttcgcatg aaaatcgaat cgatgatatg attttcagtt ttatggagaa ttatatcggc
    25921 tcttcctcgt gtaggcaaga tatattcctg taaatttttc aaattcacat ttttccatac
    25981 atcttttgcc attttaaagg cttcttcacg atctccgata gcaaaacgat aataataatt
    26041 ctctggattt tggaaagccg tatccagtaa cgcaccaaaa cgatccaaat accatttttc
    26101 gatcagcacg ggatcggcat cgacatagac agaaaaatca aaaaagtcgc tgacgtagat
    26161 ttgctgattc gcaggtaatt gaagcgtatt gatcccctca acgatcaaga tatcaggctg
    26221 ctggatcaat tcgtacttgt ctggaatgat atcatacaca tcatgtgagt aaacaggtac
    26281 ttttatctct tcctgaccag attttacacg gttcaagaaa tcgatcagca tctccatatc
    26341 ataactttca ggaaatcctt tacggtccat gattccacgc tcttttaata tacgatttgg
    26401 ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt cgtttgaagg tacgagacaa
    26461 aatcgtctgc agcagtctgg ctgttgtact ttttccaacg gctacacttc cggcgatccc
    26521 gataataaaa ggcggtactt ctacgtattt atgaagaaat aaacctttac tcaagaacaa
    26581 agattcaaat tctttcatat aaagataaat cagatgagca agaggtacat agatatctcg
    26641 aacatcctgc atggaaatgc ggtcattcaa acttttgata ttatctagtt catcttgtgt
    26701 caaaggtgct tgcccattac gataaaattc tcgccattct tctcttgcca cacggtagta
    26761 gttcattgga tcattcatat tagcctcctg cgttctccca tcaatggaac tattttaaca
    26821 caaaataaac gcaaacccca ccacttaaga gagattgccg atctttttgt ataatcttta
    26881 taatagaaag aaacggagtc ttattcttga cttgcgcacg gatacataaa aggatagact
    26941 gttgtcatga aagaaatgag aaggatggaa gacatgacaa atcattacta ttctgaacgc
    27001 ccagatacgg cgcacgattt tgaacaatgg gcttttgagt taaaaggcag aacctttcaa
    27061 tttgtaacag attctggtgt attttcacgt gaaacagtgg attatggttc acgtgtcctg
    27121 attgatgctt tcgattggga agagttacct gatgaaggaa agatcttaga tgtgggatgc
    27181 gggtatggac cgattgggtt agctttggca tttgcgacac agcggtttgt tgaaatggtc
    27241 gacattaatg aacgagcggt aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa
    27301 catgttgata tccatcaatc gaatatctat gaagcagttc atgaagaaac ctatgcagct
    27361 atcgtaagca atccgccgat ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc
    27421 gcatatcctc ggttgaagaa gggcggtaca ctgacaattg tgatccagaa gaaacaagga
    27481 gcgccgagtg cgcaaaagaa aatggaagaa acatttggta atgcagaaat cgtcacaaaa
    27541 gataaaggat attatatcat caaaagtgtg aaagaataac tagctgaaaa atagtggagc
    27601 gacgaaagag tggaactggg cgtgcatgta cgggtacgta tcagttcttt ttttgttgag
    27661 aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga aatagtagga acagagtcgt
    27721 tgttttttac gataaaataa ataagataca gaacttgtat agaaggctgc caagtcggta
    27781 gataaaggag gtttttatca aaaaacatac tttccttttt tctctattct tttcttgggg
    27841 gttttggtac actgataggg aatttagata gaagggagcc aataggatga agctgaccat
    27901 cagagatatc gcagaaatgg caggcgtatc agtaacaact gtctcacaga ttttgaataa
    27961 caaaggaagc agatttagtg ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca
    28021 tcattataag cccgattatt tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg
    28081 aatgatcgtc cctgatgtca ctgatttctt cttctccaaa ctgatcgaag gtgtagagag
    28141 ctatttgaat cctttgggct atgtgatcat gttatgcaat tctcgtcata gccaagaaaa
    28201 tgagatcaag tatttacaag agctttccca ccgctcagtg gacggaatct tgctggccac
    28261 accaaatatt ttacctgaga aatatgcatt agacagcggt ttttatcaaa aaatgccaat
    28321 cattttaatc gatcgcggat tgaatcgtcg ggataacgga cggcttatcg taaaggaata
    28381 tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat ggccatacga agatcggcat
    28441 gctgaaggaa accactggat actatcaatt agaagaacgt ttcaacggat atcgtcatgc
    28501 gctgaaagat catggcctgc cttttaatgg gaaatttgtt gaacatggag agttgactgt
    28561 ccaaggtgga tatactgcat caaaaaaatt actcaaacat aaagatatca cggctatttt
    28621 ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa
    28681 aatcccagaa gatatctcag tcataggatt cgatggatta aagctgtcag aatacatgat
    28741 tccgcaattg acgacagtgc agcagccaag ttttgatatt ggattttatg ctgcacgttt
    28801 cctgatcgat acgatcgaat ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa
    28861 attaattatt cgtgagagcg taaaagtatt gacaaagggc taactcggcg gtatattgct
    28921 tatgtaagca tttacaaagg ctagttagtg ctttttttat ttttagtaaa ccagtttact
    28981 aaagtggttt acctttccta aaaataatgt ataataccga atgaagacaa ggaggatcag
    29041 gcaatgcgaa tggtggatct gattgaaaaa aagagggatg gaaacgagct gtcgaaagag
    29101 gaaatcgagt acatcgtcac gaactatacg aatggaaaga ttccagatta tcaagtcagt
    29161 gcattattga tggcaatttt ttatcaagac atgacaaatg aagaaatcac gaacttaaca
    29221 ttggcgattg ctaattctgg tgatgtcatc gatttgagtt ctcttgaagg aataaaggta
    29281 gataagcatt cgacaggtgg cgttggtgac acgaccacat tgattttagc ccccctagta
    29341 gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg gtctaggtta cacaggtggt
    29401 acactagaca agctagaagc aattcccgga tttcagatcg agctttcgga tgaagaattt
    29461 gtacgcattg ttaatgaaag taaagtagcg gtaattggtc aatcaggaaa cttggcaccg
    29521 gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga ccgttgattc tcttccgctg
    29581 attgccagtt cgattatgag taaaaaaatc gcggcaggag cagatgcaat cgttttggat
    29641 gtgaccactg gagacggtgc tttcatgaag aacatcgaag atgcaagacg tctggcaaag
    29701 accatgacga gcattgggaa actggctaac cgagaaacag tggcggttat ctcagatatg
    29761 tcagaacctc ttggcgaagc tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca
    29821 ttacaaggaa atggtccaga agatctagta gaaatgtgct acgccttagg aagccaaatg
    29881 gttgttcttg ctggcaaagc taagacaacc gatgaagcaa gaacgttgct tcaagaagct
    29941 ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga tccaaaatca agggggagac
    30001 ccagcgatcg tggaacatcc agaacgaatt ttgactgcac gctacacaat ggagcttcct
    30061 gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg
    30121 atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg atcacgctgt tggattgaaa
    30181 ctgcataaaa aaattggtga cactgtgaca aaaggtgagt cactattgac gatctatagt
    30241 aacgacgaag aaatcacttc tgtcattgaa ttattatata aaaatattga aattggtgaa
    30301 tcggcaatga agccaacgtt gattcatgac atcatcacag aataaaggaa aaaagcggac
    30361 tttaccgttt taaagaaaag gggaaaagaa atggaactaa accgaatgat cgaccatact
    30421 attttgaaag cagacgcatc taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa
    30481 taccattttt attctgtctg catcaatcca gcatgggttg cgctagcgaa agaacaatta
    30541 caaggtgagc cagtagccgt atgtacagta atcgggtttc ctctaggagc aaacacaccc
    30601 gagacaaaag catttgaaac aacagatgcc atcaataacg gtgcagatga agtcgatatg
    30661 gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa
    30721 gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag ttattattga aacatcgtta
    30781 ttagatagag aagaaatcat ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt
    30841 gtcaaaacat caactggttt ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg
    30901 cgtgaaactg taggaccaga aatgggtgtc aaagcttccg gcggtatcca taacgaagaa
    30961 gaagcaatgg caatgatcga agctggtgct acaagaatcg gaacaagcgc aggagtagca
    31021 atcgtatctg gttcaactgg tgaaggctat taattttttc aaaggagaag ctcatggcag
    31081 aactcaaaca agaatggatc aacacagcta ttgaagcgtt agataaagcc tatgttcctt
    31141 attcccattt tccggtaggc gcttgtctcg taacggaatc aggcaagatt tatcaaggca
    31201 tcaacattga gaatgcatca tttggattaa cgaattgtgc cgagcgcaca gcttttttca
    31261 aagcagtatc tgaaggagaa aaaacattta cgcatctagt cgtagcgggg cacacccccg
    31321 atcctatctc tccttgtggt gcatgtcgac aagtaatggc agaattctgt tcccctgata
    31381 tgccggttac gctagtagga gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc
    31441 tgccttatgc atttactgaa aaagatctat aactcatgtt cattcagtca gctgactgtt
    31501 gaaaaataaa acaattattt taggaggctt tacagaaatg aaaaaagcaa aattatttgg
    31561 tttaggtgct gtcgcactag cagcagggtt atttttaggt gcatgcggca acaacggctc
    31621 tactgattca tccggtggca aaagctcatc agacacaaca actgcagcat tgatcacaga
    31681 cacaggtggt gtagacgacc gttcattcaa ccagtcagct tgggaaggac tagaaaaatg
    31741 ggggaaagat caaggacttt cacgaggaaa tgatggcttc caatatttcc aatcttcaaa
    31801 tgaatcagat tacatcccaa atattgacca agcattgaat gctggattca aaacgatttt
    31861 tggtattggt tataaactaa aaccagcgat ccaagaacaa gcaacaaaca atacaggtac
    31921 gaactttgtt attatcgatg atgtcatcga tggattagat aacgttgtat ctgcaacttt
    31981 caaagacaac gaagcttctt atcttgcagg tgttgcagca gcttatacga ctgaaacaaa
    32041 cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt
    32101 caaagccggt gttgatgcag gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca
    32161 atatgctggt gacttctctg ctccagataa aggacgttca attgctcaag gtatgtatgc
    32221 acaaaacgca gatatcatct tccatgcttc aggcggtaca ggtaacggtg tattccaaga
    32281 agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga
    32341 ccaatcagac gaaggcgaat acacattaaa cggcgaaaag aaaaacttca cattgacatc
    32401 tacactgaaa gcagttggaa cagtagtaga agatttggca caaaaatcag cggacggtaa
    32461 attccctggc ggcgaacaca ctgtttatgg attgaaagaa gacggtgttg gtctaacaga
    32521 aggacaactt tctgacgaag cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc
    32581 aggtgacgta aaagttccag aaactcctga agaaaactaa aaccatttct atagccttgt
    32641 tagacacatg tttgacaagg ctatggtttg attagaacaa gaattgttca tttatccaag
    32701 ctgccttgtg acagtttgga taagggaaca actccctgaa gtaatgtttg tttcaaaaga
    32761 agaacgctca ctaagcgtga atcttagtca atcgttgccc tagatagaaa tcgttaggat
    32821 gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat gcagaatatt accaaagaat
    32881 ttggaacatt caaggcaaat gacaacatca atttacaagt aaaggcagga gaaattcatg
    32941 cgttgcttgg agaaaacggt gctggcaaat ctacattgat gaacgtgctt tccggattat
    33001 tagagccgac atcagggaaa attttgatgc gtgggaaaga agtaaagatc acaagtccga
    33061 ccaaagccaa ccaattaggg attgggatgg tccatcagca ctttatgctt gttgatgcct
    33121 ttactgtaac agaaaacatc gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc
    33181 ataaaaaagc acgaaaagag atccaaaaag tttctgaaca gtatggattg tcagtcaacc
    33241 cagatgctta cgttcgtgat atttcagttg gaatggaaca acgggtagaa attttaaaaa
    33301 cactttaccg aggagcagat gtactgattt ttgatgagcc gacagctgta ttgacccctc
    33361 aagaaattga tgaattaatc gtgatcatga aggaattagt caaagaaggc aagtcgatca
    33421 ttttgattac gcataagtta gatgaaatca aagcagtagc tgaccgctgt acagttatcc
    33481 gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt aacctcacaa caattagccg
    33541 atatgatggt tggaagaacg gtttcattca aaacgatgaa aaaagaagcg aagcctcaag
    33601 aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga
    33661 aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg tatcgctgga atcgatggaa
    33721 acgggcagtc ggagttgatc caagctttga ctggtttgcg aaaggcagaa agcggacata
    33781 tcaagctaaa aggggaagac atcactaata aaaaaccccg aaaaatcact gaacatggtg
    33841 taggacacgt gccagaagac cgtcataaat acgggttagt cctagatatg acattatctg
    33901 aaaatatcgc actacaaacc tatcatcaaa aaccttacag taaaaacggc atgctgaatt
    33961 attcagtaat gaatgaacat gccagagaat tgatcgaaga atatgatgtt cgaacaacga
    34021 atgaacttgt tcccgcaaaa gctttatcag gcggaaatca gcaaaaagca attatcgctc
    34081 gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa tccaactcgt ggactggatg
    34141 taggtgcgat cgaatttatt cataaacgtc tgatcgaaca aagggacaaa tacaaagcag
    34201 tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta
    34261 tccatgaagg agaaattgtc ggtatcgttg atccgaaaga aacatctgaa aatgaattag
    34321 gtctattgat ggcgggctat accttagaag aagcacgaaa agagttagaa aagggggctg
    34381 gcgcacatga atgatcgcag cgaaaaattc cgtaatttat tagtgcctgt actttcagtt
    34441 gttttaggat tgatcttagg cgcgatattg atggctgctt ttggttttca gcccatccaa
    34501 ggttattctt ctatgctaga tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta
    34561 aggcaagcaa cgccgttgat ttttacagca ttaggttttt ctgtagctaa ctcagctggg
    34621 tttttcaata ttgggctttc tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg
    34681 gcacttgcgt ttccagatct gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt
    34741 gccttagctg gtgcgatggc agcggctgtt ccaggtttac ttcgagcatt ttttggaaca
    34801 agtgaagtga tcgtaacaat catgatgaac tatatcttat tatacgtaag cacatttgct
    34861 ttacatgaag tgatgcctga aagctatcgc tcaagtttgg actcttccaa tatgatcagt
    34921 caaaatgcct cactgcggtt accatggtta acacaagctt ttggcggctc tcgcgtaaac
    34981 tcaggattat ttttagcaat catcgcgctt attttcgttt ggctgctgat gaagaaaacg
    35041 actttaggat ttgaaatccg ttctgttgga ttgaatcctt ttgcttctga gtatgcagga
    35101 atgagtagta aacgtacgat cgttttgtcg atggtcattt ctggcgcttt ggcagggctt
    35161 ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct ttgttcaaac gacttcgcta
    35221 tctattggat ttgacggaat ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt
    35281 cttttatcag cgatcttgtt cagtattttg aagatcggtg gacttggcat gcagacagga
    35341 gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt tgattatctt ctttgtcggg
    35401 atcaacttcc tgattcgttt cgtcatggct aaagtcttcc aagggaaaaa acaagaagag
    35461 atcgttgcga caatagaatc gaaaccaacg aatcaaaacc atgaaggggg cgaactataa
    35521 tgtcgactat cgaattaatt gccttgatcc ttacttctac actggtttat tcaacaccgc
    35581 taattctaac gtcactaggc ggaacttttt ctgaacgaag cgggatcgta aacgtagggc
    35641 tagagggaat catggtcatg ggtgctttta gttccatcgt tttcaacttg acgatgagtc
    35701 catcacttgg ttctatgaca ccatggatcg ctattttagt gggtggcgtc gtaggtatcc
    35761 tattttctct gattcacgca gtagcaacag tcagtttgcg tgctgaccat attatcagcg
    35821 ggacagtaat taatctgatg gcacctgctt taggtgtttt cttgatcaaa gcatggtatg
    35881 gtaaaggtca aactgataac atcacgcaga atctggggta tttttctttc ccaggattag
    35941 cagatattcc ggtcattgga ccaattttct ttaaaaatac attcttacct gcttattgtg
    36001 ctattttgat tgctgtccta gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc
    36061 tccgttcagt tggtgaaaat cctcaagctg ccgacacatt aggtatcaat gtatatgcta
    36121 tgcgttattc aggcgtactg atttctggct tgttaggcgg tatgggcggt gctgtttttg
    36181 ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt tggacaaggg ttcatctcca
    36241 tggctgctat gatctttggt aagtggaatc ctttaggggc aatgggtgcc tcattgttct
    36301 ttggttttgc tcaaagcttg agtatcattg gcgcacaatt accagttatt tcttcgattc
    36361 cgccagtatt gctgcaaatt gctccatatt tgctgacaat cattgtgctg gttgttttct
    36421 tagggaaagc ttctggtcca aaagcaaacg ggaagaacta tatcaaatca aaataatctt
    36481 ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg tctcgccctt tttgttttaa
    36541 aggcaataaa aataaaaaga aagaaggaat aatcatgttt aaacgcgtac atctgatcgt
    36601 catggattca gtaggaattg gagaagcacc agatgccgat aagttcggtg atgttggttc
    36661 tgatacatta ggacacattg cgaaagaagc aggattgaac gttccacatt tagagcagct
    36721 tggtttagga acgatccgtc ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta
    36781 tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg atgacaggcc actgggaaat
    36841 catgggattg aatatcaaaa ctccatttcg tgtgtttcct aatggattcc ctgatgatct
    36901 gatcaaacaa atcgaagaat tttctggccg taaagtcgta tgtaacaaac cttatagcgg
    36961 aacagctgtt attgacgatt acggtgaaca ccaaatgaaa acaggcgatt taattgtata
    37021 tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga
    37081 attatatcgg atttgccaat tcgttcgtga tataacaaaa gatgatccgt atatgattgg
    37141 ccgaatcatt gcacgtccgt acgtgggtga accaggagac ttcacacgaa caagcaatcg
    37201 ccatgattat gcgttgaatc cattcggaca cacagtcctt gattctttga aagaagcagg
    37261 aaaggacgtg attgctgttg gaaagatcaa cgatattttc aacggacaag gaatcactga
    37321 atctgtccgc acgaaaagca atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa
    37381 cgatttttca ggaatcagct ttaccaattt agtagatttt gatgcattat acggtcatcg
    37441 tcgggatgta gtcggctatg cgcatgcgat tgaagaattt gacttacgaa ttccagaatt
    37501 gcttgaagcg atggaagaag acgacttgct aatgattaca gccgatcacg gaaacgatcc
    37561 gacgtttact ggtaccgatc atacgcgaga atatgtaccg ttattggttt acagtaaaaa
    37621 aatgaaagaa caaggggcat tgcctcaagg atattattca gatatttcag caacgattgc
    37681 tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata
    37741 atctttttaa ataggagaaa gcaaatgacc aagttaagcg agatgttaca agaaacgacc
    37801 cagtttatcc ttgataaagg agtaaaagaa attgattttg gtttgattct aggttcagga
    37861 ttaggtgaat tagcagatga aatcgaagat gctgtaatga tttcttatga tgagatcccg
    37921 ttctttcctg tttcgacagt agttgggcat gcaggaaagc tagtttatgg gtctctggca
    37981 ggcaagaaag tgttagccat gcaagggaga ttccactact atgaaggtca ttccatgcaa
    38041 acagtgacct atccagtacg tgtgatggcc ggactagggg cccattcagt cattgtgact
    38101 aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag atttgatgct gattaccgat
    38161 catatcaatt ttactggtga aaatcctttg atcggtccga atgaagatgc aatgggccct
    38221 agatttccag atatgagtca agcttatacg cctgactatc aagcaacagc aaaagcagca
    38281 gcagaaaaac taggtttgga tcttaaagaa ggcgtatata tgggctattc tggaccaact
    38341 tatgaaacgc ctgcggagat ccgaatgtct cggacaatag gagccgatgc agtagggatg
    38401 tcgactgttc cagaagtcat cgtagcagct cacagcggat taaaagtgtt aggaatctct
    38461 tgtattacca acttagcagc aggtatgcaa gcgaatctga gccatgaaga agttgtagaa
    38521 acaactcaga gagtcaaaca atcttttaaa gcattggtta aagaaacatt gacattattg
    38581 taataggagg aacaataaaa tgagtgtgca tattgaagcg aaagaaggcg agatcgccga
    38641 taagatttta cttccgggag atcctttgcg agctaaatat attgcggaga cttttttgga
    38701 agatccagtt tgttacaatc aggtacgcgg tatgttaggc tttaccggca aatataaagg
    38761 agaacgtatt tctgttcaag gaacaggaat gggaatgcca tcagccacca tctatgccca
    38821 tgaattgatc cagtcgtatg gtgtaaagaa attgattcga gtaggtacat gcggtgcatt
    38881 gtcaaaagac gtccatgttc gtgatttagt actagcacaa ggtgcagcga ccagttcttc
    38941 gatgatcgaa aaaaatttcc aagcctttca ttttcctcca atcagtgatt tcaatctatt
    39001 actgaaagca tatgagatag ctaaagaaaa gggctatacg gttcatgtag gaaatgttct
    39061 ttcggaagat tctttctaca aagatgattt gacagagacg ttccaattag ctgaacttgg
    39121 cgttttagga gtggaaatgg aagcagcggc tctttattac ttaggcgcga aataccatgt
    39181 gcagacatta agtttgatga ccgttagcga ccatttgatc accggtgaag aaacaacagc
    39241 tgcagaacgt caatctacgt tcaatgaaat gatcgaagta ggattggaaa cagcgatagc
    39301 agaataagaa ggaaatgaaa aagctgccct gcattaagta ataagaacat ccaagcaaaa
    39361 atagctcctc atgtttttga cttggctgga cttattattg caaatgtaag cttttgaaca
    39421 ccgtttacca agaggttgtg aatctgccgt ttaactctga gtcacaagga acaatttcaa
    39481 aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgagaagtt ggcaggagaa
    39541 aaccgtttat acgaaaaaag ggactgtgac aaatcgtgtc acagtccctt ttttatagtg
    39601 ttttttatag gtagtatttt ttagcaactg ttaagtcatc atttaattca taaacaagtg
    39661 gttgacctgt tggaatttca agatccatga tgtcttcgtc agagatacct tcgatatgtt
    39721 tagctagggc gcgtaatgag ttaccatgtg cagctactaa aacagttttg ttgtctagta
    39781 atgctggagc aatttcatct tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt
    39841 tttctccacc aggaatatcg cgtttgtcta acattgcgta acgacggtca tttgctgcag
    39901 aaccttcgtc agtttcttcc attaatggag gaagagtatc gtaagaacga cgccagatgt
    39961 gcacttgttc gtcgccgtat ttttcagctg tttctttttt gttcaaacct tgtaatttac
    40021 catagtgacg ttcgtttaag cgccatgatt tgatttgtgg tacccaaagt tgatctgaat
    40081 attctaagat caagttacat gttttgattg cacgagttaa aacagaagtg taagcaacat
    40141 caaattcgat tccagcttct ttgattttgc gtccgccttc ttttgcttct tcgattcctt
    40201 ctggtgctag atttacatca gcccaaccag taaattggtt caatgcattc cattcactaa
    40261 gaccgtgacg agaaaaaact aattttggca tgtttagctc tccttttaag ataggtttat
    40321 atttctgaaa ttttcagttc tgttttattg tacaattttt tggaagagat ttccatcgaa
    40381 aaattagata agattctatg agaaaacaag tagagaatat aaaaaaacga acagtgccca
    40441 cgcaactgtt cgtttttagg tattagatgc ataattgctc gatttctttt tcaggtaaat
    40501 tgatatattt ataacatgtt ccaactgaaa caccgcattt tgaagaaata aattggatcg
    40561 tttctttttt ttcataatag agacgtcgga tttttttgat cgtttttgca tcgatttttg
    40621 gtcttccgcc gatttttcct tttttacgag cttcatctaa tccaactaat gtacgttctt
    40681 tgatcaatgc gcactccatg tttgctaggt tttccattaa ttggaaatag atttttccca
    40741 tttcgcgcgt atcgattcct tcatcaagac taatcaagtg cagcccgcga tctttgaata
    40801 attgcgtgag ttctgttaat tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga
    40861 ctagtgcatc gccttgttgc atgtcagcga tcactgactc caaatgaaca gcagtagaat
    40921 cattttgttc cacttcatat gtctcatgga aaatttcttg acaaccaaag tcagataatt
    40981 gatcaacctg agttgttaaa tcaacgtcat ttatggttgt gcgtgcataa ccaataattt
    41041 tcatggtgat tcctttctag cagtgatttt ctaactggat ataacagtaa aaatgtagca
    41101 taaagtacgt caaaaagata gttttttcgc aaattttccg ctgtcttttt ttattggatg
    41161 atcaagcagt ttttctttat ttacgacgga ttgcgtcaag aatctcaacg cctttttctg
    41221 agccgacaac tactttagaa accatgttta ggaaaagccc atgttctact accccaacaa
    41281 gttcatctaa ataggtagct aagctttctg gctgttcgat gacttctaga tgcagatcaa
    41341 tgatgtaatg accaccatct gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg
    41401 gttggaatct tttttcttca aaaatatgaa gtagctgttg ggagccataa gggatcactt
    41461 caactgggag tgggaacttc ccaagtttat cgactaattt agaagaatca acgatccaaa
    41521 tcgtttcttt tgagtacgta gcgactattt tctcgaaaag caaagcagcg ccgccgcctt
    41581 tgatcccttg aaaatcagca ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg
    41641 ggacttcatc aatactcttt aacggtattc ctaatttttc cgcttgttct tttgtcgcat
    41701 ttgaagtagt aactcctgtg atacgtaaac cttcttcacg catacggcga ccaatctctt
    41761 ctaccatata ataggcggtt gaaccggtcc cgagacctac gatcatcccg tcctttacga
    41821 atttcgcggc ttcaatgcca gctaattgtt ttaaattcat ctttgcgtcc accttttttt
    41881 agtttgagca tcgtgctttc ctcactattt tagcgtgatt tacggtaaaa agataccatt
    41941 cttttttatt ccaataaagc tttttcaaaa aaaattgcat ctcttgttga catgtttttt
    42001 cattcgtgat attctaacaa aggtgcttta tctacaggtt tctgtgaaga aacgtgctga
    42061 ctgtttatca aagcatattt gataaggatg cagacattgc atttattttt tatcacaaaa
    42121 aaagaaccac ctggatgtgt ggaactagac gcaaatcgtg ggaaggagga aacaaggaat
    42181 gcctacaatt aaccaattag tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc
    42241 acctgctttg aacaaaggat ataacagctt taagaaaact caaactaacg taaactcacc
    42301 tcaaaaacgt ggggtagcaa ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc
    42361 tttacgtaaa tatgcacgtg ttcgtttgtc aaacttaatt gaagttactg cttatatccc
    42421 agggattggt cataacttac aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa
    42481 agacttacca ggggtacgtt accatatcgt tcgcggtgct ttagatactg ctggtgttaa
    42541 cgaccgtaaa caaagccgtt ctaaatacgg aactaaaaaa cctaaagctt aattttaatt
    42601 attaaacaaa tgaaacttgc ttctactata tagaagtaat tactatgaac aatatttcgg
    42661 aaggaggagt tacggatgcc acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat
    42721 cctatttata actcaaaatt agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa
    42781 cgcgggattg cagctaacat tatctataat gcctttgact tgatcaaaga atctacaggt
    42841 aacgatccat tagaagtgtt tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt
    42901 aaagcacgtc gtgttggggg ttcaaactac caagtaccag ttgaagttcg ccctgaacgc
    42961 cgcgttactc taggtcttcg ttgggtagta aactacgctc gcttgcgcgg tgaacacaca
    43021 atggaagaac gcttagcaaa agaaatcatg gatgctgcaa acaacacagg tgcttctgtt
    43081 aaaaaacgtg aagacactca taaaatggct gatgccaacc gtgcgtttgc tcactatcgc
    43141 tggtaagatt ctctctgttt ggtcggcttt tataagtcga cctcagatag tgtccttatt
    43201 tttagcaact agatagtaaa gagaggagaa acaaggagaa tgaccagaga attttcttta
    43261 caaaacactc gtaatatcgg tatcatggcc cacatcgatg ctggtaaaac gactgctact
    43321 gaacgtatct tgtactatac tggtaaaatc cataaaattg gtgaaactca tgaaggagct
    43381 tcacaaatgg actggatgga acaagaacaa gaacgtggta ttacgatcac ttctgctgct
    43441 actacagcac aatggaaggg tcaccgaatc aacatcatcg acacaccagg acacgtagac
    43501 ttcacagttg aagttgaacg ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat
    43561 gcacaatcag gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac aacttatggt
    43621 gtacctcgta tcgtatttgc aaacaaaatg gacaagatcg gtgctgactt cttatattca
    43681 gtatcaactt tacatgaccg tttgcaagca aacgctcacc caatccaatt accaattggt
    43741 gcggaagacg acttcacagg aattatcgac cttgtaacaa tgaaggctga aatgtataca
    43801 aacgatcttg gaacagagat cgaagaaaca gaaattcctg aagaataccg cgaattagct
    43861 gaagaatggc gcgaaaaatt ggttgaagct gtagctgaaa ctgatgaaga acttactttg
    43921 aaatacttag aaggtgaaga aatcactgaa gctgagttaa aagaaggtat ccgccgcgca
    43981 acagttaacg tagaattcta cccagtacta tgtggttcag catttaagaa caaaggggtt
    44041 caattactat tggacgctgt tcttgattac ttgccatcac cgttagatat cccagctatc
    44101 aaagggattg accctaaaac tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct
    44161 ttctcagctt tagcatttaa agttatgact gacccattcg taggtcgttt aactttcttc
    44221 cgtgtgtact ctggtgtttt acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa
    44281 cgtgaacgtg ttggtcgtat cctacagatg catgccaact ctcgttcaga aatctctgaa
    44341 gtttacgctg gagatatcgc tgctgctgtt ggtttgaaag acacaactac tggggacaca
    44401 ctatgtgatg aaaagagtct agttattctt gaatccatgg aattcccaga accagttatc
    44461 caagtcgcta ttgaacctaa atcaaaagct gaccaagata aaatgggtgt ggctttacaa
    44521 aaactatctg aagaagatcc tactttccgt gctgaaacaa acgtggaaac aggcgaaaca
    44581 atcatcgctg gtatgggtga acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa
    44641 ttcaaagttg aagctaacgt aggtgcgcct caagtatctt accgtgaaac tttccgtgct
    44701 ggcactcaag ccgaaggtaa gtttgtacgt cagtctggtg gtaaaggtca atacggtcac
    44761 gtatggattg aatttacacc aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc
    44821 gtcggtgggg ttgttcctcg tgaatacatt ccagctgttg aaactggttt gaaagatgct
    44881 atggaaaatg gtgtcttagc tggctatcca ttagtagata tcaaagctaa actttacgat
    44941 ggttcatacc atgatgtcga ttcaaatgaa actgccttcc gtgttgctgc ttctatggca
    45001 ttacgtgcag cagctaagaa agctaatcca gtaatccttg aaccaatcat ggctgtagaa
    45061 gttgttatcc ctgaagatta tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga
    45121 cgtgttgaag gtatggaagc tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca
    45181 cttgctgaaa tgtttggtta tgctacaact cttcgttctg ctactcaagg tcgtggtaca
    45241 tttacaatga catttgacca ctacgaagat gtaccaaaat cagtacaaga agaaatcatt
    45301 aagaaaaacg gcggaaaagc tgaataattt cttgaaaccg gatagtcctg caaagagatt
    45361 atccaaagag actatatttt taatatagtt tcgtgtaaaa taattatcga tgatatgggc
    45421 atgtggtgta ccttgtgtac atcatatacc tatcagacaa ataaaaaata tattgtattt
    45481 ttaggaggac attttaaaat ggcaaaagaa aaatttgacc gttctaaacc acacgttaac
    45541 attggtacta tcggacacgt tgaccatggt aaaactacat taacagctgc aatcacaact
    45601 gtactatcta agaaaaacgg cggccaagct atggcttacg atcaaatcga tggtgctcca
    45661 gaagaacgcg aacgcggaat cacaatctca acagctcacg ttgagtatga aacagataca
    45721 cgccactacg ctcacgttga ctgcccagga cacgcggatt acgttaaaaa catgatcact
    45781 ggtgctgctc aaatggacgg agctatcttg gtagtttctg ctgctgacgg cccaatgcct
    45841 caaactcgtg aacacatcct attgtctcgt caagttggtg ttccttacat cgttgtattc
    45901 ttgaacaaag tagacatggt tgatgacgaa gaattactag aattagttga aatggaagtt
    45961 cgtgacctat taacagaata cgaattccct ggtgacgatg ttcctgtagt tgctggatca
    46021 gctttgaaag ctctagaagg cgacgcttca tacgaagaaa aaattcttga attaatggct
    46081 gcagttgacg aatacatccc aactccagaa cgtgacaacg acaaaccatt catgatgcca
    46141 gttgaagacg tgttctcaat tactggacgt ggtactgttg ctacaggtcg tgttgaacgt
    46201 ggacaagttc gcgttggtga cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa
    46261 acaacagtta ctggtgttga aatgttccgt aaattgttag actacgctga agctggagac
    46321 aacattggtg ctttactacg tggtgttgca cgtgaagaca tccaacgtgg acaagtttta
    46381 gctaaaccag gtacaatcac acctcataca aaattctctg cagaagtata cgtgttgaca
    46441 aaagaagaag gtggacgtca tactccattc ttcactaact accgtccaca attctacttc
    46501 cgtacaactg acgtaacagg tgttgttgaa ttaccagaag gaactgaaat ggttatgcct
    46561 ggcgacaacg taacaatgga agttgaatta atccacccaa tcgctatcga aaacggtact
    46621 aaattctcaa tccgtgaagg cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa
    46681 gcttaattca cttaagtttt aaagtaaagc aacatttatt cggacgtaat cgctcggaac
    46741 gaaggtctta ggatctttgt tccgagcttt tttatttatt gcaggatcag gaaaaaagac
    46801 aggagtatta taaatagaag aaacacgaaa taaaagagtc agtattttct aaatgaaact
    46861 ttatgaaaac aaaatgtttt ttacaagaaa aagccttgct ttgtgagttt atatatatta
    46921 taatgacaaa ggtgcgttta ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc
    46981 ggctaagaaa cgagaggttg cgacacgccc ggacgctttg ccacgaacga gcgtgacgga
    47041 aattttcgtg gagctatgtc tacttttcaa aataggcgaa ggagggaaca agatggcaaa
    47101 acaaaaaatt cgtatccgtt taaaagcgta tgaacaccgt attttagatc aatcagcgga
    47161 taaaatcgtg gaaactgcaa aaagaactgg agctagcgtg tctggtccaa ttccattacc
    47221 aactgaacgc agtctttaca caatcatccg tgcgactcat aaatacaaag attcacgcga
    47281 acaattcgaa atgcgtacac acaaacgtct tatcgacatt gtgaacccaa caccaaaaac
    47341 tgttgatgct ttgatgaagc tagacttacc atctggtgta aacatcgaaa tcaaattata
    47401 aaactaaaac aaaaataatg gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt
    47461 gggaatgaca caaatcttca ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc
    47521 tactccaaac gtagtactac aagtgaaaac aatggaaacg gatggctacg aagctattca
    47581 agttggttac caagatatgc gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc
    47641 aaaagcaaac acggctccta agcgcttcat tcgtgaattc aaaaatgttg agctaggaga
    47701 atatgaagta ggaaaagaaa ttacagtaga tgttttccaa gcaggagaca ttattgatgt
    47761 aacaggtact acgaaaggta aaggattcca aggggttatc aaacgccacg gacaaagccg
    47821 cggaccaatg gctcacggtt ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc
    47881 acctaaccgt gtatttaaaa acaaacgtct tgctggccgt atgggtggca accgcgtaac
    47941 tatccaaaac ttggaagttg tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg
    48001 taatgttcct ggagctaaaa aatctttaat tactatcaaa tcagctgtga aagctaaata
    48061 attgtaggaa gaaaggagga actaaggaat gccgaatgta gcattattca aacaagatgg
    48121 aagccaaaac ggtgaaatca cacttaacga agaaatcttc ggaattgaac ctaatgaaag
    48181 tgttgtttac gatgcaatcg taatgcaacg tgcgtcatta agacaaggaa cacacgcggt
    48241 taaaaaccgt agcgctgttc gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg
    48301 tcgtgcacgt caaggatcta tccgctcacc acaatggcgt ggaggtggcg tagtctttgg
    48361 accaacacca cgttcttaca gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa
    48421 atctgtattg tcagaaaaag ttgctgaaaa caacttggta gcaatcgaag gcttgaactt
    48481 cgacgcacca aaaacaaaag aattcaaaca agttcttgct aacttatcta tcgactctaa
    48541 agtattggtt gttctagaaa aaggtaacga ctttgctgct ttatcagcac gtaacttacc
    48601 aaacgtttct gtagtaactt ctgataatgt gagtgtgcta gacgttgtat caaatacaaa
    48661 agtgttagca acacaaactg ctcttactca aattgaggag gtgcttgcat aatgaactta
    48721 ctagacgtaa tcaaacgccc agtgatcact gaaaaatcaa tgcttgccat ggatgacaag
    48781 aaatacactt ttgaagtgga cactcgcgca aacaaaactt tagtaaaaca agcggtagaa
    48841 gctgcttttg acgttaaagt caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa
    48901 cgtatgggca aatatgcagg atatacaaaa aaacgtcgca aagcgatcgt tacattgact
    48961 gaagattcaa aagaaatcca attattcgaa gctgctgaat aagcactaat agataactca
    49021 ttaaattagg agggaaaaac gtggcgatta aaaagtacaa acctacctca aatggtcgtc
    49081 gtaacatgac aggttctgat tttgctgaaa tcacaacatc aacacctgaa aaatcattgt
    49141 tacaaccatt gaaaaaccat gctggacgta acaacaacgg ccgcatcact gtacgtcatc
    49201 aaggtggcgg acacaaacgc caatatcgcg tgatcgactt caaacgtaac aaagataacg
    49261 ttgttgcaac tgttcaaacg atcgaatatg atccaaaccg ttcagctaac atcgcgttag
    49321 tacactatga agatggagtg aaagcttaca tcttagcacc taaaggacta gaagtgggta
    49381 tgaaacttgt ttcaggtcca gaagcagata tcaaagtcgg aaatgctctt ccattagaaa
    49441 acattccagt tggtactgtt atccacaaca ttgaaatgaa acctggtaaa ggtggacaat
    49501 tgatccgttc tgctggtaca agtgctcaag tacttggtaa agaaggcaaa tacgtattga
    49561 tccgcttgaa ctctggtgaa gttcgtatga tcttagctgc atgccgtgct acaatcggtt
    49621 ctgtaggtaa cgaacaacac gaattaatca acatcggtaa agctggacgc tctcgttgga
    49681 tgcgtaaacg cccaactgta cgtggtagcg taatgaaccc taatgatcac ccacacggtg
    49741 gtggtgaagg taaagctcct atcggacgta aagcaccagt ttctccttgg ggtcaaccag
    49801 ctcttggtta caaaactcgt aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc
    49861 gcaaaaataa ataattactt tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg
    49921 gtcgtagttt aaagaaagga ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc
    49981 aagcaggggt tgaaaagaaa aaagttatca aaacttggtc acgccgttca acagttttcc
    50041 caagttttgt tggatatacc atcgcagttt atgatggacg taaacatgta ccagtttaca
    50101 tccaagaaga catggtagga cacaaattag gtgaatttgc acctacaaga acttatcgtg
    50161 gacatgctgc tgacgataag aaaacgaaac gctaatttga ggggaggata aatccaaatg
    50221 gcagaacaaa tcacatcagc taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa
    50281 gcccgtttag taattgacct tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg
    50341 aaattcacac caaacaaatc tgctggaatc attgaaaaag tattgatgtc agcagttgcg
    50401 aatgcagaaa ataactttga cttagatgtt gaaaacttgg tagtaagcga agcttttgtt
    50461 aacgaaggac ctacaatgaa acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc
    50521 aacaaacgta caagtcatat cacggtagtc gtatcagaaa aataaggagg gataatcagt
    50581 gggtcaaaaa gtacatccaa ttggaatgcg tgtaggcatc atccgcgact gggatgctaa
    50641 atggtatgct gaaaaagagt atgctgagtt cttacacgaa gatttaagaa tccgtaaatt
    50701 tatttcaact aaacttgctg atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg
    50761 tcgcgtaaac atttcaattc acacagctaa accaggtatg gttattggta aaggcggttc
    50821 tgaagtcgaa agcctaagaa aagaattaaa caaattaact ggtaaaagaa tccatatcaa
    50881 tatcgtggaa atcaaaaaac cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg
    50941 tcaattagaa aaccgtgttg ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat
    51001 gcgtgctggc gctcaaggga tcaaaactca agtatctggt cgtctaaacg gagcagatat
    51061 cgctcgttca gaaggatact cagaaggaac tgttcctctt catactttac gtgcggacat
    51121 tgattacgca tgggaagaag cagatacaac atacggaaaa ctaggagtta aagtgtggat
    51181 ctatcgtgga gaaattcttc caactaaaaa aaacactgag aaaggaggga aataatcatg
    51241 ttagtaccta aacgtgtaaa acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct
    51301 aaaggcggaa aagaagtagc attcggtgaa tacggtttgc aagctgttga ttcacattgg
    51361 atcacaaacc gccaaatcga agctgctcgt atcgcaatga ctcgttacat gaaacgtggt
    51421 gggaaagtat ggattaaaat tttccctcac aaatcttata ctgccaaagc aattggggta
    51481 cgtatgggtt ctggtaaagg ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa
    51541 atcatgtttg aaatcgcagg cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct
    51601 tctcacaaat taccaatgaa aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg
    51661 aatgaaggtt aaagaaatca gagaattaac cactgccgaa atgcttgatc aagaaaaaca
    51721 attaaaagaa gaattgttta atcttagatt ccaattagca acaggtcaat tagaaaacac
    51781 tgcacgtatt aaagaagtac gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca
    51841 agctaagtaa ttatggaagg aggccattaa gcgtatgact gaaggaagaa atcaacgcaa
    51901 agtttatcaa ggtcgcgtgg tttcagacaa aatggataaa acaattaccg ttgctgtcga
    51961 aacaaagaaa aaccacccta tctatggcaa acgcatgaac tattctaaga aatacaaagt
    52021 gcatgatgaa aacaacacag caaaagttgg cgacatcgtg agaattatgg aaactcgtcc
    52081 attatcagct acaaaacgtt tccgtttgtt ggaagtagtc gaagaagcag ttattatcta
    52141 ataacacgag attttcctat atagattgaa tgcaaaatct gaaaggagga tacacaacgt
    52201 gatccaagca gaaagtcgtt taaaagtcgc tgataactca ggcgcacgtg aaattttgac
    52261 gatcaaagtc cttggtggat ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc
    52321 tacggttaaa caagcaacgc caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt
    52381 tatcgttcga acaaaatcag gagctcgtcg tacagacggt tcatatatta aatttgatga
    52441 aaatgctgca gtgattattc gtgatgataa aagccctcgt ggaacacgta tctttgggcc
    52501 agttgcacgt gaactacgtg aaaacaactt catgaagatc gtttcactag caccagaagt
    52561 tttataatct tgagacactt atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg
    52621 cgataaagtt aaagttatca ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc
    52681 gttcccgaaa aaagataaag ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa
    52741 accttctcaa gctgctcctc aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc
    52801 taacgtgatg gtggttgact ctactggtgt agctggccga gttggctaca aagaagtaga
    52861 cggcaaaaaa gtccgtgttt ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa
    52921 ggaaggaggg tcttactgaa tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc
    52981 atcattgatg gaaaaattta actatagctc tgttatgcaa acacctaaag ttgaaaaaat
    53041 cgtcatcaac atgggtgttg gtgatgcggt atcaaacgct aaaaacttag ataaagcagt
    53101 tgaagaatta actttgatca ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat
    53161 cgctggattc cgtttacgtg aaggaatgcc aattggtgca aaagttacat tgcgcggaga
    53221 aagaatgtac gaattcttag acaaattggt ttctgtttca ctaccacgtg tacgtgactt
    53281 ccatggtgta agtaaaaaag cctttgacgg tcgtggaaac tatactttag gtattaaaga
    53341 acaattgatc ttcccagaag tagattatga tttagtagat aaagtacgtg gtatggacat
    53401 cgttattgta acaacagcaa actctgatga agaatctcgt gagttgttga cacaattagg
    53461 catgccattc caaaaataat taaaggaggc gaactacgtg gctaaaaaat caatgattgc
    53521 taaaaacaaa cgtcctgcaa aattttcaac acaagcttat actcgttgcg aacgttgcgg
    53581 acgtccacat tcagtttatc gtaagttcca tctttgccgt atttgcttcc gcgaacttgc
    53641 ctataaaggt caaattcccg gcgtgaagaa agctagctgg taaaaagtaa attcgcataa
    53701 gaaggaggta aaagatcaat ggtcatgaca gatccaattg cagattttct aactcgtatc
    53761 cgtaacgcaa acatggttaa acatgagtct ttagaagtac ctgcatcaaa aattaaacgt
    53821 gacatcgctg aaatcttgaa acgtgaaggt ttcgttcgtg atgttgaata catcgaagat
    53881 gacaaacaag gcgtgatccg tgttttccta aaatttggta aaaacggcga acgtgttatc
    53941 acaaacttaa aacgtatctc taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta
    54001 ccaaaagtat tgaacggttt aggtatcgca atcatttcga cttctgaagg tgttgtcact
    54061 gataaagaag ctcgtgaaaa aaatatcggc ggcgaagtta tcgcttacgt atggtaatca
    54121 agtagaatct acaaggaggt gtctttaagt gagccgtatc ggaaataaag tcgttgttat
    54181 ccctgaagga gtaacagtta ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg
    54241 tgaattgact cgtacttttt cagcagatat caaaatgaat atcgaaggaa acgaagtaac
    54301 tttcactcgt ccaaacgaca gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa
    54361 cttcaacaac atggttgtag gtgtcagcga aggtttccaa aaagcgttag aactaatcgg
    54421 ggttgggtac cgtgcacaat tacaaggatc aaaacttgta ttgaacgttg gttactcaca
    54481 tccagtagaa atcacaccac cagctggagt aactgttgaa gtaccagcta acacacaagt
    54541 aattgttaaa ggtgcgaaca aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac
    54601 tcgtcctcca gaaccttata aaggtaaagg tattcgttat gttggcgaat atgtacgccg
    54661 taaagaaggt aaaactggta aataatcgtt tagaaaaatt gcatagaagc cagcagcagg
    54721 aaaaaggcat ttggctgaag tttaccttca aaaaaataca tttttacttg tgccgcagct
    54781 tctatttttc gaaacgtatt tactgcttga aatagtagta caataaaaat aaagaggtga
    54841 caattgtgat tacaaaacca gataagaaca aaacacgtca aaagagacat cgtcgtgtac
    54901 gtaacaatat ctctggtact gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa
    54961 acatctacgc tcaaattatt gatgacgtag cgggtgtaac gctagcaagt gcctctacct
    55021 tggataaaga aatttcaggt ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag
    55081 ttgctgaacg tgcagctgaa aaaggcatta aagtagtagt ctttgaccgt ggtggatacc
    55141 tttaccatgg ccgtgtgcaa gctttagctg aagctgctcg cgaaaatgga ctagaatttt
    55201 agaagaagga ggaataccat tcatggttta tatcgatcca aaacatttgg aattagaaga
    55261 ccgcgttgtt gcaattaacc gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt
    55321 tgctgcttta gttgttgtcg gtgacaaaaa cggacacgta ggatttggta caggtaaagc
    55381 tcaagaagtt cctgaagcaa tccgtaaagc aattgaagat gcgaagaaaa acttagttga
    55441 agtacctatg gttggttcaa caatcccaca cgaagtaatc ggtgctttta gtggtggtag
    55501 aatcctaatg aaacctgctg tagaaggttc tggggttgct gctggtggac cagttcgtgc
    55561 cgtattagaa ttagctgggg tagcagatat cacttctaaa tctttaggct caaatacacc
    55621 aatcaacgtt gttcgcgcaa cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt
    55681 tgcagaactt cgcggcaaat cagttgaaga attaatcgga taaggaggac aacaataatg
    55741 gctgaattaa aagttacttt aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat
    55801 acagttaaag cgttaggtct tacaaagatt aacagttcag tggttaagcc ttctaatgaa
    55861 gcaattaaag gcatgatcaa tactgtttct cacctagtgg acgttgaaga aatttaattt
    55921 agaaaaatca taactagaac attaaggagg tgccaaacct atgaaacttc atgaattaaa
    55981 acctgctgaa ggttcacgcc aagtacgtaa ccgtgttggt cgtggtactt catctggtaa
    56041 cggaaaaact gccggacgcg gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg
    56101 tttaggattt gaagggggac aaacaccatt gttccgtcgt ttaccaaaac gaggattcac
    56161 aaacgtgaac cgtaaagact atgcagtcat taatcttgat gtattgaacc gctttgaaga
    56221 cggagctgaa gtaactcctg ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc
    56281 tggaatcaaa gtattagcta atggggaact aaacaagaaa ttaactgtga aagcagcaaa
    56341 attctctaaa gcagctcaag aagcaattga agcggctggt ggatcaatcg aggtgatcta
    56401 atgtttagac tattaaaaga tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt
    56461 actgtatttg tgttgtttgt atttcgccta ggtgcacata ttactgtacc aggcgtaaat
    56521 gctgcttcat tgcaaaacct ttcttctttg ccatttttca acatgttgaa cttggtaagt
    56581 ggtagtgcga tgcaaaactt ttccattttc tcaatggggg tttcaccata cattactgct
    56641 tctatcgtca ttcagcttct tcaaatggac atcgtgcctc gatttgttga atggtcgaaa
    56701 caaggggaag ttggtcgtaa aaaactgaat caagcgactc gatatctgac attagtattg
    56761 gcatttatcc aatctgtcgg tatcactgct ggattcaatc aatacgctca attaggtttc
    56821 gtgaataatc ctaacgtagc aacttacgta acgatcggtt tgattctaac agccggtacg
    56881 atgtttgtta cttggctagg agaacagatc acagaaaaag gtatcggaaa tggcgtatca
    56941 atgatcattt ttgcaggtat catttctaga ttgcctgaaa gtatcagaga aatttatgaa
    57001 gattacttta taaatattga tccttcagac atatggaaat ctgctatttt tgtggccatt
    57061 ttactagtag cggtcttgtt gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc
    57121 aagatcccga tccaatatac gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg
    57181 ttgaaagtaa atgcagctgg agttattcca gttatctttg ctagttcgtt tattgctacg
    57241 ccaaatgctg ttttacaagc attgagcggt agattcggtt ctgaaaactg gtacaaagtg
    57301 atgatggaga tctttaacta taatacagca cctggtgcta cgatttatac gattctgatc
    57361 gttgccttca ctttcttcta cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac
    57421 ttgcaaaagc aaggaagtta tataccaagc gtacgccctg gtaaggggac tgaagagtat
    57481 gtctcaagat tactgatgcg tttaagtaca gttggtgcag tcttccttgg tttagtggca
    57541 ttgcttccaa tcattgctca aatggtttgg gatcttccac aatcaatcgg attaggcggt
    57601 acaagcttac tgatcgttat cggtgtagct ttagaaactg cgaaacagtt agaaggctta
    57661 atgctgaaac gtaaatatgt tggattcatt gagtaagaaa gcgtgttgag gtttcccctc
    57721 aacacacgtt ctcacacatt tattaggagg gaaaacaatg aacctcattt taatggggct
    57781 gccaggcgca ggtaaaggaa cacaagctga aaaaatcatt gatgcttatg gtattccgca
    57841 catctcaaca ggagatatgt tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga
    57901 agcaaagtct tatatggata aaggtgcatt agttcctgat gaagtaacaa atggaatcgt
    57961 gaaagagcga ctagctgaac cagatacaga gaaaggcttc ttattagatg gctttccacg
    58021 tacgctagat caagcaaaag ctctggacgc aatgctaaaa gatttgaata aaaaaattga
    58081 tgctgtcatc gatatccatg ttggtgaaga aatcttggtc gaacgtctag ccggccgatt
    58141 tatctgcagc aattgtggag caacttacca taaaattttc aaccctacaa aagttgaaga
    58201 cacatgtgat cgttgcggtg ggcatgaatt ctatcaaaga gaagatgata aacctgagac
    58261 ggttaaaaat cgtctggcta tcaacatcaa aaatagtgaa ccaatcttgg cttattataa
    58321 agaacaaggt ttgctaaaca ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga
    58381 tgtacaaaaa atcattgaaa aatagcacga ttgcccgtag ccgacttgca ttatgcagtt
    58441 actcttgtca agttcaataa attatgatag aatatgtcag tccgacttct agagaatcct
    58501 ctagatgatc cattttgaga attttgaggt gggaactgca gtttccgcca ttttatcgtg
    58561 tgaaaatgcg aaacaagtac aaggaggtac tgtgcgtggc aaaagaagat atgattgaag
    58621 tcgaaggtac agtcgtcgaa actttgccga atgcaatgtt taaagttgaa ctagaaaacg
    58681 gacaccaagt tcttgctact gtttcaggta aaattcgtat gcattacatc cgtatcttac
    58741 ctggtgacaa agtaacggtt gaattgtctc catacgatct taaccgtggc cgtattactt
    58801 atcgctttaa ataattgtac tccgtaaaac tacaggaggt attatcatga aagtaagacc
    58861 atcagtaaaa ccaatgtgcg aacattgtaa agtaattcgt cgtaaaggac gcgttatggt
    58921 gatttgccca gcaaatccaa aacataaaca acgtcaagga taattggagg tgctataaat
    58981 atggctcgta ttgcaggagt agatattcct cgtgataaac gtgtagtagt ttcacttact
    59041 tatatctatg gtattggtaa cactactgct aaaaagatct tagctgatgc tggtgtatct
    59101 gaagatgttc gcgttcgtga attaacgaat gaacaaacag atgctatccg tgcagaagta
    59161 gataaattaa aagttgaagg ggatcttcgt cgtgaagtga acttgaacat caaacgtttg
    59221 atggaaatcg gttcttaccg tggaatccgt catcgccgtg gattgcctgt tcgtggacaa
    59281 aacacgaaaa acaatgcacg tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa
    59341 aaataattct taagtgaagg aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa
    59401 cgccgtgtga agaagaatat agaagcaggt attgcacata tccattctac attcaataac
    59461 actatcatca tgatcactga tactcacgga aatgcattag cgtggtcatc agctggttca
    59521 ttaggtttca aaggaagcaa aaaatcaact ccttttgccg ctcaaatggc agcagaaact
    59581 gctacaaaag cagcaatgga acacggatta aagactgttg aagtaacagt taaaggacct
    59641 ggttctggac gtgaagcagc aattcgttca ttacaagcta caggtttaga agtgactgca
    59701 attcgtgacg tgactccagt tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt
    59761 taatgagtgc cgctcatttt gagttttatc agtaacgtca ttgaacaaga cacttttcgt
    59821 tttgaaaggg gtaaagataa gaatgattga gttcgaaaaa ccaagaatcg caaaaattga
    59881 tgaagaaaaa gattatggca agttcatcgt tgaacctctt gaaagagggt atgggactac
    59941 tttagggaat tccctacgtc gtattttatt atcttctttg ccaggtgctg ccatcactaa
    60001 tattcaaatc gatggtgtac tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt
    60061 cactcaaatc atcttgaaca ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa
    60121 aacccttgaa atcgatatta ccggacctgc tacagtaact gctggcgata ttatcgttga
    60181 tagtgatgta gaaattctta acaaagatat gtacatctgt agtgtctctg aaggagctac
    60241 gttccatgct cgcttaacag tgaaacctgg acgtggttat gttcaagcag atgaaaataa
    60301 aaaagaagat atgccaattg gcgttcttcc agtcgattca atctacacac ctgttcgtcg
    60361 tgtgaactat caagttgaga acacacgtgt tggacaccgt gaagattttg ataaattgac
    60421 gatggagatt tggacagatg gttcaattga accgttggaa gcaatgagtt tgtctgcgaa
    60481 aatcatgact gaacatttgg atatttttgt gaacttaact gatgaagcaa aaaatgctga
    60541 aatcatggtc gaaaaagaag aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga
    60601 gttagacttg tctgttcgct cttacaattg tttgaaacgt gcaggaatca atactgtaca
    60661 agaacttaca aataaatctg agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc
    60721 attagaagaa gttaaagcaa aattgcacga cttaggttta ggtttacgta aagaagatta
    60781 aaacctttta cgaaggaggg aaaccaacgt gagttatcgt aaactaggac gcacatctag
    60841 ccaacgtaaa gcgatgttgc gtgatttaac aactgattta ttaatcaacg aacgtattgt
    60901 gactactgaa gctcgtgcga aagaagttcg ctcaactgca gaaaaaatga tcactttagg
    60961 taaacgcgga gatttgcatg cacgtcgtca agctgcggct ttcgtacgta atgaagttgc
    61021 cagcgtgcgt gaagaaaatg aggacatcgt cattgaatca gctttacaaa aactgttcaa
    61081 cgatattgca cctcgttacg cagaacgtca aggcggatac acacgcattt tgaagacaga
    61141 accaagacgc ggtgatggtg caccaatggt tgtcttagaa cttgtctaaa atcacactaa
    61201 taacatcact ttatagatga ttcttttaga gtgttatgat gttggaagat atttttccga
    61261 gtctagctca gcgctgctcc tcaagcgaat atcgcttggg gagtaagcac tcatcataaa
    61321 gtgttttttt ataccttcaa ttacctgtca tgacagatat gtttgaaggc ataaaaaaac
    61381 attttaagcg agaaataatt gaaaattttg tctttttttg atagtgttag taggaacttt
    61441 aggatgaaga aggagtaatc atggagccaa ttattgaatt agaaaagata aattataaat
    61501 accagccaga tgatcttcgc cccgcattga aagatgtctc ctttacaatt gataaaggtg
    61561 agtggatcgc tattattgga cacaatggtt caggaaaatc gacacttgcc aaaacgatca
    61621 atggattgct tctgccagaa tcagggatcg tgaaagtggg aaatcaaata ttggatgaag
    61681 aaaatatttg gacgatccga cagatggttg gaatggtttt tcaaaatcca gacaatcagt
    61741 ttgttggttc gacagtagaa gatgacgttg cttttggtct tgaaaatcaa gggattccca
    61801 gagaagaaat gctagtgcgt gtgaaagatg cacttgagaa agttagaatg gctgagtttg
    61861 cttcacgcga gcctgctcgt ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg
    61921 tggttgcctt acgacctgat atcattattt tagatgaagc aaccagtatg ctagatccag
    61981 aaggtcgtga agaagtgatc tctacgatca agaagatcaa agaagaaagt caattgaccg
    62041 ttatatctat cactcatgat attgatgaag cagctaatgc caatcggata ctagtgatga
    62101 gacaaggcga acttgtccgt gaaggtacac caaaagaaat tttttctgct ggtcctgaac
    62161 tgatcgactt aggtctagat ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac
    62221 gtggggttga tgtgcccagt gaatatatga cagaagaaag gatggtggat tggttatgga
    62281 catccgtttt gaacaagtag acttcactta tcagccgaat acaccatttg aacaaagagc
    62341 cttatttgac atcaatatga cgatcaaaga aaacagttat actgcattag tagggcatac
    62401 cggaagcggg aaatcaacct tacttcagca tttgaatgcg cttgttaagc caacaagcgg
    62461 tacagtccat attggagaac gagatattca gccggatacg gataataaga atttaaagcc
    62521 catccgaaaa aaagttggca tcgtctttca gtttccagaa gcacagctat tcgaagaaac
    62581 ggtagcaaaa gacattgctt tcggtcctaa aaactttggt gtcagcgaag aagaagcact
    62641 agtcctagca aaagaaacat tagaacaagt tgggctggat gaaagctatt tggaacgttc
    62701 gccatttgaa ctttcaggag ggcaaatgcg tagagtagca atcgctggtg tgcttgccat
    62761 gaggccggaa gtacttgtat tggatgaacc aacggcagga cttgatcccc agggcagaaa
    62821 agaaatgatg gagatgtttt ggcgtcttca taaggaacag cagatcacta ttgtattagt
    62881 tacgcattta atggatgatg tagccaattt tgcagattat gtctatgtgt tggaaaaggg
    62941 acgaatcgtt aatagcgggg agccgcaaga ggtctttcaa aacattgaat ggctaaaaga
    63001 aaaacaacta ggcgtaccta ctgccaccga gtttgcagaa gaattaatgg ctaaagggat
    63061 gaactttgct accttgcctt tgacagctga agaattagca gacgctatcg tccattatgc
    63121 tggaggaaat gcccatgatg aataaattga tatttggtcg ttatatgcct ggagattcct
    63181 tgatccatcg attggatccc cgagctaaat tattagccag tttttatttt atcggaatca
    63241 tttttttagc aaataattgg gaaacgtacc ttttgttggc atttttcaca ttactttctg
    63301 tcttcttgtc aaaagtagat ttgaaatttt ttatccgagg cattcgccca ttgatctggc
    63361 taatcctctt cactgtagct ttgcaggtct tgtttacaag tggcggagaa acttattgga
    63421 gttggggtgt tttccatatc actgaatttg gtgtgatgaa cggcctcttt attttctgtc
    63481 gattcgtgtt gatcattttt atgtcgactc tactcacgct tactacaccg cctttggaat
    63541 tatcggatgc gattgagtac ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg
    63601 aaatttcatt gatgctgtcg attgctcttc gttttgtgcc tacattgatg gacgaaacag
    63661 aaaagatcat gaacgctcag cgcgctcgcg gtgttgattt tggtgaagga aacctagttc
    63721 agaaaatgaa agcagtcatt cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg
    63781 cggaagatct tgcgactgct atggaagcaa gaggctacaa aggtggggaa ggaagaacga
    63841 aatatcgtgt attgcattgg cataatagag acacgatggt tatcattgct tttgtattat
    63901 taactgtgct actgatcttt ttaagaggat aaacgttcag tgggccgcta tctattcttt
    63961 tcttgctgat ctgtttgcaa gaaaggatgc aagcagtgta tgacaagaaa tattagggtc
    64021 tgtggcagac taaggtcaca ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt
    64081 ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta
    64141 cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca
    64201 aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa
    64261 gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta
    64321 atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc
    64381 cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca
    64441 agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt
    64501 ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag
    64561 cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc
    64621 tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa
    64681 gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta
    64741 gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt
    64801 attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg
    64861 tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa
    64921 ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc
    64981 tacttctgga acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta
    65041 gagaaacaaa cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg
    65101 tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
    65161 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
    65221 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
    65281 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
    65341 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
    65401 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
    65461 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
    65521 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
    65581 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
    65641 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
    65701 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
    65761 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
    65821 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
    65881 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
    65941 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
    66001 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
    66061 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
    66121 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
    66181 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
    66241 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
    66301 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
    66361 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
    66421 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
    66481 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
    66541 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
    66601 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
    66661 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
    66721 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
    66781 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
    66841 ccaaaatgat tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc
    66901 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
    66961 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
    67021 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
    67081 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
    67141 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
    67201 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
    67261 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
    67321 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
    67381 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
    67441 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
    67501 gaagataaaa aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
    67561 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
    67621 caagaaacca aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg
    67681 catctggcat catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat
    67741 agtaagaaat taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac
    67801 tttgctttcc aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg
    67861 catcacttgc caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa
    67921 gtgctaaaat caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa
    67981 agaatgactt taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt
    68041 cagtttatca tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata
    68101 aaatttagaa taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg
    68161 gtaggaatca aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct
    68221 gaacggtcca agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag
    68281 ttgacaccca aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag
    68341 gtttaacagt acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct
    68401 tgcgacctgt aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga
    68461 gcaatagaat aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag
    68521 tacattttca tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat
    68581 acctgaaatt ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg
    68641 ctatgataaa atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc
    68701 atggctgtaa aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca
    68761 acagttgttg gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat
    68821 caaattaaag ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt
    68881 gtccgccaag aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg
    68941 aaaatctctg aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta
    69001 ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac
    69061 taggcaggag actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt
    69121 gtaacagaaa taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg
    69181 caagtaaatg cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg
    69241 ggaacaaata tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca
    69301 gaaaaagtag gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga
    69361 aattccggcg ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt
    69421 gtctcattaa atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat
    69481 aaagcagtaa tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa
    69541 gctggagaaa agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta
    69601 aaaggagcaa agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc
    69661 ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca
    69721 ttgaatggag aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat
    69781 atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat
    69841 tttggttact gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca
    69901 ttcgattacg aagaattccg taactattta gatggtatag gagattcttt acttgtggtc
    69961 aatgatgacg agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat
    70021 tatggacaaa aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat
    70081 gagacgattt tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt
    70141 atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt
    70201 ttaggcgcta attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt
    70261 gttaaagcga ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac
    70321 atcttcatgg cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct
    70381 tcaagaacga tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta
    70441 gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact
    70501 cacgcgatca gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc
    70561 atgatcgatg gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca
    70621 ctagaacaaa tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga
    70681 acagttgaac aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa
    70741 atcgagatcc accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt
    70801 ctgtgtcaga atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc
    70861 aggtatgatc gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt
    70921 ctttagatga tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg
    70981 ccagcttggg catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag
    71041 atattcagga aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa
    71101 tcgtagtctc gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc
    71161 gtatgatgca agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag
    71221 aaaaagtcat tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag
    71281 ccttgaacgg gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc
    71341 acgtcaacaa atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag
    71401 aatacggcac attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc
    71461 ttgaccgagc ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc
    71521 aagccaaacg acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat
    71581 tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat
    71641 tgaaacaatt tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga
    71701 atgaaatctg tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg
    71761 taggtagcgg aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt
    71821 tccaaggtgc gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc
    71881 aacagctgtt tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa
    71941 aagaacgacg tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca
    72001 cacatgcatt gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg
    72061 agcagcaccg ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag
    72121 atgtcttatt tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg
    72181 agatggatgt atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct
    72241 gggtaagaac acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag
    72301 gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa
    72361 atgcagtgga aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct
    72421 tgttacatgg taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca
    72481 accagatgca gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg
    72541 cgaccgtcat gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc
    72601 gtggacgtgt tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa
    72661 atgaactagg tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa
    72721 gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc
    72781 tgcctcaatt tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag
    72841 aagaggctgc agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt
    72901 tagcgcaaag cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc
    72961 tatactttat actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa
    73021 tgggcggaga tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt
    73081 ttccagaagt agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa
    73141 atgaaaaaaa catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag
    73201 taaaagcaat tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa
    73261 acggtgaagc agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt
    73321 tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag
    73381 tagtagggaa agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca
    73441 aaccagaaca tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag
    73501 gtatcggacg tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg
    73561 aagtgacgaa aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa
    73621 acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta
    73681 caggcaacgc tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa
    73741 aatctgctat tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg
    73801 gcttacgttc tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg
    73861 gcttgaaagc accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata
    73921 cgatccgcca aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat
    73981 ttgaaaaaag cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag
    74041 gtggacattc ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta
    74101 tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt
    74161 atttcaaatc attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat
    74221 gatttaaaag ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc
    74281 ggtacggaaa tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat
    74341 tatatcagca gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat
    74401 gcagcttttt atccttcttt ttttaatctt tttgacacg
//
LOCUS       DAGCXG010000005        66424 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGCXG010000005 DAGCXG010000000
VERSION     DAGCXG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.005"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            103..273
                     /locus_tag="MB022_000325"
     CDS             103..273
                     /locus_tag="MB022_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465354.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            493..1200
                     /locus_tag="MB022_000326"
     CDS             493..1200
                     /locus_tag="MB022_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4465355.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="MB022_000327"
     CDS             complement(1259..1903)
                     /locus_tag="MB022_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBT4465356.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="MB022_000328"
     CDS             2030..2317
                     /locus_tag="MB022_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465357.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="MB022_000329"
     CDS             2425..2745
                     /locus_tag="MB022_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4465358.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="MB022_000330"
     CDS             2826..4139
                     /locus_tag="MB022_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4465359.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="MB022_000331"
     CDS             4136..4855
                     /locus_tag="MB022_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBT4465360.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="MB022_000332"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="MB022_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBT4465361.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="MB022_000333"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="MB022_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBT4465362.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="MB022_000334"
     CDS             join(8416..8487,8489..9517)
                     /gene="prfB"
                     /locus_tag="MB022_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBT4465363.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="MB022_000335"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="MB022_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBT4465364.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="MB022_000336"
     CDS             10292..11185
                     /locus_tag="MB022_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465365.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="MB022_000337"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="MB022_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4465366.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="MB022_000338"
     CDS             13122..13979
                     /locus_tag="MB022_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBT4465367.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="MB022_000339"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="MB022_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBT4465368.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="MB022_000340"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="MB022_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBT4465369.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="MB022_000341"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="MB022_000341"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4465370.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="MB022_000342"
     CDS             16735..17493
                     /locus_tag="MB022_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4465371.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="MB022_000343"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="MB022_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBT4465372.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="MB022_000344"
     CDS             18406..19437
                     /locus_tag="MB022_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4465373.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="MB022_000345"
     CDS             19434..20201
                     /locus_tag="MB022_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4465374.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="MB022_000346"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="MB022_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBT4465375.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22166..22486
                     /locus_tag="MB022_000347"
     CDS             22166..22486
                     /locus_tag="MB022_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBT4465376.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22486..22836
                     /locus_tag="MB022_000348"
     CDS             22486..22836
                     /locus_tag="MB022_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBT4465377.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23003..23941
                     /locus_tag="MB022_000349"
     CDS             23003..23941
                     /locus_tag="MB022_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBT4465378.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGNN"
     gene            23956..24786
                     /locus_tag="MB022_000350"
     CDS             23956..24786
                     /locus_tag="MB022_000350"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBT4465379.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24830..25855
                     /locus_tag="MB022_000351"
     CDS             24830..25855
                     /locus_tag="MB022_000351"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4465380.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25870..26808
                     /gene="galU"
                     /locus_tag="MB022_000352"
     CDS             25870..26808
                     /gene="galU"
                     /locus_tag="MB022_000352"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4465381.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26933..27373
                     /locus_tag="MB022_000353"
     CDS             26933..27373
                     /locus_tag="MB022_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBT4465382.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     MTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27376..27978
                     /locus_tag="MB022_000354"
     CDS             27376..27978
                     /locus_tag="MB022_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4465383.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     ISEAKPVADDTKEMMKMAKEDAKEAKKEIKDTITDSSTNH"
     gene            complement(28037..29143)
                     /locus_tag="MB022_000355"
     CDS             complement(28037..29143)
                     /locus_tag="MB022_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4465384.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29353..30354
                     /gene="ccpA"
                     /locus_tag="MB022_000356"
     CDS             29353..30354
                     /gene="ccpA"
                     /locus_tag="MB022_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBT4465385.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30690..33035)
                     /locus_tag="MB022_000357"
     CDS             complement(30690..33035)
                     /locus_tag="MB022_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4465386.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33314..34216
                     /locus_tag="MB022_000358"
     CDS             33314..34216
                     /locus_tag="MB022_000358"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBT4465387.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            34429..36204
                     /locus_tag="MB022_000359"
     CDS             34429..36204
                     /locus_tag="MB022_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBT4465388.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRIYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPLFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLTIVFDFLQSGYAYAWRYKDFHQGENYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36270..37856)
                     /locus_tag="MB022_000360"
     CDS             complement(36270..37856)
                     /locus_tag="MB022_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBT4465389.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            38107..38331
                     /locus_tag="MB022_000361"
     CDS             38107..38331
                     /locus_tag="MB022_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBT4465390.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38473..40224
                     /locus_tag="MB022_000362"
                     /pseudo
     CDS             38473..40224
                     /locus_tag="MB022_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            40224..42008
                     /locus_tag="MB022_000363"
     CDS             40224..42008
                     /locus_tag="MB022_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465391.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQRITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            42334..43185
                     /locus_tag="MB022_000364"
     CDS             42334..43185
                     /locus_tag="MB022_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4465392.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYNIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLTEAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            43280..45166
                     /locus_tag="MB022_000365"
     CDS             43280..45166
                     /locus_tag="MB022_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4465393.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILTAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPNLSLEVLSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKDEADETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTNYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(45206..46108)
                     /locus_tag="MB022_000366"
     CDS             complement(45206..46108)
                     /locus_tag="MB022_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4465394.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDIDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFGNKEVE"
     gene            46339..47742
                     /locus_tag="MB022_000367"
     CDS             46339..47742
                     /locus_tag="MB022_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4465395.1"
                     /translation="MAKGIKITTIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KGKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNEGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSSNTHIMEEV"
     gene            47742..49175
                     /locus_tag="MB022_000368"
     CDS             47742..49175
                     /locus_tag="MB022_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4465396.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            49320..50246
                     /gene="trxB"
                     /locus_tag="MB022_000369"
     CDS             49320..50246
                     /gene="trxB"
                     /locus_tag="MB022_000369"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBT4465397.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            50459..52186
                     /locus_tag="MB022_000370"
     CDS             50459..52186
                     /locus_tag="MB022_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBT4465398.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQSLLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            52201..52551
                     /locus_tag="MB022_000371"
     CDS             52201..52551
                     /locus_tag="MB022_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBT4465399.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(52643..53503)
                     /locus_tag="MB022_000372"
     CDS             complement(52643..53503)
                     /locus_tag="MB022_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBT4465400.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTENSD"
     gene            53595..54023
                     /locus_tag="MB022_000373"
     CDS             53595..54023
                     /locus_tag="MB022_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4465401.1"
                     /translation="MKKQTLIEQTLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            54185..54361
                     /locus_tag="MB022_000374"
     CDS             54185..54361
                     /locus_tag="MB022_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBT4465402.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            54388..54834
                     /locus_tag="MB022_000375"
     CDS             54388..54834
                     /locus_tag="MB022_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBT4465403.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(54988..56367)
                     /gene="brnQ"
                     /locus_tag="MB022_000376"
     CDS             complement(54988..56367)
                     /gene="brnQ"
                     /locus_tag="MB022_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4465404.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEACELPEE"
     gene            57072..58043
                     /locus_tag="MB022_000377"
     CDS             57072..58043
                     /locus_tag="MB022_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBT4465405.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            58067..60259
                     /locus_tag="MB022_000378"
     CDS             58067..60259
                     /locus_tag="MB022_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBT4465406.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELTVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            60276..60752
                     /gene="ybeY"
                     /locus_tag="MB022_000379"
     CDS             60276..60752
                     /gene="ybeY"
                     /locus_tag="MB022_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBT4465407.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            60736..61131
                     /locus_tag="MB022_000380"
     CDS             60736..61131
                     /locus_tag="MB022_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBT4465408.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            61146..62045
                     /gene="era"
                     /locus_tag="MB022_000381"
     CDS             61146..62045
                     /gene="era"
                     /locus_tag="MB022_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBT4465409.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            62188..63000
                     /gene="recO"
                     /locus_tag="MB022_000382"
     CDS             62188..63000
                     /gene="recO"
                     /locus_tag="MB022_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBT4465410.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            63384..64301
                     /gene="glyQ"
                     /locus_tag="MB022_000383"
     CDS             63384..64301
                     /gene="glyQ"
                     /locus_tag="MB022_000383"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBT4465411.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANSENMKEAE"
     gene            64303..66378
                     /locus_tag="MB022_000384"
     CDS             64303..66378
                     /locus_tag="MB022_000384"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBT4465412.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRVIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        21706 a        11731 c        14175 g        18812 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga aaggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagatacg gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgtttacca agagattatg aacttgccac ttatcgccga
    22021 gtcacaagga ataatttcaa aaaaagctcg tcacgtttta tgaatatggt aatttgtgac
    22081 cgagaatttt gcagagagac accgattaag aagaaggttg tcacaacgat tttcttcatt
    22141 taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa
    22201 tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt
    22261 tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa tcattttata
    22321 cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa
    22381 caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg
    22441 caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa
    22501 cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc
    22561 catgtaagca gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg
    22621 tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg
    22681 tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa
    22741 cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc
    22801 aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg
    22861 cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa
    22921 acgttttttt taattggtag tacttttggc tagacaatga taaaatgata gagaacactc
    22981 taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa
    23041 attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg
    23101 ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg
    23161 tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg
    23221 tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg
    23281 attaccagct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg
    23341 ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct
    23401 tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat
    23461 tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggtca
    23521 tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg
    23581 agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt
    23641 aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta
    23701 tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt
    23761 tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt
    23821 tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc
    23881 aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacaacta
    23941 gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt
    24001 cctatctact ggtatgcatt gatcatcgta tcaggtatca ttattgccat gtggcttagc
    24061 agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg
    24121 ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag
    24181 tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc
    24241 ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg
    24301 tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg
    24361 ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt
    24421 ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca
    24481 acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga
    24541 aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt
    24601 ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc
    24661 cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg
    24721 cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt
    24781 atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag
    24841 agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa
    24901 cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca
    24961 acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac
    25021 caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc
    25081 aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca
    25141 tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga
    25201 aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga
    25261 agtagcgaaa catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa
    25321 atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat
    25381 cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca
    25441 cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat
    25501 cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggagt
    25561 aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt
    25621 gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg
    25681 tgtgaatact actcaagcag cgatggaact agcccaaata atggaaatcg aaatgcccat
    25741 cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga
    25801 aatcatgtta cgtgatggga aaaaagaaaa tgaattctca ttaacaattt tttgaaggag
    25861 cgaatgtata tgaaagtcaa aaaagcagtt attccagcag caggattagg aacaagattt
    25921 ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc
    25981 cagttcatcg ttgaagaagc gttgaaatca ggaatcgaag acatcttgat cgttacagga
    26041 aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta
    26101 agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg
    26161 catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc
    26221 tttattggaa atgaaccgtt tgtcgttatg ctaggcgatg atatcatgga agatgaagta
    26281 ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac gatcgctgtt
    26341 atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg
    26401 gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca
    26461 agtgatttag ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc
    26521 gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat
    26581 aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa
    26641 tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct
    26701 ttacgtgagt atatcctgtc tttaggtaaa aaattagaag cagaaggaag caagatcgat
    26761 cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta
    26821 acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa
    26881 aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg
    26941 aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt
    27001 tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc
    27061 aaatatgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg
    27121 cttgctggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga
    27181 tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag
    27241 taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc
    27301 cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac
    27361 aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggagga
    27421 acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat
    27481 gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg
    27541 aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa
    27601 caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca
    27661 ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat
    27721 cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct
    27781 aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat
    27841 actgtcaatg aaggaatatc agaagcaaaa ccagttgcag atgacacaaa agaaatgatg
    27901 aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aataacagat
    27961 tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    28021 ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta gtcgtgaatg
    28081 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    28141 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    28201 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    28261 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcaatttga tcaagttcac
    28321 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    28381 tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    28441 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    28501 tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    28561 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg
    28621 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    28681 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    28741 ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    28801 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    28861 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    28921 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    28981 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    29041 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    29101 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    29161 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    29221 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    29281 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    29341 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    29401 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    29461 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    29521 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
    29581 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    29641 atcttagcaa attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg
    29701 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    29761 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    29821 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca
    29881 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
    29941 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    30001 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    30061 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    30121 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    30181 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    30241 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    30301 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    30361 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
    30421 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    30481 caccgtttat tcgggaaaag gggttatgac aaaattttgt tataatcctt ttttttgtct
    30541 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    30601 atgctgcatt atgttcacca ccatcaaaaa aataagggac tgtgacaata ctattgtcac
    30661 aatcccttat tactgtgtat tctattaaat caatcatctt tctcatctga ttctttggtt
    30721 gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg
    30781 gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat
    30841 tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt
    30901 tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc gaatttctca
    30961 tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa
    31021 ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag
    31081 ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta ttcattggta
    31141 gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca
    31201 tctttgaatg gtgtagtgat ttttgcattg atcacactgc gcatcatgtc attcatgatc
    31261 gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt
    31321 cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct
    31381 aaagcctgaa atccattggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca
    31441 gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa
    31501 gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata
    31561 ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta
    31621 gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat
    31681 acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt
    31741 ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg
    31801 taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt
    31861 ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt ttctacgcca
    31921 ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt
    31981 tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca
    32041 gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta
    32101 taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta
    32161 aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata
    32221 ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcacct
    32281 gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct
    32341 tgcggcagcg tcacatcttt tgcatctaca ccaaagattc ctcttgcagc ttcctggact
    32401 ccagcaatgt tttgtccctt attattacga ccaaatggcg agacattcaa atatgtagca
    32461 atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcatttgct
    32521 ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa
    32581 gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct
    32641 ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt
    32701 ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt
    32761 tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt
    32821 tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag
    32881 ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca
    32941 gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    33001 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    33061 atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga
    33121 atttcttttt taaaggagtt aaaacgtgag aaaatgatct tttttataaa agagttctta
    33181 agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca
    33241 ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag
    33301 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    33361 aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc
    33421 ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta
    33481 catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg
    33541 gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt
    33601 tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc
    33661 aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga
    33721 acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac
    33781 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    33841 ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt
    33901 aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga
    33961 ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat
    34021 cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat
    34081 cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat atggaaaaga
    34141 aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt tattagcaga
    34201 agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc
    34261 ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtctttttc atcatgcatc
    34321 ttctcccccc tctttttaaa tttagacaaa cagggtatac tggtagaaaa gtaaaaaaac
    34381 gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact
    34441 ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc
    34501 ctattattta caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg
    34561 tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa
    34621 tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta
    34681 gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt
    34741 gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta tcttctttta
    34801 agtatctatt tttatttttt gttttctccg attagtttta cacatgaata ttccactcga
    34861 atatatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg tttactagct
    34921 atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt agggctttct
    34981 gtcgcttttc ctttattttg gttcattcgc gaagattcta tctggcttct tccttttact
    35041 gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt ttctttcaaa
    35101 gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg gctgaaactt
    35161 tcattgtttt tacttcctat agttggcttt tccatcgtct atcagtggat cgaatataaa
    35221 aatgagacaa catacggtat tgcaaccatc aatgaccgtt cgagtggaga atttggacgt
    35281 ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga aaacagtgag
    35341 atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac attccatact
    35401 gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg tggaaaagta
    35461 aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag cgaatctggt
    35521 tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa ccaagaatta
    35581 gaagaagcct atcacaaggg aaccatcaaa aagaaaaaag agttctatct gatgggatca
    35641 ggagatggaa agacgatgag tgatctgaca atcgtattcg atttccttca atcaggatac
    35701 gcttatgcat ggcgatataa agatttccat caaggagaaa attatagttt cggaacgcaa
    35761 gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa ttggatcgat
    35821 agtaatgaac cgagaacacc taatttccaa ttgacaaaat cagcaaaagt cgcgaatgtc
    35881 atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat agcagggata
    35941 ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta tagttcctct
    36001 ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg tgtctcgtgg
    36061 ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac tggcgcaggc
    36121 gtgccgttaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attaaatctg
    36181 aaaaaagctt tatcttctaa ataaaaagac ccttggtagc tagattttta acctaactat
    36241 caagggtctt tgtgtggaaa aatcagtttc tattcgattt cttctaccgt ttcctgcaaa
    36301 cgctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc caacaggaga
    36361 aggacaagaa caatgattac ataagcgatt tgatcagcca atgctagttg agctgaagtg
    36421 gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtc
    36481 agactaatca cagagaacca gccaagaagt tttacaacaa atgaattttt gaatcggctc
    36541 cccatctccg cttggctgtt ggtcatcatc agtaaaggga tcatcgaaaa cggcaacgca
    36601 aaagccaaga acacctggga ttcattcatt aagttattca aagcggtatg ttcttcgatc
    36661 gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga aagcaatcgt
    36721 gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc catgatgact
    36781 tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag tgctacagca
    36841 aataaagtgg acaagatacc agattttgct acagcaatca agataccatt gctcatagct
    36901 gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac agaaccggtt
    36961 ttgaagacag cgacacccat gatcaatagc agtgcattga ctagaaaagc catcgacaat
    37021 tggatattag agtcccaagt agaaaaacgt acagcttgtg ctacatcttc ttcgtcttgg
    37081 tgatcgatct tccgagtttg tgaaactgct gaatgtagat ataagttatg tggcatgact
    37141 gttgcgccaa taattcctaa tgctccagtt agcggagttt cgccaccaat gacaggtgag
    37201 tcggagaatg tatggctgtt agggataaag ccagcaaata aactgtgcca atctgggtta
    37261 gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaatcaagca gacaaccagt
    37321 gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac atctaataca
    37381 gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc gattgctgca
    37441 ccaattactt ctgcaatatc tgttgccata atagctaatt cagtcaatat ccataaaatg
    37501 acacctaatg tcttacttgt gcgtgctcgg attgcttggg ccaaatccat ttggctcacg
    37561 attcctaatt ttgctgccat atactgtaaa agcatagcaa taagactaga catcaaaata
    37621 actgacatca ataaatattg gaaattttgt cctccggtga tcgaagtaga ccagttaccc
    37681 gggtccatat acccaactgc tactaacgca ccaggtccag agtaagcaag aagtgtttta
    37741 aaaaaacttt tcccctttgg tacatcaact gttccattta cttcagctaa agataaacca
    37801 tttgtatgtt caataagtcg atgcttatga aattgtttgt tggattgatt attcaagaaa
    37861 aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa aaaattaaag
    37921 aaaaagattc ggtatgccta atttattttc gtggcgcgtc ttgctgaatg acgaaagaaa
    37981 ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat acgcctttct
    38041 tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata gaaaggagag
    38101 atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt gctaatcggc
    38161 gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga aaaaaatcct
    38221 cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa accatctgag
    38281 aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc
    38341 caacgacttt ttacgggctg attttttcag cccgtttttt atttcgtaat ttgttagact
    38401 attcagatta ttcagcaaaa aagacaagca cagacaatcc taggctcttg ataaagacaa
    38461 aggaggagta aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca ggaaaagaaa
    38521 cattatttaa ttggagtttt ctcgctattc gcagtggcct tggtacagtt agttcctcca
    38581 aaagtgattg ggatcatcat tgatgagatt gccgataaaa atttatcaat aaagatcatt
    38641 ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt tcgttatatc
    38701 tggcggacga atatttgggg aaatgcagct cggttggaga aagaacttcg gcagcagtta
    38761 ttcaaacatt ttacataaat ggacaatctc ttttaccaaa aatatcggac tggggatttg
    38821 atggctcatg caacgaatga tttgaatgcg atccagaatg tagcaggagc tggtatcttg
    38881 acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat tttgtttatt
    38941 gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt cacttcacga
    39001 gtgctggggt cgaaactcca tgatgctttt cgtgattcgc aagaagcttt ttctagtatc
    39061 aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt tggacaagaa
    39121 aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa aaacaaacga
    39181 gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg tctgtcttat
    39241 gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc gatcgggcag
    39301 atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc gattggtcga
    39361 ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga acttttgcat
    39421 gaaaaaacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa aggcgagctg
    39481 aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt agaacaaatc
    39541 aagtttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa
    39601 acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa aatccaattt
    39661 ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat cggctatgtg
    39721 cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt ttctaatcca
    39781 aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa caatgaaatc
    39841 aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc tttatctggc
    39901 ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga attattaata
    39961 ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt gcataattta
    40021 aaagaaataa gaaaagagaa aacaactatc atttcagctt accgtttaag tagcgtgatg
    40081 catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat
    40141 gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca attagaagcg
    40201 aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg acaaaatcga
    40261 ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc aaacctttta
    40321 gaagaacgtt catcgttgct atcttatttg cgtttgctct gtctgtgatc aatgtcctat
    40381 tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg gctacgacac
    40441 aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa agtatcattt
    40501 ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag tatattaggg
    40561 taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag acaccggcag
    40621 gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt tggtacgtct
    40681 ttttgatggt attgactggg atctttgcag tcgtttcttc attcattgcc atgtttcaaa
    40741 tcaatggaga aattgccttg tggactttgg tgttcttgcc aattttagga atcgtcatct
    40801 ggtattacca gaaatttagt tctcgtattt accggaatat gcgagaaaaa ttaagtcagc
    40861 tgaatacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag tttcgccaag
    40921 aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac acgcgctatg
    40981 cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta tatgcattgg
    41041 cgattgcgtt ggcgttgact ttatttggga tcgatgcgtt gcattctccc gtagaagttg
    41101 gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg tctcaaatga
    41161 tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc ttgaagatta
    41221 tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg attacttcag
    41281 gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat gtattaaaca
    41341 atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac accggaagtg
    41401 ggaaaagctc gatcatcaat gtgttgatgc ggttttacga attttatgaa ggtcagatat
    41461 tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag aaaatggggc
    41521 tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt cggatgatga
    41581 atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa gcaaacaaat
    41641 tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt gccagctatt
    41701 ctagtggaca acgacagcgg atcacttttg cgcgaacaat cgtgactgat cctaaaattc
    41761 tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg atccaagaag
    41821 gattggcgaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg ctgtcaacga
    41881 ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa cgcgggaatc
    41941 atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg caaagcagtg
    42001 gtatataaaa gatgttgtga cagtagcatt ttgacctaag cactagagtg aaaataaaac
    42061 atagcttctc atatttttca tctgtgagag ttagtgtcgt agtcaccaga tttaaaaaaa
    42121 gattcgacag ctagtcagaa gtgcctaata gccactaaga aagcgttgac aaaagcattt
    42181 actttcgata atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa
    42241 acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt ttgttttgga
    42301 tacagcccta tacatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac
    42361 aacgtcgtaa taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg
    42421 gcattcaaaa agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag
    42481 ttgacaaatg acggactttc acaacagttc caagaattgg cacaagcgat tcctcttgag
    42541 tacttagaaa tttcttgtga aattattgaa tatacaaaag aggctctcca agtccagttg
    42601 aatgattcgg tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa
    42661 atgggaatat ttgtacccaa tgtattgctg tataatatca aacaactatt tcctaaagag
    42721 tatgccatcg ggaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca
    42781 gaagatgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccag
    42841 caagacaata cctacctgtt taccacaaca atacaggata ttttaaatat cgtccgctat
    42901 tactataaga ttacatttga tgaagattca gtccactttt ttcgattcgt cacacacctg
    42961 cgtttttttc tgacacgcgt cctagatgga gcgaaatcag aagtgggaga agtttctgag
    43021 gaattgttcc aagttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata
    43081 gcagactttt taaccgaggc tttcgactac cagatgtcca cggatgaaaa aatgtattta
    43141 acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaggaggaca
    43201 aattaacacg aacgtatgaa ataagggtag tgacattcag ttggctaaac cttccactaa
    43261 ttaattggag ggaatagaaa tgggaaaata ccaagaatta gcgagcaaaa ttgtagaaaa
    43321 tgtcggcggg aaggaaaata tcaatggttt gacacattgt atcacaaggc ttcgtttcaa
    43381 attaaaaaac gaagaaaaag caaatgatga gatcttgaaa aatatggacg gtattgtgac
    43441 ggtaatgcga gcgggaggac agtaccaagt tgtcatcggg aatcatgtgc cagttgtgtt
    43501 tgaagaggtt ataaaagcag gaaaccttac ttttgatgag gctgtttcaa cagaaaaaat
    43561 gagaccgttt gatatgctta ttgatggaat atcgggatgt ttccagccat tccttgctat
    43621 cctgactgct ggtgggatga ttcgtggact gacagcatta cttgtcttct taggcgcgtt
    43681 tgatcgcggt tctggaacat ttgttatgtt cgacaatatc ggtgattctg tattccaatt
    43741 tatgcctgtt attatcggtt taacagctgc ccgtaaattt aaagtaaatg aattcgtagg
    43801 gatgttgctt ggcgcagcat taatgaatcc taacttgtcg ctggaagttt tgagtggtgc
    43861 agcagaagca ccactgacga ccatattttc tggtactatt tttgaagcac ctatttatca
    43921 aacagtattt ggcattccct ggattgcgcg taactacgct tcttctgtca ttcccattat
    43981 ctttattgtg ttgttagcta gccaaattca gaagccaatt aaaaagttag tacctgagat
    44041 gatcgcgaat ttcttcgttc cattcttcac cgttcttatt acaatgccct taggtttttt
    44101 actcgttgga ccagcattca cattcgcaac tgatatttta atggctggat ttgaaacact
    44161 tttagcactt tcgccagtta tttatggagc aatcgtaggc ttcttctggc aaattctggt
    44221 gatgtttggt ctccattggg caatcgtacc aatgggattg atgcaatttt ctgtgaatgg
    44281 ctggcaaaat atcatgacac ctgttgcagt agtgtctttt ggacaaactg cagcacttac
    44341 tgcactctac tttaagctta gaaacccaaa agacaaagca atcgcaatac cagccattgt
    44401 ttctggtatt gtggggatta cagagccagc tatttatggg tttacattgc cacgtaaaaa
    44461 aatatttatt tttacctgtg tcggtggtgc gttagggggc gcgtatagtg ggttaatgaa
    44521 tttaacttct tggaatcaag gtggactagg catctttaca attcccaact atattagacc
    44581 agatggtgat ttaacagatg tgattaatgt attgattggg atcgccattg cgatggtggt
    44641 atcctttgcg ctgacttttt tcttttggaa ggatgaagca gatgaaacag atgctataca
    44701 gaaaaaatca ggtaaagaaa ttgtaaaaac accgatacaa ggacaaatcg ctccactaaa
    44761 tgcagcgaag gatgcagctt ttgctcaagg aacattaggt cgagggatcc tgatctaccc
    44821 tgaaaaagga gaagtacgtg ccccttttga cggaacagtt atgaccctgt tcccgacgaa
    44881 acatgcaatc ggaatggttt ctgaaacagg gctggaattg ctgattcatg tcggattgga
    44941 tactgtgcag ttggaaggaa aatattttga aagcttagtg cagcaaggag aaagagtgaa
    45001 acaaggcgac ctgttgctac gcttcgacaa aaagaaaatc gaaaaggaag gatacagttt
    45061 agaaacacct gtgatcgtca ccaattacac gaattatttg gatattttgg aaaaccaagg
    45121 ggagacggtc gggccagacg atgcacttat caccgcactg acctgaacgg ttaggtgaag
    45181 aatggactag gcttaactat tctttttatt ccacttcttt atttccaaaa ttgaaaggct
    45241 ctttcatgat ggctgagctg atagggcgtt tatcgaattt ttgaccgaca gaaattagat
    45301 ggactaaatt ctgttgataa ttttctgcaa aaacacaact atataacacg tctagaagat
    45361 aacaaattga tgtattgatc gtaaaattac caatttttga atacagtcgc tcgcgtgtcg
    45421 taagtcgcaa agcggctgta ctcattgcag atagcgttga atcaccgata ctggtcaaag
    45481 ctaaaattgg aacatgattt tgtttcaata tttctgcggt acgaagaatc actttatttt
    45541 cacccgtata agaaatcagg atcccgcaca tatcactcgt gcaattgtac gcttcataga
    45601 acgattcacc aaaagtggtg acaatcgtga catccatttt gatgcgcttc attttcagca
    45661 tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa aatcacgatt ttttttgctt
    45721 gaagcattag ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa agtgtatctt
    45781 gaatcgtcat ctgttctaaa gaagccattt tatttgcgat cgtcataata ccgtctgtgg
    45841 tggaaaaagg aagattcgca tctatgtctt gaaaattccc ttcaagatag gctgtttcag
    45901 ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc catttttttt gctacacgga
    45961 tgagtgtgga ggggtggaca taacatcgct cagcaatcgc ttggatggtc atttcgccaa
    46021 gtgctggaga atcgcttaag agaaactgga tcacttcatt ttctgaagga gaaaaggaga
    46081 tttctttgat tttttcacgt aataacatgt agataccttc ttcttatttt tttacgtaaa
    46141 ttataacttt tttgaaaatg atttgtaaac ggatttatac tatataactt aaaatagaca
    46201 ttttttgaca agatatttac aaaaatccac tagaaaacag taaattttgt aaatgttact
    46261 ggaaagaaac agatttctat tgtttgtttt gtttacaaaa aagataatga aagtgtaaaa
    46321 aagtaaagga gtgagaacat ggcaaaagga atcaaaatca ctacgattgg tggaggcagc
    46381 agttacaccc ccgagttgat ggaaggattt atcaaaagat atgaggaatt accgatacga
    46441 gaaatctggc tcgtagatgt agaagaagga aaaggaaagc ttgagatcgt tagtcagatg
    46501 tctcagcgta tgtgggatgc ttctccgtat gaagtcaaga tctatgcaac tctggatcgg
    46561 gaagaagcat tgaaagatgc ggattttgtt acgactcagt ttcgtgtcgg tcaattagat
    46621 gcacgaatca aagacgagcg gatccctagt tattacggga tgatcggaca agaaacaaat
    46681 ggtgctggcg ggatattcaa agcttttcgt accgttccag tcgttttggc aatcgttgag
    46741 gacatgaagc gtttatgtcc aaatgcttgg ctgatcaatt tcgcgaaccc aagtgggatg
    46801 gtgacagaag cagttatcag atatggcaaa tgggataaag tgattggatt atgcaatgtg
    46861 ccagtgatgg caatgatgac agaaccagca atgcttggca agaaaaaaga agagctgatc
    46921 tacaagtttg ccggtctaaa tcatttccat tggcacaaag tagcagataa taaaggaaat
    46981 gaccgcacgg cagaattgat tgatcgttta tacgatgaag acaatggatt gccgaaaaat
    47041 atctttgata tcccgttttt caaagaacaa ttaaaacaaa tgaacatgat cccttgtggc
    47101 taccaccgct attactatcg tgaagaagaa atgctggaac atgctttaga agaatatcaa
    47161 acgattggta caagagcgca gcaagtgaaa gaaacagaag cagagctatt tgaattatac
    47221 aaagatccag aattggatca taaacctgaa caattgcaaa aacgaggtgg cgcttattat
    47281 tcagacgctg cctgtgaaac gattgcttca atctatggaa ataaagaaac acagatcgtg
    47341 gtatctacaa agaatgaggg agctgttcct gacctgccgg cagattgtgt agtagaagtc
    47401 actgcctatg ttggcgcaca aggagcgaga aatgtagcgt ttggttctct tcctgctgct
    47461 gaaagaggct ggttgcaggt aatgaagaat atggaattgc tgacgatcga agcagctgtg
    47521 actggtgatt atgggacagc tttacaggcg tttacgatca atccacttat acgatcagga
    47581 aagacagcaa aacggattat ggatgagttg tttattgcac accagcagta tttaccgcaa
    47641 ttcaaggaaa caatcagccg attaaaagaa gaaggaatcg aagttcaaga tgaactggca
    47701 agagaattat caagtaatac acatataatg gaggaagtat aatgggattc agaaaagatt
    47761 ttttatgggg cggtgctaca gcagccaatc aatgtgaggg cggttatgat gaaggtggac
    47821 gcggactagc aaacgtagat gttgtgccaa tcggaaaaga ccgttttcct gttatcaccg
    47881 gaaagaaaaa aatgtttgaa tttgatgagg atcattttta tccagcaaaa attgcaatcg
    47941 atatgtatca tcgttataaa gaagatattg ctttgtttgc agaaatgggg ttcaagactt
    48001 atcgaatgtc catcgcctgg agtcgtattt ttccaatggg ggatgagaaa gagccgaatg
    48061 aagctggctt gaaattttat gaggatgtct ttaaagaatg ccaaaagcac ggaatcgagc
    48121 cacttgtgac gattacgcat tttgattgtc caatgcattt gatcaaagaa tacggtgcgt
    48181 ggcgttccag agaactcgtt ggtttttatg aaaatctgtg tcgagtcatc ttcaatcgat
    48241 ataaaggact tgtcaaatac tggctgacat tcaatgagat caatatgatc cttcatgcac
    48301 cattcatggg agcaggactg tactttgaag aaggagaaaa cgaagaacaa gtcaaatatc
    48361 aagcggcaca tcatgagttg gttgcaagtg ccattgcaac gaagattgct catgaagtag
    48421 atccggagaa caaagtaggc tgtatgctgg cagcaggagc atattatcca tacacttgca
    48481 atcctgaaga tgtttgggct tctagaaaat cagatcgaga aaattatttc ttcattgatg
    48541 ttcagtcacg aggagaatac ccagcttatg cattgaaaga aatggaacga aatggcattc
    48601 agatcgaaat ggaaccaggg gatgctgcat tgctgaaaga acatactgtt gattttatct
    48661 ctttttctta ttattcttcc cgagtttcga ctaccgacaa cgaactcttg gaacagactg
    48721 caggaaatat ctttgcttct gtcaaaaatc catatctgca agcaagtgaa tgggggtggc
    48781 aaattgatcc attaggcttg agaataacga tgaatgatat ttatgatcgt tatcaaaaac
    48841 cattatttat tgtagaaaac ggcttaggtg cagtcgatac gccagatgag aatggttatg
    48901 tggaagatga ttaccgaatc gattacctgg cagcgcatat ccaggcgatg aaggatgcag
    48961 tagaactaga cggagtcgat ctattaggct atacgacttg gggatgtatt gatttagtat
    49021 cagctggtac cggtgagatg aagaaaaggt acggattcat ttatgtcgat cgtgataacc
    49081 aaggaaatgg cacattagcc cgttcaaaga aaaaatcatt caactggtat aaaaaagtaa
    49141 ttgattcaaa tggagaaaat ctttcgaacg attgatcaat gatttataaa agtctattgt
    49201 ttttcagcgg atttttcgag tataataaag ctattcgaga aacgcaagca gaacaataga
    49261 ctttttttcg cattggctgt aaagtatgct tacttgatta agtaaaggag aatctacata
    49321 tgtatgatgt aatcgttatt ggcgcaggtc ctgctgggat gactgctgct ttatatgctt
    49381 cacgttcaaa cttatccgta ttgatgatcg aacaaggtgc ccctggcggc caaatgaaca
    49441 atacggcaga aatcgaaaat tatcctggat tcgattcgat catgggcccc gatctagctt
    49501 ataagatgta tgaaagtgtc tcaagatttg ggacagaaaa tgcttatgga atcgttcaag
    49561 atatcaagga tcacggcagc cataaagaag tcatctgtga agataaaagt tacgaaggaa
    49621 aaacagtcat tatcgcaaca ggctgtatcc accgtaaatt aggcgtacct ggcgaggaag
    49681 aatttgctgg acgaggcgtt tcttactgtg cagtctgtga tggtgcgttt ttccgtaaca
    49741 aacgtctagt agtcatcggg ggtggggact cagcagtaga agaagccatc tatttgacac
    49801 aatttgcttc tgaagtagtg atcgtccacc gtagagatga attacgtgca caaaagatca
    49861 tccaagaccg tgcatttgca aatgaaaaaa tttcctttat gtgggataca gtcgttgacg
    49921 aaatcgttgg aaacgaaatg gtagtgacag gtgtgaaagc acgtaatgtc aaaaccggca
    49981 atgaatttga tttagcagca gacggagtat tcatctatgt tggactggat ccattaactg
    50041 caccttttag agaagcaggt ttgacgaacg aagctggctg gattccaaca gacaacgaaa
    50101 tgaaaacgaa tatgccaggc gtttttgcca tcggagatgt tcgtgaaaaa gatttgcgac
    50161 aaatcactac agctgttggt gacggcggta ttgctggaca acaagtgtat aaatacttgg
    50221 agtctttaga agaaacaaca gcataagttt aaaaagagtg aaagaacggt ttcaattgtt
    50281 cttttactct ttttttcgcg tgaaatttac tgaatctgat ttatgcgaac acgtccaaca
    50341 ccttttgata aaagaatcct ttcttgattt aagcgttttt tggtcctcat actttcatat
    50401 ctaataagtg aatgctatac ttattaagaa ccatatttca gaaagaggtg ttattttcat
    50461 gtcttgggaa caagtgtatg aacaatgggt acatgaagaa aatttagaag aaaacttaaa
    50521 aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt gaagatgctt tttatgcacc
    50581 gttggaattc ggtactgcag gcatgcgggg aatcttagga cctgggatca atcgaatgaa
    50641 catctatacg gtaagacaag caacagaagg actagcacgt ttcatggata cacaaggtga
    50701 agaaacaaaa cgccgaggtg tcgctatcgc ttatgactca cgccatcagt caccagaatt
    50761 tgcaatggaa gcagccaaaa cactagcaca tcacaatatc ccgtcttatg tattcgaaag
    50821 tctgcgtcca acaccagagc tttcttttgc agttcgtcat ttgaagacgt ttgcgggaat
    50881 catggtaacc gcatctcaca acccagcggc ttacaatgga tataaagtct acggagaaga
    50941 tggcggacaa atgcctcctg cagacgcaga tgcattgacg aaatatgtcc gtgaagtcag
    51001 caatccgcta aaagtcgaag tattatctga cgaagaagct aaacatagcg ggttgatcac
    51061 gatcatcgga gaagaagtgg acgctgctta tctagaagaa atcaaagcag taacgattga
    51121 tcatgaattg gtggaaacaa tggggaaaga tttgaaactt gtgtacacac cacttcatgg
    51181 aacagggaaa atgttgggtg aacgtgcgtt gaaacaagct ggattcgaac aattcgtact
    51241 agttcccgaa caagctgtgg cagatccaga tttcactacg gtcaaatcgc caaatccaga
    51301 agaacattca gcttttgaat atgctatacg acttggagaa aaagaaggag ctgacttgct
    51361 gattgcaaca gatcctgatg cagaccgttt aggcgcagcg gttcgtttgc cagatggttc
    51421 ttatgaagta ttgactggga accaaatcgg agcattgttt gctcaatata ttttggaagc
    51481 acatcaatca cggggaactt taccagaaaa cgcagcggta ttgaaatcaa tcgtttccag
    51541 cgaattgcct acagcaattg cggaacacta cggtgctact atgttcaatg tgttgacagg
    51601 gtttaaattc attgctgaaa aaatccaaca atttgaagaa gaccattcac atacttttat
    51661 gtttggattc gaagaaagtt atggttattt agttaaacct tttgttcgcg ataaagacgc
    51721 gatccaatca ttgcttttat tagcagaagt agcagcttac tataaaaaac aaggcaaaac
    51781 gttgtatgac ggcttgcaaa cgatttttga aacctatggc tatttcgcag aaaaaacgat
    51841 ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc tctgctttga tggaaaaatt
    51901 ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt attcaaacag aagactttaa
    51961 agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa ctaacaacac cgccatccga
    52021 cgtattgaag tatacattgg aagaaaatgg ctggatcgct atccgccctt caggaactga
    52081 gccgaaaatc aagttttata ttggtgtaaa aggaagctct acagaagacg cagccgaaaa
    52141 agtagcctct tatgaagcga ttatcaaaaa gatgacagaa gaataaaaaa cttgagactg
    52201 atggaaaaag tggtaaactt tagaagtcag tcttttttca agttggaggt gaatggattg
    52261 ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat tattcaaaat gttgagtgat
    52321 cccactcgtc ttaaaattct gctatatctg aaagacgggg aacaaaatgt gacagcaatc
    52381 acccaagcag tggaaatgga gcagtctgct gtatcgcatc aactacgttt attaagagaa
    52441 aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt tgtatagtct tgatgattct
    52501 catgtactgg atattttgaa ccagacactc aaacatgtgg aacatcaata acgataggtg
    52561 caaatagata aacactggag tgtgacaacg aaatttcttc gttgtcacac tccagtgttt
    52621 tttaagaaaa agacaaaaat gattaatcgc tattttctgt tgtttcagtt ccataataac
    52681 tatgatcttc taggtctaaa gcattcaaaa taagcgaagc cgtttcttca atagataaag
    52741 atgccacatt gatcactaca catcctagtt tttcataaag atcattcgca aaatcgagtt
    52801 cagctctaat tttttcgata tcggaataag cagtatcagg gtttaatccg taagagcgca
    52861 tccgttcttt acggatatta tttaaaatat ctgggttatt cgttagtccc acgatttttt
    52921 taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg tgggatcaaa ggtaaattgg
    52981 caacttttaa atttttgtta gctaaaaaca agctcaaagg cgttttggaa gttcgggata
    53041 cgcctagcaa aacgacatct gcttctaaaa aaccacgagg gtctttccca tcatcgtatt
    53101 ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt ctcattcaat aaatggaggg
    53161 caccaggttg ccgaattggc ttgataccag ttaaacgttc aatttctgat atcggtggcg
    53221 tcatcacatc aaaactaaat aattgatgtt cttcacaaaa agaacgtgtt atctcgatca
    53281 gttcttgatt gattaatgta tgcaggacaa gtgctttttc ttctaatgca tcttgcaatg
    53341 cctgccttaa tgttttttca tctgtcacga acgttctgcg gaataggttg aactcaacgg
    53401 taggatactg agccatagta gcttgcgcta gttttgtcgc agtttctcca gcagagtcgg
    53461 agatgacaaa aaaagtaacg atttctgtag aatcggaaga catggataaa acctcacttg
    53521 catttttttt atgtttatta taccatactc tagaatgcgt acttcgtaat cattttgatt
    53581 tggaggaata agtcatgaaa aaacagacat tgatcgaaca aacattagaa acattgaaaa
    53641 ataacggttt taaatatact aaaaaaagag aagcattgtt aacttattta gtgaagcgta
    53701 accgctacgt atctgctaaa gaagtttttg actatatgaa tcaggaattc aaaggggtaa
    53761 gttatgatac gatttatcgg aatctccatg attttgcaga gatcgattta ctggaagaaa
    53821 ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg tcagggggaa atcggccacc
    53881 accatcattt tatctgtaca gtgtgcggaa caacgaaaga aatccatatg tgtccgatga
    53941 attatttcga agaacagcta ggtggatgca cgatcgaagg gcatagattc gagattttcg
    54001 gaagatgtga aaattgtcaa taaggattta aataaaataa catttgataa ttttaaaaaa
    54061 aagcaagatt tccttgacgg tcaaaacagc attagatata atatttacag gacagtcttg
    54121 ttttttgaga tatatttcaa taacaattac ggttactttt taagctcgga gggagggaat
    54181 taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt gacgatgctc ttcgtcgctt
    54241 caaacgttcc gtgtcaaaag cgggtacttt acaagaatct cgtaaacgtg aattctacga
    54301 aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct agaaaacgca aaaaattcta
    54361 gttctgtgat gaattggagt tggtagtgtg tcactactaa gtagattaaa cgatgacatg
    54421 aaagcagcga tgaaagcgaa agataaggaa tctttacaag tcatccgaat gatcaagtca
    54481 tctatacaaa acgagcagat taaagaaggt cacgacttga cagaagaaga agaactaacg
    54541 gtgttgtctc gcgaaatgaa acagcgacgt gattctttgc atgagtttga agaagcagga
    54601 cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa tagtcgaaaa atatatgccg
    54661 gaacaacttt ccgacgaaga aattcgacaa cttgttcaag aagcaatcac tcaaactggt
    54721 gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa ttatgcctaa agtcaaagga
    54781 aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac tattacaatc ttaattttat
    54841 catttaaact gcggacagtg attgtccgca gttttttgtg ttcgtaaaaa aagaactaaa
    54901 aaacagctag aagaaagaat aagattcttt cttctagctg tttttttcct gtttaatatc
    54961 tcttatgtta aattgtattt gatttggtta ttcttctggt aactcacatg cttcttgttt
    55021 atatcctgat acgcgatata cataataaaa gagtaagctg ccagcgtaac caagcaacgt
    55081 tgggacgacc cagccaagac ctagtgcagc aaaaggaaca taatgatgat aaaatcctag
    55141 taagcttgct acgacagttt gctgactcac taatgcaggc gaatttgcca acatatccaa
    55201 tacggctgga acggcagaaa agaacatcgt tacttgatag acaaatggac tcttttggaa
    55261 gaattttgct gttaaggaaa gcaagattaa cgcaagtgac aatggataca aaagcatcaa
    55321 tactggtaaa gaccattgga tgatattatc caatccggca ttcgcaacga caaaagagac
    55381 aaaagtcgtt aaccgcagcc atgtcatata actgacttta gggaacagtt tatggaaatc
    55441 ttgtgcaaaa gaaacaacga gtcccatagc ggtagtgaag acacctaatg tgaccattac
    55501 ccctaagaag ctagcaccga agttacctaa ataaaaatta gtgatctgag aaagtgcagt
    55561 tccgccattt tctgataaag acatcttatt caaactcatc gcaccgagaa ggaccagacc
    55621 aaaataaatc atgacttcta acgcaacgct caggctacca gcttttgctg ttaattctgt
    55681 gactttttta tctttaaaac caagatcacg aactgcatgg acaaaagtga cacttaaagc
    55741 aagtaaagct actgcatcaa ccgtgttgta tccttctaaa acgccatttg tcaaagcgtt
    55801 cgtctgataa gaatctgcag cagattggtt tagattcccc atgggttgga ataaagaaac
    55861 gacaaaaatg atagctaata atgcaagaaa caaggagttt aaatactttc cgacatattt
    55921 gaccaaacgt gttggatgta cagtaaggaa ataagcaagt aaaaagaaaa gagcagagaa
    55981 taaaaacata cctaatgaat catatttttc aggcaaaaaa ggtttagcag ccatttcaaa
    56041 agcagttgct gctgtccgcg gtgtaccaaa gaatggtccg atcgttaaat gaacaaggat
    56101 caagaacaag gcagcatacc attttccgac aggtttagct aagtcataca gaccatcact
    56161 ttttgtcact actacagcta agatagctaa taatgggaaa agagcgcctg agatggcaaa
    56221 tcctacacca gcgctgatcc agtttgatcc agccatttga cctaagtgga caggaaagat
    56281 cagattacct gacccgaaga acatcccgaa gataagtgaa ctgaccatca ataattgctt
    56341 ccatgaataa gtttttgttt cttccataaa tttttcctcc aataattatc attttgagta
    56401 acaaaataaa cgttgcagaa actagaaata ccactaatat cagattccat gaaaaccacc
    56461 accttaaaat ataaaaaaag cccttttcca cttgggaaaa gggctctaga ttctagaacg
    56521 gtaccacctt ttttcgctta aaaaagcttc ttcattcacg gacgcttttc gataaagtta
    56581 tccgctagcc tgataatgga ggcagccaca atgatctacg ttttcgacat tgactcggaa
    56641 atgatgttcg tctattcgat ttattgtcat ttctcactta catgactcac tgggaataaa
    56701 tcactgaata gcctactgtt ttcctcgacg ctttgctgta tatttatttg ttgacgttat
    56761 aatacacctg ttttttttga aatgcaagaa tatttcaaat attttgtttt ctttttttga
    56821 aacaagaaaa atacccagag aatacttgct aagttagacg gaatacttgt cctctatctt
    56881 atgcatagct tacatgatca gcttttttta ttcaaacgag acctttagag aatggaagca
    56941 gctatttttt aacgtttctt ggtacttatg gttaagcaac tcttttagaa ccttttctta
    57001 tttgctatat agtttcttct gagtatggta aaataggaaa gaatgaatcg aaaaaaaggg
    57061 gatgtctatt tttgacggaa gagtctagtt ctttagagat tagattgacg gctgctgatg
    57121 atgtcagcat gcttttaggt gcacacgaca agcatataaa actgattgaa gaaacaacag
    57181 aaacaaccat ccatacacgt ggagaaatca ttcagatcat tggtgaacaa tcccagattt
    57241 ccttggctgc ctcagtcatt cggacattgc aggaattgat caaacgagga atccatgtaa
    57301 gtacaccaga tgtcgtgacg gcactgaaaa tggggcaaaa aggcacatta gattattttg
    57361 ttgagatgta tgaggaagag atcgtcaaag accgtaatgg aaaacctata cgggtaaaaa
    57421 acagcggcca aaagaaatat gtcgaatcgg tagccaaaca tgatatcgta tttggcgtag
    57481 gaccagccgg gacaggaaaa acttttcttg cagtagtatt agcaatcgct gccttgaaaa
    57541 aaggtgaagt gcaaaaaatc attttgacgc ggcctgctgt ggaagcaggg gaaaatctag
    57601 gctttcttcc cggggatcta aaagaaaaag tagatcctta tctgcgtcct gtttatgatg
    57661 ctttatacca aatttttgga ttagatcata cgaaccgatt gatggaacga ggcgtgatcg
    57721 aaattgctcc tttagcttac atgcgggggc ggacattgga tgatgcattc gttattttgg
    57781 atgaagcaca gaacacgaca gttgctcaga tgaaaatgtt cctgacacgt cttggttatc
    57841 aatcaaaaat gattgtcaat ggagatacga gtcagattga tctgcctcga gggacaacaa
    57901 gtggtcttgt tcatgcagaa cgaacattaa agcaaatcaa gaaaatcgat tttgttaatt
    57961 ttgaagcaag tgacgttgtt cggcaccctg tagttgccga aatcatcaaa gcttatgaaa
    58021 aggcagatct ccatcaagaa taagctttgc ttaggaggat aagcacatgt tgaataaaac
    58081 actgcagaaa ctacaagaaa aacttggaaa ggcagcattg cctttcctgc tgttcttgtt
    58141 ttttatcatt atctgcggcg ttacatttag tagtgtcaaa caaaaggcca atgatttcag
    58201 agaaggccaa gtagcagaag aaagtatccg tgcaaacaaa acgatcgaga acacggaaga
    58261 aaccgaacaa aaaagaaaac tggcagcaga agcagtcact ccagaataca cttaccaaaa
    58321 agatcttgca gatgaccaga acaatcggat caagcaacta tttgaattga tcgataagac
    58381 gaatgatgcc atcaacaaat cttatgacga aaagattgat aaggcaaagg aagatgaaaa
    58441 tatcccaaag cctactacag aagagcggat cgccgcgctg aagaaaaatt ttgaaaatgt
    58501 gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac aatttttatt cgacgatatt
    58561 tgaaatgact gaaactgaga tccatactgt gagggatgag agtctgaagc tcattgacga
    58621 acagatgagc aaacaagtac gtgaaagcga attggaagct tttaaacaag aagcagaaga
    58681 gcaaatccaa tatttgaatg tcacaccagc acaacaacaa atgattcgct acttagtgga
    58741 tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag acagaagaat tgaaacaatc
    58801 cgcacgagaa gctgtacaac cagtgatgat cttccaagga gaaatcattg ttcgtgaagg
    58861 taatcaaatc gatgcttcag ctatgaaaaa attggaatta ctaggactga ctaatcaaac
    58921 aacttcgatt tttccgctaa ttgccatggt tttagctgtt ttgcttcaaa tcggcgtact
    58981 gatttataat tcacttcaat tccaaaatgc aggagaaaga acaaaatacg tgcttttcta
    59041 tgtgacagct atgtcagtca gcatagtgct gatgaaattt ttccaattat tccaaacgga
    59101 acaagctact tatatcccgc tgttttatcc cgctgctttt gcaccacttg ttttaagctt
    59161 tttcttgaat cgaagagcag ggatcatggc tggtattttc caagcagtat ctgcactttt
    59221 cattttttat gattcgattg gcacgaactt tctaacgatt atcttgatgt cttatgtttt
    59281 ctctgggctc atggcaacga ttgtcagacg taaacggatc tcagaacaag gattatctgc
    59341 tgccatgtgg gtcattattt ttcctgtgat gatggatgtc attttgatcg tctatcaagg
    59401 aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc tgcgggttca ctggtgcact
    59461 cttctcattc ttgctgacga tcggtctaca cccatatatt gaattgacgg tcaatgatga
    59521 cagtgtaatt gtattaaatg aactcagcaa tccaaatcat cctttattga aacagttgct
    59581 ggaagaagcg ccaggtactt atcatcatag tatgatggta gctaatctaa gtgctaacgc
    59641 tgtagcagaa atcggcggac gttctttgct aactcgagta gcttgctatt atcatgatat
    59701 cggcaagatc aaacatgcta gctttttcgt tgagaacttg ccttcaggag cggaaaaccc
    59761 gcacaatttc ttattgccag aagacagtaa acatattatc tttggacatg tgacagatgg
    59821 agccaaaata ttggaagatt ataatatgcc gcaaatggtg atcgatattt gcaaacagca
    59881 tcatggaacc acgctaatgc gttatttcta catcaaagcg aaagaacgca atccggaagt
    59941 aacagaagag caattccgtt accctggacc aaaaccgcaa agtcgcgaag cagggatcgt
    60001 caatattgct gacagttgtg aggctgcagt acgggcaatg gatcatccga cgagcgaaaa
    60061 aatcgaaagt tttgtccata atcttatcga agagcgtatt tccgatggtc aattagacga
    60121 tagtggactg accttgaaag aaatccgaaa agtggaaaaa tcattgatca gcggtttaag
    60181 tagcaccttc cattctcgga tcaaatatcc taaaatgaaa acagaagttg agaagataaa
    60241 ggaagaacaa gaagaataga aagaagcgat gtataatgga tattaccttt atggatgaaa
    60301 caaatcaagt ctcagaagag aaaatgaaag aaatcgatga cttgctgcaa tttgcagcat
    60361 cttatttaaa gctgccagag gaaacagaaa tgtctgttac tttcatggac aatgcggcaa
    60421 tccaagtaat caatcgtgat taccgtggaa aagatgcacc gacagacgtc atcagttttg
    60481 ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt tgaagatgga gagaatccat
    60541 tgcctcgtgt actaggagac atcatgatct ctattgaacg tgctgctgaa caagcagaag
    60601 cttacggcca ttcatatgat cgggaacttg gctttttagc ggtacatgga ttcttgcata
    60661 tcaacgggta tgaccatatg acaccagaag atgagaaaga aatgtttggt ttacagaaag
    60721 agatcttgga tgcctatgga ctcaaaagat aaaaacgtag agaaaaacaa acatttcctg
    60781 atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt ttgaggaaga acgcaatatg
    60841 aggaaacatg ttgcgttcgg tatccttgct ttatttatgg gcgtgatttt ccagcttgat
    60901 cggatagagt ggttatggct gcttttagct gtttttctcg tttggatagt agagatcatc
    60961 aatactgttt ttgaaaatgt agtggacatg tttacggatt ttcattttca tccgattggg
    61021 aaaaaaatca aagatatggc agctggagca gttttagtga ccgcttgttt tgctgtcatc
    61081 attgggttga ttttatttgt ccctaaaata tatcatttgt tgtttaatta aaaaggagaa
    61141 cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt cggccgcccg aatgtaggaa
    61201 aatccacatt attgaaccga atcgtgggtc aaaaaattgc cattatgagt gacaaggcac
    61261 agacaactag aaataaaatc caaggagtat atactacacc agaagctcag ctgattttta
    61321 tcgatacacc aggaatccat aaaccaaagc atcgtctagg agattttatg gtagaagcag
    61381 cttacagtgc gttgaaagaa gtggatgcag tcttatttat gatcagtgct gatcaaaaac
    61441 gcggccgggg agacgatttt attattgaac gtctaaaaaa cgtgcattct cctgtttatc
    61501 tgattatcaa taaaatcgat aagatccatc cagacgagtt gctgggaatc atcgaagatt
    61561 attcgacgca gatgcctttt gcacaagtga ttccgatctc tgctacagaa ggaaacaatg
    61621 tcgaacgctt aatggatgta ttagtcagtg aaatgccaga gggcccacaa tattttccag
    61681 atgatcaagt gactgatcat cctgaatact tcatcgtttc tgaattgatc cgtgagaaag
    61741 tcttgttttt aacacgagac gaagtgcctc attctgtcgc agtcgttgtt gattcgatga
    61801 agcgaaacga gaatgataaa atccatatcc aggccacgat catcgtcgaa agagatagcc
    61861 aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa gcaagtagga acgaaagcaa
    61921 gaaaggatat cgaaaatctt ttaggagaca aagtatttct agaactttgg gtcaaagtcc
    61981 aaaaagactg gcgagacaaa aaagtctatc tgcaagactt tggctaccga aaagacgaat
    62041 actgatattc tgaacttctt ataggttaag tatagaaata aactggataa aaatccgcac
    62101 gttgagaaaa acagcggaag atcgtaacat aaataatctg gatcggcaga atctgctttt
    62161 ccagatttat tttttaggag ggagaatatg agccagcttg cagaatcgaa aggcttgatt
    62221 ttatctagcc gtaattacaa agaaaaagat aaattagtca aaatctttac ggaatcttct
    62281 ggcaaaatga tgttttatat caaaggaatc catcggcaaa atcagccatt agctccggct
    62341 ttgctcccct ttaccgaagc agtctatatc ggtaattttc gaagtgaagg tttgtcgttt
    62401 ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc aagaagatat ctttttgagc
    62461 ggttatggta cgtatttgct aaatctagta gatgcagcaa tcgaagatca tcaatatgac
    62521 ccgcatttat tccaatttac gcaacaagct ctgcagcgga tggaccaagg agatgatgca
    62581 gaaatcatca cgaatatctt tgaagttcaa atactgcaac gcttcggtat cgcgccaatt
    62641 tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat ttgactattc ttccaaatat
    62701 ggcggtgtga tatgcgagaa acactggcca atggacgaac accgttatca tgcagatcct
    62761 cgcgctgttt atttcgttcg aatgttttcg gctatttctt atgataagat cagtggaatc
    62821 cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca ttgaccagtt gtatgatgaa
    62881 tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc aaatgaagtc ttgggcacac
    62941 gtattaaaga cgccagagaa aaatatgcct gaaaaagaga tacctgaaaa agaagaataa
    63001 atgaaagata caacaaaaaa atcaaaacta tttgacaaat acagggactt ccgtctatta
    63061 ttaaccgtag attaaataga tcgttcactg agaggatcta ttatacaaac aagaaaactg
    63121 caaaatgaat acgtacattg accaagatca gtaaaaaaca cgtggtttag cgagttcggg
    63181 agagtggaag ccggacccaa acgatttttg aagaggcact tgcgagtacg ataaatgaag
    63241 ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa ttattcgtcc ctatgtctgt
    63301 aatcagacat agggactttt tgtcgttttc tttcctgtat tcttattgac agcaacgctt
    63361 aaaaattgaa aggggcaaaa cagatgagta aaaaattaac cgtccaagat atgattttaa
    63421 cattacaaaa attttggtca tcaaacggct gcatgctgat gcaagcttat gatacagaaa
    63481 aaggagcagg tacgatgagc ccatatacat tcttacgggc aatcggaccg gaaccatgga
    63541 atgctgctta tgtagagcca tcccgccgac cagctgacgg gcgttatgga gaaaacccaa
    63601 accgtttgta tcagcatcat caatttcaag tcgtaatgaa accttcacct gaaaatatcc
    63661 aagagcttta tttagaaagc ttgcgtcttt taggaattga tccattagaa cacgatattc
    63721 gttttgttga agacaactgg gagaacccat cgatggggtg tgccggtgtg ggttgggaag
    63781 tatggcttga tgggatggag atcacacaat tcacgtactt ccagcaagtc ggaggactac
    63841 cttgtaaacc agttacttct gagatcactt atggactaga acgtcttgct tcatatattc
    63901 aagaagttga aagtgtctat gatctggaat gggctgatgg tgtgaaatat ggcgagatct
    63961 tcaaacaacc agaatatgaa cattccaaat attcttttga agtaagtgac caagacttgc
    64021 tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg ctgtatcgat gaaaatcttg
    64081 ttcatccagc gtatgattat attttgaaat gcagccatac attcaattta ttagatgctc
    64141 gcggtgcagt atccgtaaca gaacgagcag gttatctttc ccgtatccgc aacatggctc
    64201 gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt aggattccct ttattgaata
    64261 aaaacacagc aaattcagaa aacatgaagg aggctgaata agatggcaaa agacttatta
    64321 cttgaaatcg ggctggaaga aatgcctgct catgttgtca caccaagcat gaaacaatta
    64381 gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga cttatgatac gatcgaaacg
    64441 ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca ttccagaacg tcaagcagac
    64501 agtgaagaag aagtcaaagg accagccaaa aaaattgctt tggacgcaga tggaaactgg
    64561 acgaaagcag ctgaaggatt tgtccgcgga caaggtctga cgacagatgc tatcactttt
    64621 agagaattaa aaggcgtgga atatgtctat gtaacgaaat acactcacgg caaagaagcg
    64681 aaagaagtat tgacaggttt agcagatgtc attaaaagcc tcactttccc agtaacgatg
    64741 cattgggcag aatacgattt tgaatatatc cgccctattc actggatcgt ggcattgtta
    64801 gatgacgaaa taatcccgtt tgagatcttg gatgtcaaaa cagacagagc ttcaagaggt
    64861 catcgttttt taggcgaaga tgtgacattt acacatgcaa aagaatacga agaaaaatta
    64921 aatgaacaat ttgttattgc ttcaccgcaa aaaagaaaag aaatgatcat cacacaagca
    64981 aacgaattag cagaaaaaaa tcactggatc attgatctag acgaagattt actagaagaa
    65041 gtcaacaatc tagtcgaata tccaactgtc ttttctggcg acttcgagga aaaatatctt
    65101 tctgttcctg aagaagtact agtcacttct atgaaagaac atcaacgtta tttcgaagta
    65161 cggaacgatc aaggtatgtt gcttcctcat tttatttctg ctcgtaatgg cgatcgggta
    65221 aaattggaaa atgtggtacg aggcaatcaa aaagtattga ctgctcgttt agaagatgcg
    65281 gaattctttt ataatgaaga caagaaagct tcaatcgacc aatacgttga aaaattaaaa
    65341 actgttactt tccatgaaaa aatcggtagc atctacgaga aaatgcaacg ggtacaagtc
    65401 atcgctcgca tcataggcga acaagtagga ctatctgagg aagaattgat ggatctgcaa
    65461 cgcgctgctg aaatctataa atttgatcta gtaaccaaca tggtaggaga attccctgaa
    65521 ctacaaggga tcatgggaga aaaatatgct ctgcttaaag gcgagaaacc agccgtagca
    65581 acagcgatcc gtgaacatta catgcctata tccagtgaag gagatctacc tgagactaca
    65641 gtaggttctg ttttagcgat tgctgataaa ctagatagta tcttttcttt ctttgcagtt
    65701 gggatgatcc caagtggctc aaacgatcca tatgctttgc gccgtcaagc atatggtgtc
    65761 gttcgagtca tcgaatcgaa aaaatgggcc tttcctttgt ctgttctgca agaaacgatt
    65821 agtgaagtta tcagtaaaga tacggatcgt tttggcattg gcttgtcagc gggacaacaa
    65881 caggtgatcg actttatcaa aggccgtctg cgccaattat tgacaacgaa gaatattcgt
    65941 catgatgtca tcgaagctgt tttgaacgca gaacaaaaag atctgacaaa agtatttgca
    66001 gcagcacaat tgttcaagca gcatcttgca gatgaagact tcaaaccatc gatggaagct
    66061 ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg aaaaacaatc agaagtagac
    66121 cctgaactgt tcgaaaacga agcggagaaa gaactgcaca aagcagtaga ggcaatgaaa
    66181 acaagctttg ctgaccaaac gatcaacgaa aattatgatc agcttgtaag ccttcgccca
    66241 ctgatcgaaa attattttga tcagacgatg gtcatggcag aagaaccagc agtacgtaag
    66301 aaccgattaa cgcaattaag tcaaattgct gccatggctt tgtcactagc aagtttagat
    66361 caaatcatca caaaataatt tctaatatgt aaataaacca gagaacaggc acaaaatagg
    66421 ctat
//
LOCUS       DAGCXG010000006        64300 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGCXG010000006 DAGCXG010000000
VERSION     DAGCXG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.006"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(531..2027)
                     /gene="lysS"
                     /locus_tag="MB022_000385"
     CDS             complement(531..2027)
                     /gene="lysS"
                     /locus_tag="MB022_000385"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBT4465413.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="MB022_000386"
     CDS             complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="MB022_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBT4465414.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="MB022_000387"
     CDS             complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="MB022_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBT4465415.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4247..6358)
                     /gene="ftsH"
                     /locus_tag="MB022_000388"
     CDS             complement(4247..6358)
                     /gene="ftsH"
                     /locus_tag="MB022_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBT4465416.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="MB022_000389"
     CDS             complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="MB022_000389"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBT4465417.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="MB022_000390"
     CDS             complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="MB022_000390"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBT4465418.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8558..9028)
                     /locus_tag="MB022_000391"
     CDS             complement(8558..9028)
                     /locus_tag="MB022_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBT4465419.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9074..9499)
                     /locus_tag="MB022_000392"
     CDS             complement(9074..9499)
                     /locus_tag="MB022_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBT4465420.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9566..9835)
                     /locus_tag="MB022_000393"
     CDS             complement(9566..9835)
                     /locus_tag="MB022_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBT4465421.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9846..11441)
                     /locus_tag="MB022_000394"
     CDS             complement(9846..11441)
                     /locus_tag="MB022_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4465422.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="MB022_000395"
     CDS             complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="MB022_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBT4465423.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14994..15554)
                     /locus_tag="MB022_000396"
     CDS             complement(14994..15554)
                     /locus_tag="MB022_000396"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBT4465424.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15907..16881
                     /locus_tag="MB022_000397"
     CDS             15907..16881
                     /locus_tag="MB022_000397"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4465425.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17051..18451
                     /locus_tag="MB022_000398"
     CDS             17051..18451
                     /locus_tag="MB022_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBT4465426.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18529..19251)
                     /locus_tag="MB022_000399"
     CDS             complement(18529..19251)
                     /locus_tag="MB022_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4465427.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19330..19836)
                     /locus_tag="MB022_000400"
     CDS             complement(19330..19836)
                     /locus_tag="MB022_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4465428.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19959..21758
                     /locus_tag="MB022_000401"
     CDS             19959..21758
                     /locus_tag="MB022_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBT4465429.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21826..23400)
                     /locus_tag="MB022_000402"
     CDS             complement(21826..23400)
                     /locus_tag="MB022_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4465430.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23655..24164)
                     /gene="mreD"
                     /locus_tag="MB022_000403"
     CDS             complement(23655..24164)
                     /gene="mreD"
                     /locus_tag="MB022_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBT4465431.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24168..25022)
                     /gene="mreC"
                     /locus_tag="MB022_000404"
     CDS             complement(24168..25022)
                     /gene="mreC"
                     /locus_tag="MB022_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBT4465432.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25177..25815)
                     /locus_tag="MB022_000405"
     CDS             complement(25177..25815)
                     /locus_tag="MB022_000405"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBT4465433.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25816..26466)
                     /locus_tag="MB022_000406"
     CDS             complement(25816..26466)
                     /locus_tag="MB022_000406"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBT4465434.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26479..27378)
                     /gene="rsgA"
                     /locus_tag="MB022_000407"
     CDS             complement(26479..27378)
                     /gene="rsgA"
                     /locus_tag="MB022_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBT4465435.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27543..29612)
                     /gene="pknB"
                     /locus_tag="MB022_000408"
     CDS             complement(27543..29612)
                     /gene="pknB"
                     /locus_tag="MB022_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBT4465436.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29609..30349)
                     /locus_tag="MB022_000409"
     CDS             complement(29609..30349)
                     /locus_tag="MB022_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBT4465437.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30362..31720)
                     /gene="rsmB"
                     /locus_tag="MB022_000410"
     CDS             complement(30362..31720)
                     /gene="rsmB"
                     /locus_tag="MB022_000410"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBT4465438.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31720..32658)
                     /locus_tag="MB022_000411"
     CDS             complement(31720..32658)
                     /locus_tag="MB022_000411"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBT4465439.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32651..33142)
                     /gene="def"
                     /locus_tag="MB022_000412"
     CDS             complement(32651..33142)
                     /gene="def"
                     /locus_tag="MB022_000412"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4465440.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33157..35565)
                     /gene="priA"
                     /locus_tag="MB022_000413"
     CDS             complement(33157..35565)
                     /gene="priA"
                     /locus_tag="MB022_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBT4465441.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35818..36123)
                     /locus_tag="MB022_000414"
     CDS             complement(35818..36123)
                     /locus_tag="MB022_000414"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBT4465442.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36128..36742)
                     /gene="gmk"
                     /locus_tag="MB022_000415"
     CDS             complement(36128..36742)
                     /gene="gmk"
                     /locus_tag="MB022_000415"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBT4465443.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37229..37513)
                     /locus_tag="MB022_000416"
     CDS             complement(37229..37513)
                     /locus_tag="MB022_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465444.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37531..38421)
                     /locus_tag="MB022_000417"
     CDS             complement(37531..38421)
                     /locus_tag="MB022_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBT4465445.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38441..38659)
                     /locus_tag="MB022_000418"
     CDS             complement(38441..38659)
                     /locus_tag="MB022_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBT4465446.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38629..40329)
                     /locus_tag="MB022_000419"
     CDS             complement(38629..40329)
                     /locus_tag="MB022_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBT4465447.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40596..41369
                     /locus_tag="MB022_000420"
     CDS             40596..41369
                     /locus_tag="MB022_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465448.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41450..42019)
                     /gene="thiT"
                     /locus_tag="MB022_000421"
     CDS             complement(41450..42019)
                     /gene="thiT"
                     /locus_tag="MB022_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBT4465449.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(42085..42187)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(42193..43164)
                     /locus_tag="MB022_000422"
     CDS             complement(42193..43164)
                     /locus_tag="MB022_000422"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4465450.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43258..44631)
                     /gene="glmU"
                     /locus_tag="MB022_000423"
     CDS             complement(43258..44631)
                     /gene="glmU"
                     /locus_tag="MB022_000423"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBT4465451.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(45134..45952)
                     /gene="purR"
                     /locus_tag="MB022_000424"
     CDS             complement(45134..45952)
                     /gene="purR"
                     /locus_tag="MB022_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBT4465452.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(46119..46943)
                     /locus_tag="MB022_000425"
     CDS             complement(46119..46943)
                     /locus_tag="MB022_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4465453.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46916..47596)
                     /locus_tag="MB022_000426"
     CDS             complement(46916..47596)
                     /locus_tag="MB022_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4465454.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47619..48557)
                     /locus_tag="MB022_000427"
     CDS             complement(47619..48557)
                     /locus_tag="MB022_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBT4465455.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48802..49665)
                     /locus_tag="MB022_000428"
     CDS             complement(48802..49665)
                     /locus_tag="MB022_000428"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBT4465456.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49807..52380)
                     /gene="mprF"
                     /locus_tag="MB022_000429"
     CDS             complement(49807..52380)
                     /gene="mprF"
                     /locus_tag="MB022_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4465457.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52521..53327
                     /gene="proC"
                     /locus_tag="MB022_000430"
     CDS             52521..53327
                     /gene="proC"
                     /locus_tag="MB022_000430"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBT4465458.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53406..54962)
                     /gene="rny"
                     /locus_tag="MB022_000431"
     CDS             complement(53406..54962)
                     /gene="rny"
                     /locus_tag="MB022_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBT4465459.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55373..56422)
                     /gene="recA"
                     /locus_tag="MB022_000432"
     CDS             complement(55373..56422)
                     /gene="recA"
                     /locus_tag="MB022_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBT4465460.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56531..57772)
                     /locus_tag="MB022_000433"
     CDS             complement(56531..57772)
                     /locus_tag="MB022_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBT4465461.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(58012..58590)
                     /gene="pgsA"
                     /locus_tag="MB022_000434"
     CDS             complement(58012..58590)
                     /gene="pgsA"
                     /locus_tag="MB022_000434"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBT4465462.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58799..59722)
                     /locus_tag="MB022_000435"
     CDS             complement(58799..59722)
                     /locus_tag="MB022_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465463.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(60461..61747)
                     /locus_tag="MB022_000436"
     CDS             complement(60461..61747)
                     /locus_tag="MB022_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4465464.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61740..63008)
                     /locus_tag="MB022_000437"
     CDS             complement(61740..63008)
                     /locus_tag="MB022_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4465465.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            63260..63721
                     /gene="mscL"
                     /locus_tag="MB022_000438"
     CDS             63260..63721
                     /gene="mscL"
                     /locus_tag="MB022_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBT4465466.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63780..64226)
                     /locus_tag="MB022_000439"
     CDS             complement(63780..64226)
                     /locus_tag="MB022_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4465467.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT        17756 a        13942 c        11494 g        21108 t
ORIGIN      
        1 agagtgtttc ccgattcatt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcgg
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
      301 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      361 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      421 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      481 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      541 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      601 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      661 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      721 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      781 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      841 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      901 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      961 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
     1021 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
     1081 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
     1141 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
     1201 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1261 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1321 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1381 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1441 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1501 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1561 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1621 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1681 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1741 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1801 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1861 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1921 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1981 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     2041 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     2101 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     2161 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     2221 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2281 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2341 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2401 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2461 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2521 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2581 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2641 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2701 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2761 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2821 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2881 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2941 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     3001 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     3061 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     3121 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     3181 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3241 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3301 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3361 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3421 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3481 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3541 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3601 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3661 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3721 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3781 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3841 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3901 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3961 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     4021 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     4081 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     4141 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     4201 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4261 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4321 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4381 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4441 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4501 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4561 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4621 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4681 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4741 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4801 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4861 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4921 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4981 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     5041 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     5101 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     5161 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     5221 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5281 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5341 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5401 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5461 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5521 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5581 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5641 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5701 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5761 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5821 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5881 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5941 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     6001 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     6061 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     6121 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     6181 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6241 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6301 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6361 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6421 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6481 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6541 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6601 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6661 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6721 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6781 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6841 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6901 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6961 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     7021 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     7081 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     7141 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     7201 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7261 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7321 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7381 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7441 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7501 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7561 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7621 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7681 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7741 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7801 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7861 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7921 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7981 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     8041 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     8101 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     8161 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     8221 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8281 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8341 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8401 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8461 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8521 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8581 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8641 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8701 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8761 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8821 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8881 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8941 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     9001 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     9061 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     9121 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     9181 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9241 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9301 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9361 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9421 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9481 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9541 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9601 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9661 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9721 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9781 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9841 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9901 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9961 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
    10021 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
    10081 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
    10141 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
    10201 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10261 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10321 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10381 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10441 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10501 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10561 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10621 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10681 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10741 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10801 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10861 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10921 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10981 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    11041 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    11101 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    11161 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    11221 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11281 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11341 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11401 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11461 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11521 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11581 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11641 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11701 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11761 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11821 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11881 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11941 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    12001 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    12061 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    12121 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    12181 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12241 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12301 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12361 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12421 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12481 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12541 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12601 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12661 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12721 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12781 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12841 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12901 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12961 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    13021 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    13081 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    13141 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    13201 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13261 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13321 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13381 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13441 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13501 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13561 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13621 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13681 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13741 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13801 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13861 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13921 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13981 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    14041 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    14101 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    14161 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    14221 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14281 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14341 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14401 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14461 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14521 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14581 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14641 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14701 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14761 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14821 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14881 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14941 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    15001 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    15061 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    15121 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    15181 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15241 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15301 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15361 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15421 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15481 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15541 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15601 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15661 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15721 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15781 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15841 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15901 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15961 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    16021 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    16081 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    16141 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    16201 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16261 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16321 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16381 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16441 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16501 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16561 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16621 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16681 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16741 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16801 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16861 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16921 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16981 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    17041 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    17101 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    17161 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    17221 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17281 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17341 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17401 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17461 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17521 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17581 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17641 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17701 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17761 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17821 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17881 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17941 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    18001 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    18061 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    18121 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    18181 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18241 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18301 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18361 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18421 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18481 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18541 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18601 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18661 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18721 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18781 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18841 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18901 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18961 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    19021 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    19081 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    19141 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    19201 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19261 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19321 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19381 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19441 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19501 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19561 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19621 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19681 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19741 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19801 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19861 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19921 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19981 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    20041 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    20101 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    20161 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    20221 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20281 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20341 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20401 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20461 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20521 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20581 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20641 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20701 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20761 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20821 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20881 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20941 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    21001 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    21061 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    21121 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    21181 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta
    21241 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21301 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21361 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21421 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21481 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21541 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21601 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21661 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21721 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21781 aaagagttag tccagaatgg gctaactctt tttgattaat cattcttaca tgctgacagc
    21841 aaagtcaggg gcaaaccatt gaactgaacc aacttttacg ctttcgcctg gttgaggagc
    21901 atggatatat tgtcctccgc ctaaagcaat cgctacgtgg taagttccgc cttgtgaccc
    21961 ccagaataac aagtctccag cttttgcttg agaaactgaa atttttgttc cagctgattc
    22021 ttgaggaact gtccaaccac cgatgtcacg accagttact tgcatgtaaa catagcgtgt
    22081 gaaccctgag cagtcaaagc cacttggatc tttaccgccc caaacatatg gagtaccaat
    22141 atatttgtaa gcttctgcta cgattgctgc accattcaca cttccagaag gagctggagc
    22201 aggtgttaca ggttttggtt gttcaggtgt tggtgttggc gttggagccg gagtactact
    22261 tcctgtgccg tttcctgtat caacactttg atctgttgat ggtgttgtag gcgcaggtgt
    22321 tgttgattct tctgttgttg aagattcagg cactgttgtt gattcttctg ttgctgaaga
    22381 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcgctgttg ttgattcttc
    22441 tgttgctgaa gattcaggcg ctgttgattc ttctgttgaa gattcaggtg ttgttgattc
    22501 ttctgttgct gaagattgtg tagctgatga tgattcttca gtcattgaag attgtgtagc
    22561 tgatgattct tctgttgctg agctttgtgc agtagatgaa gcttgagtag cttgtgcttc
    22621 agcctctgct tgttcgcgtg cttctttttc agctttttct tgtgcagctt gttgacgagc
    22681 ttgttctgct agacgttgtt gttcacgaat acgagcttgc tctgcttcag cttctgcttt
    22741 ttgacggttc aagtcagctt tcttgtcttc agcagttgct tgttcagcag ctaatgaagt
    22801 tttcagtacg ttcaaatcag cttgtttaga taaaagatcg cctttttgag attccaaagc
    22861 agcttggttt tcagctaatt cttttagttt agcatcgttt tcagcttttt tgtcttctac
    22921 agctttttta tcttgttttt gttgttccat caagtcgttg tttgctttta ccatcgttgt
    22981 cattgcttga acgcgtccaa tagcatctgc taatgaatca gcattcaata cagcatcgat
    23041 gtagtttgag cttgtgttgc ttacttgtgc ttcgcgtgct tgtttttgga ttgtatcttc
    23101 acgtttttca atacgttctt gtaaatctgc aatgtctttt actaattgtg cagattcttg
    23161 acgcaatgta tcttgttttg ctaataggtc ttgtgcttgt gtgttgattt cagaaacttg
    23221 gctttctaag gcatcgattt gtgattgtgc atctgcttgt tgatttttca agtctgcaat
    23281 cttttgatct tgctgttgta tctgagaatc aaaatcgtcc gccgcggcag cgatcggtga
    23341 tgctacggca gtcaaggtca ttgaacatac cattactgct gatattaaac tctttttcac
    23401 tcttcattcc tccgactggc ttaatttaat aaatatctaa atatatttaa gtctttctta
    23461 acgatataaa aattgtacca tagcagtgtg tcaacgatat aatagttatg ttacaaaaat
    23521 gtttcaaaac aacttttaaa aatgacaaaa ctgttcgttt cagaaaatga tcatgaaaaa
    23581 gccgcgttaa gtctagcatt tcttaagata acaacgattc tttttcgctt tcgacctaaa
    23641 aataaaaaaa gaaattattc atcactgaat aatttcttaa agggaaaaat gaataataca
    23701 aatataatca tgttcaacag cagtgtaggt cctaaaaacc ttgtaataaa atacgtgtta
    23761 tttactactg ctaatttaaa aatcaactgg accaccatcg taaacaattc ataacccgtg
    23821 acaaagatga tcatactgaa aaattcagta aagatgttga catggataac gtctttcatt
    23881 acatacatca accatacaat taaagggaga gctactgcat agataccgat aacgccaata
    23941 tagtaagcgt catagatagc cccgagaaca atcgtggtaa tcagtaaata acgtttttca
    24001 aacgccatac tgcaacataa taatgccaat atcagaaagt gggcatttga catataggtg
    24061 cctttcgacc actcccctag cattcttgtt aaatgaccat ctatcaacat caaaaagaac
    24121 aaaacgattg gcagatagta cttcattgtt tctttcttca acatggacta atcctccgct
    24181 agccgttgga caatcgtcac aactgacacg tcatacattt ctgcataagg tttcacataa
    24241 acttcccgat ccagcccata actatctggt ttcgttttaa taaccgtacc gatcggcaag
    24301 ttagctggag aattccctcc aagaccggat gtctggacaa catccccttc tttgatatcc
    24361 gtatcaccag ttaattgggt caccactaag gcatggagct tttcatcata gtttttaagc
    24421 aaaccaaacg cttcgccatt agccgaagat acccgtactg gaaaatgatt ggagctttca
    24481 ttagaggatg acagtaattc gattttagac gaagccgtat tgacctcgat cactcggcca
    24541 atcaatcctt tttgcgacat gactgccata tttgcttcga taccgtcttt agaacccttg
    24601 tctacgatca gcatgtcctg ccaactgtca ggcgaacgag taatgacatt tgccgttact
    24661 ttttcgtaac tagtcagcgt ctgattcaac tcaagttctt ttttcaacgc ttcgatttct
    24721 ctctcgtaat tcttgttttg ctgtgaaacg tcatcatagc tatcgatttt ttcttttagc
    24781 cgctcatttt cggcataagt tgaaaaaaga ttatggacag aatctactcc atttcgtacc
    24841 caccgtactg gggcagagat tgttttatca accattgcaa cactgtcatt aacgatggat
    24901 tggaagaaat tgggctttcc ttcattcgct cgctgcgcag cggtcacact tagtatcgtc
    24961 accacgatga taacaataac caaggtaata ataatatttt tattaggatt gaacttcttc
    25021 acaaagacac ctcgaattaa ttagactctt gtgttcaatt ttatcactaa atgccgtgaa
    25081 acaaaaggta agttgctcct cttaagcttt tttttagcaa ataatagatt ctgtcatttt
    25141 tagcctgaaa gacaaatttt tttatttgtt tatggatcaa tctttgcttt gaataactgc
    25201 aatcatacca tctgaaaaag aaaattctgc ggtatctgaa agaaattcgt tacttgcata
    25261 agatgtcgga tattctactt ctttattttc caatacatac ttgctgcctt ttaatgtaag
    25321 attcgagacg ggagtcaagc agcaataagc taaatacttc atatctggta tctgtcttac
    25381 tatatgctct ccaggcaaat aataagacaa ataattttga caatctctga taataagctg
    25441 atgggcaaag ggtttgaagc gaggttccat acctaaccag agattggcta aaagatgatc
    25501 caatcttccg cccgtagctc caatcaaagt gacttccgct tgcgggtaac gttcaaatgt
    25561 ttttaagata cctaactgtg tatctgtatc gtctttttct gcaggggaac gggtatactc
    25621 tttggctttt gcaaatactt ccttctgctc ttcgttcgac aatgaatcaa aatcacccac
    25681 tgccagatct actgggtagc ctgcttgaag caggtacagc gcaccacggt ctatcccgac
    25741 ataggcatcg tatttggaca gcagagactg cggccagttt tgcggattac ctccagcagc
    25801 taataaaatt ttcatttagt ctaatgcctc ttttaatcgt tcgatttgtc cgactggatt
    25861 ttcggctcca tagatatatg atcctgcaac gaagacatcc gcccctgcat ctttacagat
    25921 ttttgctgtt tctggaacga ttccgccatc tacttcgatt gcataagtcc aattgttctc
    25981 tctacgtaga tcagcgagtt ccgtaatttt ttctactgtt tcttcaataa agctttgtcc
    26041 gccgaaccca ggattgacag tcatgactag tacttgatca gccaaaggca aaacatgttt
    26101 gatcatggag actggtgttc ctggattgat aactactcca gctttaactc ctaagctttt
    26161 tgtcatttga agtgcccgat gaatatgagg agttgcttcg acgtggacag taatgatatc
    26221 tgctccagct tttgcaaatg ctggaatgta gttttctgga tcaacgatca tcagatggac
    26281 gtctaacggt aatttagtaa caggacggat tgcctgaaca atatttggcc ctaatgtgat
    26341 atttgggaca aatcggccat ccattacatc tacgtggatg taatctgcgc ctcctttttc
    26401 cactaactca atgtctcttt gtaaattggc aaaatctgca ctcaaaatgg atggtgctat
    26461 cttcattctt gtttcctcct attttcgttt accttctttt tttgcataca ttggttttct
    26521 tttttcgatt tcttgtaaga attgcaaata attgtcgtaa cgtgtctgtg cgataccacc
    26581 tgtttctact ctatgtttta cttcgcagcc aggttcttta gcatgcatgc actctctaaa
    26641 tttgcaatgg acagaagctt ctacaaattc aggaaattgt ctcggcagtt ctcctgcttc
    26701 aattgtcaaa aagtcaatcg agctaaatcc tggtgtatct gctactaacc cgccatagat
    26761 aggaatcagt tccacatgac gtgttgtatg ccgtcctctt ccaagtgcat cagaaatctc
    26821 tcctgttgct aaattcagtt ccggtgatat ttgattcagt agcgtcgatt ttcctgctcc
    26881 agactgaccc ataaagaccg atagccgttc tgggaagtaa cggatcagtt ctgtcgtatc
    26941 acccgtatga ttgggtacaa tcaccggata gcctatttta gtataggttt gcttgatctc
    27001 ttcgattttt tctgggtcgt ctgacaagtc ggtctttgtc aaaaaaataa tcggttcgat
    27061 cgactcgtat tccaaggtaa ctagaaagcg gtcaagtaga ttataagaaa atgcaggctc
    27121 tacgatactt gtgacaatga ttccaagatc aacatttgtg actggtgggc gaactaattg
    27181 attttttctc ggcaagattt ccagaagata gccgtctgtt tgattttcac tctcaaagat
    27241 cacctgatct ccgacaagcg gcgtgatttt ccgattccgg aaatttcctc ttcctcttgt
    27301 ttgataggtc tctccgtttg cgtatacata gtaaaaaccg ctgattgctt ttctgatttg
    27361 tcctttgata agtgccacct ccgtttttgt ttattttacc atacgaagag gaaagtaggt
    27421 gttattccat gcaaacttct caaagtatat ggcttgaaaa ttgatcaaaa gagatagaaa
    27481 cacaaaaaag agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc
    27541 acttattttg aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct
    27601 tttgaagagt tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct
    27661 ttttcagaac tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt
    27721 cctttggtaa ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc
    27781 gtgtttgaat attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa
    27841 ctttgtgctt cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg
    27901 cttacgacaa aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga
    27961 ctgatgatcg aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg
    28021 atattgaatc cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga
    28081 ctactcaaag tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta
    28141 ggatcgactt ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct
    28201 gaactgaatt cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt
    28261 gcttcggcat cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg
    28321 ctactaatat acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct
    28381 gtcttcacta cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct
    28441 acttctaatt tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca
    28501 tcaggaacaa caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct
    28561 aaaacagcaa gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga
    28621 tcaggcagaa cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga
    28681 ggcatagatt tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt
    28741 gtttcattat ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat
    28801 actgtagaaa gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta
    28861 gccgttgccc gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt
    28921 atagatggaa gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca
    28981 aacggtacgc tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac
    29041 tgcttcgttg ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcctagc
    29101 atcgtattcg tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg
    29161 atttttacaa caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg
    29221 atttgatggt tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct
    29281 gtttcataag gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg
    29341 tattccatca ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg
    29401 tttggatgaa cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc
    29461 tggtcatttt gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa
    29521 tcgtgcgcca aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa
    29581 cggccattta attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa
    29641 gaacggtgat attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt
    29701 tatgagacaa tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt
    29761 tcgtcaagcc atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa
    29821 catctacttc gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat
    29881 ggttcactgc catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat
    29941 gatcctccgt caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa
    30001 cattggcaat cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac
    30061 cgttatattc cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg
    30121 attgaatgaa ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt
    30181 cgcctagatt cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc
    30241 cgtctgctaa tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt
    30301 gatttgtgtt acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc
    30361 attacttttt gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca
    30421 aagtcaacat tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc
    30481 tctcaaaatc agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta
    30541 agatcgtaca agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt
    30601 ctaaaatttc caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac
    30661 gaatatctgg ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc
    30721 gatcaaactg ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt
    30781 agacagcaga agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt
    30841 cgtgtacatc taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat
    30901 gcgtcgtctt acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt
    30961 gcatagacgg cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga
    31021 acaatgagct agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc
    31081 ttttttcagc tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc
    31141 ggcgtgtatc tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt
    31201 cagtttcttc gtatccgatt tgatccacca gtcgtttcgt catccataga ggcaggctga
    31261 ttttgacaga aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac
    31321 gctgataatt cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt
    31381 ttgcgatttc gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta
    31441 attggtatac agaaagataa agaagcatct tgacccagtc atccactttt tttgctttag
    31501 cgatcaatgg tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca
    31561 attctgtaaa taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca
    31621 acaaaagatt cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat
    31681 atcgtactga ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat
    31741 ctgaacttgc tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc
    31801 tggctgtaat tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt
    31861 tttgtctttc cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac
    31921 cgataataat ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat
    31981 tccgcgtact tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc
    32041 ccgtgtgatg tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc
    32101 atccaaaata ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc
    32161 aaacatcgtc cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc
    32221 gcctgcatcc atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt
    32281 catgatggca taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac
    32341 attgattgcc cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc
    32401 cgcagtgacg atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga
    32461 gattttctct ggctgcagca cacggatacc gtgcttcaga gccgcttttt ttaccggtgt
    32521 cggcgtaatt actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat
    32581 ctcgtaacct gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat
    32641 aaatactagc ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga
    32701 tctgctgtat cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga
    32761 aggcacgggc taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat
    32821 aacgcacggt cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac
    32881 aaccttctac gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa
    32941 atttctcgcc ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag
    33001 cagcgatccc tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga
    33061 ttgtctcgtc cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt
    33121 ggatcaatat aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt
    33181 gtcaattgcc acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc
    33241 caaaacatga ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg
    33301 gtttttcact ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa
    33361 cacttctttt agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg
    33421 atggctggcg gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac
    33481 atgcatttcc tgctgataaa actgctcata attttgtttt ttagccaatt ctatggcgta
    33541 atgttgagga ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg
    33601 tcctgaaact tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt
    33661 cagggaggta tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt
    33721 agcaatcatt tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat
    33781 ctgttcgtgt gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg
    33841 cggataaagt tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat
    33901 tttattgccg ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg
    33961 gcagcgcatt gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg
    34021 aagaacaaat ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag
    34081 taggacagtt tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga
    34141 aaaagtagag gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg
    34201 aagctgtgca gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg
    34261 agctcgtgct cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg
    34321 atatttactg cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt
    34381 gtaacttgat tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa
    34441 aatagcagat cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc
    34501 atcatatttt tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg
    34561 tcctttgaat cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc
    34621 cgttttattt tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct
    34681 gccagtgatt ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat
    34741 ttgttcgaca gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac
    34801 tcggtcttta taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc
    34861 ttttccttga ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc
    34921 ttcttggctc agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg
    34981 taattttgcc cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat
    35041 cgtttttact ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg
    35101 ccagcgaatc aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc
    35161 tcgctgatca aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc
    35221 atattctgct ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta
    35281 agtaattttt ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag
    35341 gtctagcaaa cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat
    35401 agccataaca aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat
    35461 cccgacttgc aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt
    35521 ctgcatagta ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct
    35581 ttttttgtct tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat
    35641 ggatttgtct tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa
    35701 gaactgtgtc aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt
    35761 gaaaaggaaa cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta
    35821 cattttttgc tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc
    35881 ttgacgttcc tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt
    35941 gactacattt cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa
    36001 agaatctaga gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat
    36061 cactagagaa tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat
    36121 cataagacta catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa
    36181 aatgttcact ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta
    36241 caacagcata gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt
    36301 tttcgattac ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta
    36361 aatctggcgg agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg
    36421 ctccttggac ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgacat
    36481 atgacaaagg agttccataa taattcccga catattctgc gtattccagc atctctccag
    36541 cttcgatcat tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct
    36601 ccccttcacg cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac
    36661 tctcaaaaat tgcttttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga
    36721 ttaataatcc acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt
    36781 ttattttgca ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg
    36841 taaaaaagct accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga
    36901 acagccaaac ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg
    36961 caagcttttg aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa
    37021 gaaaaatttt gtgaaaatag tttctcacat ttttgcataa atttggctta ataccgagaa
    37081 aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga attttgtcac
    37141 aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat tctgtttatt
    37201 tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa gtattcacta
    37261 cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct acctcttctg
    37321 tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa
    37381 taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca aagccgtttt
    37441 tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg aacgttacgt
    37501 cataatattt cattatctct acctctttcg tcattcaata ttttgtactt gttctctgat
    37561 tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg catttgattt
    37621 ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta attcccgacc
    37681 tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg ccagacgatc
    37741 cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca acaaccgttg
    37801 ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta tttgaaattc
    37861 cttttcatag atttctacaa aactatcgag tttttttagt acttctttga attcttcagt
    37921 attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg cctcaataga
    37981 atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca tgctctcttc
    38041 cggtttttct tctaccgtta cgaagttttc gtccagcacg aagcgttccg ccatccgact
    38101 aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca attgagtgat
    38161 aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc caatataagc
    38221 tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa
    38281 atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta gaaaacgctg
    38341 gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga tggctttgcc
    38401 aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt tctattgctt
    38461 tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt ttttcatggt
    38521 ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg actaggttca
    38581 gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct acgatttcat
    38641 atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata ggatcagtaa
    38701 tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag
    38761 cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct ggtattttcg
    38821 ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat catccggcat
    38881 actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat agatttcatt
    38941 gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc atgtctctgg
    39001 tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc ttttgaaaaa
    39061 agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc agtatgttct
    39121 tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta atcgtttttt
    39181 catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa
    39241 gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg gcatacgaat
    39301 ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg ctcctttgtt
    39361 aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt tattttttcc
    39421 gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc aatctggtat
    39481 ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg gcagtttagt
    39541 agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat ccaaattgtc
    39601 tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg ctgttttcgt
    39661 catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag ccatttctgg
    39721 taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt ttttcatatc
    39781 tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga taatctcaaa
    39841 gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac cgtgcgctcc
    39901 tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt gtgtggttcc
    39961 ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag attgaaataa
    40021 aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc gaaaaaattt
    40081 tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat ctcctctact
    40141 tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag cacattgctt
    40201 ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa atggaagggg
    40261 ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt aaactttttt
    40321 tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa aaggtcatga
    40381 gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct cctttaaata
    40441 aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat gaaaacgtga
    40501 ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat gttgtaaaat
    40561 aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt tatgtacata
    40621 ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac tttgcaaaca
    40681 gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct gctggagaat
    40741 atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag cgtttctttt
    40801 cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt actgatttaa
    40861 cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt ggtcatatga
    40921 aaacacatct tcattcgccg tttttttata aactacagaa taattttgta tacttcgatc
    40981 ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat aggatttttg
    41041 ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct tttttcaaaa
    41101 aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt aatttgatgc
    41161 aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa tatcgtatcc
    41221 caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat cgagaagagg
    41281 aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg gtagaagatg
    41341 ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat aaaaaggaaa
    41401 caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt tacagctcct
    41461 gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat aaaacgatga
    41521 tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa aagagccaag
    41581 gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata aaatgccaaa
    41641 aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta atttgtttcg
    41701 tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct gcgcctgcaa
    41761 aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa taaacttgtc
    41821 ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc tttgtccctc
    41881 ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa ctgctgccga
    41941 tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt ccttctaccc
    42001 aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc acaaaggagg
    42061 aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc tcgcataacc
    42121 gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga ctcccctttg
    42181 tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt tgttttcatg
    42241 gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga ttttgtcgat
    42301 tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga tagctgaatc
    42361 ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag catacacact
    42421 agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg ctgtatcgat
    42481 catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat tcattacttc
    42541 tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt tcaaaaattc
    42601 cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga caacatcatc
    42661 gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca tcaaatgatc
    42721 gactggaata tcaaagaaac cttggatttg gactgcatga aggtccaaag tcagcattct
    42781 tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg gttcccgtgc
    42841 acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt tgattgtttt
    42901 tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt tatcattgac
    42961 tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt cttcaatgtt
    43021 tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact cgacgccgac
    43081 tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa tttttaatct
    43141 tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa ctctagaatt
    43201 ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc aaaagtctta
    43261 gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg ctctcgcaat
    43321 tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa tcgaactgtt
    43381 tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc tgtggtcgcc
    43441 gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac cacatccgac
    43501 attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga ctttagttcc
    43561 tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt tttcgccaat
    43621 ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct tgacatggct
    43681 gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt ctacgatttt
    43741 tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga tttgtacacc
    43801 tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg tcgttgctga
    43861 atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc tcatgatctc
    43921 atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa aatcatccgt
    43981 ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat ctgttagata
    44041 gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca aagcttcgtt
    44101 gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg gtgttgcatc
    44161 tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc gtccatatcc
    44221 tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc cttggtgata
    44281 ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac tgataactaa
    44341 cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat gtcctgttcc
    44401 caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt taacttgttc
    44461 tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga cccgattaat
    44521 gatatgttct accataggtt taccagaaac gggatgtaaa actttatata attttgactt
    44581 catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca actgcgtcac
    44641 tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt caacaaaaca
    44701 agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc ataacgggcc
    44761 tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca cactactcat
    44821 aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa acggtaggct
    44881 tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc tcaataaacg
    44941 gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca ggacgctgat
    45001 ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    45061 tgaaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcggct
    45121 ttttcacaac ctcttattct gaaaaataat ttcctggcac aaccgtaata tttttcgttt
    45181 gagtatctac atctttgaca taaagcagtg acgtataatc atcgatggcg cgatgaccgt
    45241 tgaattttga ttcggcaaat acagtgatgc caacaagttc tgattcgaat tcttcaatca
    45301 agctcttcat tccgttgact gttccgccgc ctttcatgaa atcatccact acaaggacac
    45361 gtgaaccacg tttcaaactt ctcttagaca gttccatctt ttcaattctt tcagaagatc
    45421 cagacacata gttgacactt actgttgatc cttctgttat ttttgagtcc cggcgtacaa
    45481 taacgaatgg cgcattcaga taataggaca cagcttgggc aataggtacg ccttttgtgg
    45541 ctacagtcat tacagcatcg attttttcac caaggtattt agaagcaatg attcttccaa
    45601 cttgacgcag cagttcaggt tctcctaaca aatcagagag ataaacatat cctcctggaa
    45661 gcagacggtc ttgttctgat agacgttctg ctagagattc aatatatttt ttcgcctctt
    45721 catatgaaat ttcagggatg aacaatacac cacctgcagc acccgggatt gtttccaagg
    45781 ttccataccc tcgttcttta aatgttttct tgatgattcc caaatcttca ctgatagaag
    45841 atttagcaga ttcataacgt tcggcaaagc ttgttaagga aatcaattca tgcgggtgat
    45901 tcaataaata ctgtgtcatg tcaactagac gttcactgcg acgtactttc acctattcca
    45961 actcctttta agaaatttta tcgttttaca ttatataagt ttttcttcaa aaattcgagc
    46021 attttatgaa aaaataagaa aatagttcgg tatttattaa aataaaaaga agcctataaa
    46081 aaatttttcg tttttataag gcttcttttc tttataattt agttattttt tcttctgaag
    46141 gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa gactaatgtg
    46201 attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga cagcatccct
    46261 agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc taatcgcata
    46321 ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc aatcgggatc
    46381 atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa atgaacgggt
    46441 agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg ccgtttgaat
    46501 aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac ttgctcccat
    46561 gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga attccctccg
    46621 ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat ggcaatggag
    46681 atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc tgctaataca
    46741 acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa cgctacccct
    46801 gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat cactaaaaac
    46861 acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc tcttctcatg
    46921 aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg atctgtctgt
    46981 ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat ttcccatgga
    47041 catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca tccatacctg
    47101 ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc gctaaactga
    47161 ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata tcccacatcc
    47221 ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga cgcttgaacc
    47281 agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta ggaaaacctg
    47341 cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt ccttcttgat
    47401 ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac gatgctttca
    47461 ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca cccgcatcta
    47521 catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc aaatcagata
    47581 tttcgatata acgcacctta caacctccat tctaaaactt aattgatact ttcttttaaa
    47641 gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat ttgttcatta
    47701 gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt cgacaatgtc
    47761 tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt gtttttcttc
    47821 acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg tgaaacgccc
    47881 gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc agcatgctga
    47941 gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg atccagtgct
    48001 tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt atctggatat
    48061 ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac cggatcgagc
    48121 caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc ttcatgttcg
    48181 tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat caaggtctgt
    48241 ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga actattatac
    48301 acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc gaaatcatgc
    48361 ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac gatctctttt
    48421 gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt tttgtctgct
    48481 tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac cgtagctgtc
    48541 aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt ttacgattta
    48601 ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc atcaatttct
    48661 gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg ctcatatcta
    48721 gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca gtttttcatc
    48781 acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt cctcacaaaa
    48841 accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca aggcaaaaac
    48901 ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca tacgctcttt
    48961 gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt ttcccatttt
    49021 tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt ggatgtcttg
    49081 atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga cactcattct
    49141 tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga tttcttcccc
    49201 tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg taccgacttc
    49261 caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt ggttgatccc
    49321 tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta ctggaatatt
    49381 cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta gcaataaagc
    49441 agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg tttctacaat
    49501 gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta cgctcgacat
    49561 caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca gtcctaaatt
    49621 tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa aagtctaagt
    49681 cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct agtggttgat
    49741 tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa caagttctag
    49801 tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc ctttttcttt
    49861 tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt tcctataagc
    49921 aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag cagactggaa
    49981 ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt gaaagaaaga
    50041 aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt ccgagtagcc
    50101 cattttctcg tatacaataa acagattggc taacagatag tcagctaatt cctcagattc
    50161 tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg gctgagcggt
    50221 gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag ataatacata
    50281 tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa gccatttttg
    50341 tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc aagtaaaatg
    50401 gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag acaagtcttc
    50461 tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac ccaattcatg
    50521 aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc ctaaacaatc
    50581 tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc caactggatc
    50641 tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt gatcttggtc
    50701 gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc ttgtagctgt
    50761 gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac tgccaagctt
    50821 cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg agatgcccaa
    50881 accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat gatttcctgt
    50941 tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa cgataaacaa
    51001 aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga attttgttcg
    51061 ataaaattct tttctggata accacacagc taacagaatc aagacatata ccaatatcaa
    51121 ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta aagcaatcgt
    51181 aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga gtaataaaaa
    51241 gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca ataatacttg
    51301 aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca tcatgatccc
    51361 cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa acagtcgcgg
    51421 aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca atagaagtcc
    51481 cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa ctactgctac
    51541 atcttgccga acagacaatt gactcattcc taagatcatc aaaacgtcaa aagtccccat
    51601 tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca taggatagat
    51661 cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata ccgcaagggc
    51721 tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg gataaaactc
    51781 gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg ctaaaaaact
    51841 accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa taaaaaagac
    51901 atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata gtgcgacttt
    51961 tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac tagcccgcag
    52021 acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg ttacccacgc
    52081 agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta agactctgat
    52141 cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca gaatcatgag
    52201 caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc aactcatgcc
    52261 attagcaata tttgcgactt gatttgccac aaagactaac acggagccga aaaaaatcag
    52321 ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt tatttttcac
    52381 attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc ttatttattt
    52441 taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa agatatccta
    52501 atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat atggcacaag
    52561 ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg ttcgtttcca
    52621 gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat gcatgcaaaa
    52681 gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa ccaaaaatca
    52741 ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc atttctattg
    52801 cttcaggaaa atcattaata gatttagaaa cagcgcttga agatcctcag gcctccatcg
    52861 tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg atttgcaaaa
    52921 atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa gcaatcggca
    52981 gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct ggcagctcac
    53041 ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg aatggtttgt
    53101 ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt caaaaattcc
    53161 tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct ggcggtacaa
    53221 ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc atcaaaggaa
    53281 tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga tagcaatgtt
    53341 ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt cttttgtatt
    53401 ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac tttgatgtgt
    53461 ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa acgaacagcg
    53521 tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc agcttgaact
    53581 gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact tcttaaacga
    53641 cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct
    53701 gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg atgagaagcg
    53761 atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc accgatctca
    53821 acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag caacccggca
    53881 cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc taattttgca
    53941 acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg cagacgtccg
    54001 atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc
    54061 ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat ttcttcgata
    54121 cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg tgcgatttct
    54181 cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc gatgattaaa
    54241 tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc aatgatacga
    54301 cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga aacttgatct
    54361 gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc tgcttcttct
    54421 ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag ctcttcttca
    54481 gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat tttttctagt
    54541 tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc aattaattgc
    54601 tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt ttctaacgag
    54661 tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg agattttaat
    54721 tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg attttcttcc
    54781 ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc actatcgatg
    54841 attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga ctttgcgacc
    54901 ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat gttgaatacc
    54961 ataaaatcca cctccatact atctttttca tttttatcat ctatacagct tctatgataa
    55021 attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa catgcacaac
    55081 tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa acggctttct
    55141 acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat aggtaaacct
    55201 ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata tttttatttt
    55261 atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa aaaacggcag
    55321 gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat ttttattctt
    55381 ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact tcttcaccaa
    55441 taccataagc tgctctaact agagcagaaa cttctgccat catttcagga tgattagcca
    55501 tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa gaataccaag
    55561 caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct ccttcttgtg
    55621 agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt gctactttgt
    55681 tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt ttcaattgtt
    55741 ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt cctccaggag
    55801 tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg atgaaaatag
    55861 cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga gacatcaaac
    55921 gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct cgtggaacta
    55981 aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact aaagcatcag
    56041 cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca tcgatattca
    56101 cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg aaagcggccg
    56161 ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt ttacctgaac
    56221 tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca cctaaagcga
    56281 catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt ttttcgccca
    56341 atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca gcatctaagg
    56401 ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat cagagcaaca
    56461 tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac cgaacaaata
    56521 ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct tgcataaccg
    56581 cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa accacgccgt
    56641 ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac gtgtccggtc
    56701 cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt gcttgaatgg
    56761 ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc tccggatcaa
    56821 tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct ggaaatactt
    56881 cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc aaactttccg
    56941 ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt gctagcgaat
    57001 tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt tcttctaatg
    57061 ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt cgtaatgtca
    57121 cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca atcagatctt
    57181 ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact ttagaaatta
    57241 gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc acaaacattg
    57301 gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct gtttgataaa
    57361 aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg ataatctggg
    57421 actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat gccaataatt
    57481 ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca gttgcttctt
    57541 ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt tcacttcttg
    57601 tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca actgtttgat
    57661 aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca ttcgtattca
    57721 cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc attacttccc
    57781 gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag agggcaagga
    57841 gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc tcctgccaaa
    57901 acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg aaattatccc
    57961 ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata cttacattga
    58021 tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga tagtaaagat
    58081 cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga atgggatatt
    58141 attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt ttcccggcca
    58201 agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg tgacggctaa
    58261 ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt caaccaacat
    58321 gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact tgccaaaatt
    58381 agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga tactagctaa
    58441 agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc ctacgttaaa
    58501 ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca tacaaattct
    58561 aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc ctattctacg
    58621 tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt tattcaaagg
    58681 agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga cacagatttg
    58741 tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat ttatacgatc
    58801 attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt tcccctggat
    58861 taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca ccaatcgtga
    58921 tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt gctgccagtg
    58981 tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga ttgacagcag
    59041 taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg gcactgccag
    59101 aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt gttgattcta
    59161 ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc tcgactgatc
    59221 cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga cgatcttgcc
    59281 aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat aaaatgacag
    59341 gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct tttctagaag
    59401 tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg cttttcccat
    59461 caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat tgccgaataa
    59521 atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg attgcttcca
    59581 aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt tttttattca
    59641 ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca tcttctccag
    59701 tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt tgggtgctgt
    59761 gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct gccaactcct
    59821 cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact tgttccttgt
    59881 gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca atcagctgtt
    59941 cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt atgtttctct
    60001 aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt caaaagcttg
    60061 catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt tacttggctg
    60121 ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg tgttccagaa
    60181 gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat tttcgtaaat
    60241 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta cttcgtttga
    60301 ttttatttta atataagttg attaaaagag agcatgattt gttttatttt ctcactaatt
    60361 tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc tactacaagt
    60421 ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt ctcatgtaaa
    60481 actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg tctttcattt
    60541 ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat aaactttgcg
    60601 taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt ccaagcattt
    60661 tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg aatccaagaa
    60721 tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg ctgaagtccg
    60781 caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg ataattcctt
    60841 gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg aacaacagat
    60901 tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg tctaatcctt
    60961 taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt tgtttgatga
    61021 tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg aaattttttg
    61081 cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta ccaactacaa
    61141 ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag tcttgagctg
    61201 tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg tgtgtaggat
    61261 acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca tacatttgga
    61321 tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag taaggtgctt
    61381 gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta gaaaacagat
    61441 agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc ccaaataatt
    61501 gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg gcaattccat
    61561 ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg atcgaaccat
    61621 aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac aaatacacgg
    61681 tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca tattctgttt
    61741 tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat ttgctcagct
    61801 acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc ttcatcagat
    61861 aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc ttgcggagag
    61921 tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag ttccaactct
    61981 gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag tacttgattt
    62041 cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt atccacgcta
    62101 ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag tttagagtga
    62161 ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa
    62221 tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc acgtacttgt
    62281 cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata aactgtttcc
    62341 cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc aacatcgccg
    62401 acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga cgctagcgaa
    62461 ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt ttgttccggg
    62521 ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt atcttccttc
    62581 aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa ctgatcaaac
    62641 tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac cgcttgagaa
    62701 aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc gtttagccaa
    62761 tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag ctctgcaagt
    62821 tgcgcactta actttgtctg atccgaataa ttcaaactat ttgtttccaa catgctgctg
    62881 agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc
    62941 cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc tgccaattga
    63001 tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt ttaatgggtc
    63061 aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca attcgccgtt
    63121 ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca aaagaaacag
    63181 acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta aagacaaata
    63241 aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc gtggagatgt
    63301 cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg taaaccaagt
    63361 ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca caggtacttc
    63421 tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg tcacatttgc
    63481 gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg tcttattctt
    63541 agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt
    63601 ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc ttgccaaaca
    63661 agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata cttctaaata
    63721 ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct
    63781 taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca
    63841 tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg
    63901 atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc
    63961 tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata
    64021 ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa
    64081 aacgtatgcc aagcttctcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc
    64141 acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct
    64201 tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt attcttagca
    64261 taccgattcg cacacaaaaa agcaaacgaa ccttacgaat
//
LOCUS       DAGCXG010000007        51367 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGCXG010000007 DAGCXG010000000
VERSION     DAGCXG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.007"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(200..598)
                     /locus_tag="MB022_000440"
     CDS             complement(200..598)
                     /locus_tag="MB022_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBT4465468.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(595..1368)
                     /locus_tag="MB022_000441"
     CDS             complement(595..1368)
                     /locus_tag="MB022_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4465469.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1365..2048)
                     /locus_tag="MB022_000442"
     CDS             complement(1365..2048)
                     /locus_tag="MB022_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4465470.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILPLAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2215..3102)
                     /locus_tag="MB022_000443"
     CDS             complement(2215..3102)
                     /locus_tag="MB022_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4465471.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3099..3968)
                     /locus_tag="MB022_000444"
     CDS             complement(3099..3968)
                     /locus_tag="MB022_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4465472.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4105..5391)
                     /locus_tag="MB022_000445"
     CDS             complement(4105..5391)
                     /locus_tag="MB022_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4465473.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5664..6242
                     /locus_tag="MB022_000446"
     CDS             5664..6242
                     /locus_tag="MB022_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4465474.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6242..7171
                     /locus_tag="MB022_000447"
     CDS             6242..7171
                     /locus_tag="MB022_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4465475.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7168..8904
                     /locus_tag="MB022_000448"
     CDS             7168..8904
                     /locus_tag="MB022_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4465476.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8967..9803)
                     /gene="pheA"
                     /locus_tag="MB022_000449"
     CDS             complement(8967..9803)
                     /gene="pheA"
                     /locus_tag="MB022_000449"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBT4465477.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9800..10309)
                     /locus_tag="MB022_000450"
     CDS             complement(9800..10309)
                     /locus_tag="MB022_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBT4465478.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10302..11597)
                     /gene="aroA"
                     /locus_tag="MB022_000451"
     CDS             complement(10302..11597)
                     /gene="aroA"
                     /locus_tag="MB022_000451"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBT4465479.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFMTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11610..12701)
                     /locus_tag="MB022_000452"
     CDS             complement(11610..12701)
                     /locus_tag="MB022_000452"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBT4465480.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAI
                     EQQIVDYVANDFETGAEQADVILLAVPVRTTLAYLAVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12718..13884)
                     /gene="aroC"
                     /locus_tag="MB022_000453"
     CDS             complement(12718..13884)
                     /gene="aroC"
                     /locus_tag="MB022_000453"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBT4465481.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            complement(13886..14950)
                     /gene="aroB"
                     /locus_tag="MB022_000454"
     CDS             complement(13886..14950)
                     /gene="aroB"
                     /locus_tag="MB022_000454"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBT4465482.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKNLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14983..16005)
                     /gene="aroF"
                     /locus_tag="MB022_000455"
     CDS             complement(14983..16005)
                     /gene="aroF"
                     /locus_tag="MB022_000455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBT4465483.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16663..17637)
                     /locus_tag="MB022_000456"
     CDS             complement(16663..17637)
                     /locus_tag="MB022_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBT4465484.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17618..19501)
                     /locus_tag="MB022_000457"
     CDS             complement(17618..19501)
                     /locus_tag="MB022_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBT4465485.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGTWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19519..21743)
                     /locus_tag="MB022_000458"
                     /pseudo
     CDS             complement(19519..21743)
                     /locus_tag="MB022_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
     gene            21857..22891
                     /locus_tag="MB022_000459"
     CDS             21857..22891
                     /locus_tag="MB022_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4465486.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQATRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDVYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYRLGGWTEKQGEVLATDLLENESPLPTAILVGSDPLAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPSLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     DSFIPRTKRY"
     gene            complement(22959..23972)
                     /locus_tag="MB022_000460"
     CDS             complement(22959..23972)
                     /locus_tag="MB022_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4465487.1"
                     /translation="MKKKILKFIGWFFGIILGITFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24050..24253)
                     /locus_tag="MB022_000461"
     CDS             complement(24050..24253)
                     /locus_tag="MB022_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBT4465488.1"
                     /translation="MKNGELEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENV
                     LFEEQWEKFLLSKALQKKFGENH"
     gene            complement(24678..24806)
                     /locus_tag="MB022_000462"
     CDS             complement(24678..24806)
                     /locus_tag="MB022_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4465489.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24809..26182)
                     /gene="rlmD"
                     /locus_tag="MB022_000463"
     CDS             complement(24809..26182)
                     /gene="rlmD"
                     /locus_tag="MB022_000463"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4465490.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRTGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNSLNNKIENVHYEVGKAEKVMKKWQEKGVKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLLAEAGYVVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26293..27348)
                     /locus_tag="MB022_000464"
     CDS             complement(26293..27348)
                     /locus_tag="MB022_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4465491.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEARRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRVIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27358..28788)
                     /gene="gatB"
                     /locus_tag="MB022_000465"
     CDS             complement(27358..28788)
                     /gene="gatB"
                     /locus_tag="MB022_000465"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBT4465492.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28785..30257)
                     /gene="gatA"
                     /locus_tag="MB022_000466"
     CDS             complement(28785..30257)
                     /gene="gatA"
                     /locus_tag="MB022_000466"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBT4465493.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPNFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHTQQPAILGGKDE"
     gene            complement(30257..30562)
                     /gene="gatC"
                     /locus_tag="MB022_000467"
     CDS             complement(30257..30562)
                     /gene="gatC"
                     /locus_tag="MB022_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBT4465494.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30668..32704)
                     /gene="ligA"
                     /locus_tag="MB022_000468"
     CDS             complement(30668..32704)
                     /gene="ligA"
                     /locus_tag="MB022_000468"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBT4465495.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32721..34955)
                     /gene="pcrA"
                     /locus_tag="MB022_000469"
     CDS             complement(32721..34955)
                     /gene="pcrA"
                     /locus_tag="MB022_000469"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBT4465496.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIQYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNNWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35110..36669
                     /locus_tag="MB022_000470"
     CDS             35110..36669
                     /locus_tag="MB022_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBT4465497.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGVAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVGLGVLLGVLGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPNTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36772..38703)
                     /locus_tag="MB022_000471"
     CDS             complement(36772..38703)
                     /locus_tag="MB022_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4465498.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFIVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(38721..39635)
                     /gene="pfkB"
                     /locus_tag="MB022_000472"
     CDS             complement(38721..39635)
                     /gene="pfkB"
                     /locus_tag="MB022_000472"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4465499.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39632..40384)
                     /locus_tag="MB022_000473"
     CDS             complement(39632..40384)
                     /locus_tag="MB022_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4465500.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40541..40750)
                     /locus_tag="MB022_000474"
     CDS             complement(40541..40750)
                     /locus_tag="MB022_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465501.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40761..42089)
                     /locus_tag="MB022_000475"
     CDS             complement(40761..42089)
                     /locus_tag="MB022_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465502.1"
                     /translation="MIGEDKLLMTIQLTDLNEEERKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNKLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKGLENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHPIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42225..42380
                     /locus_tag="MB022_000476"
     CDS             42225..42380
                     /locus_tag="MB022_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465503.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42454..43041)
                     /locus_tag="MB022_000477"
     CDS             complement(42454..43041)
                     /locus_tag="MB022_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBT4465504.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDHNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43124..44374)
                     /gene="clpX"
                     /locus_tag="MB022_000478"
     CDS             complement(43124..44374)
                     /gene="clpX"
                     /locus_tag="MB022_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBT4465505.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44621..45910)
                     /locus_tag="MB022_000479"
     CDS             complement(44621..45910)
                     /locus_tag="MB022_000479"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBT4465506.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46064..46996)
                     /locus_tag="MB022_000480"
     CDS             complement(46064..46996)
                     /locus_tag="MB022_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465507.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47153..47812)
                     /locus_tag="MB022_000481"
     CDS             complement(47153..47812)
                     /locus_tag="MB022_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4465508.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47815..48840)
                     /gene="sppA"
                     /locus_tag="MB022_000482"
     CDS             complement(47815..48840)
                     /gene="sppA"
                     /locus_tag="MB022_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBT4465509.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEETVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSAPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48853..49143)
                     /locus_tag="MB022_000483"
     CDS             complement(48853..49143)
                     /locus_tag="MB022_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465510.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49552..51357
                     /gene="glmS"
                     /locus_tag="MB022_000484"
     CDS             49552..51357
                     /gene="glmS"
                     /locus_tag="MB022_000484"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBT4465511.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDKEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT        14294 a        11070 c         9193 g        16810 t
ORIGIN      
        1 aactaaaaaa ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa
       61 aagaaagtct gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac
      121 gagaaaaata caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat
      181 ttcctcttca aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa
      241 gttcttgcat tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt
      301 ctacatcaat actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa
      361 tcgcatccat tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt
      421 ctggcgaaat gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat
      481 gtaagatcgt tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca
      541 ttcctccgtc aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt
      601 gctttccccc aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt
      661 ataaatatat cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac
      721 accaaacccg tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat
      781 tgataaaata aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat
      841 tgctaaaaag atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat
      901 caagaaataa taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc
      961 gccgatccca attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa
     1021 ttcttcaacc tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc
     1081 cattaaaaag ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc
     1141 taaatgctgt cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc
     1201 aatagtcgtt atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa
     1261 actaatgata ttcattaaaa caaataggat tacgataggt gccactgcca aaatgacttc
     1321 catggtaacg atttcgattc catctatcac ttgctgccag aaactcattg gaagatcacc
     1381 cccaaaatca tcacggcaag aatcggccct aacgaagcaa taccgaccat tccaaaactg
     1441 tcattttcat ttttcttttg tttgatcata ctagtcatcc ctccggcaag gggcaagata
     1501 aatggaacag ttactggacc agtagtcact ccccccgaat caaaagcaat cggcataaat
     1561 tcggaactgg tgaaaaatga ggcgatcaaa acgcctatgt agcctatagc catcaattga
     1621 taataggata gcttgaatac gacccgaagc agcgcaaagg ctagaaatac tccagtgccg
     1681 acgctgacga tcccaatcag caacactctg ccaatctctc cgtcagatat atcatatact
     1741 tgttgtccca aaacttgcac ggaaggttca gcaatcgtga tcacgatccc gatagcaaat
     1801 cccaaactga ccaaaacggt caaacttttc tttttgatca tatatttccc tactagatct
     1861 ccgacttcca tcatagaata atctgctcca aacaaaaaga gcgtcattcc gatcatcatg
     1921 attgccgcac caaccagaaa tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac
     1981 gttaaaatga caatcaggac agtcatcggc aatatagcag caatcacttc tttaaaattt
     2041 tttagcatgt atcctcctcc tttactcttt aagtttacct taaaatgaaa gagttttcga
     2101 gcacaaactt aaaacgttat catttaattt ttctcaacat aaaataacac acgtcccaaa
     2161 agtatctttt tacttggaaa aatgactttg aaggaacgcg cttattgtgt ttttttattt
     2221 tacagagccg atcattcctt gaacgaattg cttttgcaaa acaaaaaata cgatggcagt
     2281 tggtaaagta gcgatgacta atccagccat gatcattcca tagtctggtg tataggaagc
     2341 acccatagca gataaaacaa gcggaaccgt ccgtttatca ggagattgca aagctactaa
     2401 gggccataaa taattgttcc agctactcat gaatgtgatg attgcagccg cggcataggt
     2461 atttttagcc gtcggcatat acactctaaa aaaaatactt agttctttta gaccgtctaa
     2521 tcgagcggct tctactagat cttttggaaa agatttaaca ttttgtcgaa agaagaagat
     2581 caaaaaagca gtgctaacag atggcaggat gacaaccaag aaactgttga ttcccaaaac
     2641 cgttccgttc aattgtgaaa acattcgata taaagggatc atcaatgccg caaaaggaac
     2701 catcatcgat agcagtagaa tattgaaaat taggtctttt cgtttacttt gataaatctc
     2761 aaatccatat cctgccattg acgaaatcag caatgcaaac acagtggtca ccaaagcgat
     2821 catcgctgaa ttccaaaggg aacgagtaaa attcagctca ttggaaaaca aattttgaaa
     2881 attgatgatc aagttactgc caatctttat ttttcccgat gtgatatcca cgggtgtatt
     2941 cgtcattccc aaaaccatcc agacaaaagg gaaaatggaa ataaatgata ggatgcttaa
     3001 tgctgtatac tttaatactg acccaattgt cgaacgaagt tctaatgctt gttttttgac
     3061 gttggggcgt tcgattgttt ctttttctac catatttttc atcgactttc tcctcctact
     3121 ttcgtttgga ttatcgacaa tatcacaata aaggaaacaa taacaaagga aactgctgcc
     3181 gcatatccaa aattaggcgt gaatttataa ctcaaattat agatgtattg agatagggtg
     3241 gtcgttgcat ttgctggtcc cccgccagta atattttgta cttcatcaaa aagctgcaat
     3301 gtcccgatcg tcgaagtaat ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc
     3361 actttgataa actgctgcca tttagaagca ccgtcaatag atgctgcttc ataaatctcc
     3421 gaatcaatat tttgcatgcc tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa
     3481 gtgatcgaaa gaatgatcag aactctcgcc caaattggat gagtcaacca gccaatcggt
     3541 gtgtcaatca taccaatgcc caacaacaag ttgtttacaa ggccagattg agaaaataga
     3601 cttttcatga tcagagaata actgaccatc gaagtgatac atggaagaaa gatagcggtt
     3661 cgaaacaacc ctttatattt caacttttta tcatttaaaa ggttggaaat aaaaagtgct
     3721 agaaacaaca tgatcggtac ttgtacaagt aaatacagga atgtattaaa aaaagctttt
     3781 ttaaaagttg catctgtcaa catccgctga taattaccta acccgttaaa tgtcatattg
     3841 acagggggac cactttgaaa agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg
     3901 attgatatca aaatgactgg caagattaaa aaagcatgcc cgtttttgaa taaatctttc
     3961 tgcttcattc ttttccctcc aattctatca ttgattatct acatgaaaag acaagaaaga
     4021 caatatctgt aaggcagacg tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga
     4081 agaaattcac ttttttacca ccgatcaatt agccaattgt gcttctactt gtttttgtgt
     4141 atctgctagt accttgtctg tctcttctcc attgatgatt cgatgaagag cttcagctac
     4201 gactgcttca attgcatagg tctcatgtcc gtaattcact tctggtattt cagatgtcca
     4261 tgttgaaaaa tcgtcaaaga tcttttgacc actgtaaaat tcagaagctt cttgataagc
     4321 ttcttgttca ttcgcgctaa gcatcgtaga tactagtccg atttctttcg caagagtgcc
     4381 catcagttct tcattcgaag caaacgttgc ttctaggaaa tctttcgctg aatcttctcc
     4441 agctactcct ttgatcacat accagccagc gccgccaagg ttagaagctt gcgctttttg
     4501 cagattttct ggaagtgcag gtattggtgc aatcttccat tttccagatt ggttttctgc
     4561 tccttggatc gttgaagaat accaagcgcc agttgggcta gaagctactg ctcctgattg
     4621 tatcgcagtt actccagcat tccagtcaga ggtttgttcg actagatctt ctttcaaaag
     4681 agtggcaaac aattcaagac catactttaa agatgcattg tcttggatcg tgactgtctc
     4741 cccatcttca gatgtatacc attctcctgc ctgttgcata atcatcctta ctcgaccaag
     4801 gtcagaagga ttgacttcag cgattttctt tcccgtcttt tctttgacat ctcgcgctac
     4861 ttgaatatac tcgtcccact tcagattctc catatcctct tctgtataac ctgcttctgc
     4921 catcagatcc gtacgataga agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat
     4981 tttatctcct actttgttta ccgcaaattt atacggtgca aaatgatctt cctttacaat
     5041 agcagacaaa ttagcgaatg catctggata agaagtcaaa tagccttgta tcctataatc
     5101 ttcaatcaag acgatattgg gcaatccttc tgtattcccg ctcgctaaag cggtgttcag
     5161 tttctgaacg atatcatcct gagacatcgt aacaacttct atctctgttt cttcgttctc
     5221 ataaactttt tttgcttcat tcactgcttt gatattgaat gtctcatccc acgcccagac
     5281 agtgactttt tcttctttgt ctgctgtact actagcactt ccgcatcctg ttaagaaacc
     5341 tgatgctgcc gctaaacaaa cgactccagc aattaacttt ctgatgttca tttttatccg
     5401 ctccttttcc atccattagt tgtttacgtt tgctacattc atcataccaa aatttgaaaa
     5461 cggtttcttt attattcaga tacttcaaaa atttctccta aaataataaa aaaatacaaa
     5521 tcaactatga caaaaggaat aatcttagca tctagaccaa agatcgaaat ggtgaggttt
     5581 ctttaaaata aaaatatcct tgcattttct cctagaaata agtattttta aattacaaga
     5641 ctattcttaa ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga
     5701 cgattttaga tggaatgaaa cagttgatca attggtctga gcacgggttt caattaatag
     5761 ggactttttc cagtccgctg aaagcgcttg atttttcttt ggtacatgca atcgatatcg
     5821 tggtcacaga tgtaatgatg cctgaattaa atggaatcga attatcgcgg ctattaaaac
     5881 agcaacaacc tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta
     5941 aagattcttt taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt
     6001 tttttctaaa ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc
     6061 caatcaccaa tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa
     6121 ctcgtccatt gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga
     6181 aaaacggcca ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt
     6241 gatggatagc gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg
     6301 ctatgtaatc aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca
     6361 agaagttttt ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa
     6421 tgaaatattc caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa
     6481 gaaccttgct tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag
     6541 tagttattta acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa
     6601 tttcttcacg acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat
     6661 caatcgtttc tatgcattac tcaacgcttt ggctgacgag catgttggga atgaagaact
     6721 ttctttgctc aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt
     6781 ttctattctt gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa
     6841 agacaatttc gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga
     6901 aatcaattta caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat
     6961 ctttactgaa aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa
     7021 agccaagctc tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg
     7081 ctataccgaa ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc
     7141 tcaataccgt aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt
     7201 gccaaacttc tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg
     7261 tttatttatc atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg
     7321 gtactaaaaa agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta
     7381 gccgcttttg attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat
     7441 atggaacagc ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc
     7501 catccaatga tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga
     7561 aacgacatgc tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga
     7621 gtgaatgaac gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc
     7681 gataagaaag cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt
     7741 tacggatatg ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta
     7801 gtaaatcctg agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca
     7861 aatgaagaaa atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa
     7921 acaacgattt caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca
     7981 ttactcagtc aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt
     8041 attttgtata atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat
     8101 cgacacacaa aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc
     8161 ttaaaagcta ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc
     8221 aatcaattgc taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag
     8281 aaacgttcgc ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat
     8341 acgctgacta gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc agttcaagga
     8401 atcgacagtt tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca
     8461 gtcaaggaag aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc
     8521 gaaagaatct cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat
     8581 atgatccttc aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa
     8641 ggattcatca cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat
     8701 aacggcgatg gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc
     8761 ggcatcagcc atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg
     8821 atccaatcag tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa
     8881 aatcaatcag aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta
     8941 aataaatcaa tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga
     9001 taacagccca atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct
     9061 actaatgtca ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta
     9121 gttttcaatg gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc
     9181 agcattttat gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt
     9241 actggagctg actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg
     9301 ttcaactcat tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt
     9361 tctaatgaag caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca
     9421 gcagttgttg attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt
     9481 tgtgattgtg ctagtgcttg tggatgtgaa aggatttttg taatctttgt tgctggattg
     9541 cctaaaagct gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt
     9601 ttggaaagaa gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg
     9661 acagccagat cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac
     9721 gcagtcaatt gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct
     9781 ggccctagat agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct
     9841 tttctggtgt ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa
     9901 gtcgttgtgc caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa
     9961 tagacgtttc atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa
    10021 gaacaatcgg ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac
    10081 cagtcgcgat aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc
    10141 gaaagctttt ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct
    10201 caatctttgt atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga
    10261 ttgttgtttt tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct
    10321 cctgacacaa gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca
    10381 gctccttcta gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg
    10441 attcgatgat cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg
    10501 atcagaccgt ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc
    10561 gcatcaattc tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt
    10621 ccttctgctt gagtagccac taaagctaga atgggcaatt catcaatcag acgtggaatg
    10681 atttctccac caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat
    10741 tggttctgtt cattgtgatt cgtctgggta atcgctgcgc ccatctcctc cagcacatct
    10801 aagataccgg ttcttgttgg attgaccccg acatttttta ataacagttg gctttccgga
    10861 acaagaagcc ctgctgctag aaagaaagca gccgatgaaa tatcgcctgg aacagttatc
    10921 tgttgaccag ttaatttttg tggtcctgtt accgtgattg ttttaccttc tactgagatc
    10981 cttccgccaa attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg
    11041 attacggatg tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca
    11101 ctggctacag gcatattgta tcggataggt gataaagagt tggttcctgt cacagtgatt
    11161 ggtagatact gctcattctc ttttccttgc aagtcagcgc ccatctcgcg taaaggttcc
    11221 atcactcgcc ccattggacg tttagacaac gaagcatccc cgaacagagt agtcataaaa
    11281 ggctgtccag ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtcg
    11341 attgattcgg ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcatctct
    11401 tcctcttctt cgatttcaac gcctaattgc tggaaggcat gtaaagtact cagacaatct
    11461 tctgcgcgca aaaaattttg tatagtcgtc gttccttccg caattgcccc aaacatgatg
    11521 ctgcgatgag aaattgattt gtctgctggt atcctaacag ttcctcttaa tccatgtatt
    11581 tgttgtaata attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta
    11641 gacataatga ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt
    11701 attcctaaaa tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct
    11761 tttccgatca gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat
    11821 aaatcaaaga aggcaggaat cgcgccttgc ttatggatag gcaactggtc tcgactttct
    11881 tttgcccgat tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct
    11941 atttgtgcca tttcttgctg ccaatccgcc aatcgttcca ataatatctg cttattgctc
    12001 aacaaaatat ctgtccacat aacaggatcc gaggaagcga tcctagtgat atctcgaaag
    12061 cctcctgctg ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg
    12121 actaagccag ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt
    12181 tctcttgctg taagagttac gtattttgct ttggttccac aaaatagttc ttgcagtttt
    12241 tcaacatctt ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt
    12301 ttagcatctg ctgcctgtat acctgattta tgagagccag ccattggatg tccaccgaca
    12361 aaccgaagat ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc
    12421 gtaatgatga cgtgatcaga caagttgatt ctttcaagga cagcaagata agctaaggtt
    12481 gtacggacgg gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca
    12541 tttgcaacgt aatcaacgat ctgctgttct attgcttgct cacgggtttt tgactgacta
    12601 tcccaaccaa ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca
    12661 ccaatcagtc caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta
    12721 ataatgtttc aagtagtcac ggtactcctt cactgcaact ttcaactcaa caagcgaatc
    12781 acttgggaat tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac
    12841 gacgctagct gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc
    12901 ttttgtatcg atatcaacac tttgaagtgg cttgtacaat gtagggatgg gtttcatgac
    12961 cccgcgaatg acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt
    13021 ggtacgtcgg aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc
    13081 tggtaatgaa ccagaagtga atccgacccc aaattctacg cctttaaaag cattgatact
    13141 aaccacagct tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa
    13201 tccagctgga acacctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt
    13261 ctttgtctga tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac
    13321 ttctgacgct tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc
    13381 ttggattcca ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat
    13441 tttcttggca attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc
    13501 aagcacattc cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc
    13561 tggacgcgga cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga
    13621 catgacagtt gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc
    13681 aattgttttg ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat
    13741 ccttcctcct ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc
    13801 tgacagaggt aatcctgctg gtagtccttc aataatcgct gttaattctg gtccatggga
    13861 ttctcccgca gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg
    13921 tgggaacagg aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc
    13981 ttcctcttgc tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat
    14041 catacgtaat cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag
    14101 ttggagacaa gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc
    14161 cttctccatg actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat
    14221 gaccaaaatt taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc
    14281 gtttgatttc caacgtagca gtaatgattg gaacagcatg ttccagaaga tttttttcgt
    14341 ctttcagctc gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga
    14401 tttcagcgat tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa
    14461 tcaatacacc agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga
    14521 ctgctgtctt cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa
    14581 cgaaatggat cccacgcatg aacgtcgagg ccacgaatgc ggctagatca cccaccattc
    14641 ctccaccaag tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt
    14701 ataactcagc tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga
    14761 ctggttcaaa attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa
    14821 cgtttgtatc agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc
    14881 cagcctgttc tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta
    14941 cagtaagcat tcatttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat
    15001 cttttccatt gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc
    15061 attcaacgat tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat
    15121 catgccatct gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat
    15181 tcctgtacca tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg
    15241 gacagcactt aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc
    15301 gataaaaatg acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc
    15361 attgatcgta ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc
    15421 agatagcagt ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc
    15481 agccaccatg tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc
    15541 atcagccgct tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatacgg
    15601 agaagtacgt ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat
    15661 tcgcgcacat tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac
    15721 gaaactgccg tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa
    15781 ttctcgagaa gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata
    15841 gcgtgtaaat gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg
    15901 ttctgttcct tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt
    15961 ttttatttgt tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt
    16021 cttttgttta cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt
    16081 aattcctact aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat
    16141 gacccagata atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc
    16201 ttcaacgagc cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc
    16261 tacgtggctg taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt
    16321 ttgtacccac gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca
    16381 gttttgtaag ttttctagat ttcataaaaa atcgctccaa actgatgtaa gaattgtacc
    16441 tagtatagac ataaaaaaat cgactgtcaa tcatttttat gaaacttttt taattttctg
    16501 ataattaact ttgtttttca gaaaaataac aattggtttt attgaagaaa atagtcatga
    16561 acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat aaagcagctt
    16621 tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa taataaagcc
    16681 gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg ctccccagct
    16741 attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc gtctttctgg
    16801 cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg gtaaagcgga
    16861 aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt gatctttatc
    16921 cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca tatgcatgcc
    16981 gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat aaataccttc
    17041 gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat actcaaaaaa
    17101 ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg gtaatccttt
    17161 ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt ggtaggaaat
    17221 tgtcacttgg gtttttggta ctgtattgat ttccagtgta aaaataattt caaccgtttc
    17281 agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat cgatcttttt
    17341 attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc ctaaccacat
    17401 accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc gccaaaaaac
    17461 tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt ccagtccgcc
    17521 tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg tacagtcgcc
    17581 gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat ttttcataat
    17641 aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg atcccattcc
    17701 ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc atcacttctc
    17761 tgcctgtgat tggatcagtc acttggattg cctgatcttt aaaatcccat atatagcctc
    17821 cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca cctaacgaat
    17881 tccccatgtc atgcatatac tcgcatagga tgaacggttt ttccgcttta ttttcaaggt
    17941 agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa atcgaatctt
    18001 cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg tctacttttt
    18061 tgaagtaatc gttcatttgt cgaatgtttt cttccgcata ggattcattt ccaagagacc
    18121 aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct cgatcaagaa
    18181 cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca aatgcgccca
    18241 ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg attcctgctt
    18301 ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt acgccgttga
    18361 tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg atggcgcgtc
    18421 cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt tttccattga
    18481 agtatatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag tcaatatatt
    18541 ctgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa ttgggctgaa
    18601 aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt tgctctttta
    18661 acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct acattcagct
    18721 gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca ttttgacatt
    18781 tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct cgagcaacta
    18841 agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc aagaatgcag
    18901 ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa taaggtgtta
    18961 aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga ccattcaacc
    19021 aaataaacat cgcttgttct acaccagcaa aatgaatcga gatttgtttt ccctttagac
    19081 cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta gcagctgaaa
    19141 agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga aatacatgtc
    19201 cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct aattcaatat
    19261 gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta taaaatgttt
    19321 ttggacgtac cgaaggattt ggcgaataac aaaaacgcca tgttccattt aaagactgtt
    19381 taagggagga tgtggtttct acttcttccc ttgttgcata gcaaacatga tcgctatgtg
    19441 catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata tttgctttca
    19501 tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt ggaaatcacc
    19561 ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa atatccgctt
    19621 cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata cttcatggaa
    19681 cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat cttgtttagg
    19741 agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac gataataatg
    19801 gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt tgacttgctt
    19861 tttttcttca ggagacaact ttgtcaaatc cagttcataa ccaaaaacag cgctcatagc
    19921 tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg gcacagctga
    19981 tacatgagct gtgatggacg aaatcggata agcaagactt gttccatatt ggattttcat
    20041 acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat aaataaaccc
    20101 ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg gataatcagt
    20161 tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta cttctccttg
    20221 tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca tatcccattt
    20281 gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa tcgtctgccg
    20341 aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag gcattctacc
    20401 tggctcctgc ataagaaaat cagaatgtgt tcgatacagt tcagaatcaa ccgagatcat
    20461 ttcaggttca aaccataaac caaatttcac ccctttgcta tgagcataat ctgctatttc
    20521 acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg aagaattgtc
    20581 cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc ctagctcctt
    20641 tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag tgtcttccag
    20701 ttattgacca gaatcggccg ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt
    20761 agcaactcat ggtggatctg actcattttg ttcaatccct gacttgagta agaaaggatc
    20821 atttcaggtg tttgaaaaga ctggcctgca ttcaattccc acgaaaaacg atggctgttg
    20881 atcccaccga cgactctcaa ttgattgatt tgatcttttt caagttcgaa agaatggttg
    20941 ccggaataaa caaattgaag tccgatcgct tctccagtat tttctgtcgt atgatgatga
    21001 actaaagcaa taaagttatt catatggtga ctgctcgttc ctcgacggct ttcaaatact
    21061 tttgttccag ataaaacaga ttgccgtgaa atctgtcttt cattgacatg tgctcctggc
    21121 aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt ctagctggaa actagctgct
    21181 ttttctagtt tgattgtttc tccggtttca tttcttatct ttacagatcg tgtgatcact
    21241 gctctatcag taaatatggt atagaacaaa tcgaaataaa tcgctgtttc tctatcctta
    21301 agtgaaatca tcaacgtttg tgcctctgac ttgtccatca cataagtttg aggcaagcct
    21361 tcgatctatg gcttcccatc tgtaatataa tggctgtcat atctcagatc cagtaagtct
    21421 gacccattct tgcgctgaat gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa
    21481 gagtattctt gcggtagtgt atctttcgac agagaccggt caggatttaa gggaacattt
    21541 cctgaaaatc cacgatctct tcggggataa tttttattgt ctttgtactg cttgactggt
    21601 ttgccaaaat aaatatgcgc caatacattg ctttcttcta tctttatcag ataagaaaga
    21661 aatgtatttc gtaaatggaa gactcggcta ttttcatcaa ataagatgaa atttttatct
    21721 gtttttctat gcgtaatatc catcatttca ctcccatcaa aacggaaacc gtttactttt
    21781 tttataattc ctgtatactg tttactaaac aattttgtca atagaaattt tttatttttt
    21841 tgaggagtgt gtaaaaatgg ttggaattag agatgtcgca cgaaaggcaa atgtctctcc
    21901 tgctacagtt tcccgcgtgt tgaataacga tcagacgatc catgtggctc aggctactcg
    21961 gcaacggata gtagaggcag ctgctgcgtt ggattatgac atcagtactc gtaaatatat
    22021 caaaaagaga atgccttcga ttggtgtgat ctctacgatc ggcaaagcga gcgaagaaca
    22081 ggatgtctat tacaaacatt tgcgagtagg gatggaagaa gaagctcgac gtctgcattt
    22141 agggatgaat cgaatctata atctatctga taatcctaaa caatgggaag atctagatca
    22201 gttaggtgcg gtgatcgttg ttgggactgt tacgaaagaa tcgattaccg ctttgttaaa
    22261 acaaaacatg caaatagtcg tcgtggataa ccctgacatc cagcaagagg tcgatatggt
    22321 gtatgcagat cttgaacgga tgactaaaac cgtactggaa ttgttcttag aaaatggtca
    22381 taaaagaata gcctatatcg ggggatataa agtcgatatg gatgaattcg gtgggaagca
    22441 gatttctata aacgaaaaga ggatgcgtgc ctatcagcat tttatgatcg atcatcactt
    22501 agaaaaatat atgtcttatc gtctaggtgg ctggacagaa aaacaaggtg aggtacttgc
    22561 gactgatcta ttagaaaatg aatcccctct tcctactgcc attcttgtcg ggagcgatcc
    22621 actggcaatc ggtgtctatc gggcattgaa gcaaaaaaac atccaaattg gagaagagat
    22681 tgttgtagca agcttcgata atatcgagac aattgcaaac ctctccccta gcttgaccag
    22741 tgttcaaatc aatgcaaaat caattgggca agcagctgtt cgaatggcgt tggagcgaat
    22801 tgacggtata cgtgatgaac cgatcatttt gacttatcct gcgcgattga ttgttcggga
    22861 tagttttatc ccaagaacaa aaagatattg aaaaagaggt tgtgaaaaag ccgcttagtt
    22921 tcttcatact caaaactaaa cggcaggatc acagcctctt acttatactt attcacaaaa
    22981 tccaatgtct gctggtaaca attttgcgat tcttttaacg tgtaatcaaa ttgatattca
    23041 tgagtaatct ctttttttgt atctgtataa aacaaggatt gcacgggtac ttttaattct
    23101 gtcaatcggt tttctaatgc ttgaccttgt tcttggaatg agtaagcatt tccgtcagtt
    23161 atatacgttg gcggaaattc cttagaaaca tggtcagcca cactggcttc tttcaattgc
    23221 tcactcgttt tccaatcttt tgttccaatc aacgaccacg caacggtttt gacgaaaaac
    23281 ttcataaatc gatcatccga atgctgctgt gctgttagct tcaaatcaac aggtccgcaa
    23341 taagaaatgg ttcctttcag actatctttt ggaagtagct gtttgatttt catttgttta
    23401 gcatacgcgt cattcgtttg tatagctgca tattgcaatg cgatttgtga acctgcacta
    23461 tctccaccaa aaaatagttg ttccatgtct agcaatttgt cgtctttttc ttcagctaat
    23521 aaatctttga tcagttcatt cacttgaaga acttgattag ggtattctga atcaggcgct
    23581 aattcataat tcatcgcaac tactgcgatg ttggcgtctg aaactaattt tgtcgcaaat
    23641 tctttgacac ccgatttatc tccgccgacg aatcctccac catgtaccca aaatagaaca
    23701 ggaacaggcc ccttgctgtt ttttggatag tagatgtcat aggtattctc tttatacttg
    23761 gactcatacg tcaaatctgt ttttttcgcc acgttttttt ctgcttgatc gaacgttttt
    23821 ttatcagtga tttgaacagt atttgaaaac atatggttga tcaagaatgc tccaggtctg
    23881 ggggaaagct ggaaggcaag aaagattgca aacgtgatcc caagaatgat tccgaagaac
    23941 catccgataa atttaagaat tttctttttc ataaggctcc ctcagtagtt agtattaagc
    24001 attctgatta taccatagtg ttatttccta tcaagctctc ctagatgatt taatgatttt
    24061 ccccaaactt cttctgcaat gcttttgaca aaagaaattt ttcccattgt tcttcaaaca
    24121 aaacattttc ttcttctgtt gaagcaaatc cacattgagg acttaagcac aaacgatcca
    24181 aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac ttgctggtgc tcttccagtt
    24241 cgccattttt cattgcagtc aaaatctaag acaactaatt tatcttcaga gactttctct
    24301 aaatgaacaa aactcccagc acgttcacta tcgtattcta aataataggc acggacattt
    24361 tcttgagtaa aataatgggt ctgctacgtc gtcatccccc ctgatgccgc ccaaatcgaa
    24421 taataatttc cgcgtcaaac atgtatattt agtgttgaat catttgagaa tatgaaaata
    24481 aaaatctttt tgatcaatca gttaatcaaa ttgaaataac gaaaaatctg agacagaatg
    24541 acgttcattc tgacccagat ttgattttgc acaactcatc agtccttatt ggcaaatcgc
    24601 caaaataatg tagactaact aaaaagacta agaaacaata acaaaagtta acgcttctta
    24661 gtctttcttt agcacgtcta tttattaaag attttagggc cttttctcat gtttttacga
    24721 tcattcttag gtgtttccat ttgtttggat cggcctccac cttgtttctt tttgatcaat
    24781 tctttcattt tttctttttg gtccattttt attctccttt aacgaatgaa gcgactaatt
    24841 cgatatgatg ggtttgcggg aaaagatcta caggctgaac tttcttcaca acataacctg
    24901 cttctgccaa taacttagca tctcttgcaa aagttgctgg attacaagag atatagacga
    24961 ttcgttctgg tgacatttcg attgctgatt caataaaatg atgatctaat cctttacgtg
    25021 gagggtctac tacgatcaca gatggtttga cccctttttc ttgccatttt ttcatgactt
    25081 tttctgcttt tccgacttca taatgaacat tttcgatttt attatttaag gaattgaact
    25141 gtgcatcttc aattgcttct ggaataactt ccatcccata taccttatct actctgtcag
    25201 ctagagacaa tccgattgta cctattcctg aataagcatc gatcacaatg tcttttttat
    25261 ccaactcggc aaattcgatg gctgtttggt acaaatactc tgcttgttga gtatttactt
    25321 gataaaatga cttcgctgag attcgatagg tttttcctaa tagctgatct tcgatatagt
    25381 ctctgccgta taaaactttt tctttgtctc ctaaaatcac atttgttttt tcttcattga
    25441 tattttggat gaccgaaacc acttctggca attcttcatg aatggcttca gcaatttcct
    25501 tggatttaaa gaatttttct ttacgagtaa ctaaaacgac catcatttca tgggaataat
    25561 gaccacgacg gataatgatg tgacgaagct gtccttcatt tttttcttca ttatatcctc
    25621 gtacttggaa ccgttgcaag atatctctga ctactttgat tgcttgatcg atgacaggat
    25681 cttggatata aaaatcttcg atagggacta gatcatggct attctttttg taaaaaccgg
    25741 tggctaactg tccatcgata cggcgaacag ggatctgtgc tttattacgg tagccaaatg
    25801 gttctgccat tccgaccgtt ggaagtacag gaacttctgg catcttagca atcttcgcca
    25861 ttacatgctc tacttgtttt tgtttgaaga caagctgttg ttcatacttc aaatgggcta
    25921 aaggtgcgat acctgtgcga agtaatgcat tgttttctac aggctgacga tcaggtgatt
    25981 gtgtaataaa tgtttcgact tttccatatc caaacttatt gcctgctttc actactagta
    26041 tttctacttc ttctccaggg agtgcatttt cgataaataa tggataacca tcgatttttg
    26101 ctacccccat tccttcataa ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct
    26161 ttactatttg ctgttctgtc atattttgct cctttaagcc tatttttcaa tcctactgaa
    26221 ctctactata aatcagctaa ttacacaact aaaaccggca ctgaccttac tacgttcagc
    26281 accggctttt gattaattct tttgattagg tgtttcgtct gctatttttc cgtcaccgtc
    26341 gatatcttca tccgttaaac gttcgacttc ttttacgaat tctttgctga caacctctaa
    26401 ttcttcttca tctgtgcctg tgattgcttc atcaggaatc tgatcggtat tggcaaagaa
    26461 ctcaatatgc tgatgaagat tttggaaatg catcggtgct tccccaccat attcaccatc
    26521 taagttgatc attactggtc gattgatatc atcttctgtt tcgacgaaca aactgcttgt
    26581 tttggtatag attactcttg gatcatcgac atgacgaccg ccatttagca tgagtgccgc
    26641 aatatgcaag atctcaaaga tatttgctgt cttcacaatg attaaagaaa acttcccatc
    26701 atccaatttg gcatctggcg cgatctgttc gaatcctcca acagagttcg tcagccctag
    26761 gaaaaacatc gaagcatttc cgatatattc cccttcatca tacttcaagc gcattttgat
    26821 gggctttact cgagggagca tctctgctcc ttttgcaaga taagcaagat aaccaaaaat
    26881 actctttagt tctgatggaa cttcataagt caattctgtc aaatagccac cggcagcgat
    26941 attgatgaag taactgttat cagaaaggcc gatgtccatt tttacggttt gatgcttttt
    27001 gatcacttct gctgctgctt taatattatc tctaggaatt tttaatgctc gagcatagtc
    27061 gttcgttgta ccagctggga tgatggccat tttaggacgg tgttcaagcg gagcgattcc
    27121 attaaccact tcgttgattg tgccatcgcc accagctgcc actactagat caaaacctag
    27181 ttctgcgact ctacgtgctt cattcttggc tgaatcaggt tctggtgtag ttgcataggc
    27241 gctggcttca aatcctgctt cttctaatgc ctgtaggata tccgccaaat tttttttcaa
    27301 taattctttt cccgaggtgg ggttataaat tacgcgtgct tttttcattt tcagcctcta
    27361 tcgtttcgct aattcatctt taagtagctt attcacgaca ccaggatttg cttgtccttt
    27421 agttgctttc atgatttgac cgactaggaa gccaaccgca cgatcttttc catttttgaa
    27481 atcatcgaca gattgttggt tgttatccaa aatttcatta atgattggca gtagtttgtt
    27541 cggatcagat aattgcaccc aaccattttt ttcaactact tcttgggctt ctccgccatt
    27601 tgtgatcaat tcacggaaga cttttttggc gatttttgag ctgatcgttc catcggcgat
    27661 caaacgaatc attcctgcta agttctcagg tgtcaatttt gtctcagcta attccagttt
    27721 ctcactgttc aagtaagcag aaacttcacc catcaaccag ttagatgctt gcttggcatc
    27781 tgcaccattt tccaaaacag cttcaaagaa atcagacatt tcttttgtaa gtgtcaatac
    27841 cattgcgtca tactctggaa gaccaaactc acggatatag cgttcgcgac gagaagctgg
    27901 catctcaggc aaactagctt ttactttttg aatccattcg tcatcgatca cgaatttcgg
    27961 gatatcaggc tctgggaagt aacgataatc actagaccct tctttgacac gcatcaaaac
    28021 tgttttattc gttgtttcgt caaaacggcg tgtttcttgt tggatttctc ctcctgaaag
    28081 aagaactttt gcttgacgtt tttcttcaaa agctaaacct tttttcacga aagataaaga
    28141 gttcaagttt ttcaattctg ctttcgtacc gaactcttct tgtccataag ggcgaagaga
    28201 aatattggca tcacagcgca tagagccttc ttccattttc acatcgctga cttctgtaaa
    28261 ttggataatt gatcgcaatg cttctagata agcgtatgct tcttctggtg aacgcatatc
    28321 agcttcagaa acgatttcga tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata
    28381 accgccaata ccatgcatgt tttttccagc atcttcttct aaatggacac gttcgatccg
    28441 gatttttttc tttttacctt ctacttcgat ttcgatccat ccgtcatgac cgattggttg
    28501 atcaaattgg gaaatttgat atgcttttgg attatctggg taaaagtaat ttttccggtc
    28561 aaaatgagta tctttagaga tttcacagtt taatgccaaa gctgctttca tcccgaattc
    28621 cagcgcagct ttattcatta ctggcagcac gcctggataa ccccagtcga tcacgtttgt
    28681 attgctgttt ggttctgcac caaaatgagc tggcgctgga gagaaaattt ttgagttggt
    28741 ttttaactcg acgtggactt ctagtccaat gactgtttca aaattcattc gtctttccct
    28801 cctaaaatag ctggttgttg tgtgtggaat tctgttgctt gttcaaacgc atatgccgct
    28861 tgatacatcg ttttttcatc aaaataatta ccgatgatct gcaatccaac tggtaatcct
    28921 tctgacaatc ctgccggtac agacatacct ggaagtccag ccaagttgac agggatcgtt
    28981 aagatgtcac tcatgtacat tgtgattgga tcgttaatgt tttcaccgat tccgaatgcc
    29041 actgttggtg aagatggccc gataatcaag tcataatctg caaagacttt ttcaaaatct
    29101 cgtttgatca atgtacggac ttgtcctgct tttttgaagt aagcgtcata ataacccgca
    29161 cttaaggaga atgtacctaa catgatacgg cgttttactt cttcaccgaa tccttctgaa
    29221 cgtgagttga catatacatc atctaatgat ttcacatttt cagagcgata accgtagcgg
    29281 ataccatcga atcgttgcaa gttggaactt gcttctgatg aggcaatgat ataataaaca
    29341 gccacaccgt attttgaatg aggcaagctg acttcttcca caacagcacc taatgaacgg
    29401 aatgtttctg ctgctttcaa tacagcttct tttacgccag gggctacacc ttcgcctaag
    29461 tattctttag gtaaagcaat cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa
    29521 ttaggaactg aaacgcctga agaagtaccg tctttttcgt cataaccgct gatagctgtc
    29581 aatgctaatg cattatcttt caccgtacgg gtcattggtc caatttggtc aagagatgaa
    29641 gcaaaggcaa tcaaaccaaa acgtgagaca cggccataag taggtttcat ccctacgatg
    29701 ccgttaaaag ctgctggttg gcggatactt cccccagtat cactacctaa agaaactgga
    29761 atttgtccag ctgcaactgc tgctgcagaa ccaccagaag agccaccagg tacttttgtt
    29821 gggtcccaag cattttttgt tttcttgaaa taagaagttt ctgtacttcc acccatagca
    29881 aattcatcca tgtttagctt accaacaggg atcatatctg cttgatagat tttatccatg
    29941 accgttgcat cataaattgg ttcaaaattg taaaggatct tagatgcagc ggttgtcaag
    30001 atatcttttg ttacgatatt atccttgata ccaattggaa taccagcaag gacgttgctt
    30061 tcatcaatcc ctttttcatc taaagctttt gcctgttcca atgctttttc ttcgttcaat
    30121 gtaatgaatg cttcgacctc aggctctgtt tctttgattc gttcaaatgt tgcttttgtc
    30181 aaatcttgta cagtgatttc tttcgataca agcatatgat gcagttcttc tagagaacga
    30241 ttgtgtaatt ctgtcattat gcgccagcct ccccattatc gatgattgcg ggtactttga
    30301 taaagccgtc ttctgtttcc ggcacatttt tcaataattc atcgcggttc catccagctt
    30361 gcgcccgatc ttgtcgcatc acgtttactg tttcaaccac attcgacgta aatggaacac
    30421 cttctgtatc tacttcttcc agcatttcaa ccatatcgat gattttacct aattggtcgg
    30481 taaaacttgc aagttcttca tctgcgaacg caagttttgc taatttggca acgtgtttca
    30541 cttgttcttc actaatcgcc atactacccc ttctttctaa acgagttaaa aactttgctc
    30601 tctattttaa cacaagagtg ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac
    30661 tcatttttca ttcttctact gagcgacttt tagccaatgc atctgccatt tgatcttctg
    30721 tccaaacttc gattcctaat tcttgtgctt tggtcagctt gctgccagca tcttctcctg
    30781 ctaccacgat atccgttttt ttcgatacgc taccagtcac tttcccgcca agattttcga
    30841 tcgtctcttt tgcttcttca cgagtaaatc gtgttagttt accagtaagg actactgttt
    30901 tttctttaaa aggagactcg acttcttgca attgggcatt tcgaagccct ttgtattcga
    30961 aattgacgcc tgcttgtttt aattcattca tcaattcatg gacttcttcg ttactgaaat
    31021 aagtgactac actgtcggca atgatatcac cgatcgtatc aagcgcaata atcgactcat
    31081 agtcactttt actcaatgtt tctagatcac cgaaatgttc tgctaaaatt tttgctgctt
    31141 tcgctccgac atgtcggatt cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc
    31201 tgttgtcgat tgctgtcaaa atattatttg ctgatttttc ttttattttg tccaacgtca
    31261 ataactcttc ttctgtcaat ttgtaaagat cagcaacatc tgctactagt tttttatcat
    31321 acatctgttc tagtacgcgc ggccccaacc catcgatatt catcgcattt cttgaaacaa
    31381 agtggttcaa accttctttc atttgagcag ggcatttagg attgatgcac cgtaaggcta
    31441 cttcttcatc caaatggaca agttcacttc cacaaactgg acaatgtgta ggcaattggt
    31501 attcttcgct gtctttaggt cgtttatcta acacgacttg cgaaacttct gggatgatgt
    31561 ctcccgcttt ataaatcaga actgtatctt tcaagcggat atccttcaac ttaatataat
    31621 caccgttatg caggctggca cggcttactg tcgttcctgc tactcggact ggtgtcatga
    31681 tagctgttgg cgttactacc cctgttcgac cgactgtcca ttcgatgttt tcgattagcg
    31741 tttcaacttc ttctggcgga aacttgtaag ctgctgccca tctcggtgct ttgacagtaa
    31801 aaccaagttg atcttgtagc gagaattcat tcactttgat gacgattcca tcgatttcat
    31861 agggcaaatc cacacgtttg tcatgatatt cttcaatgta tgaccacact tcatcaatcg
    31921 aatgacatag acgtttttcc cgattcgtgt ggaatccaat tttttctagc tcttctagcg
    31981 catcatactg ggtttttgct tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt
    32041 ccagatttct tttcgctgtg attttagaat ctaactgacg caaagaacca gctgcggcgt
    32101 tccgaggatt cgcaaagata tcttttccct ctgcttctct gtcttggttc aattggacaa
    32161 atgaccgttt tggcatgaaa cattcgcctc ttacttcgat gttcattggt tcttttaatt
    32221 tgattgggac cgaccggaca gtttttaggt tttcagtgat gttttctcct acagttccat
    32281 ccccgcgagt cgcacctctc acgaaatttc catcttcata acgcaaagaa acggaaagtc
    32341 catctatttt tagttcacag caatagtcaa ctacacgtcc cactgctttt tgtacacgtt
    32401 gatcaaaagc tatcagctct tctttgctga aaacgtcgtt caaactgtaa agcggaatat
    32461 catgagtgac tttttcaaaa ccttctaata cttttccgcc tactcgttga gtgggagaat
    32521 cagacgtgat caaatcagga tattgttcct cgattgctac taattctgcg tattctttat
    32581 cataaacata gtcttccact gttggtttat cttctacgta gtattcgcga gaccattggt
    32641 tcagtcttgt acgaagttct tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt
    32701 ccatcattta ccctccacta ctaagctttt tcaattggcg caaaggcagc caaaagacgt
    32761 tttactcctt gacttgggaa agccacatct agttctagat ccttcgacgt cccgcttact
    32821 cggacgactg ttcctagacc ccattttttg tgtttgactt tatctccagg tttccagttg
    32881 ttattttccc ctccagtagc acttttagag ggaacaaccg ctgtctctgg ctgtttatag
    32941 ctatatgctg tttttttagc gaatacaggt gtttttttag gcgtaggacg catgccttcg
    33001 atttccagta actcctgatc gatttcttct acaaaacgag aaggtcggtt atattgcgtc
    33061 cgtccatata atgttctgga gaaagcattt gttaaataca acgcttcttc tgctcgcgtg
    33121 atccccacat atgcgagacg gcgttcttct tccagttcgc tttcttccat caatgctcga
    33181 gataaaggaa aaaccccctc ttctagacca atcaggaata caactgggaa ttctaatcct
    33241 ttggctgcat gcaaagtcat caatgtcact tgggaagcat cttcttctaa attatcaatg
    33301 tcggaaacaa gtgccagatc attcaagaag accgttagtt tttcttctgg tgcatcagcg
    33361 tcttcttcat tttgctgttc gaattgcttg tcgaattctt gtgtgaccgt taaaaattcc
    33421 tccaagtttt cgatgcgtgc ttgtgcttct aatgtatttt ggatttttaa gtcttctaga
    33481 tatccgctac gatccaaaac ctctttcgtc aattctgtca ctgtcaagta ttggatcatc
    33541 tgagtgactt cttggatcat ctctccaaat gcacctaatt gctgtcctgc ttttccaccg
    33601 atatttgcca gatccacatt ttgcgctgct tcaagcagtg gccattcatg aagagaagca
    33661 aaactgcgta gtttttcgat agatcctggt ccgatgcctc gtttgggcga attaacgata
    33721 cgctcaaaac tgattgaatc ttgaggattt gccagcacat tcaaatacgc taaaatatct
    33781 ttgatttctt tacgatcgta gaacttgtgc cctccaacca ttttataagg aatatttgct
    33841 ttcaacagag tttcttccat tacacgagat tgtgcgttcg tccggtacaa gatggcaaag
    33901 tcgccgtaat tccgatgatt gcttcgaatc tcttcttgca tccggtctac gataaaacgg
    33961 gtttcgtctc gttcattatc tgcacggtag taagtgatct tgtttccttc tttgttttct
    34021 gtccacaagt tttttggttt tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta
    34081 aggatgtttt tagtggagcg atagttttgt tctagcaaaa ttactgcagc atctggataa
    34141 tctttttcaa aatccaagat gttctgcata tctgcaccgc gccagccgta aatactttga
    34201 tctgcatctc ccacgacgca caggttccta aatctacctg ccaataaatt gactagcgta
    34261 tactgtgcgt gatttgtatc ttggtactca tctacatgaa tataatggaa tttgttttga
    34321 taatatgtga gtgaatcagg atgttcttca aataaccgaa tcgtattcat gatcaggtcg
    34381 tcaaagtcca tacattgatt atattgaagc tctttttggt aagcagcata acattttgcc
    34441 gcaatctctt cgtaataact gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg
    34501 ttttttgctt gagagattgt tcctaatata cttcgaggat catatttttt cggatcaatg
    34561 ttcagttctt ttagtatccg tttcatcaag gtcagctgtt cagaagaatc gatgattgta
    34621 aagttccggt tatacccgat aaaatcaaca tctcttctta aaatacgcac acacatcgag
    34681 tgaaaagtcg atacccagac atcttcgcct ccagatgcga gaatcgcatt gacacgttct
    34741 ttcatttctt ttgcagcctt attggtaaaa gtaatcgcta ggatgttcca aggattgact
    34801 tctttttcct caatcaaata ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct
    34861 gcacctgcca ttagcagtaa tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg
    34921 ttcatcccgt ttaataattc agctttggat gacattaaca acatcctttc ttttatcaat
    34981 cttgttaatt atagcatttt cttggctttc attaaactag ctagttcaaa atcatttgtt
    35041 tctttttatg gatttttcct tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga
    35101 ggcgtttgaa tggaaaaaaa aggaaaaatc aagaaattag ataagactca gatatatttt
    35161 attctaaaag gaatatttgt tggactgctg acaggaatcg tcgttagcct gtttcgatta
    35221 tctatcgaaa agctaagcga tttagttcga agtatctatg aaatgagtcg ggaacagccg
    35281 atttacttaa ttggcgtagc tatttgctgc atccttgcag catttttcgt gggttatctg
    35341 gtcaaaaatg aaccggatat caagggaagc gggattccac aagtagaggg acagcttcgc
    35401 ggggaattat caatgaactg gttctctgtt ttatggaaaa agttcattgg cggtgtactt
    35461 tcagttggtg cgggcctttt tctaggtaga gaaggacctt ctatacagct tggcgcatcc
    35521 attggacaag gagctggaca attgtttcgg agtccatctt cagaagaaaa gattttaatt
    35581 tctagcggtg caagtagcgg acttgcagca gcattcaatg ctcctatcgc aggattactc
    35641 tttgttttag aagaaatcca tcactccttt tctccacttg tttggctgac atcatttgct
    35701 tcagcaatca ctgctaactt cgtctctctt tactttttcg gcttgagacc tgttttatat
    35761 ctaggtgatc ttccttcttt gccgttgaaa tattatggtt ctttagtggg attaggagta
    35821 cttctaggcg tcttaggttt tatttatcag aaggtcttgc tggctttgcc aaaattttac
    35881 aaccgattgc ctttaccagc ttattttcac gggttcgttc ctttcttgct gattttgcca
    35941 attggttatt tatggcctaa tactctaggc ggaggcaatc aaattgtttt agcttttgga
    36001 catgcttcct tgccaatcat tatgattatc ggtttgtttg ttcttcgatt cgttttttcc
    36061 atgatttctt acggtggtgg tcttccaggg gggattttcc ttccgatatt gacattgggg
    36121 gctttaatcg gtagctttta tggaaacatt atggttgatt tcgggatgga tccaatctac
    36181 atcaaggatt ttgtcgttat tgccatggct ggttatttca cagcgattgg aaaagcaccg
    36241 ctgacagcaa ttattttagt gacagaaatg gttggctctc ttaatcatct gatgccttta
    36301 ggattggctg ctcttgttgc ttatatcgtc aatgaccttc tcggcgggaa tccaatttac
    36361 gaatcattat tggaacgtct acttggaaat actcagaccg cctcactaaa aggtgcaagg
    36421 acaacttttg attttccagt aacggcagag agttctttag acggattaat ggtacgagac
    36481 ttcaattggc ctaaaaatat gctgctaatc tctatacgca gaggaaatca ggaaatcttg
    36541 actcacggtg atacgatcat gaatgtagga gatattttgg ttatcttgac tgatgaagca
    36601 aacttgccaa gaatcaagca ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa
    36661 tcattttaac gaacatggaa aaactatttt acaacaacta ttcatctaca ctacttgatt
    36721 ctataaaaaa aaagaagagg ctggggtttc ccagtctctt cttttttatt tctatgcttc
    36781 tgctgctgca ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat
    36841 agcaataaac accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc
    36901 gtgaggagcc agtaatttga tcccgaaagc tccgactaat ccgccagtta aagcagaacc
    36961 aataataaaa cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc
    37021 cgtgacaaac gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct
    37081 gaatttattc ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc
    37141 agccataaca gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc
    37201 tgtaccaaac acataagcag ctttgttgat tggaccgcct aaatctgccg ccatcattcc
    37261 agctaataat gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt
    37321 caatgcatca ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat
    37381 cagtaagcca aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa
    37441 gttcttgaat aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag
    37501 tgcacctaag aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat
    37561 caaaccaggg cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa
    37621 accaaatgca gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag
    37681 atttgataat tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat
    37741 agcgattcct ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt
    37801 gtaaattctt tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc
    37861 atcaccatgg aatactggtg cagtaccact aagggctaga ttgatcagct cttctggttt
    37921 acggatacca tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc
    37981 tacttttttg tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa
    38041 tcggtttttg attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt
    38101 cgcttttttc ttcagagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc
    38161 tgtaacggca acaatgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc
    38221 ttgttctgct ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac
    38281 ttcttctggt gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg
    38341 agatagtgct gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat
    38401 gaaaaacaga taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt
    38461 tgcgaataaa actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc
    38521 atcaccaaga cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc
    38581 gatatcggaa atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt
    38641 tttatcagtc gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt
    38701 catgattatc ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca
    38761 tcaatcaatt cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact
    38821 ccccatttaa aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccggca
    38881 atcattgaat cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta
    38941 taagttcctt gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc
    39001 tgtgcccctt cagctagcaa ttttttacca tacggaatga tttcttctac agattggaag
    39061 gttgtattgt aaagctcagc taattcgtga ttgtttggtt tgatcaacaa tggtttctta
    39121 acaagtgcat tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct
    39181 cgaatgatat caatcagttc ttgataaaat ccttttcgca ggctagccgg cgcgcttcct
    39241 gacaatacga cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt
    39301 tcttcatctg aaatagatgg gcccaaaccg ttaatttcag tttcagtatc tgattttagt
    39361 ttgatattga ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt
    39421 tcaagccact tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat
    39481 tgagaatcga tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg
    39541 aagtcttcct tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa
    39601 tcaattgatg gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct
    39661 tttcacggaa ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt
    39721 ccaaaggtgc aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa
    39781 atgtctcttc ggaatttttc atggctaaac gtttaacagc tgcctcttct ggatccggag
    39841 tcgtgaatcc ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa
    39901 aattttttag ttgttctatc gcattagatc ctaaaacagc attagttgcc agcttgatcg
    39961 ttccacctaa aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg
    40021 aatttgtcac tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag
    40081 agccagcgtc taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga
    40141 tttgctgttt ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca
    40201 ataattgctc tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct
    40261 cttgtaaatc acgtcttatc gttgattcag aagcattcaa aaggtttaca agatcttgtg
    40321 atttcacgat tttctgctgt tcaagcaagt ctaggatttt ttgtctgcgt tcctctgtaa
    40381 gcattttcac gatcacctca atacaaataa tagcatattt tttttctact cgcaatataa
    40441 atcagtcaaa aacgttcata aaaattataa atcagtcaaa aaaataaaaa accagtcaaa
    40501 aagactggtt ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc
    40561 ttcttgtttt tttcatataa acagaagcag gttttctttc cttatgaaga agataataat
    40621 aaagtcccat cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag
    40681 gaacaaaaaa gagacagacg gataataatg atgccggaac cattattcga taatagagtt
    40741 gtttatccat tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg
    40801 taatctaaaa attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt
    40861 cgccgctttt tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt
    40921 aacggtaatt ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct
    40981 tgatattttt catctgttac ctctacttct agatattccc taatataata acaataagct
    41041 tcgattccat tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgcgag
    41101 cgattctcgt caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt
    41161 tctttataat ctccttgttg aatatcataa ctttttgtta caaaaaatga agcattcttt
    41221 ttaggaacag gttgcatttg tgcattctga tactggacag attggatcgg atggtcagtg
    41281 taaatgtatt caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga
    41341 tcaaacccaa gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag
    41401 ttaaaggggt ctcgataaag ctggatacat atgacttcgt tttctaatcc cttcactttt
    41461 tctacttgtt ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta
    41521 taatacattt cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga
    41581 aattggttgc aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg
    41641 atttcatcct ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata
    41701 aactcgctat aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg
    41761 aagtctcgaa tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc
    41821 aacaatttat tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac
    41881 caactttcgt ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac
    41941 ggatccgctt gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa
    42001 atagcccaat cgcttatttc ctgttttctt tcttcttcat ttaagtctgt gagctggata
    42061 gtcatcaata atttatcttc gccaatcatt ttctcacctc tttcaaatct ttttataatt
    42121 taattataac caatgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg
    42181 attacatgat agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg
    42241 aacgtacaga taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag
    42301 ttgcaccaaa agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa
    42361 aagacaagaa acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga
    42421 aagagtttga ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc
    42481 tgcccacgct tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt
    42541 gtgatcaaaa tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg
    42601 gatcttatct gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg
    42661 atacatctgg atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc
    42721 acgtagctgg tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc
    42781 agttttggat acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc
    42841 gatcaaataa aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt
    42901 ctttcgatca atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag
    42961 agctatttct ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat
    43021 ttcagcttga tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg
    43081 ctgtcgcaaa agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct
    43141 ctgaatcttt atagattact gttggctcac cagacaattc agctgcttct tttgtgataa
    43201 tcactttttc gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt
    43261 caatgatgga acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg
    43321 ctctcaacgc ttcaggttca aattcaagtt cggtattatc tagagaaaga agtttttgat
    43381 attgtttgac tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca
    43441 atttctctaa agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt
    43501 ttagtaaatc ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt
    43561 tttgtgtacc aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc
    43621 catcaaatgc accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt
    43681 gatgtggatg tttacgtcct ccctgaggtg gcacgcttgc aacagttcct tctagaattt
    43741 tcagcaaggc ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc
    43801 ttttacgcgc aattttatcg atctcatcaa tatagatgat ccctttttct gcacgttcca
    43861 cattgtaatc agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat
    43921 aaccagcttc tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg
    43981 ctaatgtttg tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc
    44041 ttttttgcag ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt
    44101 gattgtatac ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat
    44161 tcaatacttc aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt
    44221 cataaaattc ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga
    44281 cccctggacc agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc
    44341 gtacggtctc attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa
    44401 gtatgcaaga aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa
    44461 aagaaaaagt gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac
    44521 aaaaaataag gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt
    44581 ctagaagtag cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg
    44641 ctgtacctgt gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt
    44701 ctgtcaacac gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga
    44761 tttctgcatt gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga
    44821 tcacaagatt tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt
    44881 cttctgtaga accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt
    44941 tgttcaagaa ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt
    45001 caacgatttc tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg
    45061 ctgctttttc ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg
    45121 ttgatacttc gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg
    45181 cttttacttc atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt
    45241 gataatcttc tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta
    45301 attgatcttc aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc
    45361 cttttccgcc atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat
    45421 cgccattttc agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac
    45481 gtttgatacg tgcatccacg tctgcatctg ttacttcacg gtcttgtttt tcaacaacta
    45541 gatctttata ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc
    45601 agtcttgacc tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc
    45661 cagcttcttt gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat
    45721 ataaagcttc ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga
    45781 atccaggtac gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga
    45841 tttcgtcttg cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg
    45901 ttgcagtcat ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca
    45961 tgcatacctt taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa
    46021 tccttgtttt tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca
    46081 tttgctgcaa aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt
    46141 catacatcgt ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt
    46201 cgatatatgt atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttctttta
    46261 acacggaact aagcagcgca tcttcattcc cgtaacgatc tgaaaataca cataattgct
    46321 ggtaacttgc ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt
    46381 cttcaatcgt caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta
    46441 attttgccct tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga
    46501 taaactcatg ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt
    46561 ccaacgaagg aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt
    46621 gtccggataa ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat
    46681 aaaaatctgt acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca
    46741 agtcaaaata aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg
    46801 cttgcttcaa tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat
    46861 tagcctcaaa gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat
    46921 attgctggtt tgctagtgct tgttgtgcta ctcgcattaa attctcatat ttttctggaa
    46981 aatcaatcgg cttcacgcag tgtcctcctc ttgctcatgt tcagctcatg ttcaatagta
    47041 gcaaaacaaa aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt
    47101 tagatctgac ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt
    47161 tgacttcctg gttgaatgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa
    47221 aatcgcccag atgttgcttt ttctgattaa agattgctgg aagataccct aacatcagca
    47281 aaggaaattt tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa
    47341 gttcttcttc atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttggc
    47401 catgattgaa tttcgtcaat aaaataaaat acgctagata aataagtaaa gctgtgaatc
    47461 cgtagagact cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa
    47521 gtgttaaacg cgtaatagct ccgatacaca aaagatcgac caagaaagca aacattcgga
    47581 taaaaaaacc tgcatagaaa tacttaggaa aatcatttgc tttcggcggt tcatttcttg
    47641 tgtccgtata ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct
    47701 ctctttcaaa agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct
    47761 gctgttgctt atctttttct tgttccttat tttcatttga aagctctgac atgcttactc
    47821 acctccgtaa taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga
    47881 taagatctga ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa
    47941 ccatgtacta gcaaacccag atggcgcgga agaatattct actagttccg catcttcaag
    48001 ctgttgttcc tttcgcatag cttttaaagc atcttcagga aatcccaatt catcgactaa
    48061 acctacttct tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc
    48121 ttcttcagat ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc
    48181 gatatatgct tgaagcaccg tttcttcttc ttttgtctct ggtcgcgttc ctgaacccat
    48241 atctttcaat gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc
    48301 tgaataattc aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc
    48361 aaagatttta tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat
    48421 gctgacatac attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc
    48481 cgcactttcg tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt
    48541 tggatcttct tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga
    48601 agcaaataaa cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat
    48661 tttttgcgta ctgtctccct cttccaacac atttggagct agttcttcat ccccatataa
    48721 ccaactgttc aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac
    48781 taacgaaacc acaaataatc ctgcagcaat cagaactgct gcccaacgtc ttttattcat
    48841 tctttctcct ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt
    48901 gtcgaaccgc tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgtcaattc
    48961 atcttctacc gtaaactcac gagcttgagc aacttccaag gcatcaggtg cgatttcttg
    49021 gatgctatcc cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc
    49081 ttcgaatttg gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc
    49141 catttttact cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga
    49201 tagataaaaa cccattattt tttgacataa aaagagcatt tgacaaagca tttgatatat
    49261 accaatttat tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt
    49321 aaatgtttcc agataaaaaa caaaaaggag gatagttata ccttttagga tagcgccagg
    49381 tctgtgtaaa cagatgacga ggagagggtt tatcgaaaga ttcggcggat ggccctaggg
    49441 actgcactct acagaaaatc cacaaaaagt atctgcaaag ataaaacaaa aggattttcc
    49501 tgcagctgga agcattcata cgaattcatt atagaaagta ggatgttttt tatgtgcgga
    49561 attgttggaa tgattggttt aaaaaatgta acaccaggat tgattgacgg tctagaaaaa
    49621 ttagaatacc gtggttatga ttcagctggt atctttgtct cagatggcac aactgattat
    49681 ctggtcaaag cacaaggcag aattcaaaat ttgaaaaaca aaataaatat tgatactaca
    49741 ggaaatatag gtattggtca tacacgttgg gcaactcacg ggcaaccttc tgaagaaaac
    49801 gcccatccac atacttccca aagtggacgc ttcgtgctcg ttcacaacgg cgtaatcgaa
    49861 aactttgaag aacttaaaat tgcttattta gcaaatgacc actttattgg tgagaccgat
    49921 actgagatta tcgctcatct aattgaaact ttcgcgaagg atactacaac gcaagaggcg
    49981 tttttgaaag ctttgcgggt catcaaaggt tcttacgctt tcgctttgat tgatcggaca
    50041 gcacctgatg ttatttacgt cgctaaaaat aaaagtccac tattaatcgg actcggcgat
    50101 ggattcaatg tgattgccag cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt
    50161 gagattgaag ataaagagat ggttactgtc acatcagaaa aagttattat tcaaaatttt
    50221 gcaggtgaca tcgtagaacg ttcttcattc gaagcacaag tagatgcttc agatatcgag
    50281 aaaggtacct atccttttta tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt
    50341 cgtatcgctc aaaaatacgt aacagaagac aatcgtgtct ctcttgatca aaagctggtc
    50401 gatacattgt ctgatagcga tcgaatctat atcgttgctt gcggaactag ttaccatgcg
    50461 gggttagcag gtaaacagac tttggaaaaa ttgacacaga tccctgttga ggttcattta
    50521 gccagtgaat ttggctataa cacacctctt ctttcaaaaa aaccattctt tattttcttg
    50581 agtcaaagtg gcgaaacagc agatagccgc caagttctgg taaaaatcaa ccgtctcggc
    50641 tacccttcac ttacgattac aaatgttgct ggttctactc tttcgcggga agcatccttt
    50701 acactgctat tgcatgctgg acccgaaatc gcagtagcat caacgaaagc ttatactgca
    50761 caaatagctg tactaacttt attagccaaa gctatcggtg ataaaaaaga atttgaaact
    50821 tctcttgtat tcgatatcac tcatgaattg agtttagttg ctaatgcgat ggaatcggtc
    50881 attgcccaaa aagattattt agaagagttg gcagaagtat atctcagcga tacaagaaat
    50941 gccttttaca ttggacgtgg cgctgactac aacgtttctc ttgaagctgc tttgaaattg
    51001 aaagaaatca gttatatcca ggcagaaggt tttgccgctg gcgaattgaa acatggaacg
    51061 attgctttga tcgaagaagg aacaccagtg atcggaatca tcagtgaaga agtgactggt
    51121 gcgcatactc gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac attagttatt
    51181 gtctcagagg gattagaaaa ggaaagtgac cagctggtgc taccaactgt ccacccttat
    51241 ctaactactc taacgacagt cgtaccgact caattacttg catattacgc aacgctatta
    51301 cggggattcg acgttgacaa accacgtaat ttagcgaaat cagtgacagt agaataataa
    51361 aagaaca
//
LOCUS       DAGCXG010000008        50408 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGCXG010000008 DAGCXG010000000
VERSION     DAGCXG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.008"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..764)
                     /locus_tag="MB022_000485"
     CDS             complement(<1..764)
                     /locus_tag="MB022_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBT4465512.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEF"
     gene            1410..4217
                     /locus_tag="MB022_000486"
     CDS             1410..4217
                     /locus_tag="MB022_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4465513.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            4408..4899
                     /locus_tag="MB022_000487"
     CDS             4408..4899
                     /locus_tag="MB022_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4465514.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5002..5991
                     /gene="manX"
                     /locus_tag="MB022_000488"
     CDS             5002..5991
                     /gene="manX"
                     /locus_tag="MB022_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBT4465515.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6024..6827
                     /locus_tag="MB022_000489"
     CDS             6024..6827
                     /locus_tag="MB022_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBT4465516.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            6848..7762
                     /locus_tag="MB022_000490"
     CDS             6848..7762
                     /locus_tag="MB022_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBT4465517.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            7845..8219
                     /locus_tag="MB022_000491"
     CDS             7845..8219
                     /locus_tag="MB022_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBT4465518.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8358..8603
                     /locus_tag="MB022_000492"
     CDS             8358..8603
                     /locus_tag="MB022_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4465519.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8775..9839
                     /locus_tag="MB022_000493"
     CDS             8775..9839
                     /locus_tag="MB022_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4465520.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            10085..10510
                     /locus_tag="MB022_000494"
     CDS             10085..10510
                     /locus_tag="MB022_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4465521.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10503..10952
                     /gene="nusB"
                     /locus_tag="MB022_000495"
     CDS             10503..10952
                     /gene="nusB"
                     /locus_tag="MB022_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBT4465522.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            11049..11903
                     /locus_tag="MB022_000496"
     CDS             11049..11903
                     /locus_tag="MB022_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBT4465523.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            11904..13241
                     /locus_tag="MB022_000497"
     CDS             11904..13241
                     /locus_tag="MB022_000497"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBT4465524.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            13246..13470
                     /locus_tag="MB022_000498"
     CDS             13246..13470
                     /locus_tag="MB022_000498"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBT4465525.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13472..14362
                     /locus_tag="MB022_000499"
     CDS             13472..14362
                     /locus_tag="MB022_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4465526.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14371..15189
                     /locus_tag="MB022_000500"
     CDS             14371..15189
                     /locus_tag="MB022_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBT4465527.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15296..15745
                     /locus_tag="MB022_000501"
     CDS             15296..15745
                     /locus_tag="MB022_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4465528.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15763..17445
                     /gene="recN"
                     /locus_tag="MB022_000502"
     CDS             15763..17445
                     /gene="recN"
                     /locus_tag="MB022_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBT4465529.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17500..17616)
                     /locus_tag="MB022_000503"
     CDS             complement(17500..17616)
                     /locus_tag="MB022_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBT4465530.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17689..18645)
                     /locus_tag="MB022_000504"
     CDS             complement(17689..18645)
                     /locus_tag="MB022_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4465531.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            18816..19184
                     /locus_tag="MB022_000505"
     CDS             18816..19184
                     /locus_tag="MB022_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBT4465532.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19354..19785
                     /gene="mraZ"
                     /locus_tag="MB022_000506"
     CDS             19354..19785
                     /gene="mraZ"
                     /locus_tag="MB022_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBT4465533.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            19806..20765
                     /gene="rsmH"
                     /locus_tag="MB022_000507"
     CDS             19806..20765
                     /gene="rsmH"
                     /locus_tag="MB022_000507"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBT4465534.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20770..21171
                     /gene="ftsL"
                     /locus_tag="MB022_000508"
     CDS             20770..21171
                     /gene="ftsL"
                     /locus_tag="MB022_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBT4465535.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            21172..23364
                     /locus_tag="MB022_000509"
     CDS             21172..23364
                     /locus_tag="MB022_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4465536.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23390..24352
                     /locus_tag="MB022_000510"
     CDS             23390..24352
                     /locus_tag="MB022_000510"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBT4465537.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24366..25727
                     /locus_tag="MB022_000511"
     CDS             24366..25727
                     /locus_tag="MB022_000511"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBT4465538.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25753..26841
                     /gene="murG"
                     /locus_tag="MB022_000512"
     CDS             25753..26841
                     /gene="murG"
                     /locus_tag="MB022_000512"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBT4465539.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            26856..28076
                     /locus_tag="MB022_000513"
     CDS             26856..28076
                     /locus_tag="MB022_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBT4465540.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            28232..29557
                     /gene="ftsA"
                     /locus_tag="MB022_000514"
     CDS             28232..29557
                     /gene="ftsA"
                     /locus_tag="MB022_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBT4465541.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29580..30821
                     /gene="ftsZ"
                     /locus_tag="MB022_000515"
     CDS             29580..30821
                     /gene="ftsZ"
                     /locus_tag="MB022_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBT4465542.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            30838..31515
                     /locus_tag="MB022_000516"
     CDS             30838..31515
                     /locus_tag="MB022_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBT4465543.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31529..32128
                     /locus_tag="MB022_000517"
     CDS             31529..32128
                     /locus_tag="MB022_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBT4465544.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            32143..32430
                     /locus_tag="MB022_000518"
     CDS             32143..32430
                     /locus_tag="MB022_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBT4465545.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32479..33261
                     /locus_tag="MB022_000519"
     CDS             32479..33261
                     /locus_tag="MB022_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBT4465546.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            33282..33989
                     /locus_tag="MB022_000520"
     CDS             33282..33989
                     /locus_tag="MB022_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBT4465547.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSAEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            34251..37049
                     /gene="ileS"
                     /locus_tag="MB022_000521"
     CDS             34251..37049
                     /gene="ileS"
                     /locus_tag="MB022_000521"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBT4465548.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(37098..38624)
                     /locus_tag="MB022_000522"
     CDS             complement(37098..38624)
                     /locus_tag="MB022_000522"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBT4465549.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38639..39286)
                     /locus_tag="MB022_000523"
     CDS             complement(38639..39286)
                     /locus_tag="MB022_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBT4465550.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39470..39799)
                     /locus_tag="MB022_000524"
     CDS             complement(39470..39799)
                     /locus_tag="MB022_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBT4465551.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            39976..40704
                     /gene="sfsA"
                     /locus_tag="MB022_000525"
     CDS             39976..40704
                     /gene="sfsA"
                     /locus_tag="MB022_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBT4465552.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40720..41733
                     /locus_tag="MB022_000526"
     CDS             40720..41733
                     /locus_tag="MB022_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4465553.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41733..43010
                     /locus_tag="MB022_000527"
     CDS             41733..43010
                     /locus_tag="MB022_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4465554.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            43073..45775
                     /locus_tag="MB022_000528"
     CDS             43073..45775
                     /locus_tag="MB022_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4465555.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            45927..46244
                     /locus_tag="MB022_000529"
     CDS             45927..46244
                     /locus_tag="MB022_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4465556.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            46274..46594
                     /locus_tag="MB022_000530"
     CDS             46274..46594
                     /locus_tag="MB022_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4465557.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46702..48162
                     /locus_tag="MB022_000531"
     CDS             46702..48162
                     /locus_tag="MB022_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4465558.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            48230..48451
                     /locus_tag="MB022_000532"
     CDS             48230..48451
                     /locus_tag="MB022_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465559.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48482..48664
                     /locus_tag="MB022_000533"
     CDS             48482..48664
                     /locus_tag="MB022_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBT4465560.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48664..49077
                     /locus_tag="MB022_000534"
     CDS             48664..49077
                     /locus_tag="MB022_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBT4465561.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            49200..50381
                     /locus_tag="MB022_000535"
     CDS             49200..50381
                     /locus_tag="MB022_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4465562.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        16797 a         8514 c        10824 g        14273 t
ORIGIN      
        1 ttaaattctt cttctgtaat gattccatca tccaatagtt ctttatattt tcttatttct
       61 gaagctgagg aaattgttga tgtattggaa tttgtagcag aaatatttct tctttttaaa
      121 ttataaataa tttcgtttaa agaatcactt atcctatcaa cagtatcttt atcaactgca
      181 acattcaata ctttaccaag agtatcaata gttaatgtac ccaacagcac tcctcttttt
      241 ttactaatat tatttaaatt gtctaatata atactttgta cattattgcc tatcattttt
      301 tgttgagcta caattaaacg tttattagtt aatgcgtatg cataattatt gtcatgctta
      361 gttgatgaga tataattatg caatcctaca aagcagaaga ttacgtattc gtcactgtta
      421 agttgatctg atattaagtt aaaatgatga gttgtccatt ttttagtcat tcctttaccg
      481 tagccgtatt taagacaaaa ttctaccatc tcttcagtgg ttctcattct atcattacct
      541 atatcatgat tgttttgtgc agtatgatca gatatgttta attttctttt ttctgatgga
      601 tccttatcta aatcttctaa attcaaagat cttattattt tataaggagt agtcataccg
      661 taaccagctt ttttcataca attgccgcaa acaatttgtt tatctttgat tttatatgaa
      721 gttaagccaa ttttacctcc gcactttgca cattgtttac tcacattagt tcctcctaga
      781 tatcagcttt ctactactat taacattcta tcaatagtga ccatactttg accatacttt
      841 ttagaaagaa tagaaaattc caagatattt aataatagaa gaaaagcact aacatcatgg
      901 gttcagaaga attgagagct tatcaggaaa cagtataatt gaagtgtctg tttatccagt
      961 agctcaagaa gcttaattga atcttatgat taaagaggtt gtgacaagac ttttgtcaca
     1021 gccttttttg tttataaaaa taaaagttgt agtggaggct acgaagttta ctttgtcttt
     1081 ttacacttaa tatttttttg ccaatttgga atcaggatgt tttgataact atcgttttca
     1141 attgatggaa gctatattgt ttgctatcag tgaaataatt atatggtaaa cagtgttctt
     1201 tttcttgttt ccattataaa aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt
     1261 ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg aaacagaaat
     1321 aaatacatag tattatgttt tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac
     1381 cattggcaaa acgcattgac cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa
     1441 caaaggcaga atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg ttagggattc
     1501 agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga
     1561 cagacggacg accagtacgt tacatatatc aaacactcgt tacgcacgta aagccagtta
     1621 caaaacatgt agtcagttac aaagaaacgc cggtctctga agaaaaaaat acactccaaa
     1681 tagatactaa ggatatcttt gcaaaaatca ttggagctaa cggcagcatg aaaaattcag
     1741 tggaacaggc aaaagctgcg attttgtatc cgccaagagg attaaattgt ttgattacag
     1801 gacctaccgg atcaggaaag acttattttg cacacgcgat gtatcatttt gcaaaagcgc
     1861 atcatgtaat agatgaggaa acggaactga ttgttttcaa ttgtgctgat tatgccaata
     1921 accctgagct tttgatgagt catttatttg ggtacgcaaa aggtgctttt acaggcgcag
     1981 atgaagaaaa aatggggatt atcgatcaag cggatggcgg tatgctgttt ttggatgaga
     2041 ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta ctttatggat catggaactt
     2101 acagtcgtct tggggaaaca acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga
     2161 ccacagaaga cccaggttct tctctattag aaacttttgt tcggcgtatc ccaatcaata
     2221 tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa agtcgattta gtaaaaatca
     2281 tgattgccca cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa
     2341 aagcattgat cggcagtgtg acctatggaa atataggaca attgaagtca aatatccagt
     2401 tagtatgtgc acgaggattt ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg
     2461 atgatttgac agaagggatt cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa
     2521 tggcagagct ttctaaaata ttggagccaa agataactgt agcaccaaat gatacgatca
     2581 tgaaaatcca atcggactcc tatgagttac cttataatct atatgatatc atcggagata
     2641 aagcagcttt actgaagtcg gatggtttgg atcaagaagc aatcaatcat ttcatttcta
     2701 cagatatcaa catccatctg aagtctttct ataaagacca tggtttttct tttaatgcag
     2761 acagcaaatt agcagaattt gtagatccga aaatcataga agtgaccaat cagatttatg
     2821 taatggtcaa aagatcttta ccatatgagt ttcagcaaaa ctttatttat gctatgagct
     2881 tgcatatcag ttcttttttg aaaaaaatca acttaggaga agaacggcat acgaatgata
     2941 acatccgtga gatggctttt gattttccag atgaatatgc tgttgcgaaa gaagtgcgta
     3001 gttatatcga aacgtatttt caagtggaga ttccagaaag tgaagactat tacttgaccg
     3061 ttctactcgt ctcgttacgg gcaaatcaag caagcggcag aatcggtgta gtcgttgcag
     3121 ctcacggaaa cagcacagca agcagtatgg tccaagttgt ccaacagttg ttagatgcag
     3181 agaacgtacg agcagtagac atgcctttgg atatggatcc gaaaacagca ttggaccgaa
     3241 ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt catactctta gtagatatgg
     3301 ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac tggaattcca gtacgtactg
     3361 ttgatatggt aacaacttct ctagttttgg aaactgtacg aaaggtctca gtattaggga
     3421 cagatctaga tatgttgcat gaatcattga aaaattttcg aggttacgca gaaatatctt
     3481 ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt ttggctatct
     3541 gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca
     3601 agcgtcaaga aacagaatta gaagttctag ctttgtcgat tgtggaagtg aaagaagaac
     3661 tgccagttat ccaaaaagaa tatcagatca tagctactac tgggattact gatttgaata
     3721 tccaagcccc atttattcct ttagaacgtt ttattgatca aaatatcgaa gtcattttgg
     3781 atcagttgct gatggaatca gaattggagg aaactgaatt catctcccta gatgaagagt
     3841 cagctaaaaa tacgtgtgtt gagtttatct ctgataactt catctttatt aatggtagta
     3901 agttaatcga tccaatgtgg caattttcta cacaaatctc tcagacgacg ggaatcggcg
     3961 atgaagaata tggattcaaa atcaacctag tgatgcacac agcaggaatg atcgaacgta
     4021 ttattcggaa tgaaccattg actgtggaag aaaacgaatt gacaaataca acgaatgatc
     4081 ctctttatag ccaattggca gcctcagttg ttctgttgga agatcagata aaagtaaaag
     4141 tgccgataga agaaatgtat tatctgctac gtctagtcca taatcagtta gataaaaaag
     4201 aatacactgt gccatgaata cacaaaaacg atacactttt atagtgtatc gtttttgtgt
     4261 attggttaag aatcctttaa attcaattag tgtatcgtaa aaaagttggc acgctttttg
     4321 cttatagtag tgtgtgctag gaggtggttg ttcacacaga aatgataggg gtttctgtga
     4381 agtagtgagg aagaaggagt tttgcagatg gatattcgat tggcacgtat agatgatcgt
     4441 ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga acgtatcatt
     4501 gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa agaagctgca
     4561 ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt
     4621 ggattattat cacgagcaaa agtgatgctt ttattcacca atccccaaga tgttgaagcg
     4681 gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa
     4741 ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac aagatattga ttcctttcgt
     4801 tacttgaaca gtcaagaagt ggagttagag attcgaaaag tacctgctga caggaaaatc
     4861 cttttgatgg aactactagc gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag
     4921 gtcaaataat tctgttgtaa tggttagcaa ttattaatat ttttgaattt gttacaacaa
     4981 aagtaaatag gaggtataaa tatggtaggg attatcctag caagtcatgg acaatttgct
     5041 gaaggaatct tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg
     5101 attttgaaac caagtgaagg tccagatgac ttgagagcca aattggaaga agcggttgct
     5161 tcttttgata atcaagacga agtgttattc ttagttgatt tatggggcgg aacaccgttc
     5221 aatcaatcaa acacattatt cgaagagcac aaagataaat gggcaatcgt cagcggtttg
     5281 aatttaccaa tgttgattga agcctacgca tcacgctttt caatggaatc tgctcatgaa
     5341 attgcagcac acatcattga aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta
     5401 gaaccggcag aagcaccgaa agcagcagtt gaagacgcac aacctaaagg agcacttcca
     5461 gaaggaactg tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt
     5521 ctgttacatg gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc
     5581 gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga gactaatcga acaagctgct
     5641 cctccaggtg taaaagcaaa tgttattcct attagcaaaa tgatcgaagt agcaaaagac
     5701 cctcgttttg gaaataccaa agcattgctt ttatttgaaa atcctgaaga cgtattgaca
     5761 gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc
     5821 ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag aagacgttga agcgtttgag
     5881 aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga ttcacgcgat
     5941 aacatggatg atattttgaa aaaagcaaaa gctgaattag caaaagcgta ataaataata
     6001 ttaaaattta taggaggctt atcatgtcta ttatttcaat aattttagtc gtattagttg
     6061 cctttctagc tggtatggaa ggtattcttg atgaatttca atttcatcaa ccactagtgg
     6121 catgtacatt gatcggttta gttaccggta acctcgaagc tggtatcgta cttggcggta
     6181 cgcttcagat gatcgcttta ggttgggcaa atatcggtgc cgctgtagca ccagatgctg
     6241 cattagcatc agtagcatca gcaattattt tagttttagg tggacaaggt gttaaaggtg
     6301 ttccttctgc gatcgcgatt gccgttcctt tggctgttgc agggttgttc ttaacaatga
     6361 tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga tgcggctgct gaagaaggaa
     6421 acatccgtaa ggttgaaatg tggcacatca ttgctgtatg tttacaaggg gttcgtatcg
     6481 cggtcccagc agcagcgttg ctatttatcc cagcacaaac tgtgcaatca ttccttgaat
     6541 caatgccagc atggttaaca gatggtatgg ctatcggtgg gggaatggtc gtagctgtag
     6601 gttacgcttt ggtaatcaac atgatggcta caaaagaagt atggccattc ttcgttattg
     6661 gtttcgttgt agctgcaatt tctcaattaa cattgattgc tctaggtgct ttaggtgttg
     6721 cattagctct tatttacttg aacctttcta aaatgggtgg ttcatcaaat aatggaggcg
     6781 gaagcaactc tggtgaccct ttaggcgaca ttttgaatga ctattaatct tgaaggagga
     6841 aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg
     6901 gcgctctacc ttcattcaag gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc
     6961 attttctatg attccagcaa tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc
     7021 attaaaacgt cacttggaat tctttaatac acacccatac attgcttctc caattttagg
     7081 ggttacattg gctctggaag aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat
     7141 tcaaggggtt aaagtaggta tgatgggtcc tttagctggt gttggtgacc cagtgttctg
     7201 gtttacagta cgtccaatgc taggtgcact aggtgcttca ttagctatgg gaggaaatat
     7261 ccttggacca atcatcttct tcttaggttg gaacattatt cgttgggcat tcatgtggta
     7321 tacacaagaa tttggctaca aagctggttc taaaattact gatgaccttt ctggcggatt
     7381 gttacaagac gtaacaaaag gtgcttcaat tttagggatg ttcgtattag ctgcccttgt
     7441 acaaagatgg gtatcaatca aattcttacc agttgtatca actgtaaaat tagataaagg
     7501 tgcttacatc gaatgggata aattgccttc aggtggtgaa ggaatcaaga gtgcatttga
     7561 acaagttaat aatggcttag ccctttctcc tgaaaaggta acaacattac aagacaactt
     7621 agaccaattg attcctggtt tagctgcatt aggattaaca ttgttatgta tgtggttgct
     7681 taagaaaaag gtttctccaa tcgttatcat ccttggttta ttcgtagtcg gtgttgttgg
     7741 acacgtcatc ggacttttat aatccataaa aatatagctc gggcgttgtc ccgagctttt
     7801 tttatatagt atactgttca taaacaaata tctggaggga gaaaatggtt caatcaatca
     7861 atacaaaagt agacttggta attgatgcta cttcgtttac gggtctgaca gattacgggc
     7921 aaatcatgat aggtgataat ggttttgagt tttaccatgc gcgagataga cgcaaatata
     7981 tccaaattcc atgggaagaa gtggattatg tactagcatc agtcatgcta aagggaaaat
     8041 ggattcctcg atatgccatt caaacgaaaa aaaatggtac atacacattt tcttccaaag
     8101 aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga tgaagataga atggtacaat
     8161 cattgagttt ctttgatgta gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa
     8221 aaataaagaa aacttctaag aggaggtttt ctttattttt ttactttgca gaagaaaata
     8281 tgaaatagca aaaccaatta tttgtcccct ttaaatccca tatgctaaaa ttgattagca
     8341 aagaaggagg ttatattatg agttggttat ggacattgat tgttggagcc gtgatcggag
     8401 caattgcagg tgcgatcacc agtaaaggaa attcgatggg ctgtgtaacg aatatcattg
     8461 ctggactagt cggttcaagt attggacaag cgattttcgg cgaatgggga ccaaaagctg
     8521 caggtatggc acttattcca tctattttag gcgcagtgat tttggtagca gttgtctcat
     8581 ttttctttgg aaaacgatca taagttttta caaaatagta aactaaaagg agttgcagcg
     8641 agactgtgac tcctttttgg ctttatttat ttaaaataaa aagaagtttt ccggaaattt
     8701 ttatttaact gtcgtgtacg aagaaaagtt tgctataatg gaaaaaagcg tgcaaagaaa
     8761 gagggagttt cattatgatg ctacgtgtag aaaaattgcg caaaaaaatg caagaagaaa
     8821 atttggattc atttttggtt accagtccgt acaatcttcg ttacttaacg aactttacag
     8881 gtacgacagg attagctgtt attactttgg aaaaagcatt ttttattact gattttcgtt
     8941 ataccgagca ggcggcagcg caggctcaag ggtttgaaat tattaaaaat gtcggaccta
     9001 tttttgatga ggtagcaaac ttagtacgca aagaagaact gcactcatta gcttttgagg
     9061 aaacaacagt ctcattttta gaatattcgg ttttagaaga aatcatcgat gcggaattag
     9121 tacctgtttc acagatgatc gaagaactca gagaagtgaa agatgaagaa gaaatcgcta
     9181 ttatcgaaaa agcatgtcat attgcggata tggcatatga tcacatcctg aaaatgatcc
     9241 aaccgggcat gactgagatt gaagtcgcaa atcaactaga cttttatatg cgttctcttg
     9301 gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg attgcgttcg gctatgccgc
     9361 atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt gatcacattg gactttggtt
     9421 gctattacga agggtacgtt tccgatatga cacgtacatt cgctattggt gatcctgggg
     9481 aaaaattaaa agaaatttat caaatagtct tagatgctca attagcagtt attgatgctg
     9541 caaaaccagg aatgactggt gtccaattag atgcagttgc tagagattat atttcaaaac
     9601 atggttacgg ggaagcattt ggacattcta caggacatgg gatcggttta gagatccacg
     9661 aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt accaggaaat gtgatcactg
     9721 atgaaccagg gatctactta ccaggaatcg gtggagtacg tattgaggat gacttgttga
     9781 tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa agaattgatc atcttatagt
     9841 ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac agactttgtt atagaaaatc
     9901 actcgattta atgatacact atagaagaac ttttaagctg taagagataa taaagttggg
     9961 tgttttctcc aattcatagc agtttgcagc tgatcggcgg cagttttctt accacgtgat
    10021 cactatgccg catcatagag aattcggatg tatctcgtgc ttaggcaaag ggaggaataa
    10081 ataaatggca gatgaaaaaa atttgatttt aaacgcaaac caagatttag gagagatcgt
    10141 gatcgctcct gaagtcatcg aagtaattat cgggattgca gcttctaaag tagaaggcgt
    10201 atacggaatg cgcggaactt ttgccagcaa tgtcactgaa ttattaggac gtgcagctca
    10261 cggaaaaggt gtttacttag caaacgagga agatggcttg aaagtagatc tttactgtta
    10321 tctagaatat ggtgtttcag ttcctaaagt agcaatggaa atgcaggaac gggtcagcca
    10381 gcaagtcctt ttcatgacgg atatcgattt atctgaagtc aatatccatg tagtagctgt
    10441 tgttcctgaa aaattagaac aaccaacaat cgaagaactt tttgaagacg aagaggaaga
    10501 gaatgaataa agaattatca agacacgaga ttcgggaaat ggcattgcag gctttatttc
    10561 cattagactt caatgcagac ctaacaaaag aagatgcgat tttcaacgca atcgaattag
    10621 accaccgcga catgatcaac gaagatgaat cagagtttgt tcctgtttat cttgatacgc
    10681 tagttggcgg tgtctgtgca aaaaaagatg aattggataa agtgattgaa aaacatttaa
    10741 aaaacaattg gcacatcaat cggatctcaa aaatggattt ggtgatcttg cgtatcgcga
    10801 tttttgaaat gacttatgtg acagatgtac cagcggctgt tgctttaaat gaagcaatcg
    10861 agttggcaaa aacatttagt gatgaccgtt cacgtaaatt cgtcaacggt gttttatcca
    10921 atgtattgaa agaattagaa gctggggcgt aagtcctagc ttttttctat gaatcatcat
    10981 aaacctgtta taaatcggga aaattctatg gtagaatgaa aacacaaaaa atatctggga
    11041 gtggttagat ggcagaattg attaacggaa aagaattagc tgaaaaaatg caggcagaga
    11101 ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca tcccggatta gtcgttttat
    11161 tggtagggga aaatccagcc agccaaatct atgtaagaaa taaagaacgt gtagcaaaag
    11221 aaatcggaat ccattcattg gtagagcgtt atcctgagac gatttctgaa gacgaactac
    11281 ttgcagaaat tgaaaaatac aatcaagatc ctagttttca tggtatattg gtccaattac
    11341 cattgccaaa gcatatagat gaagaaaaga tcctgttagc tatccgccct gaaaaagacg
    11401 tagacggttt tcatccgatg aatttaggca gactccttgc gggtaaaccg gacaagattc
    11461 cttgtactcc atacggcatc atgaaaatgt ttgaagctta ttctatcgat ccggcgggta
    11521 aacgagcagt agttatcgga cgaagcaata tcgtcggtaa accgatggct cagctactct
    11581 tgatggcaga tgcaactgtt actgttgctc attctaaaac cgaaaatctg gctgaactgg
    11641 cacaagaagc agatatttta gtcgtagcaa taggacgtgg acattttgtc acaaaagaat
    11701 tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa tcgtgatgct aacggtaagc
    11761 tgatcggtga tgtgaaattt gatgaagtgg agcctattgc aggctggatc actcctgtgc
    11821 caaagggtgt agggccaatg actatcacga tgctgatgta tcaaaccgta aaatctgccg
    11881 aagcttttgg agcaggtgaa tagatggcac aagaatattt aactgtcact acattaacga
    11941 agtatttgaa aagaaaattt gatgccgatc cttatttaga acgcgtatat ttaacaggcg
    12001 aaatatcgaa tttccgttta cgtgccaatg cccatcaata tttcagctta aaagatgacc
    12061 atgcaaaaat atctgctatt atgttcaaag gagcattcca aaaattacga ttccagccaa
    12121 aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt atatgaagcg ggaggttctt
    12181 atcaaatcta tgtagagcac atggagcctg atggaatagg tgctttatat caagaattgg
    12241 aagaacgaaa agaaaagctg atgaaggaag gcctttttca agggcctaaa aaaatgctgc
    12301 cacgtttccc aaagcgaatc gcggtcctta ccagtccaag cggagcggtt atccgcgata
    12361 tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt ggttttgttc ccaacagttg
    12421 tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat ccagagagta gaagagatgg
    12481 gcaatttcga tacaatgatc atcggtcgtg ggggtggttc gattgaggat ttatggccgt
    12541 ttaatgaaga gcgggttgct cgggcgattt atcaagctaa tacgccggtg atttcatctg
    12601 taggacatga aacagatacc acgatcgctg atttagtagc agatgttcgg gctgccactc
    12661 caacagcagc tgcagaatta gctgttcctg ttttgtcaga agagttgatg cgcattcaag
    12721 aacggcaagc tagattgcag caggcgtttg tacgccagat ccagcgcaaa caagaacggt
    12781 tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga aagattgtat gaaggtcaga
    12841 ccatcaaatt agaccagttg aaacagcgat tatatcaagc tacacagcaa atttatcatg
    12901 cgaaagaaaa acaaacgatc gcattaagcc accaattgga acaagtggct ccgctttatc
    12961 gagttaaagc agctcgccaa gaaactgctt atctagaaaa acgcttagaa gagaaaatcc
    13021 aacaatatat gaaacaacag cagcagcgtt ttgctcaagc tgttcaatct cttgatttgt
    13081 tgagtccatt gaaaatcatg ggaagaggct acagctatac gacgttaaat gatcaagtag
    13141 taaaaagtgt caccgagatt catcaagggg atttgctgac gatccattat caagatggaa
    13201 tcgttaaagg acaagtacaa gagatagaca aaggagaata aaaatatgga aaatccaact
    13261 tttgaagagt cactgcaaga attagaaaaa attgtcatgc agttagaaca aggagacgtt
    13321 ccattagaat cagctctaga ttcgtttaaa cgaggaatgg aattaagcaa acattgccaa
    13381 gatacattag ctaaagcaga agagaccttg acgaaaatga tgacagaatc aaatgaagaa
    13441 gttgattttg acggaaacga ggaaagctga gatgagatta actgactttt cagctattca
    13501 tctacctgct gtagaaaaag aaatcttgtc ttttttagat gagcatacaa cggatagagg
    13561 actctatgat gcgatgagtt attcagtgaa agcaggcggg aaaagaatac gtcccttatt
    13621 acttttaacg gccgttgctt cctttgatga gcctattgat gttcccgtct atcaagtagc
    13681 agctgcttta gaaatggtgc atacgtattc cttgatccac gatgatttgc ctgcgatgga
    13741 caatgatgat cttcgtagag gaaaacctac caatcataaa gtattcggcg aagcattagc
    13801 tatattggcc ggagatggtt tgcttacagg agcattccag ctaatcagta tggcacactt
    13861 aggtaattct cctaagcttc tgctgcttca gcaattagct gtttgtgcgg gatcacaagg
    13921 tatggttgct gggcaagctg cagatatcga gggcgaatcg aaaaagcttt ctttagaaga
    13981 attagcattt attcatgaaa gaaagacagg gcatttgatt cgttatgcct tgctggctgg
    14041 tggcatatta gcgaagcaac ctgaagaaat cttattgcta cttcaacgat tagccgaaca
    14101 tttaggttta gcttttcaga taagagatga tctactcgat gtgattggta caacgaaaac
    14161 attagggaaa acagctggta aagatgaacg tatggaaaaa aatacttacc cgcgtttatt
    14221 aggcctagaa aagacacgtg aggcgttgga gattgagctg ttatccgcca acaagatcat
    14281 agacaaatta gaagaggacg ttttttcttt tgatggcggg ctgatgcgcc agatgatcaa
    14341 gcaatttgat gtggagaaat gagggaaaga atggaaaaag aacgcgtaga tgtattagcg
    14401 gtcaaacaag gattatttga aacaagagaa caagccaaac gttcagtgat ggctggtttg
    14461 atttacaacg aaaaaaacga gcggtttgat aaaccgggag aaaaaattcc agtgagtagt
    14521 gaattaagag tcaaaggtaa aaaacttcct tatgtttctc gagggggatt aaaattagaa
    14581 aaagcactca aagcatttga tctatctgtt gaaggaaaaa cattgttaga tattggtgca
    14641 tccactggcg gatttactga tgcagctttg caaaatggcg caaaaatgag ttatgcttta
    14701 gacgttggct acaatcagct agcttggaaa ataagacaag atcctagagt cgtcgtaatg
    14761 gaaagagtga attttcgtta tgcaaaaccc gaagacttta ccttaggaat tccagaagtt
    14821 gctgttatcg atgtttcttt tatttcacta aaactgatgc tgcctccatt acatgctatt
    14881 ttaaaagaag agggagaagt gattgcttta atcaaacccc aattcgaagc aggcagagaa
    14941 gctgtaggga aaaacggaat tgttcgtgac cctcagaccc atcaaaaagt tttagaagac
    15001 attttgtcat ttgcggcaca aggtggatat gatgtgctgg aattaagtta ttctccgatc
    15061 acggggggag aaggaaatat cgagttcttg gctcacttga ggaaagtacc cgaaagcgga
    15121 acaatcaatt cagctattaa tatggcagaa gtggtttcaa atgcacacga acagtttgac
    15181 cataaataaa ttgtaactgt gaaaatcaag tgagaagtat gcggattctt ctttattata
    15241 ccaaggaaat aagctatcat tgattaagaa gaatatgcta aggagcgaag acgtaatgag
    15301 aaaaaaagat cggcatcgtt tgattactcg attgttgagt gagcaagata tccgaaagca
    15361 ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct gttactcaag ctacgatttc
    15421 cagagatata aaggaactga aactgatcaa ggtaccggct tcagatggcg gctaccgtta
    15481 tagcatacca gctgaaacaa acgaagatgt aaatactaaa ttggaaaaac tattaaaaga
    15541 cgctttcgtg gcagttgacc aaatggaaaa atttgttatt ttgaaaacat tgccgggaaa
    15601 tgcttcggca gcagcaaatt tgattgataa acgttacaag aaagaattat tttcgattat
    15661 caatgatgac gataatgttt tgatgattac acgaactgaa gaccaggcag ttttattgaa
    15721 gaaagacttc ctgcattacc tttaagttga ggagcgaatg tgatgctaca agaaatcagt
    15781 attactaact ttgcgattat tccggaattg cggctttctt ttcacgaagg aatgaccgcg
    15841 ctgaccggag agactggtgc gggtaaatcc attattatcg atgctttagg tctgttagca
    15901 ggaggaagag gttcaagcga ttatatacgt caaggagcag aaaaatgtgt gcttgaaggg
    15961 ttgtttgaac taccaaaaca agaaggattc tctgaactga tggttgaatt agggatcgaa
    16021 acagatgagg acaatctgat tgttcgacgg gatatgtcct taactggaaa aaatgtttgt
    16081 cgagtgaatg gtcatattat cacattagct aatctgagaa gaatcggcag ttatttagta
    16141 gatatccaag gacaaaacga acatcaggaa ctgctccagc ctgaatcaca tcttgcttta
    16201 ttggatcgtt ttggagatgt agctttccaa cagaagaaaa agagttatca gcaagaatat
    16261 atctcttatc gtgagctgga aaaaagggta cgaaaagtgc agcaaaatga aaaatcctat
    16321 gttcagagaa tagatatgct ccatttccag caagaagaaa tagctgctgc tgatttgcaa
    16381 gtcggagaag aagagaaatt aaaagaagaa agagaaaaac ttagcaatta tcaaaaaatt
    16441 gctgatggat tagctgcagg ttacggagca ctgacgaata atgaacaaaa cagtgtagat
    16501 ggtgttggct tggccgtttc agaactgcaa gggatcgccc atctagatgt ggagtatgaa
    16561 gcaatttatg aaaatatcca aagtgcttac tatttgctgc aagatgcaat cggcgatatg
    16621 agtcgtcaga ttgatttatt ggagttagat gagagtcgtt tagaagaagt tacgcaacgt
    16681 ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat ccattgagtc aatattggct
    16741 tactatgacg aaatcaccga agagctagct tcttctgatt tttcagaagg tcaattggac
    16801 aaaatgaaag aggaactaga gcaaaaagag cttctacttc agcaacaagc cgcagatctt
    16861 catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat ccattctgca tgaattgaaa
    16921 agtctgtata tggaaaacac tgagtttgag gtacgtttct taaaagaaga aaatagacaa
    16981 ttgaatcatg atggatttga ccaaatcgaa ttttacatta cgaccaatcc aggagagcca
    17041 ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt ctcggatgtt gcttgctttg
    17101 aaaactattt tttcttctga gcaaggtgtg acaagtatta tttttgatga agtggataca
    17161 ggtgtgagcg gaagagttgc acaggccatc gcagataaaa tttctcaaat atcgaaatac
    17221 tctcaagttt tgtgcatcac tcatctgcct caagttgctg cagtcgccga ctatcaatat
    17281 tatattgtga aagcagtgat tgatggacgt acgcaaactt ctgtttcaga gttgaaaaca
    17341 aaagaaagag aagaagaaat tgcacgtatg ctggctggaa gtgagattac aaaactcaca
    17401 ctcgaacatg caaaagaact attgaaactc gcaaaaagga cctgagccac ctcgcagtgg
    17461 ttcaggtcct tttcagcagc tgatagctat attttatttt tacatgacac tcattacagc
    17521 agtcaataca acagatccaa tcgttaaaat cagcattgtc caaatgacaa attttgttac
    17581 tttagcaaaa gtacttgttt gttttttttc acgcattgtt ttcaacctcg tcattaatta
    17641 gttgattcaa gtgtacaatc ttttttccat ttccacaaga gggctttttt ataaaaactt
    17701 cccttttttt aaataatagg tagttacgat acaaataaat aatgttaata gaactagcca
    17761 tataaaggca tcatcccttt cagcaatagg taacttaatg ttcattccat agatactgcc
    17821 gacgattgtc ggtatagtta aaataatcgt taaagaagtt aaaattttca taatattatt
    17881 cagattattc gatacgattg ctgaaaaagt ctgattcatt tgttctacta gttttaattg
    17941 aatgcgagaa gtagtcattg cctgctctgt ttccaccaat acatcacgta aatgattttt
    18001 caaagcatga ttttcttgga aaagtttatt ttgcgctagt tctttcaatg catctaaatt
    18061 agcagaagtc gcttctctga aataaacgag gcttttttgg atatccatga tttgataaag
    18121 ttgtttgttt tcagtggata cttgaagttt ggcttccaaa gtatcgcatt gagattgaag
    18181 cgtagctaaa taactgttgt aagagagtgt gacctgccaa atgaattgaa agaagatatt
    18241 gagattaggc gaaatatcac tttttggaaa ggtctggcta aagactttct ttaaaaacag
    18301 tggttcatga ttcgtaattg tgataagctt ctttttgggc gtcaaaataa tagagagcgg
    18361 gtatgtatcc acttgaaaat aaccgctagg acttgtttta gcaaaaggat attgtaacaa
    18421 taataacgct ggctttgtca actttgattg attgaattct tctgtacggg aattttctgc
    18481 atcgtccaat atggaagtga tgtaatctga cggaatttca aattgatgtg tcagctgatg
    18541 aatctcttct tgattaggac gttctacaca ataccagatc gtttggtcag tttcctttcc
    18601 ggtattacgc agtttctcat tatctaatgt aaaatatttg atcatgattt tcctcctgtt
    18661 gcctgtttct tttattataa cggataggga attggaatac atggaatgtg tttgtaagat
    18721 atagaatata actgcataaa tttaaataaa atgaaacagt tgtgtaaagt atatacagta
    18781 tacttgaagc aacgataaaa aaggagttta cgttcatggg cacattcaca ccacttatct
    18841 tgttttggta tatttacccg gtaatcgttt tgtttggttg ccaatttctt gtaaaattat
    18901 tttcgttaaa tcgtcgtttt aaaataaaag cccctgattt ggcgattcct tttttgtggg
    18961 gcggtatgca tgcattgtct agaaatacag ggacaatttc tatcttaccg tttttcttta
    19021 tttctgtttt gctaatggga atttgtattg ctgttttcca agcatattat tatgaagaaa
    19081 ttatttatcc gcgttatttc aagatgactt ggcgtttggt ttttctgttg acaatcgtct
    19141 tgtacgctgt cttgatcatt gtgaacatac tttcttacct atgaaaaaaa tagaaaagct
    19201 gtaaaaagtc ttttaaaagg ctttttgcag cttgattttt aaaaaaatgt ttatgaataa
    19261 aaaaagttgt tcccggattt cccagaaaag gtggtagaaa gtgggggatt gtggtagact
    19321 aattttagtt agtggaggaa tggggggctt tcaatgttga tgggtgaatt ccaacataat
    19381 attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc gtgaagaact tggcgaaaag
    19441 tttgttctga cacgcggatt ggatggctgt cttttcggct atccgatgag cgaatgggaa
    19501 aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa aagacgcacg tacttttgtg
    19561 cgattcttct attcggctgc cactgaatgc gaacttgata aacaaggacg aatcaatata
    19621 cccagcacgt taagaaatta tgcagctttg acaaaaggtt gcgtagtcat cggtgtatct
    19681 aaccgaattg aaatttggga tgagacacga tggcaagatt tttctgcagc agcagaagag
    19741 aactttgacg aaattgctga aagtatgatt gatttcggac tataagaata ggaagaggct
    19801 gactaatgac tgaaacattt caacactata cagtcatgtt gaaagaaacg gtagatggat
    19861 tgaacatcaa accggatggc gtttatgtag actgtacact cggcggtgcc ggacatagtg
    19921 aatatttatt aagccaacta agttctaaag ggcatctgta tgcgtttgac caagatcaaa
    19981 aagcaatcga tcacgccaag ctgcgtttag cttcttatgt cgaacaaggt atggtcacat
    20041 ttatcaaagc gaacttcaga gaattagaag aacgcttgag tgagcatgta gataaggtag
    20101 acggcatttt gtatgatcta ggggtttcct ctcctcagct agatgaagca gaacgtggat
    20161 ttagttatca tcaagatgca ccgttggata tgcgcatgga tcagtcagct cctttatccg
    20221 cttatcacgt aattaatgaa tattcctacc atgaattagt aaagattttc ttccgctatg
    20281 gcgaggagaa attttccaaa caaattgctc gtgaaatcga acgagtacga aaaacaaaac
    20341 caatccaaac aactggtgag cttgtcgaaa tcattaaaca agtcatccca gctcccgcaa
    20401 gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc tgtgcggatt gcagtaaatg
    20461 acgaactcgg tgccgaagag gcctctttag aacaggcaat ccggttattg aaagtcaatg
    20521 gaagaatcag cgtaatcaca ttccattcat tggaagacag aattgtcaaa agtatgttta
    20581 aagaatatag tacggtacaa gatctaccac cgggacttcc tgtcatacct gacgaattcc
    20641 aaccagaact gaaagtcatt actcgtaaac caatccttcc ttcagaggaa gagctaacag
    20701 aaaacaatcg atcacgcagt gcaaagttac gtattgcaga aaaaataaga atgaaagagg
    20761 agtagaatga tggcagaatt aaagaaaatg caagattacg aatatgattt acctgaattg
    20821 gtggatcagc cggaacaaca aatgcctgat tcttcttcga atcaagaaat cattcttcca
    20881 caatcaccgg caaaacgatt aaagcagatt tctgctttgg aaaaaattgc tgtggcaagc
    20941 gtggcgattg ctattgttgc tttatgtgtt ttaacagtta tgctgcgcac aaatatcagc
    21001 ggagctgaaa aagaaatcac gtcaatacaa acagaaatca atgataaaaa gcaagaaaaa
    21061 acgagtttag aacaagaaaa aaacgaactt tctcgtactg aacggattaa acaaatcgca
    21121 gaagaaaaag gattgtctat taatgacgac aatttaagga aagtgaagta aatgagtctt
    21181 aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa atccgatgaa caatcgcaaa
    21241 aaagtaggta tcattttatt tgctacgagt attggattgt tctttttatt tgccgtccgt
    21301 ttctcgtata tcgttattgg aggtcatgtt gctggtacct cattagctga aaaaacaaaa
    21361 cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag gaacgattta tgatcgtaac
    21421 ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta aagcgatact ctcaaaaaca
    21481 tatacctctg gggataaaaa actgtatgtt gaagaaaaga attttgataa aatagcagaa
    21541 atcctgcata agaatctttc tatagacaaa aaagatgcac tgaatatttt agaggacggc
    21601 gcaaaaaaag agctgtatca agttgaattt ggtagctatg ggaaaaatat ctcccaagaa
    21661 accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg tcgctggtct ttattttgtc
    21721 gaccatcagg ctcggatgta tccaaatggc gtcttttctt ctcattttat tggatacgca
    21781 gttccagaca aagatgagaa tggcttagtt ggtaaactag gtttggagtc tgcttacaat
    21841 gatatcttga gcggtaaaga tggtaagatt atttatcaaa aagataattt tcaaaatcct
    21901 ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg gacaagatat ctacacgact
    21961 ttagacagca gactacaaag ttatttagaa acactgatgg atcaagtaaa tgaagaatat
    22021 cagccagaag aattgacagc agtcctgatg aaagcaaaaa ccggagaaat cttggcaatg
    22081 ggacaacgac ctactttcaa tcctgaaaca atggaaggat tgactggaaa agatgctatc
    22141 tggcgtaatt tcttggtaca agatagttat gaaccaggat caaccatgaa agttttcact
    22201 actgcagctg ctatcgaaga aggagaattc aacgagaatg aaaccttcca atctggaaaa
    22261 atccaagtag aagatgctac gatcaatgac catgactttg gtgaaaaagg tgtgctgacc
    22321 atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg tcatactgga acagcgatta
    22381 ggcggacgat ggtataacta tttacaaaaa ctgggatttg gacaaagtac tcattctgga
    22441 ttagatgatg aagtaaacgg tgctttacct acattaaata ttgtcgaccg agcaatgagt
    22501 gcttatggac aagcggtagg tgtgacgaat ttccaaatga tgaaagcttt cacttctatt
    22561 gcgaataacg gtacaatgat tcaacctcga tatatcagca aagtggtgga tccccaaact
    22621 ggagaagagc gaacaactca aacagaagtt ctagggcaac ctttttcaaa agaaacaaca
    22681 gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg aaaactacgg tagtgcttat
    22741 ggtgtatata gtgtacctgg atacaatgta tcggcaaaaa acggaacagc ccaaatcgct
    22801 tctgatacag ggggatatca aacaggagat acagcctacc tgtattcgat tgtggagatg
    22861 gttccttctg aagatccaga ctatgtcttg tatctaacaa tgaaacaccc taagacatat
    22921 gatcgaatgg cattggctaa aatcgcaaat ccattgatga aacgggcaat ggattttaaa
    22981 gaaactgaag aagattcaga tacagaaaca aaaacagaaa aagtatccgt agccgattat
    23041 cgtaatcttg aagcggacgt tgcagcagct gatgctcaaa aaagtggact gcaacctgta
    23101 gtgatcggta atggtaaaaa agtacaaaaa caatctactg ccaacggtga tcagttgatc
    23161 tctggagaaa aactgatcct atatactggt ggtgataagc tgatgcctga cgtaacagga
    23221 tggtcaaaag cagatatcat gaagctgggg aaaatcctcg gtattgaagt tagttttgat
    23281 ggagatggtt attgtgtcaa acaagaactt gctccatatg aaaaaatcac tgaagacaaa
    23341 cttagtttta ctttagaaga ataagaagag aaataggaga gaaaagagca tggaatggac
    23401 acaagcattg attcccattg tcagcagttg tgcaatgaca attgccgcaa tgccgctatt
    23461 tattggttat tttcaaatga aaaagcaagg tcaggcgatt cgtgaggagg gaccaaagtg
    23521 gcacaatagc aaagccggaa ctcctacaat gggcggctta gtgtttctga tcggctctat
    23581 tttgacagga atttgggttg gagcttggca aaaacagcta actcctactc ttttcatctt
    23641 gctttttgta ttagcgttat acggagtaat cggctttctt gatgatttta taaaaatatt
    23701 caagaaaaga aacatgggac tgaactcgaa gcagaaattg attggacaga ttctaggggg
    23761 aatcatcttt tatcttgttt accgttctga aggttatcca ggtgtattga atttcttcgg
    23821 actagatcta cctttaggaa tcatttatgg tattttcgct ctcttctggc ttgttggatt
    23881 ttcaaatgca gtaaatctga cagatggcat cgatggatta gttgctggct taggaagtat
    23941 ctcttttgca gcttacggtc taattgcttg gcatcagcat cagtatgatg tattaatcat
    24001 ttgcttaagt gttttaggag gtttactggg tttcttcgtc tacaacagaa aacctgccaa
    24061 gatttttatg ggagatgtcg gttcattagc gttgggcgga cttttagcgg caatttctat
    24121 aatgctaaac caagaatgga cattgctttt gattggtttg atctatgtta tggaaacagc
    24181 aagtgtgatg cttcaagtta cttcctttaa gcttacagga aaacgaattt ttaaaatgtc
    24241 tccaattcat catcactttg aaatgtgcgg atggtcggag tggaagattg atacagtttt
    24301 ttggttgatc agcattgtga cttcgttgat cacattatgg atcgtttggt aggagatgac
    24361 gggaaatgaa aaaaatatca acatatgaaa ataagaaagt gcttgtctta ggactcgcaa
    24421 aaagcggagt aagtgcggcc aaattattac atgaattggg cgcacttgtc accgttaatg
    24481 atggaaaacc ttttgacgaa aatccggaag cacaagagct gctttcttta gggataaaag
    24541 taataacagg tagtcatcca atcgaattgt tggatgaaga attttcattg atggtcaaaa
    24601 atccagggat tccctacagt catcctttag tagcaaaagc ccaagaaatg ggtattccgg
    24661 taattacgga agttgagcta gcttacgaag tagcggaatg cccaattatt gggattacag
    24721 gaacgaacgg aaaaacaacg acaacaacga tgacaggact actactgaat gcaggagcag
    24781 atcaaggaat tgcacgttta gcgggtaata ttgggtatcc agcaagtggt gtagcgcaag
    24841 aagcaaaatc agaagataaa attgtgatgg aattgtctag cttccaactc atgggaatca
    24901 cagatttccg ccctcatata gcggtgatta ccaatatcta tgaagcacat attgattatc
    24961 atggtacaag aaaagaatat gtcaaagcta aatggaacct tcaaaaaaac atgacagaaa
    25021 aagattatct gatccttaac tggaatcagt cagaactaca agaattagcg caaagaacaa
    25081 aagcacgcgt actgcctttc tcgacaaaag aagtgttgga agacggtgtg tatgctgacg
    25141 attactcaat atactataaa aaagaaaaaa taatggaaat atctgaactt ggcgtaccag
    25201 gtaagcataa tgtagaaaat gcactagctg ctatttctgt agcaaaattg tatggtatat
    25261 caaatgaagc tatacgagaa acgttgcatt ttttccatgg cgtaccacat cgtacgcaat
    25321 atgtaggaga aatccaaggc agaaaatttt ataatgattc aaaagcaacc aatattttag
    25381 cgactaaaat ggctctaagt ggatttgaaa cgtctaaggt cgtgttattg gctggcggat
    25441 tagacagagg aaatacattt gatgaattaa ttccttcatt aaaaggaatc aaagcaatgg
    25501 tcgtatttgg ccaaacaaaa gaaaagttga tggatgctgg aaaaaaagca ggcatcgaga
    25561 caatagtaac agccgattct gtagaacaag cagttccttt agcattagaa aacagtacag
    25621 atggtgatgt agttttatta tcaccagcga atgccagctg ggatcaatac ccaaattttg
    25681 aaactagagg aaacagattc atggaagctg tgaaccgatt aaaatagaaa aagtagaagc
    25741 gagcgataaa aaatgaaaat tttggttaca ggcggaggaa cagggggaca tatttatcca
    25801 gctcttgcct ttgtcaatta tgtaaagact caagaaccga atgctgaatt catgtatgtg
    25861 ggagcaaaac gaggtctaga aaacaaaatc gtccctgata caggaatgcc tttccatacc
    25921 ttggagattc aaggatttaa acgaaaaatt tccatgcata acatcaagac ggtccgattg
    25981 tttcttaaga gtatccgtca agcaaaaaaa atcctcagtg attttcaacc agatatcgtt
    26041 atcggtactg gaggatatgt ttccggagcg gtagtgtacg ctgcagccaa aatgggtatt
    26101 ccaacagtta tccatgaaca aaacagtgta ccaggtgtta ccaataaatt tttaagccga
    26161 tatgtggatc ggatcgctct ttcatttaaa gatgcggctt ccttttttcc tgaaaacaaa
    26221 gcagttcttg taggtaatcc tcgagcacaa gaagtagcag atactaaaaa atcagatatt
    26281 ttgaaaacat ttgatttgga tccagaaaag aaaacagtct tgatttttgg cggtagtcaa
    26341 ggagcactta aaatcaatca agcagtatca gtttttttaa gtgaatatat tccttcttct
    26401 taccaagttt tgtatgcttc aggagataga tattatgaag agatcctaaa taaaataaat
    26461 cctgaaatac aaaatattag tgtccagcct tatatcaaaa atatggcaga ggtcatggct
    26521 tccagtgatc tgctggtggg gcgtgctggt gcaacttcga tcgcagaatt cacagctctg
    26581 gggttaccgg cagtgttggt tcccagccca tatgttacga atgatcatca gactaaaaat
    26641 gcaatgagtc ttgttcacgc aggagcagca aaaatgattg cagataatga actaacaggt
    26701 gaatcactaa gtcagacagt caacgaaatc atgggtgatg aagaattgca aaagcaaatg
    26761 tgccgagctt ctaaagaaca aggaatccct gatgcttcaa aacgtcttta tgacttagta
    26821 aaacaaataa tcaaaaaata aaggcggtgg tcttagtgag taagaaaaaa gaaccaaaaa
    26881 aattgtctca tccgtcttct gatgaaaaca atttgactcc ttggcaaaag gctaaccgaa
    26941 aatatctggc agaacaagag atgaaaaacc aagagccaaa aaaagaagac ataacattag
    27001 atacacccct ttcagattct gaagaagaaa aaacggttca agaggaacag caagaagaaa
    27061 gccagtccga gaaaaaaatc gaattttttg aagagatcaa gcaaaagaaa aacggtggac
    27121 catataacgg ttcttttttg aaccggctgc ccaatttaaa aagtcaacgg cacaaagtac
    27181 tatttcgtcg gttagctttg atcatcactg tattgggtat accgctgatt tttttgatct
    27241 attatgtttc tccttatagc aaattacaag cagttgcagt atcaggcaat aagacagtca
    27301 acagccaaga aatcatctct gatacaaaat tgtcattagg agaaaatgtt tggggacagt
    27361 attttcatcg atctacttat atcgatcgcc ttaaaaaagc acagccaaga atcgaaacag
    27421 caaatataca tttcaaaggg atgaatgaat tcgatttgga tgtaacagaa tataaagaga
    27481 ttgctttgat cgcaaaaaac aatcaatact acccagtgat tgaaaatgga acagtattag
    27541 atgaaaaagt agcaaatcct acaaaaaatc tccctatctt ggaagagttc aaagactctg
    27601 caaaaatcaa agaattaaca aagcaataca atcagttatc atcagaatta caaaaagcaa
    27661 tttctgaaat caagtacacg ccaagagcaa gcaataaaaa tctgatccaa ttgaatatga
    27721 atgacggcaa tcaagttatt gtaaatatca ataatcttgc gaaccaaatg aaatattatt
    27781 cgcaagtagc caaagatatg gatgaaaaag gtgtgatcga catggaagtt ggtatcttct
    27841 cgcatcctta cggtagcact caagaaacgg aagaatcggg ccaatcggaa gaaacaactg
    27901 aaagtgaaga aaatgcggat caaacaaacg aatcaagtga agaggcagaa acgcaaaaag
    27961 cacaaacacc tggatcgtct agcacaaacg gatcatctga ttcaaatgat tcaagtgaaa
    28021 caagtcaaat agaagataat ccaagtacaa ctccgtcaag tttaaatgaa aattgataaa
    28081 acaactttaa aaagtaagaa tgtactttct aaaaaccagt aatttatggt agaattggaa
    28141 gcagtgaata tatctattaa taagtatatt ttaaaacgga cgctagttgt tcgcttgtat
    28201 atggaatagt aagtaggagg gaatcccatt catggcaaaa acaggaatgt atgtaggcct
    28261 tgatattgga acgacatctg ttaaagttgt cgtggctgaa tatatcgaca gccaaatgaa
    28321 tattattgga gtaggaaacg caaaatcaga agggattaac cgaggcatta tcgttgatat
    28381 cgataaaacg gtccaagcga tacaacgagc agtacgacaa gcagaagaaa aagcaggtat
    28441 ccagatcaaa ggcgtaagtg tcggtttgcc agcaaacatg cttgaagtag aaaattgcca
    28501 gggaatgatt gcagttaacg gagattcaaa ggaaatcacc gatgaagatg tcagaaatgt
    28561 ggcttcagct gcccttgttc gttcaatacc tcctgaacgt caaatcgtgt caatattgcc
    28621 acaagatttt actgtcgatg ggttcgaggg aatcaaagat cctcgtggaa tgatcggcgt
    28681 aagattagaa atgtacggtc tgttgtttac aggacctaaa acgatcatcc ataacatacg
    28741 taaatgcgtg gaaaatgccg gattaatcgt caacgaaatg gtgattactc ctttagcgct
    28801 gactgaatca attctttctg acggagaaaa agattttggt acgatcgtca ttgatatggg
    28861 tggcggccaa acaacaacgg ctgttatgca tgataagcaa ttgaagttta ccaatcttga
    28921 ccaagaaggt ggcgaatttg tcactaaaga tatctctatt gtattaaata cgtcatttaa
    28981 caatgcagaa gcgttgaaaa taaactacgg ggatgcatac ccagaacgga catcaccaga
    29041 tgaagaattt ccagtagatg tgattggtca gtcagaacca gtgaaagtcg atgaacgcta
    29101 cttgtcagaa attatttcag ctcgaatgga acagattttc aacaaagcaa aagatgcatt
    29161 ggaccaaatc gaagcattag agttgccagg tggcgtcgta ttgacaggcg gagctgctag
    29221 tcttcctggt gttgtggact tggcacaaga aatcttcggc gtaaatgtaa aattgtatgt
    29281 tccaaatcac atggggctga gaaacccggt atttacaaat gtaataagta tcgtggatta
    29341 ttcagcaaac ttgagtgaag tgtatcaatt ggcaaaaatt gctgttacgg gtgaaacctc
    29401 agcagctcgt caaatggtag ttgaacaaga aacaacaaac acatatgaga gctatgaagc
    29461 acctgaagaa acaatctacg atgagccgga accaaaagag tccggagaaa atgtaaaaaa
    29521 taaaatcaag ggtttcttta caaatatttt tgattaattc atcctgggag gaataagata
    29581 tggaattctc aattgataat aatatcaatg acggcgcagt aatcaaagtc atcggagtcg
    29641 gtggtggcgg tggaaatgct gtaaaccgaa tgattgaaga aaacgttaaa ggtgttgaat
    29701 tcattacagc caacacagac gtgcaagcat taaaaaattc aaaagctgaa acagttatcc
    29761 aattaggccc caaatatact cgtggtttag gtgctggttc tcagccagaa gttggacaaa
    29821 aagcggcaga agaaagtgaa caatccttac gcgaagcttt agacggtgca gatatgattt
    29881 tcatcactgc aggaatgggt ggaggaaccg gtacaggtgc tgcaccaatc gttgcaggta
    29941 tcgcgaaaga attgggcgca ttgactgttg gtgtcgtgac acgtccattc acttttgaag
    30001 gaccgaaacg tggccgtttt gctgcagaag gtatcgctcg tttgaaagaa aatgtggata
    30061 cattattaat tatctcaaat aaccgtttat tagaagttgt agataaaaag acaccgatgc
    30121 ttgaagcttt ccgtgaagca gataatgttt tacgacaagg tgtacaaggt atctctgatt
    30181 tgatcacagc gccaggttac gttaaccttg actttgctga cgtgaaaaca gtgatggaaa
    30241 accaaggtac agcattgatg gggatcggtg tggctagtgg agaagatcgc gtagtagaag
    30301 caacgaaaaa agcaatctcc tctccattgc ttgaaacatc tattgatgga gctgaacaag
    30361 tacttcttaa catcacaggc ggtttggata tgacattgtt cgaagcacaa gatgcttcag
    30421 atatcgtagc aaatgcagca actggcgatg taaatatcat tctaggtaca tctatcaatg
    30481 aagaaatggg cgatgaaatc cgtgtgacgg tcatcgcaac aggtatcgat gaatcaaaaa
    30541 aggaacgcaa gtctagccga ccagctagac aagcacaaat gcaatcacct gctcaaaaga
    30601 ctgtattaga tatggatcaa gctaaaccaa cttcttcaga agaagaaaac agttttggcg
    30661 attgggatat cagacgtgaa caaaatgttc gtcctagagt tgatgattcc aattttgaca
    30721 atatcgaaaa aaaagaattt gatacattca accgcgaaga aacaaaatct catggcgatg
    30781 atgaattaaa tacgccaccg tttttccgtc gtaaaaaata aaggagagga agagcacatg
    30841 attgctgaca acttgcagaa aatcaagcaa gaaattcagg attcgtgtgc tcttgttcat
    30901 cgtgatccga aagaagttac attagtagct gtaacaaaat ctgttgaatc cgctcaagct
    30961 gaagaattgg ttagagctgg cgttatgaat tgtgcagaaa accgagtaga taaattatta
    31021 gaaaagaaac aagatttatt tgattttcct gaaataaagt ggcatttaat tggtaattta
    31081 cagcgcagaa aggtaaaatt gattgtaaat gaaattgatt actttcatgc gttggacagt
    31141 ttaagattag cagaagaaat ccagaagcgg ttagagaaag aattatcctg ttttattgaa
    31201 gtaaatgttt ctggtgagga atcaaaacat ggaatttcac cagatgagct tttaccgttt
    31261 gtggaatctt tagctgcctg cacaaaaatc aaggttgttg ggttgatgac tatggcgcct
    31321 aaagaagctt ctgatgagca aatccataaa gtgtttggta cgctgaaaga attgcgtgat
    31381 cttgttcaag caaaaggtta tgcccatgca ccatgtagtg aattaagtat ggggatgagt
    31441 caagactttc aaattgcgat tgaagaaggt gcaacattta tccgtgtagg aacagcgcta
    31501 ttcaaagacg aataaaggag gaacaaaaat gccgctttta aacagaggag caattgctaa
    31561 ttttttcgga ttagctgatg aggaagagta cgaataccaa gattatcctg aaacaacaaa
    31621 aaaggctgta cagccaacta aaaaaaatac aacacagaat caatcaactc attctgtatc
    31681 tgctgcgaat actcatgaaa aacaaaaaac ggcgacttca cgtcctgtaa cgcatgaaag
    31741 tattcagccg gctgaaaaga aaaaagtaat tgaactacat caatcaccta caaaaactgt
    31801 agcgaaagaa agaacagctt caagaacctc tacacaacct aaagcaaatc ttgctggaaa
    31861 aatcactgta atggaaccaa gagcttattc tgaagctatg gcgattgcta aacgaatcat
    31921 tgctggagaa gcaaccttag tgaattttca tttagtagaa gaaaaacaag cgagaagaat
    31981 cgttgatttt ctaacaggaa cagtttatgc actagatgga gatatcaaac gtgttggaga
    32041 tgagattttt ctttgtacac cgactgggat ggagattgac agttcaaccg ctcaatcttt
    32101 tgcagatgcg aatttgtttg atctgtaaga ggaggaacta cgatttatac tattgcacta
    32161 ttacttcatt tacttcaacg ggcagcctat ttctatagca ttatcttgat ggtatatgca
    32221 ttgctctctt ggtttccagg cgggtatcaa tcagggctag gacgattatt agctaagatc
    32281 tgcgaaccgt atatcagttt gttcgatcgt ctgcctttga ggattggacc cttggatttc
    32341 acaattttag gggcaatcct gatcttgaac ttagccgtac aaggtttagc acagatcgtt
    32401 atatggataa ctgtccattt agtttattga ttgaagcaac aaaatcttta ttctcagaat
    32461 agataggagg ggaaaatttt ggatgcaaat gtataccagc actttcgggc agatgaacgt
    32521 acatttatcg attcggtagg cgattggatt gaacaggttc aaggacaata tgcaccctat
    32581 ctgaccgaat ttcttgaccc aagacaatcc tatattttag aaacactgat tcgacaaagc
    32641 agtgatttgc gtttcatgtt ttatggcgga tatgaacagg cagaaagaaa aagatgtttg
    32701 attttccccg attattacga acctgtagag gaagattttg aaacagcatt gtatgaaatc
    32761 cattatccta gcaaatttgc cagcctaagt catgggaaaa tccttggaac tttagtggga
    32821 acggggatca aacgctctta ctttggcgat atcatctcgg acggtgatcg ctggcaagta
    32881 tttattgcaa atgaggtgga tcattttgtt gctgcacaag taacaaagat tggaaaggtt
    32941 gctgtacgct tggaaccaat caaatacaca gaaatgatta tgccgaaaga tagctggaca
    33001 caagaaagag gaacagttag ttccatgcga ctagacagtg tgatctctga gatttataat
    33061 atttccagac agcggtccaa gcagctgatc gaagctggta aagtaaaagt gaactggaca
    33121 gaaaatacaa gacctgattt tgtacttgaa ctattagata ttatttcagt cagaggttat
    33181 ggcagagtcc agatccaaga acttgagggg aagacgaaaa aagaaaagta cagggtactg
    33241 ttcggtgtat tacgtaaata aaataaaaag gtggtttact catggcttta actccattag
    33301 atatccaaaa taagacattc ccgacaaaaa tgcggggata taatcaagac gaagtagatg
    33361 atttcttaga tttagttgtt cgtgactatg aggaattgac tcaaagaaat cgagaattag
    33421 aaaaagcagt caaacattct gaagaaaaac ttgagtactt caatgaatta aaagatgctt
    33481 tgaaccaatc aatcatcgta gctcaagata cggctgataa agtaaaaaca agtgcaagca
    33541 aggaatccga agtgatcgtt acttctgcac aaaacaaagc agatgagtta gttgcgaacg
    33601 ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga aaaagcaaga aaaatcttga
    33661 cagatgcaac agaaaaagca agacagcttg cgacagaaac tgaagatctt aagaaaaaaa
    33721 cacgagtatt ccatcaacgt atcagcttga tgcttgagtc tcaattagaa caagtgaaaa
    33781 gtgcggaatg ggatgaaatt ttacaaccat tctccagcta cgtgactgac agccatgaag
    33841 tgatcaaaga agttttagca aaacagcttg acaatgaaaa tgatactgat gtaaactcag
    33901 acacaggagt catagaagca cctatgacgg catttgatac acaggctatt gatatctcga
    33961 tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc actttgttat tcttaaagcg
    34021 agccgatgag agtggaagtt cggtaagaca ctgcatggtg aaagatcctc tacttgagac
    34081 ctgcttttcc gaaattagag taagaagcag cggctgacct cgttattggt cacagagagt
    34141 ttgcactaga tagatgcaac taaatttggg tggtaccacg gcatgttcgt cccttgcggc
    34201 tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt gtatggttga atgaaaatga
    34261 aagaaacctt gcaattagga aaaacaaaat tccctatgcg tggtaatttg ccaaaccgag
    34321 aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta ccaaaaaaga caagagttga
    34381 acgaaggaaa acctagcttt gttcttcatg atggacctcc atatgccaat ggtaatatcc
    34441 atttaggaca ttcattgaat aaaatcagta aagacattat cattcgttca aaatcaatgt
    34501 ctggctatcg tgcaccatat gttccaggat gggatacaca tggattgccg attgaacaag
    34561 tattggctaa taaaggcgtg aaacgtaaag aaatgtccat ggcagaatac ttggaaaaat
    34621 gtcgtgaata tgcactttcc caagtagata agcaacgagc agatttcaaa cgcctaggag
    34681 ttgcaggaga ttgggaacat ccttatgtta cattagatcc ttcttatgaa gctgcacaag
    34741 tacgcgtatt tggtaaaatg gctgaaaaag gctatattta caaaggattg aaaccaattt
    34801 actggtctcc gtcaagtgag tcttctttag cggaagctga aattgaatat aaagatgtaa
    34861 aatctccatc tatttttgta gctttcaatg tcaaagacgg caaagatatt cttgatgaag
    34921 atacagcttt tgtcatttgg acgacaacac catggacatt gccagcaaac caaggtattg
    34981 ctgtaaatcc aaaatttacg tatgtattgg tggaagccga tggccgtaag tttgtcgtag
    35041 cgaaagattt gctagaaaca gtccaatcag aaatcggttg ggaaaatgta acagttctaa
    35101 aagaatttgc tggacaagaa atggaatata tgactgcaag tcatccattc tatgaacgtg
    35161 aatcacttgt gattttaggc gatcatgtta ctttagacgc tggtactgga ttagtccata
    35221 ctgctccagg acatggggaa gatgactata tcgctggtag caaatataaa ctatctgtag
    35281 tgtcacctgt agactcaaaa ggattgttta cagaagaagc acctggattt gaaggtatct
    35341 tttatgacaa agcaaatcct atgatcactg atctattaaa agaaaaagga gctttgctga
    35401 agctagattt ctttactcat agttatcctc acgattggcg gacgaaaaag cctgtgatct
    35461 atcgggcaac tccacaatgg tttgcatcta tcgataaatt ccgtcagaat attttggatg
    35521 aagtagaaaa agtagagtgg gttatcccat ggggtaaaac acgtttgtat aatatgatcc
    35581 gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg gggagtacca cttccgattt
    35641 tctatgctga aaatggagaa gcaatcatca caccagaaac aatcgaacat gtggcaaaat
    35701 tatttgctga acatggatca attatctggt ttgaaaaaga agcgaaagaa cttcttcctg
    35761 aaggatttac ccacccaggt tctcctaatg gcgaatttac aaaagaaaaa gatatcatgg
    35821 atgtctggtt tgattctggt tcttctcatg aagctgtttt acgtcagcga ccagaattat
    35881 cattcccagc agatatgtat ttagaaggat cagaccaata tcgtggctgg ttcaactcaa
    35941 gtatcacgac tagtgtggct atcaatggtg tagcaccata caaatcagtt atttctcaag
    36001 gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc tttaggaaac atcattgcac
    36061 cagaaaaagt gatcaaacaa tttggtgcag atatccttcg cttatgggta tctagtgtag
    36121 attatgaagc agatgttcgt gtttctatgg atatcttaag tcaagtggca gaagtatatc
    36181 gtaaaatcag aaatacgatg cgtttcttat tagcgaatac agaagatttc gatcctaaaa
    36241 aagatactgt ttcgtacaat gatttacgtt ctgtagacaa atatatgacc gttcgattga
    36301 accaagtaat caaagaaatc cgagaagaag ggtacgacaa atataacttc atgcatattt
    36361 accgtacagt tatgaacttc ttgacggttg atctatcgtc attctacctg gattttgcta
    36421 aagatgtagt ttatattgaa gcagaagatg attatcaacg tcgttgcatg caaactgttt
    36481 tctatcaaac agctgtagct ttgacaaaat tattgactcc tatcattccg catacagcag
    36541 aagaaatctg gtcattcctg aaagaagaag aggagtacgt tcagttaagc gagtttcctg
    36601 gttatgaaga gtttgcaaac caagaagaat tgatggatac ctggactgct ttcatggact
    36661 tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa cagcaaattg attggtaaat
    36721 ctttggaagc aaaactaact gtttatccaa aacaacaagt tcgtgaaatg ttaaaagctt
    36781 tggatgcaga tattgcacaa ttattgatcg tttcaccaga ctactttgaa gttatggcag
    36841 cagatgaaca agtgccagat aacgctatgg tatttgatga tgttgctatt acagtggaaa
    36901 aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg taaagatgta ggagtagatg
    36961 aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt tgaagacttc tatcctgaag
    37021 ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg gggtgtgaca tgttctttgt
    37081 cacaccccct tttgttatca atcgtttaat tttccacgtt cacgatacca gttatctggt
    37141 tgccagatcc attcgaatcc gtctttttca agtagttcga aagctgcttt aggtcccatt
    37201 gatccagctt tgtagttggg aaaatccggt tgtgtttggt cccaagcttt tcgaatgaca
    37261 tccacgatat gccaagattg agcgacttct tcccaatgag agaagttcgt tccatcacca
    37321 ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc tattttcaac catttctgca
    37381 ctatttctaa attccaattt gattggttcc gtttcaaatc cttgccctac ttctttccca
    37441 ttcaaggata aagaaaatcc ttcagttggc tgaatataga tcgttagaac attaggtgga
    37501 agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga atacattaac aggaacttgt
    37561 ttgaatacga tgttgatccg agtccctttt tcagtcaaac gtttgcctgt acgtacatag
    37621 aaaggaacac ctgaccaacg gaagttatcg atagtgaatt tgcctgcgac gaatgtttca
    37681 gtctctgatt gagcatccac tttgtcttct tcgcgatagc ccttgaattg tacaccatcc
    37741 aattcacctt gagcatattg cccgcgaacg aagttttcta acacttcttc ttcgttataa
    37801 atgcggatag ctttcaatgc tttgatcttt tctgtacgga tttctttttc ggaaaatgct
    37861 actggcggtt ccattgcaag taatgctaca acctgtaaaa tatgattttg caccatgtct
    37921 ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt ctacaccgag gttttcgcca
    37981 aaagtaattt ggatattgtc gatatagcga ttgttccatt gagcctcaaa aatattattg
    38041 gcaaaacgga tagctgagat gttttggatc atttcttttc ctaaataatg atcaatcctg
    38101 aaaatatctt gttctgggaa tacttctctg atttgctggt tcaattcaaa agcagactgg
    38161 taatcagaac cgaacggttt ttctatgatc aaacgattga agccattttt tgtcataata
    38221 ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt gaggtgccat agccaaataa
    38281 tagacacggt taccgtttaa ttgatagtgt ccatctaatc ggtcagacaa ttcttttaat
    38341 gtctgataat gcgttgaatc atttacatca tgggattgat agtaaaaatg gcttgcaaag
    38401 gtagttgctt cttcatctga aggattcaat gcacggattg tttctttgac aacttcacgg
    38461 tagtgctcat cgctccaggg acggcgagcg gttccgatta cagcaaaatg ctcgcctaaa
    38521 tttccttttc tgtaaaggcg gaacaaagac gggtacaatt tacgctgggc taggtcacct
    38581 gtaccgccga atatcgtaaa caatacattt ttttcattag acatgtggat acactccttt
    38641 atattttatc gacaaaaatc gtgctggccg ccttaaagct gacggggatt ttctttctat
    38701 cattttggat agtgatcggt ccttcataag ctgcgatgtc aacgatctca tattcttcgt
    38761 tgatattcag gtcaatagtg actaagtaat ccaacaattc tttttcatca agcactcgtg
    38821 cgatacgcac ttttgtaccg acaggatagg actccaatgt ttgtcggcgt tcttcatgga
    38881 caacttcctc ttttttagga atcgatcctc catgaggaca atgttcagga tgattcaaat
    38941 agttttctaa atgatctgca agatgttccg aagtcacatg ttccaatact tcagcatcat
    39001 catggacttc attccagcta tagtctaaat gttcaactaa aaaaacttcc cataagcgat
    39061 gtttgcgaat cagactgcta gctttttgca gtccgcttgc agtcaattgt acaccttgat
    39121 aaggggaatg ttctacaagc tcttctttca caagtttaga aatcatttca ctgacagatg
    39181 cagctgagac atctagacta gatacgattt gtttattgtt gactttcgtt gtatctccac
    39241 ctagttctaa aatgattttc aaataatctt ctcgatttgg tgtcatattc gatcatcctt
    39301 tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa acgttaattg actatttttc
    39361 taaataaaaa acaaaggatt tttagacgtt tttacaacaa aaaacgaaag atgatagaga
    39421 agactcattc tttcgtttat aaaggatata tatatacatt gggagggatc tatttttcag
    39481 ataacaactt atatgaagtg tctgcagatt cgtcataaag agtttgttcg ggataagaaa
    39541 agataagata aaaaccatac tgatcacgta cgactaaacg atattcatct atttctacca
    39601 cagatgtgcc aagagactga ccgttgatca aaaacgtgta atcagatgta atctgtaatt
    39661 gttgtttttg ctggctatca ctaaagaacc atgtaccttg atactttgag agtccttctg
    39721 acttttccgc ttgttttttc tttttcaagt aaataaaact tgagagaacg gtaatgagtg
    39781 aaccaagaaa atagatcatt ttttttatca ccaaatcact cctttatgag aaaataatcg
    39841 catagacata ttctttcttc tttatttcat tgtaccgaat aaaagctaaa atttctctaa
    39901 gactttcgac tggttttttt actgtttttc tacaaattat gataggatac atagtaggaa
    39961 gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag ctttttttat tgaccgcccg
    40021 aatcggttta tagcacattg tatgaaaaaa gacggagaaa taattattgc gcatgtcaaa
    40081 aatacaggta gaggaaaaga ggtcctgata ccaggcgctg aagtggctat tgtatttgct
    40141 cctggaccta aacggaaaac cgcctatgat ctgattgctg taaaaaagaa gggtgactgg
    40201 tttaatatcg atagtcaatt gcctaatcgg ctggcaattg atggtatttt agacggcacg
    40261 atccttttac caggattaac tggtccattt acggtataca agcgtgaagt aacttatctt
    40321 cactccaaat ttgatattta tttagagacg gtggtcggac aaaaagcatt tgttgaagtc
    40381 aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc ctgatgcgcc cacactcaga
    40441 ggattgaaac atataggaga attaacgtat gccgcacaag atggttatgc cgcttatgtc
    40501 ttgtttattg ctcagttcga acatcttcat ctggcaacga tccatgaaga aatgcaacca
    40561 gcactagctg atatggttcg acatgcgcag caaagcggag ttcaaatcct cgcctataat
    40621 tgtcaagtat cggcagatga ggtaactgtc aagcaggcca ttccatttga tgtagatatc
    40681 ccttttgaag atcctataca atagaaagaa ggtaaaataa tgatcaactt aggtattatc
    40741 ggaacaaatt ggattactca tcaatttgtc caagcagctt taagcacaaa aaaatatgaa
    40801 ttgacagcgg tatattctag acatttggaa aaagcacagg aatttggttc tcattatgaa
    40861 ggggatatcg aattcgcaac agatttgaag acttttttca atattactca tatggacacc
    40921 atttatattg cttctccgaa tagtcttcac tttagtcaag ctaagcaagc aattttggca
    40981 ggaaaaaatg tgatagttga aaagccagct ttttcgacgc caaaagaaat ggatgaaatc
    41041 atccatttgg caaatgaaca caatgtgtat ttttttgaag cagcaagaaa tatccatgaa
    41101 tcaggtttta aaaagattgc cgattactta ccattaaaag acgagataat cggtgcaaat
    41161 ttcagctata tgaaatattc ttcgcgctat gaccaagtat tagatggaga agaaccgaat
    41221 atcttctcga ctcatttctc aggtggtgca ttgatggatt taggtgttta ccttgtatat
    41281 gcagctgttg cttggtttgg aatgccaaaa gaagtccatt atttcccaag aaagatttct
    41341 actggtgtag atggtcttgg atgggggatc ttacgttatg agaattttga tgtatcgatc
    41401 cagccaggta aaatcggtga ttcttattta ccgtcagaga tttattttga ctcaggaacg
    41461 ctagtgatga atggagtcaa tgcaattgaa caagcacaat ttcatgatcg cgcgcataaa
    41521 gaaatcattg atttggcggt aacagctgca gaaaatccaa tggcagatga agcagaggat
    41581 tttgccaatg tcatggctca tccgaaagat ccagcttggg gattgttgta tgaagagtgg
    41641 atcgagctag ctagaaatgt gaaccaagtg atttatgatt taagaaaaaa tggcggcatc
    41701 aaattcgatg cagataatga ggaagatttt taatgaacga atttgtacaa gtatttcccg
    41761 aaaattggca gaaaaaatat gaggataaag ggtttatctc accttctctt attcaacgag
    41821 cagtttttca gccactaagt aataaacaaa gtatagtagc ggtttctcct acaggttcag
    41881 gtaaaacact ggcttatctt tggcctctac tactaaacgt ggaaccaggt gaagcaagtg
    41941 cgttggtcat ttttgcttct tctcaagaac tagctattca agtagcagat gttgcacgtg
    42001 aatggggaaa agacaaagag ttgaaagtcc aatctttggt gggcggagcc aacgtcaaac
    42061 gtcagattga gggtttgaag gaaaaaccag aaatcttgat tggtacacca ggaagaatat
    42121 tggaattgat gaaagcaaaa aaaataaaag cacatcaagt gaaaacaatg gtatttgatg
    42181 aagcagacca gctatttgac gctgggaaca gccaaatcat tgatcaaatt cttcatcaag
    42241 ctccaacaga gtatcagcta gctttttttt cagcgacagc agatcgttcc cttgaaagca
    42301 ttgaaaaaat cactgggaaa acgtttgaga caattgacgt gacagcagag gacgattctc
    42361 gaaaaggact gagacattac tttttacgtg taccgatacg aaagaaagat gactacttga
    42421 gacgtctcac gcatatagaa gattttcatg gtctgatttt ctttaatcaa ctgactgaat
    42481 taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc ggtaggaagt ctggcctctg
    42541 atcagaacaa gctactccga aaagcagcgc tagaacaatt caaaaagaaa atggtcagag
    42601 cacttttgac cacagatgtg gcggctagag gtttagatat tacaggtctt ccttatgtag
    42661 tgaatgcaga agttccgtta agtgaagaag cttatttgca tcgttcggga agaactggtc
    42721 gaatgggtaa tgaaggaata gtaattacac tcgtacaaga taataatcta agagatttaa
    42781 aacggctttt acgtccatca aatataacac ttgaagagat ttttttatac ggaggtaagc
    42841 tgcaaactga gcagccaacc aaagaagatg cagctcctgc agtaaataaa aagtatgcga
    42901 aaactttttc tggaaaggag cagaacccag aatataacaa agaaaaaata agaaaaaaag
    42961 ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag gcgcaaataa gcgccttttt
    43021 atgctatgat tatgaaaacg ggaacaaaat tggttgccag aggaggggtt atgtgaaaga
    43081 acgtcaagaa gagatcttgc agctattgac agaaaaccaa caaggattga cagcaagtga
    43141 tgtagctgag cgcttaacta tcgatcgaag caatgcaagc cgatatttaa gtgaactata
    43201 caaggcccat catattgtaa aaaccgctgg ccgaccagtc gtttactctt tgccaacgga
    43261 aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg caagtgactt ttgaaacact
    43321 cgttggagaa aatgattctt taaaagtcag catacagcag gcaaaagctg cgattcttta
    43381 tccaccgcgg gggttgcata cgattatttt tggagaaact ggtacaggga agtcgatgtt
    43441 tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg ctttctgcag atgcgccatt
    43501 tgtttctttc aattgtgcgg attatgctca aaatccgcaa ctgctctttg gacacatctt
    43561 tgggatcaaa aaaggggctt acacaggagc agctcaagat agtcccggtc taatagccaa
    43621 agcagacggc ggtatattgt ttttagatga gatccaccgt ttaccgccgg aagggcagga
    43681 aatgctgttt actttcattg ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt
    43741 acatgaagca tctgttcaga tcattggggc gacgactgaa tcttcagaaa gtttcttatc
    43801 tacgttcaat cgacggattc ctatggcaat caccttgccg aatcttgctt cacgttcact
    43861 ggatgagcgt tatgaaatca tctctctttt tatcaaacaa gaagcgaatc gtttgaatca
    43921 acgtatcgat atcgaaaaag aagcgattct tgccttcatg ctttatgatg cggaaggaaa
    43981 tatcggtcaa gtgaaacgag acttgaaact agtctgtgca aaatcttttt tacattaccg
    44041 tactcatcat gagaaaaagc taatcattcg aaaagaagaa ctttcccttc aagtacaaaa
    44101 aggattactg aaaatcaagg aaataccgga acgtctcgac cgattcatgg acagcaaaag
    44161 tcagtatttg acatttgaac caggatttgc tgatgttgtc tggtcacaag atccagaacg
    44221 aaacatgcag gtctataacg atattgagga aaaagtcact tctctttccg aaaccggtgt
    44281 ggagaacatt gatctagaga ccctcatttc aaaagatgtg gatgcttatt tccagacata
    44341 tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt ttgccagatg aaatctggcg
    44401 gttaaccaat caattgtatg atgtggctga aaaacggtta gaccgtattt ataatgaaaa
    44461 agctcgtttt gcttttgctt tgcatctgca aagtacattg gatcgtgtga gagaaggtca
    44521 tatgatcgtc cacccagatt tgaataacgt acggaaaaat tttaagaggg aattccaggt
    44581 agcaatcgat ctttcaacga tcattgaaga agaagaacaa gttgagattc cttttgatga
    44641 aatcggcttt attagtatgt ttctttctat caatctagga gaaagtgagc ctgttaaaga
    44701 aaacaaagta gatgttctca tattgatgca cggcagacag actgcttcaa gcatgttgga
    44761 gatggcacaa gaactattag gaacaaaagt agggaaagct tttaatatgc cactggttat
    44821 ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc aaaaactatc aagagcattt
    44881 gacgaatggc ctactcttgt taacagatat gggatcatta aacaccttca gtgatttgat
    44941 ttatgaagag actggcattc ggacgaaagc tatcacgatg acaagtacca tgattgtgat
    45001 agaagcgata cggatggcaa gtgttggaag aagcttagaa gatatttatc aaaatatcca
    45061 gctttccttt gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg aaactttaca
    45121 gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa ggagtagctg ccaaattata
    45181 tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt
    45241 agaacgtgaa tcattcaaaa aacacatcga tgccttattg gaagaatatg agatcaaagc
    45301 aattgcggga acagttgatg ttgattatca aaatatccca tttttttcag cctatgacgt
    45361 ttttgatgat gaaaaactaa atattttaaa gcgtatagct gatgatgaga tcccgacagc
    45421 taaaattgtc gattcacttg ctggatcatt aagcgatgtc ttttctattg atgaactgat
    45481 ccgttcattg caaaagacag ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc
    45541 tggagtagat actggaatcg ttatccattt agccttcatg ttagacggat tgatcaaaca
    45601 agaaagccat cggcaatttg agaaactgtc tattttctca aaaatttatc gtttggaaat
    45661 ggatatcgtt cgggcaaact tgatggcact agaacgaaaa tatcaggtaa aaattccaga
    45721 agatgatatc gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca
    45781 ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag
    45841 catgaaaacg ctatttcagc tctttttttt attggcatgc ttcttgcgtt atataaaagt
    45901 gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag
    45961 cagggatgag tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga
    46021 tggaagccga tatttttgcg gtctctgcct cagatgcaga taataattta gaatctaaga
    46081 atgtcgatgt attattatta ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa
    46141 aacttgcgcc taaaggtatt ccattagatg tcatcaatat gcaagactat ggcatgatga
    46201 acggagaaaa agttcttgca caagcagaaa atctaatgaa ataaatcaag tatgcagttg
    46261 ggggtataac aacgtggaag atcaacaaaa tctagaagcc atcatggggt taatcatgtt
    46321 tggcggtaat gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa tcggtgattt
    46381 tgagttagcg gataggaaaa tcgctgatgc agaagaatca cttatacaag cgcatcatgc
    46441 acaaacagac atgttgacac aagaagcaca aggaaatcat atgcaagtga cattattgac
    46501 tgttcacagt caagatcact taatgacatc tattgcgttt actgatttag caaaagaaat
    46561 catcgaactt tatcgtcgcg tatacagcga ataaatacaa cttctttttt gaacggaaga
    46621 agaaaaattt ttgaacggat tttttatttt gttataagga gaactctctt tatataaaaa
    46681 acttatagga ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa atacatcctg
    46741 ccggttgccg caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga tgcttttatc
    46801 ggcacactgc ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt
    46861 gatttaccgc aacagtttaa cagtacctat gctgacagtt tagcagcaga caaaggaatc
    46921 tttgccgtat tgaatgtcat catcggaatc aatggttttg tctggaacgg tacactagca
    46981 atcgccggat tgatctttgc cttttcttgg gggtataatc ttgcccgagc ttatggagta
    47041 aatgatctag ctggggggat cgttggttta gctaccttga ttcaaggtat ttcctttggt
    47101 ccagcattat cagcagcatt agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat
    47161 agttctgaga ttggtgcaac gattgcagat ggcacactgt cagttggatt atggggttgg
    47221 ctaaaactag atcatttgaa tggaaacgct tattttacag ttatgatcat gggagctatt
    47281 tcagtcatta ttttttgtaa attgatgctt gccaacatta cgatcaaaat gcctgattca
    47341 gtaccaccag cagtatctaa agcatttgca gccattttac cagcgacgat cgctttatac
    47401 gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg atgggcaact gatcattgat
    47461 ttggtgcaaa aatatattgc agaaccattc ctaggattat ctcaagggtt gggagcagta
    47521 ttgatcgtca cagtatttgt tcaagtattc tggttctttg gtattcatgg accaaatgta
    47581 ttggcaccag tgctagaggg aatctgggga caagcacaac taatcaatat tgatattttc
    47641 caaaaaggtt ataacggaaa aactggtaca ccagccgtgt tagatgcaat cgaagatgga
    47701 aaagcttata tgtgggtccg cggatcgttc gatgcttttg catggtttgg cggatcaggc
    47761 ggaacgatcg tcttactgat agctatcttg attttctcta aacgtgcaga ttatctaaca
    47821 gtagctaaat tatccattgg gcctggtatc ttcaatatca acgaaccagt gatgtttggt
    47881 ctgccaatcg tattgaatgc aatcatgttt atcccattct tgattgctcc agtagtagca
    47941 acagcaattg gatggacagc tacttatctt ggcctagtcg ctccggtatc gcagcaagtc
    48001 acatgggtag taccgccgtt cttattgtca ttcttagcaa caggagcgga ttggcgggca
    48061 ccgatcgtaa cattagtatg tatggtcgtg actttcttga tttgggcacc atttgtcatc
    48121 tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg
    48181 gacgctttaa gtttcgttcc tcataatgaa ttttctagga ggaactgaaa tgattacaat
    48241 tgccagtgca atgcaacaag atgaaatcaa agaattgtta gaaggataca atgaagaagg
    48301 aatgactttt acatttaaag aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga
    48361 agatccggaa gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc cttggggaac
    48421 agtcctttat tttcaatcaa cagctgttta gaaaatggtt taaaaagata ggatggttga
    48481 gatgataaaa aacggtttat ttttatgtag tattggttct ttgatcgtgt tgtatagttt
    48541 gaatccacat tcgcaaaggt atgatttgta tagtatggtc acaggtcttt ttctaacagg
    48601 attaggaatc tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa
    48661 ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt ttttttacga taaagttatg
    48721 gactctgtcc ttttcgatgt acgtaaagcg accggcaaat cagtaactag aaaacagctg
    48781 aaaaatttgg agtacgtcaa acaattctcg caaaataacc gggctcgcat aaaaatcgaa
    48841 gaagtcattg aaaaccagtc ttataagttt cgtacatcga caacgaagaa tgaattcgtc
    48901 gttcattatc aaatcaaacc attggacgat tcgacctgtg aggttcatta cacagaaaaa
    48961 atggagtctt atggcatgct tcaaaagttg aatgatatgc ttttaggaac aatcttgatg
    49021 tattttaaac ggagacgttt caaaaaaatg ctagatatga tcgaagcaac gtattaaatc
    49081 aatgaaacat ggtactattt agttattgaa aaatgaagta agctagaagg aatttttcat
    49141 aggaagatag atctttcctg cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa
    49201 tgacaaacaa agtaagagag ctaggtgtat cttcgattga ggaaatgttt gatttagcca
    49261 cttatgcatt taatggggca gatacacctg aaaggcgaga acgttttcgt acacttgcgg
    49321 agaattcttg gaattacgga ttttttgatg aggaggatcg gctaacaagt caagtcatgg
    49381 cgaccccttt tccagtgaat ttttacggac aagagtacct aatggccggc attggctatg
    49441 ttgcttctta tccagaagca aggggacaag gcgggatcaa tcagatcatg gaaaagatcc
    49501 ttgaagattg ccgtgatcgt aaagtaagct tatcttatct ggcgccattc tcctatccct
    49561 tctatcggcg ctatggctat gaacaatcat ttgataaaat cagttatcaa ctaaacagtc
    49621 gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg
    49681 caaaagctgc aatcagaaga atttatgaac agatgccgga aaaccaacga gctggtctga
    49741 agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa aggcaattac cagtttgctt
    49801 tgcattttga tgataatgag gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt
    49861 cctttattat tgcggagtgg ggatttttgg atcatgatgc tttttgcagc cttgttcgct
    49921 tcgtaagttc tcacaatggt gcttttgaaa catttgaata ttcatgcggc tacggcggta
    49981 atgatcaaaa ttatctatta gaaaatccat ttgcttctgt aatgatcaca ccttatatga
    50041 tggcgaggat cgtagatatt tctttgtttt tggaaaatta tccatttaaa aagagacaag
    50101 ctgcatttgc cctacaaata gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg
    50161 aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa
    50221 tcgttcgagg aagtatccaa catatcacgc agctattatt agggtatcgt aaagtggaag
    50281 agttggtatt ccaagaaaga atacaggtga caaaagaata tgaagaaacg ttaagctcaa
    50341 tcttgccaca gcaacagcca attcttaatg actatttttg ataaaaaacg ggtgatgaca
    50401 atattttt
//
LOCUS       DAGCXG010000009        49991 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGCXG010000009 DAGCXG010000000
VERSION     DAGCXG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.009"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            149..1369
                     /locus_tag="MB022_000536"
     CDS             149..1369
                     /locus_tag="MB022_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBT4465563.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1366..4086
                     /locus_tag="MB022_000537"
     CDS             1366..4086
                     /locus_tag="MB022_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4465564.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4070..5014
                     /locus_tag="MB022_000538"
     CDS             4070..5014
                     /locus_tag="MB022_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4465565.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5083..6093)
                     /locus_tag="MB022_000539"
     CDS             complement(5083..6093)
                     /locus_tag="MB022_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4465566.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6273..6617)
                     /locus_tag="MB022_000540"
     CDS             complement(6273..6617)
                     /locus_tag="MB022_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4465567.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6610..7044)
                     /locus_tag="MB022_000541"
     CDS             complement(6610..7044)
                     /locus_tag="MB022_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBT4465568.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7182..7919
                     /locus_tag="MB022_000542"
     CDS             7182..7919
                     /locus_tag="MB022_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465569.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7912..9123
                     /locus_tag="MB022_000543"
     CDS             7912..9123
                     /locus_tag="MB022_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465570.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9327..10109
                     /locus_tag="MB022_000544"
     CDS             9327..10109
                     /locus_tag="MB022_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBT4465571.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10179..10841
                     /gene="trmB"
                     /locus_tag="MB022_000545"
     CDS             10179..10841
                     /gene="trmB"
                     /locus_tag="MB022_000545"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBT4465572.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10939..12693)
                     /gene="ade"
                     /locus_tag="MB022_000546"
     CDS             complement(10939..12693)
                     /gene="ade"
                     /locus_tag="MB022_000546"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBT4465573.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12825..13754
                     /gene="tadA"
                     /locus_tag="MB022_000547"
     CDS             12825..13754
                     /gene="tadA"
                     /locus_tag="MB022_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBT4465574.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13711..14760
                     /locus_tag="MB022_000548"
     CDS             13711..14760
                     /locus_tag="MB022_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBT4465575.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14757..15035
                     /locus_tag="MB022_000549"
     CDS             14757..15035
                     /locus_tag="MB022_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBT4465576.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15010..15480
                     /locus_tag="MB022_000550"
     CDS             15010..15480
                     /locus_tag="MB022_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBT4465577.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15497..15799
                     /locus_tag="MB022_000551"
     CDS             15497..15799
                     /locus_tag="MB022_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465578.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            15816..16187
                     /locus_tag="MB022_000552"
     CDS             15816..16187
                     /locus_tag="MB022_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBT4465579.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16159..16518
                     /locus_tag="MB022_000553"
     CDS             16159..16518
                     /locus_tag="MB022_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465580.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16690..17697
                     /locus_tag="MB022_000554"
     CDS             16690..17697
                     /locus_tag="MB022_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4465581.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     TDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17722..18906
                     /locus_tag="MB022_000555"
     CDS             17722..18906
                     /locus_tag="MB022_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBT4465582.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19343..20926)
                     /locus_tag="MB022_000556"
     CDS             complement(19343..20926)
                     /locus_tag="MB022_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4465583.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21504..21647)
                     /locus_tag="MB022_000557"
     CDS             complement(21504..21647)
                     /locus_tag="MB022_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBT4465584.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21869..22348)
                     /locus_tag="MB022_000558"
     CDS             complement(21869..22348)
                     /locus_tag="MB022_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4465585.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22421..22705)
                     /locus_tag="MB022_000559"
     CDS             complement(22421..22705)
                     /locus_tag="MB022_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465586.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22783..22977)
                     /gene="ssrS"
                     /locus_tag="MB022_000560"
     ncRNA           complement(22783..22977)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MB022_000560"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23049..24335)
                     /locus_tag="MB022_000561"
     CDS             complement(23049..24335)
                     /locus_tag="MB022_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBT4465587.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24473..25143
                     /locus_tag="MB022_000562"
                     /pseudo
     CDS             24473..25143
                     /locus_tag="MB022_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
     gene            25109..25597
                     /locus_tag="MB022_000563"
     CDS             25109..25597
                     /locus_tag="MB022_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBT4465588.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25610..26557
                     /gene="prmA"
                     /locus_tag="MB022_000564"
     CDS             25610..26557
                     /gene="prmA"
                     /locus_tag="MB022_000564"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBT4465589.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26558..27322
                     /locus_tag="MB022_000565"
     CDS             26558..27322
                     /locus_tag="MB022_000565"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBT4465590.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27555..29768
                     /locus_tag="MB022_000566"
     CDS             27555..29768
                     /locus_tag="MB022_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBT4465591.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29779..30225
                     /locus_tag="MB022_000567"
     CDS             29779..30225
                     /locus_tag="MB022_000567"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBT4465592.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30396..31655
                     /locus_tag="MB022_000568"
     CDS             30396..31655
                     /locus_tag="MB022_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBT4465593.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31744..32690)
                     /locus_tag="MB022_000569"
                     /pseudo
     CDS             complement(31744..32690)
                     /locus_tag="MB022_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            33084..34376
                     /locus_tag="MB022_000570"
     CDS             33084..34376
                     /locus_tag="MB022_000570"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBT4465594.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34391..36160
                     /gene="aspS"
                     /locus_tag="MB022_000571"
     CDS             34391..36160
                     /gene="aspS"
                     /locus_tag="MB022_000571"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBT4465595.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36269..37144
                     /locus_tag="MB022_000572"
     CDS             36269..37144
                     /locus_tag="MB022_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4465596.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37410..39293
                     /locus_tag="MB022_000573"
     CDS             37410..39293
                     /locus_tag="MB022_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4465597.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39368..39652)
                     /locus_tag="MB022_000574"
     CDS             complement(39368..39652)
                     /locus_tag="MB022_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4465598.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39642..40376)
                     /locus_tag="MB022_000575"
     CDS             complement(39642..40376)
                     /locus_tag="MB022_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBT4465599.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40505..41128
                     /locus_tag="MB022_000576"
     CDS             40505..41128
                     /locus_tag="MB022_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBT4465600.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41320..42441
                     /locus_tag="MB022_000577"
     CDS             41320..42441
                     /locus_tag="MB022_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBT4465601.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42448..45576
                     /locus_tag="MB022_000578"
     CDS             42448..45576
                     /locus_tag="MB022_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBT4465602.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45595..48789
                     /locus_tag="MB022_000579"
     CDS             45595..48789
                     /locus_tag="MB022_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBT4465603.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48935..49642)
                     /locus_tag="MB022_000580"
     CDS             complement(48935..49642)
                     /locus_tag="MB022_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4465604.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16296 a         8797 c        10426 g        14472 t
ORIGIN      
        1 atgtcacaat ctttttttcg aataaactta ttttttgaag tcggccattt atcgtaattt
       61 ctttgcataa tacgaacaaa ttttcggtta ttgatgaaaa aaaacattga aaatggtatg
      121 ataaaagaaa aaaaggagga aatttttaat ggtgaaattt atccatgcgg cagatttaca
      181 tatggatcgg tcatttgaag gactgacaac tcttgataaa actgtacagg aaaaactatt
      241 gaagacgaat ctgaccgttt tatcaaatat cattgaacag gcaataataa ataacgttga
      301 ttttgtttta cttgcaggag ataattttca tcaaaatcgt ccttcattaa aaatacagaa
      361 acatttttca gaacagatga aacggttaga aaagaaccat atacctgtat ttataatatt
      421 cggaaatcat gatttctatc aaaaagagcg gtattggttc tcctttccta aaaatgtcca
      481 tttatttaca tccgaaacgg tagagacaaa aaaaataacg ataaaaagtg gagaaactgt
      541 ctctctcagt ggatttagtt atcgtcaacc gtggattcaa aaagacaaag tgatggaatt
      601 tccttctaga gaactcacag attaccatat tgggttgtac cacggggaac ctggcgtctc
      661 tcaaaaagga aactatgctc ctttccaacc ctcaaagatg caagaaaagg gatacgatta
      721 ttgggcttta gggcatatcc atgtgccaac ggtcttaaat gaaaagcaaa caattgttta
      781 tccaggtgcg cctcaaggtc atactaaaaa ggaacaagct tctacatcta ttctactagt
      841 tgaattaagt aaaggaagtt gccaaataca tccgattaaa gtagcagagg tctattggaa
      901 gacaaaggaa atatcattga ggaaagcaag aacgacaaaa gaggtcattg ttcacattcg
      961 tcagcagctt tcaaaagtag aggatggatt tagtttaatt gagataaaat taaaagatta
     1021 tgaccatctc aatacagatg ttttggaacg gatacaatca ggagagctat tagactattt
     1081 gttagaggaa ttctcagata ggataaatga tttctttatt tggcgtattt ctctcataga
     1141 gaatactttt tatacgaaga ccccacttgc tgcttctgct aaattgatgg aacagttatt
     1201 tcaatactat cagacacctc aagatttcca acaaatttta aatgaagtat atagtcatca
     1261 agaagcggct agaatattga gcgaactgcc ggaatatcaa gaagaaacac ttgaaaaagc
     1321 caagcagctg cttaatcaag attttttatt tgaggaggat caggaatgaa gattttaagg
     1381 atcgagattg ctgcttttgg aaaatggcgt caaaaaagtt ttgactttta ttcaggcaat
     1441 cagctgatct atgggggaaa cgaagcagga aaatcaacga tttatcaatt tattcaagca
     1501 atattatttg gtttcccttc aaaaggccgt aaaaaaaagg attatacacc aaaagacggc
     1561 tcagcctatg gcggcaaaat atggcttaaa catcctgtat atggtgaatt tgctgtcgag
     1621 cgctacaagc agcaaaatcg cggaaaatcg aaggtgtggc tgggggatca agtgggaagt
     1681 gatgagttac tagaaaaact gatcgcacct ttgacgaaag agttattcca gcaggtattt
     1741 actttccagc aagagcagtt gatggaacta gatcatttga gagaaaaaga gctacatgat
     1801 gcgctgattt ctttaggcat cacgggcagt tctcaagtat tccaaaagcg gcaagaatat
     1861 tatcagaaag cacaacaatt attcaagaaa aaaggccaaa aattactaat caatcaaaaa
     1921 ttaaatgaat ggaaagagct gcaagaaaaa attcatcaaa aacaagaaca ggaagcttat
     1981 tttggcaagt tgatccaaca agaacaggaa tacgagtcgc aacagcagca gttattggca
     2041 caattaaaag aggcgaacaa acaattttca ttagccagac agcaagaact taatttttct
     2101 ttatatgaag aatggcaatc tctcaaaaaa aacagcacta caggtcttcc tgatgagcaa
     2161 cttcttttag atctggagtc tttctccaaa cggtatcagc aaacaaatga gcgtttggaa
     2221 gaattagaag caacactgga aaagcatagc gggatggatc agcagtcagc cagatattat
     2281 ttttatttag agcaggagca ggaactaaaa gatctccagc aacttcaatt tacagcggaa
     2341 caattaaacg aagaatggca aagattacaa attgagacta gagaaacaaa ttcagaattg
     2401 aagattttgg aacaaaggtg gcattggcag aaaaatccgt taccggaaga atttttatat
     2461 gaagaagaat ggaagcatct tctggctgaa gaaagaagga ttgctgatct agaaagccag
     2521 atttctgtgc gtctccaaat agctaaagaa cagcaagaga tgattgaagc agaagtgaat
     2581 gacttggaag agacctatcc agcattatta caaaatcagt cagagatagc accagtctct
     2641 tcatctaaac cgtatatgct gatggctggc ggagcaataa tcattgcagg tatatttgta
     2701 ccaatgccct tgaaagtgat ccttttacta attggtgcag gcatgattgg ctatttcttc
     2761 tacaaaaggc aaccaaaaat taaaactaaa aatgatttct ctgagataaa aggaatgtgg
     2821 caagaaaaac taggacaact tgatctagcc ttagctcaat tagctgaagc agaagaaaaa
     2881 caaaagagaa ctggagaaga aaaacagatt cttcagcaag aagtatatcg ttttgcggat
     2941 catcggcact taggaaagat ggatacgctc aaactcatga aagaatatgg acaggaagtc
     3001 atagattatc agaaattata tggcgtattt gagcggaaac ataaaagaga acaggaaata
     3061 caagaaaaac tacgacaaat cgaccaaaaa tttgagtttg ttaaagaatg gttaccgata
     3121 caagataaga caatttcaga aaaaatggta ttgctttccc gttttataca agagatggaa
     3181 cagatgaaat ttgccagaag ctatcagcaa aatactttat tgaagcaaga aatcaatcaa
     3241 ttgaaaaaga aacaaaaagt atggatacaa gaatacagcg aacaattgac tcaagcaggc
     3301 ttatcttatc cttcggaagt acctgtcttt ttacaaaaag ccaaacaagc cgaacagcag
     3361 atgaaaagat acaatgaatt atcggaaatg cttactccac tatttcctga agagctgaca
     3421 aaagagaaat tatctgtaag actgcgccaa ctgaaagaaa agcaatccca actccagcaa
     3481 gaagaaaaag aagcaagtga aaatcggcaa cgactccaac ttcaagtaga agagttgcaa
     3541 agagacggca cattggacga actttatcaa caagctagcc ggcaaaaagc aaaattacag
     3601 gaattactgg aggaatgggg agcacttgaa atcgccggga cattcttagg tgatctttca
     3661 acagagatga gtgaaagaca attaccccag ttgttaagag cagcaggtga ttatttaggt
     3721 attttaacaa aaggaaatta tcagaagata ttactgaaaa atgatacact gactttaaca
     3781 gacggatata cagaatttcc catctatgag ttatctacag gtacgaaaga tcagctgatt
     3841 atggcgatga gatttgctta cctctcatta gaagctaacc gttcgatatg cccgatcatc
     3901 attgatgatg gctggcttca ttatgatcat cagcgaaagt atcaattagc tgaactttta
     3961 gctaaatttg gagaaaagta tcaaataatt tgtttttctt ctgataaaga aatggtaagc
     4021 tattaccatg agatgaacca aatcgtaaaa caattagaag gagcaaataa tgaaaaaaat
     4081 tcgtgattta gtaatcgatg agcagtttga acaatttgtc ctgatcaaaa gtgcagatgt
     4141 aagaatcgct aaaaacggaa aaaaatttat tgcttttact ttccaagata cttcaggtac
     4201 tatcgatggt aaatactggg atgcttcgga ggaggaaatc aataaattta cagccggcac
     4261 agtcgttttt ctaaacggta aaagagaggt ttatcaagga aaccctcaag taaagattct
     4321 tcatatgcgc ttaacaaggg tagatgagcc aggagagcct agtttataca tggaaagagc
     4381 ccctttgaag aaagaagaaa tggaagaaga aatcaatcaa ttgttgtttg agatcaccaa
     4441 tgcgcattgg aatcgtattg ttcgctatct acttaataaa tatcaaaaac aattttttga
     4501 gtttccggca gcaaaaagaa accatcatgc atttgccagt ggattagctt atcatacttt
     4561 gacaatgctt cacttaggga aatcaatcgc cgatgaatat cctgagctga atcgagcatt
     4621 gttgtatgct ggtatcattt tgcatgattt aggaaaggtg attgaacttt ccggtcctat
     4681 gtcgacagaa tatacgcttg caggaaattt gatcggtcat ctggtattga ttgacgaaga
     4741 aataactaaa gcctgtcttg cgttgaaaat cagcgaagct gacgaagatg ttcttgtatt
     4801 acgtcatatg gttttagcac accatggctt attagaatat ggctcacctg tacgtccaag
     4861 aataatggaa gcagaaatat tacatcaaat cgacaatatc gatgcttcga tgcagatgat
     4921 gttgacttcg attcgtcaaa cagaaccagg aaaatacacc gatcggattt tcggtatgga
     4981 caaccgaagt ttctatgtac caaaagattt atagcaaaaa tttatttttt tcattcaaaa
     5041 caaaaaaagc gagacataag tcttcgcttt ttttgttttc ttttattttg atgaatcagt
     5101 tgtttcttca gttgaagatt cttttgttga atcagttgat tttgtgtcag aactctttgt
     5161 tgaagctgta gtttcacttg agtctttcgt acttgatgaa gttgttgtga atgcactcaa
     5221 cacattttca aaggcatcat ctttgatttt tacatttgca tcttttaatt cttcaccgat
     5281 tactttagtt gtgaatgcat tgtcacttag tttagtttca gtagcgatat cttttagttg
     5341 atctttgtat ttatccatgt cattgccttt attttggttt ttcaccattt tgacaacgta
     5401 gtattctgtc gcataagaag taggatttgt tgtagtaata acatcggaga tctcaccgtc
     5461 tttcaatttg aaagctgctt cttttacttc tgcaggaata gttgttgtcg ttgagtcgaa
     5521 tttaacttta ccgccatctt cttttgtctc tgtatcagtt gatttgtctt tggctaattt
     5581 agagaaatca tctccatcat ccgctgattt tttcacatct tttgcttcat cttcacttga
     5641 taatttgatg atttgtgctt ctacttctgg atggaaagat ttccaagcag ttttcaaatc
     5701 gtcatctgta atatctacgt gagctttcaa gccagcttca aaagctaaat tgttacgaat
     5761 atattcttta taagtatctt tcgtatatcc agcagcttct aattgtgatt caaaagtatc
     5821 acctaatgat ttcgcttgtt tgtcgtattc tgcatccact tgcttatcgg ttactttatc
     5881 gccatatttg ttattgaata ctttataaat gatcatccgt tgtactaagg attggttcga
     5941 tgattcaagt tttgcttcat catagaaatc agatacagtg atcgttccgc ctttcatcgt
     6001 tgcgatatct ttattagtat cgcctgaaca agctgctaga gttaaaacag ccaacgcact
     6061 agtagctgcc aaaataattg attttttttt cattaaaatt tgcactcctg taattagtta
     6121 ttatttttat atattaacta taccatatcc catttccttt aaaaacgttg tcgtggtagt
     6181 ttcaaacaaa attcaaaatt tcatcacatt cctaggaaat acgcaaactt ttctcactta
     6241 tatagttaaa tctgttgaga aaataatcat tattatattt gttcaggata ctctggcaga
     6301 tcagctttta gtttttctac ttgttcattg atttgtgtga tacgtggttc ggcttggaat
     6361 ttaaaatctt cgatatcttt tttgattgct tcttggaaag caggaatcac agattcagct
     6421 gtttcttttg tttcgatcaa tgcgctttta aaatgattca ggctgttatt taaatcattt
     6481 gtcagagttg ttgcttcttc caattcttcg atcagtttct ttcttgtttc attcccgctc
     6541 ctgggcgcca ataacagacc tccgacagtg ccagcagcag ctccaaataa cagtcctttg
     6601 aaaaagtttt taatcatcgc aggacacctg ctttacgatt gtttcttgaa tagcttttag
     6661 ttcttccgat ccataactat cttgatgatt gacaaagtga atagaaaaat catcctcttt
     6721 gctgtagcga gggactaaat ggatatgtga atgaaaaaca gattgatagg ctagttcacg
     6781 gttgttattc aagatattaa gacctttcat ttctggaaaa gctttttcca atgcttgagc
     6841 aatcttaggc acacgcgaaa aaacctctcc tgcaagttcg ggatcatatt caaaaatatc
     6901 tgctacatga cgtttaggca caactaatgt gtgtcctttc gttgcttgag agatgtctaa
     6961 aaaagcatag accttgtcgt cttcatatat tttatagctt gggatttctt gattgatgat
     7021 tttgcaaaaa atacagtttt ccatataaaa catcctttct ataaagaata aatacttcag
     7081 cttaacttta ccataagtta aagctattgt aaggaaaaaa acaaaatacc gtcattttct
     7141 tcgtggtata ataaataaaa ataaatggcg gaggaaaaag aatgagttta attatcgatc
     7201 atgtcacagg ggggtacgga catctccctg tgttaaaaga tatcagtttc gatgtacagc
     7261 ctggagagat agtagggttg attggactga acggagcagg gaaaagtacg accatcaagc
     7321 acattatcgg tctgctggaa gcttcaaaag gaacgataga aatcaatcat ctatccttaa
     7381 agaaaaatac ggaagaatat cgcaaacaaa ttgggtatat tccagagact ccttctttat
     7441 atgaagaatt gacattgaga gaacatatcg aagtcactgc gatggcctac gatataccta
     7501 tggatatagc aatgaaaaga gcagaaaagt tgttggagac ttttcggtta tctaatcgtc
     7561 ttgactggtt cccagctaat ttttcaaagg gaatgaaaca gaaagtgatg atcttatgcg
     7621 cctttttgat cgagccttct ttatatatca tcgacgaacc atttttaggc ttggatccac
     7681 tagctatcaa tgcattgctt gaattgatgg tagaaatgaa aaaagaagga gccggtattt
     7741 tgatgtctac gcatatttta gctactgcag aaaaatattg tgatcgtttt gtggtcttac
     7801 ataacggaga gatacgagca gacggttctc ttgagatgtt gagaaaagaa tttcagctgc
     7861 cggattcttc tttggacgaa atctatattg ctttaacaaa ggagcaggca catgagtagt
     7921 ttttttcaaa aacgtttagc aagacatcaa aagaaaatga tgaaatatat gcgttatgta
     7981 ttgaacgatc attttgtttt agtttgtctt ttcttgattg gcggtgtggg attatattat
     8041 tctaattggt tgaaaaccct gcagccaccg tttgcaattg gagggctcat aatctctgta
     8101 ttttggatgc tctgtttatt cgtgggtaag atggctactt ttgcagaggc agcagatatt
     8161 gtttttttac ttcctaaaga aaaagaaatg caaacctatt tggaacgagc ttttcgctat
     8221 tcttgcattt ttccgttgat ctttttgata ttggcatgcg gattggccat gccattagtg
     8281 gtggttagta cggagcaatc cttttcgatg tttttcgtct atctgatcat attatggagt
     8341 ttgaaattca gtcatctgca aatcaagaga gcagaattgt ttcgactaga tcaacaagtt
     8401 attcgttatt ggaaattagg ctggtttttt tcttctctga tctttttgtt tctatcattt
     8461 tattggctaa tatgggcagg aatagcaggt gcattagtac aggcaagtct atattactgg
     8521 tttttgtgga agcaacaaga aaaaggattg gattgggaga aaatggtgca tgtagaagaa
     8581 cataggttgc atcagatcta tcaatttatc aatttattca cagatgttcc tgaaattacg
     8641 gcaaagatca aaagaagacg ctatttggat cctatattga ctcatattaa aagagaatca
     8701 aaaaacactt atctttactt gtttgcaaga agattggcaa gaggttctga ttttagtggt
     8761 ctttatttcc gattagtaat tataggtggg ttattgattg ccggagtagt cgacttttat
     8821 tttagtctga ttatcggttt cttattttta tatttgatcg gtttccaact gttaccttta
     8881 gcgaaccagt tccgctatat ggttctcgtt cagctttatc caatccataa gaatcagaag
     8941 aagcaggcga ttcagtatct actcacatgg gtattaatta tcgcgtcggt tatttttggt
     9001 ttgattgctg cagtcgtatt agatgggcag cagcgatggg ttccgctagc ggcttatttg
     9061 attgttacta tgctctttgt taaactatat gtaccaactc gtttgaagaa gatgtcagac
     9121 tgaaaacaaa gctgtgtagg gtaaaaatga gaatgaaaaa tagagcgttg acagtaagaa
     9181 tgctgttcgt tctatttttt ttgagaaaag ctggtttttt gaatggcaat taaatgaaaa
     9241 agaacgtata tgtgcttgac gtgtagtgtt caggcgacta gaataataag tactgcaaaa
     9301 caaagagtaa gaggacggga gaatgcatga tggagtttca gttagaccaa gagtggcggt
     9361 tacgtcccat caaaggcgac acaggaaaaa catacatagg catgcgtgat gaggacaaag
     9421 tctttattaa acgaaacacg actccgatgt tggctgctct ttcaagggaa ggaattacac
     9481 caaaacttgt ttggacaaaa cgaacgggga atggcgatac tttgacagca caagagtggc
     9541 tggaaggtcg cttgctgact cctaatgaga ttggtcaaag aaacgacgtg attgatgttt
     9601 tgtaccattt acatcattca aattctctta aaaccatgct gacccgtatc ggtggtcgtg
     9661 tcatgacgcc ggagaagatg ctgcaaaaat atgaaaaaga attaccggaa gaactccaga
     9721 aaaatcaatt tctgcaaatt gtccatcgtt ttcttcgtga gcagatcccg tcaatagata
     9781 aggaaaactt atcagtagta cacggagatg taaaccatcg taattggtta gtatgtaaaa
     9841 attatttata tctagtcgat tgggattcaa tcatgtttgc agaccctgcg gtggatattg
     9901 caacgatttt aggaaattat gtgccactgt ctagctggaa tcagtggctg atcagttatg
     9961 gcatacgccc gactgacgaa atgcttgaaa aatttcattg gtatgcggcc atgaatttgc
    10021 tccaagaaat caccagatat tgcaaacgtg aagatcatcg acggatgaac gaagaaattt
    10081 tacaattaaa acgaattttc agtggctgag gtttggccca aactctgcgg agatttggcc
    10141 agaccttttt aattagagga aaggaatgga gataaataat gcgtgtccga aatcgtccgg
    10201 gagcagcgga aatgttagcg gctcacccga attttgtaat tagtgatcca acattgtgga
    10261 agggaaaatg gaatgaattg tttgaaaacg atcatccaat ccacattgag attggtatgg
    10321 ggaaaggcca gttcatcact ggaatggcaa aagcccatcc tgaaatcaat tatattggag
    10381 tagaaatgca ggtcagtgtt gtttcgattg cactagacaa gttgatcgaa caaccgcttc
    10441 ctaacctgaa acttctacat gtggatggat cagcgttgac agagtatttc gctgacagcg
    10501 aagtagatca aatctatctg aatttttccg atccatggcc aaaaaaacgt catgaaaaac
    10561 ggcgattgac atataagact tttcttgctg tagatgaaca gattcttcgt ccaaatggag
    10621 aaatccattt caaaacagat aatcaaggat tgtttgagta ttctctggca agtttttctc
    10681 aatatggcat gatcctcaaa caggtatggt tagatttaca tcaaagtcaa tttgaaggca
    10741 atattatgac ggagtatgaa gaaaaatttt cttcaaaagg tcaacgtatt tatcgagtag
    10801 aagctcaatt tcaagataag accaaaaaga aaagtctgtg aaactaactt tctgctttca
    10861 taaaaacatt aatatccata aataaatctc agaaaatgaa cattacagat gtaatgtatt
    10921 ttctgggatt tatttgattt aaaatgtatt tatttcatcg gtttttccac aaggtggaat
    10981 caattggaag cgagaaaatg aaaataatcc tttgtcggtg atcttcaatt cgggtatgac
    11041 agataatgtt aagaaagaaa gtgtcaaaaa aggatcaaag gttttagctg ctcctagttg
    11101 atgtgcttta gttattaatt cattcaactg ttcatagacg atcgaaaaag gttcctgtgt
    11161 catgattccg cctataggaa gagaaacaca agctatttcc tgctgtttgt tgacaacggt
    11221 catcccgcca cctttttgaa tcaaaagatt agcagcatac agcatatcct cgtcgtttgt
    11281 cccaacgaca actagattat gcgagtcatg ggcaatcgtt gtcgctatcg ctccttgttt
    11341 cagctgaaat cctttcacta tgcctaatcc aatgtttccc gtttgatggt gacgctcgat
    11401 caccgcaatc tttaattggt ctttttcgat tgaaggaatg aatcgttcgc cttgtcggtc
    11461 cactttttct accaaatctt tagtcactag actattcggg ataatttcga tcacatgggc
    11521 ttgtgaagaa gaaagaagca gctctaacga atgtggattt aatggttgag cgtttagttt
    11581 tggcagacaa cacgtatatg aatttgaatg atcagcaaat aaatggtctt ggagttgccc
    11641 agattgaaca actaatgttc ccttatagaa aacttcatga attggcagct tggtaagatc
    11701 gtctgttaag aagaaatcag cttgataccc aggggcaatc gcacctagtg ttttcaaatc
    11761 aaaacattct gctgcattca atgttgccat ctgaatggct tgtaatggat cgattccaag
    11821 tgcaatagct tgacggacga tatgatcgat tgatccttct tttactaaat cattgatcag
    11881 tttatcatcg gtgacaaaga agcatcttct tgaattttca ggtgtgatcg ccggtagtag
    11941 cgatgccaga tcttttgcaa cggtcccttc tcgaaccatt aaatacatac caagatccaa
    12001 acgttcttta gcttcttttg cagttgtagc ttcatgatcc gtccgaatac ctgctgcagg
    12061 ataaacattt aactcttcca tgccgatacc agctgcatgc ccgtcaattt ttttcttatg
    12121 ctggtgcatc aattggatct tttctaggat gtctgtttca ttatttgcta ctgcttgata
    12181 gttcatgact tcagctaagc ctaaaacttc gggtttttgt aaaaaagggg ctaatttttt
    12241 tgcatttaaa tttgcaccat tggtctcaaa aggggttaca ggtacacaag aaggtagcat
    12301 aaaaaagata ttcatgagtg tttgtcttgc atcttctatc ataaactcaa gacctgtact
    12361 cccagcaatg tttcctattt catggggatc tgtcactaca gtagtaactc catgaagcaa
    12421 ggatactttt gaaaattcgc ttggtgataa aagtgagctt tcgatatgca catggccatc
    12481 gatgaatcct ggcactagat attgcccatt agcattatgt acgctttttc cttcgtattc
    12541 accgattcct acgatcattc cttcgcagat tgctacatct ttttccatga tttctttcgt
    12601 aaagacgttg acgatcttag catgcttgat cactaggtca gcaggctttt ttttacttgc
    12661 tacttcgata tatttcctta atgttgtttc caatggagtg cctcccaaga taaattttta
    12721 taagtataac ataaattcgg cttttgccaa tatttttttg taaattcatt ttatttgaaa
    12781 caaaaagatc aaaaagacgt atgttttttg tggaggggag aataatggaa ataaaacgat
    12841 tatcggatca aatgataagt gaaagttttg ccaaacatgc ccaagacgta tatatattgc
    12901 cagcttcaga agtattttac atctattttc gctatggaaa aaaacgaaca ttctggaaga
    12961 aagtctcttc ggcagatgga cagcaactga tctcaagatt taagtatttg ggacagatgg
    13021 atatcggaga aaaaagaaaa gctcagttag gtgcatttac ttattcttta gaaaaacagg
    13081 aagttcgtct gcggttgtct tctgtaggag attaccttca acgagagagt ttagtgattc
    13141 ggctattata cggtactttt gaaacacccc atcgttgctt tagttctcga gattttgcaa
    13201 ctattttaca gaaaaccgtc agacgtggat tatacctttt cagcggacca gtcggatcag
    13261 gcaagacttc tctaatgtat cggttggctt tggaagaaaa attgcaagtg attactatcg
    13321 aagatcctgt agagatagaa gaaccgtttt ttctgcagct acagatcaat ccgaaaatag
    13381 cacagactta tgatacactt ttgaaactcg cattacggca tcgtccagac ttgctaatca
    13441 ttggcgagat aagagatgaa tcaactgcac atgctgcggt aagagcagca ctaacagggc
    13501 accgggtttt tgcaaccgta catgcacgca atcttgaagg tacattggtc agaatccatg
    13561 aactgacaaa gaaaaaagaa gagctatctg aatgtttagc tggtgtcatt tatcaagaac
    13621 ttttggaaga tgccaatcac catccttctg ttttatggag ttatcaattt atggaggaag
    13681 aatcagtaaa aaaatcatgg gaggaaagtt atgaagaagc tcaggaaaat atttggcagt
    13741 cagaactatt ctaaaggatt aacaagaagc cagcaaaacc agtttgcaca aaccatggga
    13801 gaactgcttt tatctggttt tagtctgcaa gaatcccttt atttattact cacgatccgt
    13861 tcgtttccac ctgttatttt ggcaaatatc caacatcagc taaaggaagg gcaagcgttt
    13921 agtgaggtac tttcgcaaat aggatatgga gcagagcaac tggtacagat cgagttagct
    13981 gaacgtcatg ggaatctgcc gacaactttg ttggaaattg ccaaacagtt tcggcttgtt
    14041 gaaggatttc gcaatgattt gaaaaagctg cttgcatatc cgatgctatt gctcaccttt
    14101 ttgctaggta tcttgtttag tatgcggttg ttcgttttgc ctcagcttat tggttcagga
    14161 atggtcatgc aagagcattg gggtattcag ctgatacaag tctttcattg gtatgttcta
    14221 ctattcatcc tagcggttac tctattggct ttactagtaa ggtatcgtct gatgaagttg
    14281 tctcctgttg atcagagtga atggataagc agacaattat tgattggacc gatatattcc
    14341 ttgtatcaat cggcttattt gtcactagag attggaaagc tttttcatga aggtttcgaa
    14401 ttaaggcaaa tcatagtctg cttaaaggaa acgaaacaag aaagtctgat tcagcatctt
    14461 gcctctaagt tgattcaagg attggaggca ggtatgtcat tggctgagca gttccagaat
    14521 tatcgttttc tatcagaaga attcagtaaa attgttttac agggagaggc aaagggaagt
    14581 ttaggtaaag aactgatttt ttacagcgaa tggacaagaa aacagctttt caaacgtctt
    14641 catcactgga tgcattttat tcagccaatc atttttttgg gcgtagcggc tttgatttta
    14701 ttagtttatg ccgctgtttt attacctgtt tacggaagca tagaggaggt tttgccatga
    14761 aaaagagaaa aatatcttat tatagcggtt ttacattgat cgaaatgcta atcgtcttac
    14821 tgatcatcag tgtattagtg ttgttgttcg tccctaattt gtcgcgttat aggaatcatg
    14881 tagaccaaga aagtcgtgaa gcgatcatcc agttggtaga cacacaaaaa gagctctatg
    14941 cgcttcaaaa taatggaaga gttcctactg tggaggaatt gttgaatgaa ggctatatca
    15001 aacgagaaca tgcagaaatc tatcagcggc cttgaaggta aggttttagc atcaggatat
    15061 acgttgtttg aatcactctt actgatttta ttacttacaa cgatcctttc ttttccagtt
    15121 ctcgcatttt ctgcatggaa gaatgaattg gcagtctatc aatttttcgg ccaatttgaa
    15181 aaacgaatct attctacgca aaaaatcgct attgtgaatc agatccaaac aggcttttat
    15241 tggaatagtg aagagaatca aatcattttt catgttccga atccagataa aataaattgg
    15301 gaagtattag atattcctga tgagattacc ttgaaacgcc atgcttctat tacttttgct
    15361 gctagaaccg ggaatgaaag ttcactaaaa gcttatcaat tttattggga agaaaaagag
    15421 cggacgatta cgtatcaatt ccagataggg agtggtcgct atacaaaaag aattgaataa
    15481 aacaggattt gttttgttgg agtcccttac agcgcttatg atcgcttctg tctgtgtcta
    15541 cttagtgagc tttggccagc tgtttttgct tcaggcagaa aagaaaagtg gggaggaact
    15601 gctatttatt cgaatgattt atgaagatat caagacgcag cggatgtatc acaaaagacc
    15661 agagcgaagg gaagaaatga tcccatacga tatccaaatt gtcgataata atggagtaat
    15721 cgaaaaagtg tttgtcaata tggagaaaga agcgatcagt attgaaaaaa caagtaattc
    15781 cagcatttac gctgattgaa tgtctaattg gactgatgat attgagcagt ctattgctca
    15841 cattcaatct tttgattcag caaaccatcc aagtaagtga atttttgcag cgtaaagatc
    15901 aaaaagagtg gctaatattt ttagcacaat tagaagaaga actcaagagc agtcacaatg
    15961 tctctgtgaa aaatcagcaa ctgcactaca cgatcgagga taaacagtat atcatcgaaa
    16021 gatatcaggc aatgatacgt aaaagaagaa cttccggcgg acatcaacct atgttaacaa
    16081 gtatcactga gcttcaatta ttggaaaaag aacacacaat ttctttttat gtacactttg
    16141 aaaatggaga agatggatat gccatatgga caaaaaacga tcagtagaaa atgttataag
    16201 ggctctattt tattttctgt cttgctttta tttcttttat gtacgttttt attgatggca
    16261 gcattagaaa gctaccgttt gtcaatggat cttaatatac gaatcaaaaa ttattatata
    16321 gcaaaaataa tgaagcagat ggctttatcg gaacagtcta cgcttgccga aagttcggaa
    16381 ggagtttttt actacacaac tggatctgta acatatcagt gggtacaaca gtccctattc
    16441 ttagaggtgg aagtctcacc gtttatcttt cggttcacag aagaagtaaa aaatgataca
    16501 gatactagta cagaatgatg gaatgttcgt gaaaacgccc tcttttctgt tataacactg
    16561 tttttaataa aaaaatgttt gccgtttcac aaataaaaaa cagtgaaaac gattgaaatt
    16621 ttgtcaattg ttcgatagaa ttaaacaggt gaaaaaattg ttataatata tctagaaatg
    16681 aggtgcacca tgttaccaga aaaaatggaa caagggttta atcaaaacct ggaagctatt
    16741 cagcttttac agaatgcatt gggaacttcc tttttagaag cctatgtcga aaatgcagaa
    16801 aatttaatag atgattatca agtacgtgtg gtggatggag tgccgacaaa agagacaacg
    16861 caacggatca cagctcttta tgaagagttg aagaaacttt ctttcgagcc agaagaatgg
    16921 cgcagactgt ctcaattact tttattgaag ggcagccaga cagaacattt gcaggcgaac
    16981 catcaactca ctcctgatag tatcggtttt ttatttgttt ttcttattga acaactatac
    17041 acaaataaaa aagcaccggt taagattctg gatattgcgg caggaatggg caacttgtta
    17101 ttaacagttc ttttaaacct atcgaatgcg gggtaccaaa cagaaggaat tggtgtggat
    17161 attgatgata cattgttggc tgtcgcagct tctacaagtg atttgacaca agcaaatgtt
    17221 caatactttc atcaagacgg gttgcaggaa ttattaatag atcctgtcga ttttgcaatc
    17281 agcgatttgc caatcggtta ttatccgaat gacgaaaaag caaaagagtt tttaacaagt
    17341 accgaagaag gccatagtta tgcacatcat ttattgctag agcaatcaat gaaatatgtg
    17401 aagccggatg gttttggttt gtttttgatg ccgagtggat ttttagaaac ggatcaaagt
    17461 gaagaaatca aaaaatggtt taaagaagaa ggttatcttc aaggaatgat tcaattgaca
    17521 gatgaattgt tccgtaataa acaatcgcaa aaaagtattt tgatcttaca gaaaaaagga
    17581 ccacaagcac aacaagtaaa agaagtcttg cttgtgaaac ttgcttcatt aaaagaacca
    17641 gagaaggtga cggaattttt caacgagttt aaaaactgga aatcatctgc tctgtaatca
    17701 ttcaacgaga aggagagtca tatgtctaaa acaattgcaa tcaatgctgg gagttcaagt
    17761 ttgaaatggc aattatatca aatgccagaa gaaaccgtag ttgcgaaagg gatcgtcgaa
    17821 cgtatcggtt tacaagattc tatttttacc atcaagtacg gcgataatca aaaattcgaa
    17881 caaatcatgg atatcgaaaa ccacgaagta gcagtaaaaa tgctgttaga tcagttgatt
    17941 gaactgcaaa ttttaagctc ttatgatgaa atcaccggag tagggcaccg tgttgttcac
    18001 ggaggagaaa cttatgggga ttcagtagtg atcgatgaga aagtaatgga ccgcattgct
    18061 gaattagcag aatttgctcc tttgcataat cctgcaaact taatggggat caaagcgttt
    18121 aaaaagattt tacctgacat tttgagtgtg gctgtattcg atacatcttt ccactcaaca
    18181 atgccaaagc aaaattattt atatagctta ccggtgtctt attatgaaga tttcaaagta
    18241 agaaaatatg gcttccatgg cacaagtcat cgttatgtgt cggaacgtgc ggcaaaatta
    18301 ttgaacaaac cgatcgaaga actaaaaatc atcacttgcc acttaggaaa tggtgtatcg
    18361 atcacagcag tcgatggagg taaatccatg gatacttcta tgggattcac accattagct
    18421 ggggtgacaa tgggtacacg ttcaggtgat atcgatccag ctgttctccc atatttaatg
    18481 gaaaaattag agattgatat tgatgaaatg atcaatgttt tgaacaagaa atctggtctg
    18541 ctaggcctaa caggattgtc tagtgatatg cgtgatcttg aaaagaacta tgaaaaagaa
    18601 catattcgtt tagcttatga cattttcgtg gaccgcatcc gaaaatacat cggcggttat
    18661 gtgacaacga tgaatggtgt tgatgcaatt gtctttactg ctggtatcgg cgagaatgac
    18721 ggacatgtac gtagtgaaat catcaaaggt atgtcttggt ttggttgtga aatcgatcca
    18781 gcgttgaatg aattgcgtgg tgaagaagta gatatctcta caaaagaatc aaaagtaaga
    18841 gtattagtga tcccaacaga tgaggaacta atgattgccc gtgatgtgga gcgtctgcgc
    18901 ggctaagcca aagcattcgt taggataaaa gatgaaacgc atgcaagagg gaattcttgc
    18961 atgtgttttt tttgcggaga agaccgcaaa atggtaattt aacttcaatt tcccttcttt
    19021 tttacaggat cttcttaggg aattttgctt ggaaaagtgg tcaaaaggga aaagactggc
    19081 attttattta ttgattttca ggaaaatccc aatattttat tttcattcat ctttataagg
    19141 gacattacat atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt
    19201 attcttcgct ttatgtttag ctagaaaacc gatccgaaac attcattgat cgaataataa
    19261 aaacttgatt ttccacctgt actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag
    19321 ttttttgttg cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct
    19381 gctcctgatc aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc
    19441 tgactgctag gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat
    19501 agctgtcgag gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga
    19561 gtttcagcaa cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca
    19621 catcgctttg ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag
    19681 tcaatgtgat ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg
    19741 gcaaataacc gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct
    19801 caccgagtaa taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta
    19861 aatgatctcc acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact
    19921 gctggtgcaa gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc
    19981 tttgcagccg atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc
    20041 gttccagttc tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac
    20101 gaaaacgacg aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt
    20161 ttgtcagctc agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat
    20221 actcaatcaa tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt
    20281 gccgatcatg agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc
    20341 agcgaatact gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat
    20401 cggctgctaa aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta
    20461 accgtgcttc ctgacggtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt
    20521 ctaatacgta accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac
    20581 acttctctaa gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt
    20641 tgaggtcctg tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa
    20701 ctgtaagttc ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc
    20761 tgtcgacacc ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga
    20821 taccgactaa ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg
    20881 gattcccgcc gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg
    20941 agaccactca aaaaggccat ggttatccct ggcctgtgtt atttaaactc agattatcat
    21001 gagaaaaata ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact
    21061 tgtcccgtca acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg
    21121 gtaattttca ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt
    21181 agttgtcttt tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact
    21241 taatatttat cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt
    21301 ctcgtggacc acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg
    21361 tttgataaaa aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt
    21421 aaacagctct tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg
    21481 caatggcaag cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc
    21541 ttctcgcggt acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc
    21601 atcgatactt catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa
    21661 taagctttgg catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt
    21721 tcactttttt tattttactt gaactcattg actgttttcc ctttatttta aacattataa
    21781 agaccgatga gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc
    21841 tgccgatagt tggcaggtcg tctacgtgtt aagctttggt gtggtcttta gctggcaatt
    21901 ggttttctaa atcagttcgg acaacaagca catcgcaagc tgcgtttcta ataacatatt
    21961 cagaaacaga accaataaat aaacgttcta cagcattcaa tcctgtggct cctaacataa
    22021 tcaaatctac ttgattatct tctgggattt ctctggcaat aatttgtttt ggtgagccat
    22081 attctacgac agaggttaca ttattcaagc cagctttctt cgcattgctt tcataatctg
    22141 ccaatgtttg ctttgccatt tcagtagctt gttctgcaag cattccatca aaggatgaaa
    22201 cggtttggaa agaacgtgtg tcaatgacat gtgctaatag aagttctccg ttatttcgga
    22261 tagctacatt gaccgccttt ttaaaagcta attcagcttc atcagaacca tctacggcaa
    22321 ccatgatttt tttgtattgt tgtaacataa gcattcactc ctcaagtcaa tcgtattgta
    22381 ctcttttatt gtagaacact ttagggaaaa agagaattat ttactgcttt ggttcagcaa
    22441 ttttttttgc aaatcggata ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga
    22501 ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat acccattacg cctaaaacag
    22561 catatgcagc accgtaaaat tgtaggaatc cttgattttt atcatttttg ttgatcaata
    22621 caaaaaagat atcctgttta ttaaataaat aatagctaac gaataataat actaaagtgg
    22681 cgataatcat taaaatccta atcatgaaca aatcctccaa aataagcaac aaatagaaaa
    22741 agcaatttgc caagagcaaa ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc
    22801 gttgtttgtc ctgtagtatt gatcccgcga attcaagcag gtgggtccca tggtcgtagc
    22861 ttcgaactac caaatgataa ggcatgttac cagcagtgac acaagacggg tcccaagata
    22921 tcaataaaaa tgttcggtca acacacaaag aggacaattc gaacatcaca gatttcccac
    22981 ctctatagta gcatatgaat ttaaaaagag caatgagaga gctcgcagaa acagaaccgt
    23041 tttcttgtct agttttttgg aggatgcttt ttccactctt ttatacggta atactgttga
    23101 cccagagcct gttcatattt ccctgtatct tttggctgat aatactgagc attcttcagt
    23161 ttatctggta aatattgctg attgacccaa gcctgatcaa aatgatgagg atactgataa
    23221 cccacgccac ggttgagtga tttcgcacct ttgtaatggc tatctctcaa atgatcagga
    23281 acatctccag ctttgccctc tcgaatatca gcaagtgctg catctaatgc catgtaggca
    23341 gaattggatt ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc
    23401 tcaggcaatc ccaatttttc agcagctaat acagcgttca ctgtacgagc cgcagcagca
    23461 ggattaccta aacctatatc ttcataaccg ataaccatca gacgccggca aatacttgcc
    23521 agatcacctg cctcaactaa acgggcgaga taatgaagtg cagcatccac atcacttcca
    23581 cgaatcgatt tttgaaaagc cgaaataacg tcataatgag catctccatt tttgtcatgg
    23641 gtaagagctt ttctttggac acattcttca atgatcgaca gtgtaagatg aatccttcct
    23701 tctttatctg aaggagtcga tagtgtagcc agttccagcc cattcaatgc actccgcaaa
    23761 tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt cgtctaactg gattgcttgt
    23821 tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga ctgccaactg gatgtcttgt
    23881 tctgttaatg gtttgacttc aaagatctgt gtacggctgc ggatcgctgg attgatggta
    23941 atataaggat tctcagtagt cgctccgatc aagatgattc ggccactttc taaatgagga
    24001 agcagaaaat cctgttttgt tttatctaat cggtgtactt catccaataa gaggatcact
    24061 gtaccactca tttttgcttc ttccgctaca acttgtagat ccttttttga atctgtagcg
    24121 gcgtttagca tgcgaaatgc ataattagta gagccggcga tcgcacttgc gatactcgtt
    24181 tttccagtac caggaggtcc atatagaatc atggaagaga gcatgcgagc atcgaccatg
    24241 cggcgtatga tttttccttc cccgactaga tgctgctgac caacgacttc atcgatcgtt
    24301 cgaggtctca tgcgataagc caatggctgt tgcataggat tttcctcctt cttgttttta
    24361 cgtcccttaa ttatatcata tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt
    24421 gttttcatag cttatccaac taatattgtg tatgataaag taggtgagaa aaatgacgga
    24481 taaacaaaag atagatgaat ttttttggaa taaaacgacg ccagaagtgg cacgggattt
    24541 attggggatg tatttggagc atgacacacc agaaggaaga ttagctggat atattgtaga
    24601 cgcggaagca tacctaggtc cagaagacga ggccgcccat agctatggtt taagaagaac
    24661 accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc tacctttaca cgatgcatac
    24721 tcatcggatt ttgaacatca ttactcagcc agaagggatt cctcaaggag taatgatacg
    24781 tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg
    24841 tccagatatc agcaacggtc cgggaaaatt agtggaagca ttagcaattc ctcaagaact
    24901 ttatggtcag tctatagcag atagttcgtt acgcttagtt tttgagaaaa aaagacacca
    24961 aaaaagatca atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag
    25021 ccgttgcgtt ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc
    25081 gagaaagatt ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga
    25141 tgaaataatt gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa
    25201 tcatacgatc gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga
    25261 tgatgcagaa gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct
    25321 ttttaatcct cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga
    25381 aggaaaaaaa gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt
    25441 attagctcaa gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt
    25501 attcgagctt cactgggata atcaacaact agcagaatta gtagaagtaa aacagcaaaa
    25561 tgaagcagat acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa
    25621 tgaagtaaaa gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga
    25681 ggcaggggcg agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga
    25741 tcaatatggt gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt
    25801 catggcttat tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg
    25861 tatcacacaa ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga
    25921 agtagaggaa agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac
    25981 gcgaatgctg acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa
    26041 aattatcaca ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt
    26101 gacattgaca gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac
    26161 tgggtcgggc gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt
    26221 tgatttagac gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc
    26281 caaagatgtc catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt
    26341 aatcgttgcg aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt
    26401 actgaagcaa acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt
    26461 tctggatgcc atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg
    26521 gtatgcaatt attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga
    26581 atcatatgaa tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg
    26641 ggttatgagg atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat
    26701 tgttgcagaa atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga
    26761 agaaaatcaa aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga
    26821 aaagctggat tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt
    26881 cccggcatca gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg
    26941 gcttgaaaag attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt
    27001 agagttattg gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt
    27061 ggttgcttat gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag
    27121 tgaaacaaaa ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg
    27181 cgatgaagtc gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat
    27241 ccttcgtaca gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt
    27301 aatgaatcct tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt
    27361 gaaaaaatag tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa
    27421 tcgtttgaaa aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga
    27481 ttttgggaca attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag
    27541 ggggatcgat tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg
    27601 ttagtcaata catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga
    27661 ctgctgcaca tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc
    27721 aagtcgcagg tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct
    27781 tacatgatgt tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg
    27841 atgacgttgc tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc
    27901 acgaagagca gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac
    27961 gagttatcat ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc
    28021 gagaagataa gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac
    28081 atcgtctagg gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa
    28141 atccaaaaca gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa
    28201 aatacgtaag tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg
    28261 cagaaatcta tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga
    28321 aaaagcagtt caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca
    28381 aggattgtta tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat
    28441 ttaaggatta tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga
    28501 tcggtccggc aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg
    28561 ctgaattcgg ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac
    28621 cagatggtat gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa
    28681 gctatgatgc ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct
    28741 acgtcttcac accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact
    28801 ttgcatacag tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca
    28861 aaatggtgca gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc
    28921 caaattcatt tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa
    28981 ataagatcaa acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg
    29041 aatccgtggt caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga
    29101 acaaactgca agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag
    29161 ctgtagggta tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac
    29221 gtaaagaaca aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga
    29281 aaaaagaacc tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag
    29341 aaaacttact aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg
    29401 gttatatcac taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc
    29461 ctgacaaacc aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata
    29521 cacgaaaaga gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga
    29581 atgatgtact tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga
    29641 cgaataaaga taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc
    29701 taaagagtat cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga
    29761 atggatagga ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca
    29821 gtcgatcagc aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat
    29881 gaaaccgata cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt
    29941 tttgaagatg aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta
    30001 agtatctctc aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg
    30061 gaagccgcac gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga
    30121 agtcgcggca tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc
    30181 aatgatggac ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc
    30241 actagtaaaa actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg
    30301 cttttttgtc tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa
    30361 ttatacaagt tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata
    30421 tcaaaatcac agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac
    30481 gccatgaagc ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga
    30541 tctacggatc atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg
    30601 ctatgccaaa attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg
    30661 gagctgctca tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg
    30721 aagattatca tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc
    30781 aatttgaact atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag
    30841 gtatcaattt atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct
    30901 ctatgatcag cggaacgttt acttctccga ttgatcacat aaaaggtgtc gagaatcgca
    30961 aaaaatcaga aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg
    31021 gaaaagaaaa tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt
    31081 tacctctgga agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa
    31141 ctgtgtgggc ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat
    31201 taaaaaatga cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc
    31261 ctttcttccc aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta
    31321 taccagaagg cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag
    31381 ttgaagcgcc agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct
    31441 ataaagaaga atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct
    31501 gtgcaggaga atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata
    31561 agtttgagtt ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt
    31621 atccgaatag ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa
    31681 ggaagatcaa acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat
    31741 tgatcaaaat ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa
    31801 atctaaagct tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac
    31861 agacactcgt ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta
    31921 tcttttagca atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc
    31981 ttggaaatct ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag
    32041 ttcatatttt cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt
    32101 gtttttttca agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt
    32161 atcttctact acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa
    32221 gtttgacaca ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac
    32281 tgattcattt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca
    32341 gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca
    32401 aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga
    32461 tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa
    32521 gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt
    32581 cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat
    32641 ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc
    32701 tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg
    32761 ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat aagtaatatc
    32821 gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct
    32881 gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc
    32941 gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac
    33001 cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat
    33061 agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt
    33121 tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag
    33181 attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa
    33241 gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg
    33301 aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc
    33361 ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt
    33421 ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag
    33481 catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct
    33541 tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa
    33601 cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga
    33661 gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca
    33721 aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg
    33781 ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa
    33841 tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga
    33901 gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc
    33961 tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc
    34021 gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg
    34081 cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga
    34141 ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat
    34201 ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg
    34261 ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag
    34321 aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg
    34381 aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga
    34441 acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt
    34501 tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa
    34561 agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga
    34621 agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt
    34681 gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga
    34741 tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc
    34801 aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt
    34861 agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg
    34921 cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca
    34981 atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat
    35041 cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt
    35101 agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag agggattgat
    35161 tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac
    35221 ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga
    35281 attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct
    35341 tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg
    35401 aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg
    35461 gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac
    35521 agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga
    35581 taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt
    35641 aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga
    35701 attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga
    35761 agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat
    35821 cgtcttgaac ggttatgaat taggcggagg ttctatccgt attcatactc gtgagcttca
    35881 agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt
    35941 cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat taggattgga
    36001 ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa
    36061 aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt
    36121 gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg
    36181 atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa
    36241 gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca
    36301 tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt
    36361 gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat
    36421 tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta
    36481 tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat
    36541 tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt
    36601 cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat
    36661 tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat
    36721 tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt
    36781 ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt
    36841 cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat
    36901 cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat
    36961 cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac
    37021 gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca
    37081 agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat
    37141 ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg
    37201 cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt
    37261 cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata
    37321 gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga
    37381 aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc
    37441 gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc
    37501 accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat
    37561 ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata
    37621 tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg
    37681 tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag
    37741 ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt
    37801 atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag
    37861 gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa
    37921 gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga
    37981 tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt
    38041 tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa
    38101 acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac
    38161 tgattctttg cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc
    38221 tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt
    38281 tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt
    38341 atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc
    38401 atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt
    38461 atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga
    38521 aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat
    38581 tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg
    38641 atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa
    38701 acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt
    38761 ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta
    38821 tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg
    38881 tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt
    38941 tgaaagacac tgttctgatt ggactaggaa cgaatggctc attctcggaa gctcagtttg
    39001 atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga
    39061 cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca
    39121 atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg
    39181 atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct
    39241 tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac
    39301 aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt
    39361 ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt
    39421 tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag
    39481 aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag
    39541 caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat
    39601 aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc
    39661 tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact
    39721 ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt
    39781 ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc
    39841 tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt
    39901 agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg
    39961 attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga
    40021 ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg
    40081 atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg
    40141 tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa
    40201 aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca
    40261 cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg
    40321 ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat
    40381 tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa
    40441 ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga
    40501 gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg
    40561 aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc
    40621 tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt
    40681 cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg
    40741 atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt
    40801 aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc
    40861 tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc
    40921 agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt
    40981 gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc
    41041 ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga
    41101 tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga
    41161 atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct
    41221 gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag
    41281 tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc
    41341 gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta
    41401 ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga
    41461 gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg
    41521 gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc
    41581 agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca
    41641 cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct
    41701 tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag
    41761 caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa
    41821 gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc
    41881 gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca
    41941 ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc
    42001 ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg
    42061 acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata
    42121 acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat
    42181 tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg
    42241 caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag
    42301 cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt
    42361 gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa
    42421 gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt
    42481 ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg
    42541 atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt
    42601 tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca
    42661 ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc
    42721 gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca
    42781 gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa
    42841 cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct
    42901 caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat
    42961 aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga
    43021 ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat
    43081 cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc
    43141 gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa
    43201 gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt
    43261 gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta
    43321 gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg
    43381 caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg
    43441 tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag
    43501 tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa
    43561 aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa
    43621 gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat
    43681 caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag
    43741 gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag
    43801 caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa
    43861 ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa
    43921 caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt
    43981 ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta
    44041 aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct
    44101 gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat
    44161 cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct
    44221 gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa
    44281 aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg
    44341 gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa
    44401 ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga
    44461 gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag
    44521 atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa
    44581 gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat
    44641 gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag
    44701 ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa
    44761 gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca
    44821 gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa
    44881 gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata
    44941 tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt
    45001 tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca
    45061 ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt
    45121 caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa
    45181 tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc
    45241 tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt
    45301 attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt
    45361 gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg
    45421 gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt
    45481 agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt
    45541 agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa
    45601 tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg
    45661 ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa
    45721 ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat
    45781 tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa
    45841 aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt
    45901 gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg
    45961 gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca
    46021 gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca
    46081 tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact
    46141 gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa
    46201 atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta
    46261 caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc
    46321 ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat
    46381 caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga
    46441 gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg
    46501 ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact
    46561 tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta
    46621 ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc
    46681 cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa
    46741 gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact
    46801 gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa
    46861 gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat
    46921 gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc
    46981 ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg
    47041 cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc
    47101 gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat
    47161 gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa
    47221 caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc
    47281 catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact
    47341 tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca
    47401 cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg
    47461 ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg
    47521 aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct
    47581 gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc
    47641 gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg
    47701 gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga tctagctaat
    47761 tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc
    47821 agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt
    47881 gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca
    47941 ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg
    48001 attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa
    48061 acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac
    48121 ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg
    48181 atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt
    48241 attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct
    48301 gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc
    48361 ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa
    48421 ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg
    48481 atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg
    48541 tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc
    48601 ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc
    48661 atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa
    48721 gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc
    48781 gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc
    48841 gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc
    48901 cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct
    48961 ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc
    49021 gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat
    49081 tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag
    49141 ccagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat
    49201 tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag
    49261 tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt
    49321 ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata
    49381 agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc
    49441 gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat
    49501 cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa
    49561 cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat
    49621 caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac
    49681 aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt
    49741 cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta
    49801 aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga
    49861 ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt
    49921 ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt
    49981 ttatttagta a
//
LOCUS       DAGCXG010000010        49175 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGCXG010000010 DAGCXG010000000
VERSION     DAGCXG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.010"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..232)
                     /locus_tag="MB022_000581"
     CDS             complement(<1..232)
                     /locus_tag="MB022_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBT4465605.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFL"
     gene            complement(347..925)
                     /locus_tag="MB022_000582"
     CDS             complement(347..925)
                     /locus_tag="MB022_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4465606.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1002..1532)
                     /locus_tag="MB022_000583"
     CDS             complement(1002..1532)
                     /locus_tag="MB022_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBT4465607.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1536..2396)
                     /gene="rlmB"
                     /locus_tag="MB022_000584"
     CDS             complement(1536..2396)
                     /gene="rlmB"
                     /locus_tag="MB022_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBT4465608.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2389..2784)
                     /locus_tag="MB022_000585"
     CDS             complement(2389..2784)
                     /locus_tag="MB022_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBT4465609.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2781..4193)
                     /locus_tag="MB022_000586"
     CDS             complement(2781..4193)
                     /locus_tag="MB022_000586"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBT4465610.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4190..4726)
                     /gene="cysE"
                     /locus_tag="MB022_000587"
     CDS             complement(4190..4726)
                     /gene="cysE"
                     /locus_tag="MB022_000587"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBT4465611.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4812..5051)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5048..6508)
                     /locus_tag="MB022_000588"
     CDS             complement(5048..6508)
                     /locus_tag="MB022_000588"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBT4465612.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6678..7835)
                     /locus_tag="MB022_000589"
     CDS             complement(6678..7835)
                     /locus_tag="MB022_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBT4465613.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7869..9239)
                     /gene="radA"
                     /locus_tag="MB022_000590"
     CDS             complement(7869..9239)
                     /gene="radA"
                     /locus_tag="MB022_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBT4465614.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9302..9829)
                     /locus_tag="MB022_000591"
     CDS             complement(9302..9829)
                     /locus_tag="MB022_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBT4465615.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10059..10865
                     /gene="proB"
                     /locus_tag="MB022_000592"
     CDS             10059..10865
                     /gene="proB"
                     /locus_tag="MB022_000592"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBT4465616.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10862..12109
                     /locus_tag="MB022_000593"
     CDS             10862..12109
                     /locus_tag="MB022_000593"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBT4465617.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12185..12529
                     /locus_tag="MB022_000594"
     CDS             12185..12529
                     /locus_tag="MB022_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465618.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12646..12972)
                     /locus_tag="MB022_000595"
     CDS             complement(12646..12972)
                     /locus_tag="MB022_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465619.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13587..14789)
                     /locus_tag="MB022_000596"
     CDS             complement(13587..14789)
                     /locus_tag="MB022_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4465620.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14906..15421
                     /locus_tag="MB022_000597"
     CDS             14906..15421
                     /locus_tag="MB022_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4465621.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15620..16066)
                     /locus_tag="MB022_000598"
     CDS             complement(15620..16066)
                     /locus_tag="MB022_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4465622.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(16113..17591)
                     /gene="msr(C)"
                     /locus_tag="MB022_000599"
     CDS             complement(16113..17591)
                     /gene="msr(C)"
                     /locus_tag="MB022_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4465623.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17945..18574)
                     /locus_tag="MB022_000600"
     CDS             complement(17945..18574)
                     /locus_tag="MB022_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4465624.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18558..19022)
                     /locus_tag="MB022_000601"
     CDS             complement(18558..19022)
                     /locus_tag="MB022_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4465625.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19509..20777)
                     /locus_tag="MB022_000602"
     CDS             complement(19509..20777)
                     /locus_tag="MB022_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4465626.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(21008..21745)
                     /locus_tag="MB022_000603"
     CDS             complement(21008..21745)
                     /locus_tag="MB022_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4465627.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21812..22381)
                     /locus_tag="MB022_000604"
     CDS             complement(21812..22381)
                     /locus_tag="MB022_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBT4465628.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22509..23381
                     /locus_tag="MB022_000605"
     CDS             22509..23381
                     /locus_tag="MB022_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4465629.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23723..24595
                     /locus_tag="MB022_000606"
     CDS             23723..24595
                     /locus_tag="MB022_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4465630.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24592..26550
                     /locus_tag="MB022_000607"
     CDS             24592..26550
                     /locus_tag="MB022_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4465631.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26882..27733)
                     /locus_tag="MB022_000608"
     CDS             complement(26882..27733)
                     /locus_tag="MB022_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4465632.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27856..28323
                     /locus_tag="MB022_000609"
     CDS             27856..28323
                     /locus_tag="MB022_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBT4465633.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28586..29068)
                     /locus_tag="MB022_000610"
     CDS             complement(28586..29068)
                     /locus_tag="MB022_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBT4465634.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(29168..30031)
                     /locus_tag="MB022_000611"
     CDS             complement(29168..30031)
                     /locus_tag="MB022_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4465635.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30629..31504
                     /locus_tag="MB022_000612"
     CDS             30629..31504
                     /locus_tag="MB022_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465636.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31649..31801)
                     /locus_tag="MB022_000613"
                     /pseudo
     CDS             complement(31649..31801)
                     /locus_tag="MB022_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(32069..33400)
                     /locus_tag="MB022_000614"
     CDS             complement(32069..33400)
                     /locus_tag="MB022_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4465637.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLTTAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33549..34262)
                     /locus_tag="MB022_000615"
     CDS             complement(33549..34262)
                     /locus_tag="MB022_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4465638.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            34437..35861
                     /locus_tag="MB022_000616"
     CDS             34437..35861
                     /locus_tag="MB022_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4465639.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            36160..36867
                     /locus_tag="MB022_000617"
     CDS             36160..36867
                     /locus_tag="MB022_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4465640.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            36854..37177
                     /locus_tag="MB022_000618"
     CDS             36854..37177
                     /locus_tag="MB022_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4465641.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(37266..38303)
                     /locus_tag="MB022_000619"
     CDS             complement(37266..38303)
                     /locus_tag="MB022_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4465642.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(38624..39607)
                     /locus_tag="MB022_000620"
     CDS             complement(38624..39607)
                     /locus_tag="MB022_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBT4465643.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(40022..40792)
                     /locus_tag="MB022_000621"
     CDS             complement(40022..40792)
                     /locus_tag="MB022_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465644.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40785..41720)
                     /locus_tag="MB022_000622"
     CDS             complement(40785..41720)
                     /locus_tag="MB022_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465645.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(42036..43685)
                     /locus_tag="MB022_000623"
     CDS             complement(42036..43685)
                     /locus_tag="MB022_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4465646.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVSSKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(43802..46405)
                     /locus_tag="MB022_000624"
     CDS             complement(43802..46405)
                     /locus_tag="MB022_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBT4465647.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(46417..47466)
                     /locus_tag="MB022_000625"
     CDS             complement(46417..47466)
                     /locus_tag="MB022_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBT4465648.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(47492..47806)
                     /locus_tag="MB022_000626"
     CDS             complement(47492..47806)
                     /locus_tag="MB022_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBT4465649.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(47822..48268)
                     /locus_tag="MB022_000627"
     CDS             complement(47822..48268)
                     /locus_tag="MB022_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4465650.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(48269..>49175)
                     /locus_tag="MB022_000628"
     CDS             complement(48269..>49175)
                     /locus_tag="MB022_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4465651.1"
                     /translation="VKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHF
                     NEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVL
                     SINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFL
                     NLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFA
                     MPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIAS
                     NHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT        14439 a        10209 c         8575 g        15952 t
ORIGIN      
        1 ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt
       61 caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta
      121 aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc
      181 tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca
      241 cctctactat caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa
      301 gaaatgcaat aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat
      361 aaaatttcaa gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct
      421 tgtgatccat tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat
      481 gaaaaatttt agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta
      541 ataaaagatg ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc
      601 cttctgtctc tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg
      661 cgttctgctt cctcaacaga gaatagattt gattgcttat aacgctttta taataacttc
      721 caaagctaac acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg
      781 cttcttgttc ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta
      841 gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg
      901 cagcgcgcaa taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa
      961 tcttatcatc aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt
     1021 aaatcgtcca atttttggga caattttttt aactgctctt cattccatgg agaattacgt
     1081 cggaaatcaa taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt
     1141 atcgtcttat ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac
     1201 tgttctgcta aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca
     1261 gcgattctct cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac
     1321 tggtatttct tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg
     1381 acttccattc cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca
     1441 tccgccattt tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg
     1501 tagccgtcta caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa
     1561 gcctgataca taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc
     1621 atcgggatcg tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct
     1681 tcattaccaa tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg
     1741 gtcatatctg tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc
     1801 aaattcgtca cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg
     1861 ataggtgtga ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg
     1921 tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa
     1981 aagaaggggt cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat
     2041 tcataaggtg taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct
     2101 aatttttgtt taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt
     2161 tcttctactt tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt
     2221 tgtagcgctt ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca
     2281 ttttgatcag aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt
     2341 tttttgtctt caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt
     2401 ttcaccaact ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt
     2461 caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc
     2521 agcatttttt gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc
     2581 ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc
     2641 agaaacataa ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat
     2701 caggtggtca cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc
     2761 gttcaattgt gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct
     2821 tctaataaga tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca
     2881 cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac
     2941 tcttctgctg taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct
     3001 tgtgccaaga cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca
     3061 agctcataga ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg
     3121 aatcgctgtt ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct
     3181 gaggcagttt cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg
     3241 gtggctgctt ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc
     3301 gccatgaaga aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg
     3361 aaattcccta gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg
     3421 tgcatccagt aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt
     3481 tcatgatgag gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa
     3541 tgttttgttg ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt
     3601 gaatcccaag aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct
     3661 tctttttgct gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg
     3721 gattggtcac ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct
     3781 gaagattcat aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc
     3841 atatgatcca tgactcttgg atgcagtgta gcaggttcga cattcaacgc tttcgtatct
     3901 tcttcaaatg ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt
     3961 gctgtacgga tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag
     4021 cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg
     4081 atatggatat aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt
     4141 atagggatga attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc
     4201 tcctcacttt ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt
     4261 cgtctggaac atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg
     4321 ttaccggtcc taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt
     4381 gtcttttacc cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat
     4441 cgtcctcgat ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc
     4501 cagttccaat cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag
     4561 cattgatctt cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc
     4621 aaaacaaggc atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg
     4681 gatcgtttct ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct
     4741 ttcgtaagaa ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat
     4801 aagtttcctt atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt
     4861 atctctgaca gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt
     4921 tttcctgttt tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt
     4981 gataccttgc accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc
     5041 atcgtgttta gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt
     5101 cgatcgtttc aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca
     5161 tgaaaagatt ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga
     5221 cagttggagc atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag
     5281 taggaactgt ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat
     5341 tgaaaaatag cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca
     5401 ggctaacgat cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc
     5461 catcagcgat tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga
     5521 tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta
     5581 ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca
     5641 caggtgacca gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt
     5701 cgcgatattg ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg
     5761 tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt
     5821 catagatcat cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag
     5881 tgattttcat caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc
     5941 gtttttggat gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca
     6001 ccatatctgt aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta
     6061 gaccttgtgc ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt
     6121 aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg
     6181 tgcaatagca tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga
     6241 tttctttacg ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat
     6301 cccaattcat tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat
     6361 tacgtttttg gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa
     6421 acaaataatt gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg
     6481 gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac
     6541 taacagcaaa aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag
     6601 cacaaatttt accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag
     6661 ttggctgaat ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg
     6721 tcgattcctc gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga
     6781 agggcacttg ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg
     6841 acaaccatcg tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt
     6901 actactagaa cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac
     6961 gcattgatat taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca
     7021 ttggttacaa tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca
     7081 ctgatatctt caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag
     7141 gcatttagga tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc
     7201 gcgatgtatt gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat
     7261 ccagtctttg taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta
     7321 tatttatgga aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct
     7381 gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt
     7441 gtcgttccca ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca
     7501 ctattgacga atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc
     7561 aatccgatga tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt
     7621 ttttgttcta cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa
     7681 aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac
     7741 tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca
     7801 atgatcatca gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca
     7861 tttattagtt agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc
     7921 tcgattcctt ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg
     7981 aagccgagtt tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct
     8041 ccagtcagtc caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag
     8101 cttgaagcaa tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca
     8161 cctgcagctt ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc
     8221 actgccatga tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt
     8281 ccgaacatag taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc
     8341 attgccacaa cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt
     8401 gaaggattag cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc
     8461 gttgagccaa atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct
     8521 tcaaaatata acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct
     8581 tctttggtaa catgaccgac gataaaaatg gctatcccat ttgttttagc ggttttgagt
     8641 agttctgcag tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc
     8701 tgcgtcatcg tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca
     8761 cgactgattt cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg
     8821 aggcgttccg cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact
     8881 tttccgccta tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata
     8941 cctggatcgc caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg
     9001 ttcagttctt ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt
     9061 tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct
     9121 tgttcgattt cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac
     9181 ttaggcgaca catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt
     9241 gtaccgcctc cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt
     9301 tttatttact ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg
     9361 cttttaaaaa cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat
     9421 ccttgaaacc aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt
     9481 aataatcact gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat
     9541 tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag
     9601 taggcacgag ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat
     9661 ctgcgactgt ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct
     9721 cttctgctgc ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga
     9781 tattttgatc tttatattta gaaataactt caaatcctct ttttctcacc ggttttcctc
     9841 cctaaataaa ctactcctat tctaacatat ccgaggctgt aaaagacttt ctttacaaat
     9901 tcttcaagaa attctgctat cttaaagtaa accattaatt cgttcttttt ccaaacgact
     9961 gcaagttgta caaaaaaact attttttaca tgactttact cgtcaaaaca agtagaatag
    10021 aacgaaatta atgagtggat atttgattga ggtgtcacat gcgtaaagaa attaaaaatg
    10081 caaaacgaat tattgtcaaa gtaggaacaa gtacattgat ttatcccaat ggaaatatca
    10141 atcttgcggc aatcgatcag ttagcttttg tcctttctga tcttagaaat cggggaaagg
    10201 aagtgatctt agtctcatca ggagctgtag gcgttggtat gcataaatta catttgtcag
    10261 aacgtccgac tttgattcct gaacagcaag ctgcggcggc tgttggtcaa gccgaactga
    10321 tgaatatcta taatcaaaga ttcagtgtat ataatcaaca gacggcacag atcctcgtga
    10381 caagagacgt ggtagattat cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc
    10441 ttttgattat ggacacgatc cctatcgtga acgaaaacga tactgtagca gtggatgaat
    10501 tagatcatct gacaaaattt ggcgataatg atcagctgtc agccatcatg gcaaaacttg
    10561 tcgcggcaga ccttttgatc gtcctttctg atatcgacgg gttttattca gataatccga
    10621 ctacaaatcc cgaggctcag atgtatcgct atatcgataa aatcgatgaa aagattctgg
    10681 cacaagcagg tggtgctggt agcacatttg gaacaggcgg tatgcagagt aaattaaaag
    10741 ccgcttctag gatatttgaa gcaaacagcg caatgatttt agcaaatgga aaaaatccgg
    10801 caattatttt tgatattctt gcaggaaaag aagtaggtac tttatttaag gaggacttga
    10861 aatgactgac ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac aactagcact
    10921 tatgcctaca aaccgcaaaa atgagctact tgttcaaatg gcacaaacca tcaaaaagaa
    10981 tcaacaagcg atcattgaag caaatcataa agacttagaa aaagcagtcg aaaacaatat
    11041 cagtgaaacg atgcaagacc gcttgcagct gacacctgag cgaatcaatg ccatggctac
    11101 agagatcgaa aaaattgctt cgttagacga tcctattgga aaagtagatg agatgtggac
    11161 gaacaccgac ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat
    11221 ttacgaatct cgtccaaatg tcacgacaga tgcagctagt ctagcattca aatcagggaa
    11281 tgccgttatc cttcgcggtg gcaaagaagc attcttctcg aatcaattat tagtccaatt
    11341 attgcagcaa gtactggtca gtgctggcga atctccttat gccatccagt ttgtcgatga
    11401 tacttctcat gaaactgcag gacagttgat gcgattgaca gaatacttag atgtgctgat
    11461 tcctcgaggc ggagcaaaat taatccaacg agtcaaagaa caggcaacgg tacctatcat
    11521 tgaaacaggt acaggaaata atcacgtata tatcgataaa gaagcacagt tatctatggc
    11581 agtgaacatc atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg cggcagaaac
    11641 attgctgatc cattctgaga tcgcaccttt tttcttgcca gctattgaaa aagaactggt
    11701 agaacacggg gtctctttac gtgcggatgc tcgtgcgtta gaatatctag agacggctgc
    11761 tttagcagaa gacacagatt gggatactga atatctagat tatatccttg ctgtcaaagt
    11821 ggtcgattct ttggatgaag caattgcaca tatcaatcgg cataatacta aacattcaga
    11881 aacgatcgtg acagatagtt atgcggctag tcaacgtttc ttaaatgaag tggacgcggc
    11941 ggctgtctat gtcaatgcct ccactcgttt tacagacgga tcggtatttg gttttggtgc
    12001 agagattggt atctctactc aaaagttgca tgcccgtggt ccaatggggt taaatgaatt
    12061 aacttccaca aaatatatca tttatggcga tggacaaatc cgcgaataat gtttcgtgtg
    12121 aaacattatt cccgcctttt gccttttttc tgtaaaggtt ctatccaact agaaaggtgt
    12181 caatatgttt gaaattttaa tgattttttt tatttacctt atttctctga atatcgctgc
    12241 ttttctggga gtcggtattt tatccctttt ctttcaattc aaaaaacgaa gcatagggag
    12301 tcaacgggaa aaatggtcgc agtattttga caaaatcggg caaaaagggc tggttacacg
    12361 cttgcacatt agttacatgg ttgcattatg cttattagcg acaatcaatt actattcttt
    12421 tttcgatcat tcaatcgctt atacgattac gctactgatt gctgggattt ttcacttatc
    12481 ttataaatat cagctgaata aaaaccactt gaatcggact ttccgctaaa aaatatccgt
    12541 acgataagta gagtgccttg aagatcggca aaaaactgcc ggacttttta gaggctgtga
    12601 caaagatttt gtcacagcct ctttttccat ataaacggcg ttcgcctacc agctgcctgg
    12661 taaatctgtt tcctcttcgt taagaagcga aaatccgtca aatgtttcgc catcataata
    12721 acgaagcagc tcatccatgg acaagttcat cgttttttct ttttcttctt ccgctgattc
    12781 agccagtagc tgttcccgat acacctgata cataccagct attttttgct gcatcgcttt
    12841 tctagtttca ccttccgcat tgattattgg caattctttg atatagccac gataaatcaa
    12901 ccgatcatct gaaaattcaa tgggtaattc aacgagtgtc aacccgactt cctgaaaata
    12961 ttcttccagc atctattatc cccccattct ttttcttaaa tgaattttag cttacctact
    13021 gaaaattttc cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac
    13081 atcccctttt tccgaataaa tggtgttctt ctgccaactc ctcaatcata agtcacaata
    13141 ttcaacaaac atgagaaact gttttttgaa attgttcctt gtgattcaga gttaaacgac
    13201 agtctcacaa cctcttggta aacggcgttc aatcagctga ccctcggtat tagctcaaaa
    13261 aacggtaaaa tatgagaagc tatttttccg ttttgtttct ctaatactta ggataaaacg
    13321 ctgattttac aacctctgtt aaacggtgtt caatcagctg tccttcggta ttagctcaaa
    13381 aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca ggacaaaacg
    13441 ctgattttac aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    13501 aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg
    13561 acagcttttt cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg
    13621 tgataaatga tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag
    13681 ctttcaccaa tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa
    13741 aagcggacaa aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata
    13801 gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg
    13861 atcggtacaa ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat
    13921 aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca
    13981 acgaaaatcg aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct
    14041 aattcagaat agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa
    14101 ttatataaca atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca
    14161 ccttcccaaa ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg
    14221 gctataaaac cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca
    14281 ctgcccaaga ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca
    14341 tacatcatga ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca
    14401 gtagagacaa ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg
    14461 atattcccag ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc
    14521 aatgtttttt tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca
    14581 gtgactaaca tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt
    14641 gcagcaatcg tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag
    14701 gtagctgcta caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt
    14761 atatctattt gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt
    14821 tattttacat gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga
    14881 aaataagacg agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct
    14941 tttatccaaa ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc
    15001 ccatcacagt cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt
    15061 tttggacaaa gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc
    15121 tcttgtagag gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct
    15181 agcaaaaatc tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac
    15241 tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc
    15301 gctagacaag aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg
    15361 tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta
    15421 agggataaac agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag
    15481 agcaatacag ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga
    15541 tcactatttt tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt
    15601 ttcaaataac agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa
    15661 gcgggccaac gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat
    15721 tcatataagc agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag
    15781 acatacatgt caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct
    15841 tgctttctgg atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga
    15901 agacatccaa aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata
    15961 aatgaagatc ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac
    16021 acatcattgt tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct
    16081 ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt
    16141 attttctgcc cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca
    16201 tgagaagtaa acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc
    16261 gttgccatat caataaaatt agtcggttca tctaacagca acacattact tggcttagta
    16321 aaaagcaaag caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc
    16381 gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa
    16441 aactctgttt gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc
    16501 ttttgctgat atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg
    16561 cggatatgtt ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt
    16621 ttacctaaag aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat
    16681 tggacatttt gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga
    16741 aaacggattt gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc
    16801 tctaatgttt tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa
    16861 gacaaccgat ccagtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt
    16921 tccgcttgtt ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc
    16981 gactctgctt ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta
    17041 taattccccg aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc
    17101 tcatctagaa aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc
    17161 tcttcaatca gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc
    17221 atcccttgtt cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg
    17281 ctccatttcc gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa
    17341 tttaggtcct ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct
    17401 gtttggattt tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat
    17461 ttaccgacac cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa
    17521 tcaatagata gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct
    17581 aaattttcca tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa
    17641 aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa
    17701 ataactatga ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa
    17761 aaaatggaca tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag
    17821 ttgttattca aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt
    17881 tttattaggt taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa
    17941 ccttttattc gaacatagtg atcttttctg gtgctaaacg aacagactcg taaccttctt
    18001 ctaccgcttt tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa
    18061 atgcctctgc caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat
    18121 cacgcgcaac taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt
    18181 tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc
    18241 tgacttcttt tggcatcttc cctgattcgt aagcttggtt gtagatttct tcgccatatt
    18301 cgtaacattg catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg
    18361 tattgaaacg gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa
    18421 agcgccatgg tttcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca
    18481 gcatttcttc gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag
    18541 tcataattgt tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt
    18601 gttatattgc aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct
    18661 acatcgctga aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc
    18721 ttgattttct tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga
    18781 tttctctgac gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct
    18841 ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc
    18901 agtctcatca tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg
    18961 acgatttttt gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac
    19021 ataatttcct cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa
    19081 aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt
    19141 atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct
    19201 aaaaccaaga ttattgattc ttatcaaact atagaagata gctcttctgc agtagataat
    19261 tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa
    19321 ttggatcagt atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt
    19381 tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa
    19441 catgaaaatc tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact
    19501 acctcttttt aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc
    19561 caatctcttt ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta
    19621 aatagtcgat aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa
    19681 ccgatttttg ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg
    19741 taaggattgt ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct
    19801 tcatattccc agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa
    19861 tgttctcctg tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta
    19921 tcatactgtg tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc
    19981 gcagtggcat aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt
    20041 ttccatgcac cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt
    20101 acgttatctt gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta
    20161 atccattgcc cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt
    20221 cctttaatcc caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca
    20281 ctttcaacga tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa
    20341 ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga
    20401 gcataaaaag catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt
    20461 gtcaatgttt gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc
    20521 tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt
    20581 tcttcggttg aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg
    20641 ttttcaattc ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct
    20701 tcagctagca ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat
    20761 aaattctttg ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt
    20821 gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta
    20881 ataaatttgc tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc
    20941 cttaaattga tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg
    21001 ttatggatta tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt
    21061 ctggcgtttc tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt
    21121 ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt
    21181 cagtttgaac agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt
    21241 gacgtaatcc ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat
    21301 caggataaac aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt
    21361 tttccatgat tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa
    21421 gcatattttg ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg
    21481 cattattgta caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt
    21541 ggacttgtgc aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag
    21601 attcttttat tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt
    21661 tttctgctaa tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa
    21721 tcactttttc tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt
    21781 atactcacta tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga
    21841 ttttggcagg gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat
    21901 tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc
    21961 cgatccaagc atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg
    22021 gatcggttag atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt
    22081 ctaaacagat gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc
    22141 ctatactaat atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat
    22201 ctactttgtc actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc
    22261 cagtattcag cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa
    22321 agagcggatc cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca
    22381 ttttgctccc tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat
    22441 aaaacatact taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga
    22501 gggaaaaaat ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata
    22561 tcagccgagc agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag
    22621 atttagaaga agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga
    22681 cggattctgg tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa
    22741 cggcgaacaa tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg
    22801 aaactaaagc aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg
    22861 atatccagtt tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg
    22921 gattacttga ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc
    22981 gactgccagc ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc
    23041 aaacagtcat ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt
    23101 cagtggacag tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg
    23161 gaacatataa cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat
    23221 tagctctaga tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc
    23281 cgccattaca agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg
    23341 ctcgtaaatt tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg
    23401 ctttcagctt tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag
    23461 tttcttaatt tgttccttcc ttttatttct gataattcca gtaaattact ataattataa
    23521 aaaaagaatt gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt
    23581 ttcatgtgac tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga
    23641 ggtttttcgt gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca
    23701 aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt
    23761 ggtagatgac ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa
    23821 aagaaatgat ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg
    23881 tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga
    23941 aacgatcatt gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat
    24001 cgctttgacc gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg
    24061 caacaaactt ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg
    24121 ggcagtagag tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata
    24181 tatcgcaatc catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg
    24241 tgaagtgacg attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat
    24301 ccattcagaa ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct
    24361 acaacgaacc catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa
    24421 acgaaaatac ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga
    24481 ataccagctc gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt
    24541 aagaagcgca atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca
    24601 tatctacaac gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct
    24661 ttactagtag ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt
    24721 ttacaagctg gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta
    24781 gcactaggga tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt
    24841 gaagttccag taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca
    24901 gaatcagttg atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga
    24961 ttgattgcgg cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct
    25021 ttctttagcg gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt
    25081 gcagtgctac tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc
    25141 attttgagtt taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc
    25201 cctacaggat tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat
    25261 gatattggta aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct
    25321 ggatttttcc caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat
    25381 gcacgtccag aaaacaaagc aaaaacagct tccttgatgt tagcagcagc ttttgcttca
    25441 ttctttactg gtgtaacaga accacttgaa ttctcattta tgttcgttgc ttggccatta
    25501 tatgtcttgc atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg
    25561 acagcaggat ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg
    25621 attgcgaatc agccattgat gttgatcgtc caaggattag taacagcagt gatttactac
    25681 tttggcttta actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca
    25741 gatctttcag atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca
    25801 agcaaaatct acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact
    25861 acacgccttc gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc
    25921 actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca
    25981 gaagtacaat tcgttgcaga tgagatggca aaactacatg gtggtgctgc aacacgacct
    26041 gcacaaacaa acaccgtggt taaaacagaa acatttgccg gtggaacgat cgaacaagat
    26101 atttacgcta tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc
    26161 gcggaaaaaa tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct
    26221 ccaatcgctg gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca
    26281 gatgctggta tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa
    26341 ccattcactt tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta
    26401 gttgatcttg ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca
    26461 aatggcgaca aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat
    26521 aaaatcggct ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga
    26581 taaacaaaca ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg
    26641 cgttctcctg ccaactccgc ggtaataagt ccagccaaac gtaacatatg aagagctatt
    26701 ttttatttga ctcttcttat tacttgatgc agaacagctt ttttacaacc tctgataaac
    26761 ggtgttcaaa aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca
    26821 ttttcacaaa atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac
    26881 attattcctg atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca
    26941 atactttttc taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga
    27001 tcgctaaaaa catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa
    27061 acgctgcatc acttgcttca ataatttcta actttttacc tgtcgcacgc tctaatgcct
    27121 tggctaattc ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac
    27181 tttctactag tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt
    27241 ttccatttcc tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta
    27301 attcattctc tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa
    27361 tcattttttc agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg
    27421 caaaacttgt ataagcgata taagataccc cagctgcttg tgctgccttc actacattct
    27481 tatgttcggc ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt
    27541 tcaacgcttc tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag
    27601 cttctttcag tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg
    27661 gaaccatctt ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta
    27721 ctaaataatg catatttgcc actccctatt ctttttattt gttaagattt acagctgtac
    27781 atttataaat tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg
    27841 aaaggatgac aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc
    27901 tatattgaaa tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc
    27961 gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc
    28021 tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta
    28081 ctagatgtct atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca
    28141 aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg
    28201 ctgcagaaaa aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa
    28261 atagccgttt tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta
    28321 taattctctc tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat
    28381 tctccggggg acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc
    28441 gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct
    28501 gaaaaatgca aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag
    28561 cgctactttc acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc
    28621 ccatatattt cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg
    28681 cgtaatcttc tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta
    28741 attcttcagg cgattctgct aaagcgatta caacaagata cgttcctttt gggaatggga
    28801 tgattcgggt agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt
    28861 tcccgttata gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg
    28921 catctattgt tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac
    28981 cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg
    29041 gtttttcttc gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat
    29101 cttacataaa attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat
    29161 tttttaacta tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat
    29221 cctctcccca ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag
    29281 gaagccattc tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt
    29341 tattaggaaa aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg
    29401 gattttcttt tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc
    29461 gggctccatc aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact
    29521 tatgcatctc ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat
    29581 tgactccttc aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt
    29641 tcttttcgat acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc
    29701 gtggaaaaga tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat
    29761 cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg
    29821 tcactcttga tccatctagg agatcgcgat tagccaaagc caatctacgg tttttgatat
    29881 agtccattag cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga
    29941 ttcctgtatt ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt
    30001 aatcgatcaa ttggttcaga tctttaagca tatttatctt cctccttgct tgccttttta
    30061 tcatattatc aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag
    30121 aaaatgttgt atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta
    30181 ttatctgcct atctatgttt tacctttaga aaaataaatt ttggggaaga cacattctaa
    30241 gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt
    30301 acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat
    30361 acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt
    30421 ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt
    30481 tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt
    30541 gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata
    30601 aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa
    30661 cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac
    30721 aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg
    30781 ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa
    30841 acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc
    30901 actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt
    30961 aacagagatt cttatagaac atccgcaatt tatcgaacgc ttcaaagtta agatgccatt
    31021 tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg atcatattat
    31081 caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca
    31141 agaagcagtt gaatcgctca ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa
    31201 tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct
    31261 tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga
    31321 aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc
    31381 aaaacgctac tccataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca
    31441 aaagaaaaaa atagataaaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa
    31501 atagcaagta aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt
    31561 aaaaacacga atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt
    31621 catttgacta ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg
    31681 ggtagatttt ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag
    31741 gttccactgc atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc
    31801 actctaatct ttaaagataa atattaaaat aacgattaat aatattaaca gtctttattt
    31861 ttcaccacca aaaacctatc tttgcatata ctaaatagac gtatatacac agataggttt
    31921 gtcttgtgaa aaataatgat tatccttacc aaaaaatgtt tgtttttcta catgttcgta
    31981 cttatcaaaa aatatgatcc ttattgaaat tactggctat ttatattcca acgacttctt
    32041 gtgttaattt ttgttatctc atagtaaatt atgtttcatt ttcacttaat ttagttgtca
    32101 cgtgttcgtc aattcgtaaa aacggtaaat atataaacac acttgcggct ataattaata
    32161 tttgtaaaac agctgctcgc cagtctccgg ctgttgttaa aaatcccgat ataactggtg
    32221 gcgtagtcca aggaatttgc actactacat gattaattaa tcccacagca gttgcaaaat
    32281 atgctactgt caaagaaaaa atcggtgcaa gcacaaaggg aataatcaga ctgatattaa
    32341 atacaatagg caatccaaaa ataataggtt cgcttatatt gaatatagcg ggagttaccg
    32401 ataatttagc aaagtcctta taatcttttc tacgactgaa gataaatatt gcaatcaata
    32461 aagcaatcgt atttcccgat ccacccatta ccgcaaaggt atctttaaag gccatattga
    32521 taatatgtgg aatttgctct ttgtttgcat aagctgccat attttcttgc atattctgaa
    32581 tcaaaaacgg atctaacaat gaacctgata ttactgcttg atggatacca aatccaaaaa
    32641 atagattagc aatagatgta attaacaaaa atcctggtaa accagttgta attttactta
    32701 atggctctgt aactatcgtg gtaattagct tatttatatc catattaaac aattgaatga
    32761 ttataaatga caataaagca aacgaggtaa tcgtaataat gattgggatt aatacattaa
    32821 aagactttaa aacagcggga ggaatatttc cggatagctg aatctgtagt ttcttattat
    32881 tagaaagttt aataaataat tctgtagccg ctaatccaac aaatatacct acaaacattc
    32941 ctgaagctcc tgtataggat ataggtatca cacccgtcac ttcaacagct ttatccgcat
    33001 tttcaggagt aaacatcatc tgcataggtg ttaatacaac aaatgtcgaa atagcaacta
    33061 ataccgctgc aatatgattt gtataatttc tgcttgcaca catatgataa gatactgctg
    33121 ctacaactaa taatgtagtt atgcttaatg taccattggc aattgaagta ccaatttgtt
    33181 gtaacgtggt caatgtacta gtatcgacaa tttcccccat gaatcctgtt ggttctaaga
    33241 tcactgaatt aataagtgtc acaaatcctg ctaacatcat aaatggcata aatgtagcaa
    33301 acgcatctcg caaagttctt aaatggatct gattccccaa cttagtcgca actataccaa
    33361 atttttcaaa tacaatgtcc ttcctgctac ttttgcccat attttttctc ctttatgctt
    33421 attgtaatcg attacttttt ccacccaaat aatacatcac ttatcaaaaa aagtaaatac
    33481 ttttaattaa atatatatat cttttttagt ataaaaaaat aagctctgaa aaaataattt
    33541 tcccgagctt atttttcaac aaaaacagct tcaaaaacaa agtcttgata taaatgtctc
    33601 gataccgaat actcgaaagg tttaccagac gatagagcaa caactttgtc tatctcaatc
    33661 ataaattcac ctggatctat ttttaatatt tttgcgtcaa attcattcgc agtatctcct
    33721 ttgatacgta tgtgcgaagt ctttatttcc agacccagtt catttgtgat ataagcatat
    33781 acagatttct ttaaatgttt tggttccaag cctggtatga ctgctaatgg catgaatgtt
    33841 tgctcaatcg agtatggttg acaatcaata aaacggcttc gtacaatatt atatacatag
    33901 tcttttacac tgatacttaa tttatcagct acttttttcg atggaagttc aattgagaaa
    33961 gtatggattt ctgaatctac gtgttcttca ccaaccacgc taaacaaacc ttcgtttgga
    34021 ccaagaactc gtgagttatc aattggtgtt ctgacgactt cagtcccata cccacttcta
    34081 cttcgcaaaa ctccttcgga aacaagtaag tctacgcctt tctttactgt taacttgctt
    34141 gtatgataaa taactgccaa ttcatctcct gtaggaagtt tctcaccaat tttatactct
    34201 ccatttacaa tttttttctt tatatctaag taaatttcca tatacttcgc aatttttttc
    34261 aatggtgacc ctccaatcaa aaacatatct tcatctatac tttaacataa ataaaccgat
    34321 aaaatccaca tcaaaattat acacacaata aaaaagatat atatatttaa acaaaactat
    34381 ttactttaag ttgtaagcgg tgttatcatt aagtggaaac aggaggtaaa aaaattatga
    34441 caactatgaa aaagctaact tttccagatg atttttggtg gggttccgct tggtcagctg
    34501 agcaagcaga aggaaaagga gaaacaggaa aagcagagac tatctgggat cgctggttta
    34561 aagaacaacc ttaccggttt tatcaacgaa tcgattcctc tattacaacc gatcacatcc
    34621 atcaatataa agaagatata gaactaatga aaaaaactgg gcataactcc tttcgcgttt
    34681 ctatttcttg ggcacgaatg tttccaaatg gcggtaccgg aaaagtaaat cccaaagcca
    34741 tttctttcta tcgaaaatta ttttccgaaa tgaataataa cggaatcaaa gtatttgcca
    34801 acttatatca ttttgacatg cctgctactc ttcaagataa aggaggatgg gaatcacgtg
    34861 aagtagttga tgcttatgtt cattacgctg aaacttgttt taaagaattt ggtgacttag
    34921 tttgtcattg gtttaccttc aatgaaccac ttgggcctat tttaggtact tatcttgaag
    34981 attttcatta tccaaacctc attgacttta aacgcggagc gcaagcagca ttcttcacta
    35041 tacttgcaca cgcaaaagca atacaagtat ttaaagaact agctcttcct agtaaaatag
    35101 gcgttattct aaatttgagt cctacttttc ctcgtagtca aaatcctgct gatgtgaaag
    35161 cagcagaaat cgctgattta ttttatacgc ggagcttttt agatcctatg gttaaaggga
    35221 atttttctaa aaagcttatc ctgttattac aagaatataa tcagcttcct gaagattata
    35281 cgaaagaaga tcttgtatgt atctacgaaa ataccgctca aattcttgga ctaaactatt
    35341 acgagccacg tcgcgtcaaa tctcgttctt ctatggtgga tacatctagt ccatttttac
    35401 cagagtggtt tttcgaacca catatcatgc ctggacgacg aatgaacgaa taccggggat
    35461 gggaaattta cgaaaaagga atttatgatt tgtgcatgga tatcaagaac cattatggaa
    35521 acatcgaagc atttatctcg gagaacggaa tgggcgttgc ggatgaagaa cgtttcatgg
    35581 atgatacagg gcaaataatt gatgattatc ggattgagta tatcaaagat catcttgctt
    35641 acttatggaa ggcaataaat gaaggatgta acattaaggg gtatcatctt tggacattta
    35701 ttgattgctg gtcatggatc aacgcctata aaaaccgata tgggctggtt tcactcgacc
    35761 ttactactca aaaaagaaca attaaaaaaa gtggcgaatt ttacaagcaa ttaagtgata
    35821 caaatggatt tagatacgat gtagacttgt tagttgaata atattctaaa attccacttg
    35881 aagtgaacac attagaaaac aagaataatg agaaatacca ataacaaata ctttttcttc
    35941 atacttcatt tatacttgtc agtataagag actgtgacaa atgtagtgat cctcagaagt
    36001 taggtctgga aatctaactt gagaagattt ttttaaacta cgtccagtct tttttatttg
    36061 tttaaaattg aagttgacaa gtcttcatta aaaatttaga atattcacaa gtttgacgac
    36121 aattatgaaa atttatgtcg tgctaaagga gttttgttat tggatgaaaa gttagatata
    36181 aaaactgcga tcaaagacac gttacctacc gtttttggtt atatcggtat tggacttgca
    36241 tttggtatcg ttgggaaagc tgccgggttt catccgctgg tcgtcacgtt gatgtccctg
    36301 ctgatctatg ctggttctgc ccaatttatc acggtcagca tgcttgctag ccatagtcca
    36361 ctgctctcca tcgttctctc gacctttcta gtcaattccc gaatgattct gatgagtatg
    36421 acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa atctgctgat tggcacattg
    36481 ctgacagatg aaagtttcgc actgggaatg aataaattga actatactgg acaaaagtta
    36541 aactttcgtt ggatgaatac agctaattgg atttcttatt tgacgtgggt gggttcttct
    36601 cttgttggcg cacttttagg gaattttatt actgatccga aaaagtttgg tttggatttc
    36661 gccatcgtcg ctatgtttat cgggttactt tatcttcaag tcatttctga tagaaacacc
    36721 tctaaacgat tacagttgat cctgattggt ctcactttga ttctggtata tgtcggactg
    36781 atttttatcc caagtaactt agtgatttta gttgtaacac tgattgggtg cggattgggg
    36841 gtgtggatca aacatgcctt cttttgagtt tacattgctg actattttag gttgtacttt
    36901 agcaacttgg ctttctagga tcctaccatt tgtcttactg aaaaaattcg atttgccgca
    36961 accgttgctt gagtatttaa gttttgtccc gatcgtcatt atgtctgctt tatggttcag
    37021 cagtctattt acccaaaata tcggacatct gccgcagatc aatctcgaaa atgctttggc
    37081 ttcagttcct acacttttag ctgctattct ttcaaaaagc ttattggtga tcgtcttggt
    37141 aggaattctt tccctttcgt tgattcgctt attataaata aaaaaacaag agcttatggc
    37201 aaattactgt catgcgctct tgtttttatc aaagtcaaat aagaaaaaaa tcggcgtatt
    37261 cttgcttatc cgttgaacgg accactaaca acgacttttc ctaatatatg tcctgtttct
    37321 acgtctttgg ttgcttgttt caagttcttc gcattcatcc catctgaata gaccttgctc
    37381 aatgtactgc ggataatccc ttcatttgct aaagcagcaa tgtgcgctaa agcttctcct
    37441 tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca tatactccca gtcgaagctg
    37501 atagattggt ttttccaaac acctatatct aacggctctt tgatccctac gatcgttccc
    37561 acatgtccaa atggtcggac aaggtgtttg atttgatcaa agtagcgggt aatatcgaat
    37621 aatacagcga catactctac ttgatcgatt cctaattcac gaaaactttt acttaagtcg
    37681 tgatgataat cgattggata gtctacacct gtttttttca gccattcaaa attcttcgga
    37741 cttgcagtcg cgtagacttc taggccagcc caatgcgcta gttgattcaa aatcgaaccg
    37801 acgcccccag ctccgttgat cactaagatt tttttcccgc ggttggcatt ttcttctgga
    37861 gtcagaccaa atttttcaaa taacaattca taagcggtca atgaggtcaa cggcaaggct
    37921 gcggcttctt catctgaaag attttttggt gcaagagcga caatacgttc gtcaaccagt
    37981 tggtattctt gattgcttcc tgctcttgtc gttgtacctg cgtaaaaaac gcgatcgccg
    38041 acagaaaatt tttgcacttc atcacctaca gcaacaactt tcccgactcc atcaaaccct
    38101 aagatacgta aagcatttct tatcccgtta tcttggcgaa gtttcgtatc gacaggatta
    38161 acagatactg cgttgacttt taccagaaga tcccgtgaac tgggtacagg tatctgttct
    38221 ttttcatcaa taaagctttt tggatcatcg atcgacaacc cttcataaaa accgactacc
    38281 ttcattaatt ccttttctga catacgaatt cctccttagc ttcatcaagt tggagcatcc
    38341 atacagtttt catttcaata tatatagtat gctatttgtt ccacttttat tcatcataat
    38401 tattctctta cttaaagtaa actatttgca cttttttggt taaaaaagag gttgcgacaa
    38461 gacttttgtc acaacctctt ttaaacggtg ttcaatcaac tgaccctcgg tattcgctca
    38521 aaaaacgaca aaatatgagg agctattttt gtttggctgt tcttattact tgatgcagga
    38581 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
    38641 gcgttggatg ttctttttca aaggtgtcta agatctctcg tacttcatca gtagatttcg
    38701 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
    38761 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
    38821 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc ggttcttttt
    38881 caaatgcata aggattcttg aaaatccccc ggccaatcat gatgccatct acgccatatt
    38941 tttcggccaa ttcttctcct gtctggcgat ctgggatatc tccgttgatc gtgatcagtg
    39001 tttgcggagc gatttggtca cgtaaagcaa tgattttagg gatgacttcc caatgtgcat
    39061 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
    39121 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
    39181 tgacgctgac aggcaaaccg ccagctttag ccgcttcaat caattgtgcc gctacttctg
    39241 ggcgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
    39301 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
    39361 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaaggtca
    39421 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
    39481 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
    39541 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
    39601 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
    39661 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
    39721 ctgattgagc aaaaaaagac tgtgacaaaa atcttatcac aattcctttt ccggaataaa
    39781 tggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aaacgaaaaa taggaggagc
    39841 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca acctctccac
    39901 taaacggtgt tcaatcagct gaccctcggt attagctcaa caaacgacaa aatatgagga
    39961 actattttcc cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
    40021 tctaacttct caatcctctt cctttattga tcaaatacca agtaacggcg tacaaaacaa
    40081 cgatgaatag cacaagtacg atcatcgatg ttgctactgg cacatcgatt gttccaagga
    40141 atccgtaacg gaaaccggag atcatataaa cgatcggatt gactttcgaa acagcttgcc
    40201 aaaatggcgg caacatagag atggcataga aaacgccacc tagataagtt aacggctgca
    40261 agacaaatgt aggcacgatc gatacatcgt cataagactg agcaaaaatc ccgttgagca
    40321 gtcctgctaa cgaaaacaag attgccgtca tcaataatgt aatgatgacc atgaaccatg
    40381 aatacacatg aagtggtaca aagaacaagg aaatgatcgt tacgagagag ccgacgagaa
    40441 tactgcgacc cactccgcca atcacaaagc cccaaatgat cacatgcgta ggaacaggcg
    40501 ctaccaagat ttcttcgatg tttttctgga atttttgaga gaagaaggac gaagaaacgt
    40561 tggcatacga actagtgata gcagacatca taatcaaacc aggcacgatg aattccatat
    40621 atgaaaaacc acccatgtca ccgatccgac caccgatcat ttttccgaaa ataacaaagt
    40681 acaaggatgt cgtgatcaca ggcggaacca gtgtttggac ccagatacgc aaatagcggt
    40741 tcgtttcctt gatagctaaa ctttttaatg cagtaaaata aagattaaac atttttctcc
    40801 tccgtatgat tattttcttc tgtgattttc aaaaacagtt cttctaaacg attcgactta
    40861 ttcctcatag aaagaacttt gattccttgt cttgtcagct gatcgaagat tccgttcacc
    40921 ccttgatttc gttcaacttc gacagcgatt gtctgttcat cttcaaatgt acaagcgtat
    40981 ccttcaatca caggtttggt ctcatatggc gctaaatcaa agataaatgt ttcaaattgc
    41041 agtttggata gaagctcttt catactagta ttctcgatca gttcacctga ttggatgatc
    41101 ccgatgttgc gacaaagcat ttcagcttct tctaaataat gcgtcgtcaa aatgatcgtt
    41161 gtaccatttt cgttcaattc tcggagaaac tcccacatct cgcgtctcaa ttcaatatcg
    41221 actcctgctg ttggttcatc caagatcagc aaacgtggtt catgcatcaa tgccctagca
    41281 atcatcagtc ttcgcttcat tcccccggaa agcatacgtg cacggacatt tctcttttcc
    41341 caaagattgg actgcttcaa atatttttcg ctgcgtttca tcgcttcttt gcgagagaca
    41401 ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg tttcaaaggg attgaaattg
    41461 aactcttgcg ggactaatcc aatctgttgc tttgctcgga ctagatccgt atccagatca
    41521 taaccgaaga cattcacttt tccagatgtt ttattgacta aagaagtaat gattccgatc
    41581 gttgtagact tcccagctcc atttggtcct aatagcgcat agaaatctcc ttctttcacg
    41641 gaaagatcga ttcctcgcaa tgcttcgacc ccacttccat aaaccttttt caaattattt
    41701 atttcaagcg catttgccat ttatttccta tctccttata gacatgattc tttattgtat
    41761 catggaatac agcagatgaa aaaacaactt ggttccagcg aaaaatcagg ttgctgggct
    41821 caggaaaatc tcctaatgaa agacatcaat cggacgaagt cagacaacct tttcagaaaa
    41881 aacaaatcga agataaagga ttactattca tatgtgcaac aaaaggagcg tgagacaaaa
    41941 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc aaaagccaag
    42001 atttttgatc caataaatac tttcgatcac ttcgcctatc ccaatacatg ccactgccgc
    42061 ttttcatcaa ataacgcggc ttccgttttt gcggtttcta ctaaagtctc tgggtaatcc
    42121 aaaactttca gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa
    42181 tcaaaactga ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa
    42241 ttctttaaaa tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac
    42301 tccgcaagcc accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgtccct
    42361 tttaaaatct catctacgaa gcagtagcag cgttctttgg aagacacttg gtccaacaaa
    42421 cgtttgatcg atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg
    42481 attgccatgg aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt
    42541 tggatcgtct gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct
    42601 gatgcattgg aacccgtgac taatgtattc ttctgccagt tgaccggatt cactaccgct
    42661 tcttttaaca atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt
    42721 cggcaagtga tgggcatata agtacggtaa ttcaatattg cagcggctac ttctaattct
    42781 cccagcaatt cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg
    42841 aaatgatagg agataaatgg cagcatgaac atgatgttga catattcaaa aatcaactct
    42901 gcttcactcc cactcttcat acgaaatgaa aaacctaatt tagggatgtc tcttaatggt
    42961 ttagtttgct gtttcagctc ttcttgaagt ggtgtcggca gttttgctag ctgattggcg
    43021 gctgaaatcg ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc
    43081 ttcactagat aatagaccgt attgaatact aacccaccga ttgccaaata gattcctagt
    43141 acttgtccta aaagaagcaa acccaaaccc accagcggaa gcaagccaag aaaaataaat
    43201 aaacctacgc gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta
    43261 tgatcttttt ttcctaaacg agcgaattga tattggattt tttctctcag ttgaggattt
    43321 tcctgataaa aagcaatcag tttttctaac tgctgatctt caccaaaatc aaagctgcgt
    43381 aaacgttgat acaacgcttc tgaacctaca ctagaatagg tactgttgat tccttcgaat
    43441 acttcgaaca tatccaggtc ataccaagta agatcatcga tttcgctatc ccatgaacgg
    43501 aacttcttct ccgtgagcca tgcttccttc aagctttctt ctttatcaaa tctcggttga
    43561 taaggaattt tttcccattt gctacgaact gtttccttta atttcaaacg tgaaaaaatg
    43621 tctaaagcaa ctaataaaat aacagctgca ataataatta aaacgataaa ttctctttga
    43681 cccatatccc tctccgttcc tttatttgtt agctttagta tagcactgtt gcgctatttg
    43741 aaaagagggt aaaaaaacac ctttcaaaat aaaaggtgtt tttttcttgt atattacata
    43801 tttatctctc atttatcaaa attgacaaat aatcataggg aggaattgtt gttaattctt
    43861 gtttattctc cagtgcattg acaactgtcc caagggaaag gatagcctca ggtactattt
    43921 tttcttgttc tgttggatta gctaagcgaa gaacaaatcc ttgttcatga aaataagaag
    43981 gataaatcga tgatacaatt aagtcttcag gtaattcaat caatgaaaat ttacgaggaa
    44041 catcaagttc attttgtaat ggccaaatct tgttatctaa cctgtgaata aatacattca
    44101 gtttctgctt ttgataagaa atagattgtt ctaaccgttt ttctgcctgt tttgccactt
    44161 tgaactcatt aaatcttcct tcttctacat taaatccaaa agtgacttta tactcaccta
    44221 tctcttgagc tagcggtgtt tccatcatga catgcccttc atttgtcgta tctcctgaag
    44281 ctcgcccagg tcgccaagca agattaggtt ttccaagttc ccctgttgtt gaaaataagg
    44341 ttatgattaa ttgattttct atacgttcat actcttttaa accatctgca taaaacgtaa
    44401 aataatgatc attttctgcc acagagacac tcttatcgaa tatttctaaa ttaacaggtt
    44461 tctctaccat agtttctgac caattcggag gaatgacatc aggggtattt tcaataaatc
    44521 cgttttgaat ttgtgcaatt gatttttcag atttatctaa aacattaatt gctaagcgca
    44581 tacgatgcga gtaaacagta ttatgaattt tgagtgtgcc ttcaacgatt tgactttctc
    44641 ccagactgaa cgatatttca taagatattt cttccatttc gggtttttca gataaacgat
    44701 cttttaaatc tttaggaagt tgagcttttc cgtaaacaac taatgtttct tgtaaagaat
    44761 ctttatatac tttagctgtt tctaatttta gagatagttc tgtatctcct tctaatggtg
    44821 aataatcata cgtatcgcca tcatttgctg aatcgatcaa atgaacgaaa tcaagatatt
    44881 gtctatctcc aactatcaaa ttcaattgcc catctttaaa agaaagttta tatttattgt
    44941 tacttatctc agttttgtct atctcgattg ctgtttctaa tttttcatta tttttctcaa
    45001 attcaacaac agtgtatcct agtgctggca catttacatt tatttttatg tcaagttcat
    45061 aataagctgg ttcatcaaaa tattcgaaac ctgtagcaat catccgataa gcatttttcc
    45121 gttctggaaa ataaacttct ttttcaatga tattataggg actacctttg aagtgtattt
    45181 tctttgaatc agctactaca tgcacgattt tcgatccact ataattctca gtcgttgtgt
    45241 tgaaaacaag gacttcattc gattttagct ttaatgcatc agccatccgt tttacaataa
    45301 ggttttcgat tccgtcagct atctcattag cttctttgat tcgatggaaa atatcttccg
    45361 caacattatc tgaaacacag ccaccaatac tatcatgagc ctggttttgc aaaatctttt
    45421 tccataaccg acgaagtagg cctttactga tatggatacc atttctttct gcaatcagca
    45481 tgagcggctc aactcgtttt agaatttttt gttccaattt aaaattttct attttcattt
    45541 ttgttcttac tgagccaata gaacgatgaa cacgagagta aaccggctga cgaagttcac
    45601 ctttatattc aggtaattct tttttgtttc tgataacttc tacataagaa ggataatctg
    45661 aaataatatt gtggaattta ctgtatttat tcaacttctc tacgacttga tcaaaattta
    45721 aaatcattga cttttgatct acacccgaag gtactaaaat atctttgttt tttccttgca
    45781 tatgaagcat ttcaactgct tcatccaact tttcttttgc aaattcagga atatctttaa
    45841 ttgcgtctaa ggtcatcatt ccagtcatat aaccaaatgg aaaattcatg gcataaacaa
    45901 atttatctcc caatcctttc cagataaaat aaggagatgg aaccatcgtt tcaaagttta
    45961 atcctcgcca acacatgaaa ttatcgattt ctacttgatg caaaagtgta ggcaaattcg
    46021 cattgaaccc aaacgtgtca ggcaaatacc ctaccatcat tggatctcca tatttaagga
    46081 tcgtatcact gattccaatc attaagtttc ttaaaattga ttcagcatcc actaataagg
    46141 catctgtttg tgcataccac ggtcccacga atagttgtcc tgttttcatc agtcgttgga
    46201 taaccttaag cttttgcgga ttgatctcca aatactcatc gacgattgaa gtttgtccat
    46261 ctaaacaaaa gtttacttta gggttccttt ccaattcatc tagaacttca gtaaagacct
    46321 gatcagacaa tacgatggca tcttcatttg taaaatacca ttctctgtcc cagtgcgtgt
    46381 gttgaattat atgtgcattt accatatcag attctcctac tttccttctt ctactgaatt
    46441 tacttgttgt tcttttttat acttgatgga ttttagtgca ttgaccatta acgctgtaac
    46501 cactccccca ataacaattg atactaaata taaaggtata ttcgtaacaa aaggaattgc
    46561 cataaaggtt gaaagcattg tttcttgagt aacaccaaaa aatccattta gaccacatgc
    46621 tactgcagtg cccaccacga tgcttggtat tactcgtaaa ggatctttca cggcaaacgg
    46681 gatcgtccct tcagtaatcc ccacaaggcc cataatcata gctgtctttg cattatctcg
    46741 ttccgattca tcataaagtt ttttgtttaa aattgttgct aaaaacattc caaatggcgg
    46801 aatagcaatt cctaataaca tggctgtact aggtccaaag cttccgcttg aaaaagaagc
    46861 aaccccaaaa gcatatgcga ttttattgat aggtcctccc atatcaaacg ccatcattcc
    46921 tcctaaaata gccgacatca aagcagcatt tgctcctgac atgccttcta gccaattagt
    46981 cagtcctgtt gtcattgctt gaataggcgt agctagtaca taccataata tgattccaac
    47041 aactaatgta gtaacaaaag gaatcagcat aattgatttt agactaataa cttgcatcgg
    47101 tacttttact tttttcaatg cattagctaa atagccacaa gctaatccag caaccattcc
    47161 tccaataaat cccataccaa gattttgaca catcatccca gtgataaaag ctggtgctag
    47221 accgggacgg tcagcaattg aatatgcgat ataggctgaa atcatcggta cgataaactg
    47281 taatcccgtc tgaccaatca ttcgcaggac tccccagaat gtatccgtct ggtcccaaac
    47341 acctgtgcca ccaaaaatga ctgcaataga agttagtaac cctgctgcta cgacaagcgg
    47401 gatcatatag gaaaccccac tttggaaatg atttagaatt tctcttcctt gtttcttgaa
    47461 ttccattagt aactccccca cgatatattt tttatttgtt aattaatgca attgcttcat
    47521 caatcgtctc ttcaccatct tttactacct ctgaggtccc tactgttaaa atcggtttac
    47581 cgtcaaagcg acctcgttct gaaacatttg tatctactgc aaatatgaca acatctgcct
    47641 cggaaacatc cgaaattgaa agtttgtttt ctatccccat agccccctgt gtttctactt
    47701 taatatctaa ccctttcttc ttcgcaaact tttctaaatt agcttttgcc atataagtat
    47761 gggcaatccc tgcgatacat gctgttactg ctactatttt tgtcatatga aattcctcct
    47821 tttaaaatga tagagctttt accaattctt ttttatttct cgaatctttt attcttttaa
    47881 caaactcgtc atccatcaat cttccagata attctgctaa aatacgtaaa tgattagtac
    47941 ctttatcagt gtcttttatt gctaacaaaa agacgagatc aactggttga tcatctaatg
    48001 cgccccattc aacagaccgc tttaacttgg ctacagccac tgttgattca attactgcta
    48061 ggcttttgcc atgaggaatg gcgatatttt tcccaattcc tgtggtcata tggttttctc
    48121 tgtcaaatac gtctttttga aattgtttgt ggtcagacac aaacccatta gtctctaaca
    48181 atttcgataa tttttcgact acactttttt tatcagatgc atttaaatct atcactatat
    48241 tatttatatt gattttttca ctgatcattt ataagccttc tttcaatata taaactaatt
    48301 tttcaaacga cgaactcgtt tttaaatttg ctaataactc atgattcgaa gcgatttcat
    48361 tgaatgtttc ataaatttta ttcaattcag gttttacttt ttcattaagt gcaaaaaaga
    48421 aaacagaatg aacaagtgtc ccatcccaat caattccttc tggattaacg tagacacaga
    48481 ttactggctt ttttataaaa tctacttcga tatgcggcat agcaaaatca ttcatcgctg
    48541 ttgaagatag tttttctctt ttgattgctc cctctagtat cccttctttc gcgtaatttt
    48601 cttttaccaa ttttttggta attatattta aaacgttcac ttgattatcc tttatttggt
    48661 gggtaaaaaa gcaaaatcgt ttatctaata aatttagaaa agcatcttct gtttgttcat
    48721 ttaactgtag atactgtttt acttttctga catctgcccc taacatcaaa ggactaacaa
    48781 caataacagg aaaatttgta ttttcaatcg gaatcgtgct tataacaagt tcatcagtga
    48841 cttttctatc ttgtagttca ttgatgctta gtacatcttc tatctcaacc atatctgcaa
    48901 aaccgttatt tattctttgc tctaataatt ttgatgttcc atatccgcta ctacaaacta
    48961 atatcacttt cgttttgtca ggtttgtaac gatccaaaag tgattgaata tggagtgtta
    49021 cgtaagcgat ttcatcttca ttaaaatgta tacagaatct ctcttcgatt tcctctaata
    49081 aatctacact tatcataaat gaccttttaa aagaatattt gattttgtcc gtataagggt
    49141 tatagatatt tactcccaat ttgagtcttt taact
//
LOCUS       DAGCXG010000011        48767 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGCXG010000011 DAGCXG010000000
VERSION     DAGCXG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.011"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            87..389
                     /locus_tag="MB022_000629"
                     /pseudo
     CDS             87..389
                     /locus_tag="MB022_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            637..879
                     /locus_tag="MB022_000630"
     CDS             637..879
                     /locus_tag="MB022_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465652.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKDLLIKVK"
     gene            complement(984..2192)
                     /locus_tag="MB022_000631"
     CDS             complement(984..2192)
                     /locus_tag="MB022_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HBT4465653.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2482..3447
                     /locus_tag="MB022_000632"
     CDS             2482..3447
                     /locus_tag="MB022_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465654.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3469..3681
                     /locus_tag="MB022_000633"
     CDS             3469..3681
                     /locus_tag="MB022_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4465655.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3756..4232
                     /locus_tag="MB022_000634"
     CDS             3756..4232
                     /locus_tag="MB022_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBT4465656.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4229..4729
                     /locus_tag="MB022_000635"
     CDS             4229..4729
                     /locus_tag="MB022_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HBT4465657.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5289..7106
                     /locus_tag="MB022_000636"
     CDS             5289..7106
                     /locus_tag="MB022_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4465658.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7212..8540
                     /locus_tag="MB022_000637"
                     /pseudo
     CDS             7212..8540
                     /locus_tag="MB022_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            8617..8778
                     /locus_tag="MB022_000638"
     CDS             8617..8778
                     /locus_tag="MB022_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HBT4465659.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8819..9199
                     /locus_tag="MB022_000639"
     CDS             8819..9199
                     /locus_tag="MB022_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HBT4465660.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9214..9495
                     /locus_tag="MB022_000640"
     CDS             9214..9495
                     /locus_tag="MB022_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465661.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9754..10536
                     /locus_tag="MB022_000641"
     CDS             9754..10536
                     /locus_tag="MB022_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBT4465662.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10709..12427
                     /locus_tag="MB022_000642"
     CDS             10709..12427
                     /locus_tag="MB022_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBT4465663.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12429..13283
                     /locus_tag="MB022_000643"
     CDS             12429..13283
                     /locus_tag="MB022_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4465664.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13959..14279
                     /locus_tag="MB022_000644"
     CDS             13959..14279
                     /locus_tag="MB022_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465665.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14303..14713
                     /locus_tag="MB022_000645"
     CDS             14303..14713
                     /locus_tag="MB022_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4465666.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14860..15066
                     /locus_tag="MB022_000646"
     CDS             14860..15066
                     /locus_tag="MB022_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4465667.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15413..15934
                     /locus_tag="MB022_000647"
     CDS             15413..15934
                     /locus_tag="MB022_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4465668.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15992..16417
                     /locus_tag="MB022_000648"
     CDS             15992..16417
                     /locus_tag="MB022_000648"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4465669.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            16596..16826
                     /locus_tag="MB022_000649"
     CDS             16596..16826
                     /locus_tag="MB022_000649"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBT4465670.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            16860..17042
                     /locus_tag="MB022_000650"
     CDS             16860..17042
                     /locus_tag="MB022_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HBT4465671.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            17155..18666
                     /locus_tag="MB022_000651"
     CDS             17155..18666
                     /locus_tag="MB022_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465672.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            18989..19213
                     /locus_tag="MB022_000652"
     CDS             18989..19213
                     /locus_tag="MB022_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBT4465673.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            19206..19772
                     /locus_tag="MB022_000653"
     CDS             19206..19772
                     /locus_tag="MB022_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBT4465674.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            19760..19990
                     /locus_tag="MB022_000654"
     CDS             19760..19990
                     /locus_tag="MB022_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465675.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            20140..20556
                     /locus_tag="MB022_000655"
     CDS             20140..20556
                     /locus_tag="MB022_000655"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HBT4465676.1"
                     /translation="MNKPVLFVYRKKKEYKKMSRAKNPKYESMSLEELKANMEKSRQE
                     LEAAIHHKNILEQRKKLVERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQN
                     SSYNKSIVRQAHIAALHKDQQQIAEAVKKTEKGDDS"
     gene            20556..22283
                     /locus_tag="MB022_000656"
     CDS             20556..22283
                     /locus_tag="MB022_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBT4465677.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            22313..24229
                     /locus_tag="MB022_000657"
     CDS             22313..24229
                     /locus_tag="MB022_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4465678.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            24914..25096
                     /locus_tag="MB022_000658"
     CDS             24914..25096
                     /locus_tag="MB022_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465679.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            25123..26820
                     /locus_tag="MB022_000659"
     CDS             25123..26820
                     /locus_tag="MB022_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4465680.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            26845..26976
                     /locus_tag="MB022_000660"
     CDS             26845..26976
                     /locus_tag="MB022_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465681.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            27068..27931
                     /locus_tag="MB022_000661"
     CDS             27068..27931
                     /locus_tag="MB022_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465682.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            27968..28504
                     /locus_tag="MB022_000662"
     CDS             27968..28504
                     /locus_tag="MB022_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBT4465683.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            28518..28940
                     /locus_tag="MB022_000663"
     CDS             28518..28940
                     /locus_tag="MB022_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4465684.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            28870..31269
                     /locus_tag="MB022_000664"
     CDS             28870..31269
                     /locus_tag="MB022_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4465685.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            31301..33292
                     /locus_tag="MB022_000665"
     CDS             31301..33292
                     /locus_tag="MB022_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4465686.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            33315..33566
                     /locus_tag="MB022_000666"
     CDS             33315..33566
                     /locus_tag="MB022_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBT4465687.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            33556..34785
                     /locus_tag="MB022_000667"
     CDS             33556..34785
                     /locus_tag="MB022_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBT4465688.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            34782..36863
                     /locus_tag="MB022_000668"
     CDS             34782..36863
                     /locus_tag="MB022_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4465689.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            37012..40932
                     /locus_tag="MB022_000669"
     CDS             37012..40932
                     /locus_tag="MB022_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBT4465690.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            40933..41877
                     /locus_tag="MB022_000670"
     CDS             40933..41877
                     /locus_tag="MB022_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465691.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            41881..42084
                     /locus_tag="MB022_000671"
     CDS             41881..42084
                     /locus_tag="MB022_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBT4465692.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(42384..42620)
                     /locus_tag="MB022_000672"
     CDS             complement(42384..42620)
                     /locus_tag="MB022_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465693.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(42803..43189)
                     /locus_tag="MB022_000673"
     CDS             complement(42803..43189)
                     /locus_tag="MB022_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBT4465694.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(43171..44499)
                     /locus_tag="MB022_000674"
     CDS             complement(43171..44499)
                     /locus_tag="MB022_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4465695.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(44460..44789)
                     /gene="mobC"
                     /locus_tag="MB022_000675"
     CDS             complement(44460..44789)
                     /gene="mobC"
                     /locus_tag="MB022_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4465696.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(45047..45418)
                     /locus_tag="MB022_000676"
     CDS             complement(45047..45418)
                     /locus_tag="MB022_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4465697.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            45563..45748
                     /locus_tag="MB022_000677"
     CDS             45563..45748
                     /locus_tag="MB022_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4465698.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            45906..46073
                     /locus_tag="MB022_000678"
     CDS             45906..46073
                     /locus_tag="MB022_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465699.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            46691..47350
                     /gene="vanR"
                     /locus_tag="MB022_000679"
     CDS             46691..47350
                     /gene="vanR"
                     /locus_tag="MB022_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4465700.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            47353..48696
                     /gene="vanS"
                     /locus_tag="MB022_000680"
     CDS             47353..48696
                     /gene="vanS"
                     /locus_tag="MB022_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4465701.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILEPVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT        14014 a        10882 c        12728 g        11143 t
ORIGIN      
        1 tattaaaggg aaacattttt acagctataa tttgaaaaaa atttatcgtt attatatgat
       61 taagaaggat tgaataaaga aagaagttga aaacgatacc tgattacaaa aaaataactt
      121 ttataagttc tataacgttg attatatttt taattcttat tcttctcgtc gtcttggttc
      181 cccgaatttt tggtttgact ccttatatta ttgaggacaa agaaatgagt ccatattatg
      241 cacaaggaaa tctgatatat gtacgaccga aaaatacaaa tgagatttta gtcggagata
      301 ttattacata ttatgaaaat tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg
      361 aaaacaacca cgaagatttt tcctcaaaat tttaggttat cgctgaaagc cccggaaata
      421 cgggcatttc gcaggatatg agaactcaat ttactaccaa tttactactt tcggattgag
      481 ccgcatatat gctgaaccat actgctaata cccgccaccc caaatacggc acatcataaa
      541 ttgaatacga caccgtaaac gcggcgttcc tctttcccgt catgggagaa caggacgccg
      601 cttttttatg cccaaataga gaaaggaggc gacgccatgc tatttacttc cggccctgac
      661 cgggcgtttg aacggctgat gcaggaaaag cccggtttcg atcactacga cggcggcggg
      721 gcggacgcgc cggaggattg cggcacctgc cgattttacc gcccggattg gaaatatcaa
      781 ttctgccagt ttgcagagtg tccctatcag ccgggcaagc tgaccgacta tgcagcggtc
      841 aaattcacag tgaaagacct gctaataaaa gtcaagtaga aatttcagat ttttagggag
      901 gcgaaaaaat gcaatacaaa atcaagccgc tgagcatctc aaaattaaaa cggcggccag
      961 ccagatggta tagagtgtaa aaatcagtcg tgctccaggg attccaagta ggctttcaca
     1021 gaagcgtagt agatgcgcag gaacttgttg gcagaggcca tcatgtagac acgatatggc
     1081 ttgccttcag agcgtttctt gtccatgaac tggtacaccg gctcgtctat tggagcacat
     1141 tgcaggatga cgctcatcac cagaaagagc gtcctgcgca gtgaggcaga tcctcgcttg
     1201 gaaatgctac ggctacggac atctatttga ccggattggt agggcggggc gtcaataccc
     1261 gcaaaggcaa ccaacgcttt tttggaatga aaacggcgta catcgccaat ttcagccagg
     1321 agttgagggc cgagggttgg gccaacacca aacatcccca tcactacagg atactccggc
     1381 agagaagctg ccagggattg catctcctgt ttgagagcag ctaacgcgga agaagttgtc
     1441 tggagttgag aaatggcctg ttctaccaaa agttttgccg tatccgtttt cggtatgaca
     1501 ccgaagtgtc tgcaagcaga ggcataaata tccagcgcct tatcctcgct gaaattgtag
     1561 ccgtgctttc tgcaccactt ctggtattgg gtagtaaagg ccttctcaga ccggccacaa
     1621 acgcactcgc aatgccaaaa agtagcgaca aagtccaccc acttctcgct gccatcggcg
     1681 cggggcggac tggtaaacaa gcgatttgcg tctgggaagg tggtgtccag cagggagatc
     1741 aggttattct tcagcatggt ctgaactttg gaatactgtt ggtactgccg gtagcagatt
     1801 ttcagcatga gtcgggtatc ctcctccggg atatatctcg gcaatgtgag ccagtggtca
     1861 agaccgtagt tggccagctt tacggcatcc tttttgtcgg ttttggcccg tcttaaactg
     1921 ttgtttccgt agtcatgcac cagcattgca ttgactaccg agacataaag gcccgcgtcg
     1981 tggagcagcc aggccaccgg agcatggtaa ttacccgtgg attccatcac cacacgggtc
     2041 tcgccgtcca ggcttttgag cagtcctgcc agctcgctca gttcattggc ggtgtgacgt
     2101 acttcaaagg gtgaaaccac tacctctccg aagggccgca tgactgcaat catgctctta
     2161 cccttggaaa catcgatgcc aacgcagttc attcacattc ctccaataca aagaatctgc
     2221 aaccggaatc catcttttct cgctgccgat tcaatctatt gtgtgacacg aactctccga
     2281 catccggtgg ttcaacctgc ttaaatcgaa cgctgcaacg agaggatggc taacagtctt
     2341 tcacacggac atacaagccc aaggagtgat tggtcagcca gttgctcctc tcattgtagc
     2401 ttaggcacga gatggaggga aagccggact ggctgtccgg ctttcctgtc caaatttatt
     2461 gtaataggag cgaacaacgc tatggcagtt ttccgtgtgg aaaagaacaa aggctatacg
     2521 gtgatgtcaa atcaccacct gcggaataag aacctgacct taaaggccaa gggcctgctg
     2581 tcgcagatgt tgtccttgcc ggaggattgg gactataccc taaaaggact gtcctatatc
     2641 aaccgggaaa gcatcgacgc gatcaggaca gcggtatggg agcttgaaaa agctggatat
     2701 atccggcgtg agcagggccg tgacgcaaaa ggcaaaatgg ccgatatggt ctataccatc
     2761 tacgaacagc cggtattgga aaacccggta ttggaaaatc caacatcgga taacccggtg
     2821 ttggaaaatc cgacatcgga taatccggcg tcggaaaatc caacgcaatt aaataaagaa
     2881 gtacaaagaa ctgacttatc aaaaaaagaa aaaataatta cagatggaca aagtacccat
     2941 tccattccta tcccatcccc gacccctgcc cctatggagg gcgaggcagc ggctacgccg
     3001 ccggaacgga aaaggacagg aaaggatgag gctgtacgga tttatcggga aatcattttg
     3061 gagaacatcg agtatgccta tctcattcag gacaggagca ttgaccgtga acagcttgac
     3121 gaaatcgtag acctgatgct ggaaaccgtc tgcaccgccc gaaagacaat ccgtgtcgcc
     3181 ggggacgact accctgccga gctggtgaaa tccaagtaca tgaagctgac cggcgaacat
     3241 atccgctttg tccttgactg tctgcgggag aacacaacga aagtccgcaa tatcaaacaa
     3301 tacctgcggg ctatgctgtt caacgccccc agcaccattt ccaactacta cacggccctt
     3361 gtcgcgcacg acatggcaca gcccgattgg ggaaagccta aatccggcct gccggactat
     3421 tcctgcggcg agggcgaaag cctgtaacga gaaaaggagg acgaccctat gaagcagggc
     3481 gcattgattt ttgacgagcg aacagaccgt tacgacatcc gctttgatct ggccgactat
     3541 tacggcggcc tgcattgcgg ggagtgtttc gacgtgatga tcggcggcaa atggaggccc
     3601 acccgcattg aaatggccga aaactggtat cttgtcggca tccgggccga cgacctttcc
     3661 ggcctgcggg tgcggatttg acctatatct acctgcggcg tctggtggtt ccgccttaga
     3721 cgccgctttt gactttctga caaggaggga tgcgattgca ggaagaagtt actcaaaaga
     3781 ccattgccct gtccatgaaa acgggcaagc tcacggctca agtgctgcaa gcggcgctga
     3841 aaaagtacct gcaacaccgg gccaagggca aaaacacgct gcaccacggc cagcagagcc
     3901 taaagcagct taaaaagcat ggggctgatc tctccaacat tgagatcacc gaggccaaca
     3961 ttggagcgtt caagccatgc gccaagaaat acggcgtgga ttttacctta cgaaaagata
     4021 agaccaccca gccgccccac tacgttgtga ttttcaaggc caaggatgca gataaccttg
     4081 atctggcgtt tcgggagttt accgccaaga cgctttccaa ggagcagcgg ccctctatcc
     4141 gcaaggtttt gtccgctgtg aagcaaaagg cggcggccca gaccaagcag cgggccaagg
     4201 agaaaatcaa ggaaaggggg ctggaactgt gaagcctgac gtaaagaaat tgctgatcct
     4261 gaacctcccg tatctgattt ttgtgtacct gtttgcgaaa tgcggggagg cgtaccggct
     4321 ggccgctggt gctgacctgt cggggaagct cctgcatttt gcggatggtt ttgcggcggc
     4381 ttttgccaac ccgctgccaa gcctgcacct gtttgacctg tgtatcggta tcgccggtgc
     4441 cgtgatcgtg cggctggtgg tgtactgcaa gagcaagaac gtgaagaaat accgcaaagg
     4501 catggagtat ggcagcgccc gctgggggac ggccaaagac atagcgcctt acatcgaccc
     4561 caagtttgaa aataatatcc tgctcaccca aacggaacgg cttaccatga ccggcaggcc
     4621 caaagacccc aagacagcgc ggaataaaaa cgtgctggtg atcggtggca gcggttcagg
     4681 gaaaacgaga tttttcgtga aacccagtgc gcccgtaagg gcggtataaa tcctaccttg
     4741 agcaagtagt aggtaaaagt atcaagcggc attgtgccgc aacctatcat tccccgtctt
     4801 accccaaaag ggaaaccgga gggcgaccat agcatgacgg aggacacggg gcgctgaagc
     4861 ctcagaagcg ccggcgtgac ggaatgaaaa tccacgtatg aggatggaag ctagactgct
     4921 tgaatgacag cctgaaatcg ggaccaagag cgtacccctg acgtagagag gttgtactcg
     4981 tcagtgttcc tgacagtcgc attgtttggc tatgcggctg ctgatactcg gagcaggttc
     5041 agccacggga aagtggaaaa aggcgaacgt cacataccga caacgtggaa cgcttgttta
     5101 taccgtacta aacggggatt gcctaaagtg aaatgccgaa aggctatgaa aacgctgaaa
     5161 tgcggggttc tgaatattca ccatggcaac agagttccca tagtagtctg cggacgggaa
     5221 agccgtctac atggcaaagg ggaacagtga atcattttca aaacagaaag gatggtgtgt
     5281 gagacactat gagaaatccg attcatgtct tgaaaagcct tgaagaaaag gcaagtgtaa
     5341 gtaactacaa atatgaaaga ttatatcgca acctatacaa tccagagttc tatctccttg
     5401 catatgcgaa cattgcgaaa tcgcagggga gcatgacgca gggggtagac gggcagacgc
     5461 tggacaacat gagcctgccg agaattaacc ggattattga atccatacgc aacaggacat
     5521 atcagccaaa gcctgcaaaa agaaagtaca ttcctaaaaa gaacgggaaa cttcgcccgt
     5581 tgggaatcac ttctactgat gataagttag tgcaggaagt ggtcagaatg attcttgaag
     5641 caatctatga gccgacattc agcaacaatt cacacggttt cagaccaaaa agaagctgtc
     5701 acacggcact tacgcaagtg aagaaaaact tcacaggtgt tacatggatt gtcgagggag
     5761 acattaaggc gtgctttgat aacttcgacc atcatgtgtt ggttgaatta ctgcggaaaa
     5821 ggatttcaga cgaggctttt atcggtctaa tctggaagtt cctgaaagcc ggatatatgg
     5881 aacaatggca gtacaactgc acctactccg gtgttccgca gggcagcggt atcagtccga
     5941 tatgtgcaaa catctacctc agcgaactgg acaactatat gcaggaatac aaagaaaagt
     6001 atgattgcga gccagaacgc agaaggacga ccagagaata cgagcgggcg tcccggagat
     6061 acagaaaggc acgtaaagcg ttaatgggcg cagaaaaatc aactcctgaa ctggtaaaag
     6121 aatttaagga ttccagaagg aagaagatga atcagcacta ttacaatccg tttgaggaag
     6181 gcttcaagaa aatccagtac aaccgttacg ccgatgattt tgtaatcggc gttatcggct
     6241 ccaaaaagga cgcagaaaaa atcaaggaag atgtaaaaat ctttctccaa gaaaaactgc
     6301 atttggaaat gtccgaggaa aagacgaaag tcacccattc cagtaagccg gtacgctatc
     6361 tggggtacga tttcaaagta atccattcca agaacatgaa gcgttgtaaa aacggcgaca
     6421 tgaaacgggt gtggtatgga aaagtattcc tgtatatgcc gaaagaaaaa tggattaaaa
     6481 aagcgatgga acggggagcg atacaggtga aacgcaacaa tgacacaggc aaagaaatgt
     6541 ggcggcctat gccaaggaaa gacctgatga accgcagcga cgcagagatt gtgtctactt
     6601 tcaattccga aattcgaggg ttatataact tctatcggat agcagaaaac gtaggcgcat
     6661 tgcacaagta ttactacatg gtgcggtaca gcatgttaaa aactctggca ggaaagcaca
     6721 gaacgaatgt cagtgttatt aagaagcggc acatggtaaa cggagtactg agaatcccat
     6781 acgatacaac taagggacgt aaatactgcg aattttacca tgatggattc agaaagcaca
     6841 gcgacggcta tgacaatgtg gcagacgtta tgccgagtta taggaaatat gacagcaggc
     6901 acacgatagt aaaccgcata aaagccggag tatgcgagat ttgcggggaa catgcagact
     6961 atttatgtat gcaccacgta agaacgctga aatcgctgaa aggcagggat atatttgagc
     7021 agaaaatgct gaaaatgaga agaaaatctc tggctctctg ccctgactgc tttgaactct
     7081 tacacgaaac caaagaatca aggtgattca tggaaagccg gatacgctga gaggtgtacg
     7141 tccggtttgg gaggcggctc cctgaaacct actgatgaaa gtcagcaagg cgcagggtgc
     7201 ctacctcata cctgatgcaa tgctttccga ccacggacta tccgacttcg tttgtggtga
     7261 cagatccgaa aggcactttg gttctggaaa ccggcaggat gttccagcag gcgggctacc
     7321 gcattaaaat cctgaatacc attaactttt ccaagtccat gaagtacaac ccctttgtct
     7381 acatccattc ggaaaaggac gtgctaaagc tggtaaatac cctgatttcc aacaccaagg
     7441 gcgaggggga aaagtcagcg gaggattttt gggtgaagtc ggaacggttg ttttattcgg
     7501 ccctgatcgg ctatatctgg tacgaggccc cagcggagga aatgaacttc tcaacgctgc
     7561 tggaaatgat aaacgccagt gaagcccgcg aggacgaccc ggagtttcaa agccccgtcg
     7621 atctcatgtt tgagcgtttg gagcagaaag accccgacca ttttgcggtg cgccagtaca
     7681 agaaattcct gctgtccgca ggcaagaccc gttcttccat cctgatttca tgcggtgcca
     7741 gacttgcgcc ctttgacatc cgggaggtac gcgagctgat ggaggacgac gaaatggagc
     7801 tggacaccat cggggacgaa aagacggtgc tgtatctcat tatgagcgat acggacacga
     7861 cctttaattt cattctggct atgctccaaa gccagctaat caacctgctt tgtgaccggg
     7921 ctgatgatgt gtacggcggg cggctgcctg tccatgtacg cctgattttg gatgaatttg
     7981 ccaacattgg ccaaataccc aatttcgata aactcattgc caccattcga agccgtgaaa
     8041 tatcggcctc tattattttg cagagccagt cgcagctaaa agcgatttac aaggacgccg
     8101 ccgagatcat ttcggataac tgcgatagtg tgcttttcct tagtgggcgc ggcaaaaatg
     8161 ccaaggagat ttccgatgcg ctggggaaag aaacgattga cagtttcaac accagcgaga
     8221 accggggaag ccaaacgtcc cacggattga actatcaaaa attaggaaag gagttgatgt
     8281 cccaagacga aattgccgtg atggacggcg gcaagtgtat cttgcagcta cggggtgtgc
     8341 gcccgttttt atcggagaaa ttcgatatta ccaagcatcc ccggtacaaa taccttgccg
     8401 acgccgacaa gaagaatacc tttgacgtgg acagattttt gactatcacg cgccggaaac
     8461 ggcagcaagt ggtaacgcag gatgaagtat tcgacctgta tgaaatcgac ctgtcggacg
     8521 aggacgccgc cgctgaataa acagcggctt ttttcattcc aacaacaaaa tcaaaaaatc
     8581 attatgagcc ggaaaccggc agaaagaggt aaatttatgg cattttttaa ttccgcagtt
     8641 actactttgc agactatcgt gatcggactg ggcggcgctc tgtgcgtatg gggcggcatc
     8701 aatctgttgg agggatacgg gcaggacaat ccgggggcaa aatcgcaggg tgtcaagcac
     8761 ttaatgtctt ctatgtgaaa agattttaaa gagaaaatgc acatcctacg cagataacat
     8821 gacaatttca tgcaaagcga aaggagacac catgaaaatc gaatacacaa aagtgggaga
     8881 ctattattta ccgaatcttt actacccgga ggaaaccaga ccgattggct tatggggaat
     8941 gcttcggaag gaatatctga aagaacataa atctggaact tatacatatc ttcttctgac
     9001 cgccaggctg gacagttatt tggcagatct gaatgaacag gcacaggaac gctttgaatt
     9061 gatcgaaact cagatgcgaa gtgcggaggg agtttccgag gaactgaaga gacagaatcc
     9121 tatggaatgg ctatgccact gtaacaatat ccgcaaccgg gctgctgaga tcatcaagca
     9181 ggaactgatt tatgtataag ggggattcta gatatgagaa gagaactgat tgaaagttat
     9241 tatgaagaat accagcggga gctggaaaga tttcttgaat ctgacccgga gatgttcact
     9301 ctggaagcag aaattcatca ttacatagaa atactggaac cggtcctcag agatggcgga
     9361 caggagatgt ggcagacact ggacaggatt atttcagcga aaaatgcaat ggagggaatt
     9421 gtggcaaaag aaatgtatat ccggggattt ctggattatg aaagactggt gtgtgaaaaa
     9481 gacagtaaga tctgaataat cagaacatag actatatata acgaggcttt tattgtatag
     9541 acagtaagag cctcatttaa tatagttttc ataataaaaa acaggtggta gtacgtattt
     9601 gctttgtgtt atataagaat tggtctggct gaacaatggt gaaaatttac tatgatttta
     9661 cttaaatctg attgaaggat gaaaaagtga tgatatcata tagtgggttg atagggttac
     9721 agaaaatgta ttcacagagg cagagaagaa ataatgaaag taaaaaatgc agatgaattt
     9781 tataaaatat tagaaagaaa ggctatatat ccggtatttc agccgattgt aaatttacag
     9841 actggagatg tggcaggata tgaagctctt agtcgtatcg acaggcatga tacgactctt
     9901 atgatttcag atttgtttgt gattgctgaa caggtaggct gtgtttggaa attggaaaaa
     9961 ttgtgcagaa ataaagcatt gaaggcagcg gcgaataaac cagaacatgc aaaattattt
    10021 cttaatgtag atggtaatat cattcaggat aaatcgttca tacagggatt tacaaaccgt
    10081 aaggctgcga aagccggagt accgtcctgt gacattgtat ttgaaataac ggaacggtcg
    10141 gatatagaaa actatcagat tctgcaacag attatgaagc attatgcaga tcagggatat
    10201 gaaatagctt tggatgatgt tggagctgga tattctggtc tgaaccgtgt agtaaataca
    10261 tcgccaaact acttaaaagt tgatatagaa ctggtcagag atattcaaaa acataagaaa
    10321 aaggaaatta tgatggaatt tctgttgcat tactgtaatg agacaggagc aattctgatt
    10381 gcagaaggga tagaaacaga agcagagttg gagtgtctgt gtagattagg tgttcattac
    10441 ggacaaggat attttcttgg aagaccggac agaacgttca aaaatgtaag gaatgaggcg
    10501 atgttggtat tacgtagact gaataataaa tcgtaatgaa aactatatgt cagcaggtga
    10561 aaaatgaata agtcaattat aacattataa agatttccat tgatttttac acctatttta
    10621 cacaagcact cacatagatt tgatttctgt cttctataat tgtataggaa aatgtagatc
    10681 ttatataata acaggagagg aaagagaaat gaacgagaat gtgaaagtgg tttttggtct
    10741 gatcggtggc cttgccttgt tcctttatgg aatgaacagc atgagtgatg cacttcagaa
    10801 agccgcagga gaacgaatga aaaaaatcct tggatttctt acaagaaatc caattatggg
    10861 agcacttgcc ggagcacttg taacagctgt attgcagagc agttcagcga cgacagttat
    10921 ggttatcggt tttgtcagtg cgggacttat gagtcttccg caggcaattt ctgttatctt
    10981 tggtgccaac ataggaacca ctatgacggc acagctgatg gcatttaaaa tcagcaatta
    11041 tatttatcca attatctttg ttggatttat tctgaacttt gttagcaaaa aagaaaaggt
    11101 taaaaatatc ggtatggtaa ttttttcatt cggactttta tttgaaggaa ttgaaatcat
    11161 gggtgaagtc atgaaacctt tggcgggaag tccggtcttt gttgatctga tgggaaaagt
    11221 ttcatccatt ccggttcttg gtgttgtact gggagcggtc atgacattgg ttgtgcagag
    11281 tagttcagct acgattgcag ttctgcagaa ttttgcatca caggcaggac cggacggagt
    11341 aagcagtgta atagggctta ccggtgcgat tccgattctt cttggagata atataggtac
    11401 aacgatcaca gcacttcttg catcaattgg acaatctaaa aatgcaaaac gtacagcaat
    11461 tgcgcatagt atttttaata tcagtggaag ctgcgttttt attttcctgg ttccgtggtt
    11521 tgcaaaattt gtccagttta tttcaccaaa gggaaatgag attgatgtga tttcaagaca
    11581 gattgcaaat gcacatacaa cgtttaatat cgtctgtaca ctggtctggc tgccactgat
    11641 tccgctgatg gtaaaaattg ttacaacaat catccgtgga aatgacaaaa cagagaaagc
    11701 agcttttgaa cccaaatatc tggatatgaa agtgattgaa cagccggcgg ctgctatggt
    11761 tctggtatct aaggaactga accgacttgg agaactggca gaatctctgt taagtgattt
    11821 aaaaacggcg attgtagcag atggagactc aaaaactcat ggttcgttta tagaaaatct
    11881 ggagattgta catcagttac aggacagtgt gacagaatat attaccagat tattcgcaag
    11941 cggaaacctt acagaacaac agtctgagca gacggcaggg ttactgtatg taaacaatag
    12001 tattcagagg attgcagaca gatgtgaaga tattgaccag atatgtgaaa aagtaaataa
    12061 caatggaaaa agactgacca gtgaagcctc ctcagaaatg gaagactgca ttgatattat
    12121 ccagcagctt ttaaagaaag caatggaggc aatccgtaag ggtgatgctg tagtggctga
    12181 aacagttttt aaaaataaga aaaaaatgca taaagcagag aaaaaataca gtaaggcaca
    12241 tttaaaccgt gtaaaaaatg aggtttgtga tgcgtctatg acaagatatt tttctgggat
    12301 catgtataat tttgaccgta tggcagataa ttgtgtcagc attgcagaag aagcctgtga
    12361 taatgtggct tttataaacc ttgatgaaga gacgggaaaa tcaatgatgg aaagaggtgc
    12421 agtataaaat ggtaaagaaa aaaaacatcc gaaaatctgc acttctgctg ataatgataa
    12481 tgctggcaac gggaataaca gcatgtggaa ccgaggaaga gccattgccg gatctgtctg
    12541 ctatgggagc tgtcacagct gtatcacgag aagaagggtc aggaaccaga gcagaatttg
    12601 agaatctttt gaaactgcct gagagtgata caggaatagt tgttgattct acagagaaag
    12661 ttctgaaaaa ggtggaagaa gataaaaacg cagttggtta tgtcgcatat agttccgcaa
    12721 cagatacgaa tggaaaaata cttcaaatca acggagttct tccttctgag aaaacaatag
    12781 acaataacag ttatcctctt tgcagggatt attatctggc ttataatgga gaactgacag
    12841 atgtggaaca ggatttcctg acttatgtaa agagtaaagg acaggatatt gtgaaacaat
    12901 attgtattca ggctgacagt acaacaacat tcctttctga caagtcagaa ggaaagattc
    12961 tgattgaagg atccacttca atggaaccaa tggtaaaagc actggcagat gactatcaga
    13021 aacaaaatcc aaatgctgaa atagaagtaa aagcaacaga ttcttcaaga gggattacgg
    13081 cggtgataag tggagagtgt gatttcgcaa tgtcatccag agaattaaag gattatgaag
    13141 cagagcttct ggaaacaaaa gtcattggaa aagatgctat agctattgtg ataaatgaag
    13201 aaaatccact ggaaaatctt acaataaaac aacttacagg tctgtacaat ggaacatata
    13261 aaaactggga tgatctttcc tgagaaaaac gaatataata tcttacaagc actgactata
    13321 acggtcggtg cttctttttt ggattttttc aaaattatct gacattcttc aaattcccac
    13381 gtgtgattag tggaaaaata attttttctg caaaagtacc gtccaaatgc cctctgaaac
    13441 ttgctattag tgagggaata tttcatttat gcgaaaaaaa cttgcaaaag ggggttggta
    13501 aataccgctt cccaggcttt ataggtagag gaacaattat tttaaaaatt ttaccccaaa
    13561 gatgtccgct tttgcctgtc tcagtacaga gtatgtgaag ggactaacaa aagtaaaaga
    13621 aaaaatgata aaaatgcaaa aaaatatcac gaatcttttg catcttgcga agtcatttga
    13681 ctctagtaat cagaggacgt tttggaatgt tccttcgatg aaccttgaca accgataagt
    13741 gcaccctaga agcctaacag gagttatgcc atgacctttc tttttctgag agcgagcgaa
    13801 attataatga cactcccgat gatgggcggc tgaccgaagc cttatggtca gtgacctgct
    13861 gatgcaggca gaggcagtct gagacggctg caggtgacag ggaaacgcac gccagaccgc
    13921 cggacgaatg aaactgatga caatgtttac agggcaaaat gcccggaaag gagcagttta
    13981 tgacagaact gaaaatgaaa gaaatgcagt tgattaactg gtttggatgc ccgaatctga
    14041 tttatacaag ggagagaatc catctggttg ccatgcttac aacagatgaa gaaatgaaaa
    14101 agaatcttta cagggtatgc agattccttg caagagaaga aatcgcatat cggtatccga
    14161 tcatgtatga aatgatccgg aaaatatttt caccggatga taccaatcca ccggaagcat
    14221 gtgcaatgtt tatgccggac gatgaaacag atgatgaaga atatgcaatg gcagcgtagc
    14281 tgcggaaagg acagtaacga ttatgaataa agtggtttta atgggaagac tgacaaagaa
    14341 tccggagatc aaatatgccg gaaaagataa tgatatggca gtagcaagat atacactggc
    14401 ggtaaacaga agatataaac gtgacggtga acaggaagcg gattttattt cctgtgtcac
    14461 attcggaaag agtgcagagt ttgcacagaa atatcttcac aagggaatga ggattgtgat
    14521 tggcggtcgt atcagcactg gcaattataa ggacaaggac gggaaaacaa tttatactac
    14581 agatgtgatc gtggaagaac atgaatttgc ccagaacaag gataatggtg ttggagctga
    14641 ttcatcggaa acaccgaaaa cagataagga cggatttatg gaagcaccgg aaggtgaggt
    14701 tccattcgac taaaccgaag taaatggttg gagaggttgc cctttgtcgg ggcatcctct
    14761 tttccatttt acagaagaca tcaaaaatta caggtattca acctaacgaa atcgtgatat
    14821 aatcaaaata tgttcagatg aaaaatatgg aggttgctta tgggcgtaag ttataacaga
    14881 ttatggaaaa tgctcataga taaaaatatg aagcggatag agatgcagta cctcaccggg
    14941 atcagtggaa atattcttgc caggatggga aaaaaccagt atgtatcaat ggagacgatt
    15001 gagaagatct gcaagaagct ggactgcacc gttgatgaga tgctggaatt tacagatgat
    15061 gagtaacaag agcagacatg tcaaattatt aaaactggaa gaatgcttca gaagtcgaaa
    15121 aaagaaattg aaaaaagata acacgccgga tacggcataa atctcttagc aacgctaaga
    15181 gcgcacttct atacaggcaa ttccatctgc ctgtatcgtg cgacctgcgg tgctgttccc
    15241 gatttcaaga aatctcaaac cgggaaatac agcgaaatca gagatttcgt acaaggggaa
    15301 gatccccttg cggcacagat gcctatccct gataaagttt gtgactgaat atttcagata
    15361 tagacatata aggggaaaga aagtgtgaaa tcaaaatttg tggagagatt ttatgattag
    15421 aaaagcaata ctaaaagatg ttaattcgat tgaagatact tacaatgagc atttccagta
    15481 tgaactgaat catacagcct ttactgtatt taagaaaggg gtttatccaa cgaaagatga
    15541 tgcagaacgg gctatttatg ctggtgcttt gtttgtatat gaagaaaacg gaacaattgt
    15601 tggaagtatc attattgata aagttcaacc aatagaatat gcaactattc cttggaaaga
    15661 aaaattatct gaagatgagg taatggtaat tcacctttta atggtacgtc cgagtatgtc
    15721 tggtaaagga attgcatcat cactaataaa attcgctaca gaattagcac aaaaaaatag
    15781 ttgcagggca ctacgtttgg atacaggaag tcaaaatatt cctgcgctat ctttgtatca
    15841 aaaaaatgga tttgaaataa ttgctagtgc ttctaaaaaa gttggagatg taattgcaca
    15901 caaaaaccat ctttttctcg aaaagatatt gtagttcgta aattccagtt tgaaaaattg
    15961 agaaaatcgg agtttgagga gcatcaccgt tatgattaga gaattacgga aagtggatat
    16021 aaataaagtt gcagaaatat ggttagatac aaatataaag acacattatt ttatctctgc
    16081 tcaatattgg aaaagtaatt ttgagttagt aaaagaactt ctattacagg caactgtcta
    16141 tgtttacgag gataaacagg aaatacaggg ttttataggg ttaagtaacg aatacattga
    16201 aggtattttt gtttctgccg aaatgcaatc tcaaggtata ggtaaaattt tgctaaacta
    16261 tgtaaaggga aaaagaaaca aattaatctt aaatgtatat caaaaaaata cacgggcaat
    16321 atccttttat caaagagaag gatttgagat tcagtatagc ggtttggatg aagctactgg
    16381 agaaaaagac tatgtaatgg catggcagca gaaatagaaa ttcaagtttt agtgtttgga
    16441 aaattatgac ttagtgtgca agttgggaga aaatatggta acggaaaaag agaaaaaagc
    16501 atgtcgattt attgccgaat ggctataaga ttgtgagaca gtctctggta gatgatctgg
    16561 agggcgaaaa aaaggtatgt tgggagacag aaattttgca ggatgatgat agtggattta
    16621 cttatgaaat cagcaggtgt ctgtatttcg atacctgtaa ggaacatggc tatgcagagt
    16681 tctgcaaagt attttgcaac catgactggt atgcttatgg agtattaaag catcattcga
    16741 gatttatcag aaaatcgacg attgccgaag atggaactgt atgtcatgat acaatcgaga
    16801 aagtaacaga gaaacatttt ttataaaacc gaaaaattga ttatgggagg aaattgaaaa
    16861 tggcgaaaga agagagattt gaaaaaatat attctcaggg agcattaact actactgaga
    16921 tatgggtgga taaggaaact ggtgtgaatt atttatatca tgcatgtggg tattcaggtg
    16981 gactgactcc attgttggac agagatggga aaccagttat ttctcaggct ataaatagat
    17041 gaccttagaa acagcaactg ttatggtaag taacagcatc gtcacaatat gtatatggtc
    17101 gattggtgtg tgcggtaata tactatattc agaaaaacaa ttcaggagat ttttatgaga
    17161 agatcaggtt ggacaaaatg gttgatcgtg attccggcaa tgctaggcat tgtgttggtg
    17221 acctatgtgc ggtatacaac ggatatctgg ggatttggtg catttatctg ccagctgatt
    17281 gcaattttgg aattggctta tggtctgcga attgcgatgc tggcacagaa tagaaagaag
    17341 tcctatcggt taacaccgga agaacgacat gagtatgcac ggtatttata tgagaagcaa
    17401 tatcaccgtt accctgctgt agcgaatcag atgcttttgg ttatggcacg gatgagtgta
    17461 ctccttaaca attatgaacg ggctgcacag gagctggcag atattcgtat agataaattc
    17521 aatccagcac agttgaagtt gtattattac ctgaaagtgg ttacagcaat ggcagcagga
    17581 gatgcgacag ggattcaaga gagccagatg tgttacgcag ggattccgga tactaaatgc
    17641 acatatccgt caaaagcaga gcttgctatg tggatagagc agagagcggc agcgcagatg
    17701 gcagaggcac tgaagaaggc agtgccggat aaaaaagagc atccgatacg gatcagtatc
    17761 attactttgg ttcttgcgta tagtaccttc ttctatggat tgtggtatgg tataaaacgt
    17821 gatatgggat atgaagtgag atatcgtttc gcagaaatat cgattactgt tattacaatc
    17881 agtctggcgg ttcttttgat ctgggggatc gtgagtttat acagacacaa ttgccaggca
    17941 ttatccatac aattcggtaa aaacaaagtg gcattgataa ttgtctatgg agctgctgcg
    18001 atatttgcac ttttgattat tggaatgagt ggtctgagtg tagtgttcgg aatggatgga
    18061 acagaaacgg tgactggttg tgacagcaaa tatacgtata taactgtgca ggaagattat
    18121 ggtggtgaac ggagatacag aaccaataat ccatttgtta tgcagagtat ggatggtctg
    18181 tggtggtctt cggatacaat agatgaagaa gataatcaag aagaggatac tacagaatta
    18241 ccggatttgg atacagagaa ttcaacagat tcagaggata ttcagagtgg aaataccata
    18301 gaagattacg agacagaagg gcagcatgat ggtctgacaa ttcaaaatgc aatgctggca
    18361 gtttataatt accttcagga gcagaataca cttcaaaata tggcgttcag ttacacggcg
    18421 aatgcaaagg gagaagtcta tgctgtcgtc agcgaaacgc aggaaacaaa gaacggtaat
    18481 acggtaaatg tgagatactg tctgtacgat aatggttcca agacagatgc agatggtaat
    18541 tcctgcgaag aactggtgtt agaaaaagta tatccggatg gcaattacga gaccgaattg
    18601 gtagattttt atctggtaaa tgcagatacg atgcaggtga cagacgaaca gaagaatacg
    18661 tggtaaaata aattctgggg aagtaggtgc attgtatgac aatgagagaa aaaataaaac
    18721 tattatcgta aatgtttcaa gcggactggg ttcttttgga agtatgcaaa aggactccaa
    18781 gacataagag gtgaactgcg ctgatccagg tgccacggca acagatttga atgtcgacgg
    18841 aggaaagcaa acagtacagg cgcaatcgta aaacttgcaa tgattgataa aaccggacta
    18901 acagtaactt ttattagtcg atttggaaat atgccttggt aatcaacaat tccggtttta
    18961 ctgactaaga aaatgaggag gattcataat gagtgagaaa acagagatta cttttatgca
    19021 aacaagacta atccggcttg cttcagaaga gtggcattta cctgttgaac agattatcca
    19081 cttgtttaaa gaagccgatg ttcttggata tatagaaaaa tgctatggga tttttcattg
    19141 tgaaggcgat gaagctgtgc ttgaagatat tactgaattt ttgcaaagaa aggggataga
    19201 gattagtgct tgagttaaaa gatggatttg ttctctatca tggaagttat tgtgaagtaa
    19261 aagagccaga tcttgccaaa tgtgcaaaga gaaaagattt tggacaggga ttttatttaa
    19321 ccacatcaaa ggaacaggca gaaagttttc tgagaacctc aatagccaaa gccatcgcaa
    19381 ccggaacaat tgaagagggg cagaagtttg gatatatttc aacttttgaa ttcaaacttt
    19441 cagaaaatct tgaaacgcat atttttgaaa atgcagatgt ggactggtta cattgtatcg
    19501 ccgctcaccg aaagaaaaaa atgtttattg aggttgaacg tgaaatggca aaatatgata
    19561 tcattgcagg aaagattgcg gatgatgcaa caaacgctac tcttaccgct tatcttgcgg
    19621 gtgcttttgg gactgcaggt gataaagtag cagacgattt ctgtatcaga cagttgctgc
    19681 cgaataaatt gaaagatcaa tactgcttta aaactgaggc agctattgag tgtctgaaat
    19741 ttgtgaaagg tgagaagata tggctgaagt aacaattcca aaggaaaaaa tgaattatac
    19801 aatcgacctt ctgatcacta tggtgacaga tgaaatcgca gaagagacag gcaaggacag
    19861 aaacgaggta ttgacagatt ttttgtgttc aaagacagga aaagcactgt atgatgagaa
    19921 gacaaagctg tggtgcactg gtccggcata tatagctgaa ctgtatatgg aagaattaaa
    19981 gaaatcctga tatgggatgt gagtttaaat ttatacgaca atggtgacaa agactttcat
    20041 tccaaggagc aacaattaaa tatttcaaaa acgctgaggt cagcatttcc taatcacagg
    20101 acaacgacct cagtttttta tacccaggaa tcggcattca tgaataagcc ggttcttttt
    20161 gtttacagaa aaaagaagga gtacaagaaa atgagcagag caaaaaatcc aaagtatgaa
    20221 tccatgtcat tggaggaact gaaggccaac atggaaaaga gcagacagga actggaggca
    20281 gcaattcacc acaaaaacat tctggagcag cggaagaagc tggtagaacg cagggaacgt
    20341 tcccaccggc tgattgtcaa aggggcagag tttgagaaag tatttccgct gtccagagat
    20401 ctggaacagg aagacgtcca ggacatgatg aatcaactgc agaacagcag ttacaacaag
    20461 agtattgtgc ggcaggctca tatagccgcc ttacacaaag atcaacagca gattgcagaa
    20521 gcagtaaaaa agacagagaa gggagatgac agttaatggc actgtatcat ttttcagtaa
    20581 ggaatgtatc cagagggaaa gggcagatgg tggtcgcttc cgcagcctat atttccgggc
    20641 agaggatttt tgacagttat tacaacaaga tacacgatta tacgagcaag tcaggtgtga
    20701 tatttacaga aatcctgaca ccggagtatg tgccagagcg tctgactgac agggaaacac
    20761 tctggaacga ggtggagcat gtggaacgga acaacaaggc acagcttgcc tattcgtttg
    20821 acattgcact gcagaatgaa ttgacacttg aggaaaatat cagactggca aaagagttct
    20881 gccaggagca gtttgtggca cgtggcatga tcgtggatct ggcagtccat gagggaaaag
    20941 cggaaaatga agaagaaccg gataatcccc atttccatgt gcttgtccca atccgtcctt
    21001 ttacagagga aggaatctgg ggcaataagc agagaaggga atatattctt gatgaggatg
    21061 gaaaccgggt gaaggatgcc aaaggaaaag atatgttcaa tgctgtttct actaccggat
    21121 ggaatgatcc ggagctttta aaagaatggc ggcgggcatg gacagagaaa gtaaatggga
    21181 aattcaggga gtgccacatg gcatccagaa tcgaccaccg ttcctacaaa gaacagggga
    21241 ttgacctgat cccgaccatc catgagggat atgaagtccg ggcaatggag aaaaagggaa
    21301 tcaaaacggt gatcggagaa ttgaaccggg caatccggca gtttaaccag atgtttattt
    21361 ctctgaaaga atccattcag tggatgaaga cagcttatga ggaaatgaaa gcagagctgg
    21421 atcgcaggca gaacccgaca ctgctggaaa gcctgcagga ttattatgat aaaaagacac
    21481 agggcagacc accacttccc aatttttatg cggagatgaa acagagaggt aagaatctct
    21541 ccaatttaca ggagtttgca aaatccatca gttatcttca gacacatcag atagaaacga
    21601 tggatgactt gcaggaacgg attgaggaac tgaacggtgt tgtttctgtc agtaaaaaag
    21661 aaatttctga aaagcggaaa cagttaaagg aacttgagaa tctggaaaag atggcagagg
    21721 taataaaaac aaatcagcca ttgattgatg agtataacca tttctatttt cagaaaagac
    21781 gggagagata ttatcagcag cataaaaaag aaatcaatta ttaccgcaaa tgtgaacggg
    21841 aactgaaaca acatctggat aaaaatggaa aagtgccaac tgccagatgg aaacgggaga
    21901 aagaagaact tcaggcgggg atcgaagagc tgaaagcaga taatcagccg taccaggagg
    21961 aactaacttt tgtgaaaaag gtacagagct gtgcggatat tgccagacgt gaccgggaaa
    22021 tggcagaagc agatacttcc gggcgttcag aagaaaaaag ggagaagcag gtgaagttcc
    22081 cgacatttta cacagttcag gcgaaagaga ttcttgaaga aaacgggaaa gcagagcaac
    22141 agccgttgaa ccagtcggaa cagaatccag aaaagaagac ctctcttctg agaaaactgg
    22201 atgaaaaaaa gaaagagtgt gcagagcgtg atgcaaaaca acaggcagtg aagaaaaagc
    22261 gaaactatga catgagttta taacagtctg tcagcaggac agacgggaaa ggatgagcaa
    22321 aatgcagtat acagaagaac aaattataag agcaaatcag acagatcttg tttcttttct
    22381 gagcgcacag ggagaacaac tagggaaaag cggaaaggaa taccgttgga aaaagcatga
    22441 cagtgtaacg gtatccggaa accggtggta caggcacagt caggggaggg gtggctatcc
    22501 ggtggatttt gtgatggaat tttacaatgc cacatttccg gaagctgtca agatgctaac
    22561 cggggaggaa ggcgaaggca ggaacagtac ctgccccgcc ccgtcccctg acttccgatt
    22621 accagagaaa gaggaaaata atgatagaat catcagatat ctgacagaaa acagagggat
    22681 tgaaaagaat atggtggagg aatggatcgg cagtggggat atttatgaag aaaagaagca
    22741 ccataatgtg gtgtttgttg gcagagatgc agacggtatt ccaagatatg cccattgcag
    22801 gggaactggt gaaacaaaat accggggaga tgtggctgaa tctgataaat cttacggatt
    22861 ctgtcacaga gggacggata atcagttgtt tgtgtttgaa gcagccattg atctgctttc
    22921 ttttatccaa ttatttccca aagactggaa aaagcggagc tacctgagtc tgggtggcat
    22981 ttccagtgcg gcactgatgg cttttctttc tgaacgccct cagattactt cggttttcct
    23041 gtgcctggat aatgaccatg cagggaatga agccagtgag aaattggcaa tagaaatccc
    23101 ggacggatac agtgtcatcc gtctgaaacc gtcccgaaag gactggaatg aaatcctctg
    23161 tgataaaaat gcagacagga agaaatcaat cattgaaaca gttacaatga aagtaccgga
    23221 gaaggaagaa ctggtgccga tgttgtgtta tgaagacatt gaacagacca gtgtagaatg
    23281 gctgtggttt ccctatctgc catttggaaa actgacgatt attcagggca atccaggaga
    23341 gggaaaaact tattttgcca tgatgctgac cgcagcctgc acgaaccgta agactttccc
    23401 gaacatggaa gagattgaac cttttaatgt gatctatcag actgcagaag acggtatggg
    23461 agatacgatt aagccgaggc tggtggaagc aggagcagac ctgagtcggg tgatggtcat
    23521 tgatgataca gaggaagcat tgacgttatc cgatgaccgt attgaaaaag caatccgtca
    23581 gaatcaggtc agacttttaa tcattgatcc ggtacaggct tttatcggtg ccgatgtgga
    23641 tatgaaccgg gcaaatgaag tccgtccggt attccggaaa ctgggaatga tcgctgaaaa
    23701 gaccggttgt gcgattgtgc tgatcggaca tctgaataaa tcttcaggga cacagagtac
    23761 ttaccgggga cttggttcta ttgatatcat ggcggcggtg agaagcctgc tttttatcgg
    23821 gaaagtaaag aaagacccga caacaagagt actgatccat gagaaaagtt ctcttgcacc
    23881 tccaggtgag acgatggcat ttaaacttgg agatgaagaa ggattccgct gggtgggagc
    23941 ttatgagatt tccgcagatg atcttctgga cggaaaggaa ggaaagccga cagaaacaaa
    24001 actgcaacgg ggaacgaaac tgatctatga actgcttgca gacggaaatg cagtcacaat
    24061 ccgggaactg gatgaaaagg caaaagcaca gggaatctcc cagagaacca tgcgagaagc
    24121 cagaagccgg atgaaagaag aactggatta tcggatgaat gaaaagcagg aaaataccat
    24181 tcgattaaaa aaacaaggga gaatgggtga cggcagaatt ttggagtgaa cggcaggctt
    24241 tatagatacc ctatctgccg tctgccgtct gccggtgcaa ggcaatcaaa cggaaatagc
    24301 aggtatccat acagaataag gattgctgtg aatctggaac aaaaatgaat tgtaatatgg
    24361 cggatataat ggtatgatta tccggtaaca gcaatgttac ggggaagcac ctgtgacagg
    24421 gtgtggctgc ctcctgatat ttacagaccg gagcctttcg gggttctgag atgaaagaaa
    24481 ggaggtgcgt gatatgacag cttatgagat tatctcaata ttcttaggcg tactcagttt
    24541 actgatcgct tttggtagcc tgttaattgc gattattgcc tttcttgatc ggaagaacaa
    24601 gaggaaataa aaatgcctat cctgtctgcc aacaggatag gcggtggtcg taagaccatt
    24661 tagtcaactc aggcggcatc cactttggag tgggtgcttt tcctataaca aatgtaccac
    24721 acattcctct tgttttcaat gggaaaatta aaatatgcag ttcatctgac agggatttat
    24781 ccctgtcttt attatatccc caatttctga aatgttaata caaaaatttt aatatatgta
    24841 accaacagtc tgggcagtta atctgtaaaa atggattagc tgctcttttt ttgcaaaaaa
    24901 tggaggtttt acaatggcag tgaagaagaa aattatcaac atcagagtat attttgacgg
    24961 tgatcaggac gcaaaagaag tgtttgcaga ggtgatcgca cagaaaatac gtgacacgaa
    25021 aacaaaagat aaacttgcaa taatgaaaga taagcagtat aatgattatg agttcagtaa
    25081 tctgaaatct gcgtaggatg tgcaaagtaa cggaggtatc agatgaacaa cagatatatg
    25141 agtaacaata tagaaagaaa tgcaacctgg agagttggtg tatactgccg actttccaaa
    25201 gatgacgaat tgaccggaga gagtgccagc atctccaacc agagagacat actggtgaat
    25261 tactgtcagt cacagggctg gcaggtggtg gatgtgtttc aggacgatgg ttatacagga
    25321 ttgaacacag accgacctga tttgcagaga cttctcaaag cctgtgaaaa aggactggtg
    25381 aatcttgtaa ttaccaaaga ccagagccga ctgggacgaa accatgtaca gacaggattt
    25441 ctgatggagg agtttttccc aaagcatgga gtaaggtata tcgcattata tgataatgtg
    25501 gatacctttg ccggagacaa tgagattgcc ccatttaaga acgtgctgaa cgagatgtat
    25561 tccagagata tttccaagaa agtccatgcg tcctatcacc tgcaggcaac caagggaaag
    25621 tttacaggtg ttgttccacc actgggatat cagaaagacc cggaggaaaa gggacatctc
    25681 ctgattgacg acgagacagc accgattgtc cggaaaattt tccagtgggc ggtggacggt
    25741 catgggatta actttatatc cagagaactg gaacgccagc agattccctg cccgtcatgg
    25801 tggcaccgga aacggggact ccgcacctat tacacaaaat gggaaaagaa agacccggaa
    25861 aatgggaaat atgtatggga tgaatcctat ctgaaatccc tgctgatgaa tcccgtttac
    25921 tgtggtgaca tggcttccca gaaaaggtac taccgtttca agattggcaa tcagggagat
    25981 aaagctccgg aggactggat cacagtcagg gatacccacg aagcaatcat tccgcaggat
    26041 gtatttgatc tggtacaggc aaagatgaaa ggcaggaaac gccagacaga acagggagaa
    26101 tacagcatgt ttgccggatt actccgctgt gcagagtgtg gcggtgcgtt gacgttgaag
    26161 aagacgcaca ccaaagacca gcatgaagtt tatacctgtt ccacttatat ccataagggg
    26221 aaagcacatt gtacccagca ccgggtggac gctgatgacc tgtatgatgc agtccttacc
    26281 agaatccagg aatgtgcaaa agcagttacc ggagacggaa cagaactgga aaaccgggta
    26341 aaggagttat gcgaggaaga tacacaggga cacagggaaa gtctggagaa gctggtatca
    26401 aagcagaaag accgcctgga aacgttagac cggctgattg caaaactgta tgatgacctt
    26461 atcaatgaca agattacaga atctgttttt gataagatgc tggagaagac acagaaggag
    26521 cagacagata tcaaaaagga acttttccag aatgagagtg tgctgaatac ggaagaaaaa
    26581 ctggatgcac agtcccagca gtggattgac gatattagcg agtatgcgga catcaaagaa
    26641 ctggatgcca atcttctgaa ccggctgatc agtaaaatcg ttatctcaga accgcaggag
    26701 aaagacggaa cagaaaatta ctgccgtact cctgaaacgg tcacaatgga aattcatttc
    26761 aacttaaaac caataccgga gcttggaacc atcgaaagag gttctggctc ccataaataa
    26821 gaaaaatcta tcaaaattta atctatttta atcttttcaa ggctcatttc gagcaaatat
    26881 tttctcataa aagacataaa acagcttgtc gctggcggcg gcgtggccct gatcggtgtc
    26941 actctggttc ccctgctgtc cgggctgctg ggctgatcct ggcctttgtc cctgtccaaa
    27001 cccttaacta atcgcctccg cgtcctcccg gccagaccgg gagggcgcgg gaaaggaggt
    27061 gctacccgtg attagtgaaa tcattgaaga atggataaaa ggcatcctca tggacggtat
    27121 cacgggcaac ctgtccgggc tgttcgatac ggtaaatgcc aaagtcggag aaatcgccgc
    27181 cgacgtggga cgaaccccgc aggcatggaa cagcggtatt ttcaatatgc tgcacagcct
    27241 gtcggaaacc gtgatcgtcc ccatagccgg ggccatactc gcccttgtca tgtgctatga
    27301 gcttatccag atgataacgg aaaagaacaa tatgcacgac tttgacagct ccatgttctt
    27361 ccgctggata ttcaaatccg cgtttgcaat cctgatcgta acgaacacat ggaacatcgt
    27421 catgggcgta ttcgacgcca cccaaagcgt cgtcaatcaa agcgccggtg tgattatcgg
    27481 gcagacatcc attgatttta acaccctgat accagacctt gaaagccaat tggaggctat
    27541 gagcgtgggc gctcttttgg ggctgtggtt ccaaaccctt gttgtgggcc tcaccatgaa
    27601 tatcctttcc atctgcattt tccttgtgac ctatggccgc atggtggaaa tctatgttgt
    27661 cacgtcgctg gggcctatcc cccttgccac aatgggcaac gcggaatggc gcggcatggg
    27721 ccaagggtat ctgaaatccc tgctggcgct gggcttccaa gcctttttaa tcatggttgt
    27781 ggtgggtatc tacgcggtgc tgatacagaa tatcggttcc gcttccgaca tatccggcgc
    27841 aatctggggc tgtatgggct atacggtgct gctgtgcttc tgcctgttca agaccggcag
    27901 tatatcaaag gccgtattcg gggcgcattg atcccaaaat ccaagcatac acaggaggaa
    27961 cttgtttatt tcaacgtaca acatcattta cgctgacccg ccgtggcggt atgactgcaa
    28021 aaacgtacag ggcgcagcgg aaaatcacta ttccaccatg agcattgacg agctgtgcgc
    28081 cctgccggtg gaacggctgg cgtccaaaga ctgcctgctg tttttgtggg ccacattccc
    28141 catgctgccg gaagctctgc ggttaatcaa agcgtggggc ttttcattca agaccgtggc
    28201 ttttgtatgg ctgaaacaga accgtaaaag ccttacatgg ttttacgggc tgggccgctg
    28261 gacaagggga aacgcggaaa tctgcctgct ggcaacacac ggcaagccga agcgtcgttc
    28321 tgcctctgtc catcagttta ttatcagccc gattgaacaa cacagcaaaa agccggatgt
    28381 gacccgtgaa aaaatcgtag agctggcggg cgatttgccc cgcgtggagc tgtttgccag
    28441 acagaaaacc cccggctggg acgtgtgggg gaacgaggtg gaatgtgacc ttatcctgac
    28501 gtgaaaggag gtgccaaatg gcttatgtaa ccgtccccaa ggacttaacc catgtgaaaa
    28561 gcaaggttgt gtttgggctt accaaacggc aactgatctg tttttccggg gcgctggtga
    28621 tcggcgtccc cttatatttt ctcacccgtg actatcttac caacagcgcg gcggccctgc
    28681 tcatggtatt tgccatgctg ccgggctttt tgcttgccat gtacgagcgt cacggccagc
    28741 cccttgaagt ggtgattatg cagatcatcc agtgctgctt catccagcca aaggagcgtc
    28801 cctatcagac cgacaacacc tacaccgccc ttgtgcggca ataccagatg gaacaggagg
    28861 taaaggccat tgtccaaaaa ggaaatccca agagaaaccg aaaaaacaaa gctgacccgc
    28921 gcccagcgaa aagaaattga cgcggtaatc cgtaaatata agggcgacgg caggccccac
    28981 acggcgcagc agagcatccc ctacgaggtc atgtacccgg acggcgtgtg ccgggtatcg
    29041 cccggcgtct tttccaagtg cattgagttt gcggacatca gctatcagct tgcccagccg
    29101 gacacgcaga cggctatctt tgaaaaactg tgcgacctgt ataactatgt ggacgcctcc
    29161 attcatatcc agttttcttt cctcaatcgg aaagttgacc cggtgcagta cgccaagagc
    29221 tttgagatcg cgccgcaggg ggacgacttc gacgacatcc gggccgagta caccggcatt
    29281 ctgcaaaagc agcttgcaaa cggcaacaac ggcatggtaa agaccaaata cctgacattc
    29341 accatcgagg ccgaaagcgt gaaagcggcg cgagccagat tgaagcgcat cggctttgac
    29401 ctgttaggct atttcaagag catgggggcc gtagcccatg tgatggacgg ctgggaacgg
    29461 ctgaacctgc tgcatggcgt gtaccacccg gacggcgaga tattcaattt tgactggaag
    29521 tggctggccc cgtccggcct ctccaccaag gattttattg ccccgtcctc cctgtgcttc
    29581 ggcaatgcaa agacattcgg catgggcggc aaatacggcg cggtaagttt cctgcaaatc
    29641 ctgtccccgg agctgtcgga cgatatgctg gccgacttcc tcaatacgga aagcggtgtt
    29701 ttagtcaatc tccatgtgca ggccattgaa cagaccaagg cgatcaagac catcaagcgc
    29761 aaaattaccg accttgacgc tatgaagatt gcggagcaga aaaaggccgt ccgcagcggt
    29821 tacgatatgg acattcttcc cagcgacctt gccacctacg gcgaggacgc caagaagctg
    29881 ctgacaaagt tgcagacccg caatgaacgg ctgttccagt tgacgttcct tgtcctcaac
    29941 gtggccgaca ccaagcaaaa gctgaataac gacgtgttcc aagccgcagg cgtggcccaa
    30001 aagcataact gtccccttgt ccggctggac taccagcagg aacagggcct tgcaagcagc
    30061 ctgccgctgg gcgtcaatca gatcaagata cagcgcagcc ttacgacttc cagcgtggcc
    30121 gtgttcgtcc cctttgtgac gcaggagctt tttcagggcg gcgcggccat gtattacggc
    30181 atcaacgcca agtcccgcaa catgattatg ctggacagga agcaggcccg ttgccccaat
    30241 gccttgaagc tcggcacccc cggcagcggc aaatccatga gctgtaaatc cgagatcgtc
    30301 agtgtgttct taacgacgcc tgacgatatt tttatttcag acccggaggc cgaatattac
    30361 ccccttgtca aacggctcca cggacaggta atccggctgt cgcctaccag caaggacttt
    30421 gtgaacccgc tggacatcaa cctgaattat tcggaggacg ataacccgct ggccctgaaa
    30481 tccgactttg tgctgtcgtt ctgtgagctt gtcatgggcg gcaagaacgg gctggaagcc
    30541 attgaaaaga ccgtgataga ccgggctgtg cgcgtgatct accggcccta tctggcagac
    30601 ccccgcccgg agaatatgcc gatcctgtcc gacctccaca aagcgttgtt agatcagcat
    30661 gtcccggagg ctgaccgggt ggcgcaggcc cttgacctgt atgtgtccgg ctccctgaac
    30721 gtatttaacc accgtaccaa cgtggacatc ggcaaccggc ttgtgtcctt tgacattaag
    30781 gagctgggca agcagctcaa aaagctgggg atgctcattg tccaagacca gatatggggg
    30841 cgcgtcacgg ccaaccgaag ccaaggaaag gccacatggt attttgccga cgagttccat
    30901 ttgcttttga aagaggaaca gacggcggcc tattctgcgg agatttggaa gcggtttagg
    30961 aaatggggcg gcattccaac aggtgccacc cagaacgtca aagacctgct ttcctccccg
    31021 gaaattgaaa atatccttga aaacagcgac ttcattacgc tgctcaatca ggcgtccggg
    31081 gatagaaaaa tccttgcgga acgcttgaat ttgtccaccg aacagcagaa gtacatcgac
    31141 aattccgaac ccggcgaggg ccttttgatc tttgaaaacg tggtgctgcc ctttaccaac
    31201 cccatcccgc acaacaccca gctttacaag attatgacga cccgcttaaa cgaggtggcg
    31261 ggagtatgac ccggcgacgc cacaaaggag gtgaaccacc atgaaagagc tgaaagccaa
    31321 ggcgaaagtg acgcaggcca tgacccgcga cgggctgacg ctggaaaatc aggccaccgg
    31381 cgaggtggag aacatttcca gccgggaggc cgaacaggat ttgtccgcgc cggggccggg
    31441 cggcaccgcc gataagctgc tggaacgggc cgacgccatc catgacggcc ataaggccaa
    31501 gaaagccgcg aagcaggccg gggctgtggc cgccgagggg gacgctgccc tgcaccggcc
    31561 atccgcccgg ctccaactgt cggaggaaga acgggccgac ccggatttac agccctatat
    31621 ccgaaaatcg gacaaacggg ccgataagct ggataaggcc cgcgccgcca tccccaagaa
    31681 gcgcgtcccg gtcaaggaaa ccgtctttga cgaggcaagc ggcaaagcaa agaccgtcct
    31741 gcactttgac aaacaggaca aggggcctcc ctccctgaaa cccaatccgg caggccgtcc
    31801 tgtttccgag gtttttctat tcggccacgg gaaaatccat gaagtcgagc atgaaaacgt
    31861 gggtgtggag ggcggccata agggggagga acttgccgag cggcaggccg gaaaagccat
    31921 ccgcagcacc atccggcacc acaggctgaa accctactgg gacgcggaaa aggccgagcg
    31981 caggctattg tctgccaacg cggagtattt ctaccagaaa tctttgcggg ataacccgga
    32041 gatcgcacag gcggccagca accccatttc ccggttctgg cagaaacagc agatcaagcg
    32101 ccagtacgcc aaggccgccc ggacagcggg ccagaccgca ggcgcggcgg cacagggcgc
    32161 ggccaagaca gccggggcct ccacgaaagc ggccaaggcc gcagccaaag aaagtgaacg
    32221 ggcggcctcc tttgtggccc gccactggaa aggggcgctg gccgtccttg tgttggggct
    32281 tttgatgctg cttatcatcg gcggcttaca gtcctgcacc gccatgttcg gcagcgccgg
    32341 aagcggcatc acggcgtcct cttacttttc cgaggatagc gatatgttgg ccgccgagga
    32401 cgcctacgcg gagctggaag ccgagctgca agactatctg gacaactacg aggccaccca
    32461 tgactatgac gaataccatt ttgaccttga cgagatcagc catgacccct atgtgctgat
    32521 ttccattctg tcggcgctcc atgacgggat atttaccatc ggggaggtac agggtgattt
    32581 gtccatgctc tttgacaagc aatatatcct gacagaaaac gtcgtggtag aaacccgtta
    32641 ccgcaccgaa accgatacat ggacggacgc ggagggcaac acccatacag acacctacca
    32701 agtgccgtat gactattaca tctgctacgt cacgctggaa aacttcgacc tgtcccatgt
    32761 gccagtctat atcatggacg aggaaacgct aagtttctat tccctgtata tgtccacgct
    32821 aggaaaccgg cctgatctgt tttccggcag ccagtacccc aacgcctcca ccctgacgcc
    32881 gccgacctac tacgacgtac ccccggaggc tttggaggat gccacctttg ccgccatgct
    32941 caaagaggcg gaaaaatata tcggttatcc ctatgtgtgg ggcggcagct ctcccagcac
    33001 cagctttgat tgcagcggct tcatttcgtg ggtaatcaat catagtggat ggaacgtggg
    33061 acggctgggg gcgcagggcc tatataacat ctgcacccct gtttccagtt cgcaggccaa
    33121 gcccggcgac cttgtatttt tcaagggaac ctacgacacc cccggcgtgt cccacgtcgg
    33181 cctgtatgtg ggcaacaatg tgatgctgca ttgcgggaac ccgatttcct acacgaacct
    33241 tacttcaaac tactggcaat cccattttta cagttacggg cgtttacctt aacgccagaa
    33301 aggagctttc aattatggca accaaacttg accgcatcga aaaggacatc cagaaaacca
    33361 agtctaagat cgcggagttt cagaaacagc ttcgggagct ggaaacgcag aaaaccgaac
    33421 aggagaattt gcagatcatc cagcttgtgc gcggcatgaa catgaagccg gaggaatttg
    33481 ccgcgttctt acgcagcggc gcaatgcagg ccgcccctgc cgcaacccct taccatgaac
    33541 aggaggacaa tgcccatgaa gaataagacc agacggattt cccgtattct ccccgcactt
    33601 ctggccgtga tgctctgcgt cacggccttt cccgttaccg cccttgcagg cggcaatgac
    33661 ccggcaccgg ccccgctgcc ggaggctaca accgaacccg ctacgggcgg tttggagcct
    33721 gaaacagacg aaaccggcac cgccacggac gggacagaaa ccgaggccga aaaagatacc
    33781 ggccctctga ccggcaagga catcaccgac ctgttttccg cgctgttcgg ttccaaggtg
    33841 agcattgccg ccacggacga cggcatccag atcaccaccg gcaccgacaa aaaagaacct
    33901 gaacagaccg gcaccgtcac caccaacggc ggcaggctga atgtccgcac cggcgcgggg
    33961 cttgacaaga ccgcgttcac ccagcttccc aatgggacga cggttgaggt agtcggcaca
    34021 gacggcgact ggattaaaat tctcttgccg gagcgcatcg gctatgtcca ttccgactat
    34081 atgaccgtca gcgaaaagga agtagcggct accggcgagg gcggtttttc cctctccatt
    34141 gacccggagg aaattgcttc tctgctggaa ctgttcaatg gcggcggggc cagcgccgcc
    34201 ctgtccccgg acgggaacct gtcactgatt gacgacatcg gcagcgccgc gaaaaatggc
    34261 aagcagttta tcaccgtgga aaccaaaaac ggcaatgtgt tttatctgat tattgaccgg
    34321 gacgacgagg gaaaagaaac tgtccatttc ttaaatcagg tggatgaagc cgatctgctg
    34381 acgctgatgg aggacgggga aaccgtcacc cccgctgctg tctgctcctg taccacgaaa
    34441 tgcaaggccg gggctgtgaa cacgaattgc ccggtatgta aaaccaacct gaccgagtgc
    34501 agcggcccgg agccgcagga accccagccg gaggaacccg aagccccgga ggaagaaccc
    34561 aagggcggcg cgggcggcct gatcgtgttc ctgctggtgg cgctggcggg cggcggtgcg
    34621 gccctttact atttcaagtt caaaaagccc aaggctgaaa ccaccggcag cgacgatttg
    34681 ggcgagtatg attttgggga ggacgaggat ttggaggacg aggaacccga aaccgagttt
    34741 gacccggaag ctgattttgt gagtgaacag gaggacgagg aatgacatat aaacttgtaa
    34801 tcgcggaaaa acccagcgtg gcccgcagca ttgcgggcgt gatcggggcc acggagaaac
    34861 acgacggcta tttggagggg aacggctatc tggtgagctg gtgcatcgga catctggtgt
    34921 cctttgccga cgccggacgc tatgacgagc gtttcaaaaa atggcgctat gaggatttgc
    34981 ccattctccc ggaaacatgg cagtacatcg tccccgacga caagaaacag cagtttgacg
    35041 ttctctgttc gctgatgcag cgccccgacg tgaccggcct tgtgtgcgcc accgacgccg
    35101 ggcgcgaggg ggaattgatt ttccgtttcg tctaccagat ggcgggatgc caaaagccct
    35161 ttgagcgcct ttggatttcc tctatggagg acagtgctat taaggacggc tttgcccacc
    35221 tgaaaccggg cggcgactat gatccgctgt atcaatcggc cctgtgccgg gccaaggccg
    35281 actggctgat cggcattaac gccacccggc ttttttccgt cctgtaccac aagaacctga
    35341 ctgtgggccg cgtccagacg ccgaccttaa aaatgctggt ggacagggac gccaagatta
    35401 ccgatttcaa gaaagagaaa tatcacatcg tccatatcac ggcgggcggc gcggacgcgg
    35461 taagcagccg cttttccgac gctgccgagg caaacaccgt caaggcggct tgtgcaaaag
    35521 cgcaggccgt ttgcgtttcc gtgacgcggg agaaaaagac ggaacagccg cccaagctct
    35581 atgaccttac caccttgcag cgggaggcta accggctttt cggctatacc gccaagcaga
    35641 cccttgacta tgcccaggca ctgtatctaa agaagctgct gacctatccg agaacggata
    35701 gtcaatatct taccgacgat atgctgcccg ccgtggaaag ccttgtgtcc ggcctgtggg
    35761 agctggtgcc gtttgccaag ggcctgaacc tttccccgca gtttgaccgc atcctgaaca
    35821 gcaaaaaggt gtccgaccac cacgccatta tccccaccgc tgaatttgtg aagcagggct
    35881 ttggcactct ggccgagagt gaaaaaaagc tgctgtccct gatctgctgc aagctcttat
    35941 gtgccgtggc cgggccgcat atctatgagg ccgtcaccgc cacatttacc tgtgccgggc
    36001 aggagtttac cgccaagggc aagaccgtcc tttcccccgg ctggaaagag attgaacgcc
    36061 ggttccgttc ttccttaaag accgacgccg acgaggacgc cgaggcggtg cgggagctgc
    36121 cggagctttg cgagggccag acctttgcag acgtggccgc ctctgtcacc gagcatttca
    36181 ccacgccgcc caagccctac accgaagata cgcttttgtc cgctatggaa cgggccgggg
    36241 ccgaggatat gccggaggat gccgagcgca agggattagg cacaccggcc acccgcgccg
    36301 ccattctgga aaagctggtg cagatgggct ttgtccagag aaaaggcaag cagctcattc
    36361 ccaccaagga cggcatcaat ctggctgtgg tgctgccgga ggcgcttact tctccccagc
    36421 ttactgccga atgggaaaac cggctgaccg agatcgccaa ggggcaggcc gatcctgacg
    36481 aatttatgga cggcatcgag acgcaggcgc gggagctggt gcagacctat tcctgtatca
    36541 gtgaggaaaa gcagaaattg tttcaggccg agcgtgttcc tatcgggacg tgtccccgct
    36601 gtggtgaggc tgtctatgag ggcaagaaaa actactactg cggaaaccgg gcctgccagt
    36661 tcgtcatgtg gaagaatgac cgattttttg aggaacgcaa gaaagcgttc accccgaaga
    36721 tcgccgctgc gctgctcaaa accggcaaag tcaaggtaaa gggcctctat tccgtaaaga
    36781 ccggcaaaac ctacgacggg acggtgcttt tggctgatac cgggggcaag tacgtcaact
    36841 accgcattga acacaggagc taaaggacag taacacagca agcataggga gtgtggctac
    36901 actcccggta aaatcccccg gccgcgtccc tgccgggccg gggatttttg cttgttgggg
    36961 aagtccccaa ccccctattt tttgaaacag aacagagaaa ggagtgaccg cgtgaacgaa
    37021 gtattttcta tccaagtcca taaccggcag tttttggaac agggcaaaca aggcgtctgg
    37081 ctggatttgc ccaccacaac cgaaacgctg caagccgccc tgcgggatgt tggcattact
    37141 gccgacaatc cgcaggattt ttttatcaac gggtattcct gcccggagga cagacacctt
    37201 gccattccct atgacatggt gctggccgcc gacgtggacg agctgaattt ccttgccgcc
    37261 cggctgggcc agcttgacgc cgccgagatc gcagagctga acgccgccct gcaaaatccc
    37321 cgtggcggct ttgagaatat cggccagatc atcgactaca ccgaaaatgt ggattactat
    37381 gtgcatcttc ccgacgttag gggggatgga cagttgggcg actactacct gaaccgttcc
    37441 ggcatggtgg atatgccgga ggaatggaaa gccgggattg atacggcgca gtttggacgc
    37501 cacattgccc agcaggaaca aggcgttttt acccagtacg gctatctggt gagaagcggc
    37561 gacgaatggc agcgcgtcca tgagggccag ccggtgccgg aggaataccg ggtgatgcac
    37621 tacccccagc cggaagctga ccgggaggaa ccgcaggccc agcagccgga ggcatccgaa
    37681 agccccgaca aagcgccgcc cccggttatc ccgatcatgc tggacggcat gaacagcaaa
    37741 gaacgcatga aagtgattac tgaccgtctg gaaaccggcg tacaggagct ttttgaaagt
    37801 agccgctata tggcttactt atccgctatg tccaagatgc acgattacag tctgaacaat
    37861 acgctgctga tcgccatgca gggcggttcg attgtggcgg gctataacag gtggcaggat
    37921 gaatttgacc gccatgtgaa gcggggcgag aaaggcatta agatactggc cccggccccg
    37981 ttcaaggtta agcagcagat ggagaaaatc gacccggcca cccagcagcc cgtgatcggc
    38041 gcggacggca agcccgtgac cgaagaacag gaagtgacgg ttccggcctt taaggtggtg
    38101 acagtctttg acatcagcca gaccgagggc aaggattttc ccatgcccta tatcagtgaa
    38161 cttaccggcg acgtgcagca gtatcaggat ttctttgccg cgctggaacg gacatcaccc
    38221 tatcccataa gcattgagcc gatttcagac ggggccaagg gccgctgctt ctacgcaaaa
    38281 cagcgcattg ccatcaacga gggcatgagc gaattgcaga ccctaaagac cgccatccat
    38341 gagattgccc atgccaagct ccacgccatt gaccgggagg cccccgccga ggaacaggcg
    38401 gcccggccag accgcagcac ccgcgaggta caggccgaaa gcgtggccta taccgtctgc
    38461 cagtattacg ggctggacac gtcggactat tcttttggct atgtggccga ttggagcagc
    38521 ggcagggagt tatccgagct gaaagcctcc cttgaaacca tccgggccac ggccagcgaa
    38581 ttgataaccg agatcgacgg ccattttgcc cagcttcagc aggagcgcga ggcccggcag
    38641 gaacaggagg ccgtcacggt tgacgttacc ccggaggcag cggccccggc gcaggccgag
    38701 gaagaacagg ccccggccct tgatccggca gccgagccgc ttgtgactgt gctatggagc
    38761 gaaagccccc agctaaagga cggccagcaa atgccgttgc atgaggccga cgcgctgttt
    38821 gcccgtctgg atgcagcata tccggcagag gcaggctatg acaaaaccgc gttccgcatt
    38881 gactgtatct ttcagggcga gccggacagc tacgaggggc ggcaggattt tggagatagg
    38941 gacggttccc tgatcgaaca tatccagagc taccaccaat tttacgcgca ggacgaaaac
    39001 tggaaaaagc atgtaatcgc tacaaccggc atagagggct gggagcagga caaggcccat
    39061 cgggatatgc tgttaggcga gtttgtcccc tatatgaagc tgcattgcag ccttgcccaa
    39121 atggaacaga cggcgcaggg ccgcttacag tccggggaaa ccctgacgcc ggaggaaacg
    39181 gcctatttgg gggcgcttct ctcttatgtg caggagtgcc gccccatgct caatcagggc
    39241 cagtatcagt tgccggagcc gccgaaactg gctgacttcg accagagctt gcaggactac
    39301 aaagcacagg tacaggccga gatcgcgcag gaggccgccg cagccggtat gaccgtggag
    39361 gaatacgcgg cggctggcta tgaagcaccc caacaggaca ccttttccat ctaccagctt
    39421 cgaggcgggg aggaaacccg cgactatcgg tttgagccat acgagcgttt acaggccgca
    39481 gggctggccg tggacagtgc caactatgac cttgtttata cggcaccgct ggataaaagc
    39541 acgtctttgg aggatattta ccgcacattc aacatcgacc acccggcaga ttttagggga
    39601 cacagccttt ccgtttccga cgtggtggtg ctgcaccaga atggacagga caccgcccac
    39661 tatgtagaca gcttcggcta tatggaagtg ccggagtttt tgcgggaaaa tcccctgcgt
    39721 accgccgaac tgtctacgga gcagaacgaa aacatgattg acggcattat caacaatacg
    39781 ccgcccgaac cgcagccgga ggaacccgaa cagctatctt ttgaggatgt ggcagttgct
    39841 tccggccaga cacaggagga acccccggag gctgcgcccc cgcaaacggc tgtccgctat
    39901 tatcccatca atgagggagc cgcccgccgc gccaaggaag ccatgagctt ttctgactac
    39961 cggcccggca gcgccacggc ggaataccgg cactatgtcg atcaggccgt ggaaattgcg
    40021 gagcgccaga aaaagcgtgt ggagccagag tatcacgaaa agatagacag cctccttgac
    40081 acctacgccc gcaagctggc cgagaacatg aataagggct atgagattac cgcccgcgtc
    40141 ccctctgtcc tgattgccgg gccgtccaat ttcccggtac gggcgaaaga aaagcagaac
    40201 gccgccagtg accgcaacat ggaggaattt cagtacattc agggcttgct tgacaaaatc
    40261 cgcagcaccg gcatgggtgg tatcagcgcc gacgatccgc aggccgtttc caagctggaa
    40321 aagaaactgg aaaagctgga agcgtcgcag gagttgatga aggccgtcaa tgcctactac
    40381 cgcaagcatg ggacgctgga cggatgcccc catctgaccg aaagaggcat tgaaaacctg
    40441 aaagcggata tggcgtcggg ctggcactat gagaaaaaac cgttccagtc ctggcagctc
    40501 tccaacaata acgccgagat acgccggtta aagggccgca ttgaggaatt gacccgccaa
    40561 aaggaggcgg cctatgtggg atgggagttt gacggcggca ctgtggagat caaccgggtg
    40621 gccaaccgcc tgcaaatctt ctttgagggg aagccggatg ctgctgtacg cgacgagctg
    40681 aaaagcaacg gtttccgctg gtcgcccaag gccggggcat ggcagcggca gttaaacgac
    40741 accacaatcc gcgtcgctga ccgtatcaag tgcatccagc ccctttccgg cgaaaagccc
    40801 agcgccttac agcttgcggc ccgcagggag aaagaaaagc cctctatccg ggcgcagctc
    40861 aacgcggcca agaccgaaca ggccaagcag ccaccggcgc gggaaaaatc aaagggattg
    40921 gaggtagagt aaatgcagaa gtttgaaaat gtggacgttc tggccgcgct ggaacagatc
    40981 atgcggcaga acaccgcgtt ttatcaaagt gactttggca ttgacaaaga cattatcagg
    41041 cgggatgccg ccagcgatca ggccgcagac aagaccctgc tctggatgtc ccggccctcc
    41101 gggacgtact gtttccggga acgtgatgtt ttcctgaaag acacccgaca gtacaacaca
    41161 tggaaatttt acggggagca gacccgtgac aagatactgg cctacgcggt ggagctgacc
    41221 ggcacacagg gcggcactat ccggggcaat ctctatgagc tggactatcc gcagcatttc
    41281 cggcacgtca ttgaaaccgc ccagccggtg agtgtgaacc ggctctttta tgagcatggc
    41341 acccgtgaca tcccggagag ccagcgtttt gacggaagcc ctgaccgggt attgggaaac
    41401 tttttgtact atgaggccca gccccatgac ccggctgtgc tgcaggaggc gctacggacg
    41461 gagcagcaca gccgggggcg ggccgtcccc ggcgatttca aagtccacgt caccgctttg
    41521 catgacagcc ggattgaaac cgaggcccgg cgtattgtgg acagcctgaa agatctgtcc
    41581 gcgcctaaca gccccaataa aacgcatttc atggtggaga tctccccgca ctttgcggcg
    41641 attgcttcca ccaaggataa tgaccggctc tttgccatgc tgccctataa atccctgtgt
    41701 tttaccggga tgaaaggcag gcatggcgtg tatgccgtga ttaaccggga cgagcgccgg
    41761 gatgtgagta ttcgcaggcc ccggccctct atccgcaagc agctatccga cagcaaacag
    41821 gccgccagcc caaaaaaggc ggcggccaga accaaaaaga atgaattgga ggtataagga
    41881 atgatgcact ttaccgtgga ggaagaaaac ctgatctgta tgtaccacaa tgcagaccgg
    41941 cgcagaacga taaacaggct caccgccgtc atgccggtta tggatgggga tatgcgggcg
    42001 caggcccagc agaccctttc caagctggaa cgcatgacgg atgccgacta tgacggccag
    42061 aagttccact tcacagacga ataacgaaca gcagacgccg ggcgcggcgg ccatgtggtc
    42121 gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc atttagaacc cggtattgtc
    42181 ggggatagga tgaagtattc gcttgcccct gcaaacatgc gggagaagcc ttgcagcggg
    42241 cggttttcaa tcccttaaat gcgtaggttg cagctataat tagacccctg aaacagatat
    42301 agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt gatagccgca aagcggcgta
    42361 agagggtgca gccctcttgc cgtctatctc tgttccagcg ggaagctcgg caatgcttcc
    42421 agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa tgactttctt ctttttgccg
    42481 tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt agtcagcata atgggccaac
    42541 actttttccg tggcccacct ctcaccggca acggcggctt tgatcgtttc atagtccagc
    42601 agcttttctt gtccgtccat cctgataccc ccgtcagact gattttttct atgataccat
    42661 attcggaagc atggaaccag agaaaacggc gcttttctgc tggtaaattc tacgcattta
    42721 gaacagcact tcggcgggga taggataaaa cacccacata ccccgctgac catgccggaa
    42781 aagccttgaa atataagggc ttttacagct ctaagtgcgt aggtttccag tatcttttcc
    42841 gctggcgctc ggcctctttg cggcggcgca tccgtttgga gcaggccgcg cagtatttgg
    42901 cccggttaga cttcggcgtg aacgtggccc cgcactcaca gcagcgtttc acttcgtccc
    42961 ggtctttggt gatctcggca tacagggccg catccagcgg gaggacagcg gccttaaacc
    43021 agcggcacag aagcgaatag gaaatgctct gtacgcaaat acattcttcg ccgtcgtcca
    43081 gcaacaggca gttgccgtca caatagttgc agcactcatg cgtcagcttc cgggccttgc
    43141 gatattgagg gtaattcatt cgtggaatac tcatagctct tgcgtccatt cctttcccgg
    43201 cgtcatacgc aaaatgccgt ccacattctg cttgatgatg ccgtattcct gcatctgctt
    43261 tttcgcttcg ccgtacttct catacagccg ttccttttcc tcggccaatc gcttgtactc
    43321 ggctttcagg gcgtacagat cggggagctt tttcacaccc tgtcccttta gggcctttgc
    43381 cgcagcttca tacaaaatca acgtgctttc atgctcacgc cggaattttg ccttatccgc
    43441 tgcattgcgg tattccatag ccacgggcca gagctgcttg taggtagaaa tattctttat
    43501 cagcaccgcc atatccgcaa gccggtgttc catagctttg agggtagcgg cggcctcgtc
    43561 gctggcggcg gtgatctcgg ccactttgct ttccaaatcc gggtaggtgt ctatcccatg
    43621 ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa ttgtggagct tcgcccacct
    43681 ctcatagcct gccgactgct gggccttaat gttgttttcc agatcaatcc gcaaagaaat
    43741 ccccttgcgc tctttgggcg ctctggtgat aacgcggcct ttgatacgct ctttgatggc
    43801 ttcctcggtg taggcttccc ccaatgtctt gcagcgggta aagcgttcct gtccgggagc
    43861 gcggaaagaa ataaactttc ccggcttgat ctcatagccc tgcgcctgca tgagccgcag
    43921 gaaatcatca aaatcttttg cctgcgggat agcggcgtca atggctgctt tgagcttcgc
    43981 tttccagctc gtccctttcc gctgggcgtc ccactcggca tagctcttgc ccttactctg
    44041 gccgggcttc accacggaca atccgttttc cttgcacagc ctgtcgctgg tgcgccggat
    44101 ataggcatag ctctgtttgt tggaaatgta cttgcgctga tttaccatat ccacggcaca
    44161 gaaaataatg tgattgtgga cgtggccctt atcaatatgg gtagtcagca catagggata
    44221 tttcccttgc agcacttcct cggcaagctg cttcccgatc tcatgggcct gttccggcgt
    44281 ggcttcgccg ggggcaaagg attgtatcag gtggtggccc agattattgc ccttttggta
    44341 tgcctgcgcc agcagcattt caaattcaat gtccgctgtt tcataagagc aggcaaagga
    44401 ggccaccagc agcttgccgt cagttttgtc cgggttctcg atgtagtcaa gggctttttt
    44461 cagcgttgac ttgataggat gtatctttgt aactgccata tctgcgccag cgcccccttt
    44521 atatcctcga tgtcctgtgc gtacaccggc cccataccgt tcagcctctt tgcaatctga
    44581 ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg cccggatgtc gctggtgtcc
    44641 atgttgacga tatagccgtc aatgagcatc ttccgggcgt aggctgaaaa gttcttcgta
    44701 tgcagcagcg ccattttctg ttcaatcata gcccgttcct ccggcgtcac agggacgcgc
    44761 agcacgattt ttcgctttct gtttgccatg cttcaccgtc ctttccattc aggggtttgg
    44821 ggatttcccc aacaagcaaa acggacgcgg cccggcaggg acggggcagg ccgtttttcc
    44881 gggagtggct acactcccta tgcttgctac gttactgttt ctccactaac aagacgtttc
    44941 caaagcccca aactacccgc aaaatagccc tctggaaaga aaaaagtgca aaaaaataga
    45001 agctacgctg tgcagctccc gctaaaagta aaacagagga tgccagttac acatcctccg
    45061 tttcattctt cttcggcttt tcaattagta tggcgctgtc aatcgcgccc tcgatgatcg
    45121 gcaggtattc ttcggggcag agtttcagct tgtggctgac cctttgccgc agttcgcttt
    45181 cctcccgcat gatctccggg ttaaagtagc gttccacagg aagcccgcag gctttaataa
    45241 gctgtatgac aaccggcaga ctgggaatgg ccccgtcgtt ttcgatgttg gcaagatagc
    45301 gccagtcaat atttattttc tctgccattg ctttgcgcga aacgcgcttt gcttgccggg
    45361 ccgctttcac atccgcgccg aaagtttcaa agccgggaca atcttcaata ttcgccatat
    45421 cgcacctccc acttacattg tatatttcat actctgcccg tggaatgttg gtatatgcac
    45481 ctttcttaaa gtttatattt cacattttgt ttcttttatt cttcgagcaa aagaattata
    45541 atgtaccctg tggaggtgta ctatggacta catgacgttg aaagaggcag gcgagaaatg
    45601 gggtgtgccc ccacgatgga taaactacta ttgtgctgcg ggacgcatcc ccggcgctgt
    45661 aaagatggcg acgatctggc tcattcccaa aactgcggaa aagccgattg acaggcgtac
    45721 aaagaaagga aaggcggtta ctgaatagta taatgaaacc ggcgcatttt ggatatggtt
    45781 ttgaagagag agaaagcatg gtattttgtc acggaaaaac tataagatga tagatggcag
    45841 gctgctccag accgataaaa agtattctca tctgaaacag aaagaaaaga tttacacatg
    45901 gatgtatgaa gaagcgaaac tttatcatga cagaaatgga aaatgcccga aacaaagacg
    45961 aggaagctgc tgctgtatca gccgtatatg gtagaattga aaaggccggc atctggattc
    46021 tttatggtga agtctccaga cattggataa ctgctggtct aatgcgcctt tgagtatggg
    46081 gtgattgatt ttaataccgg ggaggtacgg ctttttgagg aaaatattct gggatttaca
    46141 tttggcgatt attacatttc ctgtaactgc aaggacacgg atgatgaaga tatgaggaat
    46201 atagacttgc tgattttcta ctgtcttgaa cggtattagg actgaatctg ctgcgcggag
    46261 tgcggcaaga gcgaccggag ggagtacaca caggccctgc ctgtgtgaaa agccataccg
    46321 caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga ggcttggggt gtccccaaca
    46381 agcaaaacga gaacgccgca ggcgggcaag tggtggatca gaatatccgt aaaggatatt
    46441 ctgatccatt gcttgccagt attatcggga tgttttgaca accgctgtaa tcgaaacatc
    46501 ccaaacatta agaaggtgtt aattattatt atcttgattt aattcgttta aaccaataat
    46561 atagatacat acaccatgaa aagaagcgag tatcccaatg acggatgatt aaaatccgaa
    46621 aagaaatcgc tttgaaacta cagggaaact acagactgtt atgttatctt cttaaaatgg
    46681 agggattttt atgtcgatac gaattctact tgtcgaggat gatgatcata tctgtaatac
    46741 agtaagggcg tttctggctg aggcaggata tcaggtggat gcctgcacag atggaaatga
    46801 ggcatacacc aagttttacg aaaacactta tcaactggtt attcttgata ttatgctgcc
    46861 cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg aaaaatgata ctcccattct
    46921 gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg gcgtttgatg cagaggcaga
    46981 cgactatgta acaaagccat tcaagatgca gattttacta aagcgggtgg aagccctgct
    47041 gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc aggctgacac ttctgccgga
    47101 ggatttcacg gtactttgtg acggtacaga gctgcccctg acacgaaaag aatttgaaat
    47161 ccttttgctg ctggtgcaga acaaaggcag aaccttaacg catgaaatca ttttgtcacg
    47221 tatatgggga tatgactttg agggtgatgg cagcacagtc cacactcata tcaaaaattt
    47281 gcgggccaag ctgccggaaa atatcatcaa aaccattcgc ggtgtaggat accgattgga
    47341 ggaatcataa taatggaaag aaaagggatt ttcattaagg ttttttccta tacgatcatt
    47401 gtcctgctac tggttgtcgg tgtaacagca acactgtttg cacagcaatt tgtgtcttat
    47461 ttcagagtga tggaattaca gcaaacagta aaatcctatc agccattggt ggaactgatt
    47521 cagaatagcg ataggcttga tattcaagag gtggcagggc tgtttcacta caataaccaa
    47581 tcctttgagt tttatattga agataaagag ggaagcgtac tctatgccac accaaatgcc
    47641 aatacatcaa atagttttag acccgacttt ctttatgtgg tacatagaga tgataatatt
    47701 tcgattgttg ctcaaaacaa ggcaggtgtg ggattgcttt atcaagggct gacaattcgg
    47761 ggaattgtta tgattgcgat aatggttgta ttcagccttt tatgcgcgta tatctttgcg
    47821 cggcaaatga caacgccgat caaagcctta gcggacagtg cgaataaaat ggcaaacctg
    47881 aaagatgtac cgccgccgct ggagcgaaag gatgagcttg gcgcactggc tcatgatatg
    47941 cattccatgt atgtcaggct gaaagaaacc atcgcaaggc tggaggatga aatcgcaagg
    48001 gaacatgagt tggaggaaac acagcgatat ttctttgcgg cagcttctca tgagctaaaa
    48061 acgcccatcg cggctacaag cgttctgttg gagggaatgc ttggaaatat cggtgactac
    48121 aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga tggataggca gggcaaaatc
    48181 atttccgaaa tactggagcc tgtcagcctg aatgatggga gaatcgtacc catagctgaa
    48241 ccgttggaca tagggcgcac ggttgccgag ttgctgcccg attttcaaac cttggcagag
    48301 gcaaacaacc agcggttcgt cacagatatt ccagccgggc aaattgtcct gtccgatccg
    48361 aggctgctcc aaaaggcact atccaatgtc atattgaatg cagttcagaa cacgccgcag
    48421 ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa aatgccgcct ttttgttttg
    48481 aacatgggcg ttcacattga tgatactgcg cttccaaggc tgttcacccc attctatcgc
    48541 attgatcagg cgcgaagcag aaaaagtggg cgaagcggtt taggacttgc catcgtacaa
    48601 aaaacgctgg atgccatgag cctccagtat gcgctggaaa acacctcgga tggcgttttg
    48661 ttctggctgg atttaccgct cacatcaaca ttgtaaatat ttaaaactta aatgattttg
    48721 accgacaggt ataaccctgc cggtcttttt ttgctgctac aggaaaa
//
LOCUS       DAGCXG010000012        47470 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGCXG010000012 DAGCXG010000000
VERSION     DAGCXG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.012"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(55..789)
                     /locus_tag="MB022_000681"
     CDS             complement(55..789)
                     /locus_tag="MB022_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4465702.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="MB022_000682"
     CDS             1116..1526
                     /locus_tag="MB022_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBT4465703.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="MB022_000683"
     CDS             1519..2220
                     /locus_tag="MB022_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBT4465704.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="MB022_000684"
     CDS             2220..3806
                     /locus_tag="MB022_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465705.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="MB022_000685"
     CDS             3825..4046
                     /locus_tag="MB022_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBT4465706.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="MB022_000686"
     CDS             4043..4804
                     /locus_tag="MB022_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBT4465707.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="MB022_000687"
     CDS             5184..5693
                     /locus_tag="MB022_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4465708.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="MB022_000688"
     CDS             complement(5768..6307)
                     /locus_tag="MB022_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBT4465709.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="MB022_000689"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="MB022_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBT4465710.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="MB022_000690"
     CDS             7320..8171
                     /locus_tag="MB022_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBT4465711.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="MB022_000691"
     CDS             8199..8834
                     /locus_tag="MB022_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBT4465712.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="MB022_000692"
     CDS             8854..9627
                     /locus_tag="MB022_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4465713.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="MB022_000693"
     CDS             10035..10832
                     /locus_tag="MB022_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBT4465714.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="MB022_000694"
     CDS             10810..11223
                     /locus_tag="MB022_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4465715.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="MB022_000695"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="MB022_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4465716.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="MB022_000696"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="MB022_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBT4465717.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="MB022_000697"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="MB022_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBT4465718.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="MB022_000698"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="MB022_000698"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBT4465719.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="MB022_000699"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="MB022_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBT4465720.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="MB022_000700"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="MB022_000700"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBT4465721.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="MB022_000701"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="MB022_000701"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBT4465722.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="MB022_000702"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="MB022_000702"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBT4465723.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="MB022_000703"
     CDS             21907..22485
                     /locus_tag="MB022_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4465724.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="MB022_000704"
     CDS             22537..23019
                     /locus_tag="MB022_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBT4465725.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="MB022_000705"
     CDS             23136..23510
                     /locus_tag="MB022_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465726.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="MB022_000706"
     CDS             complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="MB022_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4465727.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25687..26277
                     /locus_tag="MB022_000707"
     CDS             25687..26277
                     /locus_tag="MB022_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4465728.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26405..27754)
                     /locus_tag="MB022_000708"
     CDS             complement(26405..27754)
                     /locus_tag="MB022_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4465729.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27922..28662)
                     /locus_tag="MB022_000709"
     CDS             complement(27922..28662)
                     /locus_tag="MB022_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4465730.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28675..30267)
                     /locus_tag="MB022_000710"
     CDS             complement(28675..30267)
                     /locus_tag="MB022_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4465731.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30835..31131)
                     /locus_tag="MB022_000711"
     CDS             complement(30835..31131)
                     /locus_tag="MB022_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465732.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31307..32032
                     /locus_tag="MB022_000712"
     CDS             31307..32032
                     /locus_tag="MB022_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBT4465733.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32025..32867
                     /locus_tag="MB022_000713"
     CDS             32025..32867
                     /locus_tag="MB022_000713"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBT4465734.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32870..33391
                     /locus_tag="MB022_000714"
     CDS             32870..33391
                     /locus_tag="MB022_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBT4465735.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33644..34207)
                     /locus_tag="MB022_000715"
     CDS             complement(33644..34207)
                     /locus_tag="MB022_000715"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4465736.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34458..34727
                     /gene="rpsO"
                     /locus_tag="MB022_000716"
     CDS             34458..34727
                     /gene="rpsO"
                     /locus_tag="MB022_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBT4465737.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34915..37041
                     /gene="pnp"
                     /locus_tag="MB022_000717"
     CDS             34915..37041
                     /gene="pnp"
                     /locus_tag="MB022_000717"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBT4465738.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37551..37817
                     /locus_tag="MB022_000718"
     CDS             37551..37817
                     /locus_tag="MB022_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4465739.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37994..39952
                     /locus_tag="MB022_000719"
     CDS             37994..39952
                     /locus_tag="MB022_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBT4465740.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40306..41607
                     /locus_tag="MB022_000720"
     CDS             40306..41607
                     /locus_tag="MB022_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4465741.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41737..43107)
                     /locus_tag="MB022_000721"
     CDS             complement(41737..43107)
                     /locus_tag="MB022_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBT4465742.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43289..43612)
                     /locus_tag="MB022_000722"
     CDS             complement(43289..43612)
                     /locus_tag="MB022_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBT4465743.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43937..44545)
                     /locus_tag="MB022_000723"
     CDS             complement(43937..44545)
                     /locus_tag="MB022_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBT4465744.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44875..45591
                     /locus_tag="MB022_000724"
     CDS             44875..45591
                     /locus_tag="MB022_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBT4465745.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45604..46299
                     /locus_tag="MB022_000725"
     CDS             45604..46299
                     /locus_tag="MB022_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBT4465746.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46383..47012
                     /gene="udk"
                     /locus_tag="MB022_000726"
     CDS             46383..47012
                     /gene="udk"
                     /locus_tag="MB022_000726"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBT4465747.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15391 a         8789 c         9878 g        13412 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat
    23881 aaaagaaatg ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt
    23941 atatggagct actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa
    24001 taaacggtgt tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga
    24061 agccattttc aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc
    24121 aataaacagt gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc
    24181 tcttttatca atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa
    24241 aaccgataac tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt
    24301 ccgcaacata taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt
    24361 tcaacccatc aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg
    24421 tacgctgctt aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat
    24481 agatgaacat caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg
    24541 ctaaacaagg aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca
    24601 aatctgtgaa tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta
    24661 atataacgat aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct
    24721 gagccagcgt gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta
    24781 aataagcgag caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga
    24841 tcgtgtcttt agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa
    24901 tgatgatccc aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc
    24961 ctgtgaaaaa agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta
    25021 gtggattcaa aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca
    25081 ggaactttcc aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca
    25141 caaaaaatac aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg
    25201 gtgctaaacc aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga
    25261 taactaagta aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta
    25321 attcatatac acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac
    25381 cgataccggt aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac
    25441 ccaaatgaac gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc
    25501 ctataaaaag atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt
    25561 ttttaaaagt aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa
    25621 aacgacggtt atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga
    25681 atcatcgtga caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg
    25741 agagttcttg tattagagat caagatccct aatacatcta gctttcattc tttatgggtc
    25801 atccgaaata cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg
    25861 aaccatcatc gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat
    25921 atctttttgc tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac
    25981 cctgaagctt tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct
    26041 tttggtatct tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga
    26101 gttttgtcaa aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc
    26161 ggtgtctttt ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg
    26221 attttttcct ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa
    26281 agaggctggg acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa
    26341 gaagcaattt tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac
    26401 tttcttactt cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca
    26461 caggtgttag taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa
    26521 aggccatgta aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat
    26581 gagctttttt gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc
    26641 agataaacaa gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca
    26701 tcacgtaatt caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc
    26761 ctggcacatg atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta
    26821 aagaacgaag catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac
    26881 ttaatacgac taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt
    26941 ctacaaatgg actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa
    27001 aaagagatcc taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca
    27061 acactttttc aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa
    27121 aagcaaaggt taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc
    27181 cgccatgaga ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga
    27241 ggatcatata agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa
    27301 accaaaactc taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac
    27361 ctaatgcaat cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca
    27421 ttccgatggc tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc
    27481 cagtgatgaa agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt
    27541 tattggtgtt tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc
    27601 cagtaataag ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac
    27661 ctaaaaacaa ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt
    27721 tcagtccacg ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt
    27781 ttaattactt ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc
    27841 agaaatttac cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt
    27901 tgtcccaatc agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg
    27961 ttcttctttc ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt
    28021 atccataaaa atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat
    28081 aagcattgtc aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat
    28141 ttcagggtct aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa
    28201 tgcccgagca attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc
    28261 gatataacga tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc
    28321 tttatctcgt ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt
    28381 gttgaacagg ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt
    28441 atatttagga gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc
    28501 cagtaaattg atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt
    28561 cacaacttca cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa
    28621 gctttttctt agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc
    28681 ctgcaaattt gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt
    28741 cttttctacc gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc
    28801 gacgatcgta aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa
    28861 gaacaaatca gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa
    28921 ctcatttcct gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat
    28981 cgtttggcca tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc
    29041 aaaaatcccc ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa
    29101 caaagcggca atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat
    29161 gaccattgct tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag
    29221 tgcatttccg attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg
    29281 gaagacacca atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa
    29341 caaggtaagt ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa
    29401 atcagaaatg atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc
    29461 ttggtccata attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt
    29521 ttggatatcc gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt
    29581 ctggaagcca tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc
    29641 cgttacacct tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt
    29701 tctcattgct gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat
    29761 cacagtatag tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa
    29821 actttttgct tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta
    29881 ataaggatcg gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat
    29941 gatcgcgtcg atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac
    30001 gatgactaat tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc
    30061 gccggcgtaa ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga
    30121 ccagttgaag ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt
    30181 ttcatctgca tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac
    30241 ggcaactgaa accatatttt ttttcatttt tttctccctt tctgattata tgatacacga
    30301 gcggtctaga tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata
    30361 gatataaaaa ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa
    30421 cttagcatca ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca
    30481 agcaaaaaag tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac
    30541 tccacagggt taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag
    30601 ttatttatac aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt
    30661 ttttgaaaaa atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat
    30721 gtgaatcatc tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca
    30781 ataaaaaaag aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc
    30841 tgccgctttc tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat
    30901 ggctaatatt agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata
    30961 tacggctaat gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca
    31021 aagatttcca ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa
    31081 ccaggcgata caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca
    31141 cctcactttt atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg
    31201 ataaattgtt caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat
    31261 aaatttgttc gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac
    31321 agtagacgaa gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc
    31381 aggggtatcc acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg
    31441 gatggagcag ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt
    31501 ctatcaattt gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga
    31561 aatggcacat ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt
    31621 gcacgcaaag gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca
    31681 ttgtcgaaaa tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca
    31741 agcatgcgga ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg
    31801 agtcatggag caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac
    31861 gagttttcaa gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat
    31921 cttagtaatc aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga
    31981 cggagcggtt gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa
    32041 ccgaaaaaga aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg
    32101 ccgaccgtat gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac
    32161 tgcgatccca attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc
    32221 cagttatcaa ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt
    32281 tcaattgtac atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc
    32341 cgaatgtcca gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc
    32401 tggagtacgc aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg
    32461 tcacgccggg tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca
    32521 aatcttttcc cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag
    32581 aggaagaact aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc
    32641 gagagttgat tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga
    32701 aaaaagaaga aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag
    32761 ttgttagtct gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt
    32821 tgatgaaaca ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg
    32881 agagacacgg cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc
    32941 tagcaagttt gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt
    33001 actacgagct gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga
    33061 agaaaccaat actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga
    33121 agaagaactg agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca
    33181 tcccctttac ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc
    33241 ctttatgaga cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat
    33301 ccatctgcat acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact
    33361 agctgaagca ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa
    33421 actattctat catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa
    33481 gatgcgtatc caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt
    33541 tcgattagtg gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat
    33601 aaagctttct tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata
    33661 cgccttcttt tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt
    33721 tgatatggtc gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt
    33781 gtttttcacc attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc
    33841 ctggtacttc tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa
    33901 cagaatggct caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct
    33961 ctgggtcagg acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag
    34021 cagctaagcc aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt
    34081 tttttaagaa ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag
    34141 aaacttcttt tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg
    34201 taatcatgga atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta
    34261 tagtttacca caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa
    34321 tgattaacta cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca
    34381 catgacctgc cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat
    34441 cgaaagaggt gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat
    34501 acgctcgcca tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag
    34561 aaatcaacca cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg
    34621 gcctaatgaa aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg
    34681 ttcaacgtta ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa
    34741 gagtgggatt cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt
    34801 gatcaagcta gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc
    34861 agttttcatt tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca
    34921 gaaaaacaag tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag
    34981 ctagcaaaac aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt
    35041 gcagctgtgg cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat
    35101 gaagaaaaaa tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt
    35161 ccaagtacgg aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt
    35221 gcagaaggct tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac
    35281 tgctcgccag ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc
    35341 ccatttgacg gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg
    35401 aatccaacag tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa
    35461 caagccatca atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt
    35521 gcattgctgt ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt
    35581 caagcagtag ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa
    35641 aaagaaatct ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa
    35701 cttgctcgcg aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag
    35761 aaatttgcag gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa
    35821 gatcttgaaa aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg
    35881 cgtaaattgg atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat
    35941 ggatcaggtt tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct
    36001 ctaggagagc accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac
    36061 cactacaatt tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt
    36121 cgggaaatcg gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa
    36181 gaagaatttc cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct
    36241 tcccaagcaa gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa
    36301 gcgccagtcg ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta
    36361 actgatatcc aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca
    36421 aaagatggta tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc
    36481 ctaactgaag cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact
    36541 tcaacgattg ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc
    36601 cagatcgaac ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga
    36661 atcatcgatg aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct
    36721 tcagctgatg cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa
    36781 gtcaaagttg gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc
    36841 gtcaacttga tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt
    36901 gtcaataaag tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa
    36961 atcgataaac aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca
    37021 acgaaaaatg aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt
    37081 gacaggggga caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc
    37141 ttttttttcg aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt
    37201 taaaaacatt aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt
    37261 tttacaacct ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa
    37321 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcaggt caaaccgctg
    37381 atttcacaac ctctgattca cggtgttcca gaagcagctt ctacggaaat aagctgaaca
    37441 cttttgttac aacttctttt tttctggagt ttcttggcgc atatttttgg agtccttctg
    37501 aataaagcgt aaaataaaag tgagaaatat ggataaagga gggaaaggag atgctgaatt
    37561 ttttatggtc gttatttatc ggcggtatat taggtttgat agctggcgca attttaggaa
    37621 aggaagtccc gggaggcgta tctggaaata tcattatcgg ttttttaggt agttggctcg
    37681 gcgaattttt actaggacca ctgggaccgg aactcggcgg tttttatttg ataccagcat
    37741 tattgggtgc aattttttgc cttgctcttt attcatttgt ggcaagacgt atcagagaac
    37801 gtccaaagaa agaataattt tattcaaata agttgacact tcagtgtgtt acttggataa
    37861 ttagaagcaa tttaggaaag cgggtgtcat ggataagaat atcatctatc attcaagctt
    37921 acctaaagga tatccatata acaagaaatg agggaaaaac gtggcaacaa cgcacaatac
    37981 caaaaagata tcaatgactg gcctgcttgt agcgatggga gtcgtctacg gagacatcgg
    38041 gacaagtccg ctttacgtta tgaaatcgat tgttgaagat aatggcggat tgtcaggaat
    38101 caatcctgat tttgttatcg gatcagtctc attgattttt tggacattga ctctactgac
    38161 aacaatcaaa tatgtcgtga ttgcattgaa tgcagataac catggtgaag gtggaatttt
    38221 ctcactctac acacttgtcc gaaaaggtgg gaaatatttg atcattcctg caatgatcgg
    38281 aggagctgcc ttactagcag atggggtatt gacccctgct gtgacggtaa cgactgcgat
    38341 tgaaggcttg cgaggaattc cgcagttttt tgaccggttc ggtaataacc agaacgtgat
    38401 cgtcattatc actttagtta ttatttttgt tttattcatg gtccaacgct ttggtacaga
    38461 tattgtcgga aaagcattcg gaccaatcat gttcgcttgg tttacttttt taggcgtaat
    38521 gggattaatg aacttcagtc aagactggac agttatccgt gcactgaatc cttattatgc
    38581 tatccagctg ctttttagtg cagataacaa attaggcttg ttcattcttg gaaatatttt
    38641 cctggctacg acaggtgcag aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa
    38701 tattcgtctg agctggcctt acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc
    38761 tgcgtggttg ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga acctgaatcc
    38821 attcttccaa atgatgcctc aaagttggat cgtgatcggc gttgcttttg caacagtcgc
    38881 tgcagtcatt gcttcgcaag cattgattac aggttcgttt acgctagttt cagaagcaat
    38941 caagttgaaa ctgctgcctc ggttaaagat catgtatcct gggaacagta tcgggcaaat
    39001 gtatatccca gcagtcaatc tgattttatg gatcgcctgt tctctagtgg ttgtcacatt
    39061 ccgaacgtct catcatatgg aagctgctta cggtctatcg atcactgtga cgatgttgat
    39121 gacaacagca ctgctttact tttatcttat ccaaaatggg cattctcgtt ggcttgcata
    39181 tttagttacc tttttcttcg gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa
    39241 attcttccga ggcggttttg tagccgttct gattggtttg atcatcttag tcgtgatgtt
    39301 tatttgggaa caagggaaca ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga
    39361 ttatatcccg cagcttagag aattacgtga tgatacttct ttaccaatct atcagacaaa
    39421 tgtggtcttc ttggttcctg atatggctga agataaagtt ggccgacagt tcatctattc
    39481 gattttagat aaacggccta aaagagcacg agtctattgg tttgttcatg tggaagtgac
    39541 ggatgagcca tatacgaaag aatatcaagt agatatgatg gacactgatt ttattgtcca
    39601 agtgaatctg ttcttaggtt tcagggtcca acaggaaatc aacgtatatg ttcgccaagt
    39661 catccatgat ttgatgaaac aaggccgttt gccaaaacag ccacaaagat actctctgac
    39721 accagggcga gaagttggcg atttccaatt tgtcctgatt gatgaagtag tttccaatgt
    39781 gacaactcta ggaaaatggc aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat
    39841 cgctacctcg ccagaaacat ggtttgggct agagtacagc gaagtaaaac atgaaactgt
    39901 tccgcttatc attggaaata ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg
    39961 ctttgacctg aactaatgtc gaatgtcagc tacttgaaca ccgtttatca gaggtcatga
    40021 aaaagctgta ctgcaacaag cagtaagaac gagcagataa aaacagcttc tcatgttttt
    40081 catctgtttg ggcttaatgc cgtagatgca agcttttgaa taccgttttt atcagaggtc
    40141 atgaaaaagc tgtttagcaa caagtaacgc aatgattttg aagtgataag gagatgtggc
    40201 tgaaaaatgc cgcatctcct tttttattcc taaatatgaa tacgcattat tttcaatctt
    40261 tttgtagaaa aagggtacaa tagaaaaaga gaaaggcggg agaatatggt tatcaatttc
    40321 attattttat ttttgatgat tgcaattact tcattttttg tagcaagtga atttgcttta
    40381 gtgaaaatca gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag
    40441 ctagctcttc atgttacaca ccatttagat aactatttat cagctagtca attaggaatt
    40501 acgctaaccg gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa
    40561 ccagtgatcg gtaaactacc ttttagttcg gctattagca gtacaatctc tgtggcgctg
    40621 ggatttattt tagttacgta tatcgatgtg gttattggcg aactgcttcc taagagctat
    40681 agtatcgtca atacggagaa ggtcgtcttg ttcgtagtta aaccacttca ttatttttat
    40741 aaagtaatgt ttccttttat ttgggtattg aatcattcag ctgcagggct agggaagcta
    40801 ttaggtgtgc gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga gctgacatta
    40861 gtcgcattga attcttatga aaaaggagag ttgaccaaag aagaatatta ttatttggaa
    40921 aacgtgtttg aatttgatga taccttggca aaagatatac aagtagaccg tacgtccatg
    40981 caagtattcg aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga
    41041 catactcgct atccggttat cttagaatca aaagacaatg ttttaggtta tgtcaccttg
    41101 ccagatttga tcaagcaatc ttataaagat gaccagctga cagtagaaca gttgattgaa
    41161 gagccgatcg taacaactga aacgatccct ataaaaaaat tattgacgat catgcgaaaa
    41221 aagggtaagc atattgctat cttaaaagat gaatacggag ggacaagcgg cttagtgacg
    41281 attgaagata ttttagaaga aatcgtcggg gaaatccgag atgagacaga tttggacgaa
    41341 gcattgatag cggaacagtc agatggctct tatatcatct ccggcaaact aacattagat
    41401 gatttccagc gttattttca tgtagagatt ccagagtttg aagaaacgaa ttttactaca
    41461 cttgcgggct ttgcttctag ccgatataaa gaaataaaag caggaacaat cattgagatt
    41521 gcatcattcc gtttcacggt attagaatac cagcatgcac atatcgatta tttcaaagta
    41581 gagtccacgg aaagaaaaac agaataaaag aaaaaaggct gtgacatttt ttgtcacagc
    41641 ctttttgtta tctaaaaaac ctgttttcgg taaacgaaga agatattttc aataaaagtt
    41701 ttcattttta gccgcaacga tttcttttaa tcaaggctat cttgcttctg catgttctaa
    41761 tgtgaaatgt tcctcttcac ccatgtgttt caattgtttc atcgaacgca tataagagta
    41821 aagcaacacg gcaacagaac cgatccaagc taaaggagaa gctgctgctg ctcctgggta
    41881 tccccacatt cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt ccatcatacc
    41941 ggcgatcgta gggatcttgc tttgtccaag cccttgaagt gtgtagcgta aaatgaacag
    42001 gactgctaag atccagtaaa gagacccatt gatattgaag taggtttgtg ctaattcaaa
    42061 tacacgtgtt tctgatgggc taacaaacaa ttgaacaaag aaatgaccga agaaaatgac
    42121 gatagcacct gctaagagac taaatccgac actcatcagt aaagtttgac gtaccccttg
    42181 caagattcgg ccatattctt tcgcaccata gttttgtgca gtgaaagttg ccattgcaat
    42241 accaaatgac atcatcggct gatttgcaaa ttggtcgata cgaccagcag cggcttgagc
    42301 agctacgaca tctgtcccta gactatttag tgctgcttgt aacacgatag caccaacagc
    42361 gatgatcgaa gattgaaacg ccattggcaa agcagcattt aggtgtacag caaactcttc
    42421 tttttcaaat ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata
    42481 cactagacac agtacgctgg aagaaacttg tgcgattact gtagcaatag ctgcaccttc
    42541 cacacccatg ccaaaataga taataaagat caaatcaagt acgacgttga tgatgaccgc
    42601 aatgatcaga aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag
    42661 caaattgaaa ctgactgatg caaagatccc taataaaata attgaaataa atgtttgtgc
    42721 ttgatcaagt atttcaggag gagtttgcat caattgaagc atcggacgaa taaacaggag
    42781 acttaggaca gttaaaatca cagtgacgac aagactaatg acaacactag cagcaaaact
    42841 tttcttcaga ccccgataat cttttgcacc gtaacgttgt gcggtaataa tggctaaacc
    42901 agctgttaaa ccttgggcaa agccaatgat caaaaatgtc aggcttccag ttgcaccaac
    42961 cgctgccagc gcatttttac ctagcgtctg acccacgatg atcatatcga tcatattgta
    43021 gaactgctga aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc
    43081 ggggcttcct tttgttaaat cgcgcatctt ttaaactcct tatatcttta aaatttagtt
    43141 agtagtatat agcgtttatc ctgagaatac caattagaaa tcaatgataa tttatgaaac
    43201 tccataaaaa gaagccaaga attgagtcaa agaatcaaca acttggcctg gtctgtaaaa
    43261 taagggacat ccacgtttat ctatcacctt aatattgtct aatctcactt ggtaaaagga
    43321 tcgtttctct cgtaccgtca tcataggcgt atacttgagc aggaaattgg ttcgaatagg
    43381 caaacggcaa atcaatatcc atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca
    43441 cttttccacg attttcgatc aaatcaaagg ttaatatatt ttctaatgga atgactccct
    43501 taagatctag gtcaataata aaccatatac tgtcaatcaa ttcatctggc aaacttgata
    43561 cgacaccaac tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac atctgtttaa
    43621 cctccttcga atcaacgaat gttttatttt tataattaat cctaggataa actagaaata
    43681 cttacttcaa cgagggttca ttctaataaa gaatcaggaa aaatgcaaca ataattgaat
    43741 actttttttc aaagtataaa tttttataaa ggtacgataa aagtatgcga ggaaaagctt
    43801 ggtaaattag gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa
    43861 taaacgatta gaaataaaaa aggcgtgtta aaagctgctg acccgccgct tctaacacca
    43921 cctgattgtt tcagtattat tcttctgttg aaccgatcac ttctggttcg ccaccttcac
    43981 ctgtgctttc ttcttcttca ctttgagcga ctacagcagc gatttgctct tcactgtcgg
    44041 tcacgaattt gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa tcacctaatt
    44101 ccaagtttgt cacgtcaact tcaattcgtt caggcaattt gtctggtgta gcagatacga
    44161 ctactgtata caagttttga gctaacactc caccagattt agcacccact gattcgccaa
    44221 caagaacgac ttctgtttcg acttctgttt cttccgacat gtttacagat agaaattctg
    44281 catgttcgaa atgattggta aaagtgttcg tttggatctt gtgaaccaat gtattgactt
    44341 tttgtccatc gatgtccatc gtgatgactg tgttcgctcc atgttcacgc aacaatttgc
    44401 ttagtgtcaa accatcgaat gagatagggg tactttctac tttataccca taaacaacag
    44461 ctggaacttt cccttcgtga cgaagtttgt ttcttaatga acggggacgt acttctcttt
    44521 tacttacttc taatgatact gacataacca aaattcctcc ttaaaatgtg actgtctgct
    44581 ttggtaacgg acagatttta tttaagtgaa ttctaaaggc taatctcgtg cacatcatcg
    44641 aaggcagggc gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc ttagaaagtc
    44701 aggattcaac ttttctccac caggtcatta cgctgtgtat gagaaatcca attcacttta
    44761 ctatttaacc gtaacgtgaa ctgttaatta agtcaaggaa taactatgat gatgtatttg
    44821 caagcaagaa tattcaaggt ataattaatt agtagtaata agaaaggaaa cagcatgcgt
    44881 ttagataaat taatagaaga aaaattagaa acgacacgca aggaaatgaa gcggctgttt
    44941 gctttgaagc aagtgatgat agatggcaca gtcgaatttc gacaaaatcg aaatgtagat
    45001 agtttactgc atcaaatcga agtagcaggt gaacacttag aaacaaatga gtcctattat
    45061 cttctcaata aacctgaaaa agctgttaca gctgtatcag atccagaaaa gacgacagta
    45121 atcgatctga ttgcaagtgc cgatcgtttc caaccattat atccagtggg aagactagat
    45181 agagatacaa caggactatt gctgatcact tcgaatggac aattgggata tgaaatgcta
    45241 cttccagaaa aaaaggtttc aaagacttat caagtagttg tgaacgaaag ggtgacaccg
    45301 gcagacgtaa cagcatttga aaatggaatc gtattcgctg atgggtatca atgtaagccg
    45361 gctgatttaa aaatactcaa agcagacaag caccaaagta cggttttatt gacgatccaa
    45421 gaaggaaaat tccatcaagt caaaaagatg ttcttagccc aaggaaagaa agtgaccgca
    45481 ttgaaacggc taagcatggg accattggaa ttagatgaac agctaggagt aggcgagtat
    45541 cgatcgttga cattagcaga gatgaaaaag ttaagtattt attttaaata ggaggtcaaa
    45601 aagatgaaat ataaaacttt attgtttgat gtagatgata cattgttaga ttttcaatta
    45661 actgaagcca atgctttaag tgctttgttt gcagaacaag gaatcacatt gacaccagaa
    45721 atcgaaacca gctataaggc tttgaatcat catttatggc aggagtttga aaaaggcaga
    45781 ctatcacgag aagaaataat aggcagtcgt tttgggttac ttttccagca atttgggaaa
    45841 acagtagata gtgatcaaat ggaaaaacgc taccgccact acttgaacca aggtcatgat
    45901 cttgttccag gaagtctaga aatcctgaaa aaggtccatc cgcatgctga tctttatatt
    45961 gtgacgaacg gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg aatgcttgat
    46021 tacttcaagg atatctttgt ctcagaagct gtcggagcgc aaaagccgat gaaagaattt
    46081 tttgatcatg tcttttccca cattcctaat tttgataaaa agcaaactgt gatcattggt
    46141 gattctttga cttctgatat caaaggagga aatatggcag ggatcgatac gatttggttc
    46201 aatagaaata aattaccaga aatcgaagaa atccagccta cttatcgaat cgattcatta
    46261 gaggaacttt attcattatt ggaaattgaa aaggattaat tttcagaaaa atatagcatt
    46321 ttcgtgtttt ttcgctataa tagattagaa tgaaaaaacg gatacgaaag aggttttagt
    46381 caatgacaaa aagcaagcca attatcattg gtgtgacggg tggatccgga agcggaaaga
    46441 caagtgtcag ccgtgcgatt ttcaacaact tcccggacca ttccatcatg atgcttgaac
    46501 aagattctta ttacaaggat cagagccatt tgagttttga agaacgattg aacacaaatt
    46561 acgatcaccc atttgccttt gataatgatc tgctgattca gcatgtaggc gacttactga
    46621 actataaagc cattgaaaaa cccgtgtatg actatgtagc gcatacaaga agtcaggcaa
    46681 cgatcattca ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt ttagaagacg
    46741 aacgtctgcg tgacttgatg gatatcaaag tatacgtaga tacagatgat gacattcgta
    46801 tcattcgtcg tatcaaacgg gatatggaag aacggggacg cacattggac tccgtgatcg
    46861 aacaatattt gacagttgtt aaaccgatgt atcatcaatt tatcgaacca acgaagcgct
    46921 atgcggatat cattgttcca gaaggcggag aaaaccacgt tgctattgat ttgattacaa
    46981 caaaagtagc tagtttctta aaccataaat agtgcgcagc gaaacagttg aacggactgc
    47041 gaccgtttga aaagcaagga ttctgctttt tgaacagtca ccagccaact gtttttttat
    47101 ttataacaag aggttgtgaa aacagcatct tgacctaagt attaaaaaga agaaacggaa
    47161 aatagctgtt tatattttac cgttttttga gctaataccg aaggtcagct gattgagcac
    47221 cgtgaagtag aggttgtaaa aaagctgtct ttgcatcaag cgataagagc atccaaataa
    47281 aacagctcct cacgtttttc gttgggttgg gcttattatc gtagatgcaa gcttttgaac
    47341 accgtttaac gagaggttgt aacagaagtg gtcagatgca ataaataaga agaaacttac
    47401 gaaaattatt tctcgaattt gtgtgagatt tcgacttatt tttttgaaga agctcttttg
    47461 tcgcaccccc
//
LOCUS       DAGCXG010000013        46521 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGCXG010000013 DAGCXG010000000
VERSION     DAGCXG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.013"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            33..476
                     /locus_tag="MB022_000727"
     CDS             33..476
                     /locus_tag="MB022_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4465748.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(959..1489)
                     /locus_tag="MB022_000728"
     CDS             complement(959..1489)
                     /locus_tag="MB022_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBT4465749.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1594..2145
                     /locus_tag="MB022_000729"
     CDS             1594..2145
                     /locus_tag="MB022_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4465750.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(2298..2771)
                     /locus_tag="MB022_000730"
     CDS             complement(2298..2771)
                     /locus_tag="MB022_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4465751.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2909..4381
                     /locus_tag="MB022_000731"
     CDS             2909..4381
                     /locus_tag="MB022_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4465752.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            4378..5088
                     /locus_tag="MB022_000732"
     CDS             4378..5088
                     /locus_tag="MB022_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBT4465753.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            5440..5724
                     /locus_tag="MB022_000733"
     CDS             5440..5724
                     /locus_tag="MB022_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBT4465754.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(6005..6424)
                     /locus_tag="MB022_000734"
     CDS             complement(6005..6424)
                     /locus_tag="MB022_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBT4465755.1"
                     /translation="MEKETIVEVTKLGFTIQTAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(6426..7010)
                     /locus_tag="MB022_000735"
     CDS             complement(6426..7010)
                     /locus_tag="MB022_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBT4465756.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(7057..8532)
                     /locus_tag="MB022_000736"
     CDS             complement(7057..8532)
                     /locus_tag="MB022_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBT4465757.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8713..9024
                     /locus_tag="MB022_000737"
     CDS             8713..9024
                     /locus_tag="MB022_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465758.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNILALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(9139..11475)
                     /locus_tag="MB022_000738"
     CDS             complement(9139..11475)
                     /locus_tag="MB022_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4465759.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            11617..12285
                     /locus_tag="MB022_000739"
     CDS             11617..12285
                     /locus_tag="MB022_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4465760.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            12288..13331
                     /locus_tag="MB022_000740"
     CDS             12288..13331
                     /locus_tag="MB022_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4465761.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13568..14449)
                     /locus_tag="MB022_000741"
     CDS             complement(13568..14449)
                     /locus_tag="MB022_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4465762.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            14608..15336
                     /locus_tag="MB022_000742"
     CDS             14608..15336
                     /locus_tag="MB022_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBT4465763.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            15492..15809
                     /locus_tag="MB022_000743"
     CDS             15492..15809
                     /locus_tag="MB022_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4465764.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            15802..16410
                     /locus_tag="MB022_000744"
     CDS             15802..16410
                     /locus_tag="MB022_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBT4465765.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            16465..17256
                     /locus_tag="MB022_000745"
     CDS             16465..17256
                     /locus_tag="MB022_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBT4465766.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            17394..17882
                     /locus_tag="MB022_000746"
     CDS             17394..17882
                     /locus_tag="MB022_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4465767.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            17933..18805
                     /locus_tag="MB022_000747"
     CDS             17933..18805
                     /locus_tag="MB022_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4465768.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            18981..19343
                     /locus_tag="MB022_000748"
     CDS             18981..19343
                     /locus_tag="MB022_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4465769.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            19352..20104
                     /locus_tag="MB022_000749"
     CDS             19352..20104
                     /locus_tag="MB022_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465770.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            20118..21920
                     /locus_tag="MB022_000750"
     CDS             20118..21920
                     /locus_tag="MB022_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465771.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            22197..23237
                     /locus_tag="MB022_000751"
     CDS             22197..23237
                     /locus_tag="MB022_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4465772.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(23289..25406)
                     /locus_tag="MB022_000752"
     CDS             complement(23289..25406)
                     /locus_tag="MB022_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4465773.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(25763..27136)
                     /locus_tag="MB022_000753"
     CDS             complement(25763..27136)
                     /locus_tag="MB022_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4465774.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(27136..27531)
                     /gene="rbsD"
                     /locus_tag="MB022_000754"
     CDS             complement(27136..27531)
                     /gene="rbsD"
                     /locus_tag="MB022_000754"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBT4465775.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(27559..28476)
                     /gene="rbsK"
                     /locus_tag="MB022_000755"
     CDS             complement(27559..28476)
                     /gene="rbsK"
                     /locus_tag="MB022_000755"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBT4465776.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(28584..29597)
                     /locus_tag="MB022_000756"
     CDS             complement(28584..29597)
                     /locus_tag="MB022_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4465777.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(29678..30277)
                     /locus_tag="MB022_000757"
     CDS             complement(29678..30277)
                     /locus_tag="MB022_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4465778.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            30463..31164
                     /locus_tag="MB022_000758"
     CDS             30463..31164
                     /locus_tag="MB022_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465779.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            31174..33885
                     /locus_tag="MB022_000759"
     CDS             31174..33885
                     /locus_tag="MB022_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465780.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            34282..35910
                     /gene="treC"
                     /locus_tag="MB022_000760"
     CDS             34282..35910
                     /gene="treC"
                     /locus_tag="MB022_000760"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBT4465781.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(35970..37151)
                     /locus_tag="MB022_000761"
     CDS             complement(35970..37151)
                     /locus_tag="MB022_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4465782.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            37403..38926
                     /locus_tag="MB022_000762"
     CDS             37403..38926
                     /locus_tag="MB022_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4465783.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(38995..39723)
                     /locus_tag="MB022_000763"
     CDS             complement(38995..39723)
                     /locus_tag="MB022_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4465784.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(39736..41016)
                     /locus_tag="MB022_000764"
     CDS             complement(39736..41016)
                     /locus_tag="MB022_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4465785.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(41157..41759)
                     /locus_tag="MB022_000765"
     CDS             complement(41157..41759)
                     /locus_tag="MB022_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4465786.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            42004..42210
                     /locus_tag="MB022_000766"
     CDS             42004..42210
                     /locus_tag="MB022_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465787.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            42522..43241
                     /locus_tag="MB022_000767"
     CDS             42522..43241
                     /locus_tag="MB022_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4465788.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(43322..44005)
                     /locus_tag="MB022_000768"
     CDS             complement(43322..44005)
                     /locus_tag="MB022_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBT4465789.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            44223..46409
                     /locus_tag="MB022_000769"
     CDS             44223..46409
                     /locus_tag="MB022_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4465790.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        15082 a         8262 c         9285 g        13892 t
ORIGIN      
        1 ttaaaatcaa tgaataagta aggagagaaa aaatgatccg cacaacaaca aaattgtctg
       61 ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc taacttggat tctcatgaat
      121 tcattccatc aacttattgg gagaatcaag tagaatctgt taggaaagct ttaccagatg
      181 cagtgattta cttagcagaa atagatcaag aaattattgg ttttgctggg gtgattgatc
      241 agtttatcgc aggaatcttt gtgaaaaggg aataccggca acaaagaatc ggtacgcaac
      301 taatgaaaaa aataaaagaa accaataaaa cactgacttt gacagtctat caaaaaaatc
      361 aatcagctat tcatttctat ctttcacaag gttttacaat tcaaacacag caaaaagatg
      421 aagcagtaaa tgaggaggaa cttgtgatga aatggaatca aaacgctgat ttttgatgaa
      481 cagaataacg aaataatgct cccagcctaa ttttttgctg ggagcattat ttcattttga
      541 agtcactaat ttaactgtgt gaatatgcag ttgttacaat ctttgataaa cggtgttcaa
      601 tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccatt
      661 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttgataa acggtgttca
      721 aacggctgct tctcggtaat aagcccacta gcctagaaaa tatgaaaagc tattttctaa
      781 ctagcgttct tattactcaa agcataacgc tgatttcaca acctcttgat aaacggtgtt
      841 caatcagctg accctcggta ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc
      901 gtttctttct ctaatactca ggtcagaacg ctgatttcac aacctctctt tcacaacctt
      961 attttactcg tttataattt tcatcgaagt attttccgct acggatatca tctggcattc
     1021 catcgtatgg ttcttcgcta ataacatctt cattgtctat acctgcatca cccatataag
     1081 tgatcgtagc aaattcagga acaagtaatt ttggataagt ttcatatttt tcacgagctg
     1141 cttccatttc tgcgatatgt tcgttttgga aagtaacagt gatttctgga tgttcttcaa
     1201 cccatttagg gaaaaagatc attccgtgaa gtttcttttc atttggcata aagtccaatg
     1261 ccacgtctgt ccctgttggc tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa
     1321 cgatatcagc ttcttggtct tttacccaac ggcctttgac cattccgcca tggatacggt
     1381 aatcaacagt atggtcattt ttggcatacc attcatattc ccaaccattg tcatacgtat
     1441 agataaagtg agttccaata aaatcttcca attctttaaa ttctttcatg tttaagcatc
     1501 tccttataaa ttaaaacatg taataaacta catgttttcg attactcatt tatgatagaa
     1561 tggatttaaa gatttgtcaa ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat
     1621 attttgttag ggttattaag taagaagaag atgacaggat atgaattgaa tcaatcgttc
     1681 aaaaatgaaa taggagaatt ttggcaagca aaacatagtc agatttatcc tgaacttgca
     1741 aaaatggaag agcaaggaat cattcagcat caagtagaga tcacaggtga aaaattggaa
     1801 aagaaagttt atgaattaac agaagaaggg aaggaacaat tggcagagtg gattcataca
     1861 ccaaccaatg aactaccggt taatcgagac gaatttgttc tgaaattgta tttcgtcaaa
     1921 gacgtgaatg atccggcatt agtagaaatt attcagcagc agcgtgacct tcatgaagaa
     1981 aaacgacttc atctattaga gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat
     2041 gaaaattacg gacactacct gattttagcg catgcgatca atcgggaaac agaatatgca
     2101 aattggctaa cagatgtatt agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca
     2161 gtgatggaga attcctttct atatgaaaga aacgcagatc accgttcgga tattcttatg
     2221 ttcaaacagg ccttttttca agtatttatg tagcatctac ttattaaaaa gcttggtact
     2281 taggagatta gtggcgttta gagttctttt aacaactgtt cgtagtcagc tttttcatcg
     2341 acaggaagga gcttgtaagt taattcgtct gtctcgttgt catgaatgat gtacatctta
     2401 gcattctctt ctttgaagtt accgatttgt aaaatcgtat agcgttcgtc gatcaattcg
     2461 atatctttga ttgcttttga aaaaacttta cggacatggt gcattttctt ttggcggatt
     2521 tctgcctttt ctgcagcttt tgacaaagta aatccgtcat ctaaatattt tttgatcagt
     2581 tctactttta tgactgtagg taaacgatat tttctaggac aattcgcatc agcttcgatc
     2641 gaacgaatga atcccttttg ttcccaatac cttaattgac gtggagacac acctacgata
     2701 tcacttaatt cactgatgcc aacaaccaat tgatcatttt ccaacaaacg tttaagggaa
     2761 gccccaacca tttttgtcac tccttttgtc ctcttgcttc ccttaattat atccgtatta
     2821 acttatgtgt caagtaggtt gacaaatagc gaatttagga ctaaagtatt ctagtagttt
     2881 cagaaaaaat tcagaaagaa ggagaaatat gagtaacaac caacccgtag atatttacgg
     2941 aaaaccatat aatcggacac ttttagttgt tgtcttatta attggtacat tttgtaccgt
     3001 tttgaatcag accttattga cgacagcttt tcctacattg atgaaagctt tcgatatttc
     3061 agcgtctgat gtgcaatggt taacgactgg tttcttgcta gtcaacggga tcatgatccc
     3121 aataacagca tggctgatca ataaattcag ttcaagaaat ttatatcttt ctgccatgac
     3181 gatctttttg atcggaacga ttacttgctt tacagcacct aattttagta cattactaat
     3241 cggtcgcttg atccaagctt gcggtgtggg agtatcgatg ccattattgc aaaatatcat
     3301 gctatctatt ttcccgccag aaaaaagagg atcagcaatg gggatgtcag ggatcgttat
     3361 tggacttgct cctgctttag gccctacatt atctggttat atcattgata actaccattg
     3421 gcgtgacttg tttggtatgg tactgccaat cgttgttctt gtcttggttt tagcattctt
     3481 cctaatgaaa agtgttattc cattgtcaaa tcctagcatt gatattttat ccgctatttt
     3541 atcaacgatt ggattcggta gtttacttta cggattttct agtgtaggag atgacggctg
     3601 gggaagcttg aaagtaattg gtttcttagt tgttggtgtg attttcatcg ccttgttctc
     3661 atggcgtcaa ttacatttgg aacatccatt cttggaactt cgtgtattca aatcatctac
     3721 attcacgatt gcagctgttt tagctggtgt aacaaatatg gcgatggtag gggcagaaat
     3781 ggtcgtacca ttgtatattc aaaatattcg tggagaatca gcattccatt ctggcttgat
     3841 gttgttgccg ggtgcattga tcatgggggc tatgatgcct gtgacgggtg cgattttcga
     3901 taaatacggc gcgaaacgtt tagccatcac agggatgttc attttgacag cggcaactgc
     3961 accatttgct ttcttgacaa aaacgactcc agttttatat atcgtgatcc tttatgcgat
     4021 ccgtatgttt gggatctcca tggtcatgat gccggtaacg acttctggta tgaatgcatt
     4081 gccaaataac ttgatcagtc atgggacagc ggtaaataat acattccgtc aaatcgcaag
     4141 ctcgattggt acagctattt tgatcagtgt gttgacgaat gtgacaaaag caaatatccc
     4201 aagcaaatct gtattggata atacgccatt agcttataaa gatcaggcaa tcaatgccac
     4261 actgtcaggt taccatgccg cgtttatcgt agcgatcatc tttgggattc ttggctttat
     4321 tattgccttt ttattgaaga aaaaagaacc tgttctaatg aaagcggggg ataatgcatg
     4381 attattggag ttttgattgt cagcttgctt gcttttgctt taacgaacgt gttcgctaaa
     4441 aaaacatggc aaaccttttt atctttgatt ttcggactaa tcttcgtcgc ttcactaagt
     4501 ttgatcgtcg ctaatttgtc caatcacttt ggaatggaaa aagtaacgga aacaaaaaca
     4561 gaaaaaattg tttccagtgc tgattcccaa ggagcagata tgcttttgta taaagcttta
     4621 gggaatggaa aagaaaaagt gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa
     4681 gcaactggta cggataacga aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa
     4741 gtgacgaaaa ctacttattg ggaatacaaa aacgacatgt ataaattctg gttcaatatt
     4801 gctgataaca atcacgaata tgacagccga gtgaatacat tcaaaatccc agagacatgg
     4861 gtagaattaa gtacggacca agcagccaag ttagctgaat tagtgaagaa acagcaaagt
     4921 acgatggaaa gtgaagcaaa agcttatgtt caagacggta tggtaaaagc aatgactgaa
     4981 aatccaaaaa tgagcaaagc agagcaagaa caacgtacga aagccttagc tgctgaattc
     5041 caacaacaag cattcgcaaa attagtgaaa gaagcaaaag gcgaataata ttttgaacac
     5101 catgtaggag gttgtgaaaa agctgctctg catcaagcag taagaacaat cagataaaaa
     5161 tagcactcca tatttttcat ctgattgggc ttagtgccgt agatgcaagc ttttgaacac
     5221 cgtttaagga agaggttgtg agcctgccgt ttaactctga gtcacaagga gcaatttcaa
     5281 aaaacagctt ctcatgtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa
     5341 caccgtttat tcgaaaaaag gaactgcgac aagacttttg tcacagttcc ttcttttttt
     5401 tgctatgcta tactagatgt aaaaaatagg agaaataaaa tgagggaaat ttattctttt
     5461 gaatcaaaaa tctatgcttc cgaagtcgac aagggcgggg catatattat ttttccgtat
     5521 gatataagaa aagaattcgg tgctggaaga gttaaagtcc aagcaacttt tgacaatgtg
     5581 ccgtatgaag gcagtatcgt gaatatgggc gtgaaaaatc cagatggttc agtctgctac
     5641 atccttggac tacgaaaaga tattcgtaaa aacatcggaa aagaaatcgg tgatattgta
     5701 gcagttacag tgaagcaaaa ataaaagagg ttgtgacaaa agacgtgtca caacctcttg
     5761 ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga
     5821 aacccatgtt tacaaaattt ttcttactac tcgaaacgaa gcggctttat cacaacctct
     5881 tgataaacgg cgttcaatca gctgtccctc ggtattagct caaaaacggc aaaatatgag
     5941 aagctatttt tccatttttt tctctcatac tcaggtcata acgctgattt cacaacctct
     6001 tctttcataa tggtttttca ccatctttat aataaactgg ttctttgata tggaaatcta
     6061 ttttttccaa ccaatctaaa atcttgtaac ctaactcttc tgacatcgga aaagaggcgt
     6121 tgatttgtgc attggagata tggtcaacat ggataccgga agtgatcgta caacttcccg
     6181 gaacggcagt tttgatgatc acagctattt tttcggctaa gacatcgtct ttatggaacc
     6241 gaccatcgtg gctagggaaa cgtacagttt gcatctctgt atcacgagaa aatgtagtga
     6301 ctgttccgat atgtggtgta tctccgcctg ttaaagtgat caataagtct ttaccgatta
     6361 ctttaacagc agtttggatc gtgaaaccta gtttggttac ttcaactatc gtttcttttt
     6421 ccatattaat caccctgcca acgttttgaa tgctcattta caattccaaa ttgatccaaa
     6481 agtttcattg tatggtgatt aacaagatct tggatcgttt gaggctgatt ataaaaagca
     6541 ggaattggtg gtatgatttg cacacctagt cgtgctaatt tagtcaagtt ttccaagtga
     6601 atggcattta atggcgtttc acgaggaaca atgactaact ttcgttgctc cttcattgtt
     6661 acatcagcag cacgtgcaat caggttgtcg cctaaaccaa cagagatacc tgcgactgtt
     6721 ttcatacttg ccggtacgat gatcattcca tcggtcaaaa atgatccgct ggcgacagca
     6781 gcacctaaat ctttgtttga ataatggtaa tcaaacaggc tttcgaattc agttagtgat
     6841 aagtcggatt ccagctttaa gttctgtttt gcccatggac tgatgattcc gtgtgtttct
     6901 atcatcggat attcttttag tttcttcact aggttgatgg cataaatggt gcctgaagcg
     6961 ccacttacac caatcacgat ttttttcttc cgttgtgcca attgttccat tttccgcact
     7021 cctttttatg atgtcaaaac agttgcagat cattcattat ttcaaatatt tttcccaatc
     7081 ttctacttct tggaattttg cacgtacaaa atgttctttc atatcgaaag gaactgtacc
     7141 gtcaaagata gtttttgcac tcatcccacg gaaacgaata gagccaggat tatattctgg
     7201 taattctgat gggtccaatg gatggttcct cattcctggg atcacgatga tatctttatc
     7261 tccttggaaa cgagtgttga aagtccaaac tacatcgttc atgtcgaaaa tatcaacatc
     7321 ttcatcgact aaaatgactg tcttcaattc tttgaaggct gataaagcaa gaatagctgc
     7381 ttgacgctga ataccttcat ccgcttcgtt ttctttacgg atttgcatga tggtcatcaa
     7441 tttaccacca cctgcaggag gattgtagac atttactact tttcctggaa tagctttatc
     7501 gaccaaggca agaatacttg cttctgtagg aataccggcc atagatacgt gttcttcact
     7561 gggaccaatc gttgtttgca tgattggatt gtcttttcgg tgagtaactg ctttgacttt
     7621 cactacttgt acagctggat tagcatcccc agtatagcca ggaaattctg gcattgcttt
     7681 tcctgagttc gtgttgatat cttcttgcat tgtttcattt ggcatgattt ctgcttcaat
     7741 gatgaattca gcacgagcaa tacccacttc atcgactgct ataccatcaa cgatttgaat
     7801 aggttcgtta cgcaaagctc ctgcacacca tagttcattg tagccaagtg gagtggtagg
     7861 tggttcgaaa gtcgtgccaa tggcaattgc tggatctaaa ccaatattga tagtgatagg
     7921 cattggttta ttcaaggctt cgaattgttt ttggaagtga ccaatgtggc gtccgccagg
     7981 catgatgtac ataccgatcg tatctttatc ttctagaacc atacgatgga tcgttacatc
     8041 gctcattgtt ttttctggat ctgatcctaa gactacaccc attgtgatgt atggaccagc
     8101 atcttcagga gtatttgtag gtgcaggcaa aatgttgcga atatcgaagt ctgcatcggt
     8161 agctagatga acgacttctt gtgctggagc gtctgctttg tctactttca caggtttgat
     8221 tggattttct acagcgtctt tcaagaaacg accaagtgtt cgataatcat ggtgcaaaat
     8281 ttcacctaca cgtttacgac tagccattgt tccgatgttc acacgtgtgc cggggaaacc
     8341 tttgatattg ttgaaagtca tagctggtcc ttcttgagtc ggacgtttta ctgtaccgcc
     8401 agcaccgata taacggtaaa caccagataa ttctgcttca ggatcgattt caatatctgt
     8461 ttcgtgatat tgacctggaa gtgtttttag ttcttcgatg acttttctta aatcatacgg
     8521 ttgatctgtc atgagagatt cctccttagt tgtctttacc tatctatagt aaaaggtgaa
     8581 aaagaggaaa acaattcaac ttgttcggta aagtattcta caatggaatg gatgcgtttt
     8641 tttgataata gaataaacta tgatatactt atcactaaaa gaaagcgcat acttagtgtt
     8701 ggaggaagga aagtgaaaaa aatgaaggag tcatttccta aagcagtaaa agtggaaaat
     8761 atagcgaata ttttgaaagt tacatttgaa aacggagaag taaaatacgt gaaatctcat
     8821 tggacagaag agataaccga tgccttgcaa tttggaaaaa aaggaagagg aaaaagaaaa
     8881 aatattctcg cactttctcg taatatgtgg attgggactg aagtaacgat tgaagcagat
     8941 ggaacagtat tcataaatgg aaaagacagg tatacgccag aagagttgtg gtacaaagga
     9001 aagaaaagta taccagaatt ataaaaaata attaaaaaaa acagcgcgaa acaaggaaca
     9061 tattacttcc tcgtttcttg ctgttttcta ctatttcagc gctcttatga ctcaaatcta
     9121 aatgtggttg tcacaacctt attctgcacg caaagcgata gcggcgtctt ttttcgctgc
     9181 cattcgtgca ggaagatgac cgccaagcat cgtcaatata gtagagacga tcactagaat
     9241 aatcgcatgg acaggattta gttgagcaac attttttaga tccgtcatgt tgtataagat
     9301 gctattgata gggaacgtag ctagccatgc aataaacaca cctaatatcc cagaagaaat
     9361 acctaaaata cacgtttctg cgtcaaatac gcgagtgatg tcttttttac gtgcacctaa
     9421 agcttttagt acaccgattt cttttgtccg ttcaatcaca gatgtataag taatgatact
     9481 gatcatgatc atacttgtta ctaaagagat ccctgcaaaa gcaataagga cataagtgat
     9541 agcatccatc aatccgccag tcagttcagt catcgttcca gctaaatcag tataaatgat
     9601 tttatcttca tctgatttgc ctttattgta atcatctaga taatccaaga tcttttcttt
     9661 gtcttcaaaa ttgttcggat aaatcatgat acttgaaggc aagctgtctc cacctaaata
     9721 actaagcaat gtttgtttgg cattttcgtc aactttttca gtagttaaga catttacacc
     9781 actgtctctt tgagctttga cgatttctga ttctttgttt tcgttgacga tttgagtcgt
     9841 taattgatcg ctataagcga ttcctggaga taagagattc attgtagaag aagatttcac
     9901 gcgtaggatc cccgagatct tcaattcatc agaagcattc tgatacatcg catcataatc
     9961 tgtattaggg ataaaattcc cagttggaag ttttgtatag taagtattgt tgtttactaa
    10021 tttgaatgtt gtaccaacga tatcatcaaa atcaagtttt tcgtcttctt tcacatcaaa
    10081 tccaagattc ttcaatgcgt tgatattagt attattattg ccgtctacga tcagcacgac
    10141 atctgttgct gaggctggat aacttccggc aagtaaagaa taattgtctt ttaaaaagtt
    10201 ttctttgcta gtgtcaagct gtgtagggaa acttgacact cccacacctg tcattgctga
    10261 catcgtacta gataaagaaa gagattccgt atcaggattt tgattcgaga agctgacagg
    10321 ctgtactttg ccatttacat cacgtaaaag attgattcct gtagtacggg taaatccgat
    10381 attattactt aagttaggat cgatgtctgt tacgtaatcg acgtatgttt gatcaatctt
    10441 attcgtgtgc tgtgctttat cttcttcgct gacttttgca gtaatcgttt tgctgtctgg
    10501 ataatccgct ttgcttgcac ccaatccggc atcgtcacga gtctggttag tcgttacctt
    10561 agaaatagta atcggaaatt tcgccatcgt ttcagcttgc gtgttatcga tttgtttttg
    10621 gaatccagaa gaaagggaca gaacgatcgc gattccgatg atcccaatac tggaagcaaa
    10681 ggaggtcaag aaagtacgtc ctttttttgt tcggatattg ttgaaggaaa gctttaacgc
    10741 tgtccaaaaa ctcatctttg ttctgcgtaa gttaaattga tcatcttttg gtcgttcgat
    10801 gtgaggatgg gaatctgtta agatcttacc atcagaaaat tcaatgatcc gatctgcgta
    10861 ttgatgggcc agttcaggat tatgtgtgac catgatcact aatttttctt tcgataactc
    10921 ttggatcaat tccatgatct ggatacttgt ttctgtatct aacgctccgg ttggttcatc
    10981 gcacaaaagg atatctgggt cattggctag tgctcgagcg atggccactc gctgcatttg
    11041 tccaccagat aactgatttg gctttttatg catatgatct gtcaagccga ctcggtgcaa
    11101 tgcatcttct gcccgttttc gtttttcgtc tttagaaaca ccacttaatg tcatcccaag
    11161 ttcaacgttt tcaataatgc ctagatgacc aatcaagttg taactttgaa aaatgaaacc
    11221 gatagagttg tttcggtagg catcccagtc actgtcctta aagtctttag tagactttcc
    11281 gttgattacc atatcaccgg aatcataatt atctagtcca ccaatcacat tcagcatagt
    11341 cgttttacca gaaccactag gacctaaaat cgctacgaat tctttttgtc tgaaagcgac
    11401 agacacacca tccaatgctt tggtggtggt ttcgccgact ttataatatt tttttatatc
    11461 ttttaattga agcataatca cccttctccc gtattgaaat attcggtata ctaataagta
    11521 tatcagaaca atgatcgtag tatacgaaat aaatgttaac tgaagatgaa ctacaagaaa
    11581 aaatctagaa atttttcaaa aaacagaggg agatcaatga atcgtatttt agtagtagaa
    11641 gatgatccgc aattacagtt gctttaccgt tctgtgttgg aacgagcggg cttttcagtc
    11701 atcaccgcat tgaatggaga ggatgcgctg aaaaaactag aagcgacacg tatagaatta
    11761 atcattacag atatcatgat gccgaaaatg gatggctatg acttgctaga ctcattacgt
    11821 agcaccagat ttgacacacc tgttctaatg attacggcaa aggccgattt tgaggataag
    11881 aaacgcgggt tcaaattagg agcagatgat tatatgacaa aacccatcga tgtcaatgag
    11941 atgatcctac gtgtggaagc attgcttcga cgttcaaaga tcaattacag cagtagcttg
    12001 ataatcggac aaacaaaatt agagctagat gcttatacag tgttacaaga tcagcattcg
    12061 ttgattctgc ctcaaaaaga atttctgctt ctgtataaat tactgtctta tcccaacaag
    12121 atcttcactc gccaacagtt gatggatgat atttggggat tagatacgaa tacagaagaa
    12181 cgaacgatcg atgtacatat caaacgttta aggacccgtt ttgaaaagac ggaagatttt
    12241 caaatcatca cagtacgggg actaggctat aaggcggtta tttaaagatg aaaaaaaagc
    12301 tattttcaca attatggatc tattttgcta gtattgtctt catttcgatc ttagtgacac
    12361 tgctgtgttt tctcggtttt attttcttat tgtctcgaca ttctatctcg cctgctgaat
    12421 cggctggaaa accaacgtta tacccgttat tcgtctttgc ggggttcagt atggtcgtgg
    12481 gtacaggaat atcgattttt gttgggcggc gtatcctgca tccaatcagt gcgctaggga
    12541 caaatatgag tctggtagca acgggagatt tttcgattcg aatggatgaa cagcagaaag
    12601 tagcagaagt acagcaactg tataaagatt tcaacgttat ggtccaagaa ctcaatagta
    12661 tcgagacact gaggaatgac tttgtgtcca gtgtatccca tgaattcaaa acaccactag
    12721 caacaatcca aggatatgtt caattgttac aagccccaaa tctctctgac gaagaacgcc
    12781 aaatattttt atatcggatt atcgaaagta tcacgcagct ttcacaatta acagagaaca
    12841 tcttaaaatt gaacaaactg gaaaaccagc gtatccaact ggaaaagaaa gagtatcgat
    12901 tagatgaaca aatcagagaa gtaatcgttt ttttgcagcc taaatgggag aaagaacagc
    12961 tagaattgga tatcgaactt gctgcagtaa attacacagg caatgaagag tttttgtacc
    13021 aagtgtggct gaatatcatg gataacgcca taaagtacaa tcaaatcaat ggacagattc
    13081 atatcaaact atttgagaca gccacagaga ttgtactgga agtgaccgat tcaggtgtag
    13141 gaatgaatga agagaccaga gatcgaatgt ttgaaaaatt ttatcaagga gatacaagtc
    13201 gtcaaatttc tgggaatggg ctaggacttt cactggttaa aaaaattcta gaactgcatg
    13261 atggacgtat tgattatagc agtatcgaag gagtaggaac gacagcaatg atacggttaa
    13321 gtaaacaata ggaaaaagaa taaaacccct aaaaattcta ttgaaggttt ttaggggttt
    13381 ttagtcgttt ttttgtgatt agggaaatgt tcttacggtt cttttggcat aaaaatcgct
    13441 gctttcttat tgaactacga gaaatgttag aggtgtcatt gaaacaagga catgatgcaa
    13501 gaaagagagc gaaagtcatg cgtcgctctc tttttttggg tggatagatg gacagaaaat
    13561 gtagggctta gttttcttct tgatttaaat actcccaaaa tgtatctgaa gccaaagaat
    13621 gctcaccgac tttacgagta aatgcaagat aactctctgt tgttggagaa atagggagaa
    13681 tcacagtatt ttcttttaat agattcttga ctgttttttc cataacaata gcaatcccca
    13741 tgtcatttgc aatcatattg atgatcaagt cgattcttgc tcctttataa gagatttttg
    13801 gttcaaatcc agcttcgcga cacagatcta gtacaggttg atacaagttc gtagtggggc
    13861 caaggagcaa aaagttctcc tttttcagtt cgcttagatc caatttcttt ttactagcta
    13921 aaggatgatt tttaggtaga actgctacaa aatcgtctgt ttcagtaggc aaccactcaa
    13981 tcgaacttgg tggggcctgg aaacttctag caaagtggat cgtgtcacta tgttcattgg
    14041 cgtcattgta agttaattcg acactttcca cttctttcaa ctggactgta aattcaggat
    14101 ggcgtttcaa aaactcggtg attttggaaa aacctttata attgatcatt gtcggtatcg
    14161 ttaagatatt taatgaaaga ttgttgtctt tcgtataggt ttcaagcact tcttgcatag
    14221 catggtattg ttctacgaca ttttttacat atgggagcaa taagcttcca gcttcggtca
    14281 attcgatttt tctatgggcg cgttcgaata atttgacgtc taattctgtt tctaacgaaa
    14341 gaatctgttt agaaatattt ccttgggtgg tgaattgtcg atcagcagtt ttggtgaaat
    14401 tcattgtttc ggctaaatca agaaaaactt cgaatttatg gatatccatc atattcctcc
    14461 tacttattcc aataaagaat actttaacac aaaaaatgaa ttggtgtaat cttctctgtc
    14521 tgttacaatc tttcttgcag cgaaagcaat aattacagaa atttttctga tttttttcgc
    14581 ttcgtagaaa aagagaggag ttttttgatg ccaactggag ttttgatcaa tgtaggttcc
    14641 gttttgcttg gcggactaat tggcggatta gtaggaaata aattaagtga acattttaaa
    14701 gcacagctga cgatggtttt tggtgtgtgt tcgatgggga tgggaatcta ttccattgcg
    14761 ccgatgaaaa atatgccggc tgttattttt gctttagtga taggaacagc gattggtctg
    14821 attgttcatt taggtaatgg gataaataag ggggctgcgc tgatgcaagt acctatttct
    14881 aaaatcttcc ctagtgaaaa gcttggtatg acccacgatg aatttatcag cacattagtg
    14941 acggtcattg ttctattttg tgcaagtggt acggggatct atggaagttt agattctggg
    15001 atgacaggag acagtacgat tttgatttct aaatctgtcc ttgatttttt cacagcagct
    15061 atttttgcgt gtaatttagg gtatgtcgtt tctgtgatag caattcccca attcattatt
    15121 ttttatattt tgttcttatt ggcaaaattt atttatccat tgactacacc agacatgatc
    15181 ttagatttta aagcttgcgg cggattcttg atggtcgcta ccggttttag aatgatcaac
    15241 gtgaaaatgt tcccagtagc tgacatgata cctgcaatga tcgttatcat gccactgagc
    15301 tggatgtgga caaattggat tatgccactg ttataataaa aatcaaagag aagccatttt
    15361 ttgctgacta aaaaggatca aatcttttag tcagtttttt tgtatttgac aaactgattt
    15421 tgaaactata ttatatgttg tataatatag attgaaataa aatattacac ttgagaatag
    15481 aaggtgacgt aatgcacatc cagattccaa ctgttcttct tgatggtact gtgctagcag
    15541 cattaagcaa ggaagatatg tatgggtatg ccttaacaaa aatcgtacaa gaaagtctct
    15601 ctgtcagtga atctacacta tatccggttt tgagaagatt gaaaaaagaa ggatatcttg
    15661 agacctatga tgaacctttt gaaggcagaa accgtcgata ttactcgttg acaccaatgg
    15721 gaagtcgtca tttagaagaa attattgatg aatggattgt ttttcgagat tctttagacc
    15781 ggttattgga ggagagcaca aatgaatgaa tatttgaatg aagttgtgga tcatcttcaa
    15841 gaaattcctg aagacgatcg ttttgatttg atccagtatt atgaggaata ttttttagat
    15901 tctggaaaaa cattagatca gatcattgat gaatatggta caccaaagca gtttgcctta
    15961 aaattaaaaa tcagttactt ttcagaagaa gaacaagcag aaaacgagtt agtgaaaaat
    16021 ccaaaaagac aaattcgcct gatttggtta atcattatcg gtttatttgc ttctcctgtc
    16081 ctcattccac tagcactggg gtttatattg gttattgggg gactattggt tgctttacta
    16141 gcgctcattt ttagtatcta tgttttatgt atcagtatct taggtgttgg cctattcatg
    16201 atcatctcag gaatcagtgt gtttggacaa tcgattctga gtggattatt ctttatcggt
    16261 gtcggtctgt tcgccacagg aagcgcagta tttatttcgc cactgttgct gaaaggaaca
    16321 aaagggcttt tttatgtcat gatgaacttt gttaaatgga tgggacgacg ttttattaca
    16381 aaaagagatc tgcattcagc gggcgtttga aggaggacac ataaattgag attgaaacat
    16441 tttttgatta ccggtattgg tctgatgctt actggtggcc tcctagcagg tgcaacttat
    16501 acattaggcg ctcaaaagag tctgacttgg gataatggtc ctcggttgat cgatatggtt
    16561 actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca tccatgcaga aaatcaaaca
    16621 gtttcggtca tacgcggatt ggattttgag ataaaaacga cttatgataa gacagaaaag
    16681 ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg cagtcaaaaa atcaacaggt
    16741 gcaatcatta gtttagaaga cacaagtgca caaatcacta ttgttgtccc tgcagatcat
    16801 gaattatctg aattgacaat cgaagggaat aatagtgcaa ttgatatcaa cgaagtatca
    16861 atcaaaaaag tgaatactaa gacacatgat ggctgggtat cgttcaatga atttacagga
    16921 ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat tagatgaggc caatgtggat
    16981 aacttgatcg tcacaggtga aaacagcaac ttccatctag aagacagttt gatcagcaaa
    17041 gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc atgagatgga aaacagtggc
    17101 tatactatcc aaacgtctgg tgaatcttat attgaaaaag actaccagcc aataacaaat
    17161 actttgttag aacaaggaca gaaaaagcta aagatcactg ctaaagacac atcgatcaat
    17221 attacgacgg atcaagagga caatgaaaca gattgatcct gtactcaact ggatatttta
    17281 agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt catcgcttaa cgcacataac
    17341 tatagtaaaa taagaaagaa aatatctaaa aagaaaacag ggtgaaagag tatatgaagg
    17401 tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga aagattacat acttatatca
    17461 ctcaactaag ccaaaccttc tatgatccag acaaagaaaa agtgatggtt aattacccga
    17521 acaattcaga aggtcgccaa ttagagcaag tgtaccatga agttttcaaa catttattga
    17581 cggtgaagaa agagctggat tattattctt taccgattat cgatacagga atattgaagt
    17641 atgatgggaa gaaggaacga tttatcttta aatctgttcg ggaagatttg cctctttcag
    17701 cagggatgga tctagaggtc ttggtagaag actattttac agaagaaaag cactgggttc
    17761 gaacaaaatt ggattattta ccgcaggcag ccggagggac acaggcaagc ggttggtaca
    17821 tcactgaaga caaagagtta gaactagaag gtgtaatggc gcgtattcga aaaaaaaatt
    17881 aaaaaagtaa cattagatat ttattgaagg ataaaggagc gcaggaagta gcatgagaga
    17941 aaaaagagca ttttatatca atggttatgc agggattgtc ggattggtcg tattagcttt
    18001 agttggattg tttttcttct atttaggtat gtggcaagcg aaagtttggg cacttttttt
    18061 aagtattttt ctttggttga tcacgttatt gttgttaagt tcggcaactg tagtcagtcc
    18121 taatcaagca aaagtaattt tgttttttgg acaatattta ggtacgattc gagaaaatgg
    18181 ctttttcctt acgatacctt tggcacaaaa aatgactgtt tcgttgaaag ttcggaattt
    18241 caatagttct gttttaaaag tcaatgattt ggatggaaac cccatcgaaa tctcagcagt
    18301 agtagtcttt aaagtcgttg atacagcaaa ggcattattc gatgtagcgt attatcaaga
    18361 ttttgtagaa atccaaagtg agacagccat tcggcatatc gctagccaat acccgtatga
    18421 tacattcaat gagaatgatt tgacgcttcg aggaaacacg acggcagtgt ctgacgagtt
    18481 gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc gaagtaatcg aaacacgctt
    18541 gaaccattta gcctatgcaa cagaaatcgc tagtgccatg ctccaacgtc aacaagcaaa
    18601 agccattttg tctgctcgac aaaccattgt tgaaggagct gtcacaatca ctcaaatggc
    18661 cttagagcaa atcgaagatg gacaagaaat caatttcacc gatgatcgga aagtccaatt
    18721 gatcaataat ttacttgtct caatcattac agacaaagga acgcaacctg ttatcaatac
    18781 aggcgacatg aaagaccgta agtgacatac tttttgtaag ataggaagaa ggagtttagg
    18841 acggattatt gtcttgaact ctttttttag ttgttttaaa gaaaatgaca aaattgaaag
    18901 aagaatgata gttgattcga aggcagaatt gttcgctttt ttctataatc agtatataaa
    18961 gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg tctacttgtt ttcatcgttg
    19021 tggtacttgc aggggtgaaa atcgcagatt acgtagttat gggtggagaa gaatactatg
    19081 tacaaattac gacagatggt acaaaaacag tttcaaaatc agactctgga caagaaacag
    19141 tcaactatag atatatcctg cctggctata cgaaagaggg taaggaaaaa gaactggact
    19201 tcaagggaca attgactcga ccgcttcgta aaggagcata tttaaaaatc acctggaaca
    19261 aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa agacgtgccg aaaacagcac
    19321 tggaaaaact aattaaaggg tagggaaaga aatgaacaaa atagtcgaag tacagaatgt
    19381 aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa gcattaagcg gcatcagctt
    19441 cgatgtagaa aaaggcgaat ttattgggat catgggcgct tctggttctg gtaagtcgac
    19501 acttttgaat attttatcca cattagataa accaacagat gggcatatcc gaatcaatca
    19561 aaaagatgtg accacattaa aaggaaatca actagcagat tttcgtgcga atgagattgg
    19621 ctttattttc caagatttca atttattaga aagcttgaca gcacaagaaa atatcgctgt
    19681 tcctctttct ttacaaggcg tcagaccaaa agaaatcaag caacgtgtcc aaaaaattgc
    19741 agaacgttta tccatctctc atattttgga aagctatcct tctgagattt ccggtggaca
    19801 aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa ccaacgattc tattagggga
    19861 tgagcctaca ggtgcgctgg attccaaaag tgcccgggat cttttagata cgatggacga
    19921 attgaataca aaagatcatg tatcgattct tcttgtgacg catgatccgt tttctgcaag
    19981 ttattgccaa cgtattttat tcatcaagga tggcggtatc catcaagaag tgaaacgtgg
    20041 agatcaaagc cgtgattcct tctatcgaga aatactatct attcttggaa acttggaaca
    20101 gtagggggag attgactatg ctttggaaat tatcacttac tgggatcaag agccgtttaa
    20161 gagactactt ggtgttattc tcaggattag ttatggcatc tgctattttt tacatgtttg
    20221 aatcaatggc aagcaacgaa gcttttttga aaagtaatac gatcgttgcg gctacagtta
    20281 tcattttccg attcggttct gttttactag ggatcatcac attcgtttat attttatatg
    20341 caaattcctt tttgatgacg atgcggcaaa aagactatgc aatgtttatg atgctgggag
    20401 caaaaggcag aaaaatcgca cagatgattt tttctgaaac attcttagta gggatcgcag
    20461 ctactttagt gggttcagct gtcggtgtag gattgacaag tatcgtccac cgattattag
    20521 tggatcagtt agatctcaat atttcgcatt ttacaccttt tagtattcac gggttgttga
    20581 tcactgtttt gttttttgct gttttgtttt tattagctgc tgttgtcaat gctttttcga
    20641 ttgtcaaaaa accgatttta gcattgattc gtgcggatca gacaccaaca agaatcaagc
    20701 agcataaatt cttgtttttc cttgaagtag tactgggaat tgcttttcta ggctgcggtt
    20761 actatatgat gaaacacgtg gggacctatc aagtctttgg tataggggta gccttagtta
    20821 cgatcgtatt aggaacttat ttcattttcc attctgtgat cattttcttt ttatcattac
    20881 taaagaaatc agaacagatt tcattgaaaa aattgaacaa tttcacattg tcacagttaa
    20941 actttcgtat tcgtgaatat acacaaatgc tctcaatggt cgctatgctg tttgccttgg
    21001 cactgggcgc tttgacggta ggattaggtt ttaaaaatga aattgcagac ttgacagaaa
    21061 acaattctgg atatgatcta gtgatgaata atcctcgggc agaagatcag caaaaaatca
    21121 aagaactttc accaacactt tctgcttctt atactcaaaa agaagatgcg ggaacgattt
    21181 actataatgc aagtgaattt gcggctcaac cattgatcat gataaaacat gaagatggaa
    21241 caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa aatgaatgaa ctgactgtac
    21301 aagaggaact tcgaagctat gaattgccgg aacagcaaga aaaagaaatc aaggtagtca
    21361 gtcaatcaga atttgatcaa ctgaacttgc cacaatccac tttacagcta gttcaggtaa
    21421 aagacttcca agcaaacctg aaaccaatcg aaacacttgt tgctcaaaat aaaatgaaca
    21481 atccatcttt gcaaaaagta gagtacagca accagaaata tgaaatgtat gaagtgtata
    21541 atggtctatt ctctggtttt gaatttatgg gattcttcct aggtttagct ttcttgacca
    21601 tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag caagagtgac attgctcgtt
    21661 atgaaatgct tgaaaagata ggaacacgac gcgggttgct taaacaatcg atccgtagag
    21721 aagtaggcgt gttattctta gcaccaggaa tcttaggtgt agtccatgtc ttattcggat
    21781 tacaaatgtt tggtctattc atgcaaaatc catacaaaga tatttgggtt ccgtttacga
    21841 tcttcttcgt attgtatttc atctactatg tactgacgac atggttatat actggaatcg
    21901 tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa
    21961 aaatagctcc tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    22021 caccgtttgt tcggaaaaag aggttgtgac aatatttttg tcacaacctt tttttcacgg
    22081 tcttttatta gaatatagta aattttttct cgttactcac ggaataagct gttaagaaaa
    22141 cggtttccat gtatactgga atcagtgcaa atgaaagaaa agagggtgca aggaacatgg
    22201 aaatcaatga acaaaatttt ggcgaaaact atcacttgat tacgatcacg aatcaaaatg
    22261 ggacatcttt gtctgtcagt gatcttgggg cacgggttgt tcattttcaa acaataatcg
    22321 ataatgaaaa acgagagttg atcttaggtt ttgatgaagc aaaagaatat ctagaaaaag
    22381 acccttatat cggtgcttca atcggacgta tggctggacg gattacgaat ggcacattca
    22441 aattagatga tgaaacgttc gaagtaaaaa cagacccgga cactggtcat tgtttacatg
    22501 gtggtgcacc tagctttgaa gccaagcagt ggcattatac agtcatcaat ggtgaaaacg
    22561 aagcatcggt cattttctat cagactagcc cagatatgga aaacggattt cctggaactt
    22621 tagatgtaga agtacggtat acgctgacga acgatgatac atggcgagtg acggtccaag
    22681 ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca tgtctatttc aatttaaccg
    22741 gtgatccagc acagtcaatc gacgaacata tactttggtt gaatagccga ttatttgcta
    22801 cgctcaataa agatacaacg cctacaggag aagtagtttc agtaagtggc acagcatttg
    22861 acttccaacg tcctaaaaaa ttaggtgagg tctttgccag tgattttcgc caatcaatga
    22921 tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca atctggttta ctaaatgctg
    22981 cggaattgac aagcccagat gaaaaagtat cgatccaagt aaaaacagat gcttcaggaa
    23041 tcgtgatttt tacagcaaat tttggagaag atggaccgga aatgcgcggg aataaattag
    23101 taaatcatgg cggtatcaca tttgagactc aagaactgcc aggagcggag cgacttgaaa
    23161 gttttggaga tgtcactctg gaacctaacc aagtacgcga gtttgtaacg gaatacaaaa
    23221 taaatataaa gaaatagaat aaattaaaaa gggagtgtga cagtgtcaca tccccttttt
    23281 aatttattct atttctttaa aggcagatca agccagaatg atttgtctaa acttaacaag
    23341 gttagtacag gaacagttgc agctttaact gtacgagtag ttgtttctgg tatctgtctt
    23401 gccgtaaaat tgttccacaa atcccagatt gaagcgacac ggaagatgga agaagcgttg
    23461 ttgacgcgtt ttatcgttga aaattctaat cctgtgattg gcacatcgaa tcgcttagac
    23521 atattgagga tttcagcgtt tgcttcttca gttgttgctg cttctttttt cggttcatca
    23581 tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct
    23641 gtagcataac gtccttccaa atatttggtt gcttctttgt agtcttttgt ttccgacttc
    23701 caagttggtt tgtagaaatc aggattgcca gtcagtccat ttttgatcaa gtcggcatag
    23761 tcttccaaac tttccttgta agaaggatat ttacggaatt gtgcgcggat ttggtagctt
    23821 tgtccggaac tatcttgttc aagtgtattg aaggtcacgc tgcttccttt atatgatcct
    23881 ttgataccaa aaaggttatg atttggttcg cttgataata agctgtttcc agatccactt
    23941 tcaaggatag cttgagccat catgactgat gcgtagattc cttctttttg cccgatttcc
    24001 tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg tttggttttg actgaaatgg
    24061 aacgagccat ctaatccatc taaaatagat gagccgcctg tcagcgaacc tgaagcagca
    24121 ggtaaacctg ctaagtttac tcggacagca aaatcaggcg tgaaatctga aatagaagtt
    24181 actcgaactt tatctcctgg ttgaggcgca tggataaatt gtccgttccc aatatagata
    24241 cctacatggt gagtaacacc ggaagaaggt ccccagaaga cgagatcgcc aggttgggcc
    24301 tgacttactg gaatctgcgt acctgcatat tgctgttcgc ctgtccagcc accgatataa
    24361 tgaccggttg ctttcatata tacgtaataa gtcaaaccag aacagtcaaa agtatttgga
    24421 ccttttgctc cccatacata tggttttcca agatgcttga atgcttctcg aacgattgct
    24481 tgttggacat tcgttgtatt tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa
    24541 tcagaagaag cagaagatgg attctctata ccgctttgat ttgctgtatt tggacgagat
    24601 gaagcattgt tcgttccgcc attcgatgaa ccattgtttg gatggtttgg ttctctctga
    24661 gtatttccat tgttattcga tccagatggg tttgtttgat tcgaagaatt cccgctgttc
    24721 gaattattgt tattattgtt tgaattattt gaaccattat tattgttgtt attacccaca
    24781 tctggtttgt ttggcgtttc cggatctggt gtactaggga cttcttcact aggatcagtt
    24841 ggtttctctg ttgaagtatc cggtccagct ttaaattctg cagtaataga taaattgttc
    24901 gtacctactg taaaggtctt tgcatcataa tttacttctt tggcaccatt aacagcgact
    24961 gaagcaattt gttcaccttc agctggcgta atagtgtaag ttaccttcgt ttccgctaat
    25021 aaaccttcta ccgttccact ttccgtaata tccaaaggtt gctgttcagg ggaatccgct
    25081 gtcgaagcag tcagttggat cttaccaatc aaagaaggag caattgtaag ggaatatgtc
    25141 tcggctggct tttcattttc tgatggatca gccggatcaa tttcagttgg ctcggtcgat
    25201 cctgttgttg agttagttgt tccagttggg tcttctgtaa aagaatccgt tgtttcacta
    25261 gtagctgagc tggatgtagt cgtatctgtt gtgactgttt gatcagctat ttgattatct
    25321 acggcaaata cggtattggt cgacaaaaca gacggagcaa tcagccaagt agcagctagc
    25381 aaacgtatcg tttttttatt tttcataata aactagtcct tttcctttca ttgattttct
    25441 gtcttattca gcattcatta gacatttttt ggaacagata atcgtgtcga aaagcttttt
    25501 cttttcataa atctgtctgt gtattctaaa taaaacaatt tgtaaataag caacgagcat
    25561 tcattcgttt ttctttgacg ttcattttat tgcatagacc acctttgttt ggggataaac
    25621 aagtggggat aaaaatggtt atgcatatgt aaattatacc aaagtttgga aagtatcaca
    25681 aaaatttata aaaataatga tcctaaaaag aaaaagactg aaatatgtga taaacatctc
    25741 atacttcagt ctttttcatt aactattcat aagacattag gtcttcatca aacccgctaa
    25801 cgagagtagt gatcattgct acgactagac taacagccag tcctgctaag taccagaaaa
    25861 aagtaccgga aatgaatgca ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt
    25921 tcacttgggc aaatcccatt acagctccac cagcagctgc accaatgcaa gcagcaacaa
    25981 atggtttttt caatgggatc gcaattccgt aaacagttgg ttcggtcact cccgcaagta
    26041 aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt tgaacgttta cttcgcagaa
    26101 atacacctaa agctgcacca gccattccaa aattggctgc tcctgtcaac ggaacgattc
    26161 ggtcgaaccc ataagcagag atattactga tcatgacagg gtttacagcc cattggatac
    26221 caaatattac caaaatattc caaccaccag cgatgattgc gccactgatc ggtccgttaa
    26281 tatccatcat ccaagtaatt gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc
    26341 cgaataccat taacgtcaaa ggaaccgtga taactaatga aagtaaaggt acaaatacta
    26401 attgcatatt ttcgcgaata tattttttga ggaatttttc aacaaaagaa taaaagaaaa
    26461 tacctaaaat cgccggcatg acagtagacg tatactgcat cagtactaca ggaatcccca
    26521 gaaaatcagt agctgctttg ccactgtctg aaagtaaaga ggtcagattc ggttcaatca
    26581 gtgctgcagc aatgacagcc gcaatatatc cattcacttt aaacgttttg gcagcagaaa
    26641 aagcaagaaa gattggcaga aaataaaaga cggcattcga cgcagcagac aaaatatgat
    26701 aagttccgct tgtttcacta agccagccaa tcgtagtcgc taaaacaacc agacctttta
    26761 atataccaga accggcaaac agcggaatga tcggcgtaaa gatttccgat atcaaaccaa
    26821 atagtttttc gatcggattt agcttttctt tcggagctga atgatcgatt tgacgcgtca
    26881 attccttgta agcaggagcc aatccctgat ccatgattat ttctgcatat ctatcgagta
    26941 aaatgaaata cttaatggaa gggatttgct gttttaattt ttctgcattg gctactggct
    27001 gttttttcaa tttgatacga agccgagtcg cacatggaag gacagaatca atattgtctt
    27061 ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga cagtaccatc tgttcaggat
    27121 tcattgtcgt ttccattaga agaccactcc actcactaat ataatattgg cgtaaggtgt
    27181 catttcgcct gtacgaataa atgcatgaca gttattcaac tcttttttca tttcttcatg
    27241 tgggataaag ctaatcggtg tatctggtaa caataatttg attttttcca atatttccgg
    27301 gttcattgtc tttatttctt ccgccaattc aatcgattgt acctcaagtt cttctaaaac
    27361 gttttctaaa acagataaaa aacttggaag accgtttgag acagccaaat ctattttttc
    27421 agtaccaaag gggacaggca tgcctgcatc cccgatactt aatttatcaa agtgccccat
    27481 ttgtgcgatt actcgtgaaa tatctgaatt gattactttt ccttttttca tgtttgtttc
    27541 ttcctctcat tgttaatttt agactgctga ttgttctaaa gcttcgcggt aagggataga
    27601 aggttgcgca ccataagact gcacagcaac agaagaggct agatttccat aaaggatcgc
    27661 ttctttcaag ttactaaggt ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc
    27721 acctgcggct gttgtgtcaa cggcatccac tttaaaagca gggacgatac cgtgttcttt
    27781 ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg attactgttc caatacctaa
    27841 ttcatggagt ttttcggctg ctgcgaccaa actgttatga tctgtcactc tgattcctgt
    27901 aatgatttct gtttctgttt catttggtat aatgatatct gtcttttcaa gtagttcagt
    27961 aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt gtttttcctg cgtcttttgc
    28021 aatagaaaaa gctaggatcg tcgcatctaa gctactttca aactgagcaa taataaagtc
    28081 actgtttgta atcagttgct tgttttttag gacttcttgt tcatggaaag cattatttgc
    28141 ccctgcatgg atcaagatac tattttctcc tgctgcgtct accataatca ttgcttgtcc
    28201 ggttgtggcg ccttggattt cagctatccc agatgtatcg atgttttctt ttgtcagtag
    28261 atctaataac tgttgtcctt cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc
    28321 gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat
    28381 ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc
    28441 catattaata cttccaatta ctgtaaccgt gttcatttta caaacctcca actatctcat
    28501 ttattttgat gggaaaatta ttttttaaaa cgttttagta aaacgtttta atagttcgat
    28561 aaaaatatag cacaccttga aagctatttc aagggtgcag ttgaaaatct tttttctaat
    28621 cgtacaggta atcttttttc ttcccattct ttttctggga attggatccg atctaacaat
    28681 aacttggcag atgtttgtcc caattcgaag attggttgtg cgatcgttgt cagtttcggc
    28741 ttgatatact cgcacatatc tatattatca tagccaataa tgctgtagtc ttcagggatg
    28801 ctttttccag cttcctctaa tccgcgatat aatccaaaag ctaattcatc atttaaagca
    28861 aacacaccag ttgcagcaga atcaagaagt tctgctgtga tctggtagcc accttgtttt
    28921 gagaactgtg taggtaagag gatcagttgg tcgtgaggga tttggtaaac atcaagagca
    28981 gatttaaacc cctcaatccg agcgtggaca ttttccggtg gattttcagg atagacaagg
    29041 gcgatcgttt gatgtcctag actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa
    29101 tcgtctgttc gtacggcatc actaaatcct tcagattttt tctgatccaa aacaatgaag
    29161 ggccggcctt gttttttcaa attttcgttg attgcatctg tagagacagc agaagtcgcg
    29221 ataatgaatc catccacacc tcgacgtgtg agctcggcta gatattcgat ttctttttga
    29281 tgatcagaat cagcattaca taatatcgtg acgaaattct gcttatacaa gatatcctca
    29341 attcctcgca tcagtgtact gaagaatgga ttcgtgatat caggaacgag tacaccaatt
    29401 gttttcgttt ccttggtaat catttgtcta gcaaaatagt caggctgata gcctaattct
    29461 tctttggcag ctaaaacttt ctgaacagtt ttcggactga atttctccgt atttccattt
    29521 aatatcaagg agacagttgt gattgaaacg ccggaaagtt tagcgacttc tttgattgtg
    29581 atcttttttt tcaccatgtt catactccta tctaaatcct gtggttattt tcccatgaaa
    29641 tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta tttgctttgt tggatagctt
    29701 cgattatttg tttcgttgag cgtactcgtg cagttttcgg aaatatcttt tttatgaaca
    29761 attgatggga ttctttgtca cgatcactgc aagcatcttc agcaagcacg atatggtacc
    29821 cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt tgttgtcgct acaccgctta
    29881 aaatgatagt ttcgattcca cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag
    29941 cacttggatt gtattttgtg atagtcaaca tattatctgt atctttcaaa gaatccttca
    30001 aaagtaaatc agtgaattct ggaggtacag aagcagtcat tccacccata tcagtttgtt
    30061 gactcaaata agggaaagtg gagatgtcta cgttcactaa aactttcaag gcagcggtac
    30121 gtttcaatgt tcttactaac aaatcattgt ttgtcagaat agatgctgca gtgtgtggag
    30181 agagtgtacc gttatttacg atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg
    30241 ataaatcaaa aatcatattt tcctttaaat cattcatgat gttccttctt tcaaataaca
    30301 atgtttttta tgtaagtaaa tcataaatcg tttatttgtc aactaaatta aaggattcct
    30361 ttctttagtc tagagaaatt cagtggtaaa ataaataagt gtgagaaaac gcattcgatg
    30421 catagatata gcgagaaata tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta
    30481 aaaaatgaat acaaacgata caaaatggga gaaacgacca ttacagcaaa tgacgatatt
    30541 tctttttcaa ttgaaaaagg cgaattagtg attatacttg gtccaagtgg agcaggaaag
    30601 tcaacgattc ttaatatttt aggtgggatg gatacacctg atgaaggtca gatcattatt
    30661 gatgacacag atattgcgca attttcagat aaacagctta ctgcttaccg acgaacagat
    30721 gtagggttcg ttttccaatt ctacaatctc gttcctaatc taacagcaaa agaaaatgtg
    30781 gaattggcaa cggaagtttc tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg
    30841 ggcctagctc atcgattgaa taactttccg tcccaattat caggaggcga acagcagagg
    30901 gtttcaattg ctcgtgcact agctaaaaat cccaaacttc ttttatgtga tgaaccgaca
    30961 ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga
    31021 caacacggca ataccgtttt gatcattaca cataattctg ctttagcgcc catcgctgat
    31081 agagtcattc acattaatga tgctaaagta aggtctgttg aattaaatga tcatccttca
    31141 tccattgatg agattgtatg gtagggggat tttatgaaaa caagaacata tcttaaagca
    31201 ggttttcgcg aaattcggca atctaaagga cgattcattg caatcatcct tattgtcatg
    31261 ctaggaacac ttttatttgt aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg
    31321 gatcactatg ttggtacagc aggactttct gatttgcaaa tcgtttctac tggtggcttg
    31381 acacaaaaag atattgctca agcagaaaag atcccagatg caaaagtaga aacaggaaaa
    31441 cagatctatt attccaatac tggcaaaaac gaagtgattc aaatattttc ttataacaaa
    31501 aataacaagc agaacaagct acaattaact gatggaaaac tcccggaaaa accaaaccag
    31561 cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa tcggtgatat ttatcagatt
    31621 gattcagatg aattgaaaga aaaagagtat accatcacgg gctttgtccg ttctccattg
    31681 ttcatcaaca atttggagag aggatacgcg aacgtcggaa atggtagcgt cgattatttt
    31741 gtctatttac ctgaatccgt ttttaaaaca gatatttatt ctgtcttata tcttgatttt
    31801 acaaacgtaa agaatttgaa tacgtacagc aaagcttaca aagataaaat ggagcaaaat
    31861 caagaaaagg cagaaaagta tttacaagat cgacccgaag aacgcttaga agagctgaaa
    31921 aaatcggcca acgaagaatt agaaccggca cagcaaaaaa tagcggatgg caaagcacaa
    31981 acaacgcaag caagaacacg gttagaagat gctaaaaaac agttagcgca acagtcagca
    32041 actatccagc agcttcctga agcgcaacga acaatcgctg ttcaaacatt atctcaacaa
    32101 gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga aagaacgtga actggcctct
    32161 gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa atttaactaa accatcatac
    32221 ctatacaatg aacgttcaga aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt
    32281 atcgctgcga ttgccaatgt ttttcctgtt ttctttttct ttattgccgc attgatcacc
    32341 tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg
    32401 ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca
    32461 gggataggta ttgttttagg aacgatatta ggaacgaatc ttttgccaag gatcattttt
    32521 gagctgtcta acgagcggta tgatattggg agtgcgctta ttttttatga ctggccgccg
    32581 attttacaag cagctgccgc attcttcatt gccgcatttg gcgctgcaat gatcgtttta
    32641 ttcaaagatt taagagaacg gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg
    32701 aaacgcattt ttcttgaata catcactccg ctgtggtcac gccttagctt taatcaaaaa
    32761 atcagttacc gaaatctctt ccgatataag tcaagaatgc ttatggcgat tatcggaatt
    32821 gctggatgtg ccgggctgat ggttgcaggc gtgggattga aagattctct aagttctgtt
    32881 tcagccaaac aatttggacc gatcattgac tatcaagcaa tcgttacatt ggattcatcc
    32941 acggaagata gtcgtcaaaa agcagcggat atgctgcaga agcaagaaaa agtaaatgag
    33001 atcctagcta ttcatactca aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt
    33061 ctgacagtga tcgtaccaag taaaacagct gatttggatc gttttatcca tttaactgat
    33121 cttgaagaga acccacgctc cttgcctaaa acaggaatcg ctattacgca aaaagtagca
    33181 gaactatttg acttatctac aggagatcat atttcgcttt atgatgaaga tcaaaaagaa
    33241 tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag gtagctttat ttatatgtcc
    33301 cctgaatatt atgcttcagt tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt
    33361 acagataaga tgacaacaaa agaagaagaa atgctttccg agaaactgct tgccacagac
    33421 gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg
    33481 aacttagact cggttgtctt aatctttgtc gtattatcag gactgctggc ttttatcgtc
    33541 ttgtataatc taactaatat caatatttcc gagcgtgtac gcgaattatc tacaataaaa
    33601 gtccttggat tttttgataa agaagtcacg atgtacattg ttcgagaaaa tatcatcttt
    33661 actcttttag gaatcatcgg aggatttggt gtaggatata tattgactga cttcatcttg
    33721 caacaagcgt ctatggagaa tgtcatcttt ccattagtga tcacatggtg ggcttatgtc
    33781 ttatcagcag gattaactat cgtatttacg gttattgtga tgattgttac ccatttcaaa
    33841 ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga
    33901 aatcagcgtt ttgagctaat aacgagggtc agctgctaat tgaacaccat gaatcagagg
    33961 ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc aaataaaatt agctcctcat
    34021 gttttacatt tgagaagact gattgccgta gataaaagct tttgaacacc atttatagaa
    34081 gaaacaagaa tattttgcta ttttaaacaa gaggatagac aaatacatgt ctgatctctt
    34141 gttttttatt gggcaacaga cctcttagaa tgtataaaga taaattttac aaacgctttc
    34201 ggttcagtta taataataaa tgataacttg tatatataag ttgtggtagg taataaatca
    34261 gttgaatgga ggtaaatatc catgacattt aaagacaaag tcatttacca aatctatccc
    34321 aaatcttaca aagacagtaa tggtgatgga attggagatt taaaaggaat caaagaaaaa
    34381 ctcccgtatc tgaatgatct aggcatcgac atgatttggc taaaccctat ttatccaagc
    34441 cctcaaaaag acaacggtta tgatatctct gattacacag ccatcgatcc tatctttggt
    34501 acgatggagg aattcgaaca gctaatagcc gaagcaaaga aataccggat agaaatcatg
    34561 ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa agcactagca
    34621 ggggaccagc attatcagga cttcttcatt ttaagagaag agccaacgga ttgggtatca
    34681 aaatttggtg gaagtgcttg gtcgccattt ggagatacgg ggaaatatta tttgcactta
    34741 tatgatcgga cacaagctga cttgaactgg cggaaccaag cagtaagaga ggaactattc
    34801 aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat
    34861 gttatcggta aagacgaaca attgaaaaat aataaagaaa atgatggcaa acctgaatat
    34921 acggacaaac caatcaccca tacgtatcta aaagaattga accaagcaac gtttgggacg
    34981 gattccacta tcatcacagt cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg
    35041 tatacagaac ctagtagaga agaattatca atggtcttta atttccatca tttgaaagtc
    35101 gattatgaaa atgggaataa atggtctact cctccatttg attttgcaga attaaagcga
    35161 ctatttcata catggggaga aaagatgagt gagggtaacg gctggaatgc tctatttttt
    35221 aataatcatg atcagccacg tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa
    35281 caaggagcag agatgctagc aaccatgatc catttgaacc gtgggacgcc ctatatttat
    35341 atgggagaag aaatcggcat gatcgaccca gactattcat cgatttccga ctatattgac
    35401 gtagaaagtt tgaatgctta taagttactc cttgaacaag gattcacaga aaaagaagcg
    35461 tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa cacccatgca atggtcagac
    35521 acagaacaag caggatttac tacagggaaa ccttggttaa aagttggagg caagctagaa
    35581 gagattaatg tagaaaaaga actatcttct gaagattcta ttttgtccta ttacaagaaa
    35641 ttgattcggc tgagaaaaac atatccaatc gttgcacaag gagattatca tgcttatgaa
    35701 gcaaatcatc cggaagttta tggctttctt cgccaatttg aaggacagca gctgcttgta
    35761 ttaacaaatt tctatgataa agatacaagg atcgccatcc cagaaggatt cacagaagcc
    35821 aatattctta tcgataatta ttccgatact gttcttcatc aaagtctgga gctaaaacca
    35881 taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag
    35941 ctattctcgt aagatcgacg ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa
    36001 gagttttcat actctccttt atcgccataa aatttattgt agcgttgttt gaatttacgg
    36061 aaaataaaag taacgagtaa tttgactaac gtatagatag gaataccaaa aataacgccc
    36121 acaattccca ttagatcacc catgacaagt aaaacgatta agatcgtaat cggatggatc
    36181 tgtaaacgat cacccattac tcgaggaatc accaagtcac cgtggagaag ttgtacggca
    36241 aaccagacaa tgacaaattt gacagccata aaagtagagt cttggaatgc aatgatcaaa
    36301 cctggaaaga aggcgataaa cggcccaata tatggaatga tacataacaa cccagcagaa
    36361 atagctaaaa cactagcaaa ttctaatccg atcagtaaga aacaaaccca gtagactgct
    36421 ccaaggataa atgaagcaat gatttgcccc tttagaaatg aacccaattg ttgattagca
    36481 atctttgtca aatttttaaa atctgtacga aatgctggtg ggacaatcgc taaaactgac
    36541 tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga caattggacc agtaaataaa
    36601 gtaataaaaa tagctgtaac agttgaaaaa atgttgctga tccgttgtgc gccactttcc
    36661 caataatttc caataaatcc agctatatca gacgtcacgt cgtcaataga agcaaaaaat
    36721 tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa
    36781 tcttgaaaca aatctggcaa actactgatc aatgtctttg cctgttcttc aagaaaagga
    36841 ataagccata tgcttccaag ccccaataaa aataaaataa tcacataaac gataatgact
    36901 gacaagttcc tcgaaaggtg cttgctcaaa catttgatga ctggttccaa aagataatac
    36961 aagatcagtg caaacaataa tgggggcaag atagtagcga taatgattcc gacaggcttg
    37021 aatataaacg agatctgatg gaaaaaataa atgatcagcg caagtaagat aactattcct
    37081 aaaacatagt aagaggtttt tcctcctaaa aagcggatca aacgattcgt tgtatctgtt
    37141 tcttttttca ctctttttcc tcctttatct ataacttcat tatagacaaa agcgggtata
    37201 aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa tcattcatat aaactttctc
    37261 atcaattaaa ataagaaaac gcttgacagt tcttttaacc atatgataaa ttaaatcctg
    37321 ttgctaaaac gtaactatta cggtttgcga aaagcgaatc ggaacattca aatgaaagaa
    37381 gatgagaaga ggagtgtcat aaatgaaacg attgataggt atattggcca tgctggtttt
    37441 agcaggtatg ttaactgctt gcggggcaag tgggaaagcc gaggacagta aagaaaaatt
    37501 gagcgtgatg acgacctttt atcccatgta tgattttaca aaagcaatcg tcggggatga
    37561 aggagaagta gagttattga ttccagcagg gactgattcc cacgattatg aaccttctgc
    37621 taaagacatg gcaaagatcc aagatacaga tatctttgtt tataatgatg aaaacatgga
    37681 aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc aatgtccata cgataaaagc
    37741 gacagaaggt atgcttttac ttccgggaag tgaagaagga catgatcatg accatgaaca
    37801 cggggaagaa ggccatacac atgagctaga cccacatgta tggctagctc caagtttagc
    37861 aatcaagcag gtagcaaaca tacgtgatca gctgattgaa gcttacccag aaaaacagga
    37921 agtatggaca aaaaatgcag cagcctatac cgagaaactt caagcactcc atcagcttta
    37981 tcaagaaacc tttaagcaag caaaacaaag atcttttgtc acgcaacatg ctgcattcaa
    38041 ctatctagcg ttagaatatg gattgaatca agtatcgatt gctggtcttt cttctagtga
    38101 agaaccgtct gcggcgagaa ttgctgaact gaaacacttt gttaaagaac atggaatcaa
    38161 ctatatttat tttgaagaaa acgccaaaga tagtattgcc agaacgttag caaacgaagc
    38221 aggtgtatct ttagaagtat tgaatccttt ggaaggattg acgaatgaac aaatagaaaa
    38281 tggagaaaac tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga
    38341 gacagaaaac ccgctagaag atactttgac ccccaaaaaa gaaaagagcg tctacaacgg
    38401 ttattttgaa gacgaggatg tacaagatcg ctctctttca gattgggcag gaacttggca
    38461 aacagtagat acgtatgtag aagatgggac ttttgatcct gtttttgaat ataaagaaaa
    38521 attgaatcag gataagacag cacaagaata taaagaatat tatacgaagg gctatcaaac
    38581 agatattgct tcaatcaaga tcagtgagaa agagatggca tttacgttta aggatggaac
    38641 aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa
    38701 ccgtggcgta aggtatctat tcgaagcaga agataaggaa agtggcgctt ttagatatat
    38761 tcaattcagt gatcacacga ttgcaccggc tgactccgaa cattttcatt tatatgcagg
    38821 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
    38881 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
    38941 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
    39001 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
    39061 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
    39121 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
    39181 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
    39241 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
    39301 tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt
    39361 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
    39421 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
    39481 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
    39541 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
    39601 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
    39661 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
    39721 catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc
    39781 aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa
    39841 gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt
    39901 taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt
    39961 gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc
    40021 caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg
    40081 ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga
    40141 cagtaaacta cggatctcag gaactttcat attatccaac atacttagat tggccggaaa
    40201 aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc
    40261 taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt
    40321 gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct
    40381 gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc aataataaac
    40441 caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac
    40501 atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat
    40561 caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga
    40621 tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata
    40681 ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat cgtcgatgct
    40741 tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg
    40801 ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc
    40861 agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac
    40921 tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta
    40981 aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt
    41041 accaaattat agcacaatta gcgtattatt tttttaattt ttcagggatt ttgaattcag
    41101 aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa
    41161 tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc
    41221 aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag
    41281 aaaaataaag taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg
    41341 ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt
    41401 aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga
    41461 tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat
    41521 tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg
    41581 attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt
    41641 ccaagattat tcaataaaac ttctaaggca aattttactt ggacatacac catatcttct
    41701 tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc
    41761 tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca
    41821 taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa
    41881 gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt
    41941 gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt
    42001 cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta
    42061 tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt
    42121 gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt
    42181 gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag
    42241 gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca
    42301 tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa
    42361 aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg
    42421 gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa
    42481 atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca
    42541 ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt
    42601 tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt
    42661 ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg
    42721 caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata
    42781 gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt
    42841 atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac
    42901 cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa
    42961 gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac
    43021 tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg
    43081 atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa
    43141 tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta
    43201 caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt
    43261 gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag
    43321 atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg
    43381 ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac
    43441 agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa
    43501 atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc
    43561 cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag
    43621 ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat
    43681 ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc
    43741 gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc
    43801 ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa
    43861 atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt
    43921 ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac
    43981 gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc
    44041 cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta aaatcatatc
    44101 gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag acaacgaaac
    44161 tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc
    44221 agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt
    44281 ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg
    44341 cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa
    44401 acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa
    44461 aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg
    44521 gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg
    44581 aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag
    44641 tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt
    44701 ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag
    44761 gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta
    44821 aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact
    44881 attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg
    44941 attccattga taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg
    45001 aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc
    45061 tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc
    45121 tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga
    45181 caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat
    45241 tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc
    45301 ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga
    45361 tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag
    45421 gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac
    45481 aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat
    45541 cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac
    45601 tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc
    45661 aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc
    45721 aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact
    45781 tttctcctaa atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt
    45841 taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc
    45901 atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa
    45961 tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa
    46021 gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga
    46081 ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag
    46141 gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg
    46201 gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca gtgacgacga
    46261 tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc
    46321 tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa
    46381 ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt
    46441 ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcgact
    46501 tatttttcga agaagctact t
//
LOCUS       DAGCXG010000014        46003 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGCXG010000014 DAGCXG010000000
VERSION     DAGCXG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.014"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(275..641)
                     /gene="ssrA"
                     /locus_tag="MB022_000770"
     tmRNA           complement(275..641)
                     /gene="ssrA"
                     /locus_tag="MB022_000770"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(812..1585)
                     /locus_tag="MB022_000771"
     CDS             complement(812..1585)
                     /locus_tag="MB022_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4465791.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1729..2079
                     /locus_tag="MB022_000772"
     CDS             1729..2079
                     /locus_tag="MB022_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBT4465792.1"
                     /translation="MIFFSIGILFLIIGGLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIELLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2060..2776
                     /locus_tag="MB022_000773"
     CDS             2060..2776
                     /locus_tag="MB022_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4465793.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDESGVA"
     gene            2776..4224
                     /locus_tag="MB022_000774"
     CDS             2776..4224
                     /locus_tag="MB022_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4465794.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4294..4803)
                     /locus_tag="MB022_000775"
     CDS             complement(4294..4803)
                     /locus_tag="MB022_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465795.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENHGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4793..5518)
                     /locus_tag="MB022_000776"
     CDS             complement(4793..5518)
                     /locus_tag="MB022_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465796.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5741..7861)
                     /locus_tag="MB022_000777"
     CDS             complement(5741..7861)
                     /locus_tag="MB022_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4465797.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8091..9269
                     /locus_tag="MB022_000778"
     CDS             8091..9269
                     /locus_tag="MB022_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBT4465798.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLFQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSIAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9285..10043
                     /gene="aroD"
                     /locus_tag="MB022_000779"
     CDS             9285..10043
                     /gene="aroD"
                     /locus_tag="MB022_000779"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBT4465799.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGANIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10066..10551
                     /gene="ybaK"
                     /locus_tag="MB022_000780"
     CDS             10066..10551
                     /gene="ybaK"
                     /locus_tag="MB022_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBT4465800.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     KRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10620..11873)
                     /locus_tag="MB022_000781"
     CDS             complement(10620..11873)
                     /locus_tag="MB022_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4465801.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDDPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11990..13339
                     /gene="gdhA"
                     /locus_tag="MB022_000782"
     CDS             11990..13339
                     /gene="gdhA"
                     /locus_tag="MB022_000782"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBT4465802.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13451..14797
                     /locus_tag="MB022_000783"
     CDS             13451..14797
                     /locus_tag="MB022_000783"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBT4465803.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15105..15491)
                     /locus_tag="MB022_000784"
     CDS             complement(15105..15491)
                     /locus_tag="MB022_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4465804.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15759..15908)
                     /locus_tag="MB022_000785"
     CDS             complement(15759..15908)
                     /locus_tag="MB022_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465805.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            16370..16582
                     /locus_tag="MB022_000786"
     CDS             16370..16582
                     /locus_tag="MB022_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465806.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(16860..18659)
                     /locus_tag="MB022_000787"
     CDS             complement(16860..18659)
                     /locus_tag="MB022_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4465807.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQALNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(18783..19379)
                     /locus_tag="MB022_000788"
     CDS             complement(18783..19379)
                     /locus_tag="MB022_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4465808.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            19729..20073
                     /locus_tag="MB022_000789"
     CDS             19729..20073
                     /locus_tag="MB022_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBT4465809.1"
                     /translation="MAEKQTAGRDQLGDFAPKFSELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            20074..20493
                     /locus_tag="MB022_000790"
     CDS             20074..20493
                     /locus_tag="MB022_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBT4465810.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(20585..20935)
                     /locus_tag="MB022_000791"
     CDS             complement(20585..20935)
                     /locus_tag="MB022_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465811.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(20901..22232)
                     /locus_tag="MB022_000792"
     CDS             complement(20901..22232)
                     /locus_tag="MB022_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBT4465812.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIATLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            22488..23054
                     /locus_tag="MB022_000793"
     CDS             22488..23054
                     /locus_tag="MB022_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4465813.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDKYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            23199..23286
                     /locus_tag="MB022_000794"
     tRNA            23199..23286
                     /locus_tag="MB022_000794"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23231..23233,aa:Ser,seq:cga)
     gene            23625..24599
                     /gene="bsh"
                     /locus_tag="MB022_000795"
     CDS             23625..24599
                     /gene="bsh"
                     /locus_tag="MB022_000795"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4465814.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVANYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            24924..26819
                     /gene="asnB"
                     /locus_tag="MB022_000796"
     CDS             24924..26819
                     /gene="asnB"
                     /locus_tag="MB022_000796"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBT4465815.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(27013..27459)
                     /locus_tag="MB022_000797"
     CDS             complement(27013..27459)
                     /locus_tag="MB022_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBT4465816.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDSRAIPIA
                     IDQAKEWYENASESERRTFEKEKNPSKSDKHDTNPRAGKLLDSDVIVEYAEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLVIYKKDGTKEKEIDY"
     gene            27689..27838
                     /gene="rpmG"
                     /locus_tag="MB022_000798"
     CDS             27689..27838
                     /gene="rpmG"
                     /locus_tag="MB022_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4465817.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            27926..28444
                     /locus_tag="MB022_000799"
     CDS             27926..28444
                     /locus_tag="MB022_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4465818.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFTLIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(28521..29477)
                     /locus_tag="MB022_000800"
     CDS             complement(28521..29477)
                     /locus_tag="MB022_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4465819.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQNDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            29643..30449
                     /locus_tag="MB022_000801"
     CDS             29643..30449
                     /locus_tag="MB022_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4465820.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            30446..31435
                     /locus_tag="MB022_000802"
     CDS             30446..31435
                     /locus_tag="MB022_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4465821.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            31432..32436
                     /locus_tag="MB022_000803"
     CDS             31432..32436
                     /locus_tag="MB022_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4465822.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFVLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            32568..33110
                     /locus_tag="MB022_000804"
     CDS             32568..33110
                     /locus_tag="MB022_000804"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBT4465823.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            33130..33828
                     /locus_tag="MB022_000805"
     CDS             33130..33828
                     /locus_tag="MB022_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBT4465824.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            33849..34061
                     /locus_tag="MB022_000806"
     CDS             33849..34061
                     /locus_tag="MB022_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBT4465825.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            34061..35023
                     /locus_tag="MB022_000807"
     CDS             34061..35023
                     /locus_tag="MB022_000807"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBT4465826.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            35041..35436
                     /locus_tag="MB022_000808"
     CDS             35041..35436
                     /locus_tag="MB022_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4465827.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            35599..35964
                     /locus_tag="MB022_000809"
     CDS             35599..35964
                     /locus_tag="MB022_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBT4465828.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            35961..37772
                     /locus_tag="MB022_000810"
     CDS             35961..37772
                     /locus_tag="MB022_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBT4465829.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLIEPPLDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRLFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAAARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(37834..38001)
                     /locus_tag="MB022_000811"
     CDS             complement(37834..38001)
                     /locus_tag="MB022_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBT4465830.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            38250..39239
                     /gene="galE"
                     /locus_tag="MB022_000812"
     CDS             38250..39239
                     /gene="galE"
                     /locus_tag="MB022_000812"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBT4465831.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            39251..40735
                     /gene="galT"
                     /locus_tag="MB022_000813"
     CDS             39251..40735
                     /gene="galT"
                     /locus_tag="MB022_000813"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBT4465832.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            40759..41739
                     /locus_tag="MB022_000814"
     CDS             40759..41739
                     /locus_tag="MB022_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4465833.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            41930..42652
                     /gene="tsaB"
                     /locus_tag="MB022_000815"
     CDS             41930..42652
                     /gene="tsaB"
                     /locus_tag="MB022_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBT4465834.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            42636..43178
                     /gene="rimI"
                     /locus_tag="MB022_000816"
     CDS             42636..43178
                     /gene="rimI"
                     /locus_tag="MB022_000816"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4465835.1"
                     /translation="MLKKFRAYVRSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            43185..43649
                     /gene="rimI"
                     /locus_tag="MB022_000817"
     CDS             43185..43649
                     /gene="rimI"
                     /locus_tag="MB022_000817"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4465836.1"
                     /translation="MICRKKDFDEAYLAECVWVISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            43646..44662
                     /gene="tsaD"
                     /locus_tag="MB022_000818"
     CDS             43646..44662
                     /gene="tsaD"
                     /locus_tag="MB022_000818"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBT4465837.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLRKGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(45234..45935)
                     /locus_tag="MB022_000819"
     CDS             complement(45234..45935)
                     /locus_tag="MB022_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4465838.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT        15038 a         8028 c         9361 g        13576 t
ORIGIN      
        1 tgaaggaaca tgcttaaacc ataattgtgt ttttccttcc atcagcataa aagttcacct
       61 caaaaagagt gtacgaacaa aagttctttt tgtaaacctt aaaatagatt ttcctgaata
      121 cgtattgggg atttctaaat gtttgaatgt ttttttgaat gcaaaatcaa tgcaaaaaat
      181 tttgtatttc tctacttttc tctccttgac caaaataaaa aaacgttgat ttaacaacgt
      241 ttttcataca tgtttatctg tctcaaccat acatatatgg agacggcggg agtcgaaccc
      301 gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca
      361 ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa
      421 cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg
      481 ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt
      541 tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc
      601 aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac
      661 gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga
      721 atcatatccc attatttata taataactgc ccctttctta agaaaaaata agcgattttc
      781 ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt
      841 ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt
      901 cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg
      961 gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt
     1021 tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc
     1081 gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga
     1141 tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg
     1201 aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct
     1261 gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac
     1321 attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac
     1381 cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt
     1441 ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt
     1501 ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga
     1561 aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa taagtttatc
     1621 ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca
     1681 gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc
     1741 agtattggta tcttattctt aataattggc ggtctttttc ggattctgcc ctcaaaagga
     1801 aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg
     1861 ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc
     1921 ggctattatt taaagacatc tgggcatacg aattattttc tgattgaatt gcttctgcta
     1981 gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg
     2041 gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga
     2101 aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa
     2161 agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct
     2221 gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac
     2281 tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga
     2341 gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat
     2401 gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag cagtttcaga
     2461 tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta
     2521 tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt
     2581 agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta
     2641 tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat
     2701 cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga
     2761 atctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttttg cggaatggtc
     2821 gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa
     2881 gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc
     2941 tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg
     3001 acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt
     3061 gtcatttatc cgactaatac agctttactg aattttacct taacagctga tcaatggaaa
     3121 aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca
     3181 caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt
     3241 gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg
     3301 ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca
     3361 ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat
     3421 tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc
     3481 aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt
     3541 cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac
     3601 ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt
     3661 aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat
     3721 gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca
     3781 ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca
     3841 ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg
     3901 gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta
     3961 gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa
     4021 caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca
     4081 aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa
     4141 ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct
     4201 gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc
     4261 tataccgctt gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat
     4321 ataaggactt tgagcaatag tagcatcatg agtaaagagt aagataccat gattttcttc
     4381 atgagctaaa taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc
     4441 aaatgtatct tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag
     4501 ctttactttt tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat
     4561 atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa
     4621 caggacatgg tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc
     4681 aaaattatcc agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg
     4741 tttttcgaga ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt
     4801 cattacgtcc aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac
     4861 taagaatgag acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat
     4921 ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa
     4981 ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta
     5041 agacaagaaa attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc
     5101 acaataaaac caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa
     5161 ggaataattt ggttttagaa agaccagtca ggcgccaatt gatcaattcc tgcttgtttt
     5221 gcctgactga atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga
     5281 atatgccttt tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg
     5341 cagaagcgcc aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat
     5401 ggtaaagagt caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga
     5461 gtattgtctg ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac
     5521 ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt
     5581 gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg
     5641 ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag
     5701 cgacaataat tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg
     5761 tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt
     5821 tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt
     5881 tctctaagcc attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg
     5941 tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa
     6001 ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt
     6061 caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat
     6121 caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact
     6181 gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt
     6241 gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag
     6301 ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta
     6361 attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga
     6421 tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg
     6481 tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta
     6541 acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa
     6601 tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa
     6661 atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag
     6721 aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac
     6781 cagcgttccc atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg
     6841 cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa
     6901 acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga
     6961 aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa
     7021 cttcatgctg gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc
     7081 tttctaaatg gatataaatc acattttttc ctttagcgag cccgaacaaa tcgtcatttg
     7141 gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt
     7201 ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca
     7261 agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat
     7321 cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa
     7381 aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa
     7441 cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg
     7501 cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga
     7561 agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat
     7621 aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa
     7681 gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt
     7741 cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga
     7801 ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca
     7861 agataatctt cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag
     7921 ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt
     7981 ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt
     8041 cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa
     8101 aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag
     8161 accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc
     8221 aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc
     8281 agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa
     8341 aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag
     8401 gagacgggat cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt
     8461 ataacgaaac gttatatgca catcgtgaat tattgtttca agcttttcgt gaaatctacc
     8521 cggaaatcgt aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc
     8581 agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat
     8641 ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac
     8701 ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata
     8761 ccggcgcctt ttctattgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc
     8821 tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg
     8881 atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac
     8941 aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag
     9001 tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag
     9061 aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa
     9121 caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc
     9181 gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt
     9241 ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg
     9301 gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag
     9361 aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc
     9421 gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta
     9481 tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg
     9541 gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag
     9601 gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga
     9661 tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa
     9721 caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccaata
     9781 tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga
     9841 ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg
     9901 gtaaaatttc tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa
     9961 gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc
    10021 aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa
    10081 atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac
    10141 gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa
    10201 agccaatctc gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt
    10261 gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat
    10321 aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata caaacgtggt
    10381 ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa
    10441 agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct
    10501 caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt
    10561 ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttatacat
    10621 tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc cgctgtgata
    10681 ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata cagaccatca
    10741 atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc tccgccagtt
    10801 acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc tttgcatagt
    10861 tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg atccatttca
    10921 agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca agcattgacc
    10981 aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc ttgtttggtt
    11041 tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc taattcttta
    11101 ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt
    11161 aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg taaagtgatg
    11221 tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acgggtcatc cgatatcaag
    11281 ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac
    11341 ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa gataatggca
    11401 ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc
    11461 aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag acgattgagc
    11521 aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc atgatcctct
    11581 tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt attccattgc
    11641 gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa atcatcaaca
    11701 ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt ttttcctgct
    11761 tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc
    11821 atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc cattccctca
    11881 cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt ttgagccaaa
    11941 agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga tgacaaacgc
    12001 aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga
    12061 atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg acactcatcc
    12121 cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag ttattcaatt
    12181 tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt accgtatcca
    12241 atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta gtgtcaatct
    12301 aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa ccggtctgcc
    12361 aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg ataatgagat
    12421 catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc catcgattga
    12481 tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt tcggcgaata
    12541 taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg atttttgggg
    12601 aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca aacatctatt
    12661 gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt
    12721 agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta catgttcaga
    12781 ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga aagaagtcaa
    12841 agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg ctatttatca
    12901 tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc catgtgctac
    12961 tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga tcaaaatcgt
    13021 agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc atgaagcagg
    13081 cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat ctgctttaga
    13141 aatgagtcag aacgcccagc gattgacttg ggaaagagag caagtggatg agcagctgga
    13201 tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt acggcgacga
    13261 aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca aagcaatggc
    13321 agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc attttgttta
    13381 tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa acctataagg
    13441 aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga
    13501 acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat tacgggaagg
    13561 tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact atgacaaaga
    13621 agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg aagtattagt
    13681 tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact tcttgaatca
    13741 cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct tctttgccgg
    13801 aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg atcgcgactt
    13861 ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg cgttccgtgt
    13921 attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac gtatctacgc
    13981 tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag gttgggaaac
    14041 tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg ttggtttgtt
    14101 accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg ctgatgcaag
    14161 caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg cagcaatgcg
    14221 caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg aaccaggaat
    14281 gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa
    14341 aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag gacaattcat
    14401 ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc
    14461 aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc aaggaaaaga
    14521 agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata cagacggtga
    14581 cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg gttatacaat
    14641 gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca acccattcga
    14701 ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca aaccaggctt
    14761 tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt cgctagattt
    14821 aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc atttaggtac
    14881 tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt tgtcacagtc
    14941 ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta ggactttaag
    15001 aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa tcagtaagca
    15061 aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga taggcctaat
    15121 ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc tatgatccgc
    15181 ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc attggcagag
    15241 tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc agcataagca
    15301 tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt gatatcagtt
    15361 tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata agtagactga
    15421 tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat caaccctaaa
    15481 atttttttca tgaaagatct ccttttttat ttttattata ttaaatatta tacagataat
    15541 aacggaatat tatcatcgaa acagctaaca aaattgtcat aaaaaacgag atggttgata
    15601 ttagaacttg tgtttttgtt taggtctaga gataaagaga gacaatttcc aaatatatgt
    15661 aatgaataca aaaagacgtt tgtcgcggat taccgttaca aacgtctttt attgattttc
    15721 cgagatctca tcagtcgcca tttggaatgg ttcactcctt atctagattc gtttaatgct
    15781 cggactgctt gttcgattgt aataccaaac gcaacgttat tttcgttttg tgcatcaatc
    15841 gccataaaac ggttagaagt tgtatcaaaa gctacgcggt attgcatatt tgtttttgta
    15901 aacgtcataa aattcatctc ctttgtaatg taatttagtt cttgaaagtt tcctttaaac
    15961 tttcgaactt ataccaagtt tagcatcgta cgttttttga tgcaattaaa aagcatttat
    16021 cattttcctt atttattttt tcttaaatgg tttagctatt ttcttttaat ccgataagta
    16081 gcccaatcat caaggttaca aatactattg cttgaatata aatcatatgc tcacctcttt
    16141 ataaataact tagcaatata aagaaaaaaa cgcacgaaca acataggaat ataaacaaac
    16201 gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt atctaaaaag cagaaagata
    16261 ctcaaaataa aaaataaaaa aatgaataaa aacgttttca aatacaaggt aatttgttat
    16321 tataaaataa agaaaaaaca aaataaaaat aaagaaaaga gggaatgaaa tggtaaaagc
    16381 agaagtagga agatcttata tatgcaaacc gatcggagta tctcatgaac taatcggctg
    16441 tattgaacgt acatatatca acacggctgt cgtgtatgtt gaaagttatg accaacgaga
    16501 ctatcaactt atcaatgaaa gcaagtatcg catgctcgta aaattctgtg atattagcgc
    16561 acctgctgaa ttggtttctt aaacagtatt tttgagaatt cgcaagaact cccttataat
    16621 ataaataaag acagaaggga gcgagaagat atgccaaaaa gaaaaagttc agggaaaatg
    16681 ggaattattc ttgcagtgat ctctgcaatt cctgctattt tagctattgt tcgtgcgatg
    16741 aaggaatcaa gagaaagtta agcacacatt atgagcgtca aacaaaaaac gcatatacct
    16801 gcggtggcag gttatgcgtt ttttgttgta taaagacttt tttcattaac gatttttgat
    16861 tagccgactc ccaaaacaaa atttagcagt ttatctgaat aagtgatttc ttcatctgtt
    16921 tgtttcacac tttcatttag tagtcccatc tcatcggtta tgataccatc cacaccataa
    16981 aacatcatgc gactcatggc atctgaatcg ttgattgtcc atacatatac ttcttttccg
    17041 tcttggtggg cagaattaat gaagttacgg ttgatcgtac tatattccat tgtcagaaaa
    17101 tcagcaggcg tcacaggggg cccaacaata ttgaaaggta agatgtagcc cacataaaag
    17161 tttggttctg cttcttttaa atctgaaacg gtttggtacg tcaaactttg caaaatatga
    17221 tggttctcta agataacttt gcggtattta tggacaaatc gttcgacaag gtcatccgaa
    17281 tcttttttag aggtcttgat ctcaataagt aatttttggt ttagcttatg tgctttttcc
    17341 aaataagaat caaaagaaca aacaggggct tgcactccgt tttcagtaac tgtaagattt
    17401 gtcaattctt tcaatgtaag atcctgtggt gttccattta cccctgttaa gtgacgaaga
    17461 tttgcatcat gcatcactac gaaatgacca tcttttgttt cctgaacatc catctctaca
    17521 taatcaggat gatagtctct gcttgttttt tcaagtgctt ctaaagtatt ttgcgcccca
    17581 ttttgggtac tcacacctct gtgagagaca gtcactggtt ttgtctgaac agctgaagtc
    17641 agataatcca tattgtaaag acagacgcca ataccgaaaa aaactgcaaa aagagtcaaa
    17701 ccagtgtttt tcagcgcaga gaaacgcaag ttgagggctt gtgggataaa ccacttaggg
    17761 atctcaggca agaagccttc gtcatccatg aaatcaacaa tgatataaaa aatccctacg
    17821 gtagacatca cgatattgaa taataaaatc ccttgtaaaa gcgtcatagc aaacacagcg
    17881 ctgatcaatg catagtctgg gaaaaattgt tcaaccatac tttgtgccaa aataacaacg
    17941 atatatccta aagaagaaag gagtaaaatc gtaccgccaa tcacaataaa ttggcccgtg
    18001 atggccaaaa gacgcgattt ggtcaatgac cagctttccc gaatggctgc tcgaaaagga
    18061 cgatctcgta gaatcatttc cggtaaagca aaaatcagtc gaatcccaat atagccaagg
    18121 aaaatataaa cgagtaaaaa ggaactgacg atgatccaac gattagcaaa aataaaatcc
    18181 ataatgaaag cggggatttt tatttttgac agtagatcag aattaaaact taatccgctg
    18241 atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa aagtaatggg tctcactttc
    18301 ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag agattggttc tttcttttta
    18361 ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat aaactaacag taaaatcgtc
    18421 atcaaaacta cgattaacga gagtagaacc ccaggatgtt gagaaaaaat tgaaggaata
    18481 ttgtcataag acaaatagtc aattgcccct cggtgtaaaa taaaacgggt cgtactgctc
    18541 aataagggta aacaaataaa taaaataaag ccatgcatca gcaagacatc acggaaataa
    18601 gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc tacgtttcaa ataattcatc
    18661 ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa aaaggaaagg gtgcagtgtg
    18721 ttttgcagac cctttcccag atttaacatt gtcaatcaat gactaatttt acaaaataaa
    18781 tattatccaa gaagaccact tagatattga cctccataaa tcaaggccat actatataag
    18841 aaaatcgaca gtggtttagc aatcaaaatg attagtgtat atcgtttcac tgacatgttt
    18901 gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa ctggaagaaa aatagctaga
    18961 gcaaacagct tctcaaaacg gttttgatta tccagccagc caatatattt gttatatgtc
    19021 ttatcgctaa tcatggaaag tatgaatggc tttccataac gacgccctag cagaaaaatg
    19081 ataatcgaac caatgcagat tccaatatag ttatagataa atccagcata agggccaaat
    19141 atcaggacac cagctgcagt actgatccct ccaggaatga ttggaataac tacttgcagg
    19201 atttggatga agacaaaaat aatcggtcct aaaatgatgg aatcacctac taatcctctt
    19261 aacgcattca aatctttgaa aacacctaaa ttaataaaat aaatcgtcag cgcgattgat
    19321 aggcctaatc cgataatgga tatgaaattt atcagttttc gagaggcagc aatattcatt
    19381 attcatcaac tccttagagg ttctttgtta actatacttt actagaaaag caactaactg
    19441 cgccattttt tgatatgaca gaaaacaaac aattttgtaa gttcatcata agtataaaag
    19501 attgatctca aaaaaacatc caactaaaga cttatttaat aacttgtttt ttttaaaata
    19561 aaaaaagcgt tagcaactat gcttaaaaag catatcgact gaataaacta agcattggac
    19621 aggctgaaag actacattta aaatgaaatg cgaagaaaca atagaaaata aagttgcctt
    19681 agaaccatag ttgaatcacc taacagtcgt gaagggagaa taagagaaat ggcagaaaaa
    19741 caaaccgcag gacgagatca attaggagat tttgcaccaa aattttcaga attgaatgat
    19801 gatgttttat ttggacaagt atggtccaga gaagaattat ctgctcatca gcgtagtatc
    19861 atcaccatat ctgcattaat ttcaggagga aattttgaac aattgccagc tcatctgaag
    19921 attggcaagc aaaatggcat tactagtgaa gaaatagcag aaatcattac ccatttagct
    19981 ttttatgtag gatggccaaa agcttggtca gcttttagtt tagcaaaaga aatctattta
    20041 gaagaaaata gcgaagataa gaaagaggcg taaatgatga caaaacacga agaaatacac
    20101 aatggcgtga tttttccagc aggtgaaaaa aacgaagctt acgctcaagt atttaccggg
    20161 caaagctatc tgaatatgtt agtgaatgat cccgaagttt ctgttggtgt aggtaatgta
    20221 acttttgaac caggttgtcg caatcactgg catgtccata cagacggcta tcaaatctta
    20281 cttgtgaccg gaggagaagg ctggtatcag gaagaaggaa agccagcaag acgattaatg
    20341 gctggagatg tcgtcgtaac gcacaaaggg attaaacatt ggcatggtgc gacaaaagac
    20401 agttggtttg aacatttggc gatcactgct ggtaattcag agtttttaga acctgtaaca
    20461 gatgaagtgt atgaaggatt ggaaaatcaa taatgtcaag cttgaacagc tcctgaaagt
    20521 agattttaat cgaactttca ggagctgctt ttttgattca gtcttttata aatagaaaat
    20581 agggttaata ataggaatga gaagcacgtc gaaagatctt aatattgata atcggctggg
    20641 catacatgat ttccacgtca tcaatcaaac tacctaacag atgataatct tcccgatcat
    20701 gtcggggatt acctaatagt tcttgatagt agatttccat actagggtcc cgaggattgt
    20761 tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta ccctgtatct
    20821 ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt atttcatggg
    20881 aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt tccaagtgtc
    20941 tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat acctgcaacg
    21001 aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt gctgacgtta
    21061 tggacaagag caatatgtag cacaccagct attacaccgt aaaaactgcc gtaaaaaccg
    21121 cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt actggtgatc
    21181 tcatgaaaag ttccaaacaa agtagcaata aatattccga ctagtatcgg aaaactgttt
    21241 tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc gatgacaggt
    21301 ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc aactaagccc
    21361 atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt caattttcca
    21421 gatgaatcga aaatttctcg aatccccgaa agccgaaagg aattataata gaaaccaatt
    21481 ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg atattcattg
    21541 aaaaccaatg tgttttcgac gattgaatag ctgaataacc tcaaaacagc agtaaaagtc
    21601 atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg atggaaaact
    21661 aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc taaattcccc
    21721 aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact aacgaccggt
    21781 gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt attgacaaat
    21841 acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt tttgccaaaa
    21901 aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa aatcaccttg
    21961 tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat gaatgcgctt
    22021 ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt aagaatagtt
    22081 agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa aagaaaaaaa
    22141 acacctgtaa aaccaagata aaaatacttt cttttctgtg aaacgattcg tttttgagga
    22201 tgaatataaa taacgtcagt ttcgttttcc attacacacc ttctttttca ttttctttaa
    22261 ctataacttg aaatgaaaga tttggacaac tatcatttag gtcaaaacta aaaatgtaag
    22321 ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta aagaagactg
    22381 aaaaaaaatt taaaatataa cgttttgagt aaaatgtttg caaaaaatgc taaaaatagg
    22441 gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg agaagaaaag
    22501 tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag gaagggttta
    22561 aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag ccgatttatc
    22621 tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc tttgaacaac
    22681 taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat ctcggtttga
    22741 actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac attgatgaac
    22801 atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa atagttaaag
    22861 aagatgaaag atacaatcaa ttatcggata aatatgtcga tgctctgcat actggtacat
    22921 gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat ccaacagaag
    22981 aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta gataatcctg
    23041 aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt ggctgcaaac
    23101 tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt agaaataggc
    23161 ttgacatttc gtggaaaaag tttaaaaata gtacttacgg aaggttggca gagtggtaat
    23221 gcaccggact cgaaatccgg cgaaccggct aataccggcg cgcaggttca aatcctgtac
    23281 cttccttata tcaacgatta gaggttgtaa agaagctgtc ctgcatcaag taatatgaac
    23341 ggccaaacaa aaatagcttt tcctattttt tcgtttggtt ggacttatta ccgcagatgc
    23401 aagcttttga ataccgtttg ttcggaataa ggggctgtga caaggctttt gtcacagccc
    23461 cttatttttg ttgtactttt agcctacaat attttttgat tccccctgag tactattttt
    23521 tataaatgct agtaaatcaa tcttctttgg cctttttttt gataaaacaa aatactagat
    23581 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
    23641 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
    23701 aagtagtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
    23761 ataaacatta tgcaatgatt gggatagcag ctggggtagc caactatcca ctctattatg
    23821 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
    23881 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
    23941 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
    24001 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
    24061 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
    24121 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
    24181 cgctatcaaa tggaacacca gaaaatcatt tttcaaacca gatcagcttg aatgtttata
    24241 gtcgtgggat gggaggattg ggcttacctg gagatctctc ttctgtttct cgatttgtaa
    24301 aagccacatt tactaaaatg aatgctgcat caggtgattc agaatctgaa agtatcagtc
    24361 aattttttca tatactaggc tcagtcgagc aacaaaaagg agtatgcgat actggagaag
    24421 gaaaatacga atatacgatt tattcttctt gctgtaacgt tgataaaggg atttattatt
    24481 atcgtacgta tgaagacagt cagattactg ctgtaaatat gaataaagaa gacttggatc
    24541 gtcatgaatt ggtcagttat ccagtcataa aagagcaaca gataaactat gtcaattaat
    24601 taatgaatag atccgtttgg tatcatattt tctgatacag tcataattag acacgatctt
    24661 taggtcgtgt ctttttttgc tggttttttt ggaaatagca tccactaata tttttttaac
    24721 acgaagagaa taagatagcc tcaaaaaaga aaacatggta aattagatat tgggacttta
    24781 ttttctgtaa aaacagaaac tttcatcttt ttttttcacg tgacgaaact gtaagagaga
    24841 aaatattcgt ccgctattta ttctatgata agatgcatat gaatgaaata aacagtaatt
    24901 ttatattagg atgtgaaagt taaatgtgcg gtattgtagg ttttgttaac gacaaggaca
    24961 acaaaaaaac aattattaat acaatgatgg atcgaatcgt tcaccgtgga cctaatagtt
    25021 caggagaata tatcgataaa catgttgctt taggattcag aagattaagt attattgact
    25081 tagagggcgg tactcagcct atttacaatg aagatcggac aaagatcatt atttttaatg
    25141 gcgaaatcta taattaccag cctttgagag aagaattgat tgcagctggc cacgtttttc
    25201 aaacacatgc agatacagaa gtccttttgc atggttacga agaatgggga acagaattat
    25261 tgcaaaagat ccgcggaatg tttgcttttg ctatttggga taatgaaaag aatgagttat
    25321 tcggtgcaag agaccacttt gggatcaaac catattatta tgcagaaatg aacggaacat
    25381 ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc tgattttaac aaagaattga
    25441 acaaagaagc gttgaaacct tatatgactt tccaatattc tccattaaac ggcgaaacat
    25501 tcttcaaaga tgtctatcgt ttgcctgaag gccattatta tacgtataaa gatggaaaac
    25561 tagacattca gcaatattgg gatgcagatt ttgaaacaaa agaaacgcat tcccgacaag
    25621 aatggatcga aaaaatcgac gaaaccgttc aagcttctat tgaagcacat acggtcagtg
    25681 atgtagaagt tggttctttc ttatccagcg gggttgattc cagttatgta acctctgtat
    25741 tgaaaccaga tcattctttc tcaatcgggt ttgatgataa aacctataat gaagcgattg
    25801 aagccagaaa actaaccgaa ctattggact tagataatac agcagcagtg atcgacggag
    25861 acatgtcgtt caaagcattc cctttgatcc agtatcactt agatgaacct gattcaaatc
    25921 cttcttgcgt acctttgtac tttttagcaa atcttgcttc acaaagcgtg cgggtagtcc
    25981 aatctggaga aggggcggat gaactgtttg cgggatacca aacgtatgga ttccatacga
    26041 attcaaaatt catccgtgtg attgctcaag gattgaaaaa attaccaaaa ggaacacgct
    26101 ataatttagg acgtaaaatc ggaaaaatga aaaatttcca tggacggatc catttatatg
    26161 aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc tcgagtgttc gaagaatcag
    26221 aagcatcaga agtgttgaca ccaaaatatc aaacagcacc atctgtggat gaaatcatga
    26281 ctgttcatta cgagaaaaca gaaggaatta aagatgaagt caataagatg caatatgtcg
    26341 atcttcatca atggatgcca aaagatatct tgttgaaggc agataaactt tctatggcaa
    26401 gttctttgga agtccgagta cctttattgg atatcgaagt catgaaattg gctcaacaaa
    26461 taccaagtaa gttcttattg aatcaaaaca acacaaaaga tattttcaga caagctgcga
    26521 acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt aggattccct gtgccaatca
    26581 aagcttggtt gaaagaagaa cacggctacg aacaagtcaa agcattattc gaagcagatt
    26641 ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt gctagatgac catcacgaag
    26701 gacgtaaaga agagcaaaga aaaatctgga caatcttcag tttcttgaca tggtataaag
    26761 tctactttgt agatgaaacg attccgcaag cagaagcaat cgactatgtg actgtttgat
    26821 gaaaatcaaa tatcaataaa aaataggttt tctcctaccg tttaacaccg agtgataaga
    26881 aacaatttca aaaaacagct cttcccgttt tatagatatt atgacttgtg gttcggagtt
    26941 ggcagaggaa caccattgtt tcgaacataa ggggttgtga cacgattttt gtcacaaccc
    27001 ctttacatat gttcaataat cgatttcttt ttcttttgtc ccatcttttt tataaatgac
    27061 caaagttgat tctttgtttt gtgctacttg ctttcctttt tcgatcgctt ctttctttgt
    27121 atcaaaatgg ttgctagcct tttttgctcc tttagatttc acgatccatt gttcttcggc
    27181 atattctacg atcacatctg aatcaagcaa ttttccggca cgaggattcg tatcatgttt
    27241 atccgactta cttggatttt tttctttttc gaatgttcgt ctttctgatt cactagcatt
    27301 ttcataccac tcttttgctt gatcaatggc aattgggatc gcacggctat cagggtaacc
    27361 ctcgtctaat agagcatttg cgatgtcgat cgcctttttt cttgtcagtt tatctaagtt
    27421 tttcattgat gcaggaaagt ccttcatgtt ccatggcata ttagtatccc cctttattta
    27481 aaaaaacata gtctctataa acaagtgtaa gggtatttat acaaaaagaa aaagattttg
    27541 tctgagaagt aaaaccgttg tatttatcgt caagtagttt tacttaacaa aatcgtgaaa
    27601 aacactagca atctggaaaa aatcatggta agataatcaa gtctgagagt catcttagag
    27661 gattcttgta aaaatgggag ggaaaatcat gcgcgtaaac atcactttag agtgtacttc
    27721 ttgtaaagaa cgtaactatc taacaagcaa aaacaaacgt aacaatcctg atcgtttgga
    27781 aaaacaaaaa tattgcccac gcgaacgcaa agttactttg caccgtgaaa caaaataatg
    27841 tttttaaagt tctcaatttc ttgctggtca aagaattgag acttttttta tctcaaaaca
    27901 tctaaaatca taaggagcgg ataatatgtt tatttccatg tgggcacaag ataaaaatgg
    27961 tctgatcggc aaaaatggtt tgttaccatg gcgattgcca aatgatatgc gtttctttcg
    28021 cgaacataca atggatcgaa ttttagtgat gggaagaaag acctatgaag gaatggggga
    28081 tctttcactt ccttatcgcc atatcatcgt gttgacaacg caaccaaatt ttcgcacgaa
    28141 agaaaatgcg gaagtaatgc attcgatcga tgaacttttg tcgtatagtc aaacaatctc
    28201 agaagatatc tatgtttcgg gtggtagtcg gatttttcaa gaacttcttc cttacactgg
    28261 tcggatctgg cgaacattga tcgatagcac gtttgaaggc gatacgtata ttgggaatat
    28321 tgatttttca gacttcaccc ttatagagga gtatgagggg ataacggatc aagaaaatct
    28381 atatgcccat cgttttcaaa aatgggaaag agtaaaaaat agggttgctt caaaagaaga
    28441 ataaaatcta tgcgtctagt ttgttaataa gaacaaaagc atcttacgac taatcgtgaa
    28501 gatgcttttt ttagttaaaa tcaatttagt ttttctttga tatcattgac catttcctga
    28561 ctggcaatag gtccgttata tagccagcta gattcaggtc cgaattgcag tacatgattg
    28621 tctgtcactg ctttaagatt cttccagctt ggttcattga acatggcagc atctttgtca
    28681 ctgttcacaa ggatgatgta gtcagcatct aattcagcta gcttttcaga ggaaacagca
    28741 gaccagtcag aagtggcttt tttggagacc tcttccacta gatcaggaac accaaattcc
    28801 agatcgtcgt atacgattct tccacttgac cgatttttgc tgaccataaa ggctgagttg
    28861 ttggtcaccc acaacactgc agctgtcttg ttctcgattt tatctgctag ttcttctcga
    28921 gttttcttga tggatttttg ataatcggaa atgatttttt ctgcctcttt ttcttttgcc
    28981 aaagctttgc cgacatcttt tagttgttct tcccatgtga catctgttcc gttttttaca
    29041 acataagttg gcgcgatttt gctatattgt tcgtatttcc cgccttcgac agtagcggaa
    29101 gaagaaatca acagcaaatc aggttcaaag cttaacactt tctcataagg aaggtcataa
    29161 gaaatcgttg ggacatcctt taaatcattt tgcagataat cttgaatgga accactgcca
    29221 acagtccact gggcgatagg tttttcccct agtgcaatca gatagtcttc taagtaactg
    29281 ccgatgatcc gtttgggcgc gtttggaatc tctactttat gtccaagtgt atctgtgact
    29341 gtatgagtac tattggatga agtcgtcgaa ttggcagtcg tagctgtttc tttatctgaa
    29401 gagggggagc aagcgctcag tactccagcc agcagcaata tgctagataa tgtgaatact
    29461 tttgttttta atttcatttc tttcctcctg acataattga gaatctttct cattggttat
    29521 tattcctgat atttcgaacg ctgtcaaccg cttcatttgg ctatagatat gaaagtagcg
    29581 cttgacgaaa tgttgaaaca gcgtcataat tgagaatgat tttcagcgaa aggatgggtg
    29641 aaatgacgag attaagagca gctgacattg cagccggcta cgaaaacaaa aaaatcattg
    29701 aaaacatgtc tgttacgatt cctgaaggga aaatcactag tttgattggg ccaaatggaa
    29761 gtggaaaatc tactttattg aaaagtttta cccgtatact acctgtgaaa tcaggaaaga
    29821 tcattgtcaa taactctgat atctctacat acagttcaaa agaacttgca aaaaaaattg
    29881 cattattagc acaaactagt gaacacccta caggtatgac agtagaagaa gtagtatctt
    29941 acggacgata cccttatcaa aaactgttca gtggcatgga cacgcaagat catgaagcaa
    30001 tcgagtgggc gttggatgct acaggtctag ctggatttcg tatgcgggat atcgcaacgc
    30061 tgtctggtgg acaaacccag cgtgtctgga tcgctatggc gcttgctcag gaaacagata
    30121 tcttgatctt ggatgaaccg acaacatttt tagatccggc acaccaatta gagatcctac
    30181 agttattgac tgaaatcaat caaatgaaac aaacaacgat cttgatgtcg attcatgaca
    30241 ttaatcatgc ttcacgtttt tcagattatt tattagcaat gaaaaacggt aagttgatca
    30301 cagaaggtac acctgaagaa gtgatcacaa aagagtgtat gaaaaaaata tttgagattg
    30361 atgcaacgat tgtcacgctt ccaagaacaa ataaaccagt ttttctaacc tatgatttgt
    30421 tgccaaaaga gcaggaggat caattatgag gactatgaag aaaaatagac tcctgctttt
    30481 ggtctgtttt gttcttttga ttattttttt tgttgctctt tcaaatggcg cagcgactgt
    30541 gggaaaagca gatttttggc aggcattgtt tgattttgat agatcaaaac aaagtcacca
    30601 aatcatacga aatattcgtt tgcctcgtgt ggcgggagca tttcttgtag ggagttgttt
    30661 tgcattgagc ggtgcattga tgcaaggagt gacaagaaat cctttagcag attcaggctt
    30721 actaggaatc aacgcgggtg cttcgttggc aatggccctt agttttgctt tttggcctaa
    30781 agcttctgat ttttctgtgt ttctaatgtc attggcaggt tcttttgctg taactttgct
    30841 cgtatttggt tttttgcagt tttcgaaaat gggcatgaat cctttacagc tgattttagc
    30901 tggcgtagct attagttctt tttttactgc tatcagccaa gcgcttaccc aattctttaa
    30961 tctgcagcag gatcttgctt tttggtttgt aggcggagct gccaatatca catggaaaca
    31021 attagcagta ttgtttcctt tatatatact tgctgttttg gtttcttttt cattaggaaa
    31081 agcaatatct ttgattcatt tgagtgaaac acatgcatta gctcttggaa gtcatcctaa
    31141 acgaacgcgg ttgattgttt tctgtctagt cgcagtgttg gcagccatag cagtttcgtt
    31201 agtcggtcca gtctccttta ttggactgtt ggttccgcca gttgttcgcg gaatggttgg
    31261 aacggactat cgttatgttc tacctgtctc ttttttagtt ggcggtggat tggttatgtt
    31321 ggcggatttt acagcgagga tgattaatcc cccttttgaa acaccttttg gattagtcat
    31381 tgcattgatt ggcgttccgt ttttactgat acaagtaagg agggaacaac ggtgaaaaaa
    31441 atatattgga cgttttattt cttgcttttt gtacttgtca tcctgtctat aggtagttta
    31501 atgattggta cgccttttat ttacctggat cagctttttg ccacgttcat agggaaagaa
    31561 tcatcttctt tggcgctgat tgttagagaa tttcgagttc ctcgattact gatcagttta
    31621 ttagcaggag cttgtcttgg cgtttctggt tatatcttgc aaggagtaac aagaaatgaa
    31681 ttggcagatt ctagtatatt gggcattaat gccggagcag gatttttagt gatgctttat
    31741 ttaggtttct tttcgcacta ttcgctgccg tttctattgc tagttgtagc ctttctaggt
    31801 ggtgttttag ctgcttgctg tgtctatctt gcggcctatg atcgaaatgg ctttttgggt
    31861 atgaatcgaa tcttattgtc cggtattgcc gttaatgccg gcctttctgc attaacattg
    31921 ctatgcacga ttcaattatc taaggaaaat tatggatttg tgaatgcatg gttagcggga
    31981 tcaatctggg gtgcgagctg gccttatgtt tgggcattgc ttccatgggc ggttatctta
    32041 attccgctag gaggacttta tgcacagagg attggattgc tatcatttgg tcaagaacgc
    32101 gcgcaaggat taggccttaa cgtggctaaa agtcaaaaaa tccttttgtt gttagctgtt
    32161 gctttggcaa gcggtagtgt agcggtgaca ggtagtttat cttttgtagg actactagct
    32221 cctcatgctg cgaagctagt cgtacgtaaa gaaaataact ggacctttgt tctgagcggt
    32281 ttgttcggtt ccatttttgt gcttagtgca gatatggttg ctagagtgat tctaccaagt
    32341 ggggaagtac ctacaggaat tttgattgcc ctttttggtg cgccatattt tctgtatgca
    32401 ctttttatca gacaaaaaca cgcgctgaac agttgaaatt cattaaaaaa ggaaggccaa
    32461 tcatttctag gtcttctttt tattctattt atgcatttat ttgaaagttc gattgctatt
    32521 tttctttttt tcctatacca ttttatgaga gaaggaaggg tgagtttttg gaccaattga
    32581 aagtaagatt acgccggaaa ggaatcgaaa agctgaaaaa tctggccaaa agtggcaaga
    32641 aaaaggaaaa agaacagcga atctataaac agctattcgc cagcgagtac tggaagtcag
    32701 caaaagtgat agggacgact ttatcgaatg aatttgaact ggatacacag ccggttatcc
    32761 aacgagcaga aaaagaacaa aagaaaatag ttgtacctaa aactttgccg aaacgtcaga
    32821 tggcgtttta tgaaatggat gaacagtccg ttcttgtacg ttcgagtttt ggtgttcttg
    32881 agccacaatc agagaagctc tatttgccgg agatgatcga tctcctcatt gtaccaggta
    32941 ttatctacaa tagagccggt tatcgaatcg gttttggcgg tggttattac gaccggtatt
    33001 taaataattt tcataaaaaa acatgtagcc ttgtttttcg tgaacaattg aatgaacaat
    33061 ggcaaccgga gagctttgat cagaagatcc aacaattgtt tattgactaa aatgaaatgg
    33121 gaggttttca tgaattatca acagcaaata aaattgcgtg catggttaag acgaccgtta
    33181 tggacctatg cttttttagg gatacagaca gcggtcttcc tcatgatgga attatttccg
    33241 cgacttgaga tcccttatta tgccggaatg tatggtcctt atcttgttca cttcaatgaa
    33301 tggtggcgtt tagtgacacc aatcttcatc cattttggac tgatgcattt cgtaatgaac
    33361 tcactgatac tgtattttat ggggcagcaa atcgaggcaa tctatggcca ttggcgtttt
    33421 ttcttgattt atatgttcag cgggataatg ggaaatacag caagctttgc ttttaacgaa
    33481 gcgaatgttc tgtctggtgg tgcgagtact tcgatattcg gtttgttcgg tgcgttgttt
    33541 atcttagggt ttcattttaa acataaccca gcgatccagc agctagtcag acattttctt
    33601 ttatttattg tgttgacatt tgtttttggt cttctggata cctctgtaga tgtttgggga
    33661 catgtcggtg ggctaattgg cggattagtg ttagggaatg ttttgggtct accgaaacaa
    33721 cagacaagct attcgataca ccaaaggatt ctttctacac tagtttttgt atttcttttt
    33781 gtaatatgta ttttactggg tttaaaaaaa tatggtttac ttgtataata tgaaggaagt
    33841 gcttaaagat ggagagctta tatgatgtac agcagctgtt caaaaaattt ggcatcttta
    33901 tctatgtggg tgcacgcatc tatgacattg aattaatgac tatcgaattg cgtaatctgt
    33961 atgatagccg tttgattgat catgatacat acatgacagc gtggcgcata ttaaaaagag
    34021 agcaccgaat tgaagaaagc cgaaagaaag gaaatttgta atggacaaga agattattgg
    34081 gattgattta ggtggaacaa cagcaaaatt tgcaatttta acaccagaag gagagattca
    34141 acaaaaatgg agcatcgata ccaatatttt ggatgatggt aagcatatcg ttcctgaaat
    34201 cattgaatca atcaaccatc gtttgaattt atataatatg aaagccgaag actttatcgg
    34261 aatcggcatg ggtaccccag gaagcgtgga cagcgaagct ggaacggtta ttggtgcgta
    34321 taacttaaac tggacagaag ttcaatttgt gaaaaaactg atcgaggcag gaacaggaat
    34381 caaattcgct atcgataatg atgcgaatgt tgctgcttta ggtgaacgat ggaaaggtgc
    34441 tggagaaaac gatccagacg ttgtattcgt tactctagga acaggtgttg gcggtggtat
    34501 cgttgctggc ggaaatttga ttcacggcgt gagcggagca ggcggagaga tcggccatat
    34561 cactgtggat cctgaagggt tcgaatgtac ttgcggtaaa cgcggatgtt tagaaactgt
    34621 gtctagcgct actggtgtag tacgtctagg tcgttacttg gcagaagaat acgcaggaga
    34681 ttcaaaactg aaagcaatgt tagacaacgg tgaagaagta acaagtaaag atatctttga
    34741 aatggctcaa gaagacgatc cgtttgcatt gatggttgtt gatcgcgtat gcttctactt
    34801 aggtttagct tgcgccaact tggggaatac attgaatccg tcaagtattg tgttaggtgg
    34861 aggagtatcg gcagcaggag aatttttacg tagccgtgta gagaaatatt ttaaagaatt
    34921 tactttcccg caagtaagag aatcaacaaa aatcaaatta gcagagcttg gtaatgaagc
    34981 tggtgtcatt ggtgctgcct cattagcctt gaaatttgct taaaaaaacg gaggcattac
    35041 atggttttat gggtaattaa tatcattttg ctattgattg tgcttgcaat catcttttgg
    35101 gaagtctatc tacgagtaat ggccaaacgt tcagcaacga ctttgacaga agaagaattt
    35161 cgagaaggaa tgagaaaagc acaagtaatc gatgttcgcg aaaaagatgt gtttgatgct
    35221 gggcatattt taggtgcaag aaacatccct tacactgttt tgaaagattc attaggatct
    35281 attcgaaaag accagcctgt ctatatttac gatcaaaaga aaagtttgag catccgtaca
    35341 gcaaataaat tacgtaaagc aggttatcaa gagatttatt tacttaaggg tggatatgac
    35401 ggatggtctg gaaaaatcaa aagtaaaaaa gtataaaaca gatgattgta tgacagcttt
    35461 aaaaaccgaa tcaatcatta gattttgacc aagatcacat gaacggcgca atggatagtt
    35521 gtgccgttca ttttttttat tttgtgctat tatctacata tataagctaa gcgaaaaggc
    35581 agaaagaagg aaacaactat gatcaaatta aaacgtgttt accttgatgc tgaaaaagag
    35641 gatggctata gaatactcgt ggataggttc tggccacgag gaatttcaaa agaaaaagcg
    35701 cagttagatc tgtggttgaa agaaattgca cctagtaatg aagcacgcaa aacttttgac
    35761 catgtaccag aacgatttgc ttcatttgca aagaaatacc agcaagaatt gattttcgaa
    35821 gaagaaaaga agcaagctat ttttaaactc agtcaactta tgaaagaaca tccagtcatt
    35881 acttttgttt atggtgcaaa aaatgaacat cagaaccatg ctgttttatt gaaaaaattt
    35941 atcgaaatgg aggcatgcaa atgaaaatag ggatcattgg tgccggacct agaggattaa
    36001 gtgtagcaga aaggattctc cataataatc aggacagaca ttctcttgaa ctttttcttt
    36061 tcgatcccaa tggtcctgga ggtcgagttt ggcggttgaa tcagccaccg gagttattaa
    36121 tgaattctgt ttctcaacaa gtgacgcttt ttacagatga aacattgtcc agtggaggat
    36181 tggtaacaca gggacccaac ttgtttcaat ggagtaaagg acctgctaca gattatatcg
    36241 aacagacggt aactaaaaat aagcaactaa ttatagaaga aatcaatcgt ctggaaaaag
    36301 atcagcaatc tactcgatgt ttatacggac tctatcaacg ctggtttttc tcaaagttgc
    36361 aagaagaatt tccagcttct attcagtttg tttattcttt agtaaaaaaa gtcagcaaaa
    36421 gaaaagatgg ttatctttta cagacagatc agcaaagcta ttctatcgat cagcttgttt
    36481 tagcaagcgg tcactgggaa aatgatgaga cagaagaaga aagagtgtgg gtagactacg
    36541 caaagaaaaa tgatttactg tatcagcttc cttctaatcc tgcagatgtc gatctttcga
    36601 agataccgcc taaagaaaat gtctttttaa gaggtctagg cttaagtttt tttgattatg
    36661 tcggattgtt tacagtatca agaggagggc gcttcgagaa cgaaaaggga aaacttgtct
    36721 atcatccatc aggcaaggaa ccgattgtat acagtggatc aagaaggggg ctcccttatt
    36781 accctcgagg aagaaaccaa aagcaaggcg gagcaatggc atggccacgg ttactaacaa
    36841 aagaaaacct ggagcaatgg catcagtcgg gatttttgac tggcgaaaca ttttttgact
    36901 atctgaaaaa agacgttgag ttgttttatt acaagaaaat catcgaagaa tatcatttgc
    36961 cgattccaaa gaaaaatttt gagcatgatt ttctctccct ctcttcagag gagtgtctcg
    37021 gaaagcatcc agcactaatc ccttatagat gggattggtc gtatttagaa attcctctta
    37081 cctatcagga tgagtccttt gcagaagcta gccgagcatt tattgaaggg cagataaaag
    37141 aagcggaaaa aggcaattgc acaggagcat ttgcttcaac atttgatgca ttaaaagact
    37201 ggcgtgatcc aatccggcag gctattgaat ggcacgtttt ttctgcaaag gaatacaaag
    37261 aaatactatg gggatggttt actcatctaa atagcttttt aacgatcggt ccgccagtca
    37321 ttcggacgaa agaattagca gcactgatag atgcgggtat ttttcatttg atcgaaccgc
    37381 cacttgatat tcagcaaaag cacaaccgct atgttatttc tctacaagat aaagaaatca
    37441 gcagtcgcta tttgattgaa gctcgattgc cacaaacaag cttttctcgt tcaagtaatc
    37501 ctgtcataaa aaatttgaag gaaaacaaac ttgttcgcct tttttcattt tcagataata
    37561 caggaagtta tcattcagaa gctatagatg ttgagcgagg tacgaatcgc gtgattgatg
    37621 cttcagggga aatccagtct gacttttatt gctatggaat acctgttgaa ggtgtggatt
    37681 ggctaactgc agcagcagct agaccttata cagatccatg gaatctgaga caggcagata
    37741 aaatagcaca acttatattg actgacaaat aaaaatacgc atgatgactt atctgttctt
    37801 tcaaaagtct tcatgcgtat ttttaaataa ttattaacga gaaacgcgtt tgcgcattgc
    37861 ttttttacgg ttttcttcga tcattgcttc tttttcgtca attggatcga tcactgtttt
    37921 tttcacagaa gcgaccaaac cagcaacggc agcggctgtt gccagacttc ctactaatat
    37981 tccagataca aattttttca ttgaatccac ctcatttatt atttgttata tccattatgg
    38041 aacaaaagag aaaaaaaggc tagttttacg tttgtttgta aacctttgca aaaaatcctt
    38101 gaaaatgttt actaaatgtt ttattataaa tttaccatat gaatttacga aattttactt
    38161 tgtttgtttt caccataaaa gtaaacttcg tttttttaaa ttatatataa tcaacaaaca
    38221 caattttact aatcaaagga gtgtttttta tgacaatttt agtactaggc ggagccggtt
    38281 atatcggttc gcacgctgta gaccagctag ttcaaaaggg gtatcaagta gctgtggtag
    38341 ataatttgct cacaggtcac aaacaagcag tacatccaga tgctcatttt tatgaaggag
    38401 acatccggga taaagaattt ctacgttccg tatttgaaaa ggaaccgatt gaaggagtaa
    38461 tccattttgc tgcaagctct ttagtaggag aatctgtcga aaaaccattg atgtatttca
    38521 acaacaacgt atacggtatg caaatcttac ttgaagtcat gcacgagttc aatgtgaata
    38581 aaatcgtgtt ctcttctact gcagcaactt acggagaacc aaaagagtct ccaatcacag
    38641 aagatacacc agctaatcct aaaaatccgt atggtgaaag taaattgatg atggaaaaaa
    38701 tgatgaagtg gtgtgaccaa gcatatggca tgcgctatgt cgctttgcgc tacttcaatg
    38761 tagccggagc aaaagcagat gcatctatcg gagaagatca tacaccagaa acgcatcttg
    38821 tcccaatcat tttacaagta gctcttggtc aaagaaaagc attggctgtt tatggagacg
    38881 attatgatac accagatgga acatgtatca gagattatgt tcaagtagaa gatttgattg
    38941 ctgcacatat tttagctttg gaatacttga aagaaggaaa tgaaagcaac ttcttcaatt
    39001 taggcagcag caaaggatac tcagtcaagg aaatgctaga agcagctaga gaagtgacag
    39061 gaaaagaaat tccagcagaa atagcaccaa gaagagctgg agatcctagc cgattagtcg
    39121 cttcaagtga aaaagcgaga gaggttctgg gctggaaacc tgaatacaca gatatcaaag
    39181 caattatcaa aacagcatgg gattggcatg tcagtcatcc aaatggttat gaagaatagg
    39241 ggaatgagat atgtcaatca gtcaaacgat ttgtgatttt gccacacttg cgatcgaagc
    39301 tggcggatgg atggaaatgg accgcttata tttacagaat cgtcttttag ggatggtagg
    39361 agaagatgct ttagaaagca ttgaagtaag accagttaag gaagaatctg tagacttatt
    39421 ggatcagctt gtcgctaagg caagagaaaa tggtgttctt tcagaattgc cagcagaaca
    39481 agagattttt gaagcgcaat taatggattt tctcacaccg ccaccttcag tcgtcaatgc
    39541 attctttgca cagcactatt cgaaaagccc agaggaagca actgagtatt tctttaattt
    39601 aagtaagaaa aatgactaca tcaaaacaag agccatcgca aaaaacatca aatttccggc
    39661 agagtcggag tatggtgaac tggaaatcac aatcaattta tctaagccag aaaaagatcc
    39721 aaaacaaatc gcagcagaac gcgaggcggt aaaagtagac tatcctacct gcatgctttg
    39781 catggaaaac gaaggatata gaggtcgctt gaactatcca gcccgcacca atcaccggat
    39841 tattcgaatg aatctagacg gtgaaagctg gggattccaa tattctcctt atgcttatta
    39901 caatgaacat tcgatcattt tatcagaaca acaccgcccg atgaaaatca caaaggaaac
    39961 atttgctaga ctgcttcgga ttactgaagt cttgcctcat tactttgttg gttcgaatgc
    40021 tgacttgccg attgttggag gatcgattct ttctcatgat cattatcagg caggacgtca
    40081 tgaatttcct atggcaaaag caccgataga aaaacttatc aaactagcag attatccgca
    40141 agtcatcgca ggagtcgtga aatggccgat gtctgtgatc cgtttgcaat caacagagaa
    40201 aaacttgctg attgaagcag ctaactcagt attggaaaag tggaaagcat attcggatga
    40261 aacagtatct atcagagctt tttcagcaga tggtacacca caccatacga ttacgccaat
    40321 tgcacgaaga agggacaatt actttgagtt ggaccttgtc cttcgagaca acaatgtttc
    40381 agaagaacat cctgatggga tttttcatcc acataaagat gtccaacata tcaaaaagga
    40441 gaacattggg ttgatcgaag taatggggct tgctgtttta ccacctcgtt tgaaaccaga
    40501 actagtagaa gtagagcatt tcttattagg agaagaaaac cagatggctg aatatcatcg
    40561 tccgtgggca gaacaattaa aaaaagaaca tcctgatttg acgaaagata cggtcaccga
    40621 tgtcgtacaa aaatctgtag gtcaggtttt tgcacgtgta ctggaagacg ctggagtatt
    40681 caagcgagat gaaaaaggac aggcagcgtt tgaacgattt actgcaattt tataaacgta
    40741 aaaaaggagg agagacaaat ggcaacaatt aaagacattg cgcagttggc aggagtttct
    40801 cctgctactg tttcaagagt tctcaactat gatcctgatc tttccgttgg cttagccaca
    40861 aagcaaaaaa tttttgaagc agcagaacgc ttaaattata cgaaacatcg taaaaatgca
    40921 gcaaatacta agcaaacgct tcgcctgatc cagtggtacg acgatacgga agagcttgaa
    40981 gatctttatt atttatctat tcgtctagga atcgaaaaaa aggcagaaga agcaaacgtt
    41041 actttattga aagaaacgtg ggattccatt agtgatcttc cgacagatgg aacgatcgca
    41101 ctaggaaaat tcgacgaagc tcagttatta gaaatgaaag gatcccaaaa tgccttgctt
    41161 ttggtagatt ctgatggcac ttctcaagga atcgactctc tagttgtcga ctttaagtca
    41221 agtgttcaaa aagtcattga acattttgtc tcccataaag cgcaacatat cgctatgctt
    41281 gctggaacag aatatacgaa gaaaaaccat cagcggctaa aagatcccag aattatggca
    41341 tttcaacaag ttttggcaga aaaaggctac gacgaaggtc caattatcga agcagatttt
    41401 tctgtcgagt caggatatca agcagtgaag cagtatttaa aaacgaatcc gcatgttcct
    41461 gatgcgcttt ttgcagctaa tgatgcatta gcgattggcg cactgaaagc gattcaagaa
    41521 aaaggtttag tggtaccaga agatatttct gtgatgggtt tcaatgatgt cagtgtagcg
    41581 aaatatgtaa cgccctctct ttctactgta aaagtttata ccgaatggat gggagagcta
    41641 gcagtggaga cgattcttca attaagtaat tccaaagcac cggtttcaag aaagattatt
    41701 gtggaaacag agttgattat tcgagaatca acgaaataaa agatggaaaa gctgtgggga
    41761 aatggagtga taccgcagct tttttgtgtt tcaggaaggg gcaaaatttg cccgccagca
    41821 gataatcatg tatcattgta ctagttttgc ctttggtatt ttcatcctca gaataaaaaa
    41881 gtatgctaca atggaaggcg agaatgtcat agcagaaagg atcaaatata tgattacatt
    41941 aggaatagat actgcaaacc aaactttggc gatcggtgtg gtagaagacg aacaaattct
    42001 tggacaaata caaacaaata tcaaacgcaa ccacagtgta acactgatgc ctgcaatcga
    42061 ccaattgttt gcagatcttc aaataagtcc aaaagacatt gaccgaatcg cggtttctga
    42121 tggtcccggt tcttatacag ggcttcgtat aggtgtaacg acagcaaaaa cgattgctta
    42181 tacattagat aaagagctag taggagtatc tagtctgaaa accatcgcgg ctaattgcat
    42241 cggtgtggat aatattattg tgcccatgtt tgatgctcga agaaaaaatg tatacgctgg
    42301 ggcataccgc tggaaagaaa atagattaga aactgtactt gaagatgtac atatttctgc
    42361 ttctgatttg ttcacacaac tacaaaagct gaatgaagct atttatttcg tcgggagtga
    42421 ttgtcacaaa ttcttctctg aaataaagga aatattgcct agtgcgatga ttaatacagt
    42481 tccagcatgg gatattccaa gtggtgtaac tgtggctcaa ttaggtgctt tagctgaacc
    42541 agttgcagag aaacaagctt ttcttcctcg ctatttaaaa cgggtagaag cagaagaaaa
    42601 atggttggag acgcacacac ctggagacga aagttatgtt gaaaaaattt agagcgtatg
    42661 tcagaagctt ttttcaagat aaaatgattt atacagaaaa aacaatcgaa caaatcgatc
    42721 gtcgttacgt actaagagaa ttgactacag aagatatcaa agagctgctg gatgtcgaac
    42781 gagaagtcta taatggcgaa ttgccatgga caaaaagtgc ctttatatca gaattgaagt
    42841 caccgcttcc tcatctatat ctaggtatct ttgataatgg aagaatgatt ggttttatcg
    42901 gttcaagagc gttcggttta gattgccata tcacgaatat tgccgttcga ccggcatatc
    42961 agcgtaaacg aatcggttcg ctgcttattg acgaaatcga gaactttgct ataatgaatc
    43021 gctgtgagac gatgtcatta gaagtgcgta tgagtaatca agatgcacaa agactttatc
    43081 ggaagttagg ttttcaagca acgacgatca aaaaaagata ttacactgaa acaaaagaag
    43141 atgcattgga tatgataaaa cttttagaaa atgagtgaga ttagttgatt tgcagaaaaa
    43201 aagattttga tgaagcgtac ttggcagaat gcgtatgggt gattagcgaa aatgcttatc
    43261 cacatggttc accttggacg aaaatgcagt ttttggcaga catccaacag cctcactctg
    43321 attatctgct tttagttgaa gataaccaga tctgtggctt tattggatac tcgaaagtaa
    43381 tagatgaagt cgagatcaca aatattgctg ttgcagtttc agaacaagga aaagggcatg
    43441 cacggcattt gttacaatta ctgattacgg aacaaataaa agattcgctg catgtttttt
    43501 tagaagtacg tctatctaat gaagcagcaa gaaaacttta tgaatcagaa aagtttcgta
    43561 tattaggtaa acggaaatca tattatcgca atccgacgga agatgcgatc attatgagca
    43621 gaaagttaaa aacggagacg atgaaatgaa tgaagaattg attctggcga ttgaatcaag
    43681 ttgtgatgaa acgagtgtag cggtcgttag aaatggaaca gagatcttat ctaacatcgt
    43741 tgcttctcaa ataaacagtc ataaacgttt tggtggcgta gttcctgaag tagctagccg
    43801 ccatcacgta gaacaaatca ccttatgctt ggaagatgca ctggtagaag ctggtgtgtc
    43861 ggcagaagat ttatcggcag tcgctgtcac ttatggacca ggacttgtcg gctcgttgct
    43921 gattggtatt tctgcagcta aggcttttgc ttgggcacac caattgccgc ttattcctgt
    43981 aaatcatatg gcagggcata tctatgcagc ccgtctggtt aagccattcc agtttccatt
    44041 gatggcactt cttgttagcg gaggacatac agaattagtc tatatgcaag aagatggatc
    44101 atatgaaatc attggggaga caagagatga cgcagcagga gaagcttatg acaaagttgg
    44161 aagagtatta ggtctttctt acccaagcgg aaaagaaatc gatcagctag ctcatcaggg
    44221 aaaagataat taccattttc cacgagcgat gatccatgaa gataattatg atttcagttt
    44281 tagcggatta aaaagtgcat ttatcaattt ggtgcataat gcacaacagc gtggagaaga
    44341 tctagacaaa aatgatttag cagctagttt tcaagcaagt gtgatcgatg tattgattac
    44401 taaaacactt cgagcttgtc aaaattatcc agtcaagcaa ctgattgtag ctggaggtgt
    44461 agcggccaat caagggttgc gtaaaggatt gcaggatgct ttggcaacta aactccctga
    44521 ggtagaacta gtgattcccc ctcttcgact ttgtggagat aatgctgcaa tgattggagc
    44581 agcagcacat gtggaaatgc aaaaaagaaa ttttgctagc tatcagttga atgctgatcc
    44641 aagcttggtt ttatctgaat aaagaggttg tgaaaaatct gccctgcatc aagtaataag
    44701 aacagcccag gaaaaacggc ttctcatgtt tttgacctgg ctgttcttgt taccgcgtat
    44761 gcaagctttt gaacgccgtt taatcaagag ttcgtaagcc tgtcgtttaa ctctgattca
    44821 caaggaacaa tttcaaaaaa cagctcctca tgtttatgaa cattatgact tgtgaccgaa
    44881 gagttgacag gtacacaccg tttaatcgaa ataagggctg tgacacgctt ttgtcaaagc
    44941 cctatttttt acagcttatt catcattatc cgttcctctg attgccctca ctgattaatc
    45001 gtttattgtc gaggaatatc gaataatcta ggtcggatga ggacaagacg cttcctctag
    45061 ttaatctatc agtatttttt gcaaacagtg aatattacat aactgaaaca cacatagaaa
    45121 atctttgagt cttgcccagt gatctaaaac tgccggatct ttcagaagtt ttagggagga
    45181 aattaagcag tgaccctgat aaatatcagg gtcactgctt atttttgttc gcgttattct
    45241 ctcatagctg gagtagtagc tgtcttacag aaagtaaaac gctttttgtc ataatctaac
    45301 agtccggaat cacgtagttg tttgattact tgtccagcag tttcacgtgt cgtgccgcta
    45361 ttcactgcta attcttttaa ggtgatcgga taaggaatgg tgatatgacc atcttttgtt
    45421 ggtacgccca attcttcttt caaaatgaat aaattttgtt ttacccggtc aaaggcgcta
    45481 gaggtcaatc cgatttggat acgcaattca tgtatttcaa ctactttaga aattttttgt
    45541 atcatataga tcaactgttc gggatttttt cgtgctaatt tttcatagta ggaaacagga
    45601 aggtagaatg tttcaatatc agtcatcgct tgagctgtat aagtgtaggt ctcttctcca
    45661 aacattcctg acataggaaa aaggttattt gtattcacat agtcatagta gtaataagtt
    45721 gcactttcat cataacgttc taaacgaacg agtcctttgg tcaagatata caaccgttgt
    45781 ctgggatcac catcgtcgaa aaggatctgt ccttttttat aagaacgaag aacagtatgt
    45841 tccttgatct gttggaactc ttcatccgag aaatgttcaa aatcaggttg atatctgagt
    45901 tttgaaaagt gaaaatcgat tttattaaca cgcattaaaa agtcctctcc ttattgttca
    45961 ctatatttat tgtcactttt tttaaagaat ttgtaaagtt tta
//
LOCUS       DAGCXG010000015        43517 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGCXG010000015 DAGCXG010000000
VERSION     DAGCXG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.015"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(18..710)
                     /locus_tag="MB022_000820"
     CDS             complement(18..710)
                     /locus_tag="MB022_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4465839.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="MB022_000821"
     CDS             705..971
                     /locus_tag="MB022_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4465840.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="MB022_000822"
     CDS             complement(1147..1716)
                     /locus_tag="MB022_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4465841.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..2113)
                     /locus_tag="MB022_000823"
     CDS             complement(1790..2113)
                     /locus_tag="MB022_000823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465842.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2267..3079)
                     /locus_tag="MB022_000824"
     CDS             complement(2267..3079)
                     /locus_tag="MB022_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465843.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIREKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3433..4281
                     /locus_tag="MB022_000825"
     CDS             3433..4281
                     /locus_tag="MB022_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBT4465844.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRIHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4295..4618
                     /locus_tag="MB022_000826"
     CDS             4295..4618
                     /locus_tag="MB022_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465845.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLLLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5366
                     /locus_tag="MB022_000827"
                     /pseudo
     CDS             4615..5366
                     /locus_tag="MB022_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            5356..7527
                     /locus_tag="MB022_000828"
                     /pseudo
     CDS             5356..7527
                     /locus_tag="MB022_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7639..9810)
                     /gene="copB"
                     /locus_tag="MB022_000829"
     CDS             complement(7639..9810)
                     /gene="copB"
                     /locus_tag="MB022_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBT4465846.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9830..12016)
                     /locus_tag="MB022_000830"
     CDS             complement(9830..12016)
                     /locus_tag="MB022_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBT4465847.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="MB022_000831"
     CDS             complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="MB022_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBT4465848.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12238..12678)
                     /locus_tag="MB022_000832"
     CDS             complement(12238..12678)
                     /locus_tag="MB022_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBT4465849.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12752..13291)
                     /locus_tag="MB022_000833"
     CDS             complement(12752..13291)
                     /locus_tag="MB022_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4465850.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13906..15372)
                     /locus_tag="MB022_000834"
     CDS             complement(13906..15372)
                     /locus_tag="MB022_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4465851.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15683..17764)
                     /gene="cadA"
                     /locus_tag="MB022_000835"
     CDS             complement(15683..17764)
                     /gene="cadA"
                     /locus_tag="MB022_000835"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4465852.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(18289..18648)
                     /locus_tag="MB022_000836"
     CDS             complement(18289..18648)
                     /locus_tag="MB022_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBT4465853.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18678..19019)
                     /locus_tag="MB022_000837"
     CDS             complement(18678..19019)
                     /locus_tag="MB022_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465854.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(19016..19690)
                     /locus_tag="MB022_000838"
     CDS             complement(19016..19690)
                     /locus_tag="MB022_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4465855.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20866..22164)
                     /gene="asnS"
                     /locus_tag="MB022_000839"
     CDS             complement(20866..22164)
                     /gene="asnS"
                     /locus_tag="MB022_000839"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBT4465856.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22204..23394)
                     /locus_tag="MB022_000840"
     CDS             complement(22204..23394)
                     /locus_tag="MB022_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4465857.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PKNIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTFFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23415..23942)
                     /locus_tag="MB022_000841"
     CDS             complement(23415..23942)
                     /locus_tag="MB022_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBT4465858.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23989..26778)
                     /locus_tag="MB022_000842"
     CDS             complement(23989..26778)
                     /locus_tag="MB022_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4465859.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLFAATIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEEQMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKTAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIQEFLKKNEEGS"
     gene            complement(26925..27125)
                     /locus_tag="MB022_000843"
     CDS             complement(26925..27125)
                     /locus_tag="MB022_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4465860.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27577..27897)
                     /locus_tag="MB022_000844"
     CDS             complement(27577..27897)
                     /locus_tag="MB022_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465861.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(28400..29518)
                     /locus_tag="MB022_000845"
     CDS             complement(28400..29518)
                     /locus_tag="MB022_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465862.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            29989..31296
                     /locus_tag="MB022_000846"
     CDS             29989..31296
                     /locus_tag="MB022_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBT4465863.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            31415..32614
                     /locus_tag="MB022_000847"
     CDS             31415..32614
                     /locus_tag="MB022_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBT4465864.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(32640..33908)
                     /locus_tag="MB022_000848"
     CDS             complement(32640..33908)
                     /locus_tag="MB022_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBT4465865.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPGWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(34071..34718)
                     /locus_tag="MB022_000849"
     CDS             complement(34071..34718)
                     /locus_tag="MB022_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
                     /protein_id="HBT4465866.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSYVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(35126..35629)
                     /locus_tag="MB022_000850"
     CDS             complement(35126..35629)
                     /locus_tag="MB022_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4465867.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(35752..36516)
                     /locus_tag="MB022_000851"
     CDS             complement(35752..36516)
                     /locus_tag="MB022_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBT4465868.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            36623..36898
                     /locus_tag="MB022_000852"
     CDS             36623..36898
                     /locus_tag="MB022_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBT4465869.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            37336..38286
                     /gene="yidC"
                     /locus_tag="MB022_000853"
     CDS             37336..38286
                     /gene="yidC"
                     /locus_tag="MB022_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4465870.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVDNFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(38465..39439)
                     /gene="lacD"
                     /locus_tag="MB022_000854"
                     /pseudo
     CDS             complement(38465..39439)
                     /gene="lacD"
                     /locus_tag="MB022_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            complement(39436..39899)
                     /locus_tag="MB022_000855"
                     /pseudo
     CDS             complement(39436..39899)
                     /locus_tag="MB022_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(39896..41359)
                     /locus_tag="MB022_000856"
     CDS             complement(39896..41359)
                     /locus_tag="MB022_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4465871.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKVVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVSM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVLAPHGGFLVLPVVTGKIQWVTAIVIGSIVGG
                     VILGLIQKKRVTDKDKKMDSKAANRTVIEKGRAGDLS"
     gene            complement(41341..42291)
                     /gene="pfkB"
                     /locus_tag="MB022_000857"
     CDS             complement(41341..42291)
                     /gene="pfkB"
                     /locus_tag="MB022_000857"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4465872.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQAAVHNFLNQIRVLQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIREERFERWENIN"
     gene            42434..43183
                     /locus_tag="MB022_000858"
     CDS             42434..43183
                     /locus_tag="MB022_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4465873.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQKIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT        12535 a         9223 c         7638 g        14121 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttc
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctctcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat tcttggtaaa
     2281 gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 ctcttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag tgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaattcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacttct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt
     4861 ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt
     4921 ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc
     4981 ctcttttttg cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt
     5041 tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg
     5101 cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga
     5161 aagctttttt cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg
     5221 agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga acggtttatc
     5281 ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc
     5341 caggaggtga atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt
     5401 gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac
     5461 cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg
     5521 gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg
     5581 aagacatttt ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc
     5641 catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg
     5701 ttgctgatct atttatttat tacattgacc gtggattacg actattggta cgcacccttt
     5761 ctttctttga tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac
     5821 gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc
     5881 atccggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct gcttatttgt
     5941 ttcttaactt ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag
     6001 gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat
     6061 gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt
     6121 ggacctttat atgataatgg cgaagttgtt tttacagcga accaagaagg aaatggctat
     6181 tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga tcaggacgca
     6241 cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa
     6301 gacaaagaaa acattacatt gaccctaaaa aaacaagttt tcttacctta tccttatgga
     6361 aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa
     6421 agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa
     6481 aggttagttt ttacgagaga agaattaaaa atcagtcagt taaaagctga tgatttagaa
     6541 aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg
     6601 gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat
     6661 ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccgggcatac tccaaaagaa
     6721 cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca
     6781 acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt
     6841 acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat
     6901 agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa
     6961 ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc
     7021 gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt
     7081 gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc
     7141 ttattcagcc ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa
     7201 tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt
     7261 gcatatcaaa aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct
     7321 ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga
     7381 ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt aaaattcgat
     7441 aaaaaaatct atttagaaat aacgcatata ataagaaaaa tggtatacaa aaaaattctg
     7501 acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctggaatg
     7561 ccgagaacca gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt
     7621 cacaaccttt tttcattatt atctgatttt caatgtcaaa gcatttagag ctacaactac
     7681 cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac caattggcgc
     7741 aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc accagaggtt
     7801 ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg
     7861 atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc ctgcaccgat
     7921 tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat cacccaccat
     7981 gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat catctggtaa
     8041 taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg cagcgttttt
     8101 attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc ggataaattc
     8161 ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat gattttctaa
     8221 aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg cttgttctac
     8281 gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt gttttttgac
     8341 tgttgcctct agcccgactc ctgaaagatt ttttaggttt tcagcttgat aaggcttgat
     8401 ttcatgttct ttcaaataat tcataatgcc aacagccaat ggatgattgg cattcgcttc
     8461 caaggctcct agatattgaa ggatttcatt tttattgaac gcatcatcaa gtacttcgac
     8521 acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca tgatcacatc
     8581 tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc
     8641 tattgatgtg gaacgagcaa caactaatgg gatagctaaa cctagagcat gaggacaggc
     8701 gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca agaacaacca
     8761 ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt ttgccacttt
     8821 gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca tttccataac
     8881 cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg ttccatcccc
     8941 attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc
     9001 agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat ctgttggcat
     9061 tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca atgaaactgt
     9121 ctcttctttt ccatctttcg tcaagcgttt cactgattct ggcagtaatt ctgctagttt
     9181 ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac ctagtagcat
     9241 aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat gtggattgag
     9301 cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc ccatcgcgat
     9361 cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac ttaaaaatgg
     9421 ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat cagaaccagg
     9481 aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa tgattgggat
     9541 agctaggata agcgatagcc aaaatttttg tttgaaattc cccatgtgca tggaatgatc
     9601 catgccgccg tgttcgtgat ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat
     9661 atctccatgg ttatgtccca tgtgcatgga atgatccatc tgactatggt catgtttcat
     9721 ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt ttttatgctc
     9781 atgttttttc tccatatcgt tcccattcat ttttactcca tcccttctct tactttttca
     9841 tgtgtcgatt taagctcagg gagttcaaca atacactgac ggaactaaac gccatcgctc
     9901 ctcctgcaat gattggattc aaaaagccta gtgcggcaaa aggaatacca atcgtattat
     9961 agacaaaagc ccagaaaaga ttttgtttga tttttctcaa tgtcaaccta gacaactcga
    10021 ttgtttgtgc aatggatgcc aaactgctat tcatcagcgt cacatcggct gtttccatcg
    10081 caatgtccgt tccacttccc attgccatac cgacatcagc taatgctaag gccggtgcat
    10141 cattgattcc atcgcctgcc ataccgactt ttttgccatc tttctgaagt ttttctacgt
    10201 aggctgcttt atcctctgga agtacttcag caaagatatg ctttggatca ataccaactt
    10261 gtttaccaat cgtttcggct gccagtttgt tgtctcctgt cagcataaaa acatctaatc
    10321 ctttattctg gagctgcttg attgcttgtt tgacttctag tttgatttga tcagccacgg
    10381 caatcaaccc gatcacttgc ttttcatcag ctaaaaacat gactgttttt ccctgctgtt
    10441 ctaattctaa cgcgtgttct tggtattcat caaatgatag gttcaattca attaatcgtt
    10501 tacgtgtacc cgcaaaatag cgtactccgt taatcgtacc gctgattcca gcacctgggt
    10561 gggctgtaaa atcaatcata tcgtatgttg ctgcttcttg cagttttccg tattccacga
    10621 ttgctttacc tagtggatgt tcagaagctt gttctaaagt ataaaaaata gataggactt
    10681 ctttagaacc gactagatct gtaactttgg gctttccttc tgtaatcgtt ccagttttat
    10741 ccaaaacaat gctgtctaag tgagcagctg cttctaatgc ttctccgcct ttaattaaaa
    10801 ttccatttcg tgcaccaaca cccgttccta ccataatagc tgttggagtc gctaaaccta
    10861 atgcacatgg acacgcaatg actaatacag agacactatg gatcaatgcg agttgccaat
    10921 caccagtgat caaacctgta gctatgagcg tgataaacgc gatgactaat acgatcggaa
    10981 caaaaatccc tgagatccga tctgcgattt tttggattgg ggctttgctc ccttgagcat
    11041 cttccaccat ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt gtaacttcca
    11101 tgtgaagtag accattcgta ttgattgtcc cgccaaagag cgtgtcatcc ggattctttt
    11161 ctacaggaag attttcacca gtaagcatac tttcatctat cgcactgctt cctgaaataa
    11221 ttcgaccatc tgctggaatc tgctcaccag gacggatgac tagttgatca ccaacgacta
    11281 cttcttcaat cgctaacgtt tcttctttcc cgtttcttat cacttgggcg gtttttgttt
    11341 gaagagacat caattgtttg attgcattac ctgttttcgt ttttgctgta tgttccaaat
    11401 atttgccgag caaaatcagc gtgatgatca tactgctgct ttcaaaataa agatcttggg
    11461 ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg
    11521 ctaccaatac atccatattt ggtgctttcg tcttgattgc atggtacgca cctttataaa
    11581 aacgagcacc gacataaaat tggacaggag cagacagcac taactggacg attggaaaat
    11641 gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat agcaatcatt gacaacatca
    11701 acggcaaagt caaaaccgta ctcaatataa gatcgaatag catttttttc aaatacgctt
    11761 gcttttcttc tgcaattttt tgtttgtggg cttcatcgta caaaattgca ccatagccga
    11821 tgttttctac gctttggatt aatttttcag tggtcgtccc ttcatatttg acacttgctt
    11881 tttcagtggc caagttgact gtggcattga ccacaccagg ttgtccgttc agctcttttt
    11941 cgatcctagc agaacaattt gcacaagtca tacctgtaat gacgaacgta tccattttgg
    12001 tttctgctgt tgccatcaaa tcacctctgc tttataaccc aatccattga ttgcttgaca
    12061 aatcttttcg gattgaactt ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa
    12121 atttactttc actttttgca cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc
    12181 acaatgattg cagctcatac cttcgattga aaattgttgt ttcatttttt cttcctccta
    12241 ttgatgttgt ttacattcac attgacctgg gatacagtta cattcgatcg tatctactgg
    12301 ttgcttttgt tccagttgtt tcaaaatctc attgatatct tcttgagtca aagtggcttc
    12361 ttctactaga tccgcaatcg tctctccaat cctttttgca cagatatggg aaaaaagatt
    12421 ttccgtcgca ctgcgaactg tttccatctc cgagacagct ggatgataaa tgaatttttt
    12481 tccttcttgt tctgtccaga tcacttcttt ttttaccaaa cgacccagca aagttttgat
    12541 cgttgctggt ttccaatcca tcgaatcagc taagatctgc gtgatttctt gggctgtcgt
    12601 ctgtccaagt gtccaaataa cacgcatgat ctcccactct gaatcactga tctttactgg
    12661 cgttttgact ttttccattt atttcacacc tttcgtttac aaatgtaatc attatcttat
    12721 gaatacagat tacacttgta aacaaaaact gtcaattatt tttgctctct attttcaatt
    12781 tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat gatcagttta tttcttgcat
    12841 tagctacttg gatcagctgc tgtattttcg gttcattgtt ttcttcaaaa tgccagataa
    12901 aagataaaaa ttctaagtat tccctgttta gtttctccga aaatgattca ggcatatctg
    12961 gccgagattt tttgtttatc tttcttttta gagaacgcca aactactcgg tagatggcga
    13021 gataacgcgg cactttaagc caaatgatct tatctgcttc tttaatcctt tcctctaatg
    13081 tagaagtata atttccttca acgatccatg acggattaga taccataaac aattgctgtt
    13141 cttgcaaaaa ccactttttt gcctgaatac tgtagtctgt tgtatgccat aaaaggtcta
    13201 gcggcagtag cggaaagtcg gtgatttctt tgatcttcct tgcgagggtc gattttcccg
    13261 acccactttg tccaatcacg atgatcttca ttcttctccc tcacttctct ttggaatttg
    13321 gttcgatcat accacttata acgccgcaaa aaaacaaggg actgtgacaa aagtcgtgtc
    13381 aaagtccctt gtttcgaata aatggtgttc aatcagctga ccttcagcat tagctcaaaa
    13441 agcggcaaaa tatgaggagc tatttttccg tttgtttctc taatactcag gtcaaatcgc
    13501 tgattttaca acctcttcct taaacggtgt tctcctgcca acacctcggt cacaagtcac
    13561 aatagtcaac aaacatgaga agttgttttt tgaaattgct ccttgtgact cggagttaaa
    13621 cggcaggctc actacctctt tcttaaacgg tgttccaaag cttgcatctg cggtaataag
    13681 cccagccaaa cgaaaaatat gaggggctat ttttgtttgg ctgttcttat tacttgatgc
    13741 aggacagctt tttcacaacc tctgattcac ggtgttccag aagtagcttc ttcgaaaaac
    13801 aagccgaaac ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    13861 agctgaccac ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac
    13921 tgtttctggt tcagtgattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat
    13981 acctaataag atcaagacga ttggtgtgat gatattcgta aataattgga accaccatgc
    14041 tttaggatca gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt
    14101 gaaggcaaag caccaagcac caaagataaa accgattttt ggatttttaa caaatttata
    14161 ttcagaattg aatttcttat aagctttatt caacatcata tatgccaaga acacccatag
    14221 gtaacgcatt ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat
    14281 atctttgata ccaaaaatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag
    14341 ataaccattc accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc
    14401 tggatccgca tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga
    14461 gatttgtcca atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata
    14521 gtaattacct aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttaggaat
    14581 attatcacta gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac
    14641 cattgctgct aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt
    14701 gacataaggg gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt
    14761 gaaataactg aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc
    14821 aaattccatc gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga
    14881 catgacgaac atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt
    14941 tgttgataaa tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac
    15001 tgacatcgta tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat
    15061 tgcttgaggc ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata
    15121 gtatgccagt cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact
    15181 atccttgaag gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc
    15241 caaaatcaat agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac
    15301 attccccatg ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg
    15361 tttgttgttc attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat
    15421 tcaattttta acaaacttac aagacagtgt tattttatgt gtgtattctt ttatttaata
    15481 cacatcgttt tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg
    15541 accttattga atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt
    15601 ataatcctat ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac
    15661 gttcattcgt cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat
    15721 tcaaaacagc taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa
    15781 aaccaaaagc acctaaggca agtaccaaaa tttttatccc aatagcaaag acaatgtttt
    15841 gtttgacgat tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt
    15901 gatcattcat gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca
    15961 ttgcgattcc tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa
    16021 atgcggtctt tttattgttt ctttgtaaga tttcctctaa ccgctgcact ttatcttcag
    16081 gaagcaattc gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt
    16141 ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc agcctgtttt aattgaacca
    16201 atgcgtctac ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt
    16261 gatcgatcgc tagataaagc aatgtaccga tttcttgact taaaggaaaa gagatttcaa
    16321 atctttccat caatttgtga ttacctgcta atacttgttt gccatcaact tcagctaaga
    16381 tcccttcacc tgcaaattcc tggatattag ttgttggtaa aagcggacct gcgtagcctt
    16441 ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca atatattgca
    16501 tgaatttctt tggatcgatt tttgtatcga tcgtctggat cgaaaagtca cctttcgtta
    16561 atgtgccggt tttatcgaat accactgtct cggtttgcgc aagcaactcc aagtaatttg
    16621 aaccttttac taatacaccg attttacttg cgcctccgat accgccaaag aaactaagtg
    16681 gcacggaaat cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga
    16741 tccattcaga aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg
    16801 ccaatcctac aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg
    16861 gtgctttttt actgctggca ttttctacta attctaataa tttgctgata gtagaatttt
    16921 cataagattt ttctacttga actaaaaatg gtttattttt attgataaat ccgcttaata
    16981 ctgtttcacc aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga
    17041 ctaatgattc tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa
    17101 gtacaaactg accgatctgc acgtcttcag ggttcacggt catcaatcct tccgtcgtct
    17161 gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac
    17221 tgactgccaa tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt
    17281 ctggatattc accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat
    17341 caaagatttt tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca
    17401 gcagataaat caataaatac cctatcaagc gaattggttc attaaaatca aataatagca
    17461 ataaagcgat acctgcaagg gaaaataaaa tccggaacag cgtatttctc tctttttcat
    17521 ttccatgttc agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt
    17581 ccttcactac tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc tctgcgtcaa
    17641 tcgttagttt tccagaagtg aagtttaccg ttgcttcctt gactccattg atcttctgaa
    17701 ctcctttttc gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt
    17761 tcattttaat gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg
    17821 aatggttatt catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct tcatcggaaa
    17881 atttcttcca tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgcccctg
    17941 ctcttatcca acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc
    18001 ctgttgctgg caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa
    18061 tccctgcaaa atgtccttcc cagatattcg aagctccact acgctttcaa aaatatagaa
    18121 agagttcttt aatgaaaatt atttcaactt tccataaaca gtaatcccat ataaataaaa
    18181 gacgtaaaag tcacatggga gaacttatac aggaaaacac atagagtcag gtgaaataca
    18241 tgtatcccac ctgactctat gcgttttcta acctttatta ttctcttttt atacaatttc
    18301 tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat
    18361 ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag
    18421 gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact ggtgataaat
    18481 tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat
    18541 tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc
    18601 gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct
    18661 tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct ttttttagct
    18721 tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc ttttgttact
    18781 aatgtgtgtt ggattagttt tcctgatggt cgctgatcca cccgttcttc tgactgttgc
    18841 acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt
    18901 ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca
    18961 gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccgtat ttccatcatt
    19021 tctcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg gcaattgcat
    19081 ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc
    19141 ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatgcca tctaaaggaa
    19201 ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt
    19261 ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga
    19321 tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt
    19381 gctgcgcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat
    19441 tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc
    19501 gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaattcca
    19561 tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat
    19621 cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct gtcaccgata aataatatac
    19681 gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta
    19741 tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta
    19801 gtcccttcaa tcaattgaat gaacgctcat caagtgtcta gttttttata ctttttttct
    19861 taacgtgccg gattcccctt gattccttcg ttgtttggtc cagagaaatc gatcacacct
    19921 aagcttccgt caatcagacc actgatgacg ccatcagaac atatatttat aggctcatct
    19981 aagacttcca gtaaatgttc ttttactaat atctcttcta gattgcttgg gatagattgg
    20041 tcataacttt atctggtccg atgatttcaa ggatgaatta aacttacata tggatcattg
    20101 catgctgaaa atacggacct actccatctt cgaatgttac tattagtttg ttttcaggta
    20161 ctagatgctg ctttatttca acagcagaag catcatcaaa tcagatttcc atttgaataa
    20221 ccttctttgt atatgtttga ttgattgcga tttcgttata gaaccttgtg aatcaaatag
    20281 caatttcttt tgctttcttc tcttgtctgc tggaaactta ctttatcccg tattttaaaa
    20341 aaagaactgc gagagttcgc agttcttttt tttaaataaa cgttgctctc cggccaactc
    20401 ctcggtcaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt
    20461 gtgactcgga gctgaacggc aggcgcacaa cctcttgata taaacggtgt tcaatcagct
    20521 gaccctcgat attagctaaa aaaacggtaa aatatgagaa gctatttttc cgttttgttt
    20581 ctctaatact caggtcaagg cactgatttc acaacctctt ccttaaacgg tgttcaaaag
    20641 ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
    20701 ttttcttaat actcgaaact aaacagcttt ttcacaacct ctccactaaa cggtattcaa
    20761 aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa catgaacagc tgtttttgct
    20821 tggctgttct aattacttga tgcagaacag ctttttcatc acctcttatg gataaattcg
    20881 gttcaacaaa cgtgggaatg gaatagcttc acgcacatgg tcgataccag caatccaagt
    20941 gactgttcgt tcaagaccta gaccaaaacc agagtgcgga acagaaccgt atttacgtag
    21001 atcaagatac catgaatatt ctttttcatc cagaccgtga ttacggattt gttctagcaa
    21061 gaaatcatag tctgttgccc gttcgcttcc gccaataatt tcaccgtaac cttctggtgc
    21121 gatcatatct gcacagatca ccacgtcttc tcttgttgga tgtggtttca tgtagaatgg
    21181 cttgatggct tttggatagt ttaggataaa gacaggctgt tcgaatgaat tcgcaatgaa
    21241 cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg tcatcaaatc catttttctt
    21301 cagtaattcg atcgcatcat cataagtaat acgagggaat ggcaattttg tataagtttt
    21361 taagacttct ttgtctcgtc ctagtacatc taatgcgtat tcgcaatgat ctaaaacatt
    21421 ttgtactaag aatgctacat attgttcctg tacttctaaa ctttcttctt gatgagtgaa
    21481 agccatttct ggttcgatca tccagaattc aatcaaatga cgtcgtgttt ttgatttttc
    21541 agcacggaaa gtcggaccga atgaaaagac ttttccgaaa gccatagcag cagcttccat
    21601 gtataattgg ccactttgag ataaataagc tttttgatca aagtaatttg tttcgaatag
    21661 atccgtagtg ccttctggtg cggaaccagt caagatcggc ggatcgattt tgacaaaatg
    21721 atgttgatta aagaattcat acgttgcacg aatgatttca tttctgatct gcataatcgc
    21781 atgttgtctt gaagaacgta gccataaatg acgatgatcc atcaagaagt ctgttccgtg
    21841 ttcttttggt gtgatcggat aatcgtggct ctcgcttacc acttccacat ttgtaattcc
    21901 gatttcataa ccaaattttg aacggctgtc ttcacggatt tctccagtta ctaacacgga
    21961 tgtttcttgg tgcagatttt tagctgtttg gaaaacttct tccggcactt cgctttttac
    22021 aactacacct tggaaaaagg ctgttccgtc tcttaattgt aaaaatgcga tttttccgct
    22081 tgatcgctta tttgcgaccc aagcgccgat tgttactgtt tctcccacat ggtttttcga
    22141 atcgatgatt tgaatttgtt ccacgtatga ctctcctttt atattccttg aatttcttca
    22201 ggattaattt tgacttttac tctcaataaa ttgcttgatt ctgcgtaccg cttcttctaa
    22261 cgtagcgcga tcagtggcat aactcatgcg gacattttct ggtgccccaa agccttcgcc
    22321 agtcacaagc gctacccctg tttcttctaa aagatcttct acccattgtg tgacattttc
    22381 atatttacac atgcgtagcg tttcttttac attaggaaaa agataaaaag ctccttgtgg
    22441 cttttcaaga gaaacacccg gcagttctgc cacaagcgga tataacgtat tcaggcgttc
    22501 ttcaaaggcg ttccgcattt cttccactgt atcttgctcg cctttcaatg cttcaacagc
    22561 tgcatattga ctgactgctg tcggattgct agttgattgg gaagcaacag cgatcattcc
    22621 attgatgatc acaggatttc ccaccgcata accaatgcgc cagcctgtca tcgcatatgt
    22681 tttagacacg ccgttgatga tgatggtctg atttttgatt gcttctgaaa tcgttgcaat
    22741 tggagtaaac tcattgccat tataaactaa acggccataa atatcatcag caactatcaa
    22801 aatatcattt tttactgccc attcaccgat tgcctgcagt tcattcttgc tataaatcat
    22861 tccagtcgga ttcgatggag aattgatgat tacggctttt gttttttctg aacgtgcttg
    22921 ttctagctgc tcaacagtaa ctttccagct attggattct tcccctttca caaagacagg
    22981 aagtccttct gccagtttta cttgctcacc gtaactgacc cagtaaggca caggaatgat
    23041 gacttcatct tgcgggtcaa ggatcgtttg gaaaaaggta tacaaagcaa atttcgctcc
    23101 gtctgttacg actgtttgtg aaggttcata gacaagtcca tagtttttct tcagataatc
    23161 aacgattgcc tgtcgaagct ctgggattcc tgctgttggt gtataatagc tagctttccc
    23221 attgcggatt gcttcgattg ctgcttcttg gatgtttttt ggtgtcgcaa aatccggttc
    23281 tcccacagtc aaactcagca catctctgcc tttggctttt aatgccttgg cctttgccgc
    23341 agctgccaaa gtgacagacg gttctaatcg ttttgttctt tttgataact ccatcccatt
    23401 cattccttta ccttttaaat tcccttgatt gtcttgactt catctccaga ttcaaagcca
    23461 atcaggtaat aattcagttt cccatttgtt tcttttccta ctacttccca cacaggctga
    23521 tcatcataca tccccaaagc agctttttcg atttggattt caggatgggc cgcagcaatt
    23581 gtctgttttg cttcgttttc agtcagtccc tttgtttgat ccaatacttt tactttttca
    23641 ccagatttag gaatgataac ggcgatttct tttcctttat catttgtacc tgtcagtgaa
    23701 aagtatgtct tctttcgagt aaaccaataa aaatgatcgg cttcctcaag atttgcatat
    23761 ttctttgcga tttctactgc ttctttcttc gcctgcaccg ccggacgact ggaacgcaag
    23821 taaaaaatcg agctgatcac gatgaccgct aataaaaata cgatcaagcc aagtaacacg
    23881 cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg gtcgtcgctc ctttatcttc
    23941 attccttttc attatagcag attttaatta tgaaaaaaga cagctcctct aacttccttc
    24001 ttcatttttc tttaagaatt cctggatgtc ctggtagatt tcctccatag ctagctcttt
    24061 gatcggtact tttttcggta atgcatggat gatccgtgca ccatattttg cagtgactag
    24121 tcttcgatcc aaaataagca atacgccacg atctgtctcc gtccttatca agcgacctaa
    24181 tgcttggcgt aacttcaaag ctgctttagg tacggattcc tggtaaaaag gattgatccc
    24241 ctctgattct aaataagcgt tcctagcttt gaccatcggg cgaagcggat tatcgaaggg
    24301 taaacgtgtg actacaacga tctgcaaagc atctccaggc aaatctacac cttcccaaaa
    24361 gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg aagcgtttca aaagcttctc
    24421 ccggcttccg ccaaaccctt gagctagtat ttctcgtcct ttttctagca tagacagatg
    24481 cattttctga tagaccttct gcaaaacatc atgagaagtg aataacacaa gtattggccg
    24541 atcttcttgt tcgatcagac ctgtaagtgc tgtctctaga taatcgatat atgcttgatt
    24601 gctgacttga tggacggcag gtgcctcttt tggcacatag atccttgcct gtttctcata
    24661 atcataagga gaggcaatca ctttaagagg tgctgcttca atgccaagcc gcttagagag
    24721 atagtgtcga ttagggccga tcttcagcgt tcctccaata taaagaatac gatcatatcg
    24781 aggataccaa acagtcgacg gcaacatagc cgcttgaaaa tcatggagat gaaaaatcat
    24841 ctgctgcgct gtgttcccag gaacgaacca atggatcaaa tgatctcgcc agtcttccag
    24901 ccattgatcc atcagcaagt actgcttgtc taattgatca aataagctat ataaacttgc
    24961 taaatcgatc cgcatctggt ttgtccagct ttcttggata atctcaaaag tcagacgcat
    25021 ttgtgactgt agctgctgga tctcctgata aaaaagctgc aggtgatcaa tggctgtccc
    25081 agattccagt ggcagctgat ccaacatctc ttttgtcatc atgacttctt ctggatactg
    25141 tccgttaggt tgcttgccgc tcactaaact cattaatcct tctgccatac aaatctgtgc
    25201 ttcgcatagc gcagaaagtt cttctcgata aatcgataac cagcgtaagg cttcatgttc
    25261 tttcggtaaa tagctttgta ttcggtcaaa cagctgattt ggttcttcta attgctggat
    25321 ttgtttatgg aaatagttaa tacttaatcg tttgtcattt actttttcag cgatttccgg
    25381 caaatgatgt acttcatcga tcaacaaaaa tggactagct ggaagcaatg gttcttttcg
    25441 ctgtgtttct tgtattaaaa acgcatgatt gacaatcaaa aaattacttt gcctgatttt
    25501 cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac tgatttcttg ctaaaaatcc
    25561 aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc aaatttgtta aatgtaattc
    25621 atccaaatca ccggtcttcg tttgcgtcag ccaaactaag accgccattt gatacaaagc
    25681 atattgtttt tgttctggcg gttccacaag tgtgccttta aaacgctgta aatcaatata
    25741 gtggcgatag cttttgataa ttacaggttg tatgggctga tctagcaatt gattcagcaa
    25801 tggaatgtct ttttctatga tctgctgctg caaaactagc gaaacggtac tgatgacagc
    25861 ggttttttca ggtgtagcca aataactcaa tggcagcaga taaccgattg ttttacccat
    25921 tcctgtagct gcttcgatca tcaaatcttt tgattcatct tttgtgaaat ggtcgtagac
    25981 cgcattcatc agtctgttct gttcttttcg atacatcagt gttttaccaa acattttttc
    26041 ttttgcttgt ttttttcttg gatatgtccg ctcgccataa tggacggatg tgaatagttc
    26101 cacttctttt ttctgtaaag ctagcccatc gacgatttct aacgagggat caagcggctc
    26161 tggtttttct ttcatttctt caatcacatg ataaatgaaa cgactcgtat ccatacctgt
    26221 gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt ggcaattttc tcatctgttc
    26281 ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca ctatctgcct gatgaggatt
    26341 ctcatgaata aatccaaggc tttctgacaa atcagctaag cgaaagcttg gttccgtcgg
    26401 caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt atcttcaaac taggaagtcc
    26461 gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa taaatattat gcgcgacaaa
    26521 tatggtggct gcaaaaagat tgtaaatcgt ttgggcgaca tcttcgaaat aaggcgcttt
    26581 ttggacgcgt tgattcgtga tccctgttaa atgctggatt tgttttgaaa tcttccgccc
    26641 tggatgaata tcgatcgaaa aacgtgaagt gatccgacct gattcaatca tgacgcagcc
    26701 aaattggatg atccgatctt cttttggatt cgtcccagtc gtttcgatat caacgactgc
    26761 ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc tctaatgttc ttttgaaatt
    26821 atactacaaa gtgcagaaaa atactctcat cgctttttaa agaaaataga caaaaacgcc
    26881 cagacacgca aatgtctggg cgtttatttt ttagtatagg ttaattaagc tttagtaacg
    26941 tttgttgctt gaggtccgcg ttggccttct tcaacatcga aagttactgc ttggccttct
    27001 tctaaagttt tgaatccgtc gccttggata gctgagaaat gaacgaatac atcgtttccg
    27061 ttttctgcag tgatgaatcc aaatcctttt tctgagttaa accattttac tgtacctgtt
    27121 tccataataa aaatcctcct tgtgtatcaa cacatatttt gcaattactt gaatggtgta
    27181 tatctggaag atgtttattt gaaacaagct accacagatt accgaacaaa aactactcct
    27241 atagtatatc ataccgttta caaaaaagct agagtttttc gacttttttt attaaaaaaa
    27301 gttttctcag cgattacata aagtgaaaaa ccgaactttt tttaaaataa acgacatatt
    27361 gttcagttgg tgtttttttg ctctgatgtt cttttttaaa ctaaaaaaat tattctttct
    27421 gactacttat cggttctgtt tcaacggtcg aggctgggga agattcttta gtaggtctga
    27481 tgttctcatg gtatttctga tagcctatga cagttaccag aagaacaagg caaaccaata
    27541 cgatcaacga gattagtttt tcttttttgc tcatatctat acctcataaa taacaggatg
    27601 atgtccatca tatttctgag tatgagtaaa agcaaaagct gcagctgtag aatgtaagaa
    27661 atgccagcaa ctttcataat cacaggtcgg acagtaccaa aagtaaccaa atcttggctg
    27721 caaagaccgt tcttctgttt caggaatcgc agaatcaaga tctggggtct gtacataaac
    27781 atttttgtac agacgtgtgc ctgtaatagg agaccgctgt gtttcttcag caaaaactgg
    27841 ctgacttgtt aaacttaggg atagaaacag caaacaccat aacagtcttt ttttcataaa
    27901 gacctcctta tcgataagtt tttcccaatt aaaatcatat ctcaagaacg cccgttattc
    27961 acattttgtt cataaatgat gttttttgta tcataatcgt caaaaagagg tcgtgaccac
    28021 gtcacaacct cttttttaaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat
    28081 ttcacaaaaa tttgagaaat aatttttgtc aatctcttct tatttcttga agctgaccac
    28141 ttctgtcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    28201 accaaacaaa aaatatgaag agctattttt gcttgctgtt cttattactt gatgcagaac
    28261 agctttttca caacctctca tacacggtgt ttaatcagct gaccttcggt attagctcaa
    28321 aaaacggcaa aatatgtgaa actatttttc cgtttgtttc tctaatactc aggtcaaaac
    28381 actgatttca caacctcttt taataacgaa gaatcgctgt cagactcttt tcatctggcg
    28441 catctttttg atccaaaata aatactttcc cgccattaat gatcacatca tctgctaatg
    28501 cattcaacac ttgacgccga tcatattcta tttcaggatc ttcaccgaaa ccatcagcta
    28561 agtttgacgt agcgataaat agatgcgaca cttttccttc tttcgcagct ggaacgatat
    28621 caactaactg atcgatgaat ttcttatcta agagtttatt gtattctttt gattcttgag
    28681 ctgcgagttc acttgacagc ttcttcgccc cttcttcgat atctctaaag gatagctgcg
    28741 caggagaagc agaaatcgca acgtctgcac aatagtaagg atttttggcg atttttttga
    28801 acatcgtctg attttctggt agagcaaata aataaatcgg ccattgttct ggattttcga
    28861 aagaatcctt caagtacgta tcaactgctt ggtaatagtt gacccaatcg atctctactt
    28921 cttcatcttt tggatttacg ccatggaaag ttactccatc tcgggctccg cctcctccat
    28981 tagaagagaa gttcaaaccg ccacctgtca gttcagatcc caatgcagta acgacatcaa
    29041 ccggtgcccc ttctggtaga gatacttctt ttacttgttt gttgctgact tcatacaatt
    29101 tcatggagtc gcggttcaat cccaataaat aataagaata gttgaactgc ccatttttga
    29161 taatagccag taaataagga agatcactta catagtattg atcgtctacc gggatactca
    29221 aacgatggat ataggtttgg tcagcagtta taatgaccgc tacactcgta gttgcactac
    29281 gccaaaacga aggatctcct aacaaatttt caattttttc ttggaacggg gaccagtcat
    29341 gttcggaata ttttttctca aaacgtttct ttgcttcttt cgcaaaattt ttgaatttga
    29401 tctgatcttt ttccacatct tgatgggcga catgcgtatt cagcatgatc gtgacgaatg
    29461 gtcctttagc gccttctgaa gtcaattgtg acaataattc tttatccttt tttgccataa
    29521 acgtacccct cctaaaaaaa tcattccttt gtttctatct aaagtctacc acaacactat
    29581 ttttccatac aataaaacgt atctaaaaaa ataaaacggt ttctacggtt ttaaatttag
    29641 ctattttttt aagttatctt cataatttcc tcaattattt agccccagtc ttcaactatt
    29701 cttcatgaaa tgctgataaa ctaagaaaaa gaataaaaga ggtggatctt atgcatttga
    29761 ttatgatttt cagtttatta agtattatca gtttatttgc tttcagcgct tcccgcttat
    29821 tagatgcgga aacacagcct attcctgtaa aagtacgtaa aaacgaccgc taaacttcca
    29881 taacgcgcgt tcttatccta aagttttttc agaaacattg aaactctctc ccctctctga
    29941 taaaatttta atttgtagcg aaacaaatta attacaagga gatcggatat gtccataaaa
    30001 aaaacacgtc tagtcctatt attggctagt ctcattcttt gccttgcttc ttttctacca
    30061 gctgttgctg tgcaagctga agatacgttc aaagtaaatg caaaagcagc ttttgctgta
    30121 gatgcgcaaa gtggtaagat tctttatgat caagatggag aaaaaaccat ggggatcgct
    30181 tcaatcacaa aaatcatcgg tctttacatc gttcttgatc aagtaaaaga aggaaaacta
    30241 tcatgggatg ataaagtttc gatttcagat tatgcagaaa atttgagtat caccccagat
    30301 ttatcgaatg ttcctttgca taaagaaaac acctacactg tcaaagaatt attcgactct
    30361 gcaatcatcc agtcagccaa tgcttcaatg gtggccttgg cagaaaaaat ctctggcagc
    30421 gaagctaaat tcactgaacg aatgaaagaa caattgaaag attggggaat caaagatgca
    30481 acaatcgtta atgcatcagg attgaacaac tcttatcttg gcgaaaaccg accagaaggc
    30541 actggagaaa acgacgaaaa tcaaatgtct gctcaagatg tggccatcgt tgcccgccat
    30601 ttgatcttgg atttcccaga aatactggat gtatcaagta ctacgaccca aatgtttggc
    30661 gaaaatacgc aatcaccagt ggaaatggtc aactggaact ggatgctccc aggattcgtt
    30721 aattacaagg aaggcgtgga cggattaaaa actggtacga ctgagcttgc aggtgcttgt
    30781 ttcgttggta cgatcactaa aaacgatcaa cggatcatca ctgtcgtatt gaatgcggac
    30841 ggccatgcgg aaaacccgag tgcccgcttc aatgaaacag accgtttgat ggactactgc
    30901 tatgacaatt ggtcagaaaa agagctagga aaagctaacg caagtattcc tagcctaaaa
    30961 accattgatg taaaagacgg aaaagaatca acagttcctg ttgtgcttaa aagccctgta
    31021 aaagtatggg tacgaaacga tatggatact ggcaaattaa cgatcacgcc aacacttgac
    31081 aagaagcaag tcactgacaa cgagatccaa gcacctatgg ataaaggtac aaaaatcggt
    31141 accgctacga tcaccttggc agacgataaa ttaggctatt tagaagacaa caatgcgcca
    31201 tcaacagaga tcattacgga taaaacagtg gaaaaagcga atatgttcgt gattggctgg
    31261 cgacgcgtcg cagaattttt cggcaacttg ttttaattga acaataagaa aagcatcttt
    31321 ccatcggaaa gatgtttttt tgctttttct agtttaaata acagcttttt tcatctattt
    31381 tcgttatact gtataagaaa aaggagttga atttatgaat ctaaccagag atctgatctt
    31441 tttagttctc tatcttattg gggtattctc tatcgtttat tggcgaaaac agaagaagga
    31501 tccttccttg ttcttttacg gcagtacgtg gacatttacg gtaatcttaa cttattttgt
    31561 cgtgttggaa attttttatt cgacctttta ttttttgatt ccattgttgt tctttagtat
    31621 ctttgctttt tcttatttca cagaaaaacg aaggctgctg aatggtcttt tattcaatgt
    31681 tttcctcatc agtttcggca tctacttgtt tgtcttgctg tatgaaacgc aaaatatttt
    31741 ccttggcgga ctgatcgcct tgatcacgat tccattgcta cttgtccttt tatttggtat
    31801 ttatggattg attgtttttc ttttttggaa cggggtcaca gttttacgta gagaatcgca
    31861 ttcattagct aatcttttaa cgttgatact agccattttt cttacactgt ttcttgtttt
    31921 tgactttttc ttgttaaaat atttgccgca atggataaat gctttgtttt tctgcgttcc
    31981 cctgatcttg atctatctat ttatcgtatt ctacaacttt ttgactgtct cattcttgta
    32041 tcagttcaac cggcctcgat acaatcaaga cttcatcgtt gtgttaggcg ctgggttgat
    32101 caatggagaa acagtgtctc ctcttctagc caagcgaatc aataaggcaa ttgcttttta
    32161 tcgggctcaa agccgtgcta cattaaatcc gcccatctta ttgatgtctg gcgggcaagg
    32221 agcagacgaa aaagtaccag aggccattgc gatgaaacaa tatgccatgg aacaaggaat
    32281 ccctgaaaga gacattcttg tcgaaaccaa ttcgacaacc actttggaga acatgctcta
    32341 ttcaaaggaa atcatggatc agcaaatgaa agggccatac cgcgcgatct tcagttccaa
    32401 caattaccac atttttagag ccggtcttta tgcgcggcaa gccaaattga aagcaaatgg
    32461 tattggtgca aaaacagctt tctactactt gcctaatgcg tttttaagag aatacatcgc
    32521 catcttgatg atgaacaaaa aaagacatat gatcatttgc ggattgcttt tcgcaggctc
    32581 cgcattctta tcgctgatta ctttactttt ctaaaaaaac aaggtgtgac aaatatatgt
    32641 cacaccttgt ttttttcttt cattctctta actattggca aaaagagtat cccttttttg
    32701 accgctactt gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag
    32761 cagatcataa agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag
    32821 caggcaatcg gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga
    32881 aaaggaacag aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca
    32941 aacaatggtg tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat
    33001 aacttcccaa taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga
    33061 agactcaatc cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct
    33121 ccacctatcg tatttcgcca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa
    33181 aagacgatcg aacggctgaa tacccagcca atcaaaccaa aagcaatccc agcaacaaaa
    33241 actttgaaga acagcaacat actccatcca gggatcacac ccatttggta atgtaggtga
    33301 gaaacaccat agctttccgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag
    33361 atcgcttctg ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct
    33421 aaaggtgtgc caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc
    33481 cgttctgctt tatccaatcg gaacaaacgt gctgtattat ctgctaacgc tccccccatt
    33541 tggactgctg ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca
    33601 aacagtgtaa gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg
    33661 atcactaagt tgtttccacg actagaaaga ccgccgtgat acgcatagag ataagcaaaa
    33721 agcgcaccac taactggtag tagataaagg agccaaggat gtgatagtcg gatatctgtc
    33781 acaactgtca ggctatttaa gaaaaaagca gataagctcc ccatgaatat accgagaatc
    33841 gctgtgatca ccagccactt cagcatgtac actaaaatgc gtactggact cggtaattgt
    33901 ttttccataa aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc
    33961 agactttagc aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc
    34021 tattcacttc cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct
    34081 ttctttacac gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat
    34141 tggtcactat catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct
    34201 ttcgttgtag aaagaaaaag cgaaacatac gatgttttct gacagattgc acagattccc
    34261 ttatttatcg ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag
    34321 accatgaatt ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag
    34381 tcgatttcat tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt
    34441 tgtggtttgc taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt
    34501 tctgcttgcg cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgatttct
    34561 agcagttcgt gatctaatat gtctcgaatc ttttcagctg ctagtgcctg aactgtttgt
    34621 accgtttttg gatcattgac agatgagtac gcatttaata actgttctac ttctcttttt
    34681 ataaaaaaat attggtatgg ttttattgtt tgttccatga ttttaaagtc ctttcttcta
    34741 accaaagtgc atactttgta gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga
    34801 tcccggactc ttttaaataa gatacgtttt gcatcttatc atcaccccct ttaatgctta
    34861 ttcttctatg tacatgattc attcgataaa taaaagtaaa ccgtatcttg aacagatagt
    34921 caactgcaaa cagaaaaaag gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat
    34981 gaatggtgtt caaaagcttg catctgcggt aataagccga aacttcacaa aaatttgaga
    35041 agtaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcaa
    35101 tatggtctat tattggtttt acctgttaaa tcgaggattc tttcccaaag taacgttgcc
    35161 atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg cagtatttgt
    35221 ccacgaatgt cacgatatat ccttctttgt acttaggcgg tgcgatcgtt gcaccccaca
    35281 tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt tctgcattct
    35341 ttacagcaat tcttggatgg atatacgcat gtgttccctc gtcaaatttc gtcgtacgcc
    35401 aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta gcattgccac
    35461 cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc tccaaacgag
    35521 tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt tctaaaagat
    35581 cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata attatcgatc
    35641 tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca ccagtgttcg
    35701 gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta atcataaaga
    35761 aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg catttccttt
    35821 cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta ctccctgacc
    35881 ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta atgcatgtgc
    35941 ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta cagggattac
    36001 atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca tgcttcgcag
    36061 cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag ccataccaaa
    36121 cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat tatctagtaa
    36181 aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca ctgctaagat
    36241 cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa ctagaaaagt
    36301 agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc tctgtgtcac
    36361 aaaaagttct tttatttctg catcagacga cgcagcttct tcgatcagat ggaacccttc
    36421 caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt ttaattcttt
    36481 gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa ctgattatat
    36541 catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt gatttataaa
    36601 acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag gacgtgtcca
    36661 gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg tgaccggaac
    36721 ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg acgacattat
    36781 ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag tgcagtatgt
    36841 ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtgg tgggataagg
    36901 ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat ggctttccac
    36961 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tcgataaggt
    37021 tgtttttgaa gcgctttgcc tcgagtatta aaaacaggta gtaaaaaagc tgatctataa
    37081 cttgcaataa atttgcttaa cccatatttt ccagacagaa caaaaaagtt tttagttttt
    37141 taaataagaa agaaacactt ggaatcactc ttttctttcc aagtatttct ttcttgattt
    37201 tttgtctttt ttcaaaattt ttcgctacga aaaagaaaga atatttatat gaaatttcta
    37261 tgaagttgat tgaattccat gcgctttttt agtatagtta agtttgtgta aaaattatag
    37321 aagaggaagt attctatgaa taaatggaaa aactggttat tgggatccgg acttgtcagt
    37381 atcctgctct ttttatctgg ctgtgtgcgg atggattcaa acggaaaccc tgacacttca
    37441 ggaatcgttt atcgagtact cgtccatcct atgggccaag cgattacata tctagttgat
    37501 aatttcaatt ggtcttacgg ctgggcagtt attgcaatga ctgtgatcgt tcgtatcatc
    37561 atcttgccac taggtatcag tcaatcgaaa aaaacaatga tccaatctga aaaaatgcaa
    37621 gcgttaaaac cacaagttga agcagcacag caaaagctaa aagctgcaac aacacgtgaa
    37681 gaacaaatgg cagcacaagc agaaatgcag caagtatatc gcgaaaatgg attgagtatg
    37741 accggcggaa tcggctgtct gcctctattg atccaaatgc cgatcttctc tgccttgtat
    37801 tttacagcac gctacacaga aggtatccgt gaatcatctt tttacggcat cgatctaggc
    37861 tcaccaagta tggtgcttgt ggccattgca ggggtcgctt atctgcttca aggatatatt
    37921 tcaacaatcg gtattcctga agaacagaaa aagacgatgc gtacgatgct gatcgtctct
    37981 cctgctatga tcgtcttcat gtcgatcagt gcgccagccg gtgttacact ttactgggta
    38041 gtcggtggtg tgttcagctg tctgcaaaca ttcatcacaa acgttatgat gaaaccacgt
    38101 ttgaaagcac aagtggcaga agagttgaaa caaaacccgc caaaacaagt cgtcactcca
    38161 agaaaagacg tgacagaatc agcaaagcca gctaacaaca aacaagcaac aaaacaaatc
    38221 aaacaaccaa aaaacagtac aaacaacaaa ggccgcaacg caggaaagca acagaagcgg
    38281 aagtagatag taaataagag tgtaataaaa attcattaac agttttagca attaaaaata
    38341 tcgaccaaga tttttgaaaa agaaatctgg tcgatatttt tatttaagtg aatgtttaat
    38401 ttgaatcatc gattgatcgc tatctaattg cagcagcatc tttttatgaa gaaacaactt
    38461 tttcttatct atcaacgtag acaggtgttg ctgtttcttc taatagtgta cttaattcct
    38521 taatattttg ttttccttct tctaacatcc aactactcgc tttttcttcg ccttcggtaa
    38581 caaaaggcgc cactcccttc gcccatgttg ctcgcccaca taagacacca ttgaatgtcg
    38641 aacctgattg ttttgcaaaa cgtaatgtat cttgaaatag cgtagcattg actcccgcac
    38701 ttaaaaaaat aaacggttgt tcagtcactt tcgattgttc atagaaatac gcggatgcct
    38761 ctgcttgact ataacaagtc tcttacccat atccttccac aaatctcata ttcactggca
    38821 cttctacttt caatacatcc acttgataac gttcttttga aaattctcgc atggcttcaa
    38881 taactttatg gggttttact tttgcatagg caagagaaga cggatcgcca atagttgcat
    38941 cataagaaag gatctctaaa aagaaaggta tgccttctgc ttcgcattct gaaccaatcc
    39001 gttctataac tatttgtttt tgcacattaa tctctgctgg ttcatccacg tcatagtaca
    39061 ataaaaattt acaagcatcc gcaccagctt cttttaacct tttcaccgac caaatattca
    39121 ataaatcagg taatcttccc ggcattttcg tatcatagcc ggttttttca taagctagca
    39181 gtaagccaca ttctttctct cgaagcgctg ctgcaggaag gccgtattct ggatcaagta
    39241 aaatagctga ggcataggga gtcaggtatt gagaaaccat ctgtttaaat tggatgatgt
    39301 cttctgatgt agcttcttta tattggttga tcattttctt caaagcacca cgctggtcga
    39361 ttgctaacgc gctaataatt ccagcttcat ccgtcaatcg cttcaaacgt tcctgctttt
    39421 ctatactcat cttcttcata tatcttcacc tatttcttta gttaatattg tggctatttc
    39481 tgtttttgat tttgcttttg cttttctcaa ctgttctgta acctcttttc gcatcaatga
    39541 tcgtgcaacg agagacaata atttcaaatg agtcgttcct gcttctgaat ctggaataaa
    39601 taacgcaaaa ataaaatcaa ctgactgtct atctaaactg ttccattcaa ttcctttgtt
    39661 cagtgtgacg ataatcatac taggttcgtt gattgtttga gacttcgcat gagggatagc
    39721 aaaacctgac attattcctg tagtaccttc tttttctctt tccagagttt ttgatatact
    39781 tctttttcgt catgagcaat tcctaagcag aatgtttgct ttgctaaaaa cttaaatgct
    39841 tcttcttgac tatccaatgg ctgttgtaaa aaaatgttct cttcatgaat gattgtcatg
    39901 ataagtctcc tgctcttcct ttttcgataa ctgtacgatt tgctgcttta ctatccatct
    39961 tcttgtcttt atcagttact cttttctttt ggattaatcc tagtatcact ccaccaacga
    40021 tcgaaccaat aacaatcgct gtaacccatt ggatttttcc cgtcactaca ggtaatacta
    40081 aaaaaccgcc atgtggtgct agaacttgga cgccaaacaa ataagtcaag acagcagcaa
    40141 ttgatgagcc taacatcatc gtgggtaaca ctcggatcgg gtctttcgca gcaaacggaa
    40201 tagctccttc cgtaatatga gttgatccta aaataaaatt aaccaaacct gcattacgat
    40261 cattggtatc aaaatattta ccaaacaata aagtagcaaa accagtaatc aacggtggtg
    40321 caatacaagc tgctgacaca ccagccataa aagtcgtgtt tccttgtgcc aaaagagctg
    40381 ttcctgttac atatgccgcc ttattaaccg gtcctcccat atcaaacgca cacatgcagc
    40441 caacaatcac gccaagaaca agtggattcg aatgttcaaa tccagctaaa aagctcatca
    40501 ttccttcatt tatagccttc attggacctg ccagcaaaga catcacgatg ccaataaatg
    40561 ctacaccaat aactggatat aagaagattg ccttcaagcc atctagttgt ttaggtaaaa
    40621 ttgtcagtag cttttgcaaa aataagataa aataacctgc taagaaccca gctaaaatgc
    40681 ctcctaaaaa tccagtaccg ccgttcatgg acacgattcc accaacaaat ccaacgacta
    40741 accctggacg ttttgcgatc ccttctgcaa tataagctgc taaaactgga accatcattc
    40801 ccattgctgc atcgccaact acttttacca ttgccgctgt agcattgtat tgtgcatgat
    40861 ctggatcaaa agaatagatt ccccaaactg caaatgaaat agcaatcaat actccgccag
    40921 ctaccacgaa aggcaacata tgagagacac cattcatcaa atttttatag atgctacgac
    40981 caaaattagc accactcatc gcttctggtt ctaaagtatc agcagcttca ttatctgcta
    41041 agacagtacc ttttcctgcc aatgcttcgt caatcagccg ttgtgcatcc ttgattcctg
    41101 tactgacaga aacatcaatg actcgttttc ccgcaaatct ttctgaatgg acatctttgt
    41161 ctgcggcaat aatgacagca tctgcctgtt tgatttcttc agccgttaat tcattttcga
    41221 tcccaatttg tccatgggtc tctactttga tcgtgatgcc ttttttccta gctgcttgtt
    41281 ctaatgcctc ttgcgccatg taggtatgcg caattcctgt cggacaaccg gttgctgcaa
    41341 ttagttgata ttttcccatc tttcgaacct ctcttccctg attttgattt gtttacttaa
    41401 ttcctccaca tctaaaaaat ctgttaatcc tttgcgaaaa gcggttgagc tgcctgctgc
    41461 tatgctttta cgtaacgttt catcaagcgg tcgtcgattc atgtatcctg ctaaaaaagc
    41521 gcctaacatg gtatcacctg cacatgctgt attgacaact tttccttttg gcgagtttcc
    41581 tctaaacacc ctgtctttcg taaaaagaat cgctcctttc tcaccgaggg ataacaaaac
    41641 attttctgca cctaactcta ctagtcgttg tccataaaca aagtaatctt catctgtaac
    41701 catttttcgt ccgaaccaag aagaaagttc ttcctcgttc ggtttcagca aaaatggtcg
    41761 atagggtaaa caatccatgg cttcttgatc actactatca ataatcaaat gaatcccctt
    41821 ttcctgacaa atccgactga tttctattaa aacctttgga gacagccctc ttggtaaact
    41881 cccagaaaca caaagataat cttctgcttg taatactctt atttgattta aaaaattatg
    41941 gacagctgcc tgcggtattt caggtccttg attcactaat ttgtattctt ctcccgtttg
    42001 attgacttgt gtaaatacat tgatacgcgt cataccaggc acttcaataa actctgtttg
    42061 gatttgtttc tgctgtaaat agtcttctat atactttcct gtgaacccag cagaaaaacc
    42121 taatgccgta cttgggaaat ctagcatctt taatactagt gacacattca ccccttttcc
    42181 gtttgcctga atgtcatcct cttttgtgcg gtttaccata aaaggatgca tctcctctgt
    42241 ttcaataaat agatcaattg ccaaattcat cgtgcatgtg tagatagaca taaccaacga
    42301 cctccttgac tctattatga aatacgcgaa agagaaaatg ttttcttaat cgtcccaaca
    42361 caaagaaaac ctttacattt ataaactcgt cgtgctattt tttaactaac ttttggtgaa
    42421 agaaggaaag aatatggatc acaaattaag tgaaacagaa cagtacctct ggaactttat
    42481 cgaacaccac atcctagaga tccctaacta ctccatcgtg aaactaagtg aacaagcaaa
    42541 tgtctcaaca gccaccattg tgcggacaat gaaaaagaaa ggctatgaag ggtttacttc
    42601 gtttaagcat catctaaaag aaggagaaaa caagaacatc aatttttctt tcttagataa
    42661 agtagacaaa ggtatccgcc gagccattct aaaaaacgaa caagaagtgg tacgtacaat
    42721 caatatgttg gaaatcggaa acattgagga tgcgatacaa aaaatcaaat ttgctgaccg
    42781 ggtttttgtc tttgcccgag gattttcaga aatgattggc caagaaatgc tggtaaaact
    42841 tcaattaaca ggaaaaaatt gtcaaatgca tactgatcca gagattatca aaagcatcag
    42901 tcggaaacta actaagaaag acagcgttat ttttgtttct ttgaacggcg agacaaaaga
    42961 actcgtcacc gccgcaaaga attgctatga tgcagagatt ggttcaattc tagttacagc
    43021 caatcattcc tctacactca aagattattg cgagatcaat ctcgtaggtt tcaaatcaga
    43081 aggttcttat ttccctgatt atgaagtacg gtcacgtttg cctctccaga tcatcgcgag
    43141 aatcctgctt gacgcctatg tattgcgtat gggggagaat tgaggatatt tgatttgatt
    43201 gataacaatt gctagaaaaa ctgtaaaaaa tgccttacgt atctcgctac tataatttat
    43261 tagtctcatt ttccatttta gataaaagct atcgtgtact ttagcatggc taaaaatact
    43321 acaaaataaa taaacaaaag gaaataataa aaaaatctgg aacagaagct tgtgctttct
    43381 gttccagttt agggatgatg aaaattcaca tttttcaatt cttataagct atttaaatca
    43441 ctttcttatt gaacaaagtt ctaattatca acttacttgt aaaaactatt ttttccttaa
    43501 taaatcaagg aacggat
//
LOCUS       DAGCXG010000016        42475 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGCXG010000016 DAGCXG010000000
VERSION     DAGCXG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.016"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            240..464
                     /gene="nrdH"
                     /locus_tag="MB022_000859"
     CDS             240..464
                     /gene="nrdH"
                     /locus_tag="MB022_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBT4465874.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            623..2782
                     /gene="nrdE"
                     /locus_tag="MB022_000860"
     CDS             623..2782
                     /gene="nrdE"
                     /locus_tag="MB022_000860"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBT4465875.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2832..3797
                     /gene="nrdF"
                     /locus_tag="MB022_000861"
     CDS             2832..3797
                     /gene="nrdF"
                     /locus_tag="MB022_000861"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBT4465876.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3886..5025)
                     /locus_tag="MB022_000862"
     CDS             complement(3886..5025)
                     /locus_tag="MB022_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4465877.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5334..6047)
                     /locus_tag="MB022_000863"
     CDS             complement(5334..6047)
                     /locus_tag="MB022_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBT4465878.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6301..7002)
                     /gene="nagB"
                     /locus_tag="MB022_000864"
     CDS             complement(6301..7002)
                     /gene="nagB"
                     /locus_tag="MB022_000864"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBT4465879.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7236..8768
                     /locus_tag="MB022_000865"
     CDS             7236..8768
                     /locus_tag="MB022_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4465880.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8959..10161)
                     /locus_tag="MB022_000866"
     CDS             complement(8959..10161)
                     /locus_tag="MB022_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4465881.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10243..10932)
                     /locus_tag="MB022_000867"
     CDS             complement(10243..10932)
                     /locus_tag="MB022_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4465882.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(11038..11406)
                     /gene="rplL"
                     /locus_tag="MB022_000868"
     CDS             complement(11038..11406)
                     /gene="rplL"
                     /locus_tag="MB022_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBT4465883.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11459..11962)
                     /locus_tag="MB022_000869"
     CDS             complement(11459..11962)
                     /locus_tag="MB022_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBT4465884.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(12166..12293)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12372..13061)
                     /gene="rplA"
                     /locus_tag="MB022_000870"
     CDS             complement(12372..13061)
                     /gene="rplA"
                     /locus_tag="MB022_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBT4465885.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(13165..13587)
                     /gene="rplK"
                     /locus_tag="MB022_000871"
     CDS             complement(13165..13587)
                     /gene="rplK"
                     /locus_tag="MB022_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBT4465886.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13930..14598
                     /gene="sdaAB"
                     /locus_tag="MB022_000872"
     CDS             13930..14598
                     /gene="sdaAB"
                     /locus_tag="MB022_000872"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBT4465887.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14611..15483
                     /gene="sdaAA"
                     /locus_tag="MB022_000873"
     CDS             14611..15483
                     /gene="sdaAA"
                     /locus_tag="MB022_000873"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBT4465888.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15574..16593)
                     /locus_tag="MB022_000874"
     CDS             complement(15574..16593)
                     /locus_tag="MB022_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4465889.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16766..17671)
                     /locus_tag="MB022_000875"
     CDS             complement(16766..17671)
                     /locus_tag="MB022_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4465890.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(17730..18275)
                     /gene="nusG"
                     /locus_tag="MB022_000876"
     CDS             complement(17730..18275)
                     /gene="nusG"
                     /locus_tag="MB022_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBT4465891.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18376..18546)
                     /gene="secE"
                     /locus_tag="MB022_000877"
     CDS             complement(18376..18546)
                     /gene="secE"
                     /locus_tag="MB022_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBT4465892.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18570..18722)
                     /gene="rpmG"
                     /locus_tag="MB022_000878"
     CDS             complement(18570..18722)
                     /gene="rpmG"
                     /locus_tag="MB022_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4465893.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18884..19528
                     /locus_tag="MB022_000879"
     CDS             18884..19528
                     /locus_tag="MB022_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4465894.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19584..20087)
                     /locus_tag="MB022_000880"
     CDS             complement(19584..20087)
                     /locus_tag="MB022_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4465895.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(20100..20146)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20684..21649
                     /locus_tag="MB022_000881"
     CDS             20684..21649
                     /locus_tag="MB022_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBT4465896.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21748..22920)
                     /locus_tag="MB022_000882"
     CDS             complement(21748..22920)
                     /locus_tag="MB022_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465897.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22907..24052)
                     /locus_tag="MB022_000883"
     CDS             complement(22907..24052)
                     /locus_tag="MB022_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465898.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(24066..24990)
                     /locus_tag="MB022_000884"
                     /pseudo
     CDS             complement(24066..24990)
                     /locus_tag="MB022_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            25107..26186
                     /locus_tag="MB022_000885"
     CDS             25107..26186
                     /locus_tag="MB022_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4465899.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            26218..26811
                     /locus_tag="MB022_000886"
     CDS             26218..26811
                     /locus_tag="MB022_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4465900.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(26921..27148)
                     /locus_tag="MB022_000887"
     CDS             complement(26921..27148)
                     /locus_tag="MB022_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4465901.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(27251..29839)
                     /locus_tag="MB022_000888"
     CDS             complement(27251..29839)
                     /locus_tag="MB022_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBT4465902.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(29859..30518)
                     /locus_tag="MB022_000889"
     CDS             complement(29859..30518)
                     /locus_tag="MB022_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4465903.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(30567..31070)
                     /gene="trmL"
                     /locus_tag="MB022_000890"
     CDS             complement(30567..31070)
                     /gene="trmL"
                     /locus_tag="MB022_000890"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBT4465904.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(31291..32157)
                     /locus_tag="MB022_000891"
     CDS             complement(31291..32157)
                     /locus_tag="MB022_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4465905.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            32266..32907
                     /locus_tag="MB022_000892"
     CDS             32266..32907
                     /locus_tag="MB022_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBT4465906.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(32969..34330)
                     /gene="mgtE"
                     /locus_tag="MB022_000893"
     CDS             complement(32969..34330)
                     /gene="mgtE"
                     /locus_tag="MB022_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBT4465907.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(34348..35247)
                     /locus_tag="MB022_000894"
     CDS             complement(34348..35247)
                     /locus_tag="MB022_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4465908.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(35249..36046)
                     /locus_tag="MB022_000895"
     CDS             complement(35249..36046)
                     /locus_tag="MB022_000895"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBT4465909.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(36024..36728)
                     /locus_tag="MB022_000896"
     CDS             complement(36024..36728)
                     /locus_tag="MB022_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBT4465910.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            36859..37434
                     /locus_tag="MB022_000897"
     CDS             36859..37434
                     /locus_tag="MB022_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBT4465911.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            37552..38229
                     /locus_tag="MB022_000898"
     CDS             37552..38229
                     /locus_tag="MB022_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4465912.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(38308..40116)
                     /gene="pepF"
                     /locus_tag="MB022_000899"
     CDS             complement(38308..40116)
                     /gene="pepF"
                     /locus_tag="MB022_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBT4465913.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(40292..41290)
                     /locus_tag="MB022_000900"
     CDS             complement(40292..41290)
                     /locus_tag="MB022_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBT4465914.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(41516..42169)
                     /locus_tag="MB022_000901"
     CDS             complement(41516..42169)
                     /locus_tag="MB022_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBT4465915.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        12846 a         8832 c         7490 g        13307 t
ORIGIN      
        1 gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca attttttctc
       61 tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc aaaaacacaa
      121 aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt gactttttta
      181 ctaagagttt tatactatct attatagata ataagaatgt gttagaggag gcactcacca
      241 tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc aaacggtttt
      301 taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct gaagcagtag
      361 actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat gcagaaacag
      421 ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac taaaagaatc
      481 aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc gcaaacttcc
      541 aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat catcaaatac
      601 gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca gttatttcaa
      661 gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata aggataaaga
      721 agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt ttctatctat
      781 tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat tcattcaatt
      841 gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac atttttcttt
      901 caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa ccaacgacgg
      961 ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt actttgcgaa
     1021 cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac gatatcagcc
     1081 agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg tgtcttgttt
     1141 cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact ctgctttgca
     1201 attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg aagccggcgc
     1261 tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga aattattcga
     1321 agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg tggtttattt
     1381 gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa atgcagatga
     1441 aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat tttatgaatt
     1501 gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa aagaatatgg
     1561 tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg caaatccaaa
     1621 cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa aattacaaca
     1681 agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga atccaatcga
     1741 tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa cgccttctgt
     1801 gattaacggc aaacaggaat atgaagtatt aggaacggat atctcatgta acttaggctc
     1861 gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc gtacgatgac
     1921 tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac cgatccaaaa
     1981 cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca cttatcttgc
     2041 aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata tttacttcat
     2101 gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa gaaaacaagt
     2161 tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa aatacgtaac
     2221 tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca tttttatccc
     2281 aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac tgtatcatca
     2341 aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata cgagtgcaag
     2401 tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg gtaaaattta
     2461 ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg cttatgatat
     2521 ggacatgcgt aaagtaatcg atgtttatgc aatggcacaa caacacgtcg atcaaggcat
     2581 gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat ggaagacaac
     2641 aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc acaaaggaat
     2701 caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg gaagcaatca
     2761 atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat tttaattaaa
     2821 ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa attgaagata
     2881 tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat acacgtatcc
     2941 cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa gatacagtag
     3001 gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt ggcatggatc
     3061 agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat attcaattca
     3121 tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat acgaaaaaag
     3181 aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa aaagctgagc
     3241 gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc agcgtctttt
     3301 tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta ggaaacaaca
     3361 aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct gtacatggaa
     3421 cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca gaacaagaaa
     3481 cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa gaacgttata
     3541 cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc ctacgctata
     3601 atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat actgccaatg
     3661 atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat gacttcttct
     3721 cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac gacgattatt
     3781 tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa taaaaagagt
     3841 ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt cacttgtttc
     3901 ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc tcaataaata
     3961 gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta tgcaaatcag
     4021 catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga tcgtcaaagg
     4081 atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat ctaattgttg
     4141 gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa acaaaccgac
     4201 aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat tcgtgattcc
     4261 gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg ccaaaaagct
     4321 cattacacca ataatcaaag catctaagat cgtactgctg atataggcag ataatgtttc
     4381 gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa agaagtgaga
     4441 caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa atggaacggt
     4501 aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac ttgcagataa
     4561 actgactaat atgatattga acaaattact aagagataaa tttgctgatg ctaattgttc
     4621 ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc atttgcttgt
     4681 ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca ttaactgagg
     4741 cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg tcaataaaaa
     4801 accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat cgaacatata
     4861 atagataaaa ccagcaacaa gcaatggtaa aaaaactgcc gaaagcattt tcaatattgg
     4921 actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga gtactagctc
     4981 cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc tcctccaagg
     5041 tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat tttcatttgc
     5101 ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt aaacaaaaac
     5161 acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata aacggtgttc
     5221 aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa ctatttttat
     5281 ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat catttattgc
     5341 gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc ctcgatcaca
     5401 tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta cactcggtag
     5461 tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt ctcttcaaaa
     5521 taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc taacttttta
     5581 gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat cccgataaaa
     5641 ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc agcaattgcc
     5701 cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat gatcgttccc
     5761 gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc atctgaacga
     5821 atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact agcgatctca
     5881 acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt acgcatacct
     5941 gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag catagcgatc
     6001 cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta aacaccctcc
     6061 agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt acctcttacg
     6121 aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac ggtgttcaat
     6181 cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt tttcctttgt
     6241 cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta ctattgaaaa
     6301 ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt gattttgcaa
     6361 tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga ttgcatcagc
     6421 tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg aaccgattcc
     6481 cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt tgtttgcatt
     6541 gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag caccaggttc
     6601 attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga ttggatgtgc
     6661 atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt ttccatctgg
     6721 tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga aatgacggta
     6781 gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag aagtcatttc
     6841 actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg tgcttcctgt
     6901 tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt caaatgcttt
     6961 ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg ctcctttata
     7021 ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta gtatagacca
     7081 aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt ttcataagat
     7141 ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact gtggacgctt
     7201 tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca ttaaacaaca
     7261 ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa ttaaatgctg
     7321 gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg attaaaatca
     7381 tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat attcacaaag
     7441 gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa tttctaaaaa
     7501 ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac caaacatata
     7561 gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg gaattagacc
     7621 aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg ttggggatcg
     7681 atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt tcaaaggtga
     7741 ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa ccaaccaatc
     7801 acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt gacggtgcgt
     7861 atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat atcgcagaca
     7921 tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg aaacaaaagg
     7981 aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa atcgaaaaga
     8041 ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca aaaagccgac
     8101 aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt aaagctgtct
     8161 ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact gatttagtca
     8221 tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt ggtgaaaata
     8281 ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca ctgatcggtg
     8341 agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc aattactttt
     8401 cacaagatct gtactgggaa aatccattag aaacaccact acaatattta agcagtcaat
     8461 ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg ttagttaatc
     8521 aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag gtgaaacttg
     8581 cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg aaccatatcg
     8641 atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga acagtaatca
     8701 tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt gagattgaac
     8761 caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag ttttgtcaca
     8821 actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag ctcaaaaaac
     8881 ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc agaacagctt
     8941 ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt taaaaacaac
     9001 tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta cacctaacca
     9061 gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc caataaagta
     9121 cataaacata aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg
     9181 tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag
     9241 caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac
     9301 aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg
     9361 tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca
     9421 attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc
     9481 aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg
     9541 cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg
     9601 cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata
     9661 gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat
     9721 ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc
     9781 tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa
     9841 agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc
     9901 agataacgct gccattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac
     9961 taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc
    10021 acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat
    10081 attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg
    10141 ttttttttca ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg
    10201 aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt
    10261 tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac
    10321 acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca
    10381 cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag
    10441 aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat
    10501 atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg
    10561 attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat
    10621 gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa
    10681 accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt
    10741 caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc
    10801 tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa
    10861 ccgatagccc actaccagta tcagaatgat tcccaacgag atcaatatca atcggattat
    10921 tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata
    10981 aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta
    11041 tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc
    11101 tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt
    11161 caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc
    11221 tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc
    11281 agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag
    11341 gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa
    11401 tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt
    11461 aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga
    11521 ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg
    11581 ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt
    11641 ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt
    11701 cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag
    11761 cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat
    11821 cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga
    11881 ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt
    11941 ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt
    12001 tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca
    12061 cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct
    12121 ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa
    12181 actctatgcc accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta
    12241 ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact
    12301 agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt
    12361 ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg
    12421 atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca
    12481 tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt
    12541 ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg
    12601 tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct
    12661 tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca
    12721 actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg
    12781 aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca
    12841 gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct
    12901 tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg
    12961 tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact
    13021 ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg
    13081 tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga
    13141 agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc
    13201 ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc
    13261 agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc
    13321 tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt
    13381 aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc
    13441 ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg
    13501 acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt
    13561 aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta
    13621 attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata
    13681 atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt
    13741 tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa
    13801 tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa
    13861 agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag
    13921 gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg
    13981 attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt
    14041 atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc
    14101 tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat
    14161 gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt
    14221 gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga
    14281 acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa
    14341 ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa
    14401 gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc
    14461 ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta
    14521 gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc
    14581 gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat
    14641 caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt
    14701 ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa
    14761 tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca
    14821 aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa
    14881 gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct
    14941 acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg
    15001 cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta
    15061 gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt
    15121 tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa
    15181 gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt
    15241 gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt
    15301 atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt
    15361 gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt
    15421 ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg
    15481 tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa
    15541 gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac
    15601 ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg
    15661 ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg
    15721 gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg
    15781 gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa
    15841 tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat
    15901 accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac
    15961 gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg
    16021 ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg
    16081 ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc
    16141 ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc
    16201 ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg
    16261 gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata
    16321 agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc
    16381 tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc
    16441 aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat
    16501 gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc
    16561 accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta
    16621 ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga
    16681 tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa
    16741 catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat
    16801 ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt
    16861 cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg
    16921 gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag
    16981 tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag
    17041 caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa
    17101 aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg
    17161 ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat
    17221 ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc
    17281 actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag
    17341 acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta
    17401 ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc
    17461 tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg
    17521 ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta
    17581 acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga
    17641 ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa
    17701 tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca
    17761 agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct
    17821 ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg
    17881 acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag
    17941 atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca
    18001 acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc
    18061 atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt
    18121 ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc
    18181 atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta
    18241 tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa
    18301 ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac
    18361 gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat
    18421 tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg tatctttacg
    18481 gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa
    18541 tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga
    18601 ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact
    18661 gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc
    18721 atttacttct gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact
    18781 ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat
    18841 taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact
    18901 tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt
    18961 aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat
    19021 tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat
    19081 gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc
    19141 tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga
    19201 aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg
    19261 gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt
    19321 ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga
    19381 tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc
    19441 gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat
    19501 tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg
    19561 ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa
    19621 taatacatca atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca
    19681 taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg
    19741 ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt
    19801 tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc
    19861 atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc
    19921 aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc
    19981 cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca
    20041 tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt
    20101 ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat
    20161 aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag
    20221 cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg
    20281 gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga
    20341 aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct
    20401 agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt
    20461 aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt
    20521 tcctagactt ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac
    20581 aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag
    20641 gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca
    20701 tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt
    20761 accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca
    20821 cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga
    20881 acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca
    20941 tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt atattccagc
    21001 aggttctgga gatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact
    21061 tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa
    21121 aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat
    21181 cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc
    21241 attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct
    21301 ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa
    21361 gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt
    21421 cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt
    21481 acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt
    21541 acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg
    21601 accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt
    21661 ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt
    21721 tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata
    21781 gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa
    21841 gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact
    21901 gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac
    21961 catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat
    22021 aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc
    22081 cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt
    22141 tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa
    22201 cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat
    22261 gggcgcactc tggtttcgtt tactgatctc tttgcgatta aatgctagat tgatgacgct
    22321 gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt
    22381 caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga
    22441 gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag
    22501 aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc
    22561 tgggcgactc tgtatcttga tcgtcggcat ctttcctact tcgacttgtt cggtaaagtc
    22621 tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt
    22681 ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc
    22741 atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt
    22801 attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc
    22861 tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat
    22921 aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca
    22981 accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac
    23041 tttcgctgat caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc
    23101 aatacttcac ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg
    23161 aaatcaagat gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg
    23221 tacctaacaa aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac
    23281 gttcaccaaa gtctacttga tagataaaac gaaagacagc taacataata taaatctctg
    23341 tcaaaacaat cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag
    23401 gaatcaagga caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc
    23461 ggacccccca catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac
    23521 ttttgaaact catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa
    23581 cagtcatgat gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac
    23641 caatacgctg taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt
    23701 gacccacatg gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg
    23761 catttagctt ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat
    23821 cattgacttc gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg
    23881 ctttgattac aattccagta ttaatcgttt cgagattgcc accttgatca atctcgccaa
    23941 aggccgccat aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg
    24001 atttatttcg cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc
    24061 ctcctctagt ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct
    24121 tctttggttg ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc
    24181 aacaatactg tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt
    24241 ccatcagaag ctattgttcg ctgacaacac gttttctggg agtaatggga tgtcgatcgt
    24301 caaacgatcc gccgtccgta ccatatcttt tagagattct ttggttcctt tagcaatcac
    24361 ctgtccttga tccatgatca cgatctggtc gcaaagaatt tctacttctt ccatgtaatg
    24421 tgttgtatag acaatggttg ctccttgtcg attcagctcc ttgatacttt ctaaaatttt
    24481 attccgactt tgggggtcta cagctactgt cggctcatct aaaaaaatta accgcggctt
    24541 gtgggcaatc ccacatgcga tgttcaaccg acgtttcaag ccaccactaa gttctcctgg
    24601 acgaaacgaa acatattttt ctaatcccac taattgaatc acctcttcaa tcaaggcttt
    24661 ccttttctct ttttcggaaa catataaacc gcaaaaataa tcgatatttt cttgcacact
    24721 tagttcgtca aagaccgcaa cgtcttgagg aacgacccca atcattgcct tgacagcata
    24781 attttcagga gtcattgttg tcccgaacaa tttaatggtt cctttgtcat aatttaacaa
    24841 agaaagcata cagttgatgg cagtcgattt cccactgccg tttggtccta acagacctaa
    24901 gacttctcct tcttgtactt gcagatcaaa atgattcaat gctacaaaat ctccatagcg
    24961 tttgactaat ttatcaattt cgataatcat catgaaaaac tccttccaaa tttcttgttg
    25021 atctgactat aataaagtgc aagaaaagaa actagtgacg attggctaaa ccacacatga
    25081 catttgtcac aatgaaggag tctgctatgc ataaatctcg gctactcaac gaaactagct
    25141 ttctactggt tatctcattg cttttgctgg taagtcaagg attcgttgct atgcaagtta
    25201 tctatttatt gatcgccgtt ttattcactg gtgtcagtta tttactgcct acaaagccaa
    25261 attggatttt ccaagttatt ctatttcttt tatgtgcctt tttacctgat cttgtcctgt
    25321 ttctgccagc gacgatgcga gtgatcgccc ctgaagaaag ccaccttgat tacttgtttt
    25381 tgttaggttt tttgttgatt tcgacggttg gtaatcatcc ccccatcaag gtcgcctttg
    25441 tcgtaatttt gctgtttttt tccttttact tacgaaaaaa agatcaacga acgaccgatc
    25501 ttgagaaaaa gtacattgtc tccaaagata ctcattggga aaaacaacaa gagttatcgg
    25561 aacaaaataa agcattggcc ctgtcacaag aaacacaatt agccttgcaa attgtggaag
    25621 aacggaaccg aattgcccga gacatccatg acaatgtcgg tcatctgctg tcaagtgcga
    25681 ttttacaaat tggtgcgctt gaaatgatca atcaagaacc aaaactaaaa gaaccattag
    25741 aaaatttaag tgccaccgtt cacactggga tggatcggat tcgtcaaagt gtccatgacc
    25801 tccatgagac atcgatctct tttcaacact ccttagagtt tctgatcgct gattttcctt
    25861 cttctgttac gattgaagga aacttgtttg aaggattgaa tgaaacgcag caaaactgct
    25921 tcttaatggt catcaaagaa gccttaacca acagtatcaa acatagtcat gccaaaaccg
    25981 ttgttttaca ttttcgaacg ttgccggcct tttatcgctt gcaaatcacg aatgatggca
    26041 ccagcccaca aaaaaattct gctcccggta ttggtttgaa aacgatgcgt caacgtgttc
    26101 aagaacaaaa gggacaagtc catattttcc aagaaaacca gcaatttcag ttaacgatca
    26161 ttttaccaag ggagggacac caatgataac agtagtcatt attgagatga tccatttgtg
    26221 acgaaatcgt tacaaaccat tttagaaagc acgaaagaga tccgtgtgtt aggcattggt
    26281 cattgtgcca aggatgcgct agtcttatac gaaacccatc gtcccgatgt tttgctgacg
    26341 gacatccgca tgcctaaaca aacgggcatc gatgctgcca aagaaatttt ggctaaattt
    26401 cccaaagcca tccttttgct tttgacgaca ttcaaagatg aagaatacat ccgcgaagca
    26461 ttttccatcg gtgtcaaagg gtatttaatc aaacagaact tgcaagcgat tattccttct
    26521 gtcaaatccg cttataatgg acaggccgta tttggtaatg aaatcatcga gactttcact
    26581 caaatgatga agaataaacc gacgattgat agtcatgctt atgcttcttt tagtgaacgt
    26641 gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca ataaagaaat tgcagatgcc
    26701 ttgtacttga gtgacggcac tgttcgcaat tacatcagcc aattactaga aaagctagat
    26761 ttacgggatc gcacgcaatt ggctatctat ttttatcagc atttgcaata agtcttatct
    26821 aacacaaaaa aacctgaatg atgctccttc gcatcattca ggtttttttc acatcttcta
    26881 ttcaattgtc ccgcaaacca tttcctcacg attgaaagaa tcaaggattc gcagggctgc
    26941 caggattcgt tggattgttc ggcgtgttag gcggaaaaat aggcgccttg tctaacgttt
    27001 tttctagttt tgattcttca cgatcgtctc gttctacatc aggactttca ttcaatttga
    27061 attctttttc catctcatct aactttttaa agtcattgcc agattctttt gctaatttcg
    27121 ctaatttatc ttcggtttct ttagccataa agtaacgccc cccttttgat ttcagcttag
    27181 catatcacac tgaaaaaaca aaagaaaacg ctaaccaacg atcattttta gctatttctc
    27241 atctttaaaa tcaagcattt tttgccggta taaaatcgta gggagaactg ttttccatac
    27301 caatcatcgg atctactaac tggttatcga tcacttctgc tgtaagaacg aaatctttag
    27361 aaacagcagg ttctacctct tctttctttt cttctccaac aaatgaagaa aacagatctg
    27421 gttccactgt ttccattact tggtttttcg tttcattcgt cccactgtca tcttctacag
    27481 gtagcttttc ttttacaacc cgtggctctt ttgtatagtt cccatcttca aatggatcat
    27541 ttccgaccaa agaatcttca taagctgcta gtttggtccg gagagtgata ttcattttat
    27601 cattatccat gatccttgat ttcaacgtag tcatccggct tgctgattca ttcgctacac
    27661 aagtgacata ggcttgctct tcccctaaca aaatcaacag ttccttactg cgagtcactg
    27721 ccgtgtagag caaattacgc tgaagcatcc gctgatattg atggaccatg ggcaaaataa
    27781 ccatcttgaa ttcactgcct tgtgatttat ggattgagca acaataagaa agagtgattt
    27841 tattccattc gtttctttta taagtgacct catttgcatc aaactggata actaattcat
    27901 ccactttatc ttcagaatct tttgccaaga tgatacccgt gatttctccc atatctccat
    27961 tgaatacatt caattcggga gtattgacta attggaggac tttgtctcct atccgataaa
    28021 cggtgtcgtt ccaatgtact tccttttttc caggtttccc tggattgaat atctcctgca
    28081 tcattttatt taacgcatcg atccctgcag gtccccgata cattggtgct aatacttgga
    28141 tatcttgtgc acggaatccc ttatttttcg ctttttccac aattttagag atcaagggtt
    28201 caatttgata agcatcactt cggaaatagg aacgatcttt ttgattcttt gtgaaatctt
    28261 ttggcagacg tccttctttg atctcatggg caagaggaat aatactagaa ccatcacctt
    28321 gccgataaat ctgattgagt tccattttgg gaatttgttc gatttccaaa agatcatgga
    28381 gtacttgacc aggaccgaca gagggaagct gatctttgtc tcctactaag ataacttgca
    28441 tattcgtcgg aatcgctttg aataatgtgt tagccagcca tgtatcgacc atagacatct
    28501 catcaacaat caaaagtccg ccttctaatt ctttcgtact gactgaaggt gttttttccc
    28561 gtccattcag ccccagaaga cgatggatcg tactggcagg cagacctgtc gtttcgttca
    28621 ttcgtttagc tgctcggcca gttggtgctg caagtagaat aggaaaaatt tcttgggtat
    28681 attttgaagg atccaattcc agtccgttca actctgcaaa aagattgacg attccgttga
    28741 tcacagtagt tttccccgta cctggtcctc ctgttaaaat aaatagaggg gaacggattg
    28801 cttcagcgat agcttgttct tgcgaaggtc catactgtat gccgaatatt ttttcgatac
    28861 gccgaatatt cttctgcact tctttttcag gataatggat ttctttttta cgactgagaa
    28921 ggcgctggat cgaattaccg atcccccatt cagaaaagta caaactgttt tcgtaaagat
    28981 tagtctcttc ttgttgtatc tttccttctt caacaagttg gataattaca gtagcaacct
    29041 gatcaggctg gatttcaatt gggcgacttg tttctaaaat acggatcgtt tgctgaagca
    29101 atgtctccgc cggtacgtat gtatctcctg tttgaactgc atgctgaaaa atctgatgca
    29161 agatagctgc tcgaattctt ctcggtgaat ctgctgcaat accgatttgt tcggctagat
    29221 tatctgcacg tttgaacccg attccttcta tgtcttcgac tagctggtac ggattttctt
    29281 cgatgatatc caaagactcg ttcttatagg cctggtagat actgaaagct aactgactac
    29341 cgaacccgta acgattcagt cccatgatca cttgatccat cccgtggttt agccgaatcg
    29401 tctcagcaag catttctctt tttggcttcg tcaaaccgga gatttgtttt aataccgaag
    29461 gatcagcaat gattttttcg atagcatctt ctcccagcaa ttctacaatt ttttctgctg
    29521 ttttcttccc gattcctggg aatttttcgc tggaaagata cgctacgatc ccatttgcag
    29581 ttgttggctt ttcttgctca tatctttcga gtttaagctg ccttccgtat ttaggatgat
    29641 ccactaattc tccataaaaa cgataagtct cattttcttg aatatcgccg aaacttccgg
    29701 tgactacaat ctctttttca cggtaatcag ctgtggtttc actgatgtgt accaaaagca
    29761 ctttgtaaaa attactagga ttttgaaaaa aaatcgcggc aactgtacca acgaaataag
    29821 gcatttcttc tggctgcatc ggaagccgcc tccttcttct atagtaaagg ttctggtgct
    29881 gtttcagttc ctaaaaaatc tgtttggttc catactttca gttcatatgg caataatctg
    29941 caaggctctt cagtctctaa aatcgtcaaa ctactattga acaatccacc catttcacgc
    30001 aactcactta gctcttttcc tgtcatccat tgaatggctg cagttaaaga agctccatgt
    30061 cctacgaata aaagtgggcc atctttatgt tgggcaaccg catctgctac aacagtttcg
    30121 atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag gagaaggatc atacagatct
    30181 aaatgattac gcaaattcgg cagattatcc ggatatttct ggtacatttc atcgatcgac
    30241 tgaccttcta aagtacccag ccctagttct cttagcttat ctgtataaaa aatctctacc
    30301 ggacgagcaa gctctttgtt gataccttct gctgtatcac gtgctcgctt tgctgtactg
    30361 gaataaattg ctttaaaagg aacatatttt acctttttcc caaatgcttt gatttcttcg
    30421 taactttgtg gcaaaagcgg agaatcgcca ttgctccctt gaaaacgtct ttcctgattc
    30481 cattgtgtct tcccatgtcg ggtaaaatat agttccatac gcagcaaact cctgtctttc
    30541 ttttatttta ctctataata aactgtctaa tccagcttgt cattttcata gtgatgcgtc
    30601 aattctaaat tagggaatcc ttgctggcga agtgcttcat acactatcat tgcagctgta
    30661 ttcgataaat tcaatgagcg gacatgctca tcattcatag ggatgcgtaa acacttttct
    30721 tcgttttcac gcataaatgt ttctggcaaa ccggttgttt cttttccaaa taaaaagaag
    30781 tgctcttctc catcagcaaa attttcgtca ctatatactt tatgggcaaa ttttgagatc
    30841 aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt ctaaattact gtggtaagta
    30901 atatttacat cattccaata atccaaccct gcccgtttta gatgtttgtc atcagttgaa
    30961 aaacctagtg gctcgattaa atgaagcgga gtattcgtgg ctgcacacgt acgggcaata
    31021 tttccagtgt ttgcagggat taatggttca aataaaacga tatgattcat caggatcatt
    31081 cctctcatat ttatgttcgt ttctattttc tgacaatttg atttttgcat aaaaaaaaac
    31141 gtatcaactc ggtgtcagtc actgcgagtc gttacgttag tgaagctctt ctcactaact
    31201 tttatcagaa caggaacctg ataaaaacca atgaaaaaac aacttgttgc taatataaca
    31261 ccctcaactt ttttttgcaa cctctactca ttaaaaactg ctagaaaaac gttttctctt
    31321 agttcctttt aataattcta tgtcaattgt aatcttcttc aaaggattct tatataagtc
    31381 tgctttgatt cgctcttcta cctgccattc taaaggtgac atggccaaaa gatctttttg
    31441 attttcctct ggtatctcaa attcataggt tacacgctga cgttcagtta atttcattgc
    31501 ttcagcaaac ttggctacta cacgttcatt ggaataactc tgtttttcag gttgattcgg
    31561 ataaaaagcc tgacgtagtt cctttaaata attttcttgt ggcaccacct tcaacacttc
    31621 tccatcttct gagagtacac gttggaattc ttggtaatga gaaggcgaaa agatatttaa
    31681 aaccgcgttg aatgtttgat cagcaaacgg caaattggtc aaatcagcta cacaccaaaa
    31741 tgcttcggca gattgttctg tagctgcgta gactccttct tttgaaatat caaatccaac
    31801 agcattttga atctttttca attttgaaag ttcattcaag aagctacctt ctccgcagcc
    31861 aacatctaat agcgagactt cttcaggtaa atgctgcgca atagtctcaa taacgggctg
    31921 atacatgcca gattggatca ttctacgcct tggaacgaac attccttgat cgtattctgt
    31981 tttaaccgca tgggataaaa aataaagtgt tccttttttg gaaaggtcaa aacggtgatt
    32041 atttttgcat atcaagccat tatcagactt ttttacttct tcttggcaaa ccggacagcg
    32101 gaacagctga tgattttctg ccaaaaactg gcgtcctcgg tcaatttttt ttaacatagt
    32161 tccccctcat ttctgtttta tcttaccgtt agatgctcac tttggcaact gctaaaaata
    32221 tgatacaatg cctagaagaa agtgaaagat aaaggagaag attagatgat caaagaattt
    32281 tgcgctgaaa actacacatc tattcctcta gcaattgcta gaggagccga tcggatcgaa
    32341 ttgtgtgaca acttagcggt cggtggcaca accccgagta ctggtgtgat cgaagaaacg
    32401 ttaagttatg ctggggaaaa agatatcccc gtaatgacga ttattcgtcc tagagggggc
    32461 gacttcgtct ataacgatat cgaattgaaa atcatgcata ctgatttgat tgaagcaaag
    32521 aaattaggga cggatggcgt agtatttggt tgtcttactc cttcaggctg gctagacgaa
    32581 gaggccttag aactattgat cgaaacagca gaagggttgc agatcacctt ccatatggct
    32641 tttgacagta ttcctgaaga tcggcaattc gaagcaatcg attggctggt agaacatggt
    32701 gttgaccgta tcttgactca tggcggtcct gctgacaaaa ctattgtaga aaatttccca
    32761 cggcttaaag aattgattgc ctatacagat ggccgcttaa tcattttgcc tggtggtggt
    32821 gtcactgcag agaatgcagc acatgtagct aaagaattga acgtaagcga agtacacgga
    32881 accaaaatcg tggatcttca gttataatct tataaacaaa aaaacatttg caacaggcat
    32941 aatgcttatt gcaaatgttt ttgttttttt acataaaata gcccataaat aaagaagcaa
    33001 tattgaaata aatcagtaca ctcgtcaaat cgctcaaagt cgtgataaag ggaccgcttg
    33061 ctactgctgg atcaaatcct agacgatcca ttagcatcgg gattaaacta cctgcaagat
    33121 tcgccactgt gattgcaaat agcatagcca tccctatgac aaaccccaag ataaaattat
    33181 gtttccaaaa acctacgatt agaaaaatcg tgatccccgt aacagctcct gtgacaacgc
    33241 cggtcaagac ttcagccaaa acaagtcgaa caaatccatg atctttctca tcagaaatcg
    33301 caagtcgacg aacagccaca gctagcgact gggttccagc attaccggca gttcctgtaa
    33361 tcagcgaaat gaagactgct aaaatactcg cttcacttac caagtcttca tagtgactga
    33421 ttaaagaagc ggtcgacata cctaaaaaca acagagtgat cagccaaggc aaacgtcgag
    33481 acgctgcctt tactggattt tcattaattt cttccacatt gaccccggca agtcctgaat
    33541 aatcgctagc agcttcatca tcaataacgt cgatgatatc atcgactgtg acgattccta
    33601 acaaatgatc atcataatcg gtaacaggaa gagctaagaa gtcataatct ctaaatgttt
    33661 gtgccacatc ttcttggtcg tcgcctacat gaactgaaag cactcgttcg ctcatcagat
    33721 cagaaatcat tgcgtcgtca tcattcacga ttaggtcgcg cagagaaata acccctacta
    33781 gctgaccttc ttgattaacg acatagatgt aatagattgt ttcagctaca tctgcttcgt
    33841 tcttcagcac gtacatcgca gagcgaacag tttgattggc tacgatcgaa acaaattcag
    33901 ttgtcatgat cgcgccagcc gtttcatcct cgtaatggag aagttccttg atttcactgg
    33961 catcgtctgc cgacatcaaa cttaaatatt tagcaatttg ctttttatcc agcgtattaa
    34021 gcaagtcaac agcattatct gtatacatct cagaaagcat atcagctgca tagctaggac
    34081 gcatttcatc taggtattct ttcatatgtt cgttgtcttc ttcaatcaca tcgaacatat
    34141 ccgctaattc tttaggcgac agataggaat agatcagctg cctgttctct tctgtaagag
    34201 attggtagaa ttgcccctgc tcatagatat gcatctctaa aaaactgttt ctgaatgcct
    34261 gaatattatt ttcccttaaa tcttgagtca actgggcaaa tctttcctct aattcttgat
    34321 tttcatccaa accaatctca cctcgcctta ttcttcaaac cgtctcacaa ctgcctccat
    34381 gtctgcggct aaaggctgct tgaactccag caactcttct gtaaatggat ggtaaaaacg
    34441 cagatcataa caatgaaggg cttgtctttt tattcccaga tccattcgcc cgccatacat
    34501 atcatcaccg agcagggcac agccaattgc ggaaaaatga actcttattt gatgtgtcct
    34561 tcctgtatgc agctggatat caactaacgc gatttctgtt agtcgcttct ctatccagta
    34621 ctcagtctgt gccgaacgtc cttcatcaga gaccattcgc ttcaatagcg aagaataatc
    34681 tcgggcgatc ggcaaatcga tatgtccatg tgccgataaa tctgaaagcc ttccactgac
    34741 taaggcctga tattttttca caaacttctt ttcacgaagt tgcatatcta attttgcgtg
    34801 cgcaaatcca tgctttgcaa aaagcatcaa accagatgta tcccgatcta gtcgcgtcac
    34861 cacatggatt acttggtttt cgtaaccttg ttttttataa taagccttta cacgattagc
    34921 cattgtccca tttggatggt attgggcagg aatagacgaa acgcctgctg gtttatttac
    34981 taccagaacg tgttcatctt catacacgat atcgatcggt gtttcatcca aaagtacggt
    35041 ttcatgttct ccttcagcag gaatcacaat ggttacctta tcatccttag ctagagaaaa
    35101 caagacattt tctacttgat cattaacttt gatttgaccg ccttgaaatt ttattttcgc
    35161 taacagaccc tttgagatcc ctttttcttt taaaaaatat tttacttgct gagaatgatc
    35221 tttctcataa tcccatgtaa actgcatatc aatcctcact aataaaggca tctttcacac
    35281 gatgccaaaa atgcatatgt cgataagaag caaaatggat ccgttcttct gctattcgat
    35341 aataaattga gcggatattt gcttttgcga cattcaattg atcaaccgtt acaagataat
    35401 cttctgtatt ttccaatttt atttctagcc attcatcatg ggcaatcacg atcggagaac
    35461 ccaaagtacg aaacactcga ttattcagtg aagcaatctc tgccaactga aaggcattga
    35521 tactaggatg gatcacagca ccgccgacac ttttattata tgctgtcgaa ccagtaggcg
    35581 tagaaatcgt caatccatct cctctaaaac tttcaaacag ctcgtttttg atgtatacat
    35641 cggctaccat cgtacgattg gcccgtttaa tcgttgattc attcagtgct aaaaaatgct
    35701 ggtccggtgt ttcatccaaa taactgatcc gaacatccag caagggataa ctaacacttt
    35761 gttcccggtt cgttcgtaag ctgtctacta gttcttccag ctcgtaatcc cgccaatccg
    35821 tgtaaaaccc taaatgacct gtatgcacgc ccaagaaacg cacatcattc agacaatgac
    35881 tgtagtgatg gaacgccgac aataatgtgc cgtctccgcc gactgagatg accaattctg
    35941 gctcatgatc atctcgttga atccctgctt gttctaaaag tgcgttcaaa cgttctgtca
    36001 cttctatagt ctttgcttct ttattatgaa cgatcgctac tttcatcccc tgcctcctct
    36061 ctttcgcttt ctttcttcaa aagcgttcga tagtagacat catctgattt tcctcttcca
    36121 tgggagaata accgctgtgc ttcttggatt tcttcacgga tactggacat ctcttcatcc
    36181 aactgatagg cagcttctgc cgcacgctga agtcgttcac tcatttcttc tggaaaaacg
    36241 ccttgatatt tataattcag cgaatgctca atcgttgccc agaaattcat cgcaagagtc
    36301 cgaatctgga tttccgccaa aattttcttc tcaccgctga tcagctgtac aggatactcg
    36361 atcaccagat gataagaacg gtagccactt gcttttttat ttgtgatgta atctcgttct
    36421 tggagaactt tcatgtcttt tcttttacgg atgatctcaa caacttgatg gatatcttct
    36481 acaaactgac acatgatcct taacccggca atatcagaca tttcttcttc taaacgtccc
    36541 attggaatat gccgtaattg tgattttgtt acaatactat cgactggctt gactctccca
    36601 gtgacaaatt caatcgggac atgtcgattt tgttcacgga actgtttgcg gatcccccgc
    36661 agtttcactt tcaattctga tacagtttgt tcatatggtg ctaaaaatgc ttcccaatct
    36721 cgttccatag ctgatacctc tacttcatta acattcattg tttattctac catatatgca
    36781 aagttttttc gtactcttga tttatgcatt gaccaaaaaa agaaaattag gcaaaataga
    36841 caaaaaggag tcgatcaaat gacacaaatg cttgaaatcg aatataaatc tatgctgaca
    36901 aagcaagagt acgagatatt catcgctcac tatcaattaa cagataaaga tttccgtgta
    36961 cagactaata tttatttcga taccgttgat gaacagctga agaaacaaaa ttgtggccta
    37021 cggatccgtt tcgttggtca tcaagctgaa tatacattaa aaacacctgc tgaaaaaggg
    37081 cgtctcgaaa caacggatac ctttataaca gaagaagcag aagcatttat cttcgaaaaa
    37141 cgtctgcctc gttcaggcgc agtttttcaa aaactttctg atttgaatat cgatcctgct
    37201 tcattaatcc aaaccggaaa gcttacaaca aaacgggctg aattccctat tgaagaagga
    37261 cttttagcta ttgatgaaag ctggggagac aatctacacg attttgaact ggaactagag
    37321 gtaggggatg catctgtggg aaaaattgct tttataaatt ttttaaaacg tttttcttta
    37381 ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg aagctaaaaa ttagaaacct
    37441 ttcattttca caaggcactt gagttcattt ttttactttt ttctgttaag ttataggatg
    37501 tgaaagaatc tagtcactag ttgtatagaa tctattttgg gggagattca tatgatcgaa
    37561 atctatttat ttgttaatcc tttaggggaa tactgtttcg aaatggaaca acagttatta
    37621 caatttatcg aagaagagta cgggacacca tcaaaagaaa agatgcaatt tcgctttctt
    37681 cctttggtca atttgcagac aattggggat gtcatgcaac aaaaaggaat ttctcagaac
    37741 aatcttgagg aacgtaatca cttattctca atgacttatt ctgcagcatt ggactgcaaa
    37801 gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt tgatcaaatt gcaagaagca
    37861 gtcggctgta atgacatacc atattctcaa gaacttgctg aatccatatt tgaagaaatc
    37921 ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg acttcgttaa agaagtattc
    37981 ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa aacacccttc tgcagtcgtt
    38041 tacaattatg cctgcgaccg tgattttggt gttttagtag aagtaaaagg tacaagtagc
    38101 ctttggagaa tcacagagct gtgtaaaaca acagataact catatcaagt ttttaatggt
    38161 gaacatttac atacaaatga ttctcagttc cattcaagaa ctagccattt acacttgctt
    38221 tctaattaaa aaaatacgtc gactccctta caagagagcc gacgtatttt tttcgtttct
    38281 attcgagaaa atcaaaaata gaaagcctta taaacgatcg atcaattctt ctaattcatt
    38341 tagacgttgt tcaaacatcg acattgcatc ttcaatatat gcagcctgtg tcatatcaac
    38401 tcctgctttt ttcattactt ccacaggata atcgctgtta cctgctttta ggtaagccaa
    38461 gtagttctct agtgcctctg gttcctggtt tagtattttc ttcgccagtg ctgaagcagc
    38521 agaaaagcca gtcgagtatt ggaaaacata gtaattataa taaaaatgcg gtatccgtga
    38581 ccattcgaat ttaatttctg ggtcttcttc gactgctgga ccataatatt ttgcattcaa
    38641 tttaccataa ctatcactta ggtattcact agtcaatggc acaccttttt catcttccgt
    38701 atgcatgaaa tgctcgaatt cagcgaattg tgtctggcgg aaaactgttc ctttaaaccc
    38761 atccaagtaa tggttgagta cataagcccg tacacgaggg tctttttctg tttccaataa
    38821 atactccgtt aggatatttt cattcgttgt cgaagcaatt tctgctaaaa agatggagta
    38881 gtcgccgtac acataaggct ggttcgaacg agtgaaataa ctatgaacac tatgtcccat
    38941 ttcgtggaca agcgtaaata gctgatccag cgtatcatgc caattcaata aaatatatgg
    39001 atttgtgtca tagcttcccg aagaataagc accgcttcgt ttccctttat tttcgacaac
    39061 atcgatccaa cgttcagaga atgctttttc tacgatggcc atgtattctt cacccattgg
    39121 tttcagtgct tctaaagctt tttcttttgc ttcttcgtac gtaaaggtaa ttggagcttc
    39181 ccctaagacc ggtgtataaa gatcatacat gtgcagtttt tccacttcta ataaacgttt
    39241 ccgtaattcc atgtatcgat gtaacaaagg caaatgcttg tttaccacgt ctaccaaagt
    39301 atcgtataca ctttcaggaa tatgattatt gctcaaagac gcttctctgg cagagctgta
    39361 attacggact ttcgctttaa aattatgtcc ttttatatgt gtgcctaaag tagaagcaaa
    39421 tgtatttcta aattgttcgt aaacactgta caatccctta aatgctgctt cgcgcaccct
    39481 tcgatccgtg ctttctagca actgaccata cacgccatga gataattgga ctatttcacc
    39541 attttctcct tcaatcgttg gaaaaactag atctgcatta ttcaaaacag caaatgtatc
    39601 acttgaagca tcaaagattt cacctgctcc agcaagaaga gattcctgct cagcagataa
    39661 gacatgggct cgattatcta ctatttgctg gatataatgg cgatagactt ccaattttgg
    39721 ttcttctttg aaatattgcc aaatctggtc atctgacaat tgcaatattt ctggttcaaa
    39781 ccaggaaacg gcttcactaa ctttcgaaaa cagactgctt gctcttgcat aaagcgcttg
    39841 gtaatctgta tttccagtgt cttgatcgtt tttaaggtgc gcatatacat aaatgacttc
    39901 agtttggcga taaaccctca atacgaattc aatcgcattt aaaaattgag aagcgccttg
    39961 atcaagtgtt cctttgtgtt tttcagattg ttttaactct tctgataatt ccaaatattt
    40021 ctcatcaaac tcttggtcgc ttgaaaagat cttggtcaag tcccaagtta aattttcagg
    40081 caattcttct cgttttggca actgctttac ttccatgaaa tccactcctt ttcttccctt
    40141 agctctatct atcaaaatag atcatagtct aacatccaca tgtcacagca tctaactttt
    40201 ctactcattc atgtgtctgc ttccatcaat tttagcataa ctaaatcttg ttttacatag
    40261 aaaaccgatt cctagcctat ttttatactt actatttttt caaagaagac aagtgtatgc
    40321 attcgttaaa aaactgctga tagaccatct cttcatcaat cataggaaaa agccattccc
    40381 ttttatgttc ttttatttgg caaagccatg agttgaaatc aggaactttc tggttttcca
    40441 aggcttcttc gtacaacatt cgataaagaa aaacctgttc ttgtaaataa aagaaaaacc
    40501 gacttttttg atagatccac gatgatagat gcaaaagatg tttatgacgc aagtagcatt
    40561 gttcttgtat ggatactgta ttcttttgct tgtgaataag ttggcgtgtc agccatttct
    40621 tcccatcttc actcactttt tctatttttt tcatttggtt cattttaatt tcgttactag
    40681 tgaatatttc tttaaggtcc ctactagaaa aagtccagca gctactgcaa aaggaaatat
    40741 gccctgatac agtctctgta atatgatgat acaaataaag cttttgtttc ttgagatcag
    40801 ctttccagca atgcactcct ctttttctat tatagtagca gcaacttttt tctatcaatg
    40861 agaattgcga ttgtccaaga aaagaattac caagtatcca ccatacttgg taactgttat
    40921 tcaaataatt ttgggttctt tcaataaatc tctggtgaga cagtctagaa cattggattt
    40981 ctattgctag attcgccaac aaaatatccg gcctttgccg caacctcggc aaataggctt
    41041 ccaaaaatac tggttctact gactgtccca gccaatccat gaagcattcc tttaactgta
    41101 agtgctcttt tgattctctt tcactaaaga ccgaacagtt atctgatttt tgatgggcaa
    41161 aatgagcaca ttttttcttt ccctttttca aaatcactct ttttcgacag ccacgacaat
    41221 aatatttctt tcctttttct gcatgtttag caaaacagca cttattatat tcattcattg
    41281 caagcagcat ttttctccct ctgaacttat ttacgtctaa gaatttattt acacgaaaaa
    41341 agagggcgtg aaaaagctat tctgcatcaa gtaataagaa cagctaaaca aaaatagttc
    41401 ccaatatttt tcgtttggtt gggcttgtta ccgcagatgc aagcttttga acacatttat
    41461 acgaaaaaaa gactgcgaca caatttttgt cacagtctct tactataatg ctgatttata
    41521 caaaataaaa tttcgttcgc tcgatagcat tacgcgccat cagacattgt ccatattcat
    41581 tcaatgtttc tggtgtacga tcgctctttt cagcaaactc caaaattcga gcaatctgat
    41641 tctctacatc cgcttggttg agattttcca tccagaagtg aacgactagg taatagcgct
    41701 cttcgaataa atacaaatca gtccaagcag actctagttc gacatctgtt gcaagttgga
    41761 tcacttgttc aaaatcttca aaagcaaaag ttgcttgtct ttcctgttct tctagggcgc
    41821 ttccagataa ttcttcctcc ggatcgctgc caataatctg ctgacgaaga aagtcactca
    41881 catcttctga atcaacagta gaatcttctg attgagaaat ttcttcatcc ggaagatttt
    41941 tactgataaa cagttctaat ccgtctcctt ttggcaaaac ttggaaagtg acagcttcgc
    42001 ttcctcggaa ctcctcttcg atatcaactt cttccaaaat actataaaag aaattttcga
    42061 tttctttgtg attgccaagt aaatccaaaa atgaaacgcc ccgttcctct aaatcttcat
    42121 gaccaattag tacacggatc gtattttcat taatatgttc catttccatt ttgctacacc
    42181 tcactttgcc acaatgttta atatagattc attgtagcga aatcagctta aatgtaaaga
    42241 aaaaaaatct cgctgttaag cgaatttaat aaaaccagcg gatttttcaa cctttcaagc
    42301 tatcttattt aactataaca tttttggcag acgaatacta tatagccgac ttttcagcta
    42361 attttccctc tctatttttc gtgcaatcat catcttctac taccttttta tacatctaat
    42421 caagaatact ttttgtaaaa acaaaaaaac ttggaaaaac tatctttgta atagt
//
LOCUS       DAGCXG010000017        39341 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGCXG010000017 DAGCXG010000000
VERSION     DAGCXG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.017"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            179..3457
                     /locus_tag="MB022_000902"
     CDS             179..3457
                     /locus_tag="MB022_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBT4465916.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3998..5905)
                     /locus_tag="MB022_000903"
     CDS             complement(3998..5905)
                     /locus_tag="MB022_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBT4465917.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6035..6910
                     /locus_tag="MB022_000904"
     CDS             6035..6910
                     /locus_tag="MB022_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBT4465918.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6990..7964
                     /locus_tag="MB022_000905"
     CDS             6990..7964
                     /locus_tag="MB022_000905"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4465919.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8144..9289
                     /locus_tag="MB022_000906"
     CDS             8144..9289
                     /locus_tag="MB022_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4465920.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9426..9770
                     /locus_tag="MB022_000907"
     CDS             9426..9770
                     /locus_tag="MB022_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBT4465921.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10041..11327
                     /gene="efmA"
                     /locus_tag="MB022_000908"
     CDS             10041..11327
                     /gene="efmA"
                     /locus_tag="MB022_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBT4465922.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11465..12064)
                     /locus_tag="MB022_000909"
     CDS             complement(11465..12064)
                     /locus_tag="MB022_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBT4465923.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12468..13589
                     /gene="mnmA"
                     /locus_tag="MB022_000910"
     CDS             12468..13589
                     /gene="mnmA"
                     /locus_tag="MB022_000910"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBT4465924.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13835..15256
                     /gene="arcD"
                     /locus_tag="MB022_000911"
     CDS             13835..15256
                     /gene="arcD"
                     /locus_tag="MB022_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBT4465925.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15270..16388
                     /locus_tag="MB022_000912"
     CDS             15270..16388
                     /locus_tag="MB022_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBT4465926.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16448..17740)
                     /locus_tag="MB022_000913"
     CDS             complement(16448..17740)
                     /locus_tag="MB022_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4465927.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17872..18450
                     /locus_tag="MB022_000914"
     CDS             17872..18450
                     /locus_tag="MB022_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4465928.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18579..18956
                     /locus_tag="MB022_000915"
     CDS             18579..18956
                     /locus_tag="MB022_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBT4465929.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18889..20961
                     /locus_tag="MB022_000916"
     CDS             18889..20961
                     /locus_tag="MB022_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4465930.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21172..22119)
                     /locus_tag="MB022_000917"
     CDS             complement(21172..22119)
                     /locus_tag="MB022_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4465931.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22365..22829
                     /locus_tag="MB022_000918"
     CDS             22365..22829
                     /locus_tag="MB022_000918"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBT4465932.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22891..23934)
                     /locus_tag="MB022_000919"
     CDS             complement(22891..23934)
                     /locus_tag="MB022_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBT4465933.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24311..25276)
                     /locus_tag="MB022_000920"
     CDS             complement(24311..25276)
                     /locus_tag="MB022_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBT4465934.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25510..26355
                     /locus_tag="MB022_000921"
     CDS             25510..26355
                     /locus_tag="MB022_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4465935.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26546..27733
                     /locus_tag="MB022_000922"
     CDS             26546..27733
                     /locus_tag="MB022_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4465936.1"
                     /translation="MEKKLKTSNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27739..28095
                     /locus_tag="MB022_000923"
     CDS             27739..28095
                     /locus_tag="MB022_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBT4465937.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28097..28435
                     /locus_tag="MB022_000924"
     CDS             28097..28435
                     /locus_tag="MB022_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBT4465938.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28511..28767
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28830..29864
                     /locus_tag="MB022_000925"
     CDS             28830..29864
                     /locus_tag="MB022_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4465939.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29857..30543
                     /locus_tag="MB022_000926"
     CDS             29857..30543
                     /locus_tag="MB022_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465940.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30567..31415
                     /locus_tag="MB022_000927"
     CDS             30567..31415
                     /locus_tag="MB022_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4465941.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31612..32385
                     /gene="sufC"
                     /locus_tag="MB022_000928"
     CDS             31612..32385
                     /gene="sufC"
                     /locus_tag="MB022_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBT4465942.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32398..33684
                     /gene="sufD"
                     /locus_tag="MB022_000929"
     CDS             32398..33684
                     /gene="sufD"
                     /locus_tag="MB022_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBT4465943.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33681..34916
                     /locus_tag="MB022_000930"
     CDS             33681..34916
                     /locus_tag="MB022_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4465944.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34903..35373
                     /locus_tag="MB022_000931"
     CDS             34903..35373
                     /locus_tag="MB022_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBT4465945.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35378..36769
                     /gene="sufB"
                     /locus_tag="MB022_000932"
     CDS             35378..36769
                     /gene="sufB"
                     /locus_tag="MB022_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBT4465946.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36864..38006)
                     /locus_tag="MB022_000933"
     CDS             complement(36864..38006)
                     /locus_tag="MB022_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4465947.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(38045..38779)
                     /locus_tag="MB022_000934"
     CDS             complement(38045..38779)
                     /locus_tag="MB022_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4465948.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            39011..39286
                     /locus_tag="MB022_000935"
     CDS             39011..39286
                     /locus_tag="MB022_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4465949.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT        12771 a         7209 c         8277 g        11084 t
ORIGIN      
        1 tattttaaaa aaacttattc tttttacgtt aataaatctc tttattatca acaacttaag
       61 ttatattttt tctttttata aaatacactt attataattt tgttttttat tgacagtaaa
      121 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
      181 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      241 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      301 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      361 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      421 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      481 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      541 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      601 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      661 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      721 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      781 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      841 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      901 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      961 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
     1021 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
     1081 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1141 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1201 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1261 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1321 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1381 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1441 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1501 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1561 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1621 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1681 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1741 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1801 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1861 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1921 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1981 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     2041 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     2101 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2161 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2221 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2281 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2341 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2401 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2461 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2521 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2581 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2641 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2701 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2761 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2821 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2881 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2941 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     3001 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     3061 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3121 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3181 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3241 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3301 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3361 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3421 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3481 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3541 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3601 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3661 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3721 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3781 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3841 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3901 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3961 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     4021 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     4081 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4141 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4201 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4261 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4321 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4381 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4441 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4501 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4561 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4621 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4681 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4741 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4801 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4861 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4921 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4981 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     5041 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     5101 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5161 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5221 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5281 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5341 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5401 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5461 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5521 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5581 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5641 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5701 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5761 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5821 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5881 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5941 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     6001 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     6061 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6121 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6181 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6241 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6301 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6361 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6421 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6481 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6541 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6601 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6661 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6721 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6781 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6841 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6901 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6961 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     7021 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     7081 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7141 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7201 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7261 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7321 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7381 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7441 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7501 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7561 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7621 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7681 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7741 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7801 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7861 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7921 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7981 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     8041 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     8101 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8161 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8221 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8281 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8341 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8401 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8461 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8521 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8581 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8641 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8701 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8761 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8821 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8881 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8941 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     9001 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     9061 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9121 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9181 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9241 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9301 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9361 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9421 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9481 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9541 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9601 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9661 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9721 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9781 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9841 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9901 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9961 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
    10021 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
    10081 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10141 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10201 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10261 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10321 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10381 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10441 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10501 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10561 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10621 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10681 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10741 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10801 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10861 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10921 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10981 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    11041 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    11101 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11161 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11221 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11281 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
    11341 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11401 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11461 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11521 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
    11581 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11641 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11701 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11761 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11821 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11881 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11941 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    12001 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
    12061 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12121 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
    12181 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12241 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12301 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12361 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12421 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12481 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12541 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12601 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12661 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12721 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12781 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12841 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12901 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12961 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    13021 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
    13081 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13141 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13201 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13261 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13321 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13381 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13441 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13501 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13561 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13621 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13681 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13741 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
    13801 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
    13861 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13921 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13981 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
    14041 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    14101 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14161 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
    14221 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14281 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14341 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14401 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14461 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14521 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14581 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14641 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14701 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14761 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14821 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14881 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14941 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    15001 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
    15061 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15121 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15181 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15241 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15301 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15361 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
    15421 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15481 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15541 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15601 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15661 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15721 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15781 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15841 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15901 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15961 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    16021 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    16081 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16141 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16201 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16261 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16321 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16381 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16441 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
    16501 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16561 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
    16621 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
    16681 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
    16741 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
    16801 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16861 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16921 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
    16981 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    17041 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    17101 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17161 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17221 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17281 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17341 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17401 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17461 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
    17521 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17581 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17641 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17701 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17761 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
    17821 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17881 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17941 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    18001 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    18061 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    18121 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18181 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    18241 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    18301 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    18361 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    18421 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    18481 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18541 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18601 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    18661 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18721 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18781 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    18841 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    18901 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18961 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    19021 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    19081 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19141 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19201 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19261 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19321 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19381 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19441 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19501 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19561 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19621 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19681 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19741 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19801 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19861 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19921 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19981 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    20041 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    20101 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20161 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    20221 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    20281 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20341 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggattgcaa
    20401 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20461 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20521 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20581 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20641 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20701 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20761 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20821 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20881 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20941 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    21001 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    21061 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    21121 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    21181 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    21241 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    21301 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    21361 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    21421 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    21481 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    21541 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    21601 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    21661 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    21721 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    21781 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    21841 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    21901 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    21961 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    22021 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    22081 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    22141 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    22201 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    22261 tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag
    22321 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    22381 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    22441 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    22501 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    22561 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    22621 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    22681 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    22741 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    22801 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    22861 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    22921 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    22981 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    23041 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    23101 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    23161 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    23221 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    23281 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    23341 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    23401 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    23461 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    23521 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    23581 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    23641 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    23701 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    23761 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    23821 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    23881 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    23941 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    24001 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    24061 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    24121 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    24181 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    24241 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    24301 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    24361 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    24421 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    24481 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    24541 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    24601 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    24661 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    24721 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    24781 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    24841 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    24901 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    24961 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    25021 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    25081 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    25141 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    25201 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    25261 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    25321 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    25381 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    25441 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    25501 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    25561 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    25621 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    25681 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    25741 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    25801 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    25861 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    25921 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    25981 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    26041 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    26101 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    26161 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    26221 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    26281 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    26341 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    26401 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    26461 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    26521 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaaacttcga acgataatcg
    26581 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    26641 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    26701 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    26761 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    26821 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    26881 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    26941 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    27001 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    27061 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    27121 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    27181 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    27241 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    27301 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    27361 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    27421 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    27481 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    27541 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    27601 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    27661 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    27721 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    27781 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    27841 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    27901 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    27961 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    28021 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    28081 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    28141 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    28201 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    28261 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    28321 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    28381 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    28441 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    28501 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    28561 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    28621 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    28681 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    28741 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    28801 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    28861 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    28921 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    28981 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    29041 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    29101 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    29161 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    29221 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    29281 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    29341 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    29401 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    29461 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    29521 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    29581 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    29641 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    29701 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    29761 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    29821 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    29881 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    29941 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    30001 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    30061 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    30121 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    30181 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    30241 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    30301 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    30361 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    30421 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    30481 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    30541 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    30601 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    30661 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    30721 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    30781 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    30841 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    30901 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    30961 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    31021 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    31081 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    31141 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    31201 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    31261 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    31321 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    31381 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    31441 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    31501 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    31561 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    31621 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    31681 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    31741 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    31801 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    31861 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    31921 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    31981 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    32041 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    32101 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    32161 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    32221 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    32281 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    32341 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    32401 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    32461 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    32521 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    32581 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    32641 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    32701 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    32761 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    32821 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    32881 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    32941 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    33001 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    33061 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    33121 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    33181 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    33241 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    33301 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    33361 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    33421 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    33481 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    33541 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    33601 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    33661 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    33721 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    33781 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    33841 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    33901 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    33961 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    34021 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    34081 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    34141 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    34201 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    34261 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    34321 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    34381 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    34441 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    34501 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    34561 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    34621 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    34681 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    34741 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    34801 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    34861 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    34921 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    34981 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    35041 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    35101 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    35161 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    35221 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    35281 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    35341 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    35401 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    35461 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    35521 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    35581 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    35641 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    35701 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    35761 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    35821 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    35881 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    35941 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    36001 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    36061 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    36121 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    36181 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    36241 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    36301 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    36361 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    36421 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    36481 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    36541 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    36601 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    36661 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    36721 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    36781 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    36841 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    36901 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    36961 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    37021 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    37081 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    37141 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    37201 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    37261 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    37321 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    37381 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    37441 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    37501 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    37561 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    37621 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    37681 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    37741 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    37801 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    37861 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    37921 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    37981 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    38041 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    38101 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    38161 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    38221 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    38281 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    38341 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    38401 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    38461 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    38521 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    38581 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    38641 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    38701 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    38761 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    38821 gtgttgtaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    38881 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    38941 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    39001 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    39061 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    39121 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    39181 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    39241 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    39301 ctaaaacata tagtaataaa gagtttgttt ttattatctg t
//
LOCUS       DAGCXG010000018        36428 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGCXG010000018 DAGCXG010000000
VERSION     DAGCXG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.019"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            310..630
                     /locus_tag="MB022_000936"
     CDS             310..630
                     /locus_tag="MB022_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBT4465950.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            784..1689
                     /locus_tag="MB022_000937"
     CDS             784..1689
                     /locus_tag="MB022_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4465951.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1686..2459
                     /locus_tag="MB022_000938"
     CDS             1686..2459
                     /locus_tag="MB022_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4465952.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2640..4064)
                     /locus_tag="MB022_000939"
     CDS             complement(2640..4064)
                     /locus_tag="MB022_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4465953.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4275..4721
                     /gene="nrdI"
                     /locus_tag="MB022_000940"
     CDS             4275..4721
                     /gene="nrdI"
                     /locus_tag="MB022_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBT4465954.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4786..6768)
                     /locus_tag="MB022_000941"
     CDS             complement(4786..6768)
                     /locus_tag="MB022_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4465955.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6782..7678)
                     /gene="murQ"
                     /locus_tag="MB022_000942"
     CDS             complement(6782..7678)
                     /gene="murQ"
                     /locus_tag="MB022_000942"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBT4465956.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7681..8742)
                     /locus_tag="MB022_000943"
     CDS             complement(7681..8742)
                     /locus_tag="MB022_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4465957.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8953..9519)
                     /locus_tag="MB022_000944"
     CDS             complement(8953..9519)
                     /locus_tag="MB022_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4465958.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9547..11049)
                     /gene="eat(A)"
                     /locus_tag="MB022_000945"
     CDS             complement(9547..11049)
                     /gene="eat(A)"
                     /locus_tag="MB022_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4465959.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11307..12428)
                     /locus_tag="MB022_000946"
     CDS             complement(11307..12428)
                     /locus_tag="MB022_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4465960.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12554..13495
                     /locus_tag="MB022_000947"
     CDS             12554..13495
                     /locus_tag="MB022_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4465961.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13645..14541)
                     /locus_tag="MB022_000948"
     CDS             complement(13645..14541)
                     /locus_tag="MB022_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4465962.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14810..16150
                     /locus_tag="MB022_000949"
     CDS             14810..16150
                     /locus_tag="MB022_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4465963.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16368..16811
                     /locus_tag="MB022_000950"
     CDS             16368..16811
                     /locus_tag="MB022_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4465964.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17315..18733)
                     /gene="nhaC"
                     /locus_tag="MB022_000951"
     CDS             complement(17315..18733)
                     /gene="nhaC"
                     /locus_tag="MB022_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4465965.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19136..20359)
                     /locus_tag="MB022_000952"
     CDS             complement(19136..20359)
                     /locus_tag="MB022_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBT4465966.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20390..21229)
                     /locus_tag="MB022_000953"
     CDS             complement(20390..21229)
                     /locus_tag="MB022_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4465967.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21246..21926)
                     /locus_tag="MB022_000954"
     CDS             complement(21246..21926)
                     /locus_tag="MB022_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4465968.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21928..23001)
                     /locus_tag="MB022_000955"
     CDS             complement(21928..23001)
                     /locus_tag="MB022_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4465969.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23057..23295)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23338..23571)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23648..24319)
                     /locus_tag="MB022_000956"
     CDS             complement(23648..24319)
                     /locus_tag="MB022_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4465970.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24406..27084)
                     /gene="mgtA"
                     /locus_tag="MB022_000957"
     CDS             complement(24406..27084)
                     /gene="mgtA"
                     /locus_tag="MB022_000957"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBT4465971.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27743..28180
                     /gene="ndk"
                     /locus_tag="MB022_000958"
     CDS             27743..28180
                     /gene="ndk"
                     /locus_tag="MB022_000958"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBT4465972.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28202..29068)
                     /locus_tag="MB022_000959"
     CDS             complement(28202..29068)
                     /locus_tag="MB022_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4465973.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29228..31321)
                     /locus_tag="MB022_000960"
                     /pseudo
     CDS             complement(29228..31321)
                     /locus_tag="MB022_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            complement(32070..32693)
                     /locus_tag="MB022_000961"
     CDS             complement(32070..32693)
                     /locus_tag="MB022_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465974.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32852..34249)
                     /locus_tag="MB022_000962"
     CDS             complement(32852..34249)
                     /locus_tag="MB022_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4465975.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34281..35252)
                     /locus_tag="MB022_000963"
     CDS             complement(34281..35252)
                     /locus_tag="MB022_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4465976.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35626..36051
                     /locus_tag="MB022_000964"
     CDS             35626..36051
                     /locus_tag="MB022_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465977.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10529 a         7600 c         6388 g        11911 t
ORIGIN      
        1 attgcccgat aatatataga aataaggtct agttactgtt ttttttaaat gattaataaa
       61 taacaacttc tttctatgaa attgcaaagc attcatcttc ttttccgcta acgatatatc
      121 cgtcagtgct tggtacactt tactaaaaag tatgagattg ccgatatctg gataggtgtg
      181 ctatactaca gaaaaagata tacaatctgc caccgggtag gataaagagg atgatcattc
      241 cattaggtct ttgaagggat gattttcctc tttttgtgta tttttttata gttaaggagg
      301 aatgtaagta tggcatggat ggaattaatc atagcaggaa ttttggaagt attctggtca
      361 acgatgatga aatggagtga tggtttttct aaaatcaatt attcttttta cacagtgatt
      421 ggcatgattg ctagttttta ctttttgtca aaggcaatca aaagtttgcc gatgagtctc
      481 tcttatccta tctggacagg gattggcgca gttggttcgg tcataattgg tgtggtcttg
      541 tttcatgata agctgaatct ttccacttgg ttctttgtgg ggatgctact catcagtatc
      601 attggcatca agattactag cggccactag acacttggaa taagtactga tcagaaaaaa
      661 taagcctttt tatttaatat tcaatttttt tatgaaaaaa taagaaataa gtccaatgac
      721 ctagcgactt ctccactcat caggtgtatg cttgtactat ctatagaaaa ggagtgaata
      781 tttttgaaaa gtctttcggt ccaagaatta tccaagaact ttggtcacaa acaagccctc
      841 agcgatgtca gttttagctt atcccctggc aaaatcgttg gattagtagg tcctaatggc
      901 gctggaaaaa caacgatcat gaaagctatt ttaggactga tccattactc agatggaaca
      961 atctgtattg gtgaaaatga gatttctccc tctagtcata aagggttgga acaagtagga
     1021 gcattgatcg aatacccggg tatctatcca ttcttaacag gctatgatca tctgaagtta
     1081 ttctctgaaa ccaacgatgt atctgctatc gacactatcg tgaatcagct caagatggaa
     1141 aagtacattc acaaaaaagc gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt
     1201 gctcttgcct tactgaacca tccgtctatc gttattttag atgagccgat gaacggtttg
     1261 gatccacaag caacaagaga tgtgcgaaaa atcattaccg acttggctac tcagggcacc
     1321 tctttcttga tctccagtca cttactcagc gagcttgaaa aaatggtcga tgagctgatc
     1381 ctgatcaatc aaggaaagat catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg
     1441 caagatttca tcgttatctc tacaacaaat gatctagctg ctcgaacgat cttgacagaa
     1501 gctggatata ctttggaaga tcctgataaa atcaagcttt taaaagaaaa cgaagaatta
     1561 ttaggaaccg tgatccagct attgtctgca caaaatatcc atgtgacaga cgtccagcat
     1621 ctgacgaatg atttggaatc ttctctattg gaaattctgg attcagtaaa gggggaagaa
     1681 gaaaaatgac attgcttgta aaacaagaat tatttaaatt gatcaagaaa aagtcaactg
     1741 ctgttttatc tgttctatta gttgttttgc tcataggaac agcacttcta gccaaaaaat
     1801 acacgacgat cattgatcca gttgaaatga ctgctcagct ttttagtgcc acatcttgga
     1861 ttgtctttat catgattgca gctgcaagca cgatcatttc gatggaagca caatatggta
     1921 cattgaaaaa tctcctttat cgaaagtatt ctcgaggaga aatattagta agcaaatgga
     1981 tcacattagt tatttattct gtttatttat accttttagc tattattgtc actgtgttga
     2041 tgaaactgat tcttttccct tcgatttcct tcacggagaa ggtttctact ggtcaaacac
     2101 tgatccaaag cttgttcact tatactttgg gtagttatat cggtttatgg ctgattttaa
     2161 gtttagtgtt gatgattgct tgctttatca atagttccgg cgcatccatc tcagcaggaa
     2221 tcgtctttta ttttgcttcg tcaatcatat ctggtatcct tattgcactc atccaaaaat
     2281 gggagtggat caaatggaat ccgatcagta tgttgaatct gcaaaaccaa attggaaacg
     2341 aagagatcat gaagcaattg actcatcttt ctactaatca aatgctattc ggtaatttag
     2401 cttacatcgt tttgttctta gcattaggat atctcgtatt taagcgaaaa aatgtatgat
     2461 gactcatcaa aaaagaggct gtgaaagaga cgttcagcct tattgccgat tggctgtctc
     2521 ttaaacaccg tttattctga aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc
     2581 tagtatgttt ttgaataaac gacaggctca caacctctct ttatacttct tttatacttc
     2641 tatacgtgaa gcatcccagt ggcaaataaa accattgcaa tcactccgac catcgtcaca
     2701 cctataagta aaatcttatc taagtggata tgttgtacag gctcgttttg ctccttacgt
     2761 gctttgaggt aaaaataaat gcctggcaag aaaagaacaa gaacaagcat tacttcatta
     2821 aaggctacga ctagcatagc ccatacttga aaggcacttg ctaaaagccc tactaaaaat
     2881 tggacccatt ctttccgctg ataagaaagt ttcatttggt acaatcctaa gaaaagccac
     2941 gtgaagaaaa tagctgctgt accaagtgtg taagcaaagt tataagcact gtcagtgaat
     3001 aataatgaaa gtaaaaagac attcgtacat aacgtcgtca atacgatagc ataagttggt
     3061 gccccatttt tgttgagttt tccccatgct ttaggcaaaa gtccgtcttc agccatgttt
     3121 cccattgttt caatcggtaa gatcgtccat gagagccaaa caccaactaa agaaatgatc
     3181 aaaccgccgt tgatcacgac tgctccccat ttaccaacca tcatttcaaa cacatagccc
     3241 atagcaggct gcgccttgat cgtttgcagc tgattcatcg tcattgtccc atatggaagc
     3301 atggaaacca agacgtaaat aataatcaag ccgaatatcc ctagaatcgt tgctttccca
     3361 acatctgatt gtttttttgc tcgtgaacct acgacagagg ctccttcaat accaacaaat
     3421 acccaaagca tcaccatgat gcaactcaat acttgactgg aaaacgggat cggctgtcca
     3481 ttcgcattca atataatgtc gctagaaaag ttatcaatga attgttggat cgaaaacgtg
     3541 ctgatagcta ctacaataaa taaaaataat ggcactaatt tacagaatgt gacgatagca
     3601 ttgacgaaac tagcattttc gactccccga ttcactagcc atgcatatcc ccataaaaag
     3661 actgtactca tcacgatcga tgccagattt tgcccacctt caaatactgg gaagaaatat
     3721 cctaatgcgc tcatcaacaa tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag
     3781 taaccccaac cgctgataaa accagcgaaa cggccaaaac cttcttctgc gtaactaaag
     3841 atcccgcctt ttaaatctgg acgtttttta ttgatattat tgatcgacaa ggagagcatc
     3901 aacattgctg ttcctacgag tacccaactg aaaatagctg ggccgccagc cgatccggag
     3961 atgtccgtca taataccaaa tacgccacct ccaatcgctg aaccaattac taaggcagca
     4021 agtggaatta aaccaattcc ttttttctgt tgctctgtat ccatgtaagc atcctccaaa
     4081 ataaaaccgc ttttatcaaa aagtataaca aacctttttt atttttactt aattaataaa
     4141 aactttaata gaaatataat tctaccggct tttttctcat tatacaagca aagcgcctag
     4201 tccctttcca aattacacca ccaaaaaact taaaaaagat gctatactaa aaggaaagaa
     4261 ttggaggatg atccatgaac attttatata tatcgatttc aggaaatacg cgtgcctttg
     4321 ccaaacatct agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag
     4381 ttactttaaa agaaatccat gaaaatagtg attttacgaa agaaacagaa ccatttttta
     4441 ctttcgttcc tacttattta gacggcggaa atggtttaga taacggtgat acagaaatct
     4501 tgaccgaaac gatgcgtgaa tatctggaac atgaagacaa tcataaactt tgtcttggtg
     4561 tagtcggtag cggaaataaa aacttcaata atcagtattg tctaacagcc aaacaatatg
     4621 cacaaacatt tggctttcct ttcttggcag attatgaatt acggggaaca ccatctgatg
     4681 tgacacgaat ttatcagatt ttagcagaaa acaataaata ataaaaaaga agaagctgaa
     4741 aaatagattt cagtttcttc tttcgtttct attgtttcta tcatattaag ctttgacatc
     4801 catcacaggt gcttttgctt ttgctttacc tgttttttcc aagacaaagt tttttacacg
     4861 atccatattg gttaccaaga cgatcatgga tgtttctttg cctgctgctt tgatcgcttc
     4921 taagtcagct tctgctacaa gcgtatcagg tgtcacttgc tgcccttctg ttactttcac
     4981 atcaaatggc gtaccgccta agtcaactgt gttgattccc atatgaagca agatttctaa
     5041 accatttggc gttgtgatcc caatagcgtg tttcgttggg aagacagagc tgactacccc
     5101 agtaactgga gaatagatat gtccgttttc tggttctaca aagtagcctt cacccatcat
     5161 cttttgtgca aaaactggat cacttgcttt atctaaagaa acaactgttc cgtcagcaac
     5221 tgatgagagg atagtcacac tttcagctga agtttgaggt actacttttt caggcacttc
     5281 atttactgtg gctgtttcag tcgctaattc ttctttttct aaacggtctt taggaatacc
     5341 gaagaagaat gtagcaacga agccaccagc ataagctgcc aataatccaa ggatgtagcc
     5401 taaccattta ccgtcagaga tcaatgggat caaagcggct cctgaaggtc caatagcgat
     5461 agccccgaca tttccgatca ttccgatcac tgccccgccg atacctccac cgatacaagc
     5521 agtaataaat gggcgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc
     5581 taggatcccg actggtaaag ctcctttgat catttctgtt aattctttat cttttttgca
     5641 gcgaatccat aatgccaatg ctgcacccac ttgtcctgca ccagccatcg caagaattgg
     5701 aagcaatagc gtacgaccag tttcatcaat catttgcaca tggattggag tcaaaatttg
     5761 atgcagtccg aacataacca tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc
     5821 tccaccaacg ccaagtaccc agttgatccc accgaccatg ccatcagaaa tgaaacctgc
     5881 taaaggcatg atgaagaaaa tttcaatcag accgatggca atcaatgaaa gagttggtgt
     5941 aacgatgata tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa
     6001 tagccaaaca gcgaagatga cacctaggat cccaccttgt ccagctgcaa gagcttcacc
     6061 agtaaataag tttttcagtg gtgcttccgg attcatccct gtcagcatca caacggcccc
     6121 gataactcca ccaagagttg gtgttgcacc aaaaacttgt gcagcattga tacctgtata
     6181 gataactagg tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg
     6241 ccatgttgct gcatcaagtg ttccggctgt gactaagttt gctaaaactg cggcaatccc
     6301 tgccacgatc ccagtaccga caaatgcagg aatcattggc acaaagatat tagaaatgtc
     6361 ttttaatacc gatttgattt tagatggttt ttgtttcgat ttttgtgccg ctttcatttc
     6421 ttgtgcccgt tcgtttacac ggtcttttcc agatgctgat gaatttgctg tagctgtact
     6481 tacagaagaa agtttttctc ctaatttcac gcctgccaaa tcaaccattt gctgtgctac
     6541 tttgttcact ttcccaggtc cgatgatgac ttgaagctgt tcatcttcta ctacaccaag
     6601 cactcctgga atcgctttta attcagagac atttacaaga tcatcattga tgatcttcat
     6661 acgcacacga gtcatgcagt ggacaatgct ttctacattg cccatgccgc ctgccccttt
     6721 ataaatttgt tttgccagtt cttccaaagt cagttcgttt tttgccatgg tcttttcccc
     6781 ctcataaggt cccttttaca aatccgtttg cttcagctaa ttttgcttga gcttcctctt
     6841 tatcactatt tgtcaaaatc atgacgatag ccagcttaac attttgatct gcttcttcaa
     6901 agtacttttc agctgtttcg taatcagctt ctgtcgcttg catgatgatt cgttttgagc
     6961 gttcgattag tttttcattt gttggtttga catcgaccat caagttattg aatactttac
     7021 ctataccaat catactgata gtagaaatca tattcaagat aagtttttgt gctgttcctg
     7081 acttcaatcg agtagagcct gtcaaaaatt ctggacctgc atcgacttcg attgggaaat
     7141 ccgcgtgttt actgatttcg gcttgtttat tgcaagaaat cgttcctgtg acagcaccga
     7201 tcgattttgc gtaatctaat ccaccgatca catatggtgt acgaccgcta gctgcgatac
     7261 caataactat atcatttttt gtcaaattca gcttgattag atcttcttgc cctagctcat
     7321 aagaatcttc ggccccttcg acagctacag tcattgcttc ttctccacca gcaattagac
     7381 ctatgaccat ttccggttct acaccgaatg tcggtacgca ttcagcagca tctaacacac
     7441 ctagtcttcc acttgttccg gctcccatat aaatcagacg tccacctttt ttaaaggact
     7501 caatcgtgtt agcaatcact ggttcgatac ttgtcagttc tttttcaacc gcagcggcca
     7561 ctttctgatc ttctttgttc attagttgaa cggcttgaga aatactcatc tcatctaatc
     7621 caaaagtttc ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt
     7681 ttatcttcct tcttttctta taaattgata gttcatacca gctttgattt ggttgatcaa
     7741 tggtagctct tctttgataa ctttagctac tacattcact ttttcatccg ctggcaaatc
     7801 atatttggtt aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat
     7861 agaccctttc atacgcgtag ctgtatttct tgctgggata ggcggaatct ctcgaaaacg
     7921 tgcatctacg cttcgtatga catctcttgc atcgtcttgc cgattcgtat gcttccctaa
     7981 tatatattcg taaaattctt catctacagg ttccgtatgg agcaagactg tctgttcttt
     8041 ttggaaagaa gcaaattgct cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac
     8101 aatatccgtg ttacttgcta atagatctag agcgcctgca agaggatgga tccctcgatg
     8161 ttcttccaaa gttggcaatc cttgatatag tgggcctctc aactctgtat ctcctggtac
     8221 aaaaccttgg acaaggagac cttgaatttt gagccattga ttttttttct gataccattc
     8281 tttgtccagc cctgtttcgg gacgtggata ataattatgc caagcttcaa tctgtgagaa
     8341 atcagcttct gcttctttca attcatcgat atctttaggc gtgatcgtgc tagcattcaa
     8401 actgattttc aattgatgtg accagtcggc aatctgctga ttggaaatat gataatccat
     8461 tcttaaacct gttacaccaa ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc
     8521 tcgttccaat gcctctcctg aaatatcgac catcaattct agtttattct ttttcgcttc
     8581 gcttcctagt tcgatcaatc gcttttgata tgccgaaaca tcatcttctg gtatatgcat
     8641 ggaagtgaaa ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc
     8701 ttctgacaaa gaatcgttca taaatattga aaagccaaac atattatccc tctttttctg
     8761 tatccgtctt catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt
     8821 tattaaaata attaaatttc atttctaaag gttgagttta tattatcaaa agaattgtca
     8881 taacctaaaa aaggcactgc taagcaaaag cagtgccttg aaatatttta tttaaataac
     8941 acaaaaacat aattactcat aagaaaccaa ttctgtatta tctaccatga atgtaaagaa
     9001 cccctcatta ggcatcggat gaaaataagc attgatcact cttatcaagg cattatggca
     9061 aactaacaga tagacatttt cttcgtcttc gatgcattct ttcaataaag gaacgatccg
     9121 tgcatacaca tctaagactg actccccatt tgggaatcgt acagcgaacg ctaaacgtgc
     9181 tttttgaaag ttctcatctt tgcttggcag tccatcataa tccccaaaat ccatttctac
     9241 caatcgttca tcaacggtca gcggcaagct gagacgatca gcaactgctt gtgctgtatc
     9301 tcttgctcgt tgaagagggg aatggatgat tttcgtgatt ggaacaggca aatctactac
     9361 cttttctgct agtttctgtg cctgcatctg tcctttttca gtcagcggca catctgcccg
     9421 tccgcaaact aatccttgtg cgttccaagt tgtttctccg tgtctcgtta cataaagcat
     9481 gtgattctct ccttcgttgg caaagttccc cttaaccata ccttgttgtt cttgagatta
     9541 caagggttag attctctcaa gagaaataat ctcagtagat actttatcca ggaaggtttg
     9601 atcatgttcc actagtaaca tggcaggttt cacgttcaag atcagttgtt ctaattgttc
     9661 ttgattgaag acatccaaat aattcaatgg ttcatcccat gtatatagtt cagctggctg
     9721 tgataaagat ttagccaatt ccactttttt ccgttggccc atactcatct gctcgatctt
     9781 gttatgaaaa acatctcttt ccatcccaag ctttcggagg ttgttcaaaa atgcttggta
     9841 gtctacttgg tttttctctg caaattccgc caacgttcct cgattgtctt cataattttg
     9901 gcttgcataa ctaatgctca gatgttttgg ctggtatttt tctcctataa ccttgccgtt
     9961 gaatgccccc agaagataat ggataatgga tgactttcct gcaccgttag gacctgaaat
    10021 cgccacccgc tgatgaggct cgattgtaaa atgaattggc tcgaataaca gattttcata
    10081 ccctaattga agatcttcta cgcttaaaag tcgcttatgg tgagacgctt ggctattcat
    10141 cgtcaaagaa tcgatatatt cgatatcttt taatagtttt tctttctcgc tgatctgggt
    10201 ctccatccga ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat cagcacccac
    10261 tgctccttta ttcactcgtc tagattcagt atcgatgaat ccgacttgtt tctttgtttt
    10321 atctccttct cgggaacgag accattcggc tttttcagct gctgttttct ttagcctact
    10381 gacttctttt ttcaattttt cattttgagc catttcaaat tcatcacgaa gttttttctg
    10441 ttcttcatag atagagaaat tcccttgata aagttccagt tgactttttt cgattgctaa
    10501 aacatggtcc actacttcat cgataaatcc ccggtcatgg ctgaccacga tgaagccttg
    10561 ttttttcttt ttcaaataag ccgctacttg ttttctacca gagatatcca aatgattcgt
    10621 tggttcatcg attaacggga aatgagtgtc atccacaaat aaaagtgcca gtaggacttt
    10681 tgtcttctct ccccccgata gtgtgctgaa ttctctccat aagatttcag gatctgtttg
    10741 catcaattgg agctctcttt cgatttccca tatctcaaaa tccgtaatat catttaacac
    10801 gtaataggtt aaacgttctt tatcttttgt ctgttgcggg aaataggcaa attcttgctg
    10861 atggattact tgcccttgat aaggtagttt gttttgtaga atattcagta aagttgtctt
    10921 tcctcgaccg tttcgtccga taagtcctag tttccattgt gtgtcaaaat ttagatttgc
    10981 ttgttcaaat aataatgtgc cttggctgtc gtagccgaat gtcagatttt ttatttcgat
    11041 tttagacatt ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga actggctaaa
    11101 gaggatgcaa gggttggata cacacaaaaa gacccataca tcgaaaaaac caagccaatc
    11161 ccgaaaatct gcacactgct tccttgtctc cacaaggtac agtctgaatg cagctttcat
    11221 caatcgactt aatttttcaa tgcacggatc cctcactttg tcctatattt gaaattacga
    11281 tagcataaag aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt
    11341 gtctgtccta agatttttgt tttttctaat gtttgcctgt gttccattgc ttcttctaaa
    11401 cctattggac ccaaatgaat acttaagaca attgggagaa aatcagaaag tccttctagc
    11461 tcttggtctc tacttccgca aatagcgatt attggttttc gatatttttt tgcaagcact
    11521 gctacaccgt atggaacttt tccgtggatc gtttgctgat cgatccgccc ttctcctgta
    11581 atgatgacat ctgcttgttc gatctttttt tccaatccag tgatttggct gactaaatcg
    11641 aaaccagaaa ccatcttcgc tcccaacaaa aaaagggctc caccaatacc gcctgctgct
    11701 ccacttcctg gaatatcatc taaatccacc tcccagaaat cttttatttg ctgagcaatc
    11761 tttcctgctt gtttatctaa gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca
    11821 aattgccaag cagctccttg ttgacctgta tatggattgg taacatctgc agcgataatc
    11881 aattttactt tagaaacaag aacgtttctt cgatcaaaat gaagtttttt agtagataat
    11941 aaaggatttc cttctgtgta cccttctaaa gaagcaccca aggattggag taatcctaga
    12001 ccaccatcgc tacacccgct tcctcctaaa gtgacaataa tttgttctac ttcttgaagc
    12061 agtgcatctt taataagttc tcctaaggca taactggaag caagtcggat agtttgatcc
    12121 gttggtttat caagtagatc taaccccaat gcttgtgcag attcgatgac tgcggtgttt
    12181 tttccttgcc aagttgtcaa taaatagctg atttttcgct gacggaataa gagatccatc
    12241 ccatcaatgg tacgccatgt tcccctgagc gcgtaataga ttgcttctgc agtattttct
    12301 ccgccatctg caatcggcac attgattttt cttcctttac gatcttccca gccatttaga
    12361 actgcctgat ttagttcaaa tgaagaagca cttcctttaa atgaatcaat tgccgccaaa
    12421 atatccatct gtatccctca ctttcttctt aagtaagaca ctttttttca tttttttcaa
    12481 acaaaacaat gctattttct tccatttttc tatatactaa aagggaactc attgaataaa
    12541 ggagagaaaa aacatgaaaa tcgcaatagc tggggccgga gcaatgggca gccgaatcgg
    12601 attgatgcta catcaaagcg gaaatgaggt cctcttgatc gatcgttggc ctgctcacat
    12661 tgaagccatt cgtacaaatg ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa
    12721 actgccaatc tattcaccag aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt
    12781 agcattaaca aaagccaatc aattagatga tatgttttgt tcgatccaat ccattattac
    12841 tgacaatact tatgttttgt gtttactaaa tggattaggt catgaagatg tactggaaaa
    12901 atatgtgcca aagaaaaata tcttatttgg gatcacgatg tggacagctg gtctagctgg
    12961 tcccggaaaa gtcacattgt taggtgatgg agaaatcgaa ctagagaact tggaaccaga
    13021 aggcgaagca tttacaaaaa aagttgtaga agtctttcaa gaagcaaatt taaatccgat
    13081 ctatagccat aacgttcgtt attccatttg gcgaaaagct tgtgtaaatg gaacattgaa
    13141 tggtttatgc acgattttgg attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga
    13201 gagcatggtt cgaacaatcg tatctgaatt tgcttctatc gcagcaaaag aaggaatcat
    13261 actagatcaa gaagaagtgt accaacatat tgcttctact tatgatcctg acaacatcgg
    13321 gttgcattac ccatctatgt accaagacct gatcaaaaac catcgtttga ctgaaatcga
    13381 ctacatcaat ggtgccattt ggcgaaaagg acaaaaatat gatattgcta caccatattg
    13441 tgcctttttg acccaattag tccatgcaaa agaaggaatc cttggcgcag aataaaaggc
    13501 tctctgatga gaatcagctg aatcagcagc tggttctctt tttttaagcc caataaaaga
    13561 tcaaaaagaa aacacaactc tcatgatgcc ataagaattg tgcttgtttg tttcagtaca
    13621 taatctcttc aaaatgtagg ttttttattt gttttcaaca gaggagaggt catattcgta
    13681 ttgctcataa atagtggaat cgttagtgat aaatcgtttt gcccaatttt ctgcatcata
    13741 aaattccaat agctctgata ggattggctt aaatggtgta tgcagaaatt ccggtaattc
    13801 atctaaatca cgttctcgaa gcaaaaaaat attttcagga cgataaaaat gataatgagc
    13861 aatcgcttga ttatccgtca ctagtttctt ttgaaaatac atggattcaa atgtccgcaa
    13921 agtcactcct tgctgaccct cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt
    13981 tttcaggtac tcttcataag taagatatcc aggttgttca ttccctcgat gtggaagaat
    14041 gaaatagttg gtcgtaacat ctctttttat aaattcctgc ttatagcttt ctgctcgttc
    14101 ttttctccca ttattcgttg caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa
    14161 attcatatgt ttcgaataaa atgaactatt atgtttcagc ccaagttctt ttgcttccaa
    14221 aaaatcaaaa tgataaatct catcgattgc cggttcatct tgtgcctgct tcagcatcgc
    14281 ctcgtgttct gctttgaagt gattccaaaa aaataacaca atttttgcat tggtaaatgg
    14341 gcgcacgcgt cgagccatct ccatatcaaa tccataatct aaaaaaacga tcagctgata
    14401 atttgccaac tgttgtctag ctgatttaat gaattccgac tgtagtaaat cttttccgga
    14461 ccaaacatct aaagagcgga tggaaggcaa aaaataaagt gatttatttt ttgcaactag
    14521 cagtacgtca tttttgttca tcttttccgc ctcactttct ctgtcacttt ttcctatgat
    14581 agcctgttta aatagtaaaa agccagtaaa acgtttccta tatttgtatg aaaatttaac
    14641 atttgtatga aaatatgatg aaataaatca ttcttctatt aaaattcgcc taaaagtttt
    14701 ctgaatagtt gtaattgtct gaaaatttgt tatcattagt cattgttaaa tattgctatt
    14761 tattcttaat ataaattttg attgtgaatt taaaagaaag aggtcattta tggaagataa
    14821 aaaattgaaa aaacaactta gctccagaca tatcaccatg ttagctttag gtggagcaat
    14881 cggtgcaggt ctatttaaag gaagtggcga agcgattggg atcgccggcc cttctgtatt
    14941 gattgctttt ttgattggtg ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat
    15001 cgttttaagc ggtggcgaca cgcatcacgg attatctggt ttgatacgcc cttatctagg
    15061 tgctcattct gctgatttta ctgattgggt ctactggtct atgtggatta tcaatatcat
    15121 cgctgaagct gttgcagctg cttccttttt gcagttgtgg ttcccgcaca ttccagcctg
    15181 ggtatttgtt tttatgttgg ctgttttaac tacgatcatc aatctttatt cggtacggtt
    15241 attcgcagaa acagaatatt ggctggcttt tgctaaaatc agtgtcatta tcttactgat
    15301 catttttgct acttatcttg tcggtcagca aatgttgggc acaggcgttt tccctaccct
    15361 tcagcaattg actgaccatg gtgggtttac tcctcatggt atgaagggaa tcatcagttc
    15421 tttgcttgta gttatctatt cttacggcgg ttctgaactg attgctatca cggtaagcga
    15481 agcagatgat cctaaacgtg cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat
    15541 ttccttttac atcattcctt tgtttttgct tttgattctt tttccttgga atactttagc
    15601 aggcacgaat gtcagtcctt ttgtgatggt atttgaaaaa atgaatatcc catttgcagc
    15661 ggatatcgtc aattttgtga ttgtccttgc tttgttctca tctatcaatt cgggtgtgta
    15721 tgcatcttca agaattttgt tcttccgttt gaaggatcga aaaggttcct ctagaaaact
    15781 agctgtttta aacaagcatc aagtaccgca acgtgctgtt ttattttgta ccagtgttct
    15841 ttatcttggt gtagctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg
    15901 ttccctttcg tatacagtat tattgatctg gatcattatc agtgcggcat cctttgtact
    15961 ggcactaaga aaaggaacat tatgggataa aggaatcagc ttttttgcat tagctgtctt
    16021 aggattgatt ttccttggta tcctgttcac gaactccatt ggggtaactg tattgactgg
    16081 cctgctttac ctgttcattt atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac
    16141 gaatgaatga aatcgataga aaattttatt ttatttatgt ggaatagatg gagctattga
    16201 agaaaggagt tgtgacaaaa gtcttgttgc aactcctttt tccgaataaa cagtctcatg
    16261 acctcttggt aaacggtgtt caaaaaccct ctgtgcaaaa taattgattg ttctccatgc
    16321 tcatgacatg atagaagaaa ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt
    16381 ggaagttttt gttgatgatc ggtgtttcaa cggtagttat gtttggtatg atgtacttga
    16441 atgtctatgc gctcgatcat atcttcttca gtcgtacacg agtattcatg gcgttgatga
    16501 tgggtgcagt catggcagtc atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa
    16561 agataaatgt tatgatctta gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg
    16621 tacgaagcca aactactgtc aacgacacag cttggatgaa ggccatgatt cctcatcatt
    16681 ctatcgctat cctggcaagt aaacgagctg atttaaaaga tcctgaagta aaaaaattag
    16741 cggaagaaat cattcaggca caagaagaag agatcgacaa aatgaaaaaa atgatcaaag
    16801 aaatggaatg accattatcc gtccaaataa aaaaagagga tgttccagaa gttatcagct
    16861 tcaagaaata agaagaagct acttctggaa cgccgtgaat aaggggttgt gaaaaagctg
    16921 ccctgcatca agtaataaga acagccaaac aaaaatagct tctcctattt ttcgtttggt
    16981 taggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt tgtaaaatca
    17041 gcgttctgac ctgagtatta gagagacaaa tggaaaaata gttcctcata ttttgccatt
    17101 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa
    17161 gtcgcttagt ttcgcgtatt aagaaaaatt ttgtgaaatg gctttccaca tttttgtata
    17221 aatttggctt aataccgaga aactggcttt tgaataccgt ttattcgtaa aagggattgt
    17281 gacaaaagtc ttgtcacaat ccctttttat gtttttattg ttctaattgc gctgtcttgc
    17341 ttactgcttc ttgcggtttc acaaattttt ttgagattcc ccaacctgtg aaggcactga
    17401 ccatcgaaaa cacaggagaa agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag
    17461 gaataccaaa tgtacttgct acgaaacttc cggcaattcc ccaaggcact aagtagttga
    17521 ttactgttcc accatcttct agcgttctac ctaatacagt tggatcaaat cctgcttttt
    17581 cataagtttc tttaaatgca ttaccaggca gaataacaga taggaattgt tcaccaataa
    17641 aaatatttac tccgatactt gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca
    17701 tctttcttga tactcgttcc atcacggttg agatcaagcc aaattcgatc aacaatccac
    17761 ctaatgacaa agttagaata atcaatgaca cagtaggcat catactttcg attcctccgc
    17821 ggctaagcag tgaatcgatt tgttggtttc cagtatttga aacaaaccct gattcgatga
    17881 ccccagccaa cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt cctactgcta
    17941 cagcgatatt caacaaaatc gttaaaattg ccggcacctt tttccatgca cacagtaaca
    18001 ttaaaaataa aggaatcagt gaccagattg aaatagtaaa gtttgcttct agaacatcag
    18061 tcacttcacg aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa atcatgaata
    18121 aaaagaaaga aacaacaaat gccgggacag ttgaccacat catatgcttg atgtgtttga
    18181 acaaatcggc atcaacaata gctgctgcta gatttgttga ttcggataat ggagacatct
    18241 tatctccaaa aacggcacct gaaacaatag ctccaacaac cagcgctgga tttaagccta
    18301 gtgttgtccc aataccgaaa aatgcaatcc caatcgttga cataacagta aaggcactcc
    18361 ctacagcgct acctacgatt gcacacacaa caaatacaga agggacaaac cactggacac
    18421 tgatcaattt gaacccaatg accattagag ttggaatgat ccctgcttgt atccatacag
    18481 caatcaatga accgactaaa ataaaaataa agattgggat gattcctggt ttgatgccat
    18541 tgatgattcc ttcgtgcagc caatctgtcg ggtatctttt tatcactgca tacaacatga
    18601 ccattccgat cgctgctaag atcgtgatat taggtgagac ctttaatccg acaacgccaa
    18661 tcgcaatgcc taaaacaata accattaata caagtgttgc ttctaaaaaa cttggtgttc
    18721 ctttttttcc catgataatc tctccttttt tagttgttct tatgttctaa aaaagttaag
    18781 atatcggatt actccgccgc aattcatccg cgattcctcc tttataaaaa taaaaagacc
    18841 tgttttaaac taaggacgaa tgactccgcg gtaccacctt aattaaaaac agatctgttt
    18901 ttcactttca aacttttatt caaatgtatg gactttccat gtaattcgtc aattaggctt
    18961 gatcggttca cagcgaccac cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa
    19021 agttgacttg tacgaagttt accttccata agatcatttg tcaatgaaaa agaaaatatt
    19081 ttttcatttt tctgaaaatt atttttataa ctttcacaca caagtttatt ttttactaga
    19141 aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg atgttcttgc ttgattcttt
    19201 ctaatagctt tggctctttc aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa
    19261 tcgcaataga agcaaggcct ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc
    19321 cggcaatata tgtctcagag attgctaccg taggctggat tgttggtatg acttggctga
    19381 catttcctac atcagaagaa ccgagacttg ttctttgttc cgtttctatt tcttcatctg
    19441 gaacacctgc ttcttttgca tgctggaaga ataagtcatc aaaagaagga gtaacgatga
    19501 catcatcaac tgcattttgg aacaatccaa actcaaaagt cgtgcccgta cttatcgcag
    19561 cacctcgtac gatattttcc actttttcat atactttatc taatgtttgc cgatttcctg
    19621 cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac gttggcagct actcctccgt
    19681 ctgtaatgat cccatggata ttgacatctt ttggcaattg caagcgtaga gcgttgattc
    19741 cattaaacac ttggatcaat gcttccaaag catttactcc tttttcaggt gctgctgctg
    19801 catgggaagc gacaccgtaa aatttgatgt cgactggatc attcgctaaa gattctgtcg
    19861 ttttcccata acgaaaagct ggatggacgc ataacgctac atcgacatcc tcaaaaaaac
    19921 cttctcgaac aaagctgcct ttagctgatc cgttctcgcc cccttcttcc cctggggtgc
    19981 catatacacg gatttctcca ccagtttcat caatgaattg cttcactatg ctggcggcta
    20041 atacggagta agtaccaaac aaattatgcc cgcatgcatg accaatacct ggtaaagcat
    20101 cgaattctgc caaaaaggca attgtgggac cgggtttatt ggaaatataa cgtgcatcaa
    20161 aggctgttcg gtgtccagct acttcttttt ctacccaaaa cctttctttt tctaattgtt
    20221 taatcaacgc tgctgaggaa taaaactcat agttacttac ttcaggatgt tcgtggatat
    20281 ctagagcaag tgcttgatat tctggtaaac gatattggat tagatccttc aattggtttg
    20341 ttgagattgt tgttgtcata aaaaattcct cctatgctag tcatttttct tattttctcc
    20401 cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt tttctcgatt actttgactg
    20461 tgtcttctgt ttgataagcg tctactattt tttgatacgt tttattgttt tcatcttctt
    20521 ttcttgcaac gatgatattc acataaggac gtgcatggtc atccactggt tctaagaaga
    20581 tggcatcaga agcaggatta aatccagcat ctacagctac accgctattg atcactgaaa
    20641 tgtctacatc tgacaaagca cgtgctgttt gggaagcatc taattcagaa atgtctagat
    20701 tttttctatt ctcggtaata tcagaaacag tgggcgcttg tccttttgcc ttatccactt
    20761 tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccatttgta acatcattag
    20821 ggattgctac ttttgctccg tctttgatat cagctacatc cttcaaagtg ttagaataaa
    20881 ttcccagcgg tgcattgaca gtatttccga tcgatactaa atcagttccg tgttctttat
    20941 tgtaattgtc taaaaagaat tgatgttgga aagaattcaa atcaatcgat ccatctgcta
    21001 aagccacatt tggttgattg taatctgtaa atttcacgta ttccaaatta atgttctcct
    21061 ttttcaaacg gtctttcacg ttgtcccata catctgtatc ttctccaata atccctagtt
    21121 ttacgatagt ttcttttttg tcagttgtat ctgcttttcc ttggctacac cctgccaacc
    21181 aaataattgc tacaaatgta atgactacaa aacgaataat ttttttcatt ttttctcctc
    21241 cactttcaat gtgtaatttt tttaatcagt tgattactga taaattgact taaaaaaact
    21301 aagattagaa tcaataatgt agccacaatc gttacatctg tttggaatcg attataccct
    21361 ctcgatatag caagattccc taatccgcct gcacctactg ctccagccat ggtagtcagt
    21421 ccgatcacgt tgatgatagt caacgccgag acccgaatga taccaggcaa accttctaac
    21481 aaatacacac gtaaaatgat accgattgga ctggttccca tcgcttctgc cgcttcgatc
    21541 accccaggat caacttctag cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact
    21601 ccaaaaatca atggcactaa agctgctttc gcaccaatcg tcgttccaac taagaaacga
    21661 gtagtcaatg ctaaaagaga aagcaagata atgaaaggga tggaacgaat gatattgatc
    21721 agcttatcaa gtatttcaaa tagcagacgg ttagctagta atccattagg gcgtgtcact
    21781 actaacgtca cacctaagat gattccaatc atcccgccaa tgaatgctgt ccaaaaagac
    21841 atatatagtg tttgcttcgt cgcttcgaca aattcatccg gaatcaaata aacattgggc
    21901 aaatatgttt taaagaaatc agtcatttta tcttcctcct tcgatcactt tgatacgact
    21961 atctaaatcg tttttttgta aaatatttat ttttacacca gatttgatca aataatccaa
    22021 tgcttttttc cgctgtttgg tttctccagc taaagtaacg atcaaactcc ctaacggcgt
    22081 ttcttgtaat aactctacat tgccatataa aatattcgct gttacttgat atttgctgta
    22141 cagatgagcg attagaggtt cgttcgtctg atttccaata taacttaatt ccaccagcca
    22201 tttattggcg atctgatcgg caaaagcaga atgggcaatg atcgtttcta atgcttggtc
    22261 aagatgagta gctgttcgaa taaaatcttt ggtcaattct tcttcaggat tgctgaagat
    22321 ctggatgctg ctaccttttt caacgattct tccatcttcc ataaccgcta ccttgttgca
    22381 gatttctttg acaacttgca tctcatgagt gatcaggacg attgttaaac ctaactggcg
    22441 attcaatttt tttaacaatg caaggatctg aagggtcgtt ttcggatcaa gtgcactggt
    22501 tgcttcatca cataataata ccttaggttc attcgccaaa gctcgtgcga ttgctactcg
    22561 ttgtttttgt ccccccgaca gttgtttagg ataagcagat gctttttctt ccaaaccgac
    22621 aagttccagt agttcattca ctttttgacg acgttcctgt ttcgattttc cagaatactt
    22681 taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc attagattga aatgctgaaa
    22741 gatcataccg atggcctttc tttcttttcg caattctttg ggagagagct gactcagatt
    22801 cgtctgattg acaatgactt ctcctgctgt tggtttctgc agcaaattca tcacacgaac
    22861 taacgtactt ttccctgcac ctgagtaacc gacgattcca taaacatcgc ctttttcaat
    22921 cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga aacatacaga
    22981 aacatctcgc aattcaatca ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa
    23041 atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt accacctcat gttcaagctg
    23101 tgttcacaca caactcctct tcaagtacga tcatactcta gcaagatatc ggtcgcagcc
    23161 gtcatcgcct tacatctatg ctcgggatga attgctcgat ggccattttc caatagtcgt
    23221 tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc gtacttacca
    23281 tttcaatgca aataatattc aaaaaaaaag tccttttact aacataaact atgtcagtaa
    23341 aaggacgacg tatcgcggta ccaccttttt tcggaagttt ccttccctca agcccaacat
    23401 ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca gttccgaatt tgcctactta
    23461 tcgacaaatt attctccaag gccattttca gcttttctta cttgactctt ctcaccagcc
    23521 agagcctctc tgcaaagttt ccaaacttac tttccttttc aacgaaatcg atattttgtt
    23581 gttgggatta atctacatga ggaaaactga aaagtcaagt acgaaattca aattattttt
    23641 tctatattta tttttctgtt tttttattta acatttcaat aacagttcga agatcttttc
    23701 cttccgcaag aagtttcaat ttttcctttt cttcgacggt ataatttcgg acaaaactgg
    23761 aatcaggtat caaaacaacg cctaatcctg ctcgtgaagc agctgttgct cctactaaag
    23821 aatcttccac tgcaaaggct gaactagctt ctaatcctgt tcgttccaaa gctgtcagat
    23881 aaggaatcgg tgaaggcttg ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt
    23941 aatcagctaa tttaaagtga tccaagatat ctgttgctct tttcgtaaca gtggtcgtag
    24001 caatgcctag tttcatatga cgttctttta agtctgccaa tatttctttt gcatatggtt
    24061 ttaagtggat ggctccgctt gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga
    24121 cttcctgtac tttttcaggg ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc
    24181 agttttttcc gccccaagcg tcaacgattt cttttgggat atggatatca aattgctgaa
    24241 atccccaaag ccagccatca cgatacgttt tttctgtgtc aactaacaaa ccatctaaat
    24301 caaatattgc acctttcatc tatacattaa tcctttccaa aacaatacac atagtttagc
    24361 acgactggtc caaaaagaac agaagcaaag aaccagcctt ctcgtttata agagttcttg
    24421 gaatcgacgg atatagattt tcttcacagc tgtcacaagc gccagataag cgatgactgt
    24481 agcaaataac cataaccagt aattgccagg aagtggcatc aagcctaatt cagcaccaaa
    24541 ccttgtaaaa ggaaggacag aaccgacaat aattcctaat gtagtgataa caaacatggc
    24601 aaatgttgcg ttgctttgga taaatggtac tttcggtgta cgcagtgcat ggagcactaa
    24661 ggtttgtgtc catagtgatt cgacaaacca tcctgcatgg aataatgcga tgaaaatcac
    24721 tcgctgtgaa ctatccaatg tatggaaact tccgcccact acagcaggac aaataacaaa
    24781 atacatcaac aagtaagtag taatatcaaa tatcgaactt gttggaccaa agtaaaccat
    24841 gaattcccca atactagatg cattccattt ttttggctcg tccaaatatt ctttatccat
    24901 ccgatcccat ggcaaggaaa tgcaggaaac atcatagatc agattcaaaa acaaaagttg
    24961 taaaggcagc attggtaaaa atggcaggaa cgtacttgca atcagcacgg aaaacatatt
    25021 gccaaaatta gaacttgctg tagccttgat atatttcatg atattcccaa aagtttctcg
    25081 tccagaaaga aggcctcttt ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc
    25141 agattctttc gcaatatcca cagctgtatc tacagaaata ccgatatcag ccactttcat
    25201 tgcaggagcg tcattgatac catctcctaa aaatccgaca gtatgaccat ttttacgtaa
    25261 agctgtcgtc aaacgtgctt tttgctgagg attcaatttt acgaatactt catggtcttc
    25321 agctacttgt gctaattcat gatcactcat ttccgagatt ttctcaccgg taaccaattc
    25381 attgatctct agacctactt gacggcacac tgaacgtgta actaattcgt tgtccccagt
    25441 cagaactttt acagctacac catgttcttt cagagctttc aacgcttgtt tcgttgtttc
    25501 ttttggtgga tcaaggaaag caagataacc aatcaaaacc atgtctgact catctttgac
    25561 tgaaaattct ccaacgacgc ttggattggt cttttgtgcg acaccaatca ctcgtaagcc
    25621 atcttcatta agtgactgta cttttgcaag tacggcttct tttcgttctt ttgttaatgg
    25681 tgtaacagta ccgccaagat caataaattt agaaatgttc agcatttctt caatcgcgcc
    25741 ttttgtgatc atttgtgttt taccagctgg atcagcaacc acaacactca tacgacgacg
    25801 ttcaaaatca aaaggaattt catccacttt ttgataattg atcttatctg tatctaaagt
    25861 ttctgttgct tcatcaatga tagccacatc taacaaattt ttcagccctg tttgatagta
    25921 gctatttaga tacgcatggc gcaaaacacg atcatcttca tttccttcac aatccatatg
    25981 atattcaaga atgattttat cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc
    26041 gattgcacca aagttctgga tggcattcaa atttttgatc actgttcctt ttttggccat
    26101 cacacttgct cctttgacca aattcgtcgt aacgatcatt ggaagcattt caggtgttag
    26161 accaactgct acagatagtc caaaaagaaa agcttctagc caatcccctt ttgtaagccc
    26221 attgatgacg ataactgctg gagccatcaa tgacataaat ttgatcagta gcatggatgt
    26281 tttatggatg cctaattcaa agcttgattg aattggtttt tcagatagcg tttctgctac
    26341 atgaccaaat agtgtttcat ttccaacaga aacaatgatt ccttccgcac ttccactgat
    26401 gacattactt cccataaaga caagattttc gtaacttgtt tcgctttgct gatcgtcttc
    26461 acatactttc gcatgctttt ctactggata gctttcccct gtcaatgctg cttgggaaat
    26521 gaataaatcc tttgcggaca ataaacgcat atccgctggt atcatatccc ctgctgaaag
    26581 ccgaacaaga tctccacaga cgatttcttc cataggaatc tctgtgtact ttccattacg
    26641 ttttacagct gccgttactt tcacaagtga tttcagtttt tcagccgctt gattggaacg
    26701 tacggactga atcaacgtca ttgtcccgct gacgataacc atagtgaaga taatggctac
    26761 accgaaaaga tctttatctc caggagctgc taatacatat tccgtgatga atgaaataat
    26821 tcctaatgcg atcaacacta atgtaaatgg cgtgatgtat gctttcaata tttctacaat
    26881 gaatggtgtt tttttgccat atgaaatcgc attttcaccg taattttctc ttgaacgttc
    26941 gatttgttct tctgtcaatc catttgctga tgcatggaat agttcaaata attcttgttt
    27001 cgtttttcca gcaaaagctg catagatttt ttgtgtatct ttcttttttt ccatctcaat
    27061 ctctcctttg tgtccaaaag acagctgttc tcgctgcttt tggtctttag ctattcatcg
    27121 gatcgataga gaaaaaactg aaaggggctg agaacagata gataatagcc ctggcctttc
    27181 ctctagatcc ttgtttttcg aatgactgtc atccatttgt tctcatctcc tttactaagt
    27241 aattttaatt catgacttcc aatcattttt cgaatagtta tttttccaat tagaccacct
    27301 cctttttatt tttcaccgat ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc
    27361 agcgaaaggt ttgaaaaagc atattaaaaa aagcttctca gatctcatcg ttgagttttg
    27421 acactgtaca acgtaaaaag cagatacctg cttttagcta ccttatgaaa agccttatct
    27481 cgacaattcc tgttctaccc attggcgtct ctcgacgttt ttgggcagta gcctatgttt
    27541 gtataggagc ctcacctaac aaatcttatt cggttttcct tcgttaatat aacaactaaa
    27601 aaagaggatg tcaaatcaat tttccatgtt tttcatactt tttttaaaat acacacgtta
    27661 tattcctgtc tcacgcggtt ttatttgtct cattttccac ctcccgctat aatagaaata
    27721 tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt attatcaagc cagacggcgt
    27781 gagacgccat ttagtcggaa gcatcatcca acgttttgaa gcaaagggac tggccattgc
    27841 tgaaatgaag tttgaaacta tgactcctga attagcaaaa gaacattatc agcacttgac
    27901 tgagcgcagt ttttttgatg aactgatcga ttacatgact tccggtcctg tcgtttatct
    27961 tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag atggttggag caacaaaagc
    28021 tgccgatgct gttccaggta cgatccgagg agactacgcg ctgccgggga ctgaaaacat
    28081 cattcacgct tctgattccc gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc
    28141 attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg
    28201 tctagttgct gggttcagca atggccagca gttttttctc taaggacctc cgttgattct
    28261 ttcctaacat atattcgagc actgtgaaga attcttggta gtctttcgtc ttttcttccc
    28321 ctaaagcagc agaaatcaat ctttgataat aatctgccat caatgtttca tagatcactg
    28381 tacttctttc tttatgtgct aagctccgtt tcatatcttc attcacacta cgtgcctttt
    28441 tcaaccgacc attttctata taaaacgaag tcaggttcat taaaaaagta aagagatact
    28501 gctcatgatt gttcaactgt gctaatcgtc gcattctttt tttgattttc aaataattgt
    28561 tctcaatgaa ccgatcactg aatatccaag gaaaatcagc tagaatagat agttcaaatt
    28621 gaccatagtc tggtatctgc atcaagtatt cttcaatgat tcggcattca taacgtaaaa
    28681 attcaaattc tgtctcagat acttctgttt tttcgaccat gtgaagattc aaaaccaaat
    28741 aatacagatc ttctgttttc ttaaaatgcc tacggttatt gctgatctct ttataaggat
    28801 caaagctgtc ttcttgtacc cgatctagca ttcgtctttt tttctttaac tttattggtt
    28861 catccagctg gtttcgcatc cattcatatt ctgcaagtgt catgttcatt cgctctaaaa
    28921 atgatctcaa tgttacaaat gacatttttt tcactgaatt tgtctcaatc ccgactagag
    28981 aagtcttaaa acagattcct tccgctaatt gggactgtgt caacttcttg ttgactcgaa
    29041 gtttcttgat caattcacca tcatacataa tagttctcct ttttaattta aatttttcca
    29101 cattcaataa atagatacgt taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt
    29161 tttcataaaa aataccaacc ttcaagcatt agaatgttgt ctaactactg aaggttggtt
    29221 tgattgttta ttgattattt ttttgctgtt caacgtctgt tcttgttact ggatataagt
    29281 tggttgttgt tttattacca ttttttgaaa agacacttgt cgaatgttcg cctttttgct
    29341 tttcgattgc ttcattacgt tccgcttcat ttttatagtc atattttttc ggatcaatcg
    29401 tctcaaatcc gtttggcaca taaaaccgca agagattttc ctgattcagc ttatctgaaa
    29461 gacttaacgc agtcttgact ttttcctgat ctttggagat ttcctcatcg attcctgctg
    29521 ctttcgtatc gatgatttct cctgtctgat tattgtaagc ttttcctcct aggacggtgt
    29581 atttgggtgt cacaaagttc ccatttcgaa aagcaactac ttgatcatgc tgagaagata
    29641 ataagtctgt accaaaatga agataatctt tatcatcgat tcccaataga tgtagtaatg
    29701 tcggcaaaac atcgatttct ccaccgtatt catgattgac tgtcccttta gtataaccag
    29761 gcatatggat catcaatggc actcgttgca tctgtgcgtt gtcaaattca tcccatgtat
    29821 ctggatcttt accaagagcg cttgctaaat ctttattatc tgtattcgag atcccaaaat
    29881 gatccccgta aatcacaaag attgaatttt ggtaaatacc agattttttc aaatacgtaa
    29941 aaaattgttc caatgattga tccagataat gtgccgttcg aacataatta tctactgtgc
    30001 tattgccagt attcaaagat ggaaagtcga aattcttgtc atccgtataa taaggcgtgt
    30061 ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg tagatgctca agatattggg
    30121 cagattctct gaataaatct ttatctaata tcccataacc taacgtttcg tcggattcat
    30181 caaaataaga tcgatcaaag aaattgtcat aacctaagtt tttatataca tgatctctat
    30241 tccaaaaact agctacattc ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa
    30301 ggatcgctgg tgcggcttga aagacattgt ctgacccaag ttctgtgaat aatgagcctt
    30361 gaggtaaacc aaatgtgcca gtttccaaca tattttcagc atcacttgtc tttccctgtc
    30421 ctacttcatg aaagaaattg tcaaagctga tcgttgcttg attctggaaa atactattta
    30481 aaaacggtgt gacttcttgt ccgtctacct tcatattaat caaaaactgc tggaaacttt
    30541 ctaaatggag aacgatcaca tttttccctt tagcgatccc aaatgtttcg ggatttggct
    30601 ctgcataatg tttctttgta taatctaaaa cttcatcaat cccattactg cttgcatgcg
    30661 cgcgaacact gctagtcatt cctgttttga ttccgtcata aatagtatac gcatcgattc
    30721 ctagatattt gacaatatat gaacggtcaa atgttcgctg cagcaattgt ggacggttgg
    30781 cttcgctcag cgctagattt cctgaaaaaa taagccctga aagacaggta acagcgatag
    30841 ccatcggttt gccgacaggg ttgcttttga taggtatttt cttgaccttc aaccaaacca
    30901 gtagcccaat gaacacagcg atatccagcc agtagatgac atcatggggc ttcatcaaag
    30961 aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa gcctaataca gatttgactg
    31021 taatgaaatc tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa agcgtattta
    31081 aaatatcaat gatcaaaaga atagctaacg ctggctttgg ctttttaata tacaaagaaa
    31141 ggccaaaaaa caataaggta gtcgcaattg ggttgatcca caagataaag tactggatcg
    31201 taccttccac acctaatgaa aaatctgcat actaggcaat gatcgtcttc agccagaaaa
    31261 agaaaacagc tgtcaaaacg attgctaaac gattttggca caatttttta agttgtgtca
    31321 cgtatatcac ttctctctat tcacattttt tcttattttg ctcctgaaaa atgaaaattt
    31381 cctaaaaaag ttgttatctt aagatttttt tattctttct aaagattatt ccgtccactt
    31441 ttcacagcat aaaaccgctg cgatgtaagc gattaaacta atgaagagat aaaagtcttg
    31501 tggtgaccgc ttcactccac ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa
    31561 gaggctgtga caaaattctt gtcgcagctc cttatttctt gatgcaggac agctttttca
    31621 caacttcttc cttaaacggt gttctcctgc caactcctcg gccacaagtc acaatattta
    31681 taaaacatga gaagctgttt tttgaaattg ttccttgtgg ctcagagtta aacggcaggc
    31741 tcaagacctc ttgatacacg gtgttcaatc agctgaccct cggtattaga ttatgtcagg
    31801 acgctaattt tacaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
    31861 cccaaccaaa cgaaaaatat aaggagctat tttttgaaat tgctccttgt ggctcagagt
    31921 taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc ttcggtatta
    31981 gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt
    32041 caaaacgctg atttcacaac ctctgttttt cattgtccat aggattgata ttgtccttgt
    32101 gatgactcat tcgtaataat tgaggcttga ttttcttcac ttcccgttat cgtttgtcca
    32161 ctgccagaat ctgaaggact tgttcctgtc gactcgtttt tttgagaaga gacagttgtt
    32221 ttcttgactt gttcatttgt ctttttccat tccgctaaac gatcggcgac cacttttttg
    32281 tctacttttt cagaataagc ggtgatcact tttccatttt tcaaacgggc tactttgtcg
    32341 ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt taaattgata ttgtctgaga
    32401 gaaatcgcac taagtacgtc atcgatatta ataaattgat tttcttcttt tccttttggc
    32461 aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta ttttatactc cgcccaccaa
    32521 taagttgaga attctttttt gaaattatct actgattctt tgcttgctac gattttttct
    32581 gtttctttct gagcaagatc tgctgtcatt tttcctgaaa atgctgcgga taaaataagc
    32641 agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc tttttttcct catgctttcc
    32701 tccactccta tttaattttg actattctat tatatacaaa aaatgaaata gaaagacaaa
    32761 aaagttgaaa aagatcgtaa ataattgaac ttctttttca caaaatggta agaatggaga
    32821 atttcacgca tttgctctca actattcttt cttaagcctc aaatccgttt ttatctgata
    32881 cgtctttgaa ccaatgaccc gattttttga ttgttctttt tccatcttgt gctaagtcta
    32941 ctgaaataaa gccatatcga ttcttatatg cattggtcca cgaccagtta tccatacatg
    33001 tccacatatg atagcctaaa caattggcac cttcttgaat ggcttgatgg acatatttca
    33061 gatgagattg gacaaattcg atccgataat catcgtggat catgccatct tctttgataa
    33121 atcgtgcttc gttttctaca cccataccgt tttcagaaat aaagcagtcg atatttccat
    33181 aatgatctcg tacattgatc aaaatatcat agattccttg ttcatagatc tcccatccgc
    33241 gataaggatt cattttacga ccaggcattt cataattgtc gaaataatcc tcaggcattg
    33301 gaccgttttt agtatcgatt ggtgtttctt ttgcttttac ccggcgtggc tggtaataat
    33361 tgatacctaa aagatctact gtattttctt tgataaccgt caaatcctct gcatggatca
    33421 caggcatgag atcaagttct tttactatct caatcaattc acttggaaac tcgcctttta
    33481 cagctggatc aaggaatgaa cgattgaaga acgcatctgc tatttgcgct gctttaacat
    33541 cttcagggtt tgattcgtct cttggatagc ttggtgtcag attcaagatg ataccaataa
    33601 caccaccttg ctccatttca tggtaagatt tgatggctaa cgctgaagcc aacgcttcat
    33661 gatagccaac ttggatcgct tttttcatat ctgactcttc tggataatgc catcgatata
    33721 aataacctgc ttcgactgga acgattggtt cattatgagt aaaccatttt tttactttcg
    33781 aaccaaacaa ttcaaaacaa cttttggcat atttttcata ggcttcaacc gtttctcggt
    33841 tcaaccatcc gcctttttct tgcagataca tcggcatatc gaaatgatac agattcacaa
    33901 agggttcgat accgttttca atcaactcat caaaataacg atgatagaaa cgaacggctt
    33961 cttcgttgat tgttccagat tcatcaggta tcaaacggct ccattggata gatgttcgaa
    34021 atgaattatg cccagtctgt ttcatcaact gtacatcctc ttgatacttc gtataaactt
    34081 gcgaggtttt ttcaggtcct acttgatcaa agaacttttg cggttctttt ttgtaccaat
    34141 aatcaaaaat gttctctcct tttccatcgc ctgaaatcct tccttctgtt tgtgggccac
    34201 ttgctgcaga accccaccaa aaatttgctg gaaattgata ttttttcatc aaagcacctc
    34261 tttttctttt ttagttcgtt ttagttgatt ccatgtatga tattttccat cacaatcact
    34321 ttttcatttt cttcctcttc ttttttgatt cgttttaaca gctgttctgc tgctcgttcc
    34381 ccgattttat ctgttggtgg cgtgatcgtg attttcggaa ggatactcag atcgttttta
    34441 tctaaataat catagtgact gaacgcaata tccttcccga ttctgacacc ttcttgattg
    34501 aaacattcga tggcaccaat cgacatattg taattgctga caaaaatagc agttgcccct
    34561 ttttctaata atgcttttgc cccgtaatag ccgctttttc tagaatagtc tcctgtaaag
    34621 acctctaatt ggatcgatgg atcattttgg accgcttctt tggctcctgc taaacgttct
    34681 cttgctacgt agtctgtttg agtggcagca ataaatccga tttttttatg tccttgttca
    34741 ccatgatgag cgatcacttt tgctacactt tctcgatcat tcactaatat tgtatcaata
    34801 cctgctggtc cttctggaca attcaatgcg ataactggaa tactcatttt tgtgagcatt
    34861 tcaaatccag accactcttc ttcggataag aagacaatca gcccatcgat cacatgcttc
    34921 atcaggtatt caattttttg ttcaattttt tctggctgat cggcaaaccc gcttaataat
    34981 aagctatacc cattttcttc agctactctt tcgatccctg agacaacttc cgacccaaaa
    35041 cggctggaaa tcccgtttat caaaagtcca atagagaagc ttcgattatt cttcaaccct
    35101 ttagcaatga tgttttcttt atagtccaac gctgaaatcg cttctgctat ttttttcatg
    35161 ttcttttcct tcagttgctg accattcaag taacgggaaa ctgttcccac tgaaacacca
    35221 gccatcttag ccacatcacg gatcgatgcc atacattctc actcctgcca gttacatttg
    35281 atttctcttt cttgttgttc gattatgatg accgcattct ttgcatagtg ttgcttgtca
    35341 tcaatatcca tataactaaa gtcttcagta gagcaacttc ctctcactat taattatact
    35401 taaacaagta tttgaaccgg ttcattttta atatagcatt tttttaaaag attgcaacac
    35461 ttttttaaaa cgctttctga aagtggcgga ttttttgcct ttttaaaatt ttattctaaa
    35521 atttactcta tttgtgggtg ttttgtgcgt ttttctcttc catccgtttg aagttttaca
    35581 ttccaatggt ataatttctt tatcctatca aatgggggaa gtattttgaa gaaattaatc
    35641 gtactattta ttttgtctgc gggacttttg acattgactg gctgtgattt gtttggcggc
    35701 tctgaccaat cctcggacga aacaaccgat tcttctgtct taaaagcgga aaaagcagat
    35761 accaccaaca gtgtatatac gctaaaagta cgtaaagata tggatttatc cgcgggacag
    35821 ttgacattta cagataaaaa aatggaatgg acacgcacgt atcagacaca aggaactgat
    35881 tcttctgaat catctgaaga aaaaacagtg ctgacagata tcaagatcac taccaaagat
    35941 gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa caacagtcat atttacaaaa
    36001 atcggtaatt accgtttgaa ggatgaggat ggcaacatct acagtctata gtgccgtctg
    36061 ttggatcttc ttttgtttct ccccttcttt tctgctatag taaagaagca agatgattgt
    36121 aaaggagacg aaatggatga tcgatagtcc tgattattgt acatgtaata acaacaaaaa
    36181 tatccataca gaatttaatg accaaggaag cttgagcata tgcgacaact gtcacaaact
    36241 agtcaaagat tcatttaaaa catttgaatc ttatcctgtt caaaattatg taaaaagtag
    36301 aagaagataa acaaatccaa gcaaaactca ctatggctga tttttatgaa cttcaaactc
    36361 agcttcttcc tttgatcaag cttcatcagt aaaacaaaca gtggatcatt tcagtttaca
    36421 aatcaaag
//
LOCUS       DAGCXG010000019        34960 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGCXG010000019 DAGCXG010000000
VERSION     DAGCXG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.020"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            781..1113
                     /locus_tag="MB022_000965"
     CDS             781..1113
                     /locus_tag="MB022_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBT4465978.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1704..2033
                     /locus_tag="MB022_000966"
     CDS             1704..2033
                     /locus_tag="MB022_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465979.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            2020..2361
                     /locus_tag="MB022_000967"
     CDS             2020..2361
                     /locus_tag="MB022_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBT4465980.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2475..2714
                     /locus_tag="MB022_000968"
     CDS             2475..2714
                     /locus_tag="MB022_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465981.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2781..3020
                     /locus_tag="MB022_000969"
     CDS             2781..3020
                     /locus_tag="MB022_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465982.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            3147..3836
                     /locus_tag="MB022_000970"
     CDS             3147..3836
                     /locus_tag="MB022_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBT4465983.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            3842..5008
                     /locus_tag="MB022_000971"
     CDS             3842..5008
                     /locus_tag="MB022_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBT4465984.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            5090..5761
                     /locus_tag="MB022_000972"
     CDS             5090..5761
                     /locus_tag="MB022_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBT4465985.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5814..7790
                     /locus_tag="MB022_000973"
     CDS             5814..7790
                     /locus_tag="MB022_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4465986.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            7803..8555
                     /locus_tag="MB022_000974"
     CDS             7803..8555
                     /locus_tag="MB022_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4465987.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            8571..9332
                     /locus_tag="MB022_000975"
     CDS             8571..9332
                     /locus_tag="MB022_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465988.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            9345..9605
                     /locus_tag="MB022_000976"
     CDS             9345..9605
                     /locus_tag="MB022_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465989.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            9602..11692
                     /locus_tag="MB022_000977"
     CDS             9602..11692
                     /locus_tag="MB022_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4465990.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(11728..13785)
                     /locus_tag="MB022_000978"
     CDS             complement(11728..13785)
                     /locus_tag="MB022_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBT4465991.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(13930..14121)
                     /locus_tag="MB022_000979"
     CDS             complement(13930..14121)
                     /locus_tag="MB022_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465992.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            14309..14764
                     /locus_tag="MB022_000980"
     CDS             14309..14764
                     /locus_tag="MB022_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465993.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            14761..15003
                     /locus_tag="MB022_000981"
     CDS             14761..15003
                     /locus_tag="MB022_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBT4465994.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            15021..15323
                     /locus_tag="MB022_000982"
     CDS             15021..15323
                     /locus_tag="MB022_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465995.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            15392..17446
                     /locus_tag="MB022_000983"
     CDS             15392..17446
                     /locus_tag="MB022_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465996.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            17446..20058
                     /locus_tag="MB022_000984"
     CDS             17446..20058
                     /locus_tag="MB022_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBT4465997.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            20055..22433
                     /locus_tag="MB022_000985"
     CDS             20055..22433
                     /locus_tag="MB022_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465998.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            22494..23036
                     /locus_tag="MB022_000986"
     CDS             22494..23036
                     /locus_tag="MB022_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4465999.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            23114..23446
                     /locus_tag="MB022_000987"
     CDS             23114..23446
                     /locus_tag="MB022_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466000.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            23447..24040
                     /locus_tag="MB022_000988"
     CDS             23447..24040
                     /locus_tag="MB022_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466001.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            24062..26086
                     /locus_tag="MB022_000989"
     CDS             24062..26086
                     /locus_tag="MB022_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBT4466002.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            26086..27285
                     /locus_tag="MB022_000990"
     CDS             26086..27285
                     /locus_tag="MB022_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4466003.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            27301..27945
                     /locus_tag="MB022_000991"
     CDS             27301..27945
                     /locus_tag="MB022_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466004.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            27956..28762
                     /locus_tag="MB022_000992"
     CDS             27956..28762
                     /locus_tag="MB022_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466005.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            28785..29014
                     /locus_tag="MB022_000993"
                     /pseudo
     CDS             28785..29014
                     /locus_tag="MB022_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29035..30108
                     /locus_tag="MB022_000994"
     CDS             29035..30108
                     /locus_tag="MB022_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBT4466006.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            30152..30421
                     /locus_tag="MB022_000995"
     CDS             30152..30421
                     /locus_tag="MB022_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466007.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            30698..30976
                     /locus_tag="MB022_000996"
     CDS             30698..30976
                     /locus_tag="MB022_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466008.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            31015..31437
                     /locus_tag="MB022_000997"
     CDS             31015..31437
                     /locus_tag="MB022_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466009.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            31457..33049
                     /locus_tag="MB022_000998"
     CDS             31457..33049
                     /locus_tag="MB022_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466010.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            33079..33414
                     /locus_tag="MB022_000999"
     CDS             33079..33414
                     /locus_tag="MB022_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466011.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            33555..33749
                     /locus_tag="MB022_001000"
     CDS             33555..33749
                     /locus_tag="MB022_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466012.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPFV"
     gene            complement(33885..>34960)
                     /locus_tag="MB022_001001"
     CDS             complement(33885..>34960)
                     /locus_tag="MB022_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4466013.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT        12438 a         5389 c         7113 g        10020 t
ORIGIN      
        1 attttaattt tattacaaat attacaaata aatagttagt attccggagt ttttagatta
       61 aaagtttatg aaaacaatca caatcgtagg ttttaaattc ataatcgtta gatattttag
      121 ttatttttca ctcaaaaaaa tagggaacta gtgtttgcat atgtgctttt ttgagttata
      181 ataattcatg aagagaggta attcacttat ttccccaagc tgaaaagcct gatattactt
      241 cttttcaggc agtgctgcta atattcaatt acactttttt gtatattttt agttgtttta
      301 aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggacgata
      361 aatttggaca attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctggta
      421 accttcgtcg acgtccacag gtggcattaa gtctataccc cttaatgcgt ttacctttca
      481 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
      541 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
      601 atgttataaa aatgtccgtg gggctctttg ccgtatgacg aacgctgaaa ttagagggaa
      661 cgcatgtttg aatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
      721 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
      781 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
      841 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
      901 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
      961 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     1021 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     1081 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     1141 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     1201 aaaaataagg agtagacttg atctatcaac aattcccttc ggggagtgac ggggtaggtt
     1261 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     1321 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     1381 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     1441 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     1501 tcgtgtgctg cagtacatgt agatacttgc gaggacgttg atcattggat caatcgagca
     1561 tttaccgctg tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     1621 tatatttttg agtgaacgaa aaacgataaa gatattcgtt atgaagtaaa actaatttag
     1681 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     1741 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     1801 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     1861 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     1921 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     1981 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     2041 atacgattgg gcacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     2101 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     2161 gatttatacg atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     2221 gatgaaggta atgaatggtt taaaatgacg cttacggatg ataatggaga cgaattatct
     2281 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     2341 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     2401 gagtcacgac gaataataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     2461 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     2521 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagatgta ttcaaatccc
     2581 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     2641 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     2701 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     2761 caagaaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     2821 aagaaatgga agaaaagttg ctaaaaatga tgaaacaaaa acaccttaaa agattatctg
     2881 tcatgcaata tatcaatgat atgaagataa caggaaaaga gaaagcttgt ttgttaggtt
     2941 caatgaaaaa ctttgaacag ttgaggcgaa cttatgtaaa aacaggttcc aattgccaac
     3001 tattattaga agtttcttga cgattatgat atgaaaatga tcaattatga tgtggcattg
     3061 ttattgaata aaaagaatag taaaatttaa catggtttat cttggttaga tgtaggtaaa
     3121 ttaattcata tatagaaggt agatagatga ataaaaacaa taaaacttta tttaatcctt
     3181 acgtgggcgt aatcattgtt tttcttcttt ttagttattt tgcttatcaa ggttatcaaa
     3241 aggacaaaat cgctgaaaaa gtagatggtg taacaaaaac aatccatatt caggaacagg
     3301 ctaaaaccta tcaagataat ttaaccagtt taacggatat ttctaagaga caactgatta
     3361 ctaatcaaaa acaggcatta gaaaaggatc tcaaaggtga aattgctgga aaacttgttt
     3421 tttcttctac aggatttatt ctccctgttt ttcaaggagc gaatagcgac acgcttagtt
     3481 taggagtagc ttctacttat tatctagata ctgaaatggg aaatggaaat tatattcttg
     3541 ctggttataa cgtagataca ccaagcgttt tattatccga tatgactgat ttatctgaag
     3601 gagaagaaat tgatctaatc gatgctcatt acaagtatca gtatgaagtc tccaaaagag
     3661 gaaaaatttc tgattacagc caggtagttc aatctaagga aaacaattcc tttttatcct
     3721 tgccaaaagc aggtaaaaaa ccactactca cactgttagc ctttcaagca aataatatga
     3781 aaaagtgcga tgtgatccag tgtgaattag tcaatgtaga aaaaatcaat aaataaggga
     3841 agtgagtcac attcaagaac ttgtagaaaa attgatcaat aaaatgagtg ccatttttct
     3901 gttactgttg gtatccattc tatatttaat atggacggtg gggttaaatc ctcctacgat
     3961 attatttatt tatgataatg ctgcacagtt agtgacgttt ttcgtaggct gtttgtttct
     4021 tttgaatctg tacaaggtaa agagaaacga tctattcttc attgcagcag gcattctttt
     4081 ttaccttttc ttttcttttt ttagaagcta ccgaggagat actttctatg gtgatctgat
     4141 cgttccgaca attattgcta ttattttgat tgtaaaatgg acgaaattta ctgtaattga
     4201 tcgtagcttg tatcttgtag tattctatgt ctttttactc gtaacggttt atcgagtatt
     4261 tactgagata ccagtaccag aaggacaaag tatttggaat ccatcgaaca aattatcaga
     4321 tatttggatc aatacaaata cgataggtag ttctttaatg acattagccc ttcttatcac
     4381 aggatttgct agttcatttg gaaaatggta catgcgattg ctatcattac cagcgttgat
     4441 tcttgctggg gcaacgattt gggtttgcca atcgaaagca gcattatatg cgcttgttat
     4501 cttcattatc ttagaaattt tacctaagaa ggtttttcga aatacacact tgtccttttg
     4561 gatgtatgca gcagtttcgg ttctagcttt accgatttct tattttgctg caatgtcaga
     4621 caaagttcac ttatttacag gacgagaaga aatttggcgc aaattctatg aaacattagg
     4681 cgaaaaaaca ggacaaatat ttgtagggat gaaacctttc tttttccata gaggaaatca
     4741 gattctaggt aatcataata cgtatcacgc actcgtcaat cactatggat tgattggttt
     4801 tgttctatcg attattcttt tgcttagttt cttatggttg ctcgtaagaa aacgtgagct
     4861 gacaaatgga caactaacat tcttgtgggc ttttttagcg atcatgtgtc aatcgttcat
     4921 ggaagaaaca ttaacgacaa tcagttgggt gccaattgta ttcttaatgt tagcaatggc
     4981 tacacaccaa tataaaacca ccagataaat gaaatagaat agtaataaga gaaaacactt
     5041 tttcgttgtg aaaaagtgtt ttttgttttg tctgaaagga ggcagatgga tgaaaaaatg
     5101 gttatttggt tttttaggag tcgctttaat cgtcgtttgc agtgtgtttg gctatgttag
     5161 ctaccaaaaa catgaaggag aggtgtttaa gcaaaatata gaaaaaaaga tgcccgtaga
     5221 tcaaatcaat gcacacgcaa agagttataa ggaggatgca acgaacgtca ataatgatat
     5281 gtctttgggg caaatgttgt ccatacaaaa agaagcaatc aaaatgggcg tcaataaaca
     5341 agtatttgca caaattcaga ttcctgcact tggtttagca ttgccaattt ttaaaggggc
     5401 aaatcaatac actttgagtc ttggagcggc tacttatttt tatgaagatg ccaaaatggg
     5461 caaaggaaac tatgtcctag caggtcataa catggaaatg ccaggtgttt tattttctga
     5521 tattcaaaag ttgtcattag gggaggtgat ggacttagtt agtaacgatg gcgtctatcg
     5581 ttacaaggta acgcgtaaat ttatcgtacc tgagtacttt aaattaatcg atggcgttcc
     5641 tgaagagaac tctttcttgt ctttaccaaa gaaaggagag aaacccttat taacgttgtt
     5701 tacttgtgtt tatacgtcac aaggaaaaga aagatacgtg gtacagggag agctacaata
     5761 acaagaattt aatcaacgcg agtcgttgaa atatgaaatg aggagagtaa aaaatggcaa
     5821 taaatcggta tgtaaaaaaa gctggaatga ttgttggaat gttaggaatc attgcaggct
     5881 gctttcaggg gtatcgagct tatgcagaag atgtcacgca aaaaacgcca ccagaaaggg
     5941 tcaatatcac ggttcataaa ttaatgtacg atcaaagtac gcaattgaac gttgatattg
     6001 acggaatcaa aaatgatggt tatacgcacg atcaatatcc tacaggtgta acgaaataca
     6061 acaaggcaga ttatggtgat gttgagttta cgttaggaaa tattacggat caagtattac
     6121 cgactgaaga ttctgatcta accaacgcga aagtggacga aatcgttaaa gatgtagaag
     6181 acaagggatc taacagtgag tacgttaaga atgcaaccaa taaaattact tcagctgtag
     6241 atgaaaatgg tgagatcact tttgctgatc aaccagccta tgtcaattcc aaagggaatg
     6301 tatatgtggt ttatgaaagt aaatccgctg ctggattagt cacacaaaaa gcaaaaccaa
     6361 tggttgtgat tgcgccaatg acagacaata caggatcagg atttttaaaa gatattcatt
     6421 tgtatccaaa gaacattgtt tctaagttga gttttgaatt aacgaaattt ggtgacgatg
     6481 gcacagctca aagcaaacaa acacctttaa aaggggcaaa atttgagtta tacaagggcg
     6541 aacctggaaa aggaacaaaa ttaggagagc ttgtatcgga tgatcaagga aagctgactg
     6601 caactgattt gacgttagga aaatactact ttgtggaagt tccttcagaa gttgtagtgg
     6661 gatcagatca agaaccaaca gcagaccaat atttgttagg cgcagacgct cgcaacgatg
     6721 cgcacaataa gttaaccttt gaaattacga atgacggcgt gacaagtgat ttaaaagctt
     6781 cctacgtgaa ctataaagca ccagtgatcg ataaaacagt gacaaatgga acaggagaag
     6841 aacattcttt ccaaattggt gacgcagtga attatcaagg tacaattcat atccctacag
     6901 atatcgcagg tggaacagat gggattacag taaatggtgt aaaaagtgaa acttctcctt
     6961 acagtgtatt taaatggggc gacacagcag gtaaaggact ttcttatgtt gctgaaaaag
     7021 cagatattaa agtaacaaat aatgatggtt cagtcgtatt aaaagaaaat actgactata
     7081 aaatcgaaaa ttctgatagt ggttttgtga tcgattttat cgtaaataat ggacaagtaa
     7141 gtgatacagt tgcgaattta catggtcaag atctaaaaat gacttataac atgtttgtga
     7201 atgatagtgc tgaaatcgca aatccattaa caaatagcgt ggatttcgtt tataacaata
     7261 atccatttaa tcaggaagaa caccacgaaa aaacgaaagc tgacgttgtg acttatggcg
     7321 caaaattctt aaaagtcgat agcggcctat ttggaacagg tatcaaagca acaccgcttg
     7381 aaggcgcaga atttgcagct aagaatgcag aaggtaagta ctacggtgga cttactgata
     7441 cagataaaga tggtgtcaaa gaagcagttt gggtagacga tgtagcaaat gcagcaatcc
     7501 taaaatcaga taaagagggt cattttgaaa tcactggttt aactgaagga gattactcac
     7561 tggaagaaac aaaagctcct gaaaactatc aaaaattaac aaaagagatc tctttcaaag
     7621 tagacaaaga ttcatacaaa gaagaaaacc gaatcacgat caaaaataat caaaaagctt
     7681 ccgtaccaat gactggtagc aacggtttcc aatcttacgt attgatctct tgcttgttgc
     7741 ttggggcagg cgcaatttct gcagttgttt actttaagaa gaaagcctag aaggatcagc
     7801 taatgattat cagacaccct aagaaaaagc gaatcatggg taaatggatc attgctttct
     7861 ggttactttc agctgttggt gtcttacttt tgatgcctgc tgaagcgagc gtggcgaaat
     7921 accaacaaaa tcagcagatt gccgcaatcg atcgaacagg aacggcagca gaaacggatt
     7981 cttctttgga tgttgctaag attgagttag gcgatcctgt tggtattctt acaattccaa
     8041 gtatttcttt aaaactacca atttatgacg gaacttccga caaaatttta gagaatggtg
     8101 tagggattac ggaaggaact ggcgatatta caggaggaaa cggtaaaaat cctttaatcg
     8161 cagggcacag tggactttat aaagataatc ttttcgatga tttgccttca gttaaaaagg
     8221 gagaaaagtt ttatatcaaa gttgatggtg agcagcacgc ttatcaaatc gatcggattg
     8281 aggaagtgca aaaagacgaa ctacaaagaa actttgtcac ttacttagaa ccaaatccca
     8341 atgaagatcg agtaacgcta atgacatgta cgcctaaggg gatcaacaca caccgtttcc
     8401 tagtgtatgg gaagcgagta acctttacaa aatcagaatt aaaagatgaa gaaaataaga
     8461 aacaaaaatt atcttggaaa tggcttttag gaagtactgt ttttctttca gtcatgatca
     8521 ttggttcgct atttgtttat aaaaagaaaa aataaaggag ggtaaccaac atgagaaata
     8581 gaaaatggct atttgtttgt gcagccgctc ttttttcttt cactgcctac acacagttga
     8641 cgtttgcaga cagacaagaa agcacggata gcacagaaca aacaatccaa acgtcagata
     8701 gcagcacgat tcaaagcagc gatgaaaccg tcgattcgtc aacagttgat tcttcagaag
     8761 aacctaaaat tgctgaagac gatcaaatag tcaaacagac cattcacgtt caaaaaatta
     8821 tttcttccga acaattagac aaccaggggc aaaagcttga aaaacaagaa ggagtaaatg
     8881 gcgcgaagtt ttctgtttat gatgtatcgg acattttaca aaagatggat gttaaggatc
     8941 tcactactga ccaaattgaa tcccaattaa aagatcgagt gaaaaaacta tccagtgatc
     9001 aactgaagct aatttctaat ggagaaacaa agacaattga tcaacaaaca ggtgtctttg
     9061 aattctcggt agaagtacag gcaaatcaga aacaagctta ttatattgtc aatgaatcaa
     9121 gtcctgaaaa catttctaat agcgaggata ttttgctatt aacacctgtt tctgataaaa
     9181 atggtgaatt cttaaaggat gtgtggattt atcctaaatc agaagcttcg caacctaaag
     9241 aagaagtaaa aaaaatcgta tctactggcg taaagaaaaa cttctttgag aattgttggg
     9301 attttgttac tcacttgttt tctagagatt aaggaagaaa taaaatgaaa ctgatcaaat
     9361 tattattatt caatttttct tgtttaaccg cagtcttttt gtactgcggt ttattatttt
     9421 ctaaactatc tattttgcca agctaccaac tatacctaat gaggatttta aacataccag
     9481 ctgcttccat gccgctagat attatggaaa agctcattag gaatccatta tttactacga
     9541 tcaatttgat cgttattgtc ggaatttcac tagtcattta taaaaaagag gggatgaagg
     9601 gatgaatttt aaacactcgt ggacaagacg gttaaaatat gtttttccca taatgatgat
     9661 gttaggtaca ttgtttgggt taaaaacagc caatgtcttt gctgaaaaag ttattgtcga
     9721 tccgtcaaat ccaatccaga acgtaaagaa taattttatt attccaaaag atgttcctaa
     9781 tgcaaaaaca aatattactg taaacggagc gtcaaaagtt tacaaatatc atgttgatgc
     9841 caacaaaggt ggttttacag acgactacgt aggattagtg gagggaaaca aaaatatacg
     9901 ctatccttct ttccaaaaag aatacggtga aacaaacctt gttttagaag gcgtaacaac
     9961 caacaccaaa gtcaatattg actatggaaa aatcggtacg tataacggga aaaaagtcaa
    10021 tatcaagcta gttctatcta atattcactt atactcggat acgttgccgt ggaatctact
    10081 agataacaac tatacaaaaa ctcattttcg agacgatggc tataaaaata cgaatggtgc
    10141 aatgagtaaa tctaaaaaaa gaacagtact ttggatctca gacaatcttt tctcaggaat
    10201 tgtttaccat tcaacacaaa tgaatgtgca gctagtagcc acatatgagg acggtagccc
    10261 cgttcaattt tcaggagata catttatcag ttttaactct ttgaaccctg caggaggaaa
    10321 aagcacggat cttaaaggag aatatgccca ctatgacaaa atgaatacga cagattggta
    10381 tgtcagaaaa gatacggttc tttcagaatt taaatctttc tacaacaatc tgaacgtggt
    10441 aggtggccac ccaggaggaa gctctaagct gacacaagcg gataatgatt tcaataattt
    10501 gcatgataaa ttaggagatc cgaagtttgg tcaaggtact gtgtccttta aaatatctga
    10561 agcaaatccg acgtttgtga ttggttcttc taatgttcaa acctggttta ctttatcttc
    10621 tgcgacaatc tttagtgttg ttcctgatca acctgaaaaa acaggggttg ataaaaatgg
    10681 aaataacgtg aacgataaaa tgttgcaagt aggagacacg attcaatatc gaatcaaaca
    10741 gaaggtgaat cgattaggag tggatttact agcggtttac gatcgatttg agttaatcga
    10801 taatttacca aaagaagtaa attacgtcga tgcacacgtt gaaagtggta caaataaaaa
    10861 attcgatgta tctggtgaag tgacctatga caaaacaaaa caccaggtaa aatatgcagc
    10921 gaaagctgat acattaaaaa atcgaatgaa gtataacggc gaaacttacg aattagtgat
    10981 caatgtaaaa gtcaatgaat tggccaatca aaatagtgta gccaaaaatc agggaacgtc
    11041 gatcattaat aaagtagaaa aggatacaaa tatcattacc gtttatttcc ctaaaattcc
    11101 agtgaaagaa gtacaacaaa atggaaaaga tgtaaacgga cgaaatgacg gtaagaaggg
    11161 tacgccgaca gctcctttaa acgcaggatc tgaagtacaa tatctagtca cacaaaaatg
    11221 gcacacaaaa ggcgtagacg ctgcgtcaga tcactacaaa caatttagta ttcaagatcc
    11281 aattgaagct cgtttgacat ataaagaagg ttctgcgcaa gtaatagata agagtaaagg
    11341 aaaagatatt acttctgaag gcacactaac ctatgatagc aattcaagaa cgctgaagtg
    11401 ggaagcttcc gctgacttct taaataacaa tcttttagat ggacgagaaa ttcagctgat
    11461 atttacagct aaaactccat tacaatcaga aaaaaatatt gataaccaag ccgtagtagc
    11521 agtagataat gtttctaata aaacaaatgt tgtgacaatt ggggttgatc ctaacttgcc
    11581 acaagttatt gttcctaaaa caggttctac acatttagta acgattttag cggtcagctt
    11641 actattactt gtgttagcaa cttttggcta cgcagttctg aagttcaatt aaacaaaaaa
    11701 acaaggctga aaatcagcct tgtttttcta tttgaaaatg ggattggcac taataaatac
    11761 gttgtctgaa gaaagtgatc cgtctgcaga aacttcataa gcatgggata aaccaacgat
    11821 actttgattg ttgtaaagca agtttgcgtt atgagcagcg ctatctttcc acataaccat
    11881 agcatttact gctcctgtat ctgaaaggat ctcgtcaacg taaaggtaga aactattgat
    11941 attttctcct ttagcaccgt agcagcctaa gctttctaag agatcttgga aagtaccgcc
    12001 gccaggtcgg taatgactga agcttacttg taattctttt gcacgaatat ctgcagcttg
    12061 ttgaacgata tcattgaatt gcattggggc aacgccattt tgttgacggt agctatttaa
    12121 ccaagcaagt tctttttgac gcacatagtt gtttaaatca cttagtgaga tatttttgta
    12181 tttgaaagtg atcgttcgat tttggtcaac attattgatt acacgttgtc catttttatc
    12241 aacggtaaca gatgaatcgt catttctaca caagaagtag ttttgatcgg tcatattttt
    12301 ccaagaagta gagaaactat ctccctcgta aaccgtttgt gtttcagtag ataaaacagc
    12361 gtttgtgtca acgtttatat ggttcacagt gacgttgtat tgatgagtat aggcaaaatt
    12421 gattccttga ttacttgtaa cgttgtttaa agtcacagtg ttgctagagc tttctctagg
    12481 atcaagaaca aaattagtga acataggtgc ttcaattgtg atgctttgtc cataaacgac
    12541 gggataactc ttactgtaat tatagagttc ttggatattt ggatcagtat ccgagacttt
    12601 atttggacga gctttcactt caactagaaa gacttcatca tagtaaaaag taaatgtggt
    12661 atttgtgtta atcgtttgtg tttgtgtttc attcccattt acttgatact gtggtgcttc
    12721 aaagttagtc aagtcggttc tagcttttgc agtgtaactt gtaccagctt ttactgagac
    12781 atgttcttct tttagcagcg ttccagtttg tcgattcata tgacggacag ttaccgcata
    12841 ttctgaaggt ttaggtttgt gttgtaaccc tttaaatgca gaatcaagac tatttttagc
    12901 attatttact tgggtttgag aagctttaga atcgtctagt acttttttag cattggataa
    12961 agcagtctga aaattattcc aagatccttc tgtaaaatct cctttagcag ttgattttac
    13021 ttggttgtac aaggcttgta attccgtttt gttcacagta ggagctggtt ttttctttaa
    13081 tcccttgtaa gcagaatcta atgtgttttt ggcactatcc acttgttttt gagtcgcttt
    13141 tgagtcattt aacacttttt tagcattgtt taaagcggtt tggaatgcgt tccaggtatt
    13201 gtcggtatag tcaccttttt tagttccctt aacgctcgta taaagagctt ctaaagctga
    13261 tttattcact ttgggtttag aggtgtcagc accataccaa ccaatacctt cgtatttcca
    13321 gccatagtct ttggttaacc attgtacttc tttccaactt gcagtgtagt gatgcgaacc
    13381 agaggtaaca cctggaagga aaaggcggta gatcggttta ttattttttt cggcagaacg
    13441 ccaggcaggc ccttcataag tccaaccgta atttttggtc aaccatttca cttcatccca
    13501 actagctgtg taatggtgat cacgaaggcc tttattgtat aaacgataga caaggtggcc
    13561 accgctagtc ggtgcttccc aagcggggcc ttcataatta ccccacttaa ttttcactag
    13621 gtgatcacgt tcttttgtgc tagaggtgta aaaatgttca tggttatttg gattataaag
    13681 gcgatacatg acgttagttg aagcagcgtc agcagatcca gcaagagcaa ctgtggcagc
    13741 aaatgttcca atagctataa cagtttttgg gtttaataat ttcatgtttt caactcccta
    13801 ttaaatgatt aaatattttg tttacattca taatatacca cggtataccg aggtagtcaa
    13861 cggtataccg tggaaatttt gaaataaaaa agccgcccta tgaagagtgt aaggatggct
    13921 gtttatatgc tatgaagaaa tggattcggc gtatttggtt agtttggcag cattatcaaa
    13981 tcgcattttt tcaatcgcac tttttttctt tattaaatcc gcaattgttg attctgcgac
    14041 tgaagaatgt ttgctaattt catatgctgt cttgccagtt tcaaatagta accattcgat
    14101 tttctcttta tcagcgtaca ttgcaatgct ccctcttgat tttttcttta ttatagctct
    14161 tttttgtaat aaatcacaat atcaagagga aaagtttcta tagaaaggtg ggaaattatg
    14221 agtatccgtt ccccgtgtat caaaatgagg tttgttgcta ctttcgttat ttaatatact
    14281 aaaataaaac aacgaaagaa ggaatagaat gaaaaagtta ataatggtat ttacttcgtt
    14341 gattgccatg attgttattt taggtggatg tggtagtgaa aaaaataagc aagatagtca
    14401 agataatggc gtgttaaaac caggaacagt gtggaaagaa gaagtaggcg gacttgttca
    14461 taatttaaaa attatagatg atacaacatg ggaatattcc gaaagtgttt ggcatcctga
    14521 acaagtacaa ataacagttg agaagctaaa agactataaa ggattagaaa gatataaggt
    14581 aatcgattca ggtggagtag atttattcat aggtaaaaca gccttaatag ttccctataa
    14641 caaagacggt ttagaaacgt ttaaatttat tccaacaaac gaaaaagaac atcgagataa
    14701 aagtgaaata ttaaaagaat acagtactaa atctggatac aagttacaaa agactagcga
    14761 gtgattgcta gtctttttat ttatcctgaa ggaggaatcg cagtggcaaa agctaaaacg
    14821 ccatttgaaa aagccgcgtc taaaatctta gaacgtcgcg gtgtatctga aacacagtgg
    14881 aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga caaggactta
    14941 gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat tattaaacac
    15001 taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta acacaaccga
    15061 aagtagtagc tgcggttgat atttttagag cattacaagg tgcagcaact agtttaaaaa
    15121 acaatctgat gatttttgcg gctgtagctg ccgttgcctc cattgttctt ggggggattt
    15181 tcttcttctt tggccgaaga ggagctgaaa acgggaagat gcacgtaggt tatgtattga
    15241 tgggggtagg agttatatta gctgccgcct ctattgtggt ttcgttattt ggaatgtttg
    15301 gacaaacccc acagggacta taaaaaataa atagaaggag taaaaagtac gtggctactt
    15361 tttactcctt ttttatgaaa ggagaatact catgtacttt acgatttcag gcaataccaa
    15421 aaaagataaa gagttaataa aaaaagcaac gaagaaagtg ggaaagcgta ctgccttttt
    15481 accgaaaaca attaaatatt ctgtcattga aacggaagaa cagctacatg tgttaggaca
    15541 agtgttagaa caagagaatc aatcttctgt taaattgact ctcacgttac atgataaaga
    15601 aggaaaaata gaaaaggctg gcacagaata cgtttttggc ccactggatt atcaaatttc
    15661 agaaggtttt tatcctacat tgatggattt gatccgcctt gatgtgttta aggaaacgga
    15721 tgaaaccatg agtgtggaag agtcttatcg aaacgaaaaa ctgctacaag ttttagaaga
    15781 agagttagag agcgaccaaa gaaaagtgag tcaagtgagt gaagtcgaag taaatgatga
    15841 agtaccatgg gaacgaccaa tctttccaaa gaaggagtca aaagaaaaag agacagtcta
    15901 tgttgacgaa caagctgata ttccgactca gaaatcgata tttgaggaag agaacgatgt
    15961 aatgggtcaa ttggaggtag atccctttga agaaacaatg gaggatgaag agacggccga
    16021 agtagaagtt gatctagttg aagctaatga agagccaaca tggactgaag gggattctca
    16081 ggaatatatt gaaccactta tcagtgaaga agtagagaaa aacgaaacag agatacaaaa
    16141 ggcaactgta tttgattata ttttggatca atatccgcca gagcatgaat ggatgaaaga
    16201 aaaaaataaa ttatttttaa atgagcttta ttcgaaatat caattacccc aaacctatca
    16261 agcattttta gagagtaaag aagaattaat ctcacagggt aaaggcacac ttttagaaca
    16321 gttagagtct gttcaatcaa caaattggca tgagcttgca caagaagatt tattagccac
    16381 ttttgaagaa cgtcaacaag aaacagatca agaaataaat gagtatgcga cggaacagtc
    16441 atctaattgg gagagaacaa aagaggaact aaataaagaa gaacaacgag tgattgaaga
    16501 agaagtacaa aaaattaaag agcgatacga aaagaaacgc gacaatgcgt attccttgtg
    16561 tatagagaaa attaataact ttactaaaac taatcaagaa gctttatcga aagaaaaaga
    16621 aaacttgtta aaagaacgta taaaagaacg aaaagaagag ttctaccgag gtttacgtac
    16681 agataagcta aagattagca aagaattaaa tgaacaactg aataaactct atcaaactac
    16741 gactgaagtt cttatggaaa gacacgggca gatccagcaa gaattacaag cacaaattcc
    16801 aaaatggaaa aaagagtatg aaattcaaca acaagaattg aaagaacaag aagaaaaaca
    16861 acgacaagaa gaaaaagagc gcgaacaatt agaactaaaa aaacgtgagc agcatcttgc
    16921 agaacaaaaa gtggcgatag aaaaacaacg gttagagcaa gaaaaggaaa aagaagcagc
    16981 tgctcgtaag gaacgtcaag aacaaatgga tacattgcga caagcacagt tgaattatct
    17041 agcacaacct caaccagtag ctgctgttca aacgccagtg aacaaccaag caaatttaac
    17101 tccttcacca cagcgttcct ttaacggatg gatggtggga tgtattgcaa gtttaagctt
    17161 gcttctaggt ggaggaacag tatttgcttt taatcatatc aatcaatctt cacaggcaga
    17221 ttctgtagcc acaacggcgt caatcaagga agaagcccag gaacaagcaa gaaaagagta
    17281 tgaacaacaa ttggaagaat tattgaatcg taccaatcag tcaacagcag agtcaagtat
    17341 ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa ggatcaagcg attcaactgt
    17401 acactcaaac acttccgaaa gttcagaatc aaatgaggtg aaataatgcg tattagagaa
    17461 acaacgagac gaaaagacaa acgattcaat ttagaaaaga ataggtatct ttctcatttc
    17521 tatcgctata ctccttcagg agaagagcta ccagaaaaac atgacaaacg tatttctcaa
    17581 aagatttttt atagtggctt agcttatctt cctttaaact atgttgttac aagaatacca
    17641 gaagcatttc atcgatttga gattattcca ggaagtttac aaggagtgaa tatttttcag
    17701 ttacaagaaa tgccgcagtt attccatttt gattcaactg gtttttggaa tcctttgacc
    17761 ccaacaacaa gtttaggcac acgtttagtt gtttctacga tttgtgttgt tccagctgta
    17821 cttatgggat tgaaaatgga catgtggttt cgtccgctag cagatgggca aaaaggagat
    17881 aaccgattaa aaacgttaaa ggaaatccaa caaatttatc cgcaaatacc agaatcaaag
    17941 ggaagttttc ctggttatgg cggaatacca attactcatt ataaaaaata ctggtatatt
    18001 cagacggata cagtaaatac ttgtattgtt ggtacttccc gctcagggaa aggccagata
    18061 gaagttttag caaccattga caatttgtcg cgtgcagaaa gacaaagttc aatggttgtt
    18121 aatgatccaa aaacagaatt atatgttgct agtaaagatg aactagaagc tagagggtat
    18181 gatgtgtatg cctttaatat caccgatcca ctgcaatcaa tgagtgataa tccattggca
    18241 ctcattgtga aatactggaa gcgaggagac atagacaccg caacacagtt aaccaatact
    18301 tttacgaata cgatttatta tgatgcaaat gcttcagaca ataaatactt taacgaaaat
    18361 gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg aacaagcgga tcaaacaggg
    18421 gattactcca aattaacacc aaataatctc gttgaactac tttcagaaat tggcggtttt
    18481 aattattatc cagatcctga gaatgaattc aagcagctca atgccttaga tgagttcatg
    18541 aatcagttac cccctggaaa tatagcaaaa aaacaatatg gggcaacaaa aattggatct
    18601 gataaagcga aagggaatat cttgtctacg gcaattacag gattgaatcc atttacccta
    18661 acgaaaattg cgaagatgac ttctcaatcg accattgatt acaagtccat tggttttccg
    18721 aagtatttgg atctcaaatt acatgaaagc ctattaaatc aacgcattgt cgtggaattt
    18781 tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag ttggctatcg tggtttttgt
    18841 gaaatcaact ttgattgtca tttagagcag ggagatcatg tttgcttaaa atacaatcat
    18901 caaggaaaac aatatgttgc ttggtatgag ttttctcaaa aagtattgaa agacgaagag
    18961 ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt tgccagaact agaaaaagaa
    19021 gccgttctaa cattagatga gaagaaaagt aacctattag tagagtctat tcggatgcac
    19081 tataccgata aaccaatggc tatttttatg ttaacgcctg attatgatcc atctaaccat
    19141 atcattgtta gcatctttct tagtcagttg tataaagaat tatctacgca atgtgttaag
    19201 actaaaggag ataaatgtca ccgtaggatt cattttattt tagatgaatt tggaaatatg
    19261 ccaccgatgg acaacatgga aggaatcatg acggttacgg caggtcgtaa tatgttatgg
    19321 gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg
    19381 attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc
    19441 gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat
    19501 tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct
    19561 gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa
    19621 gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt
    19681 ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac
    19741 atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta tgattatttc
    19801 ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt agaagaaaaa
    19861 aacgaagaaa atatgtttta taaaatcgtt caatcttttg agaatgagac ggctaaaaaa
    19921 ttgttaattc aattttacga agaacaagca acggatcgtc ttcaagaata tgttgaaaag
    19981 ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac ggctgttatt ggaagctgaa
    20041 aaggcggtga aagaatgatc attggaggaa ttttctctgg catagccgac tggtggtcag
    20101 gtttattcgg agaaggggat gcagatccca aaaccgtaac gaaagtctta caagattatg
    20161 gtaattatct gcagtatcaa gatgtattca gttggatttg taatactttt ttatggggat
    20221 tgatcaaagg gttatataat ctcaatactt tattagaaaa aacaatttat caaagctttg
    20281 atttaaaaga cttattgaac gccgctggtg ttaatgaact atttcaaagt ttgattagta
    20341 aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga
    20401 gtaaacaccc accgaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga
    20461 tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaaac ttttatggag
    20521 acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa attattcaaa
    20581 ataatgtgat tgatgtcagt acattactag cgactgaccc gaataaagta gaatccttac
    20641 caacagataa aagaaatgct ttaacagcga aaaatttcaa aacagcgaat atcaatggga
    20701 ttattacacc tgatcaagct aagaaaatgt ctgaatcaag tgatattagt acagtacaaa
    20761 aagatcggtt aaaggcatta cagtatcgac tagagttaga cgataatggg aaagaaatcc
    20821 ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta tacgtcaggc tatcgtcgat
    20881 acattagtag tccagggatt attttggctg gggaatcttc tttgtcggta gcctatttat
    20941 ttattttgtt tacaatcgtt acttgcatta ttgagctagt atttaaaaaa ttctatttag
    21001 taattgccgc gtcaacggac tttgagacag gacaacggat gaaaagtgcg atagaagata
    21061 tttctcaatc cttcttgtta ttggcgttta cgattttaga attacgaatc tatacattaa
    21121 tgctttcagg tatcggtgat ttgcatgcgg taggaaagat caatggattc ttgtatgttg
    21181 ttgctttaat tgtgctaact attgcgctat tcaaaggctc tcaatcggtt acgaagattt
    21241 tcggtgttga tacgagtctg aaaaacggca gcaattctct tcttagcatg tttgccttag
    21301 gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct tgcaagtgct ggtaagagtg
    21361 gtttaagcca tttaaatgat attcgtaaga atggatttgg ccatcgaaaa gatattaccc
    21421 atgataacgg ggaagaacaa tcagacgata aagctacaac tgtacctaat agtaagaaga
    21481 cagtttccga aatgttcggg aaagcgaaaa atggtgtttc taaagccgca gaaggtcttg
    21541 gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga tcttacagaa aaagccaaag
    21601 acaccttaga agagaaatta gacggcacga gagctaaaga atttgctgaa gctgtgaaga
    21661 atccatccga agcactagaa aaacaaaaag aaaaagcttc agcagtcaaa gaaggactaa
    21721 aagatgcgta tggtgaagga caagtcaatg cagcagtgaa ggcaaataca acaagtgaaa
    21781 aagaaaatca agaaggtgca gcgattcctt cagccaaaga tcaccaagat gagttacatt
    21841 ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg acaacatggt cagttaccaa
    21901 aaaccgatcc agaacaatat cgttataaat ctaacattaa ttctgaaaac gaaaaacgca
    21961 tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc atcgatctgc
    22021 caaatgataa tggcatcgaa gttggaactg aaaaatcaac gaaaattggt ttgcctaaga
    22081 cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaagagaat gatcaaagct
    22141 ttgttcatca atcacctgca ggtactgttc aaatgaaatc aagtcgtttg cctgaaaatg
    22201 gaaatctgac aactgtttct tacaatttag ctgaaagtgg tgaaacagag aatgcgtccg
    22261 ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat caataaagtt cataatggat
    22321 acagaccagc aaacgtagca tttactgcac cgacaaaaat aaatgtgaca agagaaacta
    22381 aaaacaggaa caatgaattg aaagaacaac tattgaagga tatcaaacca taaaaggcct
    22441 atttaagggc ctttttcttt ataaaacaag tttaaaatgg aaaggagaaa attgtggagg
    22501 atattttaat agctacgttt ataactgcac tgggagcatt tttaacgata tttgtggtga
    22561 ctattaaagg atttaaagat acggatagtt tacaaaaaga tcatcagtct attttagatg
    22621 gtcaaaaaac acataccgaa agcattattg tacacctttc agaaggattc acttatttga
    22681 aagatgctat gcccacattc acagtaaaat taacggaaat atttcattct gttgagcaaa
    22741 tatcgtcaga attgaaagag caaagacaac aaaaagatcg tcaacttggc tcacttaatt
    22801 ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa tcgattaaac gatgaaatgc
    22861 aacgagttca tttggaaaat gaagaattac gcgcacaaaa ttatgattta attcaaaaga
    22921 aaagaaatct ggaacaaaaa attgttgaat taaaagaaga aaatcgtcaa ttgaattatc
    22981 aattacgacg aggaaaaaat cgttctcgtg atttaggaaa tgaattagag ttgtaatatt
    23041 tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct aaaaatacaa aatataaggg
    23101 gtatggttat attatggata aatacattca tatgaaaaat cctccaagat ttagtgggga
    23161 atggaagttt ttcgcctttt tctttttaga tctattaacg cttggtggct gctttttttg
    23221 ggcgaaaata gtcaataatc ttgtccactt accactcttt tttgcactct tgaattatgg
    23281 attatggctt gcgatagcgt tagtgattgt ttggcgaccc tataataacc ctggtcaacg
    23341 ccaaattttt gcgattatcg aatctatttt ctttacggat aagaaccact atcatgcgtt
    23401 agatcctaac cgaaattatc atggtacgta taaggaggat gaataaatgc gagtcaaagg
    23461 acaaaattca gttgataaga aatccatttt gcagtttgga aaaccagaag agcgctctat
    23521 cttggactta tttccatata aatcctgtac agaagaggga gtcttaatta ctagagaaga
    23581 tcattttcag cgttattatc gtgtcgtttc cactgacgtg gagggattaa atgaacaaga
    23641 gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt acgtatgtgc caacaattaa
    23701 gttagttagt ttgaccacag agacggatct aacggaacaa atcgtgcaaa agcggcagtt
    23761 attagataaa aatcgtatgg aacagtcact aggaagaaat ctacagcgat taagaaaata
    23821 tgaaaagaaa ttagtagagg agatccagga attaaaacgg gcagaacaag aacgtcctga
    23881 cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag gatttacaaa ctagaaatcg
    23941 tcagttaatg cgatctagtg gcattttagg gttaaaacca ctctacaaaa aagaattgat
    24001 taagattctt tatcgaatga acaacatgaa tgacgaataa aaaagatgga ggaaatgatt
    24061 catgctagat gtgataaaaa cagctttttc aacgcaaagt aatcaaaagg tgattgatcc
    24121 attaaaacaa cttggtaatc gtgaaataga gattttaaaa tcaaaaggtt atgatttaga
    24181 tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt gatgaagaat atgtgaccta
    24241 tggaaatggt tattcacgat gtgtgacagt ttatcattac acaaaaaatc ctactctttt
    24301 ttggttggcc aatctcatga acaatcctca tacagtggcc acactagatg ttagcacaga
    24361 tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta gatgaattaa atgatcgatc
    24421 agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg gaagaatacc tggaactagc
    24481 ggaatatgcc cgaaaattga cttctaatgg ggaaatttct aaacaagtca aagtgagagt
    24541 acatgtttac ggaaatacga tccaagaagt ggatgaacgg gcgcaggaac tgatcgaaaa
    24601 tctaaaaggg atggattatc gtgcggtgac ttatctgcat aaaaccaaac gagattggca
    24661 atcgatgttt accatgttcg atgaacaagc tgaatggttt ggaaatatca aagggcaatc
    24721 acttccatcg ttaaacattg gcggaggatt cccgttccac caccaaacgc taaaagatcc
    24781 aggaggcaca caatatgggg aaaccatgac aggcggccca tttgtctgga atgccacgta
    24841 taaaaatagt gtacgtttat ctaccaatac cttgcttcta ggaatgatgg gagctggtaa
    24901 atcgaccgtt cttaaaatga tcgcggaagc ccatttagct gcaggagact ttatttgggg
    24961 ctttgaaaag ggaaaagact ttattccttt cttaaaggaa tataacggta ttatggtgcg
    25021 tttagacgga tctgacggga tgatcaatcc tttagagatc tttgccacac ggacgtatga
    25081 tgaagcatcc agtttatatg acgatggttc agtcaaagat ttgaagatca atgaagcagc
    25141 gtcctatcaa acgcacttag acaaagtagt ctatcaggta cagttagtga gtcctcagct
    25201 aaaaggaacg atgaaagcag aattcaagac ctacttgaat cgtttttatg aagagtatgg
    25261 aacagtacca cgcggtttta cttcctcaaa ttcgcgttca aatactgata cgcaagtcac
    25321 agggaaagat ccagaagctt atccgacgtt caaagaattt ttagcctttt taggacaact
    25381 ggaactacca ggggcttccc aagagaaaaa gaatcggaaa gaagaaatgg agtctatcgt
    25441 ggaatcctta tgtgagactt atggcatgat ttttgatggt catagtacga ttcgacacct
    25501 tgaccaacaa cagttggtct tttttgacat tgactctatt acaggcctag atcgaggggt
    25561 acaacaatgt cagatgtaca tggccatgac gttgatttgg aatcaggcat tgattcaagg
    25621 acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa gaacaagact tcaaacgctt
    25681 cttggcgatg attgatgagt gccataatgc gattaatcca gatttcattg aaacggtaaa
    25741 ttatgtcaca aacttccaac gagaaatgcg taaagtagaa gcaatgactg ttttagctac
    25801 acaatcccca caagaaatgg tgccagaaaa tatgtcttct gataacttct cgaaactgaa
    25861 gaaagtcttt gaattttctt ctacgaagat ctttatgcgt atggatgaat ctattttacg
    25921 tcatatagaa ggattagttg gtaaatcaat gacgaactca gaattagaat ccattcctca
    25981 acaaaatcaa ggagacgctg tgatcaattt tggctcaaaa gaaaccattc gtgttcattt
    26041 tagtccgaac aaacgtcaat taaaattgtt tgatggagga aaataatgga aaaccaaaat
    26101 gaaagtttaa tcaaacagta tgttaagcgt cgtgcaaagc ggcgcttatt tttgtgggtg
    26161 tttggtactt ctgcaggttt gattactata ttgatcactg tgtttgtgac cctttttcta
    26221 atacttgcgg caggttctat tgataattct gattcaagta gtggtggcga agcttttaca
    26281 ggagaatatt cggaaggctt acccatttat aaagaaatca aaggaagagg ccctttttct
    26341 gatgagattg cgcaatatgc tgtaggtgca gcagtgaagt ataaattatt accaagtgtg
    26401 atcctttcgc aatacggtta tgaatcagcg tttggtacgt cggcgtccgc aagaaatgat
    26461 ctcaattatt ttgggattac gtggtttgat gggtgtctgt ttcctaaagg aactgcgcgt
    26521 ggaattggtg gcattgaagg tggctggtac atgaagtttc ccaactcaaa agctgccttc
    26581 tcctattacg gctttatggt agccacacaa tcaaatttta atgcttgtgt cggaaataaa
    26641 agccctggcg caagcttatt gatcttagga cgaggagggt atgccgccgc aggaattact
    26701 gaagacagtg cctattataa aaattgtatg tctattatta atacaaataa attgatggag
    26761 tacgatgagt ttgcgattaa acactggaga gaaggcggcg gaactggtgg tacgattaca
    26821 ggaagctggg gcaatccatt cccaggaagt agcttagata aaaattcgtt cagtggagga
    26881 caactttttg ggaaaaatcc aggaggagaa ttccgaccta acgggttcca tgatggacta
    26941 gactttggtt cagtcgatca tccaggaagt gagattcatg cggttcatgg cggaaaagtc
    27001 gtgtacgttg gcaatcctgg tatttcaggt ttgggtgcgt gtgtgatcgt gattaattac
    27061 gatggcttga atatggtgta tcaagaattt gcaaatagta caggaaattc tcgagtgaaa
    27121 gtaggcgatc aagtgaaagt tggtcaagta attgggatac gagatacagc acacttgcat
    27181 ttaggattta ctcgaatgga ttggcgtcaa gcacaaggac atgcattcat tgatgatggt
    27241 acatggatcg atccgttacc atttttgaat agcagcaaaa aatagatcag gagaaaaaat
    27301 atggaaaata aaaccttaaa aatcgtctta acagctgcaa ttagcgttag tttaatcggc
    27361 aatattgcgt tgttttggca gcttaaagaa gaacaagaga caaataccta taaaattgaa
    27421 gaagtggcta aaaacgcgtc tgataagctc aaaaaaaatt cttctgaagc acaggcgaag
    27481 attgacaagt taaatcaaac catcaatgag aaaaaccaag aaatcgatca gctgaagcaa
    27541 caagatggca caaaagcaaa agaggaagta actgaagcac aaagaaagac ggcagaagag
    27601 ttcggtcaat tggccattga taaatcatta agcagaaatg aattaacgga aaaactaaaa
    27661 gatgtcgcaa cccaagaagt gatcgacaaa ttaagtcctg tagacgatga tcaccaacat
    27721 gatgattacg gaagttactc ttttacatta ggtgacgtgc aaacttacgc tcaaacaagc
    27781 agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt atacaatcgg caatcctcaa
    27841 ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa ctgtcactga aaaacaagta
    27901 gatggtcaat ggaaagtcac agactttagt tatttcactc gttaggagga agaaaatgag
    27961 taagaaaaaa ggagcaagac aacgttttaa aaaacgaaaa agttatctta gatggctggg
    28021 gatctttatg attctcggct ttttgattct tgctagtccg atttacacac ataaaaaggt
    28081 agacatacca gatgaagtaa gcacgaacgc atttttaaac acgaaggaac tgacgatggt
    28141 ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcgatcg acgatgaaga
    28201 tcagagctta ttaaaagaac tagctaatct taattatcgt gtagaagtaa agacagctaa
    28261 aggggattat caagcaataa aagccaaagt gatcaaggtg tctgatgatt attttgtagt
    28321 gaagttagca catgtaccag aagaatatat tgctatgcga ttaactatta ttcctgaaaa
    28381 aattgatcca aaggtggata tgcaagaacc gcaagatctg attttttata ttcacgaaga
    28441 taaggtcaaa gatcacgtga aagacgaaga ttatgaacaa catgcgatca attacaaagt
    28501 gaaaggatac aagaaagaac aaaaagcagc acaaaaacaa attgaatcct tccaagcaac
    28561 cattgatttg aatgaagaat tagtgaagaa actaaaagat caattgcctt atcaagtcgc
    28621 agaagatcag gaaaatactg aaaataagat taatagttac cagcaagaaa ttgaaacatc
    28681 gaaaacgcaa atgaaagatc aagaagagag cattcaaaaa cttcaagaaa aaattgatcg
    28741 tatgacaaaa gaaaatattt agaagaatag gtggagtaaa agaaatgaag cgagcgaact
    28801 tggtcggcaa agatactttc gctgagcaga aagtattgat taaagcctat ctcaaagttg
    28861 taaaagctaa agaaatatgt caagaagtga attatctaac acaacaatat ctgagttgaa
    28921 gaatggtaga aagagtttag ataccttaaa attcagatca tttgaagctc tatatatagc
    28981 tgctaaaaaa tattttacag ctgataataa ataaaaaatt aaggtgggtt ctatgtgaaa
    29041 aaaacgtatc aaaagttttt atggattagc tatatcgtcc tatttatcgt tgggtttctc
    29101 gtcagtgtgt ccgtgatcgt cggaacgctt tttcttttgt tctctgacga agatctggta
    29161 cttaaggggt taatgagtac gttatcgtgt gtcatgttac tttatttatg tatacagatc
    29221 aacgaaatta tgccccctca ttggcaagaa aagaagaaag aatttaagga agaaatcgaa
    29281 ataaaatcca atgactggat tgaatacaca tggcttcctc tgaaaagaaa tagcaaacga
    29341 aaggaatttt gggcattgca attatggcca ataggaaaac tttttattta tatggcttca
    29401 cttatggtta ctttaatcgg ttatttggtg gtggtctatg gggtggccta ttacttgtta
    29461 agtgagccaa aacagatcgc ggcgtcaaat attttcacgg cagtaatgac attaatgtgt
    29521 gtcatgtgct tttctatttt tagttactta ctcgtccaat ttatcaggca aaatttaaca
    29581 atgagtaaac ttttcaaaag tttattggac ttacttatct taggcagtat gttaacgact
    29641 ctaacagatc ggttcgaaca atctgaaaaa acggtagagg ggataaagaa cttcttaacg
    29701 acgattgatc aatttataaa tggatatatg tatttgtttg aagtaggctt tattctcact
    29761 ttgtttggtt gtgtatttat aaaactcatt aaaagatcaa tcgaaaaaga aatggttatt
    29821 gatctaattc cgacgctgag cggctatgag tattacgttg attttggaaa gtatgaggag
    29881 ttgatccata cggatatcgt tcacacgagt atccatgaag taaccgttaa gggcatcaga
    29941 tctactttaa aagatggaca ggagacgaaa acaatcgacc gaagaaaaga ggtctacaat
    30001 ttttataaag ttgaagcaag tctacgatat ggcattggga aattgaggtt agatgggagt
    30061 aatattcaac aaaacaaacc atatgtttta ggaaaagtgg tgatttagaa cttcctatga
    30121 aagatgaata aataaggaga atcttttgaa aatgattctt ctttacagaa ttgtgcttat
    30181 agttggcacg gagttaaaaa tcttaaagaa ggagcagtta ttcaaagtga aagaacatca
    30241 taatcagtta ctatgtatta aaacgggtac gatgaacatt caataccgaa cagaatattt
    30301 agaattaacg aatcaaactg attttttgtc gattcgagag caacataagc aagaaaaatt
    30361 aatggctact tatagccaat tgattgatga gtgggaacaa ttcaaggaat ctaaaacata
    30421 aaattccatt taaacacctt attttttaaa gagcataagg aaacgtgtac ccactttcaa
    30481 aataaagtgg gtagataggc tgtgcctatc acgtttctga aaaaaataaa tgaccccaaa
    30541 attttcaatt tatgtgtagg tttatgggta gcaaaaatat gaatgtgggt agcacaaaag
    30601 ttcatttgtg ggtagtaaaa tttaaatatg tgtagcaatg tgtgtagcta cacacattgc
    30661 tacacacgtt ataacatata gtcaaaagga gatatttatg gaagtaaaaa taagagatat
    30721 tgatgaaaaa acgattcaaa gaattgaccg aagatgtatt gaattaagtg aacgtactgg
    30781 gactaaatgg agccgaaatc gatacctaaa agttttgatc gaaaatgatt ttgatcacgc
    30841 tcttctggat tataaaaaag atcaatttga tcggttgtta gaaaaatttg tggatattca
    30901 aacttataat acgaaaattt tagaagaata tattgcaaca aataatcaat taatcgcctt
    30961 gttaattgaa cagtaaaaat aatattgtga atttataaaa ggaggaaatc aactttggcg
    31021 aaagcaacaa gaaaacatat ttatctttat gatatgcata tcgaagtact tgatgagata
    31081 atttctactc atgacgatat atccaattat agtgaagcag ttagacattt gtgtatgatg
    31141 tatgatccca aaactgaaga aaagataagt cccagtcaaa tcaaactaaa tgcaattgga
    31201 aaagaagttt ctattttaac tgaaatggtt tcggagtttg cagatcttca tttaaaagat
    31261 acaggaatat taattggtac aaattcaact gtttatcaag aagcgaaaga gcgtgtggaa
    31321 ggcaagataa aagcaaatca aacgaagaaa tattctccta agataaagaa aacaataagc
    31381 gaatcagtga ctacaaaaag tgaagtattt gatccaacaa ggtttttaaa acgataaaaa
    31441 ttagaaggag aacgtgatga gtaaatcacc tgcagtcatt ctcacttgtc aatttacctt
    31501 gcccaataaa aaatcttttt caacatacat tgattatatg acaagagaaa aagcgctgga
    31561 agaaatggaa cggcgcacgc tcgaagaaga acaggaactg caacgaatca agaatgcttt
    31621 agaggatttt tatattcctg aaggagaaac tttttcggag agtaagacgg ataaagaatt
    31681 cagtgaaacg tcgaatgaag cgcaatcgtt gatggaatat ggaatcaatt ttgacaagtt
    31741 agaggaacaa gattttacca aatatctttc ttatatgacg cgtcactatg cgttagaaca
    31801 aaaaaaggaa ctaacggaaa gggaaaaaat agaatatcaa cgtttgaatc aagcaatgga
    31861 aaaatatcaa gcagaaccac acaaaaaaac ttcaggaact cttcctggag ttttttctat
    31921 atcttcagac gaagtaaaag gaagtgatct gaaggagatt aagcagatct tccaaagagg
    31981 gcaacaaaaa ggctcaatca tttatcaaga tgttgtttct catgataatt cgtatcttga
    32041 aaaaatagga ttgtacgatc ccaaaacaga tactttagat gaagttggac taaaagctgc
    32101 tggaaggaaa atgatggaga cgttgtttga gaaagaaaaa ctgaatgata cgggatattg
    32161 gatggctacc attcaccgta acactaagca tattcatatt cattttgcag ttgttgaaaa
    32221 agaaaatacg cgtaaaccat acactgaggt cgaaaatggt gtgagttata tcgtaccgaa
    32281 aggaaagaga agacaagcaa cattagacgc aatgaagacc aattatgtgc atgaattaga
    32341 acgtttttct tatgaaaagg atcgaatgat aggcatggag aaacgaaatt tattaacgcg
    32401 gaaaagtgat ttgcgtaaca gcttaacaaa agtggtaaaa gagccagtac gttatgatcg
    32461 acacgcaatg aacttattaa atgaagtata taaaacctta cctgacaaac gttcggaatg
    32521 gaattatggg gatgaaaatc gaactaaact ttcaccatac acaagagaga aactcgacga
    32581 gttaacccaa tatgtgttaa caaaagaacc tgaataccaa gaatatatac agatttcaaa
    32641 agcattgaaa gaagaagcaa aaagtcttta tggggaaagc aagagagaat caaaggatgc
    32701 agagaaaaat gctctctttg atttaaaaaa gcgcacagga aatgccatcc taacagagct
    32761 gaagagacag gatagtcaaa taaaaccatt aatagaaagt gttgatcaga cgcaatctat
    32821 ttacaatgaa cgatcctttc gaacgcttaa acaattgcca acgaatgaat acaaatatag
    32881 ttacgaagaa ttcatgaagc gcaggaaaca attccaacaa agagttgtca cgcagcggag
    32941 tttgcgtcaa ttaaatcgaa cgattaatgc ccatgaagaa aaataccacg caagtaaagc
    33001 atacgaagag caacaacgaa gaatccaaat ggaacaagaa agacagtaaa gacagtaaag
    33061 acagtaggag gaatagcaat gaaacaatat ttagatcaat ggaaagttat agaaggatca
    33121 ttaagagaag aacgaatcga acaacttccc gattgtttag aaaaagagca tttgtttcag
    33181 ataagagaaa tgttgataaa cgaacagttt gatccaaatc aatttttagt cgttgagtac
    33241 ccagctactg gtgtgtactg ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa
    33301 gaatatgagg gcaagttagc gccttattat actacttggg aaatgaatga agaggggatc
    33361 aataacttcc cgtgtaaaag cattgaagag tctatttcac tcacagaatg ttagttgtta
    33421 agtgaataaa agaagtaaaa gagtcctgaa cgtgattgtc caggactctt ttttttcact
    33481 gaattgtttt ccgccaattc attgagtggt gcgatttccg tctacattat atcaaaagaa
    33541 aggaggaaga agaaatggct tattcaacga ttggagttgt tgttgaaaaa agcagagaca
    33601 atcttgtttt tgtgacagaa attcaaacag gcagagcttt tgtagtcaca gacaaagcgg
    33661 caaaagcata tcaaagtggc gatattttaa cgttaaatct gactacgaag acgttcgtcg
    33721 atgcggcaga agattatcca tttgtttagt ctaatggtga taagtcaaga tgaaatttaa
    33781 gattttcttt attgagtaat atgttttgaa gcgcacgaaa ctagacccga tcaaaatcaa
    33841 atttttttcg ggttgattca aggatttgcg tcaataacgg attgttattt ctctatcaca
    33901 ctccttggtg gcgtatagag ttggtgttta cgtagtagcg tatccaccag acgcgtaaat
    33961 tttcttgcgg ttaggacgag tgcacgtttg tgttgatgct ttggaacctc cttatattta
    34021 ctttgataaa aagctttgta ttcaggaaga taatttttta cagagttggc agcttcaact
    34081 aagtagtatc taaaatatcg atttccttgt tttgtcagag gtgtattttg cgactgatag
    34141 tttccagatt ggttcacttt ccaacttaat ccagcgtatt ttgccaattt tgtctgatct
    34201 tcaaatcttt cgatttgacc aatttcagcg attaaaccgg cagcatagac tttaccaacg
    34261 cctggaatac tggttaagca ttgatattct ggaatgacaa caattacttg ttcgatggct
    34321 ttatctaatt ctttgatatt tttttcaagt gctcgtattt ctcgtacgag aacacttaaa
    34381 acaacattaa ttgattcttg tgcaagagca cctaggcggt aactcatggt aattgcacgt
    34441 tgaattgctt tagcgatttt ttctggttgc ttgaaacggc cttttccttt ttcttgaagt
    34501 aaatcacaga aagcttctaa aggaagctca gcaagttcat ccaatgtgaa atcttccgtc
    34561 atgagtgaaa tgagggttgc actaaaaaga ctcgtcgatt cgtctcgcat ttctcgagca
    34621 agcgtattac atttgtacgt tagattttca agaaaatgtt gctttgtacg aatcaattgc
    34681 ttaataagtt gatagcgagt acgtgttaaa cgctgtaagg ccatgtattt ctcttctttg
    34741 ataggggaag ttgtgaatcg ttgaatacgc aagaaatcag cgattcttaa tgcatcgttt
    34801 cgatcagttt tattctcatc aaacatgcga ctaaattgtt tcacccgaaa aggattttca
    34861 atcgttacaa gtgtgttgag cttctttagt ttctcatctt catgaaaaaa catggaagga
    34921 tggaagctgt acatggacgt tgactccatg ccaatcacaa
//
LOCUS       DAGCXG010000020        34767 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGCXG010000020 DAGCXG010000000
VERSION     DAGCXG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.021"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            160..840
                     /locus_tag="MB022_001002"
     CDS             160..840
                     /locus_tag="MB022_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4466014.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="MB022_001003"
     CDS             930..1964
                     /locus_tag="MB022_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBT4466015.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="MB022_001004"
     CDS             1978..2313
                     /locus_tag="MB022_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466016.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="MB022_001005"
     CDS             2297..4777
                     /locus_tag="MB022_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466017.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="MB022_001006"
     CDS             4833..6392
                     /locus_tag="MB022_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466018.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="MB022_001007"
     CDS             6389..7753
                     /locus_tag="MB022_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4466019.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="MB022_001008"
     CDS             7924..8994
                     /locus_tag="MB022_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466020.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="MB022_001009"
     CDS             9009..9677
                     /locus_tag="MB022_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466021.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="MB022_001010"
     CDS             9697..10275
                     /locus_tag="MB022_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4466022.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="MB022_001011"
     CDS             10676..11587
                     /locus_tag="MB022_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4466023.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="MB022_001012"
     CDS             complement(11965..12882)
                     /locus_tag="MB022_001012"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBT4466024.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="MB022_001013"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="MB022_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBT4466025.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="MB022_001014"
     CDS             13579..14127
                     /locus_tag="MB022_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBT4466026.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="MB022_001015"
     CDS             14242..16602
                     /locus_tag="MB022_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4466027.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="MB022_001016"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="MB022_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBT4466028.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="MB022_001017"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="MB022_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBT4466029.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANGTKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="MB022_001018"
     CDS             19108..20007
                     /locus_tag="MB022_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466030.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="MB022_001019"
     CDS             20000..21262
                     /locus_tag="MB022_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466031.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="MB022_001020"
     CDS             21423..23321
                     /locus_tag="MB022_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBT4466032.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="MB022_001021"
     CDS             complement(23651..24370)
                     /locus_tag="MB022_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBT4466033.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="MB022_001022"
     CDS             24707..25039
                     /locus_tag="MB022_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466034.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="MB022_001023"
     CDS             25026..27029
                     /locus_tag="MB022_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBT4466035.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="MB022_001024"
     CDS             27048..27518
                     /locus_tag="MB022_001024"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBT4466036.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="MB022_001025"
     CDS             27539..28123
                     /locus_tag="MB022_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBT4466037.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="MB022_001026"
     CDS             28138..29124
                     /locus_tag="MB022_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBT4466038.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="MB022_001027"
     CDS             29114..29425
                     /locus_tag="MB022_001027"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBT4466039.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="MB022_001028"
     CDS             29447..31228
                     /locus_tag="MB022_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBT4466040.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="MB022_001029"
     CDS             31221..32597
                     /locus_tag="MB022_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBT4466041.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="MB022_001030"
     CDS             32604..33239
                     /locus_tag="MB022_001030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBT4466042.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="MB022_001031"
     CDS             33269..34624
                     /locus_tag="MB022_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBT4466043.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        11489 a         5987 c         7456 g         9835 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgggacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctcttt
//
LOCUS       DAGCXG010000021        34489 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGCXG010000021 DAGCXG010000000
VERSION     DAGCXG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.022"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            212..604
                     /locus_tag="MB022_001032"
     CDS             212..604
                     /locus_tag="MB022_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4466044.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="MB022_001033"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="MB022_001033"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBT4466045.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="MB022_001034"
     CDS             3165..4775
                     /locus_tag="MB022_001034"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBT4466046.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="MB022_001035"
     CDS             5067..5936
                     /locus_tag="MB022_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBT4466047.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="MB022_001036"
     CDS             6101..7372
                     /locus_tag="MB022_001036"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4466048.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="MB022_001037"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="MB022_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBT4466049.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="MB022_001038"
     CDS             8763..9026
                     /locus_tag="MB022_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBT4466050.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="MB022_001039"
     CDS             9471..9920
                     /locus_tag="MB022_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4466051.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="MB022_001040"
     CDS             9977..11395
                     /locus_tag="MB022_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4466052.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="MB022_001041"
     CDS             11395..12255
                     /locus_tag="MB022_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBT4466053.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="MB022_001042"
     CDS             12429..13202
                     /locus_tag="MB022_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4466054.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="MB022_001043"
     CDS             13349..13714
                     /locus_tag="MB022_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBT4466055.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="MB022_001044"
     CDS             complement(13862..15070)
                     /locus_tag="MB022_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4466056.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="MB022_001045"
     CDS             complement(15089..15367)
                     /locus_tag="MB022_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466057.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="MB022_001046"
     CDS             15521..16216
                     /locus_tag="MB022_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4466058.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="MB022_001047"
     CDS             complement(16658..17455)
                     /locus_tag="MB022_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBT4466059.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="MB022_001048"
     CDS             complement(17452..18636)
                     /locus_tag="MB022_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4466060.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="MB022_001049"
     CDS             complement(18661..19908)
                     /locus_tag="MB022_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4466061.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="MB022_001050"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="MB022_001050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBT4466062.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="MB022_001051"
     CDS             21888..22202
                     /locus_tag="MB022_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBT4466063.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="MB022_001052"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="MB022_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBT4466064.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="MB022_001053"
     CDS             22887..23531
                     /locus_tag="MB022_001053"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBT4466065.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="MB022_001054"
     CDS             23546..23875
                     /locus_tag="MB022_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBT4466066.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="MB022_001055"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="MB022_001055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBT4466067.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="MB022_001056"
     CDS             24863..25687
                     /locus_tag="MB022_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBT4466068.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="MB022_001057"
     CDS             25680..26027
                     /locus_tag="MB022_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBT4466069.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="MB022_001058"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="MB022_001058"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBT4466070.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="MB022_001059"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="MB022_001059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBT4466071.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="MB022_001060"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="MB022_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBT4466072.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="MB022_001061"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="MB022_001061"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBT4466073.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="MB022_001062"
     CDS             31657..32451
                     /locus_tag="MB022_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466074.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="MB022_001063"
     CDS             32441..34483
                     /locus_tag="MB022_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466075.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11081 a         6118 c         7246 g        10044 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccggcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatag tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacg
//
LOCUS       DAGCXG010000022        34009 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGCXG010000022 DAGCXG010000000
VERSION     DAGCXG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.023"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB022_001064"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB022_001064"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HBT4466076.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB022_001065"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB022_001065"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HBT4466077.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB022_001066"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB022_001066"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HBT4466078.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="MB022_001067"
     CDS             complement(4028..5044)
                     /locus_tag="MB022_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4466079.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="MB022_001068"
     CDS             complement(5146..5343)
                     /locus_tag="MB022_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466080.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="MB022_001069"
                     /pseudo
     CDS             complement(5315..6634)
                     /locus_tag="MB022_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7375..8355)
                     /locus_tag="MB022_001070"
     CDS             complement(7375..8355)
                     /locus_tag="MB022_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4466081.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="MB022_001071"
     CDS             complement(8368..9246)
                     /locus_tag="MB022_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4466082.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="MB022_001072"
     CDS             complement(9262..10062)
                     /locus_tag="MB022_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBT4466083.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(10337..10486)
                     /locus_tag="MB022_001073"
     CDS             complement(10337..10486)
                     /locus_tag="MB022_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466084.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            11454..11780
                     /locus_tag="MB022_001074"
     CDS             11454..11780
                     /locus_tag="MB022_001074"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4466085.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11761..12696
                     /locus_tag="MB022_001075"
     CDS             11761..12696
                     /locus_tag="MB022_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4466086.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13325..13576
                     /locus_tag="MB022_001076"
     CDS             13325..13576
                     /locus_tag="MB022_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBT4466087.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="MB022_001077"
     CDS             14118..14276
                     /locus_tag="MB022_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466088.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="MB022_001078"
     CDS             14348..15574
                     /locus_tag="MB022_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4466089.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="MB022_001079"
     CDS             complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="MB022_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4466090.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="MB022_001080"
     CDS             complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="MB022_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBT4466091.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(17033..17500)
                     /locus_tag="MB022_001081"
     CDS             complement(17033..17500)
                     /locus_tag="MB022_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBT4466092.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(17620..19158)
                     /locus_tag="MB022_001082"
     CDS             complement(17620..19158)
                     /locus_tag="MB022_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBT4466093.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            19238..19924
                     /locus_tag="MB022_001083"
     CDS             19238..19924
                     /locus_tag="MB022_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBT4466094.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="MB022_001084"
     CDS             complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="MB022_001084"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4466095.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="MB022_001085"
     CDS             complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="MB022_001085"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4466096.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(21410..22540)
                     /locus_tag="MB022_001086"
     CDS             complement(21410..22540)
                     /locus_tag="MB022_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4466097.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(22540..24723)
                     /locus_tag="MB022_001087"
     CDS             complement(22540..24723)
                     /locus_tag="MB022_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4466098.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(24720..25745)
                     /locus_tag="MB022_001088"
     CDS             complement(24720..25745)
                     /locus_tag="MB022_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4466099.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(25790..26617)
                     /locus_tag="MB022_001089"
     CDS             complement(25790..26617)
                     /locus_tag="MB022_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4466100.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(26614..27681)
                     /locus_tag="MB022_001090"
     CDS             complement(26614..27681)
                     /locus_tag="MB022_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBT4466101.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(27707..28543)
                     /locus_tag="MB022_001091"
     CDS             complement(27707..28543)
                     /locus_tag="MB022_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4466102.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(28540..29409)
                     /locus_tag="MB022_001092"
     CDS             complement(28540..29409)
                     /locus_tag="MB022_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466103.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(29479..30762)
                     /locus_tag="MB022_001093"
     CDS             complement(29479..30762)
                     /locus_tag="MB022_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBT4466104.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(30776..32461)
                     /locus_tag="MB022_001094"
     CDS             complement(30776..32461)
                     /locus_tag="MB022_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBT4466105.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            32736..33761
                     /locus_tag="MB022_001095"
     CDS             32736..33761
                     /locus_tag="MB022_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4466106.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9694 a         7314 c         5999 g        11002 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac actctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcattt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt
    15721 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
    15781 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    15841 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    15901 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    15961 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    16021 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    16081 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    16141 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    16201 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    16261 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    16321 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    16381 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    16441 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    16501 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    16561 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    16621 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
    16681 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
    16741 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
    16801 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
    16861 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
    16921 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
    16981 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
    17041 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
    17101 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
    17161 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
    17221 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
    17281 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
    17341 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
    17401 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
    17461 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
    17521 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
    17581 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
    17641 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
    17701 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
    17761 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
    17821 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
    17881 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
    17941 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
    18001 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
    18061 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
    18121 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
    18181 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
    18241 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
    18301 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
    18361 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
    18421 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
    18481 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
    18541 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
    18601 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
    18661 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
    18721 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
    18781 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
    18841 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
    18901 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
    18961 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
    19021 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
    19081 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
    19141 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
    19201 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
    19261 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
    19321 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
    19381 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
    19441 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
    19501 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
    19561 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
    19621 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
    19681 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
    19741 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
    19801 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
    19861 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
    19921 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
    19981 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
    20041 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
    20101 tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
    20161 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
    20221 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
    20281 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
    20341 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
    20401 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
    20461 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
    20521 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
    20581 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
    20641 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
    20701 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
    20761 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
    20821 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
    20881 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
    20941 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca
    21001 ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg
    21061 cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt
    21121 gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt
    21181 ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat
    21241 gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc
    21301 tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga
    21361 gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac
    21421 ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat
    21481 tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa
    21541 cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag
    21601 ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta
    21661 gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt
    21721 tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa
    21781 atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt
    21841 gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt
    21901 ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat
    21961 tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt
    22021 tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt
    22081 gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac
    22141 ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt
    22201 ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca
    22261 atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt
    22321 ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt
    22381 ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag
    22441 cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca
    22501 gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa
    22561 gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt
    22621 cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt
    22681 ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag
    22741 agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc
    22801 taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag
    22861 gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat
    22921 ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa
    22981 attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag
    23041 atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat
    23101 agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata
    23161 cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa
    23221 aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc
    23281 cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc
    23341 atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag
    23401 ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc
    23461 ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc
    23521 tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt
    23581 gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg
    23641 ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt
    23701 ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca
    23761 gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg
    23821 atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc
    23881 gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga
    23941 catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt
    24001 cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa
    24061 ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg
    24121 gaatacacct gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt
    24181 ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg
    24241 ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc
    24301 ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc
    24361 aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg
    24421 tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc
    24481 aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac
    24541 taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct
    24601 tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat
    24661 ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt
    24721 cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg
    24781 atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc
    24841 acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa
    24901 accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga
    24961 tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc
    25021 agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg
    25081 aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg
    25141 aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca
    25201 agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac
    25261 gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc
    25321 ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat
    25381 ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca
    25441 gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat
    25501 acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca
    25561 ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct
    25621 tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt
    25681 tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc
    25741 ttcatttatt tctccctcct ttgcttattt acttattgtt attctttctc tattctttct
    25801 ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct
    25861 gataaatttt cacttcattt tctcctcgca aacgacattc atataccata taaccttcat
    25921 actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt
    25981 ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg
    26041 cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat
    26101 gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt
    26161 cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat
    26221 ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa
    26281 ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc
    26341 ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc
    26401 gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga
    26461 tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt
    26521 cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc
    26581 ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac
    26641 gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct
    26701 ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa
    26761 taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg
    26821 ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag
    26881 attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc
    26941 gatccctctt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc
    27001 tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc
    27061 tagcgtgtaa tcggcaccgt atttttctgc taattctcta cgctgcgcta tgggatcgat
    27121 cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc
    27181 gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc
    27241 taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag
    27301 tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata
    27361 agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt
    27421 accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc
    27481 ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa
    27541 tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg
    27601 tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc
    27661 taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc
    27721 cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg
    27781 gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga
    27841 ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag
    27901 caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag
    27961 aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca
    28021 ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg
    28081 cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac
    28141 ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa
    28201 cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt
    28261 atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac
    28321 tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt
    28381 caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct
    28441 tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa
    28501 tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt
    28561 tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc
    28621 ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac
    28681 tttattcgtc agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac
    28741 aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca
    28801 agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat
    28861 agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat
    28921 aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct
    28981 atctactaac agatcattga cgattccata actattattg aacatatatt tccaagcaaa
    29041 aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct
    29101 aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc
    29161 tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact
    29221 taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg
    29281 catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa
    29341 taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg
    29401 acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac
    29461 cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac
    29521 atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat
    29581 atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa
    29641 agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta
    29701 accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac
    29761 ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt
    29821 ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg
    29881 atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga
    29941 aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt
    30001 gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga
    30061 caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt
    30121 tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt
    30181 aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa
    30241 aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata
    30301 ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga
    30361 acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt
    30421 ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact
    30481 gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa
    30541 cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc
    30601 agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg
    30661 aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa
    30721 tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt
    30781 ttattatttt cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc
    30841 aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg
    30901 ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa
    30961 aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt
    31021 tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg
    31081 gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc
    31141 gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa
    31201 tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt
    31261 tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta
    31321 tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg gtaccctctt
    31381 tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat
    31441 tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga
    31501 aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa
    31561 gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa
    31621 aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag
    31681 ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta
    31741 tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag
    31801 cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt
    31861 taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt
    31921 ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat
    31981 ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt
    32041 cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg
    32101 tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat
    32161 aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa
    32221 ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt
    32281 gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat
    32341 gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag
    32401 aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca
    32461 tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg
    32521 cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa
    32581 gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag
    32641 ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat
    32701 tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg
    32761 cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata
    32821 tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga
    32881 gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc
    32941 gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg
    33001 ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag
    33061 agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa
    33121 gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg
    33181 taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg gataatttcc
    33241 aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc
    33301 tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct
    33361 tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag
    33421 aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt
    33481 tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc
    33541 agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt
    33601 cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac
    33661 ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg
    33721 agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac
    33781 agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac
    33841 cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat agctgtggat
    33901 aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca
    33961 cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctt
//
LOCUS       DAGCXG010000023        29148 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGCXG010000023 DAGCXG010000000
VERSION     DAGCXG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.024"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            268..1053
                     /locus_tag="MB022_001096"
     CDS             268..1053
                     /locus_tag="MB022_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4466107.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPAQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1066..1917
                     /locus_tag="MB022_001097"
     CDS             1066..1917
                     /locus_tag="MB022_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBT4466108.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2041..2493)
                     /locus_tag="MB022_001098"
     CDS             complement(2041..2493)
                     /locus_tag="MB022_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466109.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(2958..3326)
                     /locus_tag="MB022_001099"
     CDS             complement(2958..3326)
                     /locus_tag="MB022_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBT4466110.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3476..3958
                     /locus_tag="MB022_001100"
     CDS             3476..3958
                     /locus_tag="MB022_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBT4466111.1"
                     /translation="MIDLTKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            3995..5005
                     /locus_tag="MB022_001101"
     CDS             3995..5005
                     /locus_tag="MB022_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBT4466112.1"
                     /translation="MISYPLLERNATIGVTSPSSGVSEEHHSLLESALKRMEEKSFHV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTRKGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5120..5833)
                     /locus_tag="MB022_001102"
     CDS             complement(5120..5833)
                     /locus_tag="MB022_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466113.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(6023..6613)
                     /locus_tag="MB022_001103"
     CDS             complement(6023..6613)
                     /locus_tag="MB022_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBT4466114.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7096..7323
                     /locus_tag="MB022_001104"
     CDS             7096..7323
                     /locus_tag="MB022_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBT4466115.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7339..8505
                     /locus_tag="MB022_001105"
     CDS             7339..8505
                     /locus_tag="MB022_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBT4466116.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8740..9783
                     /gene="hrcA"
                     /locus_tag="MB022_001106"
     CDS             8740..9783
                     /gene="hrcA"
                     /locus_tag="MB022_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBT4466117.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9877..10440
                     /gene="grpE"
                     /locus_tag="MB022_001107"
     CDS             9877..10440
                     /gene="grpE"
                     /locus_tag="MB022_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBT4466118.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10491..12320
                     /gene="dnaK"
                     /locus_tag="MB022_001108"
     CDS             10491..12320
                     /gene="dnaK"
                     /locus_tag="MB022_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBT4466119.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12471..13637
                     /gene="dnaJ"
                     /locus_tag="MB022_001109"
     CDS             12471..13637
                     /gene="dnaJ"
                     /locus_tag="MB022_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBT4466120.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVIFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(14008..14745)
                     /locus_tag="MB022_001110"
     CDS             complement(14008..14745)
                     /locus_tag="MB022_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4466121.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14747..16249)
                     /locus_tag="MB022_001111"
     CDS             complement(14747..16249)
                     /locus_tag="MB022_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBT4466122.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            16508..18502
                     /gene="uvrB"
                     /locus_tag="MB022_001112"
     CDS             16508..18502
                     /gene="uvrB"
                     /locus_tag="MB022_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBT4466123.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQCL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            18512..21328
                     /gene="uvrA"
                     /locus_tag="MB022_001113"
     CDS             18512..21328
                     /gene="uvrA"
                     /locus_tag="MB022_001113"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBT4466124.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNGLSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            21591..22856
                     /locus_tag="MB022_001114"
     CDS             21591..22856
                     /locus_tag="MB022_001114"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBT4466125.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            22860..23591
                     /locus_tag="MB022_001115"
     CDS             22860..23591
                     /locus_tag="MB022_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBT4466126.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            23726..24610
                     /gene="rapZ"
                     /locus_tag="MB022_001116"
     CDS             23726..24610
                     /gene="rapZ"
                     /locus_tag="MB022_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBT4466127.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            24607..25605
                     /locus_tag="MB022_001117"
     CDS             24607..25605
                     /locus_tag="MB022_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBT4466128.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            25639..26574
                     /gene="whiA"
                     /locus_tag="MB022_001118"
     CDS             25639..26574
                     /gene="whiA"
                     /locus_tag="MB022_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBT4466129.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            27142..27798
                     /locus_tag="MB022_001119"
     CDS             27142..27798
                     /locus_tag="MB022_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466130.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWITPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGIIRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            27977..28534
                     /locus_tag="MB022_001120"
     CDS             27977..28534
                     /locus_tag="MB022_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466131.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRISYVPESANYFMGDVQKEREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLVSWEHDPPIENRFE"
BASE COUNT         9671 a         4888 c         6190 g         8399 t
ORIGIN      
        1 tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag aagtaatttt
       61 tgtaaattac ttcttatttc ttgaagctgg ccacttctgt cacaacctct ttctttatag
      121 aaataaaagg acaatagcaa tttatgaaaa cgattgctag atcgtatcat ttgctttaga
      181 ctatatatct tggtgtacac tcactttaag gcggaaataa aagatatccg taataacagg
      241 tttaggtata aaaaaggagt gggtactatg tataaggaat tagaaaacaa agtcgcagta
      301 gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg gaaagaaaaa
      361 atgaatgtag tcgtcaacta taattcggat aaagaaggag cagaaaaagc agtcaaagca
      421 atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc tgaagaagga
      481 atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt ttgggttaat
      541 aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga ttgggaaaga
      601 gtattaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt aaattacttt
      661 acagagaatg ataaaaaagg aaatatcatc aatatgtctt ctgttcatga acaaatccca
      721 tggccaacgt ttgctcatta cgctgcttca aaaggcggag taaaactatt caccgaaacg
      781 gtagcaatgg agtacgcacc aaagggaatc agaatcaata gtataggacc tggtgctatc
      841 cgtacaccta ttaatgcggc aaaatttgat gatcctgcac aaaaagaact cttggaaagt
      901 atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc tgcatggttg
      961 gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg tgggatgact
     1021 ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg tttattgatt
     1081 gcattgatcc cggcagttgg gtgggggatc cagccactga ttttgaaaaa gattggtggc
     1141 agaccaacaa atgagatatt aggaactggt attggagccg tgctcgttgg cttacttgtt
     1201 caattattcg cttctccagc tggtatttca gtgactacat ttttgattag tttactttct
     1261 ggtgcattct gggtcatcgg acaaacagga cagtatacat ctttcaactt gattggtgtt
     1321 tctaaaacga tgcctatctc gactgcatta cagttagtcg gaacctcatt gattggtgtc
     1381 ttcgcttttg gagaatggtc tggaagtatg ggaaaaatca ttggtgcagc agcaatcatt
     1441 cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa aaaaggcggt
     1501 atcacaaaag gaatcagtct attagcatca acaagtattg gttattgggt ttacagtgca
     1561 ttgcccaaac tagtggatgc aagcggactt tccattttct ttccgcaaat gatcggtgtc
     1621 tttttagggg cagtgattta tgtgattttc cgaaaaccaa ccgcattttc tgaagacaag
     1681 agttggaaag caacaatagt tggagccatc ttcagtgtgt cagcattggc ttatattttt
     1741 tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa cgtagttatc
     1801 tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga aaaaaaatta
     1861 acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt tatttgagga
     1921 gaaagatggt aaaatctcat ttattaaaaa aatatttgtt tgaaccaaac agtaagtact
     1981 tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct ttttaattgt
     2041 ttaagataca agggaaatat ctgtctgcat caataatttt tcattgatcc attgactaaa
     2101 gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt ctttataaag
     2161 atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt actggactaa
     2221 ttgttctcgg aattgattga ttgctgtaaa atcacgatac tgaaggttga aacgcatcgt
     2281 gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg cttgatctcc
     2341 gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa cctttgttcc
     2401 ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac ctaattgttc
     2461 ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt gaatggttct
     2521 actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt taattaaggt
     2581 cattgtaaaa atcaagtcag aaaattctaa cttttctttc tctaaagaaa ttcgttctct
     2641 cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta tgaaagatca
     2701 ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt gctgaaaaaa
     2761 taattttact aagtggaaaa ttcaaagcca ttcaatcagt gatttttttc agcacaagaa
     2821 agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag ggatgataaa
     2881 aaggatagga ttgaacaaaa cctgtataag ctgatgaata gtttcctcgc cagtaaaagg
     2941 tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc ttcaatatta
     3001 aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg aactttgatg
     3061 acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc acgaatcgct
     3121 gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat aataaaggta
     3181 ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg gggatacgtt
     3241 tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc tttgatctga
     3301 ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt ttgatttatt
     3361 ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc
     3421 gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca taaaaatgat
     3481 tgacttgaca aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc
     3541 catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga
     3601 gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac
     3661 tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt
     3721 ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg
     3781 ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt
     3841 ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat
     3901 catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc
     3961 aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag
     4021 aaatgcaacg attggggtta cttcaccatc ctcaggcgta tcagaggaac accattcgtt
     4081 actcgaatca gctttaaaga gaatggaaga aaaatctttc cacgtagtta ctggaaagac
     4141 gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa
     4201 tcaaatgatg caagatgaaa atatccaaat gatctttccg ccttggggag gagaattatt
     4261 gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta
     4321 ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca
     4381 tggaaccaac cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga
     4441 aagtgtactg aaaacaagaa aaggagaatc gattctacag caaccttctg aaaagtatca
     4501 aaaagagtgg ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa
     4561 ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag gtggctgtat
     4621 tgatacgatc agtcatttaa tcggtacccc ttatggagat gtccaatctt ttaaaagaaa
     4681 gtataatgat ggaaagccga tcttgtggtt tctagaaaat tgtgagctat cgtcagttga
     4741 tttaagaaga tcacttgttc agatgaaact ggcaggatgg tttgatggca gctcggggat
     4801 catgttcggt agaagtgcag cagaggattt gttagattat aaaatgaagg atgtatacga
     4861 agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc
     4921 cccacaactg acattgatca atggtgccta tgcagaagta gcggtagaag atagaaaagc
     4981 gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagttt atcaaagacc
     5041 aacaaatcta aatagaaaag aaaatgaaag aagctgacag tattttgcca gcttcttttt
     5101 gtatgatcta ttagaaattt taattatcca taactatcac attgatcagc tgtttcacat
     5161 catttggatt acctgtagga atagctacta cagatgcatt atcaataatt tcttgagttg
     5221 tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa
     5281 ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac
     5341 tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct gcatcataag
     5401 gggcgatagg tgttcctgca ggataatgaa taacatgtag ttcgaaatca cccgatgtat
     5461 ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt tgtagatcag
     5521 aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg tctattgttg
     5581 aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga
     5641 ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt
     5701 tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat ggatcactat
     5761 tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat aaaataaaac
     5821 ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc ataatcctga
     5881 atttttgaat ccgtaaaacc ttgtctataa atataaatag agtgtatctt ctttgataaa
     5941 tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact attgataagg
     6001 agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat
     6061 agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga
     6121 ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat
     6181 tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt
     6241 ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag
     6301 ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat
     6361 cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg atcataataa
     6421 aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc
     6481 caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt
     6541 ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa
     6601 tcgcagtttt cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca
     6661 tacagacatt cattaaattt ggatagcgac agcgaaaaca acaattctta agttttaaac
     6721 agagtataca gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat
     6781 ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac caattcccac
     6841 ttgcttgagt acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaaaaaaaat
     6901 aaatcatatc gggcactcag acatgaaaac aaccctagag atatataact aggtaaccag
     6961 tttaatgaaa gaaaaaatca tccaagaagt cgattcttgg attttttagt ggatttgttt
     7021 gccaaatacg tttagtatgc gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca
     7081 cttaaggaaa aatctgtgaa aatagctttt catatttttg cagattttga cttaatgccg
     7141 aaggtcagct ttgcgattac cgtttatcga gaggctgtaa aagcaccgtg tagctccgat
     7201 gacaataagc aagcattttt agagctaggt ttccttgatt ctaaatatgc ggcttatttc
     7261 aatgagttgg tgcttgaacg cgattttaag aaggtcgaaa acaggcacga cttttcgacg
     7321 taaggagaaa gaatttcaat gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg
     7381 tacattcata tcccattttg cgagcatatc tgctattact gtgactttaa taaagttttt
     7441 ttagaagggc agccagtaga tgagtatgtg gaaatgctgc tgaatgaaat gagattaatg
     7501 ctggaaagtc atcctacagc gaatttggaa acactttatg taggaggcgg aacaccaact
     7561 tctttatctg ctaaacaact tgatcgacta ttatcaggtg ctagagagat cctgcctttt
     7621 caaaatggta atgagtttac tgtagaagcg aatcctggtg atttgacccg tgaaaagctt
     7681 caagtgatga aaaactatgg cgtcaatcgt ttatctatgg gagtgcagac atttgacaat
     7741 cgcttattga aaaaaattgg acgtaaacat actgcagaag atgtgtatca aactatgcaa
     7801 tttttagaag cagaaaattt taccaatgta agcatcgatt tgatttatgc actaccggga
     7861 caaacactag aaggatatcg ggatactttg gaaaaagctt tggctttgga tctgcctcac
     7921 tattctttgt actcgttgat cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa
     7981 ggaagactcg aactgcctga tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa
     8041 gcaatggaaa agcatggacg tcaccaatat gaaataagta actttgggct ctccggacat
     8101 gaatctcaac ataacctcat gtactggaac aatgaccact attttgggtt tggagctggt
     8161 gctagcggtt atcttggaga tattcgttac cgcaacaaag gaccaatcca acactattta
     8221 cgcccattac gagcaaatga acgcccggta ttggaagaag aaaaattgac acgaaaaaat
     8281 caaatagaag aagaaatgtt cttaggacta agaaaaaaaa cagggattac taaaaaacat
     8341 ttctatgatc gctatcaaaa aacaattgaa tcggtgtacg gtgatgtgct aagggaatta
     8401 gagcaagaag ggttacttgt taatgaagaa gaccgtgttt atttgacacc aagagggact
     8461 tttttgggga acaatgtgtt tgagcgcttt ttatttgaaa aatagtttga agaatatttg
     8521 ttttttatcg aatcttgaaa agaaaaagtt tagaaaggtt gacaatgagg tattgaagta
     8581 ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt tagagtgcta
     8641 aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat aagtggatta
     8701 gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaat
     8761 gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt tggttccaaa
     8821 acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga tatgaaagca
     8881 ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat tccatctgct
     8941 ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat
     9001 ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga gattattaag
     9061 caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt gggacctgaa
     9121 ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg acagattatt
     9181 gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct tcccgaatat
     9241 atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct agtcggagat
     9301 cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct tcataaatat
     9361 ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca tgtttttgaa
     9421 gaaaaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg tcaagatctt
     9481 gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac acaaatgatc
     9541 actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa tgatttattg
     9601 cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag aggtacaata
     9661 gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt cgatgtttat
     9721 cgaagagaac tagcagctaa acttgctgat tactaccgct cgcttgactt gtcaaactcg
     9781 taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt aagagcaatc
     9841 aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca agaagaacta
     9901 gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat
     9961 tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa agctgagtta
    10021 gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga
    10081 ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc caaaaaatta
    10141 cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg
    10201 gcaggtttga aaaaaggcgt ggaaatggtt ttggaaagtt tgcgtaacgc actggaagaa
    10261 gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct tcatcaagca
    10321 gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga agtactacaa
    10381 gaaggctaca aattacatga tcgcgtgtta agaccaacta tggtcattgt tgcacaataa
    10441 ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt atgagtaaaa
    10501 ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa ggaaacgaag
    10561 caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa
    10621 atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga
    10681 tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta gaaggtaaat
    10741 catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga ttcgcagaag
    10801 actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac tttaatgatg
    10861 cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta gaacgtatcg
    10921 tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat cgcgacgaaa
    10981 aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt gaactaggag
    11041 acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg gacgactttg
    11101 ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc attgacttgt
    11161 ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt
    11221 tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga gaatctggac
    11281 cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca gctgacttag
    11341 tagaacgtac aaaagtacca gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag
    11401 aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt gtagaagcag
    11461 tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta
    11521 tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg
    11581 acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact aaattgattg
    11641 accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg gcagataatc
    11701 aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct gacaacaaaa
    11761 ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt ccacaaatcg
    11821 aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta
    11881 cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac gaagaaatcg
    11941 aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt aaagaggaag
    12001 ttgacctacg taatgatgta gatgcattac tattctctgt ggacaaaaca ttgaaagaat
    12061 tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgt gacgaattaa
    12121 aagccgctgt tgaagcaaac aacatcgagg aaatgaaaac taaacgcgat gcattgaacg
    12181 aaatcgttca aaacttgact gttaaactat acgaacaagc tgcacaacaa caagctcaag
    12241 aaaatccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat tttgaagaag
    12301 tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa agttgctact
    12361 ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa caaaatatga
    12421 tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct atggcaacaa
    12481 aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat gaaataaaga
    12541 aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa cctgatgcgg
    12601 aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg caaaaacgcg
    12661 ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct ggtggagctg
    12721 gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt tttgaagata
    12781 tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag
    12841 gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa
    12901 aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac ggtgcaaaac
    12961 caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatc aatgtagaac
    13021 gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt catggcacag
    13081 ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag aagaaagcac
    13141 attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg cgtttagctg
    13201 gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg atcttccgtg
    13261 tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa
    13321 gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat ggagatgtga
    13381 aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt ggaaaaggcg
    13441 ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa ctgatcacac
    13501 cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa gctggcggca
    13561 tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat gcttttggcg
    13621 ggaaaaagcg gaagtagttt ttctagtaaa aatataaagt agacttgccc aaattttcta
    13681 tttctagtaa aaacgctaag tcattaataa ttaaaaacca gacgaacaat catacgttcg
    13741 cctggttttt ttgtagttgt catgtttgta ccaacaaaaa agccaacttt agaataagat
    13801 aatcatcata gtttctgaag gtggtatcga ctatgtactt gatattgtca atgaaccgat
    13861 ggtactttct tatgcgatgg aacaggtaga gcgattgctt gtttgtgcag gaaaacaagc
    13921 gatgcggctg atgatacgat taggtgagaa aataagaaga taaaaataca cggtcccttc
    13981 atggcgaccg tgcatttttg gtttttatta aatatttaat actttattca agaaatcttt
    14041 tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccgtctt ctaaaaagtt
    14101 tccgtcgtca atgaacatga cgcgattggc cacttcttta gcgaacccca tttcgtgcgt
    14161 aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc
    14221 gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt cagggttcat
    14281 cgctagagca cgagcaatag ccacacgctg tttttgtcca ccagaaagca tttcaggata
    14341 catatctttt ttatcttcta gaccaactgt ctgcagtaat ttgattgctt ttttctctgc
    14401 ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga
    14461 aagatgaggg aataggttaa agtgttggaa taccattccg atatgttgtc tcaccaagtt
    14521 gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg
    14581 ttcttctaaa cgattcaagc aacgcaaaaa tgtacttttt cctgaacctg aagggccaat
    14641 gatacagacg acatctcctt cgttgatgga tacattgatg tcgtttaata ctgtgttatc
    14701 gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct
    14761 tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa
    14821 tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca
    14881 gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact
    14941 gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg
    15001 tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt
    15061 tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa
    15121 caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa
    15181 agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg
    15241 tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag
    15301 ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt
    15361 gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg
    15421 aagtttctga accatccgct acatattctt taataatctt atcgtattcg cctgttcttt
    15481 ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa
    15541 cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt
    15601 gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt
    15661 ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag
    15721 attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc
    15781 ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc
    15841 cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca
    15901 ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac
    15961 cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta
    16021 catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt
    16081 cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc
    16141 ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa
    16201 gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc
    16261 taaatttcca atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc
    16321 tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaaca taacatgtat
    16381 aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg
    16441 cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg
    16501 acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga aataccaacc
    16561 ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa
    16621 agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat
    16681 tgagaaagtg aataaaccaa cactgattat tgctcataac aaaacattgg ctggtcagct
    16741 gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta
    16801 tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc
    16861 aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat tattagaaag
    16921 aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggct cgccttttga
    16981 gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagctgat
    17041 tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt
    17101 tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg
    17161 tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga
    17221 agttttggga gaaacggaac acgtagcaat cttcccggcg actcactttg tgacgaatga
    17281 tgaacatatg gagcatgccg tagcgaatat caaagcggaa ttagaacagt gtttaactgt
    17341 cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattatga
    17401 tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat
    17461 ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt
    17521 tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa atcagaggaa tgtataatgg
    17581 tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga
    17641 caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc
    17701 tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg
    17761 tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga
    17821 tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt
    17881 gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa
    17941 atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt
    18001 aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga
    18061 agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc
    18121 cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc
    18181 agacaaagta acagattcta tgcgtctagc gatggatgaa acatcaagac gccgtacgat
    18241 tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg
    18301 agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc
    18361 gtatgaagaa atgacgaaag aagaaaaaga tacgttattg atgaaattag aaaaagaaat
    18421 gaaagatgcg gcgaaagcat tggattttga aacagccgct aatgtgcgag atatgatttt
    18481 agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcacgg
    18541 tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt
    18601 cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga
    18661 agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat
    18721 ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa
    18781 aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt
    18841 acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac
    18901 cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgaac gcacaaagat
    18961 ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg
    19021 aatccaaaaa gagggatacg tccgcgttcg agtcgatggg gagatctatg atttaagtga
    19081 agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat
    19141 tgtgaaagaa gggattcgtt ctcgtctatt tgattcttta gaggcggccc tgcgattggc
    19201 agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta
    19261 tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa
    19321 tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca
    19381 agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa
    19441 tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat
    19501 cgatttagat acaccattcg aagaattacc ggaagagcat caggagatca tcttgaatgg
    19561 gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga
    19621 aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga
    19681 tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg
    19741 ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac
    19801 aaatgggttg tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca
    19861 agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga
    19921 aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga
    19981 agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat
    20041 tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt
    20101 aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat
    20161 gtgggcagca gattatctga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat
    20221 cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata
    20281 tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc
    20341 aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt
    20401 aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag
    20461 tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt
    20521 caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat
    20581 cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg
    20641 gttatttgct caaacgaatg aggcaaaaat gcggggttat aagaaagggc gttttagttt
    20701 caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat
    20761 gcactttttg cctgatgtct atgttccttg tgaagtatgt catggcaaac gatataactc
    20821 tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt
    20881 agaagatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat
    20941 tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg
    21001 tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt
    21061 ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca
    21121 tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga
    21181 tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg
    21241 aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg
    21301 tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac
    21361 ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt
    21421 agttctttct acactgattg gtatagataa aaacttccta taagttcaat ccgttcaaaa
    21481 aagaattgag caatcggagg agtttccttt gattgctttt ttctttatgg tatgatattc
    21541 cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca
    21601 gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta
    21661 tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt
    21721 tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag
    21781 accctgtatt tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta
    21841 aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac
    21901 aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt gaaagactga
    21961 tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat
    22021 tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat
    22081 tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa
    22141 gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatatta ggccggatat
    22201 acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta
    22261 atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgtcttg
    22321 gtcatccact gttagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc
    22381 cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata
    22441 caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg
    22501 aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg
    22561 ctcgttttgt cacagaagat cgagtgttca acaaaccctt tgttgaaacg acctatggaa
    22621 cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat
    22681 atgctaggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg
    22741 gtactactgt gttttatgaa aaagaccggt caatcaagcg ctggttgttg atgaaatata
    22801 tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca
    22861 tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc
    22921 gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat
    22981 tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc
    23041 caatgccgtt tctgctggat gatattgaga aacaaaattt attgcagccg aacttcattg
    23101 tgttgacttg taacaccgca cactactttt ttgaagaatt acaagcggca acggatattc
    23161 ctatcctgca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga
    23221 gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca
    23281 aaaatcttgg attcgaaaca gtgattcctg atactgttct tcaagaaaaa atcaattatc
    23341 tgatctatca tgagatcaag gaatcggatt atttgaatca agagctatat tacgaaatct
    23401 tagaagaagc ggtagagcgc ctaaactgtg aaaaagtcat cttaggttgc acagaattgt
    23461 cattgatgca tgaatttgct gaagataacc attatccagt gatcgatgcc caatctattt
    23521 tagcagatcg gacgattgaa cgagcgctag ctgaaagaag caaggcattg gatacagcat
    23581 cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct
    23641 ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg aatgatataa ttttactatg
    23701 cacaatgtga gaggagttcg taattatgac agataatttg caattagtca tcataacagg
    23761 tatgagcggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg
    23821 tatcgataat atgccgcctc gattgattcc aaaattctgg gaattaatca aagaatcagg
    23881 caaagtcaca aaaatggctt taggagtgga tttacgttct cgttccttct ttgaagaaat
    23941 ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt
    24001 agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat
    24061 ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt
    24121 gaaagcccaa gcttccgtga tcattgatac gacgacattg acaccacgac aattacgtga
    24181 aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc
    24241 atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt
    24301 gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta
    24361 tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct
    24421 ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg
    24481 ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg
    24541 gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa
    24601 ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt
    24661 ggcactggtc tgccagttat cctgaaaagt ttgcgaaatc aaggagcaga tatcacagca
    24721 gtagtgactg ttgcagatga tgggggaagc agcggtgcaa tcagagaatc gatcgctatg
    24781 gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat
    24841 gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc
    24901 ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa
    24961 ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg
    25021 accttgcatg ctgtctttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc
    25081 gaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa
    25141 gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga
    25201 agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc
    25261 aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa
    25321 catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc
    25381 gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat
    25441 gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga
    25501 gtgatttcca ccgacttttt ggaattgaaa gatggtggtg tcttccacga tggagacaaa
    25561 gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg
    25621 agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg
    25681 gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct
    25741 ttaggtattg tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc
    25801 agacgtatct attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt
    25861 aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa
    25921 gatgtgttga tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac
    25981 gagattatgg gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca
    26041 ggttcagtca ataatccgga aacgagccgt tatcatttgg aaatattttc tatttatgaa
    26101 cagcataacc aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt
    26161 gaacggcgca atggctatat ttcttattta aaaggagcag aaaaaattgc tgatttctta
    26221 acattgattg gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat
    26281 atgagaaatt cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt
    26341 gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat
    26401 gcccttcctg aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc
    26461 ttgaaagaat taggggaaat gattccctca ggtgcgatat caaaatcggg aattaatcac
    26521 cgaattcgca aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa
    26581 gaaaaaactg atacatgaag caggagatgc gaatgtatca gtttttttta aaagttatac
    26641 ttgcttttct tccgtcaaac tataattcca tctttactgg agtaacataa tttggaaagg
    26701 cttctattgt ttttaataca atcgtataaa aagcggggag acaactgaat gaagatttta
    26761 gttttgtaca taaatttttg cttaatatag aagtcctgat atagataaga tcaagtattt
    26821 atctataact ggcctgatta attaaaaaac tggaaggaac tgctcgatca tccttgagaa
    26881 ctgctcttct tacttctggt caagattctc gtggttggtg gataaggatg gttgataagt
    26941 tcattggatt agctagtcga aaacaaccag gtgtaattaa tgtagaagtt ttaacaaggg
    27001 gatggagtga tttattcagt gataatcata agttcataaa tagtaattga gatgtatggt
    27061 tggaatacgt tatacctgaa cttgaactac caagatttgg tttagaattt gagaatcgat
    27121 gtttataaag ggagggcaaa aatgcgtttt tttacaactt ttactatcat tatgattgca
    27181 gtacttttta tttttctaga tatagcaaag cgaaatacag catttttatt atatcgggtt
    27241 ttacttcgag caggtctcat aacatttata tcgatcgttg gattttttct attcacagtt
    27301 attgtattta tttggataac tccagcacca ccattgccag agattacata tggagaattt
    27361 ccttttagac ttgagtatga attgaatgaa gaattacatg tgatagaaga cacgttaata
    27421 gttgaatttg atggatttgg aatgaacgaa ggcataggga gatatcgtag atggacaagt
    27481 cgtttagcca gcggagaaga tttagtgttg ctgttagaag ttagtgataa taagcaaata
    27541 ttttattttc caggacctgc taattattac atgggagata ggttgaatgg ttataatcat
    27601 acttttccta gtgcttcttt cattgaaagg gaaagaggca ttattagaag ggatatttta
    27661 catgacaaag aattattaga acaatttggt ccgcttgatc aaaatacaat aaatgaagaa
    27721 gaattgttaa atcaatataa tataaggcta gtgaattggg aaatatctga accgattgtt
    27781 aacaattttg gtgattagaa aacaattacg ataaatagaa acaagccaaa tcatcagcaa
    27841 gtaaaagctg atgatttggc ctttcttata cgagtagtag gaattcatag cgaatattat
    27901 tacgatcagt agatattttg atattaaagg agtattacga actagtatgt gagaatgtag
    27961 aggtgatttg cgaataatga gaaatttatc aaatagaaac aaaatattaa tcattattgt
    28021 tgttatagct gtgtttcatc tgggaacgaa tgcggtttta agtagaataa tattgggtcc
    28081 taagccacca aggccagaaa ttacccgagg ggagtttgat tttagattag aatatgaagt
    28141 agatggggaa aggattgtga tagaagatac aattgttgca ttatttgatg gattttctgc
    28201 agatgcaggt agcatggcat ggtatcgcac gtggcgtcta catttagcaa gtgatagacg
    28261 atcacgaaat atcttattag atgaattaga ggatggtcga agaatatctt atgtcccaga
    28321 aagtgctaac tattttatgg gagatgtaca gaaagaaaga gagcctaatc ctaattggta
    28381 tccatttaat ggcgtaacaa tagaatatcc acgcaataaa acacctgaaa taggtgcaaa
    28441 atttataagt ggtttagaag atctgtacga taggtttgga atccgtcttg ttagttggga
    28501 acacgatcca ccaatagaaa atcgttttga ataatcgaaa tctgctaatc catttcatca
    28561 gccagtgaat gttgtaatgg aacaatttgg tttagtgttc aaaaccattg aaatactagg
    28621 atgtgatcag caaaaatgaa tctgaataaa aaagagagca actattagat gagaggagtt
    28681 aagagttgtt agctttatac gaatcaaagt ggcaaatata gatgcgtatg catttgtgtt
    28741 tgccatcttg catttggtag acgagatagg tataagctaa agaaataact tgtttaaaat
    28801 aaacgaccta gctcaacaaa cgaccatcta atcaactaac agcatacata aaagcaacta
    28861 tttttgaagt agaattgaaa tcgggataac ttaaaaagtg attgggatac tagtgagaat
    28921 caccgtacat aaatattact cgttaagggg aattgagaaa ttttgtatga aaatttaaga
    28981 attagttagt atgcgcaagt caaaaatatg aataaattat ttggtggaga taatttgctc
    29041 agcttgtcat aagaaatgag ataatctggc agatgcaagc ttttggacac catttattcg
    29101 gaataaggag gttgggacag aagcgtttaa ctccaagaaa taagaagg
//
LOCUS       DAGCXG010000024        28612 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGCXG010000024 DAGCXG010000000
VERSION     DAGCXG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.025"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            42..1183
                     /locus_tag="MB022_001121"
                     /pseudo
     CDS             42..1183
                     /locus_tag="MB022_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1309..2388)
                     /locus_tag="MB022_001122"
     CDS             complement(1309..2388)
                     /locus_tag="MB022_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4466132.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2491..2931)
                     /locus_tag="MB022_001123"
     CDS             complement(2491..2931)
                     /locus_tag="MB022_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4466133.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2988..3398)
                     /locus_tag="MB022_001124"
     CDS             complement(2988..3398)
                     /locus_tag="MB022_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4466134.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3578..4012)
                     /locus_tag="MB022_001125"
     CDS             complement(3578..4012)
                     /locus_tag="MB022_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4466135.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4112..4960)
                     /locus_tag="MB022_001126"
     CDS             complement(4112..4960)
                     /locus_tag="MB022_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4466136.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5349..7115
                     /locus_tag="MB022_001127"
     CDS             5349..7115
                     /locus_tag="MB022_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBT4466137.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7217..8476
                     /locus_tag="MB022_001128"
     CDS             7217..8476
                     /locus_tag="MB022_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4466138.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8623..9924
                     /locus_tag="MB022_001129"
     CDS             8623..9924
                     /locus_tag="MB022_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466139.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9927..10778
                     /locus_tag="MB022_001130"
     CDS             9927..10778
                     /locus_tag="MB022_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466140.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10791..11606
                     /locus_tag="MB022_001131"
     CDS             10791..11606
                     /locus_tag="MB022_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBT4466141.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11757..12923
                     /locus_tag="MB022_001132"
     CDS             11757..12923
                     /locus_tag="MB022_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4466142.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13153..13800
                     /locus_tag="MB022_001133"
     CDS             13153..13800
                     /locus_tag="MB022_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4466143.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13884..14828
                     /locus_tag="MB022_001134"
     CDS             13884..14828
                     /locus_tag="MB022_001134"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBT4466144.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14963..16414
                     /locus_tag="MB022_001135"
     CDS             14963..16414
                     /locus_tag="MB022_001135"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBT4466145.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16435..17271
                     /locus_tag="MB022_001136"
     CDS             16435..17271
                     /locus_tag="MB022_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4466146.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17271..17930
                     /locus_tag="MB022_001137"
     CDS             17271..17930
                     /locus_tag="MB022_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4466147.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18056..18745
                     /locus_tag="MB022_001138"
     CDS             18056..18745
                     /locus_tag="MB022_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466148.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18751..19707
                     /locus_tag="MB022_001139"
     CDS             18751..19707
                     /locus_tag="MB022_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBT4466149.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19751..20263
                     /locus_tag="MB022_001140"
     CDS             19751..20263
                     /locus_tag="MB022_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466150.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20519..21067)
                     /locus_tag="MB022_001141"
     CDS             complement(20519..21067)
                     /locus_tag="MB022_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4466151.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21156..22337)
                     /locus_tag="MB022_001142"
     CDS             complement(21156..22337)
                     /locus_tag="MB022_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBT4466152.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22573..24081)
                     /locus_tag="MB022_001143"
     CDS             complement(22573..24081)
                     /locus_tag="MB022_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBT4466153.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24240..24431
                     /locus_tag="MB022_001144"
     CDS             24240..24431
                     /locus_tag="MB022_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466154.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24634..25551
                     /locus_tag="MB022_001145"
     CDS             24634..25551
                     /locus_tag="MB022_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBT4466155.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25544..26617
                     /locus_tag="MB022_001146"
     CDS             25544..26617
                     /locus_tag="MB022_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466156.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26765..27283
                     /locus_tag="MB022_001147"
     CDS             26765..27283
                     /locus_tag="MB022_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466157.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27264..27656
                     /locus_tag="MB022_001148"
     CDS             27264..27656
                     /locus_tag="MB022_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBT4466158.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27732..>28612
                     /locus_tag="MB022_001149"
     CDS             27732..>28612
                     /locus_tag="MB022_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBT4466159.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDK"
BASE COUNT         9592 a         5153 c         5558 g         8309 t
ORIGIN      
        1 cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct
       61 taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag
      121 aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct
      181 ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag
      241 cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg
      301 ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta
      361 tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac
      421 cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt
      481 atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg
      541 aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc
      601 cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac
      661 cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc
      721 cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata
      781 atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc
      841 cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa
      901 ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg
      961 aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa
     1021 aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc
     1081 cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac
     1141 aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg
     1201 tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga
     1261 aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc
     1321 tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt
     1381 aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa
     1441 gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag
     1501 cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg
     1561 gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc
     1621 tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc
     1681 aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc
     1741 ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa
     1801 gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc
     1861 aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat
     1921 atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt
     1981 tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc
     2041 gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca tcgggatata
     2101 ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac
     2161 acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt
     2221 aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac
     2281 gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac
     2341 ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa
     2401 tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt
     2461 tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt
     2521 atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag
     2581 ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagc
     2641 agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt
     2701 tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt
     2761 tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg
     2821 ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga tttctttaga
     2881 ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc
     2941 tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact
     3001 gctttcagag taacttctat atttccagct gtagcttttg aatacggaca cactttatgc
     3061 gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc
     3121 tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca
     3181 gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat
     3241 gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca
     3301 ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc agagcgtccg
     3361 cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc tttaattata
     3421 ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata
     3481 agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa
     3541 aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc tcaaaaagtt
     3601 gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat aaataaccaa
     3661 aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa
     3721 tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga
     3781 tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt
     3841 tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca ttattgcctc
     3901 tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta
     3961 gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc ataaagatct
     4021 cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta aagaaaacgc
     4081 attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc
     4141 gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta
     4201 tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg
     4261 actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac
     4321 ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc
     4381 gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag
     4441 cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg aatgcgtctg
     4501 taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga
     4561 tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa
     4621 tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta
     4681 ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag ggttttttac
     4741 ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata actatagctc
     4801 ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct ccagcagcaa
     4861 tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa
     4921 tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt ccttaattca
     4981 ttaataatac tcttatctta ttataactta cttttcacct taagttaact ttaattaata
     5041 aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac ttctacgcca
     5101 aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt
     5161 caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa gtctatctat
     5221 ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga
     5281 aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa aacacaagga
     5341 ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat
     5401 atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat
     5461 atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac
     5521 caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg
     5581 aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt
     5641 acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt
     5701 ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa
     5761 cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg
     5821 aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc gggggagatt
     5881 tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat ggcatctatt
     5941 tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg
     6001 tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa
     6061 gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt
     6121 ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt
     6181 ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat
     6241 atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc
     6301 aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc
     6361 gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta
     6421 cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct caagcttggc
     6481 tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg attaaagatt
     6541 attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa
     6601 tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg
     6661 ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta
     6721 tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg
     6781 aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc
     6841 tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg
     6901 aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat ttcacacgga
     6961 aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag
     7021 aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag
     7081 agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa
     7141 agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac
     7201 aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc
     7261 tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga
     7321 agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa
     7381 cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga
     7441 catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt
     7501 tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt
     7561 aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg
     7621 aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt
     7681 agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt
     7741 tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta
     7801 ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa
     7861 tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga
     7921 ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc
     7981 cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca
     8041 aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg
     8101 acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa
     8161 caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt
     8221 ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa
     8281 aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa
     8341 catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc
     8401 aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat
     8461 agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc
     8521 ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt
     8581 aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca
     8641 agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt
     8701 gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt
     8761 cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca
     8821 atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc
     8881 attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt
     8941 caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa
     9001 ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac gatcattttc
     9061 ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca tattccacca
     9121 gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca
     9181 tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca
     9241 actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa
     9301 ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact
     9361 atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt
     9421 cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg
     9481 aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat
     9541 gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc
     9601 gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg
     9661 gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc catgatctat
     9721 ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt
     9781 atttcctgga tctataaact tacgactgga acttctccgc agtattctat ggcttcagca
     9841 atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca
     9901 aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa
     9961 aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct
    10021 atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt
    10081 tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt
    10141 acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga
    10201 ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa
    10261 gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt
    10321 atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata
    10381 taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa
    10441 tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga
    10501 ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac
    10561 cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg
    10621 ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg
    10681 ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact
    10741 ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat
    10801 ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac
    10861 tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc
    10921 ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg
    10981 ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa
    11041 ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt
    11101 ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat
    11161 gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat
    11221 ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat
    11281 tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc
    11341 tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa
    11401 aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta
    11461 tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga
    11521 tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa
    11581 gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga
    11641 aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg
    11701 attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt
    11761 tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa
    11821 attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa
    11881 tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcttct ttggcactag
    11941 gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg
    12001 atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa
    12061 agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag
    12121 gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca
    12181 atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca
    12241 aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga
    12301 tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac
    12361 aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat
    12421 tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta
    12481 tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat
    12541 tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa
    12601 atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag
    12661 ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact
    12721 accaattgac attagaacat tggacaaaga attttcatca agtccaagat caagtcacga
    12781 aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt
    12841 catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg
    12901 acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag
    12961 aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc
    13021 taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta
    13081 aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag
    13141 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
    13201 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
    13261 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
    13321 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
    13381 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
    13441 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
    13501 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
    13561 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
    13621 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
    13681 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
    13741 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
    13801 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
    13861 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
    13921 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac
    13981 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
    14041 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
    14101 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
    14161 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
    14221 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
    14281 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
    14341 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
    14401 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
    14461 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
    14521 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
    14581 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
    14641 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
    14701 gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
    14761 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
    14821 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
    14881 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
    14941 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
    15001 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
    15061 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
    15121 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
    15181 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
    15241 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
    15301 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
    15361 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
    15421 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
    15481 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
    15541 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
    15601 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
    15661 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
    15721 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
    15781 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
    15841 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
    15901 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
    15961 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
    16021 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
    16081 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
    16141 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
    16201 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
    16261 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
    16321 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
    16381 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
    16441 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
    16501 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
    16561 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
    16621 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
    16681 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
    16741 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
    16801 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
    16861 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
    16921 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
    16981 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
    17041 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
    17101 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
    17161 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
    17221 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
    17281 ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
    17341 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg ttgcttttga
    17401 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
    17461 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg ttaccttatc
    17521 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
    17581 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
    17641 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
    17701 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
    17761 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
    17821 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
    17881 aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga aaaatagaaa
    17941 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
    18001 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
    18061 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
    18121 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
    18181 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
    18241 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
    18301 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
    18361 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
    18421 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
    18481 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaac
    18541 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
    18601 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
    18661 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
    18721 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
    18781 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
    18841 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
    18901 aaaaagcacc atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
    18961 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
    19021 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
    19081 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
    19141 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
    19201 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
    19261 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
    19321 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
    19381 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
    19441 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
    19501 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
    19561 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
    19621 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
    19681 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
    19741 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
    19801 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
    19861 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
    19921 agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc ccgtttacac
    19981 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
    20041 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
    20101 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
    20161 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
    20221 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
    20281 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
    20341 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
    20401 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
    20461 cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt cgatacattt
    20521 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
    20581 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
    20641 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
    20701 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
    20761 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
    20821 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
    20881 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
    20941 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
    21001 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
    21061 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
    21121 caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc agtcacaata
    21181 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
    21241 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
    21301 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
    21361 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
    21421 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
    21481 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
    21541 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
    21601 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
    21661 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
    21721 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
    21781 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
    21841 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
    21901 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
    21961 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
    22021 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
    22081 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
    22141 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
    22201 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
    22261 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
    22321 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
    22381 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
    22441 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
    22501 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
    22561 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
    22621 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
    22681 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
    22741 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
    22801 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
    22861 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
    22921 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
    22981 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
    23041 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
    23101 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    23161 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    23221 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    23281 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    23341 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    23401 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    23461 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    23521 caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    23581 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    23641 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    23701 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    23761 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    23821 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    23881 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    23941 aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta aatataattt
    24001 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    24061 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    24121 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    24181 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    24241 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    24301 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    24361 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    24421 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    24481 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    24541 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    24601 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    24661 tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca agaatcgtct
    24721 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    24781 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    24841 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    24901 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    24961 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    25021 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    25081 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    25141 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    25201 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    25261 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    25321 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    25381 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    25441 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    25501 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    25561 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    25621 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    25681 aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    25741 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    25801 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    25861 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    25921 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    25981 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    26041 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    26101 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    26161 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    26221 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    26281 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    26341 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    26401 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    26461 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    26521 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    26581 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    26641 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    26701 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    26761 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    26821 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    26881 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    26941 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    27001 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    27061 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    27121 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    27181 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    27241 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    27301 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    27361 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    27421 tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    27481 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    27541 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    27601 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    27661 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    27721 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    27781 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    27841 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    27901 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    27961 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    28021 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    28081 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    28141 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    28201 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    28261 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    28321 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    28381 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    28441 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    28501 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    28561 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aa
//
LOCUS       DAGCXG010000025        27675 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGCXG010000025 DAGCXG010000000
VERSION     DAGCXG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.026"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(239..1183)
                     /locus_tag="MB022_001150"
     CDS             complement(239..1183)
                     /locus_tag="MB022_001150"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4466160.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1479..2423)
                     /locus_tag="MB022_001151"
     CDS             complement(1479..2423)
                     /locus_tag="MB022_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466161.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2644..3858
                     /locus_tag="MB022_001152"
     CDS             2644..3858
                     /locus_tag="MB022_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4466162.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3880..5895
                     /locus_tag="MB022_001153"
     CDS             3880..5895
                     /locus_tag="MB022_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBT4466163.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5978..7351)
                     /locus_tag="MB022_001154"
     CDS             complement(5978..7351)
                     /locus_tag="MB022_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4466164.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7377..7661)
                     /locus_tag="MB022_001155"
     CDS             complement(7377..7661)
                     /locus_tag="MB022_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4466165.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7663..9678)
                     /locus_tag="MB022_001156"
     CDS             complement(7663..9678)
                     /locus_tag="MB022_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4466166.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9857..10162)
                     /locus_tag="MB022_001157"
     CDS             complement(9857..10162)
                     /locus_tag="MB022_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBT4466167.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10251..11282)
                     /locus_tag="MB022_001158"
     CDS             complement(10251..11282)
                     /locus_tag="MB022_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4466168.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11489..12364
                     /locus_tag="MB022_001159"
     CDS             11489..12364
                     /locus_tag="MB022_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4466169.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12516..13358)
                     /locus_tag="MB022_001160"
     CDS             complement(12516..13358)
                     /locus_tag="MB022_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4466170.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13450..15327)
                     /locus_tag="MB022_001161"
     CDS             complement(13450..15327)
                     /locus_tag="MB022_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBT4466171.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15324..16745)
                     /locus_tag="MB022_001162"
     CDS             complement(15324..16745)
                     /locus_tag="MB022_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBT4466172.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEELRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16748..20137)
                     /locus_tag="MB022_001163"
     CDS             complement(16748..20137)
                     /locus_tag="MB022_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBT4466173.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20394..21785
                     /locus_tag="MB022_001164"
     CDS             20394..21785
                     /locus_tag="MB022_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466174.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21858..22505)
                     /locus_tag="MB022_001165"
     CDS             complement(21858..22505)
                     /locus_tag="MB022_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4466175.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22513..23199)
                     /locus_tag="MB022_001166"
     CDS             complement(22513..23199)
                     /locus_tag="MB022_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466176.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23205..24278)
                     /locus_tag="MB022_001167"
     CDS             complement(23205..24278)
                     /locus_tag="MB022_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466177.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24500..25174
                     /locus_tag="MB022_001168"
     CDS             24500..25174
                     /locus_tag="MB022_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4466178.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25179..26519
                     /locus_tag="MB022_001169"
     CDS             25179..26519
                     /locus_tag="MB022_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4466179.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26668..27654)
                     /locus_tag="MB022_001170"
     CDS             complement(26668..27654)
                     /locus_tag="MB022_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBT4466180.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8061 a         5242 c         5027 g         9345 t
ORIGIN      
        1 tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc
       61 gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac attttcggac
      121 cgattaattt taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct
      181 taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt
      241 ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt
      301 cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg
      361 gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca
      421 atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac
      481 catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag
      541 caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg
      601 gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta
      661 agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga
      721 aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt
      781 cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt
      841 cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa
      901 ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat
      961 cagcatcttt acattcttca tagccacctg accatacagc cacattttca tcgccccaag
     1021 ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca
     1081 atactaattc attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa
     1141 aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa cagctccttt
     1201 tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg
     1261 ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac
     1321 agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact
     1381 gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa
     1441 caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca
     1501 ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat
     1561 ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca
     1621 aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat
     1681 ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa
     1741 gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca
     1801 tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat
     1861 gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata
     1921 ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta
     1981 tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt
     2041 gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa
     2101 atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg
     2161 tcactttctt ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac
     2221 atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt
     2281 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
     2341 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
     2401 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
     2461 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
     2521 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
     2581 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
     2641 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
     2701 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
     2761 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
     2821 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
     2881 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
     2941 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
     3001 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
     3061 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
     3121 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
     3181 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
     3241 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
     3301 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
     3361 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
     3421 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
     3481 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
     3541 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
     3601 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
     3661 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
     3721 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
     3781 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
     3841 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
     3901 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
     3961 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
     4021 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
     4081 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
     4141 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
     4201 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
     4261 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
     4321 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
     4381 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
     4441 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
     4501 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
     4561 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
     4621 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
     4681 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
     4741 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
     4801 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
     4861 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
     4921 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
     4981 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
     5041 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
     5101 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
     5161 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
     5221 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
     5281 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
     5341 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
     5401 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
     5461 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
     5521 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
     5581 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
     5641 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
     5701 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
     5761 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
     5821 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
     5881 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
     5941 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
     6001 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
     6061 tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg
     6121 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
     6181 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
     6241 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
     6301 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
     6361 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
     6421 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
     6481 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
     6541 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
     6601 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
     6661 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
     6721 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
     6781 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
     6841 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
     6901 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
     6961 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
     7021 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
     7081 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
     7141 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
     7201 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
     7261 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
     7321 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
     7381 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
     7441 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
     7501 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
     7561 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
     7621 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
     7681 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
     7741 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
     7801 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
     7861 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
     7921 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
     7981 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
     8041 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
     8101 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
     8161 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
     8221 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
     8281 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
     8341 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
     8401 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
     8461 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
     8521 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
     8581 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
     8641 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
     8701 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
     8761 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
     8821 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
     8881 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
     8941 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
     9001 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
     9061 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
     9121 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
     9181 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
     9241 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
     9301 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
     9361 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
     9421 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
     9481 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
     9541 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
     9601 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
     9661 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
     9721 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
     9781 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
     9841 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
     9901 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
     9961 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    10021 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    10081 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    10141 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    10201 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    10261 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    10321 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    10381 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    10441 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    10501 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    10561 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    10621 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    10681 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    10741 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    10801 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    10861 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    10921 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    10981 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    11041 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    11101 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    11161 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    11221 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    11281 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    11341 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    11401 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    11461 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    11521 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    11581 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    11641 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    11701 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    11761 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    11821 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    11881 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    11941 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    12001 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    12061 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    12121 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    12181 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    12241 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    12301 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    12361 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    12421 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    12481 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    12541 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    12601 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    12661 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    12721 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    12781 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    12841 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    12901 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    12961 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    13021 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    13081 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    13141 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    13201 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    13261 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    13321 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    13381 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    13441 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    13501 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    13561 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    13621 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    13681 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    13741 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    13801 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    13861 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    13921 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    13981 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    14041 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    14101 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    14161 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    14221 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    14281 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    14341 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    14401 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    14461 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    14521 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    14581 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    14641 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    14701 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    14761 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    14821 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    14881 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    14941 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    15001 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    15061 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    15121 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    15181 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    15241 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    15301 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    15361 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    15421 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    15481 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    15541 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    15601 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    15661 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    15721 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    15781 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    15841 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    15901 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    15961 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    16021 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    16081 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    16141 gtaaaatcat ttctaagctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    16201 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    16261 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    16321 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    16381 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    16441 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    16501 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    16561 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    16621 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    16681 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    16741 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    16801 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    16861 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    16921 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    16981 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    17041 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    17101 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    17161 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    17221 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    17281 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    17341 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    17401 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    17461 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    17521 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    17581 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    17641 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    17701 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    17761 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    17821 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    17881 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    17941 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    18001 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    18061 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    18121 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    18181 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    18241 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    18301 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    18361 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    18421 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    18481 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    18541 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    18601 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    18661 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    18721 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    18781 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    18841 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    18901 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    18961 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    19021 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    19081 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    19141 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    19201 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    19261 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    19321 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    19381 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    19441 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    19501 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    19561 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    19621 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    19681 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    19741 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    19801 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    19861 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    19921 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    19981 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    20041 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    20101 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    20161 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    20221 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    20281 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    20341 gggttgaggg agaaaaacat atgatcaggt tgttggaatg aaactataga aagatggaaa
    20401 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    20461 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    20521 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    20581 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    20641 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    20701 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    20761 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    20821 tgcgtatctt tgtcaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    20881 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    20941 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    21001 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    21061 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    21121 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    21181 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    21241 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    21301 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    21361 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    21421 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    21481 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    21541 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    21601 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    21661 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    21721 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    21781 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    21841 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    21901 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    21961 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    22021 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    22081 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    22141 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    22201 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    22261 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    22321 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    22381 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    22441 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    22501 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    22561 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    22621 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    22681 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    22741 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    22801 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    22861 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    22921 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    22981 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    23041 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    23101 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    23161 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    23221 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    23281 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    23341 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    23401 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    23461 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    23521 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    23581 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    23641 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    23701 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    23761 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    23821 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    23881 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    23941 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    24001 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    24061 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    24121 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    24181 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    24241 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    24301 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    24361 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    24421 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    24481 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    24541 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    24601 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    24661 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    24721 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    24781 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    24841 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    24901 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    24961 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    25021 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    25081 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    25141 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    25201 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    25261 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    25321 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    25381 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    25441 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    25501 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    25561 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    25621 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    25681 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    25741 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    25801 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    25861 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    25921 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    25981 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    26041 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    26101 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    26161 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    26221 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    26281 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    26341 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    26401 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    26461 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    26521 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    26581 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    26641 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    26701 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    26761 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    26821 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    26881 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    26941 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    27001 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    27061 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    27121 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    27181 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    27241 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    27301 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    27361 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    27421 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    27481 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    27541 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    27601 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    27661 tcctcctagc aaata
//
LOCUS       DAGCXG010000026        26787 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGCXG010000026 DAGCXG010000000
VERSION     DAGCXG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.027"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            205..744
                     /gene="pyrR"
                     /locus_tag="MB022_001171"
     CDS             205..744
                     /gene="pyrR"
                     /locus_tag="MB022_001171"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBT4466181.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            758..2041
                     /locus_tag="MB022_001172"
     CDS             758..2041
                     /locus_tag="MB022_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBT4466182.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            2054..2980
                     /locus_tag="MB022_001173"
     CDS             2054..2980
                     /locus_tag="MB022_001173"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBT4466183.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            2999..4279
                     /locus_tag="MB022_001174"
     CDS             2999..4279
                     /locus_tag="MB022_001174"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBT4466184.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            4284..5360
                     /gene="carA"
                     /locus_tag="MB022_001175"
     CDS             4284..5360
                     /gene="carA"
                     /locus_tag="MB022_001175"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBT4466185.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            5365..8550
                     /gene="carB"
                     /locus_tag="MB022_001176"
     CDS             5365..8550
                     /gene="carB"
                     /locus_tag="MB022_001176"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBT4466186.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            8604..9380
                     /locus_tag="MB022_001177"
     CDS             8604..9380
                     /locus_tag="MB022_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBT4466187.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            9377..10309
                     /locus_tag="MB022_001178"
     CDS             9377..10309
                     /locus_tag="MB022_001178"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBT4466188.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            10306..11022
                     /gene="pyrF"
                     /locus_tag="MB022_001179"
     CDS             10306..11022
                     /gene="pyrF"
                     /locus_tag="MB022_001179"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBT4466189.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            11029..11658
                     /locus_tag="MB022_001180"
     CDS             11029..11658
                     /locus_tag="MB022_001180"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBT4466190.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            11646..11960
                     /locus_tag="MB022_001181"
     CDS             11646..11960
                     /locus_tag="MB022_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466191.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            11998..12639
                     /locus_tag="MB022_001182"
     CDS             11998..12639
                     /locus_tag="MB022_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4466192.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            12682..13374
                     /locus_tag="MB022_001183"
     CDS             12682..13374
                     /locus_tag="MB022_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBT4466193.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            13371..14273
                     /locus_tag="MB022_001184"
     CDS             13371..14273
                     /locus_tag="MB022_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4466194.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(14431..16137)
                     /gene="efbA"
                     /locus_tag="MB022_001185"
     CDS             complement(14431..16137)
                     /gene="efbA"
                     /locus_tag="MB022_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBT4466195.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            16327..16950
                     /locus_tag="MB022_001186"
     CDS             16327..16950
                     /locus_tag="MB022_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466196.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            17400..18449
                     /locus_tag="MB022_001187"
     CDS             17400..18449
                     /locus_tag="MB022_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4466197.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            18446..19327
                     /locus_tag="MB022_001188"
     CDS             18446..19327
                     /locus_tag="MB022_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466198.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            19330..20100
                     /locus_tag="MB022_001189"
     CDS             19330..20100
                     /locus_tag="MB022_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4466199.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            20379..21233
                     /gene="aroE"
                     /locus_tag="MB022_001190"
     CDS             20379..21233
                     /gene="aroE"
                     /locus_tag="MB022_001190"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBT4466200.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            21411..22574
                     /locus_tag="MB022_001191"
     CDS             21411..22574
                     /locus_tag="MB022_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4466201.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            22691..23101
                     /locus_tag="MB022_001192"
     CDS             22691..23101
                     /locus_tag="MB022_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4466202.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            23275..25503
                     /gene="topB"
                     /locus_tag="MB022_001193"
     CDS             23275..25503
                     /gene="topB"
                     /locus_tag="MB022_001193"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4466203.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            25674..25958
                     /locus_tag="MB022_001194"
     CDS             25674..25958
                     /locus_tag="MB022_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466204.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            26118..26354
                     /locus_tag="MB022_001195"
     CDS             26118..26354
                     /locus_tag="MB022_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4466205.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            26351..26749
                     /locus_tag="MB022_001196"
     CDS             26351..26749
                     /locus_tag="MB022_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBT4466206.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         8821 a         4672 c         5928 g         7366 t
ORIGIN      
        1 aagcataaaa cttaatagaa ttcctttaag tttagtcccg tgaggctaag aagggtggga
       61 gcaaggatac gtatggtgcg ccataagtag attgctcatt gacatagtat agctatcagt
      121 gtatcagtat acgctcagtc taaacattcc tcctgtcctc ttagggcagg agttttttta
      181 tgcaaaaaag tagggggaat gaaaatgcaa gcaaaagaag tagtagatca ggtaaccatg
      241 aagcgagcat tgactcgaat tacttatgag attatcgaga gaaaccatag tatccaagat
      301 atcgtattgg taggtatcaa aacacgagga atctacattg catcaagaat tgccgaacgc
      361 ctgaagcagc tagaagatat cgatattcct gtaggagaac tggatatcac tttataccga
      421 gatgacaaaa aagaaaatcc agaagaaccg gaacttcatt cttctgacat cccagtttcc
      481 ttagaaggaa aagaagttat tttgattgat gatgttttgt atacaggacg aacgattcga
      541 gcagcaatgg acgcagtaat ggattttggc agaccaagaa aaatttcatt agcagtcctg
      601 gtcgatcgtg ggcatcgaga attgccaatc cgtgcagatt atgttgggaa gaacattcca
      661 acatctcggg cggaagagat tttagtagag atgcaagagc ttgatggaca agatcgcatc
      721 atgattttga aagaggaaga ttagaggaga gagaaacatg tcggagaaaa aaggatttca
      781 taatgatgac gtggtgttag atatccacga tcgcccaacc ccaggacatt gggtaggatt
      841 aagtttacaa cacttgttca cgatgttcgg agcaaccgtg ttagtcccca tcttagtagg
      901 tatcgatcca ggtattgcgc tagtcagttc aggactaggt acattggtgt acttatttac
      961 tactaaagga aagatacctg cgtacttagg aagcagcttc gctttcattg cagcgatgca
     1021 gcttttgatg aaaacagatg gttatccagc gattgcacaa ggagcagtca caactggatt
     1081 agtctatttg attgttgcgc tgatcatcaa aaagatcggt tcagcatggc tggataaaat
     1141 cttgcctcca atcgtcgtcg gtccagtcat catggttatc gggttgggac ttgcggcaaa
     1201 tgcagcaaca aatgctatgt ataatcaggg cacgtatgat ttcaagtata ttgctgtagc
     1261 gctgatcaca ttaggattaa caattttctt caacatgtgg ctaagaggtt ttctcggatt
     1321 gattccgatt ttactaggta ttattagcgg atacatcgta gccttgctgt ttggaatcgt
     1381 ggatactaag ccaatcgtag acgctgcctg gtttgctgtt cctaactttg agattccctt
     1441 tgtacaatat gaaccaaagc tatatcttgg agcaatcaca acaatggcac ccatcgcttt
     1501 tgtgacgatg acagagcata tcggccatct tatggtattg aataaactaa ctaaacgtaa
     1561 tttctttgaa gaaccgggtc ttcataagac gttgacaggt gatggattag cacaaatcgt
     1621 ggcagccttt gtcggaggtc ctcctgtaac aagttacggt gaaaacattg gtgttctggc
     1681 tatcacacgt gtccatagcg tgtttgtgat cggtggagct gcagtatttg cagtcatttt
     1741 aggattcgta ggaaaattaa gcgctttgat tttaagtatc cctggaccag tcatctcagg
     1801 aatcagtttt atcttatttg gagtaattgc cgcaagtgga atgaagattt taatcgagaa
     1861 caaaatcgat tttgataaaa agaaaaatct gttgattgct tcagttatct tagtagtagg
     1921 aattggcgga ctcgtctttg aagtaggcac atttacattg tcggcaatgg cattagcaac
     1981 tgtacttggc atcattttaa atcttatctt accagaaact tcacgaagcg aagaaaacta
     2041 aggaggaaac attatgatca tcaaatcaga acgtatcagc ttaaagcatt tattgaccgt
     2101 tgaggcactt actgatcaag aagttatggg cttgatccgc agagggcagg aattcaagca
     2161 aggtgctaat tggactccgc aaaagcgcca gtactttgct acaaacctct ttttcgaaaa
     2221 tagtactcgc acacataaaa gcttcgatgt agcggagaaa aaactagggc tagaagtgat
     2281 tgaatttgaa gcaagcacaa gctctgtcca aaaaggggaa acactatacg atacagtact
     2341 gacgatgtca gcattaggcg tagacgtagc ggttatccgt catggtgatg agaactatta
     2401 tgacgaactg atacaaagta aaacaatcca gtgttcgatt atcaatggag gagacggaag
     2461 cggacagcat ccaacccaat gcttgcttga tttgatgacc atttatgaag aatttggtca
     2521 ttttgaaggt ctgaaagttg ccatcgtagg tgatatcact cactctcgcg tagctaaatc
     2581 caatatgcag atgttaaaac gtctaggcgc acaagtattc ttctcaggtc ctagagaatg
     2641 gtacgacgat gaattcgaag tgtacggaca ttatatgcca ttggatgaac tactagatca
     2701 agttgatgtc atgatgatgc ttcgggtgca acacgaacgc catgatggaa aagaaagttt
     2761 ctcaaaagaa ggctaccatc aggaacatgg gttaacagtt gagcgtgcga agaagttgca
     2821 aaaacatgca atcattatgc acccagcacc agttaaccgt gatgtagaat tagctgattc
     2881 attagtggaa ggattacagt cgcggatcgt tcagcaaatg agcaatggag tattcgtcag
     2941 aatggcaatc ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg atgaaaaaat
     3001 gaaaacactc attaaaaacg gtcagatcaa tacacgaaaa aatatgacga caccagcaga
     3061 aatttggata gaagatgggc gaatcaaagc aatcggtaca ggattctcag aagccgaatt
     3121 tgatgaagtg tttgatgcaa agggacaatt gatcacacct ggtttagtgg acgtccatgt
     3181 tcatttgaga gagccaggat ttacatacaa ggagacgatc gaagctggga ctcgctcagc
     3241 tgctcgtggc ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg tacctgatac
     3301 ggcagaaaag ctgagacaag tatatgacat cattagaaaa gatgcagttg tgaaggtatt
     3361 gcagtatgcg ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc aagaagcact
     3421 gattgaagaa ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa cagctgggac
     3481 gatgtatctg gctatgaaag aagctgcaaa aaacaataaa gccctagtag cccatacaga
     3541 agatgaatct ttattatttg gcggcgttat gcacgctggc aagaaggcag aagaacttgg
     3601 tcttcccggg atcttaagcg taaccgagtc ttctcaaatt gctagagatc tgttgctagc
     3661 tgaagcaact ggtgttcatt accatgtttg ccatgtttct actaaagaaa gtgtccgtgt
     3721 aatcagagac gcaaaaaaag caggaatcca cgtaacagca gaagtttcac cccatcatct
     3781 tattttgatc gacgaagata ttcccgaaga tttcggcttt tggaaaatga acccgccgct
     3841 aagaggacgg gaggacagag aagctttgat cgaaggcttg cttgacggta cgatcgattg
     3901 catcgctaca gaccatgccc cccatggatt agaagaaaaa agccaaagtt tcatgaaatc
     3961 accatttggt atcgtaggaa gcgaaacagc gtttcaactg atctatactc attttgtaga
     4021 aactggtcga ttcacgttgg aacaagtgat caattggttg gcagttaaac cggctgagat
     4081 ttttggtttg aatgcaggaa cgctaacggt tggtgccccc gctgatgtag cagtttttga
     4141 tattacacaa acttgcacga tcgataaaga agacttttta tcaaaagggg agaatacacc
     4201 ttttatcggc tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg gaaaacttgt
     4261 gtggcagaag ggagaataaa agtatggaac gtttgcttat attagaagat ggaacagttt
     4321 tcgaaggaaa ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt tttacaacaa
     4381 gtatgactgg ttatcaagaa actatcactg atcaaagttt taacggtcaa atcattacat
     4441 ttacctatcc tatggtgggt aattatgggg taaatagaga tgattatgaa tcgatttctc
     4501 caacatgcaa aggagtagta gtaaaagaac atgcacgtgt agcttcaaac tggcgaaacc
     4561 aaatgacgct agattcattt ttgaaacaaa aaggaattcc tggaatatca ggtatcgata
     4621 cgcgcgccct tactcgcaaa atccgtgaag tcggaacgat gaaagctagt atagttgatg
     4681 ctggagattc cttcgaacat gcatttgatc agctgaaagc aaccgttcta gcaaccaatc
     4741 aagtgcagca agtctcaact aacaaacctt atccaagtcc aggaacaggt cggaaagtca
     4801 ttgtcattga cttcggcttg aaacatagta ttttacgaga attgagtaaa agaaactgta
     4861 acctcacagt acttccctat aacacagatg ccgagacgat tttagcgcta tcaccagacg
     4921 gagtcatgct gacgaatgga cctggtgatc caaaagatgt gccagaagca atcgaaatga
     4981 tcaaaaaaat ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac cagttatttg
     5041 ctttagccaa cggtgcagat acctataaaa tgaaattcgg acatcgagga ttaaatcacc
     5101 cggtacgaga agtagcgacc ggaagaattg attttacttc tcaaaatcat ggctatgcag
     5161 tcgatgaagc cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa gtaaatgacg
     5221 gaacagtaga aggagtcaaa cacagagatt atcccgcttt cagtgtgcag tttcatccag
     5281 atgctgcccc tggtccgcat gatgcactac atttatttga tgaatttatg gaaatgatgg
     5341 acgcaggaaa ggagcagtaa acagatgccg aaacgtacag atatcaagaa aattatggtg
     5401 attggatctg gtccgatcat cattggtcaa gccgcagaat ttgactacgc aggaacacaa
     5461 gcctgccttg ccttaaaaga agaaggatat gaagtggttt tagtaaattc taatcctgcg
     5521 acgatcatga cggataaaga agtggcagat caagtatata tcgaaccgat cacacttgaa
     5581 tttgtatcaa ggattcttcg aaaagagcgt ccagatgctc tccttccaac tttaggcgga
     5641 cagacaggcc tcaatatggc aatggagctg gcagcatcag gtattttaga agaattgaac
     5701 atagaacttc taggaacaaa gctttctgcg atcgatcaag cagaagatcg tgatctattc
     5761 aaacagttga tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt caatacagta
     5821 gaagaagctg tagattttgc aaataaaatc ggctatcctg tcatcgttcg tccagccttt
     5881 actttaggcg gaactggtgg aggtatgtgt gaaaatgaag aagaattacg tatcgtatca
     5941 gaaaatggat taagtctttc ccccgctacc caatgtttga ttgaacgaag tattgcaggt
     6001 tttaaagaaa ttgaatatga agtgatgaga gattcggctg ataatgcaat cgttgtctgc
     6061 aatatggaaa attttgaccc ggtgggtatc catacaggtg attctatcgt atttgcccct
     6121 agtcaaacat tgtctgacca tgaatatcaa atgcttcgtg atgcttctct ttcgattatt
     6181 cgagcactta agatcgaggg aggctgtaat gtccaactcg ctcttgatcc acatagtttt
     6241 aattattatg tgattgaagt caatccgcgt gtttctcgtt catctgcgtt agcaagtaag
     6301 gcgactggct atccgatcgc taagctagct gctaaaatag cgcttggatt aacattagac
     6361 gaaatgaaaa atcctgttac aggaacgact tttgcagaat ttgaacctgc attagattat
     6421 gttgtagcta aaattcctcg ctggccgttt gataagtttg aaaacggaga acggctttta
     6481 ggcacgcaga tgaaagcaac tggtgaagtg atggctatcg gtcggaatat cgaagagtct
     6541 ttattgaaag ctgtgcgttc tttagaaatc ggtacacacc atatcgaact tcctgagttg
     6601 gcagacatca cggacgatga cctaatccaa aaagtggttc gagcacaaga tgatcgcctg
     6661 ttttatgtag ctgaagctat tagaagaggg tacaccatag aagaattagc ggatttgaca
     6721 aaaatcgatt tattcttttt agataaactg ttgcatatcg tagaaatcga acagcagttg
     6781 aaacagcagc cgttggatga caagatattg aaaacagcga aacaaaacgg ttttacagac
     6841 aaaaaagtgg cggagctgtg ggaaataaac gaagaagccg ttcgccagat gagaaaagaa
     6901 aatgatttga ttcctgtata taagatggtt gacacttgtg cagcggaatt cgaatcaaaa
     6961 acaccttatt tttatagttc ctatgaatat gaaaatgaaa gttcacgatc agaaaagccc
     7021 tcaattctag tactgggatc tggtcctatc cgtatcggac aaggggttga atttgactat
     7081 gcgactgtgc attctgtcaa agcaatccag caggcaggat acgaagcaat catcatgaac
     7141 agcaatcctg aaacagtttc gactgacttt tctatttcgg ataaattata ttttgagcca
     7201 ttgaccttcg aagatgtgat gaatgtgatc gagctagaac agccgatagg agtaatcgtt
     7261 caatttggtg gtcagacagc aatcaactta gccgaacctt tgactgctgc tggtgtaaaa
     7321 atcttaggga caacgatcga agacttggat cgtgcggaaa atcgtgattt attcgagcaa
     7381 gcgctgaaag agttggatat tcctcaacct ttaggggata ctgctacaag taaagaagaa
     7441 gccgtggtta tcgcttcgag aattggttat ccagttcttg ttcgtccaag ctatgtatta
     7501 ggcggacgtg cgatggaaat cgttgaaaac cagcgagatt tagaagatta catggaacat
     7561 gctgtgaaag catctccaga acatccagta ctagtggacc gttacttgct tggtggtgaa
     7621 tgcgaagtgg atgcgatttg tgacggtgaa acagtactca tcccagggat catggagcat
     7681 attgagcgtg ctggtgtcca ttcaggggat tcaatggctg tgtatccacc tcaaacatta
     7741 tctgaatcga tcaagaaaac gatcgaagat tacacgatcc gcttagcatt aggtttgaat
     7801 tgtgtcggga tgatgaatat ccagtttgtc atccatgaaa atgaggttta tgtgatcgaa
     7861 gtcaatcctc gagcaagtcg gactgttcca ttcttaagta aggtgacagg aattccaatg
     7921 gcacaaattg cgacgaaagc tattttggga gagaaactaa agacacttgg ttatgaaaac
     7981 ggactttatc cagaaacaaa agaagttcat gttaaagcac ctgtcttttc ttttacgaaa
     8041 ttgcagaaag ttgatactta cttagggcct gaaatgaaat cgactggaga agtaatggga
     8101 tctgacagac aattagacaa agctttgtac aaggcattcg aagcttctgg attacacttg
     8161 cctgaatatg gagccgtatt atttacgatt gcggacgaaa caaaagaaga agcactagaa
     8221 ttagctaaac gttttacaga aattggctat agtctgatag gaacgaaaca tacagctgct
     8281 tatttcgaaa aaaatggtct ggtcgttgaa acagtagcga aaatttctga ggaagcagca
     8341 gaaaaaaatg tagtcgactt aataagagat ggtaaaacac aagtggttgt caatacgatt
     8401 gataaagatc ggggaaatgc ttctaaagac ggtttcatca tccgaagaga agcagttgaa
     8461 catggtattc cattgtttac ttctttagac acagcagatg cgatcgttcg agtgatggaa
     8521 tcccgtgctt tttcaactaa ccctatttaa cggcgagcac atgttaagac aaaaagacga
     8581 aaaaccgttg caggagggga cagatgaaac aagaaatgat ggaaatcgtt cagcaaaaac
     8641 agctggcacc aaagattttt gaaatgacat tgaaaggtga tctagtaaaa gaaatgcagc
     8701 aaccaggaca gttcttgcat attctcgtgc cgcgtgcaga cttgctatta cgccgaccga
     8761 ttagcttgaa tcatatcgac cacgaaacaa atacttgccg tatcatttat cgggtggaag
     8821 gtgaggggac tagagtcttc tctactcttt ctgccggaga ccatttagat gtcatgggtc
     8881 ctttaggcaa tggttttgat ttgtccaatc ttcatacagg agacgaagta tttataatcg
     8941 gtggaggaat cggtgtccct cctctatatg agttatcaaa acagttagtc gaaaaaggaa
     9001 ttaagccaac gcatttttta ggttttgcca cacatgaagt cgtttattat gaaaaagaat
     9061 ttaatcgtct aggagaaaca agaatcgcta ctgacgatgg aacatacggt atgcacggaa
     9121 atgtcggtaa tttattgtta ggaacgaaga cggaacctac agctgttttt gcatgtggtt
     9181 caaacgggtt attgaagaca gtggaacagc tgttttcttc gcatcaaaat gtccagttat
     9241 cattagaatc ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt tgtcatacgc
     9301 cggaagatga aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg atttttaaaa
     9361 taggagaggt aattatatga atgagaatcc tttagcggtc aaacttcctg gcctagaatt
     9421 aaaaaatcct atcatgccgg caagcggctg tttcggtttt ggcaaagaat acggaaaata
     9481 ctatgatttg aatcagcttg gatcgatcat ggtcaaggca acgactccaa atgcccgctt
     9541 tggcaatccc acgccaagag tggcggaaac accatctggc atgttgaatg caattggttt
     9601 gcaaaatcct ggtctagacg ttgttatgca taaacttctt ccagaattag aggagtacaa
     9661 tgagttaccc atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg tagaagtttg
     9721 tagcaaaatt ggcgaagcac caaatgtgaa agcaatcgag ctgaacattt cttgcccaaa
     9781 tgtcaaacat ggcggaattg cttttggaac agattctgat gttgcttttc aattaacaca
     9841 agcagtaaag aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa atgtaacaga
     9901 catcgttcca atcgctcagg caatcgaagc tggaggggca gatgggttta cgatgatcaa
     9961 tactcttctt ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag ccaatcaaac
    10021 aggtggactc tccggaccag caatcaagcc tgtcgctatt cgtttgattc accaagtagc
    10081 aagtatatcc agccttccaa tcattgggat gggcggcgtg cagacagtgg atgatgttct
    10141 tgagatgttt atggctggag caagtgctgt agctataggg accgcgaatt tcaccgatcc
    10201 gtatatctgt cctaaactaa ttaaagagct ccctgtgcga atgagcgaac taggcatcga
    10261 atcgctggaa agattaatca aagaagtgag agaggaacgt gaacgatgaa tcaactagac
    10321 caacgaccaa ttattgcatt agatttttca actaggcaag aggtagaaga ctttcttcga
    10381 tttttcccga aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt ctaccaagaa
    10441 ggacctgaaa tcgtacgtta tctaaaagag gctggtcata atgtattttt agatctgaaa
    10501 cttcacgata ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa gcttggtgtt
    10561 gatctcacct gtgttcatgc cgctggcggt attcgtatga tggaggcggc aatgcgagga
    10621 ttagaagagg ggactccaga aggtggaaaa cgtccgctac ttctagctat cactcagctt
    10681 acttctacaa gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc tttagaaaaa
    10741 agtgtgatcc attatgctag ctgtgccaaa aaagcaggat tagatggtgt ggtttcttct
    10801 gcttgggaag tagaagcgat caaagaaatg gcaggagacg aatttgtttg cttaactccg
    10861 ggtataagac cagaaggaac agtcgcagga gatcagacaa gagtagtcac accttctcag
    10921 gcaaaaaaga tcggctcaac ttttatcgtt ataggtcgac caatcactca gtcaactaat
    10981 ccttataaag catatcaaac gattcaaaca gaatggagct aacctaaaat gaatgttgaa
    11041 caaagtatcg caaaagactt attagaaatt gaagcagtat ttttaaatcc aagtaatcct
    11101 tttacatggg caagtggaat caaaagccca atttactgtg ataaccgaat cacgatgagc
    11161 tatccaaaag tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa agaagcttac
    11221 cctgaagtac aagtgattgc tggaactgca acagcaggaa tccctcatgc cgcatgggta
    11281 gcggaaatac tggatttgcc aatggtctat attcgaagta aggcgaaaga tcatgggaaa
    11341 ggtaaccaaa tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat cgaggatttg
    11401 atttctacag gcggaagtgt cttggaagca gctgaagcag caaaaagaga aggtgcggat
    11461 atccttggtg ttgcggctat ctttacgtat gagttgccaa aaggaaaagc aaatttcgaa
    11521 aaagccgaaa tccctcttat gacgttgacc aattattctg tattgattga agcagcttta
    11581 gaagatcgtt atatcgatga gcaagaatta actctgctaa aagaatggaa aaaagatcct
    11641 gaaaattggc agatttaagc gcgcttgatg aagatagaga aatccgattt gtgtctccag
    11701 aatcatcaat tggaatagca aaaatccaaa aactgattgc gagaagcatc ttttcatcag
    11761 gtatgaaaag aaacttctat cgtgatcagt ttttgatttt tttaaaattc atttttctaa
    11821 gactggttag cagaaagaag agaataatct cttctctttt ttctattata tatagatttt
    11881 ctctattttt tggtaaggga gctgtttcaa aaatggtaaa gagcggaaat gttgcgaata
    11941 tcagttattt aatgatatga tattcggaaa aagaaatctc aaagggaggt aacttttatg
    12001 gggttgaatg aatttatttt aggattgaac aatttagaga cgatcacaag ggcacctgga
    12061 ttttttaaat atacagagca tacagttgca gcacattcgt atcgtgtagc atctattgcg
    12121 caagttctag gagacattga agaagcgaat ggacaaccga tcgattggaa actgctctat
    12181 gaaaaatcat tgaatcatga ctacacagag cgttttattg gtgatatcaa aacgccggtg
    12241 aaatacgcaa gcaaagagtt aagagggatg ctgcaaacag ttgaagaaaa gatgacggac
    12301 gaattcatca accaagagat tccagctgag ttccaagaag tttatcgccg tcggttgttc
    12361 gaaggaaaag atgaaaccgt agaaggagaa ctattagcca tttcagataa ggttgacctt
    12421 ctttatgaat cttttgatga aatcatcaaa aataacccgg aaaaagttta taaggagatg
    12481 ttcattgagg cagtgactac gattaaaagc tttgaacatc ggccttctgt tcagtatttc
    12541 tttaaagaga tatttccgga actattgaac caggaatttt atggaaaaga ggcgttttta
    12601 gaaaccgtct caacgattgt aaagaaagaa gaaaaataaa aatgatatga gatgcagggg
    12661 gaagtatagt tgaaaaagaa tatggatgtc gaatggggct attctggaga caaaggtccg
    12721 gaaaattggg cgagtttatg tgattggttt gcgagaggtg cggaatttcc attgcagtcg
    12781 cctattcatt tgaccaaggg ggaagaaacc aaaatagaag gagacacgct ttcctttcat
    12841 tatcaaaagc aacggtttac tgacaaggaa ttcaaaaaca cgattcattt agtacctttt
    12901 gaccaactca gttatgtaga gttcaagaaa gaacgctatt atttgacaga tattcatttc
    12961 cacttgcctt ctgaacatat catcaacgga aaacaggaag atattgaatt tcattttgtt
    13021 catatgaaca gcaaaaaaga gaatctagtt gtgggtgtca tgtaccagct aatggtacag
    13081 gaaggttggc tgtataatca agaaaatggg gaaagttgga acttagaaaa acatgagcat
    13141 tgggtaaatc cggatgtgtt ctttccggag caaaaaagcc atttccatta tattggctca
    13201 ttgacaactc cgcctaccag cgggccaatc caatggtttg taatggatca tgtaggaaaa
    13261 ctgaaccaag agtttcttct ccccttcgat ggtcagtatg ctcgaccgaa caaccgacca
    13321 atccagccct tgaatggaag agaaatttat tatttcgagg agtttgagca atgaatattc
    13381 agcagatgaa atacgtggtc gctgtagcaa acaatggtag tttcagagaa gcggcaaaga
    13441 aaatgtttgt ctcccagcca agtctttcag ctggtatcaa agaattagaa accgagttag
    13501 ggatcacgct gtttactcga acgaatcgag gggcatattt aacagaagaa gggcaagact
    13561 tcttaaatcg tgccgagaaa atattggtcc agcttgatac attagaaact cactatctaa
    13621 cgagtgaaaa gagagacaat ttttcgatcg ctgcccagca ttatgatttc ttaggtcctt
    13681 tgaccgcaca gctactcgag gaattcaaag acgagatcaa acaatttcgg atcatggaga
    13741 cgacaacagc gagagtgatc gaagaagtca aagagcagca cagtgagatc ggcattattt
    13801 atatgaatga gcataatcaa gctggtatca gaagatattt acaacaagga gagctaagtg
    13861 gccgcagcat tggaaaattc aaaacgcata tttttttaag gcaaggacat ccattagctg
    13921 aacacgcaga gatcaccaaa gaagaattgt tatcttatcc tcaagttcgt tttacacagg
    13981 atggaaataa tttcccgtat ttttatgaag accttatcga gatccctgat caagaaacag
    14041 tgatttatac gagtgatcga ggtactttga tgaatatcgt tcttgaaact gatgcttacg
    14101 cttcaggatc tggtattgtg attggaggga ttcgcgagca cttgcggtta atccctttgg
    14161 cagaaggcga actaaatgaa ttttatatca tttattcggc gaaacgtccg ctcagtgaaa
    14221 tcgctcaaag atttatcaaa gagcttactc ggttactgga ccagcagagc taagaaaagc
    14281 agatgagaac gctgtttatt gaacaccgtt gatcaaaaaa gaggggttgt gacagaattt
    14341 tgtcacaact cctctttttg tagcaaacaa tttataacta attgctgttg tgggaaagta
    14401 cgcaaatatg attctagaaa cactcattga ttacctgttt tgtttcatag ctgtaatttt
    14461 ttcttcttct ggtgtcacga taatcgtctt ttggttttca taaataacat aaccaggctt
    14521 ggcgccattt ggttttcgga tatgctttac ctgtacaaga tcaaccggca cttgggcaga
    14581 gtatcggtat tttgaaaaat aggcagctaa ctcggcagct tctgtaattg tttcatctga
    14641 gggcttgtca cttttaatga tgacgtgcga accgggtata ttttttgcat gaagccaata
    14701 gtcggttttt ttagctgtct tcattgtcag ctggtcattt tgaagattgt ttttaccaac
    14761 aagaatgagc gttccgtcag aagaaaagta ttgatcaggc tgagatggtt tcttacgttt
    14821 ttgttttttt aagttcttct ttttcaggta tccttctgaa gtcaattctt ctttaattgc
    14881 ttcgatatcc attgggccgg caatttccag ttgagataag acagattcaa gatattggat
    14941 ttcatctttg gcttcttgga tttgttcgcc aatcagctta acagcgttct tcagcttttg
    15001 gtatcgatgg aaatatttct gtgcattttg atttggtgtc aatgccggat ctagtgctat
    15061 tttgattggc cgatcttctt cataataatt aggtaaaaca acttcttttg cgccgcgtgg
    15121 tacttgtgtc agaaaagtag taagtagttc gccatttcta cggtaattct cagcgttttc
    15181 ggattctttg agtgtttgtt cacgtttttt caatttattt ttatttcgtt ttagttcatt
    15241 ttcgattttt cgaatcagtt ctccgccttg ttgttttgcc cgatctttct ctgctttttc
    15301 gtgataatag ctatctaata attgactgag tgttggataa gctgttacag cagatgcctg
    15361 ttcggacaat acctgataag caatcggtgt gaaaaaatct ttattttccg tttcgtaaaa
    15421 agtaggaata ggctgatggc tgatggctga gaaaaattcg tcccatacaa ccatcttttc
    15481 attaggacgt tccagtaatc ttttggtcag ctcttgagcg atatcgaaac cgattccctg
    15541 aaactgacgt tggattgctt ttggatctag ttcagtttgg gaaagccgct gaaagatttt
    15601 ttctttctcg gagctaaatg ggttcaagac ctcttgtttt ggcggagcaa catattctac
    15661 accaggaagc aaagaacgat aagtgttttg agagcttcca atgtgtttga tcgcatccag
    15721 tatttttcca gtttctcgat taactaagac gatcgtacta tgacgtccca ttaattcaac
    15781 gatcaatatg atattttgta gatcacccaa ctcgtttcgt ttagcaaagt ggaaatggat
    15841 cactcgatca ttttcgattt gttcgatgtc ttctaaaata gcgccatcca aatattttcg
    15901 aagcatcatc acgaagttag gcggattatc aggattctga taatcgattt gggtaatctg
    15961 tacacgagca taacttgggt gcgcagataa taataatcga tggtttttgc cgcgtgaacg
    16021 aatcaccagc acgatttcat tttcataagg ctgatggatt ttcgagatcc ggccagatag
    16081 taacgtttca cgcaattcgt tgaccatcgc gtgggtaaat acaccatcaa aggacatggt
    16141 tcattcctca tttcttttca taagtatatt cattataacg aaaaaaagaa gaaaaaccaa
    16201 tttggccgaa tagtaaagca aaagtctaga aaaatcttgg actaattaca taaaaaacag
    16261 tgtttctatc gttgaaatcc aaatttttag gtataataaa aaataggaga aaaagtagaa
    16321 aagaaggtgt ttgttatttg ggaagtagtt aaatcgagag tacaagaaat gaatgaacat
    16381 ttctcagtgt cattcgcaga aaatccaagt aagtattttc gacgtcttgt ttttctgctc
    16441 atcttatcat tgctgttaac agttgcaatg tttttattgc cggaaaaaat ccgagaagag
    16501 caagtaaata gtctcgttta ttcgtccata ccaaccagca aattgaaacc ttttgattac
    16561 agcaaagctg atcagaaaat caaacaaagc cgggcgatat cggtgatgtt cagcaaaccg
    16621 aacgggaaaa cctatcagga catgcttgat gtttttaatg atcctgagct tatggaagaa
    16681 ttgaatcgac cgatctttta ttatccgatc gtttatgatg tacatgaact ggaacaaaaa
    16741 tataacatac gaacggatga agtgacattt attttctttg atggaggaaa agaagcaaat
    16801 cggataactg cgggtaaggg aggaataact gattttgata aggagttgat ccctgaactg
    16861 aatcgacttc cgttagccaa tatcaaacaa ttagaagaag aattggatag aacgggagta
    16921 acttcccaaa attccgagat tgctaattga gacattggcg cgatgaaacg gtgggtctat
    16981 atagcgctat ttttaattat cagaacaagt gatgtttgat cttcaaaata acaaataaaa
    17041 aattaaaaaa tatagttgac gaaattcaaa aattcgatta gacttaaatc aattcttaag
    17101 gaaagggtga cctgtaatga acttatcaaa acgtatttca gcagaaaaca aaaaaaattg
    17161 gcgcagctgg cgtagataag ttgtattaat ggtgagactg tcccatagat acaacttttt
    17221 agagtgcatt tctaaagctt gtatctatgc tagacatgaa cattgactgc cgcatagatt
    17281 agaatctatg cggttttttg tgtaaagaca atgaccgctt tggccattgt ctttttttgt
    17341 agactggata cttgcctgat tataataaat aaaaagattg actttgcagg aggaaaaaaa
    17401 tgaaaaacaa acgattaata acagttgtag taattatttt agtttattta gcaggtgcat
    17461 ttatttatgg aaaagcctca ggcgaaaaag aaagtacttc gacgaaccag acaaaaaaaa
    17521 caacagttgt tggggtattg caatacgtga gccatccggc attagatgag atttatcgag
    17581 gaatcaaaga tggtctgaaa gaagaaggat taaaagaagg agaaaatctt gagatcaaat
    17641 ttcaaaatgg acaagccgat cagagcaagc tagccacaat gagtcaacag ctagtacaat
    17701 ctgatccaga tgccttgatt ggaatcgcaa cacctgccgc tcaatcatta gcaaacacga
    17761 cttcatcgat cccagttgtg ttaggtgctg taacggatcc tgtgggggct ggattagtcg
    17821 cttctttaga gaaacctggt ggtaatgtaa caggtgtttc tgatcagccg cctgttactt
    17881 cacaaatcaa gcttgcttct gatttgttgc caaatgcaaa aaaagtcgga atcttgtatg
    17941 cttcttcaga ggataattca aaataccaag tagcacaagc agaagaagct gcaaaaaaag
    18001 aagggttaga atcagtaaaa tatgcggtgc cttccactaa tgagattgca caaacggttc
    18061 aagtgatgag ccgacaagtc gattttatct atgttccatt agataatacc attgccaatg
    18121 cgatgcaaac agtggtaaaa gaagcaaaca aagcaaatat ccccgtcatt ccatctgtgg
    18181 atacaatggt ggaacaaggt ggactagcaa caatcgggat caatcaatat caattagggc
    18241 ttcaaagcgg taagatggcg gcagaacttg cgagtggaaa agaaaaaccg gaaacgacgc
    18301 ctgtttatac atttgatcaa ggggatacag tgatcaatca atctcaagct gatcgcttag
    18361 gaatcattgt tccccaatcg atgaaggaaa aagccaaaat cattacagat gactcgcagc
    18421 aagaaacatc taaggaggat gacaaatgat cgtatcagct atttcacaag gcatgctttg
    18481 ggcaatatta ggattaggga ttttcatgac ttatcggatc ttagactttc ctgatatgac
    18541 gacagaaggg tccttcccat taggtgctgc tgtctgtgtg acagctatta ctcatggggt
    18601 agcgccgatt gtagctacat tattaggcgt aggtgctggc atgatagcag gactcgttac
    18661 ggggttactt tatacaaaag gaaagatacc ggttatttta gcagggattt tagtgatgtc
    18721 aggattgaac tcagttattt tgtttgtgat gcgaacacca aatctttctt tattgaatca
    18781 tccgattcta caagacagct tccatcgctt tgggttacct gattactttg acacagtcct
    18841 tcttggcttc atctctttgg gcatcttgat tgctgtactg ctattcttct tcaatacaga
    18901 tttgggtcaa ggctatattg cgacaggaga caacgaaacg atggctcggt cgttagggat
    18961 aaaaaccgat cgtatgaaaa ttttaggttt aactttgtcg aacggtgtca ttgcattatc
    19021 cggtgcgctg attgcccaga acgacggata tgcagatgta aataaaggga ttggtgtaat
    19081 cgtcatcggt cttgcttcca tcatcatcgg tgaagtgatc ttccaagaat tgacgctagc
    19141 agaaagattg attgctattg ttgtcggcag tatcatttat cagctattga ttttagtagt
    19201 aatcaaacta gggtttgaca cgacttatct gaagttgttc tccgctatca tcttagctat
    19261 ttgtctgatg atcccgcaat tgaagaaagc actgaactta aaaacgggtt ttgagaagga
    19321 ggcatagtaa tgactgatat attgacaatc aaaaatggca gaaaagtcgt aaacaatgga
    19381 atgaatgaag agaaagtact attggatgaa attaacctta caatcagaga aggggacttc
    19441 atcactgtcc ttggcgggaa tggtgctgga aaaagcacac tgttcaatac gatcgctggt
    19501 acgcttcaac tcagtcaagg atcgatctct tttaaagatc agctgatcac aaaagattca
    19561 gaagaaaaaa gagcacgatt cctttctcga gttttccaag accccaagat gggaacggct
    19621 cctcggatga cagtagcaga aaatttgcta cttgcacaaa aaagaggtca aaaacggaaa
    19681 ttccgtttac gtaacttgaa aaatcaaaaa gaagattttt tcagactatg tcaagaagtc
    19741 ggtaatggcc tagagcaaca cttggatact ccagcgggtg aattatctgg tggtcaacgc
    19801 caagcattaa gcttgctgat ggcgacgatc acagctcctg agttactatt attagatgaa
    19861 catactgcag ctttagatcc taaaacagct aaagcattaa tgcgattaac aaatcagcgg
    19921 atcaatgaac aaaaattgac ttgtctcatg atcacgcatc gaatggaaga cgccttgaaa
    19981 tacggaaatc gtctgatcgt actccaaaaa gggaaaatcg ttcaagatat ttctgaaaaa
    20041 gaaaagcaac atttgacaat gggcgacctt ttgcagttct ttgaagaagt agtggattga
    20101 tttcgacaga aaaattcctt tcagtagaag gacttgaaaa ctcattgaca tcttcctttg
    20161 attcatccta tacttaaaac aataaacaat taaacatatt tgggtgtttt gtaggatcac
    20221 gtagtcaatg attttaacaa ttaaatagaa tattctttgc tctgacaagt aaacgatgag
    20281 aaaagaattt gctactttgc cacctagacg tttcgtactt tttcgtatca aacgctaagt
    20341 ggcttttttt attgaagtaa gaacaaagga ggaataagat gatttctgga caaaccaaat
    20401 tagctgggct ctttgctgat ccggcttctc acagtttatc cccattgatg cacaatactg
    20461 cttttgaagc aaacaacatc gatgcggtgt atttagcttt ccaagtaaat ccaatgaatt
    20521 tgaagcaagc agtcgaaagt attcgaactt ttgatatgtt aggtgtaaat ctttcgatgc
    20581 cgaataaaac agcggttatt ccttatctgg atgaggtaag ccaggaagcg caattgatcg
    20641 gagcagtgaa tacgatcgtg cacgaaaagg gtcgtttgat tggctataat actgatggtg
    20701 cgggcttcat gcaatccgtg caagaagctg ggatttcgat caaaaagaaa aaaatgactc
    20761 tactgggagc tggaggtgca gcaaaggcag tcactgttca agcagcgtta gacggcgctg
    20821 aggaaatcgt agtttataaa cgaaacaacc aaacattcgc tcaagtaaaa gaaacattcg
    20881 aacaactcag ctatcgaact tcttgtaaga taaaagttcg tgactttgcc gatacgcatc
    20941 aattaagaaa agacttgaaa gaaagctgtc ttctagtcaa tgcaacagat atcgggatgg
    21001 gaaagaaaat caatcagtca ccgctcttag atataagcat gatgcatgag aatctagcgg
    21061 tttttgattt gatttatgca cctagagaaa cacgtttatt aaaagaagca aaaaaagtag
    21121 gagcaaaagg atacaatgga cttggcatgt tgatctacca aggagcaatc gcgtttgaat
    21181 tatggacaaa ggaaaagatg ccgatccatg tgattgaaaa gctgtttgat taaaaaagga
    21241 taatagataa agatctttta ttctcgcata aacggtctca agaaaagtaa tcctttatgt
    21301 atcgaagcat ttatttcgtt attttttatt aaattatcaa aaaatgatag gcttttgtcg
    21361 attggttatc gtatccttta tttatcagta agaaacgagg aggaaccaca atgagtcgga
    21421 tggatcgcta taaggacatc cataagaagg cgaaacctct gaagcgggat acggggagta
    21481 tttttgctag aagagaacga aaacgaacgc gtatggaaaa tgaggaagat gctacccgta
    21541 tttatgaaag agagcagcat tcatttgaac atttgaacca atacgagcaa cagatataca
    21601 gagatgcacc aagagcagaa gaacaacaaa aaaataaaaa atttttcaat cggcaaaaga
    21661 agaacaaacc gttaaaacct cgaagaaagc gatcgtggaa aaaaatcatt ctatcgattt
    21721 tgcttgtttt gatcctttat tcaggtatct cattttttat tggtaaatat gtagctgaac
    21781 atgacacaag cttagaggca gcgacttcag aaacctttca tgggcaaaaa gcgacgaatg
    21841 gtgcgcataa tattttgatc cttggaagtg atacacgcgg agaagatgcc gggcgagccg
    21901 atacgatcat ggtcttacag ttggatggtc cttcccataa gccgaaactg atttctttta
    21961 tgcgtgacag cttcgttacg atcccagggg taggacaaaa taagatcaat tcagcttatg
    22021 catacggagg agccgatcta gtgcgtcaaa ctttagtaga aaattttgga atcgattgtc
    22081 aatactatgc gaaagtcgat ttcaaatcgt ttgaaaaagt gatcgatgcc ttattcatga
    22141 acggagtgaa gatcgatgca gaaaaagatc tgaatctgga tggtgtagat attaaaaaag
    22201 ggggccagaa gatggatgga catgttcttt tgcagtacgc acgtttccga atggatgaac
    22261 aaggagactt tggccgtgtc agacgccaac agcaagtaat gaacgctatt ttcagccaat
    22321 tgaagaatcc attgaatctg atccgtgcac cttatgcagc tggtaaagct ttaggatata
    22381 catccacaga tgtcactgct tggttcttga cgaaaaacct cttttcgatt gcacgaggag
    22441 ctggtggcat tgatcgaata agtgtgccag tagacggttc gtggaattat gggcaaagtg
    22501 attatgctgg cagtattctt gaaattgata atcaaatgaa tcaatctgct attacagatt
    22561 ttttgaataa atgatcataa aaaagatcaa gaagaagctg cgcaaggctt ttgtcatgaa
    22621 tcaatcaaac gattcactcg taaaaaacgc tttcttatgg tagatttaac caagagagga
    22681 gctgtttaca ttggatatct atctgaactt caaaaatgag tcgcgtgaag ccatcgcttt
    22741 ttatgaaaaa gtttttcaga ctgaagcatc aggtatcacc acatacggtg aaatgcctgg
    22801 aatagaacaa atgaacttgc cagaagaagc aaaaaatcta atcacgaatg cacggctgaa
    22861 catttatgat acattagtta tgtttgcaga tacgccagaa ggaattggtc ctgaattagt
    22921 tgtgggaaat aatgtttctt tagtcatcca gacaagaaat gaagaagaaa cgaagcgcct
    22981 atttgaaagc ttagctgagg gtggtaagat tgagatgcca ctggatgctg ttccgtgggc
    23041 tcagttgtat gggatggtaa ctgatcgatt tggcatcaac tggcagatta accaagagtg
    23101 acagctaaag atgaatattt tctggaaaca tcaagcagca aacggatcat cgtttgtttg
    23161 cttgatgttt ttgtgtctat ttcgactata cggtatcttt tttttattgc cagatgcgaa
    23221 aatatgttcc ataatagaga agtccgcaaa aaattaagta acggaggtca gaagatgaag
    23281 acggtgatac tagctgaaaa accttcacaa gcaaaagcct atgccgaaac cttccaacag
    23341 caaaagcggc atgaagggta ttatgaaatc agcgatccat tgtttgctgg tgaagtaacc
    23401 atcacttatg gttttggaca tttggtagat atggttgctc caggagctta tgaagagcgt
    23461 tgggcaaaat ggtctttaga aaatttgcct attttcccag aaaccttccg ttacgaagtt
    23521 ccaatggaca aacgtgcaca attttcgatc gtaaaacgag aattgcagtc agccgatacc
    23581 atcattattg caacagatgg tgatcgagaa ggtgaagcca tcgcgtggtc gattattcga
    23641 caagcaaacg cattttctaa ggaaaagaac tatttacgtt tatggatcaa ttctcttgaa
    23701 aaagaagcaa tctatgaagg ttttaagaat ctccaacctg gaaagaacta ttttccaaaa
    23761 tataaagaag cgcaagcaag gcagaacgct gattggctga ttggaatgaa tggaagtcca
    23821 ttatatagct tgctacttca acgaaaaggg attgatggca gtttttctct tggtcgtgta
    23881 caaacaccga cactctatat gatttatcag ctgcaagaag aaatcaaaca cttcaaaaaa
    23941 gaaacgtact ttgaaggaga aggtttaatt acgacaaaca aagggcagtt ttctggaaaa
    24001 atcgtaccaa aaaaagcatt taaaacccaa gaagagttaa cgaaggaaat agaaaaactt
    24061 ggagcgcatt taggtaagca aaacggacgg attcttgaag ttacgaaaaa ggaaaagagg
    24121 ttgcatagtc cacgtttatt ttcgctatct agtttgcaga caaagatgaa tcagctaatg
    24181 aaagcaagtg ccaaagctac acttgatgct gctcaaggat tgtacgaaag caagtttcta
    24241 agctatccaa gaacggattc cttttacatt acagaaaatg agcatcagta tctggttaaa
    24301 catctgcaaa attataaaaa atttttagga atagaagaag tacagacaac tgagatcaaa
    24361 gcaaagaaac gatatgtcga tgcgaaaaaa gttcaagaac atcatgcaat tattccgaca
    24421 aagacgattc cgactcctgc acgctttgcg aagctttcca aattacagca agcaatctat
    24481 ttgcaagttt tgcgcactac tgtggctatg tttgctgcag attatatcta cgaagagacg
    24541 accgtaatga cgggcgtaca gcaattaaag ctaaaaagca tcggaaagat cccattacag
    24601 caaggttggc aggaaatctt acgcatcaag tcaaaaaaga aagaagctca gactcttcct
    24661 cctatcgtag aaaatgaaga agcagaagta gaactgaaaa gtatggagaa agaaacacaa
    24721 cctccaaaac cctataccga aggaacgttg atcacagcaa tgaaaactgc gggaaaaacg
    24781 ctggataatg atgaagcgca agaaattctc aaagaagtag aaggaatagg gacagaagct
    24841 acacgagcca atatcattga aaatcttaag cagaagcatt acatcgaagt caataaaaac
    24901 gaaataactg ttacgccaaa aggaatcact ctttgtaagg cagtagccaa tgaaccatta
    24961 ctgacgagcg cagaaatgac tgcgcgctgg gaagggtact tgagaaaaat tggtaaacaa
    25021 gaggggacac catctgtttt tttagagaat atcaaaaaat tcatcctaca tttattggca
    25081 gaagttccta atcaaatcga caaggtagac ttttcagatg agattagcgg gacacgtcag
    25141 ataaaagcga tggagaagaa aaacgatcaa ttaggaagat gtccaaaatg taaaaaggga
    25201 atagtcatgc tttatcctaa aatcgctaca tgcttgaatc cggaatgcga cttcaaattg
    25261 tggccaacag tagctaaaaa gaagctgact aaaacgaatc tcagagattt gctttcaaaa
    25321 ggaaagacaa gtaaagttgt caaaggtttt actggtaaaa aagggaaatt cgatgcagtt
    25381 ttagtattaa aagaagatat gacaatcggt ttttcttttc catggatcga aaacagtgga
    25441 acaaaagcag ttgatggaaa caaagaaaca tcaagaaaaa ataatgaaaa cgaagtagag
    25501 taaatgaaaa caatgaaata gcggaatatt tattccgaaa agtgagaata taaataccaa
    25561 taaaactaaa aaatatcagt gaatcaagca aaaacgttgg ggtaggctga ttttatatag
    25621 atagcaagga gtttttttat ttgaaaaaag tgcattcatt tttaaattat cttatgggag
    25681 caatcacgat tgtggctgtg taccaagcat ttctaaagta tcctgattac gggacgaagt
    25741 tgttgtcatt gacgcacaat caaacagcat attcaccaac gttgatttta tcgactttga
    25801 ctgcaggagc taaaagcgct acttattttt ttcataaaac gacaagcatg ctaaagcgaa
    25861 agaaacagcg aaaacaattg ttggtggtgg tagcggctat gatcattatt tattttctac
    25921 cagttattac acaagtattt catcaatatt ttggttaaga atacgagagt ttgtgacaaa
    25981 agtcatgtca caaacttttt tctttgtcat tttttcgtaa gaaaaagcaa aataaaagta
    26041 tatacattga ttaaaagtat atacttggat ataatgtact tactatagaa tgagaatggt
    26101 atagagggtg gcgaaatatg aaaaaaatga aaaagtataa aattagaaaa tctggcaata
    26161 gcgatgtaac aactatccca ccagaagtaa aagaattcat gggtgtgcag acaggtgacg
    26221 caatcagtta tgtttttcaa tctgatggtt ctgtacgaat gatcaaagca caagaagaac
    26281 ctgatattga ttcattagtt gattcgatca tgaatcaata tgaagacgcg ttaaaagatt
    26341 tggttgattt atgaggtatc tttcgactaa agaaattatc aaaatcaacg cgaaagttat
    26401 tttacgtcac tcgccaggcg aaatgatcgg tgtaaaagat gcaaatgcat tagatatggc
    26461 cgtcaaacag ccttcacaag cagtattcaa tcaagaactc tatcctgaaa tctatgaaaa
    26521 agcggcgatt ttagcaatta atttagctaa aaaacatcct tttcataatg gcaataaacg
    26581 aactgctctt gttgccatgt tgttgtttct tcaattgaat aattgtccag ttgctttttc
    26641 tcgtcaagaa gctgttgatt ttatcataat gattaccact agttcattgg atttcgattc
    26701 actaaagtcc aagataacca gttatttacg acaaaccgca ataaaatgaa cgaattaaaa
    26761 actttatttc tcaattagat cagcatg
//
LOCUS       DAGCXG010000027        25808 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGCXG010000027 DAGCXG010000000
VERSION     DAGCXG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.028"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(11..556)
                     /locus_tag="MB022_001197"
     CDS             complement(11..556)
                     /locus_tag="MB022_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4466207.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(568..1260)
                     /locus_tag="MB022_001198"
     CDS             complement(568..1260)
                     /locus_tag="MB022_001198"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBT4466208.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1279..1563)
                     /locus_tag="MB022_001199"
     CDS             complement(1279..1563)
                     /locus_tag="MB022_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466209.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1576..2139)
                     /locus_tag="MB022_001200"
     CDS             complement(1576..2139)
                     /locus_tag="MB022_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4466210.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2367..3029
                     /locus_tag="MB022_001201"
     CDS             2367..3029
                     /locus_tag="MB022_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBT4466211.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3153..4349
                     /locus_tag="MB022_001202"
     CDS             3153..4349
                     /locus_tag="MB022_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBT4466212.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4450..5826)
                     /locus_tag="MB022_001203"
     CDS             complement(4450..5826)
                     /locus_tag="MB022_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4466213.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5863..6729)
                     /locus_tag="MB022_001204"
     CDS             complement(5863..6729)
                     /locus_tag="MB022_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBT4466214.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6818..7843)
                     /locus_tag="MB022_001205"
     CDS             complement(6818..7843)
                     /locus_tag="MB022_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4466215.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7861..8535)
                     /locus_tag="MB022_001206"
     CDS             complement(7861..8535)
                     /locus_tag="MB022_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4466216.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8719..9612)
                     /locus_tag="MB022_001207"
     CDS             complement(8719..9612)
                     /locus_tag="MB022_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4466217.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9686..11383)
                     /locus_tag="MB022_001208"
     CDS             complement(9686..11383)
                     /locus_tag="MB022_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBT4466218.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11376..12689)
                     /locus_tag="MB022_001209"
     CDS             complement(11376..12689)
                     /locus_tag="MB022_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBT4466219.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12689..17197)
                     /locus_tag="MB022_001210"
     CDS             complement(12689..17197)
                     /locus_tag="MB022_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBT4466220.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEGELPLQVVV
                     DERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSGQALQRIQRQLFNGILER
                     LGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDG
                     ENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYN
                     GAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASM
                     SELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSD
                     IFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVV
                     GDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFT
                     HPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAI
                     NTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTP
                     RYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPG
                     ENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQV
                     KLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQ
                     NGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWI
                     REIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYY
                     EGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFEL
                     YETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPD
                     SFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENW
                     QYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLY
                     RAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNG
                     YRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLEL
                     LKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKE
                     VKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQ
                     QELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVR
                     QSKKEGAR"
     gene            complement(17495..18013)
                     /locus_tag="MB022_001211"
     CDS             complement(17495..18013)
                     /locus_tag="MB022_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4466221.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18015..18632)
                     /locus_tag="MB022_001212"
     CDS             complement(18015..18632)
                     /locus_tag="MB022_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4466222.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18629..19483)
                     /locus_tag="MB022_001213"
     CDS             complement(18629..19483)
                     /locus_tag="MB022_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4466223.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19480..20391)
                     /locus_tag="MB022_001214"
     CDS             complement(19480..20391)
                     /locus_tag="MB022_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466224.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20432..21766)
                     /locus_tag="MB022_001215"
     CDS             complement(20432..21766)
                     /locus_tag="MB022_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4466225.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21974..22804
                     /locus_tag="MB022_001216"
     CDS             21974..22804
                     /locus_tag="MB022_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBT4466226.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23048..23366)
                     /locus_tag="MB022_001217"
                     /pseudo
     CDS             complement(23048..23366)
                     /locus_tag="MB022_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23488..23682)
                     /locus_tag="MB022_001218"
     CDS             complement(23488..23682)
                     /locus_tag="MB022_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466227.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24187..24372)
                     /locus_tag="MB022_001219"
     CDS             complement(24187..24372)
                     /locus_tag="MB022_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466228.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25222..25563)
                     /locus_tag="MB022_001220"
     CDS             complement(25222..25563)
                     /locus_tag="MB022_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4466229.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25563..25793)
                     /locus_tag="MB022_001221"
     CDS             complement(25563..25793)
                     /locus_tag="MB022_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4466230.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         7630 a         5534 c         4421 g         8223 t
ORIGIN      
        1 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
       61 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
      121 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
      181 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
      241 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
      301 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
      361 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
      421 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
      481 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
      541 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
      601 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
      661 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
      721 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
      781 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
      841 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
      901 ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc
      961 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
     1021 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
     1081 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
     1141 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
     1201 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
     1261 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
     1321 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
     1381 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
     1441 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
     1501 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
     1561 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
     1621 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
     1681 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
     1741 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
     1801 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
     1861 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
     1921 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
     1981 agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc
     2041 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
     2101 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
     2161 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
     2221 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
     2281 ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
     2341 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
     2401 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
     2461 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
     2521 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
     2581 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
     2641 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
     2701 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
     2761 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
     2821 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
     2881 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
     2941 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
     3001 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
     3061 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
     3121 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
     3181 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
     3241 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
     3301 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
     3361 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
     3421 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
     3481 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
     3541 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
     3601 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
     3661 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
     3721 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
     3781 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
     3841 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
     3901 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
     3961 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
     4021 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
     4081 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
     4141 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
     4201 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
     4261 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
     4321 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
     4381 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
     4441 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
     4501 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
     4561 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
     4621 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
     4681 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
     4741 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
     4801 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
     4861 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
     4921 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
     4981 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
     5041 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
     5101 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
     5161 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
     5221 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
     5281 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
     5341 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
     5401 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
     5461 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
     5521 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
     5581 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
     5641 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
     5701 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
     5761 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
     5821 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
     5881 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
     5941 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
     6001 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
     6061 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
     6121 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
     6181 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
     6241 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
     6301 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
     6361 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
     6421 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
     6481 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
     6541 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
     6601 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
     6661 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
     6721 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
     6781 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
     6841 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
     6901 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
     6961 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
     7021 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
     7081 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
     7141 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
     7201 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
     7261 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
     7321 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
     7381 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
     7441 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
     7501 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
     7561 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
     7621 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
     7681 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
     7741 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
     7801 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
     7861 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
     7921 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
     7981 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
     8041 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
     8101 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
     8161 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
     8221 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
     8281 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
     8341 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
     8401 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
     8461 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
     8521 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
     8581 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
     8641 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
     8701 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
     8761 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
     8821 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
     8881 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
     8941 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
     9001 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
     9061 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
     9121 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
     9181 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
     9241 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
     9301 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
     9361 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
     9421 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
     9481 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
     9541 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
     9601 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
     9661 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
     9721 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
     9781 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
     9841 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
     9901 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
     9961 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    10021 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    10081 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    10141 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    10201 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    10261 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    10321 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    10381 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    10441 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    10501 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    10561 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    10621 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    10681 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    10741 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    10801 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    10861 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    10921 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    10981 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    11041 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    11101 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    11161 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    11221 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    11281 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    11341 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    11401 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    11461 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    11521 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    11581 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcatc catctcctgt
    11641 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    11701 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    11761 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    11821 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    11881 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    11941 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    12001 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    12061 atctcttgca gttcctcgct tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    12121 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    12181 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    12241 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    12301 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    12361 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    12421 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    12481 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    12541 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    12601 tcttctgccg acatcgcctg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    12661 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    12721 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    12781 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    12841 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    12901 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    12961 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    13021 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    13081 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    13141 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    13201 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    13261 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    13321 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    13381 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    13441 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    13501 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    13561 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    13621 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    13681 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    13741 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    13801 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    13861 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    13921 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    13981 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    14041 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    14101 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    14161 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    14221 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    14281 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    14341 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    14401 ggaattgata cgtctcatat actaactgat aacgtggtgg tccagcgatc tctctgatcc
    14461 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    14521 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    14581 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    14641 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    14701 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    14761 tcactgtgta aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    14821 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    14881 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    14941 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    15001 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    15061 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    15121 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    15181 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    15241 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    15301 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    15361 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    15421 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    15481 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    15541 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    15601 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    15661 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    15721 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    15781 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    15841 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    15901 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    15961 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    16021 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    16081 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    16141 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    16201 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    16261 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    16321 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    16381 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    16441 tatagtcatt cgttgtaaat ttcgggtagc gatgctgttc gttttcaact tctccgctag
    16501 gatagatata ttcatagggg tcttgcgtag ctgttggccc aagacgctcc aaaataccgt
    16561 tgaataactg gcgttgaatg cgctggagtg cttgaccgct gggtacgatt ggttcgtcca
    16621 gcttttccat ctcattttct tcgctacgtt gaatttcagc ggaatcttct gtatcagttt
    16681 cattttcttg taattcttgg ttatctattc tttcatcaac caccacttga agaggtagtt
    16741 ccccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    16801 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    16861 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    16921 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    16981 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    17041 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    17101 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    17161 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    17221 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    17281 ttttaatttt aaatcaaatg atatcctttt tagttaaaaa acaccaatta tacaacattt
    17341 attattaaaa ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt
    17401 aagacaaatg aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc
    17461 atttacgcac tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc
    17521 caataacact tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa
    17581 aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac
    17641 ttaattcgaa caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag
    17701 aagaagtctt cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt
    17761 tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga
    17821 taagaatggc tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga
    17881 gtaaccaaat aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc
    17941 gactatccgt tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa
    18001 tcaaacgcaa catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa
    18061 agcaggactt gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct
    18121 gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg
    18181 aaatcattaa aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga
    18241 agcaagattt tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac
    18301 atcaatgtat agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga
    18361 agaagaaaat aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga
    18421 aactttttcc agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc
    18481 atatacagaa ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac
    18541 catagtagct gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca
    18601 ttggcttttt taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc
    18661 cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg
    18721 ttgctcctgc catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc
    18781 caatctgtaa cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat
    18841 tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa
    18901 gcatcaaatg actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca
    18961 attcattatt gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga
    19021 agaacgatgg aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata
    19081 catattgagg gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa
    19141 acatcagctt tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg
    19201 aaaggacatt accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc
    19261 aaaaaggaaa ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa
    19321 aggtcggagg ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa
    19381 gcaatggaat gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt
    19441 ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt cacctccaga
    19501 ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag
    19561 aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt
    19621 ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt
    19681 catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta
    19741 aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc
    19801 tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta
    19861 aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca
    19921 ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat
    19981 caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa
    20041 gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag
    20101 tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata
    20161 aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt
    20221 tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac
    20281 tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa
    20341 taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg
    20401 cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta
    20461 ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt
    20521 caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca
    20581 ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt
    20641 tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt
    20701 cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct
    20761 caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc
    20821 ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat
    20881 tttccatcaa actagagtaa taattgatat tttgggcacg catggctact aatccggaaa
    20941 taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc
    21001 cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa
    21061 tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata
    21121 catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt
    21181 ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt
    21241 cactgattcc agcttcttta aataactttt tattataaaa aatgacttgt gtatatgtat
    21301 tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa
    21361 aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg
    21421 ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta
    21481 ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga
    21541 tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac
    21601 cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag
    21661 aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa
    21721 acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa
    21781 ttttaatgta accgatgtcg gttttaatat aacgatatca ttttgttctg aaaataacaa
    21841 tttataaggt atgattctaa aaaaattatt aatgatacca atactttata taattcgcaa
    21901 gcgttttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
    21961 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
    22021 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
    22081 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
    22141 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
    22201 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
    22261 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt
    22321 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
    22381 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
    22441 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
    22501 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
    22561 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
    22621 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
    22681 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
    22741 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
    22801 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
    22861 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
    22921 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
    22981 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
    23041 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
    23101 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
    23161 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
    23221 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
    23281 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
    23341 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
    23401 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
    23461 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
    23521 ttcaagaagt ccaagaaata aagccgtata aaaaccagaa cctatgaaat ctttatttat
    23581 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
    23641 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
    23701 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
    23761 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
    23821 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
    23881 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
    23941 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
    24001 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
    24061 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
    24121 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
    24181 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
    24241 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
    24301 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
    24361 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
    24421 aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac
    24481 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
    24541 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
    24601 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
    24661 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
    24721 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
    24781 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
    24841 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
    24901 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
    24961 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
    25021 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
    25081 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
    25141 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
    25201 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
    25261 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
    25321 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
    25381 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
    25441 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
    25501 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
    25561 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
    25621 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
    25681 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
    25741 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
    25801 actccctt
//
LOCUS       DAGCXG010000028        25677 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.029, whole genome shotgun sequence.
ACCESSION   DAGCXG010000028 DAGCXG010000000
VERSION     DAGCXG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.029"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(182..616)
                     /locus_tag="MB022_001222"
     CDS             complement(182..616)
                     /locus_tag="MB022_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466231.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(726..2585)
                     /locus_tag="MB022_001223"
     CDS             complement(726..2585)
                     /locus_tag="MB022_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4466232.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(2703..3863)
                     /locus_tag="MB022_001224"
     CDS             complement(2703..3863)
                     /locus_tag="MB022_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBT4466233.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(4170..5594)
                     /gene="araA"
                     /locus_tag="MB022_001225"
     CDS             complement(4170..5594)
                     /gene="araA"
                     /locus_tag="MB022_001225"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBT4466234.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(5610..6308)
                     /locus_tag="MB022_001226"
     CDS             complement(5610..6308)
                     /locus_tag="MB022_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4466235.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(6265..7926)
                     /locus_tag="MB022_001227"
     CDS             complement(6265..7926)
                     /locus_tag="MB022_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBT4466236.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            8144..9544
                     /locus_tag="MB022_001228"
     CDS             8144..9544
                     /locus_tag="MB022_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBT4466237.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(9796..10875)
                     /locus_tag="MB022_001229"
     CDS             complement(9796..10875)
                     /locus_tag="MB022_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4466238.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(10961..11920)
                     /locus_tag="MB022_001230"
     CDS             complement(10961..11920)
                     /locus_tag="MB022_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBT4466239.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(11932..13443)
                     /locus_tag="MB022_001231"
     CDS             complement(11932..13443)
                     /locus_tag="MB022_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4466240.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(13465..14028)
                     /locus_tag="MB022_001232"
     CDS             complement(13465..14028)
                     /locus_tag="MB022_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4466241.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(14034..14897)
                     /locus_tag="MB022_001233"
     CDS             complement(14034..14897)
                     /locus_tag="MB022_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4466242.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(14911..15807)
                     /locus_tag="MB022_001234"
     CDS             complement(14911..15807)
                     /locus_tag="MB022_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466243.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(15982..17271)
                     /locus_tag="MB022_001235"
     CDS             complement(15982..17271)
                     /locus_tag="MB022_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4466244.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(17243..18760)
                     /locus_tag="MB022_001236"
     CDS             complement(17243..18760)
                     /locus_tag="MB022_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4466245.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            18958..19848
                     /locus_tag="MB022_001237"
     CDS             18958..19848
                     /locus_tag="MB022_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBT4466246.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(19921..20745)
                     /gene="nadE"
                     /locus_tag="MB022_001238"
     CDS             complement(19921..20745)
                     /gene="nadE"
                     /locus_tag="MB022_001238"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBT4466247.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(20757..22229)
                     /locus_tag="MB022_001239"
     CDS             complement(20757..22229)
                     /locus_tag="MB022_001239"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBT4466248.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(22866..23228)
                     /locus_tag="MB022_001240"
     CDS             complement(22866..23228)
                     /locus_tag="MB022_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4466249.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            23451..24203
                     /locus_tag="MB022_001241"
     CDS             23451..24203
                     /locus_tag="MB022_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4466250.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(24343..25266)
                     /locus_tag="MB022_001242"
     CDS             complement(24343..25266)
                     /locus_tag="MB022_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4466251.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(25281..25553)
                     /locus_tag="MB022_001243"
     CDS             complement(25281..25553)
                     /locus_tag="MB022_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466252.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         7499 a         5381 c         4337 g         8460 t
ORIGIN      
        1 aaaaccgccg agatggttag actcgacgga aaaggagtat ttactcgatt ttgagtaagt
       61 acaaggttgt tgttggttat aggtatgtca ttatcataaa ggattttgat cactctatca
      121 taaaggtaat attacattta tattagataa ctctagtttt tattgttatc aaagatttct
      181 tttatttttt caacaacaaa ttagtcattt tttggatggc agctgtcaat tggtctgtcg
      241 cttggtcttg ttttgcttca gatgtattga atagatcttc atctacccct gattcaagat
      301 cgatactttg gatcatttct tcacagcctg ctacatatga aagataagct ttttcaaaat
      361 ttttatgaat gcccattgct tttgctggtg gacgcaaagt actgactttc ttaagcattg
      421 cccgatattt ttttgttcct tcttcaaata acaggatgat ctcttcaaga gtcgatttgt
      481 ccagttcact tgtttttcct tcatcaattg cgtttctgat cacttcatat ttcggatgca
      541 ttccttctcc gattgcttct gtttccttga cgatttgatc gatggactgc acataatagc
      601 caatattcgg taacatgttc gttttcctac tttctccatt gttgttatta tttattctac
      661 ttgtttttat cctcaaatga caagtacttg aaaaaattta atggtttcta tacagctcgc
      721 cattcttatt cgtgctcaaa gaaaccatct gctaaactat attgtttccc tgcgacaatg
      781 tcatattgga tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct
      841 tctttatacc actgtccgtt ttgatagtac agttctcgtt caaacgccgg aagttcctgt
      901 tctaatgata aaagcagctg ataaaagcca tttgttgtct gattcgtctg ttccattaga
      961 ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag tcgtttgctt ttctaattct
     1021 cctttactgt cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc attttttgct
     1081 tggatcgtat ctgaatagat ccctggaaga tgatcccccc aaaaaacaac taaagtcctt
     1141 ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata
     1201 tcttgcagat aattctctaa gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag
     1261 tctagattat catactttcc atcgtagggc atatgtgtct gcatcgtgac taaatgaatg
     1321 aactgaggtg ttttttcttc cttgagaagt gtcattactt ctttgtaagc tgactcatca
     1381 gaaatatatg gattattctc tatcgtatcc gtataggtca tcgtacgctc gctgatgaac
     1441 tggtcaaatc ctaatgtctg ataaacatcc tctcttttat acatcgaagt attgtatgga
     1501 tggatcgctg tcgtctggta tgattgtgcg tttaacgctg aaacgataga aggaagctga
     1561 tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata
     1621 gaaaaaccag tcaatgcttc aaattcgata tttgctgttc caccgccata gttttgagaa
     1681 agcatattgc cactatatgt ttgatcagct acttcataat aatctgccaa tggctcacca
     1741 gatacttcta cgccgtttaa tcgactagga tctgagaaac tttcactcat cacgaacacg
     1801 atgttcggcg actcttcctc tacagtttta tttttttcat cggctagctt ctggtatttt
     1861 tcagtgattt ctttgatctt ttctttcgaa tatccttcag gttcatccat tggttctacc
     1921 ttcagattgt acaaaaaccc accgataaaa ccagtgttat agtagttcat tttctggctg
     1981 taaggtatcc atagtgccgt tttattatat gcttttcgca acaaattatc gggattgttg
     2041 aaattactga tataccctag cagtccaatt gccacaagca gtgtcgttaa tcggatcaat
     2101 tggaatcgcc ttcctttttt caggcttcga aacatgtacc agcaaaacaa tcctatcacg
     2161 gttcctgcgg cagctaagat cactgtaaac agcatagtgc cggtcatatc tttcagcaat
     2221 ccgatttcag tgatcatctt caaatcgtct ggataaatcg gttcttgccg ataactcatt
     2281 ttcatataat ttgcaaaacc taatacaccg atggctacga catacgtaag ggaacctaaa
     2341 gggacagatc ctgctagtgc aatcagaaaa ataagaataa tcagcaagac gagacaggct
     2401 aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc tacggataga
     2461 tcattttgac accactgcaa aaataaatta ctaaaaacaa caagaaaaaa gatactgatg
     2521 attgctgctg ttccttttaa aatctttttc tgtttggtcg aaagtctgct tatatatctt
     2581 ttcaaatgct tgctccttca tttctatcaa taaaagaatg ttgcttctct tattttagtt
     2641 gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa tattctcttt
     2701 ctttatggcc tttctggttt ctttaataag cggacctttc gtactccaga aattttttct
     2761 agctgttgtt tcacttgatt gagctgttca aaatcagtac tgtctatgtc aatcaatatg
     2821 tagacatgct cattcttatg gttacgcgat atatctgcga tcgctagctc gttctcattc
     2881 agcagagaaa atatctctgc taatatattg gttttctttt gataaaatat agtgaatcga
     2941 aacggcgctt ggaaaaacat tcgtgcggat ggataattga ccgcttctct aaccgtaccg
     3001 aacaaaagat aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc
     3061 tgagtcgatc cccctagatg aggcaacata agtatacggt cattttctaa gaactcttct
     3121 tctggaaaat cagtcaagta tttagccaga tgttgttttt ccaatgcttg cataacagca
     3181 tatttatcaa caatttcact tcttcctaca ttgataagta cagcatcttt tttcattttt
     3241 tctatattcg cctgatcgat caatcctttt gtatcttctg tcaacggcag catgactacg
     3301 atgtaatccg aggtagaaag aactgtttct aaatctgctt gccgaacata atccaactgg
     3361 gcatcgcgga tagaataacc caagacatcc atccctaagc tatagcaact tagcgctact
     3421 tttgtgccta tcgctccaag accaagaaga ccgatggttt tgccttgcaa ttctcgtccc
     3481 acataagcac ttcttttgtt ttctgcctgt tctaaaatat taggacctgt tagtgtttgt
     3541 accatacgac ttgcttcgat tatcggacgg gaagataaga gcaaacagca caaaacaagc
     3601 tcttttactg cattagcatt tacacctggt gtattcatga cgatcgtccc gttttcagaa
     3661 gctttttcaa cgttgatcgt gttcacccca acaccagctc ttgaaattgc tagcaaatct
     3721 ggtgtaagcc attcatcttt cactttcgta ctacgaataa ctattccttc ggctggcaaa
     3781 tcatctatta ttttgaaata ttccccttgt aacttttcta tctcttgttc tggaatagca
     3841 tcaatcaaat gaacgtgata cataaaaccc cctccttact aacaagtatc tcgaaatttc
     3901 tgcaattaga caactgttta ccatttgact tcctagaagc aaaaagagca gttagagcag
     3961 agtaggctga gtgataaaaa taaggctatg acaagttact gccataagcc ttattttcga
     4021 gagcaaaata ttgagggtga gatatcttga taaatggtgt tccgttagct gacattgata
     4081 ataagactaa atttcaaaaa agttgaaaag caaatttcgt caatctcttc ttatttctta
     4141 aaactaagtg gctttttcac aacctctttt tatctaataa ccactgtttc caactcaact
     4201 aattttgccc acgtttcaat ttgatctgtt gttagattta gcgagacaac ggtatgatga
     4261 ccgccacctg cctgaatcca tgaatggaca ccttcatgga aattcggttt agttttccat
     4321 aacacacgtg caacaggtaa attaggtgct tcagtcgttg gttcaaaggc ttctacttca
     4381 ttgatcagca gtttataatg cgtaccgaaa tctgccatcg atacgactac cccttctcct
     4441 gctttcccat caaatacaag acgcgccgga tcttctctat ctcccattga taaaggagaa
     4501 acaacgattc ttggtttcgt cgcagcaagc gtcggatcta cttccatcat atgggattcc
     4561 aagattgctt cttggccact agctagctcg taagtataat cttccatgaa tcctgtatct
     4621 ttccctcgag ccatgatttt cagcaaccga tcaatcgctg cagttttcca gtcgccttcc
     4681 ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag ctagtccagg tagttgtttc
     4741 attccataca ggtcttcaaa attagtcgta aaagcgttat agcctccttc ttctaagaaa
     4801 cggcgaatac cgatttcctg ctgagcttgt acttttacat gttcctccca tgtcttttct
     4861 tcataatcac catagtcgaa ggtatacaat tccttgtact cttcaaataa ttgatcgact
     4921 tcttcgtctg atacgcgatc gattacttgt acaagatctc cgataccaaa ataatcaaca
     4981 gtccaaccaa attgaatctg tgcttccact ttgtctcctt ctgtgacacc cacgtttctc
     5041 atgttgtcac caaaacgagc cacttttatt ccaaaacttt cattataggc tactgcaaca
     5101 tccatccaat cagcaatctc tttttggatc tcaggtcgtt tccagtagcc aacgacgatt
     5161 ttattttgtt tatttaatcg agcattgatg aacccatatt cgcggtcacc atgagcggat
     5221 tgatttaaat tcatgaaatc catatcgatc gttttccaag gaatgctttc gttatattgc
     5281 gtagctaaat gaagcaatgg tttttgtaga agctttgtcc cgcggatcca catttttgct
     5341 ggtgagaaag tatgcatcca agtgatcaca cctgctactt cttctcgata attgacctct
     5401 ttcatgattt ttgtgatcgt atcagcagta actgctaatt cttgaaggcg gattggataa
     5461 ggcaattggc cattttcatt cagttcatcg accatttctt gtgcatgttt ctttacttct
     5521 cgcagtgctt cttccccata taaatgctgt gatcctacaa caaaccaaaa ttctttttct
     5581 ccaatattca acataattag ccactccttt tatttgtttt gtccgtaata cgcatttttt
     5641 ccgtgttttc gtaaataatg tttatctaaa atccgttgag gcagctcttc tgaaaatgaa
     5701 ttgagctgtc tggtaaataa attcatcttg catacttcat ctagtacgac tgcgttcatt
     5761 accgcacttt gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa aagtacccct
     5821 ggaattgcta agggatcaat tttgcgttct ttgaatgttt ctatgataac ttttcctgtt
     5881 tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat
     5941 ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc
     6001 cagatcgtcg cccatgtcga gtgagtatgg acgattcctc cgatttgagg aaatgaacga
     6061 tataaaacag catgagtagg cgtatccgaa gagggattga gtttaccttc aatgatatga
     6121 ttgtccaagt ccacaacgac catgtcgtct gctgtcatct cttcataact gactccgcta
     6181 ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat
     6241 ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt atacttcttc tttcagttgt
     6301 tccagcattt tctatcactc ctttttcttg caattgatga atcgctgttt tttcaatgtc
     6361 gatcccctca cggtaacgtc tcataaatat ctgatagcct gctatttctt ctgctgaagg
     6421 tgtgatggtg attccttggt cttgtccaaa cacgtcttct gccaagaagt tttctaaaga
     6481 cttcccatgt cgatccatca tataggctgc taataacgcg attccccacg ctccaccttc
     6541 tccggctgtg tccatcactg taactggtgc ctccatagca gatgctagaa tccgctgtcc
     6601 aacttctggt gttttaaaaa tccccccatg ccccactaaa cgatcaattt ggacattctc
     6661 agatttcaat atatccattc cgattctcat tgcaccaaaa gcactggcta aatggatccg
     6721 catgaaattc gctaaatcaa aacggctacc tggtgtacgt acaaataatg gccttccttc
     6781 atttactcct gtaatgtttt caccggaaaa ataaccataa gaaagtaatc ccccgcaatc
     6841 cgtatcgcct tctagcgctt tgttgaataa taccgtgaat aatttatctc tgcttatctc
     6901 catacccaaa gaattggtaa attcttcaaa aatactgacc caagcattga tatccgaaga
     6961 gcagttattc gtatgaacca tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat
     7021 ttccggatgg acattttcta gttctttttc taatacgatc atagcgaagg cggaagtacc
     7081 agccgaaatg ttccctgtcc gtttggctac actgttcgta gcgaccatac cggttcctgc
     7141 atccccttct ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag
     7201 ataagcccct gattcgctta acttgccagc ttcttctcct gctactagaa ctttaggcaa
     7261 aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa aaatattttt
     7321 catttcttga tggtaggttt tcgttgaagg atcgatagga aacatgcctg atgcatctcc
     7381 tacgcctagt actttctcac cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa
     7441 aaaattaatt tctttcacat gttcttcttt attcaaaata gattgataca aatgagcgat
     7501 actccatctc tgcggaatat tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc
     7561 agtgattgta tttcgccaag tacgaaatgg tactagtaac tgttccttgc tatcaaatgc
     7621 tagataaccg tgcatcattg cactgaaccc aatcgacccg attgtcgtaa gattctcgcc
     7681 atagatttcg aatacttctg ctgccatttt tcggtaactg acgcgcagtc ctttccaaat
     7741 atcttctaat gaataggtcc agataccatt ttctagtttg ttttcccact cataactacc
     7801 agcggcaata ggagtaaaag atgaatcaat caatacagct ttgatcctag ttgatccaaa
     7861 ctcaatccct aaagatgtac gaccatctat gacatctaac atactttgcg catttacttc
     7921 cttcataatg atttaactcc ctccttctcg tcaaacctga tcaaaccgtc tttttagtag
     7981 cgcttacaat gattattgta tacttttgta cgtacatgtc aagcggttat ttacaagaat
     8041 acaataaaaa acacttgttt tgtacgtaca taagttataa aatgaaattg cttacaaaat
     8101 ctgatcaacc gccttctcga aattctagaa ggagagaata cttatgaaaa aagaaaaaaa
     8161 agtttcctca tcgtttattt atttttttgg cgcatttggc ggcattctat ttggatatga
     8221 tattggtgtt atgaccggcg ccttaccttt tttacagcat gattggggtt tggcaggtaa
     8281 agcttctttg ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc
     8341 tttatctggt cttctttctg ataagcttgg acgacgaaaa atgattcttc tttctgcact
     8401 tatttttatg gctggttctg tcctttctgc atctgcccct cacaatggtt cttattttct
     8461 gattgcagca aggattcttc taggtttagc tgttggcgct gcatctgccc tagttcctgc
     8521 ttatatgtct gaaatggctc ctgctagact aaggggaaga ttgtcaggga tcaatcaagt
     8581 gatgattgct tctggcatgc tcctttccta tgttgcagat tatttgctaa aagtgttgcc
     8641 tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc tgattttatt
     8701 tttcggtgtt ttagctctac cagagtcacc gcgttttctt atgcaatctg gccgcttgga
     8761 agaggcaaaa agagtattga actatattcg gacaccaaaa gaagctgaac aggaattcga
     8821 acaaatccag ctgaatgtca aacaagaaaa gacaaccgga acttcttggc atactttgtt
     8881 tttagaaaaa tatcgttcct tagtcattgc cggtattggt gtagcagtat tccagcaatt
     8941 tcaaggagct aatgctattt tttactatat tccgctgatc gtagaaaaag cgactgggca
     9001 tgcagcgagc gatgcattga tgtggccgat catccaagga atcattttag ttgctggttc
     9061 tttgctcttt ttggttattg cagacaagtt caatcggcga actttattga aaataggcgg
     9121 tagcgtgatg ggactttcat tcatcctacc agctgtttta ggaactgtac tggatgctca
     9181 tacaaattcg ttgttgattc tcttgttttt atgtatttat gtcgcatttt attcttgcac
     9241 atgggctcca ctgacatggg taatcatagg cgaaatattc ccactagctg tccgcgggcg
     9301 agcatctggc ctagcctctt cttttaattg gattggttct ttcctagttg gcttgctttt
     9361 tcctgtgatg actgcttcta tgtctcaaga aatcgttttc ggcatttttg ggatcatctg
     9421 ctttttaggg gttttattta ttcaagagat cgtaccagaa actcgtggga aatctttaga
     9481 ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc
     9541 ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaacc gcttagtttc
     9601 cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt
     9661 gaccagttgg taggagaaca ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg
     9721 agcgatgaca aaagctctca caaacattgc gacgagtaac cgtaggagca ataagaggcg
     9781 gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt tcccgttcaa ctagtacagg
     9841 ttcatagaga atatttggct gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc
     9901 ggcatcttgt cccaactttt cttttggatg cgtcaatgtt gtcaattttt tattccctaa
     9961 atggcttaaa gaagaatcgt ctgttcctac gatcgatagg tcatctggaa tatgatagcc
    10021 attttctgtc aacttatcta gcattaaact agctatttga tcgttatagc aaatgatgcc
    10081 atctaaagct tgttcttctt tcaaccgttg tactgttttt tcagcgattg tttctcgtgt
    10141 ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc acattatact gttcacacgc
    10201 gctgataaaa cctttcatcc tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa
    10261 cagacgctga tgtccttttt ttatcaaata ttctgctgcc ataaaaccga ctttcacatc
    10321 atctaaacaa atataagagg catttaactc ttcataagca gcattgatca tcaccatcgg
    10381 aatgccttgt tctctcaaag caacataaag tgctaagttt gggttatact gattactttt
    10441 cgtaggctca acgatcaaac catctacccc ataagcaatc attttcttca aacactcttt
    10501 ctccaattcg tgatcgttat tcgtactggc taaaagcaat gaatacccag attctcgtaa
    10561 ttcattttcg attcctcgga taatcgacgg aaaaatataa tcagagagat atgtggtgat
    10621 gacaccgatc gtttttgttc tgttcaatgg tgttttttct ttcttatagc tgtcatctac
    10681 atatgtgccc gacccttttt ctttacgcag atagccctca tttaccaaca cagcgattgc
    10741 ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc agcttgaatt ctgagggaat
    10801 ggttgtattt acttcgtatt cctcacgaag gatcttttgt tttatctctt ctgcaatatc
    10861 ttgatatttt gccatttgcc ttgccccttt ttatttgtac gtacatatat cataacgagt
    10921 ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc tcataaggca tacggaacag
    10981 gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc
    11041 gattaggatt gtctaacgga tccccttttt ggttcttctc tggtcgagca tgatagatca
    11101 aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt gtgaccagga ccaaattgct
    11161 tatttttttc agaagaaacg aatactggct ccgataattt atgccatgat tcttttaata
    11221 aaagattctt gtgctcatca gcccacagca ggcccatggc atagttttca tctgttgcac
    11281 taccagaata cgtaatgaat attttcccat tgcgagtaag gactgctgga ccttcattta
    11341 ctgaaaaacc ccgtttttcc cattcatact caggtataga aaggaggatc tgcttcccct
    11401 ttaacgtcca aggattatcc atttcggata gataaagatt agaattccca gtaatttcag
    11461 gatcttgttg ggcccacaca taatatagct ttccttgaga agagaaagtt gttccgtcta
    11521 aactgaaagt ttcccttttc gtttttattt gtcccttttc tatccaatct gtattcattg
    11581 gatctatatg ctgattttca agtacgaaca tgcggtgatg atgagattca tcacgtatag
    11641 ttttatgatt gcttgcagca aagtaaatat accattttcc attgatataa tggacttcag
    11701 gtgcccagat caattcactc atcggtcctt cttcatgagc ctgccataca atcaggcttg
    11761 cagcatgttc taattcattc agtgactttg cccttcttaa ttcaatcact tggtagcctg
    11821 gaaccgatcc tgtaaaataa taataaccat ctgtatgttt atatacccaa ggatctgccc
    11881 gttgccgaac gataggatta ttataaagtg tcttcatcat aacaaatttc ctcacatttt
    11941 ctttattcta attacattcc aagagtattt ttctatccct acatttaatt cgtcatgttt
    12001 ttctattgaa aaatagttta tttcaggttt tattttttct ggttcttcac gtgtattttt
    12061 gtccaacaga tcgctactgg ttaatgtgta gtgctctcct tgatctagca aattgaaatc
    12121 atgttcaatc gttatctttt gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg
    12181 attgccgtta tctacgatca cactgtccac ataaggaaca ttttttatct tttcacaatc
    12241 ataaacaggg caaatgattt gttgtttcaa tacttgacct ttagcatatt tcgataagtg
    12301 ttgcatcaca taataagttg gattcctcca tgcttgtcct ttagcatctg tcagtattaa
    12361 aggtattgtg tttactaaca atgattgaca actaatcttt acgcgatctg cgtgtcgtaa
    12421 aatagccagc cctaatgaac caaataataa tgtatcttcc atagtaaagt aacaccaatc
    12481 gatgggacta ccgacttcaa aatccctatg tcttttttcc ggttgggcat gtacattcca
    12541 ttcatcaaac gctagataca ttgttttctc acttctcaaa tatcctttta cataatcaca
    12601 agtagcaacg atttcttcaa tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc
    12661 tgtatcatca cgctcatttt taggaggttt agtcaagtct tcttcatcat acttatcaat
    12721 atagttatgc aaagctaaat aatctacatt ctcataagct tcttctaatg cgatacgatc
    12781 ccattcaggg tacgatggta aacgtggcgt agagcttcca actaagattg tttcaattga
    12841 atcatccact aacttcatga cttttgaggc ttcatttgct aacctaccat attcataagc
    12901 actcttagcc cccacttgcc atggaccgtc tagctcattc cctaaacacc ataatttaac
    12961 gccaaaaggc tttttttgcc cattttttat tcttaaatca ctccagaagg ttccttttgg
    13021 aaaattacaa tattctacta aattttttgc ttcatcaatt ccttttgttc ctaaattcac
    13081 cgtgaatatc ggtttcgctt gtacatgact gatccattta aaaaattcgt ggagtccaaa
    13141 agaatttggt tctatacctc gccatgctaa atcaattttt tttggtcgtt gttctttcct
    13201 acctacagta tccacccaat cataaccaga tgtaaagttt cctccaggat agcgaatgac
    13261 acctaattct aattccttta cataagagag gacatcttgt ctaaacccgt caccatcagc
    13321 tgtttcatgg cccggttcat aaatccctgt atagacgaca cttcccatat gctcaacaaa
    13381 tgagccaaac agccgagaac tgatctctcc taccgtaaaa tcctctctga tttttacttt
    13441 cacttcttcg tccaacagct ttccctacct ttcatttcct atagtcaaga taatagatac
    13501 aagtttccat acgccaccag caaagataaa aataaaagga gggaaaagaa atatgagcag
    13561 tacaaatcct aataacaata cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt
    13621 ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag aaatcacata
    13681 tatccaggat aaaagaaaca tggaataaat aagtaaaact acatatccac taattgaaag
    13741 tacgattgtc tgttcttcta ataattgatt aacaagaaaa taaataatga tcgttaacag
    13801 actaaggaac aagttcttac caaaatgttg ctttattcct tgaaagaaaa aataaatgat
    13861 atttcctgat ccctcacctt tccattgcgc cattacataa aaaacagcac atgtagcagg
    13921 cacgatagta ataacaggca aacacacgat tacccagaga atacctaata agatgtaatc
    13981 agaaaccaca atcaactgtc cataaaattt agtctcaact attttcatct tcctcactct
    14041 ttcattccag acatagctgt tccttgaaca aaatatttct gtgcaaaaat ataaaccact
    14101 agtaatggca gcattgaaat aaccgttcca gccattacag gtccataaaa agaaatgttc
    14161 tgccctgtaa ataaagctaa ccctgctggt aaagttctca tttttgaact acttgtcaat
    14221 atcaatggat aaagcaagtc attccaacta ttcatcagcg tgaaaatccc taatgataaa
    14281 agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc acttaatcca
    14341 tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga acgcatcatg
    14401 aagataccaa atgcagttgt catacgagga atgatcaaac ctgcataagt atcaagcatg
    14461 ccaagcaaat tgacttcaat aaatagagga atcatgaaga cttggaatgg gatcatcatt
    14521 gtcagcaaaa cgaaatagaa caaaactgat tttcctttga aacgcatacg agcaaatgca
    14581 taccccgcaa gagaatcaaa gaatacagaa gtgatcactg tccctccagc aaaaatcact
    14641 gtatttttga tgtaatctaa taaagggatc gttgtccaaa ccttaatata gttatctagg
    14701 gtatagctat ctgcaaatat tctaggcgga aatgagataa tgtctttttc gtgtttaaaa
    14761 gaagaaataa tcagccacaa aaaaggaaac gcaataacca acaatataac tagcatacta
    14821 atcgttcgaa tgattttttt accactaact gcttttcgac tattcacagt aactgtaatc
    14881 gagctttctg gtgccataca ggtttccctc ctatttcatt tgtttttctt tccgtgacat
    14941 gtacacatta gtggagatag ctatagttgc aacaatgata aacaaaaccg aagacagtgc
    15001 tgatgcatag cccaattcat aaggtgctgt aaatcctcga ctgtaaatat attgaacagc
    15061 tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca tatacttggt caaacacttg
    15121 catcgaacca atcagattcg ttaaaataca aaaacctaag gagggaacga tctgaggtag
    15181 cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc atttgagcag attcatacaa
    15241 agagggagag attccctgaa tagcagtcaa gagaatcgtc atagtaacac caaaattctt
    15301 ccaaacagtc atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg
    15361 gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc cctacattcg gatcaagcaa
    15421 catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga
    15481 acgacaaaaa cgattgaatc tggtattttt agataatgct actgctgcta ataaaccaac
    15541 gataatttgt gttggcgttt cgagtacggt gaaatataaa gaatgaatca acgaattaac
    15601 tgctcgttca tcactaaata tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt
    15661 aaagaaaata ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg gtatgacagt
    15721 aaataataat aaaactaaaa aagatggagt taaaaacaaa tacgcagcac gatcaggacg
    15781 attccaatat ttggtgaatc cgttcatata gtccacctca tctttcattt tgataaaata
    15841 tacaatcttg gtttaaacga atctgaataa gatatccgaa caatcgctgt gatttttgca
    15901 ataacaccta tctgcaaata ctaggtcgat cattcagata tctgtatctt gccaacagtc
    15961 tatcctgtat aaagttctgt actatttttg cagtaattga ttgatggcat catctgcatc
    16021 gttcatgagt gtctcaggat cttctcctgc taataatttt tcaatcagtg gattgatgat
    16081 atcgttattg atcgcagaga tttgggtaaa acctggtaaa tatggctctg catagtccat
    16141 ttgtttcgat aattcagata caataggatc attttttact tcttcatctt ttgctactga
    16201 atccaagtag gcaggaaaac cattttcgat actccatttt ttaccaattt cagttgtatt
    16261 ccaatatttc aaaaactcat agacagcttc ttttttatta ttatctgtac ttgccggaat
    16321 ggcatatcct acgccatcta aaatagctgt ttttttgtct gaatcactcc cttgtggtaa
    16381 ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg ttatttaacc atggcccatt
    16441 gatttcaatg gctagttgac cagcattcat taaattgtcc atttcggcgc cagaaatcga
    16501 ttctggacca gtcttgtcta catggatcat tttttggatc gcttttaacg ttttaaggtt
    16561 ctcaggagac gcaaattcag acttagtata atcatcgttc actaatttac cgccatttgc
    16621 taacatccag ttatataacg gtgcagttcc atccttattg aagacaaaac ctgatacatt
    16681 tttactacta tcagttaatt ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg
    16741 ttgttcagta tctaatccag cttgtttaaa caaatcatta ttccaataca tatacatgcc
    16801 ttgtacctgc atggggacaa aatactgttt accttctact tcacctaatt caatagccgt
    16861 ttctttaaaa tcagatttat ctactccatc atattcccag aaatcgtcta atggttgtac
    16921 cgctccgttt tctacatact gtgcaaaatc tggataattt aatgcaataa agtcaggtgc
    16981 ttttttagaa gaaatcgctg taggaagttt ttcattcaaa ttggcccaag tcatgatatc
    17041 catttcaatt ttgatgtttt tatcatttgt tttattaaat tcattaacga tttcttgcaa
    17101 tatttcacca tcagatgcag taaacccatt ccaaaaagtc aaagtcactt ctttatcaga
    17161 atcagattct gctgaacttc cgccaccgca agctcctaaa atacctgttg cagctaccaa
    17221 caaagagcca attaaccatt ttttaccttt cattttttgt tcctccttca tctgataaac
    17281 gacatctgac cacattccaa gatagcggtt tcaataaaca attgattttc ccatcttcag
    17341 cttttacatt tgttgaatga cttggtttta cttgttcact tttaggaaga ttagttgctt
    17401 ttgtatcaaa tccactcatt tcagaaaaat caatcactcc ctctaatgat aattcagaca
    17461 agtgaacagt tagattcatt tcttcttctc cacggttaac tgcaaatatg acaagttctt
    17521 tgtcatttat tacggctata ctttctaaat acggcacttc ttcaaaatct tccgtcgcat
    17581 agtttgctga acgaatttgt gggcttaaca cctctccttg tccatacaca gcagcttgca
    17641 taaatggata atagatcgtc tgtctccaag cttctccgtt tgtttctgtc ctgattggtg
    17701 caataacgtt taccaattga gcaagacaag caatctttac tctatctgca tgtttcaata
    17761 aagtgattaa tagacagcct aatagtaatg catcttcaaa attatagtta tcttctagta
    17821 aaggaggagc aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa
    17881 tattccattc atcaaaggac aggttgatct gcttcttact atgtttcttc gccttgactg
    17941 tatcgcacat cgctgtcact tcagaaatga accgatccat gtctaaggaa cgtgcaagat
    18001 aatgagaaag atcatttttt tggttgccat aatattgatg cagagacaaa taatctattt
    18061 gatcgtacgc ttgatcaaga actgttaact cccaatcacc aaatgtaggc atttgccgat
    18121 tcgaacttcc gcataaaacc acctcaattg aatcatctac gagtttcatc gccttagccg
    18181 tttcctctgc taaccgtcca tattcttccg ctgttttatg tccaatctgc caaggaccgt
    18241 ccatttcatt ccctaaacac catgtcttaa ttccaaaagg gctctcttga ccattcgaac
    18301 gccttaaatc actccaataa gttcctttag gaaaattaca atattcgact aaattacgag
    18361 ctgcatctac tccacgagta cccagattga cagccatatt gatttcggcc cccacatgct
    18421 gacaccagtt ataaaattca tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta
    18481 aatccaacct tctaggtcta tttgctatgg gcccaatact gtcttcccaa cgaaatcctg
    18541 acacaaaatt tccaccagga tatctaatca tgggaatgtt caattctttt accaagtgaa
    18601 tgacgtcttg gcgaaatcca cttttattag ctgatggatg atctggctga taaatccctt
    18661 catatactgc tcgtcccata tgttcaataa atgaactgta caaccgcgga tcaactttgc
    18721 ctacacgctt gacactacta atcaacattg tggcttccat aactgacctc ctttaattta
    18781 tacatgaata atacacctcg ttgaaagcgt tgtcaataat ataaatgtta aacttactta
    18841 ttttttatcg attttaatta accaaaatat taataatgat taaaacaaat aatttaatat
    18901 aacttttatt aacaaatatg ttaatatata agaaaaacct tttaagaggt gatatatatg
    18961 caactacaaa taaacgaaga atccttaccg gtatatgaag cattagctag taaaacaagg
    19021 atcaagatca tccagctact ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa
    19081 cttggagtga gcagtgctat cacgacgatg cacgtgaaaa agctagagga agcaaacatc
    19141 atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat
    19201 attagttttc cagaaaaaat attcaatgct tttgatacga aagaaacatc gatacctatt
    19261 ggtcactata caaattatgc aattgaacca acatgcggtt tagctacgat ccatgatttt
    19321 atcgggaaag tggatgaacc acgctatttt atggatcctc gaaggatgga tgcccgcatt
    19381 ctctggttta ccagcggatt tgtcgagtat caggcaccta attttttaaa tacagaggat
    19441 acattggaga tgttggaagt ttctgtggaa atttcttctg aatttccttt ttcaaatgac
    19501 aattggcctt cagatatcac tttcagccta aatggtgtgg agctaggaac atggacgagc
    19561 cctggagatt ttgctgatat ccgcggtaaa tatacgcctg attggtatcc agataatctt
    19621 aatcaatacg gcttgctgaa gacgatccga attaccaaac acttgacgaa tatgaatgga
    19681 gaacccttgt caaacatcac gatcaacgac atccctaaag agcaagacac ttggcatttg
    19741 cgaatcgaag tgaaagacga tgccaaacat gtggggggct gtactttatt tggtaaagga
    19801 ttcggtaatt acgatcagga tatcaaatta aaagtttact acagctgata tttttctttt
    19861 ctattttata aaacaaaaac agccaatccc tatgaaggga cggctgtttt tgctttattg
    19921 ttatttccag aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt
    19981 ccaccatgct tcgatttttt cttgtgcttg agcgggaact tcttttcctt ctagataatc
    20041 atcaatttca ttatatgtga cacctagtgc gacttcatct gcaatcattg gtttcccatc
    20101 ttcaagatct gcagtcggaa tttttgtgta taatttttcc ggtgcattca atgcttgaag
    20161 caattgtttc ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc
    20221 accaaatttc gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc
    20281 tcctgctctt tctccagcta cggcatattg cgtaatcata cgttgacgtg ctttgatatt
    20341 tcctttgtta aaatcgctga ttcccacacc tgcttcgctt aatacttgta cttgtgcatc
    20401 aacagctggt ttgatattta ctcgcagact aacatcgggc tggataaagt caagagcagc
    20461 ttttgcatct tcttcatctg cttgttcacc ataaggaaga cgaacagcga taaattgata
    20521 gttttcatct tttgtttctt cacgcatttc ttccatcgtt agttgcgcca aacggcctgc
    20581 taaagtagaa tcttggccgc cgctgatacc tagtacaaaa gttttaagaa acggatattt
    20641 tttcaaatat gctttcataa agtcaatact tttacgtatt tcttcttttg gatcaattgt
    20701 tggtttgaca cctaattcat tgataatttc tgtctgcagg ctcatttatt ctcctcctat
    20761 gcttctgtat gaagttctgc tacgcttttt ttcattcgtt ccatgatagc cattttatgg
    20821 ttccagcatt cagtagacag atcgactggg tatttttgcg gattcaagtc tcgtttatac
    20881 tcttcccata gtgaatctag attttctttt gtatattctt tgatttcgtt tagtgcaggt
    20941 aattcgtaaa ctctttttcc ttcgataaag atatcttgta aaactgggcg agctgtaaag
    21001 tctcgaacag ttttattgat aaacgtatgc actggatgga acatgaagat agcttcttct
    21061 tctcgagggt cttcatccca taatgtcacg tagtctcctt cagatttccc atcaaaattt
    21121 cgagtgatgc gccacacttg ttttttccct ggagtagtta ctttttctgc attgcttgat
    21181 aacttgatcg tatccatcat ttttccttca tcatcttcga tcgatactag cttgaatact
    21241 gcaccaaggg ctggctgatc ataggctgtg atcaattttg tcccaacacc ccatacatca
    21301 attttagctt tttgcatctt taaattcaaa atcgttgctt catccaagtc attcgaagca
    21361 tagatttttg cttccgtaaa tccggctgca tctaattgtt cccgaacacg cttagatata
    21421 taggccatat ctccactgtc gatgcgtacg ccttggaagt tgattttgtc acctaattct
    21481 ttcgccacgc gaatcgcact tggcacacct gatttcaacg tatcataagt gtcgactaag
    21541 aacacgcagt ctttatgggt tttagcgtag gccataaatg cttcataatc atttccataa
    21601 gattggacta acgaatgagc atgggtcccg ctagctggta tcccaaatat cttcccagca
    21661 cgtacattgc ttgttgcatc tgctccgccg atataggccg ctctagttcc ccagactgct
    21721 gcatccagtt cttgtgcccg tctagttcca aattccaata gtggatcttc tccgatgaca
    21781 gatttgatac gagctgcttt tgtcgcgatc aacgtttgga aattgaccat attcaacagt
    21841 gctgtttcta ctaattggca gtgcgctaaa ggcccttcca cttggatcaa tggttcattt
    21901 gcaaaaacta gttctccttc acgagcagaa cgaactgttg ctttaaattc aaaattttgt
    21961 aaatactcca aaaaaccttc aggatagact tctaatccac gcaagtaagc aatatcggaa
    22021 tcattgaacg tcagattctc taaataattg actaaccgtt ccaatcctgc aaaaaccgcg
    22081 tatccgtggt taaaaggcat ttctcgaaag taacattcaa aaacagcatg taaatcagct
    22141 cgacctaatt cccagtatgt ctgcatcata ttgatttgat atagatccgt atgcagcgtc
    22201 aaactgtcgt ctgggtaagt attattcatc ttgtattact cctcttggca agttttcatt
    22261 tcctacccat tataacaaaa aaaattaata ggaaaagaaa tcttccattt tttctgaaaa
    22321 agcacatgtt ttacggatat ttcttgctaa aaatgaactc catctgctaa aactatcctt
    22381 tcttattggt atttaaccta aacgagaaag aatacttatt ttattcaatg tatccgagac
    22441 acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat tttcctacta
    22501 caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc tctacaacat
    22561 aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct tcttatcccc
    22621 aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa ttatgacaag
    22681 atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa aaagatacaa
    22741 agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca atcagtctcg
    22801 ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat gattattagt
    22861 cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc tttatttgga
    22921 cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg gactttgaca
    22981 tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg ccttgtgatt
    23041 tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac caatagcagt
    23101 tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc aaaaaagaat
    23161 tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg tgtttcgatt
    23221 agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta agttatgtta
    23281 ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca aaaggattta
    23341 ccttttatca cttaatagta taacataatt tcatgagaaa aaaatacaat tttttcttga
    23401 ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata atggagtaca
    23461 caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc ttttatgatg
    23521 agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt tatggcaaaa
    23581 aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc aagttggatg
    23641 atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta atcaagcatc
    23701 agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact gtccaacaga
    23761 cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta
    23821 aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga caaaaatacg
    23881 gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa gaaacgtatg
    23941 aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac ttaaaagaac
    24001 cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg ttatcttaca
    24061 gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac gaagccgatg
    24121 aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg
    24181 aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta cacgtaaaat
    24241 gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt ttggtgtctg
    24301 ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt gaataaacgt
    24361 ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg ccatttctcc
    24421 gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga tcagcaaatt
    24481 atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc
    24541 aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa agaagcctgc
    24601 ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat caaaagatgg
    24661 aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc tgccacttcg
    24721 ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa gaccttctgt
    24781 aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga tttcttcaca
    24841 aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat tggcacgaga
    24901 cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc ttcgctcttt
    24961 ttctttctga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc
    25021 aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca gaatcactcc
    25081 aatgcttttc gtttttccat aacttaactg tctggcattt gcattaggga tataatcgac
    25141 ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg gatgtccatt
    25201 gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat ctcgaatgtt
    25261 tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc taccacagct
    25321 ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat ccgctgcccg
    25381 ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc ttttactata
    25441 ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa ctcgacaaac
    25501 gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt cattgcaagc
    25561 cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt
    25621 tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt ttaaatt
//
LOCUS       DAGCXG010000029        25568 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGCXG010000029 DAGCXG010000000
VERSION     DAGCXG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.030"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            165..2225
                     /locus_tag="MB022_001244"
     CDS             165..2225
                     /locus_tag="MB022_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBT4466253.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2308..2877
                     /locus_tag="MB022_001245"
     CDS             2308..2877
                     /locus_tag="MB022_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466254.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3052..3858)
                     /locus_tag="MB022_001246"
     CDS             complement(3052..3858)
                     /locus_tag="MB022_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466255.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3996..5510
                     /locus_tag="MB022_001247"
     CDS             3996..5510
                     /locus_tag="MB022_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBT4466256.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5913..6896)
                     /locus_tag="MB022_001248"
     CDS             complement(5913..6896)
                     /locus_tag="MB022_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBT4466257.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6901..7548)
                     /locus_tag="MB022_001249"
     CDS             complement(6901..7548)
                     /locus_tag="MB022_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4466258.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7633..8265)
                     /locus_tag="MB022_001250"
     CDS             complement(7633..8265)
                     /locus_tag="MB022_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4466259.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8281..8832)
                     /gene="coaC"
                     /locus_tag="MB022_001251"
     CDS             complement(8281..8832)
                     /gene="coaC"
                     /locus_tag="MB022_001251"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBT4466260.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8829..9566)
                     /locus_tag="MB022_001252"
     CDS             complement(8829..9566)
                     /locus_tag="MB022_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBT4466261.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9563..10552)
                     /locus_tag="MB022_001253"
     CDS             complement(9563..10552)
                     /locus_tag="MB022_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBT4466262.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10810..11352
                     /locus_tag="MB022_001254"
     CDS             10810..11352
                     /locus_tag="MB022_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466263.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11369..12454
                     /locus_tag="MB022_001255"
     CDS             11369..12454
                     /locus_tag="MB022_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466264.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12466..13272
                     /locus_tag="MB022_001256"
     CDS             12466..13272
                     /locus_tag="MB022_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466265.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13273..14100
                     /locus_tag="MB022_001257"
     CDS             13273..14100
                     /locus_tag="MB022_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466266.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14097..15170
                     /locus_tag="MB022_001258"
     CDS             14097..15170
                     /locus_tag="MB022_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4466267.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15598..18294
                     /locus_tag="MB022_001259"
     CDS             15598..18294
                     /locus_tag="MB022_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4466268.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18371..18859
                     /locus_tag="MB022_001260"
     CDS             18371..18859
                     /locus_tag="MB022_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4466269.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18871..19365
                     /locus_tag="MB022_001261"
     CDS             18871..19365
                     /locus_tag="MB022_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4466270.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19386..20195
                     /locus_tag="MB022_001262"
     CDS             19386..20195
                     /locus_tag="MB022_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4466271.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20192..21013
                     /locus_tag="MB022_001263"
     CDS             20192..21013
                     /locus_tag="MB022_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4466272.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21064..23163
                     /locus_tag="MB022_001264"
     CDS             21064..23163
                     /locus_tag="MB022_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBT4466273.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23160..24278
                     /locus_tag="MB022_001265"
     CDS             23160..24278
                     /locus_tag="MB022_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBT4466274.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24317..25195
                     /locus_tag="MB022_001266"
     CDS             24317..25195
                     /locus_tag="MB022_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4466275.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25386..25538
                     /locus_tag="MB022_001267"
     CDS             25386..25538
                     /locus_tag="MB022_001267"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4466276.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8079 a         4453 c         5412 g         7624 t
ORIGIN      
        1 taaaaagtac ataagttatt attccgattt tcttgaaaca ataaaaaatc aaaaaaaata
       61 ctctcctaat agtccatctt cttttgattt tatttatata atgaaatcat cttatgaaaa
      121 ttactacttc atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac
      181 gttcaaaaca actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa
      241 aacactacgt taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata
      301 tgcatgtacc cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg
      361 agaccactga ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc
      421 tacttcctgc attgacttcc tataataaag tcagttatcc tccaacgata aaccactact
      481 ggttaattcc taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta
      541 tcttttccga tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt
      601 tgaatcatgc tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg
      661 aaaaagatta ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt
      721 cgatatctga tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca
      781 aagtaatttc aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt
      841 ccttgtccta ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg
      901 ttcgtgctga tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt
      961 acgcccctaa agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag
     1021 aagaaatcaa aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat
     1081 taacttatat agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag
     1141 aagcatctgc tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag
     1201 aacttcaagc agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg
     1261 ctgaattttc tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag
     1321 tcacttctat ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg
     1381 agatccttga agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa
     1441 ttacctttac ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg
     1501 ttcatccaaa cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag
     1561 gtaaacccgt accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg
     1621 gttatttgac caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt
     1681 cactccaaga actccatgct cagtcactat tacatgtttt accattaaac aaacagatag
     1741 atcaaaaaga acaaaaaaga caagaagaaa gcttgtcaga aaaaaaacaa caaatggctc
     1801 atgcagtttc tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat
     1861 ctagaaaaat ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag
     1921 tttctggatt ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat
     1981 ttgttgctaa acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa
     2041 aacgcttgtc aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta
     2101 gtccgggaac acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc
     2161 gaggtatggc aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt
     2221 tctgatttat atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc
     2281 ataatggaga cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc
     2341 tggatgacaa gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa
     2401 tttcgatttt atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg
     2461 gaatttcaaa cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct
     2521 gaagaacttg aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc
     2581 tgtaccgctt ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca
     2641 gtgaaaccag attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa
     2701 gaatttccta ccctccatac ggacggacca atctatcttc acacacagac atggagccat
     2761 caggcgattg ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc
     2821 acacctaatc cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga
     2881 aatggtgaaa taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt
     2941 acttttttga gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg
     3001 ggcagtgaca aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt
     3061 gattttttta ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata
     3121 gaggtaacca aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat
     3181 agtatccatg attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc
     3241 atctgcattt tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt
     3301 tccgtcttta cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc
     3361 gaattgcagt aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat
     3421 cgaaaaacag aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt
     3481 tttattgagc aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc
     3541 atgtagaaaa gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac
     3601 tgttgaacta aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt
     3661 taatatcttt tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat
     3721 tgtatttccc cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg
     3781 taataaaata tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa
     3841 ggttttcaaa tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc
     3901 gtgtgtttac caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc
     3961 acttaatata gtataaggag aaaaggggga tacaaatgaa aaaattagtt gctggtttat
     4021 taacagccgt tgcacttctt gctatgagcg catgcggcgc agatcagaaa aatacagcgc
     4081 agacaagttc tagtacgaag gaatctgccg aagtatcatc aggcgcttct gaacaagtat
     4141 atacagatcc aaaagaaatg aaagaatctt atgatgtcgt gattattgga gctggtggtg
     4201 cagggatggc agcagctatc gaagcaaaag atgctgggtt gaatccagta attttggaaa
     4261 agatgcctgt tgccggagga aacacatcta aatcatcaag cggtatgaat gcttcagaaa
     4321 caaaatttca aaaagaagaa ggaatcaaag actcgaatga tgccttctat gaagaaacgc
     4381 taaaaggtgg tggaggaacg aatgataaag aaatgcttcg atactttgtc gatcactctg
     4441 ctgatgcggt cgattggcta gatcaaaacg gtatcaaact ggacaactta acgatcactg
     4501 gagggatgag tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt ggtggatact
     4561 tagtggaggg gttattgaat aacgtccatg agcgggaaat ccctattttt gtcaacgcag
     4621 atgtgacaga cattaagaaa aatggggaaa cggtcaatga agtgactgta catgttcaag
     4681 gagaagaacc aaaagaggtc accggaaaag caatcgtagt taccacaggc ggttttggtg
     4741 ccagcaaaga atttattgaa aaatatcgtc ccgatttagc tgattatatt acgacaaatc
     4801 aagaaggatc aactggagat ggtatcacca tgattgaaaa attaggtgga caaaccgttg
     4861 atatggataa aatacaaatt catccaactg tgcagcagga tgagggggtc ttgattggtg
     4921 aggttgttcg cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg
     4981 aaatggatac aagggataaa gtatctgcag caattacagc attgcctgaa aaatcagctt
     5041 atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa
     5101 aaggatatgt cacaactgga aagacaatgg aagaattagc agaaaaaatc gatttaccaa
     5161 aagaaacact agcaaaaaca gttgatggat ggaatacgga tgttgcggag aaaaaagatg
     5221 ctcaattcaa ccgtgccaca gccatggaac atgacttatc gtctccgaat tattatgcga
     5281 tcaaaatcgc accaggaatc cattacacga tgggcggtgt gaaaatcaat acgaacactg
     5341 aagtattaga taaagaaaac cagccaatta gggggttata tgctgctggt gaagtcacag
     5401 gtggcttaca tggaaacaac cggatcggtg gaaattcagt tgcggagatc attatttttg
     5461 ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga aaatgaatga aatagaacgt
     5521 aaataaaaag aggttgtgaa atcagcgttt tgagctaata ccgagggtca gctgattgaa
     5581 cactgtttag tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     5641 gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt
     5701 tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     5761 gctctaaaca cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt
     5821 cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt
     5881 ctgtcccagc ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt
     5941 ccgatgtttt tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat
     6001 gtacggtcaa tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata
     6061 aaagtttgtg cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc
     6121 agttggacac gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct
     6181 agcataccaa tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag
     6241 taagaagcgc cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt
     6301 acctcttctt gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta
     6361 caaaaggcga gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt
     6421 ctagctaatt ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt
     6481 tcgccttctt gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct
     6541 gagattccag cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca
     6601 ggcatcacag catattcggc ataaccacct agattgacta atccgtatac ttttgtccca
     6661 ggagtaaaag tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc
     6721 aaaatagggt agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg
     6781 acagctgctg tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact
     6841 tcaatttgtt ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat
     6901 ctattcctcc tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag
     6961 tactagagtc gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac
     7021 tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt
     7081 aacttcttca tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg
     7141 tttcagccat tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca
     7201 ttttgctggt tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt
     7261 ttcagctgcg atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc
     7321 ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc
     7381 cacggaccaa gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg
     7441 ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat
     7501 caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct
     7561 ttcgacgtca aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg
     7621 ctacgatgac tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca
     7681 tactgctgca gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac
     7741 cgctagaata gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg
     7801 ctggaataaa aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag
     7861 tccagctaag aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt
     7921 ataaacgaaa taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc
     7981 tttccagctt ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa
     8041 aggtgtttgg ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa
     8101 agcacctagt cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt
     8161 tgcattgatt ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat
     8221 agctaaaaac atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt
     8281 ctaagcattt ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc
     8341 taaggcacct tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg
     8401 atagccgaca gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt
     8461 cgtgttcatg gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt
     8521 tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg
     8581 cgcgatgaga aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt
     8641 tgattcttgc atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg
     8701 cgtgataaaa tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac
     8761 gaattgactt gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa
     8821 gatctttttc attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca
     8881 gatttacttt gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc
     8941 tcgccatgga tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga
     9001 ctattttttg cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca
     9061 atgatttttg cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc
     9121 aaaacgagca caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt
     9181 tcattttccg caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt
     9241 gccagctgtt cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg
     9301 ataatcgcat cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt
     9361 gcagattcga tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc
     9421 gtagtcacgt aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc
     9481 cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt
     9541 cctccagctg tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt
     9601 ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt
     9661 ggacgaccag ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt
     9721 gatctattgg tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag
     9781 gcaagacagt cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt
     9841 tataagagat caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat
     9901 agtcgatttg ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta
     9961 cgacttgcaa agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg
    10021 caatgattga ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc
    10081 cagaagcacc agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt
    10141 tcacacacaa catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca
    10201 tattttttgg tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc
    10261 ctggatgggt gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac
    10321 gtggattgtt actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg
    10381 ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat
    10441 aggcaatttt taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg
    10501 ctgtatggaa aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga
    10561 cactcctttt aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt
    10621 ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc
    10681 aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat
    10741 ttcgtatact aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg
    10801 gaggaaatta tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca
    10861 caagaagaac taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga
    10921 gatttaagtt caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact
    10981 ccagaagaat tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat
    11041 catacggttt atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg
    11101 aatgaaaaag aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg
    11161 tttgaacctt cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc
    11221 ttaggagaga cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa
    11281 caacatcaat taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa
    11341 tcatatttgt aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt
    11401 agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg
    11461 aaaattttat acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat
    11521 agctggattc accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga
    11581 tttgccagct aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca
    11641 tatgaatgtt tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga
    11701 tatcgaaaag aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg
    11761 ggagattagt gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt
    11821 aaatgaaccg aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg
    11881 tacagatatg cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt
    11941 tgttactcat gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa
    12001 agggaaaatc gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt
    12061 cgttgctgat tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct
    12121 agttgagttt gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc
    12181 agttgaagtt gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact
    12241 agtcgtaacg gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga
    12301 tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt
    12361 aggattggcc tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga
    12421 attcgatgca cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc
    12481 aggatctatt ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta
    12541 atgatcattt atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta
    12601 tcttatttta cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt
    12661 ttgatcacgt ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg
    12721 aagcacaagc aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt
    12781 aaagcttacg catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat
    12841 tttatcggga tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca
    12901 acgtatatcg agattccatt tatgattatg ccaattttca atgcattgga agagatgaat
    12961 ccttctttga tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga
    13021 gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct
    13081 tctctttctt tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga
    13141 acggctatcg aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt
    13201 gtgattttaa tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga
    13261 ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta
    13321 ttcttatatg caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg
    13381 aacagcttta caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg
    13441 atcattatcg tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc
    13501 atcgggacat ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg
    13561 ttgttgagca tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc
    13621 ttgattttct ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat
    13681 attgctttca gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac
    13741 gattcaatgg tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat
    13801 atcattttgc cttatctttc tccaggaatc atagctggat attttatggc atttacctat
    13861 tctcttgatg attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca
    13921 gtagagatct attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg
    13981 ttagttttct tgttctccat gatattggtc attggatact actttattag taaagaaaac
    14041 cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga
    14101 aaaaattaca atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg
    14161 ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact
    14221 ggggagatta tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag
    14281 tgaattatga aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga
    14341 caaactacga catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac
    14401 tagtacttcc attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt
    14461 tagacttgga ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag
    14521 ggatcgtata taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt
    14581 ggaagccgga attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc
    14641 tttctttgaa tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag
    14701 cagcaaataa attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca
    14761 aaatgtatat gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg
    14821 atatgatgtg ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt
    14881 ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca
    14941 tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta
    15001 caccaaatga agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct
    15061 atccatctga tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc
    15121 taggcatcta taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact
    15181 cagtaagaat ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg
    15241 agttttccaa tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt
    15301 tttctaccac gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga
    15361 gacgggctag taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat
    15421 gcaaatatac ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa
    15481 tatttgtagc tatgaaaaaa cataaaaatc atattagata aaaactaaat tggtagataa
    15541 attattttgt cagatatctg aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg
    15601 gaatacaaat atagaataga catattgcag ccgatagttt attatgcttt tagacacatt
    15661 aaactggagg aaaaaatgaa aaagagcgct aaagatcgag tttttgatga gctgatacga
    15721 ctaacaggga atatgtcttc tgtcactgcc gagaaaattt cgaagaatct aggtatctct
    15781 cgtcagaatg ccagccatta tttgacgaga ttagtggaag ataaaaaggt cgagaaatta
    15841 ccgggaaagc ctgttttgtg gaagccttta gatgaatatg cggttgtaga taatacggag
    15901 caaataaacg aagcctttca ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt
    15961 caaaaatgta tagccgctgt caaatatccg cctaacggac tcagtgtatt aataaatgga
    16021 gccacgggtg ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc tatccatgag
    16081 caaattattg aaaaagatgc accatttgct atcttaaact gtgccgatta tgcggacaat
    16141 cccgaactgc tttcggcaac actgtttgga tacaagaagg gggcctttac gggggctgaa
    16201 aaggataccg atggtttatt ggcaacggcc aacaacggct atctttttct cgatgaggtc
    16261 catcgcctat cgaaagaaaa tcaagaaaag ctctttctat ttattgatac tggaaactat
    16321 cgtccagtcg gtgaaaatgt aaattggcat tctgctaagg ttcgttttat tttcgccacg
    16381 acggaaaaag gtgaaaatta tttattagac acctttgatc ggcgtattca aattagtgtt
    16441 atgttgccaa cttttgaaga tcgccccatt cgagaacgat tagagctgat tcaattattt
    16501 tttcaaaatg aagcggatgt tttacaaaaa gatattattg tttcaagaga agccttgtct
    16561 ttaatgttat ctcatccctt tagtgggaat attggtaagc tgaagaatat cattaaaatc
    16621 agttgtgcgg atgtgtatag tcgaacacaa gaagaaccat taacgattgg gaaaaatgag
    16681 atcttgattc agttaaatat gctggaatcc gaagtagaaa gcttgccgat tgctagttta
    16741 ttgataaaga aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga ttatgattcg
    16801 attttggaaa aaattactca gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg
    16861 ataaagaatg aagtacagca tttggtgcat cacattgata aggattcttt tttatcaatg
    16921 agtagaaaat tgatgattca gatgtttaga aaggtctggg tatccgtttt aggaaaaacg
    16981 tacggattga cgacgagtgg acctgttgca gaaattatta gcagatccta tgtcatttcc
    17041 ccaagctata agacagactt aaccgggata aatcgtacat tagctgcaaa tctacctcgg
    17101 acgacttact tatgtaatca gttcttggag catttaccac cgctctcaga aaaggacgag
    17161 aactttctaa gactgcttat tgtcgtgact ttatctgatt atgtagatga atccatcgag
    17221 ctgaagggcc tgttgcttgc tcacggagat agtacggcta gcagcattca agcagtagtg
    17281 aatcaattat gcggcaatta tgtttttgaa gcgttggata tgccagtaga gagtaatgtg
    17341 tcagagattg tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac agatagtcta
    17401 gtgctgatcg tagatatggg ctctttaaat caaatttaca cacagatcaa gaacgattta
    17461 aaaggggagt tacttcttct gaataatttg acgacttcca ttgcacttga tattggcttg
    17521 aagatgacga tgaatgcgcc atttaaacag attgcggagg atgcagcaga caagtattca
    17581 atcaatgttc aatactttga aggcttttct caaagtagca acattatcat ttcctgtatg
    17641 tcgggtttgg ggatttcgga caaattgaaa gatgtttttc gtcatcattt atcggatgaa
    17701 tccatcgaag ttttcacgaa ggactacaga gaactacgtg aattaatcga ccaaaatgat
    17761 gaaacttatt ttgacaagac aaaattggtc atcacgactt cggatcttcc acgttccttt
    17821 tcaattccta atattaatgt ttatgacatc ttagacggtg atggagcagc caatttggag
    17881 catattttga gtcctgaaat gaagccggca gaatttaacc gcttgattca ggagctagta
    17941 aaattctttt cgttggaagg aattgcggac cgtttgaact ttctgaaccc aacgatcgtg
    18001 atgagtgaag tggaaacagt catttctaaa tatgaaaatt actatcattt cactttaaat
    18061 ggccgtgtaa aactaaatct ctatatgcat acggcattga tgatggaacg tttattcttg
    18121 gctcggacag aaaaatctga agaaccgaat tatgatttga aaccagatga agatgaattt
    18181 tattatgttt ctagaagtat ttttcaatca atggagatga aatataattt taaagtcaat
    18241 gcttatgagc tttcgctgct ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc
    18301 taaaaaagtg tctaatctgt tttttttagg ttagacactt tttttaatgt gaattaacgt
    18361 gattaaacca atgtttttaa aagttggcac aggaattgct tatataaaag tgaacagaaa
    18421 gaaaggaatt aaccatatga agaaaattct aatagcgact cacggtcact tagcttcagg
    18481 ctttcttagc agtattcaat tactgacggg gaaggttcaa gagattacgg tgattaatgc
    18541 ttatgtggat gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc aggtgaacga
    18601 gcaggatcag atttttgttt ttaccgattt atttggcgga agtgtcaatc agaagattac
    18661 caaaaccttt ttggagaaag agattgctgt aactattttg tctggcttta atctgccaat
    18721 tctgctcgaa atagtattgg caaccaagga attaacaaag gaggatattc aggaactggt
    18781 aaataaatgc caacaggaat taaagatgac cattcttgat gaggtacatg tagtaaatga
    18841 agaggaagat tttttttagg aggaagaaaa atgattaaag cagtgcgaat tgatgaacga
    18901 ttaattcatg gacaagtagc gatgacttgg acaaagagtc taaatttaac cgggttagta
    18961 gtagcaagcg atgaggcggc taccaacgat attcaaaaga tgacattgaa gatggctgca
    19021 ccatcgaatg tcaaagtcat tattaaaaca gttgatgacg cgattcattt gttaaaagat
    19081 ccgcgggcga aggacatgcg cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa
    19141 gttgcgaaag ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg
    19201 acaccgccat cggaagataa gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa
    19261 attcaagact taaaagaatt agttgaactt tatccagata ccgtttttca gcctacaccg
    19321 gcgatggaaa aacgttcagc tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa
    19381 aaattatgta tgatgcattt gtagtcgctt tggccgtttt tattggagtt gctggacatg
    19441 aaatgttcgg gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt
    19501 tgatggggga tttgcagacg gggttgattg tcggggcgtc actagaagcc atctttatgg
    19561 gtgtcgtcaa tatcggaatc tctagtacgg cggagccttc tttggctgct gggctggcta
    19621 cagcgttcgc aatcaaaatg ggaggaaacg tggatgcgat tattcccatt gtctttccac
    19681 tagcagtatt aggattacag ttaatgaata tgattttttc ttttgtatgt ggaccgatgg
    19741 ctgctaaatt tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact
    19801 tctttatgtg gtttgtccac tatgcgcttt atgctcttat tccattcttt gccgttttat
    19861 ttggagcaga tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc atgaacggat
    19921 taacagtcgc tggaaatctg ctgccggcag ttgggatggc gatgctgtta cggatgcttt
    19981 ggagtaaaaa tattgctgtg tggtttttct ttggggccgt tgttatggcg tatctcgagt
    20041 taccattgat tgcaattgcc gcagttggaa cgattattgc aattgttata gcccaaagag
    20101 atctacaact aaaaaatata tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa
    20161 cgcttgacca agaagaggag gatttctttc aatgaaatta gctgatggaa tttctaaaga
    20221 tgagaaaaaa ttggttcgga agatgttttg gcgctcgatg actatgtatg cttcggtgaa
    20281 tccggtgacg atgggcggcg gtggtttcgc ctattctatg atgcccttca ttaatcattt
    20341 ctacaaaagt gaagaagatc gagctgcggc gctggaacgt catacacagt atttttcgac
    20401 gaccattcct tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga aggaaaattc
    20461 tgaaaaagct ggttttgacg tggaaagtat caacgcgatc aaaacgagtt tgatgggacc
    20521 attggctgga attggcgatt cattgttctg gggagtttgg cgtgtcgtta ccgcgggact
    20581 agcgatcaac ttagccaatg ccggaaatat tttggcacca attatctttt tagttatgtt
    20641 caatatacct aattacttga tgcgatacta tggcggcgtt ttaggctatt cattaggctc
    20701 taaatatatt gaaaagctgt atgcgaacgg gttaattgaa attctgacaa aagctgctag
    20761 tatcttgggt ctgatcatgg taggtgcaat gacgatcagt atggtcaaat tcaataccgt
    20821 tttgagcttt tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc tcgatagtat
    20881 ttttaaagga attgtaccgt tattggtaac attgagctgt ttcaaattac tagataaaaa
    20941 agtcggcgtc atcaaaatta tttttggaat ggtcgtcttg ggaattttat tgtcattact
    21001 tggaatcgca tagtaggaag ctgcagctac taggctgcag tttttttagc aaggagggaa
    21061 aagatgacta ttaatgaagt aatttattca cccaaacacc cattttttaa tgctgagtta
    21121 aacgacggaa atgtgactac ttcaaacgaa tacagctact atcggaatat tcctgatgta
    21181 aagggattga ccttcgatgt aagtaatcaa aagacattag caaaccttga tgtttgcggg
    21241 acgattcaaa cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt
    21301 tgggtcaata aacagctctc tcaaaccaat tcgttatcct taaaggttga agtaaacggg
    21361 attgtctacc atttgtcaga atcggatcat cgtattgaag tcgatgtgat tcaagactgt
    21421 ttgcctagat atattcatca ttattcgacg tttaaggtca ttgtagtatc ctttgctccc
    21481 atactggcta ataagcgttt aagtatgctt gttcaacagg tttacattgt gaatgaaaca
    21541 cacgaaagcc ttcaagtttc agtgaaagat gtgccttcgt atcaagagaa gtattcggat
    21601 cagcaaaatg tgttgatttc tcaaaaagga aataaggaca cgatcgcaca acaggaagtg
    21661 gcaagcttcg ccattgcttt tgtggaccca aatgcttttg aagaagtaat gacttttcag
    21721 gaatctgata tcgaaaaatg gctgaaggaa acattgaact attttgctca gctatttggg
    21781 cagctgtcaa tgccaaacga tagagttgtc catctctatc gtcgtgcact ttatcaaagc
    21841 ttttctagtt tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg gggcagctat
    21901 cctgctacta atcgaatttg gaataaggat atgtattacg catcattgcc attcaccatg
    21961 tttgaaccgg agttatgtca aaaaacggtt ctatggtttg atcaatatgg cgttaagttt
    22021 cctggttcaa aatttacagg aggtatcaat cattctctga gtaattcgtt atccagtgcg
    22081 ttactggcta gtctttattt tgaacatact ggggatacaa aattctttga gcggtgtccg
    22141 caggttctac tgaatgcttc aagaattatt gaggacatat tagctcaacg tcggtcaggg
    22201 gaaccaatgc tttttccatc tgtttggtta tcagacgcct ttgcacttgg aaagtatcat
    22261 accggcagca atctttgtct ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc
    22321 ctagaacaac tgagtcttag caagcgatac aaaaacattg cctgtaaact aaaggaatca
    22381 atttgtaaac aaatgactat cgatggtcct tttggtgagc aatttttgga gggaatcggt
    22441 gatgaggaga aaacaaccta ttcagtgaag cactatcaaa aaccgattct tgagcaaggg
    22501 ttaatctttt taagtgatgt gattgtggat ggaaaaatta atttattgat gcatgatgga
    22561 gaagaatcag acactacgtt gatgcctttt tatcaatttt tagataataa agatcattta
    22621 tatcagaaca ccatggcctt ttctgctagt tctttcaatc caacctatag cgaggaaatc
    22681 aaaggcatca cttggggatt agaatccggc gccacttttc ctggctttat cactgtttta
    22741 atgagcgagc tgcacaaccc tcaagctttc gtaacccgat tagaagaact aatttgtttg
    22801 gcagatttag acggttcgtg gtggtggtgg ccgtatcgct tagaggctca acaaggagaa
    22861 gtcgtgcgag actttggctg cggcaaatgc ggctgggcat cgggtctttt tgtcagccta
    22921 atgatcagtc aatatttcgg attgaaattc aaaaaaggag tattgcaaat tgatcctgtt
    22981 gataatttaa cgttttcttg gaagaatttg aagttcggtc aagccttcat ttcgattgaa
    23041 tgtacgcaat ccgagttgag tatttcaaat ttgtcagaaa agccattgaa aataagtgat
    23101 acgaaaaaaa ttcttcacgt agaaaaggga aaaacgattc acattcaaag gagaaaaaga
    23161 tgagtatact aaaaaatatt acccaattta aaaaaatcca gagtcgagca atcactcccg
    23221 aaaatccaac aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc
    23281 gcaaaggtcg tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt gcggatatta
    23341 ctggaacggg aataattcgt catatgtgga tgacgattcg tgatcgaaca gaatttggca
    23401 gttttgtttt acgtgattta atcttaagaa tttattggga tgggtcggat agccctgctg
    23461 ttgaaagtcc tttaggggat ttcttttgta atgggtttgg agagcgttgc gaggtgaact
    23521 cgttgccgat agtggttaat cctacagggg gctttaattc ctatttcgaa atgcctttcc
    23581 aaacatccgc taaaattacg atcgagaatt tacatccgaa ggatatgtcc tcattttttt
    23641 atacgataaa ttacgctttg gtggacgaat tggaagaaaa tagcctatat tttcacgcaa
    23701 attggcatcg ggaacgcatt accaaattac aggaggatta taagttaatt gatcgattaa
    23761 agggtcaagg ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa cgttattggt
    23821 ggggcgaagg cgagtttaaa ttctacattg atggggatag tgagtatccg accgtaacct
    23881 caacaggttc agaagattat tttggcgggg cgtgggcctt tcatgagtac gatgagttcg
    23941 gacgtccaca tgcgaaaaac tattcaacag cctttttagg gtatccttac caatcaaata
    24001 gagatgccac aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg
    24061 acgacgcaat gccacgacat ggtctttatc gttggcatct tcaagacccc atctgtttta
    24121 ccgaagattt atcggtcact ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc
    24181 ggcaagacga tgttgctagt gtcgcttact ggtattcaac agaagcttca ggaaatacta
    24241 aacgtcattt cactgatcga gaacgcttgc cgcgttaatg aagtgttttt cacatattcg
    24301 aagggaggaa cctatgatga tatatggggc aattgaagct ggagggacta aatttgtctg
    24361 tgcagtcggt actgaggaat taacgatttt ggaaagagtc agttttccca caacaactcc
    24421 agttgaaaca atgaagcagg tcattgattt ttttaaacct tttaaggaac aattggcagg
    24481 catcggggtc ggctgctttg gtccaattga tattcaatgc aaatcaacga gtttcggtca
    24541 tatcacatca acaccaaaac ttgcttggca aaatttcaat tttgtaggaa cgctaaagca
    24601 atatttcaat attccgattg aatggacaac agatgtcaat gcggcttgct atggtgaata
    24661 tgttgctggt tctggcaaag ggttagcagg tgttgcatac ttcactgtgg gaacaggtat
    24721 cggaggtggt gccttgttca atgggacttt cgtggaaggg ttcagtcatc cggaaatggg
    24781 gcatatgttg atcaagcgac atccaaagga caatttttca ggaaactgtc ccttccacca
    24841 agattgttta gaggggatgg cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg
    24901 ccagaattta ttggctgacg attcgttttg gcagattgaa gccttttacc ttgcccaatg
    24961 tgcatacaat acaacactga tgttttcgcc agatagaatt atttttggcg gtggtgtaat
    25021 gaaacaagaa cacatgaaga aaaaagttca ggataaattt gttgagctta taaatggata
    25081 tgttgaaata ccaccaattg atagctacat tattacgccc gaattaggtg ataatgccgg
    25141 aataatcggc ggattagcat tggccagaaa agcggtaaga aataagcaac cataattaat
    25201 gaatgtttca ttgatactca tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga
    25261 agttggataa agcgaaaatg aattcaaata aaaaggctct aagtcaaatc gtgttgtttt
    25321 cattaaatca ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt
    25381 ataccttgag gatattcact ggtggcgtac acttaaatag taatatattg tgttacttta
    25441 tgtctgtggt aatagttata gttgcttacc aatgtatgtt catttttcga aatgttgact
    25501 tcataatcct tggcttaatg tttgaatttt tttggtgata gattttttca gccgtataaa
    25561 ataaatcg
//
LOCUS       DAGCXG010000030        24752 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGCXG010000030 DAGCXG010000000
VERSION     DAGCXG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.031"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(20..220)
                     /locus_tag="MB022_001268"
     CDS             complement(20..220)
                     /locus_tag="MB022_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4466277.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            537..1724
                     /locus_tag="MB022_001269"
     CDS             537..1724
                     /locus_tag="MB022_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBT4466278.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(1999..3231)
                     /locus_tag="MB022_001270"
     CDS             complement(1999..3231)
                     /locus_tag="MB022_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBT4466279.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3486..4055
                     /locus_tag="MB022_001271"
     CDS             3486..4055
                     /locus_tag="MB022_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4466280.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4233..4673)
                     /locus_tag="MB022_001272"
     CDS             complement(4233..4673)
                     /locus_tag="MB022_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBT4466281.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4831..5595
                     /gene="map"
                     /locus_tag="MB022_001273"
     CDS             4831..5595
                     /gene="map"
                     /locus_tag="MB022_001273"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBT4466282.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5627..6550
                     /locus_tag="MB022_001274"
     CDS             5627..6550
                     /locus_tag="MB022_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBT4466283.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6626..7765
                     /locus_tag="MB022_001275"
     CDS             6626..7765
                     /locus_tag="MB022_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBT4466284.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7758..8558
                     /locus_tag="MB022_001276"
     CDS             7758..8558
                     /locus_tag="MB022_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4466285.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8558..9385
                     /locus_tag="MB022_001277"
     CDS             8558..9385
                     /locus_tag="MB022_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4466286.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9363..10097
                     /locus_tag="MB022_001278"
     CDS             9363..10097
                     /locus_tag="MB022_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4466287.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10197..11063
                     /gene="rfbA"
                     /locus_tag="MB022_001279"
     CDS             10197..11063
                     /gene="rfbA"
                     /locus_tag="MB022_001279"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBT4466288.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11077..11649
                     /gene="rfbC"
                     /locus_tag="MB022_001280"
     CDS             11077..11649
                     /gene="rfbC"
                     /locus_tag="MB022_001280"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBT4466289.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11671..12699
                     /gene="rfbB"
                     /locus_tag="MB022_001281"
     CDS             11671..12699
                     /gene="rfbB"
                     /locus_tag="MB022_001281"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBT4466290.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12797..13648
                     /gene="rfbD"
                     /locus_tag="MB022_001282"
     CDS             12797..13648
                     /gene="rfbD"
                     /locus_tag="MB022_001282"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBT4466291.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13682..15715
                     /locus_tag="MB022_001283"
     CDS             13682..15715
                     /locus_tag="MB022_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466292.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15759..17038
                     /locus_tag="MB022_001284"
                     /pseudo
     CDS             15759..17038
                     /locus_tag="MB022_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            17248..18054
                     /locus_tag="MB022_001285"
     CDS             17248..18054
                     /locus_tag="MB022_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466293.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18066..19286
                     /locus_tag="MB022_001286"
     CDS             18066..19286
                     /locus_tag="MB022_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466294.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFTAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19276..20862
                     /locus_tag="MB022_001287"
     CDS             19276..20862
                     /locus_tag="MB022_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4466295.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            20901..23039
                     /locus_tag="MB022_001288"
     CDS             20901..23039
                     /locus_tag="MB022_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4466296.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(23407..24390)
                     /locus_tag="MB022_001289"
     CDS             complement(23407..24390)
                     /locus_tag="MB022_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBT4466297.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7786 a         4221 c         5105 g         7640 t
ORIGIN      
        1 tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct aaactgctat
       61 gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat
      121 gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa ttgattacta
      181 aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag attcgaatta
      241 aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt tcttaactct
      301 agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga tgacttttta
      361 ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa agtaccatat
      421 aaaaaaacga ttacgtccat ataattgtgt aaaagtgaag catcaaaaaa gccattgatc
      481 tcagttagga gtacaatgtt ttttgcagaa aacatactcg aataggactg atatcaatga
      541 ctcaagtaca ttttactttt gaaagcgaag aaattcaagc aattataaac gaaagcggtg
      601 cgaatgatac cgcaaaaaca cttatgacta tcatgtttaa tcagctgatg gaagaacaac
      661 gaaatcaata tattcaggca aatgcctatg aacgttcaga agaacggcaa tcacaaagaa
      721 atggttacta cgatcgttcg tttacaactc gaattggtac gttagaactt catgttccac
      781 gaaccagaga cggtaagttt agtcctacta tctttgaaag atatcagcga agcgaaaaag
      841 ctttgatcgc tgccatgatt gaaatggtca tttctggtgt ctccactcgc aaagtaacta
      901 aaacagttga actgctaacg gatggtgcga ctgtttctaa atcattcgtt tcaaatctga
      961 tgaaacagtt ggatccattt gtttttgagt ggagaaaccg gagcctagaa ggctcggaat
     1021 atccattttt catgtgtgat gccctgtata tgaaagtaag ggaaaatcat cgtattgtct
     1081 caaaaggtgt atatattggt atcggcattg attctgatgg acgacgtacg attcttggtt
     1141 ttgatgttca agacggcgaa tcggaagata attgggatac cgtttttcaa tcgttcgttc
     1201 aacgaggttt gttcggtgta aaactggtca tttctgatgc acacaaaggg ttagttaagg
     1261 ctgttcgcaa gaacttttta ggtgcgagct ggcaaagatg ccaagcccac tttttgagaa
     1321 atatttttga taagctgcca aagaaagttt cttctgatgt aaaagacgag ttgaagagta
     1381 tctttaaagc ctctgaactt gaattgacac gtgaaagaaa agagcacttc ttggaaaaat
     1441 atggttgtga ttcaaaatta agtgctgctt gcgacatttt agaaaacggc tttgaagatg
     1501 ctattcaaat tttgtctttt cctgaaaata tccgccgaag gattcgtaca accaatgtgt
     1561 tggaacggtt gaacgaagag attcgccgga gagaacgagt gattcggatt tttccaaata
     1621 tcaattccat cactcggata attggaacac ttttgatgga aaaagacact gagtggctgg
     1681 cttctccacg aaaatattta gaatttaatt cgaataacat ttagcaccat gaacctgtgc
     1741 gagtctcttc tttctgagga gagacatcaa gagtcgctgc gctactgctc ctgacctctc
     1801 tcctcagaaa gaaaggcatg gcagttaggt tcacaacgca aaacattgtg cttttgatga
     1861 ggttgaactt ttacacaaga ttgtggactt gactaaaaaa acggtggggt tgtttagcga
     1921 aataaaggat ctaagccaat agcttagatc ctttattcta tgctttatat tattttttgc
     1981 atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta ctgtagagcc
     2041 atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat ctacgtgggt
     2101 ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg taccgccttt
     2161 cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt catcaaataa
     2221 tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta caagtgcgac
     2281 ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga catcttctcc
     2341 ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca tacccgagat
     2401 gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta ctcgatgact
     2461 gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt ctccacgagc
     2521 attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa tgatatctgt
     2581 accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt ctgctttttt
     2641 tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat agacacgagt
     2701 cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca gttctgggaa
     2761 gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga ctttatttgc
     2821 ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat aagacgctac
     2881 tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc tgatcctgct
     2941 tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta cattctcaat
     3001 acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag tccattgaac
     3061 gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag caaggggtgc
     3121 ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta cgccagtttc
     3181 tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca taataaaaat
     3241 tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta tgattcaaac
     3301 aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc atgatatatg
     3361 ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa
     3421 aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta aggagattaa
     3481 aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct gcttatgaag
     3541 tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac aaaatgaatt
     3601 gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga caagctcttt
     3661 ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat acagggaata
     3721 cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag aagttgtatc
     3781 gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc tcttatactt
     3841 actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa gagcagatcg
     3901 aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg atgtcttcaa
     3961 gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt atagatgcta
     4021 tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt ccacaaatca
     4081 gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca gcgtatgcat
     4141 ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat acagattgaa
     4201 taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag ttctttagcg
     4261 aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt aacattttcg
     4321 gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac cgatttgcag
     4381 aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc caatagatcc
     4441 aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc atcatcgtat
     4501 gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc tacttgggta
     4561 cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac aatatcggcg
     4621 atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc catgatgtgc
     4681 ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt gaaaagaaaa
     4741 taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct atgttaaaat
     4801 gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc acgtgaaatt
     4861 gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct gcgtgatttt
     4921 ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta tatcgagagt
     4981 catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac ttgtatcagt
     5041 ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg
     5101 gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg ttgggcctat
     5161 gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa gaaagctttg
     5221 tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg tcatgctatc
     5281 caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg tcatggcgtt
     5341 ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg aaaagggttg
     5401 cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg aacttggaaa
     5461 atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact ttcttgccaa
     5521 tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc acaaggagaa
     5581 gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga aagtttggaa
     5641 caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa agcgcatcac
     5701 tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct tgtctttatt
     5761 tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc aagagactgt
     5821 actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg gtcctgcaat
     5881 caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat tagcggcttt
     5941 atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt
     6001 agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt
     6061 catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga ttttggatgc
     6121 tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg
     6181 gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa ttgtccctaa
     6241 tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg tcggctggat
     6301 gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga tcagcggtta
     6361 tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg ctacgatcat
     6421 cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa
     6481 aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat tttctcataa
     6541 aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat aataattatg
     6601 actcttaatt aaagtaggtg cccatatgct tgtttctttt agtatggtta taagattttt
     6661 tctgacaatt ttattatcac tgatattaac acctgtattc aaaatcattt cagtacaaac
     6721 aggtatggtc gacaaaccaa atgaaagaag aataaataaa gttccaatgc cttcagcggg
     6781 cggactgcct atctttattt catttgttat atcgacttta ttcttattcc gtaaaattat
     6841 ccctcaatcg tatattgtac cgatcttgat tgcttcttta gtaatcatag tcacaggagt
     6901 gctcgatgat aaatatgagc tgacacctaa acaaaaaagt atagggatct tgattgcttc
     6961 tcttattatt tattttgtag cagatatacg tattgattcc ttcacgcttc cttttattgg
     7021 atatatccaa ctaggctggc ttagttttcc cgtaacgatt ttttggatct tcggtatcac
     7081 gaatgcggtc aatctgattg acggtttgga tgggctggcg gcagggattt cattgatcgg
     7141 tttaattacg ataggaatca ttggatattt tttcctccat gcttctacag tgtatattcc
     7201 agttgttatt ttctgtcttg tagcaagtat tatcggtttt tttccttaca atttttatcc
     7261 ggcaaagatt tatttaggcg atacaggagc tctattccta gggtttatga tggccgtcct
     7321 ttcactacaa gggctgaaga acgtgacttt tgtttcatcg atttctttgc tgatcgtcat
     7381 gggagtgccg gtaacagaca cgttttttgc tatcattagg agaaaagcaa accgtgtttc
     7441 tttttctaca gcagataaaa aacatcttca tcaccgacta atcagtttag gctttactca
     7501 taaaggtgct gtcctgacga tttatgcgat ggctttgatg ttttctttca cagctatggt
     7561 catgaattat actggaagaa tcggaacgat catattgatt attgccatgt tgtttgctgc
     7621 aattttatta tttgaattga ttggtctgat caatgagaaa catcagttta ttttaaaaac
     7681 attgcgattt ttagggaata aagagtaccg tacgcaagta aaacagaaat atttgaagaa
     7741 atggaaccgt catatagatg aataacaaat taaaccagcc gatccttatt agtattgtga
     7801 cggcaaacag tgataaaatt tttcggacac tagataattt tatttcaact gtaaaagatg
     7861 aaacagcagt aaaaatccga atttttgaca acaattcact tcccgagtac aaaaaaagac
     7921 taacagagta taggaaatat ccctacatcg atatctattt tcatgatgaa aattccggat
     7981 ttggttatgg acataatcac aatctgatag ctagcgattc tcgctatggc attatttgta
     8041 acccagatat tttagtggat gaagagacag ttacagcatt ggtcttgtta ttgaaagaac
     8101 atccggaatg tgcgatgtta gcacctaaga ttcttaatga agatggaaca actcagcacc
     8161 tcatccgtga gcagctggct gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg
     8221 tcaaaaaaaa gtttgacaaa cgactggctg tgtttgaatg ccgaggattg cctactgatg
     8281 ctaatagtta tgtacggatg atctcaggta gttttatggt agctgatatt gataaattta
     8341 aagagataaa ggggtttgat gagcgtttct tcatgtactt tgaagacaac gacttgtgtt
     8401 tgagttttga aaaagcaggt tacaagttat tgtacacacc cttttatgca gttatccatt
     8461 tttacgggaa gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg acttctatga
     8521 tgaaattttt caataagtgg gggtggcgtt ttttctgatg aaaccagaaa tattagtagt
     8581 cgttgtgctt tatcagcaac tattcagtca atctccagct tatggaccat taagcaaagc
     8641 acttagcgaa aagcaaattc agcttattat ttacgataat agtcctcaaa ggcagcaaaa
     8701 tgagctgttt gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc ctggtttagc
     8761 ggtggcttat aattttgcgt tgtctcaagc agatgaagga acacgttgtc tcgtcactct
     8821 ggatcaggat acgaggctag tcgacgatta ttttgaacta ttgcgtaaag taacctttac
     8881 agatgaatgt gttgctgcag ttccgatgat tttttctggg agccgacaag tttctcccct
     8941 atattcagat cgttatatca atcgtacagc tcaagcagta gaagttgaag gcccaacagc
     9001 tgaacgaatc atggcaatca attcaggtgt tgcctggtct gtcaaatttc taaaaaagat
     9061 tggcgggttt aatcctgctt tttctttaga ttttttggat cattggctgt tttggaaagt
     9121 ggatcagttg gaaaaaactg tggagatcct tcctgtacga atggaacatg atctttcggt
     9181 tttagactat gaaaaagttt caagtaatcg ataccgttct attttacaag cagaaaatcg
     9241 attttatcat gaatatgatc aaaggcattt atccagtcac cgacgacaat tattgttaag
     9301 aacagctaaa cagttcttga ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc
     9361 atatgttaag aattggaagg tgtgaattcg tgcgttcggt ttgtatagct acctttaatg
     9421 gaggcagata tttaagagag cagttagata gtattttatc tcagctttca gcagaagatg
     9481 aagtagtcat ttctgatgat gggtcagtag atgatacatg ggatatatta actgaatacg
     9541 cagaaaaaga taagcgaatc cagctttttt cggggcctcg tcaaggattg atcgctaatt
     9601 ttggttatgc gatccagcag acgaaaggag atctaatctt tttggcagac caagatgatg
     9661 tttggttgga gacaaaagtg aaaaagatca ctgactattt tgagcagaat ccggatcagc
     9721 tagtagtagt ttctgattta atcattgtgg acagcgagct gcaagagatc catgattcgt
     9781 atttttcgtt tcgaaattcc aaagaaggat ggcttaataa tgtgataaga agtcactata
     9841 ttggtgctgg tatggctttc cggtcttctt taaaacccga tattttgccg atcccgtcag
     9901 aagtgccgat gcatgatatg tggattggtt tgctgggagg aaagaagact gggttcctaa
     9961 gagaaccgtt aactctgtac cgaagacatg atttaaacgc gagtgagatt gaaacacatt
    10021 ctaaattacg gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa
    10081 aatggtttaa acgttgataa aagatgttta aatccttaat atttccatga caaatataac
    10141 aattgatgag ataataacaa agtgaaattt gaaatcaaaa aggagagcag atatccatga
    10201 agggaatcat tcttgctggg ggcagcggaa cacgtctgta ccctttaaca aaggcaacat
    10261 cgaaacaatt gatgccgatt tatgacaaac caatgattta ttatccaatg tctactctga
    10321 tgttggcggg aatcaaagaa attctgatta tttcaacgcc acaagataca ccacgtttca
    10381 aggaattatt tggaaacgga aatgatttag gactgcagat cgaatatgca gtacaagaaa
    10441 gtccagatgg attggcacaa gcgtttatca ttggagaaga atttattgga gatgatagtg
    10501 tctgcttggt attaggggac aatatttact atggtggcgg tctgtctaaa atgttgcaac
    10561 gagcagcttc aaaagataca ggcgcgacag tatttggcta tcatgtcaat gatccagaac
    10621 gctttggtgt agtagaattt gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg
    10681 ctcagccaaa atcaaattac gctgtaactg gcctttattt ttatgacaat gaggtaattt
    10741 ctatcgctaa aggtatcaaa ccttcagaac gtggagaact agagattacg gatgtcaata
    10801 aagcatattt ggataaagga aaactatccg tagaacttat gggacgcgga tttgcttggc
    10861 ttgatacagg aacacatgaa tcattgcttg aagcatctac ctttatcgaa acaattgaaa
    10921 aacgtcagaa cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg
    10981 ataaggaaca attgctggcc cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt
    11041 tattgaattt ggccgctgaa taaggagtcc ttacagatga aagtaacaga tacaaaatta
    11101 acagatgtca aaatcgtaga aatggacgtc tttggcgatc atcgcggttt cttcacagaa
    11161 agttattcaa aagcaaaatt tgcagaacat ggattgaaat atgactttgt tcaagacaac
    11221 cattcactat cagctgaagc tggtgtcctt cgggggcttc atttccaaaa aggagaagct
    11281 gcacagacaa aattgattcg ggtagtcact ggagcagttt tagatgtagt agtggatatc
    11341 cgcaaaggta gcccgactta tggtcaatgg gacggattta tcttatctga acataatcat
    11401 cgacagttgc ttgtacctaa aggatatgcc catggattcg ttactttgac accaaatgtg
    11461 aacttccttt ataagtgtga taattactat aatgcagaag cagatggggg catcgcattt
    11521 gatgatccag atttagcgat tgattggcca attgatccat caagagcgat tatgtcggaa
    11581 aaagatcacc gtcatccgac attaaaagaa tttgaagcag aaaatccatt tgtttacgga
    11641 gaaatctaag aattaaggag aaattttctt atgaaaaaca tcattgttac cggaggtgcc
    11701 ggattcatcg gctctaattt cgttcattac gtggtaaaca atcatccaga agtgcatgtc
    11761 actgttttgg ataaactgac ttatgcagga aacaaagaga acttggctgg acttcctagc
    11821 gatcgcgtag aattggtcgt aggagatgtt gcagatgcag aattagtgga ccgtctagta
    11881 aaagaagcgg acgcagttgt tcattacgcg gcagaatccc ataacgataa ttcattaaaa
    11941 gatcctttcc catttgtaca aacgaatatc attggtacgt atacactttt agaagcttgc
    12001 cgcaagtatg atgtacgcta tcatcacgta tctactgacg aggtttacgg cgatcttcca
    12061 ttacgtgaag acctgccagg acatggggaa ggcgaaggag aaaaattcac tgcggaaaca
    12121 ccatataatc cttcaagtcc atattcttct acaaaagctg gttcagatct gttagtaaaa
    12181 gcatgggtgc gttcttttgg cttacgtgca accatttcaa actgttcaaa caactatggt
    12241 ccttatcagc atattgaaaa atttattcct cgccaagtaa cgaatatctt aagcggtatt
    12301 cgtccaaaat tatatggcga aggaaagaat gttcgtgact ggatccatac gaatgatcat
    12361 tcttcagcag tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt
    12421 gcagacggtg aagaagataa taagaccgtg atggaattga tcttagaaat gatgggtcaa
    12481 ccaaaagatg cgtacgacca tgtaaacgac cgtgcaggac acgacttgcg ttatgcaatc
    12541 gattcgacaa aattacggga agagttagga tggaagcctg agtttacgaa cttccgtgat
    12601 gggttggctg acacgatcaa atggtacgaa gaacatgaag actggtggaa aaaagaaaaa
    12661 gaggccgttg aagcagcata tgccaaaaat ggacaataat acagagaaaa tgtcactgta
    12721 ttaatggaaa aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca
    12781 aggagcggat aatcatatgt ttttattaac tggaggaaat gggcaactag gtacagaatt
    12841 gcgccattta ttagatgaaa aaaatctgca atatgtttca acagatgcaa aagacttgga
    12901 tatcacagat gccgagaaaa caatggctta catcacagaa ttaaaacctg atgttatttt
    12961 ccattgtgca gcttacacag cagtagataa agcagaagaa gaagcaaaag aattagatga
    13021 gaagatcaat gttgatggta caagaaatgt agcacttgca gcaaaagctg tgaatgctca
    13081 attcgtctat atcagtacag actatgtttt cgatggaaaa aacaaaaatg tagaatacga
    13141 agtagacgac caaacgaatc cactaaatga atatggtcgt acgaaattat taggcgaaaa
    13201 agcagtagaa gagattttag aagactatta tatcatacga acttcttggg ttttcgggat
    13261 ctatggacac aatttcgtct tcacgatgca acgcctagcg gaaacccgcg acaaactgac
    13321 tgtagtaaat gaccagtttg ggcgaccaac ttggacacgt actttggcag aatttatggt
    13381 atatgtcatc gaagaaaaag caccatttgg tatttatcat ttgtcgaacg aaaatagctg
    13441 ttcttggtat gaattcgcaa aagaaatatt gaaagataca gatgtagaag ttgcacctgt
    13501 tacgtcagaa gaatttccgc aaaaagcaac acgtccgcag tattcagtca tgagcctgaa
    13561 aaagacagaa gagttaggat ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct
    13621 ggaagctgcc aaaaaaatga ataagtaatt tccttttatc aaatagaatt tggagcatga
    13681 tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga accaggcaga
    13741 ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg ttttagtcat
    13801 cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg ataaatatgt
    13861 gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc gggaagtgcg
    13921 gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag aggactttcc
    13981 gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt ataattctcc
    14041 tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt tcctcggaag
    14101 agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct tgcttggatt
    14161 cgaagtactg atgatattaa cgaagagaaa aaaagctcta tcgctaatcg gtgcatttgc
    14221 gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg atttgatttt
    14281 ctttactttg ggactgatga ctgcttttta tcattatttc tacaatcatg ataaaaaatg
    14341 gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta ttttagtgat
    14401 ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg ttggcctgct
    14461 tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga ttggaggggc
    14521 agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag acgcattgtt
    14581 agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa agtggacgat
    14641 tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata tcaactattc
    14701 gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc tagcaagtcc
    14761 atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta ttttgatgat
    14821 tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc tagcagaaat
    14881 cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt ttgcggcttg
    14941 tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc ctatttggta
    15001 taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta cgcattcgaa
    15061 aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg gaacagtgat
    15121 tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg ccgccttagt
    15181 tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga tttttgaaaa
    15241 aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag tttggttgtc
    15301 tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca atacagtacg
    15361 cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat acgagaagta
    15421 ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa agttcatttt
    15481 agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa aattagggat
    15541 cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg aagcgcagtt
    15601 cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag aatatccgga
    15661 ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac agtaataggc
    15721 tagctgagaa taatcctgaa atgttagtag aaaggagagt ggagataatt caagcaatcg
    15781 tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttactg acgctagtct
    15841 tttttgtttg gacagcaggg ttaggttttc cagcaacttt gtttatatat aacaatagtt
    15901 cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat aaagcaaaag
    15961 gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt tcttactttc
    16021 gggaattaag aaatgagtct ttatttggag acttactgtt tcctttgatt attttatttt
    16081 gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat ctgattgtct
    16141 tttcggtctt cctcggaatc acgtttatcg tgtatttaca gagatccaag taccagaagg
    16201 acaaagcatc tgggctccag gcaattcgct ttctgatatt tggatcaata cgaatacaat
    16261 cggaagttct ttgatgactc ttgctttatt gatcagtgga tttgccagtt cttttgaacg
    16321 atggtatgta cgaatcatcg gtgcagtagc agttgttgca gcttttctcg ggatttgggt
    16381 ctgccaatct agaggtgcat tagtcgcgtt gattgttttt gcgatattgg atcttttacc
    16441 taaatcgttg atgcgtgtca ttcgtgcgcc atttattgct tacacggtta cgattctttt
    16501 agctttgccg atttcttatc tggcggctgt ctctgaaaaa gtcaatttgt ttaccggtcg
    16561 agaagatatt tggcataaat tctatcagac gctaggagaa aaatctgaac aaattttact
    16621 aggaatgaaa acatttatct tccaacgagg aaaccaattt ttaggaaacc acaatagtta
    16681 taattctatt ttgaatatct atggattgat aggatttggg attgctgctt tgctgcttat
    16741 cctttttatt ggtcgcttaa cgttgaaagc agatttatcc aacgggcaga tgacttttat
    16801 ctgggccttt tttgcggtga tgatgcagtc atttatggag gatacattga cttcataacc
    16861 atgggttccg attatttata tcctgttggc tatggccgtt catcgatatg atgaaccaga
    16921 agttgcggag gaattctatg ttatggaaga ggcatatgac gatatgactt atgatgatac
    16981 atcttatgag gaacaaccag atagcgctca ttcttccagg atccaacgct atcattgata
    17041 ggcacaaaat acgagagagg ctgtgacaac cgtcgcagcc ttttttggtt tattgcaatt
    17101 gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc ttgtatcata gaaaattaag
    17161 aaagaaataa gaacgcagct cggtaccaaa aatatggtaa aatggcactt gactgtaatt
    17221 cactgaaaat cgcttggagg aacacaaatg tttaaagatt tgtatttatt tatgaaggac
    17281 atttatcaga acagaaaatt attgctacag ttttcgatca atgacttcaa atcaagatat
    17341 gcaggatcgt ttttaggaat cttatgggcg tttatcaatc cgctatttac tgtattgatt
    17401 tattggctgg tatttggtgt tggactgaaa gcacaaatga tcgatgcaaa atacccattt
    17461 atcgtatatt tagttaccgg gatgattccc tggttcttct tctcagatgc atttgcatca
    17521 actacggttg tttttcggga atatacttat ttagtaaaga aagtcgtatt caatattcgt
    17581 atcttgccca cgacgaaaat cctatccaat ctgtacaccc atttattctt cattctgatt
    17641 ggcatagctg tcgcgcttat aaatggtata tatccttcag caatgtcttt acagctgatt
    17701 tattatttat tttgcatgtg tgctttcttg acaggattga cttggctgac ggcatcgatc
    17761 caaccatttt tacctgatat catgcaatta attacgatcg tgctgcagtt gatcatgtgg
    17821 acattaccga tattatggag ccctaatcag tttgattcag gtcttgtaag catattgaaa
    17881 ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt catttttaag tgaagcttgg
    17941 ttctgggagc atggaacata tactttgtat ttctgggttt tcacgttagt aatgttcgta
    18001 atcggctctg cagtgttccg tagattgaaa ccacattttt cagatgtgct gtaaggagaa
    18061 tagctatgac agaatatgca attagactaa aagatatcac aaaatcttat aatatgtacg
    18121 caaaaccttc ggatcgattt cgtgaagccc tcaatccttt taagaaatcc tatcatgatg
    18181 tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa aggtgagatg atcggtttcg
    18241 tgggagaaaa cggatcggga aaatctacaa tgttgaaaat cattacggga gtactgacac
    18301 caacatccgg cacgatggaa atcgaaggaa aaatctcagc tttgctagaa ctaggatcag
    18361 gattcaatcc tgagtattct ggctatgaaa atatctattt aaatggaatg gttctaggat
    18421 tttcaagaga agaagtcgat gaaatggtcg atgatatcat tgaatttgct gacatcggag
    18481 accatattta tcaacctgta aaaacttatt caagcgggat gttcgttcgt ctagcttttg
    18541 ctgtagcaat caatgtgaat cccgatattt tgattgtgga tgaagcattg gcagtaggcg
    18601 atcttgaatt tcaactaaaa tgtatggaaa aatttacaga aatcaagaac tcgggaaaga
    18661 cgattttatt cgtttctcat gatatcagtt cgattcgtcg tttttgtgat cgttcctatt
    18721 ggttgaaaaa cggagaagtc gtcgaatatg gcgatacgat ggaagtaacg acgaattatg
    18781 aaaacttttt aaagaaaaaa tcagttaaaa cagtggatcg gaataaaaac caagtagaac
    18841 ggttcaccgc tccagatatc gtcgacgtga cgaaagcgga attgttggat aaagacaaaa
    18901 atccggtgga tatgctggaa caaggagaaa aactatacgt taaagtaact tatgaagtaa
    18961 aagatgatac aatcaaaaat ccggttttag gaattgcgct acgaacagtc gacaattttt
    19021 atgtatgcgg acttaatact ctactagatg gtatcaagat tccttggaaa aaaggagaga
    19081 atatctttta tctggagtat gaaaagttag gtttgttagc tggtgaatat tattttgacg
    19141 ttgcggtctt tgaagaaaat gcaacggttc ctttggtata taagacaaag tacatgaact
    19201 tgtttgtcag cggttcttat atcggagaag gcatcgtggt cttagaccat aaatgggaag
    19261 aagggactca tagcaatgaa atatgatttt gaaatggacc tagatgaaca aagcagtgtg
    19321 ggaaaaattg ccgcacagat caaaccagga agtaaagttt tagagtttgg tcccggaaac
    19381 ggccgattaa cgaaacattt aatcggagca aaacaatgtg aagtgagcat tgttgagtta
    19441 gataaggaat tgttcgattt tgtcagcgaa tttgctcaag atggatttta tggtgatatc
    19501 gaaagttttg agtgggcaaa ctattatgct ggacagacat ttgattacgt cttgtttgcg
    19561 gatgttttag aacatttggt agatcctgga aaaacattga agaaagtcag agaatttttg
    19621 aatgaagaag gagagatcct gattacattc cctaacttgg cgcataattc tgtcatgatc
    19681 gatttgttca ataaccaatt gccgtgggct tcttatggtc tcctagatga aacccataat
    19741 tctttttata ctcatgatgg cttccaaaaa gtttttgaaa aagcgggatt attcattaat
    19801 attgaagatt atttgtactt agctgtaggt gatacagaat taaaatcaac ttatgaagaa
    19861 ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat ttggtgaagt ctaccaatac
    19921 tttttctcat tgatgaaaca tccagtagca caaagttcca ttgcagagcc acaaaattct
    19981 aattatgtaa aagtgttaga agtaacccag caaacgaaac aagatgaaac aatccaacaa
    20041 ataccattca ataatttcac aggggaaaat gagacactaa gtttccccgt atcagaaaca
    20101 acagaaagaa tggtgtttcg cttcgcgcaa caacctagct ttatcgaatt cagcgcagaa
    20161 gctgccggag aaaaactaac atttatcgat tcgaatgctg tagtcaaaac agtaaatgat
    20221 tgttatttgt ttgatgggaa agaattacca gagttcgttc taacggatat ttctgggaaa
    20281 gaagtgacta tccattgtca ctatcgcttt atcggggaat tgacaccaac aatgaaagaa
    20341 ttgttggaaa cgattcgtcc catggctgaa gtcacaaagc agctttctga gaaaaatgac
    20401 gaattagaaa gagaaaatca tcatcttctt gaggaaaata caagactaga ccatacgtta
    20461 aaaacaacca caaatcgcta ttgcacattg ttggaaagtg atgaatggac aataaaacat
    20521 agactaggac gacgtaagaa agaaacttcg aaaaaaatcc aagaaaaaga actttctata
    20581 tgtattgacg agaaaatctg ggatgcagaa acaaaaatat taaaaatcat cggatgggga
    20641 atcgctaatt cggatcgcca gcccttgtcc tataagttat cagctgacca atcgccattt
    20701 ttcgaagcaa ttcctgtttc acgagaagaa gtcaatcaag cagaacagct cgcaaaagga
    20761 acagaagcgg gatttgagtt acgaatcctt tgcgagcaag aacgttcatt cttagtggaa
    20821 gcagtagctc aaaatggcca atcatggatc attgaaatgt aaataatgat taacttataa
    20881 agataaggag taaacgaatc gtgcaaaaaa aagaaattgc cgtatttatc gatcaaatca
    20941 ctcgtgaacg ggccactggc gatttgctta ttgtgggctg ggcaattgat gaagtgacca
    21001 aagaaatccc tacgataaag gtagaaaaag aaaatgtgat tgctgaagca acacatgtcg
    21061 taagattgga tatcaatcat ttatataatc ttgatgttaa gacccaaagc ggttttaagg
    21121 tacgtttatc agggaaaatg agaggaaaag ccatcttaga cttccaaaca gcgagacatc
    21181 aaaatggcat agctgtgaaa ttaaacggta aataccctta tgatgacggc attgaatctt
    21241 catgggaaag aaaaaaaaga ttactgaaaa aagggataaa ttatgcaagg actcatggcg
    21301 taaaaaaagc gatccgtcgg gtcaaattgg aattgaagcc aggatcgatc gactatgcag
    21361 agtggatcag tcgtcatgag aaaccagatt tgaaagagca aaggaaagaa gcacaaggat
    21421 ttgcgtaccg tccattgatt tccatactta tgcctgtcta taacgttgaa atcaaatggc
    21481 tcgaaaaatg cattgattcg gtactggatc aaacgtatga tcattgggaa ctatgtatca
    21541 gtgatgatgc ttctactgat ccagctatcc gaaagtgtct ggaatcctat caagcaaaag
    21601 atgatagaat caaagtcgtt ttccgaaaag aaaatggaca catcagtctg gcaactaatt
    21661 ctgcattgga aatagcagaa ggagaattta tagcactctt agataatgat gatgaattac
    21721 cgccatttgc attatatgaa gtggcaaaag tgctaaacgt acatccagaa ctggatgtga
    21781 tctacagtga cgaagataaa atcgatgcag atggcaatcg ctttgatccg catttcaaag
    21841 cagactggtc gcctgataca ttgatgggaa ataattatat ctctcactta ggcgtatatc
    21901 gaacaagcat tgttaaagaa cttggcggtt tccgtaaagg ctatgaaggt tctcaagatt
    21961 atgatttggt ccttcgtgtg acagaacaaa tccctgcaga tcatatttac cacattgacc
    22021 gagtcttgta tcattggcgg acgatcccag gatcaactgc aagtaatggc gaagccaaaa
    22081 gttatatcta tgattccgga gtgaaagcat tgacagatgc cctgtctaga agaaacatca
    22141 aaggaagcgt acgtcctggc agaatttctg gattttatga gattgcttat gacgtcttgc
    22201 aagaggattt ggtcagtgtg atcattccaa cgaagaatgg ctatgaagat ctaaaaactt
    22261 gtgtggattc gatcatcgaa aaaacgtctt attcaaatta tgaaatcatt attgcggata
    22321 acggaagtac agatccaaaa atgcaagaat tatttgcaga atacaagcat cagctgaaag
    22381 accgctttat cgtagaattg attgacattc cattcaatta ttctcgaatt aacaatttag
    22441 cagcagaaaa agcaaacggc aaatactttt tattcttgaa taacgataca gaagtgattg
    22501 agccagattg gatgacggtg atggtttctt atgcacagtt tgatcgaatc ggctgtgtag
    22561 gagcaaaact gttttatcca gatgatacaa cgcagcatgc aggagtatta ttaggaatcg
    22621 gtggtgttgc agggcatgca ttgaataact atgatcgaac acattgtggt tatttcggcc
    22681 gattggtcat tgacgtcaat tacttagcag tcactgcagc ttgtatgatg gtcaaggcag
    22741 ctgacttcaa tgctgttaac gggtttgatg aaacattaga agtagcattc aacgatgttg
    22801 acttgtgctt gaaagtttat gaactgggtc gttacaatgt ctatgcacat caagcagaat
    22861 tgtaccattt cgaatctaaa tcaagaggtt atgaagatac tccagaaaaa caaagacgtt
    22921 ttgctggtga aatcaaaaaa atgcaagata aatggccagc atatattgcg catgatccat
    22981 tttacaatga taatttaaca aaagaaggaa tcggcgactt ttcgttgaga ccagattaaa
    23041 ctataataaa aagctgttat gttgtgattc gctcgcacat aacggctttt tatagttttt
    23101 tgtcaaaaaa aggcagaagg tgccgactca aaaaaataag tcgaggttgg gacagaagtg
    23161 tttaactccg agaaataaga agaaattcac gaaaattgct tttcaaattt ttgtgaattt
    23221 cggcttattt aagaagaagt tgcttctgct cccaccgttt attcgttttt aaagaatgag
    23281 acaaaagtgt ttcttacttt tgtcccacgc tcttattcat gctcagagcg ccaatagata
    23341 caaaatcata gccaaagttt cacgaaccct agctgtcaaa gaatcgatat tttattttaa
    23401 tataggctat tttaaaataa atggtttaag gtattcctct acaaaagcca ctctatcaat
    23461 tatcatgggt ttaaaataag aagtgatgga gataaccaga ttgttcttag cgtcaatata
    23521 aagaacattt ccactattac caattgctga atatatttcg ttctcagaat ctaataccca
    23581 ccataaatag ccatatctca tattttgtaa gtcttctaaa gcactggttc taggtttgaa
    23641 catctcgttt atccacatgg gtggtacgat ttgttttcct ttatacatcc ctttatttag
    23701 gcataataca ccaatttttg ccagatcact tgccgataaa cacaaaccat atcctgccgt
    23761 cccgatattt agtggatcac aaaaccagac attggtttta ggagacttat ccattgtaaa
    23821 ctgcttatgt tcttcagctg tttcagcata ataattgaca tggtttggta tccctatagg
    23881 atcaaataga tacttattgg caaaatcaac agttgttagc ttacttgtct gatacaacaa
    23941 gccagttaat atgtgtaaac agacagtttg atagttgaat tcgccggtta taccttttct
    24001 tcctcccaat aaatctagtg atgtttttgt ccaatcttca cttgaacaga cttttgtcca
    24061 tggatcgcct ttacatttgt agggtgcttt cattgttagc aagtgcttga ttgtaatagc
    24121 gtaaatagtt ttctccccgc gtttcacttt atactctgga aaataatcta atactttatc
    24181 atcgatactt ttgattaatc cttgctctaa agcaatgcca atcagcaaag atacaatact
    24241 tttcgttgct gacatgatat gacaagtatc gtctttttta taaccattcc attcatctgt
    24301 ataaacttct accccatcct tatatgcgac aatttgacat atatttggtt gtcgaaaacc
    24361 aataaaatca tgcaactctt gtttattcat aagatattac ctcactttct tctataagca
    24421 taatgtcatt aaaaatcgat ttcaattttt tcgattcttc acgtaataca ggtattctta
    24481 tggactagac agcgctttgg gaagatggat tctctgaaaa atataaaggt tcactttatg
    24541 agtggataaa aaaagcagca aatacttcga cttctttaat ttaccaacat tagtgaaaat
    24601 cgattcatct ggagatttgt aatttagagt ccttgtcttg attagaggtg tataaagctt
    24661 tttattagac atttttaaat tccattacaa tttgcttata aactaggttt ttaaaggtga
    24721 ctatggtaag ataatagaga tattattttg tg
//
LOCUS       DAGCXG010000031        24442 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGCXG010000031 DAGCXG010000000
VERSION     DAGCXG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.032"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(139..987)
                     /locus_tag="MB022_001290"
     CDS             complement(139..987)
                     /locus_tag="MB022_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4466298.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1211..3151)
                     /gene="thrS"
                     /locus_tag="MB022_001291"
     CDS             complement(1211..3151)
                     /gene="thrS"
                     /locus_tag="MB022_001291"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBT4466299.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3488..3667)
                     /locus_tag="MB022_001292"
     CDS             complement(3488..3667)
                     /locus_tag="MB022_001292"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBT4466300.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3714..5537)
                     /gene="cadA"
                     /locus_tag="MB022_001293"
     CDS             complement(3714..5537)
                     /gene="cadA"
                     /locus_tag="MB022_001293"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4466301.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5815..8094
                     /locus_tag="MB022_001294"
     CDS             5815..8094
                     /locus_tag="MB022_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4466302.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8187..8834)
                     /locus_tag="MB022_001295"
     CDS             complement(8187..8834)
                     /locus_tag="MB022_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466303.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9013..9732)
                     /locus_tag="MB022_001296"
     CDS             complement(9013..9732)
                     /locus_tag="MB022_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4466304.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9863..11014)
                     /locus_tag="MB022_001297"
     CDS             complement(9863..11014)
                     /locus_tag="MB022_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBT4466305.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11231..11971)
                     /locus_tag="MB022_001298"
     CDS             complement(11231..11971)
                     /locus_tag="MB022_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4466306.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11975..12298)
                     /gene="rsfS"
                     /locus_tag="MB022_001299"
     CDS             complement(11975..12298)
                     /gene="rsfS"
                     /locus_tag="MB022_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBT4466307.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12355..12948)
                     /gene="yqeK"
                     /locus_tag="MB022_001300"
     CDS             complement(12355..12948)
                     /gene="yqeK"
                     /locus_tag="MB022_001300"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBT4466308.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12932..13582)
                     /locus_tag="MB022_001301"
     CDS             complement(12932..13582)
                     /locus_tag="MB022_001301"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBT4466309.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13592..13903)
                     /gene="yhbY"
                     /locus_tag="MB022_001302"
     CDS             complement(13592..13903)
                     /gene="yhbY"
                     /locus_tag="MB022_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBT4466310.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13919..15028)
                     /gene="yqeH"
                     /locus_tag="MB022_001303"
     CDS             complement(13919..15028)
                     /gene="yqeH"
                     /locus_tag="MB022_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBT4466311.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15025..15552)
                     /locus_tag="MB022_001304"
     CDS             complement(15025..15552)
                     /locus_tag="MB022_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBT4466312.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15732..16517)
                     /locus_tag="MB022_001305"
     CDS             complement(15732..16517)
                     /locus_tag="MB022_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBT4466313.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16510..17379)
                     /locus_tag="MB022_001306"
     CDS             complement(16510..17379)
                     /locus_tag="MB022_001306"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBT4466314.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17380..18759)
                     /locus_tag="MB022_001307"
     CDS             complement(17380..18759)
                     /locus_tag="MB022_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBT4466315.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18767..19186)
                     /gene="fabZ"
                     /locus_tag="MB022_001308"
     CDS             complement(18767..19186)
                     /gene="fabZ"
                     /locus_tag="MB022_001308"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4466316.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19211..19687)
                     /gene="accB"
                     /locus_tag="MB022_001309"
     CDS             complement(19211..19687)
                     /gene="accB"
                     /locus_tag="MB022_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBT4466317.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19690..20925)
                     /gene="fabF"
                     /locus_tag="MB022_001310"
     CDS             complement(19690..20925)
                     /gene="fabF"
                     /locus_tag="MB022_001310"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBT4466318.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20944..21681)
                     /gene="fabG"
                     /locus_tag="MB022_001311"
     CDS             complement(20944..21681)
                     /gene="fabG"
                     /locus_tag="MB022_001311"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBT4466319.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21693..22610)
                     /gene="fabD"
                     /locus_tag="MB022_001312"
     CDS             complement(21693..22610)
                     /gene="fabD"
                     /locus_tag="MB022_001312"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBT4466320.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22717..22941)
                     /locus_tag="MB022_001313"
     CDS             complement(22717..22941)
                     /locus_tag="MB022_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4466321.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22994..23956)
                     /locus_tag="MB022_001314"
     CDS             complement(22994..23956)
                     /locus_tag="MB022_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBT4466322.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23966..24406)
                     /locus_tag="MB022_001315"
     CDS             complement(23966..24406)
                     /locus_tag="MB022_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4466323.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6691 a         5433 c         4256 g         8062 t
ORIGIN      
        1 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
       61 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
      121 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
      181 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
      241 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
      301 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
      361 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
      421 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
      481 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
      541 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
      601 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
      661 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
      721 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
      781 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
      841 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
      901 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
      961 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     1021 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     1081 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     1141 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     1201 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     1261 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     1321 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     1381 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     1441 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     1501 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     1561 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     1621 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     1681 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     1741 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     1801 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     1861 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     1921 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     1981 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     2041 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     2101 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     2161 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     2221 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     2281 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     2341 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     2401 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     2461 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     2521 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     2581 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     2641 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     2701 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     2761 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     2821 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     2881 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     2941 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     3001 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     3061 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     3121 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     3181 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     3241 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     3301 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     3361 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     3421 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     3481 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     3541 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     3601 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     3661 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     3721 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     3781 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     3841 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     3901 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     3961 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     4021 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     4081 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     4141 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     4201 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     4261 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     4321 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     4381 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     4441 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     4501 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     4561 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     4621 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     4681 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     4741 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     4801 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     4861 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     4921 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     4981 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     5041 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     5101 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     5161 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     5221 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     5281 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     5341 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     5401 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     5461 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     5521 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     5581 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     5641 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     5701 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     5761 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     5821 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     5881 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     5941 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     6001 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     6061 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     6121 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     6181 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     6241 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     6301 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     6361 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     6421 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     6481 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     6541 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     6601 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     6661 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     6721 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     6781 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     6841 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     6901 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     6961 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     7021 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     7081 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     7141 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     7201 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     7261 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     7321 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     7381 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     7441 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     7501 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
     7561 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
     7621 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
     7681 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
     7741 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
     7801 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
     7861 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
     7921 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
     7981 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
     8041 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
     8101 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
     8161 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
     8221 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
     8281 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
     8341 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
     8401 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
     8461 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
     8521 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
     8581 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
     8641 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
     8701 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
     8761 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
     8821 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
     8881 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
     8941 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
     9001 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
     9061 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
     9121 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
     9181 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
     9241 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
     9301 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
     9361 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
     9421 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
     9481 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
     9541 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
     9601 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
     9661 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
     9721 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
     9781 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
     9841 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
     9901 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
     9961 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    10021 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    10081 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    10141 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    10201 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    10261 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    10321 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    10381 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    10441 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    10501 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    10561 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    10621 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    10681 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    10741 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    10801 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    10861 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    10921 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    10981 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    11041 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    11101 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    11161 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    11221 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    11281 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    11341 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    11401 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    11461 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    11521 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    11581 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    11641 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    11701 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    11761 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    11821 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    11881 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    11941 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    12001 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    12061 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    12121 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    12181 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    12241 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    12301 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    12361 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    12421 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    12481 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    12541 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    12601 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    12661 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    12721 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    12781 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    12841 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    12901 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    12961 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    13021 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    13081 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    13141 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    13201 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    13261 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    13321 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    13381 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    13441 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    13501 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    13561 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    13621 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    13681 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    13741 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    13801 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    13861 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    13921 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    13981 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    14041 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    14101 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    14161 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    14221 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    14281 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    14341 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    14401 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    14461 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    14521 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    14581 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    14641 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    14701 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    14761 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    14821 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    14881 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    14941 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    15001 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    15061 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    15121 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    15181 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    15241 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    15301 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    15361 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    15421 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    15481 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    15541 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    15601 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    15661 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    15721 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    15781 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    15841 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    15901 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    15961 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    16021 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    16081 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    16141 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    16201 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    16261 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    16321 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    16381 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    16441 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    16501 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    16561 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    16621 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    16681 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    16741 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    16801 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    16861 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    16921 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    16981 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    17041 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    17101 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    17161 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    17221 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    17281 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    17341 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    17401 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    17461 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    17521 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    17581 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    17641 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    17701 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    17761 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    17821 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    17881 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    17941 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    18001 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    18061 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    18121 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    18181 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    18241 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    18301 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    18361 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    18421 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    18481 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    18541 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    18601 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    18661 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    18721 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    18781 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    18841 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    18901 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    18961 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    19021 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    19081 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    19141 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    19201 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    19261 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    19321 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    19381 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    19441 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    19501 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    19561 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    19621 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    19681 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    19741 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    19801 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    19861 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    19921 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    19981 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    20041 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    20101 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    20161 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    20221 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    20281 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    20341 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    20401 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    20461 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    20521 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    20581 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    20641 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    20701 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    20761 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    20821 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    20881 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    20941 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    21001 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    21061 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    21121 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    21181 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    21241 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    21301 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    21361 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    21421 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    21481 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    21541 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    21601 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    21661 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    21721 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    21781 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    21841 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    21901 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    21961 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    22021 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    22081 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    22141 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    22201 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    22261 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    22321 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    22381 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    22441 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    22501 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    22561 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    22621 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    22681 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    22741 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    22801 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    22861 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    22921 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    22981 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    23041 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    23101 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    23161 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    23221 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    23281 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    23341 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    23401 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    23461 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    23521 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    23581 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    23641 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    23701 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    23761 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    23821 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    23881 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    23941 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    24001 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    24061 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    24121 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    24181 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    24241 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    24301 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    24361 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    24421 taattagttt gattatcaaa tc
//
LOCUS       DAGCXG010000032        23656 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGCXG010000032 DAGCXG010000000
VERSION     DAGCXG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.033"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            251..526
                     /locus_tag="MB022_001316"
     CDS             251..526
                     /locus_tag="MB022_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466324.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2617
                     /locus_tag="MB022_001317"
     CDS             539..2617
                     /locus_tag="MB022_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBT4466325.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2777..4663
                     /locus_tag="MB022_001318"
     CDS             2777..4663
                     /locus_tag="MB022_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4466326.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4675..5622
                     /locus_tag="MB022_001319"
     CDS             4675..5622
                     /locus_tag="MB022_001319"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBT4466327.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5642..6169
                     /locus_tag="MB022_001320"
     CDS             5642..6169
                     /locus_tag="MB022_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4466328.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6232..6885)
                     /locus_tag="MB022_001321"
     CDS             complement(6232..6885)
                     /locus_tag="MB022_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBT4466329.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7017..7859
                     /locus_tag="MB022_001322"
     CDS             7017..7859
                     /locus_tag="MB022_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4466330.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8017..8913
                     /locus_tag="MB022_001323"
     CDS             8017..8913
                     /locus_tag="MB022_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4466331.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8916..9629
                     /locus_tag="MB022_001324"
     CDS             8916..9629
                     /locus_tag="MB022_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBT4466332.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9649..10167
                     /gene="msrA"
                     /locus_tag="MB022_001325"
     CDS             9649..10167
                     /gene="msrA"
                     /locus_tag="MB022_001325"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4466333.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10164..10391
                     /locus_tag="MB022_001326"
     CDS             10164..10391
                     /locus_tag="MB022_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBT4466334.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10543..11094
                     /gene="lepB"
                     /locus_tag="MB022_001327"
     CDS             10543..11094
                     /gene="lepB"
                     /locus_tag="MB022_001327"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4466335.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11177..11677)
                     /locus_tag="MB022_001328"
     CDS             complement(11177..11677)
                     /locus_tag="MB022_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4466336.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11956..12354)
                     /locus_tag="MB022_001329"
     CDS             complement(11956..12354)
                     /locus_tag="MB022_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4466337.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12614..13474
                     /gene="ylqF"
                     /locus_tag="MB022_001330"
     CDS             12614..13474
                     /gene="ylqF"
                     /locus_tag="MB022_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBT4466338.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13467..14234
                     /locus_tag="MB022_001331"
     CDS             13467..14234
                     /locus_tag="MB022_001331"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBT4466339.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14290..15147
                     /gene="dprA"
                     /locus_tag="MB022_001332"
     CDS             14290..15147
                     /gene="dprA"
                     /locus_tag="MB022_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBT4466340.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15269..17347
                     /gene="topA"
                     /locus_tag="MB022_001333"
     CDS             15269..17347
                     /gene="topA"
                     /locus_tag="MB022_001333"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBT4466341.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17316..18692
                     /gene="trmFO"
                     /locus_tag="MB022_001334"
     CDS             17316..18692
                     /gene="trmFO"
                     /locus_tag="MB022_001334"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBT4466342.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18890..19798
                     /gene="xerC"
                     /locus_tag="MB022_001335"
     CDS             18890..19798
                     /gene="xerC"
                     /locus_tag="MB022_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBT4466343.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDDIHPHMLRHTFATHLLNNGADLRTVQELLGHAN
                     LSTTQIYAHVTKESLQKNYRSFHPRA"
     gene            19835..20383
                     /gene="hslV"
                     /locus_tag="MB022_001336"
     CDS             19835..20383
                     /gene="hslV"
                     /locus_tag="MB022_001336"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBT4466344.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20397..21797
                     /gene="hslU"
                     /locus_tag="MB022_001337"
     CDS             20397..21797
                     /gene="hslU"
                     /locus_tag="MB022_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBT4466345.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21815..22606
                     /gene="codY"
                     /locus_tag="MB022_001338"
     CDS             21815..22606
                     /gene="codY"
                     /locus_tag="MB022_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBT4466346.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22688..23563
                     /locus_tag="MB022_001339"
     CDS             22688..23563
                     /locus_tag="MB022_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBT4466347.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8193 a         4080 c         4983 g         6400 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt
     2641 ataaaaaaca aaacatatca gaacaggatc gaatcggtcc tgttcttttt ttgagaacac
     2701 aaaaagaaca aataccaaaa aaattttcct gaagttcact atttttatga tacaatatcc
     2761 aagataagga gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg
     2821 tgaaaaagat ttgtttgacc agatttcttt tttggtccac gaaaaagacc gtatcgggtt
     2881 gattgggacg aacgggacgg gtaaaacaag tctcttaaat attattgccg gtatcgatag
     2941 cagcgacggt gatcggcaaa cagtgtttta tccaactgat taccgtattg gttatctttc
     3001 tcaagcagct gaattttccg atgaattaac tgttctccaa gcagtctttc aaggaaacag
     3061 ccccttgatc caaactgttc gagcatatga ggaagcattg atcgaactag gggaaaacgg
     3121 agaagaccct gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga ataaagaaga
     3181 tgcttggact acagatacca acgcaaagat tattttgcaa aaattaggaa tcagtgaatt
     3241 agataaaaaa atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt
     3301 gttgattgat gaacccgatt tactattact agacgaacca acaaaccatt tagattatga
     3361 agcgatcgag tggttggaaa actatttgaa acaataccgt ggcgcactat tgatggttac
     3421 ccacgatcgt tattttctag atagagtagc aaaccgaatc ttcgaacttt catttggaaa
     3481 attatacgag tataaaggaa attatgaagc ttacgtctta gaaaaagcag aacgtgatcg
     3541 tgtagaagta gaacaagaag aaaagcgcaa gcggctttat aaacaggaat tagcttggat
     3601 gagggctggc gtacaggcac gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga
     3661 tctaaaggag aacttgtacc aagtaaacca agaagatgat ttagagctga acctagcgac
     3721 acaacgttta gggaaaaaag tactggaaat caaagatggc agttaccgta taaatgacca
     3781 aacactgcta gaacatttag atttattgat tcaatctcgg gaaagactag gtattactgg
     3841 taaaaatggt gcgggaaaat cgacattgct taatattcta gctggcagaa ttcccctgga
     3901 tagcggtaca atgtctatcg gtgaaacggt tcatcttgcg tattatacac aagaaaatga
     3961 agaaatggca ccagataaac ggatgattgc ttacttacaa gaagctgctg aagaagcaaa
     4021 aacagcagat ggcagtcaaa tcggagtagc agaattactt gaacgatttt tatttccaag
     4081 atttatgcat gggacactga taggcaaact ttcaggcgga gaaaaacgcc ggttattttt
     4141 gttgaaactg ctgattcagc aacctaatgt tttattattg gatgaaccga ccaatgattt
     4201 ggacatagca acattgacga ttttggaaga ttattttaga agttttccag gtgctgtgat
     4261 cactgtttcc catgaccgtt atttcttgga caaggtagca gataagttat tagtattcca
     4321 aggaaacgga aaacaagaac tttattacgg aaacatgtcc agttatttac tgaagcaaaa
     4381 agagacccag cagcctgctg aaaaagcgaa accaaaaata cagagcaagg aacctgctga
     4441 aaagaagaaa ttatcttaca tggaacaaaa agaatgggaa acgattgaag atgagattgc
     4501 agaacttgaa gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt
     4561 cactcgatta caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa
     4621 aatggcacgg tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa
     4681 caagcgtatc ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga
     4741 acaggcacag gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg
     4801 tttcctttgt taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg
     4861 tttttaaaag gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat
     4921 gaatgggcat ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat
     4981 tttggccata gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag
     5041 cttttttgcg agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga
     5101 cacatctacg gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa
     5161 atcaaacaag tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct
     5221 gcatggaatc cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa
     5281 ttttatgttc aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt
     5341 ctaggcgttc cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa
     5401 acaggtttag cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat
     5461 catatcgaac aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt
     5521 ttgaatcagg aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga
     5581 tatgacccac atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa
     5641 aatgttagct gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct
     5701 tccttggcgt ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact
     5761 agtgatggga cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac
     5821 gattgttttg acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt
     5881 ggaagacgtg cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc
     5941 agctgtttat caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca
     6001 tgtattcgat ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa
     6061 tactagtcaa ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca
     6121 gcgtaatgag aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg
     6181 atagactagc tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa
     6241 gaataaaaca gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac
     6301 atgcatatag cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc
     6361 gcctgctacc atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc
     6421 cacgacacag agccacccca taaaaatata gatgatcgta gatacttttg atactctctc
     6481 ccgtttatgc aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa
     6541 tacaaataaa gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact
     6601 tcctgcaatc aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt
     6661 tgtaaaaatc aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc
     6721 atagatagca taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag
     6781 aaccactagt cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac
     6841 ttcatttaat atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt
     6901 actaggtgta attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct
     6961 tacatgtagt ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga
     7021 caaatgtaaa aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt
     7081 taggcattcg tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg
     7141 accaactaga cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa
     7201 caagccaacc accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca
     7261 gtgaagtgat ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac
     7321 aagccagtaa tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag
     7381 gattatcttt tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga
     7441 aaatccttga agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca
     7501 cactagataa cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg
     7561 ttttgaacat acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg
     7621 ggcgaggaaa gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat
     7681 tgccaaatgt gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat
     7741 tcaaagaggg tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc
     7801 cggttatcgc tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt
     7861 cgaaaagagg ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa
     7921 aattaggaaa ataattgttt tcttttttca taaagactac ctttttgata aaatttttga
     7981 taacattaga accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg
     8041 atcctcgttt tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct
     8101 ataccgaaag ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa
     8161 aaagaaaaac ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa
     8221 accaagcaag gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg
     8281 accgcggttg aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag
     8341 cgagtaaaaa caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg
     8401 actgttggcg gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc
     8461 aattccttta aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa
     8521 tttcgatccc tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat
     8581 ttgaactttc ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca
     8641 gaaacggtga caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat
     8701 caaggattga atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg
     8761 acagaaaatc taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca
     8821 aataatttag gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa
     8881 aaccaatggt tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt
     8941 caaacaaaac aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag
     9001 tacttggttc gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc
     9061 cagaaatacc agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata
     9121 aagaaaaagt caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata
     9181 ttgaatataa attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag
     9241 gttttcctat cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc
     9301 aactaaaagc tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga
     9361 atatgatcaa gagaaactat aaattaccag attggatcga agtcaataca gatgatctga
     9421 ctatcatgct taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata
     9481 aaatcgactt agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg
     9541 atgcttcaaa caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa
     9601 aagaaaataa aaaatcatcg gatgagtagt attagagggg gaatcataat ggaacgagca
     9661 atatttgcag gaggctgctt ctggtgtatg atccagcctt tcgacacgct tcctggtatc
     9721 catacagtga tgtcaggata tactggaggg catgtaccag atccgactta tgaacaagta
     9781 aaaagccaaa cgactggaca tacagaagca gtggagatta tctatgatcc aacattgatt
     9841 tcatatgaac aattagtaga actttactgg cagcaaactg atccaactga cgctttcgga
     9901 caattcgagg atcgcggtga caattatcgc ccggtgatct tttatttgac agaagaacag
     9961 cggatcattg ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt cgaacagcca
    10021 atcgttacaa aaatcgaacc agcccaacca ttttatctag cagaagaaga ccatcaagag
    10081 tattataaaa agcatcctga aaattttaaa agaaaccatg cacgccgggc tgcctttatc
    10141 gaagaaaatt ggaggaacga agggtgaatc gaagcttttt tcattattta atgacactgc
    10201 gaggcggtgg cacacatgat gctttatctg ttttcgctac agaagccggt aaagacatcc
    10261 aatttcctaa gcacagctct agctacgaag aaatttctaa ttatctggaa ctgaatgtcg
    10321 actatttgcc gtccatggat atctttgacg aagcatggga aaaatattta gaaaataatc
    10381 atgacatata agagatagtt ttgtacttta tgatcaaaaa agttttttgt ccaatagcct
    10441 aattgaaaga attattttta tttcagctat ctagtcgcat ttttttgata tttttcttat
    10501 aataaagaaa gaatgaaaaa aagagggatg gattgataga gattgacaaa aaaacaacgc
    10561 tatattaatc gcttctggct tgtattcaaa tacttacttg tttctgtatt ccttgcgttc
    10621 atgttgcggg gatttttatt catcccggta ccagtggaag gcaattcaat ggaaaacgtg
    10681 ttgaagcaag gtgatatggt tgtgatggaa aaattttcgg agatcagacg atttgatatt
    10741 gttgtttttc aattagcaga tggcacgatt tatatcaaac gagtaatcgg tcttccagga
    10801 gaaaatgtca gctaccaaaa tgatcaattg aagatcaatg ggaaagtagt taaggaacct
    10861 tatctaacta aaaacctgaa atcagatcat gcaaatgcat cctatacgac ggactttaca
    10921 ctacaagagc taacgggaca atccaagctt ccggaagata attattttgt acttggagat
    10981 aatcgaagag tttcaaaaga tagtcgttcg tttggcacga tcaataaaac tgatattcta
    11041 ggtaaagcaa gatttgtcta ttatccactt gatgaaataa aatggatcca gtgagaaaca
    11101 ataaagcgtg cagtctgttg agactgcacg ctttattgtt taatccaaaa tcagtttact
    11161 accttagtac gtaaaatcaa acagctcgtt taaacacttt tctagcctct aaagttttga
    11221 taaatgaata aacaaccatt atttgaatag ggatttggat catattcctg atgaatctac
    11281 ctagaaaaat tactttgaaa tctgcaccaa acaaaacaga catcagccaa gaattgataa
    11341 aaaggctgac tgggatcata atcaataatt gggcagcgat gacggaaggc aggtttatct
    11401 ttttatgata taaaaataag ccattaataa atccaccaag gaaagccgtg ataagaaaag
    11461 cagcttgaaa cgttcctgaa ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa
    11521 gcaaaccagc ccagatcggt ccatatagat aacccataag cgccatactt aaaaatccga
    11581 atgtgatttg aaaataagca ggaccaatcg ctatcttgta taagatgaca tcaagggcta
    11641 ataaaagagc agaggtcgac aacaatcttg cattcatttc gggcaatcta aaaacgtctt
    11701 ttgcaatcaa agtgttcaaa atattctctc ctttgtcagt taataataaa aggataccat
    11761 aaaatcacca atgtgtcatg acacattggt gatttacata cactggcatc taacaattaa
    11821 aaaaagggtg agaagactgc ttttataaac tgtaaaaaag agattgtacg aataaaaaaa
    11881 atagaggcgg agaatactta tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc
    11941 agattagtta tctgcttaca tcttcatcca tacgggttct aatgtatttc cgtctagatc
    12001 ttggacttct aatgaaaaca tttcttcttc aggcattccc atatctactt cgtggaaagt
    12061 accaccattt tcttttgcaa tttgagcaaa tttttttact tcatccgcat tttcaacact
    12121 aaacgaaatc aaagcaccac ttgttttttt attatcagct atttctttat cttttataaa
    12181 ttttttataa aaatcatgag agagaagcat tatccaaaaa gtgtcatccc ataccattga
    12241 accagcttct tcagtagaaa attcttcatt ttttttaaaa cccagctttt cataaaaagc
    12301 aacggaacga ttgacatcag caacaggaaa attaacaaaa accatactat tcatttgttt
    12361 cgtctccttt ctgttttgtt cgtctatcat ctcttattta ggaaacgctg tcaaaaattt
    12421 agattcatat atagaaacca aaataaaaat ttgcatcatc aaaaaagtga agatgtgata
    12481 gaatgaggac tggttcactt tcacttaact aatagatgga atttctttat aataaaaaag
    12541 tgaatttata ttgaaaaaga aaaaaacaag taaagccggg gaatattccg gtatgaaagg
    12601 aatggagaga acaatgacca tccaatggtt tccgggacat atggcaaaag cccgacgaga
    12661 agtttctgaa aaaatgaaat atgtggacat cgtatttgaa ttggtagacg cacgattacc
    12721 tttatcttct cgtaatccaa tgctagatca gatcattcag caaaaacctc gccttgtttt
    12781 attgaataaa gcggatctgg cagatccaca acagacaaag ctgtggcaag aatattttcg
    12841 taaacaaggc catcttgcat tagctatcac ggcacaagaa agtaaaggaa ttaaaggtct
    12901 agtacctgcg gctaaagaag cattgaaaga aaaacgggca agagatgctg ctcgggggat
    12961 caagcctcgc gctattcggg caatggtctt aggtattcca aatgttggga aatctacgtt
    13021 gatgaatcga ctagtaggca agaaaattgc acagacagga aataagcctg gcgtaacaaa
    13081 aggtcagcag tggcttcgtt taggtaatga gttggaattg ttagacacac cagggatttt
    13141 atggcccaaa tttgaagatg aagaaatcgg caaaaaacta gcgctgacag gcgcgatcaa
    13201 agaccagctt gtccatttag atgatttagc gatttacgga ttagactttt ttgcccgata
    13261 ttattcagga agaatcagcg atcgttatgg tttatcaatc gagcaagaag aaaaattggc
    13321 accggaatta ctgatggata ttacagcaaa acgtgggtat aaagaagact atgatcgtgg
    13381 cagcgaatta gtcgtgcatg atattcgtca aggaaaactt ggacgataca cactggatcg
    13441 ttgcgaagaa atggagcaaa aaagtgatga ctgaatccat ccaaagtatc aaagaacgat
    13501 tgaaaagcat acagagaata gatgatccta cactagaatc attaagaaat gatccacgca
    13561 aaggcgtgca gcaagcactg gcttcttttg aacgacgatt gaaaaaggaa gcattaatta
    13621 aggaaaaata tgaagaaatg ttttgctttg aaaagcaagg gtacgcacaa ggtcaccaat
    13681 ggattgccgg cattgatgaa gtaggaagag gaccattagc tggacctgtc gtggcagctg
    13741 cagttatctt gccgaaaaat gagatcatat tagggctgaa tgactccaaa caattatcag
    13801 agaaaaagcg agaacttctt tatcaacaag ttcaagaaaa agccctggca atcgggatcg
    13861 ggatcgttga tcagcagaca atcgatgatg tgaatattta tgaagcaagt aaattagcga
    13921 tgatccaagc agtagaaaaa ttgtccattg taccaagcta tttgttgatc gatgcgatga
    13981 ccttggattt gcctattcag caggaaaaaa taatcaaagg ggatgcacga tctgtgtcga
    14041 ttgctgctgc aagtatcatt gctaaagtat atcgagacca attgatgaaa gaatatgacg
    14101 tgctttatcc tggatatggt ttctcgcata atgcaggtta tggaacaaag gaacacttag
    14161 aaggacttga acgacaagga attacgccaa tccatcgtaa aagttttgca cctgtaaaaa
    14221 aatatttaca ataaaaaaca gccgctttta cgtatcttat tagaaagaga gacgaaaggc
    14281 ggttttatga tgtatcaaat agaagaaaat ttattgaaac ttgcttatac aaaaggaatc
    14341 agtagtcaag gaaaatgggc acttgctaac tggatgatgc agtatcctta taaagaaatc
    14401 ggatttgaag acatcattaa gataggtaaa ataaccaaac accgggagct gtttcgcaaa
    14461 agttggcagg aaattcacgc taactggggc aaaatccaat cgaaacagtc gtttatcact
    14521 tgctttgatc ccaaatatcc ccctcagctg cttcacttga cctatccgcc aattgtactg
    14581 ttttataatg gtgatctatc tttactttct tgtaacatgt taagcgttgt tggtggaaga
    14641 cacacttctg ctttagccaa aaaaacagtt tattatttac taaaaccagt gatagaagct
    14701 ggatatgtga tcgtcagtgg ctgcgcgaaa gggatagata catacggtca ccaagcagca
    14761 atctctcatg gtggacggac aatcgcagtc attggaacag ggatagagca agtctatcct
    14821 gctgaaaatc gtgacttgca agaggtgatt gcaaatgatc atcttctttt gagtgaatat
    14881 cctcctcaat ccggcccgaa aaagcattgt tttccaatga gaaatcggat tatcgcagct
    14941 ttgtctaaag gaacatgtgt ctttgaagcc aagaaaaaaa gcggctctct gataacagca
    15001 cagcaagcat tggatcttgg aagaacagtt ttttctgttc caggaaatat tttaacagaa
    15061 cattctaccg gatgtcatca tttgatccaa gacggtgcag tttgcacggt ttcgggtgaa
    15121 gatatcttgg cagaatttaa agaataacac catacttttc atttgctttt cttgacaaac
    15181 gttttctgaa tggatatgat tagctacgat ttgtatgaat cctcttttaa tagtaggaac
    15241 acgacaatgg aaaggagtcc attcagtaat ggcgtataaa tatttagtga ttgtagaatc
    15301 acctgcaaaa gccaagacaa ttgagaaata tcttggacga aactataaag taatggccag
    15361 tgttggccat ataagagatt taccgaaaag taaaatgggt atcgattttg aaaacaatta
    15421 tgaaccccat tatatttcta tacgcggaaa aggcgatgtc atcaaaagcc tgaaagccgc
    15481 agcaaaaaaa gctcaaaaag tttacttggc aagtgacccg gatagagaag gagaagcgat
    15541 tgcttggcat ttagcgtacc tacttgggtt ggatctgaaa gaaaaaaatc gggtggtctt
    15601 caatgaaatc acaaaagacg cagtcaaagc agcttttaag gaaccaagaa cgatcgatgt
    15661 agatttagta gatgcacagc aagctcgtcg taccttagac agaatcgttg gttattcgat
    15721 cagtcctatt ctctggcgta aggtcaagaa agggttaagt gcaggacgtg tccaatctgt
    15781 cgctttaaaa attattattg accgtgaaaa agagatccga gaatttgttc cagaagaata
    15841 ttggagcatc gacggtaatt ttaaaaaagc tcgcaagaaa ttcaaagcaa atttctgggg
    15901 aatcgacggt aagaaaaaga aattaccaga tgcacaaagt gtaaaagaag tcactgctag
    15961 aattgatgga acagacttcg aagttaaaaa agtagaaaag aaagagcgca aaagaaaccc
    16021 tgcgaatcct ttcactacaa gtagcctgca acaagaagct gcccgtaagt taaactttcg
    16081 aacaagaaaa accatgatgg tagcacagca attatatgaa ggaatcactt tgggtaaaca
    16141 aggaacagtc ggcttgatta catacatgcg tactgattct acccggattg ccgatacagc
    16201 aaaagcagag gccgcagcat ttattgaaga tacgtatgga aaagaatttt ctagacatga
    16261 tcaccgaaag atcaaaaatg ctcaaggcgc acaagatgcc catgaagctg tgcgtccaac
    16321 gagtgtgctt cgtacaccag atgaactcaa acaatattta gataaagatc aattgaaact
    16381 ttatacattg atctggtcac gttttgtggc tagtcagatg acaccagcta ttttagatac
    16441 aatgaaagtg acattagaac aaaatggtgt catcttcatt gcgaacggtt caaaaatcaa
    16501 attcaaagga ttcatgcaag tgtatgtgga aggtagagat gacggcaaag aagagaaaga
    16561 aaatattctt ccagaacttg aagaaggcga tgtagtccaa tctgttgata ttgaaccaaa
    16621 acaacatttt actcaaccac cagcacgttt tagtgaggct acgttgattc gtgcactaga
    16681 agaaaacggg gtagggcgac cttccactta tgcaccaaca cttgatacga tccaaagacg
    16741 ctactatgta aaattgacac aaaaaagatt tgagccgact gaactaggag aaattgtcaa
    16801 ttcattaatc tgtgaattct tccctcaaat cgtcgatatc catttcacag cagaaatgga
    16861 aggagatttg gataaaattg aagaaggcac ggaagcgtgg gtcaaagtgg ttgatcgttt
    16921 ctataaaccg tttgaaaaag aactgacgaa tgcggaagaa aaaatcgaaa aaattcaaat
    16981 caaagatgaa ccagcaggat tcgattgtga tgtctgtgga catccgatgg tgataaaatt
    17041 agggaaatat gggaagtttt atgcttgcag taattttcct gattgtcgga atacgaaacc
    17101 aatcgtaaaa gaaatcggtg tgacttgccc agtatgtcat gaaggacaag tcattgaacg
    17161 aaaatctaag aaaaacagac tattctatgg ttgttcccgt ttcccagaat gtgaattcac
    17221 ttcttgggat aaaccagtgg gcagaaattg tccaaaatgt gacggatatc tggtcgagaa
    17281 gaaagtcaaa ggcggtaaac aagtagtctg tgtaaatgga gattacgaag aagacatcca
    17341 aaaataatag aaaagaggtt ctttcattga aaatggtaaa tgttgttgga gcagggcttg
    17401 ccggaagtga agctgcttgg cagatcgcac aagcaggagt ccctgtccgt ttatatgaaa
    17461 tgcgtccagt caaatcgact gaggcgcatc acacaagtaa ttttgctgaa ctagtatgtt
    17521 ccaattcatt aagaggaaac agtttggcta atgcagtagg gttattgaaa gaagaaatgc
    17581 gccgattgaa ttcggtagta atcacttcgg cagatgaaac agcagtgcca gctggtggtg
    17641 ccttagctgt tgaccgcgat tctttttctg aaacaattac ccgaaaagtc aaagagcatc
    17701 cattagtaac agtgataaac gaagaaatta ctgaaatacc ggaaggaatc accgtagtag
    17761 caacaggacc tttgacttct catccattag cagaaagcat caaagcattc aatggttcag
    17821 atggttttta tttctatgat gcagctgcac caatcgtaga caaagctacg atcgatatgg
    17881 ataaagtata tttgaagtcc cgctatgaca aaggagaagc agcttacttg aactgtccga
    17941 tgacagaaga ggaattcaaa gctttccatg aggcactaat caatgccgaa gtggcagaac
    18001 ttaaatcttt tgagaaagaa aaatattttg aaggatgtat gccaattgaa gtaatggcac
    18061 agcgaggatt caaaacgatg cttttcggac ctatgaaacc agttggattg gaagatccta
    18121 aaactggcaa acgtccttat gctgttatcc agctgcgtca agacaatgct gcagcttctt
    18181 tatacaacat cgtgggcttt cagactcact tgaaatgggg cgagcaaaaa cgtgttttcc
    18241 ggatgattcc tgggttagaa aatgctgaat tcgttcgcta tggtgtgatg caccgaaaca
    18301 gctttatgaa ttcgccagaa ttgctgaaac cgacttacca atcaacgaaa cgcgaagatc
    18361 tgttctttgc aggtcagatg actggtgtag aaggctatgt tgaaagtgca gcgagcggac
    18421 tacttgctgg aatcaacgcc gcacgcttag caaaagaaat ggaaccagtc gagtttccac
    18481 aagaaacagc tattggaagt atggcttact acattactca tgcggaagga aaacatttcc
    18541 aaccgatgaa tgccaactat ggtctgtttc ctgaactgcc agagcgcatt cgagacaaaa
    18601 aagcaagata tgaagcaatt gcacaacgag ctttaaaagc taatgaggca ataaaaacag
    18661 aattagaaaa aactgcaatc ccaaatgagt aaggaaactc catgaagagg gtgagacgga
    18721 tagtctcgct ctttttcttg cgtttttctt aagaatttct caaacaaaat gtgaggaatt
    18781 gcgccaattg tgaacaaact tagaaatatt ggttaaattc tgacaattta tttgtctttt
    18841 tataaacgct gtgctaaatt attctctggg acaaaaggaa ggtgagaaaa tgacagaagc
    18901 cacgtgggaa caaaaatttt tgcgttattt gattgtcgaa agaggatatt cagaaaaaac
    18961 gaaagaagca tatgaagaag acattctcca tttcaaacat tttttggagg aatctggaga
    19021 cgctgatctg ctgaaggcgg aatattttac ggttcgtgtc tatttgagtg cattagctga
    19081 tcatcgtcct agctatagca gaaattctat cagtcgcaag atagccagcc tacgttcgtt
    19141 ttatcagtat cttctcaaag aagaagtgat aaaagaaaat ccattttctt acgttcatct
    19201 caaaaagaaa aatttgagac tgccacgttt tttttatgaa aatgaaatgc aagcactttt
    19261 tgacagcgta gcaggagata gtcctttgga tttgaggaac cgcgctttac tggaagtcct
    19321 ttacgggtca ggcatccgtt taagtgagtg tagcagtttg tcggtatcag acattgattt
    19381 tgattcagag gtcatgttga tccatggtaa agggaacaaa gagagatatg ctcctctagg
    19441 ttcttttgct caagatgctc tgcaagaata tttcagggaa ggccgccagg tattgatgac
    19501 aaaatatcac gaagagcatg atatcgtttt tgtgaatcat catggaaaac caatcacgcc
    19561 tactggaatt gaatatgtct tgaatcaaat gatcaaaaaa agcagtttgg atagtgatga
    19621 tatccatcct cacatgctgc gccatacatt tgctactcat ttattaaata atggggcaga
    19681 tttgcggaca gtccaagaat tgctaggaca tgccaattta tcgactaccc agatctatgc
    19741 ccatgtaaca aaagaaagtt tacaaaaaaa ctatcgttct ttccatccaa gagcgtaaaa
    19801 ataaggaaaa gaacaaattt aggaggaaac aaacatggtc gaatcaccat ttcattcaac
    19861 cactatctgt gccgtcgaaa aagacgggaa atttgctatg gctggagatg gccaagtaac
    19921 aatgggtgaa tctgttgtca tgaaaggcac tgcaaagaaa gtacgacgta tctacaatgg
    19981 agaagttgtt gtaggtttcg ccggaagtgt tgcagatgcg tttactttag aagaaaaatt
    20041 tgaaggcaaa ctaaacgaat ataacgggaa tctacaacgc gcagctgtcg aattagctca
    20101 agaatggcgg acacagcaat ctatgcaaaa acttgaagcg atgctgatcg taatgaatga
    20161 taaagaaatg cttcttgttt caggtacagg agaagttatc acaccagatg atggtatctt
    20221 agctatcgga tcaggaggga attttgcgtt ggctgcagcc cgagcaatga aaaactatgc
    20281 tcaaaacgac atgagtgcga aagaaattgc agaaaatgcg ttaaatattg ccgcagatat
    20341 ctgtatcttt actaatcata atattatcgt agaagaactg taagaggtga gtagatatgt
    20401 atgaattaaa acaaacacca agacaaatcg taagtgaatt agatgaatat atcgtaggac
    20461 agcacgcagc aaaaaaatct gttgccgttg ctttgcgtaa ccgctatcgc cgtttgcagt
    20521 tagatgaaaa aatgcaacaa gatattacac caaaaaatat gctgatgatc ggacctacag
    20581 gggtcggtaa aacagaaatc gcacgacgtt tggcaaaaat cgtcaatgca ccatttgtca
    20641 aagtcgaagc aacaaaattt actgaagtcg gttatgtcgg aagagatgta gaatcaatgg
    20701 ttcgggattt agtagaaaat gcgatccaaa tcgtagaaaa agagcagtac agccgtgtat
    20761 acacaagtgc taaaaaaaat gcagaaaaac gtttggtaaa aatcttggta cctggtatca
    20821 aaaaagaaca gaaacaatcc actaatcaat ttgaacaaat gatgaatctt tttaatgcta
    20881 gtcagcaaaa taccgctgaa ccgcaagaag aagtgacaga agatattcga atcaatcgcc
    20941 gtacagtctt ggaacaacta caaaagggtt tactggatag tcgtgaagta accattgaag
    21001 ttgaagacca aaagaaacct gcaccaatga tgaacaatgg attggagcaa atggggatcg
    21061 acctaaatga aactctaggt gctttgacac cgaagaaaaa agtggaacgg acggtaacag
    21121 tcaaagaagc tttagagtta ttgatcaagg aagaatcagc aaaattagtc aaggatgctg
    21181 atatccatag tgaggcaatc aaattagcgg aagaaagtgg aattattttt atcgatgaaa
    21241 tcgataagat cacttctaaa tcacagcaat ctggagaagt atccagagaa ggcgtccaaa
    21301 gagacatatt gccaattgtc gaaggttcac aagtaaacac aaaatacggg atcatccaaa
    21361 cagatcacgt cctattcatt gcttcaggag ctttccattt gtctaaacca agtgatctaa
    21421 ttccagaact tcaaggacgc ttcccgatac gggtagaatt agatgatttg acagctgaag
    21481 attttgtgaa gattctgacc gaaccaaata atgctttagt aaaacaatac gtcgcgttga
    21541 tcggtacaga aaatgtgtca gttacgttta caaaagaagc aattgaacgg atcgctcaaa
    21601 tcgctttcga tgtcaaccgt gacacagata atatcggcgc acgcagactg cataccattt
    21661 tggaaaaact actagaagat ctattatttg aagcaccaga tatgcaaatg ggagaaataa
    21721 cgatcacaga agcatatgtg gatgacaaac ttggctcaat tgcggcaaat gaagatttaa
    21781 gtcgctatat tttataaaaa atcggaggac gacaatgaca gacttgctaa cgaaaacccg
    21841 caagataaac acacttttgc agcaaaagaa tacttttgac atgcaagcag atttgcctta
    21901 cgataaaatg gctgtcacac taggtggtat tcttgacagc aacacctata tcataggaag
    21961 tgacggagta ttactaggtt ataatgaacg tcacgatgtg aacaatgaac gaatcaaaac
    22021 aatgtttgtt gagaaacaat ttcctgaatc ttatactgat acagtggcac agttgatgaa
    22081 aactgaagct aatatcccaa ttactagtga tattactgca tttccggtag agtcaagaga
    22141 gaaatatcca tttggtctga cgactgtcgt tccgatcttt ggagctggcg aacgtttggg
    22201 tacgattatc ttatctcgta tggaacaatc gtttgcagat gacgatctag tcttagcaga
    22261 atatggagct acggttgtcg gtatgcagat cctttatcag aagtctcgaa gcatcgaagc
    22321 agatgtacgg agtgcaacgg ctgtccaaat ggcaatcaac acattatctt atagcgaatt
    22381 gaaggctgta caagctatct ttgaagcatt ggatggagat gaaggtcgtc tgactgcttc
    22441 taatattgcc gatagtatcg gcatcactcg ttctgtgatc gtcaatgctt tacgaaaatt
    22501 ggaatcagct ggaatcattg aatctcgttc gttaggcatg aagggaactt atttaaaagt
    22561 attgaattcc cgttttaaag acgagttagc aaaacacagt tattagaagt aaggggactg
    22621 gggcagatag aaatgtttgt tccagccttc ccattttaaa aagtaagtcg aagttggagg
    22681 aatggcaatg actgttatta ttcaaaatga ccagctgatc gccgaaatcg ctgaacacgg
    22741 tgcggaattg atcagtctga aatcaaaaga acatggttta gagtatatct ggcaaggtga
    22801 tccagcgtat tggggcagac atgcaccggt tttattccct tttgttggac gcttaaaaga
    22861 tgatcagtat gtttatcaag ggaaaacata tcaaatgggg caacacggat ttgctcggga
    22921 tatggatttt gaagtcattg aacatggaca agaaatggct agtttcatct taaggagtac
    22981 gccggagaca ttagaaaagt atccatttga ttttgaatta gtgatcagct atgaattagg
    23041 aggagacggc atcacggttc attatcaagt ggcaaatacc ggtgaaaaag aaatgttctt
    23101 ttctatcggt ggacatcctg ctttcaatgt acctcttgaa gaaggattgt catttgaaga
    23161 tttctatctg gcattttctc cattgaaatc aagattacgc ctaccattga aaggcccatt
    23221 catcgatatg gatcaaaaga cgttaggtca gacaaatacg aatctggctt tgaatcgtga
    23281 actttttgag caagatgcct tggtttttga aacaaggggt ctaaatgcgt acgccattcg
    23341 aagtgaaaaa tcttcccata gcgtaacttt aagctataaa gatatgcctt atgtagggat
    23401 ctggtctcct tatccaaaag aagcgccttt cgtttgtatc gaaccgtggt gcggaatcgc
    23461 tgacacggtg aacagtagtg gaaacttgat ggaaaaagta gggatcaatc aactaggtgc
    23521 ccatgaaatc ttcaagacaa aatattccat cacagtgaag taagaggttg tgaccgagaa
    23581 gttagcaggg aacaccgttt accaaggtgt gtaaaaaagc tatcctgtaa gtaataaggg
    23641 agtgtgacat cgattt
//
LOCUS       DAGCXG010000033        22796 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGCXG010000033 DAGCXG010000000
VERSION     DAGCXG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.034"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     regulatory      95..174
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            172..1365
                     /locus_tag="MB022_001340"
     CDS             172..1365
                     /locus_tag="MB022_001340"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBT4466348.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1495..2967
                     /locus_tag="MB022_001341"
     CDS             1495..2967
                     /locus_tag="MB022_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4466349.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3050..3838
                     /locus_tag="MB022_001342"
     CDS             3050..3838
                     /locus_tag="MB022_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBT4466350.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3905..4573)
                     /locus_tag="MB022_001343"
     CDS             complement(3905..4573)
                     /locus_tag="MB022_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBT4466351.1"
                     /translation="MEIRLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4688..6400
                     /locus_tag="MB022_001344"
     CDS             4688..6400
                     /locus_tag="MB022_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466352.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6403..8172
                     /locus_tag="MB022_001345"
     CDS             6403..8172
                     /locus_tag="MB022_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466353.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8377..8943)
                     /locus_tag="MB022_001346"
     CDS             complement(8377..8943)
                     /locus_tag="MB022_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4466354.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(8943..9899)
                     /locus_tag="MB022_001347"
     CDS             complement(8943..9899)
                     /locus_tag="MB022_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBT4466355.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9899..10555)
                     /locus_tag="MB022_001348"
     CDS             complement(9899..10555)
                     /locus_tag="MB022_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4466356.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            10925..12943
                     /locus_tag="MB022_001349"
     CDS             10925..12943
                     /locus_tag="MB022_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4466357.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13013..13219
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13370..15784
                     /locus_tag="MB022_001350"
     CDS             13370..15784
                     /locus_tag="MB022_001350"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBT4466358.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16132..17685
                     /locus_tag="MB022_001351"
     CDS             16132..17685
                     /locus_tag="MB022_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4466359.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18066..19850)
                     /locus_tag="MB022_001352"
     CDS             complement(18066..19850)
                     /locus_tag="MB022_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466360.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19865..21586)
                     /locus_tag="MB022_001353"
     CDS             complement(19865..21586)
                     /locus_tag="MB022_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466361.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21686..22447
                     /locus_tag="MB022_001354"
     CDS             21686..22447
                     /locus_tag="MB022_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4466362.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKA
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7255 a         4079 c         4715 g         6747 t
ORIGIN      
        1 ttttttggac agttctaatg taaaatattt ttatattttt taagcaaaaa aatgaaaaaa
       61 tacgagcaga aaactgtttt atctgctata cttggttcgt tgcaagttcc cgaaaggatc
      121 cgcaggacgg agatgccttg taaccgaatt taactagggg gaatcattat aatggtagaa
      181 agacacttat ttacatcaga atctgtttct gaaggacatc cagataaaat tgctgaccaa
      241 atcagtgatg caatcttgga tgcaatttta aaacaagatc caacagcacg agtagcgtgt
      301 gaaacgtctg taacaactgg tcttgtttta gtatttggag aaatatctac gacggcatat
      361 gtagatattc aaaaagtcgt acgggaaaca ataaaagaaa ttggttatac acgtgcaaaa
      421 ttcggatttg atggagatac agcagctgta ttggttgcga ttgatgaaca atctcctgat
      481 attgctcaag gagttgatga agcacttgag atccgggatg aagataaaaa agacgtatta
      541 gatgagattg gtgctggtga ccaaggttta atgtttgggt ttgctgtaga tgaaacacca
      601 gaattgatgc ctttaccaat cgctttgagt catcgtctgg tacgacgctt ggcagatttg
      661 cgtaaatcaa atgaattaac gtatttacgt ccagatgcaa aatctcaagt aacggttgaa
      721 tatgatgatc aaggacaacc ggaacgcgta gatacaatcg ttatttcaac acagcatgat
      781 gatgcagtag ataatgaaac aattcgtcat gatgtcatcg aaaaagtagt gaaggaagtt
      841 attccagctg aattattaga tgatcaaacg aaatattata tcaatccgac tggccgattt
      901 gtcattggtg gtcctcaagg ggatgccgga ttaacaggaa gaaaaatcat tgttgatacg
      961 tatggcggtt atgctcgtca tggtggcggt gctttttctg gtaaagatgc gacaaaagtt
     1021 gaccgttctg ccagctatgc tgcacgttat attgctaaaa acattgttgc agcagggctt
     1081 gctcgcaaag cagaagtaca gctagcttat gcgattggtg ttgctcaacc tgtttcgatc
     1141 tcaatcaata ctttcggtac tggaacggtt ccggaagaag aactgattgc tgcagtgaga
     1201 gaaaacttcg atcttcggcc agcaggaatc atcgagatgc ttgatttgcg tcgtccgatt
     1261 tacaaacaaa cagctgctta cggtcatttt ggtcgtacag atgtagattt gccttgggaa
     1321 catacggaca aagtagatgc gttgaaagca agtttagcaa aataagttat attcagctgg
     1381 aacgatcgcc aagtggtggt tgttccagct ttttttatgg cagttaacct cttgaaaaag
     1441 atagtaatta tttatctatt tttagatcct gtcataatga aagggaggaa taagatgaaa
     1501 aaagaaacaa atgttacttt ggtcacagtg agtattttca ttgcaacgtt catgacggct
     1561 attgaaggta caatcgtaac aaccgctatg ccaacgattg tcggttcatt acatggaatg
     1621 gaaatcatga actgggtatt ttcgatttat ttgctgacaa acgctatgct tactccgatt
     1681 tacggaaaac ttgcggataa agtgggacgg aaaccaatct ttatttttgg gacattattg
     1741 tttattatcg gttcgttatt ttgcggttta tctcaaagca tgattgggtt gattgctgct
     1801 cgtgcgattc aagggatggg tgctggggca atgatgccag tagctttgac gatcatcggc
     1861 gatctttatt ctattgaaaa gcgagcaaaa gttttaggtt tgaatagttc agcctggggg
     1921 attgccagtg tttttggacc gcttgcggga ggattcatcg ttgaaacgat cagctggcac
     1981 tggatctttt ttatcaatgt gccgatcgga attctattaa ttattttgat ttggctatat
     2041 ttaaatgaac caaaaatcat acatgagtct aaaccaatgg atatttttgg tagttttagt
     2101 ttgatggcca tgcttgtctc attgctgtta gggttccaat tattaggaga cgaaggaatg
     2161 agtccgcttg tttctgcttt gctgctaggt agtatcgtaa tgttaggtat ttttgttttt
     2221 gccgaaaaaa gagctaaaga tcctgtcatc atgatgcatt tattcaaaaa tccactattt
     2281 gttgtggtca acttggctgc agcattggct agtggttttt tgatgggaat cgatgtttat
     2341 atccctatgt ggatgcaagg tgtattaggg ctgaatgcag caattggtgg gctggtatta
     2401 gcaccattat cactagtctg gatggtcggt tctttccttg caagcaagtg gatgactcaa
     2461 atgaaaactc attgggtctt gagaattgga ctactaatcg cagctattgg gggcgcttgg
     2521 ttgtatctta tgcctttcag aagtaactat ttatggtttt ttgctatatc aacggtcatc
     2581 ggtataggct taggtgtgac catgactacc tcgactgtca gtgcacaaaa tagtgtatct
     2641 cgtgaccagc ttggtgtagc aacttctttt aatacgttag ttcgaacaat tggtcaaaca
     2701 gtcatggttt ctatttttgg tttattgata aatggggtaa ctaaacgaga acttgcagca
     2761 gcaaatttaa caaatgatgc agatatcatg aataaactgg tgaatccaca aacggctaaa
     2821 tatattgatg cggatatttt gttaccatta agaaaaattc tttatgatgg gttacattcc
     2881 gtttatctag ttggcttgtt actcgtgatc attgctttgg tcttaacgct tttcgttcat
     2941 gaaaaaagtg gtaaaataaa acagtagttt cggttaagat atgtacgtag ccgaatcatt
     3001 caaatttaaa gttattcaaa aataacttct tactaaggaa ggcgaaaata tggcatttat
     3061 tcaagcaaat atttattcga atgtgttaga aatggaagtc aatctaaatg tgatactacc
     3121 gcaaacaaca aaaaaggtga ttggcaccag ttcagagcaa gagacaacgg atatacctgt
     3181 tttatattta cttcatggaa tgggtggcaa tcatagtgta tgggaaagac gtacgtcgat
     3241 tgaacgttat gtagcaaact acggattagc tgtcatcatg ccatctactg atcttggctg
     3301 gtacacagat acagtttatg acatgaagta ttggacattc attgcagaag agctacctga
     3361 gatttgtcat gaattattcc cgcaattaac aagaaaaaga gaaaaaacat atgccgctgg
     3421 attatcgatg ggcggatatg gcgcgttaaa actaggcctt gcaaaatcag aaaatttcgc
     3481 agcagtaatc tctctttcag gcgctgtttc tgtaggaaat cgaatggatg atcttttgat
     3541 ggttcgtaaa gcatcttttt gggaagggat ctttggacca ttggatcaaa tcgaaggttc
     3601 aaaaaatgat ccgcaatact tattgaaaga actagtaaaa agcggaaaac cagttccaag
     3661 attttatatg gcttgtgggg aatctgattt tctttatcag gcaaatcagg aaatggtatc
     3721 tgctatgcag gaacaaggct tggcggtcac ctttgaacaa ggacctggcg atcataactg
     3781 gatcttctgg gaccaatgga ttcaacgtgc attggcgtgg ctaatggaag aaaaataaaa
     3841 aaagagtctt aaaaagatag ctcctataga gatcacaaac atagtgatct ctataggagc
     3901 ttttttactg ctcgttttct cttcttttat tttgttggca taagccttca atataggttt
     3961 gtgcactcgt tagaaagagt gctttttcat tcaattctgc atgggactgc atactttctt
     4021 taaatccctc taattgattt tcaactacat aattacgcat aaatagaact gtttctttca
     4081 aaaattcatt tccgtccata attttttgtc gaagctgctg ttcatgctgc tgatccatta
     4141 actgtccaat caataaattg tggagagagg caatggcctg atgactttgg gtgactgaaa
     4201 atccttcacg aacaagtaat tggatcattt gctccaaatg ttctaatcga ctcggaaaag
     4261 cgggcacagt attgatttct aattctgccc cacaagggaa acgtgtataa aggtcatagt
     4321 aattttccat atactgcagc aactgttcat tccaaggcaa agttggatct ggaagcgcta
     4381 attcttcttc cattgtttca gccattattt gcaagagctg ttgtttgttt ttgaaatacc
     4441 aatataaagc aggtgcttga acatgaattc tgtttgcgat gtttcgcatg gaaagctgcg
     4501 aaagagcttg tgtttcagcc aggatatcaa aggcggcctg aatgatgatt gcttgagaaa
     4561 gccttatttc cattttatca acctcgtgta ttgcattatc tttttaaaca tggtaaattg
     4621 ttagtcggtc taattttaca gtgttaaatt agtcctgtca atttaaacaa aggggtgtaa
     4681 tcaacgaatg aaattaatct tacgacatgc gaaaaaatat aaagtagctg tttttatttc
     4741 cttgctgtcc gtagcagtca tggttgcagc agcattatgg caacctaagt tactacagca
     4801 agtattggaa gctattatta cagaagataa tgatgaaatg aaaacaattg gtatttacct
     4861 gatcagtctg gctttgctag gattagccgc aggtgtaacg aacacgattt tctctgcaaa
     4921 agtagctcaa ggcgtaagtg cagatatccg agaagaagca tttcgaaaga tccagacatt
     4981 ttcatttgga aatatcgaac agttctctgc tggaaatctt gttgttcgtc taacaaatga
     5041 tatcacgcaa atccaaaatt tagtgatgat ttctttgcaa tcactttttc gaattccatt
     5101 cttatttatt ggtgccttca ttttagctat gatcacgatg ccgcaattat ggtggatcat
     5161 cgtagcgctt atcgttacgg tatttctgat tacggcactt tcatttacaa ggatgggcaa
     5221 gcacttcatg atcattcaaa aactgattga tcgtgtgaat agcattgcaa aagaaaactt
     5281 actagggatt cgtgtagtga aatcttttgt acaagaagaa aaccaattga agtcattcag
     5341 caaagtaagt gaggacttaa caaaacacaa catcatagtc ggtacattat tttctgtgat
     5401 gatcccgtcc ttcatgctgg ctgccaattt ggctgtggtt ggggcgatct tttttgtgag
     5461 cgatcttgtt aaagatgatc ctacgttaat tggtggaatt gcttcattca tgaactactt
     5521 gatgcagatt atgatggcaa tcattattgg cggaatgatg atgatgatga cttcaagagc
     5581 agccgtatcg ctgaagcgga ttgcggaaat tttagatgct gaaccagatc ttacttattt
     5641 agacgttcca gaacaagagt tgacgggttc tgtcactttt gaccatgttt cttttcgtta
     5701 tcctggtgag gatacagata cgcttaaaga tatcagcttt tctatcaaac ctggagaaat
     5761 ggttggtatc gtcggggcga ctggtgccgg gaagtcaaca ctagcacaat tgatccctcg
     5821 tctatttgat ccgacagaag gcaaagttga agtaggtggt gtagatcttc gtcaagtaaa
     5881 tgaaaagagt ttaagacaaa cggtttcctt tgttttgcaa aaagcaattt tattctcagg
     5941 aacgattgct caaaacttgc gccaagggaa aaagaatgca acagaaaaag agatggaaca
     6001 tgcttcttct attgctcaag caaaagaatt catcgaaaag ttatctgatc gttacgaagc
     6061 accaatagaa gaacgtagca gtaacttttc tggtggtcaa aagcaacgtt tatccattac
     6121 tcgtggcgtt atcggtaatc caaagatctt gatcctagat gacagtacga gtgcactgga
     6181 tgctcgctct gaaaagctgg tccgagaagc actcgatcga gatttgaagg gaacaacgac
     6241 gattgtcatc gcacaaaaaa ttgcttctgt tgtcaaagca gaccgcatct tagtattgga
     6301 tgaaggcaga ttagtcggcg aaggaaccca tgaggaattg gtagctggaa accgtgtcta
     6361 tcaggaaatc tttgaaactc aaaaaggaac ggaggagtaa gtatgactga tctaataaaa
     6421 gcaagtaaat ttttctatca ctatttgaag cggtacaaac tctccttttt ctttatcttt
     6481 atcacagtga tcattgctac ctatctacaa gtaaaggcac ctcaatacat cggggaagct
     6541 tttcaagaat tagctaatta cgtaggaggc ttgatgcaag gcgtagacga taaaagcaaa
     6601 tttgtttctg taatatggaa actcttgctg ttttatgtac tagctagtgc agccaacttt
     6661 atctatagta ttttgttcac acaagtagta gggaagtcaa cgaaccgaat gcgtatcggt
     6721 ctattcaaca aattggaaaa attgacgatc cgtttctttg attctcatca agacggtgaa
     6781 attttaagtc gttttaccag tgacttagat aatattcaaa atagtttgaa ccaagctttg
     6841 cttcaagtca tcacgaatgc tgttttactt gtcgggatct tgatcatgat gttccgccaa
     6901 aatgtagagt tagcttgggc tacaattgct tccactccag tagccgtttt gatagctgtc
     6961 ttgattatcc gcaaagcacg gaaatatgtg gatcttcagc aagatgaagt ggggaaacta
     7021 aatggttata tggatgaaaa aatcagcgga caacgtgtca tcatcacaaa tggcttacaa
     7081 gaagaaacga ttgaaggatt cttagagcat aatcaaagtg ttcgcgaagc aaccttcaaa
     7141 ggacaagctt attctggtct actatttccg atgatgcaag gaatgtcact ggtcaataca
     7201 gcgatagtta tctttttcgg cggctggtta gcattaaatg gtgatttgga acgttcggtg
     7261 gcattaggct tagttgtgac attcgtccag tattcacaac aatattacca accactgatg
     7321 cagatttcat ctggatacag catgatccaa ttagcgatta caggggcaag acgattgaat
     7381 gaaatatttg atgaaaaaga tgaagtcaaa ccagaaaact gtaaagcatt tgatggtgtt
     7441 gaaaaaggtc ttgcgttgca gcacgtagat tttggttatt ctccagaaaa actagtcttg
     7501 aaagatgtct cgatcaatgt ggacaaagga gaaatggttg ctcttgttgg tccaacagga
     7561 tcggggaaaa cgacgatcat gaatctgcta aaccgttttt acgatgtcaa tgatggttca
     7621 gtagtcatcg atggaataga tatccgagat tatgatcttg atgcattacg tagacatgta
     7681 ggaatcgttt tgcaagaatc tgtattgttc tccggaacga tcaaagacaa tatcacattc
     7741 ggcaaaccgg acgcttcaga tgaagaagtc atcaatgcag caaaacaagc gaatattcat
     7801 gacttcatca tgacgttaga aaaaggttat gatacagaga tatccgaaga aaataactta
     7861 ttcagtacag gtcaaaagca gttgatcagt attgctcgaa cgatcatcac aaatccatct
     7921 ttattgattt tggatgaagc aacaagtaat gtagacacgg taacagaagc aaggatccaa
     7981 aaagcaatgg atgaagcaat caaaggaaga accagctttg tgattgctca ccggctgaaa
     8041 acaatcttga atgcagatag aatcgttgtc ttgcgtgatg gggaagtcat tgaagaaggc
     8101 agtcatcacg agttattagc agaaaatgga ttttatgcgg aactttatca taatcaattt
     8161 gtatttgaat aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
     8221 caaaaacagc ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt
     8281 gagcaccgtg aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa
     8341 acgcaaaaca tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata
     8401 taaaataggc ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc
     8461 ctgtggcagt ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg
     8521 accatagtag acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc
     8581 catagctgtg cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata
     8641 ccctagattg aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt
     8701 ttcgtggcct tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc
     8761 acgagtagca tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc
     8821 gatgtgttct gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg
     8881 atcccctggt tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa
     8941 catcagacgc tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga
     9001 agttcttcat caattgcatt caagacttcc cattttttca agctccacat tggtccaatc
     9061 aaggaatccc acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt
     9121 tccagctggt cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct
     9181 tcatgatagt cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt
     9241 ccttggatat ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt
     9301 tcgcctggta atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta
     9361 gccacgccat ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct
     9421 tcaaaagttg tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca
     9481 aggtaatcaa ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg
     9541 acacctggta aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg
     9601 gtgtttgtaa aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc
     9661 atcatgtcga tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg
     9721 tctcccgaac cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga
     9781 ttcggacagt caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt
     9841 cgtaaagcgt aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt
     9901 aaaagtcctt ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat
     9961 aagtcaggca cagccaagaa agactgagta agtaaccgcc gatgatatca gtaggaaaat
    10021 gaacgcccag ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt
    10081 gaagcaatgt gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac
    10141 cgtaaagaat catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta
    10201 ctaagtgcgc caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga
    10261 ttccagcaat tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat
    10321 accacatcca taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt
    10381 tagtcataaa gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa
    10441 accactgatc gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga
    10501 aaaacaggac aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat
    10561 tcctcctttg aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag
    10621 agataaaacc aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact
    10681 gtaatcggaa agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt
    10741 ttttcgaaat ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag
    10801 ttgttcatta cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt
    10861 ttaataaata ttaagagaag ttataattat taataataga taaattgaag ttggaggata
    10921 gaagatggaa aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg
    10981 aaaagcaaaa cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc
    11041 cccattagta cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag
    11101 aatcaataca gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc
    11161 caacgatcta tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag
    11221 tagtgcgtta tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg
    11281 acaaaccgtc tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga
    11341 gcctttccga caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag
    11401 aaatacttca tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc
    11461 ctatacagat gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg
    11521 caaattaaat aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg
    11581 aaacgcaggc ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg
    11641 cggttcaagc aattctggta atacaaactc aggttcagca acttcgacca cttatactgt
    11701 ccaatctggt gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt
    11761 gatccaatgg aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa
    11821 aggtagccag gaaaatggct cttcgacgaa taattccggt aacaatacca attcttcagg
    11881 aaatgctggt acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg
    11941 tgattctgtt tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg
    12001 gaacaatatc aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg
    12061 ttcttctaat gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc
    12121 gtccagctca agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga
    12181 ctctgtttgg agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa
    12241 caatatccaa aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc
    12301 aacaaacagc acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt
    12361 taatactggc agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg
    12421 gagcgtggca aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa
    12481 aaacaacttc atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac
    12541 aaatacaggt tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac
    12601 aagtacatca tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag
    12661 cgtagcgaac aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa
    12721 taatttcatc tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa
    12781 ccaacaatca gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag
    12841 tgttgctgac agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa
    12901 caactttatt tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa
    12961 ataacgattg aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac
    13021 ttgaaaagga agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg
    13081 cacggatgct tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg
    13141 gcggtgacgg ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg
    13201 cgtagattac gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg
    13261 aagtttgaga aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt
    13321 tatatttata atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa
    13381 tcataaagaa attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac
    13441 ccatgatgat cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg
    13501 tcaagggcta catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt
    13561 gaaaagaagt caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc
    13621 tgctgaacaa tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat
    13681 cgaaacattc cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt
    13741 gaatacaact gatccggagt attataaatg gacacaatgg atctttacga agctttatga
    13801 aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt
    13861 tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag
    13921 aaaaccaatg cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga
    13981 tctagaatta gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg
    14041 ttcagtagga gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt
    14101 tacgacacga ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga
    14161 tcttgttaga gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga
    14221 gacagctaaa aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt
    14281 tactggtgct tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga
    14341 ttatgtatta tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg
    14401 tgactttgaa ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga
    14461 tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg
    14521 catgaacaaa caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg
    14581 gaaaaaagaa gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg
    14641 tgaaccaatc ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga
    14701 gctaccatta cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc
    14761 attagcaaat atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg
    14821 tcgcgaaacg aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat
    14881 cgaccctcat aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt
    14941 tgacatctac attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg
    15001 gcataaattc ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata
    15061 caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt
    15121 agtcaatcct gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat
    15181 gttcatgggg ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg
    15241 taaattcttg gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg
    15301 tatcactaca ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt
    15361 gacagaagac tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat
    15421 caatgaagcc tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact
    15481 actcgcaccg attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg
    15541 tggtatttct tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt
    15601 agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt
    15661 aggaaaagat gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga
    15721 aggcaaaaca gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa
    15781 ttaattggct ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa
    15841 tgaacttcat tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa
    15901 aggttttgat tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt
    15961 aagagatctc tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa
    16021 actacctgaa attgagagta cataccaaat tcataaattt tttattagtg ggactagtga
    16081 ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa
    16141 gaactattta ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa
    16201 agagcgaaaa gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt
    16261 ttcggagctg tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg
    16321 atgcaaaaat taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt
    16381 gtgcttattg tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc
    16441 gatcaatggc ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat
    16501 gatgactatc tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt
    16561 gattgccagt tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa
    16621 tcaaaattcg gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa
    16681 aaaagccagt cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt
    16741 tttttcctca ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg
    16801 tcttctcata taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata
    16861 caaaagacaa atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa
    16921 aagataaatg atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt
    16981 ggtgctaatt gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc
    17041 ctgacaacgg ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc
    17101 gatttctcat ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat
    17161 aatggtaaaa catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga
    17221 aaagcggtat tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct
    17281 gcagaaactt tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga
    17341 agtacaatga tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt
    17401 gtttcgacac gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca
    17461 gttctgtcga ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat
    17521 gaagttactc aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga
    17581 ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca
    17641 tggcaattcg ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg
    17701 agcctgacgt tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg
    17761 ttgaatagtg taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta
    17821 tagcttctcg tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg
    17881 tttattcgta aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa
    17941 ggagctatcc aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta
    18001 catttaaaaa agctatcgat catggactaa tcatacatga tcgatagctt tttagataat
    18061 gtttgttacg ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg
    18121 agtagttcag aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg
    18181 tctgcatttt caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc
    18241 acagcttcca ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct
    18301 tcatctaaaa taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt
    18361 tgctgtcctt gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc
    18421 agtgttcgaa taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt
    18481 gtggcatcca ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta
    18541 ttttgtaaaa ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca
    18601 agaccgtcga attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact
    18661 aaagtcgttt ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact
    18721 gaaaaatcga cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg
    18781 aaagctattt ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca
    18841 tctgtttcat ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag
    18901 ttgatcacat aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa
    18961 gcttgtaaga agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca
    19021 cttatgatga aggcgagttg attaatgaaa cgaatggccg gatagatagc aaagttcata
    19081 aattgagcac gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct
    19141 gcatgagctt gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt
    19201 cgattattta aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc
    19261 gcaatcttag ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc
    19321 ttgctttcga tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa
    19381 aattgattga tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga
    19441 ctaatgatgt cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt
    19501 tttgtgattt cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt
    19561 tcctgaataa atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg
    19621 aacaacaatg cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt
    19681 tctgataaga gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg
    19741 ttcccgaata aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt
    19801 ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt
    19861 ctttctatat tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt
    19921 cttcatgagt accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt
    19981 gttggattgt actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat
    20041 gaagggctgc tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga
    20101 atacatagat ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc
    20161 cccctgaata attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt
    20221 ccactagttc ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat
    20281 attcatttcc catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag
    20341 ggacataact gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac
    20401 caagggttac ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac
    20461 tttttccaga tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat
    20521 caatatcttc tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag
    20581 cagctgcatt tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa
    20641 tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga
    20701 taatgaccaa taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga
    20761 ctacagcagt cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc
    20821 acaaaataga agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa
    20881 atattcggtt cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct
    20941 tttcttcata tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc
    21001 tcaatgtgat ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc
    21061 gtttcatcag tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg
    21121 ttagtaatag agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg
    21181 gtaagatcat ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca
    21241 ttcttgtcaa aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga
    21301 atttacggaa atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa
    21361 agtaactaga ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc
    21421 caattttttt tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta
    21481 ctaattttgg tacacctaat gtaccggcga tttgtaaaca gatcatcaca aaggtaagga
    21541 gagcgagtcc tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt
    21601 tttcctgata cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa
    21661 taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga
    21721 actatttgat ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc
    21781 aagtagacaa gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta
    21841 tgatatcgtt ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg
    21901 aaaaacattg acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga
    21961 actggtggtg atgaaacaaa aacaaaaagc gataagggag cgaaaaaaac agttgaaatt
    22021 gatgatcgat acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat
    22081 ctcaacggag tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt
    22141 tgggatgatg agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc
    22201 tagtgaagta catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta
    22261 tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat
    22321 cagagaaaga atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt
    22381 gacgaatacg gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa
    22441 aaaataggaa atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa
    22501 aaattttctg gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa
    22561 ttttagtttt ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata
    22621 tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt
    22681 ataattcatg gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt
    22741 gtatcagatg agaatgataa attaaatagg tgcataaaag cattacactt aaaata
//
LOCUS       DAGCXG010000034        22009 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGCXG010000034 DAGCXG010000000
VERSION     DAGCXG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.035"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            104..910
                     /locus_tag="MB022_001355"
     CDS             104..910
                     /locus_tag="MB022_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBT4466363.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            928..1755
                     /gene="vanW"
                     /locus_tag="MB022_001356"
     CDS             928..1755
                     /gene="vanW"
                     /locus_tag="MB022_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HBT4466364.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1752..2723
                     /locus_tag="MB022_001357"
     CDS             1752..2723
                     /locus_tag="MB022_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4466365.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2716..3744
                     /gene="vanB"
                     /locus_tag="MB022_001358"
     CDS             2716..3744
                     /gene="vanB"
                     /locus_tag="MB022_001358"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4466366.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            3750..4358
                     /locus_tag="MB022_001359"
     CDS             3750..4358
                     /locus_tag="MB022_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4466367.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4424..4597)
                     /locus_tag="MB022_001360"
     CDS             complement(4424..4597)
                     /locus_tag="MB022_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466368.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            4945..5376
                     /locus_tag="MB022_001361"
     CDS             4945..5376
                     /locus_tag="MB022_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4466369.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            5383..5616
                     /locus_tag="MB022_001362"
     CDS             5383..5616
                     /locus_tag="MB022_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466370.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            6040..6240
                     /locus_tag="MB022_001363"
     CDS             6040..6240
                     /locus_tag="MB022_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBT4466371.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            6324..7517
                     /locus_tag="MB022_001364"
     CDS             6324..7517
                     /locus_tag="MB022_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4466372.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            7695..7925
                     /locus_tag="MB022_001365"
                     /pseudo
     CDS             7695..7925
                     /locus_tag="MB022_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            8053..8736
                     /locus_tag="MB022_001366"
     CDS             8053..8736
                     /locus_tag="MB022_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBT4466373.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            9045..9118
                     /locus_tag="MB022_001367"
     tRNA            9045..9118
                     /locus_tag="MB022_001367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9079..9081,aa:Arg,seq:tct)
     gene            9277..9636
                     /locus_tag="MB022_001368"
     CDS             9277..9636
                     /locus_tag="MB022_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBT4466374.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            9759..11390
                     /locus_tag="MB022_001369"
     CDS             9759..11390
                     /locus_tag="MB022_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBT4466375.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(11452..12093)
                     /locus_tag="MB022_001370"
     CDS             complement(11452..12093)
                     /locus_tag="MB022_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4466376.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    12279..12355
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            12375..12683
                     /gene="rplU"
                     /locus_tag="MB022_001371"
     CDS             12375..12683
                     /gene="rplU"
                     /locus_tag="MB022_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBT4466377.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            12710..13054
                     /locus_tag="MB022_001372"
     CDS             12710..13054
                     /locus_tag="MB022_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBT4466378.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            13067..13360
                     /gene="rpmA"
                     /locus_tag="MB022_001373"
     CDS             13067..13360
                     /gene="rpmA"
                     /locus_tag="MB022_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBT4466379.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(13453..14601)
                     /locus_tag="MB022_001374"
     CDS             complement(13453..14601)
                     /locus_tag="MB022_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4466380.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(14613..14924)
                     /locus_tag="MB022_001375"
     CDS             complement(14613..14924)
                     /locus_tag="MB022_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466381.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(14977..15372)
                     /locus_tag="MB022_001376"
     CDS             complement(14977..15372)
                     /locus_tag="MB022_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBT4466382.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(15408..15752)
                     /locus_tag="MB022_001377"
     CDS             complement(15408..15752)
                     /locus_tag="MB022_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466383.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            16053..16274
                     /locus_tag="MB022_001378"
     CDS             16053..16274
                     /locus_tag="MB022_001378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466384.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            16401..17177
                     /locus_tag="MB022_001379"
     CDS             16401..17177
                     /locus_tag="MB022_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBT4466385.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            17180..17368
                     /locus_tag="MB022_001380"
     CDS             17180..17368
                     /locus_tag="MB022_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466386.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAAPAELEKEK"
     gene            17370..17654
                     /locus_tag="MB022_001381"
     CDS             17370..17654
                     /locus_tag="MB022_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466387.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            17675..17863
                     /locus_tag="MB022_001382"
     CDS             17675..17863
                     /locus_tag="MB022_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466388.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            18188..18529
                     /locus_tag="MB022_001383"
     CDS             18188..18529
                     /locus_tag="MB022_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466389.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLWKQPRKPEYIYVIEDGLIDALEEYDRDNLIDISYYGQGKK
                     YIAEMEAEFDE"
     gene            18522..19268
                     /locus_tag="MB022_001384"
     CDS             18522..19268
                     /locus_tag="MB022_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBT4466390.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPQDVSMLEPASQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            19274..19960
                     /locus_tag="MB022_001385"
     CDS             19274..19960
                     /locus_tag="MB022_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466391.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            19963..20793
                     /locus_tag="MB022_001386"
     CDS             19963..20793
                     /locus_tag="MB022_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBT4466392.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            20810..21661
                     /locus_tag="MB022_001387"
     CDS             20810..21661
                     /locus_tag="MB022_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4466393.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            21658..>22009
                     /locus_tag="MB022_001388"
     CDS             21658..>22009
                     /locus_tag="MB022_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBT4466394.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEE"
BASE COUNT         6946 a         4035 c         4895 g         6133 t
ORIGIN      
        1 aaaactacag actggctaca gggaaagtac agatgcgctt gccataataa caatcgtacc
       61 agccacaaat cgtagtttta ttgcaaagga ggcattcgat caaatggaaa aaagcaacta
      121 tcattccaat gcggatcatc acaaacggca tatgaaacaa tccgtggaaa agcgggcttt
      181 tctatgtgcg ttcattattt cgttcacagt ctgcacgctg tttttggggt ggagactggc
      241 ttccgtattg gaggcaacac agataccgcc catccctgca actcatacag gcagcagcac
      301 tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc gccaaagaac agggagatga
      361 acaggaatgg agcctgattt tagtgaacag gcagaacccc atccccgcac agtacgatgt
      421 ggagcttgag caactatcaa atggtgagcg gatagatatt cggatttctc cctatcttca
      481 agatttgttt gatgccgcaa gaactgatgg agtttacccg attgtcgcat ccggataccg
      541 aacaacagaa aaacagcaag aaattatgga tgaaaaaatt gccgaatata aggcgaaagg
      601 ctacacctct gcacaggcta aagcggaagc agaaacttgg gtggccgtgc cgggaacgag
      661 cgagcatcag cttggtcttg ctgtggatat caatgcggac ggaattcatt caacaggcaa
      721 cgaggtttat agatggctgg atgaaaacag ctatcgtttt ggttttattc gtcgctaccc
      781 accagacaag acagagataa ccggtgtgag caacgagccg tggcattacc gatatgttgg
      841 catcgaagct gccacagaga tgtacaacca aggggtttgc cttgaggaat atttaaaacc
      901 agaaaaatga gaaaaggatg ttatgttatg gacagaaaaa gattgacaca gcgtttcccg
      961 ttcctgcttc caatgagacg ggcgcagaga aaaatgtgct tttatgcggg aatgagattt
     1021 gacggctgtc gctatgcgca gacgataggg gaaaaatcgc tttcccattt gctctttgaa
     1081 acggattgcg cattatacaa ccacaatacc ggatttgaca tgatatacca ggaaaacaag
     1141 gtgttcaact taaagctggc agcaaagacc ttaaacggct tattgataag accgggggaa
     1201 accttttctt tctggtggct ggtacgccat gcggacaaag atacccccta taaagacggc
     1261 cttacggtga ccaatggtaa actcaccacc atgtcgggcg gcggtatgtg ccagatgagc
     1321 aatttactat tttggatgtt cctgcatacg ccattgacaa ttatccagcg cagaggtcac
     1381 gaagtaaagg agtttccaga gccaaacagt gacgagatta aaggggtgga tgcaaccatt
     1441 ccagagggct ggattgattt aaaagtgcga aacgataccg actgcaccta ccaaatatgg
     1501 gtgaccttag atgatgagaa aatcatcggt caggtgtccg ccgacaaaga gccccaagca
     1561 ttatacaaaa ttacaaatgg cagtatccag tatgtccgtg aaagtggcgg gatttatgaa
     1621 tatgcccagg ttaaacggat gcaagttgcc ttaggtaccg gggaaataat agattgcaag
     1681 ctgctttata caaacaaatg caaaatctgt tatcccctcc cggaaagtgt ggatattcag
     1741 gaggagaacc aatgagaaaa agtgtgggca ttactgtttt tggatgcgaa caggatgagg
     1801 caaatgcttt ccgcgcttta tcgccggatt ttcatattat ccctacgctg attagcgatg
     1861 cgatatcggc agacaacgca aaattggccg ctggcaatca atgcgttagc gtaggccata
     1921 agtccgaggt ttccgaggcg acaattcttg cgctgagaaa ggtcggggta aaatacattt
     1981 ctacccgcag catcggctgc gatcacattg atacgactgc cgccgagaga atgggaatct
     2041 cggttggcac ggttgcgtat tcgccggaca gcgttgcgga ttatgctttg atgctgatgc
     2101 tgatggccat acggggtgca aaacccacca tgcacgccgt ggcgcaacaa gatttcagat
     2161 tggatcgtat ccgggggaaa gaactggggg atatgactgt gggagttatt ggaaccggcc
     2221 atatcgggca agcggtcgtc aaaaggctgc ggggatttgg atgccatgtg ctggcctatg
     2281 ataacagccg aaaaatggat gcagattatg tccagcttga tgagcttcta aaaaacagcg
     2341 atattgttac gctccatgtg ccgctttgtg cggatacccg ccatctgatc ggtcagaagc
     2401 aaattggaga gatgaagcaa ggcgcatttt taatcaacac cgcgcgcgga gcgcttgtcg
     2461 ataccggggc gctggtggag gcgttggaaa gcggaaagct gggtggtgcg gcactggatg
     2521 tgttggaggg cgaggatcag tttgtttata ccgattgctc gcagaaagtg cttgaccatc
     2581 cctttttgtc gcagctttta aggatgccga atgtgatcat cacaccccat acggcgtact
     2641 acactgagcg tgtgctgcag gatactacag aaaaaacaat caggaattgt ctcaattttg
     2701 aaaggagttt acagcatgaa tagaataaaa gtcgcaatca tcttcggcgg ttgctcggag
     2761 gaacatgatg tgtcggtaaa atccgcaata gaaattgctg cgaacattga tacggaaaaa
     2821 ttcgatccgc actacatcgg aattacaaaa aacggtgtat ggaagctatg caagaagcca
     2881 tgtacggaat gggaagccga cagtctcccc gccatactct ccccggatag gaaaacgcat
     2941 gggctgcttg tcatgaaaga aagcgaatac gaaacacggc gtattgatgt ggctttcccg
     3001 gttttgcatg gcaaattcgg ggaggatggt gcgatacagg ggctgtttgt attgtctggt
     3061 atcccctatg tgggctgtga tattcaaagc tccgcagctt gcatggacaa atcactggcc
     3121 tacattctta caaaaaatgc gggcatcgcc gttcccgaat ttcaaattat tgataaaggt
     3181 gacaagccgg aggcgggtgc gcttacctac cctgtctttg tgaagccggc acggtcaggt
     3241 tcgtcctttg gcgtaaccaa agtaaacggt acggaagaac ttaacgctgc gatagaagcg
     3301 gcaggacaat atgatggaaa aatcttaatt gagcaagcga tttcgggctg tgaggtcggg
     3361 tgtgcggtca tggggaacga ggatgatttg attgtcggcg aagtggatca aatccggctg
     3421 agccacggta tcttccgcat ccatcaggaa aacgagccgg aaaaaggctc agaaaatgcg
     3481 atgattacag ttcccgcaga cattccggtc gaggaacgaa atcgggtgca ggaaacggca
     3541 aagaaagtat atcgggtgct tggatgcaga gggcttgccc gtgttgatct ttttttgcag
     3601 gaggatggcg gcatcgttct aaatgaggtc aataccatgc ccggttttac atcgtacagc
     3661 cgctacccac gtatggtggc cgccgcagga atcacgcttc ctgcactgat tgacagcctg
     3721 attacattgg cgttaaagag gtgataagta tggaaaatgg ttttttgttt ttagatgaaa
     3781 tgttgcatgg cgtccgttgg gatgccaagt acgccacatg ggataacttc acaggaaaac
     3841 cggtggatgg gtatgaggtg aatcgcatca tcggcacaaa ggccgtggcg tttgctctgc
     3901 gcgaggcaca aatccatgcg gctgcgcttg gctatggctt gcttttatgg gatggatatc
     3961 ggccaagaac tgcggtggac tgcttcctgc gttgggcagc gcaaccggag gacaatctca
     4021 caaaagaaaa attttacccc aatatagagc gagccgagtt gattacaaag ggttatgtgg
     4081 cctcacaatc cagccatagc cgtggaagcg caattgatct tacgctctac cacctggata
     4141 caggggaact tgtttcaatg ggaagtaact tcgattttat ggacgaacgg tcgcatcata
     4201 cagcaaaagg gataggggat gcagaggcac aaaatcgaag atgcttgcgt aaaatcatgg
     4261 aaagcagcgg atttcagtct tatcgctttg aatggtggca ctataagttg attgatgagc
     4321 cataccccga tacctatttt aattttgccg tttcataatg aaagtattgg ttttgtatca
     4381 gattttggat ttgataaaat ctatgctggt catggacaat cttttactgg aaagtggtta
     4441 aagcatcttt tgtaatacat tgcaaattat agtattcaat ctgcccagcg gcagaaaaga
     4501 aaaaaaccgc tgttgggcag acctttccca cgcccatttt gtattttgct ggcggcggtt
     4561 ttgagaaatc aaggccgccg ttcttttctg gcagcaaaag gtgtggcggc ccataggagg
     4621 atgccgctat gctgttttgc tattgccatg ctctaatcca cttccaccac tcaaaaaagc
     4681 cttgcccctc cacagggcta ttccggttat gagtatgtac tataccatgt aaatcagcag
     4741 catagcaaag cattttctcg cccggtaggt ttgcgacctg ccgggcgatt tttgtcgtct
     4801 tttgcggccc ggctgtcccc aaaaaaattt tttgagaaat tttcaaaaag gaggtattta
     4861 gcgggtagct ggcggccttg tcaaagtctt gttagcggaa agggagagaa ccacctaagt
     4921 cccccacaga aagggggtga gaagatggat gctatccccc gcgactatga agcgcggtgc
     4981 atgttcgatg ctttttgcaa gacggtactg cgtaacgagg ccaaaagcta cttggctgaa
     5041 atgaagcgcc gccgtgaccg tgaagtgtca ctaagctctt tgtcacaggc agatttggac
     5101 aagctctgta cagtagatca ctaccccagc gacagcttta cgttttcgtc gcatggctat
     5161 gacctacata tcaacaatga gcttgttgcc gaagcctttg ccgcgctgcc cagcatggag
     5221 cagagtattt taatcctgca ctgtgttctg gatatggcag atggtgagat cggcggcctt
     5281 gtgggaatgt cccggagtgc cgttcagcgg cacaggacaa ataccttatc ggagctgcgg
     5341 aaacagctaa aggcgttgat gccgaaggga ggttagacgc ggatgaagaa cccgaactac
     5401 aagggcattg agcttttgcc cctttcggtg atcgaggccg cccgcgctgg ggatgccgga
     5461 gccgtagagc gcgttttgca gtattacagc ggttacatga acaagctgtg tacccggacg
     5521 ctctatgacg aaaacggcta tccgcatgtg tgtctggacg aggccatgaa gcgccgtctg
     5581 gaaatcaagc tcatccacgc tatcgtcacg aactgacgaa ccggccccgg cagcatgaaa
     5641 cggcttaccc tttccccgtt tcggtgtttt cccggtggcc tgatcgcacc ttgataaata
     5701 tgcccgcaag ataacggcgg cgcatcatag ggcatacgga cacattctgt ctgtgccgag
     5761 ccggacggct ggtgcgccat gatacttttc cctacgggaa aagcggagcg aggaacacca
     5821 cgccgcaatg tgagcgtggc ggcttgcggg cgacgacgta caggcaggac tatgatactc
     5881 ccttatagcc acagtccgag cgttaaaagc gtcgcaggca atgggtaggg ccgcgtgaga
     5941 accacgcggg ggtgaaagtc ccgtggagct gtgccagcag ccgtccgtct atgcccctaa
     6001 atcacacaaa cctttgaatt tgtgaaaggg ggctgcatta tgaaccatga tgtgcctatt
     6061 tgggaaaaat acacgctgac gattgaggaa gcgtctaaat atttccgtat cggagaaaac
     6121 aaattgcgta agctggccga ggaaaatccg gcctctggct gggtcattat gaatggcaac
     6181 cgcatccaga tcaaacgcaa gcagtttgag aaaataattg acactttgga cacaatctaa
     6241 tagtgaaaaa gggccgcaga tatgttataa taatttataa gcatatatgc ggctctttcc
     6301 gtcatggaaa ggagcatgac gagatgtcag aaaaaagacg agacagcaaa aatcgcgttt
     6361 tgcggtctgg agagagccag agaaaagacg gacgatatgc ttacaaatat gttgatacct
     6421 ttggaaaacc gcagtttgtt tactcatgga agttagtgcc tacggataag accccggcag
     6481 gtaaacggga ggatatttca ctccgggaaa aggagaaaga aatccagaaa gaccttgacg
     6541 acgggataga caccatcggc aagaaaatga ccgtctgcca gctctacgcc aagcagatca
     6601 ggcaccgggg caatgtgaag cacaacacca aactgggccg ggaacggctg atgcgtattc
     6661 tggaacaaga tcggctgggg agctgcccga ttgacagtgt gaagctgtcc gacgcaaagg
     6721 aatgggcgct tcggatgaaa gaaaagggcc tttcttataa gaccatcaat aacgacaagc
     6781 gttccctgaa agcggctttt tacaccgcca tacaggacga ttgcatccga aaaaacccgt
     6841 ttgacttcca gcttagtgac gttctggacg acgatacgga accaaaggtg cctcttacac
     6901 cagcacagga agaaagtttt ctgtccttta ttcaaggtga taaggtttac caaaagcatt
     6961 atgacgcaat cgttattctg ctggggacag ggcttcgcat ttcggaactg tgcggactga
     7021 cggacaaaga tttggatttt gaaaaccggg taatcatcgt gagccaccag cttttgagaa
     7081 ataccggcgt cggttactat attgacgaac ccaagaccca aagcggcgtc agaaaaatcc
     7141 ccatgaatga ggaagtgtat caggcgttcc agcgagtgat aaaaaaccgc aaaggtgcaa
     7201 agccgttcat tattgacggg tatgccaatt tcctgtttct caaacagaac ggctatccca
     7261 tgacggcggt agattatggc gggatgtttg gcaggcttgt gaagaagtac aacaagagcc
     7321 atgaagaagc gttgccgaaa acgacgacgc cccatgctat gcgtcataca ttctgcaccc
     7381 ggttagccaa tgcgggaatg aaccccaaag ccttgcagta cattatggga cattccaata
     7441 tcaccatgac gctgaactat tatgcacacg ccacattcga ttccgcaagg gcagaaatgg
     7501 aacggctggc agcctgacac aaggccggtt ttactactcc atttactact tttgaggggg
     7561 aaatggtgag aggtaataag aacatttgta agtatctaac aatatcaaaa atgccggaaa
     7621 agcccggaaa cacgggctat accgacattc aagaacttct aaaaagataa tctaaaatcc
     7681 catataattt ttcctcaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
     7741 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
     7801 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
     7861 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
     7921 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
     7981 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
     8041 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
     8101 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
     8161 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
     8221 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
     8281 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
     8341 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
     8401 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
     8461 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
     8521 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
     8581 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
     8641 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
     8701 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
     8761 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
     8821 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
     8881 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
     8941 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
     9001 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
     9061 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
     9121 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
     9181 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
     9241 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
     9301 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
     9361 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
     9421 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
     9481 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
     9541 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
     9601 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
     9661 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
     9721 ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct
     9781 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
     9841 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
     9901 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
     9961 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
    10021 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
    10081 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
    10141 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
    10201 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
    10261 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
    10321 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
    10381 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
    10441 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
    10501 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
    10561 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
    10621 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
    10681 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
    10741 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
    10801 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
    10861 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
    10921 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
    10981 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
    11041 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
    11101 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
    11161 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
    11221 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
    11281 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
    11341 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
    11401 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
    11461 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
    11521 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
    11581 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
    11641 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
    11701 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
    11761 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
    11821 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
    11881 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
    11941 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
    12001 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
    12061 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
    12121 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
    12181 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
    12241 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
    12301 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
    12361 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
    12421 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
    12481 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
    12541 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
    12601 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
    12661 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
    12721 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
    12781 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
    12841 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
    12901 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
    12961 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
    13021 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
    13081 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
    13141 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
    13201 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
    13261 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
    13321 aaaacaagtg tctgtttatc cagtagctca agaagcttaa taaataaact gctctatctc
    13381 ttgtgtagca agggatggag cttttttgta aattatggga ttttttttga ccatactttt
    13441 gaccatactt tcttagaagt taatataatt tgttagtttt tgtgttgttt cttctttttt
    13501 gctgttagta atgtgtgtat atgtgtccat agtagttttt atatttgcgt ggcccattct
    13561 ttcttggact tctttgtgat ctgcgcctgc ctcatatagc attgatgcat gtgtatgtct
    13621 aaaaccatgc agaccgatat aaggtaagcc ggcacgctca cgatatatat tgtatctttc
    13681 tgccactgac tgattaattg tgaaattatt ttcgtcattg gtaaacaaat aattagcttt
    13741 agtaaatcct aattgaaata agtactttct ttgttcgagc ttccaaaatt ttagttgctt
    13801 tatagttttt tcatctaaat aaattattcg atttgatttt ttggttttag gagtttcaga
    13861 tatataatat ttatctgaac gagctacagt ttttttaatg gccatttcat ttgttttaaa
    13921 attaatattg tcccaagtga gtgctaatat ttcgccgatt ctacatccgc tgaatgctaa
    13981 tagtctaaag agcgtatagt ctctgttttt aaaatatgga ttctgttctt gagatacttc
    14041 atttagaaat aattccaact gttcttttgt atataatttt aatttctttt ctgttttaat
    14101 ttgtggcttt ggaataagta aatttaatgt tggattatca gatgttaaac caatatttat
    14161 agcatactta aaaactttat tcatatagtt tatgaaaaga gggtactgct ttgaagttcc
    14221 tttttcactc caatcattta ctaccttttg acaatatgcc gtattaattt tagaaagctt
    14281 aatatttcca aatttgggat atatatgttt attaaagatt attttagtcc ttgaatatga
    14341 actttctttt acagtttttt tgtagctttc tagccataat tctgctgctt ctttgaaagt
    14401 agtatttgta cttgtgggca ttcctgtttt ttgtaattct agttctaacc tagaaagtgc
    14461 tgtttttgct tctttttgtg ttctaaatcc tcgtttagta gtatattttt tctttccagt
    14521 tagcggatca ataccgagat aagttttaaa gtaccatgct ttttcgccat ttttcttttt
    14581 atattgtttt atcattgcca tcaatattac ccctattcaa attctaattc tgccatgcta
    14641 tcattgaaaa attcgatcag tccttgttcc tctataaatt caagccatat ttccttcccg
    14701 ttaattaatg gaactccttt ttctttacga attgtttgat acaagttagt cacgacttgt
    14761 ttccacattt ctaatacagc tttatctatt ttcttatcat cccagcgttt tttttggggt
    14821 ctatttttgt ttttttcaat ttgataatta gtagctttga tgataaatgt ttcgtatccg
    14881 gttgtgcttt tttctaacca ttctttgaat tctgtatata tcatttttgt tcacctttct
    14941 atttttgaac gtatgttctt ttttgcttaa aaaattttag agagctgcat aacaatagat
    15001 gcgagctttt aataattctt tcacaagtgg ttcgaagcga acgcttaaat tgttatcttc
    15061 tataaatttc atgtaattga tattgtgtgg ttctaattca gactttgcaa tatatctatc
    15121 taataatttt tcaatcatga agcgatcagc ctcatattcc atttttgaat ggagagagaa
    15181 tgctaagtta tataaaaaat aattatcatg atgtttagaa gcgtgtccta attcatgtaa
    15241 taaggttctt tgtcttctat acttagataa attagctttt attactatgg tattcataca
    15301 agcaacataa tggccatcgg catcaaggtt ttcacgctct tctaccttaa cgccgagttc
    15361 attaattatc atttcaattt gactgtccaa atgactcacc tacttattta gttatctttt
    15421 ttctctaaat atgcttcaat catagctgat agtatttccc tatcattatc ggtgagcggt
    15481 tttccgtcgc tactcataac agatgctaaa gcttcttcca cggttagttt tctttcctct
    15541 ggtgcaactc ccgcgttagg atcatctgtg cgtcctagca aataatctgt tgatacatgg
    15601 aagtagtcag caactttttg caaagtttct attttaggac tttgtgtctt ccatttataa
    15661 atagagtttt ttccaaatcc tattctattt tctaattcag aaatagaaat acctcttttt
    15721 ttacagagct ctttaaccct ttcgaaaagc atgtcaaatc aaccttccta gagcgtacga
    15781 taataattta tacaaaaaga tagaaaggta gttgacaaat ctatccaatt gtcttatatt
    15841 taactcgtaa gctaatttaa taagctaaca agcaacaaga aatacaccta ataaaataaa
    15901 cgaattcgag gtcggcaaac taagaatgtt aatttttagg gtttttctag gcttatttaa
    15961 ctgtatataa atataagaca gttggataga aatgtcaata aaaattagct aattttatta
    16021 gcttacatat ttaaagaaaa ggaggaaaca tcatgaaaca aaataaaaaa actccttccg
    16081 caaaaggagc tttaataact ttaaatttta aagaccaaaa gtttagtgct gttgttgatg
    16141 aaattcatcc acaagagcat caaaaaaatt cttttgcaac tgacgagaaa aagactcaga
    16201 aacgctctga gaaagttctt cgaataattc aatttctgtt ggaaaagatt cgtcattttg
    16261 gaagtctatt ttgaattctt tattaacagt cttgaatacg gtttgtttga ataaggcgtc
    16321 gacatccata ttaaattctt ccatactatt ttcacctcgc tttctattca aaattatatc
    16381 aaagaaagga atgagcaaaa atgaacacac cgcaaatttt taactttgaa caacatgaag
    16441 taagaacagt aacaatacat gatgaaccat tttttgttgg aaaagatgtg gctaaagttc
    16501 tagggtacca aaatggtagt agagatatta acagacacgt cgatgtagaa gatcggcaaa
    16561 actaccaaaa cggtactttt gaatcgccta gagggttaac tattattaac gaatcaggtt
    16621 tgtactcctt aattttagga agcaaacaac caaatgctaa gaaatttaaa cgttgggtga
    16681 ctagcgaagt gttgccagca attagaaaac atggaggtta tctaactcca gaaaaagtag
    16741 aagaagcttt gcttaatcca gatacaatca tccaattagc aactaagttg aaagaagaac
    16801 gtactggaag actaatcgca gaacaaaaaa ttgcagaata cgaacccaaa atttcgtatt
    16861 tggatagtat cttatcttct acagattcag taactattag tcaaatcgcg gctgattatg
    16921 ggatgtctcc acaacagatg aacaaactac ttcataagct aggtattcag aaaaaagtag
    16981 gcaaccaatg gttattatgc aaaaaacaca tgagacaggg ttacacaaaa tctcatacaa
    17041 ctgagattcc gaaatctgat ggcggaacaa aagttgtgat gaatacaaaa tggactcaaa
    17101 aagggcgtct gttcatttat gagtcgctga aaaaagaagg atatattcca gaaatcgatt
    17161 tattagagga ggaatgacga tgtcacaaga atttattttg aaagtcagaa ttcaattagc
    17221 aaaatacgga aagtctcaga actggttagc tgatactatc ggaatttcta gaccatatat
    17281 gtcggacatt atgaatgggc gtagaaaacc agataaacaa atcaagccaa ttgaggcagc
    17341 accagcagag ttagagaagg agaaataaca tgcaaataac aattccagat aatttagtag
    17401 tttccgaatt aactacacag attacgaatg ctgttcttaa ttcattggac gaacgattac
    17461 accttatgaa caaatcagta gagcttcctc catatccaaa caaatcagag gtaaaaaaag
    17521 ttttaggcat tggtgatgac aaattaacac attggataag cctaggctta aaaacacagc
    17581 agtggagcaa gttagacatc agaattgaac gatcggaact ccaaagattt ttgaaagaaa
    17641 actttgagtt ctaaaggcaa aggagaatga ttttatgtcc tacacattgc aacaagaaca
    17701 tcaaattcta ggtctgatta aacagcgtag aaaacaatta caagatgatc gtgcagcgct
    17761 tagaaaatcc gatgagctat cagatagaca agctgaacta attgcttctg aacttgagga
    17821 tttgagaatg ctagaaataa aaaataggga gattagatta tgaagaagac agacacactt
    17881 tttataggat tcattttggg gttattagtg attgtagcgc accaaagtat tatcggagga
    17941 agcttgttcg cagcattgat ggttttaatc aatctgcttg actcaaaaga aaggagcaac
    18001 tatggcacga gaagaagcgc taaaaatcgg taaagtgatt gctgataatt ggtgggcaaa
    18061 tagccgttct attattttaa gcaagcaaca tatcgaaaag aaaaaagcat ggcaacaaat
    18121 aaaaaagtga ctccgccgac caaagcaatg agtcgcaaac aaaatacatc taaggagatg
    18181 ttaccacatg aaaaaagaac tttccactct agatcaatat ttgactgatc ctagttgggg
    18241 caaatcgaat atcaaggaaa caagcaatcg aaaaatcaga cgtaatcttt tgacggatga
    18301 agaactagca tgtgatcaag acgatttggg aaattttgtg agtatttggg gacatgtcta
    18361 ccttatccat ctatggaagc agccaagaaa acctgaatac atctatgtca tcgaagatgg
    18421 cttgattgat gcactagaag agtacgacag agataacttg attgatatct cttattacgg
    18481 acaaggcaaa aaatacatcg ctgaaatgga ggcagaattt gatgagtgaa aaggaacaac
    18541 ctttgaaaaa tagaagtgat aatacgcttt ttaatacctt atacaaaata aatgtgaaag
    18601 atgttactga aaaacgaaat aacctcactt atctttcgtg ggcatgggct tgggcagaag
    18661 ttagcaaagt gtgtgaggca gtagattatg aaatctatca tgatccagaa acatatctgc
    18721 cgtatgtctt tgataagaaa acagggtaca tggtttttac cagtatcaca gtcaacggag
    18781 taaagcgtga catgtggtta ccagtcatgg atggtgcaaa caaggcaatg aaagatgagc
    18841 catataccta cgaagtcaat gattatcagt ggaataacga aacgaagaaa aaagagattg
    18901 ttggaaaaat cgaaaagcga gttgaagcag caactatgtt tgatatcaac aaaacgatca
    18961 tgcgctgtct tgtaaaaaat ctagcaatgt ttgggctagg gctatatata tttgctggcg
    19021 aagatatgcc acaagatgtc tcgatgcttg aaccagctag ccaaagaagc aaaaagctat
    19081 tcttagatgc tttacaactg gttgctaaca agtacgaaaa atcgattgat gaagcaattg
    19141 ttgcattgac tgatgcagct tccataaccg ccgatgacag taaatggacc aagagagact
    19201 tgggcattct aaaacgaggc gttaactgga ttgaagatca gtacagagaa gaaacaaaag
    19261 agaagtgata tgagtgttta aaccattaat cgattcatat tcagcggttc tgaaaaagtt
    19321 caaaggaaaa gacataggtg caacgatcaa tgaagaagtg aacattgatc gactaaagac
    19381 gatgtatgac ggctacgatg gcgatcgagt cattgaaatt cgttttattg atcctagacg
    19441 tttcaccgta cagcaacgaa acttcatcta tgcgctgata ggcgatattt ttatcgatac
    19501 aggcatgcca acggacttct ggaaggaatt cttctacttc cgttttgaag gcgtcacagg
    19561 gcgcaaaata agcctcaaag acgaatcgaa tacgactgtg agtgatgcta atgtcttagc
    19621 aaatatcatt ttagatttca tctttgaaca ccatattcct ttcaaagaag gctatgagat
    19681 tttaccagcg aatcaagagt attacttcta caaatgcatc acaaaaagag tctgctgcat
    19741 ctgtggcaaa acaggagctg atatcgatca ctttgacaaa gcgctgggaa gacgaaagcg
    19801 caaagaagtt gatcattcag agtacacatt tgcagcactc tgcagaatcc atcacacaga
    19861 gaaacacaaa ataggtgtga tcaatttcaa aaataaatat caaatcaaag ggatcaagtt
    19921 aaaccaggaa acaattaaaa agttaaggat aggaggataa atttggctga gataagttgg
    19981 atcaaactta aaactactat gtttgacgat gaaaaaatac gattaatcca agctgttcct
    20041 gagtcggatg ccatcatcgt tatatggatt cgattactag ttttagcagg aaagactaac
    20101 gacgatggtc tgatatatat ccagaggaac atgccttata ccgaagaaat gcttgctaca
    20161 ttgtttggca aaaacgtaaa tacggttcgc ttagcgctaa ctacattggc aaatttcaac
    20221 atgattgatc taagcagtga tggactaatt gccatcagta attgggaaaa acatcaaaat
    20281 atcgagggta tggataaagt aaggctaaaa aatgctgaaa gaaaccgtaa atacagggaa
    20341 agaaagagac aggaacgtct caaattggaa aatgacgtta gcgtgacgtc acgtgacggt
    20401 acagataaag atatagaaga agataaagat atagataaag aagaaaagaa aggtaagtat
    20461 tctgacgaac acttacgcct tgctaaaaag ttgcaaagta atttaactga agattttcca
    20521 aaagaaatga acaaagtaga tatcgaaaaa tgggcagaca caatcaggtt gatggaagaa
    20581 agagataaag catctataga agcgattgag tatgtgatca attggctacc tacaaatgaa
    20641 ttttggtttg gaaatattag aagtgctaag aaattgagag aaaaatttga gaagctcaaa
    20701 ttcgaaatca aagcagacaa gaagaatcat aaaaagcaaa gtcaaaaact acagtacagc
    20761 aatcctagtg aatatgacga cttgccaatt taaaaaggag atgcatcaca tggaaagcct
    20821 agcaaatgct atggagaaac taataagaag agtattagtg caaagcggaa aatgtccaga
    20881 atgtagcgaa cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa
    20941 accaacatgc atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga
    21001 acggatatat aacgatagct taaaagcacg agcgttgagt ttttttcaaa atggttcggt
    21061 attaacagat aaaactttgt ttaaatgcaa aatggaaaat tatcacgtag tggatcaaga
    21121 aacaaaaatc gctttagaaa aagcaaaaag ctatacgaat gaggttctgc tgaaccatcc
    21181 tgcacatttc attctatcag ggaaatcagg aagcggaaaa agccacttat caatggccac
    21241 agcttgggaa atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca
    21301 agaattatta gagcaaataa agttttctta taacaatact gaactgagaa aagaaattga
    21361 aggatcgctt atagccgata ttaaaacaac tgatttggtg gtttttgacg atattggagc
    21421 tgaattaggt agtggggtat caaatagtag gcagtttaca aacaacacgt taaacacgct
    21481 cttagaagcc agacagaaca aggcaacgat catcacaaca aacttatctg gtcctgaact
    21541 aagagaagcc tacggtgaaa gaattgtttc taggatattt aagaattcag aaggttatgc
    21601 gctgaaattc caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg
    21661 aataaatacc gtaataaaaa aactgttcat cgaggtatca agtttgattc tatcgcagaa
    21721 gcagagtatt acgatctagc cttgtggcaa gctgaagcaa atggctggaa agtaaaactt
    21781 caggaaagat ttgagctgat gccgaaattt gaactagacg gaaagaagta tcgcaagatc
    21841 gagtatattc ccgacttcac attttataaa aacggcaaac ttgtcaaagt cgtagatgtc
    21901 aaaggaatgc agacaaaaga ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa
    21961 gcgccgttga tattagctaa aaaatatcgg aatacgttca aggaagagc
//
LOCUS       DAGCXG010000035        21187 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGCXG010000035 DAGCXG010000000
VERSION     DAGCXG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.036"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            21..938
                     /locus_tag="MB022_001389"
     CDS             21..938
                     /locus_tag="MB022_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4466395.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            941..3118
                     /locus_tag="MB022_001390"
     CDS             941..3118
                     /locus_tag="MB022_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4466396.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3115..4116
                     /locus_tag="MB022_001391"
     CDS             3115..4116
                     /locus_tag="MB022_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4466397.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4113..5045
                     /locus_tag="MB022_001392"
     CDS             4113..5045
                     /locus_tag="MB022_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4466398.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5290..5406
                     /locus_tag="MB022_001393"
     CDS             5290..5406
                     /locus_tag="MB022_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBT4466399.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5422..6237
                     /locus_tag="MB022_001394"
     CDS             5422..6237
                     /locus_tag="MB022_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4466400.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKKTTSCIYTPL"
     gene            6302..7342
                     /locus_tag="MB022_001395"
     CDS             6302..7342
                     /locus_tag="MB022_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBT4466401.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            7461..7628
                     /locus_tag="MB022_001396"
     CDS             7461..7628
                     /locus_tag="MB022_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBT4466402.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7688..8041)
                     /locus_tag="MB022_001397"
     CDS             complement(7688..8041)
                     /locus_tag="MB022_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466403.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            8546..8968
                     /locus_tag="MB022_001398"
     CDS             8546..8968
                     /locus_tag="MB022_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4466404.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            8965..9195
                     /locus_tag="MB022_001399"
     CDS             8965..9195
                     /locus_tag="MB022_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466405.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9656..9859
                     /locus_tag="MB022_001400"
     CDS             9656..9859
                     /locus_tag="MB022_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBT4466406.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9941..11158
                     /locus_tag="MB022_001401"
     CDS             9941..11158
                     /locus_tag="MB022_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4466407.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11390..11716
                     /locus_tag="MB022_001402"
     CDS             11390..11716
                     /locus_tag="MB022_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4466408.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11784..12497
                     /locus_tag="MB022_001403"
     CDS             11784..12497
                     /locus_tag="MB022_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBT4466409.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12807..13658
                     /locus_tag="MB022_001404"
     CDS             12807..13658
                     /locus_tag="MB022_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4466410.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13779..15644
                     /locus_tag="MB022_001405"
     CDS             13779..15644
                     /locus_tag="MB022_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4466411.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15692..17101
                     /locus_tag="MB022_001406"
     CDS             15692..17101
                     /locus_tag="MB022_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBT4466412.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            17209..17910
                     /locus_tag="MB022_001407"
     CDS             17209..17910
                     /locus_tag="MB022_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4466413.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            18069..18548
                     /locus_tag="MB022_001408"
                     /pseudo
     CDS             18069..18548
                     /locus_tag="MB022_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            19396..19971
                     /locus_tag="MB022_001409"
     CDS             19396..19971
                     /locus_tag="MB022_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBT4466414.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19971..20411
                     /locus_tag="MB022_001410"
     CDS             19971..20411
                     /locus_tag="MB022_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4466415.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            20427..21128
                     /locus_tag="MB022_001411"
     CDS             20427..21128
                     /locus_tag="MB022_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4466416.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         7080 a         3599 c         4489 g         6019 t
ORIGIN      
        1 gtggcagatg gaaagaaacc ttgccagaga tttcccatga aatcaatatc gtcactctga
       61 cttctgatga gaaaaacaaa ggcttacttg acccttatgt gattatgaaa aatcccaaag
      121 attctgaatc actggctatt gatattctga cattccttac ggggatttcc tctcgtgatg
      181 gggaacgctt cccaatcctt agaaaagcca ttcgtgcagt aaccaatagt gaagtacgag
      241 ggttgatgaa agtgattgag gaattacggg ttgagaatac gccactaagt accagtatag
      301 ccgaccatat cgaaagtttt acagactatg actttgcaca tttattattc agtaatggtt
      361 atgtggagca gtctatcagc ttagaaaaac aactgaacat tatacaggtt gcggacttgg
      421 tacttcccga caaggaaact tcctttgagg aatataccac tatggagctt ttatccgttg
      481 ctatgctgat tgtcattagt acctttgctt tagactttat ccatacagac cgaagcattt
      541 tcaagattgt agatttagac gaagcatgga gctttttaca ggtagcacaa ggaaaaacac
      601 tatctatgaa gctggttcgg gctggtcgtg ctatgaacgc tggggtatat ttcgtgaccc
      661 aaaatacaga cgacctctta gatgaaaaac tgaaaaataa cctcggctta aaatttgcat
      721 ttcgttccac tgaccttaac gagattaaaa agaccttagc cttttttggt gtagacccag
      781 aggacgaaaa caatcagaag cgattgcgtg atttggaaaa cgggcaatgc cttatcagtg
      841 atttatatgg tcgtgtcggt gtgatacagt tccaccctgt atttgaagaa ctgctccatg
      901 cctttgatac cagaccacct gtgcgaaaag aggtgtaaat gtgaaaccat caatagtaaa
      961 cagaataaaa tcaaactgga cgctgaaacg tctaggtaaa gtggcaatga cagtggcttt
     1021 cacacttgtg attgccattt ttcttttagc catgctggga acggtggttc aagctgcggg
     1081 cttggtagat gatacggtca atgtggcaaa tgaatacagc cgatacccac ttgaaaacta
     1141 tcaactggat ttttatgtgg ataatagctg gggctggctt ccgtggaact ggtcggacgg
     1201 gattggaaaa caggtcatgt atggactata tgccattacc aattttattt ggacaatcag
     1261 tttgtatgtt tccaatgcga caggttactt agtacaggaa gcctattcct tagacttcat
     1321 ttccgctaca gcagattcca ttggtaagaa tatgcagacc ttagctggtg tgagtgcaaa
     1381 cggattttca acagagggtt tctatgttgg attcctctta ctcttgattt tggttcttgg
     1441 ggtttatgtt gcctatacgg gactgataaa gagagaaacc acaaaggcaa ttcatgccat
     1501 tatgaatttt gtgctggtgt ttatcctatc ggcttccttt attgcctacg ctcccgacta
     1561 cattaaaaaa atcaatgact tttcatcaga catcagtaat gccagtttat cacttggcac
     1621 gaagattgtc atgccccatt ccgatagtca aggcaaggac agcgtggact taatcagaga
     1681 tagcctgttt tccatacagg ttcagcaacc gtggctactg cttcaataca acagttcaga
     1741 cattgaaagt atcggtattg accgtgtgga aagcctgctc tccaccagcc cagattccaa
     1801 caatggcgaa gacagagaaa aaattgttgc ggaagaaatt gaagacagaa gcaataccaa
     1861 tctaaccatt acaaagacca ttaaccgttt aggtacagtc ttcttcctat ttgtcttcaa
     1921 tattgggatt tccatatttg tattcctatt aacaggaatc atgattttct cgcaggtact
     1981 ttttatcatc tatgctatgt ttctgcctgt gagctttatt ttaagcatga ttccatcatt
     2041 tgatggtatg tcaaaacgag ccataacaaa gctctttaat accattttga cacgagctgg
     2101 aatcacattg attattacga cagcatttag tatttcaacc atgctctata ccttatcggc
     2161 tggttatccg ttctttttga ttgcttttct acagattgtg acctttgcag gaatctactt
     2221 caagctgggc gatttaatga gtatgttttc tctacagagt aacgattctc aaagtgtggg
     2281 aagtcgtgtg atgagaaaac ctcgtatgct tatgcacgct cacatgcacc gtctacagcg
     2341 gaaacttgga cgttccatga ctactctagg ggctgggtct gccattgtta caggtaaaaa
     2401 aggacagtcg ggttcgggga gttctgcaag gacacaagca gatcactccc gaccagacgg
     2461 aaaggaaaaa tcaacacttg gaaaacgtat cggtcaaacc atcggtacag tagctgatac
     2521 caaagacaga atggtagaca ctgctagtgg tttgaaagaa caggttaaag atttgccgac
     2581 caatgcaaga tatgcagtat atcaaggaaa atccaaagta aaagagaatg tccgtgattt
     2641 aaccagtagt atttctcaaa ccaaagcgga cagagccagt ggacgcaagg aacagcagga
     2701 acaaaggcga aaaaccattg cgaagcgtcg ctctgaaatg gaacaggtca aacagaaaaa
     2761 acagcctgct tcttctgttc atgaaagacc gactacaaga caagaacaat atcatgatga
     2821 acagacctca aaacagtcta atattcagac ttcatataag gaatctcaac aagccaaaca
     2881 agagcgtcca gcagttaagt ccgatttttc aagtccaaaa gtggaacgcc aaggcaatac
     2941 cgttcaagaa aaaaccgttc aaaagccagc aacttcaacc actacagcag atagaacttc
     3001 acaacgtcca atcacaaaag aacgtccgtc tactgttcaa agagtaccac tacaaaatac
     3061 aagaagtaga ccaccaatca aaaccgccac cattaagaaa gtcggtaaga aaccatgaag
     3121 ttgaaaactt tagtgattgg tggttctgga ttattcttga tggtcttctc actgcttctg
     3181 tttgttgcca ttttattttc agatgaacag gacagcggaa tttccaatat tcattatgga
     3241 ggtgtgaatg tttccgcaga agtgctggct cataagccta tggtagaaaa atatgccaaa
     3301 gaatatggcg ttgaagaata tgtcaacata cttcttgcga ttatacaggt ggaatcgggc
     3361 ggtactgcgg aagatgttat gcagtcctcg gaatccctcg gtcttccacc taattcattg
     3421 agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgaattatt agccagtagc
     3481 gaaaggctca gtgtagattt agaatcggtt atccagtcct acaattatgg tggtggtttc
     3541 ttagggtatg tggctaatcg tggaaataaa tatacctttg aactggctca aagtttctca
     3601 aaagagtatt caggtggcga aaaagtgtct taccccaatc ccatagccat acctatcaat
     3661 gggggctggc gatacaacta tggcaatatg ttttatgtgc aactggtaac gcagtatctt
     3721 gtcacaacag agtttgatga tgatacggta caagccatca tggacgaagc actgaaatat
     3781 gagggctggc gatacgttta cggtggagct tccccgacta cttcttttga ttgtagcgga
     3841 ctgacacaat ggacgtatgg aaaagctgga attaacttac cacgaaccgc acaacagcaa
     3901 tatgatgtga cccagcatat cccactatcg gaagcacaag ctggcgattt ggttttcttt
     3961 cattctacct ataacgctgg ctcttatatt actcatgttg ggatatacct tggcaataac
     4021 cgtatgtttc atgcaggcga cccaatcggt tatgccgact taacaagccc ctactggcaa
     4081 cagcatttag tgggagcagg acgaatcaaa caatgagaaa ggaagattta atgatgaaat
     4141 ttagaaaaaa tcagaataaa gaaaaacaga taccaaagga aaagaaacct cgtgtctata
     4201 aggtcaatcc tcataaaaag gttgtgattg ccttgtgggt acttttaggg cttagtttca
     4261 gctttgcgat attcaagcac tttacagcta tagatactca tactattcac gaaacaacta
     4321 tcatagaaaa ggaatacgtt gatactcatc atgtagaaaa ttttgtagag aactttgcga
     4381 aagtctacta ttcatgggag caatccgata agtccattga taatcgaatg gaaagtctaa
     4441 aaggctatct gacagatgaa cttcaagctc tcaatgttga tacagtacgc aaagatattc
     4501 ctgtatcgtc ttctgtaaga ggatttcaga tatggacggt agagccaact ggcgacaatg
     4561 agtttaatgt aacctacagt gtagaccagc tcattacaga gggagaaaat acaaagaccg
     4621 tccactctgc ttatatagtg agtgtctatg tagatggttc tggaaatatg gtactggtta
     4681 agaatccgac cattaccaac atacctaaga aatcaagtta taaaccaaaa gccattgaaa
     4741 gtgaggggac ggttgattcc attacaacca atgaaatcaa tgagttttta acgacgttct
     4801 tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat gacgggatat
     4861 taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt cacaatcgta
     4921 aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag accaaagcaa
     4981 cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg aagattatag
     5041 aataacaaat attggtacat tattacagct attttgtaat cacgtactct ctttgataaa
     5101 aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta tctatttaaa
     5161 aggagttaat aaatatgcgg caaggtatta ttaaataaac tgtcaatttg atagcgggaa
     5221 caaataattg gatgtccttt tttaggaggg cttagttttt tgtacccagt ttaagaatac
     5281 ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat gcctatggtt
     5341 atgcataaaa atcccagtga taagagtatt tatcactggg atttttatgc ccttttgggc
     5401 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
     5461 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
     5521 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
     5581 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
     5641 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
     5701 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
     5761 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
     5821 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
     5881 atcaaacaga aggtagaact gtatcctaat gtgtgtgtga cgaactttac cgaatctgaa
     5941 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
     6001 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
     6061 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
     6121 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
     6181 ttcaaaattg aatatacaaa aaaaaagaca acgtcttgca tatatacgcc tttatagtgg
     6241 agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga
     6301 aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat
     6361 tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt
     6421 gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag
     6481 taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc
     6541 gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa
     6601 agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact
     6661 aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca
     6721 catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc
     6781 gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt
     6841 tcaaaatgca gttatggaag ggatacgcta tggttgtgaa caaggattgt atggttggaa
     6901 tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg ttagtacccc
     6961 agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa aagctggaac
     7021 agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat atctttcacg
     7081 agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat tgaaaaataa
     7141 tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc gtagtgattt
     7201 aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt accatgttac
     7261 taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata aagtacgata
     7321 tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg tttcttgtta
     7381 aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag gagatagtta
     7441 ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa
     7501 gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga caagaaaatt
     7561 taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca aagacgcaga
     7621 gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta tgtatggatt
     7681 cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa gattccatta
     7741 tggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat cgtcgggtgc
     7801 tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga agtaacgata
     7861 caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata ttagttaagt
     7921 accgtgggtc aatttcaatc aatgctccca cttgttcacg agttaaacct cgtttcaatc
     7981 gagcttcttt aatggctaaa ccaaaggctc taaaatcata tttatcttct tttttacgca
     8041 tagtagacca cctctataca ttttattgtt cctactgaat taaaaacagg tatagaaaaa
     8101 cgtgttatat ggtttatagg tttatattta ataaaaagca ctactaaacg ccaataaaaa
     8161 aaaccgttat atggtagtgc tatttacgct gttaaaatat tgtatattac ttccaaatgg
     8221 cggtttgttg gaggtcaacg tcgccatgaa gtacatcata tacaataaat ttccttacat
     8281 tgggttcttg tcaaaaaaag tcgtctatct gcaatagata agtacgtcca ccaatgtggt
     8341 tttataaatc atatagatag aataacagaa gcatgtaaac agagaaataa atctgtttat
     8401 atgctttttt ggctattcag aactttttta caaagtttat ttatcagtaa tgcaacaaat
     8461 ccccctttca cattgggact aagagtgaaa ggagataaac gagcaaggct cacttccttt
     8521 cctagacaga aagggggtga gaaacatgaa accatcttct tttcagacca caatagaaaa
     8581 tcagtttgac tatatctgta aacgtgctat ggaagacgag cgaaagaatt atatgcttta
     8641 tctttcaagg attgcaaagc gtgaggtgtc cttttcggat gttggcgatt atcttgttag
     8701 ccagtttgcg acaacagata actattcaac tgactttcag atttttacac tcaatgggtt
     8761 atcagtaggc gttgaaaatg atttgttgag tgaagcatta cgtgagttgc cagacaagaa
     8821 acgtgaaatt ctactgctgt tttactttat ggacatgagc gattcagaaa ttgcagacct
     8881 gttgaaattg aaccgttcta ctgtctatcg gcatagaacc agtggactag ccttaattaa
     8941 aaagtttatg gaggaatttg aagaatgaaa acacaatatc ctatgattcc ctttcctctc
     9001 attgtaaagg caacagatgg cgataccgaa gcgattaacc agattctaca tcattacaga
     9061 gggtacataa cgaagcgttc cctacgactt atgaaagatg aatatggcaa tcaaagtatg
     9121 gtcgttgatg aagtcttacg tggaagaatg gaaaccagac tgattacaaa gattttgtca
     9181 tttgaaatta agtaatatcc tctctccttt cgtggaagcg tgctaaacca ttccacgctt
     9241 cccgaacagg gaggtttgtt attccaccaa agcatattga gctttcaatg tgttttgata
     9301 ggctaacgag ccattgttct ttgaaaactg aataaaagta atcgaatacg tttcgataag
     9361 aaaagagcca acggaactaa ccgccatgac ctatcttata aagatagcga gcgattcatg
     9421 ttagtgatcc gagaagcaat ctttagcagg attgcctgca acgacattct tatcgtgata
     9481 atgatactcc catacagtca atagtccgag cgtgataaaa ccgtcgcagg caatgagtat
     9541 ggctacatga gaaccatgca ggggtggaac tcccgtgagc tttgctaaag ctgttcgatt
     9601 gctggtaaaa caacttttat gaaatccaaa taagtgattt ggaaaggagg attttatgaa
     9661 gcagactgac attcctattt gggaacgtta taccctaacc attgaagaag cgtcaaaata
     9721 ttttcgtatt ggcgaaaaca agctacgacg cttggcagag gaaaataaaa atgcaaattg
     9781 gctgattatg aatggcaatc gtattcagat taaacgaaaa caatttgaaa aaattataga
     9841 tacattggac gcaatctagc gtcgccaaag ggtcttgtat atgataaaat agtattaagt
     9901 cgtatcaagg ctctttccat aaaggaaagg agcaaatgcc atgtcagaaa aaagacgtga
     9961 caatagaggt cgaatcttaa agactggaga gagccaacga aaagacggaa gatacttata
    10021 caaatatata gattcatttg gagaaccgca atttgtttac tcgtggaaac ttgtggctac
    10081 agaccgagta ccagcaggaa agcgtgattg tatctcactt agagagaaaa tcgcagagtt
    10141 acagaaagac attcatgatg gtattgatgt tgtaggaaag aaaatgacac tctgccagct
    10201 ttacgcaaaa cagaacgctc aaagaccaaa ggttagaaaa aacactgaaa ctggacgcaa
    10261 atatcttatg gatattttga agaaagacaa gttaggtgta agaagcattg acagtattaa
    10321 gccatcagac gctaaagaat gggctattag aatgagtgaa aatggttatg cttatcaaac
    10381 catcaataac tacaaacgtt ctttaaaggc ttcattctat attgctatac aagatgattg
    10441 tgttcggaag aatccatttg actttcaact gaaagcagtt cttgatgatg atactgtccc
    10501 taagaccgta ctaacagaag aacaggaaga aaaactgtta gcctttgcaa aagctgataa
    10561 aacctacagc aaaaattatg atgaaattct gatactctta aaaacaggtc ttcgtatttc
    10621 agagtttggt ggtttgacac ttccagattt agattttgag aatcgtcttg tcaatataga
    10681 ccatcagcta ttgagagata ctgaaattgg gtactacatt gaaacaccaa agaccaaaag
    10741 tggcgaacgt caagttccta tggttgaaga agcctatcaa gcatttaagc gagtgttagc
    10801 gaatcgaaag aatgataagc gtgttgagat tgatggatat agtgatttcc tctttcttaa
    10861 tagaaagaac tatccaaaag tggcaagtga ttacaacggc atgatgaaag gtcttgttaa
    10921 gaaatacaat aagtataacg aggataaatt gccacacatc actccacata gtttgcgaca
    10981 tacattctgt accaactatg caaatgcagg aatgaatcca aaggcattac agtacattat
    11041 gggacatgct aatatagcca tgacgctgaa ctattacgca catgcaacat tcgattctgc
    11101 aatggcagaa atgaaacgct tgaataaaga gaagcaacag gagcgtcttg ttgcttagta
    11161 gtacaaatga atttactact tatttaccac ttctgacagc taagacatga ggaaatatgc
    11221 aaagaaacgt gaagtatctt cctacagtaa aaatactcga aagcacatag aataaggctt
    11281 tacgagcatt taagaaaata taaaaagata attagaaatt tatactttgt tttatgtaaa
    11341 aaaatggtat gattaatcaa gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat
    11401 ggaaaaagtc aaccagttta gagatgaacg taattggcga caatttcata acgaaaaaga
    11461 tttggctatc tctatttctt tggaagcatc tgaattgtta gaactttttc aatggaaaac
    11521 gccggaagaa gtaaaagaaa cacaattaga acgaatcaaa gaagaactgg cagatgtatt
    11581 gatctattca tacatgatgg cagacaacct tcacctggat ctagatgaga tcattgaaga
    11641 aaaattgatc aaaaataatt tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa
    11701 atacacagag ttgtagaaat gtgtcattcg gctgttggaa agagcaaaag aaaaacagta
    11761 aaatgagtct ggagggagag aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca
    11821 atatcctctt gttcctcttg ttattagtag gactagcttt gattttcaat gaacagatca
    11881 aggattattt tgtcagagag acaggagata aatatgctat agcgaatgta acaaaagaag
    11941 atctgaaaaa gaataacgat aaagatgtca gttttgactt tgatgcggta gaaccaatga
    12001 cgacagaagg ggtcatgagg tcgcaaatga gaggaacaga tctaccagtg attgctagta
    12061 tcgcagtccc ttcagtttca atcaatttac cggttttcaa aggcttggat aatacgtcac
    12121 tactttacgg agcaggaaca ttatctccag atcaagaaat gggaaaagga aattatgcct
    12181 tagcaagcca tcgtgcaacg aaccctgaat tgctgtttac accgctagaa aatttagaga
    12241 tgggtgccaa gatctatttg acagatttag aaaatgtgta tacatacaaa acatttttta
    12301 aagaaaaagt tgctcctaca gatacccaat tactgaatga agtcgaggga aaagaaattg
    12361 tcacactaat tacttgtggc gacatggacg ccgtcacgcg actagttgta caaggagaac
    12421 tggaaagtgt tacatcgata aaagatgcga cagatgatat gagatctgct ttcaatctag
    12481 agacaaaaac attttaggat actgggacat aagtcgttac agattaaaaa cagccaaagg
    12541 atatgaatcg ataaggaaaa accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat
    12601 ctctgatttg gttgttttct agatagactt gtgttccagt ttttttattt tttatatgaa
    12661 aaggcttgca taaataaaaa ttcgatggta tgatgatttt gaataagaaa tggattgtta
    12721 ctgtacggca ggcaaaacca gaattcagct aagaaacagt aggcgaattc taggtttttt
    12781 tatttttctt gagagggcga agcaaaatga aaattgataa aattttgaat aacaatgtcg
    12841 tcatttcgaa aaatggattc ggggaagaag tcgtttgtat gggaaaagga ctagcttttc
    12901 aaaaaaagat tggggacgag atttcgccag aagcggtcca aaaagaattt gttttaagag
    12961 attcttttgc taaaaaccaa ttccagcagc tgatggctga tattccggca gaggagatcg
    13021 aatgggtcaa acaagtagtc gaactggctg aagataaact aaaagttgaa ttttcgccta
    13081 atatttatct gacattgacc gatcatcttc attatgcgat cacacgagca aaagaaaata
    13141 tccaactgcc taatccatta ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag
    13201 cagcgaaaga ggctcttttg ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg
    13261 aagccggttt tattgccttt cattttgtga atagccaaca aggaaacgaa gacatgcaag
    13321 tgacgatgac cgctaccaca atggtaagag atatactgag tatcatcagt aaattcttcg
    13381 gcatcgtgtt cgatgaagaa tctttgaatt atcagcgtat cattactcat ctgcagtttt
    13441 ttgcccagcg ttatctcaaa ggggaaggat ctgacgagca agatgaattt ctctacgcat
    13501 tagttcaagg aaagtatcca aaagcgtttc attgtgtcga acggatcaat gagtatcttc
    13561 gacaaacgca aaaacaggaa atggggatcg cagagcagat ttatttaacg attcatattc
    13621 aaagagtagt aagtgagaaa aaaagcatcc aatcataagg attgttactg tatggcaggc
    13681 aaaacctgga ttgaaaaaat aggttcacga atgtttgtga ctgttttttt caatctgggt
    13741 ttttattttt taaataaaaa agaaagaagg aaaaaacaat ggataatcaa gcagttggtc
    13801 gtcgtgtatg ggaagcggta ggaggacaaa agaatgtcaa aagtttagta cattgtgcta
    13861 cgcggttgcg attcagatta aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag
    13921 acccggacgt gatccaagtc gtacaaagtg gcggtcagta tcaagtagtg attggcagta
    13981 atgtagcaga tgtttatcaa tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg
    14041 gaaaagaaga gtccaaaaat attttgaatc gtctgatcga tattatctct agtatattta
    14101 caccttttct aggggcgatg gcagccgccg gggtattgaa aggattctta tcattggcaa
    14161 cggtgatggg ctggctgaca gctgatagcg gtacttatca aatattgttt gctgcagcag
    14221 atggtgtctt taccttctta cctgtcatgc ttgcttttac agcagctaaa aagttcaaag
    14281 ccaaccagtt tttagcagta gcaattgcta tggcacttgt gtatccagct atcacagcag
    14341 ttgctggtgc gggagaagct atcagtttct ttgggatccc agtgattctt tcaccatctg
    14401 gttatacttc ttctgtcata ccaatcattc tagccgtatg ggttcaaagt aaattagagc
    14461 cattcgtaaa aaaagtgatt ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt
    14521 tagtagtgat ggttccatta acatttcttg ctttaggacc aatcgggacg gttgccggta
    14581 atgctttagg cggtctcttc aactcgattt atggatttag tccaatcgta gcaggtctaa
    14641 tcatgggaag tttatggcaa gtattcgtga tgttcggcat gcattgggga ttcgtaccga
    14701 tcatgttttt gaatatcgaa caatatgggt ttgatgtatt gatgccaatg cttcttccag
    14761 ccatcttggc acaaggtggg gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac
    14821 ttcgagcatt agggatttct tctacagtga cttcgttatt tggaatcact gaaccaactg
    14881 tgtatggtgt gacattacct ttgaagaaac cattcattgc cgcttgtatt tctggaggta
    14941 ttggtggagc tattatcgga ttttccggag taaaagcttt ctccagcagt ttggtcagct
    15001 tattgacgat tccgactttc attaatacgg tggatggtgt agagtcaaat gtgacagtag
    15061 cagtaatagc tacaggaatt gcttttgttc ttgcattcgt aggtacattg attttaggat
    15121 ttgacgaaca aacacaagac aatcaattag aaaacaaaca tgcgaatgca ggagaaccga
    15181 ttacttcagc acggcatacc ttaaaaagtc ctttaacggg taaagtactg ccattgagtg
    15241 aagtaccaga ccaagttttt tcttctggcg tgatgggaaa aggtatcgct attgatccag
    15301 aagtaggaga actagttgca cctgcagacg gagagatcac gactattttt ccaactggac
    15361 atgctgtagg gatcacaacg actgacggag cggagatttt gattcatatc gggatggata
    15421 cagtagaatt aaatgggaat ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag
    15481 caggagattt attgattcgt tttgacatcg aagcaattag agcagctggc tatagcgtaa
    15541 tcactccagt tgtcattacg aatactgatg catttgccga tatacttgaa ttagaccaaa
    15601 aagagattat tgcaaacgaa gatgttctgg caattgtaaa ataaggagga aatcaggatg
    15661 caatcacgtt tttcagaaaa atttttatgg ggtggagcaa ctgctgccaa atcaattaga
    15721 aggggcttat gatcgagacg gaaaaggact atccgttgca gatgctatgc ctggaggtaa
    15781 gcagcgcttt gcgattatcg gcagtgaaga atttgactgg acgattgatc aagaaaaata
    15841 tatttacccg aatcatcgag gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt
    15901 gtttgcggaa atgggattca aatgttatcg tttttcgatt gcctggacac gaatttttcc
    15961 aaatggagac gaaggtactc ctaacgaagc gggattagaa ttttatgatc agctgattga
    16021 tgagtgctta aaatatgata ttgaacctgt gatcacgatt tctcattacg aaatgcctct
    16081 tcatttggca aaagaatacg gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg
    16141 atttgctcag acagtattag agagatacag cagtaaagtc aaatattgga tgactttcaa
    16201 tgaaatcaat tcggcctttc actttccagc tttaagtcaa ggattagtaa aaagcaatgg
    16261 agcaggagag taccaaaata ttttccaagc atggcataat caattcgttg caagtagcaa
    16321 agccgtaaaa atcggccacg aattgcgttc ggatatccaa atcggctgca tgattatcta
    16381 tgcaacaact tacagcatcg atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa
    16441 ccaagaattc aattttttct gcacggatgt ccaagtacgt ggagaatacc ctgcatacac
    16501 agcgcgtacc cataaaaaat atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga
    16561 cttccagttg ctaaaagaat atccagtgga ctacatcgga tttagttatt atatgtctac
    16621 ggcaatcaat gagactgatc cacaagcagc aacttcagaa gggaacttgc tgggaggagt
    16681 aaaaaatccg tttcttgagg caagtgaatg gggctggcag atcgatccag aagggctgag
    16741 aatcgcattg aatgaattat acaatcgtta ccagaaacca ttattcattg ttgaaaacgg
    16801 tcttggagcc attgatcata tggaagagga tggcacgatt attgatgatt atcggatcga
    16861 ttatttacgt cgtcatatcg aagcgatggc taatgcagta gcagacggtg tagatttgat
    16921 gggctatacg ccttggggat gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa
    16981 acgttacggc tttatctatg tggatctaga cgatgaagga aatgggacac tgaatcgatc
    17041 aaagaaaaaa tcttttgatt ggtataaaga agtaattgcc tcaaatggcg taaacttgta
    17101 attaaagcag ctgaatgaaa aagaacacac ttctcacatg tgatttgtgt tcttttttta
    17161 ttttttatgt gctattcttt tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca
    17221 cgtgtactat acttggaagt cgcagataaa attaaagaag atattttctc aggaaaatat
    17281 ccagtcggtt cgatgttgcc gacagaaagt gaattagaag aactgtttca agtcagcaaa
    17341 atcactgtcc gaaaggcaat cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt
    17401 ggtcgtggga cgacggttct aagcaatcgt ccttacaatc gattatccaa agcggcaagc
    17461 tttacgcaaa tcttggagaa ctcccgatat gaagtccgga aagaaacgct ggatttgcaa
    17521 ttggtccata taaagaagga agatcctttg ttttcctttt tcggtgagca agctgtttgt
    17581 tttaagcgct tatattattt gaacaatcag ccttatattt attttgttca ttatcttcca
    17641 caagaaatga tgcaattcag cagagaagaa tttggcaaag aatctttgta tcgtttgctg
    17701 acgaaaaaag gatatactat tgactcattt tctgatgatt tctcggcagt atttttaaca
    17761 gaagaagaac aaggaattct gaaaacagac gcacagatcg gcatcaaacg gatacggaaa
    17821 tcgtctacgc aaattgggga aatgattgaa tattcggagg cggtctacca tacggagctg
    17881 catccttacc atattgatta tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc
    17941 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttag
    18001 gcttattacc gcagatacac ggctgtttct ggattaccat ttattctgga taagggggtg
    18061 tggcacgatt ttcagaagga atctatgaac caatcactgt attgatttta gcttcgatgg
    18121 taaccccatt cggcttgacg atcgcttatt tcttagggaa aatcattcgt aaaaatatac
    18181 tcaatcgtca agaaatcgat acattgaaga ccgcttttcc tatggggatc tgtcagatca
    18241 cagagggatg tttccctatc gtattgaacg acttggcacg caatgtcata gcaacgggag
    18301 tcggtgggac agtaggtggc ggattaagca tgttttgggg cgcagatagc cgtattccgg
    18361 catcgggaat gtttgcagtc gctacgatga ccagaccatg ggcattcatt ggtgcattgt
    18421 tagctggttc ttttgtcaca ggaataacca ttttattatt gaaaaaacca gtagatccga
    18481 atgcagaaat catccaagaa gaaaaagagg aagaagacat atcttgggat gatttgacaa
    18541 ttagttagga gaggcagaat ggattacaag aaaaaagtac aatagatgta ccttgagaca
    18601 ggaatggtat gaaagaatca gagttagaaa gaatcgaata tacagatttt ggtttaggag
    18661 cgatcgaaac tgtccagagc atcggcaccc tccttgaaat gacgatgaag gaaaaatgag
    18721 aacctttcgt tgtcgaaagg gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa
    18781 ctttcctgta ctattccaaa aaggaaagaa agttattata cagtaaaaaa agaaattatt
    18841 cttcatgcta gagatcagta cacgttcgag cccaatatca aacactagtt ccaggcaggc
    18901 gcaacaggtg cggttatcac tgaattttct tccagcagtg acgattcaag tgatattttt
    18961 acaaatccag ctatttatcg ttaacaaaaa gatttttagg agaagctgat gaacaaaaat
    19021 gatcaaattt tgatcgttta gttcgtcggc ttttttattt agagaaacat catttatcag
    19081 tgaaacataa cacgagaaca aaatgatttt ttcgttaata aaagaaacaa gtatttgttg
    19141 tttaaaataa tacacgttat tggcatatag gacgaaatcc cagtatcttc tataagtaag
    19201 tcatttcata tcgatgaaaa cacgttattt atttttgttt tatctataaa atggctgaaa
    19261 aatgattttt ttctaaatac tatatatagt ggcgtctatc ctatatacca ctaaaaaact
    19321 acaaaatcta gtcttttatt ttaaaaaaac acgtgtagaa tgaagataga cttttgagtt
    19381 agggagtgtt tttttatgaa acttactgtt ttttgcggtt cccgttttgg taataaagaa
    19441 agttacaaat ttattgcgca aacgttagga aaatacatgg cacaagaaga aatcgaactg
    19501 gtttacggtg gttcatactc cgggattatg ggagtgatta gtaaaacagt actggaaaat
    19561 aacggaaaag tcacaggtat ctatccgaat gggctttttg aaactgaatt gcctggaaaa
    19621 gatgttacta catttattcc tacagaaacg atcgatgaaa gaaaggtatt attatttgaa
    19681 aagggagatg ctgtcctcgt tttccctgga ggtctaggta cattggagga attctcacaa
    19741 cttctttctt ggatagcaat cggtctgacg cccgacaagc caatcggaat attgaatatt
    19801 ggcggttatt acagcggctt gcaacaatta ttagaaactt ttgcaaaaga ggattttatg
    19861 gataaaaaat ggttggatca agtaatcttt tccaacaatc cattggaact ggtcaatgaa
    19921 ttacgagcag tatctattaa aaatcagtta ttattgaagg aggcaaaata atgagtcaaa
    19981 tatatttagc aggaccattt ttttcagagg agcaaatcga tcgagtaagt cgtatcgaaa
    20041 aagcgttgga ggaaaacaaa acagtcacaa gtttctactc tccacgtcat caccaagaaa
    20101 gcaattatga actattcagc gctggatggg cgcaggaagt atacgaaaaa gatatggaag
    20161 aactgacaaa tgcagaattt gttgttgcta tccttgactt tgagcatcaa acaatcgatc
    20221 caggaacggc ttacgaatta ggtgtggcaa ctatgttgaa aaaaccgatg atcattgtac
    20281 aagaagagac ggtacctacg aacttgatga ttacacaaag tctccatacg tatttaaaat
    20341 cagaccaagc tgtacgtgaa tatgattttg aaacattgcc tgttgaaact tacgtaggcg
    20401 agtacttata gaagaggaag ggttgtatgt cagtgatttt gatttcagga acgattggtg
    20461 caggtaagag cagtttgacg gatatgcttg caaaagagat tgattctaaa ccattttatg
    20521 aaaatgtcga agataacgaa gtattgccgc tcttttattc aaatcctgag cagtacgctt
    20581 ttttattgca aatcttcttt ttgaacaagc gatttttagc aatgaaaaat gctttagtga
    20641 acgatgacaa tgtgctagac cgatccatct atgaagatag cctgttgttc catctaaacg
    20701 ctgatttagg tcgggtaacg gatatcgaag tacagcagta cgatagctta ttagatacga
    20761 tgctaaacga gttagatgat gttgctccaa aaaaacggcc tgatttgatg gtccatatca
    20821 aagtttcttt ggacacgatg ctagaacgta tcaaaaaacg tgggcgtgac tatgaacagc
    20881 tagaaagcga tgagacgtta tatacctatt atgaaacgtt gaacacccgc tataatcaat
    20941 ggtatgaaga ttttgatgta tgtcctaaaa tacaagtgga tggggataaa ttcgattttg
    21001 tagaaaatcc agaagatgca aaaaaggtga tccgtcagat ccaagaaaaa ttagctgaat
    21061 tggaaggaaa gcctgctgga aaatatttta cggataacca gcatgcaaaa ggtcaagaat
    21121 taggctaaaa ataaaaaacc aaacgacggg aaagaaaaat tactacgtga tgtagaataa
    21181 gcctttc
//
LOCUS       DAGCXG010000036        20966 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGCXG010000036 DAGCXG010000000
VERSION     DAGCXG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.037"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            591..1280
                     /locus_tag="MB022_001412"
     CDS             591..1280
                     /locus_tag="MB022_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4466417.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="MB022_001413"
     CDS             1267..2475
                     /locus_tag="MB022_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4466418.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="MB022_001414"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="MB022_001414"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBT4466419.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="MB022_001415"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="MB022_001415"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBT4466420.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="MB022_001416"
     CDS             complement(6578..7273)
                     /locus_tag="MB022_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBT4466421.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="MB022_001417"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="MB022_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBT4466422.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="MB022_001418"
     CDS             complement(8472..8663)
                     /locus_tag="MB022_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBT4466423.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="MB022_001419"
     CDS             complement(8684..9574)
                     /locus_tag="MB022_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBT4466424.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="MB022_001420"
     CDS             complement(9561..10328)
                     /locus_tag="MB022_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4466425.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="MB022_001421"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="MB022_001421"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBT4466426.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="MB022_001422"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="MB022_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBT4466427.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="MB022_001423"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="MB022_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBT4466428.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="MB022_001424"
     CDS             14899..15282
                     /locus_tag="MB022_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466429.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="MB022_001425"
     CDS             complement(15450..16208)
                     /locus_tag="MB022_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBT4466430.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="MB022_001426"
     CDS             complement(16226..17044)
                     /locus_tag="MB022_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4466431.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="MB022_001427"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="MB022_001427"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBT4466432.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="MB022_001428"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="MB022_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBT4466433.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="MB022_001429"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="MB022_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBT4466434.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="MB022_001430"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="MB022_001430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBT4466435.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5995 a         4264 c         3839 g         6868 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaat
//
LOCUS       DAGCXG010000037        20362 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGCXG010000037 DAGCXG010000000
VERSION     DAGCXG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.038"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            291..1430
                     /locus_tag="MB022_001431"
     CDS             291..1430
                     /locus_tag="MB022_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBT4466436.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="MB022_001432"
     CDS             complement(1497..2072)
                     /locus_tag="MB022_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4466437.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="MB022_001433"
     CDS             complement(2077..3051)
                     /locus_tag="MB022_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBT4466438.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="MB022_001434"
     CDS             3334..4302
                     /locus_tag="MB022_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4466439.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="MB022_001435"
     CDS             4415..4693
                     /locus_tag="MB022_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4466440.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="MB022_001436"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="MB022_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBT4466441.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="MB022_001437"
     CDS             5150..5905
                     /locus_tag="MB022_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4466442.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="MB022_001438"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="MB022_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBT4466443.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="MB022_001439"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="MB022_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBT4466444.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="MB022_001440"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="MB022_001440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBT4466445.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="MB022_001441"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="MB022_001441"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBT4466446.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="MB022_001442"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="MB022_001442"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBT4466447.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="MB022_001443"
     CDS             10642..11184
                     /locus_tag="MB022_001443"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBT4466448.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="MB022_001444"
     CDS             11207..12763
                     /locus_tag="MB022_001444"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBT4466449.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="MB022_001445"
     CDS             12777..13679
                     /locus_tag="MB022_001445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBT4466450.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="MB022_001446"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="MB022_001446"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBT4466451.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="MB022_001447"
     CDS             15122..15544
                     /locus_tag="MB022_001447"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBT4466452.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="MB022_001448"
     CDS             16013..16243
                     /locus_tag="MB022_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBT4466453.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="MB022_001449"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="MB022_001449"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4466454.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="MB022_001450"
     CDS             17654..17830
                     /locus_tag="MB022_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4466455.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="MB022_001451"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="MB022_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBT4466456.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="MB022_001452"
     CDS             18325..18708
                     /locus_tag="MB022_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466457.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="MB022_001453"
     CDS             18877..19761
                     /locus_tag="MB022_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4466458.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="MB022_001454"
     CDS             19875..20111
                     /locus_tag="MB022_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBT4466459.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="MB022_001455"
     tRNA            20199..20279
                     /locus_tag="MB022_001455"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="MB022_001456"
     tRNA            20286..20357
                     /locus_tag="MB022_001456"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat ac
//
LOCUS       DAGCXG010000038        20029 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGCXG010000038 DAGCXG010000000
VERSION     DAGCXG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.039"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            29..841
                     /locus_tag="MB022_001457"
     CDS             29..841
                     /locus_tag="MB022_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBT4466460.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            838..1638
                     /locus_tag="MB022_001458"
     CDS             838..1638
                     /locus_tag="MB022_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBT4466461.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1799..3067
                     /gene="rseP"
                     /locus_tag="MB022_001459"
     CDS             1799..3067
                     /gene="rseP"
                     /locus_tag="MB022_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBT4466462.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVIKKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3135..4844
                     /locus_tag="MB022_001460"
     CDS             3135..4844
                     /locus_tag="MB022_001460"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBT4466463.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDYVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5056..9408
                     /locus_tag="MB022_001461"
     CDS             5056..9408
                     /locus_tag="MB022_001461"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBT4466464.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAERNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYAKYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVTQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9552..10025
                     /gene="rimP"
                     /locus_tag="MB022_001462"
     CDS             9552..10025
                     /gene="rimP"
                     /locus_tag="MB022_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBT4466465.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFIKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10049..11224
                     /gene="nusA"
                     /locus_tag="MB022_001463"
     CDS             10049..11224
                     /gene="nusA"
                     /locus_tag="MB022_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBT4466466.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11246..11539
                     /locus_tag="MB022_001464"
     CDS             11246..11539
                     /locus_tag="MB022_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBT4466467.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11536..11847
                     /locus_tag="MB022_001465"
     CDS             11536..11847
                     /locus_tag="MB022_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBT4466468.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11860..14166
                     /gene="infB"
                     /locus_tag="MB022_001466"
     CDS             11860..14166
                     /gene="infB"
                     /locus_tag="MB022_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBT4466469.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14193..14540
                     /gene="rbfA"
                     /locus_tag="MB022_001467"
     CDS             14193..14540
                     /gene="rbfA"
                     /locus_tag="MB022_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBT4466470.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14648..14869)
                     /locus_tag="MB022_001468"
     CDS             complement(14648..14869)
                     /locus_tag="MB022_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466471.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15317..17680
                     /locus_tag="MB022_001469"
     CDS             15317..17680
                     /locus_tag="MB022_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBT4466472.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWLKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18049..18972
                     /gene="truB"
                     /locus_tag="MB022_001470"
     CDS             18049..18972
                     /gene="truB"
                     /locus_tag="MB022_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBT4466473.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEMTLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRIDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18976..19917
                     /gene="ribF"
                     /locus_tag="MB022_001471"
     CDS             18976..19917
                     /gene="ribF"
                     /locus_tag="MB022_001471"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBT4466474.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6873 a         3518 c         4390 g         5248 t
ORIGIN      
        1 gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata
       61 tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc agcacattgc
      121 catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg
      181 ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt
      241 gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt
      301 aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag aattgattaa
      361 agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac atacgcaaga
      421 tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat tgaattttgc
      481 tttgaattat ggaagtagag cagagatcgt gactgctgtc caagaaattg ccgaagaagt
      541 agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta agcatttaat
      601 gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta caagtggaga
      661 agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt attttacaaa
      721 agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa
      781 tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg
      841 aaacaacgtg tgatcacagc agctgtggcg ctagtcttgt ttattccgat tatatggatc
      901 ggaggcatag cggttgagct tacagcagca atcttagcgg tagtaggagt ttacgagctg
      961 ttccggatga aaggattgac actcttaagt tttgaaggag ttttatccgc cgttggtgca
     1021 gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa tgtaagtaca
     1081 tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat ttctaaaaat
     1141 acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt gggaataggt
     1201 ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt tggtctattt
     1261 attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg tcagcataag
     1321 ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg tatcggtagt
     1381 gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact attcaatcat
     1441 ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca atttggtgat
     1501 ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa tatcttacca
     1561 ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat
     1621 ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat aaaagacgca
     1681 ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat ggtaaaatta
     1741 tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat
     1801 gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc atgagtttgg
     1861 tcatttcttc tttgcgaaac gatcaggaat cctcgttcgc gaatttgcta tcggtatggg
     1921 tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc gcctattacc
     1981 tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa tggcaccagg
     2041 aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa atttaagtaa
     2101 gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt tagaagatga
     2161 gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct accctgttga
     2221 tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc caaaagacgt
     2281 acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg gacccatgaa
     2341 taactttatt ttggcaatcg tgttattcat cattctggca tttatgcaag gtggtgttca
     2401 agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg cagaagcagg
     2461 cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt catggaatga
     2521 tctgacaaca gtcattaaga aaaatccagg taaaacatta gactttaaga tcgaacgaga
     2581 aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt
     2641 tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga tcattggagg
     2701 gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat cactatttac
     2761 aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt cttcagaagc
     2821 agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa tgaatctcgg
     2881 aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct tgaatatttt
     2941 tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga cattagctgg
     3001 ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc aacgattctt
     3061 tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt tgaggaaata
     3121 aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc gaagttccaa
     3181 atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc cgccaagtat
     3241 cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa ctgaaacgca
     3301 ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct gccatcttgc
     3361 cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt
     3421 tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa acttttactg
     3481 atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt tatcaaatcc
     3541 aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga agagagttta
     3601 tcatgaaaga tgcatattct ttccatgcaa cggaagaaag tttggacgag acatataaag
     3661 attacgaaaa agcctatacc gaagtattca agcgttgtgg cttagaattt cgctcgatca
     3721 tcggagacgg cggcgcaatg ggcgggaaag actcaaaaga attcatggcg atctcagaaa
     3781 tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac ttggagatgg
     3841 ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag
     3901 ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt gaacctcaaa
     3961 aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt ttggtacgcg
     4021 gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac tttttagaag
     4081 aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct gtcggaccag
     4141 tcggtgtttc agattacgta aaagtatatg cagaccgtta tgtccaagat ttagcgaatg
     4201 ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca gaacgcgatt
     4261 tcactgtact ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct tcaccagaca
     4321 acaatggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatcttc aaaatcggca
     4381 ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga gaaaaatttg
     4441 tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac
     4501 attcagatga acaaggaatc aattggccaa aaggaattgc gccatttgat ctgcatatcg
     4561 tccaaatgaa tctaaaagac gaataccaaa caaacctgac gaatgaacta gaagaagcga
     4621 tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca ggagtgaaat
     4681 tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg
     4741 atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag
     4801 aattggcgaa tactttacca atcctcttga accaaggcga ataataagcg ttaaaacaca
     4861 gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct tttaaaattg
     4921 gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa aaacgagtat
     4981 actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt ttctcatcag
     5041 aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt ggaacaaatc
     5101 caactagagg aagcagaaag aaatcatcca ctgcttactg ctggtgaaat ggatagtgta
     5161 gtggtccatc gtaaatcgcg tttatgggaa tttacgctgc atttttccaa aattttgcct
     5221 attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga tattgcacaa
     5281 gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt gcaagattac
     5341 tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca agtattgaaa
     5401 acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag tacaggagct
     5461 atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt cagttacggt
     5521 ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg tgtattgaaa
     5581 ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc agctgaaagt
     5641 ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga
     5701 cccatccagc taggacgaaa tattccagcc gatgaaccaa cgacaccaat gatcagtatc
     5761 gttgaagaag aacgaagagt cacacttgag gggtacgtat tcgacaaaga agtcagagaa
     5821 ctacgttcaa aacgcaagat cttgactttg aaaatcacag attatacttc atcatttatc
     5881 gtcaaaaaat tctcgaacgg cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt
     5941 agctggttaa aggtacgagg aagtattcaa gaagatacgt ttgtccgtga tttggtcatg
     6001 aatgcgcaag atatcataga agtcaaacat acacctcgca aagattatgc tcctgaaggg
     6061 gaaaaacgag tagagcttca tgttcatagc aatatgagta cgatggatgc aacaaacagc
     6121 atctctgatc ttgtggcaca agcagggaaa tggggacacc gagctatcgc cattacagac
     6181 cacggtggtg cgcaagcctt tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa
     6241 atcttatacg gcgtggaagc gaatgtcgta gatgatggtg tcccaattgc ttataatgat
     6301 gcacatgaag cattgagtga agccacatat gtcgtatttg acgtggaaac gacaggattg
     6361 tctgcagtat atgacacgat catcgagcta gccgcagtca aaatgtataa aggaaatgtg
     6421 atcgaaagct ttgatgaatt tatcgatcca ggacatcctt tgtcacggac aacggtagat
     6481 ctgactggta tcacggatga aatggttcga ggatcaaagt cggaagaaga agtattgcgg
     6541 atgttccttg aattttcaaa agatacaatt ttggtagccc acaacgcggc ttttgatatg
     6601 gggttcttga atactagcta cgcaaagtat ggtattcctg aagcggccaa tccagtcatt
     6661 gatacgttgg aattagctag atatctgtat cctcaattca aacgtttcgg attaggtgtg
     6721 ttatctaaaa aatttggtgt cagcttggaa cagcatcacc gagcaatcta tgatgctgaa
     6781 gcaactggtc acttagcatg gatctttgtg aaagaggcaa tggacaatca taacatgctt
     6841 tatcatgatc agctaaatga gcatatcgga gaaggagatt cttacaaaag ggctcgacct
     6901 tttcatgtaa cgattttagc aaaaaatcag gcaggactga aagatttgtt caaactgatt
     6961 tcaatgtcga atgtcgaata cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa
     7021 aagatgcgtg aaaatctatt gatcggctca gcttgtgata agggagaaat attcgaagcc
     7081 atgatgcaaa aaggggtaga agaggcaaga aaccgagcaa aattctacga ttatattgaa
     7141 gttatgccaa aagcggtcta tgcgcctttg atcgagcaag aattggtaaa aaatgaacat
     7201 gatttggaag aaattatcca aaatttggta gaaatcggaa aaagtttgga taaaatcgtt
     7261 gttgcaacag gtaatgtaca ttatctgaac gaagaagatg ccatttatcg aaaaattttg
     7321 atcaattcta tgggcggagc gaatccgttg aatcgtcata gtttaccgga tgtccatttc
     7381 cgaacaaccg atgagatgct aactgcattt cactttttag gggaagaaac agcgaaagag
     7441 atcgtagtag aaaatacgaa taaaatcgca gatatctgcg aagaagtgat tccagtcaaa
     7501 gatgaacttt atacaccgaa gattccaggt tcagaagatg aaatatcaga attgagttac
     7561 accaaagcaa aacaaatgta cggagatccg ttacctgaga tcattcaaaa acgattagaa
     7621 aaagagttga attctattaa tggtaatggc ttctcggtta tttatctgat cgcccaaaaa
     7681 ttagtgcata aaagtaatga agatggctat ctagtaggtt cacgtggctc tgttggctct
     7741 agctttgttg cgacgatgac aggaatcact gaagttaatc cattagcccc tcattattat
     7801 tgtccagagt gccaatattc cgagtttttt gaagatggca cgtatggttc tggatttgac
     7861 atgccagaaa aacaatgtcc aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt
     7921 ccatttgaga catttttagg attccatgga gacaaagtgc ccgatattga tttgaacttc
     7981 tcaggtgatt atcaagcaga agcccataac tatacgaaag tattgtttgg cgaagactat
     8041 gtttaccgtg ccggaacgat tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa
     8101 ggatacgaaa gggacaataa tctgcagttt cgaagtgcag aagtcgacag attagcaaaa
     8161 ggcgctactg gagtcaaacg gacaaccggc cagcatccag gggggatcat cgttattccc
     8221 gattatatgg atgtctatga ttttacgccg atccaatatc cagcagacga tcaaaattca
     8281 gaatggaaaa cgactcattt tgatttccac tcgattcacg ataatgtatt gaaattggat
     8341 attctagggc atgatgaccc aactgttatt cggatgcttc aagatttatc agggatcgat
     8401 ccacaaacga tccctacgga tgacccggaa gtgatgcgga tatttgccgg tcctgaagta
     8461 cttggagtaa ctcaggaaca aatttattcc aaaacaggga cattaggtat tccagaattc
     8521 ggcactcgtt ttgttcgagg aatgttagaa gaaacgcatc ctacaacttt tgcagaacta
     8581 ctgcaaattt ccggactatc tcatggtaca gacgtatggt taggaaatgc ggaagaattg
     8641 attcgacgag gagatgcgac actggcggaa gtaatcggct gtcgtgatga tattatggtt
     8701 tatttgatcc atgctggatt agatagcggg atggcattta aaatcatgga aactgttcgt
     8761 aaaggacaat ggaacaagat ccctgatgaa ttgcgagaaa cctatctatc ggctatgaaa
     8821 gaaaataatg tgcccgactg gtatatcgat tcttgttcaa aaatcaaata tatgtttccg
     8881 aaagcccatg ctgcagctta cgtattgatg gctcttcgtg tcgcttattt caaggtttat
     8941 ttccctattt tgtattactg tgcatacttt tctgtacgtg cagacgactt tgatctagtc
     9001 tccatgtgca aaggaaaaga tgctgtgaaa caagcaatga aagaaatcac ggacaaagga
     9061 ttagatgctt ccgtaaaaga aaagaatcaa ctgacagtgc tggagttagc caatgaaatg
     9121 ctggaacgag gattcaaatt tggaatgatt gatttgtata aatcggatgc cgttaacttt
     9181 gttattgaag gtgatacgtt gatcgcacca tttagagctg tcccaagttt gggaaccaac
     9241 gtggcaaaac aaatcgttga agcaagaaaa gacggtccat ttttatccaa agaagatctt
     9301 gccacccgcg gtaaagtttc aaaaacattg atcgaatata tgaacgacaa tggcgtattg
     9361 aaagatctac cagatgaaaa tcaattatcg ttattcgata tgctataagc atctgttttc
     9421 ctgtctgctt gtaattttaa gcagagtatg gtatagttct tataggtaat gatactaact
     9481 gtgagtgagc ggatttttcg ctcactcttt ttaatggaat tacgcactta atttttaagg
     9541 aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag
     9601 atgaacaaaa ttttgaactt gtcgaagtcg aatttataaa agaaggaaaa agctggttct
     9661 tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg
     9721 agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc
     9781 tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg
     9841 cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg
     9901 aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac
     9961 gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat
    10021 tttaatcacg gagaggaatc agaaaaaaat gagtaaagaa atgttaaatg cgttagacgc
    10081 tttagaagct gaaaaaggga tctcaaaaga aatcgtgatc gatgctttag aagcagcatt
    10141 ggtttctgca tacaaacgtc actatggcca agcacaaaac gtcgaagtgg aatttgatca
    10201 gaaaaaagga aagatccatg tctatgcggt aaaagaagta acggaagaag tgatggattc
    10261 acagttggaa gtctcattga aagatgcact tttaatcaat ccagcatatg agatcggtga
    10321 tacgatccgt tttgaagtaa caccaaaaga ttttggacgg atcgctgcgc aaacggctaa
    10381 acaagttatt ttacagcgtg tacgtgaagc agaaagaacg attatttata acgaattcag
    10441 tgcttatgaa aaagacatca tgcaaggaat cgtcgaacgc caggataaac gttatattta
    10501 tgtcaatctt ggtaaaattg aggcagttct ttctaaacaa gatcaaatgc caaatgaatt
    10561 ttatcaacca catgatcgaa tcaaagttta tgtttcccga gtagaaaata cgtctaaagg
    10621 tccgcaagta tttgttagcc gcagccatcc agacctattg cgtcgcttat ttgaacaaga
    10681 agtaccagaa gtctatgatg gaatcgttga aattgtcagc atcgcccgtg aagctggcga
    10741 tcgttcaaaa gtggcagttc gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt
    10801 cgggccaaag ggacaacgag ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga
    10861 tatcgtcgaa tggaatgaag atccagctgt attcatcgca aatgcattga atcctgcaca
    10921 agtcatggat gtgatcttcg atgagacaaa tccaaaggcc tgcacagttg tcgttccaga
    10981 ctaccaatta tcgctagcaa tcggtaaacg cggacaaaat gcacgtttag ctgctaaatt
    11041 gactaaccat aaaatcgata tcaaatcaga atctgacatg acggaatttt atgaaaatca
    11101 agaacaacaa aaagaaacag aagaactgca tgatgaagca atcgttcagt cagatttaac
    11161 agacgatgaa tatgaaacaa ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc
    11221 ttaactttag taaggaggca aagagatgaa acaaaggaaa attcctttac gcaaatccgt
    11281 ggtttcaggt gaaatgaaac ctaaaaaaga gatggtccga atcacccgtt caaaagaagg
    11341 ggaagtatcg atcgatccaa ctggaaaaat gcctggacga ggagcgtatg tctcacttga
    11401 gccggaagaa gtacaacaag cttgggataa aaaaattctc gatcgagtac tcgaaacaac
    11461 attaacagat gagttttatc aagaacttct tgactatgtc actcaccaaa aagcacgtaa
    11521 agagttattt ggaaaatgaa tacagttaat cggcaaaaag ccatgaatct catcggtcta
    11581 gcgatgcgcg cgggaaaact aatcacgggt gaagaactga ccattgctga aatccgcaaa
    11641 caaaaagcaa aattggtgtt tgtggcaacc gatgcaagtg agaatacaag aaaaaagata
    11701 aaagataaga gttcatatta tgaagttcct tgctttgagc tattttctga agcagaaatc
    11761 acgcagatga tcggaaaacc tcgcaaggta ttcggaataa tggatgccgg atttgcgaag
    11821 aagaccaagg agctaattga aggttaggaa ggtgattgca tgggcaacaa aagaatctat
    11881 gaattagcaa aagaactcaa aaaatctagt aaagacgtcg tggaaaaagc acaacaactt
    11941 ggcatcgatg tgaaaaacca tatgggtgcg attactagca gtgatgagaa gaaattgcaa
    12001 caagcgttca aaaacccaag cacgaaacaa caagctgcac aacaagcaag cagaaaacca
    12061 gccaatcaac agccgaacga tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat
    12121 aaaaataacc gcagttatca agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa
    12181 aaaccaacaa caaaccaaaa taatacggga aataccagca gccaaaatcg tcaaggaacg
    12241 agccaaggcg gcaatcaaaa ccgtactaac aataacaaca aaccaaacaa taacaatcgc
    12301 ggcaaattta ataataataa ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag
    12361 caacaatcaa ctaaaccagc tgtaccacca cgcaaattcc gtgaattgcc agatgtatta
    12421 gagtatactg aaggtatgaa cgtagcagat attgcgaaaa aaattcatcg tgagccagca
    12481 gaaatcatta aaaaattatt tatgcttggt gtcatggtca atcaaaacca agcactggat
    12541 aaagatacga ttgaactgtt agcgacggac tatggcatgg aaccacaaga aaaagtccaa
    12601 gttgacattg ctgatatcga taaattcttc gaggcagaag aagtcgatcc agataaatta
    12661 gtctcacgtc cgccagtagt aacgattatg ggacacgtag accacgggaa aacaacctta
    12721 ttggatacat tgcgccattc acgtgtaaca agcggcgaag ccggcggaat cacacagcat
    12781 atcggtgcct accagatcga tattgatggt aaaccaatca ctttcttgga tacaccagga
    12841 catgcggcct ttacaagtat gcgtgcacgt ggagcaagta tcacagatat cacgatctta
    12901 gttgttgctg cagatgatgg cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa
    12961 gctgcaggcg ttccaatcat cgtcgctgta aataaaattg ataaaccagg tgcaaatcct
    13021 cagcatgtta tgcaagaatt aagtgagtat gaattaattc ctgaagcatg gggtggagaa
    13081 acgatttttg ttgagatctc agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg
    13141 atcctgttag tcgcagaagt agaagattta aaagcagatc caacacaacg cgcaatcgga
    13201 acagtgatcg aagcacgtct tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa
    13261 gaaggaacac ttcacgttgg tgacccaatc gttgtcggta atacttacgg acgtgtccgt
    13321 gtaatgacaa acgatcttgg ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa
    13381 atcactggct tgaatgatgt acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa
    13441 aagacagcac gtgcagcggg tgaagaacgc gcaaaacgtg cattattgga aaaccgtgct
    13501 gccacaagcc gtgtgacttt ggacaactta tttgaaagcc tgaaagaagg cgaattgaaa
    13561 gaagttaacg taatcatcaa agcagatgta caaggttcag cagaagcact ggctgcatca
    13621 ttgaagaaga ttgatgtgga aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca
    13681 atcaatgaaa gtgatgtaac cttagcagca gccagcaacg ctattatcat cggtttcaac
    13741 gttcgtccta caccgcaggc aaaagtgcaa gctgatactg aagaagtcga tattcgtctt
    13801 caccgtatca tctataaagc catcgaagaa atcgaaactg cgatgaaagg gatgctagac
    13861 cctgaatttg aagaaaaaat caccggtcaa atgatcgttc gtgaaacctt caaagtttct
    13921 aaagttggta cgattgcagg tgcctatgta acagaaggat atattcgtag agatagcggt
    13981 gtccgtgtaa tccgtgacgg aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga
    14041 ttcaaagatg acgtgaaaga agtcaaaatg ggttatgaat gtggtgcgat gattgaaaaa
    14101 ttcaatgaca tcaaagtaga tgacgtaatt gaaggattta tcatggaaga agtcaaaaat
    14161 gactaaaaac tgataaaagg aggccaatca ttatggctaa ctaccgtgat cgccgagtcg
    14221 gtcaagaaat cttaaaagaa gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg
    14281 tggagaacgt aacgatcaca gatgtgaaag ttacggggga cttacagcaa gcaacgatct
    14341 attacagcat tttatctgac ttagcatcag ataagaaaaa agcacaagat ggattgaata
    14401 aagcatcagg attgattcgt cgagagttag ggcacaatct aagtatttat aagacacctg
    14461 aattgttctt tgaacttgat gaatcagttg tctatgggaa ccatatcgat gaattgcttc
    14521 gcgacttgaa caaagactaa tcaaaaaatc ccgcaaatcc tgtgaaatca ggggttgcgg
    14581 gatttttttg ttttcattaa gacgtattac ctaagagttc catgacttta gtctggtagc
    14641 gtgtcagcta accaaaaaag agaaaaagaa aagtaaaaaa ttcgactttc ttttttctat
    14701 agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt
    14761 aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac
    14821 cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat
    14881 tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt
    14941 agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt
    15001 tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccaacaat
    15061 gtgttaactg gcctagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc
    15121 ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag
    15181 gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg
    15241 aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg
    15301 aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg
    15361 gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata accccttact
    15421 gagacgtaca ctgaaaccag aagatgtcaa aaaacatcca atcggtcatt ggggaacgat
    15481 tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa
    15541 catgttttat atcgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt
    15601 agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa
    15661 attattcaag cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc
    15721 aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt
    15781 attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg
    15841 tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt
    15901 tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa
    15961 atcagatgga gaattagcaa attactttaa cggacttggc tgggagcctt tctttattga
    16021 aggaaatgat ccagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga
    16081 gaaaatcaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc
    16141 aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga
    16201 cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga
    16261 acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt
    16321 atttgatgca caaggacgta ttttagaaga aatcagagaa attgcgccta ctggtgacca
    16381 aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc
    16441 agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat
    16501 gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat
    16561 atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg
    16621 ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt
    16681 catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac
    16741 tggacggcat ggtttttttg caagttacga atcattcttg cgagtggttg actctatgtt
    16801 gactcagcac ttcaaatgga tacggaaatc acatgacctt tcttggctaa aagattatcc
    16861 ttcgctgaat ctcattgcaa gctcaacagt cttccagcaa gatcacaatg gatatagcca
    16921 ccaagatcca ggaatattga cacatttggc agaaaagaag gcggagttca tccgtgaata
    16981 tctgcctgcg gatgcaaata cattgctcgc agtcatggat aaagcattcc gctcgtcaga
    17041 aaaaatcaat ttgatcatct cttccaaaca tccgcgtgct caattttact cagcagaaga
    17101 agcagccgtc ttagtgaatg aaggcttgaa aatcattgat tgggcttcga cagcaaaaga
    17161 ggaggaacct gaattggtca ttgctgctgc aggaacggaa tctaacttag aagcattagc
    17221 agctgtaact ttgctgctgg aagaattccc aaaactaaaa attcggttca tcaatgtcgt
    17281 agatttattg aaattacgtc atccaagtca agatccgcgt gggttgagcg atgaagaatt
    17341 tgatcaatat tttacaaaag acaaaccgat cttatttgct ttccacggtt atgaaacatt
    17401 ggtccgcacg attttctttg atcgtcataa ccatcattta atgattcatg gctacaaaga
    17461 aaatggagat atcacgacac catttgacat gcgtgtagta aatgaacttg atcgttatca
    17521 tttggcaaaa gacgcagctt taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa
    17581 aatggatcag aaattacaag aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc
    17641 agaagtattg gattggaaat ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc
    17701 aaaacagaat tttttacatt gattaagaaa ctgaaaaaat aaataaaaaa aacggaaaaa
    17761 agactggaag aatggacagt cttttttttc gtttttggga agtgaaaacc aaactattta
    17821 cctgtaagcc acccataaat ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc
    17881 cctcctttaa aaaaagcata acatgatttc tatttacgtg agcatgaatt ttgttacgtt
    17941 tttattgccg aaaaataaaa accgttcatt atgatgtcaa attcgtcagt aattgatgca
    18001 atgtgttata ataattcagt taaatcaaaa atgaaatgga gaaaagacat ggacgggtta
    18061 ttacctttat ggaaagaacg cggaatgaca agtcatgatt gcgtattcaa attaagaaaa
    18121 atcttacata cgaaaaaaat cggacatggg ggtacgcttg acccagacgt agacggtgtg
    18181 ttgcccattt gtattggaaa agccacaaaa gttatcgaat atttgacaga ttctgggaaa
    18241 atctatgtag gcgagatgac tctaggtttc agtacaacga cggaagatcg atccggagaa
    18301 cgggtagaaa caaaaccagt agatatgtct ttatctgaag aaaaaataga tgaagtgatg
    18361 gcttcattta ttggtgagat cacccagata ccaccaatgt actcagcggt aaaagtcaac
    18421 ggtaagcggt tatatgaata tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag
    18481 gctcagattt accgttttat ccggacaagt gaattgacaa gagacgaagt gacaggaacc
    18541 atttcttggc gcttcgaagt agaatgcggg aaaggaacct atgtccgcac attagctgta
    18601 gatacaggca agaaacttgg ttaccctgcg cacatgtctg atctgacccg aacagcaagt
    18661 gccggtatgt cccagaagga tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat
    18721 gggacaatcg atgattgttt gctgccaata gaaaaaggag tggaacggtt caaacgaata
    18781 gatattactg atgaagtatg gcagaaagta aaaaacggta tgagacttaa ttatactgta
    18841 tttgcgctcc cagaaatgcc gtcagaagaa attgctttgt tttataaagg caaagttgtc
    18901 agtatctatc agccaaaccc gaaagaaaaa aacaaattaa aacctagtaa agtattgaga
    18961 aatgaggaat aacgaatgaa tatcatcaag atcagacatc cttaccaatc agaacaaatt
    19021 ccagaagacg atgtcgtttt agtactggga ttttttgacg gcgtgcatat tggacaccag
    19081 aaagtcataa agacaggtat ggaaattgca caaaaagaag gattgaaatt agcattgatg
    19141 actttcaatc agcatccatc gattgtcttt aaaaagatcg atcctagcag cgtgaaatat
    19201 ttgactagcc ttaagcaaaa agaagaaaaa atggccgctt tgggtataga ttatttatat
    19261 gaagttgatt ttacttcttc ttttgctcat ttagcgccgc aggaatttgt tgatcaatac
    19321 atcgtagggt tacatgcaaa atatgctgtt tccggttttg attatacgta tggaccaaaa
    19381 gacattgcag atgttgctca ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca
    19441 gtttctaaag aagaagaaca aggtgaaaaa atcagctcta cccgtattcg aggattacta
    19501 gacagcggac aagtaaaaga agcagcaaaa ctattaggtt gcacatatga ggtagaaggg
    19561 gtagttgtcc atggcgatgc acgaggcaga ctactaggat tccctacagc aaatgtcaaa
    19621 gtaaagagca cagttcattt gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg
    19681 gaaacttggt atccagccat gggttcaatt ggacataatg atacattcgg tgaaggacgg
    19741 gaactgactg tagagttgta cattttggac tttaatcaag atatctatgg tgaacatgtc
    19801 gcagtacgct ggaatcaatt tttaagagat caagtgaaat tttctagtgt agaggaattg
    19861 attgaacaac tcaaagagga tgaacaagca acagctgaat atttttctca gcaataaaac
    19921 attatgcata aaatctcttg tttatgtata actagcaatt acaataaaat gggtgataaa
    19981 taagcccagg aaaggggctg tgacaaaagt cttgtcacag ccttttttt
//
LOCUS       DAGCXG010000039        19480 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGCXG010000039 DAGCXG010000000
VERSION     DAGCXG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.040"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..287
                     /locus_tag="MB022_001472"
     CDS             <1..287
                     /locus_tag="MB022_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466475.1"
                     /translation="EISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMVANIKN
                     SKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            547..891
                     /locus_tag="MB022_001473"
     CDS             547..891
                     /locus_tag="MB022_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4466476.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            896..1177
                     /locus_tag="MB022_001474"
     CDS             896..1177
                     /locus_tag="MB022_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466477.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1280..1594
                     /locus_tag="MB022_001475"
     CDS             1280..1594
                     /locus_tag="MB022_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBT4466478.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1572..3266
                     /locus_tag="MB022_001476"
     CDS             1572..3266
                     /locus_tag="MB022_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4466479.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3286..4464
                     /locus_tag="MB022_001477"
     CDS             3286..4464
                     /locus_tag="MB022_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4466480.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4427..5113
                     /locus_tag="MB022_001478"
     CDS             4427..5113
                     /locus_tag="MB022_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBT4466481.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSAGTEAKKEQSVEEILNDVEEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            5113..6273
                     /locus_tag="MB022_001479"
     CDS             5113..6273
                     /locus_tag="MB022_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4466482.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVNNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKTAEPITALDAATMGGLMARLSKFNIEGVDDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6283..7158
                     /locus_tag="MB022_001480"
     CDS             6283..7158
                     /locus_tag="MB022_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466483.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGARWIINLPKANYWKKVYLDSNRLYRYLRGQKTNVQIWTAEMWAQLWNMMYFNIGP
                     KVHEELNFCFATDPIEKFKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYAKAIKAVVR"
     gene            7155..7466
                     /locus_tag="MB022_001481"
     CDS             7155..7466
                     /locus_tag="MB022_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466484.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPDSLEYGLLIERCRYDWNRALGEFEQNFASELLGFIQHYALQEYIAGDGNG
                     E"
     gene            7456..7809
                     /locus_tag="MB022_001482"
     CDS             7456..7809
                     /locus_tag="MB022_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4466485.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGE"
     gene            7799..8200
                     /locus_tag="MB022_001483"
     CDS             7799..8200
                     /locus_tag="MB022_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466486.1"
                     /translation="MVNDNSKEKERLNKQISAIKTSLEEHFKLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8193..8597
                     /locus_tag="MB022_001484"
     CDS             8193..8597
                     /locus_tag="MB022_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466487.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8609..9217
                     /locus_tag="MB022_001485"
     CDS             8609..9217
                     /locus_tag="MB022_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4466488.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9237..9599
                     /locus_tag="MB022_001486"
     CDS             9237..9599
                     /locus_tag="MB022_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466489.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9801..13220
                     /locus_tag="MB022_001487"
     CDS             9801..13220
                     /locus_tag="MB022_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4466490.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13271..14008
                     /locus_tag="MB022_001488"
     CDS             13271..14008
                     /locus_tag="MB022_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4466491.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            14018..16309
                     /locus_tag="MB022_001489"
     CDS             14018..16309
                     /locus_tag="MB022_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4466492.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16333..18423
                     /locus_tag="MB022_001490"
     CDS             16333..18423
                     /locus_tag="MB022_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466493.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            18440..18589
                     /locus_tag="MB022_001491"
     CDS             18440..18589
                     /locus_tag="MB022_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466494.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            18586..19032
                     /locus_tag="MB022_001492"
     CDS             18586..19032
                     /locus_tag="MB022_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466495.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19034..19171
                     /locus_tag="MB022_001493"
     CDS             19034..19171
                     /locus_tag="MB022_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBT4466496.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19209..>19480
                     /locus_tag="MB022_001494"
     CDS             19209..>19480
                     /locus_tag="MB022_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466497.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT         6952 a         2899 c         4071 g         5558 t
ORIGIN      
        1 acgaaatatc ttatctcaat ccaacagacc ttattaatca agaattccag aatattggat
       61 acttagaaaa aggtctaaaa ataccaattg taaatgaaag tttagttact gcaatggtag
      121 ctaacataaa gaacagcaag aaattagaaa agagatataa aaaatgtaaa aaattgaaat
      181 tttccttttt attcttagaa tataatggca aagaatatta tcaagatatt tatgttttgc
      241 gaggctcgaa cttatataaa gacattaaaa ataagatttc agtatgagat tttttaaaat
      301 tcgtagatca ctctttgagt ggtcttttta ttttgcgtaa aggaggcacg acgatgtata
      361 gaccacaata cttagaacag aagcatgaag taatcactgt gcaaaacggt aacggtgaga
      421 tagtacgaaa gtatagaaga ccaataaaga gcgatacata taaacggaag gaaagcaatg
      481 aagttattcc attgtatggc aaaagaatag ctaagtatta aataagattg cgaaaggaga
      541 tgggacatga ccgaggaatt ctatagatgg ttattacagt tgataagaga agatcgtttg
      601 gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat gaaacgagat
      661 cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga gaacgttcat
      721 catataaagg aagtcaagga tagacctgac ttagctttag atttagataa tcttatttgt
      781 ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcgtt agataaacaa
      841 gagaagaaga tagaaagctt cgctaacttc gatgcaagtg aaaggtggta agtgcatgat
      901 catcaatgac aatggcagag agtatgatac agaaaagatt gaagagtatt catcttatac
      961 tcagggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg atctgttatc
     1021 agataactgt tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga ataaagataa
     1081 taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta attattacat
     1141 tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa taaatcgcaa
     1201 attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt tcgaacttta
     1261 tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa
     1321 attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg agccattctt
     1381 agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg aagaagatat
     1441 atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag cgttgcttgt
     1501 ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc ctgaattggt
     1561 aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta
     1621 tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat tatctagaag
     1681 aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag gattcaatca
     1741 atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt ctacaatgtt
     1801 ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga tatctttcat
     1861 taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc tttctactaa
     1921 ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat gaaagccaag
     1981 caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat ttagataaaa
     2041 aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc aaacttcgtt
     2101 ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg
     2161 aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt ggtggtaaaa
     2221 ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg
     2281 atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt gacaaggatg
     2341 gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat aatgaagttg
     2401 atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca gatttaaaac
     2461 ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga cttacgttca
     2521 tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct tcatatgatg
     2581 atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag
     2641 tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa tacgataaag
     2701 atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta caaaacatca
     2761 aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt catggaaaaa
     2821 atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa tattatatta
     2881 aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta gaagaagcag
     2941 gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta aaagatctgg
     3001 ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt atgcgttggt
     3061 attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat gaaaaaatag
     3121 aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg aattttttag
     3181 atgatattta tgattctgct cctgtaacag tcacaaatag ctcagtagaa aatacaggaa
     3241 ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg gatttttcaa
     3301 aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata cttttgtaaa
     3361 ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga tttgattgca
     3421 aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa aaagaatgat
     3481 ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga atttagaaaa
     3541 aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc gtctaataat
     3601 tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt gaaagatgat
     3661 gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct agaaagtgat
     3721 gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc gtattatcaa
     3781 gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa cgctcgtaga
     3841 tacgtactga aagggaattt attccgaccg caagacaata caacacaaga tcaaatcaat
     3901 aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg tgcggtattt
     3961 caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag caattcaaga
     4021 gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt tcacgttccg
     4081 aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc ttttttaatg
     4141 ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag tctctatgaa
     4201 gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat caagattact
     4261 agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt atttacaatc
     4321 aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc agatgaaata
     4381 tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa
     4441 aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc agggatttat
     4501 tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata ttttggatag
     4561 tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag acgtatttca
     4621 agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg ttgtgattaa
     4681 cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta taaaaatcta
     4741 caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga
     4801 cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg cttcatataa
     4861 aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag aatcgtttat
     4921 ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg cagaaaatag
     4981 cgcaggtacc gaagctaaga aagaacaaag cgtcgaagaa attttgaatg acgttgaaga
     5041 aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat ttggacaagg
     5101 agatgcaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag cgaccaattg
     5161 atgaaagctt ttaaagatgg taacgaagag tcttttagcg cagctatggt aagtttatct
     5221 aaggaaatac aggataaaat tttagaagaa gcaacagcaa aaaatcaaga tcaattagta
     5281 ttaatgaacc gtgggcagcg tgtactaact acacaagaaa caaaattcta taacgaagta
     5341 gtgaataatg aaggttttgc aggagttgaa gaattagtac cagctactgt atttgaacgc
     5401 gtatttgaag atctagaaca atctcatcca ctattgcaaa aaattacttt tgttaacaca
     5461 actggtgtaa cagaatggat cgtgtcacgt ggagtcaatc cagcatggtg gggcaaactt
     5521 tgcgaagctg ttaaaaaagt tttagataat ggctttgacg taattaacat gaagcagttc
     5581 aagctatcag gttatattcc tgtatgtaag gcaatgcttg acttaggtcc agtatggtta
     5641 gatcgttatg ttcgtactgt tttagttgaa tcattgagaa ttgcattaga acaagcaatt
     5701 gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gcgacatgag caaacaaact
     5761 agcggagaat atgctgaaaa aacagcagaa cctattacag ctttagatgc tgcaactatg
     5821 ggcggtttga tggcacgact atcaaaattc aatatcgaag gtgtagatga tccgatttat
     5881 cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta ctggtctaaa
     5941 gtattcccag ctaagactgt actaactgct aacggtgaat acgtacaagt attaccagta
     6001 ccagtttcag acttgcagtc aactgctgtg ccagaaggaa aagcagttat tggggtagct
     6061 tcagattact ttatgggtgt aggatctaca ctaaaaattg aagcttcaga tgaataccat
     6121 tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca acctaaacgt
     6181 aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac aactacaaaa
     6241 ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta tattctttgt
     6301 cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa tctgaagaaa
     6361 ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat tcctattttt
     6421 tttgagaagg aatatggcgt tgaagttcat gtgtacgatg atctgcggga cgacaaagag
     6481 tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga tcattcgcgt
     6541 gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag aattaatttg
     6601 cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag ttatctaagt
     6661 cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat ggcaaatatt
     6721 gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg cgcacggtgg
     6781 attattaacc ttcctaaagc gaattattgg aaaaaggttt atctagattc taatcggcta
     6841 tatcgctacc ttagagggca aaaaacaaat gtacagattt ggacagccga gatgtgggca
     6901 cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga attaaacttt
     6961 tgttttgcta ctgatccaat agaaaaattt aaagaagtaa aaatcttgca taacgctgga
     7021 gtaacaacaa atgatgaaga tttatttttc aaagggagat acgtgacttc cacgcctttt
     7081 gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgc taaagcaatt
     7141 aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata gctgtgaagg
     7201 ataatattta cgttacctgg aacgaagaag atgaatcaat taaaaagatg atagctaaaa
     7261 atgctgttta tttacaaagt aaagtgagta caactctttc tttttctcct gatagcttag
     7321 aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta ggtgagtttg
     7381 aacaaaattt cgctagcgag ttattaggtt tcattcaaca ttatgcgcta caagaatata
     7441 ttgcaggtga tgggaatggc gaataatcgt agactcgaag aaacgttcaa cgatggttgg
     7501 ttaaagattt taacgcaaac caccaaaaga aatgaactag gaaaaaagat tggtgtagaa
     7561 gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag tgatataaca
     7621 gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt tcatccaatc
     7681 gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt
     7741 atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt gggtgaatat
     7801 ggtgaatgat aattctaaag aaaaagaacg tttaaataag caaatttctg ctatcaaaac
     7861 ttccttagaa gaacatttta agctcaaact ctttcaagac tctgttggcg aggatgagct
     7921 acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga tcactgaacc
     7981 aaaatatagt gtgggtcaaa atctatatct aactttctat tcagaaaata gagaagattt
     8041 aacaggagat tcactagata ttatttcatt gattcaaaat cgttcgattc gttttcagag
     8101 aatggatccc aaccatttaa aactagaaaa tcaagatcgc tatatcgatc aattggtatt
     8161 tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg gagctaaaaa
     8221 taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac
     8281 tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg attacagaaa
     8341 aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa aatagtcgtc
     8401 cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga aaatttgagt
     8461 atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg gacgaattta
     8521 tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt cgtcaattcg
     8581 ataaattaaa taaatagggg gaacaacaat ggctaaaaca aaaactgtag taacaacgtt
     8641 cgataacgta agtatcaaac gaattgcttt taattttaag aacgcaggaa atgcaatcgc
     8701 aacgaattgt aatggacaat tagatggcga aacagaaatg caaacggtgg ttaaaaaatg
     8761 tggagcgaca gaagtaaaat caaaatctaa accaatcaat atgacggtaa caattactgc
     8821 acatgtaccg atggaagttt atcgacgttt caatgggttg aaacaagatg gacgtattaa
     8881 accaggcatt tactcttacg gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga
     8941 gatcgtggat gacttcgaag aaaatagaaa gttagttggt atgttagcat gcacttcgaa
     9001 tacaggatta acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga
     9061 aacaaaagtt atgcaagatg aatttggtaa attttatcat gaagcaattg ttgcagaact
     9121 tgaagaagac ttaacagatc aatggatgac gaatctatct gctgatgtga ttaaaaaaac
     9181 ttcagttgtg acaactacgg ccactcaatc acagtaaaaa aaacggaggt agcgaaatga
     9241 acgaagatta ctcaaaaatt gaactaaacg atggaacaat tttgaattta gaacctaaac
     9301 tgaatatcaa gaaattattg atgatcaata gagattttaa cacagacgag tttgcaaaaa
     9361 tgactgtggg aaaaggatcc atggatattt ctgttattca aggtgcaaag gctgtgtata
     9421 ttgcttaccg ccaagcgaac atgactgatt atatttcatt cgatgaattt atcgataaat
     9481 gggattttga tatggctact gccagctata tttatcaatt gatgatgttc aaacaagcac
     9541 gcgatgctta tcaaaaagaa tttgaaaaag caaataagga aaaaaagctt caaaagtaaa
     9601 aattccaaag ctcttagttg aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt
     9661 ttctatgcct tcagatttgg ttttaagcga tatctgttta gatgacattt tgcaaatggc
     9721 ttacaacaag agtgcttatg aaggatggaa aaactatgca ataaaccaat cccagaaaaa
     9781 ctaaagaaag gaggtaaaaa atggctaaaa agagaacaga agcagaagta actttcatag
     9841 ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta actaaaaata
     9901 gagcagaatt aaaactagaa caagctcaat tacaacagac tggttctgaa tcagacaagt
     9961 taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagtcaa aaagttgaag
    10021 taactagcca acgtttagcc aatgccaaaa aatattatgg agaaaattcc accgaagttc
    10081 agaaacttga gagagaactg attaaccaac aaacagcgca acaacgtttg tcaaacgaaa
    10141 ttgataaaac gagtaatgca ctagctcaag caaaaggcga aatacagacg tacgagtcta
    10201 caatgcaaca gttggatagt gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat
    10261 ccgaatacaa aaaatggcaa gcaactgctg gtcaatcagc ttctgaatcc gagaaattag
    10321 cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc agagaaaacg atagatatcc
    10381 tgagacgaca gttagaagct acacagtctg agtttggcgc tacatccaca gaagcaatgc
    10441 agatggaggc gaagcttaat gatgctgaac gtgaatttga agagttagga caagctgcta
    10501 aaaatgtaga tacaactaac ttggacgata tcggaagcaa aatagatatg aataatctaa
    10561 tggaagcttc tgacgtttta agcgacattg gcgataagct tacagaatta gggaaacaag
    10621 cagtggactc tgctaatagt gtaggtagtt cccagagtaa aatacaagct aattttggtt
    10681 tgactaaaca agaggctgaa gaattaacga atgtagccag agacatttat tataaaggtt
    10741 ttggagaatc gttagatcag tccacagatg cattgatttt ggtaaagcgt aatttaggcg
    10801 atttaaataa tcaagattta caaaatatca cggaacaagc tatggtccta gaaaacacca
    10861 tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc aatttcggct
    10921 tgagcgctca agatgcaatg gatttaatgg tttcgggtac tcaaaacggt ttagataaaa
    10981 cgcacgaatt aggcgacaat atggcagaat atagccaatt atggagtcaa atgggatatt
    11041 cagctgatga aacgttcgga atgcttcaaa atggtttaga tgcgggtgct tataaccttg
    11101 ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt aacagatggt cgatttgagc
    11161 aaaacatgga tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg aaaaatggcg
    11221 gaggaacaca aaaagacgtt attaattcca tgattcaaga ttttagcaat atggatggtc
    11281 aatacgacca attaaataaa gcttctacaa tttggtctgc acttggcgaa gataacgcga
    11341 tgaaagttgt ccaatctttg actgatgtta accatacatt tgatgatgtt agtggatctg
    11401 cacaaaaaat gaatgaagat tctactactc cgttacaaga attaaacgga aaaatagctg
    11461 aattaaagga ttcattagct cctataggca acacaatcat agatgcactc gaaccagtaa
    11521 ttgattttct aggaaagatg gctgatgcgt ttaataatct tccacaacca gtacaggatt
    11581 atgccgtagc gattggcgga ttgactgctg catttacttt attaatgcca ataatagttg
    11641 gcttcatggc tctaggtggt cctactacat taataatagg agcagttatt actgttattg
    11701 ctggagttat agcaattata aaaaactggg gcgcaattac tgactggttt aagggaatat
    11761 ggagtaaatt cactgattgg ttgggtggta cttgggaaag tataaaagaa ggtgcctcat
    11821 cagtttggga tggagttaaa gaaacctggt ctggatttgt agattgggtt caagatattt
    11881 ggcaaggagt ttctgattgg tttggagagc tatggagcgg attagttgaa ggagcttcca
    11941 acatctggca aggagtccaa gagacttggc aagcattcgt tgattgggtt tcaaatattt
    12001 ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga attgttcaaa
    12061 taccatggac cttaataacg tcattgattc aagccggtat taatattatc gtgggtattt
    12121 ttgatgtagc tggacagtta ttaggcgcag cttggcaagc tgtttggaca cctatttctg
    12181 atttccttaa aaacacttgg gatactatga cacaatgggt aagcatcgct tggaatggaa
    12241 ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat ggtatatgga
    12301 cagctattag tactacggct tcaaatattt ggaattcaat tagtgcaaca gcttctagta
    12361 tttggaacag tatcaagaat acaatcacta gcttggtaca agcagctgct acagtaattc
    12421 aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca atcagctcta
    12481 cagcatcaaa tatctggaat agcgtgacta gtagtataag caatgctata aacgcagcta
    12541 aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gatcagcgga atttggaacg
    12601 gtatcaaaaa cactgctttg aatctttgga atggaattac aagcactatt agctctaaag
    12661 taaacgatgg aaaaaatgca atttcaagcg gttggtccaa tctaacaggt attgtttccg
    12721 acatattcaa taatgttaaa agtacaattg ctaacatttg ggaaggtatc aaaaagactg
    12781 ttagcgctcc aattgattgg atcagagata aaatcagtag tatctttgat aatttgaata
    12841 tttctatacc acatattccg ttaccacatt ttaaattgag cggagaattc aatccattga
    12901 aggggaaaat cccaacgttg ggtgttgatt ggtatgcgaa aggtagtgtg tttaattctc
    12961 cgaatattat cggtgtcggc gaagcaggac ctgaagcagt tttacctttg aaaagatctg
    13021 tgctgcaaga aattggtgat cgtatcttga gtagcacatc agtttcatct agggcacaaa
    13081 cgattcaacc tgtgaataac tacgaattca atttcacaat tgatggtaac gcagatgagg
    13141 ttactatgaa gcaaacaact caacaaatca ttgatagcat tacaaaagtt caaaatgata
    13201 atgcttcggc atggcgttaa acaggagagt atttctcctg tttttttagt attaaaaagg
    13261 atgtgaaaaa atgactgatt gtatacattc tataatcgat ggatttcctg attatttgca
    13321 taaattggct ttagctgaaa gaccaaccat accttctcca aaaagacaga gagttgaaac
    13381 ttctgtttta ggaaggttag gtggcttagt acaagattac tcgtttgaag acatgtcgtt
    13441 tacattgcac tataactatt tagaggatgt ggaagaccat caagcgttca agcaatcgtt
    13501 ttatatcatg cgtcattggt taaattatgc aaagaaatta gaattctctg atgatcccaa
    13561 cgtctattat gttatccaga ctatcgatat tggggatgca gaaaacgata ttgttgaatg
    13621 gggagagttc gatgtaaata ttactgcgaa accattcgca agagttcaag aagatgtacc
    13681 tataaccgta gataaaccac agtcatttaa cttgctgaat aatagtttag aagaaagttt
    13741 tccaaagatt atcatcactc cttcagctac ttcatgccag ttcatcttaa atgattatgt
    13801 gtttagtttt gaaggcttag tagtaggaac tgacgtagtc attgatagtg atttgatgct
    13861 ttgctacgaa gagcaatcgg acggagatat tttagatcgg tccaacaaaa tgaagaccat
    13921 gcaatatccg acattgcaag tggatattaa ttattttaat tgtactggtt tgagcaaaat
    13981 acaaatttat cgtaatgggt taaggtaggt gaaatagatg atcgataatt taataactat
    14041 ttacgataaa aacgacgcga ataatttagc tgaacattta tatgatacgc aaggtttagg
    14101 cgctttgtca gactggttaa cagctactgt tagcaataaa ttaaacggag ccgagatatt
    14161 tcagggtact tatccaataa gcggaactaa tgcagatttg attgtagaag gacgtattat
    14221 tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta cggatttatt atgcaaagac
    14281 ttctgtaata ggcaatacga tagaagtaaa agctgaacct attttcaatg atataagaaa
    14341 atcggtgttg aataaatatg acagtggaac agaaaagatc actgctagtc aggcatggca
    14401 aaacgcaaaa actttagcga aaccagttat tccttcacag ttttctttct cgtcattagt
    14461 agatacgctt gctaatgtga agatagaaaa ggcgaatttt ttagaattct ttggtggaaa
    14521 agagggatct attctagatc gatttcatgg ggaatttcta aaagataata acacattacg
    14581 tcatgaaaaa aggctaggca cggatcataa aatcaaagcg atttatacta aaaacttaac
    14641 tggtcttgac ttagagattg atgctcaaag tgttttagtt ggagtttatc cattcattag
    14701 cagctcttca gaaggagaag acgagatcac tctaccagaa gaagttattt tcacggatta
    14761 cgtggatgat tatcctgctg gatatgtttc ttttgttgat tttaaagaca aagcgactga
    14821 tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa
    14881 accacaagtg agtggttcga ttgaattagt accattgagg catcaaagag gctatgaaaa
    14941 atttgttgat ctagaaaaag tttcgatggg tgacggagta gatgtgtatc atccacagtt
    15001 aaaagtgaat atgtcagcga gaattgtgga atatacgttt aatgttctaa ccaattcata
    15061 cgataaatta gttgtaggaa acgtcaaaac aaacttctta gaaaacacag agaataatgt
    15121 cagcaatttg attaatgatg ccattgatca attgaaaaac ggtggcgaaa tcagtgattt
    15181 actcaatgat attgtagatc atcaaactga tatgattact ggtcaaaatg gtggttatgt
    15241 tttattagat cctaaagaag cgcctagtcg tattttgatt atggacacac cagataagaa
    15301 taccgcaagg aatgttttac aaatcaacaa tgctggtatt ggtttttcta aaactggcat
    15361 taatggaaca tatgaaacgg catggacgtt agatggcgga ttcaatgcct cgtttattac
    15421 ggctggtgaa atagtaggaa ttactattag aggtactaca ttaattagtg atggtgctga
    15481 ttatagaaca agtattgcta atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga
    15541 tattatggag ctagaagcac gtgattatgt aagtgctgat gccggtattg tatcatacac
    15601 catgaaaact ggtggtggtt tcatgattag aaatccacag ggtaacttgg tttttagtac
    15661 gtgggataat ggtaataaca gaccgtttct atcttttggt gcgcccaatt tcaggtatag
    15721 caatgctagt tatgtaactt ctggcgacgg tagttcttta agcattaatg gtagtgcggg
    15781 taactcatgg gaatttaagg tagctggtag gactatgaaa tttactagtg atggtatgct
    15841 aacgttacca ggttgttttt ttggttcatg ggaagatggg aaacttgcta ggtttgaaca
    15901 atcaacggta caagtatata aagattttac tgttagaggt actaaaaact caactgtacc
    15961 aacagaacat tatggacaac gactattgaa cgcttatgaa actccagaat attatttcgc
    16021 tgattatggg gaagccgtta caggtgacga tggtaaagtt cgtgttgata ttgaccccat
    16081 gtttgctgaa acagtaaatc taagtcggta tatgacacat gtgacaccta cagaactagt
    16141 tttgtgtgct gttactcatg aagatattga ccatttcatc attgaaacta gtaagccaaa
    16201 cgtattagtt agatggaatt tagtggcaca ccgtctaggg tatgaagata ttagattaaa
    16261 agaggataca gcatatgata gcacagtgct tgaccaaaaa cgtttttaaa acgaagacaa
    16321 ggaggtatat aaatggctag cagtttatat aatttggctt tagatttcag caaagaatta
    16381 aactacacca aagctattat ggctcgtcaa ggtgataaag ggattacggt gacggttaaa
    16441 ccgtttctaa atggcttgca gatggatacg agtggcggaa catttacttt aaaaggaaca
    16501 acaccatcta accgttacgt agataatgtt gcaactagtg taactagtga agaagtcacg
    16561 ttttctcttg atggcacatt tatgagtgaa gcaggatatt ataaacactg ctacgtagaa
    16621 tatagaaaag acaatcaaat tttaacaacg caagatatca tttttttctc actaggagtg
    16681 tctgacattt cgcaaggcca agccgatgaa tatgtttcgc aattagaaga gttgattcga
    16741 aagtacaacg aaacttttga tgcttttatg gctgaaatca aaggtagagt ggatagctta
    16801 aatcaacaga ttactgattt aactggtcaa gctaaaacgc tacaagacaa gttagatgct
    16861 ctgaaagaag aaatttctaa gttaggtaac ttacaagtga tgtacagtaa cagcatcgac
    16921 ttcgggggat atgattatag cggtaagccg aatttaatgt ccaaactaaa atcgagcgat
    16981 tttaacgttg gttaccacgg gtcactaact ttggataacg aaaagttaca ttttacttct
    17041 gatggtacag gaagcattat tatgtttacg cgtattaata cacctcagct tactagtggg
    17101 aaaacctata ctctgagtgc gaaagttcga tttgatgaag gaactacagg agctattgat
    17161 aaattacgtt tggtgtatcg tacatcacca ggagaaaaga tattattgga agcaaatagt
    17221 acaaatatta caacagatga tgtagggaaa gaaataacaa tcaaaggtac agctaacgtt
    17281 aattatcaaa tcacaaattt agatcgattt tatatgagta ttagctttgt tgacagggat
    17341 aaaataaatg gcggatttaa gttgtacgac atcaaaatcg aagaaggctc aacagccaca
    17401 ccgtaccagc ctaatctact tgatgcaccg tattatttga gtaaggtggc tttgggtgag
    17461 aatctaatta aaccagaatc acaacaacca gttactaata gtaactatct tattaacacc
    17521 tataacatta aaccaatggt aaaaggtaag aagtatacca tcacacttga aggaactaag
    17581 ccagcaacac aggtttttag accatttttc acacgagcaa caggagatgc atgggaggtt
    17641 ggtgacttac aaccagtaga aggcttaact aatgtgtggt ctaaaacatt tacagcagca
    17701 gatgactcac accctactac cccacaagtg cagatttatc aagtaccaag cacaagtgta
    17761 ggacaatgta caattaagtg gttaaaacta gaggaaggca atactcgaac cccgaatatt
    17821 agtgagtata aatatcgtgg tactggtatg cgtgattcaa acaatccaaa agattatgtt
    17881 tgggatctag caccagaata tgtcgaagat aatttggcca cagatattaa aatttctgaa
    17941 attactggta aagcaaacaa ttataccgat gggaaagtat cggagattaa ttcgcagttg
    18001 actgcttcaa ttaatgaagt agacaccaca gctaaggatg ctcaaacaaa agcgaatgct
    18061 aatgcgactg ctatagatga attagacaat aagatcgatg aacgcattaa tgatacagct
    18121 actaccacat taacagttac aaacgggaat accggatcag caaagcttta tcgtgaagga
    18181 aaaacagttt ctatatattt tgtggcttta aacggaaaac gcagtggtgg aaatgattca
    18241 acgatactaa caattccaga aggctatcgg ccaccaatta gttttgagca actggttggc
    18301 tcgatagacc gttctacttt gaacagtgct cagttatcta ttggtgcaga tggagccatt
    18361 aaatggcgaa gaaactcaag ttatggatcg gattatacct ttgcaattac ttacacgatt
    18421 tagaaaagcg tgaatcgata tgaaggcagc atatagacca attgaacctt acggattcga
    18481 gcaaatcatt gtgaatgatg aagaacattt accggaagaa tgcacagaag tcgaaccacc
    18541 gattccaaat tggaaaccga aattcaatta ctgggaggga aataaatgaa aaacatttgg
    18601 aaatatggac gtactggcgg agagtacgca ggaaaagtat tggacgacat gcttgtatcc
    18661 gttccttaca cagatcagcc accgcttgaa ggaattcgtg ctgatggcga accgttaaca
    18721 atcgctgatc aaatgtttga tcctaaattg aatcaatgga ttattttagc gaacgcacta
    18781 gatcacaacg atttaaacaa tctcaaagca atgtatgagt cgttagaaaa tgagaacggc
    18841 gatttaaaac agatcaatgc caaactcatg ctaagcgatg tagcaattaa acaggaaaat
    18901 actgcattga aagaaaaagc ggatagttta gcacaaatca attcaaaaat gatgcttgct
    18961 tcgttacaaa atagcaaaga catttcagaa attaaagagc aactaaatcc agcttcaaag
    19021 ggaggtgagt agtatgttta gttttagcga tgtgaaaatg atgtatgatt ggggctgttt
    19081 cactaacgaa caagtaatgg ttttcgttcc gttgtgcatt actgaagaaa aagcagataa
    19141 aatcattagc aaagaagaga gcgcatctta attgatgtgc tttttatttt gattcaagga
    19201 gttgtcacat gattaattta ggggaatggg gagcgatagc aggatcaata accgctatcg
    19261 tttctttgat tttattagta ataaaaccaa ttactgcatc tttctcgaag attactgaga
    19321 ctctttcaaa agtaagtcac aatttagatt tgctgactaa agatttagaa tcgagcaaat
    19381 cagatcgatt gatgattcat gaagaactaa agaaacacga tgaaagatta gatacacatg
    19441 cagaaaaatt ggtagaacac acgcaacaaa ttaaaacttt
//
LOCUS       DAGCXG010000040        19450 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.041, whole genome shotgun sequence.
ACCESSION   DAGCXG010000040 DAGCXG010000000
VERSION     DAGCXG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.041"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            126..3653
                     /locus_tag="MB022_001495"
     CDS             126..3653
                     /locus_tag="MB022_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBT4466498.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="MB022_001496"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="MB022_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBT4466499.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="MB022_001497"
     CDS             7441..7782
                     /locus_tag="MB022_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466500.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="MB022_001498"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="MB022_001498"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBT4466501.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="MB022_001499"
     CDS             9139..11559
                     /locus_tag="MB022_001499"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBT4466502.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="MB022_001500"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="MB022_001500"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBT4466503.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="MB022_001501"
     CDS             12993..13730
                     /locus_tag="MB022_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4466504.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="MB022_001502"
     CDS             13744..14559
                     /locus_tag="MB022_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4466505.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="MB022_001503"
     CDS             14572..15219
                     /locus_tag="MB022_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4466506.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="MB022_001504"
     CDS             15235..15891
                     /locus_tag="MB022_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4466507.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="MB022_001505"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="MB022_001505"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBT4466508.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="MB022_001506"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="MB022_001506"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBT4466509.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="MB022_001507"
     CDS             18233..18751
                     /locus_tag="MB022_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4466510.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="MB022_001508"
     CDS             18858..19358
                     /locus_tag="MB022_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4466511.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3340 c         4177 g         5392 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc
//
LOCUS       DAGCXG010000041        19246 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGCXG010000041 DAGCXG010000000
VERSION     DAGCXG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.042"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..909)
                     /locus_tag="MB022_001509"
     CDS             complement(<1..909)
                     /locus_tag="MB022_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4466512.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(906..1370)
                     /locus_tag="MB022_001510"
     CDS             complement(906..1370)
                     /locus_tag="MB022_001510"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBT4466513.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(1375..2073)
                     /locus_tag="MB022_001511"
     CDS             complement(1375..2073)
                     /locus_tag="MB022_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4466514.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(2060..2446)
                     /locus_tag="MB022_001512"
     CDS             complement(2060..2446)
                     /locus_tag="MB022_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466515.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(2593..4263)
                     /locus_tag="MB022_001513"
     CDS             complement(2593..4263)
                     /locus_tag="MB022_001513"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBT4466516.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            4446..4679
                     /locus_tag="MB022_001514"
     CDS             4446..4679
                     /locus_tag="MB022_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBT4466517.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(4778..5209)
                     /locus_tag="MB022_001515"
     CDS             complement(4778..5209)
                     /locus_tag="MB022_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBT4466518.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            5288..5701
                     /locus_tag="MB022_001516"
     CDS             5288..5701
                     /locus_tag="MB022_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBT4466519.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(5739..6886)
                     /locus_tag="MB022_001517"
                     /pseudo
     CDS             complement(5739..6886)
                     /locus_tag="MB022_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
     gene            complement(7008..7604)
                     /locus_tag="MB022_001518"
     CDS             complement(7008..7604)
                     /locus_tag="MB022_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4466520.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(7804..10446)
                     /gene="alaS"
                     /locus_tag="MB022_001519"
     CDS             complement(7804..10446)
                     /gene="alaS"
                     /locus_tag="MB022_001519"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBT4466521.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            10834..11265
                     /locus_tag="MB022_001520"
     CDS             10834..11265
                     /locus_tag="MB022_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466522.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(11310..12659)
                     /locus_tag="MB022_001521"
     CDS             complement(11310..12659)
                     /locus_tag="MB022_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4466523.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(12729..13892)
                     /locus_tag="MB022_001522"
     CDS             complement(12729..13892)
                     /locus_tag="MB022_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4466524.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14021..14344
                     /locus_tag="MB022_001523"
     CDS             14021..14344
                     /locus_tag="MB022_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466525.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(14527..15876)
                     /locus_tag="MB022_001524"
     CDS             complement(14527..15876)
                     /locus_tag="MB022_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4466526.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(15945..16898)
                     /locus_tag="MB022_001525"
     CDS             complement(15945..16898)
                     /locus_tag="MB022_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBT4466527.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(17000..18190)
                     /locus_tag="MB022_001526"
     CDS             complement(17000..18190)
                     /locus_tag="MB022_001526"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBT4466528.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(18835..>19246)
                     /locus_tag="MB022_001527"
     CDS             complement(18835..>19246)
                     /locus_tag="MB022_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HBT4466529.1"
                     /translation="FSIIENMSFSKNLSEAAELSLEGKIVQDADRIEALGAIGILRTA
                     YFGGGHGHPIFDPELYPQTFKDKENYRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKR
                     RETFMREFLEEFFLEWSIDDENFTPDNWIKNNKK"
BASE COUNT         5595 a         4063 c         3319 g         6269 t
ORIGIN      
        1 aaatgcaatg ttttttctta atttttctaa agttttttca aaaacttctg gaagaggtgc
       61 ttcaaagatc atcttttcgt ttgtagtcgg gtgcgtaaaa cctaacattt gcgcatggag
      121 aaactgacca ttgcctttca aagttttctt cggtccgtat actggatctc cagctaacgg
      181 ataaccgata tacttcatat gcacacggat ctggtgtgtt cttccggttt ctagttttaa
      241 ttcaaccaga gtaaatcctt cgaaacgttc caatacagta aaatgtgtga cagccgactt
      301 cccatcttct cttatcgcct gcattttccg gttttccttc gaacggccga ttggcgcatt
      361 gatggttcct ttttcgtgag gaatctctcc atggactaaa gcaacatatt tgcgcaaaga
      421 ggtcttatct tttaattgct gagctaatga ttcgtgtgcc tggtcatttt tggcaaccat
      481 tagcagtccg gaagtatctt tgtcgattcg gtgaacaata cctggacgaa taacgccgtt
      541 gatcgtagaa agatccttga tatggtacat cagcgcattg accattgtgc catgcgtatg
      601 acctgctgaa ggatgaacga ccattccttg aggtttgttt atcaccgcta catcttcatc
      661 ttcgtaaacg atcgtcaacg ggatattttc tgggaccaca tctaatgttt ctggttcggg
      721 gatcaagaca gtaatctcgt ctccttgttt tactttgtaa tttgctttga ctggctgtcc
      781 attgacagaa accgctccat ttttcagcca cagctgaatt tgcgaacgac tgtaatcggt
      841 gaataaatta gtcaatactt tatcgatcct accagattca tggtgaatcg tcgttttaag
      901 ttctgtcatt tttttctttg atccttttca tctaaaataa tataaattaa catacaaatc
      961 actccacata caagtgtcac atctgctacg ttaaaaatcg gaaagctcat aaaatctgtc
     1021 tggaacatat ctactacata gccaagacga atacggtcga taaaattgcc aatcgcacca
     1081 gctagaatca aactcaatcc aatcatgaac cagcgattac ctttttcacg atttttaatc
     1141 atcaatgtga gaatcacaac gactgcaatg agtgtcacta tagtaaagaa ccacatcttc
     1201 ccttctagca tactccatgc ggctccagtg tttcgaatat gattcagtga caaaatacct
     1261 ggtataagat ttttcgtctc tccaagcgct agattatcag tgatccacca tttgaccaac
     1321 tgatccactc ctactagcaa agcgctgaat atccaataaa taattaacaa aaaatcaatc
     1381 cttttctatt tgtttataaa tatcacgagg tcgtacgatc ccgagcagat taccatcggg
     1441 attcccatct ttagtgataa gtacggcttc caaagtccct tgctcacgaa acatcgcttc
     1501 aacctcgtaa atatgtgttt tagctggaac aaaatgataa tttgtgtact ttccgttttg
     1561 gatgatcaaa tccaacgctc gtttatttgt taaatcaacc ggaccttttt ggctttcttt
     1621 tgctagccaa aacccgatat tacgtaaagt aatcaatgct acaaattttc ctttattgta
     1681 taacggaaat tgtgaatacc ttttttctgc tactatcttt aacagttcaa gtaacggaag
     1741 atcttgttca aaacctgtta cacgttttgc aaatcgaggc agcacggttt caggctgaat
     1801 cagttcttgt tcgatcagct ggattcgctt gactgtccaa tcatttggct ctgcaatgac
     1861 aaaatcgact gcgatccgat catggacgat cgcattcctt aactgagcaa gctgtaaaag
     1921 atcatcttcg tattttctga tcattaattg ttttctttga gctaggcgtc tgacaagttc
     1981 ggtaaatccc atatttcgtg catttcccat ctcgtctctc atccattttt cgattcgatt
     2041 gaaactgatt aaaaaaagat cagagtttgc cataattttc ctccttgatc aaatatgccc
     2101 ggttttttat atagaaaaag aacgcaatca acacaataac tgcaaaaaaa ctgaaaaata
     2161 aattttgtag gaaaacggcc cccaacccag ttgctagaaa taaaaacgga tagatttgaa
     2221 gaatcaagta attcattgtg atcacataag caggctttgc ttctttcact gtataaacgg
     2281 ggctttttag ccaataaaag gtgacctgca tcgtaaaggg cacttccaga tcaagcgtta
     2341 tcttttgttc atgactgaga acactgcgtt tttcattgtt gattcttagc tgaatcgagc
     2401 tacccatacc gtaaaaaccg gtttttctta taaatgtcgt tttcatcata ccctcctatt
     2461 atgctttagt atagctcaaa atacaataaa gaaaaagaat gatcctagtg taaacaaaat
     2521 taattcccta tcgtctaaaa aaagaagctg tagtcaaaat acagctcctt cgatggtaga
     2581 ttataatcaa gcttaaaaca atcccaacac ttctccgtca tttgtcacgt ccatattcaa
     2641 tgcagcaggt tttttaggca aaccaggcat cgtcataaca tctccagtca aggctactaa
     2701 aaatcctgcc cctaattttg ggatgatttc gcggatcgtg atggtgaaat cttccggtgc
     2761 acctagcagt gaaggatcat cagaaaatga atactgtgtt tttgccatac aaatcggcag
     2821 tttgtcccat ccattcttcg taaactcttc tatttgtttt tgcgcttttt tgctgaaaac
     2881 aacaccattt cctccgtaaa ttttcttgac gattgtttct gtcttttctt gtatcgtttc
     2941 ttctaggcgg tataatggct tgtaattagc ttcttttcta tcaatcaatc gtactacagc
     3001 ttttgctaaa tcaacgccgc cttctgctcc atctgcccat acactcgctc tcttggcaag
     3061 aatcccctga ttttcacaca agtgctccaa cagtgtgagt tcattgtctg tatctgttac
     3121 gaattcattg attgcgacga tcacaggtag ctgatattgt tccatattac gtatatggcg
     3181 ctttaaattt gcaaaaccta ctttcaaagc atcaagattc tcatttttaa gttcatcttt
     3241 tttcattccg ccatgcattt tcaatgcacg gacagtggcg acaatcacca cagcatccgg
     3301 cgcttttttc aagttaggta ctttgatatc taaaaatttc tctccgccaa gatctgcacc
     3361 aaatcctgcc tctgtgacaa cgtaatctgc taatctcaat gctgtcttgg tagctaaaat
     3421 actattgcag ccatgagcga tattggcaaa cggtcccccg tggacaaaag ctggtgttcc
     3481 gtaaattgtt tgtacaagat tcggcttgat tgcatctttc agcagtaatg ccagagcgcc
     3541 ttctgctttc aagtctccag cagtcacagg ctgacggtca aatgtatagc cgacaacgat
     3601 ccttgcaaga cgagccttta aatcttcaag gtctgccgca agacacaaga tagccatgat
     3661 ttcgctggca actgtgatat caaacccatc ttctcttgga acaccttgga ttggcccacc
     3721 caagccaaca attactttac gcagttcacg atcattgaga tcgactaccc gtttccaaat
     3781 cactcggcgg gcatcgatgt tcaattcatt tccttgatga atatgattat ctaaaagagc
     3841 agataatgca ttattagctg ttgtgatagc atgcatgtca ccagtaaaat gcaagttgat
     3901 atcttccata ggcaatacct gtgcatatcc tccgcctgca gcaccacctt tgattcccat
     3961 cacaggacca agtgagggtt ctcgcaatgc aataacagat tttttattga ttttattgag
     4021 cgcgtctcct aaccctacag tgatcgttga ctttccttcg ccagctggtg ttggattgat
     4081 ggaagtaact aagatcaaat gcccctcagc gttatccttt gatcgattga ttccagaaaa
     4141 attgattttc gccttatatt tcccatacag ttccaaatcg tcttccttaa gatctaattt
     4201 actagccact tcaacaatcg gttctagctc tgtttcttgt gaaatttgca gatcattctt
     4261 catttgatta ccccttttca cgaactttat tcaacaaaat atcttctttg ttaagatatg
     4321 ccaattatac ctttttttat tcagaaaaaa aacataaaat tacaggtttt taatttattt
     4381 gacgtactta taaatactct ttataatgaa ggctatgatc agactttaga aaggtcgtga
     4441 gtgctttgac cacaggaatt gtaaaatggt ttgacaacaa aaaaggatac ggctttatca
     4501 gttatgatga cacagaagaa atttttgtac attttactgc gattgaagaa gagggattca
     4561 aaactttgga agaaaatcaa gtggtcgaat ttgagatcat cgaagggaat cgaggaacac
     4621 aagctgccca tgtcaagaaa ggcaacgttc agactaccga tactagtatg catgaataga
     4681 taaacaaata gagtgagacg ctaaacggga tgaaccgtac gtctcactct tttcttttcc
     4741 tgtaaaaatt cgttttttta ggttctgatt tttaaaatta gctttttctt tttttcgtat
     4801 aaggataagg aattgattct gatttgggtc cgtttttttc aaaatgatca aaaaggacta
     4861 cttgatcttt ttttcgcatc atgaaaacag atggggcttt ctcatcttgc aacatcattt
     4921 gcaccccagt attagttggc gtcaaagatc gaatctgtcc gacagatatt tctcggtctt
     4981 tccaaaaagc agcataacgt ataatcacag agtttgattt taaaataaaa gaacgccgca
     5041 aacctagaaa tagaaaaaca agaaaaatac ctaaaacaaa attgcttttc caataaggac
     5101 ctgtgttttc taaagaaaga atcagactaa aaaacagaat gatcaaggtc aatgaccaat
     5161 agatgatcgt atgtgccagt tcaggctgcc atcgaatatt ttttttcatt ctctaacctc
     5221 tcaccgattc agtaatgata taatcttacc ataacattag tttgaacaaa aaggagaatc
     5281 tctttttatg ttaaaagtat acattgatgc ctctacaaaa ggcaaccctg gtcctagtgg
     5341 aggtggaatc ttgattgtct atcaaggtaa acaagaacag ctatctatcc cgctttctat
     5401 agggacaaac catcaagcag agttcgaagt ctttttaaaa actttagaat ttctaaagaa
     5461 aaacgactta cagaatgaaa cgattttctg tttttcagac agcaaaacac tagtttctac
     5521 ggtcgacaaa aacgcaacga agaacgagct cttccttccc taccttcaaa gaatccaaga
     5581 acttctatcc gaattttctt tattgatcct gcaatggttg cccgaaaaca aaaataaagg
     5641 agcagataat cttgcacgtc aggctctcca taaacaatta aaagaaaaaa agactcttta
     5701 aattaaaaac tttaaggagt ctttttatag tttctcattt aatataaaag ataattgtga
     5761 tcaaacgtaa tttccaattg gtaatctccg attttttctt ctatttgact acgtaagatt
     5821 ctttctagtt ctgtatcaac aaactggaat ttctcaggga ccactacatc aaattgcagc
     5881 gtttttcctt gatgaattac tctaaaatca tgaatattaa gccctgtacc tgtttcaaga
     5941 agaatttgct ttaactgccg caagatatta tagtattttt cgttatcaac aggcattgga
     6001 tccaagtgac agacaagttc cacatccaaa gactttttaa agtctttttc tatcagatca
     6061 ataatcttat gagccacatt caaattcatt gattcatcta cttcgatgtg gactgatgca
     6121 aagcgtttat tgggtccata gtttgcacca ataaatcatg atagccaagt atatcatcgc
     6181 aagcatttaa atgctcttcc atttcttcaa tctctgctgg cgttgggcga cttcctaata
     6241 gttcgtatac aaaatttcga agcatcgtca atccactata aaggatatat aatgctaaag
     6301 caaaacctat atatccatca atacgccatc cagttaccca ttcgattgct gctgaaagca
     6361 atacagccag tgtagtaaag acatcattat aactatctgt tgctgtagct ctcaatgttt
     6421 cagaatcaat ggattgcgca ataacctgat acattttgcc ttgtgcaaat ttcaaaaaga
     6481 tggaaatgat taggacaaaa aagacgatgg gtgttaaaga gacattttct ggatggatga
     6541 ttttttcagc agaagactct aaaaattgaa atcctacata agtaatgatg atcgaaacaa
     6601 ataacccact gatatattcg aagcgttcgt gtccataagg atgttcctga tctgctggct
     6661 ttgcagcaat tttaaatccg atcaaagtca atatggatga agcagtatct gatagactgt
     6721 tcactgcatc agccataatg gagacacttc ctgaaaaaag cccgatcatt agcttagaga
     6781 caaacaagat cagattcgta accaatccta agatacccgc tagtactcct ttttttgtcc
     6841 gctgttgcgg tatttgtttc tttttttctt cttgtttctt agccaagttt tttacccctt
     6901 ctagtttgct tgttgctcag gtaaaaagat ggagaggacg attgttttcc tctcctctct
     6961 tcctttttct atctatcgtg cgtcctatta ctattaaaag atggtcatta ttctggtttc
     7021 aacggtgttg ctgcaaataa cgcttttgct agtaatggtg ttatgattcc cgcacctatt
     7081 gcttctggta tcccattgat tgctgcaacc cctgctaagg ttttaaataa aagagcagag
     7141 tctacaccat aggcatctgc cgttgcacct gtataaaata atcccatgaa tcctaacact
     7201 aagactgtat tgaccaatga acccagtacc cctgcaaagg ctgagctgat cgttattgat
     7261 ttattttttt gataaatcca gtggaaaacc aagcctgcta ctaagccaac aagtactctg
     7321 ggaactaccg agataatcgg attcgtgaac actaatgtat caaatggcgt tgttggcatg
     7381 gcataggcac gaacaattgt agcgattccc caaaacaagc caatcaccat gccatctttt
     7441 gggcctaata ctactgcagc aataattact gtgatatgga taattgttaa actaatgaat
     7501 cctaatggaa taaaccccag ccaaggaacc atcgcttgta cgatgatgat tgctaacaag
     7561 atcgcacgta agactagtcg aaaggtctta tttctactat tcattttttc accccttttt
     7621 atttatacta atcgtaaaaa tttctgtcac tttcgtcaac taaagaccat ccgaatccga
     7681 ccatttgatt agatgcgcca tccaattgaa aaaagtatga gggcaagact tgtttgtctt
     7741 cgcccccata atcatgtttc gttcaatgtt tgaacttttg tttttctgtc aaacgtaaac
     7801 gccctattgt tgcgcaaccc attctttcgc agctttaagt gcatctggaa taccagcagg
     7861 atttttcccg ccagcttgtg ccatgtctgg acgtccacca ccgccaccgc ctactttcgg
     7921 tgcgattgct ttaatcaagt cgccagcttt tagtccagct tcatttgatt ctttcgtcat
     7981 tgccgctagc agactgactt ttccttcttg ttcgtttgct aaaacaagaa tatctgaaat
     8041 atttttttgt ttccattggt cagctaattg acgcagctga ttcatatctt tcacttttac
     8101 ttgtgctgca atgattgttt ttccattgat cgtttctgga tctttgaata catcaccagc
     8161 ttgttgattc gctaacttgc tagctaattg ttcattttcc ttttgtagct ctcttacttg
     8221 ctgttgcaat tgttccgctt ttgaaacaac ttctttcaat tgaggtgatt taacaattgt
     8281 tgctacttgt tttagttgtt tttcttcttc ttcaagtagt tcgtacgctt ctttacttgt
     8341 tacagcctcg attcgtcgca cgcctgcacc aattcctgat tcagaaacga ttttgaagat
     8401 tccgatgttt tctgtatttg ctacatgtgt gcctccgcac agttcgattg accagccgcc
     8461 aatatttact actcgaacgt catgtccgta tttttcacca aacaaagcca ttgcacccat
     8521 gtttttggct gtatcgatat ctgtttcaat tgtttcaact gggatagctt cccagatttt
     8581 ttcgttgacg attctttcca tttcagctaa ttcatctgaa gtgatttgac caaagtgggt
     8641 aaagtcgaat cgtaaatgtc caggagcgac taaagaacca gcttgatttg catgctcgcc
     8701 aagtacttct ttcaatgcct tgtgcagtag atgggttgct gtatggtttt tgatgatccg
     8761 attgcgcatc gattgatcga tcaatagatg ataggtttgt ccttcactca ttggtgcttt
     8821 tacttctacg gtgtgcataa attgaccatt aggcgctttt tgaacatctg taacaactgc
     8881 tacgatttcg cctacttcat tcatgatcca tccttgatcc gctacttgcc cacccatttc
     8941 tgcatagaaa ggtgtttgat caaagatcat ttgagcttca ccttctgcga cttcattcaa
     9001 aagagactca cctttaacga tgaccaataa ttgactatct gattcttgtt ctgtgtatcc
     9061 aatgaattta ctttctactt tgatatctgt caataaagca gattgtaccc ccattgattt
     9121 ttcagtactg cgggcagagc gagcacgttc gcgttgtgca tccatttctt tttcaaaacc
     9181 agcatgatca actttcagtc cttcatcttc tgccacctct tccgttaatt ctaccgggaa
     9241 gccatatgtg tcgtatagtt tgaagatgtc ttttcctgct agagtcgtcc cgttttcgtt
     9301 ttttacttct tggatcactt gatttaagat ttccaaacct tcattgattg tttcattgaa
     9361 acgttcttct tctgtacgga taactttttc aataaattct ttttgttgaa gtacttcagg
     9421 ataataactt accatgattt ccccaacaac agggactaaa cgatataaga atgcttgttc
     9481 aatacctaat tttttcccat gcatgacagc acgacgaagc aaacggcgta agacataccc
     9541 tctcccttca ttcgatggca atgcaccgtc gccaatagca aatgaaaggg cgcgaatatg
     9601 atctgcgatt actttgtaag aaatatctgt tactgggttt tcaccatatt ttacttgttt
     9661 gcttagcttt tctacggcgt ggataatagg cataaacaag tctgtctcaa agttcgttga
     9721 cgcatcttga acgattgaga ccatgcgttc caatcccatc cccgtatcaa tgtttttatg
     9781 aggaagtggt tcaaatgtgt tatcaggctt atggttgaat tctgaaaata ctaagttcca
     9841 gatttctaaa taacgttcat tttctcctcc tggatagttt tcaggatcgt cttctgctaa
     9901 atcgttataa gcttctccac ggtcatagaa aatttccgta tccggaccac atgggcctgc
     9961 accgatatcc cagaagttgt cttctacatc aataatgtga tctagtggca ggccaacttc
    10021 ttcatgccag atgcgttttg cttctgtatc ttttggataa actgttacat aaagtttttc
    10081 tggatcgaat gccatccatt ttggatcagt caaaaattcc catgcccagt ggatggcttc
    10141 atttttgaaa tagtcaccaa tcgaaaaatt acccagcatc tcaaacatcg tatggtgccg
    10201 tgctgtttta ccgacatttt cgatatcatt tgttcgaata gatttttggg catttgtgat
    10261 tcttggattt tctggcacaa ctgaaccatc aaaatatttt ttcaatgttg caacaccaga
    10321 gttgatccac aataaggttg gatcgttcac ggggatcaaa gacgcactag gttcaatcgt
    10381 atgtccttta gattggaaga aatcaagaaa catttgtcga acttgtgcac tacttaattg
    10441 tttcatgtca ttacctgctt tctataaatt aaatacaaaa aaacaccatc acagccaagg
    10501 acgaaaactc gcggtaccac cttgattgca atgatgtgta atcattacct cttaaactcg
    10561 ataacgctga gctgcgtcgg tatttctacc aaaatagaaa aagggagcaa tttgattagt
    10621 gactattctt ttttcagcca agaaagaatt ttctgtgagt cttaaaaaac aaatcatatc
    10681 cttttcgtac ttatttagta tacgcattct attgatcact gtcaatatct caatttttat
    10741 tttcatttcc ttttggtcat actccacgat ttttggtacg ataagaataa aggaatcaat
    10801 acatctctgc tattcatgac aaggaggaat atgatgaaaa aaatagttgg agcggatgaa
    10861 tggatacgcg cagctagctg ctttttgaga tatcaagttt ttgtattaga acaagggatt
    10921 cctgcagaac tagaattcga cggaaaagat atcccgcaac aaacctatta tctatttctt
    10981 gatcaaaaaa gaccagtagc tactttgcgt tatgaaaaga aaacacctac ttgcttcaag
    11041 ccggaacgat tttgcgtagc aaaagaatac cgaggaaaag ggttaggcgg tcagttgctt
    11101 gaagctgctg aaaaacgggc ggtttcagaa ggttgtcaga ctagttatgt agtagctgaa
    11161 atgacggctg tagattttta tgaaaaacaa gggtatcata cttgcacaga accttttatt
    11221 gaagatgaag tactttgtat cggaatgaaa aaaagtctag cttaaaaaca agagattgtg
    11281 acacgccttt tgtcacaacc tcttgttttt tatttatgtg aatttttctt atttttacga
    11341 gcagtacggc tttgctgtct gcgttcgatt ttgcgttctt ttttattttt ttctgcaatt
    11401 gcccaatcga tttttttctt ataaccaggc ttgatctttt tctttttctt cttcacaaga
    11461 ccaatcagcg tgggatctaa tacatctttt gatttttctc gtttggcacg tctattacga
    11521 tcgtatgttt tgacgatttc cccgtttttg atttcttttg gttcaaatga gacgcccaat
    11581 ttttctatcg catcaatggc ttgctcatca tttggcgaat ataacgtaat ggccgttcct
    11641 tttaagccat tacgtccagt acgtccgaca cgatggataa agaagtctag atcttctgga
    11701 atttctgcat taataacatg agagacacct tcgatatcga tccctctagc agctaaatca
    11761 gtcgctacaa catattgata ctccagattc tgtacttgtc gcatcactcg ttttctctct
    11821 cttggggtga tgtctccatg aatctttgct actttcaagc cttggttttt caagtaatcg
    11881 gcaatttcat ccacatgctg ttttgtattg gcaaatacga ttgctaaata aggatgacca
    11941 attgtcagca gttgataaat catttgattc tcatctttac cttttgttga aatcagccaa
    12001 ttttcaatgt cttcagaaat aactgctttt ggtttgattt cttcaatgat tggattttcc
    12061 atgtattttc gtaagaatgg acgaagtttt tctggaatag tggcagaaaa aactaaaaac
    12121 tgcagctttt ctggtaatcg acttgcaatt tggtctactt catttaaaaa acccatatcc
    12181 aatgtcatat ccgcttcatc gacaacaaat gcaaatgcgg tgtgaatctt cagcgcttgt
    12241 tcattcatca tgtctaatat tctaccaggc gtgcctacta caacctgtgg ctgttggtga
    12301 tgcagacgat ttaattgacg ctgtttgtct gtaccgccga caaaattcga gacacggatc
    12361 tccggtgtag aaaatttagc gatttgtgta gctgcttcat agatctgtgc agctagttcc
    12421 ctacttggag ctgtaataac aacttgtact tcctcttttt ctgcattgat tttttcaaac
    12481 aaaggcaaca aaaacgtatg tgtttttcct gtaccagttt gagattgccc aatgatattt
    12541 tttccttttg caattaaagg aatcaaacgt tcctggacct ctgtgggctg tttgaagccc
    12601 tttgcggtta acgcttcatt gataaacggt ttgaagcgga atatatcaaa attattcatt
    12661 cgtgcacctc ttctctttaa attatcacga ctttggcatt ataacacaga caaagcgttt
    12721 ttgctcagct aatttcgaga atcatcatcc tttgtttggc caatcgtgct aacagtatat
    12781 gtgtgactag ctttttcata aaacagaatc cgtttttttc tcaaaaatga tagcaagata
    12841 tttaaaataa aaataccttg tagtaacaag acaccgagag aaatgaatac tgcactcatc
    12901 tgcaacaccg tattcgttgt cgaattcatg atttgctgaa gcaactgccc caaccagctt
    12961 aaaccataaa aaagaaagta cagatacccg tagcggatca tcaatgcttt taaagaaatt
    13021 cgttcgcggt cttcttccac caaacggata cgcacaattt ttttgccaag tgtccatcca
    13081 ttggttataa acggtacaat cataaagtat acccctactt ctaataaaag ccaccagata
    13141 tgagaagtaa ctgcccctga atcgaacgca attttaaaga aaatcgccgc taccaaatta
    13201 ataagagata aaaatgtcca gtcgatcaaa taagcgacca agcgtcgagt caatgagaca
    13261 gtctttcctt tttcataaga aagttcatcc attcgtcttc ttgtaggaag cataaatgta
    13321 aataacggcg taacgacaaa accaatgatt ccacccaatg tatttgtcac taaatcattc
    13381 acatcaaata agcggtatgg tcttgggtag ataaaataaa gtccacttag ctgcgtcaat
    13441 tcaaaaaata gagaaaggca gaaactagca atcatcgttt tcataaaaga aagccggaaa
    13501 taatatctta aataaatgcc aaatggtatg agtaataaaa cattaaaagc tggttccaaa
    13561 aatacactct gcttcattgc cggcaggtag gtactaggtt cagacaagac caataccgtg
    13621 ttgtctctga aatgttgcca tgaagcaaat aaatgcagct ccatctttgg ccctgtcatt
    13681 tgggcaaccg tctcctttgc cggaagtggt aagatcacca aaaaatacgc acacaataaa
    13741 taaaaaacga aagaatacaa aataactgcc cttgttacta gaaatgttcc atactttcga
    13801 tattcataaa taaaaaaaac agatgagatc gctaaggcta aaaatggaaa aacaatcagc
    13861 gcaaaacgaa tcggttcgat ataacttgcc atatgactcc tccttgattc tttgtatctc
    13921 tcttatctta gcagtccttt gctgtttcaa gcaagtaacg aggctattat ttctcatttc
    13981 tttcaaagta aagtaagata aacacaaagg gggtgtcgag atgctgaata tgtactttgt
    14041 ttttggcgta cctatttttt tactgttcct ttatgctacg atcgcttatg taagaaaaag
    14101 aactacaatt cattatttag gatttattct cctgattatt tcgggtttca tgctagtctt
    14161 caatcttcag acttggcagc aagcacttct tgaaatggat aaaatgactc cacatgcttt
    14221 gtctaaagtg cttggctacc ctgtctatct gatttggctt cccattttca tttctggttg
    14281 cttggtttta ttaaatattt atcgcggcgt acgccgaata gttcagttac gcaaatcaaa
    14341 gtaaaagaca gaaatcggtt gagcccgatt tctgtctttt actttgctat ttcatcgtag
    14401 acaaaacgac atttacgtga tcaaaattaa cgagtacttt tgacatttct tccacgtact
    14461 cctcatttaa ataaaatatt ttgtttggca gagaagtgca gttctaataa aattcgaatg
    14521 ttttagctat ctacgtttta aatagagata aatcgggata ccagcaagtg tcaatactat
    14581 ccccatcgaa gctaaaaagg tctgagtgat aagcgtgctt gatacgataa aagtccctcc
    14641 aatgattgct actagtggaa taaacggata cattggaact ttgtatggtc gaaacaaatc
    14701 aggttctttt ttacgaagaa gaatgacacc aacaaagacc atcgtataaa aaatccaaat
    14761 gacaaagatc agcatatccg ttagtgtatc aaaaccaccg atcatcatca tgccaatagc
    14821 gatgactagc tctaagatac ctgcggctac aggaacttta gttttatcat gaagtttagc
    14881 aaagagcttg ctaaaaggca ggttgttttc ttttgccata acataaggga gacgcattcc
    14941 agtaagggta tagccattga tggttccata aacagaaatc aagataccaa ctgtgaccag
    15001 tcttccaccg aagccgccaa aaatcatttt tgcaacgtca cttgccgcat tgctgtttcc
    15061 tgcgactccg tcgatagatg cggttcttaa gaacaccgca tttactaata aataaacgat
    15121 catgattccg atgattccta aagaaattgc ttttggcaaa tctttcgccg gctttttcaa
    15181 ttctcccgaa atatttccta cgtgtatcca accgtcgtat gcgaacatcg ttgctagcaa
    15241 gcccgctccc aaggctgaga aaaatgaaag attttctcct gcttggatcg gaaacagttg
    15301 gaagtctact ccttcttgtc ggaacaaacc gaaaatgacg atcacaaaca aaggaataag
    15361 cttgcagaca agcgtaatcg actgaaaagc ccctccagct ttagaaccta aaaaattgat
    15421 caacaggatc gatacagcag ctgtaacagc tacgggaaca atcattgact gggaaagacc
    15481 aaatagattg acgaactgtg taccgaaaat gatcgacaag gctgctacat ttgctggaaa
    15541 ataaatcacg acttgcgccc agcccaataa aaaagctgct gtatttccgt aaatccgttc
    15601 gatatattta atcatccctc ctgtttctgg aatagcagct gccagttcag ctcctgttag
    15661 tcctgcacag acagaaatca taccaccgag aaaccaagaa aacatatgca aactggcgga
    15721 tcctgtaact tctgctacgc ttgcggcttt aaaaaatacc cctgcaccga tcactgtgcc
    15781 cataacagtc gataaagcag taaaaaatcc catcgtacgt tttaattcat tttctatttt
    15841 tacttctttt actggcgtgg cttgatattc ttccatccct tgtccccttt cataccatag
    15901 ccaatcccaa aagtataaaa aactttttgg atgtggttct tgttttatac cgtctctacc
    15961 aatttttctt tcagtttttc caacatatcc tttgtcatca catctaagtc gtatttcggc
    16021 gaaaagcccc attcctcttg cgcacaagaa acatctagtg aatttggcca agattctgct
    16081 attgactgac gtacaggatc cacatcataa tccatttcaa aatctggtat gattttttga
    16141 atagatgctt tgatctcttc cgggtcaaaa gacatcgccg atatattgaa ggcatttcga
    16201 tgaaccaatc gatcaggatc tgcattcatg agttttataa tggcatcgat cgcatctggc
    16261 atatacatca tatccattga tgtaccttga tcaatgaaac tagtgtactt tccttctttg
    16321 attgctgaat aataaatatc gactgcgtag tctgtcgtac cgcctcctgg caaggttgca
    16381 taagaaatca atccagggaa acggacacca cgagtatcta cgccatattt cacataataa
    16441 taatcacata gtagttctcc agctaccttc gttaccccat acatggttgt aggacgctga
    16501 agagtatctt gtggggtatg atcttttgga gtatttggac caaacgcgcc gattgaactt
    16561 ggcgtaaaaa atttcagctg gaattctcta gcgacttcca atgcattgac cagcccagtc
    16621 acgttcaaat tccaggctaa ttgtggtttt gcttctgcaa cagctgacaa aagagcagcc
    16681 aaatgaatca atgtgtccac ttgatattgt tctgctatgc tgcgcatttt ttcgtaatcc
    16741 attacatcta ggatctcaaa gatatcgttt ttctcaatga cttcaggcat gcgtatatct
    16801 gtcgcaatga cattttcact gcctagttct tgacgtagcc gtgtgatcaa ctctgaacca
    16861 atttgtccta aacatcctgt caccaatact tttttcatga tgatcctccc tgatcttttt
    16921 tactactatc ttcagatgat taacaatata tgcgtgaaca tgttctctaa ttttaaatag
    16981 acgaaaaaac taaatttagt taaataattt ccatttcttt tcctattttt tgataaactg
    17041 ctactgcttc atcaagcatt tccctcgtat gttccgcagt cggcatattt ctgatcctgc
    17101 ctgtcccaag aggcacagtt ggataaacga ttggtttagc ataaatccca tgttcgatca
    17161 gcttcttaga gaaagtctga gtcacttttt cgtctcccaa aatgactgga gtaataggtg
    17221 tttcagacat accgatgtta tagcctactt ttttcaattc ttctttaaaa taattagcat
    17281 tttcccaaac tttttcgaca agttccggat gttcttgcat caaagtaatc gaagccaatg
    17341 cagctgcagc tgcacctgga gttagcgaag tagaaaatag gaatggccga cttctagctt
    17401 tcaaccagtc gattagtgtt ttcgatccag ctacataacc cccaacaaca cctatagctt
    17461 tcgataacgt gcccatttgc atatcgattt tgtcgcttaa tccaaaatgc ttgacggttc
    17521 ctgcaccttt tccagttacg cctgagccgt gcgcatcatc gacgtaggtg atcagtccat
    17581 atttttcaac aatcggaata atttccggca atcgtgcaat gtctccatcc atggagaaaa
    17641 caccatctgt gatatacatg atttttttat atttcttact ttctatcgct tcctttgctt
    17701 ttttctcaag gtctttcata tcttgatgtt taattcggat gatttttgct ccggataagc
    17761 ggcaaccgtc aataatagat gcatggttta attcatccga taagatagca tcttcttttg
    17821 tcattactgc agagatagcg cccatattgc agttgaatcc agattgaaag gcgattgctg
    17881 cttctgtccc tttgaattct gcgatttttt cttctaattg ctgatggatt ttcagcgaac
    17941 cattgatcgt tcggactgct cctgctccta caccataagt ttcggttgct tcaatacatg
    18001 cttttttcaa ttcttcgcga ttggcaaatc caagataatt gttggatgct agattgatca
    18061 tcttttttcc gtctacgatg atacaagcac cattttcact ttctagtatg tcgatcgtat
    18121 tgtataaccc cttttctttc agttgatcca attctttctc taaatacatt gttagttctt
    18181 tggaagacat tgtctccacc tccatattca tctgtcttgc agaacatacc aaacatatat
    18241 cgtgtcattc atctacaaag caagtatgct atatttttga taacgcttac actgattatg
    18301 agaattattt aaccacattt tttatttaca gtatcttttt actttcaaat gacttatata
    18361 gtatgaacaa ttacctttta gataatgatc tattttactc tacctgggca gataaataca
    18421 acacaaccat tgatagaagc cctttcgtta aaatcaattg tattctgaat atttaattta
    18481 tttattgcat agaccaataa aaatacataa aatttccatc ttcgtttatc agcaattttt
    18541 aattttactc tctgaatatt tcttaaaagt tttacttgtt gaattcgaaa aaaggactgt
    18601 gacaaaagtc ttgtcacagt cctattcttc cgaataaacg gtgttctcct gccaattccg
    18661 aatcacaagt tacaatattc atcaaacacg agaagctatt ttttgaaatt gttccttgtg
    18721 actcggagct gaacggcagg ctcacgacct cttggtaaac agtgttccag aagtaacttt
    18781 tacggaaaat gatccgaaat atcttggagc tgaccacttc tgtcacaacc tccatcattt
    18841 tttgttattt ttgatccaat tgtctggagt aaaattttca tcgtcaatag accattccag
    18901 aaaaaattct tctaagaatt cacgcatgaa ggtttctctt cgtttggctt cttcatatcc
    18961 gtaatcagta ttcattttat cagacagcaa gaacagtttt tcataaaaat gattgatcac
    19021 tgtcgtgcct tttcggtaat tttctttgtc cttgaatgtt tgcggataca gttcaggatc
    19081 aaaaatcggg tgcccatgtc caccgccgaa ataagcagta cgcaaaatgc cgattgctcc
    19141 taatgcttcg atacgatcag cgtcttgaac gatcttacct tccagagata gttcggccgc
    19201 ttcagagaga tttttagaaa aggacatatt ttcgataatg gaaaaa
//
LOCUS       DAGCXG010000042        18869 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGCXG010000042 DAGCXG010000000
VERSION     DAGCXG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.043"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(93..1391)
                     /gene="eno"
                     /locus_tag="MB022_001528"
     CDS             complement(93..1391)
                     /gene="eno"
                     /locus_tag="MB022_001528"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBT4466530.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1507..2262)
                     /locus_tag="MB022_001529"
     CDS             complement(1507..2262)
                     /locus_tag="MB022_001529"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBT4466531.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2366..3556)
                     /locus_tag="MB022_001530"
     CDS             complement(2366..3556)
                     /locus_tag="MB022_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBT4466532.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3666..4667)
                     /gene="gap"
                     /locus_tag="MB022_001531"
     CDS             complement(3666..4667)
                     /gene="gap"
                     /locus_tag="MB022_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4466533.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4708..5799)
                     /locus_tag="MB022_001532"
     CDS             complement(4708..5799)
                     /locus_tag="MB022_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBT4466534.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5948..7282)
                     /gene="rpoN"
                     /locus_tag="MB022_001533"
     CDS             complement(5948..7282)
                     /gene="rpoN"
                     /locus_tag="MB022_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBT4466535.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7342..7413
                     /locus_tag="MB022_001534"
     tRNA            7342..7413
                     /locus_tag="MB022_001534"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7375..7377,aa:Arg,seq:ccg)
     gene            complement(7605..8489)
                     /locus_tag="MB022_001535"
     CDS             complement(7605..8489)
                     /locus_tag="MB022_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4466536.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8576..9475)
                     /locus_tag="MB022_001536"
     CDS             complement(8576..9475)
                     /locus_tag="MB022_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBT4466537.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9492..9998)
                     /locus_tag="MB022_001537"
     CDS             complement(9492..9998)
                     /locus_tag="MB022_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBT4466538.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10187..10807)
                     /locus_tag="MB022_001538"
     CDS             complement(10187..10807)
                     /locus_tag="MB022_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBT4466539.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10883..11320)
                     /locus_tag="MB022_001539"
     CDS             complement(10883..11320)
                     /locus_tag="MB022_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBT4466540.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11483..12313
                     /locus_tag="MB022_001540"
     CDS             11483..12313
                     /locus_tag="MB022_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBT4466541.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12297..13664
                     /locus_tag="MB022_001541"
     CDS             12297..13664
                     /locus_tag="MB022_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4466542.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13683..14492
                     /gene="yidA"
                     /locus_tag="MB022_001542"
     CDS             13683..14492
                     /gene="yidA"
                     /locus_tag="MB022_001542"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBT4466543.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14535..14978)
                     /locus_tag="MB022_001543"
     CDS             complement(14535..14978)
                     /locus_tag="MB022_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBT4466544.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15112..15483
                     /locus_tag="MB022_001544"
     CDS             15112..15483
                     /locus_tag="MB022_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4466545.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15652..16434)
                     /locus_tag="MB022_001545"
     CDS             complement(15652..16434)
                     /locus_tag="MB022_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBT4466546.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16431..17402)
                     /locus_tag="MB022_001546"
     CDS             complement(16431..17402)
                     /locus_tag="MB022_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4466547.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17494..>18869
                     /locus_tag="MB022_001547"
     CDS             17494..>18869
                     /locus_tag="MB022_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4466548.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEK"
BASE COUNT         5314 a         3883 c         3363 g         6309 t
ORIGIN      
        1 tatttcagca agtagagagt tcgattactt aactaagtga tcatactcga ctatctttca
       61 atagtcgatc atgtatcttc attagaaaag aattatttgt tttttaagtt gtagaaagat
      121 ttcaaacctt tgtattctgc aacttcacca agttggtctt cgatacgtag taattggttg
      181 tatttagcaa tacggtcagt acgtgataga gaaccagttt tgatttggcc agcatttgtt
      241 gcaacagcga tatcagagat tgttgaatct tctgtttcac cagaacggtg agatacaact
      301 gctgtgtagc cagcttcttt agccatttcg atagcttcga aagtttctgt taatgtaccg
      361 atttggttca ctttgataag gattgagtta gcaattcctt tttcgatacc ttcagataat
      421 ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt gaactttgtc gcctaatacg
      481 tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta atccatcttc gattgagatg
      541 attggatatt tagaaactaa ttcttcgtag aatttgatca tttcgtcagt tgttttttcg
      601 ccttcgcctg agtcagctag tacgtaaaca cctttttctt tgtcgtagaa ttctgaagaa
      661 gcagcatcca tagcaagaac aacgtcttta ccaggtacat agccagcttt ttcgattgct
      721 tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt ttggagcaaa tccaccttcg
      781 tcaccaacag aagttgctaa tccgcgagct ttcaagattg aagctaatgc gtggaatact
      841 tcagcaccca tacgtagagc ttctttgaat gttggagcgc caacaggcat gatcatgaat
      901 tcttggaagt cgattgagtt atcagcatgt gatccgccgt tgatgatgtt catcattggt
      961 gttggcaata cttttgtgtt gaatccgcct aggtagtggt ataaaggtac ttctaggtag
     1021 tcagcagctg cacgtgcaac agcgatagaa acacctaaaa tagcgttcgc acctaattta
     1081 cctttgttag gagttccgtc taaagcgatc atagctttgt cgatagccat ttggtcacga
     1141 acatcgtagc caatgattgc ttcagcaatg atgttgttta cgttgtcgac agctttagtt
     1201 acacctttac cgccataacg agctttgtct ccgtcacgta attcaactgc ttcgtattca
     1261 ccagttgaag cacctgaagg aaccattccg cggccaaaag ctccgctttc tgtgtatact
     1321 tctacttcga ttgttgggtt accacgtgag tctaagatct cgcgagcgta aacatcagta
     1381 ataattgaca tgttttgtct ctcctttgag tttgtttttt cctaagggat ttccctcata
     1441 tacgatttta gttaattttg ctcattagtg caaacatttc ttgcattttt cgcaaatttt
     1501 caacaattat tttacagcgt ctaacaatga caaaaatgaa tctgcttgca agcttgcgcc
     1561 accgactaaa gcaccgtcaa cattttcttt tgccatgtat tcagcaatat tttctggttt
     1621 tacagaaccg ccgtattgga tacgaactgc ttctgatact tcttttccat aaagtttttc
     1681 aactgtttga cgtacaactc cacaaatttc gtcagcgatt tgtgcgtcag ctgatttacc
     1741 agtaccgatt gcccagattg gttcataagc aataaccatg tttgacactt gttcagctga
     1801 aagatcagct aaaccagctg taatttgtcc ttcgatccat tcagcagttt tgcctgcttc
     1861 ataggtttct aaagattcgc cgcagcatag gattggtgtc ataccatttg caaagatagc
     1921 ttttgctttt ttattgatgt cttcgtctgt ttcatggaaa tattcacgac gttctgaatg
     1981 tccgatgatc acataatgaa cacctagatc agcaagagct gctggtgaag tttcaccagt
     2041 gaacgcacct gagttttccc agtagcagtt ttgtgctgag atttttagtt ctgtaccttc
     2101 agctgatgtc attaattctt gtaagaataa tgcaggagat ccgatgactg aatcaacttt
     2161 gtcatttgaa gggattttgt tttttactgc ttcagcaaat tcttttgctt ctgatgcagt
     2221 tttgttcatt ttccagttac ctgcgataat aggtttacgc attgaggaac acactcctta
     2281 atttattgaa aaatggctag ggagtcatga aaactccctt caccttttga tcgattactc
     2341 gtcagaattg agcaaacttg gtcgtttatt tatcgttgat tgctgctagt cctggtaatt
     2401 ctttaccttc aagcaattcc aagcttgcgc caccacctgt tgagatgtgt gtgaatttgt
     2461 cagcaaatcc taattgttct gctgctgcag ctgagtctcc gccaccgatg attgttgttg
     2521 cgccttctaa gttagcgatt gcttcacaaa caccgattgt acctttagca aagttactca
     2581 tttcaaatac acccataggt ccgttccaaa caactgtttt tgcaccttgt aatgtatcag
     2641 caaataattt tactgatgct gggccgatgt ctaaagccat caaaccatca ggaatgtctc
     2701 cttcgattac ttgtgtttca acatcatttg aaaattctgg tgcacagaca ttgtcgattg
     2761 gcaagacaag tttgtcgcct gctttttcga ttaattcttt tgctagttcc actttatcag
     2821 cttctactag agagttaccg atcttgattc cttttgcttc gtagaatgtg taagtcattc
     2881 cgccaccgat caagatttta tctgctttag gaatcaagtt ttcgattacg ccgattttat
     2941 cagaaacttt tgcgccacca aggattgcaa tcattggtcg ttttggttct tctactgctt
     3001 caccgatgaa cttgatttct ttttccatca agaatccagc aactgttggg ataccagttg
     3061 aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt accgaaagca tcgttaacaa
     3121 atacatcgcc taatgaagcc cagtatttac ctagttctgt atcatttttg ctttcttttt
     3181 taccgtcaat atcttcaaat cgtgtatttt cgaatacgac aacgtcgccg tcattcatgt
     3241 tgcggattgc ttcttctaat tgttctccgc gagtttcagg tacgaatgtt acttctttac
     3301 ctaataattc gcctagacgt tttgcaactg gagctaaaga ttttccttct ttatcttctt
     3361 ctgtttttac acgtccaagg tgagagaaaa ggattgcttt tccgccttgt tctagaacgt
     3421 agttgattgt tggcaatgct gctttgatac gagtgtcgtc agtgatcacg ccgtctttca
     3481 aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc ttttaaatcg atatctttca
     3541 ctgttttctt agccatttta tttcctcctg aataaactta ttatcaaaaa aagcggaaga
     3601 agcgcgattg cttccccgct tttaaatgta catgtctata atttacactt gtacgtttgt
     3661 ctatcttata agttagcgaa gtattctaaa gtacgtacta attgtgcagt gtatgacatt
     3721 tcgttgtcgt accaagcaac agttttaact aattgtttgt cgccaactgt cattacttta
     3781 gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa cgatatctga agaaacgatt
     3841 tcgtctgtgt tgtatccgta agattcgttt gaagcttctt tcattgctgc attgatttcg
     3901 tcaacagtta cttctttttc aagaactgtt actaattctg ttaatgaacc tgttggtaca
     3961 ggaacacgtt gagcagctcc gtctaattta ccgttcaatt caggaattac taaaccgata
     4021 gctttagcag cacctgttga gttaggaacg atgtttgcag cagcagcgcg tgcacggcgg
     4081 aagtcacctt taggatgagg tccgtctaga gtcatttggt cacctgtgta agcgtggata
     4141 gttgtcatta atccttcaac aacaccaaat ttgtcgttca atgttttagc cataggagct
     4201 aaacagttag tagtacatga agcacctgag ataactgttt ctttaccagt taatgtttcg
     4261 tggtttgtgt tataaacgat tgttggaaca tcgtttccgc caggagcaga gataacaacg
     4321 cgtttagcac cagctgttaa atgtttttca gctgctgatt tagaagtgaa gaaaccagta
     4381 cattctagaa cgatatctac gcctagttcg ccccatggta attcttctgg gttgcggtta
     4441 gctaatactt taacttcttt tccgtttacg ttgaatgaac cttcatgaac ttcaactgtt
     4501 ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt gtgccaacat tttagcatct
     4561 gtcaagtcgt tgattgctac tacttcgatt ccatctacat cttggatacg acggaatgct
     4621 agacgtccaa tacgtccaaa accattaata cctactttaa ctgtcattta agatttcctc
     4681 cttatgaaaa tcaaaaaaat atttatttta aagggttacc ccttttaaaa tctcatttgc
     4741 agcagcttca tcagtgatga gccacgtttg ttttggtgca tttttcatat aagctcgaat
     4801 tgctttagct tttgattttc cgcccgcgat tgccatgatg atcgggactt tttccaaatc
     4861 ttgcaattct atgccgattc tagggacctt ataaacgact tcaccttttt cattgaaaaa
     4921 gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc atgactaatt ctttttcagt
     4981 catcttacga cgtgctgcca tgtgtaatgc acgcccaata ctatgaataa cacaattcgc
     5041 ttcactaatc aagtttaaga cttcttggat agatggctca ttgagtaaag aattgtaagt
     5101 tgccagactt aattgttccg gaacataaag tgcacggtgg ttaccgtttg ctttagcagc
     5161 cattcttgca ctgacagaat ttgcctgtac agttatcgct tcaccaatcc cgcctcttgc
     5221 agggacaaac aggttatgcc gtttcttatt ttccagatta gacaattgtt cggctaccac
     5281 agccatagta gttcctccca tcacagcgat gatattctcg ccttctggaa gtagtcggtc
     5341 tagggcttcg ctcagcgctt cgccgaatgc atcagccact ttggtctgct cttcgctgtt
     5401 tcctgaaaca acgatacagc gttggttacc aaaatattct gccaactgtt gttcggtttg
     5461 atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt tgataaactt cttcgccctt
     5521 agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa ttcagatttt tcaaaagatc
     5581 tgtttctgtt cgcaatactc gttctgtgat ccccatactt tccgataaac ttcgacgtcc
     5641 aatcggctgc atccaataga tatttcgtaa aattttatag cgttcttgca acacttcgat
     5701 aatatctggt gcaacagatt caatcatttt tatttcatcc agcatagcga tctccttagt
     5761 tggacacttt acgtccaatg tagacttaaa acgacccata tacgataaaa aaagaaatta
     5821 actattcgaa tttcacatca ataaagggaa atctcaacct cacgactgta agtctaacag
     5881 ttttgctctc gtttttcaac taaaaagact agaaaaagtg aaatactttt tctagtcttt
     5941 tgactgatta gttatcataa cgttttcttt ttgatgaact cgggattcct aataaatcac
     6001 gatattttgc tactgttcgt cttgagatcg ctatctcatc ttttttcagc aattcaacca
     6061 atttctggtc agacaacggc ttgtttttgt cttcttgatc gactaattcc tgcagctttt
     6121 tcttcgctgt atctgctgaa aggtcttctg tctgttctgt actattcgta ggaattcgtg
     6181 tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt ttccaaatat ttcccgttga
     6241 ctgcacgact gaccgtggat tcatgcacat ctatctcagt tgcaacttcc tttaacgtaa
     6301 gaggcttgat tggacgttcc ttgtcgataa agaaagcttt ttgccgggaa acaatgactt
     6361 gagcaacccg caaaattgta tccccacgtt gtaggatcgt tttcttcaac cattcgaatt
     6421 cctgtttccg ttcttttaaa taattttcag tttctttatc tgcttgttgt gacatttgtt
     6481 tgaagtaact ctcttgaaag cgtatatttg gctgattttt tcggtttgag ataacttgta
     6541 catttttatc gtcgtccact aatacccgga catcaggaat aatgtacaat tcgctgctac
     6601 ggtcgaaaat ccggccaggg cttggactca acgtttgtaa atagtcgaat atctgctgaa
     6661 cagcatgtag atcaacagaa aaacgttgtg cgatttcttt ccattttcgc tccaccagtt
     6721 cctcaaaaca ttcttccaac acgatataag ctaattccgg cgcataatca tctcgttctg
     6781 tttgaagcat gagacattct tgcaaactac gtgcacctac tccagctggt tctaattgct
     6841 gaatcagagt caacgcatcg agcatttgga taggcgtagc tccaatctct tttccggctt
     6901 cttctaaact aatcgtcaaa aatccgttca aatcgatgta ttctacaaga taaagaacga
     6961 ttttcctcaa aaaagtatct cggtaattca aatggacttg ttcgatcaat gaatcaaata
     7021 aagacccttt tgtatcagga atctgattta ggtaatttgt ctcttctcct tcataagaag
     7081 atgaagaagc ttttatgtaa tctggctgcc attccggctc aacaacttcc accaatggat
     7141 tttccattgc ttgattctca acgaatgcca acagctcctc actgttatat tgtaaaactt
     7201 ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg tgtctgctgc tgtttttgtg
     7261 aaaaattttg ctcaaatttc atttttttgt ccaatcccct tgtaattttt tatctgttta
     7321 gttacaatta tcccagtgct tgcgtcctta gtgtagtgga tatcacgtaa gattccggtt
     7381 cttgagacgg gggttcgatt ccctcaggac gcgttctact gctattttag cataatttac
     7441 gaatagttgc ttctttattt ttaagtgaaa actgtccgcc aaagaaaatt ggtggacagt
     7501 tttttgttgt acctgtatag atattgttaa gcctttgcaa gtaaggtagt tatgtgttca
     7561 actacttatt aaaatgtgtt atttattaaa gatacttgtc tcacttactg aactggccga
     7621 tgatcgtttc tgccataata gttctcgaca gccgttggat gccctaataa atccccaact
     7681 gtcgtgatgc gatacccttg ctctttcaga taatcgatca cttgaggaac ggcacgaatc
     7741 gtttcaggat agatatcatg gaataaaata attgccccat cgtatactgt tgcttggatc
     7801 ttctgcaaaa tcaaatcagg atttcttgtc ttccaatctt ctgaatcaac agaccattcg
     7861 atgatcggtc gctggatgat cgttgctcct acattcgtga cagaaccata gggcggacga
     7921 acaaaagtgg gggttttgcc agtgactttt ttgatctcag tgtctgcttt cgtcacttcc
     7981 tccgtcatcg actgggcaga aagagtggcc agattctgat gatcgtatgt gtgattgcca
     8041 atctcgtgcc ctgcttcagc tgtctgcttc acgatttcag gatgctgcgc cgtattttct
     8101 cctaagacga aaaaagtggc atgcacgttt ttttctttta agattcgcag aagctgcggt
     8161 gttgtggtcg ctcctggtcc atcatcaaag ctgattgcga cgcttttgac agccggatct
     8221 cggctcgttt ttgtactgtc ttgtgttgat tgtgatgact gactttgtac cttttccgct
     8281 gattcttgaa tggcttcttt gacgttagct gtttcagttg attggctttt aatggttgat
     8341 tgttctgatt gtttgaaaga ttgtgacgaa tgctccgaag agtgtgtttg tccttttact
     8401 tcggtgatca aggaggccac cgaagtaatt tcgaaaaaca aaagcacgga taaagcaata
     8461 atactgataa ctaaataatt tcttttcact atatctcttc tttctctttt tctcttctta
     8521 ttatttactt gtacttactt gaaaattgaa actgctagtc gcaactactt ctcgtctaat
     8581 gatccaaata atcttccgat gccaaaatcg tttttgtcac ggacttagga atgacaaatt
     8641 ctggtgtacc catataacca ggagcaattt cgtattgaga aaaagtcaaa actaattgat
     8701 gctctttgtt caaataaaaa cttcgtgtag gcgtaacaag tgttggtttt tctatcgtcc
     8761 cttcttgaat atcttgctca gtccaatagt acttagatgg atcttccttg acttgttcgg
     8821 ccatctgccg tttgacttct tttgtcaaaa cagatagata ggtgtcatct ttgaataaaa
     8881 gtggaagtga gagctggaca ctgtttttct tgtcgattgt gtcgtatctt ttttcaacat
     8941 ggctatctgc tgctgtttgg gtgatctgcc tttcaatcac gagaaaccgg ttatcgtcta
     9001 cgattttctg ataatctccc aaaacttggt aatgatccgt ttcaaaattt ccatattctt
     9061 tttcagcttt ttcaaaatct gcttgtcctt cgcgaaagta tttttgattt aatccatgaa
     9121 ttgttttatt ttctttctca ttttgtgtat tgatcttcgg tacctgaata tcgatttttt
     9181 cagatcgatc agtaaagcgt tcgcctaaga tcaattcaat gactgatcct aagatgggta
     9241 aatcttctgc aaagcttcga acttgactat taaaaagaag gctacttgtt gtaatcatta
     9301 cgatagccaa agatactccg aacccttgga gacgtctctt tctccgcttt gcttttataa
     9361 atgattgctc ttcgataata aaacgctggc ggatctcctc tttaagccct ttgggaattg
     9421 cttgttcttt ataagttttc tttagttttt ttaaattttc tttttgggaa tacatggcac
     9481 tcttctctcc ttcattctag gatttgacgt aaagcattca atgagcggta cattttcgtt
     9541 ttaactgtgt tttcattcat atccaagata gtcgcaattt ctttcaaacg aaatccttca
     9601 aaaaagtaga ggatcagaat ctctttttcc ggactgtcta ttttatctaa aatcatatgg
     9661 agctctgcaa cggaagtttc taaaggtaac tgttggggga tatcttgaat ttcaaataag
     9721 tccacaggcg tccttttttg tctgcgccaa ctatctaatg ccgtgcggat caatatccga
     9781 taaaaccatg cactaaaatg ctcgatctct ttttcttttt caaaatagtt cagggcttta
     9841 tgaaaactat cttgcatcac atctaaggcc tcttctttat catgtacgta attcatcgtg
     9901 atacggaaca ttttttgata atcagtttcg atgagtccat tcagcaggtg acggtatttt
     9961 tcgatctgcc gttttttctt atatccagta aaaatcattc tccactcctt tctgcctcac
    10021 ttaatatgac gtacaaggtg cgctaaaagt ttcaattttt ttatttttta tgtatttact
    10081 tgggattttt atatacaaaa aagaaggtgt gacaaaaatc gtgtcacacc tccttattct
    10141 gataggctga tcgtctctgt cacaacctct cgtaagaaaa taatgtttat tctttttcag
    10201 agtcagaaaa ttcttcctct tcataatctt catcatcatc gtcttcgtct atgatactta
    10261 aatcttcttc gatatttcct ggcaagtctt cttcatcatc tgtcgggtca tctgctccga
    10321 tttcttgcaa gtcagaatta taagcagcga tttcttcatc ttcatcatca tcgtcatcaa
    10381 acagatcatc gtcatcttct tctgtcagat ctgtatcttc tgggtcatcg tcattgtaat
    10441 cgatcgcgtc ttcatcatca ttgataaagg cgttgacttt cttgcgttta cggcgacgtg
    10501 gagtatcttc atcctcttcg tccattccat ggtttacttc ttcatcgatc gaatcgatag
    10561 cataccatga acgcaagccc caacgattat cccctaatga gataaagctg ccgtcaatat
    10621 tcaagtcagt ataaaattgt gcgagtgaat cacgaatttc actgtctgat ttcccaagat
    10681 agttttggat ctggttagct aaatcagaaa aatccatgac atcgccacgt tgttctaaga
    10741 tcgcgtgtgc tacttcgatc attgataatt cgcttttgtt tgcaccttca aatacactaa
    10801 tttccaatca ggacacgtcc tttcacagtc tactccttat catacaaaat cagacacaaa
    10861 aaatcaaact tttcttttat atttacgctg taaaatccaa ggtcaaataa tattcgccga
    10921 ttttttccgg tcccatgaac agatcataat cgattcgaac cttccctgct gtgggacggt
    10981 ctttcaaact aacatgcagt tctttcgtaa aagtagcaat ctggatcata ccgtatggtg
    11041 ttcgatagga tgtttctaac cgttctttat agccaaattt caaacgcatc cgcatttctc
    11101 ctgcacggat cagctgtaca tgaccatctg gaactaattt gatcgttaca ggaatctcat
    11161 cgcccgtctc ctcctgaatc tctttataac gtatgtacag cgtatcgcca attgtcacca
    11221 tttgcccttt caaatcaaaa taaaaatctt gcacttcccc ttgctgtttt acttttgtac
    11281 gcaatttgat tgaaatcggt gttccttgtg ataattccat tattcattct cctttacttc
    11341 tcatagctac attgttccta gtttcttttg ttttgtcaac caacttgtcc atttttacag
    11401 aaaacctttt ttccaaaaca actctttgat tttagagtat aatattgtag aaaatatgaa
    11461 tttatacaaa ggagccggtt gaatgaaccc tgcgattcga ctagatcagc acatttatca
    11521 aaaaaacgac ttttcccctt ttgcattaac agatatatta acggaatatt ctaaagaaca
    11581 ccagcaaatc tttttacatt tctggcaata tgactgcaca gtcatcttag gtatgaagga
    11641 tgttcgaact ccttttctaa gtgacgggat ccaaacactg gaagatgcag gttacgctcc
    11701 agtgatcaga aattcaggtg gattagggat cgtatcagat gaaggaattt tgaatatttc
    11761 tttgattttc ccccaaagtg acacaaaaaa aataaccata gatgacgctt acgagcaaat
    11821 gctcctattg actaaatacg cttttccaaa ggcaccgata caagcattcg aaatcaccca
    11881 ttcttattgt cctggaactt atgatttaag catccatggc aaaaaatttg ccggtatcgc
    11941 tcagcgtcgg atcaaaaatg ggatttctgt tatgatgtac ttaagtgtca acggtaatca
    12001 ggaaaaacgt gggcaagtca tgaagcgatt ttatcaatct gctttaaaag aagactttgg
    12061 tacaaatggc tatcctgcag tggatcctgc tacaatggca acacttgaag aactgcttga
    12121 ccaacccctt tttgtaaaag aagtcaaaga agcatttgca aaagcttttg aaaagctgta
    12181 ttctgcaagt gatttacagg attctaccga ctggctaaaa aaagaaatcc agctggagca
    12241 atggaacaac caaatccaga gaatggaaca acgtaataac ataaaggagc tacattatga
    12301 caacccctta taaatatcaa atgctgccta tggaaaaagt ttttcgtgat ccagtccata
    12361 actatatcca tgtccagcat caagtaatct tagatttgat caattcgaaa gaagtccaac
    12421 gtctacgacg cattaaacaa ttaggcactt cttctttcac ttttcacgga gcagaacata
    12481 gccgttttac tcattcatta ggcgtttatg agatttcaag acgaatctgc gatatctttt
    12541 ctcgaaattt ttcaaaagaa aaaataggca acggaggctg ggatgatcag gaacggctgg
    12601 tgactttatg tgcggcactg ttgcatgatg tcggtcatgg tccttactct catacatttg
    12661 aacatatctt ccatacgaat catgaagcca tcactgtaga gatcatcact tctccagaaa
    12721 cagaagtgta tcagattctt aaccgagtag aagaaggttt tcctgaaaag gtagctagtg
    12781 tgatccaaaa aacttacccc aacccacaag ttgtccaaat gatttctagt cagatcgatg
    12841 cagatcgaat ggactatttg cttcgggatg cttatttcac tggaacagaa tatggaacat
    12901 ttgatcttac ccgaatatta cgtgtgattc gtccttatga aggcgggatc gctttttcca
    12961 tgagtggcat gcatgcagtg gaagattata tcgttagccg atatcaaatg tatgtgcaag
    13021 tgtacttcca tcctgtttcc cgtggtatgg aagtaatttt ggagcatctg cttcatcgcg
    13081 cacacgagtt gtatcaagaa gatgccagca gttttgaact ccattctcaa ttacttctgc
    13141 catttcttga cggaagcttt actttagaag aatacctcaa acttgatgat ggggtcttgg
    13201 gcacttactt tactcaatgg tctgaatctg ctgatcctat tttaaatgat ttagctaaac
    13261 gttttttgaa tcgtaaacca ctgaaatctg ctactttttc tggcaaccgg gattcaaaat
    13321 tagtccaaga actcactttg ttagtagaaa aggtcggata taatcctgtt tattacactg
    13381 ctgtgaattc tagctacgat ctaccttatg atttttatcg cccagaacaa ggcagacatc
    13441 gcacacaaat cgagattttg cgaaatgacg gaactttgat agaattatca caggttagtc
    13501 agcttgttgc tgcattagcg ggacaagaac aaggagatga gcgatttttc tttccaaaag
    13561 aaatggttga tccgtccttg cgtgatcatt atgatttatt tgacgaaact tatcaagaat
    13621 ttgccagcca tatccgtaat ggcgcattga ttgaaataaa ttaaaatata ttggaggaaa
    13681 ttatgtctat caaactagtc gctatcgaca tcgatggtac tttattaaat gagaaaagag
    13741 aagtaacaaa agaagtaaaa gaagcgattg ccgctgctgt tgcaaaaggt gtttcgatcg
    13801 ttttatgcac tggccgtccc ttaccaggtg tccaggaaca gttaaacgag ttgaaccttt
    13861 ttcaagataa tgattacgtt attacttata acggggcact cgtacaacaa acgaaatcag
    13921 gtaaaatcat cgcccgtcac ggactgacac atgaagattt cctagagatc gaagtaatgg
    13981 cacgccgtgt cggcagtcat ctccattcga tcgatgacaa agcgatctac acgcctaatc
    14041 gagatatcag cgcttatacg atccacgaag caagtttagt caagatgccg ctaaaatatc
    14101 gaacacctga agaaatgaca ccagacatga acatcgtcaa aatgatgatg atcgacgaac
    14161 cagaaatttt agatgcagca attgcgcgtt taccgcaaac tttccgtgat aaatacacaa
    14221 cggtcaaaag tgcgccctat tacttcgaag tactaaataa agaagcaagt aaaggtgcgg
    14281 cagttgcgaa tctcgcacag caccttggta ttgatcaaga tgaaatcatg gctatcggag
    14341 ataacgagaa cgacttatcc atgattgaat atgctggtct aggagtagca atgggaaatg
    14401 ccgtttctct tgtcaaagaa gcagcaaatg tcatcacttc atcaaacgac gagcacggtg
    14461 tagctgaagc aatcaaaaaa tatgtactgt aataaataaa aaaactgaga gaaagctgat
    14521 ctactcgctt tctttcagtt tttatttttt tcgatccatc tctcgactgc ccatcggtgg
    14581 ctaccacgtt tcaatttcac aagcgcttct tgagggctca cccaccaaat tttattggtc
    14641 ttttcagttg gttctcctat acgcttccaa gaatcagcaa caaagaaata tcctggatta
    14701 taataataag ttgcacgata attcgagtaa aaatactcat cagcttctcc cagataagaa
    14761 gaaatttctg ccacaattcc catttcttct atcagctcac ggacaatcgc ttcttctttc
    14821 gtctcttctc cttcaatttc cccgccagga agaaaaaaag caccatttgg agcctcgatt
    14881 actgcaatct gctgatctct ttctatgaca atatatgctg cataacgggt ttgataatca
    14941 gcaaattgct ttttttcacc aaaaattggt ttatccatga atagtttcca ccttaacagt
    15001 tttattttct atccatttta catgagaaat ttctcatgaa caagcacaaa tcaactattt
    15061 ccttacttct ttttgttatg attgagtaaa aggaaaagga gagatgaatc tatgaaaatg
    15121 gctcacacat gtgttcgagt gaaagatctt gaagcatcaa tcgaatttta tcaaaaggca
    15181 tttggttttg aagaaagccg ccgccgtgat tttccagaaa acaaatttac acttgtttat
    15241 ttgacacttc ctggggatga ctatgaatta gagttgactt acaactatga tcatggtgcg
    15301 tatgatctag gagacggcta tggtcatatc gctatagcag ctgatgatct agaaaaatta
    15361 cacgagcaac ataaagctgc tggtcttgag attactgact taaaaggatt acctggcact
    15421 gcgccttctt actactttgt cattgaccct gatggttata aaatcgaagt catccgagga
    15481 tagttagtat ggggcatttt actcttgatt gaataaaaaa caaacaccaa taattaaaat
    15541 gagacgcaaa cttttcatta aattgaaaaa gtttgcgtct ttttttaaca accattgtag
    15601 ctataaggtt gccactctcc accaatttat ttttaattca tttgaaagaa atcaaataaa
    15661 cgaggtacct ttagaaaaca aaactgcttt tcctcctatt atcacacgat cttcttttaa
    15721 ttcacattct agatccccgc cccttgatga catttgataa gctgacaatt ttgttttatt
    15781 cattctttct gcccaataag gaataagatt agaatgtgct gatccacata ctggatcttc
    15841 attgattgtt aattttggaa agaaggttct agaaacaaag tctttttgca cactttctgc
    15901 tgtcacgatg actcctattc ctaattcaaa atttttgata gctgtaaaat ctggctgcaa
    15961 tttagccacc gttttttcat cttctaatac gaaaaataaa tcacgtgcta agtaggcttc
    16021 tttaatctta gccccaatcg cttcttcata ttcagacaat atcggcactt ttttcggcat
    16081 aatactagga aaatccatgt aataataatc tttaattctt gtcacaaata gattaccgct
    16141 ttgagtagaa aatttaatcg tttcatcagg tattttgtaa tagttaaata aaacaaatgc
    16201 tgttgctaat gtcgcgtgac cacaaagatc gatttctcga tctggcgtaa accatctaag
    16261 ctcaaattct tggtttttct tcactgtaaa tgctgtttca gataaattgt tttcaatggc
    16321 aattttttgc atcgtacctt ctggcagcca ttcttctagc acataaaccg ctgctggatt
    16381 tcctttgaac acctcatctg caaatgcatc aactacgtaa taatctaagt tcattttaaa
    16441 gcccctttct ctaatttgtt ataaatatat tctgcacaac aaagatcttc aatagctaga
    16501 ccaactgcgt cgaaaatcgt cggcctcgat tctcctttat aaaatgtttt tttctcagaa
    16561 actaattcgc ctaatgtacc aacaatacaa gattcctcta aatctccttc tgccatggct
    16621 ataagcaggt ccccgctttc tgttaaggca gctgagtaat catctacata gatttgagaa
    16681 gtttccatca acgatgtact tatctctctt gtatcaggag taaatgtgcc cacagcattg
    16741 atatgtgcgt cttcccttac ccactctttt tgaagaaatg cctctttact agaggtcaac
    16801 gtacaaatga tttccgctgg acgtactgca tcttctactg atattgcatt tataaacgtt
    16861 attttaggat actgtttttt agctttagcg ataaaagtat ttcgtctttc ttctaaaata
    16921 tcgtaaacaa acactgtagt gatgggacga atggtagaaa tcgcagtcaa atgcgacaat
    16981 gcttgttgac cagctccaat taaagcaagt gtttctgcat gcggatttgc taagtaatct
    17041 gttgccagcg cagatacagc agctgttcga atccaggtga ctgcatttgc gtttatcaat
    17101 gctttgggta gtccgttttg agcatcaaac aagaaaatct ctcctaaatg agaaggtaaa
    17161 tgatactgat gattatccgg aaaagcggta atgacttttg tgccaaaata tctgttctcc
    17221 cccaaataag caggcatcat cgcaaacata tttttgcctt ttccgtcagg taaaatttcc
    17281 accaatcgag gattttggct gattttacca ctttcataat caaaaaaaca ttttttcatt
    17341 acttgaatcg cttcttcaaa actaaaagaa gctactactt cctcaaaatt aataattttc
    17401 atagacaatt ccctcctcac aaaagattaa cttctaaaat caatacaatc aatggtaaaa
    17461 ttgtatggta tacaatttta aggagtggag aaaatgccta ttaattctta tgacaattac
    17521 ccgttaactt ggcgaccaga caaacaaaaa ttgaagcgtc cttattatcg atcgttgatt
    17581 caacaattag aacaagatat tttaaagaat aaattacaga aaaacactca gttaccctcc
    17641 caaagagagc tagctgatta tttagattta aattttacta ccgttggcaa ggcttataaa
    17701 tatggaattg agaaaggttt gctgtatacg aatattggaa gtggcacatt tatttctccc
    17761 aatgtctttg agtccatcac catctctacc aatgaagtaa ccgaacatat cattgatttt
    17821 ggtctggtta gttcttttga agaatgtaat aaattcgttg tcccgtttat tcaatcaact
    17881 agtaataacc caaatcttgt tggtttgctt aattaccaag atcccaaagg cacaaatttt
    17941 caattgacta ctgcagcaaa ttggttaaaa actcaaggag tcattccgtc cttagaatct
    18001 ctatctattg tttccggtgt tcaaaatgga ttggctatca cactttctgc acttttctct
    18061 ccaggtgata gaatagcaac agaccgatat acatacgcta atttcattga attggctcag
    18121 ttatatcatt tagagatcgt tccaattgat tttgacgagc aaggaatgcg acctgatata
    18181 ttgattcagg aatgtagaaa aaagaagatt catggtgtct ttcttatgcc ttcttgtaat
    18241 aatcctatag ggtttcaaat ctctcacaaa agacgagagc aattagccga agtgattgaa
    18301 aaagaaggtc tatgggttat tgaagatgat atccattcat ttttaacaac ttactatcaa
    18361 catacgatca ttcctccttt ccaaaattta ttacctaacc aaacaatcta cttagctgga
    18421 atgacaaaat ttatttgttc tggattgcga gttgcttatc ttgtagttcc agtacatgtc
    18481 caaagcaaga tagaacaagc aatttttaat ataaatgtga aaacttctgg attagatgtg
    18541 gaagtcatca ctcaaatcct ccattcaaat gaagcaaata ggatcttaga aactaaatta
    18601 agtcttacaa aaagagccaa tcagcttttt gattctcttt tcaaagtaga aaaaccaacg
    18661 aatcgtgtcc cttactatcg aattcttcca gttgatcctg agattgaccc taagataatt
    18721 gagaatgact ttcttgctaa cggtatccga ctttatcatt ccagacgctt tactacacgt
    18781 gcgcaaccag atgcattcgt tcgaatttcc ctatcctcta ataaccttga aattttagct
    18841 tctggactaa gtatcgtaaa agaaaaaat
//
LOCUS       DAGCXG010000043        18866 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGCXG010000043 DAGCXG010000000
VERSION     DAGCXG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.044"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..156)
                     /locus_tag="MB022_001548"
     CDS             complement(<1..156)
                     /locus_tag="MB022_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466549.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAK"
     gene            356..622
                     /locus_tag="MB022_001549"
     CDS             356..622
                     /locus_tag="MB022_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466550.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            635..793
                     /locus_tag="MB022_001550"
     CDS             635..793
                     /locus_tag="MB022_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466551.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(790..1020)
                     /locus_tag="MB022_001551"
     CDS             complement(790..1020)
                     /locus_tag="MB022_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBT4466552.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            1092..1247
                     /locus_tag="MB022_001552"
     CDS             1092..1247
                     /locus_tag="MB022_001552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466553.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            1404..1559
                     /locus_tag="MB022_001553"
     CDS             1404..1559
                     /locus_tag="MB022_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466554.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1556..1780)
                     /locus_tag="MB022_001554"
     CDS             complement(1556..1780)
                     /locus_tag="MB022_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBT4466555.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2047..2382
                     /locus_tag="MB022_001555"
     CDS             2047..2382
                     /locus_tag="MB022_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466556.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2615..3556
                     /locus_tag="MB022_001556"
     CDS             2615..3556
                     /locus_tag="MB022_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBT4466557.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            3558..4448
                     /locus_tag="MB022_001557"
     CDS             3558..4448
                     /locus_tag="MB022_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBT4466558.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4491..5291
                     /locus_tag="MB022_001558"
     CDS             4491..5291
                     /locus_tag="MB022_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBT4466559.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5306..6157
                     /locus_tag="MB022_001559"
     CDS             5306..6157
                     /locus_tag="MB022_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4466560.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6154..6471
                     /locus_tag="MB022_001560"
     CDS             6154..6471
                     /locus_tag="MB022_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466561.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            6464..6646
                     /locus_tag="MB022_001561"
     CDS             6464..6646
                     /locus_tag="MB022_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466562.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            6648..7118
                     /locus_tag="MB022_001562"
     CDS             6648..7118
                     /locus_tag="MB022_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466563.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            7115..7657
                     /locus_tag="MB022_001563"
     CDS             7115..7657
                     /locus_tag="MB022_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBT4466564.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            7654..8037
                     /locus_tag="MB022_001564"
     CDS             7654..8037
                     /locus_tag="MB022_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466565.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            8034..8264
                     /locus_tag="MB022_001565"
     CDS             8034..8264
                     /locus_tag="MB022_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466566.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            8271..8474
                     /locus_tag="MB022_001566"
     CDS             8271..8474
                     /locus_tag="MB022_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBT4466567.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8471..8767
                     /locus_tag="MB022_001567"
     CDS             8471..8767
                     /locus_tag="MB022_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466568.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            8844..9257
                     /locus_tag="MB022_001568"
     CDS             8844..9257
                     /locus_tag="MB022_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBT4466569.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            9270..9656
                     /locus_tag="MB022_001569"
     CDS             9270..9656
                     /locus_tag="MB022_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466570.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            10148..10220
                     /locus_tag="MB022_001570"
     tRNA            10148..10220
                     /locus_tag="MB022_001570"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10181..10183,aa:Lys,seq:ttt)
     gene            10606..10866
                     /locus_tag="MB022_001571"
     CDS             10606..10866
                     /locus_tag="MB022_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466571.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            10909..11082
                     /locus_tag="MB022_001572"
     CDS             10909..11082
                     /locus_tag="MB022_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466572.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            11471..12304
                     /locus_tag="MB022_001573"
     CDS             11471..12304
                     /locus_tag="MB022_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBT4466573.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12297..13712
                     /locus_tag="MB022_001574"
     CDS             12297..13712
                     /locus_tag="MB022_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBT4466574.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13724..15253
                     /locus_tag="MB022_001575"
     CDS             13724..15253
                     /locus_tag="MB022_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4466575.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            15171..16223
                     /locus_tag="MB022_001576"
     CDS             15171..16223
                     /locus_tag="MB022_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBT4466576.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16225..16542
                     /locus_tag="MB022_001577"
     CDS             16225..16542
                     /locus_tag="MB022_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466577.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16669..17292
                     /locus_tag="MB022_001578"
     CDS             16669..17292
                     /locus_tag="MB022_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBT4466578.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            17307..18197
                     /locus_tag="MB022_001579"
     CDS             17307..18197
                     /locus_tag="MB022_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBT4466579.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18220..18435
                     /locus_tag="MB022_001580"
     CDS             18220..18435
                     /locus_tag="MB022_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466580.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18447..18788
                     /locus_tag="MB022_001581"
     CDS             18447..18788
                     /locus_tag="MB022_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466581.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
BASE COUNT         6828 a         2825 c         4062 g         5151 t
ORIGIN      
        1 cttagctatc tcatttaagg tttttaattt tggtgtatat tttccttttt cccattcgct
       61 tattgaggat gaactttttc ttcctagttt ttcggctagt tcttgttgat caatattata
      121 cttttctcta agaaacttta gattttcagc gaacatagta acacctcctt gagttaattg
      181 taacaccgtt tcggaaaaaa tgtaacaggt ttcttagaaa atattttagt gtttttaaag
      241 aagtatttac gcaaaataga aatcgatttc aggaaaactg aaatatgcac ttgacttcgg
      301 aaaaaccgaa gtatataatt tagtcagaaa gttaaagaga ggaggcgaat aaaatatgga
      361 taccgcgaag aaagaaaaat atacattgag acaattaaga gctttaaagg gttattcaaa
      421 agaagaactg tcaaggaaaa gcaaagtgac atcaagaaca atatttattt acgaaaacga
      481 tgtagataaa atgaggaacg gaaagtacgc cactctagaa aaaattgcta aggcattggg
      541 tgttagagtt tccgatattt ttttagaccc tgattcggaa aaaccgaaat caataacagg
      601 tgaaacaaaa caagtcagct aggaggtgtg aatagtggaa gagaagaata taaaagtacc
      661 gactcttgaa gaatatacaa aaattgttgt cgaaacagac gagcagaacc cgaaaacaat
      721 cgcagttatt acggcgaatg atattgaatc atgcgaaggt tttcgagttc gaatgacccc
      781 tagatatgat taatgttctt tatcacgtgg aggtatagga tcgtttccaa aactatcttt
      841 tgattgtatt tgtccattct ttttttggac aataaactct gattgctgtt ttttagaaat
      901 ctttttacca tactcaacag catctgattt attatcaaat agttttgtat cttcggaatt
      961 accttcacca cgtacagccc atttgccatt tctaggagat acccattgat ttttacccat
     1021 aacaattcac ctcgctttca agttaattct attaagaggt gaaaagaaaa caaactgatg
     1081 gaggtgagat tgtgagtaac aagccaaaaa tcactttgaa tggcacacct aatcttcaaa
     1141 gattagcaga tgaaatggct agaatacgtt ctagacattg gggagttact gtggtggcaa
     1201 ccataattga acaaaaagaa aagcccacac agccggcaag caagtagtgg gcaccataga
     1261 acaataatat tatatcacac actcgccact tccgaaaaga ggagttgctg ctctcctttc
     1321 aagtttgcca aagtccacgt ttcttcattt tggcaactcc tcttcttgga ggtggtgacg
     1381 gcgagagacg aaggagggat gacatgaagg aagaatcaaa aatagggatt ttgaatgaga
     1441 acaattacac aagattaatt attgaaacag aagaagggga aaaagtagca gaagttacat
     1501 tgaccgatgc aactcctgct gacggttatc gtattcgatt aactcctaaa tatgattaac
     1561 cttttggtgg gtgtggatcg tttccgtgac tatcacggct ttgaattttt ccatctctgc
     1621 catgaataaa tagctcagat ccttgatttt tagaaatctc tctagcgatt tttactgctt
     1681 ctgattttgt attagtatgt gcagtcgctt tagaatttcc agcacctttg acattccaac
     1741 caccattgga attaggtaca acatgttgat tttttcccat agttttcacc tccatttcta
     1801 tagaaatatt ttatcaaata gaaatggaga aattcaataa acgaaaggag ggatcaaatg
     1861 acattacaag agcatcaaca cttgatgcga aaactaaatc aggaatacca cgaggaaatg
     1921 aaacctagat tgctagggga acaatggcgt gaacgccaga agaagtggtt agaaataaaa
     1981 aagtgactca gccgtccaaa gcaatgagtc acaaaccaaa aattatacct aaggagattt
     2041 tatcacatga ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga atgccaaaag
     2101 caaggtgttt ctattatttg tacagcccaa aaagaagggg aacttaaaag tctcgtttat
     2161 ggtgagacaa cagaaatttt actttgtctt gctatgcaag aagaacattt agatgaaaat
     2221 ctcccattgt cagcacatat tatgcgtaga attgctgtgg atgcttacga acaagcgaaa
     2281 aatgaagaag aaaatcaacc ttcaaatcat acttttgtta ttaataacaa ggaagattta
     2341 gctgatgtga tgactcgtat tctcaagggg gaatttcaat gatgaatgaa aaaacaattg
     2401 atctactcat tattttattt ttcacgttag tgattgtagc gaaaagttat gtaatctttg
     2461 gcttgttatt cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga aaagaggtta
     2521 aacatgaacg agttagcaaa tcttgacaac tatttaactg atcctgatta tgctaaacca
     2581 ccatatgaag cgccaattga tgaggaggat gaagatgagt aaatctacct tagaaatgag
     2641 ccatcaagaa tggctcgaag accgcaaaag aggtattggt ggctcagatg ttgcaaccgt
     2701 acttggatta aacaaataca aatctcctta tcaattgtgg cttgagaaaa cgggtcaaat
     2761 tgaattgaaa gatttagaaa gcgaaccagc ttattggggt aatgttttag aagaagtggt
     2821 tgctaaagag tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc aagtatttga
     2881 acatccatta catccatttt taagagcaaa tattgatcgg gaagtagtgg gagaaaatgc
     2941 cattctggaa tgtaaaacag ccaatcaatt tctcggaaaa gagtgggaag gtgaagaggt
     3001 accgcttagc tatctctgtc aagttcagca ttacatgaac gttttaaaca aagactattg
     3061 ttatatcgct gttttgatcg gtggacaaaa attcatttgg aagcggattg aacgagatca
     3121 agagctgatc gatacaatca ctgaacaatt agtagagttt tgggaaacga acgttcttgg
     3181 aggtatcgag cctgtaattg acggaagtgg agcgactgct gacttcttaa aagaaaaata
     3241 tgcagatgta gaagaaaatc aaacagctct accatcacgt tttgatgaac ttatcgagca
     3301 aaaaagagaa ctcaagcgga cgaaaaaaga aattgaatca tctattcgtc aagtagacaa
     3361 cgagatcatc agtgagctag gtaaacgtga agccagtatc ggtatcacac aaagaaacat
     3421 tatcagctgg aaacttgttc gtacaagacg gatgaacacg aagaaactag cagagaaata
     3481 tccagatgtc gcaaatgatg aagagattta taacgttact gaatcaagaa ggctaaccga
     3541 aaaggagatc aaataatatg gcaacaaatg aatcgttaaa aaatcaattg gcagaaaagc
     3601 cacagaaaca agttgcacca ggacagttag ggcttaaagc tctaatgaat acaccaacaa
     3661 tgagaaagaa atttgaagaa gtacttcatg acaatgctaa tgcttttatg tcgaatgtta
     3721 tgactcttgt atctaatgac agttatcttg cagatagtga accgatgtct atcatgagtg
     3781 gtgcgttaac tgctgcaaca ttaaatcttg ggctagataa gaatttaggt tatgcatatt
     3841 tagttccatt caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa gctcaattca
     3901 tgcttggcta taaaggatat atccaattag cccaacgatc aggtaaatac aaagcattaa
     3961 atgtgatcga agtttacgaa ggagaactaa aaagctggaa ccgactgaca gaagagtttg
     4021 agtttgatcc aaatggtaga acgtctgatg aagtcattgg atatgttggc tatttcgagt
     4081 tactgaatgg attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa gctcatcgga
     4141 ttgctaacaa taaagatcgc gataagacaa agttaagtgg tgtgtgggca tctgattaca
     4201 atgcaatggc acgaaaaact gttttgagaa atcttctttc taaatgggga atcttgtcca
     4261 tcgaaatgca agaagccacc acatcggatg agagagtcca aagggttcaa gaagacggca
     4321 gcattattgc tgaaacagaa gttgaagaag atattcctga aagaaaagaa gcagaggtta
     4381 tatctgaaga aaatgaagat gtacaaactg gattatttga tgcatctaat ccgccgttaa
     4441 acaaataatg agggagcttt ctcccttgct tttctagagg agaaatacga atgaacacag
     4501 gatatgtcaa attacatcga aaagtgatga attcattcgt ttggaccaat ccttatatgt
     4561 ataaattatg gagcttgtgt ttgatgaaag cgagtcatga gaatcgcaag atacttttta
     4621 atggaaaaga gatacaagtg aacagcggag aattcgtcac agggcgcgat gctatcacat
     4681 ctgagatgaa cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct gtatggagat
     4741 ggctaaaaca atttgaaaaa aacggaatgt tgaacatcaa atcaactacg aaatacagcg
     4801 ttatatcaat aaaaaactgg tctttgtacc aaagcagtga acaacaaatg aacatcaaac
     4861 gcacaacaag tgaacaacaa gtgcacacaa acaagaatga aaagaattat aagaatgaaa
     4921 agaataataa aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac accgactcag
     4981 tttactacat tctcgcggag gagttattca aacagatttg tcagaatcag gaaatcaaaa
     5041 agccgaatct gcaaagttgg gcagataaca ttcggaaaat gatcgagatc gacaagcgga
     5101 ccgaaaacca ggtacgggga atgattgaat ggagtcagca caatgtgttt tgggcatcga
     5161 atattttatc tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg caagcaaatc
     5221 gtgattataa aacaaaacaa actaaaacgc ttgaatacga gaaatttagc acagatgagt
     5281 tgcctatttg agaggaggcg taggcatgga aactgttggg gaaatcatgg aaaagctgat
     5341 acagaaagtg cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt tgtatggatg
     5401 gcgtacgaag aaccctgatg gctcagaacg ttgtaagcca acctgtatgc aatgtgggta
     5461 taaagcattg cgagttcagg aagacttaca aacagaacga atttacaacg agagcctgaa
     5521 agcaagagca atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag cgttgtttga
     5581 ttgcacattg cagaattatc aaattgtcga tcaagaaaca agacaagcgg ttgaagtaac
     5641 caaacgcttt gttaattcag tcttgttagg aaatccaagt caccttgttt taactggaaa
     5701 acaaggaaca ggtaaaagtc acctagcgat ggcagcggct tgggaagtct tgaagcgatc
     5761 aaactacgat aaaaaaatct tatttatcgg gttacaggaa atgctggatc aaatcaaatt
     5821 ttcgtacaac aatcctgaac tcagaaaaac gattgaggga tcgttgattg cagatatcaa
     5881 aacagcggat ttagtcatca ttgatgatat cggttcagaa ctaggaaaag atgcatcaga
     5941 tagtcgagcg tttggcataa acacgctaaa ttcgttcttg gatgcacgac agaacctagc
     6001 aacgattatc acaacgaact tgcttggtga agaactgaaa aaagcttacg gtacgagaac
     6061 aatatcaaga atgtttgtca actctgatgg atttacgatg gtattttctc aaacagcaga
     6121 caagcgcata aaaccagtca aaggagcgat cgcatgaatt tgactaaaca agaaaaaaga
     6181 atctgtcgtc agataatgtt gcaaattata ggtgatcgtg acatggacga attatctcaa
     6241 caagagagtc gaatggttcg gaagataagt aagctaatcg gtgcagatac gatggatcga
     6301 tccaagccat tacccaaact gaattttgaa tcatttacgt ttgagaaata tagccatctg
     6361 agagaggtag gctatagtgt aaatgctatt cgaaaagcac ttggggtagg aagtagtgta
     6421 tgggcaaaat ggcggaaaga aaatgtaaat gaggtaaatc aaaatgatta agctaatagg
     6481 caaagagacg ctcaaggttt actatgtagc ggaaacaaag gcagaagtca gccgttggct
     6541 attgaaaatg tttaataaga ctagagccaa tgcttcagta agtcagaaag caagtgtatc
     6601 agaaacaggc atgcctgaag caatgtggat ttcaaggagg aactgagatg gcaatgatac
     6661 cgaagtttag agcgtgggat agagaaaaag acagaatgat ttatcctagc acgacaggag
     6721 tttgttttga aatgaatgat gatggtataa gtgtgcttga tattgatgta gattatccca
     6781 atgactatgg ttttcccaaa atagcttcaa tcctcatgca atctaccact ctgaaggata
     6841 agaatggtgt ggagatattt gagggggata tagcgcttat cactcataaa tcaacgaact
     6901 acgccgatac ttattggcac agttacgtag aagtttttag agcaaaaaac ggagcctaca
     6961 gaattcgcgg tgaacacatc tacgaaacag aattatatag taatagaaaa caattgacgg
     7021 tagcggggaa tgtttataat tcaccagaac ttttgaagag agatcgattt atgactacta
     7081 ccgaaaatcc agagttattg aaggaggaac agcgatgaat aaacaggaat tgattgataa
     7141 atatactgca gaaatatcta gattaagacc ttattgccct aatagacatt taattagcga
     7201 tcaacttaaa tatggtcttt ataaagaaat cctagaagat ttaaaacaac tagacgaacc
     7261 gcagaaacca gcggtgccta agtttgtggc ggaatggata gaatacgcta aaaagaaagg
     7321 cgatagtcta gctatttcat tcaagccgtg gaacctctac ggtgttgagt atagcaagac
     7381 tgatagatgg attgaagata atcaaggaac gtttgctcgt gcttggctaa ttggctacga
     7441 ggtcgagaaa gagccgttgt attatgtgaa gttgccagtt gtgtatttta atcattggga
     7501 tttagagacg tatctaatga aagatgatag aggaaatata acaattgcag acaacaacga
     7561 ttttgatgat atgaaattta cggaatcaga aataaaagca attgatgaaa gatactggcc
     7621 atttgctgtg ccagtggaag aggtggcgga aggatgagca atcttaaagt tggagatatt
     7681 gttttttaca aaggtgacga aggcatcatc cgagaagtta tgacggatgg aaaagaaaat
     7741 ttttataggg tagatgtcag cagaaaatct atactctacc tgtatgaaga cgaattagat
     7801 ttaggtactc aaaaactcaa cgacaaccag aaaatggtgt ttgattatct taaatcaaac
     7861 gttgaacaag gtggcaaatc agttatgtat tcgatattcc ttctaggtga ttgggactct
     7921 cgaattggag cagcaagaaa cgttgatata gcttactgtg gattaaatgg taagcaggaa
     7981 gcagaagtcc tatcagcatt cgctcaatgg ggattagaac aggaggaagc ggaatgaaat
     8041 acgaaatacc actaagtgaa gcagacgtcc agtcaattat taacggtcgg gaggttaata
     8101 aaaaacttcc tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag
     8161 ctccagtatt aattgatcgt tttaacgtga ctgattctgt ggtagagaac catttaaaag
     8221 aatttcgatc aagtatagac gacactttca gattagggag ttgattgaca atgaacaaca
     8281 gacatcgcag aataacaaaa ctaagaaaac aggaactgaa tgtactaaag acaaagtttg
     8341 aaaaagaata tggaatttca gcagaagaaa catataaagt ggcaagtcag tgtgttgctg
     8401 atgcgagtaa tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg
     8461 aggaaatgag atgaaactaa aagacggatt ttacgctagt agtcacggca tcggcggttt
     8521 aatgctagat atgccgacaa agaatcctaa aacacgtaag aaaccaaaag tcaaagtcgg
     8581 tgacatggtt cgctgcgaag cagaagggtt catctatcca tttcgtggat atgtagaaca
     8641 cgtctataat cactcagcaa tcattcgtat tgaaaacacg atggaatgtg ataaatggac
     8701 agctaaaagc aaagagaatt tagcagtggt gagattggtg gatattgaac taatcaatga
     8761 caaataaaaa agccggatcg ctccgactga ttcaataaat tccacaagtt tattatatca
     8821 cataaaaggg gcggtttgac ttgatgcaat tgttacgaga ggtagctttc aaacagacaa
     8881 gatgtaatgc gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct
     8941 ctttgataga tattaagtcg ccgattatta cggacatgcc gaaggcaccg aagcacggca
     9001 ataaggcaga agacgcgatc attcagatga tggatataga agcagagaga gacgcgattt
     9061 tagcggcttt gatggcgctt agtctgatta gtcgtcagat actctactac agcttttgtg
     9121 tgccagatag cttctcaaac tacaggatta gccgtgaagt gggttattca gaaagaagca
     9181 tacaacggat gaagtcggaa gctctgatag agtttgcaga agcatataaa catgggaaaa
     9241 taattgctta taaataggag gactatataa tgtggaaaga ttatgtatca ttgaaagagt
     9301 tgaaaaaaga tcttattttc aaaaaaatcg tagaatggtc agagagtgaa ttaattttag
     9361 aagatggaac caaaatggaa gttgtatgta gcgaatcaga ttgctgcgca tgggccgaag
     9421 gtgaattcaa aaatgtaaag cttgatgcag tgattacaga tataaagatt tttgataaag
     9481 gtaaccgtct ctataatggc gatggacata gttcttatgc cgaagtcgtt gtttatcata
     9541 atcgaaatga gattgctaaa gcggaatgta cagcaaacga tgggaatgga ggctactatt
     9601 acagtgtctg tgctttaaaa gtcaaagaaa aactttgtgt agtgactgat gcataaaaaa
     9661 tggcggtttt ttggcggaat gatggcggtt tttagctatt taccagtgat attatggtag
     9721 tgtcgaaaga ttagtgatag gtctgagaca aaataaaatg taagggagga aatctccctc
     9781 atcgtttaat taagcttcga tagacagcag cggaaatatt aagaataagg atgtgaattt
     9841 taactccttc taaattgttc ttattatcta tcatccgttg ctgtctattg ttattatgtc
     9901 actgtggcgg aaagggtaga cgcttaaaaa taaggtcaat acgtcgaggg atagccttaa
     9961 cgttttatga tttgaccatg caaggttcga ttccttgcca gcgacttaga cgaaaggtag
    10021 cttaaatatt aaccacatca acatcgtcag cgagttgata aaggtaacta gcagactcta
    10081 ggatttaagc cagtcttacc gattagtctg ttatagtagc aagcttgcta gaggtagctc
    10141 ctctggttga cgtgcagctc agttggtaga gcgcctgact tttaatcagg aggtcgccgg
    10201 ttcgaatccg gtcacgttaa taaagaacct acggaaacaa tcaatcttat cggatgccga
    10261 ttggttggct gaattaagca gtgtgtctgt gaatcagtta ataggcataa aatactagca
    10321 cagacgtgtg ccactctcag gtgtaggtta ggagagaaac attagcaacc gagggttgga
    10381 aatgggcgct caaagtacac gagcaaggcg aggtcgatag taatcgatgg aatcggtgta
    10441 ggttgctatt acataattga ttgggttaga ttgagttttg ggattcggta caaatgaatc
    10501 gtcaaatgac tcaagcacag gatcggaaac gtccctgcct gtgcattaca tattagatca
    10561 ctcgttgagt ggtcttttta ttttagaaaa gagaggattt tgaaaatgaa tcatgagaag
    10621 tttatcgaaa aatgcaaggc tattgtacgt gaaagaattg agaatgagat tgctgaccta
    10681 agtggagcag tacctgagtt tagtgttttc atagtctggt catgcaaaac actacagaat
    10741 agcaaagcat tagtcagcgc tagcttaaaa ggagcaccgt attttgaaat tacgttgaac
    10801 ggggacaaag gtgaaatcta tgtagatact tatctcaaaa aatcaaatga atgtatcaaa
    10861 gtctagcagg tgctaggctt tttctttaca taaaggagga atgatctcat gaaacaatat
    10921 accgctaaag atttcgagga aatgaagcga ttaaagaagg actatgaaga agttgatatg
    10981 gagctaactg ttggagtcat tcaacgaaga ctgcgggtcg gattagagac agcaaaggct
    11041 atttacaatg atctaaatgc tattgaagag aagaatggct aatgaggaac tactggtata
    11101 tatcgctaac taatgaatat cctcgaacca ttgatgattg ttcagtgcgt gttgtgcgtt
    11161 ctgtacaaat caaagggaag tactctattg tcgaaatggg gagagaagca gaaccatgtg
    11221 agattgatgc gtgcaagctc gtttattgcg gtcatggttt ctatgatgaa ccaaacattc
    11281 aaaataatat taacaagaat ttgagggatt agaatgcaag aaatggctta aaaatctcca
    11341 atcaggacaa atcaagttag acaaagtttc cgatttaaag atattaattg aagcagatct
    11401 aatgttgaaa gatattgaaa attagaaaac aaaactcaac ctaagaagat tgcgaggtgg
    11461 tgtgtattga atggcaagac aacgtgatcc aagacgtgat gaagccaaaa gaatttggtt
    11521 agaatccaac ggagaaaagc agttaaagga aattgcatct gaattaaatg tttcagattc
    11581 tcaggttaga aaatggaaat cgcaagacaa atggagcgct gaattgaaaa gtaacgttac
    11641 caatggcaaa agtaacgtta ctaatcaagg tggcgctcct attggtaatc aaaatgctaa
    11701 aggtaacaaa ggaaatagcc gagcttctcc gccagtgggt aataaaaacg ctttgaaaac
    11761 aggcgagtat gaaaccatat tttttgagac actaagtgat gaagagaagg acatctattc
    11821 tagtctgaat gataatcctt cttttgtttt gtctgaagaa atacgtctac ttaagataag
    11881 acaatttcgt atgatgaaga gaatccaaca agctgaagct ggactaaatg atgaagaagt
    11941 cgaacgattg cagcagctaa gaaagattaa aaatccgatt gaaaaaaatg gtaaaaagct
    12001 agaaatcaag cgtgaggtta tgcaagatgt gcagattagc agaaaaaaac atcgcaaaat
    12061 tgatgatatt ctttcaattg aagattcatt gactcggatt agcaaccagt tagctaaagc
    12121 catcaagcaa atgaatgaac tttatatgaa tgaatacaga actgatttaa ttaaagctca
    12181 gactgataag atccaagctg agacaaatga aattggcgga aataattcag gtgaagaaat
    12241 agaagaatgg aaacaggcag ttttaaatgc cgcaaacaaa cgggcggtga aagaaaatga
    12301 ataatgaatt tattcctttt gctgatattg gttctgccat tgattattac tatgataaac
    12361 cagtagcttt ttgccaagat attttgcatt tgaatcctga tgaatggcaa gaaaatgttt
    12421 taaatgattt agctgaattt tcaaaggttt ctgttcgttc tggtcaagga gttggaaaaa
    12481 cagcattaga agcaggagca atactttggt tcttaacgtg tcgaccctac gctaaagtaa
    12541 tagcaacagc tccgacaatg aagcaacttt acgatgtact ttgggcagag gtagctaaat
    12601 ggttaaatga tagcttgatc aaaaacttac tgaagtggac aaagaccaaa atttatatgg
    12661 ttggtgattc agagcgttgg tttgctacgg ctagaacagc gactaaacca gaaaatatgc
    12721 aaggttttca cgaggaccat atgttgattg tggtagatga agcttctggt gtgtctgatc
    12781 caattatgga agctattctt ggtacgctat caggttttga taataagctg ttgatgtgtg
    12841 gaaaccccaa taatattgaa ggtgtttttt acgattccca caattcagac cgtgataaat
    12901 acagagttca taaagtatca agctatgata gtaaacgtac aaacaaagac aatatagaaa
    12961 tgattcttaa aaaatatgga aaagaaagtg atgttgctcg tgtccgtatt tttggagaat
    13021 ttcccaaagg tgcgttggat tcatttatca gtcttgaaac ggttgaattg gctacagaaa
    13081 aacaaattag tgattcttta gtcaataaaa caacggttgc tcatattggt gttgacgtag
    13141 ctcgatatgg tgatgattct acgattctct ttcctagaat tgctaccagg gcattggagt
    13201 atgagaagta ttcaaaacgt agcaccatgg aaacaacagg atatgtcatc aacatggcca
    13261 agaatctaat gagtcaatat ccgagtattg ataaagtgat gattaaagtc gatgacactg
    13321 gtgtcggagg tggtgtaacc gaccgcctag aagaacttat agaagacaaa cattatcctt
    13381 ttgaggtgtt tggagtgaat aacggttcaa catcagaaga cgatttttac gataatttag
    13441 gtactcaact atggggaaac atcaaggaaa tgttagaaga aaatatgaca gcaaatctta
    13501 acggagaaca gcctgttatt gaattgcctt ctgatagttc gttaatcaaa gaattaagta
    13561 ctcgcaaatt caaaatgaca agtagaagtc gtatacgttt agaaagtaaa gatgatatga
    13621 aaaagcgaaa tattggtagt cccgatattg ctgacgcact ggctttagcg ttttatgagc
    13681 caccaagtca ctatcaattt attcaatttt aggaggtgag cttttgatta gtacagtatt
    13741 atctttagag agatataaga agctacgtgt taaatatgca acacaaattg aagatggaat
    13801 gtttgatccg aatggtttta tagaagatat gaaaccattt tttgctgatc gcgaaagaaa
    13861 atatctagct tatactagcg aaaagaatga aatagataat agaccaaagc ctaacactga
    13921 tattgtaaaa gttaataata aacttcatgc tggtatgtat tcaattgtcg tagaccaagc
    13981 agtcaatcat tttactggta tacctatcaa atgggattac gatgtatcgg aacaaaaaag
    14041 aacactcatt caaagattaa aagataaatt cttaaaaaat gatatagaac ttccgacagt
    14101 tccagaagct tttaacaaat taacaagcaa tcttgattcc atgagattcg caatgcttga
    14161 ttctgaaact gcaacttttc aaggagcttg tggagtagct ttccgattgt tagagcctgt
    14221 ggaagaagat gatggttgga agttaagagc aagtaatatt gaaccttgga gagcagaaag
    14281 atatggaaat gccggaatct atatcaaaga aaagtatgac tcttaccaga aaaaattttt
    14341 tcaagaaatg aaagttatga caagaaataa aattctgaca tatgcttgct atggtgattt
    14401 gaattttgtt acaagcggaa cgttcaaaaa aattgatgag accgataacc ctttggggac
    14461 gattaccttg gcagaattta aaaataatac gaatcgttat tgtgattttg aagtagctga
    14521 ggaaattgga gatgcaattg atcgtgcttt gtctgatcaa caaaacgaaa tcgaacaatt
    14581 taagcttgcg tacatgcttg ttactggcac aacaatgagc aaaggcactg ctaaagaaat
    14641 gatgaatcag ctagggatta tcaacttaaa agaccctact gcaaaagctg aatatgtcac
    14701 aaaaaattta gcaaaagatt tcaatgaata tcatatggat ctattgaaaa aacagtttta
    14761 caccatttgt aaagcaatcg attttaacga tgaggtgttt aaatcaaaca gttctggaga
    14821 agctcgcaag tggcaaatca ttagcctaga agcgaaaaca aatactaaag agcaatattt
    14881 tagagaagga ttgaaagaat gcgcagaaac aattgctgct tttcttaagt ttcatgacaa
    14941 agtagaaatt gagcctgaaa aaattatttt tactttctct agatctttgc caactgattt
    15001 aagttattta gctgaagctt tacctaaact tgcaccatac gtatccaaac gaactattca
    15061 gagccaaatt ccatttgtaa cagatgttga ttatgaaaat gaaatgatgg aattggaaag
    15121 tggaagtgct tatccagata gtgaatacaa ttttggcgga ggtggcaata gtgacgatag
    15181 aaacgaagta ttggaccaaa cgtcgagaac tggaggatca agcaaggctc aaacaagaaa
    15241 atcagacact taaaaaatta actagtgtat ttcctgaagc actgaaagag atacaagcaa
    15301 aactattatc acaagctgac ttacacaata tcacttatcc agaaatgatg gagttttata
    15361 gtacaagtaa tcagaaaaaa tatcgtgaat atgtggaaaa aaattataag tcattaaaaa
    15421 tgtatgatgc aaaatacaaa gagtttatcg atgaattttt tccaccattt gactatgcaa
    15481 aagtcaatcg cttattacaa atacgatcag atgtatttaa aattcttgca gaatatgcga
    15541 tggatgcaga tgtgaatcaa tatttttctg atcgcttaga ggaaattctt caaagaacat
    15601 attcttctaa tgctaatgtt tttgttcaac ttttaaacgt tgatatacca aattatttac
    15661 cagaaaatga aattaaacat tatttgaact atccatggtc aggaaagaca ttttcaagaa
    15721 gactttgggg aaatatttca tcgcttgaac agaaactctc taatgctatt gtaaaaagtg
    15781 ttgctagtgg agaaggcgtt atacacgcat taaacaccat gagattagac tcagaaattt
    15841 gtgacatgtt taagttagaa gaatcaaagt ataacaaagc gatagaaaat ctcgttcgaa
    15901 cggagtatgc aaaatttgca caagatggta ttgaaaaatc atatttagaa acaggtattg
    15961 aggaatacaa cgtattgact gcaaaagatg agagagtttg ccggatttgt ggaggaaagg
    16021 caagtaagaa tccctataaa ctgaaagatg ctgtcatagg tgaaaatcga gcacctttcc
    16081 atagtcgttg tagatgtacg gatgttccta atttaccaaa attaggaaag gatattgatg
    16141 aagaatatga ccgtttattt ggcgatttat tagatgagtt tgcacatgat tcttttggaa
    16201 ttaatttgaa acggaggaag tagaatgaaa gatttttttg aagcagtact aacaattaat
    16261 gtaaatgctg atattgcaga agcctacaaa acagctattg agtctgagaa ccatcctaat
    16321 ggcttgagag accattggaa tggcaattat gcctacgtgg ttattggcga tcaaactgtt
    16381 aattatcaag ataatactcc agttgataag aataccgtta atttaacgat tcaattatta
    16441 tctcattcat taccaaattt aaaagaaaca gttgattggt atgaaaagat gggatgtatt
    16501 gttgttagaa ctgactacaa agaaggaaag tctagtaatt aggctttttt attttgtccg
    16561 aaatgacgtt aaactagcgc aatactgggc ttggttgaat ggtggggcgc aactattaaa
    16621 actcaaagca atgcggggcg tgaaaacgaa tcgtggggcg aaaggagaat gagcatgaaa
    16681 cacaaatcat taatgccaat gaatttgcaa tactttgccg aaggtgacga tcaaaagttt
    16741 tcttttgatg acttcaaatc ttttgtggaa tccaatgaag aagctcaaaa atttattcag
    16801 tcacaatctc aaagtgttgc cgacaaacaa ttggaagctt ggaaacaaaa taacttagat
    16861 aaaattaaac aggataccat caaggaatat gaggaatcta agaaaaacaa gtcacctgag
    16921 caaattcaac tggaaaaact acaagctgaa tttgaagcag aaaaagcgtt gcgtgtgaca
    16981 agtgataata aagcatttgt tgcagaacaa attgctggat tagaactaga tggagagtta
    17041 aaagagtcta tttctcaatt tatgctaaat aatcttgtta gttcggatac agatttcact
    17101 aagaatgctg ttgaaggttt tacaagtgtc ttgaatgcaa ttaatgagaa acatgcagat
    17161 gcattaaaag aactacaaat gaagtctgca tttggtggaa ctcaacaatc gaataaccag
    17221 gttcagcaga acaatgaaac attaacaaat ccagaagaac aattaggaca aattcttcaa
    17281 caatttaact aggagagtga aaaattatga aaaaaacatc tttaaataat ttagagtatt
    17341 tggatatttc accagcgatt aatgctatgc aagtaccaaa tacacctttt ttaagctatt
    17401 tatttggtgc tggaaaaaca gagccagcaa actcgacaga aattaaatgg cgtgaatatg
    17461 atatcaacaa cgatgattct tctgaaaaac ttgagggcgg agaatatcca gatgctgaat
    17521 caggtcgaac ttggtttaac aactatactg aaatttttag aaaatcaacc tctgtatctg
    17581 gtacattaga tgctattaat gtgaatggag tcggaaatga attaactaat caagtagcac
    17641 tacgtggtat ggaaatgaaa attgatttga accgaaaatt gattactggt gtaaaagctg
    17701 atgaaaatgg ttctaaaggt cgtcgaatga atgggatttt gaacttgatc aattcagcaa
    17761 ataaggcaga aacagccact gcgggtgcag taacaagaaa agatatcgat actttattta
    17821 aattgatgta tgaaaaaggt tatatgggag aaaaactatg cttgatttct ccagatatgc
    17881 aggagttaat gactgatgag ttagatggaa aatcaacaaa aattgttcag ttcggggaaa
    17941 gagtaacttt tggattgcaa attggaaata tcgtgtctaa ttacggtaca ggtattgctc
    18001 tactagaacc atcattgcca aaaggaacaa ttgccgcaat cgatactaat tatgtgaaac
    18061 tacgtccatt acgtgaatgg agagcagaag aacttgcaaa aacaacggat tctagacgta
    18121 tcggtcttgt aggtgaatac tctcttgaat acaatgcttc aaactctggg gcaattttaa
    18181 atttaaagtc tgaataaaag ggagttagta ctcccttttt tgataggagg aattacagtg
    18241 gtaaaaaaag atgaaactaa aaaagatgaa gtcgtgaaat atagagtagg taaaactaaa
    18301 aattttgttg ggtttgttca tcctaaaact cgtagattta tcacagcaga ttcaaataac
    18361 gaatttatca tttctataga tgataaagaa gcaattgcaa ttttggaaga tgcaattgat
    18421 gttaatgaaa tttaggaagt gatctgatgg atgaatcgct aaaaacggaa atcattgagt
    18481 ctacaaaaga agattttcca gatttgagtg aagaacgcat aactaattta ttagaaataa
    18541 ttttgctaga aattgaatca tacaacactt gtaaaaatga tatttcatgg gaaaagttaa
    18601 aaagcgtgat tagtgaagtg ttgtataaaa taataaaaaa tgaatcagaa aaaacagtat
    18661 cttcagttag acgtggcgat acaacgatta gttatgcttc aacaacaaat gatgttagtg
    18721 agttacttct aggctatggt gatttgatac gaagagttat tggttgtgga ggattggagt
    18781 ttttttaatg aatgaagcag atattttaga aactacttat gaagatagtt gtattattga
    18841 aagactgacg gacattgaag attcta
//
LOCUS       DAGCXG010000044        18633 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGCXG010000044 DAGCXG010000000
VERSION     DAGCXG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.045"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            122..1450
                     /locus_tag="MB022_001582"
     CDS             122..1450
                     /locus_tag="MB022_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBT4466582.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            1652..2485
                     /locus_tag="MB022_001583"
     CDS             1652..2485
                     /locus_tag="MB022_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBT4466583.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2501..3238
                     /locus_tag="MB022_001584"
     CDS             2501..3238
                     /locus_tag="MB022_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBT4466584.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3393..4361)
                     /locus_tag="MB022_001585"
     CDS             complement(3393..4361)
                     /locus_tag="MB022_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBT4466585.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            4593..5426
                     /locus_tag="MB022_001586"
     CDS             4593..5426
                     /locus_tag="MB022_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBT4466586.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5460..6299
                     /locus_tag="MB022_001587"
     CDS             5460..6299
                     /locus_tag="MB022_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4466587.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6325..6885
                     /locus_tag="MB022_001588"
     CDS             6325..6885
                     /locus_tag="MB022_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4466588.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7084..8805
                     /locus_tag="MB022_001589"
     CDS             7084..8805
                     /locus_tag="MB022_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBT4466589.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            8970..10112
                     /locus_tag="MB022_001590"
     CDS             8970..10112
                     /locus_tag="MB022_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4466590.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10128..11339
                     /gene="thiI"
                     /locus_tag="MB022_001591"
     CDS             10128..11339
                     /gene="thiI"
                     /locus_tag="MB022_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBT4466591.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            11912..12559
                     /locus_tag="MB022_001592"
     CDS             11912..12559
                     /locus_tag="MB022_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4466592.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    12688..12904
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12952..15597
                     /locus_tag="MB022_001593"
     CDS             12952..15597
                     /locus_tag="MB022_001593"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBT4466593.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            15907..17220
                     /locus_tag="MB022_001594"
     CDS             15907..17220
                     /locus_tag="MB022_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBT4466594.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17210..17863
                     /locus_tag="MB022_001595"
     CDS             17210..17863
                     /locus_tag="MB022_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4466595.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            17918..18589
                     /gene="radC"
                     /locus_tag="MB022_001596"
     CDS             17918..18589
                     /gene="radC"
                     /locus_tag="MB022_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBT4466596.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT         6283 a         3129 c         3961 g         5260 t
ORIGIN      
        1 ttcttcatga agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt
       61 gtatttctaa attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg
      121 gatgaaagct attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat
      181 caaacagcag gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa
      241 tgtttcagca catgtggaga atacaccggt attttctata gatttggcta cggggaaagt
      301 ggctaatcaa atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca
      361 taagatgctg aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt
      421 ctgggataaa tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga
      481 ggcaatcgat agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca
      541 atgggatatg atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc
      601 ggaaagtgcg aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg
      661 taaagatgca gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga
      721 agaaaagaaa gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac
      781 acctccaaaa gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg
      841 aggactgaca ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt
      901 tagcgtgatc aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat
      961 gctaggaaat gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt
     1021 caaaaaatta gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt
     1081 aggtcaatct tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga
     1141 tctatttgat acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct
     1201 gatgacccat gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg
     1261 gaaagtcgaa aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga
     1321 tgcctggatg gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga
     1381 gcaattaaat gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc
     1441 tttagcatag acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta
     1501 agccagctct ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat
     1561 tctaaataaa ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag
     1621 tagaataaaa ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct
     1681 gaaattaaat ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat
     1741 aaaaaccgaa catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt
     1801 tgaagcagaa gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta
     1861 tatgctaggt gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa
     1921 aggggatcaa cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca
     1981 atttggtttt cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga
     2041 agattggtgg acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga
     2101 aacgaatcga tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt
     2161 gaaatcggtt gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg
     2221 taatgtgttc tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg
     2281 gaatcgggaa caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt
     2341 agatgcttat caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta
     2401 ccagttatat tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt
     2461 agatcaatta ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa
     2521 aaagcatgaa gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat
     2581 gttaggagta gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga
     2641 attcgtcaat agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg
     2701 tctccctaga gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt
     2761 tttctgttat cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga
     2821 atctgttttc gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat
     2881 cattgctcct tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa
     2941 gattgattcg ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca
     3001 gcatgttaat aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt
     3061 gactacgcat gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa
     3121 tgagatcgaa agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga
     3181 aatccgaatg caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa
     3241 aagaggttgt gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt
     3301 tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc
     3361 tctttttaag tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct
     3421 tcacccgaca aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta
     3481 atactgttac agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca
     3541 taaacaggct ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg
     3601 acaagaagct tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct
     3661 tcgattacca aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc
     3721 ccagcatatg ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt
     3781 gtttctaaaa gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc
     3841 aatgtaggtg ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca
     3901 gaatggatct catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt
     3961 gcgacacgaa tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa
     4021 cgcattgccc cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa
     4081 gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca
     4141 taagcggaca agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag
     4201 ggaagattga agatatcttc aactaataat tctacatgct tggggattgt gatttctgca
     4261 tctaataatg ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg
     4321 gcgatcgttc cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga
     4381 ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt
     4441 gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga
     4501 ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta caagaaagca
     4561 gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta
     4621 aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc
     4681 tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga
     4741 attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca
     4801 gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt
     4861 tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc
     4921 cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt
     4981 ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc
     5041 ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc
     5101 cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc
     5161 tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta
     5221 aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca
     5281 aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg
     5341 atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca
     5401 atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta
     5461 tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt
     5521 tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag
     5581 caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt
     5641 atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt
     5701 ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg
     5761 tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt
     5821 ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa
     5881 tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa
     5941 aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac
     6001 atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa
     6061 aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg
     6121 aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa
     6181 ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc
     6241 aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt
     6301 caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca
     6361 ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa
     6421 gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca
     6481 cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca
     6541 gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat
     6601 gcactgacag atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct
     6661 gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa
     6721 cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat
     6781 gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag
     6841 aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat
     6901 ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg
     6961 aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat
     7021 ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca
     7081 taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc
     7141 gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa
     7201 aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa
     7261 atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac
     7321 atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat
     7381 gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac
     7441 caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa
     7501 gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa
     7561 gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa
     7621 gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa
     7681 gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa
     7741 aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc
     7801 agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct
     7861 gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca
     7921 gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat
     7981 gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt
     8041 gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac
     8101 acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc
     8161 gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa
     8221 attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc
     8281 gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa
     8341 gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa
     8401 aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac
     8461 cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat
     8521 cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac acatgattta
     8581 gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca
     8641 cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac
     8701 tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc
     8761 aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt
     8821 aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa
     8881 attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag
     8941 aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc
     9001 caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc
     9061 catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc
     9121 agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag
     9181 aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata
     9241 tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat
     9301 taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat
     9361 tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag
     9421 taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc
     9481 atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag
     9541 aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt
     9601 tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa
     9661 gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac
     9721 gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc
     9781 taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg
     9841 caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct
     9901 tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg
     9961 caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa
    10021 tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc
    10081 agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg
    10141 aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca
    10201 tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg
    10261 ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa
    10321 aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg
    10381 tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa
    10441 catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga
    10501 atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc
    10561 aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa
    10621 cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt
    10681 ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg
    10741 aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag
    10801 atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta
    10861 ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc
    10921 gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt
    10981 tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca
    11041 atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca
    11101 ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt gaagattgct
    11161 gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat
    11221 atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg
    11281 aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag
    11341 aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc
    11401 atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa
    11461 gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat
    11521 caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg
    11581 acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt
    11641 tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt
    11701 gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
    11761 tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc
    11821 tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa
    11881 atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa
    11941 agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg
    12001 tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat
    12061 ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc
    12121 ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt
    12181 agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa
    12241 tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg
    12301 agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt
    12361 agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa ttgtcaaagg
    12421 aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa
    12481 tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta
    12541 ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg
    12601 taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga
    12661 ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac
    12721 attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta
    12781 gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact
    12841 tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct
    12901 agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag catgacggaa
    12961 gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat
    13021 tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc
    13081 gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc
    13141 ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct
    13201 ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa
    13261 gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa
    13321 gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac
    13381 agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt
    13441 accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa
    13501 gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat
    13561 catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca
    13621 gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg
    13681 ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat
    13741 gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca
    13801 aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat
    13861 ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa
    13921 gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac
    13981 agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg
    14041 tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca
    14101 gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat
    14161 gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa
    14221 gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa
    14281 gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc
    14341 tggccagata cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc
    14401 ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact
    14461 ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg
    14521 aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga
    14581 gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc
    14641 agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt
    14701 ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa
    14761 tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag
    14821 ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat
    14881 gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt
    14941 tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg
    15001 catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat
    15061 tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct
    15121 aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg
    15181 aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat
    15241 caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg
    15301 atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa
    15361 atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa
    15421 gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt
    15481 tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat
    15541 ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa
    15601 tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt
    15661 tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat
    15721 tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca
    15781 tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt
    15841 tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa
    15901 ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca
    15961 cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa
    16021 gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga
    16081 agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg
    16141 ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt
    16201 gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt
    16261 actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac
    16321 gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca
    16381 atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt
    16441 gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga
    16501 aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg
    16561 ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga
    16621 gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta
    16681 gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa
    16741 ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg
    16801 ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat
    16861 acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc
    16921 aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag
    16981 caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta
    17041 agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga
    17101 ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt
    17161 tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa
    17221 tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc
    17281 cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt
    17341 cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg
    17401 caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc
    17461 gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca
    17521 tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa
    17581 gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc
    17641 aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga
    17701 aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc
    17761 ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg
    17821 ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg
    17881 tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga
    17941 agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc
    18001 ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc
    18061 gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt
    18121 gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt
    18181 gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac
    18241 cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt
    18301 ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg
    18361 ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg
    18421 tgcagcaagg atcgttttag cgcataatca tccttccgga atatataagt tagtacacat
    18481 aaattatata aggaaattta ctgttatatt gccatctgga aatactatta tgttatcgat
    18541 aattgaacgc cataatgcat ttttttcttc ttccgaagat tctttataaa tttcatcgaa
    18601 ttcttttgat agaataaatt cgacattttt tat
//
LOCUS       DAGCXG010000045        17819 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGCXG010000045 DAGCXG010000000
VERSION     DAGCXG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.046"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="MB022_001597"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="MB022_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBT4466597.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="MB022_001598"
     CDS             766..2139
                     /locus_tag="MB022_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4466598.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="MB022_001599"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="MB022_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBT4466599.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="MB022_001600"
     CDS             3194..3634
                     /locus_tag="MB022_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBT4466600.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="MB022_001601"
     CDS             3778..4479
                     /locus_tag="MB022_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBT4466601.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="MB022_001602"
     CDS             4476..5597
                     /locus_tag="MB022_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBT4466602.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="MB022_001603"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="MB022_001603"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBT4466603.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="MB022_001604"
     CDS             6976..7884
                     /locus_tag="MB022_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBT4466604.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="MB022_001605"
     CDS             7919..8191
                     /locus_tag="MB022_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBT4466605.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8202..9248
                     /locus_tag="MB022_001606"
     CDS             8202..9248
                     /locus_tag="MB022_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBT4466606.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="MB022_001607"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="MB022_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBT4466607.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12945)
                     /locus_tag="MB022_001608"
     CDS             complement(12259..12945)
                     /locus_tag="MB022_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466608.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(12923..13417)
                     /locus_tag="MB022_001609"
     CDS             complement(12923..13417)
                     /locus_tag="MB022_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4466609.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="MB022_001610"
     CDS             complement(13437..14657)
                     /locus_tag="MB022_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466610.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="MB022_001611"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="MB022_001611"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBT4466611.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(16733..>17819)
                     /locus_tag="MB022_001612"
     CDS             complement(16733..>17819)
                     /locus_tag="MB022_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4466612.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         5873 a         3221 c         3734 g         4991 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggacga
    16621 ggacgaaccg aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt
    16681 tacatggatt tgccaccggt ttgccaccgg tggttttttt cggtggcaaa aatcaagaat
    16741 tctttctaag attttccatg atatcgaccg tttcatttct catcttatct gttacatgcg
    16801 agtaagtatc catcgtaatt gaaattctgc tgtggcccaa tcgttcagag atttctttca
    16861 tttttgcgcc attttcgaga agaagtgtag catgagtatg tctgagagaa tggaagttaa
    16921 aagagaggga gagtgcattc gatatcctcc tagtattcca tttcactaca cttggcgtaa
    16981 ctagctcacc gtcttccttc gtacatactg catttgaatc aatgtagagc tttccatact
    17041 tcattcgatt ttctaattgt tgtttcttat gttttttcag aattgctagc aaggtttgtc
    17101 caataaaaat cgttcgattg gaactgcttg tctttggcgt accatatacc catgcgccgt
    17161 cattctttac catttgtttc tctacagtaa ttgttccatt tgaaaaatcg acatcatccc
    17221 acgtcagacc acaaacttcg ccaacgcgca ttcccgtata aaatccaata ttcaaaggaa
    17281 tatagaaagg atgtccttca ggagtaattt ctagcatatg atcaaagtcc tcaagagaaa
    17341 tgatttttag atctttttta gtcgttggtc gtgcttcata ttttggtatt tttacataca
    17401 gcataggatt ttgcttgatt aatccccaag gataaaccgc catattcagc gcattcttaa
    17461 ggacagagtg agtaatagtc attgttttct tcgagtaacc ctttttaaat tcagcattga
    17521 tgaaattttg taaaagagca ggggagagat ccgtaagttt tttctttcct aaataaccgt
    17581 ttatatgatt tttgatggta aatcggtagt tttcataggt attgtatttt agatttagtt
    17641 taacgtattc ctccatccaa aaatcaaggt attgttttac tcgagtatcc gtacctaaaa
    17701 agtattgtcc tgtttcgtca atatctgata aaacttttcg taaagcagct tcggcctctg
    17761 gtcgggtgtc tccgccaact ttctccactt tttttcttga gccatcatca ttgatatct
//
LOCUS       DAGCXG010000046        17344 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGCXG010000046 DAGCXG010000000
VERSION     DAGCXG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.047"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(27..725)
                     /locus_tag="MB022_001613"
     CDS             complement(27..725)
                     /locus_tag="MB022_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBT4466613.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="MB022_001614"
     CDS             complement(718..2070)
                     /locus_tag="MB022_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBT4466614.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="MB022_001615"
     CDS             complement(2293..3693)
                     /locus_tag="MB022_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBT4466615.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="MB022_001616"
     CDS             3854..4591
                     /locus_tag="MB022_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBT4466616.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="MB022_001617"
     CDS             4716..5195
                     /locus_tag="MB022_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBT4466617.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="MB022_001618"
     CDS             5192..5959
                     /locus_tag="MB022_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466618.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="MB022_001619"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="MB022_001619"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4466619.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="MB022_001620"
     CDS             complement(6862..7464)
                     /locus_tag="MB022_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4466620.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="MB022_001621"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="MB022_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="MB022_001622"
     CDS             8145..8729
                     /locus_tag="MB022_001622"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBT4466621.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="MB022_001623"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="MB022_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBT4466622.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="MB022_001624"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="MB022_001624"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBT4466623.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="MB022_001625"
     CDS             10630..10809
                     /locus_tag="MB022_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466624.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="MB022_001626"
     CDS             10831..11856
                     /locus_tag="MB022_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBT4466625.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="MB022_001627"
     CDS             12031..13275
                     /locus_tag="MB022_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBT4466626.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="MB022_001628"
     CDS             13428..14375
                     /locus_tag="MB022_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4466627.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="MB022_001629"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="MB022_001629"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBT4466628.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="MB022_001630"
     CDS             complement(15277..15519)
                     /locus_tag="MB022_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466629.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="MB022_001631"
     tRNA            15640..15712
                     /locus_tag="MB022_001631"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="MB022_001632"
     CDS             complement(15831..16469)
                     /locus_tag="MB022_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4466630.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="MB022_001633"
     CDS             complement(16592..17224)
                     /locus_tag="MB022_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4466631.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5915 a         3076 c         3438 g         4915 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaac
//
LOCUS       DAGCXG010000047        16586 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGCXG010000047 DAGCXG010000000
VERSION     DAGCXG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.050"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..347
                     /locus_tag="MB022_001634"
     CDS             <1..347
                     /locus_tag="MB022_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4466632.1"
                     /translation="LEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMS
                     RKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQS
                     LVQFRKTTVTVV"
     gene            536..1204
                     /locus_tag="MB022_001635"
     CDS             536..1204
                     /locus_tag="MB022_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBT4466633.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1404..1790)
                     /locus_tag="MB022_001636"
     CDS             complement(1404..1790)
                     /locus_tag="MB022_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466634.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1762..3144)
                     /locus_tag="MB022_001637"
     CDS             complement(1762..3144)
                     /locus_tag="MB022_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4466635.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3300..4487)
                     /locus_tag="MB022_001638"
     CDS             complement(3300..4487)
                     /locus_tag="MB022_001638"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBT4466636.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4494..5525)
                     /locus_tag="MB022_001639"
     CDS             complement(4494..5525)
                     /locus_tag="MB022_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4466637.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5541..6995)
                     /locus_tag="MB022_001640"
     CDS             complement(5541..6995)
                     /locus_tag="MB022_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4466638.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7170..8075
                     /locus_tag="MB022_001641"
     CDS             7170..8075
                     /locus_tag="MB022_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4466639.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYVEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8166..8843
                     /locus_tag="MB022_001642"
     CDS             8166..8843
                     /locus_tag="MB022_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4466640.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8840..9010
                     /locus_tag="MB022_001643"
     CDS             8840..9010
                     /locus_tag="MB022_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466641.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9124..10020
                     /locus_tag="MB022_001644"
     CDS             9124..10020
                     /locus_tag="MB022_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4466642.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10075..11445)
                     /locus_tag="MB022_001645"
     CDS             complement(10075..11445)
                     /locus_tag="MB022_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4466643.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11683..12429
                     /locus_tag="MB022_001646"
     CDS             11683..12429
                     /locus_tag="MB022_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4466644.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12426..13265
                     /locus_tag="MB022_001647"
     CDS             12426..13265
                     /locus_tag="MB022_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4466645.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13266..14216
                     /locus_tag="MB022_001648"
     CDS             13266..14216
                     /locus_tag="MB022_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4466646.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14357..15109
                     /locus_tag="MB022_001649"
     CDS             14357..15109
                     /locus_tag="MB022_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466647.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15128..16279
                     /gene="queG"
                     /locus_tag="MB022_001650"
     CDS             15128..16279
                     /gene="queG"
                     /locus_tag="MB022_001650"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBT4466648.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5396 a         3076 c         3316 g         4798 t
ORIGIN      
        1 gtttggagga agctatccag accactaacg attgtcccta tcgtcgtacg tttcactcag
       61 atcaaggttg ggcgtatcaa atgaaagctt atagaaacaa attgaaggag tacaaaatct
      121 ttcaaagcat gtctcggaaa gggaactgct tagataattc acttatggag aatttttttg
      181 gcttattaaa acaagagatt tttcacggga aagtttataa ctgtttcgag gaattaaaat
      241 cagccatcga tagctatatc tattactaca ataatgaacg gataaaacag aaattaaact
      301 ggcaaagtct cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta
      361 gagcataaaa aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac
      421 tacagtgtca cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc
      481 gtgttcgtga gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat
      541 tttttcaatt acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt
      601 gcttgggaag aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt
      661 aggtgggatc ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt
      721 tgccattgcg ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga
      781 aaaggtcaat cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa
      841 catgacagag ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca
      901 gcaagaatgc ttttctcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat
      961 gagtgtattg aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg
     1021 gcaaattcag cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa
     1081 cttgaagaaa gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga
     1141 aaaagggctc tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg
     1201 atagatagac agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt
     1261 tttcacaacc tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag
     1321 ccaaaattcc acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag
     1381 ttgatcactt ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca
     1441 atataccgta cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag
     1501 gcttcaccaa tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa
     1561 ttgagctgaa tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt
     1621 tcttgcaatg tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt
     1681 ttgataccgt tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc
     1741 cgatctcgat actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc
     1801 ttctaatcca ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg
     1861 tcctccttgc aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat
     1921 cgtatcgaca acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag
     1981 gttaagctgt aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat
     2041 atcctggtag tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct
     2101 agcatttgtc gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag
     2161 atagcctaaa gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct
     2221 caagcgagtg ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct
     2281 tgtataatct ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc
     2341 ttgtcccaaa aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac
     2401 ccctagcttg cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat
     2461 acccatttgt ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc
     2521 tgagattttc cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt
     2581 tccgtctttt gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc
     2641 tatagtcgtg tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc
     2701 gtacgcattt gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc
     2761 gacggaatag acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc
     2821 tctgttcttt tccatataat aggccatcct tggggcaaca atatataaat cacttggata
     2881 ggggaaggga agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc
     2941 aggactggaa gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga
     3001 catgactact aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc
     3061 gtaaaaagat ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa
     3121 taccggattt tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt
     3181 ctctttccgt gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg
     3241 aaattgctga gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat
     3301 caaacatgaa cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa
     3361 aattccggag catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca
     3421 ggtatatttt cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt
     3481 cgtgcaccta agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc
     3541 ccagttactt cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat
     3601 gcttctaagt tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa
     3661 acggcatggc gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca
     3721 aattcttctt catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc
     3781 aaggcttctt cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc
     3841 ttattcgtat tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat
     3901 ttcagcgtat ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac
     3961 tgatccatga tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg
     4021 actagttcca agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat
     4081 aattctaatg aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac
     4141 aactcaaacc catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt
     4201 acgtaatttg cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg
     4261 acaccgatat cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct
     4321 gctacacctt gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct
     4381 gtgtgttcac caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt
     4441 ccgaatgttt cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc
     4501 tttcttgttt tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga
     4561 atcgaaccaa atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg
     4621 ccaccgtgat gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca
     4681 gtgaaaataa ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg
     4741 ctagtcaagc gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg
     4801 attccgtcaa caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta
     4861 ggtatttgaa aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta
     4921 tctgttcgca aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac
     4981 tgagaagcat ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct
     5041 tggtcactgg tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg
     5101 atttccacat ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa
     5161 atgacagaag cttcattttc gccattcaaa atgacataag accatttttt taattcaaat
     5221 ccaggagcac caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt
     5281 tttccattga gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca
     5341 ccgatatacg gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct
     5401 cgatcatttg attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc
     5461 ttgtttttgt tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc
     5521 ttcattttca agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc
     5581 attgtatcag ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc
     5641 tctgtcaatg taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga
     5701 gcatagaatg cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta
     5761 gcattaccag tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca
     5821 ccagcgattt tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca
     5881 tttttccatt gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc
     5941 aattgttgag ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct
     6001 gcgatcgtaa agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag
     6061 cgacgagaga tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag
     6121 gcagcaacta gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag
     6181 tagaataata cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca
     6241 agccacatca actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt
     6301 tcattttgct gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca
     6361 gtaccgcctg aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca
     6421 atatgtttat gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta
     6481 gtaccatttg cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc
     6541 tttgtttccg tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa
     6601 aaactatcca ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga
     6661 gtagctaagc caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg
     6721 ccgataacta accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca
     6781 ccaatcaaag gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact
     6841 gtagcaatca tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg
     6901 aataccataa aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa
     6961 gcgtaggaca cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat
     7021 atataatact aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt
     7081 aacaagaaat cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca
     7141 ttatactaaa ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac
     7201 atcaacaagt agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt
     7261 ttcattcttt tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca
     7321 aaggatctta tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga
     7381 ttcgtccagg agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct
     7441 ggctttcatt tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag
     7501 accaatacac gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca
     7561 tgaaagatac ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt
     7621 ttttatcagt ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt
     7681 cctctttagc tgttgaaaca gtcaaataca tcgaggcaca ttatgttgaa aaattgtctg
     7741 ttgaggaaat cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa
     7801 atcatatggg taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt
     7861 ttcttctttc tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata
     7921 gtctagtcgt atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc
     7981 gtaaacgtat gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt
     8041 tggaaaaaca aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat
     8101 cggcaaaaga aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa
     8161 aagggatgcg tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc
     8221 aatttttcaa agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt
     8281 atccagacgg aaaaatagat atagagactg aactacagct cttggcccag catgatcgga
     8341 tcattttcca gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag
     8401 ataatgtctt ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga
     8461 caacaggtgt ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg
     8521 aatttttgag gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat
     8581 tcgttttagc acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt
     8641 atcagcagta tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta
     8701 ttcaaagagt acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata
     8761 tcgtagaagc actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag
     8821 aaatgaaagg aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag
     8881 cgaaaaatca gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga
     8941 gatagacgaa cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct
     9001 caactggtga aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt
     9061 gctacaataa gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa
     9121 aaaatgtatg atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa
     9181 aaacaggcag ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc
     9241 atagcaggag gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt
     9301 acgaccataa cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca
     9361 actaaaacaa aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa
     9421 cttcaagcaa tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt
     9481 gaagaatacc agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt
     9541 gctaaccaaa aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt
     9601 gcagcgtatc aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa
     9661 ttatcggcta attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc
     9721 gcctctcttc aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca
     9781 gcagagtcgg tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc
     9841 tctgaaacta ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga
     9901 gaaggtccaa atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg
     9961 aatgggatga gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga
    10021 taaaagcaat aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt
    10081 tccggttctt ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc
    10141 aataatcaat gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa
    10201 aaaagcaagt gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg
    10261 cattttatct gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg
    10321 aaccatacca ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt
    10381 gatgaacatt ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc
    10441 tacgacagga taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt
    10501 tccatttatg gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc
    10561 tgataatgcc gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac
    10621 tttagaaaaa gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat
    10681 actcacaatg ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg
    10741 gacagcttca atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag
    10801 ttcgattcct tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc
    10861 tccagtgaag aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt
    10921 accaaatcca aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac
    10981 tttgatgatc gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc
    11041 cagcataaga aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca
    11101 ataattcata taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa
    11161 aatattactc caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt
    11221 tgcaaaagaa ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa
    11281 atacaaaaag aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat
    11341 ggttgaggag gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg
    11401 gatatgacgg gcactaagac ttcgagttaa ctttttttct tccatacaaa aactcctttc
    11461 gttgttgagt caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg
    11521 ttagccttat tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa
    11581 ttcgaaaaaa actttttcat cattctactt gaaaatgctt tccattttag ttaaactata
    11641 tttaaataaa aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt
    11701 gtaattcaag acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg
    11761 acgataaatc cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca
    11821 tttatgaaat ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga
    11881 cagccggtaa actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat
    11941 ctttcctttc caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga
    12001 attggacaaa gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta
    12061 gggatggaag aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga
    12121 gtttttattg caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc
    12181 gtagggattg atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat
    12241 tcaggaaaaa cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat
    12301 gaggttattc ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca
    12361 tcgaagaacc ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag
    12421 aaaaaatgat ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt
    12481 tggtcacttc catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac
    12541 gagggatgtc tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt
    12601 cttatatgtt tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag
    12661 cagtgattgg tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg
    12721 tctttagctc cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag
    12781 atttgtatca tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga
    12841 cagcactcgt tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga
    12901 tcacatcatt cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt
    12961 atcttttgat gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa
    13021 tcttggtgat tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt
    13081 tactgaaaat gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt
    13141 ttttcagtta tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat
    13201 tttttatcct tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca
    13261 gatagatgaa aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag
    13321 ccggttgcgg aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag
    13381 tcgtggctac caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag
    13441 atatccacag tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag
    13501 acatcaaaaa ggcaagtgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag
    13561 gtaacagctg gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt
    13621 ttgcagttag caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag
    13681 cagatcccca tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac
    13741 gtatattctc tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat
    13801 atgtggaaaa actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag
    13861 agaacaaaaa attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg
    13921 atttgccagc agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa
    13981 tgaaagcgat cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca
    14041 gtgtagactc aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata
    14101 aactgttcac tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga
    14161 tgaaatggaa tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag
    14221 tgcaaggtct agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt
    14281 atgctgtttt tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag
    14341 gaaggaaggt gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt
    14401 ctttcgattg atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga
    14461 agcagaatta gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga
    14521 gctgtacata ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat
    14581 cggttatttt gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa
    14641 cgacgaaata accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt
    14701 aatcaaaaag atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat
    14761 gaatatgttt tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt
    14821 cacgtacaca cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga
    14881 gttttcgcca tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt
    14941 gaattttttt aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt
    15001 atttgctgca agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt
    15061 tgtccagctg aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag
    15121 gaagaccatg acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa
    15181 aatcgggttc accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa
    15241 agcagctggc catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga
    15301 aatgacattt gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat
    15361 ccatgaagaa gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat
    15421 cgattaccat gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag
    15481 agcagaaaaa tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga
    15541 actgatcgat gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt
    15601 gttgatcact gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt
    15661 atttgagcct gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc
    15721 ttgtccgaca aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta
    15781 tcaaactcag acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat
    15841 ttatggatgt gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt
    15901 tcatgaagag atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc
    15961 gatgtctaat aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa
    16021 gaaacctttg cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt
    16081 gcctgagatt tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg
    16141 ggctattgga cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat
    16201 attagctaaa gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt
    16261 gcggagaaag ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg
    16321 ttgtaacaat tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa
    16381 tcaaaaattt ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt
    16441 tttcattcaa aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt
    16501 tatggttttg tatcaaaaaa atatttgttt aaatgttaca aatattaaat aattcgttat
    16561 tttgtcatta aatgtcatgt ttcttt
//
LOCUS       DAGCXG010000048        16487 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGCXG010000048 DAGCXG010000000
VERSION     DAGCXG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.051"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(718..3867)
                     /locus_tag="MB022_001651"
     CDS             complement(718..3867)
                     /locus_tag="MB022_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBT4466649.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="MB022_001652"
     CDS             complement(3880..5079)
                     /locus_tag="MB022_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBT4466650.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="MB022_001653"
     CDS             complement(5069..6664)
                     /locus_tag="MB022_001653"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBT4466651.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7248..7955)
                     /locus_tag="MB022_001654"
     CDS             complement(7248..7955)
                     /locus_tag="MB022_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466652.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8020..8856)
                     /locus_tag="MB022_001655"
     CDS             complement(8020..8856)
                     /locus_tag="MB022_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4466653.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8874..10283)
                     /locus_tag="MB022_001656"
     CDS             complement(8874..10283)
                     /locus_tag="MB022_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4466654.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10297..12120)
                     /locus_tag="MB022_001657"
     CDS             complement(10297..12120)
                     /locus_tag="MB022_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4466655.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12963..13289)
                     /locus_tag="MB022_001658"
     CDS             complement(12963..13289)
                     /locus_tag="MB022_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466656.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13335..13697)
                     /locus_tag="MB022_001659"
     CDS             complement(13335..13697)
                     /locus_tag="MB022_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBT4466657.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13734..13949)
                     /locus_tag="MB022_001660"
     CDS             complement(13734..13949)
                     /locus_tag="MB022_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466658.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14076..14411
                     /locus_tag="MB022_001661"
     CDS             14076..14411
                     /locus_tag="MB022_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466659.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14487..16484)
                     /locus_tag="MB022_001662"
     CDS             complement(14487..16484)
                     /locus_tag="MB022_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466660.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         4902 a         3419 c         2547 g         5619 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
     6841 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
     6901 ttcataaaaa aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt
     6961 cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta
     7021 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
     7081 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
     7141 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
     7201 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatcttttta cccattttca
     7261 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
     7321 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
     7381 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
     7441 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
     7501 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
     7561 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
     7621 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
     7681 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
     7741 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
     7801 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
     7861 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
     7921 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
     7981 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     8041 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     8101 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     8161 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     8221 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     8281 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     8341 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     8401 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     8461 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     8521 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     8581 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     8641 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     8701 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     8761 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     8821 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     8881 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     8941 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     9001 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     9061 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     9121 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     9181 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     9241 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     9301 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     9361 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     9421 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     9481 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     9541 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     9601 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     9661 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     9721 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     9781 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     9841 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     9901 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     9961 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
    10021 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
    10081 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
    10141 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
    10201 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
    10261 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
    10321 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
    10381 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
    10441 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
    10501 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
    10561 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
    10621 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
    10681 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
    10741 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
    10801 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
    10861 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
    10921 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
    10981 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
    11041 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
    11101 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
    11161 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
    11221 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
    11281 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
    11341 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
    11401 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
    11461 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
    11521 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
    11581 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
    11641 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
    11701 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
    11761 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
    11821 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
    11881 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
    11941 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
    12001 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
    12061 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
    12121 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
    12181 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
    12241 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
    12301 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
    12361 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
    12421 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
    12481 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
    12541 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
    12601 cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcaata gaatgatttg
    12661 ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat
    12721 gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat
    12781 tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa attcccgaca
    12841 atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa actataaaaa
    12901 ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta
    12961 aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt
    13021 ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg
    13081 tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg
    13141 tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa tccagatgta
    13201 cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc tataatgaca
    13261 aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgt
    13321 tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct
    13381 acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata taagtagccc
    13441 ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac taccatagaa
    13501 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
    13561 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
    13621 gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat ttctttgcca
    13681 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagttaactg
    13741 tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa
    13801 agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
    13861 tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt ttcttttctg
    13921 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
    13981 aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta caaatacaac
    14041 aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg
    14101 tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct
    14161 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata
    14221 gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
    14281 tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag
    14341 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
    14401 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
    14461 aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt
    14521 actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac tatcccagct
    14581 cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc taaatatatt
    14641 aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca
    14701 agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt tttcacgctg
    14761 ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt ctctgtcatt
    14821 agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
    14881 tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
    14941 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaattttt
    15001 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
    15061 tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt
    15121 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
    15181 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
    15241 ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
    15301 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
    15361 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
    15421 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
    15481 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
    15541 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
    15601 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
    15661 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
    15721 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
    15781 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
    15841 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
    15901 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
    15961 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
    16021 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
    16081 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
    16141 tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc atctttactc
    16201 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
    16261 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
    16321 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
    16381 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
    16441 tcttgatcta cataaattgg aatattaatc attgatgttt ccatttg
//
LOCUS       DAGCXG010000049        16462 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGCXG010000049 DAGCXG010000000
VERSION     DAGCXG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.052"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..121)
                     /locus_tag="MB022_001663"
     CDS             complement(<1..121)
                     /locus_tag="MB022_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBT4466661.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGL"
     gene            complement(369..1205)
                     /locus_tag="MB022_001664"
     CDS             complement(369..1205)
                     /locus_tag="MB022_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBT4466662.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1216..2145)
                     /locus_tag="MB022_001665"
     CDS             complement(1216..2145)
                     /locus_tag="MB022_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBT4466663.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2337..2690
                     /locus_tag="MB022_001666"
     CDS             2337..2690
                     /locus_tag="MB022_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4466664.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2764..3420)
                     /locus_tag="MB022_001667"
     CDS             complement(2764..3420)
                     /locus_tag="MB022_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4466665.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3646..5580
                     /locus_tag="MB022_001668"
     CDS             3646..5580
                     /locus_tag="MB022_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4466666.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5666..6889)
                     /locus_tag="MB022_001669"
     CDS             complement(5666..6889)
                     /locus_tag="MB022_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4466667.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6883..8781)
                     /locus_tag="MB022_001670"
     CDS             complement(6883..8781)
                     /locus_tag="MB022_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBT4466668.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSVIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8775..9893)
                     /locus_tag="MB022_001671"
     CDS             complement(8775..9893)
                     /locus_tag="MB022_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4466669.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9976..10809)
                     /locus_tag="MB022_001672"
     CDS             complement(9976..10809)
                     /locus_tag="MB022_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4466670.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10802..11719)
                     /locus_tag="MB022_001673"
     CDS             complement(10802..11719)
                     /locus_tag="MB022_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466671.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11946..13070)
                     /locus_tag="MB022_001674"
     CDS             complement(11946..13070)
                     /locus_tag="MB022_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4466672.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13253..14161
                     /locus_tag="MB022_001675"
     CDS             13253..14161
                     /locus_tag="MB022_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4466673.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14302..15915
                     /locus_tag="MB022_001676"
     CDS             14302..15915
                     /locus_tag="MB022_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4466674.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5208 a         3224 c         2734 g         5296 t
ORIGIN      
        1 tcaatccctg aacgatctca aatttccaag gacttaataa aattctggat tcttccaaaa
       61 tttctactag cattttttca aaaaccaaac tatcatccaa aataaatata cgcgttacca
      121 taaaaagtac ctccactcga tattttacta tcaaaatcat ttcatacaac ttttcaacat
      181 acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt tgaaaccgat
      241 atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa tacatcatgc
      301 aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg tttatttatt
      361 ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac tttctcagcg
      421 tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc tgattggatg
      481 gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg catatgcgtc
      541 aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg gaacaagaag
      601 atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct gatttttttg
      661 ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata gttttcatct
      721 gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat ttggtctaag
      781 tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt ctgcatctcc
      841 ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc tacgatcgta
      901 gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc tagcagtaca
      961 taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg ctcgtgtgcc
     1021 ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc caaaagagca
     1081 gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata ttgatcgagt
     1141 ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat caaaaaccgc
     1201 agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg caatcagtcc
     1261 gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta gatttttgat
     1321 atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga acagacttaa
     1381 caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa aggccaataa
     1441 cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa ttctagttgg
     1501 taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac gacctgtatc
     1561 aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca gctgattttt
     1621 tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat tttctgctac
     1681 tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa gccagacacc
     1741 aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct gccaagaatt
     1801 gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag cagtcagcat
     1861 aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa cgatcggtac
     1921 gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa
     1981 gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata cttgtaaggc
     2041 gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga tcgataagaa
     2101 aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt tcctcctttt
     2161 tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac aagttgctct
     2221 gtaaattttt acacaactct tattttttac caaagcactt caattgactt tttctgttat
     2281 tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga ataaaaatga
     2341 aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc gttgccgtca
     2401 tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac
     2461 ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca ccagaagtag
     2521 ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag
     2581 tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta gatgaactga
     2641 cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa tcgttgtcca
     2701 aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa tcgcttttta
     2761 tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa
     2821 ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg gagcattcaa
     2881 tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact gattgactac
     2941 ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt ggatcttgat
     3001 ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc gcccaacgat
     3061 ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat ggaatttgta
     3121 ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg tcaattgatc
     3181 gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac catacccacg
     3241 ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat ctacttgaac
     3301 ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta aaatccttaa
     3361 atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat gatctttcac
     3421 gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata gcagtattta
     3481 taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta ttcatttatt
     3541 tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta attgaaaaat
     3601 agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat attattacaa
     3661 gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat acatttagaa
     3721 atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa atcgacttta
     3781 ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa aaataaaaca
     3841 gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac agtttggaac
     3901 gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg cgagctagag
     3961 attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt aaaagaatac
     4021 gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa tgaaattcgt
     4081 tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat cgatacttta
     4141 tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca ccctgacatt
     4201 ttgattctag acgaaccaac gaaccattta gatatcgaga cactttcctg gcttgagaat
     4261 tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta ttttttagat
     4321 aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta caaaggtaac
     4381 tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa
     4441 aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct tgttcgcgcc
     4501 tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga ccggctggat
     4561 cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa gccttctgga
     4621 aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac cttatctgaa
     4681 ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc
     4741 gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg tggaaaagaa
     4801 acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga tctgcataca
     4861 aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga aaaagagatc
     4921 cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc aatccctctg
     4981 ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa caaagaaaat
     5041 tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga agtattagaa
     5101 aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg atatttcatc
     5161 aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga
     5221 gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga attgttggca
     5281 caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa
     5341 gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg
     5401 gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg
     5461 gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa
     5521 gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa
     5581 cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca
     5641 aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca
     5701 ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt
     5761 tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc
     5821 tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt
     5881 tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc accatactag
     5941 atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat
     6001 cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt
     6061 gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag
     6121 cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt
     6181 tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg
     6241 cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg
     6301 cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag
     6361 gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta
     6421 atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat
     6481 ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac
     6541 agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat
     6601 cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa
     6661 tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac
     6721 tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat
     6781 tccattcttt ttccattaat tctttataac attcatttat ccatttttct ggatgtatta
     6841 tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct
     6901 ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg
     6961 acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc
     7021 gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca
     7081 tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc
     7141 gtcacttaaa cagataataa ctgaaaagcc agcttcactc accctatagt tttccttgtc
     7201 ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata
     7261 tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg
     7321 aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc
     7381 tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat
     7441 gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga
     7501 atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact
     7561 agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg
     7621 tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga
     7681 aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg
     7741 gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact
     7801 attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt
     7861 ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta accccttgat
     7921 ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac
     7981 tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa
     8041 atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt
     8101 ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata
     8161 atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat
     8221 attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat
     8281 tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat
     8341 ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa
     8401 ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct
     8461 ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga
     8521 tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa
     8581 aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat
     8641 ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt
     8701 aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt
     8761 ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca
     8821 ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct atggtatgct
     8881 tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct
     8941 gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca
     9001 tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc
     9061 gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata
     9121 aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa
     9181 cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg
     9241 tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata
     9301 tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga
     9361 gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa
     9421 ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag
     9481 ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga
     9541 ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct
     9601 acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt
     9661 attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca
     9721 ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt
     9781 ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt
     9841 tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc
     9901 ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa
     9961 cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg
    10021 cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc
    10081 gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac
    10141 cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc
    10201 cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt
    10261 cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg
    10321 aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat
    10381 gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat
    10441 caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt
    10501 ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctga
    10561 aactgtactg attacagcaa taacaatcga attccaaata atcaagggat aattatatct
    10621 cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc
    10681 acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa
    10741 aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg
    10801 tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa
    10861 acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt
    10921 tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga
    10981 atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta
    11041 aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc
    11101 catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata
    11161 aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc
    11221 aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga
    11281 ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa
    11341 tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca
    11401 ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa
    11461 tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt
    11521 caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa
    11581 tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag
    11641 cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg
    11701 gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt
    11761 acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct
    11821 gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct
    11881 ggtaaaacag tatttgatgt attttcattg attttattca atagttcata gtatttttgt
    11941 cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat
    12001 gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta
    12061 agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct
    12121 cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc
    12181 catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca
    12241 acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca
    12301 atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc
    12361 agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata
    12421 aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt
    12481 aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct
    12541 atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa
    12601 ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac
    12661 tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat
    12721 ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg
    12781 tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca
    12841 atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca
    12901 tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt
    12961 ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat
    13021 aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct
    13081 tctttctttt caagttctta tataaaacgc tttcacttat gtatattaac ggaattagta
    13141 acacaaatat ttatataaaa aaatatttta cttgtcgttt tggcttttca tttttgttat
    13201 aatagtcatt aattataaat agaaagcttt ttatataaaa agagaaagga aagtggttct
    13261 tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa acactaacct attccataga
    13321 tacccatctt catttatctg ctgctcccta ttttcgacta ctaataaatg aaagcgctaa
    13381 tttatatgct gaatttgcac ataaatctat agaactagat aatccttccc ttttattcat
    13441 tgataaacag caagaagtcc aaatcttttc tactcaaaag gaaacacaaa cttttatcat
    13501 tatccagttt tattttgatg agcaattatt agcacatatt acgggacaat ctttttctat
    13561 agatcaagaa ataacatcta tcattacttt tctgaaaaat gaacagttca aaattgatta
    13621 tctaaaacat agtaagccag ctatttccta tgatcaggaa atcaattctt ttttcatact
    13681 atgccaacag atgagcattc atttgctttc cttattagtt acttattgtc tgcaaaaaat
    13741 tcaattgtct tccttaccaa ggcattatca acaggaactt gctttattga caataccttc
    13801 aaatgagaaa gtcattccta aaaaaaagca tattctttat tcagatgaat tagttgatcc
    13861 aatcatacga tatttagaag aacatctttc agaaaatttt ttgcttgaac aattagccca
    13921 aatttttttt atcagtccag ccagcttaaa aaaacatttt agaaaagtga atggaaaaag
    13981 tgttattagc tattttaaag aactaaaaat ggaaaaagca aaacgtctcg tgctagaaaa
    14041 tgaattaacc tatacagaaa ttgcagatat attaggattt cattcgatcc atcatttttc
    14101 ttcagctttt aaaaaatata cagggttatc cccttcaaag tattatctat ccttaaaata
    14161 gaaaaaaatt tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa
    14221 taaacaataa ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa
    14281 cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct
    14341 tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc
    14401 ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact
    14461 gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat
    14521 gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa
    14581 gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt
    14641 gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc
    14701 cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat
    14761 gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc
    14821 ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa
    14881 attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca
    14941 agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt
    15001 tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca
    15061 accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat
    15121 ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag
    15181 ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt
    15241 ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt
    15301 gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca
    15361 aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca
    15421 agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca
    15481 tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac
    15541 ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc
    15601 cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa
    15661 acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata
    15721 cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat
    15781 gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga
    15841 agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt
    15901 ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac
    15961 ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa
    16021 gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa
    16081 gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt
    16141 gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac
    16201 gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt
    16261 ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt
    16321 tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg
    16381 ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt
    16441 taattttttt gttcctattt at
//
LOCUS       DAGCXG010000050        16207 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGCXG010000050 DAGCXG010000000
VERSION     DAGCXG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.053"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..452)
                     /locus_tag="MB022_001677"
     CDS             complement(<1..452)
                     /locus_tag="MB022_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4466675.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
     gene            complement(557..1420)
                     /locus_tag="MB022_001678"
     CDS             complement(557..1420)
                     /locus_tag="MB022_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4466676.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1616..3715)
                     /locus_tag="MB022_001679"
     CDS             complement(1616..3715)
                     /locus_tag="MB022_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4466677.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3935..5044)
                     /gene="rpoD"
                     /locus_tag="MB022_001680"
     CDS             complement(3935..5044)
                     /gene="rpoD"
                     /locus_tag="MB022_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBT4466678.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5068..6909)
                     /locus_tag="MB022_001681"
     CDS             complement(5068..6909)
                     /locus_tag="MB022_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBT4466679.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7091..9406)
                     /locus_tag="MB022_001682"
     CDS             complement(7091..9406)
                     /locus_tag="MB022_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4466680.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9591..10451)
                     /locus_tag="MB022_001683"
     CDS             complement(9591..10451)
                     /locus_tag="MB022_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBT4466681.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10510..11031)
                     /locus_tag="MB022_001684"
     CDS             complement(10510..11031)
                     /locus_tag="MB022_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBT4466682.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11309..12070
                     /locus_tag="MB022_001685"
     CDS             11309..12070
                     /locus_tag="MB022_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4466683.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12096..14687
                     /gene="mprF"
                     /locus_tag="MB022_001686"
     CDS             12096..14687
                     /gene="mprF"
                     /locus_tag="MB022_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4466684.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14875..16062)
                     /gene="tuf"
                     /locus_tag="MB022_001687"
     CDS             complement(14875..16062)
                     /gene="tuf"
                     /locus_tag="MB022_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4466685.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         4383 a         3398 c         2829 g         5597 t
ORIGIN      
        1 tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc ttttactaaa
       61 aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc ttctacttcc
      121 tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc gccttctttt
      181 ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat tttcaattct
      241 gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact cttttttttc
      301 actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa aagcacaagc
      361 agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg ctgttttgtt
      421 tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat ttataataat
      481 tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct agttaagaaa
      541 aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg ttttcttgtg
      601 taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct ttgctgatac
      661 caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt tgctgatctg
      721 cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct tcatatgctc
      781 ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact cgacctttta
      841 tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac ccaagataga
      901 aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt cctatcacat
      961 ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt ttttcatctg
     1021 ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc aagcgatccc
     1081 cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg acaaagtctt
     1141 tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca gtaacgattc
     1201 caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga ttttcatgtt
     1261 tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca ccttctgatt
     1321 tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca ttttcatcaa
     1381 taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc ctctatagtg
     1441 aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac ttgaatagct
     1501 gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca tactcccctt
     1561 tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc taaaattaat
     1621 tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg taaaatggca
     1681 aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca tatagattat
     1741 aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct tcaacgatat
     1801 atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc cacgtccatg
     1861 ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc tgattcgctg
     1921 agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt gcgaccgcag
     1981 cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct tgttcttcag
     2041 ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc tctttatcag
     2101 tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga tctgtatttt
     2161 tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct gaatccacta
     2221 cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat tcagcatatt
     2281 gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg ttatccgttt
     2341 cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga tgatcatcga
     2401 cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc ttagattgga
     2461 tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga ctggataaat
     2521 cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat tgatggtgat
     2581 catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc tgtctttttg
     2641 cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc cagcttcctc
     2701 caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat tttttctttt
     2761 gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt tcagtatctg
     2821 gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc tcttcttttt
     2881 ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca gcatcggatt
     2941 cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt atttcttttt
     3001 tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca gtaaatactt
     3061 cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct ttacctgtgg
     3121 cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct actttgtttt
     3181 cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct tcttttgtat
     3241 cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca ttatcgacga
     3301 tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct tcttgattct
     3361 ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt ttctctatat
     3421 tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt tcgaataatt
     3481 ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga tcgtttaatt
     3541 ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca tctgtatttg
     3601 tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga ccgcagtctg
     3661 gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt ttcatcactt
     3721 agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt ctatacgtca
     3781 cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc ttcctataga
     3841 aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa accaaacagt
     3901 tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct tttaattgtt
     3961 ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga cgaatacgtt
     4021 cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg ccatcatcta
     4081 aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct agtacatctt
     4141 ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt gcttcctggt
     4201 cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt tcaagggaaa
     4261 caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga tccatttccg
     4321 caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga cgttggatac
     4381 gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta cgtgcttggt
     4441 cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt gaaaacttaa
     4501 atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt ccttcttgga
     4561 tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcaatactt actactaaac
     4621 gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg attttcaatg
     4681 ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct ttcagataca
     4741 tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct gtttttgctt
     4801 gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca ttttcatcca
     4861 ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca tctgcattca
     4921 aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt cgtttgggtt
     4981 tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct gttccatttg
     5041 ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt tcgttaaatt
     5101 gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc tggcttcttg
     5161 ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga tcacatctag
     5221 caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt cgatttccac
     5281 caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa ggttgaattc
     5341 ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg catcatgaat
     5401 aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa atgcacgata
     5461 taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc
     5521 ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt gctgatttgt
     5581 ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa actctgttga
     5641 cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa gttcaactag
     5701 taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc cttgttttaa
     5761 ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt cttggaaaga
     5821 tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa cgactacgac
     5881 agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg ttgcttcaaa
     5941 acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt ccatttgttg
     6001 gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga taccagcctg
     6061 ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt ttttccggat
     6121 atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca gttctgtttc
     6181 tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg caagccagcg
     6241 tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca ctctttgata
     6301 aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc gttctaacaa
     6361 ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct cattaggtgc
     6421 aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt gcaaatattc
     6481 taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt
     6541 ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag ctatagtacg
     6601 ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta ccactgcttc
     6661 tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc ctttaccaca
     6721 accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc
     6781 atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga catattgtcc
     6841 aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt gaggaatccg
     6901 ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca gaagatattt
     6961 caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt aaaggacaca
     7021 attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca catttctatc
     7081 tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca gccttccagt
     7141 acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct acgctctctc
     7201 ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca tatatcccta
     7261 cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa gcttttacaa
     7321 ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt tcagcatttc
     7381 cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact acaagcaagg
     7441 catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt tttcttttat
     7501 cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca atcagcgtga
     7561 tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc gataaaataa
     7621 atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg ttgaccactc
     7681 ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac aataccagat
     7741 tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa tctgcttctc
     7801 ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt
     7861 tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa accgtatagc
     7921 gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg acatacgcat
     7981 cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga ttgtctccag
     8041 aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct tgagcgtttt
     8101 ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct ggtttttctt
     8161 cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca ttcgcttggg
     8221 ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc gctcctacgt
     8281 acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa gaaaatgcga
     8341 ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata gcccgcatag
     8401 tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg atggctttct
     8461 cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca gtaatggtcc
     8521 ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa tacatgtctt
     8581 gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca atacttatta
     8641 gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca ataacagaaa
     8701 ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa
     8761 taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt tcagaacgaa
     8821 tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct gcacctactc
     8881 ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc aattcagctc
     8941 cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt gcttctgccc
     9001 gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt tttaccaaat
     9061 ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg atcactagta
     9121 cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt atttcttgat
     9181 aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg gaatacgcac
     9241 ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac gtcaatatat
     9301 tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca ttttgctggc
     9361 cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc tttttctcca
     9421 ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc aagattaaaa
     9481 acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa aaaagatgcc
     9541 aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct ttacttatat
     9601 tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga
     9661 caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt
     9721 gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg
     9781 taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca
     9841 atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac
     9901 accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg
     9961 gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgtat
    10021 aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct
    10081 cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca
    10141 aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt
    10201 agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg
    10261 aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct
    10321 gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc
    10381 acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc
    10441 cttttttcca ctatttgcgc actccttctt tcatttaatt ttattcatga ttgaacattt
    10501 cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac
    10561 aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa
    10621 ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag
    10681 cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac
    10741 ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc
    10801 ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc
    10861 agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc
    10921 tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc
    10981 catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac
    11041 cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat
    11101 gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt
    11161 tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct
    11221 gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc
    11281 gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt
    11341 aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta
    11401 ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg
    11461 ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga
    11521 tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc
    11581 cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt
    11641 tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg
    11701 attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat
    11761 cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa
    11821 aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa
    11881 aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca
    11941 ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg
    12001 acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc
    12061 tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc
    12121 tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg
    12181 tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg
    12241 ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac
    12301 ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt
    12361 acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt
    12421 ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga
    12481 tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc
    12541 tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat
    12601 ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga
    12661 aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt
    12721 tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga
    12781 ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta
    12841 tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg
    12901 gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca
    12961 ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc
    13021 gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct
    13081 tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag
    13141 agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta
    13201 gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac
    13261 gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg
    13321 attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta
    13381 aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa
    13441 tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt
    13501 tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt
    13561 tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc
    13621 ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc
    13681 ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt
    13741 ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata
    13801 aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag
    13861 cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag
    13921 agattgttat gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt
    13981 atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct
    14041 tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg
    14101 agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag
    14161 ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca
    14221 aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata
    14281 aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg
    14341 atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt
    14401 tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga
    14461 ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat
    14521 ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt
    14581 ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac
    14641 aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg
    14701 atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc
    14761 tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg
    14821 accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc
    14881 ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt
    14941 cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc
    15001 aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg
    15061 gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt
    15121 tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc
    15181 aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt
    15241 atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc
    15301 tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc
    15361 acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac
    15421 tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt
    15481 atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc
    15541 ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg
    15601 aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa
    15661 gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg
    15721 aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc
    15781 tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg
    15841 tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc
    15901 tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac
    15961 tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat
    16021 gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa
    16081 ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact
    16141 aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg
    16201 tcaatat
//
LOCUS       DAGCXG010000051        16170 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGCXG010000051 DAGCXG010000000
VERSION     DAGCXG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.054"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            84..551
                     /locus_tag="MB022_001688"
     CDS             84..551
                     /locus_tag="MB022_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBT4466686.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            558..3050
                     /locus_tag="MB022_001689"
     CDS             558..3050
                     /locus_tag="MB022_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBT4466687.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            3271..4194
                     /locus_tag="MB022_001690"
     CDS             3271..4194
                     /locus_tag="MB022_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4466688.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            4200..5453
                     /locus_tag="MB022_001691"
     CDS             4200..5453
                     /locus_tag="MB022_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4466689.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            5587..6933
                     /gene="gor"
                     /locus_tag="MB022_001692"
     CDS             5587..6933
                     /gene="gor"
                     /locus_tag="MB022_001692"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBT4466690.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(7747..8700)
                     /gene="menA"
                     /locus_tag="MB022_001693"
     CDS             complement(7747..8700)
                     /gene="menA"
                     /locus_tag="MB022_001693"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBT4466691.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(8713..9816)
                     /locus_tag="MB022_001694"
     CDS             complement(8713..9816)
                     /locus_tag="MB022_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBT4466692.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(9906..10862)
                     /locus_tag="MB022_001695"
     CDS             complement(9906..10862)
                     /locus_tag="MB022_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBT4466693.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            11143..13077
                     /locus_tag="MB022_001696"
     CDS             11143..13077
                     /locus_tag="MB022_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4466694.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            13534..13950
                     /locus_tag="MB022_001697"
     CDS             13534..13950
                     /locus_tag="MB022_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4466695.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            13964..14530
                     /locus_tag="MB022_001698"
     CDS             13964..14530
                     /locus_tag="MB022_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBT4466696.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            14553..15539
                     /locus_tag="MB022_001699"
     CDS             14553..15539
                     /locus_tag="MB022_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4466697.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15694..>16170)
                     /gene="scm"
                     /locus_tag="MB022_001700"
     CDS             complement(15694..>16170)
                     /gene="scm"
                     /locus_tag="MB022_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBT4466698.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         5161 a         2883 c         3385 g         4741 t
ORIGIN      
        1 atttaacaaa aagcgttctt ctttctttct attatcaaat tcgttataat taaggtcaat
       61 attagtcaaa gggaatgaat aagatggcac atcagaatac ctcggattta attgaatcat
      121 atataaagaa aattctggaa aatagtgaaa tgatcgagat tcgaaggatt gaaatggcag
      181 acctctttaa ctgtgttcct tcacaaatca actatgtgat taatacccgt tttaccatcc
      241 aacgtggtta tctagtagaa agcaaacgtg gtggtggtgg ttatatacga attgcaaaag
      301 taaggatttc agataaaaaa caattacttg agcagataaa tcagttattt gatgatacaa
      361 tcagtgagaa aaatgctttt gctattattc aaaaacttta tgaagaccaa ataattacaa
      421 aaaaagaagg aaacttgatg ttgagtgcaa ttgcaaaaaa cacattgaat ttcaatgaat
      481 atgaagatca tacacgtgct cggattctcc gagcatttct ggaacgttta agttatgaag
      541 atgggaagtg agattagatg gaagaacttt ttactgaaag agcccaagca gtattagaaa
      601 ttgcacaaga agaagcaaaa cggttgaaac atcaatcagt tagttcagaa catgttttac
      661 tagccttagt ggtcgagcaa aatggaattg ctggaaaagt attacgtgaa atggatttga
      721 atgaaacgga aatttatgaa gaaattgaac atctgattgg ttatggtggt atccgttctt
      781 accctaaagg agtctttctt ccatattcac caagaatgaa acaagttttt gctttagcta
      841 gcagcgaagt gaagaaaact ggttctcaaa aagttggaac agaacatatt ttgatgagct
      901 tattaaaaga tgaaagtatc atggctactc gaatcatgat caacttaggt atcagccttt
      961 caaaagcccg tcaagttctg aaacaaaaaa tggggctagc tggtgattct gctggaaaag
     1021 gaatgaatcg tcgtagaaat gtaaatgtac aagataaacg gcaaacaccg caaggaacac
     1081 ctacgctaga ttcattagca cgtgatttga cgaaattagc aaaagaaaac aagctggacc
     1141 cagttgttgg tcgttcgaga gaagtgaaac ggcttatcca gattcttagc cgacggacaa
     1201 agaataaccc tgtactggtg ggtgaacctg gtgtaggcaa aacagccatt gctgaagggt
     1261 tagctcaaaa aatcatttta ggtgaagtgc cagaagacat gcaagaaaag cgcctgatga
     1321 tgcttgatat gggatctttg gttgctggta cgaaatatcg cggtgaattt gaagatcgaa
     1381 tgaaaaaggt catcgacgaa atctataatg atggtcaagt aatcttattc attgatgaat
     1441 tgcatacctt aattggtgca ggtggagcag aaggagcaat tgatgcttcg aatatcttga
     1501 aaccggcgtt agctcgagga gaattacaaa cgatcggagc aacgacccta gatgaatatc
     1561 aaaaatatat cgaaaaagat tctgcattag agcgacgttt tgctcgcatt caagtagatg
     1621 agccaactcc tgaagaagca gaagtgattc ttcaaggatt acgtacaaga tatgaagagc
     1681 accatggagt ggagatcaca gatgaagctg tacgagcagc agtcaatctt tctgttcgtt
     1741 atatcacttc tcgtcaacta cccgataaag caattgattt gatcgatgaa tcagcagcga
     1801 aagtccgttt ggatcaaacc gatcacttaa caaaatcaac tgtgatcaaa ctagaaatcg
     1861 atgaactagt acaagaaaag gaagcagcga tccaaaaaca agactttgaa aatgctgctc
     1921 aattacgcag acaagaaaaa gcactacgta aaaagctgca aaaagtttct gcaattgaag
     1981 caaaacagga gcaaggttat tctgatcgtg taacagaaga agatgttgct acagttgtat
     2041 cggagtggac tggtgtccct ctacagcagt tggagaaaaa agaaagcgaa cgcttgttag
     2101 aactggaagg acttctgcat gaacgtgtcg ttggtcaaga tgaggctgtc aaagcagttt
     2161 cacgtgcgat ccgccgtgca cgaagcggat tgaaagatcc tgatcgtcca attggttcat
     2221 tcatgttcct tggaccaact ggtgtcggaa aaacagaatt ggccaaagca ctttcagaag
     2281 tcatgtttgg aagtgaagat gccctgatcc gtgttgatat gtcagaattt atggaaaaat
     2341 acagtacaag ccgattaatc gggtctcctc caggatacgt agggtatgat gaaggtggac
     2401 agttgacaga aaaaattcgt caaaaaccat attcagtgat tctcttagat gaagtggaga
     2461 aagcacatcc agacgttttc aatttacttc tgcaagtatt ggatgatggt catttaacag
     2521 attctaaagg tagaaaagtg gactttagaa atacaatcat gattatgact tcaaatatcg
     2581 gagcgacaca aattcgagaa gaaaaaaatg tcggattcaa tgtccaagat gttactaaag
     2641 atcacaaagc catgcaaaaa cgtatcttag aagagttaaa gaaagctttc cgacctgaat
     2701 tcttgaatcg aatcgatgag actgtcgtct tccattcatt gagtaaagat gaaatccata
     2761 caatcgtcca aatcatgagc aaagcgatta tcaaacgatt gaaagaacaa gatattcaag
     2821 tgaaaatcac accatctgca atcgatgtga tcgggaaagc tggatttgat cctgaatatg
     2881 gtgcacgtcc aatccgtcgc gcacttcaaa aagaaattga agatcgattg agcgaggctt
     2941 tgcttggcgg agaaattcgt ttaggtgacc acgtaacagt aggtgcttca aaaggtaaga
     3001 tcacattgaa tgttcgcgga cctaaaaagg aaacagtagg ttccctatag ttacttggcc
     3061 tgtgtagctg aatttttata atagtaagac ttgattaaga ggttgtgaca agatttttgt
     3121 cacaatctct ttttatattt ggacagaata tatcgaactt tattttgatt ttttgtgaaa
     3181 taattttcaa aaatccgact ttaaatagaa gaagtattgg aaagttttta gagctatgct
     3241 actattatga agttgtagga ggagaaaatt atgatcgaac tcataaccac agtagaaact
     3301 attgaacaag cagaacagtt gcttgctgca ggtgcagata cactatattt cggtgaagag
     3361 caatttggat tgcgtctgcc tgcttcattt acacgagaag aacagcgaca attggtagaa
     3421 ttagcacaca agtatggtaa aaaagcgaat attgctgtaa atgggatcat tcatcctgaa
     3481 aaaatgaaaa atgtacctga atatttggct tttttaaaag aaataaacgt agatgtgatc
     3541 acagttggtg atacaggtat tatttatacg atgaaaaaaa atcctgaatt gcgtataccg
     3601 tatatttatg atgctgaaac attagtaacg agcgcacgtc aaatcaattt ctgggcgaaa
     3661 aaaggcgcag ttggagcagt acttgctcgt gaagttcctt ttgaagagat gaaggcgatg
     3721 gaagaaaaac tcgatattcc tgtagaaacc cttgtgtatg gtgctacatg catccatcaa
     3781 tccaaacgtc cactgctaca gaattactat aactatacga aacaagacga acaaaaagat
     3841 cgtgaaagag gtctttttat ctcggaacct aaaaaagaag agacacatta ttctatttat
     3901 gaagatagtc atggcacaca tatttttgcc agcaatgatc tgaatctctc gaatgaattg
     3961 aatgaacttt atcagcatca ttactgtaca tggaaattgg acggattatt tactccaggc
     4021 gaaaaattcg taaacatcac tcgactattc gccgaagcaa aaaaacaaat tttagaaggt
     4081 agctgggatc aagaaaaagc ggaaaaaatg actagcgaaa tcagtaaatt gcatcctgaa
     4141 aaccgtgaat tagatacagg attcttttat attgatcctt ctgctatcaa ataaggagta
     4201 tgatgaaaga aatgacagta aaaagaaaat tgaaacgacc agaaatccta gcgccagcag
     4261 gaacactgga aaagttaaaa accgcgatcc attatggagc agatgcagta tatattggcg
     4321 ggaatgcata tggtttaaga agccgtgcag gtaattttac ttatgaagaa atggctgaag
     4381 gagttgcttt tgcaaaagaa catgatgcga aggtatacgt tgctgctaat atggtcaccc
     4441 atgaaggaaa tgaagatgga gcaggggaat ttttccgcag tttgcgtgat gtcggtattt
     4501 ctgcagtgat tgtttctgat ccagctttga tcgaaatttg tgcgacagaa gccccaggtc
     4561 tgccgatcca tttatccact caagcttcag caactaattt tgagacattg gaattctgga
     4621 aagaagaagg gttggaacgt gtcgttttag ctcgtgaagt ttcaatggag gaagtggcag
     4681 agattcgtaa aaatacggat accgaaatcg aagcatttat ccatggtgcc atgtgtatct
     4741 cttattctgg tcgatgcaca ttgtccaacc atatgtcgat gcgggatgca aaccgtggcg
     4801 gatgttctca atcttgtcgc tggaagtatg gattgttcga tatgcctttt ggtcaagaac
     4861 gaaacagttt aactgataca ggcgaagtgg aagaagaatt ctcgatgagt gcagtggata
     4921 tgtcaatgat cgaacatatt ccagaattga ttgaaaacgg cgtagacagc ttcaagatcg
     4981 aaggaagaat gaaatcgatc cattatgtat ctacggtgtc aaatgtctat aaagcggctg
     5041 tagacagtta tatggaagat cctgaaaact atgtctgcaa gcaagaatgg atcgacgagt
     5101 tatggaaagt agcacaacga gaattagcta caggttttta ctacaataca ccaactgaaa
     5161 acgaacaatt attcggtgaa cgtcgtaaaa tcccagtata taaatttgtc ggtgaagtat
     5221 tatcttatga tgaggcaaca cagattgcta cgatacgtca gagaaacttg ttttctgtag
     5281 gagatgaaat cgaattttat ggtccaggat tcagtcattt cagtcaaacc gttgaagtta
     5341 tgcataatga agaaggcgaa tccattgacc gagcaccaaa tccaatgatg attttaacta
     5401 tgccagtcgc acagccggtg aaacctggag atatgatccg aaaacgtaaa taaggaaaaa
     5461 gctgatatat ccctgtttta agcatcttcg catacaaaat tcaagaaaag tgttacactt
     5521 gttttctgta gaaaaaggat cgttaaataa aggttttgat tgatctcttc aataaggagg
     5581 aacaaaatgc aaatatatga ttacatcgta attggtggcg gctctggcgg tattgcttca
     5641 gcaaacagag cagggatgca tggcgcaaaa gttttgctga ttgaagcgaa tgaattgggc
     5701 ggaacatgtg tcaacgtcgg ttgtgtacct aaaaaagtta tgtggcaagc aagtacgatt
     5761 ttggaaacaa ttgaacgaga tgcctcaagt tacggtatcc aagcagattt aaaaaatgtt
     5821 gatttcgccg gtctagtaga aagacgagaa aaatatattg attttctaca cggtgcttat
     5881 caaagaggat tagatagcaa tcatgtcgag gctataaaag gttacgctac ttttatcgat
     5941 agtcaaacga tcgaagtaaa cggtgaaacc tatcgtgctc ctaaaatctt gatcgctact
     6001 ggaggccgtg caagtaaaat gaccattcct ggtggagaat acgcaattga ttcaaatgga
     6061 ttttttgaat taacagagtt acctaagaga gcaatcgttt taggtgctgg atacattgcg
     6121 gctgaacttt caggtgtatt tcgtggttta ggtagtgaag tcatgtgggc atatcgaaaa
     6181 gaacgaccac ttagaacgtt tgataaaatg ctttcggaca atttgatcca aatgtaccaa
     6241 gaagctggaa taaaaactta cgcacatcat attgctaaag aaattactaa aaacaatgac
     6301 gaatatacgg tgctatttga aaatggtgaa accttaactg gtgattgtgt actttttgcc
     6361 ggtggacgtg taccgaatac cgaaaaatta ggtctggaaa atacaaatgt ggaattggac
     6421 aaaaaagggt ttatcaaagt agataaatac caaaatacaa cggatgaaca tatttttgct
     6481 gttggtgatg tgatcggtaa attagatctg actcctgttg caatcgcagc tggtagacgt
     6541 ttatcagaac gtttatttaa tggaaagaaa gattcttatc ttgactataa acttgttccg
     6601 acagtcgtct ttacgcatcc accaattgca acaattggtt tgacagaaga agaagcattg
     6661 gaaaaatatg gcgaaaatga attgaaagtt tatcgttcac gctttacccc aatgtacttt
     6721 gcattaaatg attatcgcca aaaatgcgaa atgaaattga tctgtgtcgg aaaagaagaa
     6781 agaatcgtcg gcttacatgc tattggtgtt ggtgtcgatg agatgctgca aggttttgcc
     6841 gtggctatca agatgggtgc aactaaagaa gactttgaca atacagtagc catccatcca
     6901 acaggagcgg aagaattcgt aacgatgcga taatttaata ttaacagttc caacagcttt
     6961 ttattgaaca aaccatgtaa aaccagaaaa tcagtatact gattttctgg ttttttgttt
     7021 tttagttatt tatgtaagat tgcttcgata attctcaaaa cgaaataagt agggaagttg
     7081 gagattttga ttatcttcgt aactgacgaa gagtaaatga atccatttgg tatgcactct
     7141 taagttcaaa atattggtat cgatgtttaa aagaaataga aatgtatcga agaacagatg
     7201 tttaaaaaag tgtgactttg tataaataaa ttagaagaag caaacgagga tgttaaaaga
     7261 ataccatata gttaccaaac caagaaagtt ctcgtcaagt tcgctggtac gaatcaaatc
     7321 aagtgaaaaa agggactgtg aaaaaaattg tgtcatagtt agtcactttt tccgaacaaa
     7381 tagtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
     7441 tttttccatt tgtttctcta atactcagga caaaacactg attttacaac ctctgataaa
     7501 cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtggaaagcc
     7561 attttcacaa aatttttctt aatactcgaa actaagcgac tttttcacaa cctcctgata
     7621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatataagga
     7681 gctattttta tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc
     7741 atgctgttaa atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt
     7801 gaataagacc atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt
     7861 gaattgatcc aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca
     7921 ttcatagata ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag
     7981 ctggaataag actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt
     8041 ttcaatatca cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa
     8101 caccaagggt aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaagc
     8161 aaattgtcca gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac
     8221 ataaacgacc atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa
     8281 aggcattcga gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat
     8341 aaatcccatc aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa
     8401 aacaaatgca atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg
     8461 gccaatgaca ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta
     8521 attattgata gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt
     8581 gtttagccaa tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg
     8641 aaatacacta gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat
     8701 ggttcgtact ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga
     8761 taatttgaag gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa
     8821 gatcgcttcc gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc
     8881 cattgaaaag actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt
     8941 gacacttgcg atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta
     9001 ttttttgcca tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt
     9061 ccgttcttta atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac
     9121 tgtccagtct tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa
     9181 gtcaacgatg gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat
     9241 gtaacctttg gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt
     9301 tttttctttg tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat
     9361 ttcttcttgg gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc
     9421 accaatcgcc atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac
     9481 taacgtgtag acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc
     9541 atcgatctca gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc
     9601 gaaggcaggt ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt
     9661 ccctaacaag aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact
     9721 ttcagtggtt gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc
     9781 cagtgtgatt aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa
     9841 ggtgagacga ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta
     9901 tgtgattatt tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca
     9961 gctgcattca ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct
    10021 gtaacaactt ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg
    10081 tcgttcagag tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca
    10141 ttatattctg tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat
    10201 tcagttactt tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag
    10261 ttcttttctt caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt
    10321 gtatctgtgt taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt
    10381 tcagatgagt gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag
    10441 aattggtcat tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg
    10501 tttttgttgt attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat
    10561 tttgattcag tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt
    10621 gaataatttt tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact
    10681 tttgcagatg attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa
    10741 gaagagtttg agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat
    10801 gctaatacgg caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc
    10861 atttctagtc cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata
    10921 agtcaactat ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt
    10981 tcgtgaaaaa acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga
    11041 acagaacatc tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc
    11101 atatattcat tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt
    11161 gtcgttgtag gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg
    11221 aaaaaagaag atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc
    11281 gagttgcatg aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag
    11341 catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat
    11401 aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt
    11461 ggaatgggcg gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc
    11521 ctttggtcat ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa
    11581 gctgcattgg aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt
    11641 tctggattta ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc
    11701 aaagattata aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg
    11761 atcttgaata tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac
    11821 aacgtggaat tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg
    11881 aaagacggac gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact
    11941 tctgacgctg ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa
    12001 tatatgcagg caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt
    12061 tatgaagaat tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga
    12121 cacacagccg cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa
    12181 ggaaactatc aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt
    12241 gataagatcc atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa
    12301 tacttctttg atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc
    12361 catatcaaag atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta
    12421 tggagtgtgc cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa
    12481 atcattggga atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc
    12541 actggaaaca gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt
    12601 gcttctgctg ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa
    12661 gcagcgactc aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct
    12721 gtgattgaga agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat
    12781 gatgtagcaa tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca
    12841 ttgattgctg gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg
    12901 ttctgcttat caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca
    12961 ttgatgaacg gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa
    13021 cgtacactag gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata
    13081 gataaagaac gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac
    13141 agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa
    13201 gacacaggtg tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc
    13261 ttcttcgaaa aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct
    13321 tcttatttct tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc
    13381 tgctgcttct cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct
    13441 tcgttcttat tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat
    13501 tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa
    13561 agtctgctca aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta
    13621 ccagtcatga tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg
    13681 cgtgtggatg gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca
    13741 tatacttaca aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta
    13801 cggataaaag aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa
    13861 aatggcgaaa ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat
    13921 ggaggtgacg aagacagtct gatttattag accttcacac accatgagta aattacaaaa
    13981 aatgatttat atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat
    14041 gatcccttat cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac
    14101 gatcgttgcc atttttacca tgaaaaaacg agacagcttc ttactacttt ggctacgact
    14161 attcctgact actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc
    14221 tttgcttagt tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat
    14281 catcggaatt agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc
    14341 tttcattgca caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat
    14401 tttatcaggg attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact
    14461 tcaacgattc caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac
    14521 caaaaaataa tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact
    14581 atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc
    14641 tgaaaaacaa acctgtcgaa caagcaatca aagaaacgat ccatgccggc ggaaagctgt
    14701 tgcgtcctgc ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag
    14761 ccatcgcttt ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata
    14821 tcgttgatga agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg
    14881 tcgtttctgt ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt
    14941 actccacttc cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg
    15001 gcgaattagg gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg
    15061 aaaatatttc aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg
    15121 aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga
    15181 tggcctttca gattatcgat gatattttag attatacaaa agagagccag gaaatcggca
    15241 aacctgtact agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc
    15301 aaaaaaacaa accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag
    15361 atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt
    15421 tagcggcctc ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc
    15481 ttcgaacaaa agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa
    15541 tagggaaagg ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca
    15601 aattgaattg tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta
    15661 gcgacgttgc tcttttttat cttgaatatt tttttacctt ttcgatttct tataactcat
    15721 aaaagtgatt cttaaaccga ttacaagtaa tataacgcct gcgagtgtta tccaattatt
    15781 cgattgactt ccagttttag gaagtgctct ttttgatttg tttccattgt cattgcttgt
    15841 tttattagtt aagttaaagt tcgtcctgtt atccgaactt gtttttgaga aggagacact
    15901 tgcatgacgt gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg aagttgatga
    15961 atctgagcta tcacttgtgg tagaggatgt atctgtactg tcacttgaag ctgacgtgtc
    16021 cgtgctatca cttgtagtgg tagatgaatc tgagctatca cttgtggtag aggtagtatc
    16081 tgtactgtca cttgaagctg acgtgtccgt gctatcactt gttgtggtag atgtatccgt
    16141 actgtcgctt gaagctgacg tgtccgtgct
//
LOCUS       DAGCXG010000052        15909 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGCXG010000052 DAGCXG010000000
VERSION     DAGCXG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.055"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..965
                     /locus_tag="MB022_001701"
     CDS             <1..965
                     /locus_tag="MB022_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466699.1"
                     /translation="LLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEM
                     NKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDT
                     PDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAF
                     GKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIK
                     HEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYD
                     QTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(991..1431)
                     /locus_tag="MB022_001702"
     CDS             complement(991..1431)
                     /locus_tag="MB022_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4466700.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1591..2097
                     /locus_tag="MB022_001703"
     CDS             1591..2097
                     /locus_tag="MB022_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4466701.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2265..4178
                     /locus_tag="MB022_001704"
     CDS             2265..4178
                     /locus_tag="MB022_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBT4466702.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4183..5184
                     /locus_tag="MB022_001705"
     CDS             4183..5184
                     /locus_tag="MB022_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4466703.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5386..5592)
                     /locus_tag="MB022_001706"
     CDS             complement(5386..5592)
                     /locus_tag="MB022_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBT4466704.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(6238..7086)
                     /locus_tag="MB022_001707"
     CDS             complement(6238..7086)
                     /locus_tag="MB022_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4466705.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            7085..8152
                     /gene="gnd"
                     /locus_tag="MB022_001708"
     CDS             7085..8152
                     /gene="gnd"
                     /locus_tag="MB022_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBT4466706.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            8154..9155
                     /locus_tag="MB022_001709"
     CDS             8154..9155
                     /locus_tag="MB022_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBT4466707.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            9152..9436
                     /locus_tag="MB022_001710"
     CDS             9152..9436
                     /locus_tag="MB022_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBT4466708.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            9469..10266
                     /locus_tag="MB022_001711"
     CDS             9469..10266
                     /locus_tag="MB022_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4466709.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            10280..11113
                     /locus_tag="MB022_001712"
     CDS             10280..11113
                     /locus_tag="MB022_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4466710.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            11131..11988
                     /locus_tag="MB022_001713"
     CDS             11131..11988
                     /locus_tag="MB022_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4466711.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            12010..12519
                     /locus_tag="MB022_001714"
     CDS             12010..12519
                     /locus_tag="MB022_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4466712.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            12556..12957
                     /locus_tag="MB022_001715"
     CDS             12556..12957
                     /locus_tag="MB022_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466713.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            13069..13422
                     /locus_tag="MB022_001716"
     CDS             13069..13422
                     /locus_tag="MB022_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466714.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            13456..13899
                     /locus_tag="MB022_001717"
     CDS             13456..13899
                     /locus_tag="MB022_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBT4466715.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            13916..15001
                     /gene="uxuA"
                     /locus_tag="MB022_001718"
     CDS             13916..15001
                     /gene="uxuA"
                     /locus_tag="MB022_001718"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4466716.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            14994..15707
                     /locus_tag="MB022_001719"
     CDS             14994..15707
                     /locus_tag="MB022_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4466717.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         5526 a         2483 c         3374 g         4526 t
ORIGIN      
        1 aactattaga aaaatttgcg aaacatgcgc catatacgcg tagtatagat agaaagaaat
       61 ggatcagcct actaagaatt tcgttgcaac gaatgaaaca aggccactcg attacgtatg
      121 tcccagaaga gatgaacaaa tttgtgcatc cattgataac aaaagaagag tttacgcatt
      181 acatactaga gccttttttt gaagcaaact tctggtcgat gaatcacatc actccaacag
      241 agcaagctta cctttatttt atgtttggtg taatgaacac ctatttggaa gaagatttgt
      301 tggatactcc agataaagta caagaaagtt ggggctggat tgaatcgtct attgtagaaa
      361 attttgaaaa aaacttcaag atttgtttta caacttcaga aaaaaattat ctatatgtca
      421 atttggcaat gatccatttg tattcaaggg tttttgggac aaaaaagaag gtggatgctt
      481 ttggaaaatc agcagaagac agagattttc agcatatatt tccagtgatg tatccaatca
      541 tgcaacagtt ttttagtggg gtagctcgca gaattccaga gcttaatact tattatgaaa
      601 agaacagacg gttgattttt caatactgta tgttagtcag ggatattttt ataaaacacg
      661 aacaacctgt catattctat attcaatcaa agtatggtaa aacacaggaa ttatgggcaa
      721 agaaaaggat attatctatg actgaacgcc caataaaata tagtgcatcc gctgatcagt
      781 tacctgatat tgtcattagc gattatccta tcgacaaaaa ggcatatgat caaacgaata
      841 cacagatatt ttactggaat ggacaaccta caaaaaatga ttgggcgcgt ctgaaagctg
      901 aaatcataaa aatcaggaat gagaaaaata gatccatatt ggaaaaattg gatttcgtta
      961 attaagaaaa gcagattgtt tatgccaaaa ttaagacatt gcaaaaagat tattttcttt
     1021 aaaatcgaca agatgaccag aaagcaagag ttcgttgagc tcttgctttc ttttagtttt
     1081 agaaaggaac atcctgattt tatcttcgct aaatccagtc aaaagttttg cagattggtc
     1141 gtagataatc ggttttttca ataaagaagg ggatgtacga atctcaatat atagttcact
     1201 caaactgagt tcatctattc tgtgaacaag atttttataa ctaatagaat gagtagagag
     1261 catttcggtc acgccgttgt ttgtcaattt tagtaagtgg ataaattcat ttttggttag
     1321 tggttgacga attaagttct gttcgataaa cggtatatca tggacgataa gccaacgttt
     1381 tgcgcgtctt gaagacgagc agccagaacc tgtatacaat ttaatcatca tacattcctc
     1441 caatagctaa gtatttatga aataataaca tgaagaaata gtcgaaaagt gccatttaga
     1501 ggtgtaaaat aagaaaatgt tgtcacgtat aatggaacta gctagataag aaatactatt
     1561 aaatcaagaa aaaagaaaga aggcacaagt atgtcaaaca cagccgacgc aatagtagtg
     1621 atcgatttac aaaaaggggt atgttttgat tcttcgccga tacacgaatt agaaacagtg
     1681 acagcattag tgaataaaag aatcaaaaca tacgaaacga ttgggaaacc agttattttc
     1741 gttcaacacg aagatgctca attagtgcca caaacaaaac catgggagct attgtcggaa
     1801 ttatctattc ctgagcacag ttattttgtg agaaaaaccc atgcgaattc attttataag
     1861 actaatttga gtgttttatt aactgaattg gactgtcatt cgatcgaatt atgtggggca
     1921 caagcagagt attgtataga tacgactgtg aagtttgctc atggattagg ataccagctt
     1981 atgatgtgta gaggagcaac gacaacttat gataatcctt ttatgaaagc aaaggaaaca
     2041 atcgcttttt acgagaaaat ttgggataaa cgatttctga catttttgga gcaataagtt
     2101 gtttcttcgt ttaaaaaaag cagaatggat actggtttat ccattctgct ttattacgta
     2161 aaatgagaaa ataatcctgt tctattttta tgggataaac catttatttt ttgtataaaa
     2221 aatgtttgcg tttacatata attctcctag aaacataaaa aggagtgaat agcttgagaa
     2281 caaaacaagt attaaaggga gcgttggtca tcttatttgc tgtaagtaca agcgtcccgc
     2341 aatggacagt atatggagag agcatttctt ccgaatcgga atctcgggag gtagaaacca
     2401 cagaaactac tgatgctgga aatgaaacga aatcgagtac ttcacaaaca gaaacaaata
     2461 ctgaaacaac ggttgagaca tctgttacta gcacagatca agaaacgacc gctaccacag
     2521 ataaccaaca aacagaagaa gcaacagcta caaaaatgtc aaacgacgaa tcctcttctg
     2581 ctaaagtaag tgacaaagag aatgggacag aaaatgatga tatcggtaaa ggtgaaaatt
     2641 tcaatggtaa agaatatttg aattacttcg gtaaacagat gacagaaata cttaatggac
     2701 caacagaaga catcaagtca aaattaatag agttattagt cactgagctt gctgttaatg
     2761 attatggtgt agataaagag attatcaaac atctgattgc tacttatcta ttgtacgata
     2821 tcgcttttgc aaaaaacagt gtgttgatgt cgatgcttgg aggtacagat gatgggacaa
     2881 cgcctacaga aaacacaggc ttctatccaa atacaaaaca acttcatcaa actgtactaa
     2941 acaaacaaga tggcaaaatg atggatgtat atgcatatta tgtcgatcaa ggttcagata
     3001 aaacagtcat gatccatggc ggatttcgag ggaattggaa taacgggatc gttacagaag
     3061 aatacaatga tttttataag gccgggtata atttattatt tgttgattcc agagcgactg
     3121 gtaatagcgg aggcgattat gtgacctatg gacaatatga gtcagatgac gtcctttact
     3181 ggataaatca agaagttcga gagcggccat ctcaaaagat tttactttat ggtggttcga
     3241 tgggagcagc tacgatgatg tctgtattag cgaaagatat tcctgtcaat gtcaaaggaa
     3301 ttattgaaaa ttgcggattt gcatcaattg atgaacaact acgttttacg tacagccaga
     3361 cagtagcccc cgcactaccg gatgccataa aaaatcagtt agacatcatt ggggatcaag
     3421 agcatgaaga tttatttatg ggccttttga aacaatacta ttttgatcag gagatgcatt
     3481 tggatacaaa agctgctcta cctacgattg gaatgtcaga ttcattgcct aaattgatta
     3541 tccatggaac agcagatgat gtagtacctg tgtctaacgc gcaaaaatta tatgagttat
     3601 caggagggta taaagatctt ttattagtag aaggagcagg gcatggaaag gcacaggaag
     3661 ttgaccatgc tgcctacacg aaacatgtaa cggacttttt gaaagtagtg ttccatgatc
     3721 agataaacgt aaagtatgtg gatgaaaaca ataaaagtct actcaaagac caggacgaaa
     3781 tccagcttta cggagcttat ggagaaaatt atatgacaga gcaaaagaca ttcgaaggat
     3841 acgaattagc aaatgttgaa ggaccgactg aagggatttt taatgagacg ataccaacta
     3901 ttattttcta ttacaaaaaa atcccagtag ctccaccaaa aaagcaagat ccgacagaga
     3961 atgcagacaa taagggaaag aatgaacctc gagaggatca atcaagtgat gatacgcaac
     4021 cgattcagat gacagtgaaa gtacaaaaaa agacagatgc aaaaaaaaga atgttgccga
     4081 aaactggaga aaagaaacat tcgcttctca tttattcagg tggattttta tctgttagca
     4141 cgattatcac atggatttgg cgaagaaaga gagggtaaat gaatgaaatg gttcaagcga
     4201 atagtgattg cagtaccagt tatcatcgta gttattttag tcactgtatt tttgattaat
     4261 caaatcacac caaagccgat tagcttgctt gttagaaaac aatttgacac aagtgaaaaa
     4321 gaacaagtat atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat cgatataaaa
     4381 aaagaaatat cctatccttc aaagtacaaa aataataata tggatatcta cacaccgaaa
     4441 gtggaaaaga aaaaattacc gattcttttc tggatgcatg gaggcgcata tgtaggaggg
     4501 gataaaaatg attgtcgtga ttatttggaa tatctatgtt cagacacgca gcaaatcatc
     4561 gtaaatatag attatgaacg atcaccggaa gcaaaacatc ctacaccagt tatccaattg
     4621 aacgaagcca ttcaagcagc taaaaaagag ataaaagatc aagcagattg gtcgaatatt
     4681 tcgattggtg gaaattcggc aggtgcacaa attagtggag aatatttgtt agcacttcaa
     4741 aatgaagaaa ttaaaaaagc tgatcatctt gatccgactt tggagaacaa gcagattacg
     4801 aagtttattt ctttgtctgg tctgttggat ccttctgcac ttactcaagt aagtgataaa
     4861 atctctagct ttctttatga aaaatgtgga tgggcttact ttgataataa ggactttgaa
     4921 caatcagaga aggtcaaaag tttagccttg gtcagacatg cggatcgttg gacgcaaaac
     4981 acattcttaa cggatggaaa tacaaatacg tttacgaaac aaatggaaga agtaacttct
     5041 gtatttgaga aagcgggggt gaaagtcaca aaagtagatt atgcggagaa aaatgctgtt
     5101 ttgaaccatg aatatcagtt tgatttcagc aataaacaag cgcaagaaac atatcaaaag
     5161 ttagtaatgt ttttcaagga gtaaagtctt atgatgcaaa agaggttgta atctgtcact
     5221 tagctccaag tcacaaggag caatttcaat aaacaagctt ttcatggttt atgaatattg
     5281 taacttgtga ctgaggagtt agagatggca aggggcacct tttattcgaa atttgggaat
     5341 atcacacgat tattgtcacg atctctttta cgtaaatttt ataagtcaga tttgttgttt
     5401 tgatcgttta aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg acaaaaagcc
     5461 gagtggtaag caaaagaatg ctggttccac taatattccg ttgttattga tataaggctg
     5521 ggaacaaaga tgaatagcaa ataaaatcaa tgcactagcg aaaaaaagca aagaaatatg
     5581 gatagctttc ataagcagca cctccttttt tataattgta tgataaatag attaagaatc
     5641 tataaatgtg acaagtttgt taggatgtga ataaaatgat catctagcga acttgaaaca
     5701 aagatggcta atgataagca attaatgatt cagcaatcag ataggtacaa agtttaccat
     5761 catacgtcta gaccaataat agaaaatatg ggaatataat agaaaagatt taggataccc
     5821 ccaaaagaat ttccccaaaa attcgatgta tcctaaactt ttttatttta cataacaatc
     5881 atatcaactg tgaaataaaa ttacaaatga taacctaaaa tgaggccaaa agccagtgtt
     5941 atcaaggaaa tacttaattc accaaaattt tttttaattt tttagaaaat aagaaacaaa
     6001 aataataaaa aaaagaaaag ataaattttt tactactgta tactaacttt gttatcgcat
     6061 ctaacaagta actcgaataa taccaataca accttctcca aagatgcgat gacatacgtt
     6121 ctaacccacc gactattgat gttctgtacc acccttgcag aaccaaaaaa gccactacca
     6181 aatatttggt agtggctttt tttgttttat ggatttttcg ctttttctgt tatggtgcta
     6241 ttctttgatt tcagaaatag tatgtcgaac tttagcgata gaacgacgtg attctttttc
     6301 gttgtaaaac atcaaaatca catatagaga gtccaaaatg cttagttgag aaatacggga
     6361 agctaaagct tcagatcgga attctgtttc ttcagaaata gaaataaaaa cgacatcgcc
     6421 aagtttagca agggaagaat tagcttggct agtaataacg attgtttttg ctccagtttt
     6481 tcttacggtt tcagcaatat ggattgtttc ttttgattgc cctgtgtgag aaatcaaaac
     6541 agcacagtct ttttccgtca ataaagaagc ttccatcaat tgaatatggt aatccgtgct
     6601 gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg ctacaatctc
     6661 tgaaccgcca acaccgaaaa aatagacacg tttagattca ttgataatag tagccgcttt
     6721 ttttagatcc ttttgttgta acaacttttt tgtgtcattt aaagttgaaa tactagactc
     6781 gaacaccttt tgcgccatag tgagctcagt atctgttttc tgaatatttt cgtggataga
     6841 aatagcagat agatcatttt cttggatcaa catggccatt ttaaaatctt taaatccatt
     6901 ataaccaagt ttttttgtaa attgaaaaaa agtagagtca gcaatgccca gttcacttgc
     6961 taaattgcta ataggactat gacttacttt gcttggattt tctaaaatat aatcggcgat
     7021 agcctgttct tttgggctga agtcgttgta cattgttttg atttgtagtc ttatggagac
     7081 gctcatgaga attcctcatt ttcataataa gattatcatt ttttccgttc tcattaatgt
     7141 taaagataca aggagagtag gaaaaaatca aacaaaaaat atttttgtaa aatctattta
     7201 caaaaaaatt tttttgtata atgagggtat aagaaatgga ggcgctatca tggaaattgg
     7261 tgtaattggt ttgggaaaaa tgggattgaa tttagcttta aacttaaaag agcaccatca
     7321 caaagtacaa ggactcgaca tttcaagtac agctgttagt caggcaagag aaaatgacat
     7381 tcctgcttat caagagatct ctgaatttct agctactttt gagcagaaaa aaatcatctg
     7441 gttaatgctt cctgctggag agataaccga aaacatgtta agaaaacttt ttccaaaact
     7501 cggaccagga gacattatta ttgaaggcgg aaactcttat tataaagatt caatccgtcg
     7561 agcagaagag tttcagaaaa atgaaatcgg ttacttggat tgtgggactt ctggaggaat
     7621 cgaaggagca cgacatcaag cctgcttaat gattggagga gataaagaaa cttttctatc
     7681 cgtggaacaa ttattcaaag atgtagcaat tgaaagagga tatctgtatg ctggtgcatc
     7741 cggaagcgga cacttcttga aaatgatcca taatggaatc gaatatggaa tgatgcaagc
     7801 aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat gcttttgact tggaacaagt
     7861 cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa tcatggaaga
     7921 tcagttaaac gaacatccca atttaagtaa ttatcgggga atcgtttctg catcaggtga
     7981 agcaaaatgg acggttgaaa cagcgctaga aatggctgtt ccagttccag taattgcaac
     8041 tagtttgttc atgaggaatc tttcacagga agaagatagt ttttcagcaa aagtagtttc
     8101 agcactaaga aatgggtttg gcggacatga aatagttgaa aagggtgagt aaaatgccag
     8161 attatcatat agcaatcgtt aattccagca gtttcggaag agtctttcca caccacttgg
     8221 aaagattaaa gtcagttgga gacgttcatt tctttactgt tgatcaagag attccaggaa
     8281 aagaactagc ggagttactt aaaggattca atatcattat tgcaagtgtt acgccatttt
     8341 ttcgacgaga tttttttgaa aacaaagatg aactgctatt gatttcccga catggtattg
     8401 gttacaacaa tattgattta aaagcagcaa aagaacacca gacaatcgta tctatcatac
     8461 ctgctttagt tgaacgaaat gctgtagcag aaaataatat aactaatttg ttgtccatgc
     8521 ttcgacgcac gacacaagca caagcaagag tcaaggacga tcgatgggaa gatcgggcgg
     8581 agttcgtcgg taggacgctg tttaacaaaa cagttggtgt gattggtgca ggaaataccg
     8641 gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta gcttatgatc
     8701 cttacaaatc agatttatac atcgaaagct ttggtgcaaa aaaagtagaa cttcaagaat
     8761 tactggaaca atcagatatc atttgtttat gtgcgaattt gacagaagag aactatcaca
     8821 tgatttctga taaagaagtc gatcagatga aagaaaatgt ttatctatct aatagtgcaa
     8881 gaggcgctct agttgatgaa gaagcgatga ttaatggtct gaaaagcgga aagatcgcag
     8941 gatatgccac agatgtttta gaagaagaac cgggacgtca agatcatccg tatttagcgt
     9001 ttgaacaagt agtgattacg ccacatatat cggcctacac aagggaatgt ttagaagcaa
     9061 tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca gcaccttctt cctgaacgtt
     9121 ctgtccagac aaagagcaac tatataaaag gatgatgaag aaaatggtga acggtttaaa
     9181 agtgaaaaca ggccctcaat tttatttata tgaagaaggt ggtatttcaa aagtttctga
     9241 tctcttgaaa tcatacggcg cgaaaagagt actggtcacc cacggaacgg tatcatggga
     9301 aaaggcgcta cctaaattag tatttttgaa tgacgaaacg attcaatttt tttatcatcg
     9361 ttattctgga gaatgtagtt atgctgaagc cagacgaata gctacaatca tcaaaaaaat
     9421 gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga ccgatctagt gggttatagt
     9481 gccaatcttg caagaatcga tttcggagtc atccctactt tagctagtaa ttgtgctcca
     9541 tggacacctc ttgctgtcat gtataaggag aatggacaag cagaagggaa aacagaacac
     9601 ttttacagac aagctgcttt ttttataaca gatccccaat tagtgattga ttcccctgtt
     9661 ccatatttca ttgccgggtt agcagataca ctagcaaaat ggtatgaatc tgagctcatc
     9721 ttagaacaag attttttgca agatgaatca tttttgaagc tagcacaaga tactgcgaag
     9781 atctgcaaag aagagatttt gaatcattct gctaaagcaa tccaagatat ggcacaaaga
     9841 aaactgacat cggagttcaa acagctttca gagattatct ttgctgttgc tggtttagtt
     9901 ggaggattag gagataaata cgcaagaaat gtggcagcac atgctatgca tgacgctatc
     9961 agtaaatata ttccggaaag ccatcgtttt ctacatggag aaaaagtagc gtatgggatt
    10021 ttctatcaac tggcactaga aggtcgctgg gaaaaaatag acgagttata tcctttgtat
    10081 cgggaattag aactgccggt ttctctagaa caaatgggat tatttccgaa agataaacag
    10141 ataatagata aaatggctga atttattgat tcgaaagaga aagtgcattt gattcctata
    10201 gaggtaacac agaaatcact gatacagtca atgatggaac tggaaggtta tatcagaaag
    10261 atctgaagga ggaggacaca tggaaacatt aagtgtagta caaagtttgt tgattgcttt
    10321 atgggtagcc gcggttatgt cccgctggtt aggtggagga gccacgttga ccttacgttt
    10381 ttctcctttg atgactggat tagtcgtcgg gttgatcatg ggggaagttg cacaagcgat
    10441 gattgttaca gccgctttgc aaatgattta tatgggggtt ttttctccag gaggatcaat
    10501 gccagctgaa ccatctattg cagcagctat cgcagtacca gtcgcattat taggggatct
    10561 gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa gttacttgta
    10621 tcagtttcgt tttttcatca atactttttt aggtaaatat acagacaaag ctgtagaaga
    10681 tttgaataca aaagctatgg cccgatcaat tattctttat ccaacggttg cctcattttt
    10741 attattcgtt ccgcttgttt tcattgcttt atatttaggg gcgccattga tcgctgatgt
    10801 aatcaaagct ttagagggaa cagtcgttat ccacattctt gaagtagtag gtggagggtt
    10861 agcggcaatc ggtatcgcga caactgtata cgttatcggt cgaaaagatt atctggtctt
    10921 tttcttcctt gcttatttta tgagtgttgt tttgaaatct ttagatatta cgatggttac
    10981 ttatgccatt tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa aagggaaaca
    11041 agtacagcct gccacaagca ataacaattc agcatctttt gatgacgaag atgactatga
    11101 tgacggattt taatttagga ggaatgggaa atggctgaaa taatggataa tcaaacaggt
    11161 gctcctgaag aaatcacgaa aaaagatgta acaaaagcct atctgagatg gcattttgca
    11221 aacgaaattc ctcactcttt tgaacgttac ttggcgccat cactactata cggaatgatg
    11281 ccaatcttga aaaaacttta taaagatgaa gactctttaa aagcagcata taaacggcag
    11341 ttactgttct tcaacactca attaagctgg ggtggcggtg tgattactgg cctaatgtct
    11401 tctatggaac agcaaagagc agaagaagag tatgaagaaa aagagatctt gatgcaggat
    11461 gacttgatgt ataacacgaa agcgggattg atgggggcac tagcaggaat cggagatgca
    11521 atcgattcag gaaccgtcca atatattttt atcgcaatcg cggtaccttg ggcacaacag
    11581 ggaagtcctt taggcgcact ttttcctttt atctgctttg ggttgtatca ggttttacta
    11641 ggggtatttt tcgcaagaca agctttcact atgggccgta atgctacagg cctaaagcaa
    11701 aacactggtg ttcagacagc gattgaaatg ctatcagttt tagggttgtt tatgatgggg
    11761 gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga aatttgcgat atccggacgt
    11821 gagtttgtta tccaggaaat tcttgatcaa atcgtgccag ggatattacc tttagctgtc
    11881 gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga aagtcacaca agcattatta
    11941 tggctgactg gaatattaat tgtgctagca ggaatcggta ttttataaaa agaagggaac
    12001 gtgaataaga tgggtgtagt caatttagcg cgtgtggatg agcgtttgat ccatgggcaa
    12061 gtaatggtaa cgttatctca aaaaagtgga gtgaattcca tttttgttgt agacgaagtt
    12121 gtagcaaaag ataaatttat gagagatctt tacaaaaatg caggaagccg tacaggacaa
    12181 aaaacaattg tcattacacc agagaaagct aaattctatt gggatgaata tcaatttaaa
    12241 gaatacaatt gtattttgat cgcaaaaaca gtccaaacga tttacgactt agtaaaacac
    12301 ggaatcccca tggaagaatt gaatattgga gggattgctc aaaaaaatcc tgatacagat
    12361 cttttagtga cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg
    12421 aatgaacaat atggcgtaaa gaatatttat tttcaagcca ctccatctgc acctaaaaca
    12481 ggattaaaag atgtattgaa caagtttgat ttgtcataaa caaatgctta gaaaaaagat
    12541 aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc
    12601 tatatttacc gtaagcgccg tacaaaaaag agtaaacagc gttttctttc agcattagta
    12661 tcagatattt acagtatgag aacagatgtg acagtcattg cttacgatac gctagcctac
    12721 cgatcaaaaa atatttatgt tggagatatc gaaaaagcag agaaagttat ctgtacttat
    12781 tacgatacac ccgttcatgc attaggaagt tatttcatgt ttgattggaa agaccaaagg
    12841 aaaaaaacaa tctactctat attattgtcc ttcattttgt tgttcagttt aggttggtgg
    12901 ggaatgatga tatataacaa aaatcctcat cacgtattcg atctgctatc cgtataaaca
    12961 ggtatcactg tactagcatt tgggagctac tttttcttat tgggaaaagc agctcgcgga
    13021 tggcctagca gacaaacttt tattcgaaat acttcatcca tattaacgat gctagaaatg
    13081 atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga
    13141 gaaaagggct tagacagcgt gagatcgggt atgaaaaaag aggctattct tttttatcta
    13201 gactctgttg gggcagatac accgctacag ttttcgggaa attatttttc aaataaagaa
    13261 cagtggctga aacaagttga taagttgaaa gaaaaaaacg tcaattatat ttttagtgca
    13321 agaaaaaagc aagcgcagtt cttctatttg accaaaacag atttacgagg gaaaacattt
    13381 aattggcaaa atgccaatca gattattgct ctttttcgat aagtaaaaag tgcaaaacaa
    13441 ggagtgagaa gactaatgtt ggaaatcatg atcgttacac atggtaagtt gagcgaagga
    13501 ctgaaagatg cggcggaagt aattgtcggg acatcgagcc atattgaaac aatgtgctta
    13561 gagtctgggg aggacatcca aacattcgga gagaaggtca aagaaaaatt aactatgatc
    13621 aacacagcag aacccatgct agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa
    13681 gcgctcttgg cagtcaatga actgactgaa gaaaagaaag cagccactta tgtcataggg
    13741 ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc agatgcttca cacaccgata
    13801 gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata atttaactgt ttggcatgct
    13861 tcagcggaag aaaaaaaatt agatgaggac gatttttaga aggaaggctg aaaagatgaa
    13921 gtggggattt agatggtatg gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca
    13981 aataccggga ataaatggga taattggaac tttgctgact aaaatgcctg gagatgtttg
    14041 ggagatttca gaaattgagg agttgaaagc atctgttgag agccaaggac tggaactgct
    14101 tggaattgaa agtgtagcga tccacgatgc aatcaaggca gggacagatg aacgagatta
    14161 ctatattgat aactatatac gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat
    14221 ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact gatttgttta atgaaaacaa
    14281 cgatggaagc tattctcttt tatacgatca gaaaatcgtc gatcagatgg agccaggaga
    14341 aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga
    14401 acggttgaaa aaattcgctc agctggaaaa actgtatgaa ggaatgaccg aagaagaatt
    14461 atttgaaaat ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt
    14521 aaaaatggct atccatccag atgatccgcc atgggaactt tttggttttc ctagagtgac
    14581 gaaaaatctt ggcgacttga agaaaattct aaatgcagta gattctccat ataatggact
    14641 aacgctgtgc acaggatctt taggggcaaa tccggaaaac gatttagttg aaatcatcca
    14701 tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac gtggagtttt taggagaaag
    14761 aaagttcaaa gaaacggcac atccctctac agaaggctcc cttgatatgt atcatattat
    14821 gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt
    14881 gtggggtgaa gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg gactcactta
    14941 catgcaaggt ctatatgagg cgataagtaa ggaaaaacca ctgggagagc attatgaata
    15001 aaatcaagct cttcatcttt gatatggatg gccttttatt agaaactgga cgtttagctt
    15061 atcgtgctta tgtaaaatca gcacaaaagt atgattatga aatgcgtaag gaagtctatt
    15121 atttactaac tggccaaaca gaaatggcga taagagaaca aatgggcatt ttgtacggag
    15181 aagatgttcc atatatcaaa tggcgagaag caatcaatca atataaagaa aaaatcgtga
    15241 aagaagataa gagagtatat acgaaaaaag gagcagaaga aattttatct tttgcaaaag
    15301 aaagaggaat ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt
    15361 taaaaatgga aaatctatat gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa
    15421 aaggaaagcc ggaaccagag atctttttga aagcttgttc aaaaatgaat attcctccaa
    15481 gtgaagcact tgtatttgaa gactcgattg cagggatcga agcagcaagg cgagcgggca
    15541 ttttgtcttt tctcatcaaa gatcatttga tagggttgcc cgatcataat gggaagcata
    15601 agttgaaagt agatgtaagt ttatatgaat ccaataaagc agactatgaa ttttatgacc
    15661 tgcatcaagc aaaacgattt ttagaaagta aagggttgta tctctaataa agaggactat
    15721 tttctagtcc tctttattta ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa
    15781 ttttttattt tgaagtatct accttatttg tagtgaacaa tttagaacaa acacaaaacg
    15841 catctactgt tatctaccag tagatgcgtt taaagagaag aacgagaatc gtccatatga
    15901 gggtttcca
//
LOCUS       DAGCXG010000053        15894 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGCXG010000053 DAGCXG010000000
VERSION     DAGCXG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.056"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            71..754
                     /locus_tag="MB022_001720"
     CDS             71..754
                     /locus_tag="MB022_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBT4466718.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            756..1592
                     /locus_tag="MB022_001721"
     CDS             756..1592
                     /locus_tag="MB022_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4466719.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYFNLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1603..3357
                     /locus_tag="MB022_001722"
     CDS             1603..3357
                     /locus_tag="MB022_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466720.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3633..3974)
                     /locus_tag="MB022_001723"
     CDS             complement(3633..3974)
                     /locus_tag="MB022_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4466721.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4042..6213)
                     /locus_tag="MB022_001724"
     CDS             complement(4042..6213)
                     /locus_tag="MB022_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4466722.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6382..7281
                     /locus_tag="MB022_001725"
     CDS             6382..7281
                     /locus_tag="MB022_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4466723.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7352..8209)
                     /locus_tag="MB022_001726"
     CDS             complement(7352..8209)
                     /locus_tag="MB022_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4466724.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8194..10896)
                     /locus_tag="MB022_001727"
     CDS             complement(8194..10896)
                     /locus_tag="MB022_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBT4466725.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10909..12198)
                     /locus_tag="MB022_001728"
     CDS             complement(10909..12198)
                     /locus_tag="MB022_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBT4466726.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12348..13394
                     /locus_tag="MB022_001729"
     CDS             12348..13394
                     /locus_tag="MB022_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4466727.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13396..15549
                     /locus_tag="MB022_001730"
     CDS             13396..15549
                     /locus_tag="MB022_001730"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBT4466728.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4519 a         3312 c         2931 g         5132 t
ORIGIN      
        1 aatttgctcc gtcttcttct cttgctgttc tgcttcattg cctataaacc tttatgaaag
       61 aagagataac atgcctacta tccatattgc cggtgattct actgcggcag aaaaagatga
      121 aaacaagcgt cccgagacag gttggggaga aaaaatctct gcttattttt cacccgagat
      181 ccagatcaat aaccaagcaa agaatggacg cagcaccaaa tcatttatcc aggaaggaag
      241 gttagcccgt ctagcttcac agtttcagcc gaatgatttc ttatttgtac aatttggaca
      301 taacgaccag aaaatccaag aagaaagagg tacgcttccc ttcggtagct atttaaaaaa
      361 tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc
      421 tgtgacgcga cgagattact tagaaaacgg aacattgaat ccagatatac ttggagacta
      481 tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt cctcttttag atgtgttctc
      541 aagatcacaa gaattgttcc agaccttctc aaaagaagag acaaaaaaat tctacctgca
      601 tcttatgcct gatacttgta aaaattaccc agaaggacta aaagacaata cccactttag
      661 tccagaaggc gctgataaag ttgcccgaat aattgttgaa ttgataaaag aatcccgtct
      721 gcctcttgct aattatttac aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc
      781 gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc
      841 acttgtccca tatccaattt gcaccacaaa aagcatttcc tgaacaactt aaattaactg
      901 acatgacact tgggagtgct gcctattttg gtgaatactt ccaaaaaaac aatatcgaac
      961 tttccatttt aggatgctat ttcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag
     1021 cattggcaac tttcaaacag caaattgctc tgtgctctgc ctatcaagcc aagatgattg
     1081 ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga aaattttact gaagaagctt
     1141 atcagatcgc cagaaattct atcattgaat tagtcgctca tgcagaaaat tttggcgtaa
     1201 ctgttgctgt tgaagcaggg atcaatcatc caatctataa ctatcagtta gctaaacgtt
     1261 taatcgatga agtagcttca ccaaatttaa aaatcatttt agattgtgcg aatttgatgc
     1321 ataaagaaaa tcatgctgat caggaaaaag tgatccgagg tgccttggac aatttacatg
     1381 gttatattac agcgcttcat ctcaaagact atatcatgga aaatggaaag atccgcatgg
     1441 tacctgttgg caagggctgt ttagatttcc gcccggtact gcattatatc aaatatgagc
     1501 gtcctctaat gtacgccact cttgaagcta ctcctgagat ttatgttcct gaagcaatag
     1561 aacacttgca gtttatttac caagattgtt aaggagaaga ttatggagga acgaatacag
     1621 aaaagttatc aaaaatatca tgcaaatgct tttcagacat tgctgcatct ttatcaggga
     1681 aattattggc gttttgccat gtcaggactc ttttttatta tcaaacatct tccatcttgg
     1741 ttaatgccga tcgctattgc caatgtaatc aatgcagctt catcgaaaga ttcttctcag
     1801 ttgcaggcca tttatttcaa cgcttttttg atggcttgtc tgattggtca aaatatattg
     1861 acgaattatt ttcatgtaaa ataccatagc acttcgatcc gtcaagtaga agctggacta
     1921 agagcgaccc ttatcaagaa aatccaagaa ctatctattc cttatcaaaa agaattgcaa
     1981 tctggacgaa tccagtcaaa gatcatccgt gatgtagaag ctatccaaac gttgtcctct
     2041 caagtatttg tcagcggatt aaatatcgct gtcaatttat tcgtagcttt aggtatcacg
     2101 ctttataaaa gtcctgttgt tttcatgttt ttcttattga ctgttcctct cgccgcattc
     2161 gttactctct ttttccgtaa aaggatcaga cagtccaact cagacttcag gcaagaaatg
     2221 gaagagactt ctgcaaaagt gatcgaagtg atcgaattag caccgattgc tcgagctcat
     2281 gggttagaaa aacacgaaac aactcgctta aataagcatt ttactcaaat ctatgaaaaa
     2341 ggattccact tagatatcct gcaatctgtt ttcggatcag tcagctgggc tagttttcag
     2401 atatttcagc tgatttgttt gattttcact gggatattag ctttacaagg gcgcatccaa
     2461 cccggcgatg tcgtgatgta tcagacgtat ttcacgacga tcgtcaattc cgtttctgga
     2521 atgatcacgc tgattcctac gatttcaaaa ggcatggaat ctatcacttc tattggcgaa
     2581 ctgttgattg ctggcgatgt agaaacttat cacggtaaga aaaaaatcca acatttggat
     2641 ggaaattttc gttttgagga tgtttctttt ctttatccgg actcactcaa accgattatc
     2701 gatcattttt ctctagaggt gaaagctgga gaaacaattg cttttgttgg gccatctggc
     2761 tctggaaaat ctacactcct taacttattg attggattta tccagccgac ttcaggaaaa
     2821 atttttctag atgatcagcc tttggatgaa ctggatatgc gaagtgtgcg aaattattta
     2881 gccgttgttc ctcaaaccac tttgttgttc tccgcttcga tcaaagagaa catcacttat
     2941 ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca tcgaagctgc gcaactttct
     3001 tcactgattg accagcttcc tgatggtctg gataccttag taggcgaaca tggcaacaag
     3061 ctgtctggtg gccaaaagca atgtatctcc attgcacgag cgttgatacg tcagccaaaa
     3121 atcattttgc tagatgaagc aacctcagct ttagataacc agtcagagaa aaaaatccag
     3181 caagccctga attacctgac agaaacaccc actactttta ttgtggctca tcggctttca
     3241 acgatcaaag aggcagataa aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt
     3301 acttacgaaa aactgttagc tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa
     3361 aaaataggag ctatgacaag acttttgtcg tagctcctat ttctgaataa acggtgttct
     3421 cctgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc tgttttttga
     3481 aattgttcct tgtaactcag agttaaacgg taggctcacg acctcttggt aaatggtgtt
     3541 caagagacag cctatcggca ataagaagca attttgtcat cgctcaaggc cgaacgtctc
     3601 tttcacaccc tctgttgttt cataaaatag ttttattcgc tgtaaaggtc gcgaactggt
     3661 gtcatgatga tacggttcaa gtcattgatg attgtactga agccttgctc tttttgcatc
     3721 aagtcattga ttacttcttc tgattggatt ttcacagcca tttcttgtgc tttcattgca
     3781 tcttcgtcgc tgaactcttg tccactcatt tgtttttctt gtaattcgat ttgcatcgct
     3841 tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt gtactttctc gtacgcttct
     3901 ttcaacgctt tgaattctgg taattcacgg atctcacgtt cgatttgatt cgcgctatcg
     3961 tagatgtttg acatgttatg ccctcttttc ttttagtagt tactctctat tgtaacatgg
     4021 ggttattcgt tttcaaaggg gctactctcc ttgatatttc cgccagaggt ctttagccgc
     4081 ttcaccaatt ccctgaacgc cttctttcac catatcgccg aagtttttca gcccttcttt
     4141 tgcttgttca ccatattgat tcaagttatc ttgccaattt ccagactctc cattagaatt
     4201 tcctgtgttt ccaacttgat ctgcagctac cacttgtcct cctgtggcat aggcatctgc
     4261 tacactaaat cctgtttttg aagtatacgg aaggatgcct tctgccgctg atttgaagac
     4321 tttcccgaca acatttccac ttgtcccttc caaatagtgc agcttgctgg attcttcgta
     4381 acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc
     4441 ttttgtggca tcgaaattag tttctgtggt tcctgttttc cctgctatcg tataacctgc
     4501 aggattcgca gcagccgctg ttccatttga aaatgtacca agaagcatgc tggtcatttc
     4561 atcggctgtt tcctttgata tgacctgttt tggttttttg tttgtgttat cgacgatcac
     4621 cgctccagtc gaatcaacga ttttcgtgat aagatgcggc gtatacatct gaccatcatt
     4681 ggcaaatgca ctgtaagcac ccgccatgat caatggagat actccttttt ccagtccacc
     4741 aagggctaag ccataatact tatcagattt tgctaaaggc aggccgaatt cttctgcttt
     4801 atcatatcct ttttgtaaac cgatctcatg caaaagccat acagcaggca aattcaaact
     4861 ttgtgcaaca gcttgataca tcggtacttc tccttggtat gtcccgtcaa aattatgcgc
     4921 atcgtaataa ctttgcggtt cgtctttcaa gatactggaa ggcttgtatc cagcttccag
     4981 tgctggtgta tatacgctta atggtttgat cgttgatcct ggtgaacgac gcatctgtgt
     5041 agcaaagctg aatcctctaa agacatgatc tcctcttcgt cctactagtg cttgtacacc
     5101 acctgtattc ggatctaaag ctactgaagc agattggacc atggcaccat ctgaagcatt
     5161 tgctgggaat aatgcactgt tttgataagt agcttctaat tgcttttgat aatcctgatc
     5221 taaagctgta tagattttat agcctttatt catgacatct tcttcatcaa gaccatactc
     5281 gttaactgct tcatcgatca ctgcatcaaa ataataagga taacgataac cagaatctga
     5341 attatcgtat gtgtcattaa gatacgcgct gatatctgtc tggctttctg cttgttcttg
     5401 ttcttttgtg atttttccgt tttcttccat tacacttagt actgtgtttt tccgatcatt
     5461 cgcatttttt gggtgatcga tcggattata aataccagga cctttcagca ttcctgccaa
     5521 agtagcagcc tcagacagcg taacattcgt tgcatctact ccaaaatact ttctggaagc
     5581 gtcttggaca ccccataccc cgttgccaaa ataggcattg ttaagataca tggtcaagat
     5641 ttcttctttg ctgtacttct tttcgatctc gattgccaaa aataattcct tcgcttttcg
     5701 atcaaacgtt tggtctaatg tcagatacgc atttttggca agctgctgag tgatcgtact
     5761 tcctccgccc cctgatgttc cgccagacaa gaccattcgg actgctgcac gggcaatccc
     5821 tttgatatca aacccgtgat gctcgtaaaa attcctatct tctgtcgaga ttactgcatc
     5881 ttggatatag ggagaaatcg cgtttaattc tacatatgtt cctttttggg cgtacagtgt
     5941 gccagcttct tcgtttttct tatcataaat gactgtcgat tggcttaatc ctgattttaa
     6001 cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag atacttgtca ctaagacaca
     6061 gatcaaacca atcagcaaaa tcaatttgtt cacatgatat tttttccaga tgcgctttcg
     6121 ccatcgatgg aattgtccga gatatggtct gatccaacgc caaaaactga tcaaagcagc
     6181 ttttatcttt tctaaaatgc tttgaaaatc catttgcttc ttctcctgtc tatttgattc
     6241 aatcgtctta ttttaccata gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat
     6301 atgttttttt acgttagtgc aagtagagta acataagaat gaacagaaaa atgtaagttt
     6361 tttcttaaat atgttttaaa aatggtacaa ttagtctgca attgcactga aaggagtaca
     6421 cttgtgaaaa tcacgatcac tttacctatg aaccatcctg aaacaacaat tcgcgacttg
     6481 cttgaaaagg aatggttagt tcctagaaag gttcgacact ttttgcgtat acgtaaaaac
     6541 gtttggatca atcaacaacc tgctttgttc catcatgaag taaagggcaa tgaccagatc
     6601 acattgttat tcgaagaaac agattataat tatcaagaag ttcaattagg agatgcgaag
     6661 cacatccgtg ttttatatga agatgagcat ttgattatag tggataagcc tgcaggaatg
     6721 aagactcatc caaatgaacc gacagaaaac agtaccttac tcaatcattt agctgcttat
     6781 ctcgacacta aacagcaacg accttacgtt gtccaccgct tggacaaaga aacaagcggt
     6841 gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac ttggaagaat gttggaacaa
     6901 aaaatgatct atcggagata tcaagccatc gtatggggag aaattgccaa agatatgacc
     6961 atcaccaata aaattggacg agatcgccat gatcggagaa aaagggtcgt agatgataaa
     7021 aatggtcaga gcgccttgac acatgtcact gtcaacaaag tactttctga acaaacacaa
     7081 gtctattgtg ttttggaaac cggacgtaca caccagatca gagttcattt agctcataag
     7141 ggacatccga tcgttggaga ccctctctac caaaatagaa cagctgaacg actgatgctc
     7201 catgcattag aactgcacat gatccatcca tttactcaag aaaaaatagt cggtaaagct
     7261 ttacctggat tatgggaata gaaataaaaa agaacagagt cttatcagca gataatgata
     7321 agactctgtt ctttttgtgt aatttgtctg cttacttttg atgttcgtgt tctgctaaca
     7381 gcaaactacc gataatccca ctttcattcg gcagtcccca acgaacgata tattcactta
     7441 cttgaggtgt ttccatataa ccacccatca attcaacaaa ttgctgacgg attttttgaa
     7501 ggagatgatc ctgattcatt accccaccgc ctaaaataat cttttcaggt gctaacgtca
     7561 acgtatagtt gatcagtgct tgagcaatat aataagcctg aatagcccaa acaggatgat
     7621 cttgcggcag atcttgccct ttgatccctg tgcgtgcctc aatagacggt cctgctgcta
     7681 atccttctaa acaatcttta tgataaggac aagtaccttc ataatggtca tctggatggc
     7741 gtttcaccca aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt tcttcattga
     7801 tcacgacacc tgcaccgatt cctgttccca cggtaaggta aatacaacta ttttttcctt
     7861 gtgctgctcc ttgcttcaat tccccatatg cagcagcatt gacatccgtt gtccaagcag
     7921 caggaatatt gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc cacccttttt
     7981 tgggagttgc caaaacgtat ccgtaattat cttttgtttt gtcgatacca atcgggccga
     8041 atgagccaat cccgattccc ttcaattcat aacgatcaaa aaattcaaat acctgatcca
     8101 atgtttcttc tggtgttgtg gtagggatac ttaatttttc taaaatttct ccaccatctg
     8161 aaaccgcaca cacaaatttt gttccgcctg cttctattcc gccatacatt cgttttcctc
     8221 ctcgaatccg attgtccgaa tctcataagg ttggattatt gggacaaccg cttgcttcgt
     8281 tggttcttca agcagattca atatgacagt ttttccattg tctttagtta tttccaattt
     8341 ttctaaatga ctagacatat tgaaacctct tactactact ttatcactaa acttgctgcg
     8401 tttcaaagct gtaattgcaa acgtttcact ttttatcgtt aaatattgtt gttttgaaat
     8461 caatgttcct tcatgatgtt tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc
     8521 atgcaaataa gtagagaatt tttcttctgg tccatgtagt tcaatgctgt aattgaaccg
     8581 atgctcacct agacactgtg cttctggtgt tggaaaatat ccccaatcgc ctagttctcc
     8641 cacactgcga agtagcgtca ctgcgatttg tccgtcttct gttacttcat attcattcaa
     8701 accaaagttt ccgactgtta caccgtactc ttcatcatga atattgacaa atgcttgctg
     8761 atgttgcgga ttcgtggggt tttcccaact tttagacaca tggtttgggc gcttcaccac
     8821 ttcaaaaata ctatctgctt cgtgatgttc cacatgtaac tttgtaggaa acaagacacg
     8881 taagcgatga tctttcatct gattgtcgag agtcgtttca aagtcgattt ttttactatc
     8941 ttttctgatt gtcatgattg tttttatttg aaggattcgt ttttctttgg atcgttctgc
     9001 ttttcgataa cgaaattcca caaccatctg ctgttctttt tctaaacgct cgtctgcaga
     9061 aacagggatt tccatttcct gtagaatact tatttttgct atttccggtg tatctactaa
     9121 tacttttact tcagagtttt ctttgttact ggataggatt ggctgatcac agaaaggttg
     9181 tttaaagata tactcatttc ctatatcgcc gatatcttca aaaatatgca gattattcaa
     9241 ctttcgtttg tttttgcgat cttccattgt aatggtacca tttttttcaa tcgtcaaatg
     9301 tagagggccg ttttcgatga ttcgtccact ctgatgaatc attgtttcct tttcttctgc
     9361 tttcgcttcc ccttctacaa gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa
     9421 tggtaaagtc actttgatca ttcttgccat atatggcaca cggaaacggt ctttcggcaa
     9481 atcataacca aattggacag tttcttcaga taaaatagct ggtatagtgg ccccactttt
     9541 tgtttctaca tgatacttac gtttaggcaa attttctaac tcttgatata gttgttcagg
     9601 tattccttca gcgaatcttt ttctttctaa ttcgatggtt atttctgctt caccagtttt
     9661 atccattcct gcagtattta caatgacaaa tggaaagctg tcttttggga atccggtcgt
     9721 atcgattgct gcggtcagct gttctaagga atcttgtgca agatactttc caacttcatc
     9781 tgctttttca aaacgaggta tcatttcccg atggacagaa tcgactgaac aaccgcaaat
     9841 actgtcgtga ggatgatttt gcattaatgt cttccaagca taatctaatt gatcatgggg
     9901 atagtttcca ctaacttcat aagccatcgt cgcaagtggt tctgtaatat tttctagctg
     9961 tcgctggacc ttggtattcc attgttttaa gtaaacccga gcagatgctg tattggctaa
    10021 agtataccag ccatccgttt cttgacttgt caattctcct tctactgagc ctaagtcttc
    10081 tggcacatct ttttgaactg cttccagata gtcagtaaaa ttagaatgaa taaactcgta
    10141 atctggaaac agttcatttg cgagatggat tgcttttgat atatcttttt gtaccggttg
    10201 atgatcaaca ccattcatca tcaataagtg gttcgtagag gcatattgtt ctgcatccgc
    10261 taatttttgc ttccaaaagg caagagctgc ttctttttct actggaatct cattcccgtt
    10321 gctataccaa ttggcaaata gcagaccaaa gattgctgtt tgatcagggc ctttccacca
    10381 catctctgag tattgggaag aatagttttc tgcttccaaa acttgattat caaagccaat
    10441 tggttttaca ccacggccaa acgctgctgc tgaaatacca gcttgtttca ttaattgagg
    10501 cgtctgtccc atatttccaa atgtatcggg aaaatagcct aacatgacag gggttcccca
    10561 tttacggctt tcttccatgc caatcagcat atttctcacg ttcgattccg agctgataag
    10621 aaaatcatct tgcaagatat aaaatggtcc aattctcaat tttccttcat tgattgcttg
    10681 ttggattgcc tgtctttttt ctggccttac ttgaagatag tcatctaaaa taatcgtttg
    10741 cccatccaaa tgaaaactgt tgaaggacgg atcaacttca aatagctcta gtagatcgtc
    10801 tatcaattca atcagtcgca tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca
    10861 gtggctatga gaaatgatat atactttctt tttcatttac gatacctcct acttttctac
    10921 tctaatatca aagtaatcca tgactaattc gcaaaacatc atatttgccc aagaaaacca
    10981 ttctctcgta taattttcag gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc
    11041 tgcgtcagtt gctacaagta aattcaatat ccgctctttt tcagatttgt ctgacgttgt
    11101 cattccttcc atagctaatg caatcggcca aacgtatttt tctggcgtat gggaggatcc
    11161 aattcctttt gcgtactttc cttcatagaa atatggattt tctttactca acaatgttct
    11221 tcttgtttgc agataatctt cctcttgttc agaacaatat cccaagtaag gtgcggcgat
    11281 cagattgggc acattactgt catccatgac tgtggcgttt cccattccat ctacttcata
    11341 tgcataaatc gtttgccctt tttgattaac cgttcgtccg tgattctgaa tcccctcttg
    11401 gatctctttc ttcaatttct gtgtttgctg tacaatttct ttatcatcga gtatcgtttg
    11461 aaaaatttct tccacataac ctaacacgac caccgcaaac atattggatg gtaccagata
    11521 tccataacgg caagcatcat cgctaggacg gaaaccagac catgtcattc ctgtaaaagc
    11581 tgtttttgca cctcgcccgt cattgatcag cgtatcttct tttcttgtcg tgtctcgtac
    11641 aaatgtataa ggagagtgtt catgatcttg ctctgttttg aacacgtcca agattttttt
    11701 gataccttca ataaaagatt gattgaattg atcagttttt cctgtatttt tgtaaagaag
    11761 ataggccaac tgtattgggt agcataaaga atcaatttca tattttcttt cccagatcca
    11821 atcgttcatt tttgtatgat ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc
    11881 attcgcatat ggatcgatat tgatataaaa aaattgcttt ttgactaacc cgctgatcat
    11941 gtcagctaat tcctcatctt cttttgcaat cactaaataa ggacgaactt gtgcagtaga
    12001 gtctcttaac cacatcgctg gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg
    12061 ctttttgact gttgtcaaca gagtattggc aaatgctgct gtaaaattct ttgcccagtc
    12121 cgcatggata tccccacatt tttgtgtgat atcttccata aatttctgca ccgattttgg
    12181 tatatcttta taaaccacta ataaaacctt ctttctattt gatatatctt taaagattat
    12241 acttcataca gccaattatg tctacgcttt caaaaaatat ctttttattt actttttcat
    12301 ccattatggt attatttgaa tatgatatat cattaagagg tgtttatatg actcaaccat
    12361 tgtaccagac aatatttaat gatttgcaaa cggctatttt aactggtgaa ttacctgtag
    12421 acagccaact accaactgaa aaagaacttt cacgtaccta taaagtaagc agaatcactt
    12481 ccaaacgtgc attgactgaa ttagagcaat taggacttat ttatcgcgtt cgcgggaaag
    12541 gcagtttcgt taaatccgct tctccggaac agattcttgc ttctcctaca aaaacacgac
    12601 ggattctgtt tcttcttcca tatgtcgctg atttgtctgt tggtgatttt agcaagggat
    12661 tgaaccctgt gatgcaagat aaacagattg atgtcatgat gacgacgctt gactttctac
    12721 aaaataaaac agcaaaagag ctaatcaatg aatttgacgg attgatttat tatgctcttg
    12781 atacagaaca acatcttgac ttactttttg agctttcatt gaaagaattc cctgtcgtca
    12841 tattagataa aaaaatctat gagttacctt ttcctaccgt tctttccgat aatttccaag
    12901 gcggtgcgct tgctgcctct tatttgatcg aacagggaca ttcaaaaatc gcttatttgt
    12961 ttggtacgca gactcatcca caatccgttc gacagcgtta ccttggttac cttgaagcat
    13021 tggacaaagc tggtttgact ttccacactt cactggatga caaaagcgcg acattagatg
    13081 aattgctttc ttatatcact aagtatgaag taactgcttt tgtttgtgaa aacgatgttg
    13141 tcgctatcca gaccatgaat ctcttgagac agcatggtta tcatatccct gaagattttt
    13201 caattatcgg atttgatgat atccaagcag cagctcttgt tgacccgcca ctgacaacca
    13261 tcgcacaaga ttttgaacag ttaggtaagc ttgcgggaga tgctttggtc acttggttag
    13321 agacaaaaga tcgaccatcc gatagtaaat ttccagtttc attcatcaaa agacaatcaa
    13381 caaaggagaa ataaaatgaa tatcggtcaa atagatacta gacatgcaac agcgaatcag
    13441 ccaaatttct ctaatgggaa ctgccagcct tatactggcg ttccttttgg gatgaattat
    13501 tttgcaccgc aaactacgga tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt
    13561 tttcaaggct accgtcttac ccatcaacca agtccttgga tgggtgattt cagtcatatg
    13621 ctgtttacgc cgatcaatgg aaaactacag gagaacaccc ttttccatgc tcaaagttct
    13681 taccgcccag aagaaagtat attttgcccg actcatctgt cgatctgtca attacgttat
    13741 ggcatctgtt ctacattgat acctagcatg tatggcggaa tcttatctat tgattacagc
    13801 aaaaatgaca gcggtttact tttatccttt ccgggacgcc atcagctctt tgttatagat
    13861 ccttacacag tggaaggaaa aatcacacat tttgctggtt cagaagatcc tgatttttct
    13921 ttttatttta tattgagatt tgaatatcct ttaacaattg atgagttagc tgtttccaac
    13981 aaaaatagcg actcgatccc gttgtatttt gaacagacaa aaaaacaaac tatccgattt
    14041 gggacatctt ttatcagctc ggaacaagca gcttataacc tggaactaga acaaaaccaa
    14101 agcaaagaga cttaccttgc tcacagtcaa gaacaatggc agtcctattt tgatcgtata
    14161 cagctcacac accatgatca acaacaattg cggacgtttt atcataattt atatcgggtg
    14221 tttttatttc cacaaacatt ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat
    14281 acgactagtc gatctataaa acctggtgtt ctctatacaa ataatgggtt ttgggatacg
    14341 tataaaacgg tttatcctct ttattctttg attgctgtag aaaaatacga agaaatgctg
    14401 gaaggttttt tgaacagcta tagagaaagt ggctatttgc caaaatggct gtctcctgat
    14461 gaacgtggac ttatgcctgg gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa
    14521 gatatccgac gagacttgat gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt
    14581 caaagcaaac atccaaatta tggacgccaa ggaacagaag attacttgac atatggctat
    14641 gttcctaatc actaccacga atcagtcaat cacacattag actatgctta tagcgatttt
    14701 tgtattagcc aagttgccaa ggtactagaa gaagaggaaa tgacgaagaa atatcagcag
    14761 caatcattga actatcaaaa tatctttgac tatgagtcag gatttatgcg ttcaaaagat
    14821 aaagatggca agttcagaga accattttca gctaatcgtt ggggacaaga ttatgcagaa
    14881 ggaagtgctt ggcaaagcag ctttgctgtt tatcacgatt tttcaggact gatcggaaaa
    14941 tatggtggca gtgagagata taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg
    15001 tttgacccag gaggatatgg ttttgagatc cacgaaatga gtgagatggc cgcgatagaa
    15061 tttggacaat tagctatttc aaaccagcca agttttcact atccttactt gttcagttat
    15121 ataggacaac ctgaaatggc acagccactg cttaaacagt tgatgactca gaccttcgac
    15181 gattctccaa tgggttaccc tggagatgag gacaatggaa gtatggccgc ttggtatatc
    15241 ttcaatagtc tgggatttta tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg
    15301 ccattaattg acaaggctgt tttgcttttg tcaaatggaa aggaattgac gattgaagca
    15361 acgcctaatc ggccgcagca acaatttatc cataaaatca aacggatgga cagagaatat
    15421 acgaaattat attttaccca tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta
    15481 gggatcatcc ctcaccctaa gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac
    15541 tataattaac aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaagc
    15601 aaaaatagat cctcatattt ttcgtttggt tggtcttatt accgcagatg caagcttttg
    15661 aacaccgttt attgagaggt tgtgaaatca gcgttctgac ctgagtatta gagagacaaa
    15721 tggcaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt
    15781 gaacaccatt tagtggagag gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat
    15841 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga gaaa
//
LOCUS       DAGCXG010000054        15863 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGCXG010000054 DAGCXG010000000
VERSION     DAGCXG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.057"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(89..988)
                     /locus_tag="MB022_001731"
     CDS             complement(89..988)
                     /locus_tag="MB022_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBT4466729.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(998..1354)
                     /locus_tag="MB022_001732"
     CDS             complement(998..1354)
                     /locus_tag="MB022_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4466730.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1709..2005
                     /locus_tag="MB022_001733"
     CDS             1709..2005
                     /locus_tag="MB022_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBT4466731.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2649..3602)
                     /locus_tag="MB022_001734"
     CDS             complement(2649..3602)
                     /locus_tag="MB022_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4466732.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3625..4380)
                     /locus_tag="MB022_001735"
     CDS             complement(3625..4380)
                     /locus_tag="MB022_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4466733.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4377..5336)
                     /locus_tag="MB022_001736"
     CDS             complement(4377..5336)
                     /locus_tag="MB022_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4466734.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5329..6279)
                     /locus_tag="MB022_001737"
     CDS             complement(5329..6279)
                     /locus_tag="MB022_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4466735.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6429..6881
                     /locus_tag="MB022_001738"
     CDS             6429..6881
                     /locus_tag="MB022_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBT4466736.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6970..9237)
                     /gene="gshAB"
                     /locus_tag="MB022_001739"
     CDS             complement(6970..9237)
                     /gene="gshAB"
                     /locus_tag="MB022_001739"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBT4466737.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9424..10422)
                     /gene="ftsY"
                     /locus_tag="MB022_001740"
     CDS             complement(9424..10422)
                     /gene="ftsY"
                     /locus_tag="MB022_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBT4466738.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10433..11242)
                     /locus_tag="MB022_001741"
     CDS             complement(10433..11242)
                     /locus_tag="MB022_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4466739.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11251..14832)
                     /gene="smc"
                     /locus_tag="MB022_001742"
     CDS             complement(11251..14832)
                     /gene="smc"
                     /locus_tag="MB022_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBT4466740.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14852..15538)
                     /locus_tag="MB022_001743"
     CDS             complement(14852..15538)
                     /locus_tag="MB022_001743"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBT4466741.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
BASE COUNT         4467 a         3360 c         2762 g         5274 t
ORIGIN      
        1 gcagcatatc cacgataaat ggatctactg ctcttactta tccttatgtt ttacaaggta
       61 ttttctttta cacattacta atgctgtctc aatacggaac gattgtcatc gcttgttcaa
      121 attcctcttg caaatgttgt gtttgatagg cctgaaaagc tgggtgatgg gcacgcaaga
      181 ggacacgtaa tttcatagga tcatagctgt ctagcaactg ttctttcaag acaaaatctc
      241 gggtcagctg ccattcataa taataaccaa aaggtctgca gctgtaaatc gtgtgaccga
      301 tggatttatc ttcaaagcga cgatgcgtat ggccaaaaac tacttgtttg atatttgaat
      361 aacggtcaag taattctccg aatgattctg atcctaaaaa tgcatttaat ttgttccaac
      421 gctcataagg agcagattga tacatgataa atgcccgttt aggaacgaaa tgagtggata
      481 aaatcacctc aagttttttt gcttccagct ggtctagcaa attttttgca gctgcaagca
      541 tttctgcatt gacctcgaga tctgttttcc cacgctggat gaaccgatca taccaataca
      601 aacgcttcat tcgtacgatt gcctcgtaat ctttcaattc ggaaaactga tagtcgtacc
      661 agccattcat ccctaaaaga acggtctgct catttaaagc aatataccgt tcatttaaaa
      721 aatgtgggtc tggaaaatgg tccatcatct gttcattggg cacatctgcc aactcgtgat
      781 tgccgaaatg aaatgtcgta gataatcctt tattgacgaa aaattcgaca acagctaggc
      841 aacggtctac tttattagcc gtatctccgg ctagatgaag ccgagtgatc ttgtgctttt
      901 gcaaaacttg ccaaagctgc atcagctctg attcgccgaa tttattgatg tccacatgta
      961 ggtcgctgat gatcgccaat tttcccattt tatccctcta gtcttttcga acaacttgca
     1021 agttgccttt tctcaagccg cttggtcctt ttaattgtaa actattataa gttggttcta
     1081 aagcaagtgt ggatggatca tccatgtaaa gtattgcact gtctttgatt ttgattggtc
     1141 caactggtgt atcagccact acagaataaa gacttagatc tgtttgtgta tcttttttga
     1201 caatgatcaa tcgaaatgcc gtatccaatt ggcaactgac ttggctttct gcaaatggac
     1261 cgtcaccgtt ttcgatatct aataaaagtt gatcgtctgc ttctaattta ttcttcaata
     1321 cttcttgtgc ttcttttgtc acttctaatt tcatctgtat cacctcatgt catagaataa
     1381 tcactttatt catcagatgc aactaatttg tctggttctg actgttctca atttattgga
     1441 aagtaagtca ttcgtttatt atgatttctc ttatcacaaa agagacaact ttcccttttc
     1501 ttcgttcagt ttcttgaacg gaaaaagacc caaaaagcaa cctgttattt gtttaacctt
     1561 attcctttct taaataacaa aaaatactat aaaaaataaa aaacctttaa caactattgg
     1621 aaaaagaatt actttttgtt cacggattat taacaactct tcggtagagt aaacttatca
     1681 aataaaagac ttctttggag gaatcactat gaaggaatat ataaaaaaca tctattttat
     1741 cgaagaaaca caaaatatcg agggttctta tattgaagta aaaacactct ttgtcaatga
     1801 agataaaacc aaagcattgg atatttataa aaaattagct tcaaaaaaaa cgaatagttt
     1861 tggattgata ttaagcgagt ataaaatcaa agcagaagag agttatttct atcaattact
     1921 aaaacggtgg agtaagcttc ccgccgattt ttatcgaaaa atgcagatca tcaactatca
     1981 accgcttgct gagacacatg cctaaataga ggttgtgaaa tcagtgcttt gagctaatac
     2041 cgaaggtcag ctgattgaac attgtttaga agagattgtg acaactgcga tttgctttga
     2101 gtaataagaa cgctggacag aaaatagctt ctcatatttt ctagtctagt gggcttattg
     2161 ccgagaagca gcagatggaa catcgtttag aagagattgt gacaactgcg atttgttttg
     2221 agtaataaga acgctggaca gaaaatagct tctcatattt tctagtctag tggacttatt
     2281 gccgagaagc agcagatgga acatcgttta aagaagagat cggtacaaaa taaaaatatc
     2341 taaatttaag ttttatctaa gtttcgcttt gtacctttga ttcaaatcaa aggaggcgat
     2401 cttgatgctg aaaaaatgga tcacactatt ttttgccact ccccattcta gaatcaaccc
     2461 gtatgatgta gataatgaat tcacgttgaa aaaagatttc tcaaataaat aaaaaccatc
     2521 cgtaaaatca tcaatacgat tttacggatg gtttttgata acttctttga ggataggctc
     2581 ttgctattta agaatgtaag aaacattatt gttacatagt atgagagttt aatcctctgt
     2641 tatttatgct atttaaatgc ttgattcaca tcttcgatca tcagtttcat tgattctaaa
     2701 ccgcccccgc ttaaatacca gacatctggt tcaagggaga tgatctgctg atttttacca
     2761 gcattcgttt gtgctaccag ttcattggca gaaatatcat ctttgctgtc gtctccacca
     2821 atagcttttg ttctgtctac tacaaataag atatccggat ttttttccag cacatattca
     2881 taagaaacac tttgaccgtg agtggatgct tcgatttgat catctgcttg ttcaaagcca
     2941 aataaatcat ggatgaaacc gaatctagaa ccagatccgt aggctgataa ttgcccttca
     3001 ttcacaagtg tgactagtgc ttttttgtca gtagcttctg ctttcttttt cgtctgatcg
     3061 attgcactag ataattcctc taattgagtt ttagcttctt cttgcttacc aaagatctcc
     3121 cctaaagcag tgacattttg ctggaaagat tcccatggat ttttcgcatc taaagaaagg
     3181 aaaatggtcg gagcaatttg acttagatct ttttgaaaat ctctttgtcg gccagaaatg
     3241 atgattagat ctggctgaag ctgattaatt ttttctaagt ctggttcttt tattccacct
     3301 gctgattcta cttttttata ggatgaaaga tactcaggaa ggctgcttgt agctgctcca
     3361 actaccttat ctccaacacc aagagcatcc atcgtatcta aagaaccatt gtcgaatacg
     3421 acgacttttt ctggattatt tggcacctcg atttggtctc cattactatc tgttatcgtc
     3481 aatgtagttt cagtttgtgt gctgcttttt tttgtgtctg cctgactggt gttgttgcta
     3541 tttccacatg ctgctaaacc aaatgatgct agaatcaaga tacctactac taattttttc
     3601 atctcttctc tcctaatgtt agttttagaa ataaagacag aatctgcggc cttcaatctc
     3661 acaaatccgt atgtccattt cgtataattc atccaataca tcttttttta tcatttcttg
     3721 ggtcgttcct tgcgtaaata actgtccatc tttcattgca acaatttcgt ctgcataact
     3781 tgcagcaaag ttgatatcat gaagaacaat aacaattgtt ttcccgattt cttgtactaa
     3841 attttttaaa atctgcatga tttgaaccgc aaaattcata tctagattgt tcaaaggttc
     3901 atccagtaaa atataatccg tgtcttgcgc tagcaccatt gcaatatacg cacgttggag
     3961 ttgaccgcct gaaagagtgt caatcgattg atccgctatt tcttctaacc caatgtgttc
     4021 cagtgattcc tgaacgattt cctcgtcttt tgtcgtcaat tttcctttcg tataaggaaa
     4081 tcgtccaaat tctactaatt ctttcacggt gatttggaca tttatttcat tcgattgtcg
     4141 taggatcgat aattttttgg ctaattcatt ggatttccaa gcctttactt cgttatggtc
     4201 taaatagatt tcacctgtat cttttggtat caaacgactg atcatcgaca acaccgttga
     4261 ctttccagca ccatttggac cgataaaagc agtcaacttc ttttcaggaa tcgttaactg
     4321 tacatcttta accactgatt ttccatggta cagtttattg accttttgta aatcaatcat
     4381 aggatactct tcctttcgcg aatcaatttt gtgacaaaat aaattcctcc gacaaattca
     4441 acgacgacac tcactgttgt attccagccg aaaatctgct ctactgcgag ctgtcctcct
     4501 gtcagaaaca gcatagctac cagaaagctt ccgataaata gctgaccatg acgataagta
     4561 gaaaagacac gataactgat atttgcgaca ataaacccta aaaaagtaac tggacccaca
     4621 agagccgttg atgtccctgt caaaagactg acacaacata aaatcaaaaa ttcaaaacga
     4681 tctgtgtcta tcccgaggtt tactgcttgt tcatttccga gatggaaaac gtctagcaca
     4741 tgtcgatatc gaaataacag gtaccagcag ccgataatga tcaaagaagc aggcaaaaga
     4801 tactgactct ggatattgcc aaagcttgca aataatctgc cttgaagcaa atcatattca
     4861 ttcggatcca tgatgacttg taaaaaggta ctgacactac caaaaagcgt acctaaaatc
     4921 atccctgaca tcagtaataa aaaaagattc gctctcattt ttttcaaaag aaagaaaaat
     4981 aacaatagac tcagcccgac catcagccca acgttcaata agaaaaaaac tgtacctgtc
     5041 tgctgaagca tcgtcactcc tccgataaag aagaaaagga ccgtttggac aaggacatat
     5101 aacgactcaa gtcccaatac tgaaggagtc aaaaactggt tatgcgtaat tgtctgaaag
     5161 ctgattgtgg cactgctgac agctagcgag acaaggataa atgcaaaaat tttcttactc
     5221 ctcaatgcac atgcaaaatg ccagtttcca tatgtatgga tcgtcaagta cattcctgta
     5281 acaaataaaa caagaacaag cataccaata aaaatagatg gctttttttt aagcatatcg
     5341 tcgtcccctt atcagaagat aaaggaagat agccccaccg atgattccaa cgatcacact
     5401 gactggtatt tcataaggaa aaatcaacac gcgagcgaca agatcgcaaa atactaagaa
     5461 caaactacct gctaaagcag ttacgccaat tgttttgtgc acttgatctc catatattct
     5521 agagacaaga ttcggaatga tcactccgat aaaaggaatg cttcctacca tgatcaacat
     5581 gactgcacta gccaatgata ctaagccaat gcctaaaaac tgcatcttct ggtaatctat
     5641 tcccagattt actgcaaacg attcgcccag accaatgatc gtaaatcggt aagcaaatag
     5701 ccaaaccaag agcaacaatg gaacaacagc atacaataac tcataatttc ctcgtatcac
     5761 agtcgcaaaa ttcccttgaa gccatgaagt catattttgt accaactgga attgataagc
     5821 aaaaaaagta gcaactgaac caatgatatt ccctaacatg actccaatca acggaagcat
     5881 caattgattt ttctggagga acaatcgcgt aagggataaa aaaagcaatg ttcctgcaaa
     5941 agcaaagcaa aatgctgtca aggatcggac aatcaaggat gcaccaggta ggaaaatcat
     6001 agccactaaa atacctaaac gcgcactatc catcgtgccc gcagtcgttg gtgaaacaaa
     6061 tttattttgg gtcaaatgtt gcatgatcaa cccacaaata ctactggtcg ctcccacaag
     6121 gatcaaacaa atcgttcgtg gcaaacgagt cactagcaag atttgtaagc tttgcccatc
     6181 taactggagc agttctgaca attgtacatt cgatactccg atgaacaatg aaatactact
     6241 taaaataact aataatgcta ctagtcgtcc gtagcgcatc caatccacct ttctaattga
     6301 gaatgatttt cattcttatt ttaagtgaat cgttgaacaa aagcaaccgc tttttcatat
     6361 gttttaggta ttattgggaa tatgctataa tcgtaagaaa gacaaagaga agaaggaggg
     6421 tattttttat ggcacaagat gatgtaaaaa gccatttgga taagggactt tatggtacac
     6481 ctttagtgaa ccctgaagaa cagcataaat atttagggac attcagggaa cgctgctatc
     6541 ttagtatgac tgttgctgaa atgatccaac caaaaaacaa agaaaacttt ttaaaagaac
     6601 tcaaaaaaca tccagatgct tccatactct tgaatggggc tctacccctt tctgtccaaa
     6661 accaatatat ccagctagca acgaaaaaca atagccgttt cactgtagtc aacgattacg
     6721 tcactgcaaa tcctgaggaa ttcggattgc tcttaacagc aaaagaagca gtaaatgaac
     6781 ctgtgattga tatcgaaaaa aaatacccag aagaaaactc ttcgccagct gaaaaagaaa
     6841 aaccaaaaaa gaaaaaaagc ttctgggatc gtttatttta aaacgaaaaa acacataaat
     6901 ttttacatgc aaaaaaagct tttgatcaga ttgacatcaa ataggacaat ctgtcaaaag
     6961 ctttttcttt taaaatactt caggatataa caatttcaat acattcatcg tcaatcttct
     7021 gccttttcct gcaaaaggat aaacatgcat atgcatggct ggattgaaat ttgcttcgat
     7081 gatcccatat gcttttttat cagttgtagg atcgatttct ttatctggta taatcaaatc
     7141 gataccacta atcttagctc ctagtgcttc taccgcagaa acggcgattt ttttgtaagc
     7201 ttcagaaaat tcatcagtca tatcgatcga atcaccacct gtactgatat ttgagttctc
     7261 ccttagataa acaatttgat tggcttgggg aactgattcg atcgtcaacc cttgttcttt
     7321 caacatcaat tgttctaatt cacctaattg gatcaattct aaaggtgcac gatgattcgt
     7381 tccacgcaaa gggtcactat ttttttcttc aacaagttct ttcactgttc ggatactgtc
     7441 accaataaca ttagcaggga ctcgcaacat gatggcttgg acttctccgt cgatcacaaa
     7501 gaaacgatat tccgttcccg gcataaattc ttctactaaa accgaactgt cttcacgaaa
     7561 agcaatggct aaacctgctt gataatcttc cagactagct ccttccttga aaatcgtgat
     7621 acctaacccg taattggttg atttaggttt gatcacaaat gcctgctcgg caaatctcgg
     7681 ataagctttt accgcttctt ccatggatga aaattctgcg cctcctggta cccggaaccc
     7741 agcttctttt aagacttttt tcgttactgt tttgttttcc atgataagtg ggacgatata
     7801 gctgtcctta cttgtcatgt ttgcattttt cacatattct acatggtctt ggaattgcag
     7861 acgcaaaaat tgatccgact catctaatac tttgacttgg acaccttttt ggatggcatc
     7921 gaacataaat atctgagtag aaagttccat ttcgcgaaaa ccggccaact gatagggacg
     7981 ttcatggctt tctttataat attgatgtcc caatgccaca gcaacttctt gctgactcga
     8041 tagctgtgct tcttgataga gtttcgctgc taacgtttgt tcaggatgat ccatttgagt
     8101 caatgcttcc tctactaaat aaagagaatc caaccaatcc aattcaacca gcatttgctt
     8161 cattccttca agaatttctc taccttcttg atgaaattct gtttgatctg atggttgttc
     8221 tagtgctact ttgttatttc gagtcgtacc ttctgctacc cattggtcac agtcttcttt
     8281 ttcttctgtc cataacatgt acatcaggaa taattgtaag aaccgaactt gctctggatt
     8341 gatacccaat cttgcataag gattcaaatc gatattccgc aattcaatat aacgaacacc
     8401 tgttgttgct aaatctgcga ctttctttcc gcctcggaaa cgaagcggtg tataaaattc
     8461 tttctcctcc gaaagtttcc cttcttcgat cattcgctcg atatctgcta aatactgttt
     8521 catagaagca taggaaactt tcacatttgg atgattcgta tagcccagcg cactgttgcg
     8581 aatactgcga acaggttctt gcggatgtga ttcatctaaa aagtagttct tctcactcat
     8641 tggtgaagca ccgaataaat aagtgatcat ccagcgatag cgcataaagt ttctcgctgt
     8701 tttcaaataa agttctgttt tgaattcgga aaaatcttga aattcttcct gatggctgaa
     8761 caatgttctt agcaactcat cgccaaattc aaaattgaaa tgaataccgc ttaccatctg
     8821 ttttcgcttc ccgtattctt ttgctaaata acgtcgatac aagacatctt cgaaattttt
     8881 taattttgca ataatgatct cttcgtcttt ttccggtaag gcaggtggca tacttaatgg
     8941 ccagatcatc tcttctttcg gtatagaacg agcagtcaca tcataaacag cagcgagata
     9001 ctgaaataat tcgggaatag aatcggtaac aggggtgatc atctctgttt gtgtttcgct
     9061 aaaatctgtt tggatatagg ggtgatagga tcgatcgcca aagattgctg gatgatcggt
     9121 ttttgctaga tttcctgcaa gatcaacccg ctgtccttcc cgttctatac caaatcgagc
     9181 ttggtcgata aaagggcgcg cattgacatg caataataat tgcttaaaat tcatcattaa
     9241 gtgacctcca attcatatga atctaattat aatcaaaaag tctaagaaaa agaacgaaat
     9301 tgtttgagaa ccgcttacaa ataagtataa aaatgaaggc atcaaaaaca cagctatgtt
     9361 aaaaacaatc attcaaacaa ggagctgcaa cttttgtcac agctcctgct ttttagacat
     9421 gggttattct tcttttagta atcctttgaa caatccaacg acaaaatcat ttggatcaaa
     9481 cggctccaag tcatcgattc cttcaccaag accgaccaat ttcactggta aatgcaattc
     9541 attacggata gcaagtacga taccgccctt tgctgtacca tctaattttg tcaaaacaag
     9601 accggtcaca tctgttgttt ctttgaattg ttttgcttga accatcgcgt tttgaccagt
     9661 ggttgcatct aatacaagaa gtgtttcttg gggtgcttct ggtaattccc gctggattac
     9721 tcgttttatt ttttctaatt cgttcatcaa gttcacttta ttttgcagtc ggcctgcagt
     9781 atcaactagt aaaacatctg cctgctctgt ctttgctctt tccaaagcat cgaatacgac
     9841 agctgctgga tcgcctcctg cattgccacg aacaacttca acaccagcgc gttcgcccca
     9901 gacaactaac tggtcgattg ctccagcgcg gaaggtatct gctgctgcca tcaatacttt
     9961 tttaccttcg tttttgaact gatgggcaag tttaccaata cttgttgttt tccctacacc
    10021 attaacaccg acaaacaaaa taactgttag tccgtcttct tggatattta gttcatttac
    10081 ttcattgatt ccttccgctt catagagatc cacaagtttc tcgatgatcg cattttggac
    10141 ttctgccgat tttttcgcat ttctgagttt cacttcttga cgtaatgctt cggtgattcg
    10201 tatcgctgtt tcaaaaccta catctgcacc gataagtgtt tcttctaatt cttcaaaaaa
    10261 gtcttcatcg actgttcgaa aattggcaaa caactcattc atccgttcac caaatgtttt
    10321 acgcgtcttt tctaaaccct tgtcgtactt ctcctgtact tcaacttctt ctgttttctc
    10381 ttcgccagta aaagcacgct tgattttatc aaaaaatccc atttgactcc ccctactcta
    10441 atacaaattt ttggatcact ttggctacgc cattttcttc attgctggct gtcacgaaat
    10501 ctgctgcttc tttgacgaaa ggcacagcat tctccatcgc tacgcctagt ccagcatatt
    10561 ggatcatcgg taaatcattt tcctcatcac cgatcgccat gatttcttct tgtttcaaac
    10621 caagatcttt tgccaataaa gaaataccat aagcttttgt aacacctttt ggcataaatt
    10681 ccaaaagcat acttcttgtt ttgatgatct catacatttc atggaattct gctggtattt
    10741 ctttgatttt aggatctaat tcttcttggt cataagcgat tactgcctta ttaagcacaa
    10801 attcatctgt aatatcgctt atatccgctg gttggaattg cagcagctta ttcaacacat
    10861 tgtacaagga ttgcttcctt ggggcactag gcagctgtaa aaccacattg tcagacaaaa
    10921 catctagtgg cagatccaat tgctgagcca gtttgtataa gcgttggata tctgatactt
    10981 ccatcacttt cttttccatg atttcaccag tatcattttt ttgtaccaaa ccgccgttga
    11041 aagtgatgct gtagtcacct tcatctacca gtcctaattc ttgcagataa tgcgccatcg
    11101 cagctaaggg gcgtcctgta catagaacga tcttgacccc ttgctgtttt gcttgtgcta
    11161 atgcttgttt attctcatct gaaatcagct tttgttcatt taataatgta ccgtccaaat
    11221 cgattgctac taatttgatc attttcgttc ctacactttc gtttcgttga ttttcgcctc
    11281 ttcattgata tcttccatcc gtacagaaac gatttttgat acaccggatt cctgcatcgt
    11341 cactccgtac agtacatctg ctgcgaccat cgttcctttg cggtgtgtga ctacgataaa
    11401 ttgtgtgtca tcttggaaat cgctcaagta acgaccaaaa cgtttcacgt tggcttcatc
    11461 taacgctgct tctacctcat ctaagataca aaacggaaca ggacagacac ggataatgga
    11521 aaagagcaac gcgatggctg taagtgctcg ttctcctcca gaaagcaaac ttagactttg
    11581 cagttttttg ccaggaggtt gtacttcaat ctcaatacct gttttcagca aatcagttgg
    11641 ttccgtcagt actaattcag ctttaccgcc gccaaacata tttggaaaga caattttgaa
    11701 ttcttgacgg atagcttcaa atacttcttt gaatcttgtc cgcacttcat catccatctc
    11761 atccatcgtt tcaaacaatt ggtttttcgc tgctaacaga tcatctcgct gtgttgctaa
    11821 gaatgtatgt ctttcactga cttgttcgta ttgttcgatt gaatttaagt ttactggtcc
    11881 cagtttttca atctgttctt ttaaggatga aacctttgtt ctactgtctt cgatatcggt
    11941 tgtttcttga tagtcttggg aagctttctc aaatgtcagc tgatattctg actgcagata
    12001 ggtcaaatga ttatctaaaa tcatttcagc tcggtctttt tggacttcta atttggattg
    12061 ttccgacagt cgtgcttttt gctcacgatt acgctctgcc aagactgctt caagcttatc
    12121 gatctccttt tgccttctat ctcgctgctc tttggctttt gccaactcgg cttttaatgt
    12181 ttctcgctga gcagataatt cgttaatttg tttttccaaa ctttcttctg tgacttcatg
    12241 atcagaaaag tctgctgtca atgtcgccaa ctgcttttct atcgcttctt gacgttcggt
    12301 tgcctctgct ttttggactc gggcaccgcg gagctggatc tgcagatgat taaactgctc
    12361 tttcaagaca gcaagatctg cttgttcttg tgctttttga ctttgtactt gtgctcttct
    12421 ggcttccatc tggtcgcttt cttgacttaa cgatttgatt tcttcgtcga ttttttgtcg
    12481 ttgcgattca agatctgttt gtttgtcttt caattcgttc gtttgctttt cgtaatcttc
    12541 gataaactgc tgcagttcac gtgtctcaaa agttgagatt tgcttttctt tttcaaaacg
    12601 ttcaagttca tttatgatgt tttgtagctg atttgtcgct tcttgttctt caaaacgtaa
    12661 ttgttcgcct cgtgtacgca aaacttcttg ttcttcggct aaacgagctg tttcttgttg
    12721 cagctcctga actttttttt cttgtgcttg gagttgttta tctgcttctt caaattcact
    12781 agtcaactgt ttcaattctt gattttgtac aaacaaactg ccttggtttc ctcgtttatt
    12841 cgctccacct gtcatggatc ctccggcatt catcacatca ccttctaagg aaaccactcg
    12901 atattgatag cggatcgttt gcgcgattgc attggcacta gttaagtctt tggctaaaag
    12961 gatcgttcct agcaggtttt gaaccacagt ttggatttga tcgggataac tgacttgttc
    13021 actggcaatc cctataaacc cttctacagc tgctgcttgt gtcaatatat gagccggtaa
    13081 ttgtcttggt ttgattgtcg tcagtggaag gaatgttgct cttcctcctc tttgctgttt
    13141 caaatacgtg atggcttgcc gagcatcttt ttcattttca acgatgacat gctgggctgc
    13201 cccacctaaa gctgcttcga tagctaacgt gaaatctgca ggaacatcga tcagttcagc
    13261 cactgctccg acaatacccg aaagctgctg tttatgctgc aagaccaatc gaaccccttg
    13321 gtaaaaacca aaatagtttt cttggatttc ttgaaggctt ttcttacgag cacgtagctg
    13381 ctggacttga ttcattaatt gatacatttt aggctgttct tttgctaatg cttcttggaa
    13441 ctttttcccg ttttgttgga tcatttctaa cttctctttt gtttcagtta aagatgattt
    13501 tagctgcgcc tgcttttgtg tcaattcttc tttttgtaag atcaatcggt ctactgatgc
    13561 ttctacttcc gattgcttgg ctaacgtctg cttgcttttt gctgtttctt gtatatattg
    13621 acgttctaga tacttcagtt cgtttcctac agctgcttgt tcttgcatca aatcaacata
    13681 ttgatctctt aattctgcca ataattcttt cgttgatttt tggtattttt ctaattcttg
    13741 ctgtgttttt atgagatttg cttcagcttt ttggatctct gtttcttttt ctgctgctgc
    13801 tttcatcaag ctttcttgaa gcttttcaaa atgtttaacc tttttttgtg cttctgctaa
    13861 tgaagtttga tattcttgcg agcttttttg tgtatgcttt gtccgctcct gcaaaacatc
    13921 tttttgtcct tctgtctgct tcagtttttc cgaaagatct aataatactt gctgattttt
    13981 ttcgatcaga cgatcggctt gcgcattttc tttacgctgc ttcgccaaaa tggattcttg
    14041 ttcttggatc gactcagata gtttgcctaa ttctagattg aatttcgcaa gctgtgcttg
    14101 tttgttgtcc caatcttttt ttgctgtctt gatttcagca accatcaaag agacatcggt
    14161 ttgagtcagt gtttctttta gccgtaaaaa ctcctttgct gcctcactct gggcggccaa
    14221 aggagttaac tgttcttcca attcgtggat gatgtcttgg acgcggctga ggttgtcttc
    14281 tgtttcaaaa agcttctgtt ctgctttctt tttccgctgc ttgtatttca agacaccagc
    14341 tgcttcttca aagatcccac gacggtcttc cggtttacta ctgaaaatcg cttctacttt
    14401 tccttgagaa atgattgaga acgattcttt tcctaatcca gaatctaaga acaattcttg
    14461 gatatctttt aatctgcagc tttgtttatt gatgaaaaac tcgctttccc cagtccgtcg
    14521 ataacggcgg gttacactga tctcattgta ctccaatggc agataatgat cactattgtc
    14581 taaaatcact gtgacttctg caatattcag ctgcttacgg gtatcggaac cggcaaagat
    14641 gatatccggc atttttccgc ctcgcagact tttagcagat tgttctccta atacccagcg
    14701 gatagcctct gtaatattgc tttttccgct gccatttggt ccaaccactg ctgtgacgct
    14761 attttcaaaa tcaatgactg tcttgtctgc aaatgattta aaaccggcta tttcaattcg
    14821 tttcaaatac aaagctttcc ctcacttact tttacagtgc tgccaatgca ttttccgccg
    14881 cagcttgttc tgctaatttt ttcgatttcc cttgtccagt gccaatcaat tgatcatcta
    14941 cataaacttc gatccaaaat actcgttcat gagctggacc ttcttcattg atcaatcggt
    15001 attcgattga tacatcacct gatttttgca agacttcttg tagttttgtt ttatgatcca
    15061 tctcatgtga aaaagcacct gctttgactt taggaaaaat cactttttcg atgaatgcat
    15121 gggtcgtgtc gaatccctgg tcaagatata acgcgcctaa aaaagcttcg aataggtcgc
    15181 aaagcaatgc tggtcgcgta cgtcctcctg aattctcttc tcccttgccc aacaaaacat
    15241 attgatcaaa gtgacattct ttggcaaatt tcgacaagct gtcttcgcga acgatcgcag
    15301 ctcgtgtttt tgtcaatttt ccttctggca aatgaggata taaacggaat aaatactgag
    15361 aaaccatcag ttctaaaacg gcatctccta aaaattcaag tcgctcatta tccgataatt
    15421 gcagattgcg atgctcattc acataggatg aatgggtaaa agcttgttca agcaaattta
    15481 gatcgtgaaa aacgatgtta tagttttctt ttagttcttt tgtcagctgg ttgtccattt
    15541 tccacattcc ttttatggat attttttcga tacttcctta ttatactggt tttttgtcaa
    15601 atttgaaatg gttcggtcaa aaggtaagta aagctttaat aatcctagtg attgacgaga
    15661 agttgtgaaa aattcgttta gtttcgagta tgaagaaaaa attttgtgaa aatggctttc
    15721 catatttttg cataaatttg acttaatacc gagaaactgg actttgaaca ccgtttattg
    15781 gagaagtcat gaaatcagcg ccttgacctg aatattagag aaataaacag aaaaatatca
    15841 aaaaaccgaa aaaggacatc gcc
//
LOCUS       DAGCXG010000055        15658 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.058, whole genome shotgun sequence.
ACCESSION   DAGCXG010000055 DAGCXG010000000
VERSION     DAGCXG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.058"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            21..1448
                     /locus_tag="MB022_001744"
     CDS             21..1448
                     /locus_tag="MB022_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466742.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="MB022_001745"
     CDS             complement(1540..1905)
                     /locus_tag="MB022_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBT4466743.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="MB022_001746"
     CDS             complement(1902..2267)
                     /locus_tag="MB022_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBT4466744.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="MB022_001747"
     CDS             complement(2459..2707)
                     /locus_tag="MB022_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466745.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="MB022_001748"
     CDS             2706..2834
                     /locus_tag="MB022_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBT4466746.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="MB022_001749"
     CDS             2891..4585
                     /locus_tag="MB022_001749"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBT4466747.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4589..4708
                     /locus_tag="MB022_001750"
                     /pseudo
     CDS             4589..4708
                     /locus_tag="MB022_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4685..4858
                     /locus_tag="MB022_001751"
     CDS             4685..4858
                     /locus_tag="MB022_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466748.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="MB022_001752"
     CDS             5236..6369
                     /locus_tag="MB022_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBT4466749.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="MB022_001753"
     CDS             6400..6720
                     /locus_tag="MB022_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBT4466750.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="MB022_001754"
     CDS             complement(6749..7213)
                     /locus_tag="MB022_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466751.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="MB022_001755"
     CDS             complement(7345..8598)
                     /locus_tag="MB022_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4466752.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="MB022_001756"
     CDS             complement(8627..8944)
                     /locus_tag="MB022_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4466753.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="MB022_001757"
     CDS             complement(8970..9290)
                     /locus_tag="MB022_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4466754.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="MB022_001758"
     CDS             complement(9754..11169)
                     /locus_tag="MB022_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4466755.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="MB022_001759"
     CDS             complement(11186..12502)
                     /locus_tag="MB022_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4466756.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="MB022_001760"
     CDS             complement(12652..13350)
                     /locus_tag="MB022_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBT4466757.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14595)
                     /locus_tag="MB022_001761"
     CDS             complement(13639..14595)
                     /locus_tag="MB022_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4466758.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="MB022_001762"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="MB022_001762"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4466759.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4864 a         3175 c         2687 g         4932 t
ORIGIN      
        1 tataaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagaga
//
LOCUS       DAGCXG010000056        14995 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGCXG010000056 DAGCXG010000000
VERSION     DAGCXG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.059"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="MB022_001763"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="MB022_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4466760.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="MB022_001764"
     CDS             complement(322..891)
                     /locus_tag="MB022_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBT4466761.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="MB022_001765"
     CDS             1077..1979
                     /locus_tag="MB022_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4466762.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="MB022_001766"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="MB022_001766"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBT4466763.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="MB022_001767"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="MB022_001767"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBT4466764.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="MB022_001768"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="MB022_001768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBT4466765.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="MB022_001769"
     CDS             8547..8732
                     /locus_tag="MB022_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBT4466766.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="MB022_001770"
     CDS             complement(8794..9990)
                     /locus_tag="MB022_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4466767.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="MB022_001771"
     CDS             complement(10160..11596)
                     /locus_tag="MB022_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBT4466768.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="MB022_001772"
     CDS             11889..12698
                     /locus_tag="MB022_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4466769.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14881)
                     /locus_tag="MB022_001773"
     CDS             complement(12884..14881)
                     /locus_tag="MB022_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBT4466770.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3178 c         2661 g         4779 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DAGCXG010000057        14567 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGCXG010000057 DAGCXG010000000
VERSION     DAGCXG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.060"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="MB022_001774"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="MB022_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBT4466771.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="MB022_001775"
     CDS             808..1242
                     /locus_tag="MB022_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBT4466772.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="MB022_001776"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="MB022_001776"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBT4466773.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="MB022_001777"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="MB022_001777"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBT4466774.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="MB022_001778"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="MB022_001778"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBT4466775.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="MB022_001779"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="MB022_001779"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBT4466776.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="MB022_001780"
     CDS             9513..10439
                     /locus_tag="MB022_001780"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBT4466777.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="MB022_001781"
     CDS             10621..11514
                     /locus_tag="MB022_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBT4466778.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="MB022_001782"
     CDS             11648..11773
                     /locus_tag="MB022_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466779.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="MB022_001783"
     CDS             complement(11907..12539)
                     /locus_tag="MB022_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466780.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="MB022_001784"
     CDS             complement(12687..13229)
                     /locus_tag="MB022_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBT4466781.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEKRAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="MB022_001785"
     CDS             13364..13753
                     /locus_tag="MB022_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466782.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="MB022_001786"
     CDS             13757..14521
                     /locus_tag="MB022_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4466783.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
BASE COUNT         5144 a         2475 c         3021 g         3927 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg cttttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat ccgcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gtttttt
//
LOCUS       DAGCXG010000058        14480 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGCXG010000058 DAGCXG010000000
VERSION     DAGCXG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.061"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(286..3960)
                     /gene="rpoC"
                     /locus_tag="MB022_001787"
     CDS             complement(286..3960)
                     /gene="rpoC"
                     /locus_tag="MB022_001787"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBT4466784.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(4018..7635)
                     /gene="rpoB"
                     /locus_tag="MB022_001788"
     CDS             complement(4018..7635)
                     /gene="rpoB"
                     /locus_tag="MB022_001788"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4466785.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8363..9331
                     /locus_tag="MB022_001789"
     CDS             8363..9331
                     /locus_tag="MB022_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4466786.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9349..10254
                     /locus_tag="MB022_001790"
     CDS             9349..10254
                     /locus_tag="MB022_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466787.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10251..11057
                     /locus_tag="MB022_001791"
     CDS             10251..11057
                     /locus_tag="MB022_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4466788.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11193..12182
                     /locus_tag="MB022_001792"
     CDS             11193..12182
                     /locus_tag="MB022_001792"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBT4466789.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12499..13023)
                     /locus_tag="MB022_001793"
     CDS             complement(12499..13023)
                     /locus_tag="MB022_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBT4466790.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13310..>14480
                     /gene="scm"
                     /locus_tag="MB022_001794"
     CDS             13310..>14480
                     /gene="scm"
                     /locus_tag="MB022_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBT4466791.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDST"
BASE COUNT         4110 a         3120 c         2842 g         4408 t
ORIGIN      
        1 tctactggtt ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat
       61 aaccgctcta tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt
      121 gttgattgac ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg
      181 ttgttaagat cctttcccac ttttcttcta gttagacaaa ttattttaga caaaccaaaa
      241 aagaggttgt gacaataatt tgtcacaacc tcttttttaa aatatctatt ttgtttgatt
      301 gttcattgcg tcttctgcag ccatttgcgc ttcaatatct gaaatactgt agacattttc
      361 gcttgctaca ccgacttctt ttggttccat gtcacgataa cgttgcatac cagtaccagc
      421 tgggatgatc ttacctataa taacattttc tttcaaacca agaagaggat ctttcttacc
      481 gcggatcgca gcatccgtca agacacgagt tgtttcttgg aatgatgcag cagataagaa
      541 actatttgtt tctaatgatg cttttgtgat acctagtaag acaggtcgag aagttgcagg
      601 aacgcctcct gagactagcg tattgtagtt ttgatcttta aagtctgcga tgtctaataa
      661 tgtacctggt aagatatctg tatcacctgg atccattaca cgaactttac gcagcatttg
      721 acgaaccatt acttcgatat gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg
      781 ttgtacttca cgaagcaagt agttttcaac agataataca tcgcgaactt gcaacaattg
      841 ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc
      901 tactttcata cgtgctgtat aaggaacagt atacgtacgt gtatctgttt ttcctttgat
      961 tgttacttct ttggtacgtg ttgcaggatc ttctgagata tcgatgacat cccctgttac
     1021 ttctgtgatc actgcttgcc ctttcggatt acgtgcttca aagatttctt ggacacgagg
     1081 aagaccttga gtgatatcgt ctccggcaac cccacctgta tggaatgtac gcatcgtcaa
     1141 ctgagtacca ggttcaccga ttgattgagc ggcgatcgta ccaactgctt ctccgacttc
     1201 aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt
     1261 attacatgtg aagacagaac ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt
     1321 tgcgatgtct tcggaaatca attggtttcc tgcaatgatc aattcatttg tttcaggatg
     1381 acgtacttct ttacgggtgt aacgtcccac taaacgatct tctaatgatt cgatgatttc
     1441 atttccctcg cggatggctt ggatttcaag acctcggtca gtaccgcaat cttcttcacg
     1501 gatgattaca tcttgggcca cgtcaaccag acgacgagtc aagtaacctg aatcggcagt
     1561 cttcaaggct gtatcggtca tccctttacg agccccgtga gttgagataa acatttccaa
     1621 gacggatagt ccttcacgga agttcgaaat gattggcaat tccatgattc gaccattagg
     1681 agcggccatc aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc
     1741 tccagaatca gacatcatga agattggatt tttcgcttcc aaaccttcca tcagtttttg
     1801 ttggatgctg tcttttgcag cattccaaac agcaatgaca cgttcataac gttcgtcatc
     1861 tgtgatcaga ccacgacgga attgttttgt aatagtttca acttgtttat gggcattttc
     1921 aatgatttct tgtttttcat tcagtacact gatgtcagcg atccctactg tgataccggc
     1981 atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca agcatcttag aagtttctgt
     2041 tactttgaat cgtttgaaga cttctgcgat gatgtttcct aagtttttct tcttgaacgg
     2101 taatactaat tcttgctctt tgatatgtgc aggaatatca gtaccagctt caacaaagta
     2161 tttgtccggt gtttggacgg tcaagttgaa gtctgttggt tcattcagat aagggaattc
     2221 aggaggcatg atctcgttga agatgatctt accaactgtt gtgatcatcg tacgttcttt
     2281 ttgccattca gtaaatggtt tttcacctaa tgagttaggg ttcacaccga tacgtgaatg
     2341 taaatgaaca tatccgttgc gccatgcgat aacggcttcg ttttgatctc tgaagaccat
     2401 tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag tagttaccta gaaccatatc
     2461 ttgggaagga gtaacaactg gtttaccatc tttagggttc aagatgtttt gagcagctaa
     2521 catcaacata cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga cagccatttg
     2581 gtctccatcg aaatcggcat tataagcttc acatacaagt ggatgcaaac ggattgcgcg
     2641 tccttgtact aaaactggtt cgaatgcttg gataccaagt cggtgaagcg taggtgcccg
     2701 gttcagcaat actgggtgtt ccttgattac gtcttctaaa acatcccata cttcgtcttc
     2761 ttgacgttcg attttacgtt tcgcattttt gatgtttgaa gctagttcgc gttgaacaag
     2821 ctcacgcatc acgaatggtt tgaataattc aatggccatt tcttttggaa gtccacattg
     2881 gtacatcttc aagaaaggtc caacaacgat aacggaacga cctgagtagt cgacacgttt
     2941 acctagcaag ttttgacgga aacgaccttg tttccctttc aacatatgag aaagagattt
     3001 caatgggcgg ttacctggtc cagtaactgg acgtccgcga cgaccgttat cgatcaatgc
     3061 atctactgct tcttgcagca tacgtttttc attttgaacg atgattccag gtgcattcaa
     3121 gtctaacaaa cgtttcaaac ggttattacg gttgattaca cgacggtaaa gatcattcaa
     3181 gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat ctggtggaat
     3241 aacagggata acgtccataa ccatccagct tggttgattt cctgaagcgc ggaatgcttc
     3301 taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc
     3361 ttctttcaat tcggctgctt ctgcatctag atcaacatta tcaagtaatt gtttaatcgc
     3421 ttctgcaccc atagcagctt ggaattcttg tccatattgt tcacgttttt cgcgatattc
     3481 gcgttctgtc aacaattgtt ttttctctaa attcgtattt cctggttcga tgactacata
     3541 tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc atgtctaata caagacccat
     3601 acgtgaagga atccctttaa agtaccagat atgtgaaaca ggagctgcta attcaatatg
     3661 tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc actccgcatc gatcacaaac
     3721 gattccttta taacggatac gtttgtattt tccgcaggca cattcccagt cttttgtagg
     3781 accaaaaatc cgctcacaga acaacccttc gcgttcaggt tttaaggtac gatagttaat
     3841 cgtctcaggt ttttttactt caccgtaaga ccagcttctg attttttcgg gagaagccaa
     3901 acctatttgc atactttcga atttatttac atcgatcaaa aggggttccc tccatttcat
     3961 ttaatggcaa accgcaagac ggctgatctt cgaccgcctt gcagctcata gaccggatta
     4021 gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt catcttccac
     4081 ttgttcagca gctttctttt ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa
     4141 agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt
     4201 ttcttcgata tccaagacac gcatgtccag acctagtgat tgcaattctt tcaccaatac
     4261 gcggaatgat tccggtacac ctggttttgg aattggttca cccttgacga ttgcttcgta
     4321 tgttttcaca cgtccgacta cgtcatctga tttgtatgtc aagatttctt gtaaagtata
     4381 agccgcaccg taagcttcca gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa
     4441 ttgagcttta cctcccaatg gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc
     4501 atgcaatttg tcatcgacca tgtgagcaag tttgatcatg tacatcacac cgacagacac
     4561 acgtccatca aatggttcac ctgtacgtcc gtcgtacaga acagttttcg catcgctggc
     4621 cataccggct tcacgaactg tttcccatac gtcttcatca cttgcaccat caaatactgg
     4681 cgttgctacg tggataccta actgacgtgc agccataccc aagtgtaatt ccaatacttg
     4741 cccgatgttc atacgtgaag gcacccctaa aggattcagc atgatatcga ttggtgttcc
     4801 atcaggtagg aatggcatat cttcttccgg cataatacgg gatacaaccc ctttatttcc
     4861 gtgacgaccg gccattttat ccccttcatg gattttacgt ttttgaacga tataaacacg
     4921 aactaacatg tttacgcctg gtgacaattc atctccagct tcacgagtaa agattttcac
     4981 atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg
     5041 agctttttcg ccaaagatcg catgcaatag acgttcttct gcagataatt ctgttacccc
     5101 ttttggcgtg actttcccta caagcaaatc gccatcttgg acttctgcac caatacggat
     5161 gatacccatt tcgtctaaat cttttaatgc gtcttccccg acattaggga tttcgcgtgt
     5221 gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt caatatggat
     5281 agaagtgtat acgtcatctt ttaccaaacg acggctcatg atgatcgcat cttcatagtt
     5341 gtacccttcc caagtcatga aaccaactaa aacgttttgg cctagagcca tttcgccttg
     5401 ttccatagat ggtccgtctg ccagagtatc ccctttttcg actttctcac ccaaatgaac
     5461 gattggacgt tggttgtagc ttgttccgga gtttgaacga cggaattttg ttacatcgta
     5521 tttatctaat gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa cgtattcaac
     5581 gacaccgtca tgtttacata acaaagcagc tcctgagtca tgcgctgatt tgtattccat
     5641 accagtacca acccatggtg cttgaggatt gatcaacggc actgcttgac gctgcatgtt
     5701 ggcacccatc aaggcacggt tggagtcatc gttttccaag aaaggaatac atgctgtcgc
     5761 aacggcaact acttgtttag gtgatacgtc catgtagtct actttatcaa tagatacttc
     5821 taagttttca ctttgagcac gagccataac aacgtcttga gcaaatgttc catcttcatt
     5881 caatgggctg tttgcttggg ctacgatgta atggtcttcg atatcagctg tcaagtagtc
     5941 gatttggtca gtaacacgac ctgtttcacg gtctacacga cgataaggtg tttcgataaa
     6001 tccgaattta tttactttcg cataactgga caaactattg atcaacccga tgtttgggcc
     6061 ttcaggagtt tcgatcggac acatacggcc atagtgggag tagtgaacgt cacgaacttc
     6121 ataaccggcg cggtcacgag tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg
     6181 tgtcaattcg ccaagagggt ttgtttgatc catgaactgt gacaactgag aagaaccaaa
     6241 gaattctttg atacttgcca caactggacg gatattgatc aattgttgag gtgtcaatgt
     6301 ttcagtatct tggatcgaca ttctttcacg aaccacacgt tccatacgtg ctagcccgat
     6361 acggaattgg ttttgtaaaa gttcaccgac tgaacggata cgacgatttc ctaagtggtc
     6421 gatatcatcg acattgccga taccttccat caagttcaag aaatagctca ttgaagcaat
     6481 gatgtcggct ggacgaaccg tttttacagc tgcttctgga tagccgtttc cgatcacgtt
     6541 tacttcacgt tcagggtctc ttggtgaaaa tactttgatg acttggacag tcattggatc
     6601 tgtgaccaca ccgtcttctg atggatagta agtcactgaa tttaggccat tatcaatgaa
     6661 aggagctagt gtttccatga cttggtgagt cagaactgtt cctttttcaa cgatgatctc
     6721 gcctgtttct ggatcaacta atgtttcagc taatgtcaga ttcaatagac gtgttttcaa
     6781 atctaatttt ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac gttttggatc
     6841 gaagaaacga gcattcagca agttacgtga gctgtctgct gtttttggtt cgcctggacg
     6901 caaacgttcg taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg
     6961 caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt
     7021 atcatcggaa ccaaatccta acgcacgaac taatacggtt aaaggaatct tacgtgtacg
     7081 gtcgatacgt acataagaga tgtcttttgc atcagtttcc atttctagcc aagctccacg
     7141 gttagggata acagtagaac cgaatccttc ttttccgttt ttgtcgactt ttccatggaa
     7201 atagacacct ggtgaacgga ctaactgaga aacgattact cgctctgccc cgttgatgat
     7261 gaatgttcct tgttctgtca ttaatgggaa atcaccgaag aatacttctt gtgctttgat
     7321 ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc
     7381 atcatgcgca cgagcttctg ctactgtata ctttggttct ttcaattcat aatctacaaa
     7441 ttccaaagat agattgccat tgaagtcatc gattggcaaa atgtcttcaa acatttctct
     7501 aagtccctca tctaagaacc attggtaaga gtctgtttga atttcgatca aatttggtaa
     7561 ttccaacact tcactgattc ttgcgaaact tctacgttcg cgatgttttc cgtattttac
     7621 tacgtgtcca gccaagctct tcacccctct aaaatgtcat tcaaaaagac tacccgatca
     7681 attgttacaa taatatttca aatattattt ttaggtaaca acagacattt tttgcggctt
     7741 ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg tgagcttcta
     7801 cttttgtttc cttatattaa agccgaaacg gacaatattt cgtttctgcc ttttcttcat
     7861 cagatagttt ttgcaacttc atatactaca cttattttct ataaaagtca acacttcctg
     7921 gcgtttcttc tgctttttcg caacattttc ttaatgtttt caattttatg ttttcaatta
     7981 tctgacaatt cttaattgac agaccttttt atctcggtta tactttagaa aattcatttt
     8041 gtggaggaac ttctatgcga accaacatca accaaatcgc accaacacaa ctgaataact
     8101 tttactttag ctactttaga taggtttgta ttcatcatga tggatacaaa cgttaaacga
     8161 cagtttgtat ctatgatggc taaagaactt tgtattgcag caaaaagcca cgtagacaaa
     8221 atgctaagtg gctgagcagt gaaaatacaa agtaaaagta tcaagcattc tactttttca
     8281 atttattttc cgacttttca atccatttgg acattttgtc tgaatggatt ttttgtttcc
     8341 caaaattaag gaggaaaaag aaatgaaaaa aaatataatc atcggattag gcgctgccct
     8401 gctactagct ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat cagatggaca
     8461 aaaaacaatt ggcgtattgc aacttgtaca acataactcg ttagatgctg cctataaagg
     8521 attcaaagaa ggattagcag aagaagggta taaagaaggc gataatctaa caatcgatta
     8581 tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga
     8641 agatcctgat ttgctacttg gaatcgccac tccagcagca caatctttat taaatgaaac
     8701 aaccactatc ccgatgactg tcacagcggt cacagacttg gaacaagccc atttagtcac
     8761 atccaatgaa aaacctggcg gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa
     8821 acaaatcggc ttactgttaa atatcgtgcc agacgccaaa acaatcggca tcatgtataa
     8881 caacggtgaa gcaaactcta agatccaagc tgatttggca gaaaaagaat tgaaaaaagc
     8941 gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa
     9001 aagtctagct aaagatgtag atggaatcta tattcctact gataacacgt tcgcaagtgc
     9061 ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt
     9121 tgatcaagtc aaagacggcg gtctagcaac agtaggaatc gactacgaaa gcttgggtaa
     9181 acaaacaggg atcatggcag caaaaatctt agacggcaag aatccaagtg aacttcctgt
     9241 cgaaacagcg aatgatttgg aactgtatgt caataaagac atggccaaag cattaggcat
     9301 caatccagac agcatcaaag aagctgaata aaacacagga ggattctcat gacaaatttg
     9361 ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat gggccctact tgcgattggt
     9421 gtatacgtca ctttccgtat cttggacatt gccgacttga ctgctgaagg aagctttcct
     9481 ttaggtgcag ggatcgcggc agtaagtatt acaaatggct atcatccatt gacagcttgt
     9541 cttctaggct ttttaggggg tgcggcagca gggcttgttt ccggactgct tcacacaaaa
     9601 ttgaaaattc ccgcccttct tgcagggatc atcaccatga ctggtttata ttctgtcaca
     9661 tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc agccaacgat ctttcattgg
     9721 gtcgagtcat tcggcataac aaaagacaac gctgtactga tcgtcggtct gctttttgct
     9781 ttgatcgttg tatgtttact tgttttgttc ttacgtacag aaacaggact tgctattcgt
     9841 gcaactggcg ataatctggc aatgagcgaa gcaaacggga tcaacacaga taatatgaag
     9901 atcatcggtt atatgatttc taatggatgc attgctcttt ctggctcctt gcttgcgcaa
     9961 aacaacggct ttgctgattt aaattctggt attggaacga tcgtaattgg acttgcttct
    10021 attatcatcg cagaagtttt gttacgtaac cagccactat ttctccgtct cttgactgtc
    10081 gttttaggtg cggtcattta tcgttttatt ttagcgttag tcttcgaact gaatgttgaa
    10141 ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga tctgtctttc tttcccgcag
    10201 atccagcaaa aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg
    10261 tcttaacatt ggaaaatctc catcatacct ttgaaaaagg aacgatcaac gaaaatcatg
    10321 tcctgaaagg cattaatctt tccttagatc ccggcgactt cgtgacgatc atcggtggaa
    10381 atggggctgg taaatctact ttgctgaaca gcatcgccgg tacattttct gtcgatcagg
    10441 gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat
    10501 ccatcagtcg cgtattccaa gatcctaaac tagggacagc cgttcgttta acggtagaag
    10561 aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac
    10621 cacaagaccg aacatttttc aaagaccagc tggttcggct aaatttgggg ttggaaaatc
    10681 gtttatcctc agaaatcggt ttgctgtctg gaggacaacg tcaagctatt acgttattga
    10741 tggccacatt acagcgtcca gaactgatct tattagatga acacactgca gcgcttgacc
    10801 ctgctacttc caaaacggtg atggaattga cagaaaagct gatcactgag cagaatctca
    10861 ctgcttttat ggtcactcat gacatggaag acgcgatccg ctatgggagt cgactgatca
    10921 tgctgcatca aggaaggatc gtcgtagata tccagcaaga ggaaaaacag cagctcacag
    10981 tgccccaatt attagcgttg ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag
    11041 tacttttatc ttcataatca gaaagaggat gggacaaaaa atgaacgccc accctcttct
    11101 tttttctctt tccttctttc gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact
    11161 attttgttct ctccttcttc ctgatagaat ggatgggaaa ggagcttgat gccatgacaa
    11221 cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga ttttctttcc ggagaaaagt
    11281 tggcacagca actggaagtt tcccggacaa gtatttggaa agccatcaaa gaattggaaa
    11341 aggcaggcta tcgttttgag cacgggccta cagggtatcg ttacctgccc tctgacgtac
    11401 tagatgcaaa agaaatccag caagagctac ggtcgtttat tcctgatttg aaagtacaaa
    11461 cgatgttgac ttctgaatct acgatgaaag atgccaaact ggcagcagct caaggagatt
    11521 ctgtttcgaa gttatttgtt gctgatacac aagaacaagc tcgtggacgt tttggacggc
    11581 cttttttcgc tgaacctgga cgaggaatct acatgagtct cttgctccat ccagacaaac
    11641 ggtttgatga attgcctcaa tatacgatca tcgcagccag tgcctgtgtg aaagccattg
    11701 aatctgtaac cggtcgttct gcttcgatca aatgggtcaa tgatatctac atggatggaa
    11761 aaaaagtctg tgggatttta tctgaagcag tcagtgatat ggaaagcggc aggatcagtc
    11821 atatcatcat tgggatcggg cttaattttt ctgttcctca aaatcatttt ccagctgatc
    11881 tccaagaaaa agccatctct gtctttcccg acggacaagc tagtgtcaca aggaatcagc
    11941 taatcagcaa aatctggcaa gaattgttca cttatctttc tcacccagaa aaagcgttgg
    12001 acttctataa agaaaaatca ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa
    12061 cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa
    12121 atgaacagaa gatgagtttg tcttctggcg aaatcagtct cacatctatc cgtacgcaat
    12181 aattatcaga tttcacaaaa ggacacctga tctcatacag tgtccttttt tctgaattct
    12241 cttcaaaaga ctctccttat tctatcagct gacagtttct ctatcctcta gaaaaaggta
    12301 ttctaaaaat atctataaag catggtacac tttctttaaa tgaatcaatg caaaaaacaa
    12361 gagagtcatc agtttacacg atcaaaagag atagcaggcc gcttcctttt tcaatcttat
    12421 agatagacca tgggtaacga taaaaacaaa aaagaacttg cgacttattt tgccgtaagt
    12481 tcttttttag ctagtgcctt aatctcttaa tcccaacaaa cgtctcgcat agatcacctg
    12541 accagagtgc atagcagcat catcaataat agaaacgaga cgcattcctc gtgtgactgg
    12601 tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa ggttccagcc ttcttaagta
    12661 agaaatagca aaatcagcag ctcctcgcaa gtatcctagc agatcttcta tttgattagc
    12721 ttggatctgc tttgcttgtt ccagactatg cttccaatct tgttcgttat ctgctatcgt
    12781 catcggaaac cggctttccc agtcttgact atgccaaatt ggagaagttc ctgctaaatc
    12841 agcaatctgg aaatctaatt ctcgagcggt atgccaagcc agccaagtca ttgatttgat
    12901 ctgcggggct ctatcttcca ctggaaaacg attggcctcc tcaatagaga cgccttcaaa
    12961 caaacgttca aaccgatcgt atgcccgatc cacactctcg catagcatat ctaaataatc
    13021 catttatttt cctccttttt atttatccta cttacttttt cctaattcta ggatacctac
    13081 ttatatgtta ctacacaccc ttatttttta cactttaaca gtcaccatca tcaatagttt
    13141 ggagattgaa agaaaaagat agtgtcgaac tatcaattga acaagaagca acaatatgaa
    13201 cgtttcatta atattcttta aataaacaat gatttttcaa acgaacagtt taagactagg
    13261 agaaaaagag tataatagga aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag
    13321 ggcaagttta ctagtcctag ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt
    13381 ttcagcggaa acatacattc ctaaagaccc tgggaatatt tataatgatc taactggtaa
    13441 ctatggcttg ttaggaattg ctagtcaatt ccacgtattt gctaaaaata agacaactgt
    13501 gaatgcacat acagacggaa atatagcaac acatgaactt gatgcaaaaa ataattttgg
    13561 tacagagctt catgaaggtt tattgaacca agaaataaat tatatacaaa aaactgattc
    13621 tttcgctaat tcttcaggca tacctacagg tagcacaaga atgaataaat ttgtcgttgg
    13681 cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca gtcaatggat ccaggcttga
    13741 tcacttaact ccagatgatt tttataaaga tcgtgctggg catgaatata ttgatttcga
    13801 gcaggaattt gctaaattga atgtagcagc aaatgatctt gcaacgatca ctccagctaa
    13861 aacatatact gctgcagatt ttccagacat gaacaatcgg acaatcgatt taactgattt
    13921 atcagatacc gggtttttat tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc
    13981 acttcaaatc attaatccaa atgatcaagt agttgtattt aatgtcatca acagttcttc
    14041 tgcattgaac gtacagtcac caatcaaata caatgatcgt tcaaatcatg aaacagaaga
    14101 tttttcggat gcgaatatct cttggaattt tggaaatgag atgactgatt tgacaatcag
    14161 tgctcctttt caaggaacaa tccttgcgcc taatgctact atccgcatta ctcaaaacca
    14221 agacggaacg attataggaa caaatgtgat tttagatgca gctaccaatc gttgggatcc
    14281 taacgaaata tttatcagcg acaatggtac agatacgaca gacacaagcg acagtacaga
    14341 tacatctacc acaagtgata gctcagattc atcaacttca agcgacagta cagatactac
    14401 ctctaccaca agtgatagca cggatacatc agcttcaagc gacagtacag atactacctc
    14461 taccacaagt gatagcacgg
//
LOCUS       DAGCXG010000059        14376 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGCXG010000059 DAGCXG010000000
VERSION     DAGCXG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.062"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            142..523
                     /locus_tag="MB022_001795"
                     /pseudo
     CDS             142..523
                     /locus_tag="MB022_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            654..1604
                     /locus_tag="MB022_001796"
     CDS             654..1604
                     /locus_tag="MB022_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4466792.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2122..2577
                     /locus_tag="MB022_001797"
     CDS             2122..2577
                     /locus_tag="MB022_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4466793.1"
                     /translation="MVKQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2918..3520
                     /locus_tag="MB022_001798"
     CDS             2918..3520
                     /locus_tag="MB022_001798"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466794.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3614..3946
                     /locus_tag="MB022_001799"
     CDS             3614..3946
                     /locus_tag="MB022_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HBT4466795.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4097..5076)
                     /locus_tag="MB022_001800"
                     /pseudo
     CDS             complement(4097..5076)
                     /locus_tag="MB022_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5081..5848)
                     /locus_tag="MB022_001801"
     CDS             complement(5081..5848)
                     /locus_tag="MB022_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4466796.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6338..6763
                     /gene="lacA"
                     /locus_tag="MB022_001802"
     CDS             6338..6763
                     /gene="lacA"
                     /locus_tag="MB022_001802"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBT4466797.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6780..7295
                     /gene="lacB"
                     /locus_tag="MB022_001803"
     CDS             6780..7295
                     /gene="lacB"
                     /locus_tag="MB022_001803"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBT4466798.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7306..8238
                     /gene="lacC"
                     /locus_tag="MB022_001804"
     CDS             7306..8238
                     /gene="lacC"
                     /locus_tag="MB022_001804"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBT4466799.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8241..9221
                     /gene="lacD"
                     /locus_tag="MB022_001805"
     CDS             8241..9221
                     /gene="lacD"
                     /locus_tag="MB022_001805"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4466800.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9249..9566
                     /locus_tag="MB022_001806"
     CDS             9249..9566
                     /locus_tag="MB022_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4466801.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9566..11272
                     /locus_tag="MB022_001807"
     CDS             9566..11272
                     /locus_tag="MB022_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4466802.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11414..12820
                     /gene="lacG"
                     /locus_tag="MB022_001808"
     CDS             11414..12820
                     /gene="lacG"
                     /locus_tag="MB022_001808"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBT4466803.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12843..13115
                     /locus_tag="MB022_001809"
     CDS             12843..13115
                     /locus_tag="MB022_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HBT4466804.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13136..14035
                     /locus_tag="MB022_001810"
     CDS             13136..14035
                     /locus_tag="MB022_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBT4466805.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4696 a         2371 c         2771 g         4538 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgatgacact atcgttcttc atcaacgtaa
       61 gttagatttg ttgaaagagc agaaaaaagg atttctacaa aaaatgtttg tttgatattt
      121 taaatagaaa agagagataa agtgaagttt ttaaattttt tgaactataa atatttaaac
      181 tgggtattat atggtattac attatttttt attatttatt acttttttac cgatgatgat
      241 atttttttaa ttttagcaat actttttctt gtttcatcta attttagtga taacttaatt
      301 gaagttctta cagaaccaga aattcggtct atgacggatg aagaacaaca gattattaaa
      361 gctatgattt cggaaaataa gaaagacgta gtagttatca aaaagattag agagtataca
      421 aatttaggtg ttttacaagc caaaaaagta tatgaaaaaa aaaaaaaggg tctataatta
      481 caaattagat aatacattag aagctggaga caagattgtc tagataatgt gtgagataaa
      541 atttttggca ggttatcata cataagacaa aatgttttta aaactgataa cgtatttatt
      601 ccacaacaat tacgcattta tttctgagaa aaatataaag caggaggatt acaatgctga
      661 aaacttattc cttaacaaaa gatacattat tagtttcgtc agaatcaaat atcaaaaatt
      721 cttctgttgt ttatgtttat ggtgacgaac gagaatatat cgaatcttct gaacataaat
      781 atgactttaa aataggcaat atactaacgt ttgatgatcg agttgcatat gacacgatga
      841 tagataagaa cgatgaaaaa agtttgctcg taagtttttt gtttcccgag gtacatgaag
      901 gtgggcatct agataccctg acgacatctg ttgttttctt ggtatttaaa aataagttga
      961 taatttttac aaaacaaaaa ttaaaattca ttcccgaact cttatccaat atggttttac
     1021 gtgatgtcaa tcatttcgtg gaggaagtac ttttaaagat catgcaaaaa gatttctata
     1081 tgatgcttaa cgaccttcgt ggagtcaagg aaaaaattga tgatttagac tcagaaatat
     1141 caacgtccgg ttcactcaaa ccaacgtttg gtgatttatt gactcttcaa aaatacatga
     1201 tagctttgtc aacaacttat aaagctaacc ataaagcgct tgatttcatt aaaaaaaatt
     1261 tcacaactat tgacgatatt gattttagta ctaaaaaaat aattgatgat ttatacgata
     1321 caagtgatac aatggataga atcattttaa gttacagcca atatttggac aaattggaaa
     1381 atatgataaa caatatgatt tcgtatcagc taaacatgat tatgaaaacc ctaaccgaga
     1441 tatcaattgt tttgactatt ccagcaatta tttttggctt ttggggaatc aatgtccatg
     1501 tcccgtttga aaagtcttct tatggtgttt tagccgtatt gattatctct gctatactaa
     1561 gtctaatttg ctggcttttg atgcgacgca aaacatattt gtaaagtatt tgtgataccc
     1621 taatatgatt taaagcagaa tttctttgtt gactttttaa aaggctgatt cctttcaata
     1681 atttaatagc ttcagcggct gctcagaagt caataaattg gactattttt attcagttac
     1741 ttaggcaaca tttgagatac gattgcgatt ataaaaattg atataccaac caatggctgc
     1801 ttggaagact gacatattag cctcgatatc atcgaataac taaatacaga aaaatacaat
     1861 aaatattatc aaactaggga ctatactaga aaacatcagt acattcctta actcagggtc
     1921 tataattaga aaatcgcccc tttgaaaact gattaaaaca gcccccatta tctagtgact
     1981 agataatggg ggctgttttt ttgttaagtg atataataaa ccataaaaga agtttagaaa
     2041 tacggtcctg gtcatcaata aataaacaat ttaattaatt atttatttaa ttatttaatt
     2101 tcagaaaggt atacatgaat catggtgaaa caaattgtcc tacccatcaa agactccagc
     2161 gtcttaaaga tggtacaaga tacacttctc gatagtttcc gtgctggtcg ccgaaattac
     2221 accgtttttc aagttggtaa ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa
     2281 aaatcagatg tctataatcc agacggctct gtcaaaaatg caacctttat tcatgataaa
     2341 aagaccggta aagcaaacac gttatattta aagcctgttc agcaagactt gttgcaatat
     2401 catgattggc tggcccaaga aaatatcaat tctgagtggt tatttccctc gacagcccat
     2461 tatgatcttc atatcactga gaagcagttt cataaggtga tggcgcatgt tggtgatctc
     2521 ttaggaatca acttggaaga caaagagaaa aaattgcgag tcagaaaacc gttttgagat
     2581 atgaacagca tttgccttca cgccttagcg attttaaata atagccttga aagtgattat
     2641 ggaaaagccc tctacgctca atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa
     2701 ggtgtatttg gtatgtgaag aacccatgtc agaggcaaga acgttgcacc tactgacatt
     2761 ggaatgatgc ttatgagcat gaatctaacc aaattatccc ttgaggctag aagaaaagcg
     2821 gaagcttttc aacacaaagc agccaaaaat aaaaaacgcg acgaagcaat cacatttatg
     2881 attattcatc gcgtttttta tatttagagc tagttatttg ctagacccac tatttacaga
     2941 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     3001 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     3061 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     3121 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     3181 gattatccat aatgcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     3241 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     3301 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     3361 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     3421 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3481 ttttaaaaaa gaaccttttt tcgaggtgga agctaagtaa aacaagggtc atttaagggc
     3541 gttctaagcc ccacacagcg ttttaaaacc caagaggagc tttttagctt ttgtttcttc
     3601 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     3661 gaaaacgaat agtccgagtt cgttttcttt aagtagtttg caatcaaaag tcaatcagct
     3721 ttataaagcg acagcgagcc aaactttaaa agctatgcaa ggactgtatg aagcaaaatt
     3781 attgagttat ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa
     3841 agcgaatttt ggcaaatata gcggtttttt aggacttgat cttgaaatgt tgttatcatt
     3901 gataatgata cgttcaaaca acagcaccca aattttgacg aattagtgaa actctatgaa
     3961 aaagacgtag taaaacatgc aacatcctat tctaatcagc tggtaaagct taattaggtt
     4021 atataagctt gaaaataaag ggtttgtcca atatggacaa gcctttttct tatgttttgc
     4081 cacagatttg ccacacttat tgtgtaagag tatcaaggat aggtataatt tgatactttg
     4141 tttttttagt gatgtgcaaa taaatttctg ttacacgact acctctagaa tgccctacac
     4201 ggtcttgaat tgcttctagg ggtattcctt actctgctaa gaaactaata tgcgtatgac
     4261 ggaatatatg ggtcgtaata tgtttattaa aactaagttt attatcaata tcaattcctg
     4321 ctcgttttgg attacctttt cgatttaatt ttccattttc aggtgagttg aggaaattgt
     4381 taaggacact ataatgaaga ggtgttccat tatagtcggg tttatgacga cttttggaag
     4441 taaaaatcca tccattatct ttataatcag gattgaatct catatgttgg tgatttaact
     4501 caatttcctc tttaagaatt tctatagccc ttttagataa ttggacattt cggtaacttt
     4561 gtaagttttt tacattacct tcatagctat tttctgcagc tcctccgtga gtggactgac
     4621 taatgatgat attttgcttt tcaaaatatt gataggcacc aatattttgg agtttcgact
     4681 ctgtaagtca acagggccat tagttagaat acctattggg atgcggtttt tatgtagagc
     4741 cagcagtagt tgatgtatct caggaatcat cgtaatctga tcgagttcat attcatagtc
     4801 agcttggaag tctagagctt ctgtcctaga aatactatcg atgccaaatt cttccatagt
     4861 cttctgacag cgaaaaattc taagctgctc aatactgatg agttttttcg tgtatttagg
     4921 aaaagcaaca tcagaccaat atctgaaacg tttgtaaatg ttatctattt gttggatatc
     4981 aatcgttgga aaacatttct caacgctacg tttaaagggg agttgatggt cataaagagt
     5041 gtcatcaagg tcaaaaacaa tatttttata agtcataatt ctagtccgct tttaaaatag
     5101 tagtgtactg gctaaattcc tctaagtgtt gagggctaat ctggttatct gtaatgatgg
     5161 tatcgagttg atctagatta tagaagttaa agaaatcgta tcgatcgaat ttagtactgt
     5221 ctactaataa gaatttttct acagcattgt ttagggcaag ttgttgaatc acaccttcct
     5281 tgtcactata tgtagcaata gaattatggg taacagcatt tgcactaaca aaagctttgg
     5341 caaatctaat tgcttttaaa tttgtcgaag ccattgaacc tacaaaagct ccagttattt
     5401 ctctatattc accgccaaga agcaataaat caatggtttc gctatcattt agaatcaaga
     5461 acacagggag actatttgta atgacacgaa ttttataacc tttacgacct ttcaattcta
     5521 atgccagttg ttcaagtgta gttccaggtc caataaaaat agtatcgcca tctttgatta
     5581 aagagcaggc ttttttagca atgtcaattt tttcttttgt atttagactt ttcttctcga
     5641 tatgtgtctt ttcaagtggc tttttagagg aaagcaattg tgcaccacca tgagtacgtg
     5701 ttaaaagtcc atcagcttct agagcatcaa ggtctctacg ggctgtcata tcggaaatat
     5761 ctagttcatc tattatttct tttatggtta tggtcccatc aatctttaac atatctaaaa
     5821 ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt ctttcataaa gtaatttttt
     5881 tccaaagata attctctttt aattgtatca taaaagacaa tattttcaag gtaaaacaaa
     5941 caatttcaaa caaaaacaaa tgttagatga tgaaataaga acagaggatt gacgtatatt
     6001 agcttaggtc agattttgta taagacgaaa ataaagtagg acctcttaat cagtaagtta
     6061 tagaaagtaa aagacttttg taatacttga atagatattt cacgtccatt ttgtgatgga
     6121 ttaaatgaac aaaaatgaac aataatttaa cggtgttatc tattttttaa aaaaacaaat
     6181 aaaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg tttgaatgct tgatattata
     6241 taaacaaaga aatgatgaaa acgttttttc gaacattttg caaaatgttt tctacttcta
     6301 cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg gctattgttg ttggtgcaga
     6361 tctcaaaggt acgagattaa aagatgttgt gaaaaatttt cttgtagaag aaggttttga
     6421 ggtaattgat gtgactaagg acggacaaga ttttgttgat gttaccttgg cagttgcctc
     6481 tgaagtaaat aaagatgagc aaaacttagg tattgtaatt gatgcttatg gagctggtcc
     6541 attcatggta gcaactaaaa ttaaaggtat ggttgcagca gaagtttcag atgaacgctc
     6601 agcttatatg acacgtggac ataacaattc acgaatgatt acggttggcg ctgaaatcgt
     6661 cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc aatggtaaat atgatggcgg
     6721 acgtcatcaa atccgagtag atatgcttaa caaaatgtgt taagaaaagg agttaaggaa
     6781 tgagaatcgc aattggatgt gaccacattg tcacagatga aaaaatggca gtatcagaat
     6841 tcttgaaatc aaaaggacat gaggtccttg atttcgggac atatgaccac gcacgtactc
     6901 actaccctat ctatggtaaa aaagtcggtg aagcagtagt aagtggtcaa gcagacttgg
     6961 gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc agtcaataaa gttccaggtg
     7021 ttcgctcagc gttggttcgt gatatgacat cagctatata tgcaaaagaa gagttaaatg
     7081 cgaatgttat cggtttcggt ggaaagatta ctggtggtct tcttatgaat gacatcattg
     7141 aagctttcat tgaagctgag tacaaaccaa cagaagaaaa taaaaaattg attgcaaaaa
     7201 tcgaacacgt tgaaacacat aatgcacatc aagcagatga agatttcttt acagaattcc
     7261 ttgaaagatg ggaccgtggt gactaccacg attaaggtat aacaaatgat tctgacagtc
     7321 acactaaatc cttcagtaga catctcttat cctctagaaa cattgaaaat tgatacagtg
     7381 aatagggtga aagatgttag taagacagct ggtggaaaag gcttaaatgt tactagagtc
     7441 ttgtatgaat ctggtgataa agttactgct acaggtttct taggtggtaa aattggtgaa
     7501 tttattgaaa gcgaattaga acaatctcct gttagtccag ctttttataa aatctcagga
     7561 aacactagaa actgtattgc tatcttacac gagggtaacc aaacagaaat tttggaacaa
     7621 ggaccaacaa tttctcatga agaagcagaa ggctttcttg accactatag caatcttatt
     7681 aaacaatcag aagttgtgac aatttcagga agtttacctt caggacttcc caacgattat
     7741 tatgaaaaac ttatccaact tgcttcggat gaaggagtag cagtcgtttt ggattgttca
     7801 ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac ctactgccat caaacctaat
     7861 aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa aagatattga agaactcaaa
     7921 gacgttctta aagagtcact ttttagtggt atcgaatgga tagtggtctc attaggtcga
     7981 aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg tagatattcc tgatatccca
     8041 gttgttaatc cagtcggatc aggggactca acggtagctg gtattgcatc agctttaaat
     8101 agtaaaaaaa gtgacgctga cttattaaaa catgcgatga cattgggtat gttaaatgct
     8161 caagaaacaa tgacagggca tgtgaatatg actaattacg aaacactaaa ctcacaaatt
     8221 ggagtaaaag aggtataaaa atggtactta cagaacagaa acgcaaatct ttggaaaaaa
     8281 tttcagataa aaacggtgtt atctcagctt tggcatttga ccaacgtggt gctttgaaac
     8341 gtttgatggc acagtatcaa gatacagaac caactgtagc tcaaatggaa gaactaaagg
     8401 ttttggttgc tgatgaactt acaaaatacg cttcttcaat gttgcttgac cctgaatatg
     8461 gacttccagc aacaaaagca ttggataaag aagcaggtct tcttcttgcc tatgaaaaaa
     8521 caggttatga tacatcaagt acaaaacgtt tgcctgactg cttggatgtt tggtctgcaa
     8581 aacgtatcaa agagcaaggc gctgatgccg ttaagttctt gctttactat gatgtagaca
     8641 gctcagatga acttaaccaa caaaaacaag cttatattga acgtgttggt tctgaatgtg
     8701 tcgctgaaga tattcctttc ttcttggaaa tccttgctta tgatgaagaa atctcagatg
     8761 caggttctgt tgaatatgca aaagtgaaac ctcgcaaagt cattgaagcg atgaaagttt
     8821 tctctgatcc acgctttaac attgatgttc ttaaagtaga agtaccagtt aacgttaaat
     8881 atgtcgaagg ttttgctgat ggtgaagtag tttacagtaa agctgaagct gctgacttct
     8941 ttaaagcaca agaagaagcg actaaccttc catacatcta cctaagtgca ggtgtatctg
     9001 ctaaattgtt ccaagaaaca ttgcaatttg ctcacgactc aggtgccaag tttaatggtg
     9061 tgctttgtgg acgtgctact tgggcaggat ctgtagaacc ttacatcaaa gaaggtgaaa
     9121 aagcagcacg tgaatggttg cgtactactg gattcgaaaa tattgatgaa cttaacaaag
     9181 ttcttgttaa aacagctagt ccatggactg ataaagtata gtaaaaacat aaaacggagg
     9241 atattgttat gaacagagaa gagatgactc tcttagggtt tgaaattgtt gcttatgctg
     9301 gagatgctcg ctctaaactt ttagaagcgc ttaaagcggc tgaaaatggt gatttcgcta
     9361 aggcagatag tcttgtagta gaagcaggaa gctgtattgc agaggctcac aattctcaga
     9421 cagctatgtt ggctcgagaa gcttctgggg aggaacttcc atacagcgtt accatgatgc
     9481 atggtcagga ccacttaatg actactgtct tattaaaaga tgtgattcat cacctcatcg
     9541 aactttataa aagaggagca aaataatgca taaactcatt gaacttattg agaaagggaa
     9601 accgttcttt gagaaaatct ctcgaaacat ctatcttcgt gctattcgtg atggatttat
     9661 cgctggtatg ccagtcatcc ttttctcatc tatctttatc cttattgcct atgtcccaaa
     9721 tgcttgggga ttccactggt caaaagacat tgagaccttc ttgatgactc cttatagcta
     9781 ttcgatgggt atcctagcat tctttgttgg tggtactacc gctaaagctt tgacggactc
     9841 aatgaaccgt gacctacctg cgaccaatca gattaacttc ttatctacga tgctagcgtc
     9901 tatggtaggg ttcctcttga tggcagctga gcctgcaaaa gaaggaggct tcttgacagc
     9961 cttcacggga acaaaaggtc ttttgacagc atttattgca gcctttgtga cagttaatgt
    10021 ttacaaggtc tgtgtgaaaa ataatgttac tattcgtatg cctgaagagg ttccaccaaa
    10081 tatctctcaa gtatttaaag acttgattcc attcactgta gcggttgttc tcctttatgg
    10141 atttgaactc attgttaagg gaactcttgg tgtaaccgtt gcagaatcaa tcggtaccct
    10201 tcttgctcct ctcttctcag ctgcagatgg ttaccttgga attacactta tctttggtgc
    10261 ttatgccttc ttctggtttg ttggtatcca cggtccttca attgtcgaac cagcaatcgc
    10321 tgctatcact tatgccaata tcgatgccaa cttgcatctt atccaagctg gacaacatgc
    10381 agacaaagtt atcacttctg gtactcaaat gtttattgtt accatgggtg gaacaggagc
    10441 tacattgatt gttccattct tgttcatgtg gatttgtaaa tcagaacgta accgtgccat
    10501 cggacgtgcc tcagttgttc caacattctt tggggttaat gagccaatct tatttggtgc
    10561 accaatcgta ttgaatccga ttttctttgt accgttcatt ttcgctccga ttgtcaacgt
    10621 ttggatcttt aaattctttg ttgacacatt gaacatgaac tcattctctg ccaaccttcc
    10681 atgggttact cctggtccgt tgggaattgt actcggtact aacttccaag ttctatcatt
    10741 tatcttagcc ggactcttgg tagttgttga tactatcatt tattacccat ttgttaaggt
    10801 atacgatgaa caaattcttg aagaagaacg ttctggtaaa acaaacgatg ctcttaaaga
    10861 aaaagtagct gcaaacttca atactgctaa agcagatgcc gttcttggaa aagcaggcgt
    10921 tgctaaagaa gatgtagcag caaacaataa tatcacaaaa gaaactaatg tccttgttct
    10981 ttgtgcaggt ggaggtacaa gtggcttgct tgccaatgcc ttgaacaaag cagcagcaga
    11041 atacaatgtt ccagtcaaag cggcagcggg tagttacggt gctcaccgtg aaatgttgcc
    11101 agagtttgac ttggttatct tggctccaca ggtcgcttca aacttcgatg atatgaaggc
    11161 tgaaacggat aaattgggca ttaaacttgc aaaaactgaa ggagctcaat atatcaaatt
    11221 gacacgtgat ggacaaggtg ctcttgcatt tgttcaacaa cagtttgatt aataaatttg
    11281 taaaagttat ttgagacgag attattcttt caattcctta taatattgtt aaccatatca
    11341 ttgcatttca tttccttggt ttccggaact cagaatatcg tctcaaatgc tttttttgaa
    11401 aggatttata gttatgacta aaacacttcc taaagatttt atttttggtg gtgctacagc
    11461 tgcttatcaa gcagaaggtg cgactcacac ggatggtaaa ggcccagttg catgggataa
    11521 gtatcttgaa gacaattatt ggtacacagc agagcctgca agtgattttt accataaata
    11581 tcctgttgat ttagaattag ctgaaaaata tggcgttaat ggtattcgta tttcaattgc
    11641 ctggtctcgt attttcccaa ctggctatgg cgaagtaaat gaaaaaggtg ttgaattcta
    11701 ccataatctt tttgccgagt gccacaaacg tcatgttgag ccattcgtga ctttgcacca
    11761 ctttgacaca ccagaagttc ttcactcaga tggagatttc ttaaaccgcg aaaatataga
    11821 acactttgta gattatgccg ctttctgttt tgaagagttc ccagaagtaa actactggac
    11881 aactttcaat gaaattggcc caattggaga tggtcaatac ttggttggta aattccctcc
    11941 aggcattcaa tatgatcttg ccaaagtctt ccaatcacac cacaatatga tggtttctca
    12001 tgcacgtgct gtgaaattgt ataaagataa aggttataaa ggcgaaattg gtgtagtaca
    12061 tgcgttgcca accaaatacc cttatgatcc agaaaatcca gcagatgttc gtgccgctga
    12121 gttagaagat atcatccata acaaatttat cttggatgca acctaccttg gacactattc
    12181 agataaaacg atggaagggg tcaaccacat cctagctgaa aatggtggag aacttgatct
    12241 tcgtgatgaa gacttccaag cacttgaagc agctaaagat ttgaacgatt tccttggtat
    12301 caactactac atgagtgatt ggatgcaagc ctttgatggt gagactgaaa tcattcacaa
    12361 tggtaagggt gaaaaaggaa gttctaagta ccagattaaa ggtgttggtc gccgaatcgc
    12421 accagattat gtgcctagaa cggattggga ctggattatt tatcctgaag gcttgtatga
    12481 ccaaatcatg cgagtgaaaa atgattatcc aaactacaag aagatttaca tcactgaaaa
    12541 cggtcttgga tacaaagatg agtttgtaga taataccgtt tatgatgatg gtcgtattga
    12601 ttacgtgaag aaacacttgg aagttttatc agacgcaatt gcggatggtg caaatgttaa
    12661 aggttacttc atctggtcac ttatggatgt cttctcatgg tcaaacggat acgaaaaacg
    12721 ttatggacta ttctatgtag actttgacac gcaagaacgc tatcctaaga aatcagctca
    12781 ttggtataag aaagtagcag aaactcaagt gatagagtaa tattacttga aaatgtaaaa
    12841 aattgttatc cgatacttta gaaaaagtac aacacttatt tagaccaggc tttgaaaagg
    12901 tatatattgt tagtttcgaa gattgtccta tgattcctga gcttgaagct accccgttgc
    12961 taaaatgtgg taaatggtat gtttcaactg gaaaagagtg gatttgccat tctgatttag
    13021 agttatcagc atttgaatgg gaatttttac aatcactcga tgtagaaata cgtgagacta
    13081 ttcacttcga ggtaaattat ttgcctttcc aataataaaa agaaaggaat tagttgtgac
    13141 tatcgaatta aaaaacgagt atcttactgt tcagtttaaa acacttggtg gtcaactgac
    13201 ttcgattaaa gacaaggatg ggatagagta tctctggcaa gctgatccaa attattggaa
    13261 tggacaggcg cctattctat ttcctatctg tggtagttta cgaaatgatt gggctattta
    13321 tagacctcaa gaaagaccct tttttacagg tcttattcgt agacatggtt ttgttcgaaa
    13381 agaagaattt attctggaag aagtcaatga taatagcgtg acctttagta ttaaaccaaa
    13441 tgctgagatg cttgataatt acctatatca gtttgaactg agagttgttt ataccttgaa
    13501 tggtaaatca attagaaccg aatttcaggt aacgaacttg gaaatggaaa aaacaatgcc
    13561 ttactttatt ggagcccatc cagcattcaa tagtccttta gtagaaggtg aaaactatga
    13621 ggattattat attgaattta gtgaggttga gtcttgctcg ataccaaaga gttttcctga
    13681 gacaggatta ttagatttgc aagatcggac accttttttg gaaaatcaaa aaattctcga
    13741 tttggactac tcactttttt cccatgatgc cattacactt gaccagctca agtctcgtag
    13801 cgtaacgctt cggtcaagga agtctggtaa aggtcttcga gtagattttg atgattttcc
    13861 gaacttgatt ctgtggtcta caactaataa aagtccattc attgctttgg aaccttggag
    13921 tggtctctcc acttcacttg aagaaggtaa tttcttagaa gataaaagac aggtaacgaa
    13981 aatttcacct caagaaactt ctagaaaaat ctatgatatt actatcctta attaaaattt
    14041 gttattattt gagtcttcaa taagatttga atactcttac cttgtgatga tagattggtc
    14101 ttctccgtgc cagtcttaca tcgcaaggtt tttaagtttt ttgtgggtct atgtcaactg
    14161 tagtgggtga cgaaaagcta atatctaaag acagtctggt agtaaaaaat aagattattt
    14221 actacttctt gatttaaaat aatagtggag ccattaattt gtggcaaatt taatttaata
    14281 ttttgcctca aattagccac actttttata gaactaagag gaaaaatgaa attttcttcc
    14341 tattaatata gttgtttgaa atcttaaata tagcgt
//
LOCUS       DAGCXG010000060        14374 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGCXG010000060 DAGCXG010000000
VERSION     DAGCXG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.063"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1226)
                     /gene="rpsA"
                     /locus_tag="MB022_001811"
     CDS             complement(<1..1226)
                     /gene="rpsA"
                     /locus_tag="MB022_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBT4466806.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSE"
     gene            complement(1361..2041)
                     /locus_tag="MB022_001812"
     CDS             complement(1361..2041)
                     /locus_tag="MB022_001812"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBT4466807.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2090..2725)
                     /locus_tag="MB022_001813"
     CDS             complement(2090..2725)
                     /locus_tag="MB022_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4466808.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2791..4194)
                     /locus_tag="MB022_001814"
     CDS             complement(2791..4194)
                     /locus_tag="MB022_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBT4466809.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(4181..5212)
                     /locus_tag="MB022_001815"
     CDS             complement(4181..5212)
                     /locus_tag="MB022_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466810.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5243..5464
                     /locus_tag="MB022_001816"
     CDS             5243..5464
                     /locus_tag="MB022_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBT4466811.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(5629..6366)
                     /locus_tag="MB022_001817"
     CDS             complement(5629..6366)
                     /locus_tag="MB022_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBT4466812.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(6519..7859)
                     /locus_tag="MB022_001818"
     CDS             complement(6519..7859)
                     /locus_tag="MB022_001818"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBT4466813.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(8096..9619)
                     /locus_tag="MB022_001819"
     CDS             complement(8096..9619)
                     /locus_tag="MB022_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBT4466814.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(9997..10608)
                     /locus_tag="MB022_001820"
     CDS             complement(9997..10608)
                     /locus_tag="MB022_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4466815.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(10731..10856)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(10938..11654)
                     /locus_tag="MB022_001821"
     CDS             complement(10938..11654)
                     /locus_tag="MB022_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4466816.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(11660..12229)
                     /gene="scpB"
                     /locus_tag="MB022_001822"
     CDS             complement(11660..12229)
                     /gene="scpB"
                     /locus_tag="MB022_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBT4466817.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(12213..13010)
                     /locus_tag="MB022_001823"
     CDS             complement(12213..13010)
                     /locus_tag="MB022_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBT4466818.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(12980..13351)
                     /locus_tag="MB022_001824"
     CDS             complement(12980..13351)
                     /locus_tag="MB022_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4466819.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(13370..14257)
                     /gene="xerD"
                     /locus_tag="MB022_001825"
     CDS             complement(13370..14257)
                     /gene="xerD"
                     /locus_tag="MB022_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBT4466820.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4017 a         3109 c         2443 g         4805 t
ORIGIN      
        1 ttttcagaat catcggttgc tttttcttct acttcttctt tcaacgcatc acctaaaatg
       61 tctcccatag taaatccagt attttcttct ggtaattcat aagactcaac attttttggt
      121 tcttcttctg aagctggttt ttcttctaaa gctttgatac tcaatgcaat acgatgttct
      181 tctggatgga tctctaatac tttcactttc acttcgtcac cttcatgaag cacttcatgt
      241 ggtgtcgcaa tatgtttatg tgaaatttga gagatatgga ctagcccttc tactccaggg
      301 aacacttcaa caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg
      361 ccaactggag cttttgtatc gatttctgac caaggtccag gctgtgtgtc tttgattgac
      421 aaggaaacac gttcttgttc aggattgata gacaacactt tgacatttac atcatcattg
      481 acagccaata catcacttgg ttttgctaca tgactatgtg aaatctctga gacatggact
      541 agaccgtcaa ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt
      601 ccactgagaa catctccctc atggatagtt gaaagaattt cttttttctt ttcttctctt
      661 tcgttttcca ctaccgcttt atgcgataag atcaaacgat tttctgatgg ttcgatttcg
      721 atgattttaa agctcaatgt tttgccttta tagtctgaaa agtctgcaac aaaatgatct
      781 tccaccattg atgcagggac aaaaccacgt acaccgacgt caacgactaa accgcctttg
      841 acaacatttg taacaggtgc ttcgatgacc ttgccctctt ggaaatctct ttcgatatct
      901 tcccaaactt ttttagcatc taaacggcgt tttgaaagta ggtagctgcc gttttcttta
      961 tctttgccga tcgtactgat aacgactaaa tctaatacat caccgacttt tactacttcg
     1021 ctgatatctt ctaccggcaa agttgataat tcttttgccg gaacgactcc ttcaactcca
     1081 gccccttcga ttccgacaat cacttgtttg tcttcaatga ctaaaacttc gcccttaaca
     1141 acatcgccaa cttttacttc ttgtacgctt ttcattgcgt cttccattga ctggttgttc
     1201 tcttgatttt gatcaaattc tgtcataaca cttgtcctcc taaccaacat tctgccgtaa
     1261 gtacggtact gtttttagtt taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt
     1321 ttccttgaaa aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga agataacctt
     1381 ttttcaaaac aacagctttt attgctgcaa cgacttctgg aatcgatttg ccagtcgtat
     1441 cgatacgaac agcatcctct gcttgcttca gcggagagac gtcccttgtt gaatcataat
     1501 ggtctctttc ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt cctttttctt
     1561 ggttctcttt gaaacgtctt tctgcacgct cacttacgct tgctactaaa aagattttca
     1621 cttctgcatt cggtaataca gctgtcccga tgtctcttcc gtccatgaca acgccaccgc
     1681 gttcccctat ttttcgttgg acagcaacta attcttctct gacttttcca tgtgcagaga
     1741 cttcagaaac agcttttgtg acatctggtt gacggatttc taaagtcaca tcttttgatg
     1801 ctatgaatac atgctgtcca tcttcttgtt gcgaaaaaga gataggatac tttttgatca
     1861 gcgaaactag cccttcttca tctgaaaaag atacattgtt cgtaatagct aaataagtta
     1921 ctgcgcgata catggcaccg gtatctgtat aaatataatt caattctttt gctaatatct
     1981 tggcaacggt acttttccca gatgaagcag gcccgtcgat tgcaatactg atttgtgtca
     2041 tgatataact cctttttctc agaaaaaaag ccgcaaagcg cggctattat tatttaattc
     2101 ttaattcttg accagggtaa agcataaagt tgtttggatc taagccattc aattcaaaca
     2161 attgatcagt ggtgatccct gcacgctcag ccacttgttt tggaccttcg cctgcttgaa
     2221 cagtcgttgt tgaaccggct gcagctgatg aagaaggtgt tgtttctgct gcattgctgc
     2281 ttgaatcccc tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa
     2341 tactgctttc tgacgttaca cctgtatctt gagacacagt agattcttcc gcgattgtac
     2401 tagattctac tgttgaagtc tcagttgact cctcttttgt tgtacttgta gaactttcta
     2461 ctactgatgt cgaagtttca gaagaagttg ctgctgttgc attgttgctt gatccatttc
     2521 tgatccagaa ataagcacct gctggaatag cgatacataa gaccagtaaa atcaaaaata
     2581 gcgaaagaaa taatgtattt cctttctttt gttgtctctg agaacttctt gatgaaactt
     2641 catcatctgt atcgtagatt ggttgttccc atggttcttg tgtttcgtta ttttctgtag
     2701 attgatgtct atcttttctg ctcactgttt tacctcctaa actctatcgt gtgtattata
     2761 ccataggaaa aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt taaataaatt
     2821 tagtaaaatc gtttcccaat gaagttgttc agtcttagaa aaaagcttcc tctttggcag
     2881 aagttctgct tttgctccat caatatcaca acaattctgc ggcttttcgg agagtttttc
     2941 gccaaaataa gctaagatga attttctgcg gcagttttct tcattaatat agcgaagcat
     3001 ctgctgcagg cggaattctt tttctttttc ctgacgtttg agccgttcca cccaattttc
     3061 tggtttttca gactgctgga tcagctccat ccattttttt tgcagctcat caaagggagc
     3121 ttgctttgtg gcatattcca aataaagctc aaaactttgt cgctgttctc tagaaagttg
     3181 attaaagaaa taatggatac gttcatcccc actttgataa aaaagaattg ctgcactttc
     3241 ttcctgatct ctgcctgctc gaccgatttc ttgtacatag ttttctagac tgtctggcaa
     3301 atcataatgg accacataac ggatatcaga tttatcgatc cccataccaa atgcattcgt
     3361 agcaatgaga aactgcagct gattttttac aaactgctgt tgaagttgtc ttctttgagc
     3421 tgcatcaaga ccaccgtgat agtatccaac tgtaaaacgt tccctgaata aatgatacaa
     3481 tcgttcgact tctttttttg ttgcacaata aatgattgcc gctccttgtg ctctttccat
     3541 gaagtgttca agttcttgtt ctttttgctg cgttttccga acaaaaagcg agatattttg
     3601 ccgattgact gattgtctga tctcttttgg tggtcgatca aatagtacat gacgtatatc
     3661 tcttgctact aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag
     3721 ctttcggatt tttcctaact gttgatattc tggacggaaa tcaactcccc attgagacac
     3781 gcaatgagcc tcatctacta catataacgc aatattttgt tttttcagat gctctaaaag
     3841 agaaggatta gtcaacattt cgggactcaa aaacaaaaat ttatattgcg atagatgacg
     3901 gaggacgtat cttctttcag attgcgaaag taaactattg aaagcagcta ctcgtttctc
     3961 ccctcgtttt tgtaattgcg tcacttgatc ttccattaga gaaagcagcg gtgacacgat
     4021 gatgaccaga ccttccatta aataccccgt caattgatag caaaggcttt ttcccgttcc
     4081 tgtaggcaaa atagcgagag tatcttctcc atcaagcaac gcctgaataa tttcttcttg
     4141 gccgggacga aatgaatcaa atccaaaata tttttttaat tcatttatta acattctgta
     4201 tcgcttcctt tctcaataga tagatttgat acaaacgaaa ttctccataa tctaattgtc
     4261 catctacatt ccattcgcta aagcgccact ctcgcacgga atccttattt ggttccaaac
     4321 gttcgattgt ttctgcagag agaatccact cgtatggaaa atcatcaaaa aacaaggccc
     4381 actcaataag atgatctgtt actgttcctc tttttaaatg acggattgcc atgatctggt
     4441 caattgtctg accattcata aacatctgct tggtgatcat catgctttga ttgaaatttt
     4501 gctgtaataa tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg
     4561 ctaacaaaag gtggatacat ctgctttgtt gcagataaac ctctgactca tcattgtttt
     4621 ttgccagctg ataaggaagc agccctgttc gctcatttcc agataattgg ttagctaaaa
     4681 aatctgctgc ttcttctggt atctccgaaa acagctgcac caaactctca tacgcagtat
     4741 caatcaaaat ttcacgtggc aattggcttt cactaagcca tttttttagt tggaaagtat
     4801 aaaaaggact tgtctctgct ggaagatagt cttgaatacc cgttgccaga ttagaaatta
     4861 cttgaacagc aaatttaatg agccgccaac tagtttcccc tgttctgcca tagcggtcaa
     4921 aacgcaaacc agtatgttct atttgatgtt cggacaacat atcagcccct gcaggcgtta
     4981 gctttgcttc attttcattt atttcgatcc aaccttgctg cgcaattttt tgcaaaattt
     5041 gataaaactt atcctgcttc aattctggaa aactgttatg gataaacaaa agttcgtggg
     5101 taaatccata aataagtact gaagtcgttc gttttcccac aaggagttgg taaagagaac
     5161 taatccgcag cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc atattattct
     5221 ccttaagtta ggatggttag aaatgtcact actatgtaaa cttgtacctg aacgctgtat
     5281 tgcatgcggt ctttgtcaaa tcgaagcgcc agatattttc gattatgatg aagaaggaat
     5341 cgttttattt gcccaagaac cagaggctca tcaacagttc gtgatgccgg aagaagaaac
     5401 atatgtaaaa aaagcgtatc aaaaatgtcc agttcgagcg attttattag aaaataaaat
     5461 ataaatataa acgaaacttt aaaaacgtac actaagttct ctaaacttga aaataagccg
     5521 ggatctgttt ctcttgttcc gtgaaatccc gacttatttt gaaagattca tttgctatct
     5581 atagttaatt gactagtttt ttcacggacc ctataggacc catttatatt atttcaactt
     5641 tttcagccag ttttccaatg tattgacgat cacagtttgc tggtctgcgt tagaaatttc
     5701 tgcctctcca tctccttttt gctcaccata gctgccaaaa cctccatgat ttccgccatt
     5761 gattgataca tactctgttc tttccggtaa atactgctta gcttcttgat aagcttcctg
     5821 attcaatact ttatcttttg tcgccgtgat cgataaaaca ggaacagctg attctgtcaa
     5881 tgttcctttt ttgtcgggat aacttgccat gaagaatacc ccttttaaat gtttgtctga
     5941 atgattctta ttgacataac gacttgccat tacgccacct aatgaatggc caccgatcac
     6001 ataatcggaa tttggaaaat cttctttgac cttatccgct ttgtttccgc ccatcactgc
     6061 tagatcaagt ggcattttga caatagcgac tgaaaagccg ttttttgcta aatcttgcgc
     6121 ccaaatacta taactttctg gctctacgag agcaccagga taaaaaataa gtaatggaga
     6181 ctttccatga ttctttttct ctccttcaaa aaataagtaa tcttctttgt tttttccttc
     6241 ttttaccaac acttgcgctt ctgacgatgg ttcgtacgta ttttgctgta tatagtaata
     6301 accggctcca ccgagtaatc ctaaaatgat gactaagcca attagtattc gtctaaatat
     6361 tttcactttc tcccaccttt tctatctgag tgaccccttt ttcttaatca agtatgatta
     6421 taccttaaaa aaataagaga gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa
     6481 tatccatttg aatatccgcc ctttttgtat gtttattttt atttagcaca taaacgatac
     6541 acagcttctg cataaatttc cattgcttta tacatactac ttaattccca tcgttcattc
     6601 gcctggtgca taaagtcagg agtatcaggg aacatcgcac cgaatgccac acactggttc
     6661 atcgtacgag caaaagtagc gccgcctgat acaaacggtt cagtcatgtc tccagtaata
     6721 tcgcgatatg tccccaatag atttttcact aactcactgt ctagtggcac gtataatgaa
     6781 tcaaggaagt caaattcttc ataagtcaac ccatattttt ctgctgtttt agttaattta
     6841 gctactaaat catctttctt gaatgtaaca gggattctca tatctacacc gatttttgtt
     6901 tgttctggtg tgatttccaa acttgcaaag ttcatcgtta gctcgccgga ttgttcatct
     6961 tctgttttgc ccacgacagc ttcaccagtt gcattttctt gaactaattt tcctaagaaa
     7021 ttgattgggc caaaatcaaa tacttctgaa agagcaattg ccagacgaga aatagcattc
     7081 actccttgtg ctgcatcttt cgcgtggata cttttaccaa gaacgacgat gccttcacct
     7141 tgttcttcga aatcgaattc gtgttttttc aatgcttctt ttacttcaga aagcttttct
     7201 ccagagtaag gagctgcatc aggaacaaca ttcaacgctc ctccagcttt tacagaaaac
     7261 tcgttcgttc ctggtcctgt caagtaagct tgcaataagc ctttttctgc ataaattaaa
     7321 ggaaattcag catctggcgc aaatccttgc gtaattcctt cttctttttc gttatatttt
     7381 tctaaacaac gccataaatt ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg
     7441 aattccacgc cagcatcaat caaagctttg acggcataca ttgccgcaat agaaggacct
     7501 ttgtcatctt gggatccgcg accaactaac cagccgtctt ttatagttgg atcgaatggt
     7561 tttgtttccc aattattttc gtcccctgct ggaacaacgt ccatatgaca caagataccg
     7621 aataattcat ctccagagcc aatctctgaa taaccgtaat agccttctgg atctttaaat
     7681 gttctaaatc ctagcttttc actgatttcc aatactttat ccaaagcttc aatcactttt
     7741 ttaccgaagg gatgtccttg tcctgaatct gcttcatcta gtacggaagg aattcggatc
     7801 aattcgctca atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg
     7861 atattatcct cctaatatgt taaaaagggt catgtcaaat tcctccgaca caacctcttc
     7921 ttctcaaata atgacgtttt aagcctgctc catcagcaag cagaatgttc ttaacaaatg
     7981 atgaaagaaa aaacactcac tgaatggagc tattgacttg tccacgtgct tatcgatagc
     8041 cgtttatcgg tttcttcatt cttatgatag accttgtacc tgtatttgtc taggtttaga
     8101 acattgctgc tacacctagg atcacacaga tggctacaaa taatatcgca attaatttac
     8161 cggtaaattt ccaccacact gaaacatcga aacgagcaat tgctacagca cccattacga
     8221 tagcagatgt tggtgtaatt aagtttacta aaccagatgc agattgataa gcagtgatta
     8281 ctaaatcctt accgacgccg gcaaattcac ccataggtcc catgattccc attgttgcag
     8341 cagctaagcc ggaagttgaa ggaatcaaga atgacatagg gatatagaaa ataaatgtaa
     8401 taatgatgaa tacacttgaa gataatgaac ttaatccttg ttcgccccaa tgtaagatgg
     8461 tgtctgtgat taatccattg ttcataacca cttggatacc acgagcaaca gctactacga
     8521 ttgctacacc gatcaaatca gaagcaccag ctaaaaattc agagacaaat tcagactctt
     8581 tcatgccata agtgattgct actacgatgg acattactaa gaataacata gtaatttctg
     8641 ggaagtacca gtcacctaat ggaagcatgc ttttacctag aaccgtacct agtaccggaa
     8701 ggtttgttaa ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc
     8761 aaggaatcaa actgatgatc atgataccaa aagtaataaa gaacaataca ttaacatgtt
     8821 tttgtttttt cgtgattact tcttgacgac cgattgcttc aatatcgaaa tgcttcatat
     8881 cttctttacg ttggttgtat actaatgatt ttgttggatc tttttcgatc tttgatgcat
     8941 aacgatacac atatacaata gaaatagcta acaaaataac gaacatcaag aatcgaagac
     9001 caataccttc acccatcgaa atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat
     9061 ttaccgttga agctagacag cctacttgag accctactaa tacgattgct acagctacta
     9121 ctgtatcaaa gcctacagcc atcatgactg ggatcagtaa agcatagaat ggaatcgttt
     9181 cttctgccat tccgtaagta gagccgccta atgcaaataa cagcatcaat actggaataa
     9241 gcattttttc ttttcctttg aatttcttca caactgaagc aatcccggca tctagtgccc
     9301 ctgttttgtt gataatacct aaaaatccac caacaaccaa aatgaagaaa gagattggga
     9361 ttgcagcagg tgtttcttta gttagctccc ccgctggtac acctagcatc ccattgattg
     9421 gcgccatgaa aatatcccac agcccttgag gattttgttg tactcgttca tatgatccag
     9481 cgacgatgtt gcctgcatcg tctacactat agtgcccagc tggtataaac caagtaagta
     9541 cagcgattac tgcaataata ataaataaga ccgtatacgc tgaaggcatt tgaaattttt
     9601 tcttttttgt ttgttccatc ttcttcttcc tttcttttcc gttagagaca acaattttgg
     9661 ctctaatgtt attttatcaa aaagagtatc agaacaaaaa taataacacg cattttgtaa
     9721 aacttttgtt caaaaagttc gtttttccct attccctcct cttttttcag gtttttaaag
     9781 ctttcttctt tttcattgta gcgtttcctt ttttttataa acaaacattt tcgtctttta
     9841 aaaggagaag aatttcacat ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa
     9901 atactcaaaa caaacaaata aaacgtttac attattgcat gtcttgtatt tttttccaaa
     9961 aaaaaagacc aagaaatttc ttgatctttt tgatatttat ttgactgctg tatgacgttg
    10021 ttcgaactga tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa
    10081 gacagcactc acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata
    10141 gtttgctaaa gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag
    10201 aggcgtaata acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt
    10261 aatgacacct aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata
    10321 aatcgggaat gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc
    10381 cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc
    10441 ggccgctgga ccgaataaga acatgctgat caataccgag atatcactaa aatcgatctt
    10501 caaaaaacta aaagccggca tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc
    10561 agcaaacatc gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc
    10621 tgttaggact cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt
    10681 ttccagggaa aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc
    10741 tttatccaga ctatactgtc ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa
    10801 taggtcgtgg actataacca ccggtcggga atttcaccct gcccctgaag acgaacctta
    10861 tataattttt gtttacatat tcattataca tatctattca aaaaacgcaa catctaacac
    10921 ttataaaaag taagtgttca ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc
    10981 agttcacggt aatccccagg acgtaggcct tgtaaagtca gatctccgta tttttcacgt
    11041 tttagttttt gcacaggaag accaactgct tgaagcatat ttttcacttg atgattacgt
    11101 ccttcgtgga tcgtcaattc cacgatactg tggtttgtct taggatccac tgataaaatt
    11161 tggaatttag ctggagatgt ttttcgtcct tcgatcttta ttccttttgt caaaggtgca
    11221 agcatcgatt ttgtagcgat cccttttacc tttgcaacgt aaactttatc cacttcatgt
    11281 tttggatgtg tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa gatccccgaa
    11341 gtatcgtaat ccagtcgacc tacaggatag atacgttctt ttaccatcgg caaaagatcc
    11401 gtaacaactt ttcttccttt gtcatctgca acagcagaaa taactccttt aggtttgtac
    11461 aataaatagt aaccgtattc ttcttgataa attgggactt catccacttc cacagtgtca
    11521 tgtttcccaa cttttatccc taattccctt acaacttgtc cgtttacttt tacacgtcct
    11581 gcaaggatga attcttctgc ttttcgacga gaagtgatgc cagcgtgggc aattactttc
    11641 tgcaatctct ccattacaat tattctcctt tattcattcc atcaaagaat agatctggtg
    11701 acacgtcttc ttcaagtgaa atttccattt cttgtgtatc aggcagttct tctaatgatt
    11761 tcaaaccaaa ataatctaag aagtagccag ttgttccata taatatcggc cgacctggtc
    11821 cttctactct acctttttct tcaatcagtt tatgggcagc tagacgctgt accgctcctg
    11881 aaacctgaac tcctcgaatc tgctcgattt cagctcttgt gattggctgt ttataagcga
    11941 tgattgctaa ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc
    12001 gcttaagtaa gggagccagc tcttttttag tcgttaatac ataatggctt tccgtttcaa
    12061 aaatatgcaa agctgagtgc gagttttgag tgtaagattc ttttaattct ttcactaaac
    12121 ttgtgacttc tgcttcgctg agatttaaca gtccgcttaa ttcttcatta ctgatccctt
    12181 cctcgccaac aataaaaaga agggcttcaa gttcacttaa tttcgtcatg ttcggtatct
    12241 cctactgctg gataaagcat aattgattca tagttttctt tttgttgcgc aatgatctgc
    12301 ccttttttca tcaattctag caaagccata aaagtagtga caacttcact tttagaaggt
    12361 gactcaaaaa aagaatcgaa agtacagcca tgcgcatttc tttttatacg ctcttcgata
    12421 aataccattc gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc cactttttga
    12481 gaaaatttct tacgttctaa gattttctgc atggcacgga tcaaatcgat ttttttgact
    12541 tgtcctcgct tcaacggctt ttcagcatcc gctaacccat cgatgttcat aggttctttt
    12601 gtgtaataaa gacttcgttc ctctgctttt tctgtcagtc tttcagctgc atatttatat
    12661 ttacggtatt ctaaaagctg agtgaccaat tcttctcgag gatcttcttc ccataattct
    12721 tcatcgtctg tgccgaattc ttggacagga agcaatgtct tgcttttgat tgccatcaat
    12781 gtagcagcca tcactaagta ttctcctgca acagctagct cgagtgtttt cattgtatgg
    12841 atataattca tatattgctc cgtgatcgta gcgatcggta tatcatatat atcgatttcc
    12901 atttgctgaa tcagatgaag cagcagatcg agaggaccct cgaatacatc taatttaata
    12961 ttgatctccg tcaactcatt cactctttct tctttttctt tgctagccat tttccaatgt
    13021 aaggctctgt gatttcagtt gcacggatgg agacatccgg gtacttttcc tgtaattcat
    13081 ctaacatttg aaagcccata ttctcaccgc gataagacgg attcaaagaa atgtcatgga
    13141 tgatcagctg atcgttcgtt gcccagtaaa taccaataat cccttgaatg ttggttgaac
    13201 cttctggcgt aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta tcaatctcct
    13261 tcaacaaaga agttttacct gtcagtttct tatggaaact caatagtccc atagcaattt
    13321 tacggttttc tttccggtaa tgtgttaaca tcttctcacc ttcctatgac taagcccttg
    13381 gaaaatgctg cttataaaca tccgtcattc ttttcttcgt gatatgtgta taaatctgag
    13441 tcgtagagat atccgcgtga cctaacaatt cctgtactgt ccgcaaatca gctccatttt
    13501 ctagcagatg tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc
    13561 cagcctctcg tactaactgc ttcagatttt tccagattcc ttgccgagaa agacctttcc
    13621 catgattatt gacaaataca tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt
    13681 cgtctaagta gcgttccagc cattggatcg cataatcacc taaaggaatg atccgctctt
    13741 tatctccttt tccaactgtc tgtaccagtc caagagaaag atgaagatct cccaatttca
    13801 atccaatcaa ttcactgacg cgcatcccag tagcatacat gacttccaag attgcacgat
    13861 ctcttatccc taacgttttt gtcgtatcag gtgcttcaat cagcttttct acttctgtca
    13921 gagtcaatgt acttggtaat ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat
    13981 catgatcagt gatacgctct tgccgtaaaa actgatggaa tcttctgaga cttgagatca
    14041 tccgagtaac agttgctgtc gctttttttt cttcatgcaa cttctccaga aaacggatga
    14101 ctaaaaagcg atcgacatcc tgccatgaag caattttctg ctcctgtaga aaatgcaagt
    14161 actgttccaa atcacgttca tagctttgtc gtgtattggc ggacaatccc cgttcaatat
    14221 tcaagtagtg gagataatcg ttgatttgct ctttcataat aatccccctc atgcctctct
    14281 atcatatcag ataccgcttc attctgatag tctttttcca taatctgagc aaagaaaaag
    14341 ccgtctagac actcagacgg actggtttct tttt
//
LOCUS       DAGCXG010000061        14262 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGCXG010000061 DAGCXG010000000
VERSION     DAGCXG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.064"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(66..428)
                     /locus_tag="MB022_001826"
     CDS             complement(66..428)
                     /locus_tag="MB022_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466821.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(502..882)
                     /locus_tag="MB022_001827"
     CDS             complement(502..882)
                     /locus_tag="MB022_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBT4466822.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            946..2139
                     /locus_tag="MB022_001828"
     CDS             946..2139
                     /locus_tag="MB022_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4466823.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2216..2800)
                     /locus_tag="MB022_001829"
     CDS             complement(2216..2800)
                     /locus_tag="MB022_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4466824.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3001..3999
                     /locus_tag="MB022_001830"
     CDS             3001..3999
                     /locus_tag="MB022_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4466825.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4111..4620
                     /locus_tag="MB022_001831"
     CDS             4111..4620
                     /locus_tag="MB022_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBT4466826.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4650..5309)
                     /locus_tag="MB022_001832"
     CDS             complement(4650..5309)
                     /locus_tag="MB022_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBT4466827.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5311..6075)
                     /locus_tag="MB022_001833"
     CDS             complement(5311..6075)
                     /locus_tag="MB022_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBT4466828.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6083..6760)
                     /locus_tag="MB022_001834"
     CDS             complement(6083..6760)
                     /locus_tag="MB022_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBT4466829.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6797..8182)
                     /locus_tag="MB022_001835"
     CDS             complement(6797..8182)
                     /locus_tag="MB022_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4466830.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8392..8476)
                     /gene="ffs"
                     /locus_tag="MB022_001836"
     ncRNA           complement(8392..8476)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MB022_001836"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8499..9014)
                     /gene="tadA"
                     /locus_tag="MB022_001837"
     CDS             complement(8499..9014)
                     /gene="tadA"
                     /locus_tag="MB022_001837"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBT4466831.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9121..9978
                     /locus_tag="MB022_001838"
     CDS             9121..9978
                     /locus_tag="MB022_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466832.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10059..10394)
                     /locus_tag="MB022_001839"
     CDS             complement(10059..10394)
                     /locus_tag="MB022_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466833.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10461..11831)
                     /locus_tag="MB022_001840"
     CDS             complement(10461..11831)
                     /locus_tag="MB022_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBT4466834.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEITLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11909..12445)
                     /locus_tag="MB022_001841"
     CDS             complement(11909..12445)
                     /locus_tag="MB022_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4466835.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12754..14235
                     /locus_tag="MB022_001842"
     CDS             12754..14235
                     /locus_tag="MB022_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4466836.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4386 a         2853 c         2331 g         4692 t
ORIGIN      
        1 ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct
       61 gtattttaga agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga
      121 cctggttctt cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt
      181 tcaaagacaa acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa
      241 ctaatcttgc taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa
      301 ccagtctttc cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg
      361 accaaaaatt ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg
      421 cttgccaaaa tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca
      481 ttttcagaaa aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc
      541 ttcttctatc caaataggta actgctccgc aatagtggta aatccgatct tacgattttt
      601 attcgtgaaa tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct
      661 taaagataaa ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg
      721 ctgtccgact tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac
      781 atgaataagt ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc
      841 tgtcactttt ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc
      901 atttatcatt atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt
      961 aagatgatcg aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac
     1021 gcaattgctg agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac
     1081 ttcttgtctc ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt
     1141 ggttatgcca ctctgtctga taaactatat caggctatca ttcattggga acgccaacac
     1201 catgctgtgg agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta
     1261 gctgcagcag tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt
     1321 tatcctccat tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg
     1381 ctagaaaaaa ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa
     1441 aacaatgtaa aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc
     1501 aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt
     1561 gacgagatcc atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt
     1621 gatcctaatt tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta
     1681 gctgcaatca aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa
     1741 aaacatttag tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa
     1801 gcggcttatg agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat
     1861 gttcaaacag ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct
     1921 gaaggaactt acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg
     1981 gagaacactc ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct
     2041 tccggtcatg ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga
     2101 cttaatcgaa tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag
     2161 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2221 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2281 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2341 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2401 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2461 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     2521 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     2581 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     2641 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     2701 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     2761 gtcctttttc actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt
     2821 gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
     2881 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
     2941 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
     3001 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
     3061 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
     3121 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
     3181 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
     3241 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
     3301 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
     3361 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
     3421 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
     3481 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
     3541 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
     3601 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
     3661 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
     3721 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
     3781 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     3841 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     3901 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     3961 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     4021 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     4081 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     4141 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     4201 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     4261 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     4321 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     4381 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     4441 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     4501 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     4561 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     4621 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc
     4681 aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat
     4741 aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg
     4801 ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga
     4861 tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac
     4921 ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc
     4981 gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa
     5041 aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag
     5101 ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa
     5161 gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg
     5221 atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat
     5281 tccatccact gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt
     5341 cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa
     5401 aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc
     5461 tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat
     5521 gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga
     5581 gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt
     5641 cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata
     5701 ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa
     5761 tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc
     5821 ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg
     5881 agctacatga atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt
     5941 cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg
     6001 tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata
     6061 tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg
     6121 ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact
     6181 ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct
     6241 catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt
     6301 ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa
     6361 gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga
     6421 aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg
     6481 ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg
     6541 acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta
     6601 gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca
     6661 gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt
     6721 tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt
     6781 ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac
     6841 ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa
     6901 atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc agatcgattg
     6961 gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca
     7021 tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt
     7081 tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat
     7141 ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt
     7201 ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt
     7261 ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca
     7321 gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat
     7381 aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt
     7441 tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat
     7501 actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga
     7561 ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt
     7621 ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta
     7681 aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat
     7741 tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg
     7801 ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata
     7861 agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg
     7921 aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca
     7981 gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat
     8041 cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt
     8101 gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat
     8161 gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag
     8221 gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa
     8281 ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt
     8341 acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca
     8401 gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc
     8461 caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag
     8521 tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc
     8581 ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc
     8641 tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa
     8701 acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta
     8761 acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt
     8821 gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt
     8881 ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct
     8941 gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga
     9001 tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg
     9061 ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag
     9121 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     9181 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     9241 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     9301 aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     9361 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     9421 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     9481 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat caacggtatt
     9541 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
     9601 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
     9661 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
     9721 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
     9781 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
     9841 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
     9901 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
     9961 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
    10021 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
    10081 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
    10141 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
    10201 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
    10261 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
    10321 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
    10381 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
    10441 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
    10501 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
    10561 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
    10621 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
    10681 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
    10741 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
    10801 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
    10861 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
    10921 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
    10981 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
    11041 tgagtcaaag taatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
    11101 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
    11161 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
    11221 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
    11281 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
    11341 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
    11401 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
    11461 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
    11521 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
    11581 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
    11641 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
    11701 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
    11761 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
    11821 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
    11881 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
    11941 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
    12001 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
    12061 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
    12121 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
    12181 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
    12241 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
    12301 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
    12361 ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac
    12421 tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata
    12481 aataacataa ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc
    12541 cattaaaaat caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa
    12601 ataaacaata gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg
    12661 cgacactctg tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac
    12721 tatatacaaa aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag
    12781 ttacttgttc tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat
    12841 gacttacata ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc
    12901 aagcaatttt tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc
    12961 aaagaaaccg atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta
    13021 aaagaatcca atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta
    13081 ccaaaaaaat atttacttga acaactttat atatcgccct cctatctcga aaaactgaca
    13141 aaacagctca atcattcctt aaaaaaattc catttacgga tcctatctgt aaaaaattgc
    13201 tgtactcttg aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat
    13261 acatttggag gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac
    13321 agtacagatg agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta
    13381 actttattca tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc
    13441 actgaattga tggacttatt gcagcataca catgattatt cagaatactt tacttctttt
    13501 tcagtgagtc gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc
    13561 tattgttttt ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt
    13621 tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa
    13681 cagtttcctc tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg
    13741 gcagttgttt ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt
    13801 ccagaagcca cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa
    13861 atcgatacaa ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa
    13921 ttgatccaac acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata
    13981 accaaacagt ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa
    14041 gatgctgtta aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat
    14101 gagaaaattt cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta
    14161 tttggcaagt taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa
    14221 acaaagagga tgtgacaaaa ttcttgtcac atcctcttat tc
//
LOCUS       DAGCXG010000062        14199 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGCXG010000062 DAGCXG010000000
VERSION     DAGCXG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.065"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1835)
                     /locus_tag="MB022_001843"
     CDS             complement(<1..1835)
                     /locus_tag="MB022_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4466837.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="MB022_001844"
     CDS             complement(1832..2536)
                     /locus_tag="MB022_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4466838.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="MB022_001845"
     CDS             complement(2548..4857)
                     /locus_tag="MB022_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4466839.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="MB022_001846"
     CDS             complement(5052..5516)
                     /locus_tag="MB022_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466840.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="MB022_001847"
     CDS             complement(5516..6142)
                     /locus_tag="MB022_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4466841.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="MB022_001848"
     CDS             complement(6149..6514)
                     /locus_tag="MB022_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBT4466842.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="MB022_001849"
     CDS             complement(6504..6842)
                     /locus_tag="MB022_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466843.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="MB022_001850"
     CDS             complement(6832..7158)
                     /locus_tag="MB022_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4466844.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="MB022_001851"
     CDS             complement(7136..7417)
                     /locus_tag="MB022_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBT4466845.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="MB022_001852"
     CDS             complement(7419..8783)
                     /locus_tag="MB022_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4466846.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="MB022_001853"
     CDS             complement(8796..9365)
                     /locus_tag="MB022_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBT4466847.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="MB022_001854"
     CDS             complement(9337..10524)
                     /locus_tag="MB022_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4466848.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="MB022_001855"
     CDS             complement(10588..12315)
                     /locus_tag="MB022_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4466849.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="MB022_001856"
     CDS             complement(12312..12764)
                     /locus_tag="MB022_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBT4466850.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="MB022_001857"
     CDS             complement(12876..13256)
                     /locus_tag="MB022_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4466851.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="MB022_001858"
     CDS             complement(13253..13639)
                     /locus_tag="MB022_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466852.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="MB022_001859"
     CDS             complement(13641..13919)
                     /locus_tag="MB022_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466853.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
BASE COUNT         3928 a         3057 c         2234 g         4980 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatac
//
LOCUS       DAGCXG010000063        14019 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGCXG010000063 DAGCXG010000000
VERSION     DAGCXG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.066"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..178)
                     /locus_tag="MB022_001860"
     CDS             complement(<1..178)
                     /locus_tag="MB022_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4466854.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLAS"
     gene            complement(657..1376)
                     /locus_tag="MB022_001861"
     CDS             complement(657..1376)
                     /locus_tag="MB022_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4466855.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1425..3071)
                     /locus_tag="MB022_001862"
     CDS             complement(1425..3071)
                     /locus_tag="MB022_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4466856.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3250..3840
                     /locus_tag="MB022_001863"
     CDS             3250..3840
                     /locus_tag="MB022_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4466857.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3932..5437
                     /locus_tag="MB022_001864"
     CDS             3932..5437
                     /locus_tag="MB022_001864"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBT4466858.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5521..6873)
                     /gene="celB"
                     /locus_tag="MB022_001865"
     CDS             complement(5521..6873)
                     /gene="celB"
                     /locus_tag="MB022_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4466859.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6873..7391)
                     /locus_tag="MB022_001866"
     CDS             complement(6873..7391)
                     /locus_tag="MB022_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466860.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7407..7733)
                     /locus_tag="MB022_001867"
     CDS             complement(7407..7733)
                     /locus_tag="MB022_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBT4466861.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7746..9725)
                     /locus_tag="MB022_001868"
     CDS             complement(7746..9725)
                     /locus_tag="MB022_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4466862.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9746..10060)
                     /locus_tag="MB022_001869"
     CDS             complement(9746..10060)
                     /locus_tag="MB022_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBT4466863.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10228..10521
                     /locus_tag="MB022_001870"
     CDS             10228..10521
                     /locus_tag="MB022_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBT4466864.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10599..11711)
                     /locus_tag="MB022_001871"
     CDS             complement(10599..11711)
                     /locus_tag="MB022_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4466865.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11864..12088
                     /locus_tag="MB022_001872"
     CDS             11864..12088
                     /locus_tag="MB022_001872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466866.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            12098..12277
                     /locus_tag="MB022_001873"
     CDS             12098..12277
                     /locus_tag="MB022_001873"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466867.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12471..12641
                     /locus_tag="MB022_001874"
     CDS             12471..12641
                     /locus_tag="MB022_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4466868.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13065..>14019)
                     /locus_tag="MB022_001875"
     CDS             complement(13065..>14019)
                     /locus_tag="MB022_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HBT4466869.1"
                     /translation="GNPAGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFE
                     LQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQT
                     AVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAI
                     DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNP
                     TSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4358 a         2872 c         2405 g         4384 t
ORIGIN      
        1 agctagccaa gaacccatct gctgaaatat gaaaattttt gaatgctacg acattcgcca
       61 ttacaggtat gtaaaagaaa aaaatcatcc aaagcatacc cggtacagcc atcaggacaa
      121 gtgctttata acgccataga ttcataaaaa aatgattgaa cccctttcgt tttttcatct
      181 aaaaacccct ttcgattcat tcgcaacgta attatagcgc tttcaataat gagaataaat
      241 aaacaaagta tagggaaaat gaaacaaagt ataggagagg ttgtgaaatc agcgttttgg
      301 cctgagtatt agagaaaaaa acgggaaaat agttcctcat attttgccgt tttttgagct
      361 aataccgagg gtcagctgat tgaacaccgt ttatcagagg ttgtgaattc agcgttttgg
      421 cctgagtatt agggagatcg tgtccttact atctaactct gagtcacaag gaacaatggg
      481 gactgtaaca aaagtcttgt cacagtccct tattccgaac aaacggtgtt ccagaagtag
      541 cttctacgga aataagccga aatttcacaa aaaagccatc cgaaagtcag acggacaagt
      601 ctgcttttca gatggcgcta caactactct gcttattctt ttagctatcc attcaattat
      661 ttttctctca cttgttctag ctcttctttg gtcatgggtc gatattgccc tttattcaaa
      721 tctttaggca gccaaaattt tcccatacgg attcttttta aatataagac ttcttttcct
      781 actgctttga ccattcgctt aacttggtga aactttcctt catgcaaaat cagacggata
      841 attgaagttt ctgtctcttg atctacttga tcaatttgga gttttgccgg caatgtctgc
      901 tcatcgccat caataatcag tccttctgca aataaacgga tatcttcttc tgtcattata
      961 ccgctaacct cagctagata ttctttctcc acatgttttt taggtgatag taactgatgg
     1021 gcaaacaccc cgtcattcgt taaaatcaag aggccctctg tgtctttatc taatcggccg
     1081 actggaaaaa gatcttcccg ataatcctgg tcttccaata aatcgattac ggtttgatca
     1141 tgattatcct gagtagcaga aatgactcct gcaggtttat gcaaaatgta gtaaaaatct
     1201 ttttgatacg taatgatttc tcctaaataa gtaaccgcat cttcgaattc atttacttgc
     1261 gctttatcgc ttttcaccac ttgtccattg acttcgatcg ttcctttttt gatcaatcct
     1321 ttgacttctt tgcggctgcc aagtcccata tctgctaaaa acttatctaa acgcatgcgt
     1381 ttcccccatt tcaaaagagc atcaatctag ctcttctgat tcacttattt tatttttaat
     1441 ttatgtcgta aacgcgctat tcctggtcct agtagtcttt ctgccaagcg gattttcaaa
     1501 gctaaataac catacactaa agcccctata cctgctacta caagaatcaa caatagagac
     1561 tggaatttgc tatcttcgtt taaaaaagca ccaaaaatca tctttgtgat ccacgaagcc
     1621 aaaagcataa tcaaggtaag aataaagatc agcaatgtcc tgcgccagac aaaacttggt
     1681 ttaaagcggg ctacttcatg gattctcttt aagatcaagg tacaagacac agcaaatccg
     1741 atcattgtcg ccaataaagg gccgtatact tcaaataaac gaatggctgg gtattgaaga
     1801 aggagtttca ctagaaaccc aataaccaaa taattaattg ccgccttgtt ttcaaacatt
     1861 ccttgcagca tgttggaaac tagcatatat agtcctagga agagtcctac aaaacttgct
     1921 tggatcagta cattacttcc tagagaatcc ggtgtataaa ataatgtatt tagcggatag
     1981 gcaagtaaca taacaccaaa tgtcgccgga aacataaaaa atgaaaacaa ctgtaaattt
     2041 ccgcttgtca attttgccag acctactcga tctttcaacg taacagcttc tgtaatcaac
     2101 ggtaacccag tagacgcgat cgatgttgcc aatgcaataa ctaccatcgt caacttatct
     2161 ggattggcac taaaaatcga aaaaagatcc agtaactgtg cattggaata ctcggtggat
     2221 gcagacatga tcttcataaa agtgaactga tcgactaatt tgaagatcgt tacccctgaa
     2281 cccacaataa taaacggaat cgcttcttta acggtatcta acaacaattc cttcgttgca
     2341 atatttattt tgttttcgct gtagtgaata aacgccgatg tatacgcttg atgtttcttc
     2401 aaaaaataca aaaggacaag aatacttgct agcataccga taaatgccgc gaaagttgat
     2461 tgcgtgaccg ccgtcacata gtcaccatcc atgactttca tgatgatgaa tgtagccaat
     2521 agcatataaa aaatacgtgc cacctgttct acaatctgcg acagcgcata aggcatcatt
     2581 tcttgattgc cttgaaaata ccctcgtatc acactcatac aaggaaaaac caatacagcg
     2641 atactcaaag aacgcatcgt tggaatcagt tcttctccgc caccagaagc atgagctagc
     2701 caaggagagg cgatgtacat gaacaaagca aacaacaggc ctagcccgcc catcatctgt
     2761 aatgcacgga taaataatcg tcgtgaagtg ccatattcat tcaatgaatt atagcgtgca
     2821 gtttgttttg caatcgcagc aggtattcct gctgtagaga tcagtagaaa caatgcatat
     2881 atattgtatc ccatattgaa taatccattg gcagccttag cattttcgcc catccaagca
     2941 taccagggaa tgatatagac agcgcctagc aatcttgaaa tgatattact ggcggtcatc
     3001 caagcagagc ctcgtgccat tttttcttgc ggtgttaatt gatctaatgc tggttttctt
     3061 tgattatcca tcggtcagat tccaactttc tacaaaacat tactatcatt ttaattggta
     3121 aaacagaaac tggcaatcga ttttatataa cttttataag aacaattgtt tttttgaatt
     3181 cggtttcgtt tttttggtaa agtaaagaaa tatacagcct tttatggcta acatttatag
     3241 gagatatcaa tgacacaaga aataaaaaaa ctacccgtta tatttttagg tcttttcttt
     3301 atcacagttg gattgaattg gttcttgctc cctcacgata tagcttccgc cggagtgggc
     3361 gccatcggac atttagttga aacggccttt tctatccaac gctcagttac tgtttggagc
     3421 atcaatctta tgatgctgct tctagccgct gtcctattag gaaaatcagt attttttaag
     3481 actgtagcgg gaagtctgct ttttcctgtt gttttagaga ttgttccaaa agtggaaatg
     3541 atctcctcac atttcttttc actgatttca ggaagtcttt tgttcagtct tggggtttac
     3601 acattgtaca cagtcggggc ctcaaatggc ggtataacga taccgccgat tattttccac
     3661 aaattttttc ggcttccaat gccgcaagga cttttactta ccaatagtct tatcgtcttt
     3721 ttgaattatt atgtttttgg attgttggag acactattcg ttctcctatc tattacagtc
     3781 agttcattgt ttatgaaaat actcatccgc ttgtcccctg tgtcaagaaa atttgaataa
     3841 agctggattt tgtcatagcg gaaacaaacg atatattaaa aagagtgtgc tataataaac
     3901 caagaaattg aataaagcga gggatcaatc aatgagcttc tctttagaag acatccgtca
     3961 tttactatta aaagaaaatc tattaaaaga atttgtttca acaagaggct ggcatcttga
     4021 tgtaccagaa aaagctgaat ttcttcagtt aagttatgat tcaagaaaag ctgatgcttc
     4081 tactttgttt ttctgtaaag gaatgaattt caaagaagaa tatttggatt cagcaatcga
     4141 acagggtatt caatattatg tatcagaaca accatacgaa aattcagcag taggaatcat
     4201 cgtaacggat atccgtaaag caatggcgct tcttgctatg gctttttacg actatccgca
     4261 aaataaattg aaagtgatcg gattcactgg aacaaaaggt aaaacaactg ctgcgtactt
     4321 tacaaaagca atcttggatc acacaacaaa taaaaaaaca gcattgcttt ctactatgaa
     4381 tacaacgtta gatggcaaaa catatttcaa atcccatttg acaacgcctg agtcattaga
     4441 tttatatcgt atgatgtcag aagcagttga aaatggtatg actcatctag tgatggaagt
     4501 ttcttcacaa gcttacaaaa cacaacgcgt atacggcttg actttagatg tcggaatctt
     4561 cttgaatatc tcgcctgacc atatcagtcc gatcgaacat ccgacatttg atgactattt
     4621 ctattgcaag cgacaattga tcttgaattc aaaaacagtt gttttaaatc atgaaagtga
     4681 ctattttgat ttacttaggg aaacagccga tctattccag attccaacga tcacttatgg
     4741 tcgttcggaa gaaagcaatt atcaagtgat ccgttcagat aaaggtactc acggttttac
     4801 tttatcttct catgaagatc gcttagctat ctgcgatact gcttatgata ttttgctggc
     4861 tggaggattc aaccaagaaa atgcagccag tgcgatcatt gccgcagcac taacaggtgc
     4921 ctccagtcaa gatgcacaag acggattaaa agaagctcgt gtaccaggcc gaatggatca
     4981 attgatccaa tccaatggag cgcatgtcta tgtggactat gcacataatt atctaagctt
     5041 gaaaacattg ctggaatttg ccaaaaatga acatccagat ggccgtgtaa ttgttgtatt
     5101 aggcagccca ggaaacaaag caatctcccg tcgacatgat tttggaaaag tgctatcaga
     5161 aacagctgac atcgcctttt tgacggcaga tgacccagca tttgaagatc cgaaaaaaat
     5221 cgcggaagaa atcaatgaag caattactaa tccagatttg atcgttcatt atgaaatgga
     5281 tcgtcctgaa gcgattcgac gcgctttagc agaaagcgct ccgcaagatt cagtcgttat
     5341 tgcgggaaaa ggcgtggatc cctatcaaaa aatcaatggt gtagatgaac cttatgaagg
     5401 ggattatgca atcgtgaagc ggttgattga agaataatgg tatttatata ctagtggttt
     5461 cccatttcta agaaaaaaag ggactgtgat tttttgtcac agttcctttt ttcttagaaa
     5521 tcattttaac ttttcactag ctaattctgc tgccttttct ctttcttcat ttaataattt
     5581 attatcatag aatttgataa atgggaacca gataatgaat gcgatgattg cacaaatgac
     5641 tgctaacaca gcagctttcc aatcaccacc actaccaaca aatgctccga tcccgactgg
     5701 tgaaggccac ggcatctgtg caagcatcgg tttgacgatt tccagccgaa tggcaaaata
     5761 agcgagtgta gcagaagcca tcggagctaa gaaaaacgga accgctaaat agggattgta
     5821 gacgattggc atcccgaaaa tgattggttc attgatattg aataatcctg gtacaagtga
     5881 ggcttttcct aacactttca gctgatcaga tttcgcgagg aatgcaataa agatgatcaa
     5941 tcctaaagtc gcacctgatc caccaacggt tacataagaa ttgttgaatt ctcctgccaa
     6001 cggataattt gcgccttgtg cgttagctgc catattagct aatacgatcg gggtcaagaa
     6061 agaagtgatg atattcgctc catggatccc tacgatccac aatgcatgga tcaagaaata
     6121 aatcaccatg acaccaagcc atgtattcgt caactgcgta acaaacccaa atgggatagc
     6181 aatcattttg aagatatccg ttcctagtgc aactaataat ccattgatga tcatcaccac
     6241 aaacgcaatg acaaatgttg ggatcagagc agtgaaagaa cgagaaactc ctggcggaac
     6301 aacatctggc atttttatga cccaatttct cttgacacac atacaataca attcgaccgc
     6361 aataatcgac ataataatag ctgtaaagat tcctgaagtt cctaaacgtt cgacaaaaga
     6421 gcccatccta ataccattta ctacagtttc tgtctcattg atagaagcaa tcaagctgat
     6481 tttaccatca gtcatgatca attcagggat acacataaag aatgcaaaaa cagatagtaa
     6541 tgcccccgtg agcggattaa catttaattt ttgttcatct cgttcaattg acgttaattc
     6601 ataagcaaac acaatggcaa agtaaataga taaaatcccc atcgtcattg tattgacgat
     6661 catgtacaag tcactaactt tgaaaaaagt tgcattaaaa aatccttcta aaaatgggaa
     6721 cggtaatggc aaaatattta aaaccaaaaa catcgagcct acaatcgtga aaggaataga
     6781 agccatcccc gctgccatga cagcccgaac gattttaagt tgcgcaattt tccccatagg
     6841 tcccatcagg tatttctgca aaaaatcaaa cattagttat tctcctttcc taaatataac
     6901 gctttttcat attgtttaat acggatatac tgttggtctt ggttgagtga aattttcttt
     6961 aaacgatact gtataaatgc actggcaaaa ataccgctta caacaagaat gaaaacaaat
     7021 aactgtcctc cattgttatt tgccataaaa ggatacaaga atgtaaatcc tgaggtaaac
     7081 aacactggaa tcactaataa attaaagaca ctctggaaca aaaaatacca ctttgtccat
     7141 ggaacgttcg tttggtgttt tcgataaagc tttacttgtt ctcctactgc aggcaaaata
     7201 gctaaaatca gaatcatcgg taaactactt cctaaacttt tagctgatag aagcagaatc
     7261 atccattgca tgttcatgaa aaagaaaaag gcagtcaggt aacgtacgat tagataacga
     7321 ttgaaataca tgtttttcaa actaagttgc tttttttcta attcgatttt cctttttact
     7381 gtatcctgca caatgatcct tcctttctat ttattttttt gatacaaata attcatttcc
     7441 tgtgcaactt ccaataatgt catcgtcgtc atcagatgat cttgtgcatg gaccatgata
     7501 atttccattt ctatttttgt cccgctggca tattctttca gaagttgagt ttgtgactga
     7561 tgcgcatgaa gaatctcttc atttgcttgt cgcaacttct cttctacttc ttcaaacatc
     7621 tctttacgca tcgagcgaaa tgcttcatgt atcagtgtcc gtgcatttcc gctatggaaa
     7681 atgatatcaa aagaagtcac ttgtagtgtt tcactgttta ttttttcagc catctgtttt
     7741 cctccttatt cgatcagttt gataaattta ctgctgaaat tctcaaaaat aggttcagct
     7801 aagatttcct gctgtacttt tagctggtca actaacctaa cgattgcttt agtcacgacc
     7861 gtaattcctt cattttcgac ataagaaggc gaaactaaaa aaacaaaatg aatttcttga
     7921 tattgatccc actgcatccc agaaggaatc aatgcaacgg ctacctttgt ttcgattcca
     7981 actggaacag cgggatgtgg gactgccact gtatcactga atatgatttg tcccatattt
     8041 tcacgttgct ggatctgctt tcgcatttct cttatatatc cttcttcttc atttatgctc
     8101 agcttttgca gcaaatcatg aagaatttct tcctttttcg gttcgtacgt ataagtcttg
     8161 aaaaattttt cagacaactg ttcggtgtaa atctgctttt tttgctgtag agaaaatgct
     8221 ggtatcggtc tatctgagga atgatagggt atagattctc caactacctt tcgtatttta
     8281 cgaacatcat cttcattcaa aaaaatactg acatgtaaaa ctggaatttt gaaaaacatt
     8341 gtcgaaagat caatagaaga aataatcagg tcgattcctt ttaaagttgt ttcattgatc
     8401 tcataatagc cttttacact taccactgta atatttttac cgaattcact gactacgcga
     8461 ttctttaata gttgggcact gccgtaaccc gttgcacaga taatcaatgc atgtagttta
     8521 tgcgcagctc tttctttttc caatgccgcc atgagatgca aagccaaata ggcccattca
     8581 tcttcattga tggtatagtt tttcaaagcc ggcatgttgc tgaaatgcct ttttactgcg
     8641 tcaaagactt tgggattttc tttctttatc tcctcagtca atgggttctc aatgcgaata
     8701 cctcggctca atcgaatcat catcggtgac aaatgatcga gtagtccatt gaaaagctgg
     8761 agatcctcta ctaatgaaaa atgaaattcc tgagacaatc ttttcagaac tttcgctaat
     8821 tcctttgata gttcctgatc attttgattt tctttgtgat tcgacttagc catgagatgt
     8881 aatgtcaaat aagcaatttc ttcttcggga aacgatacac ctgcgattac ctcaatacgt
     8941 tgaacgatcc gctgcgccac ttgatactct actcgcttag aaacttcttg ttcgatgcct
     9001 aattccttga tttcaaaccc ttctcggagc cgtttgatcc ccaaagccaa gtgtaaagtc
     9061 agattctgta tgataaaatc cgaaactttt aacttagctt ctcttgtttc atcgagaatg
     9121 ataatgacta gctcttcaaa actgatgtct gggaaaaaac ggctttgccc taaatattct
     9181 ttcaatgaat ttccataatg gtttccaaaa aaagtatcca tgataaaatg ccgtcgattt
     9241 ctttcttccc cttcgatcca tgtcccttga ccatgcttag aaacgatttt tagtgaatat
     9301 ggaatcaatc gctccttgat ttcttgcatg tcctttgtca aactggaacg actgatataa
     9361 agctcttctg ccaaatcatc cataaaaaga atggcatctt ccaaaagcaa ttggttcaat
     9421 atatactttt gtcgatcttc ggactcataa aatccttgcg gttctctagc atatccaggt
     9481 actatttctt gccgactcaa aaacaagtca aatgtcagtc tttgtaaaat atgcagttgg
     9541 tagccataac ctggtttagc aatgatctta cctccgtttt tttctactaa ctctttgatc
     9601 cgctgcaagt aattacggat tgtccgatca gataataaca attcagaagc taaacgttga
     9661 ctggtgacat actccttctt gtgatcaatc aaatatctca acagcaattt ttccttctgt
     9721 ttcattatca tccactccta ttccatcata tgttctcttc gatcacctct gctaacttct
     9781 ggatgcctgt agggattggg acatacgctt ggaaagggat actaacaaca ggctttccaa
     9841 cagattctcc taattttttg aatttatcca aaaacatcgt cgtttgcggg ctgatcagaa
     9901 ataaatcaaa atcactattc ttaatcatat tatcgccttc tgtagctgaa actgcatcta
     9961 cgagtacctc ttctcttttt tgcttaaaga aatccgttgt ctttgaggcc atcatcgagg
    10021 atgacattcc tgccgcacaa ataatcaatg cttttttcat aaatcaatca ctccttttat
    10081 ttttataaac caagcatatc ttgtaagcgt tttctattca aaatatatat ttccgtagca
    10141 ttgcggaaaa aatgtgtatc atatcttgaa taataatttc tggcaccgtg atatactagt
    10201 gatagaattt tttcaggagg ggatttagtg agtatcacag tgaatatttt gtatacaagt
    10261 acaaatggac aagccagaaa atttgcagaa gaaatggtcg aaagaaacat tgtcaaagcg
    10321 attcgtgcag aagaaggaaa cgagaaatat gactattact ttccactaga agatccagaa
    10381 tctcttttac tgatcgaccg ttggaaagat gaagaagcaa tcgaaaagca tcacaaatcc
    10441 gaaatgatgg ctcaaataaa cgaactccgt aaaaagtata aattaaaaat gaaagtcgaa
    10501 cgtttcactg atttaccgta aaaagataca catggaatga tagcaattta tcatccatgt
    10561 gtatcttcac tttttcctta ttttttattc gatctcatct aatgattcaa accgtgtttt
    10621 tctcttattt ttcggataag tatttcagtc accccaatat acatgattcc taaacatacc
    10681 ccaaggatat tctcaaaaat gcggatcgtt actgctcctg gaaccccgaa taaactggca
    10741 gtgattgcca aagcaccaaa agaattaaaa actgttttat atctataggt agaacaaaca
    10801 cctaaagcta gaccacctaa tataccgacc cagttaaaag gaataaattg gctgatacca
    10861 ataaataaaa cacatccaat caatgagcca atgattcggt ctactgctcg ttctttgaaa
    10921 ccaatcgaca tcaatccata gctagataca atagaagaaa atgcaaaccc tgcccacatc
    10981 aatcgctgaa aagggacata ctctcctgca acgatcaaaa gactaatgcc aaatgcatag
    11041 atgagcatcc aaattttttc ctgcgaatac atgcctttca aaaaaatctc tttaaacaag
    11101 cttttgtctt tgttcttttc tcgatgtttt ctgtaaagaa gaactgaaaa ccaacaaaaa
    11161 aacaaaaata ataagctact tgtttgtgta aagaaattct tgttcaacga atctttaggt
    11221 aacgaataaa caataaagag atatgaaaag ccgtacaaag aagcattccc cattttggga
    11281 tcactagcgg ttgccattaa tatgctgctt agtaaaataa aatgaatcaa catgcccata
    11341 actgaccatt tgatcagctg gacatacggt gctactaata gtgagaacat gacgattgct
    11401 aaacctatga tcgaatggga aatcttataa ccaaagtcaa caaaacgaat actcatcaac
    11461 atacagaata aaaccacagc gtatggttta tgcttcgatt caaataatgt tgtaattata
    11521 atgataaaac aaatggcaaa caatacgatg aataatgatc gtaaaaacaa tgaacaaata
    11581 tacatgcact tttctttttt agaagctgct tctcgtattt tttgttttaa aataaacgga
    11641 tctagttgta gagactgata aaaatccacc tgttgtcgtc ctttcttttt acttttattt
    11701 tttacagtca tctatgacag gttgtaaata ctatggaaca tcatatggcg ttgtcatttt
    11761 cttgtcaact atttttccta ttcatgtttt tataaaaaaa catatcgttt tcttgtatga
    11821 ttaaatcacc acaaaataaa caactgagag aacgatatgc tttatgaatg gttctatcaa
    11881 aaaaattctg agatcaatag acaaaaattt gatgattaca gaggtctctt acgaaacctt
    11941 acagaagaaa aaaacattga tcgttgctgc agttctcttc cctgctccat gtgtctgtag
    12001 aaattgtggt tctgctgtgg tagatggaaa taggaaatta atggtggtga aaaacaagaa
    12061 gaaggaaacc attgtccact ttgaataata taatcatatg ccaatggtta tacgcctaaa
    12121 aaagcagcgc tatacctgta aaaactgtcg aagccattgg aacgctcaaa gttattttat
    12181 ccgtccaagg cactcgattt caaatcatgt cagacataaa atcacttctt tactgactga
    12241 aaaagtgtcg ttatttttta tttcgaaaag ttgttaggta tctttgacaa ccgttattcg
    12301 cacattgaaa gagttgagaa gctatttacc aaagtcttcc aaaagaattc tgtcttgagt
    12361 gttgagggtt gtgaatttcg ttctcacgtt tccgttgagg ataaactgag ttttatttcc
    12421 gcggatggcg agacagaaaa attaatcgag aaaatttacc tacacaaaaa attcctcgat
    12481 taacaaacta tttccttagc tgcactaatc ctaaagaaat agaattctta gttacagaca
    12541 tgaacgccac ctactttcag ctcaccaaac atgttctacc aaatgcgaaa ttggtgattg
    12601 atcgattact tggtttttcc acgcctttca aggaggcgta acctttattc acgagttggt
    12661 tgaagctttc cgagacaaaa gacccgactt gtttttctcc ttattgacaa agagccatta
    12721 aaactagaga aaaaattaca actgatcgct gattatttgt agaaagaaaa tcaaagtatt
    12781 acccaataaa cttgagtcat taggcaaaaa acactgcgaa actcaagaat gattttcgca
    12841 gtgtttttgt atatttttta ttaactagta ttactacaaa ggtttatttt tcacttctca
    12901 acgcagcaca aaattgtctt tacttattta ctggttgggt tcaaccttct ctactccgat
    12961 actaatattt gtttgtatac cccttctaaa atcggtagtt tccattcaat tgatgggtac
    13021 tagttgattt taaaaactat gcttattcaa ttttttattt tagctcatat atttcgtttc
    13081 tttctaaatt ttatactgat gaaaacaagt agcacaccca atattgacaa tataaactgg
    13141 ctaatgatgg tttctccagt ttttggaaga tgcttatttc cactgcttgt tggattgttt
    13201 cctcttatat ctgagctatc agcaactttt ctattctctt ttgttaaaaa gatcgccact
    13261 tcttgttctt tttcgatctt gaaagttact ggtgtcgcat ccaattcata ccccgttggc
    13321 gcttgagttt ctaccagctg atatgctcct ggtgctaagc catctatcgc cagttttcca
    13381 tctgctcctg tggttagacc tgactgcaga acggttccat tggcatcttg taactcaaaa
    13441 acagctcctt gcaggacttc tccgctttgg tcatcgatct ttgtcaacac gacaccgcct
    13501 ggtgtaagtc gattttcttt cgtcaattct accaccgctg tctgacttct ttcgatctca
    13561 aactcgattg gtgtcgcatc caattcatac cccgttggcg cttgggtttc taccagctga
    13621 tacactcctg gtgctaagcc gtcaatcgcc agttttccat ctgctcctgt ggttagacct
    13681 gactgcagaa cggttccatt ggcatcttgt aactcaaaaa cagctccttg caggacttcc
    13741 ccgctttggt catcgatctt tgtcaacacg acaccgcctg gtgtaagtcg gtttgctttc
    13801 gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa actcgatcgg tgtcgcatct
    13861 aattcatacc ccgttggcgc ttgagtttct accagctgat atgctcctgg tgctaaaccg
    13921 tcaattgcca gttttccatc ttctcctgta gttagaccgg tttgtagcgt ttctccttct
    13981 cggttctgta actcaaaaac agctcctgca ggatttccc
//
LOCUS       DAGCXG010000064        13619 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGCXG010000064 DAGCXG010000000
VERSION     DAGCXG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.067"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            232..570
                     /locus_tag="MB022_001876"
     CDS             232..570
                     /locus_tag="MB022_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466870.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(775..1350)
                     /locus_tag="MB022_001877"
     CDS             complement(775..1350)
                     /locus_tag="MB022_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBT4466871.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1959..2540)
                     /locus_tag="MB022_001878"
     CDS             complement(1959..2540)
                     /locus_tag="MB022_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4466872.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2723..3349)
                     /locus_tag="MB022_001879"
     CDS             complement(2723..3349)
                     /locus_tag="MB022_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4466873.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3371..4702)
                     /locus_tag="MB022_001880"
     CDS             complement(3371..4702)
                     /locus_tag="MB022_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBT4466874.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4857..5258)
                     /locus_tag="MB022_001881"
     CDS             complement(4857..5258)
                     /locus_tag="MB022_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4466875.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5344..5550)
                     /locus_tag="MB022_001882"
     CDS             complement(5344..5550)
                     /locus_tag="MB022_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466876.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5645..7147)
                     /locus_tag="MB022_001883"
     CDS             complement(5645..7147)
                     /locus_tag="MB022_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBT4466877.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7165..7620)
                     /gene="argR"
                     /locus_tag="MB022_001884"
     CDS             complement(7165..7620)
                     /gene="argR"
                     /locus_tag="MB022_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4466878.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7718..8335)
                     /locus_tag="MB022_001885"
     CDS             complement(7718..8335)
                     /locus_tag="MB022_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBT4466879.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8556..9929
                     /locus_tag="MB022_001886"
     CDS             8556..9929
                     /locus_tag="MB022_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4466880.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10004..11116
                     /locus_tag="MB022_001887"
     CDS             10004..11116
                     /locus_tag="MB022_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBT4466881.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11188..12825)
                     /locus_tag="MB022_001888"
     CDS             complement(11188..12825)
                     /locus_tag="MB022_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBT4466882.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(12993..13601)
                     /locus_tag="MB022_001889"
     CDS             complement(12993..13601)
                     /locus_tag="MB022_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4466883.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3673 a         2862 c         2443 g         4641 t
ORIGIN      
        1 ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata
       61 gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca agaaacattg
      121 aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac cttcaatttt
      181 gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat catgcccgat
      241 tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg aaagttcacg
      301 aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa
      361 acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg tgagatcgta
      421 gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt ggataaatgg
      481 caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt aactttgaat
      541 tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat aaataaaaaa
      601 gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc gactgtgacc
      661 ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt ttttgggatc
      721 tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat tatgttatac
      781 cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc
      841 tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt catggattgg
      901 tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt tttcatcctg
      961 atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa acataaattt
     1021 gtctttttct tcattccact cataaaagcc actcattggg aaaacacaac gattttgcaa
     1081 aaatggttga tgaaaggtct ttttttcttc tactgtctct gcacgggcat taatcatcaa
     1141 ttgtcctttt ttaaatccgg taaatcccca cttcatgatt tccggagtga ggatgttttc
     1201 ttcctctttt cttaacgtca caacaagatt tgacggaaaa acttccccag ctgctaattc
     1261 tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa taattctgaa ggagctctga
     1321 ctctagatca aaaaaataac gaccacacat cctaatccct cccaatcttt ttttctatca
     1381 tacactgcaa caagaagttc gcaccaacaa aacgctttca atttttgtag aactaaaaaa
     1441 gttgtgacaa gtgacaaact ctccagtgaa tggtattctc ctgccaactc ctcggtcaca
     1501 agtcacaata ttcaacaaac atgagaagct gctttttgaa attgttcctt gtgactcgga
     1561 gtgaaacggc aggcttacga cctctgggta aacggtgttc aaaagcttgc atctgcggta
     1621 ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt
     1681 gatgcagggc agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg
     1741 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
     1801 tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc
     1861 gaaaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
     1921 ttcttgaagc tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt
     1981 aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact
     2041 ttcaacaata agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct
     2101 tcttcatcgg acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc
     2161 cattgattga ctaattctag tagcaacaga cggtattctt catccactgt actcaactca
     2221 ataatcaaat tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa
     2281 atccagtcca acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct
     2341 tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct
     2401 ttttttgaag aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata
     2461 tctcggattg acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag
     2521 atcttttcct ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata
     2581 cagtaaattc atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga
     2641 cacagccctc actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc
     2701 gaggacaaaa gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca
     2761 cttgatctgc aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag
     2821 caccaattgc atctgtcgct accacaacat tgaatccact ctctatcaat tcacgcatat
     2881 gggaatcgct acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt
     2941 ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga
     3001 tgatcgtatt ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt
     3061 cttccactga ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt
     3121 ctcctgcacc gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa
     3181 agagtttgta tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat
     3241 tgtatttttc taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact
     3301 cgacttgcgg gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc
     3361 cattcgttat ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga
     3421 tttgatctct caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga
     3481 tgacttcatg agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt
     3541 cgactaaaac tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc
     3601 ttgtcatata cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta
     3661 aacctacatt ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat
     3721 tcgttgttcg tttattctcg cccttcaact gatccataat gattcgataa tcatctagag
     3781 agatataagt gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat
     3841 ctgtacgaag gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat
     3901 tttctaatcc aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg
     3961 cttggattgt tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt
     4021 ctgctacttg atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt
     4081 ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa
     4141 gaacgacaac tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc
     4201 cagccccgat gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat
     4261 caatcaagta gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag
     4321 tattgatgaa aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga
     4381 tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc
     4441 tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag
     4501 cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc
     4561 cgatgttgat acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt
     4621 caccttttga agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa
     4681 tattttgata tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa
     4741 aaatatgata accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa
     4801 ccagacaaaa agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa
     4861 acaatcgtta cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca
     4921 tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct
     4981 aatactacac cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt
     5041 actgtggact ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa
     5101 caagaagcat aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct
     5161 ggaggagtga ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg
     5221 ccatggttga tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac
     5281 catttgctac ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc
     5341 tgcttattcg aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact
     5401 actcgtgaac aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc
     5461 ttttatatga ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac
     5521 tgttaaaaaa ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca
     5581 gcactcgatt taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac
     5641 tggtttattc tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt
     5701 ttactaacgc caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg
     5761 gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat
     5821 atcgaatacc ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc
     5881 gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta
     5941 atcggatttc ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga
     6001 taccttttct ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat
     6061 catccagaaa atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg
     6121 tatctttccc aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa
     6181 tataacgaac ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag
     6241 gggaattcgt cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg
     6301 cgatcaaatt atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca
     6361 catctgtatc gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac
     6421 tttccatcgc ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt
     6481 ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga
     6541 atccaccagc accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt
     6601 ctagtgttcc aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa
     6661 ggtctgcttt ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga
     6721 taaaagcaat atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat
     6781 aataataggg aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa
     6841 attgagtgga cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc
     6901 cttgttcttt cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt
     6961 catgaccaat cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta
     7021 tccagtttgc tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat
     7081 atccttcaac tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat
     7141 ctttcatata aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg
     7201 ctgtgcttct tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc
     7261 gacaacttca gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg
     7321 ttcagtcaat acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat
     7381 tgctgtacgc aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg
     7441 tactattttg ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat
     7501 cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt
     7561 cccaataggt tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat
     7621 attagacacc tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg
     7681 tattttttac ttttattttt tgttttcatt caggttgcta acctttttct attttaacac
     7741 gcaaaatcgt cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt
     7801 tgtgttcttt agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa
     7861 acgttgcagg ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt
     7921 ccatcttttt gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa
     7981 tggcttcttg tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag
     8041 aaaaatgttc ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc
     8101 cttccagtgg atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg
     8161 acatgcgtat tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct
     8221 gattcgggtc gccgatccct ctgatgctaa agaatggttt tttaggaacc tctagtattt
     8281 gcgggctttt tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc
     8341 ttcctccgcg aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct
     8401 aatcgtgttg acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca
     8461 gataacacgt ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact
     8521 ctcttttttt acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt
     8581 tagtacctgc agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg
     8641 ccatgaatgt aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc
     8701 aatgggtaac aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt
     8761 tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag
     8821 gtataatgat cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc
     8881 aaggtgcagc gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc
     8941 ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag
     9001 caattggccc cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc
     9061 tgtttcttgt gcccgttttg attctttctt tgattatcgg ctggtttgct attcccgaga
     9121 tggcagtaca aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt
     9181 ttagtggaag tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa
     9241 tcatcggcgg tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac
     9301 tgatccgact gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag
     9361 tttatcagtt tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc
     9421 aaggaagtag tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt
     9481 tgctttctcc tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg
     9541 ctgggctgat tctttctttg attggttggc tagcattatt cctactcata ggacacgctt
     9601 cagtgggtgt attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca
     9661 gcaatatgat gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg
     9721 caatgttcaa taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca
     9781 ttatcaatta cgtccagcat catacttccc ataactatga agtgtctact acattaggat
     9841 caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct
     9901 attatctgtt ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg
     9961 tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat
    10021 tatcggtggc gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa
    10081 tgttgcttta tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc
    10141 cccttggttg tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc
    10201 ttttttccct aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg
    10261 gacacttttg cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg
    10321 aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca
    10381 agcacttccg ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga
    10441 tggaaaagca tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat
    10501 ccaggcaaaa gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg
    10561 agaatacaca gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca
    10621 agaattaggc tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac
    10681 atcttataag atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat
    10741 tcctttcatg gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga
    10801 tttggctcct actagcgaag cttataaggg attgactaag agtacagaac agttcttgac
    10861 tgatccagat cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac
    10921 gtcagatttt tccccatttt ttggcgtact gccagatgat ccaagcttac tggctgctag
    10981 cggtctaggc tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt
    11041 cttaaatact ggtcattggt ctacggaaga ttatcaaaag gacatctcca cctatattac
    11101 gaaaaatcca tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat
    11161 ggattacttc ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact
    11221 ttcttttgct tcatctagca agagatcttt tcctatctga gcttcaatga atcgtaaacg
    11281 aatatcaagt cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa
    11341 atctggtata ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt
    11401 ttgttgcacg attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc
    11461 gaaatatcca gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa
    11521 agcttgatag tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac
    11581 tagctcatct agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa
    11641 tgttgcacca aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga
    11701 atcatctttc atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc
    11761 gggtgtcgca taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac
    11821 taggactttt tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga
    11881 agaaatggcc ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa
    11941 ggcagtagca aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac
    12001 agagtgacct tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc
    12061 aaatacagcc atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca
    12121 ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa
    12181 ttctccaaag taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat
    12241 gagtccattt ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc
    12301 ctcatcaaag gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa
    12361 attcagcaaa taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg
    12421 cacagaaaat tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg
    12481 actttgatac acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc
    12541 ctcaaagatc cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag
    12601 tgtcctaaca tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat
    12661 gatcgttcct ggactccctt ttgctagttg ataagctgtc ttcctgtcaa aaacttcatg
    12721 aacattatta ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt
    12781 tccacttgtg atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc
    12841 cctaaacaaa aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc
    12901 gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag
    12961 tttctcggtt tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat
    13021 gttttagcag catcccatga accataacga gatgttacat attgttctgc tacacgttct
    13081 tgattggcag ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg
    13141 taacgtccgt tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt
    13201 gcagagcttg aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt
    13261 tcaacaggtg caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt
    13321 tctgttgttt tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt
    13381 tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc
    13441 attgagttgt ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact
    13501 gtataaactt catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca
    13561 gcaaaagttg tacctaaaac aattttctta aatgaattca tattaaactc ctttttttc
//
LOCUS       DAGCXG010000065        13254 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGCXG010000065 DAGCXG010000000
VERSION     DAGCXG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.068"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            77..3304
                     /gene="fss3"
                     /locus_tag="MB022_001890"
     CDS             77..3304
                     /gene="fss3"
                     /locus_tag="MB022_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBT4466884.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3416..3730
                     /locus_tag="MB022_001891"
     CDS             3416..3730
                     /locus_tag="MB022_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4466885.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3743..4117
                     /locus_tag="MB022_001892"
     CDS             3743..4117
                     /locus_tag="MB022_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4466886.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4118..4471
                     /locus_tag="MB022_001893"
     CDS             4118..4471
                     /locus_tag="MB022_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBT4466887.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4540..5556
                     /locus_tag="MB022_001894"
     CDS             4540..5556
                     /locus_tag="MB022_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBT4466888.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6150..7157
                     /locus_tag="MB022_001895"
                     /pseudo
     CDS             6150..7157
                     /locus_tag="MB022_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7360..8679)
                     /locus_tag="MB022_001896"
     CDS             complement(7360..8679)
                     /locus_tag="MB022_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBT4466889.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            8836..9097
                     /locus_tag="MB022_001897"
                     /pseudo
     CDS             8836..9097
                     /locus_tag="MB022_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400658.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
     gene            9217..9708
                     /locus_tag="MB022_001898"
     CDS             9217..9708
                     /locus_tag="MB022_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4466890.1"
                     /translation="MKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKS
                     LQSLVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLP
                     KSLGGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQ
                     NIQ"
     gene            9834..10190
                     /locus_tag="MB022_001899"
     CDS             9834..10190
                     /locus_tag="MB022_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBT4466891.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            10235..11377
                     /gene="dcm"
                     /locus_tag="MB022_001900"
     CDS             10235..11377
                     /gene="dcm"
                     /locus_tag="MB022_001900"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBT4466892.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            11367..11656
                     /locus_tag="MB022_001901"
                     /pseudo
     CDS             11367..11656
                     /locus_tag="MB022_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11912..13096
                     /locus_tag="MB022_001902"
     CDS             11912..13096
                     /locus_tag="MB022_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4466893.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            13093..13206
                     /locus_tag="MB022_001903"
     CDS             13093..13206
                     /locus_tag="MB022_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBT4466894.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         4386 a         2293 c         2904 g         3671 t
ORIGIN      
        1 taaaaagact ttataaacaa agatgttcaa gcgagcatct tatttttttg tgcctaattt
       61 gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt
      121 gagtttggct gttcaatcaa ttgtaggagc aattgctcct acgattgctt ttgctgatga
      181 aattacccat ccacaaacgg taaccgtaga actagattta gcacatcaat atgctgttga
      241 aggaactttt agtgatggtc gtccaatgtc agaagtcact gttccgcatt atgcagtgta
      301 caacggcgtt aaacaagata ttttctgtgt tgaaccagga gtcccgattt acaatgagtt
      361 cactcctgga tatgagaaga acccgttacc agatatgtcc gataaagcca aattagtttc
      421 cgttttatgg aaaaaagtag gtacagatgc agatactcat attgtggcac agaagatgat
      481 ctggcaagaa gttaatgggt atacccttca ctcaataaaa cgatcagatg gtagtgccgt
      541 aaatatcgca gccattgagg caaaaatcaa tcaagccatt gccgattatc agaagaaacc
      601 aagcttccat aatagtacgg ctaaaacagt attagggcaa tcgactactg tgacggatac
      661 gaataatctg aatttgtcag agttcgatga ggttgtggaa aataccgcaa acattgatta
      721 tcgtgtgaat ggcaatcagt tgataatcag cccaaatgcc aattcaaaag aaagtggtgt
      781 gttgacgctt aagaaatctg ctggtaccgg aacacctgtc gcctataaaa aggctggcca
      841 acagacttta atggccgggg cgatcgataa gccgaacact tatacggtca aaattgatgt
      901 ggaaatggag ggttctttga agattaaaaa agttgataaa gaatcgggtg atattatacc
      961 aggaacggtt ttccatttag actttggaaa aactttaccc gcaaaagacg tgaccaccga
     1021 taaagaaggc attgcgacct tagatgagat tcctcatggt acaaaggtaa ccattactga
     1081 aaaatcggtg ccggcgcctt atacgattga taccacaccc atgactgcca caattaaagc
     1141 aggtgaaacc atttcagtga cttcaaaaaa tacgcgagaa aaaggtcaaa ttattctgga
     1201 taaaaccgga attgaaacag ggagtgatct ttggaacgat aattactctt tagcgggcaa
     1261 tacgtttgcc attcgcaagg atagcccaac tggtgaaatc gtccaagaaa tgaccacgga
     1321 tgaaaaaggt catgcagaaa caccaaaaga gattgccaat gcgttggaat tgggaaccta
     1381 ctatgtgact gaaaccaagg ctagtcatgg ctttgtgaat actttcaaac cagtaaaagt
     1441 cgaattgaat tatgtcaatc aaacagtggc tcttgttacc agtaacgtaa aagggcaaaa
     1501 ccaagaaatt actggtgaaa ccactttgac aaaagaagac aaagataccg gtcatgaaac
     1561 tcaagggaaa gctgagttta aaagagctga atacaccttg tttactgtta aggatggaaa
     1621 agcggtcaaa tggaccgaga actttaaacc agaattgttg aagggaacga aagcttctga
     1681 tgaaaccgta actttgactt tagatgaaaa gaatcaagtt gccgttaaac acttagcgat
     1741 taatgagtat tactggcaag aaaccaaagc gcctgaagga tataccttgg atgaaacaaa
     1801 gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa aatgctgtga ttactcgaga
     1861 tgttgcggca aaagaacaag tgattcgctt tggctttgat ttctttaaat ttgctggatc
     1921 aactactggc actgccgaaa ctggcttcaa tgatctaacc tttaaagtgt cgccattgga
     1981 aggaaccaat gaaatcaccg gtgccgaaga tgaagcgacc acagcttata acgagcaatt
     2041 agggtttgat ggttatggca agtttgaaaa tcttccttat ggggattatt tacttgaaga
     2101 agtagaagcc ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt ctacttttaa
     2161 agaaaataaa gaggattata ccaagagtga ttatatcttt actattaccg aaaaagggca
     2221 aaagcaaccg attaaaacgg taacggtccc ttatgggaat ctaaccaatg agaatttctc
     2281 agtcagtttg aaccgtttga tgctttatga tttgcctgag gaagaagata gcttaacttc
     2341 tcttgcgact tggaaagaag gagataaaga attgactact cttgattcta ccgagctagt
     2401 cgataaattg agctataatc tacatgaaat caaagaagac tggtatatcg tagctcaagc
     2461 catcgatgta gatgctacaa aagctgccca agaaaaagat aaaaaagcca aaccggtggc
     2521 gattgcggaa acaaccgcaa ctttagcgaa taaagagaaa acaggaactt gggagattct
     2581 gcataaatta actgtcgaac aagttttaga caagaccatt gtcttattca actatgtgta
     2641 tgaaagcaaa gaagcctatg aagctggtga tgagccggta gcgaaggatg ctagcttgaa
     2701 caatcaagcg caaactgtta attgtatggt ggaacgtcat gtttccatcc aaaccaaagc
     2761 gcacttagaa gaaggttctc agacctttac tcatggtgac gtggtagata tgtttgatga
     2821 tgtatccatt acccatgatg tactagatgg ctcaaaagaa gcttttgaaa caattctgta
     2881 cgctttattt ccagatggta caagtaaaga aatttggaaa tctggcaaga ttgattacga
     2941 agtgaatgac aaagaattta ccaaaaccgt acttgcggaa aaagtggata caagtaaata
     3001 tccagaaggt acaagtttta ccttcaaaga aattaactat gacaaagatg ggaatatcaa
     3061 tggaaaacac aatgaagatt tgaaagaaaa atctcaaact ttaacgccca aaaaagttcc
     3121 gacaacacca agtacaccag aacaatctga aatgccaact gttccaagtg gctctcaaga
     3181 atctagtcct acagtgaagg cattccctca aaccggggag aaaacttcca atgtgctact
     3241 gttcattggc tttagcttga tctttgcgac ggctggctat tatttttgga atcgtcggaa
     3301 ctaaggtgat aagatgaaac atgtaaatag tagaaaacaa tacgcgcgac cgcctcctaa
     3361 aaaagtggtc gcgctatttt tgtattcaaa actaacaatg aaaagaggaa aaaatatgga
     3421 attgaaattt gttgtaccag atatggtcga gacgttcgga aaactaacat acggtggtga
     3481 aggtgaagtt ctatcggaag ggtatggtcg aaatactaca attgttggtc gaagttacca
     3541 tctgttttca aataaacaac gggccgatga tattgaagtg gtggtagctg cggaagccgg
     3601 cgaaaaggat tttgaccaag atcaaccact aaaagctgta aatccccact taattgccaa
     3661 aggctatgaa attgagaatc gtgggtttac cgattacgtg ttgtatgttg atgatttagt
     3721 gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag attcaggatt
     3781 aacctttggc aagttgcgtt tttccgcatt acgtcgagaa gtacgcaagc aaaatgagga
     3841 cggaacggtt agtaacgagg taaaagaacg gacctataat ttgaaatcct ccgcgcaagg
     3901 tcgaatgatc caagtcagta ttccagcaaa tgtaccctta cgcgagtttg cttatgatgc
     3961 agaggtagag ctggtcaatc caatcgtgga tacggtcgcc aattatgttt ttcgggaagg
     4021 aaccaccgtt aattggttta tcaaggcaga tgatttagtc ttgaaacgac aactgaatca
     4081 aggaaaacct acgaaccaaa atgaaggaaa gaaataggtg aagaatatgg aaattaatgt
     4141 tgaacatatt ttagattgtc tcgatcaatt tggcaaaggt gagctaacgg aggaacaact
     4201 cactaacgca ttaacctatg atgaaaaaat gtttctgatt atgcatcaag ggttactgga
     4261 agtaggtaac gatgccaaag aagactttga tgtactggat tggttagggg aagaagagtc
     4321 tttctttatg gttgtcgagg taaatgaaag tttatgtcgg caggcagaat ctgttttgga
     4381 agaaatcgat gtggagatgc cggatgccat cgaaagattt ttaaaacaat tagtggaaac
     4441 gaaacaactt cccgtagtag taaatgacta gcataacttt ttgaaattgc ccaatgaaat
     4501 cctattgggc tttttcgact ggaggtggaa accatcaaaa tgtacaaagg acatcgcata
     4561 cgcgcaggag atcaacactt gatttatcac tttgttcttg gctggctaat ctttttgttc
     4621 attggttgga tgagcgtctt ttatttacaa gaacttagac aatttgatat ttctaaggta
     4681 tcattttcca ctagtgaaat tggctggtct atgaaggatg taatctgctt gctaggaagt
     4741 ctcgctcttt caggaagcat gatgttactt tatatacact tttttcagga tcattggcga
     4801 agtctttggc atcgacaaaa gttagctcgg atgattctag aaaaccattg gtatgaagta
     4861 aagcagactc aaggtgaagg ttttttcaaa gatttgaata gtagttggac caaagagagt
     4921 atcagttact tccccaaaat ttattatcga atgaaagatg gcttactttc tattcgcgtt
     4981 caaatttcac tgggaaaata tcaggatcag ctattgaagt tggaaaataa gttagaaagc
     5041 ggactgtatt gtgagttagt agaaaaagaa ctcaaagact cctatgtgga atacaccttg
     5101 ttatacgata tgattgccaa tcgaattggg atagaagaaa tagtggcaga aaatggggcg
     5161 ttgcgattga tgaaaaatca agtatgggcg tatgattcct tgccgcatat gttaattgct
     5221 ggcggcacag gtggtgggaa gacctatttc ttactcacca ttattcaagc actattgaag
     5281 tcagatgcag aattgtttat cctcgatccc aaaaatgcgg acctcgccga tttaggtacg
     5341 ataatgcctc acgtatactc ccaaaaagaa gaaatttctg aatgtgtgga agatttttac
     5401 gaacgcatga tagctcgtag taggtcaatg aaagaaatgc ccaactacaa aacaggagag
     5461 aattatgcgt atctcggact tccgcccaac tttttaatct ttgatgaata cgtggcttat
     5521 atgggtgcga atcgttttcc tacaagcatc gaatagatgt gaaatggaag cctattgaga
     5581 ttatcaatag tgacaactaa tgttttgaga gatggaatga aagccttcaa gttgagctga
     5641 aacatccccg ctaatactcc tacatgctct aatcaatgaa gtagattggt tttggtatga
     5701 agctaggtga agtcgtctga aatctgccta agtgcttgag cataaggctg actatagggc
     5761 gggcggctga aaaagataac tatggtaaga atgtctgaaa atgactgacg aatctgcgaa
     5821 tgtacgggtc taaaaggcta atcttattga aagataggaa catacggacg tatggtgaga
     5881 taccgacaag taaggttcca tgatatgaaa atcgggatag ctaacgaaga agctataatt
     5941 tcacaggctc atagtagata cctaaggtta tacatgatag ctaaaaacat tggaacgatg
     6001 taagcagaaa actatcaatt aaatgactac ttctagatag gtgcatataa gtaataacga
     6061 aattgctttg tttctgtgaa agtaagggca cagtaccgat gaagcgtcta acaaatgtgg
     6121 agggatagcc ctaagtctat ttaataacaa tgaatgttga aacagatgaa gttcaaggat
     6181 ctggtaagaa cgtgggaaaa tccgttatgg aggtgccacg cttgaacaca aaaatacgtt
     6241 actgggaata ttatgatcta caagaaacat tcgatcagct atttactcaa agtagaaacg
     6301 ggaaaaagtt ttatcaatta tatgagttaa tcatttctga aaataatatt cttttagcat
     6361 accgtactat caaggcaaac aaggggtctt cgacaccagg aacggattcc ttcacaattg
     6421 ataactataa ggaaatgaac caagcagagt ttatccatct tattctcagt catttagaaa
     6481 attacaaacc aaaatccata aagcgagtaa tgatacctaa acccaatggt gagaaacgcc
     6541 cattaggaat tccatgcatg attgatcgaa tgatccaaca gatgttcaag cagatactgg
     6601 agccaatatg tgaagctaag ttttatgaac atagctacgg tttcagacca ttgagaagtg
     6661 caaagcatgc tctaggtcgc attatgtatc tcataaacat cagtaaaatg cactatgcag
     6721 tagacattga tatcaaaggt ttctttgata atgtaaacca ccgtttactg ataaaacaat
     6781 tatggaatat aggaatttgt gacaaacaag tcttagctat tctttcaaaa tctctgaaat
     6841 cacccattca aggagaaggt atcccgagca aaggaacgat ccaaggaggt atcatttctc
     6901 cactattatc taatgttgtt ctaaacgact tagaccattg ggtctcaaag cagtggcata
     6961 cctttgaaac aaagtatccc tatactaaag gttacaataa atttcgtgct ctaagagata
     7021 caaatctaaa gcaaggctat attgtcaggt acgcagatga ctttaaaatc atgactaatg
     7081 attatccgac agcattaaaa tggttccacg ctgtcaagct ttacctaaaa gacagactta
     7141 aattagatat ttccaacctg aataattcat agctttaatt ctttggcaca ttttattgaa
     7201 atatgtatca ctaatatccc tatcaactga ttttgactaa aaagttactt caaacattca
     7261 ctgttcaaaa atattgagga ataaattttg ggtgtaaggc attttgagca tacttctccc
     7321 tggataactt cccagtaaaa aaatcgttag attgttggat tagatccatt gactggctcg
     7381 cctcaggcga gcaaagaccc tgtagaattc attctgatac acatgataac tagataattt
     7441 gagatagact cgtctacccg tttggactaa tttcccagca actttcagaa acttcaatcg
     7501 aatagaatga atggtcattc ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt
     7561 gactaagttg taagctatca agctgagcat cattcggaca tgattctcca aaaagcgagg
     7621 actgtctgtt ttgtcaaagt aaaagccagc tttcgcttct ttaatgaagt tctccattgt
     7681 gccacgtttg gcatagatag aaaagatggt atcaggagaa acattttctg atagattcgt
     7741 cacgataaat tcatgtcgaa agagtagttc gcccgcttca cgtgttgagc gtatacacac
     7801 gcgacgactt tgtgaccagg attgtgcttg ataagtggtc gagaagtact gaacttctcg
     7861 ttcttcccac ttttgattat cgccataaag tactgatttc tcagctattt gacctagtct
     7921 acgattattc ttcagtcgaa taacataatg actctttttt gattcacacg aatcatacac
     7981 accaggtgta gcgaacccgc tgtctccacg aaccaagatg tcagtgtttg gtagagagtg
     8041 attatagtgc tctaataaag gtgtgagaaa ctcctttacg ccttttgatg tatattgatt
     8101 tcctgaacgt agttcagctt ttaagaagtc accagtcaat ccgtcaaaag ctaccaatgg
     8161 atggtaacca taggtttggt agtgtgtatt ataattcgtt tgttcttggt gaccaaatgt
     8221 atctgaatgg gtcgaatcta aatcaatgat taattccgta tcattacgga tgaggcgtgc
     8281 tttatcgata agttcttggt tcaaagcttg aagttcatgg atattctcct cagatagtcg
     8341 atctaaaaat cgggaaagtg aagattgaga agcgagttct ttcctatcta aaacagcttt
     8401 aaacacagga tcttgtctca ggagattagc tgcagaatca gctgagtaac cagcaattaa
     8461 ttgcataatg aactgctcaa gtatggataa gttgtcatga gtaaaatacg ctcgttcgtc
     8521 ttcaatgtga atgtgttgct tagccagttc agaaaaacca aagatgttca tcagctcttt
     8581 cactaggacg agacccgaat cactcgataa ttgaccacct gtatgagaaa tagtgatatt
     8641 tgaattgaat tttacttggt ttttgtgtaa gctagtcatg aaaagaactc ctttcttttg
     8701 gttggtttag tcactttaac catagcagaa aggggttctt ttttcatcac ttaacgggtg
     8761 aagatgaaaa agtaattgta agctgccgtg aggcagtttc acatggtttt aattaaaagt
     8821 atgaattatt caggttccaa tgagaagtct aaaatcgtca atttgcgtaa atgtaagtcg
     8881 gaatttttag gattcgctat ttgcgtgaaa caaaaaggga aaaaatgggt ttgtaattca
     8941 cgtatttcta acaaaaaaaa ggatcagatt aaagaagaga ttaaacaaag aatcaaagat
     9001 attcaaaaga gtcctacagc acaaaatgct ctcctgttta atgctctcgt gctcggtgtt
     9061 cataattatt tcaggtatgc cactcatatt aatttagatt tgaataggat gaactacgtt
     9121 ctttcattca ctacttacaa ccgattatca aattgtagta caagatgtta ccccaacaaa
     9181 gcttccccaa cttacaaaaa gttttactca acaacgatga aaacgtataa agtagctgga
     9241 gtctatttgt accctctttg cgacgtatct acaaaaacaa tttacggatt taatacggag
     9301 gatacaccgt ttactccgtt tggtcgtcaa agattggagc acaaaagcct acagtctctt
     9361 gtgttccaag aattaagaaa attgatggaa tcgaaaatac cgaatagaac ggttgagtat
     9421 ttagatagta gaatatctcg ttactcaatg aaatcaggca agtgtgagat tacaaaacaa
     9481 tttttgccag caaaagccgt tcactgtcat cactatttgc caaaaagtct tggtggagac
     9541 gacaaattcg ataacttgag aattattcat aaggatatcc atcttctgat ccatacaaca
     9601 aataaaatga taatcgatca ctacgtgcat gaactgaaac tggtgccaga gcaaatagca
     9661 aaaatcaacc tttatcgaaa gatgtgtaat ctgcaaaaca ttcaatagaa aataaataga
     9721 tggaacgccg tatgaggtga aagtctcacg tacggtgtgg agcgggggaa aagatggaga
     9781 ttatatcaaa ttcttaccta tcgctataga tgttaacgac aaaagaaagt gcggtgattt
     9841 tgaataagct taaacaaatc gtgatgttag gccgtcaatc tggtttcttt ctgattctag
     9901 cttgtcaaag gccggatgcg aaatatctag gagatgggat tcgcgatcag tttaacttcc
     9961 gtgtggcttt gggtcgtatg agtgagcttg ggtattccat gatgtttgga gaagtcgata
    10021 aaaatttttt tatgaaacgc atcaaaggac gaggttatgt agatacaggg ggcagtgtga
    10081 ttagtgagtt ttatacgcca cttgtaccaa aagggtatga tttcttaagg gaaattagca
    10141 acattgttgg attaaccgtt catacggaaa gagaaaataa tagtgtgtag cctaggaata
    10201 tcaataatcc tgggcttttt tattgaggtg atttatgaca tttttagatt tattcgccgg
    10261 gattggtggc tttcgcttag gtatggaaca agccggtcac cagtgtatcg gtttttgtga
    10321 aattgatgaa tttgctcgga ggagttataa agcaatccat gatacaagga aggaggtgga
    10381 aatgcatgac atcacaagtg tatcagacga atttgttcga tccctcggac cagtggacat
    10441 cctttgcggc ggctttccgt gccaagcttt ttcaattgcg ggaaagcggc aaggattttc
    10501 cgatactcga ggtactttat tctttgaaat cgctcggttc gccactcttc tccaacctaa
    10561 gtttctattc cttgagaacg tccggggatt gctcaatcac gaaggagggg ctacgttcga
    10621 gacgatcctc cgaacgttgg atggattggg gtatgatgtg gaatggcaag tgcttaactc
    10681 aaaggcctac gttccccaaa gccgtgagcg gatcttcctt atcggacatt ctcgagatgc
    10741 atgtaccgaa caagtatttc ctatcattgg atcgtcttca acatctgatc aaaacatcag
    10801 aaacttggta aatatcaatc cttccaatcg aggaatgggt ggccaagtct atggttcaga
    10861 tagcgtagca ccaaccttga ccggtgatga aggaattaaa attgccttac cggtaaagga
    10921 tgggatttct gtagcgggta tgttgtcggg aaattttgaa caaggcaata ggatttatga
    10981 ggtaaccgga acagccccca ctttgtcaac gaaacaaggg ggaacgaaga ttatgattcg
    11041 gaaaaatgaa acagaatatc aagaggtgaa gccaggggac agtgtgaacc tggcttttcc
    11101 taattccaca agtcgccgag gtcgtctggg aaaacaaagt gtgcatacct tactaacagg
    11161 agaccaacaa gcggtagtca cggatcagta ccagattcga aagttaacac ctagggagtg
    11221 ctggcggctt cagggctttc cagattgggc ttttgatcga gcttctcaag tcaattcaga
    11281 tagtcagttg tataaacagg ccggaaactc agtaacagtt ccggtcattt ttgatattgc
    11341 tagaagatta aagggggtaa aagcatatga tttatgacga gttgattgga gaaatctact
    11401 gggtgattgg caagattcag gcagatccga aattggaaga agaactgtac cggctcaatt
    11461 ttgatattcg caaaaacggg gtgaaagtcc aggtgattcg tatgtagtgg acgaggaaac
    11521 cgatgctcga atggagctca atcaagtcat cggtgaattg gaacgaattg cggaccttgc
    11581 caaagaacct gatatccgcc aatacttgtt tgagataaaa gcagagttgg aaattgaggg
    11641 tatcaccgcg gagtaatagg gaaataaccc actgataggc actgaccgcg ggggatttgc
    11701 gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac ggcggcagcc ggccatgatg
    11761 tctcaccccc cgtatctaac agaggggtac aaaaacacaa aaaacaagca agtaaccata
    11821 aaaaatgctt gttgtatcaa ggatattaag taatttgtat aggtgttaac tcatttggaa
    11881 aagttaacac cttgaatttg gaggtgaaaa cttggcgcaa cgaaatttag attatcggtt
    11941 attaaaagaa cgacgcaacg aatatggcgt ttcacaaaat aaattagcta ctgcttgcgg
    12001 acttagccgc ccatacctaa atcagataga aaacggtggc gtaatcgcct ctacaaagac
    12061 catgagaaaa atttttgatc aactggaaag tttcaatccc gatttacctt taacgttact
    12121 atttgactat gtaaggattc gctttcccac aacggatgtg cggaaaatta ttcaagaaat
    12181 tctccatttg aaattcgagt atatgcttca tgaagattac gccttttact cctatcaaga
    12241 acaatatgtc atgggcgata ttgtagtgat gttatctcat gaagaagata aaggtgtcct
    12301 tttggaatta aaaggtcgtg gttgccggca gtttgaaact tttttactcg cccaaaagcg
    12361 tagctggtac gacttttttg aagattgtct aaaagccggt ggcgtgatga aacgcttgga
    12421 tttagcaatc aatgatcgag tggggctact ggatattcct gatttaacga aaaaatgtca
    12481 gaaggaagaa tgtatctcct tgtttcgtac cttcaaaagt taccgttccg gggaactgtt
    12541 gaaagctgat gaaaaggatg gtatgggcaa taccttatat attggcagcc tcaaaagcga
    12601 ggtttatttt tgtttgtacg aaaaagatta tgaacaatat atcaagttag ggattccatt
    12661 ggaccagacc gaaacgaaaa atcgttttga gattcggttg aaaaatgacc gtgcttatta
    12721 tgcgattcaa gatttgttaa aaggtcgtag cattgaaagt accacgtttt ccattatcaa
    12781 tcggtatttg cgatttgcgg ataaagtaga tggaaaacgg cgaaccaatt ggcccttgaa
    12841 tgaacaatgg ggtcgtttta ttggacggaa tcggaaagag attcaattaa cttctgaacc
    12901 gaaaccgtat accattgaac gaacattaaa ttggctaggt agacaagtcg cccctacgtg
    12961 gaaaatggct aaggagttgg atcgattgaa tcaaacaacg tatatccagg atatggtaca
    13021 gaatgcgcga ctctcggacc ggcataaaaa gattttggag caacaaagta tggcaatcga
    13081 aaacttgatt gtatgaaaag aggaattttg atggaaattg taaaagatat gttattggta
    13141 cttggtggca gtatgcttgg tgttaccgtg atgtgcttga tgaatgcaag tgcagcagcg
    13201 gattgagcaa tggaaaagaa aggaaagtga caaaatgaat ttcggacaaa attt
//
LOCUS       DAGCXG010000066        13209 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGCXG010000066 DAGCXG010000000
VERSION     DAGCXG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.069"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            144..1013
                     /locus_tag="MB022_001904"
     CDS             144..1013
                     /locus_tag="MB022_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBT4466895.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1222..2247
                     /locus_tag="MB022_001905"
     CDS             1222..2247
                     /locus_tag="MB022_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4466896.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2286..3236
                     /gene="manA"
                     /locus_tag="MB022_001906"
     CDS             2286..3236
                     /gene="manA"
                     /locus_tag="MB022_001906"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBT4466897.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3330..5033
                     /locus_tag="MB022_001907"
     CDS             3330..5033
                     /locus_tag="MB022_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4466898.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5035..7068
                     /locus_tag="MB022_001908"
     CDS             5035..7068
                     /locus_tag="MB022_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4466899.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7079..7519
                     /locus_tag="MB022_001909"
     CDS             7079..7519
                     /locus_tag="MB022_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4466900.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7719..8846)
                     /locus_tag="MB022_001910"
     CDS             complement(7719..8846)
                     /locus_tag="MB022_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466901.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8849..9526)
                     /locus_tag="MB022_001911"
     CDS             complement(8849..9526)
                     /locus_tag="MB022_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466902.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9790..11556
                     /locus_tag="MB022_001912"
     CDS             9790..11556
                     /locus_tag="MB022_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBT4466903.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11571..12008
                     /locus_tag="MB022_001913"
     CDS             11571..12008
                     /locus_tag="MB022_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4466904.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12021..13172
                     /locus_tag="MB022_001914"
     CDS             12021..13172
                     /locus_tag="MB022_001914"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBT4466905.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3697 t
ORIGIN      
        1 ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt
       61 tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct
      121 tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa
      181 aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg
      241 ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg
      301 gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca
      361 aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt
      421 gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg
      481 ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga
      541 acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag
      601 aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta aatgttacta
      661 acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag
      721 aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg
      781 gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga
      841 tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct
      901 tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt
      961 tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc
     1021 tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct
     1081 agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt
     1141 ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg
     1201 atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg
     1261 gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca
     1321 tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg
     1381 tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta
     1441 gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat
     1501 cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca
     1561 aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt
     1621 gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg
     1681 ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca
     1741 ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt tcccacagaa
     1801 caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg
     1861 gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc
     1921 caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt
     1981 gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac
     2041 ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg
     2101 tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat
     2161 cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg
     2221 aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt
     2281 gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa
     2341 gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg
     2401 cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa
     2461 gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt
     2521 ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag
     2581 atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca
     2641 tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac
     2701 tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa
     2761 aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga
     2821 ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag
     2881 atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc
     2941 ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca
     3001 cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt
     3061 tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg
     3121 ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt
     3181 ctgagtgggc ggtccaaggt gaactagcaa tcattgcgac tgaaccaggc aaatagttcc
     3241 cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc
     3301 catcaataaa tagaagtatg gtgaaaaaga tggaatacaa atggaaagca aacaacagca
     3361 gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc
     3421 caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg
     3481 gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg cttttaccca
     3541 tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg
     3601 caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga
     3661 ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc cacgtgaaat
     3721 tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta
     3781 tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg
     3841 cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag
     3901 aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag
     3961 gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg
     4021 gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa
     4081 atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat
     4141 ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt
     4201 tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg
     4261 tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca
     4321 gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag
     4381 agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca
     4441 tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga
     4501 aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata
     4561 accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag
     4621 tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac
     4681 gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca
     4741 cagaacaaaa aaagtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct
     4801 cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg
     4861 ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac
     4921 aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg
     4981 aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat
     5041 ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct
     5101 atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat
     5161 aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca
     5221 tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa
     5281 agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg
     5341 acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt
     5401 tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg
     5461 aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta
     5521 ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg
     5581 aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg
     5641 gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg
     5701 atgctgacat tgacgatgga gcgtgtgact aaaaatcatt tactgacaaa tgagacatat
     5761 aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc acggatcgct
     5821 tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat
     5881 gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct
     5941 tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa
     6001 gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt
     6061 ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa
     6121 gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa
     6181 gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa
     6241 agcaatatca gtattgcagg attttcgcca agtggattag cttctactag catgttggaa
     6301 ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat
     6361 ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca
     6421 ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag
     6481 ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc
     6541 aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc
     6601 aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa
     6661 acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt
     6721 catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg
     6781 gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg
     6841 aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta
     6901 gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt
     6961 ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc
     7021 gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat
     7081 gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc
     7141 gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca
     7201 atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc
     7261 acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc
     7321 tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt
     7381 ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga
     7441 catcgaccaa gtaatggcgc tcagtgatgc gaaaaccatt gaagaagtcc aaatgatttt
     7501 tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat
     7561 aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg
     7621 gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt
     7681 gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag
     7741 atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt caagatacca
     7801 ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca
     7861 ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat ccatccaatc
     7921 gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact
     7981 agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac
     8041 aacaacagga acagaaagct ctggaaaaga tactcataca tcggaaggat tcgtatctcc
     8101 tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt gattattact
     8161 agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg
     8221 gtaaaacgga tgctatgttt ctttaccata ctatccggta atagattgac tagagagatt
     8281 acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa
     8341 atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagggaga
     8401 tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc
     8461 aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt
     8521 cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct
     8581 gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca
     8641 aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag
     8701 ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat
     8761 gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga
     8821 ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta
     8881 aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt
     8941 tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct
     9001 ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt
     9061 tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga
     9121 catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct
     9181 tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag
     9241 tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga
     9301 aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat
     9361 aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg
     9421 gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac
     9481 gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa
     9541 tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta
     9601 ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga agttttctga
     9661 taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa
     9721 agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg
     9781 agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa
     9841 aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg
     9901 ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc tttggctacc
     9961 atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa
    10021 gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc
    10081 ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc
    10141 atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc
    10201 aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt
    10261 ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct
    10321 gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat
    10381 aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga
    10441 aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct
    10501 tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac
    10561 tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc
    10621 ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga
    10681 ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat
    10741 tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct
    10801 gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa
    10861 gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca
    10921 ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg
    10981 ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat
    11041 caagcaatca gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta
    11101 caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg
    11161 aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt
    11221 tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa
    11281 gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc
    11341 aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat
    11401 ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag
    11461 atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac
    11521 gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt
    11581 tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct
    11641 attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc gatgccatgg
    11701 tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa
    11761 cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg
    11821 gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag
    11881 gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg
    11941 acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa
    12001 ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag
    12061 gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc
    12121 acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat
    12181 caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa
    12241 aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct
    12301 taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca
    12361 caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa
    12421 aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa
    12481 atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag
    12541 tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat
    12601 taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg
    12661 tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg
    12721 atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag
    12781 gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa
    12841 aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga
    12901 caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag
    12961 aacataactt gacttgccca catttgacgg caacaatggg gattattttt aattactatg
    13021 atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac
    13081 aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa
    13141 acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac
    13201 gaaattttt
//
LOCUS       DAGCXG010000067        13169 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGCXG010000067 DAGCXG010000000
VERSION     DAGCXG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.070"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..371)
                     /locus_tag="MB022_001915"
     CDS             complement(<1..371)
                     /locus_tag="MB022_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466906.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRIN"
     gene            complement(382..1050)
                     /locus_tag="MB022_001916"
     CDS             complement(382..1050)
                     /locus_tag="MB022_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4466907.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1181..1765)
                     /locus_tag="MB022_001917"
     CDS             complement(1181..1765)
                     /locus_tag="MB022_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4466908.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2096..2329)
                     /locus_tag="MB022_001918"
     CDS             complement(2096..2329)
                     /locus_tag="MB022_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBT4466909.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2344..3291)
                     /locus_tag="MB022_001919"
     CDS             complement(2344..3291)
                     /locus_tag="MB022_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4466910.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3291..4133)
                     /locus_tag="MB022_001920"
     CDS             complement(3291..4133)
                     /locus_tag="MB022_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4466911.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4266..4991)
                     /locus_tag="MB022_001921"
     CDS             complement(4266..4991)
                     /locus_tag="MB022_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4466912.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5452..5766)
                     /locus_tag="MB022_001922"
     CDS             complement(5452..5766)
                     /locus_tag="MB022_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4466913.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5782..7377)
                     /locus_tag="MB022_001923"
     CDS             complement(5782..7377)
                     /locus_tag="MB022_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBT4466914.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7400..8290)
                     /gene="iolE"
                     /locus_tag="MB022_001924"
     CDS             complement(7400..8290)
                     /gene="iolE"
                     /locus_tag="MB022_001924"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HBT4466915.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8307..9332)
                     /gene="iolG"
                     /locus_tag="MB022_001925"
     CDS             complement(8307..9332)
                     /gene="iolG"
                     /locus_tag="MB022_001925"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBT4466916.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9356..10363)
                     /locus_tag="MB022_001926"
     CDS             complement(9356..10363)
                     /locus_tag="MB022_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4466917.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10476..11636)
                     /locus_tag="MB022_001927"
     CDS             complement(10476..11636)
                     /locus_tag="MB022_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBT4466918.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11712..13166)
                     /locus_tag="MB022_001928"
     CDS             complement(11712..13166)
                     /locus_tag="MB022_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBT4466919.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPASMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         3928 a         2860 c         2166 g         4215 t
ORIGIN      
        1 tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact
       61 aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca
      121 atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt
      181 tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt
      241 gtggatttcc cactcccaga agaaccagta atcaatgtaa gactaccttt ttttattgaa
      301 aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta
      361 aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa
      421 caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg
      481 agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg
      541 tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg
      601 aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa
      661 gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa
      721 agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac
      781 cggcaacatt cgaaatacaa tatataagga tgtcccaaaa aatatagtaa aaaactctat
      841 cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata
      901 aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc
      961 cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt
     1021 aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact
     1081 gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa
     1141 gcataactga gaacaattct caatgcaagc ttttttttaa ttaactcagt aattcaatcc
     1201 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
     1261 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
     1321 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
     1381 aacagactgt cttttttgtc acaaatgatg taattttttc aacaatatct tgatatttgg
     1441 ttccagctga ttggaataaa accaaatcga atatacgttt attctcaaaa atataattaa
     1501 taaatggatc taattcattg aagttctctc tccaactttt ctctatatct atattttcta
     1561 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
     1621 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
     1681 tagttacgcc tgcggtcaag cacagttgcc ttatcttagt ttgctgaaat cctacttttt
     1741 caaaatggct tagtccactt tctattaatt tttggtgagt tgtaatataa tctctggttt
     1801 ctatgggtac ttcctcctaa taagatgaat taactatatt atcttacaca ggacttgaag
     1861 tttcaatcgt taacgtctat tactgaactc attccttaga gctaaactaa tgagaaagag
     1921 caaaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
     1981 ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat taggataagt
     2041 cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa gccttttact
     2101 taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta
     2161 tatctaaata gtcctcatat ctaagtttag tcagcatctc acttacagat gctggactaa
     2221 cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct
     2281 gaattgctag taaataaatt agtatagtag ttgttttaat ttgtaccata cctaccctcc
     2341 aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta
     2401 actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg
     2461 aatattggta tcttttgata ctgttttcat tggtctatcg tccacactgc tttctacgaa
     2521 taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt
     2581 tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga
     2641 gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc
     2701 aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt
     2761 ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt caatattttt
     2821 agcgtaaata ataccgtttt ctaaatttaa ccaagcatgg ggatcttcct tccctttctc
     2881 actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt
     2941 ttcctctttg ttcgcatttt ttactaattt tgtaaaccaa gcatttcctc cagtttccaa
     3001 gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa
     3061 aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc
     3121 ttttgctata ttttcagtaa tatctgctaa aatcgaatta gtaactacta cctttaattt
     3181 ttcgttcgaa gccactgtat ctttgtcttt actatttgtt gaacatgccc cgaatataaa
     3241 aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca tcataacttt
     3301 ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag
     3361 aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata accaatgaac
     3421 aaacctagca atgatgagca cgctccgact gtcgccgaaa taaatatcat agtttttaaa
     3481 ctgtttgcat ataaatatgc tgtagcagct ggtgtaatta acattgcaac aattaatata
     3541 gttcccacac tttgcatagc tgttactgct actaaagtta atagaatcat taatagataa
     3601 tgataaaaac tgacattcat ccccattgct tttgccatta atggatcaaa agaagtgatt
     3661 aataattctt taaaaaatag aacaactgta accaacacta aaacagaaac gccaattgta
     3721 atccacttat caatatcttg tacagctaaa atatttccaa ataagatatg aaataaatcc
     3781 gttgagctat ttgcaactcc tataaggata actcctaatg ctaaaaaaga actgaaagta
     3841 ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt aataatgata
     3901 gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa aatataagaa
     3961 attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga cattcctctt
     4021 aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga tgtaattaga
     4081 gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag catattattc
     4141 cccttgtata ttagagttag ggttttaacc ttccggtttt gattggataa ccctatgaac
     4201 catcgttatt ttaatttggt attaactcat ttcttttgag ataatatatt tattggattg
     4261 agaggctaag ccgttccccc ctctccaata aatatagtat ctccataggc tttttttaaa
     4321 ttctcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga
     4381 ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt
     4441 tttccctgat gtttcaattc ttttaacgta tccataatta ttcgttcact aactaagtct
     4501 atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct
     4561 aaaagtacac gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt
     4621 cccatatcga ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt
     4681 tgaaaaactt tcattccggc atatgttccc atagatacac attcttttac ttttattgga
     4741 aaagtaaaat caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg
     4801 atatcttcat tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta
     4861 agcaatgtag attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt
     4921 atttttaaag aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc
     4981 acttctaaca taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca
     5041 aaaattatat tttagtcaac cctaaatttt ataatttaga tttttttctg ttgttgaaga
     5101 ttaggatgtc aaaattattt gaaactactc gtatagtaaa tagtaagtaa ttttcaaaac
     5161 acgaagaaaa acaaaaagaa aaaaggaaac gccgaagcat ttcctttcag tgatttaatg
     5221 agaacaacac gatttgactt agagtctcaa aaagtcgaat aggtacactc atttaagtgt
     5281 ctccattcga ctttttgggg atgagctggc tattgaggtc cgcttaccct attgaataaa
     5341 tacaagattg ggtatttcta ataagtccgc ccttcatatt ggggtgatcg gggcgaacat
     5401 gccatctttt catataccac ctcctcgaca ctcaataatc aaagattctc gttaatcttc
     5461 ttcgatgctt gcaatgatgt tttcaatatt catataacga aaatcttctt gtttgatata
     5521 aacgatctga gcggtacatt tttccttttt gatgatttct tttacttttt cctcagaacg
     5581 tgcgacctgc ggcgttaaaa gtaaaacgtc atagtcttta taattcagtt tttcttcaac
     5641 gtttcggatc gttgcataat ctatctcata cgatagattt ttggctctga tcgtttcaag
     5701 catttttgat acgagcagcg acgcactgat tccggtctcg caaataatca atagatcctt
     5761 atgcataata ccctcctgtt tttagcctgc aaagccaatt ggtgaaaatg ccacataagt
     5821 taaaataaca atgactacca atgctatagc agtcaccttg gtgtatttcc aaggctcgat
     5881 atcgaccttt gtatcgaatg agttcaacac aaattctcca tcaggtttca atttggaaac
     5941 caccatcaaa atgataatgt ccagaacgaa taatgccgtc aaaacataaa ggaaatggat
     6001 attttggaaa agcatttttg atagtgcata caagacgata tgcacggcaa tcgtcgtttt
     6061 tgccccaaaa gcagttgctt tttttgaata aaaggcaaag agaatgatca cgagcagcgg
     6121 catgttgtat agtccattga attcttgaac gaactgatac aatccagccg gtgcaaaaga
     6181 aatgaatggc gctacgataa tggagataat ccccacaacg accgttactg ttttaccagc
     6241 acgagcgatt tgcttatcat ttgctttctt attgataagc ggtttataaa aatccagtga
     6301 aaacaaggtc gatgtcgcgt tcaaagctcc cgcaaatgat gataagatcg cgccaaagat
     6361 aacagcggcg aaaataccaa agatcatttt aggcaatact tcactgacta aaaaaggata
     6421 cgcgttatct ggatttgaca ttaaactatc gccaaaaata ttgcgagcga cgatccctgg
     6481 aaagacaagg aataacgcac ctaagatttt gaagacgccg actaggaaag cgcccttttg
     6541 tgcctcagct aaattcttgc cggacaatgc tttttggacg atcatttgat tggtacacca
     6601 gaaatacaga ttgttaaaca gcatccctgt aaatagtgtc ggccatggaa caacagccga
     6661 gtcgatcgat cccacagagt taagcagcca tggcgtgttt accacaacgt gttcgatccc
     6721 gccaacaaag ctgccgtcac caagcttcat taagcctaaa attgggacca gcagaccaca
     6781 agccagcaga ccgactccat aaatggagtc actattggca cttaacgaca agccgccgat
     6841 cagcagatag aggatgccga caataccaat tgccatcgct acaattatga tcgcgacgat
     6901 cggctggacg cccagcagct catcaatatg gaagatctta ttgaacacca gcgatccaga
     6961 ataaagaacc acgggtaaga aagaagtcat ataggtcaca ataaataaga tcgaaataat
     7021 tcgtttggta accgtatcat aacgaatctc gatgaaatct gagattgtat tgatgccgta
     7081 tttaaaatat tttggcaaga atatcacagc caaagctacg atcgcaatcg tagcagttac
     7141 ttcccaggcc attacttcca tccctgcatg ataactttga ccattttgtc cgactatttg
     7201 ttcggtggac aggttcgtca tgatgatcgt gccggcgatt ggcaaagcag ttaagctccg
     7261 ccctcccatg aagaaccctt ctgatccaga agtatcgaca cttctggatc tagaataggc
     7321 aaataaccat acacagatga caatcactag gaaactgatt atagaaaaaa gattcatcgt
     7381 ctagctcctt ttcaatagat taaagaccgg tgacctctcg aatgtagctt ctggctttga
     7441 ctgcatattc aaatggattg gctttagccg ggtcctgttc tgcttcaact acgatccagc
     7501 cttcatagtc gctggcatgg attgctgtcc agattggagt gaaatcaatc atcccatcac
     7561 caggaaccgt gaagacgcct ttcttcacgc cgtttaagaa gctgtctttt tccttttcta
     7621 cttcttcagc cgtttcttga cgaatatctt tgaaatggat gtgtttgata cgatcttgat
     7681 gacggttgta aaccgcgatt ggattttcac cagaaaagac caaatgtccc gtatcgaaca
     7741 ataatttaac tttgtctgga tcagtcattt ccattaaacg atcgatttct tccgtcgttt
     7801 gaacgccggt acccatgtga tgatggtaaa cgatctccat atccttttcg tgggccaaat
     7861 cccctaattt ttctaatcct tccgccagtt ttttccactc ttcatcagtg aaaactggtt
     7921 tatcatcaaa gactggcgct gtcataatcc cttggatact atgaccctgc tcagctacta
     7981 cgatgacttt agcgcccatc gcatgtaaga aatcccgatg tgctttgaag gcttcggcag
     8041 tttcttcaaa aggtttagtt gtcagaaacg cactgaacca tgcactggca acctttaagc
     8101 cgcgaatatt caaatattgt tccaacacct ctggatcttt tggatatttg ttgccaatct
     8161 ctgttccggt gaacccagca agggccattt cactgataca ttgttcaaag gtattttcac
     8221 taccaagctc gggcatatca tcattggtcc acgcgattgg tgcgatggct aattgaattt
     8281 gtttggtcat attctcacac tcctgcttat ttttttagtt gtactaaact cttcgtatcg
     8341 aatgattctt gacaagccaa cgcgatctta gttgattgca atccatcttc tgcattaacc
     8401 gctggttgac gtttttcttg aatacatgag acaaattctt gggcttcacg aatgaaggct
     8461 tctctgaatc gttctgggaa gttttgtgaa catggacgga tgatcccttg atcgttcatg
     8521 atcgtcacaa gatttttctc aggcgcttgc gctacccgca gcattccttt ggttcccatg
     8581 atctctgttt cgacatgata gccatgggct gcgtttctgc cggcaactag taaagctatt
     8641 gttttatcgc tcaggctcat cattgcggct ccagtttcaa gctcaccagc ctgatccaat
     8701 tctggatacg ccgcattttt cccaatcgcc cacacttgat cgacttcttg gccggtaaac
     8761 cagcgaacca aatcaatatc atggatcgcc atatctaaga acaagccgcc gctgtcgtta
     8821 ttcatggcaa atttgacaaa gctttccaag ccttcgctcg gatcgatccc ataacaacga
     8881 atcagtgtga tttcgccgat ttcgccatct tctaccattt tcttcgcata ctgatacgac
     8941 tcatcatagc ggcgcatgaa gcctaacatg aagacctgtt caggatgctc ttcgatgact
     9001 ttttgggtct ttttaatatc ttcaacttca aggcctaatg gtttttcagt aaagacatgc
     9061 ttgccatttt ccatcgccaa gcggacttgg tcacagtgaa agcctgatgg tgaaacaata
     9121 aagactgcat cgatcacgga acttttgacc atttcttcat agctggaaaa agtatgcttg
     9181 acgcctaatt tcttctgtgc gaatgctaat tctgtttcaa ctaaactgca cgcagccgta
     9241 agctcacaac aactgacacc tgttgctaaa ttttgtgcat gtacttttcc taatcttcct
     9301 aaaccgacga tgcctactct aatgttgttc atgaaatacc tccgttttct gagaattact
     9361 cattgtatag tgctggtcgt tcatccattt gaatcattac ttttgttttt gtttcacgag
     9421 actcatgaca agccatcatg gtgatcgctg ctacataccc atcccaagcg gtaggaccga
     9481 caaccgcatc atttttgatc gaatcgatcc acagcttgaa ttcataatca taggcatcca
     9541 aaaagcgggt ctgccaatct ggtgaaacag gtgtgtaatt tttttcttcc gatttaattc
     9601 gtgtataggc tgggtccgtc attcctactt ctgccgtttc gaaaaccaca tcacatttaa
     9661 tatcgtagcc aaagcggcaa ttcacaaaga tttccaaatc aatcgttaca ccggattttg
     9721 tttgaaagat cagcagctgc ggatcatgca aatcggcatg ggagttgctg gtttttttcg
     9781 gcaagatcat ttgaacagat tcataatctt catttagcaa ccagcgaagc acatcaatct
     9841 catgaatcgc ggtgttgctg atcgccatcg gtgttgtgta gttttcatct gcgctaggat
     9901 ttctatgggc acaatgcatc attaatgctt ctccgtattg cttcgattcg attgcttctt
     9961 tcagttcaat gtagccgcgg tcatagcggc gcatgaagcc aacctgaacc aatttcttcc
    10021 cataagcaac ttccgcatca acgatccggc ggcagcctgc tgcatccatc gctaatggct
    10081 tttcgcagaa aacatatttc ccatgcttga ttgcttctaa cacatactgc tcatgggtcg
    10141 ggtcccaaga agtcacaaca acgacatcca catcttcaga agcgatcaat gcttctccgg
    10201 tttcaaagaa tttagcatcg acacgtttgg caacctcttt agcagctttt acattgatat
    10261 ctgagactgc tgtcacataa gccccttgtg ttttctgatt gatcctgtcg atatgctcga
    10321 tgccgattgc acctgttccg ataacgccta cttttactct caattactta tccccctgtt
    10381 ctgttgttta agcacacata gtatgcgctt acaaaattat tcctgtgtag cttttattct
    10441 taaaaggtag agccctctct ttgctccgcc ttttactacc cataagcttg tacgtataat
    10501 tccttaattt gactatgact aggcaccttt ttattatttt gcggacttcc gctggtcaac
    10561 gcatccgcgg ccattttgtc taaggctgcc ataaacgctt gctgatcaat accataatct
    10621 cgaatcgttg ggatcgacag ctgctcacag agctcttgga tttttttaat cagataatcc
    10681 gcagcctctt ccagcgataa atcttcggct gccggataaa taaatctggc gatcgtcgct
    10741 aactcagtct gaatagcatc ctttgagaat tcaaaaaaga ccggcaataa catcgcattt
    10801 gatatcccat gaggaatgtg aaaaagtgcc cccaccggtc gactcattcc atgaatcaat
    10861 gtgactgacg cattcgaaaa ggcaatcccc gcctccgttg aagctaagct catttgttct
    10921 ctggcttcag tgtcgtcgct attttcatat gcctgagaaa gcgtgttcaa aattttcttc
    10981 atcgcttcca gtgaaaatag ttgcgttact tcattggctt tgactgaaac ataagattcc
    11041 aaagcgtgac atagcgcatc gactcccgtt gccgcggtta cttttggtgg tacagttttc
    11101 gtaaaatcag agtcaacgat tgagatctgt ggcatgaaaa cgagatcctt catcatcatt
    11161 ttgactgctg tttttgtatt ggtaataact gccacatcgg ttacttccga accagttcca
    11221 gcagttgttg acactgcaat cagcggaatg cccgcttgca gctgatcaaa atttcttgca
    11281 tgaatcgctt cgagacttaa ttgattagca gccaaaacgg ctaccacctt ggctgtatcc
    11341 agacagctgc cgccaccaaa agcaatgata tgatcacatt gatgcttctg aaaaagcttc
    11401 agcgcatcat aaacattttc ttgcgtcggt tcggcttgaa tgtctgtata ctccgctaat
    11461 tgatcatctg tttttcccaa cagctccttg atcttttgaa gatgcccggt ctgttccata
    11521 accgtatcac taatgattaa cggcttgttg cctagttgtt ctgattcttc cataagaaat
    11581 tggattgttc cgccgccaat tcttaaattg gcaggcattt tcagttgaat attcacagaa
    11641 aaaacctctc tctggcttat agcctataac agaatcctcg aagctcttta acagagcttc
    11701 gaagcaaatc tttaaatctc actttgcgca taacgtgctg taacaacttt ccgatgggta
    11761 taaaactcaa cactgtcttt gccattggca tgcaaatcac caaagaaaga gtgcttccag
    11821 cctgaaaagg caaaccaagc catcgatgca ggcaccccaa gattgacccc cagcatccca
    11881 gcatcaacat tttctctgaa gtagcgaatc gctgatgcgt tgttggtata cagacacgaa
    11941 ccattcgcaa attcagattc attcgtcagc tcaacggctg ccatcaaatc ttttacccgc
    12001 acgattgatg cgaccggggc aaaaatctca tcctgccaga tcttcatatc catcgtgaca
    12061 ttatcaaaaa tcgttggtcc aacaaaatag cctttttctg gaatgttttc gcgaccatct
    12121 agtaccagtt tcgctccttc atcgacccct ttttggatat agtcaaaggt ccgctgctgt
    12181 gcatcttttc gaatcactgg tcctaaaaag accccttcgt ccaagccatt gccgatcttg
    12241 atcgcttttg ccgcagcctt aaatttttca acaaactgat ccgcgatttc ctcttccaaa
    12301 acaatgacgg aggctgccat gcagcgttca ccagcagaac caaaagctcc gccaataagg
    12361 tccttgactg cccgatcaat attggcatct ttcagcacaa caacatgatt tttcgctcca
    12421 gtcaacgctt gtacccgttt tagatttttt gttccttcac ggtaaacgta ttcaccgaca
    12481 ttttttgatc ctacaaagct gatgcctttt accagtggat cttgcagcaa tgtgttgaca
    12541 acatcgtggg caccatagac aacattaaaa acaccgctag gaaagcctgc tttttcaacc
    12601 agctcaacaa ttttctccat caacaatggt gttttttctg aaggcttcag caccaccgta
    12661 tttcctgccg ccagagccat cgggaagatc cagaagggca ccatcattgg aaaattaaac
    12721 ggtgtgatcc ctccgattac tccgattggg taacgataat tcgtgatctc aatatcagtc
    12781 gccgctgttg ataaagaatc tcccatcatt aaattggtga ttcccgctgc gtgctccaca
    12841 ttttcgattc ctcgttgaac ttctcccagt gactcggaca atgatttccc attttccagc
    12901 gtgatcaaac gcgccaattc acttttatga tctgttaaaa gctgatgcag cttgaacaga
    12961 taacgtccgc gaacaggaac aggaactttt ttccaagttt gaaacgcagc atcggcagct
    13021 ttaactgctt cggctgtctc tgctttgcta gacaacggta cattcgccag cgcttcttct
    13081 gtagccggat tcattacttc ttcaaactct gttgagcgac tagcgaccca acgaccatca
    13141 atataatttc ttaaaaatct tgtcattta
//
LOCUS       DAGCXG010000068        13150 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGCXG010000068 DAGCXG010000000
VERSION     DAGCXG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.071"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            628..1161
                     /locus_tag="MB022_001929"
     CDS             628..1161
                     /locus_tag="MB022_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBT4466920.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1651..2175)
                     /locus_tag="MB022_001930"
     CDS             complement(1651..2175)
                     /locus_tag="MB022_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466921.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="MB022_001931"
     CDS             complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="MB022_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBT4466922.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2764..3744)
                     /gene="pta"
                     /locus_tag="MB022_001932"
     CDS             complement(2764..3744)
                     /gene="pta"
                     /locus_tag="MB022_001932"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBT4466923.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3897..4577)
                     /locus_tag="MB022_001933"
     CDS             complement(3897..4577)
                     /locus_tag="MB022_001933"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4466924.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4703..5572
                     /locus_tag="MB022_001934"
     CDS             4703..5572
                     /locus_tag="MB022_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4466925.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5598..6113
                     /locus_tag="MB022_001935"
     CDS             5598..6113
                     /locus_tag="MB022_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466926.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6160..6753)
                     /locus_tag="MB022_001936"
     CDS             complement(6160..6753)
                     /locus_tag="MB022_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4466927.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6778..6957)
                     /locus_tag="MB022_001937"
     CDS             complement(6778..6957)
                     /locus_tag="MB022_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466928.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(7034..7543)
                     /locus_tag="MB022_001938"
     CDS             complement(7034..7543)
                     /locus_tag="MB022_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466929.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7639..8019)
                     /locus_tag="MB022_001939"
     CDS             complement(7639..8019)
                     /locus_tag="MB022_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4466930.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8349..9098
                     /locus_tag="MB022_001940"
     CDS             8349..9098
                     /locus_tag="MB022_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBT4466931.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9282..10559)
                     /locus_tag="MB022_001941"
     CDS             complement(9282..10559)
                     /locus_tag="MB022_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466932.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="MB022_001942"
     CDS             complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="MB022_001942"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBT4466933.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11481..12425)
                     /locus_tag="MB022_001943"
     CDS             complement(11481..12425)
                     /locus_tag="MB022_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBT4466934.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12427..13113)
                     /locus_tag="MB022_001944"
     CDS             complement(12427..13113)
                     /locus_tag="MB022_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBT4466935.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3800 a         2742 c         2337 g         4271 t
ORIGIN      
        1 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
       61 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
      121 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
      181 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
      241 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
      301 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
      361 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
      421 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
      481 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
      541 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
      601 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
      661 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
      721 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
      781 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
      841 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
      901 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
      961 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     1021 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     1081 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     1141 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     1201 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     1261 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     1321 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     1381 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca agaggttgtg
     1441 aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc ctcatatttt
     1501 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt caaaaaaaat
     1561 ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga
     1621 tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca ttttatatac
     1681 atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt cgatttggaa
     1741 gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt caagctccaa
     1801 tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca taagagtgcc
     1861 tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca cgtgagacaa
     1921 acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt catagattgc
     1981 aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt ctaaggtttc
     2041 tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct tattaacttg
     2101 tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca agatgatccc
     2161 aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg aaggtttcac
     2221 tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta tcactttttt
     2281 ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt ccccattcaa
     2341 caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct ccgctagctg
     2401 ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat tctctaataa
     2461 tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca attcctttcg
     2521 tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca cctggcattg
     2581 cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat agttcgatca
     2641 tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat gaagtttttt
     2701 taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt tcgcgtgtat
     2761 ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata catcttcttc
     2821 atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat
     2881 tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg actgcaattc
     2941 tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt tcagttgtgc
     3001 aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt attgtggcgc
     3061 tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg atttcgctga
     3121 gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca attctgctgt
     3181 ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca cgttgatcgc
     3241 acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca tcgctccgct
     3301 tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc
     3361 agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg taagcatcgt
     3421 accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg cctttccatt
     3481 tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat cagggctgat
     3541 gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta tttcttcttc
     3601 gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa
     3661 aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa caattttaaa
     3721 tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat gtattatcat
     3781 acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa atgctggaaa
     3841 aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct gttttattac
     3901 actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag cgcatcattc
     3961 gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg actgggatgt
     4021 ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc ttgtgccggt
     4081 tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc aatgatcgtg
     4141 tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc ccgaacggta
     4201 agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt tccatgttga
     4261 acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa cgatggcggg
     4321 atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc tccatgataa
     4381 ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc caatgcttca
     4441 tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt taaaaattct
     4501 cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg ccaactgtta
     4561 tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac attttcttca
     4621 atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta ggaacaagaa
     4681 gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg acggcacatt
     4741 attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt ttgcaaatga
     4801 tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc gcgcagcttt
     4861 agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg tatttgataa
     4921 agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct ctgttttaga
     4981 tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc tttattctga
     5041 aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt tacctcacct
     5101 tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc acatcacata
     5161 cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa aaattatttg
     5221 ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt ctcttttagg
     5281 cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca
     5341 aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag aagaagtcat
     5401 gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag tcagtttcgc
     5461 aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg acacaaacct
     5521 tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt agttttatct
     5581 agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt ggacgtgcaa
     5641 ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt ggtcgctggg
     5701 gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt gctcacatca
     5761 agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg
     5821 gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt agacttcact
     5881 ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc aatcagcaaa
     5941 tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg gatgtctcaa
     6001 atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac tggctctacc
     6061 gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taacttaagt taaaaagatg
     6121 tacctgaaat gcggaaatac gcatttcagg tacatctttt tagatattct tttgcatatg
     6181 atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat ggcgttcgta
     6241 taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg agcgattagc
     6301 tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa
     6361 atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt ctttatcaat
     6421 gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca gtggctgatc
     6481 gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt taccgtctat
     6541 ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga ttgcttcttc
     6601 taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt
     6661 tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg caaaacgaat
     6721 catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc aaaatcgtta
     6781 ttttaaaaaa tcttcaatct tttcagtaat gaaataacca tgagtaacga ttcttgccgc
     6841 ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt cgactacatt
     6901 attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt tttccatgtt
     6961 atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa aaaagagaag
     7021 caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca tctattaatc
     7081 ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc acttttgctt
     7141 ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg aactcttccg
     7201 ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt acggttgttt
     7261 tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg tattcatatg
     7321 ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca gatatgttat
     7381 gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc cgatattcct
     7441 ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc
     7501 ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat tctcctgttt
     7561 ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa
     7621 gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg gatcaaagaa
     7681 aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatccctaa
     7741 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
     7801 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
     7861 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
     7921 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
     7981 aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc tattcttcgt
     8041 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
     8101 agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga
     8161 cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc aagaaaacaa
     8221 acagaaaatt atcataacac aaattattta taattgaaag actagttttt tttcaagaaa
     8281 agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt cttaaggagt
     8341 cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc tggccgcaac
     8401 aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt gtcaatagtc
     8461 ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta ttgtgtattt
     8521 tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct gcgatttgtt
     8581 ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag attgcttata
     8641 tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat atggcttcaa
     8701 aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact ttccttgtct
     8761 atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg tgggtagatg
     8821 ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt tacaacaacc
     8881 aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat atttcattta
     8941 cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg
     9001 tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa
     9061 tggaagaaga atactacaac gcacaacaac acgcataaaa aaactgagcg tgggacaaaa
     9121 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaatc agaagcaact
     9181 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
     9241 tatttcttgg agttaaacgc ttctgtccca gcctctattt tttatagtaa ttccttcaga
     9301 tactttcttg cctcatcatt gaaacgcagt gattcttcta acttctgata atattccaac
     9361 gcttgtttct gagcttcagt aaaacgataa tgttttcctt tccatttgtc gattgtctct
     9421 tgcacatttc cagattggat atctttcact actagttgcc atttttcttt tgagactgta
     9481 cggtctttct tatcagcaga ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa
     9541 ccatttctga ctatccgagg aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa
     9601 tattgcttcc aaataaatag atagattttt ggttggatga atcgttggtt tttcgcaaaa
     9661 tattccatct gagcagtgac ttctggtgtt cgttgacgtt ttgtctttct caacacggaa
     9721 ataaatgttt caaaataagc attaggatgt actggcactt cttcatatac gatggattgc
     9781 caatctttac gtaaaaatgc gcgatagttg atcaagaatt cttctacatc ctttactaat
     9841 tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc gcttttttga ctgcccattg
     9901 atgtgttcac ttattttctt gatttcattt tccaatgctt gataatccgc tatgtaaatc
     9961 ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta attttatatc cggtaaatat
    10021 ggctgtttca tccgacgatt ttggtacaag acaaaacagt atttttgcca tagtccttca
    10081 ggaaaatcga tattcttctg cttaagccat aataattcgt ctaaggccag atcaactttt
    10141 gtcattccct gatggatcga agctgccact gtcggagaga catgcaggtg atcggcaaag
    10201 tggttgatgc ctagttttag gatctttccg gtttttggcg atttaacgat gtattgcact
    10261 tttaattctc taccacactc acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac
    10321 ggattgatct tgaagtcaga aaacacccaa tctgttgcag ctaaataatg ttcttccata
    10381 aaacgagagc tgattagata tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt
    10441 aattttgcat agatctccca acgttgactg cttgtgtatt gtttagaaat tttgatggga
    10501 tagcttttgc tgatttcttt tggaaaccat tcttcaaaga cggattgcca ttcttccacg
    10561 gctgtttctt tcctttctaa ttacaaaaag ctggctacta acataacgtt agcaaccagc
    10621 cagtgtttct tgctaaatta gtgagttgtt ccggcatttc cggaaatatc ttctttattt
    10681 tctactgctg cttcaactgc ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa
    10741 ggcgtaccca ctggacgatc gatcacttga tcgggtgaga atggcacgtg gatgaaccca
    10801 cctcgaattg ccgggaattg tgtttcgatc aaatgaagca aacgatacat gatatcattg
    10861 cagacaaatg ttcctgctgt ataagagatg tacgctggta gaccatgatc ttgtacattt
    10921 tttgtcattg ctttgattgg caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa
    10981 gccgtatcga atggctgatt tccttcatta tcagggattc ttgcttctgc tagattgatt
    11041 gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt gtccgacaca gatcacgaca
    11101 tctggttggt gctgttggat ggcttctttg actacttgac tgcttttttc gaatacggtc
    11161 ggtacttcca ctttgatgat ttcagcaccg ctgatttcat ctggcatttt ttttactgct
    11221 tcataggctg gattcacttt gtccccacca aatggatcaa aacctgttac tagtactttc
    11281 atctgttgtt cctccgtccg attgatttaa catatggtta tcttttatca taccattttc
    11341 cttgaacagc gtaaataaag gaaacaatag tttaaataac ataacagatc aaacttaata
    11401 tattcttata aaaaaatagt gttccactac aatgttagaa gaggttgcga caattattct
    11461 gtcacaaccc ctctcttcta ttatttaaat agaatcatat aagcaatttg gaaaactagc
    11521 ataaataacg caataaacaa ctggtttttg atcactccgt atttgtcttt catctccagc
    11581 atcgctacag gcacgatatt gaagttagca gccatcggtg tcagcagggt cccacaaaaa
    11641 ccgcaagtca atgcaaccat tcctattaac gctggattcg ctccatgact aaatacgaat
    11701 ggtgcaccga tcccaaccgt catcacggtg atggccgcaa atgcattccc catgattacc
    11761 gtaaatatca ccatgccaag agcgtaaatg atgatgccga tgttcacatt tccttctgga
    11821 acgatcgtac ctactgcatt ggaaatcact gtcccaactc cagcgcttgt aaataccgca
    11881 cctagagaag ctaaaagcat tggcaacatg cttaacggcc ctactgtgcc cagcatatct
    11941 gctgcatcat ctaaaaatgt acttggtcga ttatctcgag aaaagaacat caagattagc
    12001 atggctgcaa aaacaccgac gcccacgccg actaaagcac ctaaattggt aaatagagca
    12061 aacaagatcg caaagacacc gatactcaaa gctggaataa aaattttcat tcctagtttg
    12121 tctgacattt tttccatata ttcttttgat ggaagccggc tttctccttt gctgactttc
    12181 ttagcaattg ctggtatggt catcgcaaag atcaagccac cgttcacgat tgccggaagc
    12241 catcttcctc ctgcaataac tacccctaag accgtccaga aaaaggctga cccataacga
    12301 tgaggattgg ttttatctgt taaatttctt actccagtgt aaatcaatac gagtcccatc
    12361 acgatataaa ggatctccag taatttatcg cctaataaaa tatcaggatt tgtaaaaaag
    12421 tccatcttat tgcccctttc ctggataata tttcttcgct aatttctttt ctttaagata
    12481 gaagtagaca cttgccacaa ctaaagccgt gatagccact ggaacttcca ctaaagcaag
    12541 ctgtcccaat gttacgtcat atcctagatc tttcagtgtt gattggacta gcaacgcccc
    12601 tgctcctccg atgaatagta cttggccaaa gaaccagcag acattttcca tagctgaagc
    12661 catacctttt agttcttctt catgcttagg atggacttct aacccttttg attcgatggt
    12721 ccccaatgcc atcggtaaaa tgattggacg aacaaatcca gcaactccac caaaactcac
    12781 gttaaaagcc gcaaagatca ttcgaaagat cccataaata tctacaatca taccagcaga
    12841 tactttcttg aatcgcttga tcaaggaagc cgctgattct ttcagaccat ttctttcaag
    12901 cgttcctgtt gccaacataa tgatgataaa aatcgccatc ccgcgattgt ttacaaaact
    12961 tgtacctaaa agttctaaca ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac
    13021 taaagcaacc attacgatca atatcgagtc taatttaaat gcaaacccga gaataacaat
    13081 caagacgcct aaaaggctaa gataattttc catatatttt cctccataaa atgacaatat
    13141 ttttttaatt
//
LOCUS       DAGCXG010000069        13127 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGCXG010000069 DAGCXG010000000
VERSION     DAGCXG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.072"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            162..1103
                     /locus_tag="MB022_001945"
     CDS             162..1103
                     /locus_tag="MB022_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBT4466936.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="MB022_001946"
     CDS             1172..1903
                     /locus_tag="MB022_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBT4466937.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="MB022_001947"
     CDS             2047..4227
                     /locus_tag="MB022_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBT4466938.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="MB022_001948"
     CDS             4247..4729
                     /locus_tag="MB022_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBT4466939.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="MB022_001949"
     CDS             4745..5506
                     /locus_tag="MB022_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBT4466940.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="MB022_001950"
     CDS             5637..6971
                     /locus_tag="MB022_001950"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBT4466941.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="MB022_001951"
     CDS             7278..7763
                     /locus_tag="MB022_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBT4466942.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="MB022_001952"
     CDS             7756..8439
                     /locus_tag="MB022_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBT4466943.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="MB022_001953"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="MB022_001953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBT4466944.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="MB022_001954"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="MB022_001954"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBT4466945.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="MB022_001955"
     CDS             12055..12684
                     /locus_tag="MB022_001955"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBT4466946.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="MB022_001956"
     CDS             12736..13068
                     /locus_tag="MB022_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4466947.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4430 a         2321 c         2895 g         3481 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaag
//
LOCUS       DAGCXG010000070        13113 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGCXG010000070 DAGCXG010000000
VERSION     DAGCXG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.073"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            77..1816
                     /gene="yfcC"
                     /locus_tag="MB022_001957"
     CDS             77..1816
                     /gene="yfcC"
                     /locus_tag="MB022_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBT4466948.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1973..3157
                     /gene="iadA"
                     /locus_tag="MB022_001958"
     CDS             1973..3157
                     /gene="iadA"
                     /locus_tag="MB022_001958"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBT4466949.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3268..3471
                     /locus_tag="MB022_001959"
     CDS             3268..3471
                     /locus_tag="MB022_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4466950.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3572..4234)
                     /locus_tag="MB022_001960"
     CDS             complement(3572..4234)
                     /locus_tag="MB022_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4466951.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4247..5749)
                     /locus_tag="MB022_001961"
     CDS             complement(4247..5749)
                     /locus_tag="MB022_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4466952.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6297..7976
                     /locus_tag="MB022_001962"
     CDS             6297..7976
                     /locus_tag="MB022_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4466953.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8103..8582
                     /locus_tag="MB022_001963"
     CDS             8103..8582
                     /locus_tag="MB022_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBT4466954.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8801..9481
                     /locus_tag="MB022_001964"
     CDS             8801..9481
                     /locus_tag="MB022_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBT4466955.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9489..10820
                     /locus_tag="MB022_001965"
     CDS             9489..10820
                     /locus_tag="MB022_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4466956.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10835..11797
                     /locus_tag="MB022_001966"
     CDS             10835..11797
                     /locus_tag="MB022_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBT4466957.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11908..12246
                     /locus_tag="MB022_001967"
     CDS             11908..12246
                     /locus_tag="MB022_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBT4466958.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12325..13032
                     /locus_tag="MB022_001968"
     CDS             12325..13032
                     /locus_tag="MB022_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBT4466959.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4164 a         2318 c         2878 g         3753 t
ORIGIN      
        1 tttttactgt ataattcatt tgattatcca ttttttattc atcaaaatta ctgaaaaaag
       61 aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac
      121 taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct ttgcatggat
      181 actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat acaaagatgc
      241 aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt atgcttacga
      301 gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc cagcagaacg
      361 tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa
      421 tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt acgatcagta
      481 cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa tatggggaac
      541 agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag tatctggaga
      601 taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag gtgcttttgg
      661 tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca gtaaaactaa
      721 gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg gtggcgctac
      781 attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat ttgtcattgc
      841 gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa
      901 tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg ctggaatccc
      961 cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg cattagccgc
     1021 tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta
     1081 caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa ataaaaaatt
     1141 catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct ggatcgtttg
     1201 gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct ttgtgatggg
     1261 attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag tcaatgacat
     1321 cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg tagtaggtat
     1381 ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt cagctttgaa
     1441 tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg
     1501 ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt caggacaagc
     1561 agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat ccagacaagt
     1621 agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac ccgtatcagc
     1681 cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg caagattcca
     1741 aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga ttgccattgc
     1801 aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa cagtagataa
     1861 ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct atcgtttgga
     1921 ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat gtatgaaaat
     1981 cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg tactgattgc
     2041 cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag gagtggaagt
     2101 agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact gtcatttcca
     2161 tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag tcacattgag
     2221 ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag acggagaagg
     2281 tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag gaatctccac
     2341 ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa
     2401 agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat ccgatcaccg
     2461 ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg
     2521 catgttatcc ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa aaagaaagct
     2581 agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat ttttgcctac
     2641 tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca aacgtggtgg
     2701 cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag atggtgaagt
     2761 tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag ataatttcac
     2821 gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc atttcctagg
     2881 gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg tgcaaaaaga
     2941 aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac gtatattgaa
     3001 attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta tcttggataa
     3061 agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc aagaaaaaga
     3121 agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa ctatcttttt
     3181 tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga aggtagatca
     3241 ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc taaagatatg
     3301 aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga cgacaagagc
     3361 aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat
     3421 ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata aaaaccactt
     3481 attactaatt gaatcaagca aataaaaaat ccgaaccata tctgcgagac agatatggtt
     3541 cggatttttt atttgatcat tatcctgttt tttaaacttc gattttttga aattttttat
     3601 tttttagttc aaagacttca tcacagcttt ttgctacttc aatctcatga gtcacgatga
     3661 taatacagcg attttgttca tgggcgattt cttggaaaag tttgacgata tcttgtgaag
     3721 ttgtttcatc tagatttcca gtaggctcat ccgctacgat tagttgatgt tcacagcaga
     3781 tagcacgaac gatagcgact cgctgctgct gacctcccga aagtttggtc acctgttttt
     3841 ttgctaatat ttcatcaatt cctacacgag ccagtcgatc taaagcaact tgtcgataat
     3901 tctcttgctt ggtcttagtg atttccattg ctgctatgac gttgtctaga gccgataaat
     3961 aaggaagcaa gttgtaagct tggaaaataa tcgagacatc atttttacga taacttctta
     4021 acccgatttt gttcagtgaa cgatcttgat agtatacctt tccttctttt ggtgcatcta
     4081 gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc acttgctcct aaaatagcgt
     4141 acatttttcc agcttcgaaa ttcagttcta catctttata aagcgcatct tgcggatctt
     4201 gataccaaaa tcccaattgt tctgtccgta acatcaaatt cctcctttag tttaatagga
     4261 tttttcttgg gtttaatctt aatatcccta tagaagctaa aaagacagaa aacagactga
     4321 ttccaaatgc tactccggct aatagtccca attgtgcagg tttgacattg atttccaatt
     4381 cgctgacttg atcagaaaca gcaaatggat tattcgtggt tccttgttga ccatcgcgtt
     4441 gaccgccttg ttcttgtctg ttacctggca tttgtccacc gccaggagct tgctggtttt
     4501 gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc cacggcattc gcgacgacgt
     4561 ttccgctaag cgaagcaact cctaatgcaa gaatcataca aatagccact tcggtaaaca
     4621 attggagaat gattttactt ctggattctc ctaaagatag caagacgcct aactcatgcc
     4681 gacgctcccg aatggtaatc ataatgatca gagtaagtat gataataccg gctacagcca
     4741 ctagtaaaac aacattttta gaaaaacttg ccacgttgtt taaaggttcc aacattgact
     4801 gatacatgga atcatttgat tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt
     4861 ctttgagaaa ctcatccatt ttatctggat cagatagagt gtaaatagca gaatctatcg
     4921 tgttatcgtc agatgtccca tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat
     4981 tcgacggatt catgaagttg aatttcatac ccatactgcc gcttgtttcg ctggattcat
     5041 aaattccttt gatcgtcatt tcataagtgg tatcttcatc ttcagtactc gtgattgtga
     5101 aagtatctcc gactgccagg tcatttgttt ctgctagagt agaatcaatg actacattgt
     5161 ttgtgccttt gtcgctgtca tctattcctt caccatccgt gatttttgct gttccatcag
     5221 aaaaattact attttgtgca gttgctgaca caccactgat ttgaaagtct gcttgagtca
     5281 tttgcggatt cccgcccatt cctccagcag gttcaccgcc tgcatcagta gtttctgaac
     5341 tgtcttcact tgaatcagaa ctagtgatgg cagttattcc atcttttgcc atagctgagg
     5401 cggaactttc aaaggaataa ctctttacat tatctaattc agctattttt tccgcgtccg
     5461 ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg acgttcttca gtggtagaac
     5521 cgcttgaaga agtttgttcc tgttttttaa atgaagcttc tcgattggca cttaaagtca
     5581 ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg
     5641 tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac
     5701 gtccccattt tacttttgta cttttccatg cacgtttcat aaaattcata ttttgcatcc
     5761 ctcctagtag atgcatttga taatagacaa cctatcttaa agctagctta aatcttatgc
     5821 tgtttgcaca ccatcaacat gaggttctgt atatataaaa agtcactata gttattgcaa
     5881 aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat gaaagaggaa
     5941 tagcaaaaga aatcctagat gctacagagg gatttttacg tgcatagttg ttctaacaat
     6001 tgctgatact agcgatttag tttatttgtt gtataataat ggggaacagc tgaatactta
     6061 tataagtatt ctggctatag cgtttcgcaa aagatccatt ttgttaatga agtatatcca
     6121 aaataaaatg cccatccaac tgtcagaacg cggatgtgct ggtaattttc ctgctgattc
     6181 tccataagtt gagaaatagt gattttgcat atgcttcatt ggaatggctc atatatttag
     6241 ataaagaaga aaatcgacat ctttaaactt ggatgtcata agaagaggtg aagtttgtga
     6301 gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg
     6361 cagaagtggg cgaggatatt tttattttag attgtgggct aaaatatcct gaaaatgagt
     6421 tgttggggat cgacgtcgta atccctgatt ttacgtattt agaagaaaac attgatcgtg
     6481 tagctggtgt ctttttaaca catggacatg cagatgcgat cggggcactt ccttacttgt
     6541 tgtcaaaaat ttctgtacca gtattcggga cagagttgac gatcgagtta gcaaaattga
     6601 atgtcagtcg taatgaagca gccaaaggat ttaaggattt ccatgtggta gatgagcata
     6661 cagaaataga ctttggtgaa acagtagtga gctttttccg taccactcat acgataccag
     6721 attcagtggg gatcaatctg aaaacaaaag aaggtagtat cgtttatacg ggagatttta
     6781 aatttgacca aacggctatc ccaatgtatc aaacagatta tgcacgattg gcggaaatcg
     6841 gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg
     6901 tagcttctga agcccaaata gcagatgaag tatttgatac gatcaaatat tgggaagggc
     6961 gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag
     7021 cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga attattcgca
     7081 cagcgatgcg tttagaaaaa cttcaattac caagtgaaga tctgcttgtt acgttgaaag
     7141 acatgaaaaa ctatgatcca gaagaactga tcattttaga aactggccga atgggtgaac
     7201 ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag attgaagaag
     7261 gagatttggt ttatatcact actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa
     7321 cggaagatat catttatcga gctggtggaa cagtgaagtt gatctctgag aatatgcgcg
     7381 tttctggcca tgccaatcct aatgacttgc aattaatgat caatttgatg aaaccaacat
     7441 attttgtgcc agttcaagga gagtatcgtg aattagctgc tcatgcagat ttggctcatg
     7501 cagtagggat gccatacaaa aatatctata ttactggacg cggagacgtg ctggaatata
     7561 agaacaagcg tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg attgatggat
     7621 taggcatagg tgatatcgga aacattgttt tacgtgatcg aaaaatcttg tcagaagacg
     7681 ggattttcgt agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc
     7741 aaattacttc acggggattc gtttacgtaa aagctagccg tgatctgatg aaagaaagca
     7801 gcaatatgat tgaaacaatc gttgagaaac atttagaaag caatgatttt gagtggagca
     7861 aattaaagca agacatccga gatcaattag gccgttactt atttgaacaa acaaaacgcc
     7921 gcccagtgat cttgccagtc attatggaag ctactcaacg tcgaaataaa aaataaacaa
     7981 aaagagtaag atgaacctct ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa
     8041 ataatttttg aatacagcat ccctcgcttt ataataaacg atagaataaa gggagtgaat
     8101 caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc agctatcaac
     8161 cacttgattt tagtcatgtg gtagaagaat ctttcacaga agaactacaa aaaagaggga
     8221 ttgatccagc tttgatatgt aaaactctcg tagtgaaagg aaacaaaact ggggtgatca
     8281 tcgcattgat tcctttgaac gatcacttag attacaaaaa aacgagacaa gtgacgaaag
     8341 atcgtaaagt tggttttcct ggaatggatt ttgtattaat gcatacagga tatccccacg
     8401 gagcaaacac ccctattgga ataaaactcg cacatcctga ttattgtttt ttagcagaca
     8461 gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc
     8521 ggataaaagt agccgattta gaaaagatcg tccatcctat ttacgctgat ttaattagtt
     8581 aagggacgag aagagaagct aagcagagca gagacctcaa acttgagatt atatctgctt
     8641 ggtacttggt gcatcctgta ttatctgtta caattgcttt gtacatactg tactatcaat
     8701 ccaatgaaaa tatatttagc agctatattt tttcattgat gtgcaagtga tgaaatcgtt
     8761 tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg
     8821 acattcctgt ttaacaattg aaatggaaaa tggccaacgc gtgatgattg acccgtttat
     8881 tacaggaaat ccattgacag acttaactgc agaagaagta aagacagatt ggttgctgat
     8941 tactcatggt catagtgacc acataggaga tatgattcct attgcaaaag aaaatcaagc
     9001 cacaattatc agtattgttg agattgctga ttatgcgaac agcagaggag tagattcatt
     9061 cggtatgaat atcggtggaa aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc
     9121 acagcatagt tcaagctatg aagtagacgg tatcgttcag tatatgggcg agcctagcgg
     9181 aattattatt caagcagaag gaaaaacgat ttatcatgca ggggatactg cttatttcag
     9241 tgatctagga ttgcttgcag aagaattcga tattgatgtc gctttcttgc cgattggcga
     9301 taactatacg atggggccag aagatgcagc aaaggctgcg caaacaattc gtgccaagaa
     9361 agtagttcca atccattaca atactttccc tgtcattgaa caagatcccg aatcttttgt
     9421 agagttactg gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta
     9481 ggcagaaaat ggcgacaaaa catgatttga ttttagaata tattgaaagt ttacctgttg
     9541 gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt
     9601 accgtgcaat caaagatgca gaaaatgtcg gactagtttc aacaattcaa cgagtaggga
     9661 ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg
     9721 tcaggatcat tgaaggggac gttttaggcg gggcggcagg attagacaag gtcttgaata
     9781 aattcgtcat cggcgcgatg actaaacatg ccatggaacg ctatattacg ccaggatcac
     9841 taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc attggaagat ggcgccgcag
     9901 tattgatcac aggaggattc aatacgactg ctgagattgc aaagctagct gatgatcttg
     9961 aaatgccgat cttacgcacc tcttatgata catttacagt cgcaaccatg atcaaccgag
    10021 cgttaagcga ccaattgatc aaaaaagata tcatgcttgt cagtgatatc tatatgccag
    10081 ctgaaaagac tcattatttg cagcagacag atacgatttc tgattatcag cgtttgtcag
    10141 aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg atggggattg
    10201 ttacggcaaa agatgtttta gggaaatcgc caaaccagat catcgatcgg gtgatgacga
    10261 aagaaccaat cagtgtaaag aaaacaatga gtattgcctc tgttagccat caaatgatct
    10321 gggatgggct agaagtcatg ccggtcgttt ccgatgactt gacattggaa ggcttgatta
    10381 cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag attgcagata
    10441 cgatttcgga tcagatatct ggaaatatcc atacgattga tactgaccgt gaaggaaatc
    10501 gtttggaaaa ccctaagttc aaattcgtag tagcaccaca aatggtcaat ggcgttggaa
    10561 ccatttcttt tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa acaatggtga
    10621 tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct acattatttg cggctgatcc
    10681 aattggaaag cgaattagac attcgtccac gtgtattgga aatcggcaga cgttccgcta
    10741 aactggatat cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc
    10801 aagtaatgga acgttcttag aaagcaggag tgaaatgaca gtacagcaag aaatagtaga
    10861 tacaataaaa aaatacgata aaatcattat ccacaggcat ttgcgccctg atcctgatgc
    10921 attaggatca caagtaggat tagccgaact attacgggaa agtttcccag aaaaagaaat
    10981 acgtcaagta ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc aagaagttga
    11041 agatgtcttc taccaaggag cgctagtcat cgtgactgat acagctaatt cacccagaat
    11101 cagtgatcaa agatatcagt tggcagaaaa actgatcaaa atcgaccatc atcccaatga
    11161 tgacccttat ggagatcttg tgtgggtaaa tacaaaagca agtagctgta gtgagatgat
    11221 tgcagaattt gctttgatgt ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt
    11281 actttatgca ggaatcgtcg gagatacagg gcgctttttg tatccctcca cgacgtcccg
    11341 tacattagag attgcttcga tattgagaaa ttatccattt gatgcaagtg ctttgaacag
    11401 agagattgaa caaatgccga tgaaagtcgc taaactttcc ggttatcttt atcaaaatat
    11461 ccaggtggat gaaaatggtg caggaagaat aatccttccg caagaaattc ttgataaata
    11521 tggaatcgat gattcagaga cagcttcgat cgtttcactt cctggagtga ttgacgaagt
    11581 gctggcttgg gggatttttg ttcagcagcc ttcagggtat taccgcgtac gtttacgttc
    11641 taaggggccg atcatcaatg aattggctaa acgccatcat ggaggaggcc atccattagc
    11701 aagtggtgcg aatgcaaaag atttagcaga agtagaggaa atctataaag agatccaaga
    11761 tatatgccaa tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt
    11821 ttgtagtaaa aatcacaaat gtatcttttc aagatcttct ttttccgtta caatagtgct
    11881 cactaaagat tttaaaggag aaagaagatg acattaccaa attgcccaga atgtggctca
    11941 gagtacacat atgaagatcg aggcttgttg atttgcccag aatgcgggaa cgaatggaac
    12001 aaagaagaac aagaaactgt catagaagac ggattagtgg tattagatgc aaatggaaac
    12061 cgtctgttag agggcgatag cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca
    12121 ggtgccatta aacaaggaac aaaagtcaag aacatccgtt tagtagaagg cgatcataat
    12181 atcgattgca aggtagaagg atttggaccg atgaaactga aatcggaatt tgtgaaaaag
    12241 aattaaagaa tattcaaggt cttttcacat tcttttccta ctactaatga taaaataaag
    12301 taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt tttagatccg
    12361 acatttcttt tggtcattct tggattagtt atttccggaa ttgcgtcagc gtacgtcaat
    12421 agtactttcc gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg tacacaagca
    12481 gcacaatata tattacaaag tcaacagata aataatgtcg gtgttcaaca aattgccgga
    12541 gatctcacag ataactacaa ttcaggtaat aagatgttga gtttgtctca agcaactgca
    12601 cagtctactt cagttgctgc gataggtgta gcagctcatg aatgcggaca tgcggtacaa
    12661 gatgcttcgg gttatgtccc attgaagatt cgtgcagcac ttgttccagc agctaacttt
    12721 ggttcgatga tttcgttccc cttgattatg gtaggtgtat tgcttagctg gaactctacg
    12781 ttaatcaata tcgggatttt cgctttttct ttagcattac ttttccaatt ggtgacatta
    12841 ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg cggactactg
    12901 acggaagaag aagtaccgat ggcaagacac gtattatttg cagcagcact gacttatgtt
    12961 gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga tcttattgtt cggcggcaac
    13021 cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt gatctgagct aataccgaga
    13081 gttagctgat tgaataccat ttatttggaa taa
//
LOCUS       DAGCXG010000071        13092 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGCXG010000071 DAGCXG010000000
VERSION     DAGCXG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.074"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..384
                     /locus_tag="MB022_001969"
     CDS             <1..384
                     /locus_tag="MB022_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBT4466960.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYHK
                     AAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKE
                     DFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            400..549
                     /locus_tag="MB022_001970"
     CDS             400..549
                     /locus_tag="MB022_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466961.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            630..1226
                     /locus_tag="MB022_001971"
     CDS             630..1226
                     /locus_tag="MB022_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4466962.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1239..2138
                     /locus_tag="MB022_001972"
     CDS             1239..2138
                     /locus_tag="MB022_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4466963.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2141..2626
                     /gene="ssb"
                     /locus_tag="MB022_001973"
     CDS             2141..2626
                     /gene="ssb"
                     /locus_tag="MB022_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4466964.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2992..3252
                     /locus_tag="MB022_001974"
     CDS             2992..3252
                     /locus_tag="MB022_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466965.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3699..3905
                     /locus_tag="MB022_001975"
     CDS             3699..3905
                     /locus_tag="MB022_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBT4466966.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3905..4129
                     /locus_tag="MB022_001976"
     CDS             3905..4129
                     /locus_tag="MB022_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466967.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4144..4581
                     /locus_tag="MB022_001977"
     CDS             4144..4581
                     /locus_tag="MB022_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466968.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            4574..5281
                     /locus_tag="MB022_001978"
     CDS             4574..5281
                     /locus_tag="MB022_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466969.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            5662..5841
                     /locus_tag="MB022_001979"
     CDS             5662..5841
                     /locus_tag="MB022_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466970.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5934..6119
                     /locus_tag="MB022_001980"
     CDS             5934..6119
                     /locus_tag="MB022_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBT4466971.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            6153..6584
                     /locus_tag="MB022_001981"
     CDS             6153..6584
                     /locus_tag="MB022_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4466972.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            7521..8795
                     /locus_tag="MB022_001982"
     CDS             7521..8795
                     /locus_tag="MB022_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4466973.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            8849..10276
                     /locus_tag="MB022_001983"
     CDS             8849..10276
                     /locus_tag="MB022_001983"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBT4466974.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            10289..11161
                     /locus_tag="MB022_001984"
     CDS             10289..11161
                     /locus_tag="MB022_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4466975.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            11256..12251
                     /locus_tag="MB022_001985"
     CDS             11256..12251
                     /locus_tag="MB022_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4466976.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(12856..12963)
                     /locus_tag="MB022_001986"
                     /pseudo
     CDS             complement(12856..12963)
                     /locus_tag="MB022_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4621 a         2012 c         2577 g         3882 t
ORIGIN      
        1 ggacaaatcc aatccactga ttttggatcg acgttacaag aggtgaaagc agccagtgaa
       61 aagcaagaag ccgcgcgtca tttagggatt tgtccaaaat gccaggaaca agaagtttta
      121 ctctatcata aggcggccgc atgtacgtct gaagcttgtg attttagatt atggacaacg
      181 attgccaaaa agaaactcac agcgacacag ctgaaggaaa tcattcaaaa tggccgaaca
      241 agccagccag tgaaaggatt aaaaggtcaa aagggttcgt ttgaagcaac gattgttcta
      301 aaagaagact tcacaacgag ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa
      361 agaacacgcc gcactacaaa ataatggagg ggttatctta tgcgaaatac aacacacaaa
      421 caacaagaaa tcgaaaatca aaaagcaaaa attcgttcag aacaagagaa attacgtgcc
      481 agtaaaagaa gacttcagca attaaagaac cagcaacgga ttcaaaaaga aattgaatta
      541 gatcgataaa atcttcctgg ctattgtata cgcataaact tatgttatac tatatttata
      601 agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtttac aagctgctag
      661 aagaatttaa cggaactttg gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa
      721 cgatcaaacg tatggtagat cgtggagagc tggatcgtga gtatccaggt tttttcacct
      781 tacctggtca atttcctgat gaattgttta tggcacaaaa gaaatatgag cgcggcatta
      841 tttcgcatat caccgcgttg gatttatatg atttaacgga tatgatccct agacagatcg
      901 atctaaccgt accttacggt tatcacgttt ctgaaaaggg gttgaaagaa ttcgcagttg
      961 agttacacta ttcaaaagcg gaatggtatg aattaggtaa gatcgaaata aagagtcgtt
     1021 atgggaatcc tgttatagct tatgatccag aaagaacctt gtgtgacatt tggaatccct
     1081 ggtacaacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg gagtcggaca
     1141 gaaaaaatct tagaaaattg aatgagtatc gacgaatttt gcctacagac aaaacgatac
     1201 gctcttatat tatggcttta aattaaagga gaagacacat gacacctacg caattaaaag
     1261 caaaagtgaa gaatatttcc agagaaaaag aagttgatcc gcagcttgtt ttaagacatt
     1321 ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa aattttgtgt
     1381 taaaaggcgg cttcttaatt ggctcaaagt atggcattga aaatcgaaca acaaaagaca
     1441 ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attgacaacg gttctaaacg
     1501 atattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga atgaaagaaa
     1561 caagggaagc tgattattat ccagggttta gcttacgtgt actggcacat ttggaaaaca
     1621 tgcggccaga ttttaaggta gacgtgacga caggtgactc gatctatcct gcaacgatca
     1681 ctcacagtca taaattgatg tttgaaaatc gaacgattcc tttggaatct tatcctacag
     1741 aacaaattat tgccgaaaaa ttaagtgcaa cctttgattt tttaacagat aattcgagag
     1801 gaaaagattt ttatgatctc tatactattc ctaaaatgga aaagatcgat gagaagacct
     1861 tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg ttaaagattt
     1921 attacgaaaa ggatatggat gtactgaaaa atagtcatga gctaaaagat acctggaaga
     1981 aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgaagagaca atggattcga
     2041 ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa atagcttatc
     2101 aaagacacca acagatgaac agagaaatgg agagataaaa atgatcaata atgtaacttt
     2161 agtaggacgg ttaaccaaag atccagattt acgttatacc gcaagtggca cagcagtggc
     2221 tacttttacc ctagccgtta atcgtaattt cacgaatcaa aacggcaatc gagaagctga
     2281 ttttatcaat tgcgtgattt ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa
     2341 aggcatcttg atcggcgttg tcggacggat tcaaacacgg acttatgaca accagcaagg
     2401 acaacgtgtg tatgtgacag aagttgtagc tgataacttc caactattag aaagcaaagc
     2461 agcgacagag agccgtgcgc acgctgatca atcgtccacg agtccaagca ccaccacttt
     2521 tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac agaatccatt
     2581 tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttaagtag cattttctat
     2641 tggttgcatt tgctacggtt tatgttataa tttagtcaac aaaaagaatt ttcaatctat
     2701 aaccaaaaac cactagctgg cgggctagtg gttttctttt acgtttctat tataagaatt
     2761 aacgagtagg cttactgtct ttcttggcaa atttacaatc aagccatttg cagatatagt
     2821 gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc aaacctataa
     2881 cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta tagcataatt
     2941 gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt gatgaacaaa
     3001 tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt ggttcgcttt
     3061 actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga aattatcgat
     3121 aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca cttcaataaa
     3181 cgaaatcagc tggttattgc tagcatgtat gttagaaatc ccgatcattt tacacgatcg
     3241 atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc tctttttgtt
     3301 ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc cttctcaatt
     3361 aaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgct
     3421 gcacgttcac taagatatgg tgtcttgcaa agcaagacaa aataggaaaa gcttcctctc
     3481 accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg gcaacaagtt
     3541 gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg cgcccttgtc
     3601 cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa
     3661 ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt
     3721 ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact gtcgaatgcg
     3781 attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt ccaacatgcg
     3841 ggaaaaaata taccttgtct tatggcgatt acattttgat tgatgagaaa ggaaagaaac
     3901 gctgatgaaa acacaacaca aattattgaa tgtaaaagaa gtcatttctg aaaaagtagc
     3961 agaagcttta cttattccgc ggtactggac gatagaaacc cctttagtag aggacgcacc
     4021 gactatgcta tttgaagacc tagtgaagat tgcaggatat gaactgcatt atcaagaaaa
     4081 cttggaaact tatgaaaacg aatattggtt aaccaaatta gagaactaaa aaggagaaaa
     4141 aatatggctc agaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
     4201 aaaatggagg atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag
     4261 gaaaaattgt cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt
     4321 gcgacgtact atcgaacatt ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa
     4381 gccttactat gcttggaagc aggcgtcaaa ccaagcacaa tcaaaccttc tgaataccaa
     4441 gctttagaac taacgtatgg aaaatttatc gaaacgaaaa aagccaagac tgtttcaagt
     4501 gaatggttgg actactttga aaatgtcaac aagtatggcg catgttacat catgaaaaag
     4561 gaggacaacg aaaatgagta atgtggttta tcaactaaca gtggaagatg tttctcactt
     4621 tttaacaaac aaagaacatt tgaatgaagt agtcagacgg attcaaaaag actttagttg
     4681 tatggaagaa atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagcga
     4741 aacgagtcaa acgaaggtca cagtcagcca taatcattta gacgtgtata aacgtcgtcc
     4801 tgaaacggta aaaaaaggaa cagagcttct acgactagaa acagaaaaat acatcatgaa
     4861 tgtgtaccta gaaaggagtg agctggtcga aaactttgtc agctacacga tttgtaggaa
     4921 acactattac tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga
     4981 agaaatggaa gagctaaaca gcaaaaatgc gacggtagac gattattacc ttgtatttac
     5041 tagatttctg acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc
     5101 tgtacccgaa gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg
     5161 gtacacgact ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca
     5221 aaatgagctt gactgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata
     5281 accatttata ccgaaaagtg agaatgtgtg aaataagaac aaaaaacctt gaaattaagg
     5341 gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgctcagg tggtgcgaaa
     5401 taagcgggca acgaccgata acctaagaga aagaaggaaa tccatatgaa taaaatcatt
     5461 cctctgttgt tcgtactgtt tgttggcgag atacttatgc gtcatgctgc agctgtacat
     5521 ttaaacttcg tagtattctt aggaatactg gtattttgtt tgatcttttg gcgatttgta
     5581 ttagcccttg tgagtctact attcaagatg attgcagcca tatttttcat agggttattt
     5641 cgatgaaagg agagagtact aatgccaatt gcaataggaa ataaacgttt acccgtgacg
     5701 ttggatgaaa agagacaaaa agaattgcag gaactcaaac agaagtacgg caaaagtgaa
     5761 tccaggatta tgtgtattgc gttagattta ttgattgcac aagaaaaagc agggtttgat
     5821 gtaccagcac tcaaaaagtg acgtcacttt tcatttgaag aaacaaaagt gatagcactt
     5881 tacgcacgtg tcgatacgtg ttatgattat tttgtaagga ggtatagtgc acaatgccac
     5941 ttacgggaaa agaaatgctt aagttattga aaaaaaatgg ctgggttgag cgaaggcaag
     6001 agggatctca tcatcatctt tataaggatg gtgttagaat cactgttcct gttcatgcca
     6061 atcaagatct tggccgagga cttgagcaaa aaattttaaa ggatgcggga ttgaaataat
     6121 ctcgcattcg attaaagaaa ggattgactg aaatgttaag tgagccttta gcaaaaacct
     6181 atcctgctat ttttagccca gaagaaggtg gtgggtactt tattgaattt cctgatgttc
     6241 aaggtgctta cacaggaatt aacgaagacg atatatctta tggaattgct atggcacaag
     6301 aggtgttagg tatggtttta gcagattata ttgagcatga agatctactt ccagaaccaa
     6361 caccaatcaa taagatttct gttgaagatg attcttttac tactttaatt cgtgtagatg
     6421 ttgccaaata tttgaaagac acagaacttg tcaaaaaaac cttaacaatt cctcaatggg
     6481 cggataagct aggcaaaagg gctggcataa atttttcagt ccttctaaca gaatcaattg
     6541 cagataaagc agataacatt cttcgttctg gaagaaataa ttagaactca aaagagtcag
     6601 aaacttaaaa atctttggct tcttttttta ctacaaagat ggcttcttta aattatttgc
     6661 ctgattgatt taaaaaaatt gcgcaaagac tacgagctaa ttaagttgct cgtggtcttt
     6721 ctttatgaaa actttttaga tctagataaa aacattgaga cactttgaaa aattttttta
     6781 atagtaatgg gcgcttctat gtcaagctga ttcttctttc ggtaaattaa ttttacttag
     6841 aatatcacta tcttgataaa aaatggtgta ttcacaaatt ctggcataaa aaatacctac
     6901 tttctagtta aggttcctga ataagtcgca atccaattat ttccagctga tttataactt
     6961 tttaaatata attttccata ataccaataa ccattacttt tttctgtcca atttcgttat
     7021 tgagaagatc tagaaggcca gccaacttta tcaaaaatca tcattcctgt cactcgtatc
     7081 gttttagaag ctgctcgagt aagtaatgga gaaactgtta aataatttta agtaacagtt
     7141 aagtcgttag ttgggttatc aatggctatt ccgtccttat ctgaacctcg aaacccaatc
     7201 atagaaaaag ttttttgaac aagttttaac agataaatca agttatatac tagaaaattc
     7261 gtaaaatata tttttaatgt cataatcgtt tgatttttct ggattgacat ttatttataa
     7321 aaaatagtgc cattaaaaaa gaaaagtgac acaccaataa aagtcatatc ctgtgacatt
     7381 atatggcatt atccaaatag aataaccggt tgacatgtaa gcggattcct tttataattt
     7441 taggagtata agtccttttc taaaaatatt aaccggttat catgaaaggg gatacaaata
     7501 tatcgttgga ggataaaaat atgtcaaagg ttaacgtatt taaaaatcaa tcctatttac
     7561 aaagttcagc tacattatta ctattttttg cttcttgggg tgtttggtgg tcattttttc
     7621 aactttggct aacatctgaa tcaaatggtt tagggttatc tggcagtgct gtaggaacag
     7681 tattctcggc aaattcgtta gttaccttaa ttttgatgtt tatttatgga acattacaag
     7741 ataaattgta tattaaacga aatttattaa tttttgcttc tgtattagcg acacttgttg
     7801 gaccattttt tatatggata tacgggccat tgctagataa caattttaat ttaggcatta
     7861 ttatgggagc actatttttg tcagctggat atttagcttc tgtaggagtt tttgaagctg
     7921 tgtcagaaag gtttagtcgt ttatttggct ttgaatatgg acaagcaagg gcgtggggat
     7981 catttggtta tgccttggta gcgcttttgg caggattttt atttgtaaaa aatcctcatt
     8041 taaacttttg ggcgggatct ttctttggtt ctttactatt gttaaattta ttattttgga
     8101 accctaaagt tgaacgggaa gcaaatcaaa attttaatca agaacaagct gaatcaaata
     8161 gtattccttc tttaaaagaa atgtttgatc taatgaaact gcctcaatta tggacgataa
     8221 tcatctttat tgtttttaca tggacatttt atacggtatt cgatcaacaa atgtttccgg
     8281 gattttatac tggtttgttt tcaacatcag ctaatggtga aaaaatatat gggacattga
     8341 atgctattca agtattttgt gaagcgttaa tgatgggaat tgttccaatc attatgagaa
     8401 aattaggggt tcgaaatact ttgttattag gtgtaaccat tatgtgtgta cgaattggat
     8461 tgtgcgggtt tgcctcgaca ccattatctg tttcatgcat aaaaatgttg catgctttag
     8521 aagtaccatt atttacatta ccaatgtttc gctattttac acttcatttt gatacaaagc
     8581 tatcagcaac cctctatatg ataggatttc agatagctgc tcaaattggg caagtgattt
     8641 tatcaacacc attgggaata ttaagagaca acgttggcta tcaaccaaca tttaaaatta
     8701 tttctcttat tgtattacta gcaggcatat atgcattctt tattcttaaa caagatgata
     8761 gagatgttca aggggatcca tttattcgag gataatggaa accaacagaa taaaaaacaa
     8821 ttttatctag aaagtgagcg aatatattat gacaaaaaga gctatagaat taacaaatac
     8881 acgttatcgt ttgggatatc atgtgtctgc accatcagga tggattaatg atccaaatgg
     8941 attttgctat tttgatgggt attatcatgt gttttatcaa catcatccat atagtgctga
     9001 atggggacca atgcactggg cacatgctag aagtaaggat ttagttcatt gggaatcttt
     9061 accacttgct ttgactcctg gagatcaaga agatgaaggt ggttgttttt caggaagcgc
     9121 tattgaaaaa aatggtgtgt tgtatctgtt ctatacagga catcactatt atggagacaa
     9181 tgatccagat catttttggc aaaatcaaaa catggcgtat agtacggatg gcattcactt
     9241 tactaaatat gaaaaaaatc ctgtaatagc aaaagcacca gaagataaca cacatcattt
     9301 cagagatcca aaagtatgga aacacgatga aaaatattac atggttttgg gtagtcaaga
     9361 aaaagatgga ttaggtcgtg ctattgttta tcagtcgtta gatttattaa attgggatta
     9421 cattggacca atttctaaag cgaaggaagt acttacagaa ggatttatgt gggaatgccc
     9481 agactttttt gaattaaatg gaaaatctat tcttttatta tcccctcagg gaattgaagc
     9541 aaatggaaaa gattatttaa atctatatga aacaggctac tttatcggtg attatgatta
     9601 tgagacagca aaattcatta gaggaaattt ttatgaatta gataagggac acgatttcta
     9661 tgcaacacaa acaactttat ctccagatgg tagacgtatt gttatggctt ggatggatat
     9721 gtgggaaagc cctatgcctg aaaaagtaga cggatgggca ggagcattaa cgcttcctcg
     9781 agaattagag ttaattggag atcatttgta tatgaaacct gtgagagaat tagaacaact
     9841 tcgagttggc agcggcgttg aaacaaaagt taatttggca ggagaaataa taatcgctca
     9901 agatgcttca ttcaaagaag ctctaattga tgtactattg actaaatcaa aaaatcaaga
     9961 aatcaaattt tcattaaaaa caaaagatga agagttaatt aatatttttt attctaagga
    10021 taaaaatgaa tgtaccttaa ctcgtaacgg caaaaatgat ccaaggtatg gaaccattca
    10081 gatgtgtgat agattatcac ttcgagtgtt tatcgataaa agttcggcag agatttttat
    10141 taatgatggc gagcttgttt ttacagagcg ttattataca gaaaatcaac caagtatgca
    10201 tttgagtgtt agtgaagaag cttttgtctc gagtacaatt tatcatctaa aaaaagatgc
    10261 aatttcttac gagtaaggag gttttatgat gttaattggt agcatagaag caggtgggac
    10321 taaatttgta tgtgccgtgg gagacgagga ttaccgagta caagaccaaa taatttgtcc
    10381 aacgaccacc cccagggaaa cattgaaaaa aactgttgac tattttaaac agtttgatat
    10441 aaatgcatta ggtattgcat catttggtcc aatagaaatt cgaaaaaatt caccaaaatc
    10501 aggatatatt acgtctacac caaaggtggg atggcgaaat gttaatgtgt taggttattt
    10561 acaagatgag ctgaatgttc caatgtcttg gacaacggat gtgaacggat cagcttatgg
    10621 agagtacatc atgtccactt tatcgaatga aaaaataaat tcattagttt attacaccgt
    10681 tggtactggc gttggtggtg gagctgttgt tgacggaaaa ctattaggat cgtttggtca
    10741 ttcagaaatg ggccatacgt ttgtaaaacg ccatcctgat gatattgatt ttaaaggaat
    10801 ttgtccttat catggtgatt gtttagaagg cttagttgcg ggaccaacgt ttgaagcacg
    10861 ttttggaaaa aagggtgaag acgtttcttt aacggatcct atttgggatg taatggccta
    10921 ttatctagca caagctgtga ttcaaacaac attgattata cgaccggata aaatagtatt
    10981 tggtggaagc gtagtaaatg aaaaattgtt agaaaaaatt cgtgttaatg ttaatgaatt
    11041 attggatgat tacgtggaaa taccaccgtt aagtaaatat attacgcgtc ctttaattaa
    11101 aaataatgga tcggcaacac taggaaactt tgctttgggc ttgaatcaat tacaagaata
    11161 aaacatagaa aaaatatcga ttatcaggta tagtagttct atagagttct ttttttcgat
    11221 agttaattat tgcattattt aggagagaca agagaatgcg cccaaaatta gaagatgttg
    11281 ccaaaagagc aaatgtatca aaaacaactg tttcacgtgt attaaacaat cgtggctatt
    11341 taagtcaaaa aacgattgat aatgtttata aggctataga agaactcaat tatcaaccga
    11401 acgttgtagc gcgtcaatta tttcaaaaga aaactaacat tgtcgggtta ctatttccta
    11461 cagtagccaa tccttttttt agtgaactcg ttgaggcatt ggaaaaaaag ttatatgaaa
    11521 ttggttataa ggtattaata ggcaattcga tgaataataa agaaaaggaa acaaattatt
    11581 taaatcagtt attgtctgat caagtagatg gtttaattgt tggtacacat aaccaaggga
    11641 ttcaggaata taaatatcag aatttaccga ttgttgcaat tgatcgagtg atgaatgaag
    11701 atattccagt tgttgaatcc gataactaca atggtggaaa acttgcaacc aagttattaa
    11761 ttgcacaagg tgcaaaaaat attattcata caaacggtcc aattgactta cagactccgg
    11821 ctaatcgtcg gagactagcc tatgaagata ctatgaaagc atatcagtta attccacgaa
    11881 ctgtgacatt agatttcaat atttcttatg taaagaaaaa acagattttt ttccaaatgt
    11941 ttgaggatta tcctaaaata gatggtattt ttgcttcaaa tgacatcgat gcggccctaa
    12001 tattacaagt agcaaaagaa aaggggttaa atgtaccagc tgatttatta gtagtcggat
    12061 atgatggtac tttaatgact cggagtatat taccagatct gacaacagta atacaaccaa
    12121 ttaatgatat agcagacaca gctgttgcta ttttgatgaa acgaataaat aaagaagaaa
    12181 caaaaaagga atatatttta cctgtcacat tatggatggg gaagacatca gtaaagaaaa
    12241 aggacaatta aatttataaa gactatcaat gataatttca ttattaattc gtgatataaa
    12301 aagttgttaa atctcttata atagactatg atacaaaatc atgcttttaa tctgtgtaga
    12361 tgttgccaaa tatttgaaag acatagaact tgtcagaaaa accttaacaa ttcctcaatg
    12421 ggcggataag ctaggcaaaa gggctggcat aaacttttca gtccttctaa cagaatcaat
    12481 tgcagataaa gcagataaca ttcttctttc tggaagaaat aattagaact caaaagggtc
    12541 agaaacttaa aagtttttga tcctttattt tcaagtctga tttgacagat tttcggcagc
    12601 tcatttattt atggtcagaa atagttgctg aaattttaat ctattagcaa atagtccaga
    12661 acgtatgaag aggattcgga tatcatttgt cattaacacc ataaatattt ataaaattcg
    12721 tgttcaccta caattaaatt taatatataa tattcgttgt aagggcttat aattttttgt
    12781 taatgaacaa gatatgtaat tgatagtgtc aatagctatt caaaaatgta ggcttttggc
    12841 ccataaataa caaggttact tatatgataa ctttttttct ttataatctt taatccgata
    12901 gagctttcca gtagtttgaa taatcttagc gtggtgcact aatcgattga ttagtgcatt
    12961 tgtgttcagt ccattgagac ataatagtgg atagttttac tgagaaaatt gataaaaaat
    13021 agagatataa ttcaaatacg tatataatga aactaaagat aaatacggag gaaaatatat
    13081 gattataaaa aa
//
LOCUS       DAGCXG010000072        12993 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGCXG010000072 DAGCXG010000000
VERSION     DAGCXG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.075"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            375..932
                     /locus_tag="MB022_001987"
                     /pseudo
     CDS             375..932
                     /locus_tag="MB022_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            990..3029
                     /locus_tag="MB022_001988"
     CDS             990..3029
                     /locus_tag="MB022_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBT4466977.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAVIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRAPELLQYLNQNYQ"
     gene            complement(3138..4289)
                     /locus_tag="MB022_001989"
     CDS             complement(3138..4289)
                     /locus_tag="MB022_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBT4466978.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4443..4973)
                     /locus_tag="MB022_001990"
     CDS             complement(4443..4973)
                     /locus_tag="MB022_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4466979.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5072..5422)
                     /locus_tag="MB022_001991"
     CDS             complement(5072..5422)
                     /locus_tag="MB022_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBT4466980.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5579..7108)
                     /gene="ahpF"
                     /locus_tag="MB022_001992"
     CDS             complement(5579..7108)
                     /gene="ahpF"
                     /locus_tag="MB022_001992"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBT4466981.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7118..7681)
                     /gene="ahpC"
                     /locus_tag="MB022_001993"
     CDS             complement(7118..7681)
                     /gene="ahpC"
                     /locus_tag="MB022_001993"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBT4466982.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7825..8487)
                     /locus_tag="MB022_001994"
     CDS             complement(7825..8487)
                     /locus_tag="MB022_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4466983.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8671..10317
                     /locus_tag="MB022_001995"
     CDS             8671..10317
                     /locus_tag="MB022_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBT4466984.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10308..11390
                     /gene="uxuA"
                     /locus_tag="MB022_001996"
     CDS             10308..11390
                     /gene="uxuA"
                     /locus_tag="MB022_001996"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4466985.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11498..12993)
                     /locus_tag="MB022_001997"
                     /pseudo
     CDS             complement(11498..12993)
                     /locus_tag="MB022_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4351 a         2382 c         2421 g         3839 t
ORIGIN      
        1 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
       61 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      121 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      181 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      241 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      301 caaactcgct cctcacctaa caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      361 gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac
      421 aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa
      481 tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa
      541 aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg
      601 attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt
      661 tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt
      721 atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag
      781 tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag
      841 cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt
      901 gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa
      961 ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc
     1021 gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt
     1081 tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc
     1141 aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa
     1201 aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agataaatac cagaatatat
     1261 acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg
     1321 attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag
     1381 atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac
     1441 ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag
     1501 cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa
     1561 aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca
     1621 aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa
     1681 gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac
     1741 ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag
     1801 ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat aagaatccag
     1861 aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc
     1921 ttcgtgggac aacaggtggg aaattaagca ttacagatgc agacggtgta gagaaaaaag
     1981 ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg
     2041 cacaaaaaat agcttttgac agtctaggag gaaaagctgc atcaactgtt gcgacaacgc
     2101 caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga
     2161 caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca
     2221 tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaatgatt acagcagtaa
     2281 tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat
     2341 ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt gatgtatcac
     2401 aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt
     2461 tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc
     2521 ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag
     2581 atatcttgct agctgatact ggatatggac agggcgaact tctaattact cctatccagc
     2641 aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct aaattgattg
     2701 cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg
     2761 tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt
     2821 tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa
     2881 aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg
     2941 tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca ccggaactat
     3001 tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact
     3061 gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca
     3121 acttcttttt gatttactca cacttcataa actttttttg taaaatattt gagaacaagt
     3181 ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg
     3241 atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc
     3301 ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa
     3361 taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt
     3421 tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata
     3481 aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga
     3541 ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct
     3601 acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac
     3661 actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa
     3721 acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc
     3781 cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca
     3841 gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact
     3901 tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt
     3961 cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca
     4021 agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc
     4081 agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg gactagaata
     4141 tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca
     4201 atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat
     4261 atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa aatatagccc
     4321 gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac
     4381 aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat
     4441 gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg
     4501 aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac
     4561 aataagacga atccgccaat caacaacaga atactactat aaacactcca acttttagct
     4621 tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga
     4681 agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc
     4741 aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg
     4801 taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg
     4861 ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca
     4921 gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa
     4981 aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa
     5041 tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt
     5101 tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat
     5161 cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat
     5221 tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt
     5281 ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta
     5341 tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg
     5401 taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact
     5461 tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa aaggattgtg
     5521 acaaaattct tgtcaccatc cttttttccg ctctctgact ttttacaatc ttcatagctc
     5581 aagtgcgcat cagataatcg aatgctccta aagctgcagt agctcctgaa cccattgaaa
     5641 caatgatttg tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg ccggggatat
     5701 tcgttgcgcc atttttctcg gtcactattt catttcttgg attcatttcg atcgttctat
     5761 ctagccaatc cgtattaggg agtaagccga ctagaatgaa tactcctgaa acagctagtg
     5821 tatgcatctc catcgtacgg cgatcgatat aggagagaga ttctacctga ttcgtaccgc
     5881 tgatttcttt cgtcgctgca tgtttgataa cagtgacatt cgcaagagag actagtttat
     5941 cctgtaagat ttgatctgct tttaattctg gtaagaattc tagaacatat acatgttttg
     6001 ctaacccagc caaatcgatt gctgcttcaa caccagaatt accaccaccg ataacagcaa
     6061 tctcttttcc tttaaataaa gggccgtcac agtgtgggca ataagcaatt cctttatttt
     6121 taaactcttt ttcgcctggt acattgatag aacgccagtg cgcacctgtc gcgatgatgg
     6181 cggtttttgc tttaagtgat ataccgttag ctaagccaat attaataaaa tgttgctctt
     6241 tatttaagga aacggcctgt tgattcatca taatgtcgat agggtaacta cgcacatgac
     6301 tttctacttg ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc atattttcaa
     6361 tccctagtgt ttcaacgact tgtccgccga aagaatcagc gacaagtcct gtacggattc
     6421 ctttacgtgc tgcatagata gctgcactac tagctgctgg tccaccgcca atcactaata
     6481 catcataagg ttctttttca aacaactctt cttcaatcaa agggccagtg attttttcaa
     6541 caatttgttc gatcgtcatg cgtccgctac taaattcttc tccatttaaa aacacagttg
     6601 gcactgccat gattttttta tcatcgattt ctttttggaa catcccacct tcaatcatcg
     6661 tatgagtaat atttggattt aatacagcta atatattcaa tgcctgtaca acgtcggggc
     6721 aattatgaca agtcaagcta acataactgt caaaatgcaa tgtttgtgtt atttttttga
     6781 ttttaagact caaagattca ctgattttcg gtgcccgtcc actaatttgt aacatagcca
     6841 gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc aaaaacgatt ccactttctt
     6901 tgccaactgg attgatagaa aaactaggtg tgcgcatcga atctcttttt tcccaagaga
     6961 ttttaggaga catttctgct atttccttcg taaattcagc tagtttttga ctattttcac
     7021 tattatccaa actaacagaa aaaacaatag gagattcaat cagtgctaaa tattgttcga
     7081 gattttgttt agtttctttg tctaacatat ccaccaccta aatctttcca acaagatcaa
     7141 aacttggttt caatgtttcg ccactttctt tccattttgc cggacaaact tcacctgggt
     7201 gggtacgtac atattgagca gcgcgaatct tatcaatcag tgcactagcg tcacgaccga
     7261 ttccatctgc attgatttcc atagcttgaa cgattccgtc tggatcaata ataaatgttc
     7321 ctcgctgagc taatccttgc tcttcatcca aaacatcaaa aatacgagaa atctgatgag
     7381 acggatcacc gatcatcacg tattcgattt taccgatagc atctgatgta tcatgccatg
     7441 ctttgtgagt aaagtgggta tcggtagagc aagaaaagac ttcaacacct aaagatttca
     7501 atgtactgta ttgttcttgt aagtcttcta attcagtagg acaaacaaaa gtaaaatcag
     7561 ctggatagaa gcaaagaata ctccagttcc ccataagatc tttttcactg acttctaaaa
     7621 attctccttg atgataagca tgtgcattaa attcttcgat ttttttccca atcaatgaca
     7681 tagtaaaacc tccaataata attatttatt tataattatt gtaatttagt tggagttgca
     7741 aagtcaatgg atattttgtt atttaataaa gattactcaa aaacgaaggt taattataaa
     7801 aatagaagag gtcgcaagaa aatgctactg ctggaaatac tcgggataac gttgagtaac
     7861 agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc
     7921 tggtttttta tcaactaaag ccttgagtaa atcataatgt tgttccatga tcgtttccca
     7981 tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg tattatggtc
     8041 atctaaccag ttccaaactt cttttcttcc tgcaatttcg aaacaagctt catgaaataa
     8101 attatctgca cggaaaaaag cgcgttcgtc atttattttg actgcttttt cttgttcttc
     8161 taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat
     8221 gatttgctct tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa caagattaat
     8281 tttggaaaca tacgtaccac tttgaggtat cgtatacacg agttcttgct gacgtagttg
     8341 aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca gtgccttttc
     8401 agatattttc cttccaggtt ctaagtcagc atagataatt tgtttacgaa tcgcttgata
     8461 agcttgtgat tgcaagctgc ttttcatctt tattcctcca aaatcatttc tatgaacgta
     8521 gtataacaga agaggtctga aaaacaatct ggtatgtcac attaactagt aaatttgtat
     8581 aaatagtaaa attttggttg acatagcgat ttttattgca taaaattcac ttgaatacaa
     8641 aaactaatat gttagtttag gaggggaaaa atgattgatt taaacaactg gaggaacttt
     8701 atccctgaat tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc atcattaaga
     8761 gaaaatggac aaaaaaatcc tgtctggatc catttcggtg gcggaaatct ttatcgagga
     8821 tttcatgcag aaatcgctca acgtttagcg gatctaggcg aattgtccac tggtgtcatt
     8881 gtttgtgaaa cattcgatga agaaataatt gaaagggctt accaagattt tgatgatttg
     8941 attcttgaag tggtcatgcc aagcagtgga gagttcgtca aacgtatatt agctgcgaca
     9001 gctgacagtc tgtattgtca tccagattgt tctgaacact ttgataagat gattcgacac
     9061 tttgaaaatc ctagtttaca attagttact tttgctatta ctgaaaaagg ttatcgattg
     9121 aaagatacaa ataataaata tataggaccg attgcagtcg atataaaaaa tggtccaaag
     9181 aagccatcac atacaatgag tattcttgct gtgctacttt ataaacgttt tttatctgga
     9241 gcttggccga ttgcacttgt ctctacagat aatttttcac aaaacggtag tcaattacgt
     9301 acttctttgc taacgatcgc aaaagaatgg caaaaggaag gttatgtaca aaatacattt
     9361 atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat tgatcgtatc
     9421 accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt ttcaaatatg
     9481 tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac agaggacatc
     9541 cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga aaaagctggg
     9601 gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt gacgacttgc
     9661 cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta taggagcata
     9721 gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa
     9781 ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct ggaagaagta
     9841 attactgtgc gcttttctaa tccaaatatt cctaatatgc ctcagcgaat cgccacggat
     9901 acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat ggaaatagaa
     9961 gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg gcttcgatac
    10021 cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc actattgaca
    10081 gaccttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc acatattcat
    10141 gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct ttaccaagca
    10201 gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc aaatgccgta
    10261 cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg gaaatgactt
    10321 ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga cagattcctg
    10381 cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta tggcctaggg
    10441 agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta aaagtaattg
    10501 aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag cagtatatcg
    10561 aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc atctgctata
    10621 attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta gaagacggct
    10681 catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa atcgtcgcgc
    10741 gcatcgaaca ttccagcaat gaatttggat tgccgggatg ggaacctgag cgcttagctc
    10801 ctatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt cgagaaaatt
    10861 ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc aaaatggctg
    10921 tacatccaga tgatccgcca tactcgattt ttggattagc tcgaatcgta aagaatcgag
    10981 aagatttaga ttggttgtgt cgcatagtag acagccctta taacggaatc actctttgta
    11041 cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca gaattttgcc
    11101 aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct aatcgagatt
    11161 tctatgaggc accacataaa tccgaatacg gttcattaga catgtaccgc atactaaaaa
    11221 caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga atgatctgga
    11281 atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct gtttatctaa
    11341 atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac
    11401 tgtaatttta ttatgtaaat aagaaggatg ttcaaattct tgatacataa atccggaggt
    11461 tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact tgattttctt
    11521 ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct gagatcctca
    11581 taatcacttt tttccagtct tctggtgtga tgattccttt taaaatatag atttttttga
    11641 tatgagaagg caattgtttc aaagggcagt cgctgataac gatgttgcag ttctccataa
    11701 aatcattttg tattttgatc gattcatttg aaaaaatttg ttctatctta acgatcagat
    11761 ctttactgat ataaaaatca cgcgtatagt tcacaccaat cacaattcgc ggctgttttt
    11821 tgtagcgatc atacaaatgt actaggtctt caaccatcca ttgcacattt tccttttcta
    11881 aaataactgg taacaagtgt tgattttgtt ctctgaaatc catataaaat tgttcaatgg
    11941 cggtttggac ttctttcaat acgctatgtt cttgataaag catgaaagta ggaaggggcg
    12001 tatttggttg attgaagtta agaaatccat tataaagttt atttcgtgcg aatgccaatg
    12061 agtaaagaat cttttctttt tcaggtataa acaaatcaaa tacagtacaa agggactcca
    12121 ccatttctct tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct
    12181 ccaagcttgt ttcttccgat tcgattttcg tgatagaaag tcgagctaaa atattcacta
    12241 agatgcgttg ttcgcgacaa attggcagat taggtcgcaa aatatctaca gagagatcaa
    12301 taaaaaagtc aatatattca tactctgtga attctttttg tacgtctttc acagaagttg
    12361 caggaaaatt ctctttacat atttttaata gaaaatataa tttatctatc ataccgctat
    12421 ttagtcggtc aagtacttct tttttaaaaa aagtattcat cataaaagcc actacttgcg
    12481 gatcttcatc ataaaattct gtagcagtac ttgaaaagat gttccaataa atatctaatg
    12541 ctgcatattg aatttgtacc tcttttccag aaaaagactt tctgccatta ttagaaaagg
    12601 agatagacaa accgtactat gctaaaaatg cattaatctt tttcattttc gtatacaaat
    12661 aagaggcact atagtttgtt tgctgactga aactgataat cgaatttgtc tgacagctga
    12721 acaaagaagt caagatctga taactgactg attcgttgca gtatcttcca aatagacgat
    12781 aatagcagta agccggatca attccttcaa aattcaactt taaaaaaggt ttttgcttat
    12841 caatatgcag ttctgttaga ttgagacgct caaagtcttg ttctaattct ttgatgtata
    12901 gtaaaaaatt tgattcagac atatggaaat gatctgctaa atattgtgta gtaataaaga
    12961 aacgatgttc agataggaaa agaaaaatat cta
//
LOCUS       DAGCXG010000073        12690 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGCXG010000073 DAGCXG010000000
VERSION     DAGCXG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.076"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            192..761
                     /locus_tag="MB022_001998"
     CDS             192..761
                     /locus_tag="MB022_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBT4466986.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            748..1608
                     /locus_tag="MB022_001999"
     CDS             748..1608
                     /locus_tag="MB022_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4466987.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1798..2502
                     /locus_tag="MB022_002000"
     CDS             1798..2502
                     /locus_tag="MB022_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4466988.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2507..4342
                     /gene="walK"
                     /locus_tag="MB022_002001"
     CDS             2507..4342
                     /gene="walK"
                     /locus_tag="MB022_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBT4466989.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4339..5655
                     /locus_tag="MB022_002002"
     CDS             4339..5655
                     /locus_tag="MB022_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4466990.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5656..6525
                     /locus_tag="MB022_002003"
     CDS             5656..6525
                     /locus_tag="MB022_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBT4466991.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            6580..7389
                     /locus_tag="MB022_002004"
     CDS             6580..7389
                     /locus_tag="MB022_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4466992.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7492..8121)
                     /locus_tag="MB022_002005"
     CDS             complement(7492..8121)
                     /locus_tag="MB022_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBT4466993.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8291..9583
                     /locus_tag="MB022_002006"
     CDS             8291..9583
                     /locus_tag="MB022_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4466994.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9659..10321)
                     /locus_tag="MB022_002007"
     CDS             complement(9659..10321)
                     /locus_tag="MB022_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4466995.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10546..10830
                     /gene="groES"
                     /locus_tag="MB022_002008"
     CDS             10546..10830
                     /gene="groES"
                     /locus_tag="MB022_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBT4466996.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10881..12506
                     /gene="groL"
                     /locus_tag="MB022_002009"
     CDS             10881..12506
                     /gene="groL"
                     /locus_tag="MB022_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBT4466997.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4215 a         2180 c         2759 g         3536 t
ORIGIN      
        1 tttttactcc tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa
       61 gaaggcttag ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt
      121 aaagcaacta gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag
      181 gagcggttat gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa
      241 cattccgtct gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca
      301 gaacgttagt tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta
      361 cctcttctat ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa
      421 gatataacaa agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga
      481 ctattcctga aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag
      541 cctataatct attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac
      601 aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa
      661 gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa
      721 ccgaatattt aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat
      781 tcaggtacgg atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg
      841 aaaattattt ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc
      901 tatcaacgtt tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca
      961 gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc
     1021 agtggattga gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg
     1081 gaagcatatg tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa
     1141 gcagcaaaat ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag
     1201 ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt
     1261 ggacggatcg gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc
     1321 tcatgtaatg aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt
     1381 ttggataaaa cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta
     1441 gctgttgccc atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga
     1501 gagttcccac aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac
     1561 actggtccgg gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg
     1621 aaacactgag gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata
     1681 aagtattttg cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa
     1741 agcagaaata cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg
     1801 aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta
     1861 gccaaagaag gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt
     1921 gctgaggtag aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta
     1981 gaagttgctc gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa
     2041 gattctgaaa ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag
     2101 ccattttcca accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg
     2161 gctgcgaagg aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc
     2221 catccagaag cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa
     2281 ttcgaactac tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta
     2341 ttacaaacgg tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta
     2401 cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt
     2461 cgtggtgtcg gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt
     2521 ccgctttttt cgatcagtca attttaagat cgctattacc tttattctga ttttattgat
     2581 ctcgatcgaa attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac
     2641 atttattaaa gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt
     2701 gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga
     2761 aaacagtgcg actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg
     2821 aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat
     2881 caatgatttc acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat
     2941 caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa
     3001 aagtaatatc gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc
     3061 ctcactaatt gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac
     3121 acagccgatc ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg
     3181 gaaagtcaaa gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt
     3241 agctgaacgt attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag
     3301 cgtgctatcc catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac
     3361 gatcaatgat atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc
     3421 gattttgaca cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc
     3481 aaacgaaatg gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg
     3541 agtagacttt tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt
     3601 gcatgatgtg acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt
     3661 ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga
     3721 aggtgcgtgg aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac
     3781 agaccgaatg attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa
     3841 tacccagctt caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg
     3901 gtttgatatg atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca
     3961 aagagatctt tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat
     4021 gaataacgcg atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac
     4081 gcataataac gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct
     4141 gaaccgagtg ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg
     4201 gacaggactt ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg
     4261 ggcagagagc aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta
     4321 tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg
     4381 atggttgcgt taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt
     4441 tcaatcaacg tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact
     4501 tccgaagtct ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc
     4561 aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag
     4621 ctttctgaag tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct
     4681 ggttttgagt tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat
     4741 ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata
     4801 caattcgatc aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat
     4861 gaagcaaata tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca
     4921 aaatggacag aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat
     4981 tcgatcaaac tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga
     5041 aatgcttttt tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt
     5101 ttttacggtg ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt
     5161 ggagagctgc cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt
     5221 agcaaattag gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc
     5281 gattatcgga tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt
     5341 ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg
     5401 aatacgattc aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa
     5461 atcatccagc agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt
     5521 ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg
     5581 tatgtgaaat ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga
     5641 ttggaggcga attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta
     5701 gggttgaatg tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc
     5761 attcgctcta atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac
     5821 acggggaaga tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat
     5881 cttagcactt cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt
     5941 gtgtttttag acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac
     6001 gtgatcgatc cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat
     6061 attttattcg gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca
     6121 ctagaaatcc aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa
     6181 atcgttcttt ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg
     6241 catttatctg atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca
     6301 atcaatacat tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag
     6361 ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat
     6421 gtagcgatcg aaacaagcga taaagtggtc caaattgaga gcatcaacgc tttcagcaat
     6481 acgatcgtga caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat
     6541 gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct
     6601 tttaaaatca gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa
     6661 aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct
     6721 caaattgatc ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat
     6781 catatacatg gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag
     6841 cagacttggg aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta
     6901 tttgagatgg ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc
     6961 catgacgcag cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg
     7021 ttgacggaca ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt
     7081 tatttgatgg aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat
     7141 ttaaaacagc gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc
     7201 atgactgatg tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa
     7261 aacaatatga aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt
     7321 ggcgttggac atgattttga aatctatgat acagatccag atgaagcaac tgagctattt
     7381 tcgatttaaa cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt
     7441 tggttttatc ttctattttt atttttttag catattaaaa aaataaccgt attatcgact
     7501 gactacactg atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat
     7561 cgcgtaatct gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc
     7621 acttaacaaa taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat
     7681 ataagtccaa tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa
     7741 agctgttgga tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc
     7801 tgtatctaag aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc
     7861 aagcatctca atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc
     7921 gttgaaagca tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa
     7981 atccttttgt ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt
     8041 cacgtctaaa tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc
     8101 tccaatgagt gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt
     8161 tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg
     8221 ttcgtgatca ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag
     8281 gagataagaa atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt
     8341 gaatcttaga cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat
     8401 tgttggctcg ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat
     8461 agacaaaaca ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat
     8521 agccttttgg atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg
     8581 cttaccagtc ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac
     8641 tcagatagat actgtctgga caggtgctga agggatacta ggagcagtta caactgggct
     8701 catcatcgga tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga
     8761 gaagatacct gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt
     8821 cacttttacg cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca
     8881 aggagtatgg acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt
     8941 gcttttatta ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat
     9001 ggtcaatggg ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt
     9061 agcctcagga caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct
     9121 cgatcagttc atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac
     9181 ggtttttgcg aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt
     9241 tttcaacatc aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc
     9301 tgttcctttt attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt
     9361 tggattcgtt cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc
     9421 gggtttctta gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag
     9481 ttttttcatt tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag
     9541 acagacaaaa aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct
     9601 gtcgctttag attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt
     9661 atactagccc tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt
     9721 gtgtgatcat cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac
     9781 ctaggaaaaa gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc
     9841 tgatcactgc tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc
     9901 ggaatacgaa ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg
     9961 cttccatgat catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca
    10021 atacagattc taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca
    10081 atccataaat aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg
    10141 gttcacgttg cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg
    10201 cagttgctgt gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg
    10261 gtgagataaa aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca
    10321 tgtaattcct ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc
    10381 aattatatca taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa
    10441 agcttgcaaa aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt
    10501 aagagtgcta acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta
    10561 ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt
    10621 ttagcttctg ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc
    10681 cgtttgttag aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg
    10741 ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca
    10801 ggaaaagata tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata
    10861 atcaatgagg tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag
    10921 ctatgttacg cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag
    10981 gccgaaatgt cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga
    11041 cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat
    11101 cagaagtagc atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc
    11161 tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag
    11221 gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct
    11281 caactgttgt tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg
    11341 ataaagttgg acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca
    11401 caatcgaaga atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg
    11461 atcgtggtta cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag
    11521 aaaacccata tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat
    11581 tgttagaaca aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg
    11641 gtgaagcatt gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg
    11701 taaaagctcc tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga
    11761 caggtggtac agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa
    11821 acttaggaaa tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt
    11881 ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa
    11941 caacttctga ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg
    12001 tagctgttgt taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca
    12061 ttgaagatgc attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg
    12121 gtacagctct agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg
    12181 caacaggtat caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa
    12241 acgctggtta tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg
    12301 gtttcaatgc tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa
    12361 ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga
    12421 cagaagcagt cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc
    12481 catcaatgat gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt
    12541 agtgaaagac agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga
    12601 aattcacgga aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc
    12661 ttctgcttcc gctgtttata cgtgtttaga
//
LOCUS       DAGCXG010000074        12500 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGCXG010000074 DAGCXG010000000
VERSION     DAGCXG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.077"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..88
                     /locus_tag="MB022_002010"
     CDS             <1..88
                     /locus_tag="MB022_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4466998.1"
                     /translation="ILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            216..1430
                     /locus_tag="MB022_002011"
     CDS             216..1430
                     /locus_tag="MB022_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBT4466999.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            2419..2628
                     /locus_tag="MB022_002012"
     CDS             2419..2628
                     /locus_tag="MB022_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBT4467000.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2740..4353
                     /locus_tag="MB022_002013"
     CDS             2740..4353
                     /locus_tag="MB022_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467001.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4388..5599
                     /locus_tag="MB022_002014"
                     /pseudo
     CDS             4388..5599
                     /locus_tag="MB022_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5622..6548
                     /gene="glsA"
                     /locus_tag="MB022_002015"
     CDS             5622..6548
                     /gene="glsA"
                     /locus_tag="MB022_002015"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBT4467002.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6756..8216
                     /locus_tag="MB022_002016"
     CDS             6756..8216
                     /locus_tag="MB022_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467003.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8437..10200
                     /locus_tag="MB022_002017"
     CDS             8437..10200
                     /locus_tag="MB022_002017"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBT4467004.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10284..10742
                     /locus_tag="MB022_002018"
     CDS             10284..10742
                     /locus_tag="MB022_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBT4467005.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10851..11534
                     /locus_tag="MB022_002019"
     CDS             10851..11534
                     /locus_tag="MB022_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBT4467006.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11681..12295
                     /locus_tag="MB022_002020"
     CDS             11681..12295
                     /locus_tag="MB022_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBT4467007.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12311..>12500
                     /locus_tag="MB022_002021"
     CDS             12311..>12500
                     /locus_tag="MB022_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467008.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLF"
BASE COUNT         4318 a         2014 c         2635 g         3533 t
ORIGIN      
        1 tatcctacac tttgaaaaat acgattatcg ttttcattgg aatagtgata attcagttat
       61 tgaaatttgg atacctattc aagattagaa gcaattttat atccatacac acaaagcagt
      121 tagtcttagg attgactgct ttttctatac agatttttaa tgacaggcaa ttatcaatta
      181 tggtagttgc cttttttgat tggaggaatt gaagaatgaa tgataaaaac tttattgaag
      241 aactaagaca aaagcgtgag gaatacggag taacacaaac aaggcttgct gttgcctgtg
      301 gtatcagccg tgaatattac aaccgcattg aaaaaggaaa acagccattg aatgacgaat
      361 taagagaaat catagaaaaa cagattgagc gtttcaatcc gcaagaacca ctattcttat
      421 tgattgatta ctttcgtgtc cgctttccta ctacggacgc attggcgatt atccgtgatg
      481 tattacaact gaaatctaat tacatgcttt atgaagatta tggaaaatac ggatatgaaa
      541 gcaaatatgt acttggcgac atcaatatca tgtgttccat gcaggagcat ttaggtgttt
      601 tattggaact gaaaggcaaa ggctgtcggc aaatggaatg ttatctgctg gcacaggaac
      661 gctcatggta tgacttcatg ctggattgta tgacggctgg tggtgtgatg aaacggcttg
      721 atttggctat caatgataaa gcaggaatat tggatattcc aaagttaaag gaaaaataca
      781 aggctggcga atgtatgtcc tacttccgta tgcagaaaga ttacagcggt acagaaaaat
      841 gcggtagcga tttaccaaag aatacaggag aaaccttata tctcggttca acaagtagcg
      901 aattatatat gtgtgcttat cagaaaaatt atgagcagta tgtcaagaat ggcacagaaa
      961 ttgaagatac ggagattaag aaccgttttg aaatacgcat gaagaacgaa cgagcctatt
     1021 atgcggttgt agatttactg acctatcggg acgctgaacg caccgctttt tctatcatca
     1081 atcattatgt ccgctttgtt gacagagagg acgacaagcc aaaaagtcaa tggataacaa
     1141 atgatgattg ggcatggttt gtaggggaaa acagagagcc gatacgctta accacaaagc
     1201 cagagcctta cactttacaa aaagcgttgc attggctaca aagacaagtc gcaccaacca
     1261 taaaaatggt acaggcatta gacagagaaa atcgtacaac gatactgaaa gatatgattg
     1321 agcaggcaga actaaaagac aagcataaac atttattaca attagagaaa tcaaccatag
     1381 aagaacgtat agataccgct gttccacaag agaatgacgg tattttttaa tgtaattgaa
     1441 aaatttcaga tttagtcagc aaagagctgt tttcattccc tatgtgaagt gaaagaagaa
     1501 aaataaattt tttattaaat ctgcaacaaa atggctactt gcgtacagat atggtgcgag
     1561 aaaaggaaat ctaaactttt tttgaaaata ggtcattaaa tcggggcttg ctgtccctat
     1621 atgaagtgaa agaagaaaaa agttttgaaa aaatgacgga aattcgtgat tagtggacag
     1681 tcttctgtga aaagaggaaa aacatcatca ttttgtattc aaacaggcat tagcagttga
     1741 gtgttgatgt gaaaagatga aaatactttg ttctcaactt aacaatttaa ggtttccgtt
     1801 ccctatatgg tgcaaagaaa atatttcatt ctattgttgg cagtaacggt atggtttggg
     1861 tagttagggt gtgaaaagga aaatattctt ttttcatcat caacttagca aaatgacatt
     1921 ttctatccct atatggtgca tgaaaagaag ttaaaatttt tccatttaaa cttagcaaat
     1981 ggacgtttgc tgtacagata gtagtgaaaa aggagaaaat caaccgttga ttgccaaaat
     2041 gaaccgttcc atttgtacta ataatggaaa aagttttgga ttttggttac gtttctcctc
     2101 ttttccaacg cgatatatag gaaagacgat ttttttcttt caatcgttct ttgacaactg
     2161 aataaagcta ttcaatacgt ttgacagaca gctagccgtt gaaacaaata cgccacgacc
     2221 tttcccctgc aagagcgagc gaaaacctat ttgtgttctt gtaaaagatt tgtcctcttt
     2281 tatcgccatg acctgtactg tcagataatg atactcccgt acagccacag cttgagcatt
     2341 aagagcgtcg cacgcaatgg gtacggctac ataagaacta tgcagaggtg gaactcctgt
     2401 gggctatgac aaaagccgtt gaattgctta gaaagatttt acagaaaggg ggtatgtgtt
     2461 attgataatg ctgtaaaaac aattcaaaag aaaaatggca agtgtggcat tggcaacaga
     2521 ggaaagacaa agattgtagt aaaagagcat ttttccgaaa agggcaaaac aatggaagaa
     2581 cttctaactg atgtcatgct ggaaaaagca aagcagacaa tcgcataaaa tcggtgcagt
     2641 tgtaagaaat agattgaatg acaaaaagta cccgtgttat actttacttg caagcaggta
     2701 ttgtaaacac gggttagtta taaaggagga taactgacaa tgaaacaaca gatttacaat
     2761 actgcacttt atcttaggtt aagccgagat gatgaattac agggcgaaag ttccagcatt
     2821 accacacaga gaagtatgtt gcgtttatat gcaaaagaac atcatttgaa tgtcattgat
     2881 gaatatattg atgacggctg gtcgggaaca aattttgaca gaccgagttt tcaaagaatg
     2941 attgaggata tagaggcagg aaaaatcaac tgtgttgtaa cgaaagacct ttcccgtctt
     3001 ggcagaaatt atattatgac aggacaatat acagaattgt atttccctag tcataatgtc
     3061 cgctacatag cgattgatga cggtgtagac agcgaaaaag gcgaaagtga gattgcacca
     3121 tttaagaaca tcatcaatga atgggtggca agagatacaa gccgtaaagt caaatcagca
     3181 tttaagacaa agtttgcgga gggggctcat tacggtgctt atgctccgtt aggatataag
     3241 aaacaccctg acatcaaagg aaaactgttg gttgatgatg aaacaaaatg gattattgaa
     3301 aaaatctttt ccctagccta tcaaggttac ggaagtgcaa aaatcacaaa gcagttaaga
     3361 gcagaaaaag ttccgacagc gtcatggcta aattttacaa gatacggtac ttttgcacat
     3421 atctttgagg gaaaacccga aagtaagcgt tatgagtgga cgattgctca tgtcaaggcg
     3481 atattgaaaa gtgaggttta tatcggaaac agtgttcaca atatgcagtc tacggtatcg
     3541 ttcaagagca aaaagaaagt gcgtaagccc gaaagtgaat ggtttcgagt agaaaacact
     3601 cacgagccga ttattgacaa ggaagtgttc tatcgtgtgc aggagcagat aaaatcaaga
     3661 cgcagacaga caaaggaaaa ggcaacgccg atttttgcag ggcttgtcaa gtgtgcggat
     3721 tgtggttggt ctatgagatt tggaacgaat aagacaaata aaacgcctta cagttattat
     3781 gcttgcagtt actacgggca gtttggcaaa ggtaattgtt ctatgcacta cattcgttat
     3841 gatgtgctgt atcaagccgt attggaacgc ttgcagtatt gggctaaggc agtacagcag
     3901 gacgaagaaa aggtattgaa caagatacag aaagctggca atgcagagag aatacgggaa
     3961 aagaagaaaa aggcaagtac attgaagaaa gccgagaaca gacaaaatga gattgaccgt
     4021 ttatttgcga aaatgtatga agatagagcc tgtgagaaga taacagagcg aaattttgtg
     4081 atgttgtcca gcaagtacca aaaagaacag atagaactgg aacagcagat aacaagccta
     4141 agagaagaac taagtaaaat ggaacaggat atgataggtg ctgaaaagtg gattgagtta
     4201 atcaaggaat attccgtacc aaaggaactg acagcaccat tattaaatgc catgatagaa
     4261 aaaatcctca tacatgaagc aacaacgaat gaggataacg aaagaataca ggaaattgag
     4321 atatattacc gatttattgg aagagttgag taatcaacaa gagtaatatt tttaactaag
     4381 ggaaaccggc ggtgtctata cttgggtcag tcgcacattg ggaaaacgat tcggttttgc
     4441 cgcaatcttt ttccaatggt ttgagataac agttggctac ttgaccatgc tgtatttctt
     4501 aactggtgca ttatcctatg caacgggtat tggtgctata caaaacaata aatatctgaa
     4561 attggcaatc ctattgatta ttttctgggc tattttactc tcacagttaa aaggaaccaa
     4621 atacacttca ttgattgctc ggatcggttt tgtagctggt atcttgttgc cagcattagt
     4681 cttgtttggc ttagggatcc attatgtcgc ttcaggcgct ccactacaaa cgatactgtc
     4741 tatgaaaaca ttgattcctg atttttcgaa attgcctacg ctggttgttt ttgtttcctt
     4801 tattttagca tatatgggcg tagaaacatc tgccagtcat gcaaatgaaa tggaaaaccc
     4861 taaaaaggat tatccattag caatgtttgt attggttatt ctggcaatca ttttagatac
     4921 ttttggcgga ttgaccgtcg ctactacagt acctcaacat ggtttgtctc ttaatacagg
     4981 ggtgattcaa tcgttagaat tcctattaag acacattgat ccaaatcttg catggattgc
     5041 gaaaattctt gcgattcttg tttgtttagg tgtcatcgga gaaattgctt cttgggtaac
     5101 ctctccttca aaagcacttc acgtagcagc agaagaagga ttacttcctg aatattttgc
     5161 aaaagaaaac acacacggtg ttcctgctca tttgatgatc gcaaatggga tcgttgcaac
     5221 aatctgggca gccgtcttta cattaagcgg cggggggaat aatctttcat ttcttgcagc
     5281 gatgtcattg actgttgtaa tctacctgat gttgtacttt atgtttttcg cgggctattt
     5341 gaagctggtt tttaaagatg acagtcttga aagagcctat caggttccag gcggaaaagt
     5401 tgtaaaagta atcgttgctt gtttaggtct tttgacttca ctttttgctt tttgtatctc
     5461 atttgttcca cctgctagta tcgcagctgg acagcatggg atttatgaaa cgattctgat
     5521 tgtttcttat cttatcgtct tagttattcc atttacgatc tacgcatgcc gtcataaatt
     5581 ccagcatgca gaaaaataaa aaatgaaagc tggttattca aatggaagta aaagaattag
     5641 aacaattgat tggaaaaaat ttccactatt atcaaacagg acaagtggcg gattacattc
     5701 cagcattagc tgaagtggat cccaaacaat taggtatggc gatttatgat ttggatacaa
     5761 aacaactgat tgagggagga gacgccgatg tgcgctttgc catcgaaagc atgtcaaaag
     5821 ttcccgtatt gttattagca atcaaagaca acggaataga aaaagtcttt caaacaatca
     5881 acacggaacc aaccggtttt gccttcaatt ctatcatgaa tatggaaatc aatcatcgca
     5941 aacatccaat gaatcctttt gttaatgcag gagcgattgc aacaacctcg ttgattgccg
     6001 gtaaagatgc agatgaaaaa tttgagcgaa tcttgtcatt tatgaaagag atctgtgatg
     6061 atcctaatat cacattgaat gaagaaatct atcattctga atcaagaaca ggtgatatca
     6121 atcgctctct tgcttactat atgaaaggta atcaaatgat cgaaggagac gtaccggaga
     6181 tactcgatgc ttatttcaga caatgctccc tcaatgtcac cgctacaggc atagcaaaat
     6241 tagctgctgt gctggcaaat aaaggaattg ctccttggaa tggcaaacaa atcattactg
     6301 aagaaagtgc tacaatcgtc aaatcaatta tgacaactgc tggtttgtat gatgaatcag
     6361 gagagttttc cgtccatgtt ggtgtacctg caaaaagcgg tgtaggcggc gggttgatgg
     6421 cagctgtgcc aaatcgttac ggcataggtg tcttcagccc ggcactagat ccatttggta
     6481 acagcgcagc tggtatccaa ttgttgaaag atgtagtcaa agaactagac gctgacatct
     6541 ttgaataacc attcttgaaa gaggctgggg taaaagtcca aacggctcaa agctacctac
     6601 aatgaggctg aaattgtgag cttgtttgac ttttgtccca gtttcttttt ttattggttt
     6661 ttgaaagggc ttttctaaat aaaaaggaaa caaaaatacg atttacagat atgattaaat
     6721 aaaatcataa gaaaaactgg aggcgtgtat caggaatgct ggaaaattac atcgagcgaa
     6781 atatatttag aaaagtgtat ctgtgtgaac aattatttga attccaagaa atagacatcg
     6841 aacaaacagc tatttctcta agggtgacta caccaacgat ccttcacgat ctagaaagtc
     6901 tagccgagtg tctagaatat tgtataaaag aacaagtaag agagaagcat aagtataaac
     6961 tcgtattcaa acatggtatt gctctttctg agctcacaca gtttctgtac ggtcaatctt
     7021 attttttaaa gtttttaagt tattatttta atggtatatt cacgagtaca gaactggctg
     7081 atcttgaatt tatttctttg agtaaagtat atacaatcaa aaaaatcgta ttggattttt
     7141 ttaaagaaaa tgattatttg aaaaataaag aaatcattat cccagaattt gactcgcgga
     7201 acatcttgct agctttagta cgttatgtca attgggaagg ctatgaaaat aaaaatccgt
     7261 atgtagaaga agcttgtaac gagttgattg attatgtaga gactcatttt tttaaaagaa
     7321 gatattcgga agatgaaaag tcctttatca tacgagaaat agagatcgca atcggaagaa
     7381 aggcatttcc ccttggactg aaagaagaag ataagaaaag cgcagaaacg aagccgttat
     7441 ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat tctagactta caagaagaag
     7501 atgtctatta tattttttct ttgttcaaca gtagaaatta taccaatgag aacatggagc
     7561 tgttaagaaa agatttcgaa gttgtttata aaaattttgt tctcaatgac ctatcactga
     7621 atgaattgat tttacagatt attagtatcg ctgaaatcga cgatgcagaa aattttattt
     7681 tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc agatggacaa gtatttttac
     7741 cggaaaggat ctatcttcta tcagaaaaag aacaacttct gtacggaaaa attgtagaaa
     7801 tattggaaga gtggaaaaag aaaaatcaaa tccaactaag atggaatgaa aatttcattc
     7861 gaaaattcgt caaaagtctt tctttgatca acagtgaaca agcggggaga caggaaatag
     7921 aaatttttgt tgtttcagaa agttcagtca aacaattttt ttatcgaacc caatttcatt
     7981 tgtatgcgga agaaggcatc gctgaaataa atcctatttt atttcgttca ttagaagaat
     8041 tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt gtgcgatgct gctagctatc
     8101 aaaatggatt agaaactgaa aagacaaaga tctatccaat ctcattaaaa ggtgcaaaca
     8161 tccatttgta tcatattgct caggaacttc acctattaaa aaaacatatt tcttgatgag
     8221 accttaatta ggagaccttc tacaaagctt tttaattgtc tgtagaaggt ttttatttga
     8281 gtcctgattc gtgcgaagat ctacttatat aacaagcttt ctctgaaatt tttttaaaat
     8341 gctattgact tagagtcaac tctaagtagt agaatagata aagtgtaaca aatctatttt
     8401 cattaaaaga agatagtacg gaggagagaa tgtattatga gtacagtatt acatgtattg
     8461 gatgcaatca atagccccgc agatgtcaaa aaattatctg ttgaggaaat gaaacagttg
     8521 gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg atgtaggtgg gcacgttggt
     8581 ccgaatctag gtgtagtaga attaacgatt gcgtttcatt atgtatttga ttcaccaaag
     8641 gataaaatcg tttgggatgt ttcccatcag tcttatggtc ataagctttt aactggtcga
     8701 aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg gatattccaa ccaacatgaa
     8761 agtaaacatg actattttac agtggggcac acatctacat ctatcagctt agcaacaggt
     8821 ttagctaaag cgagagatct gaagaaagaa tcaggaaatg tggtagcatt tatcggtgac
     8881 ggctccctaa gtggtggctt agcttttgaa ggattgaaca atggtggtgt tttacaatcc
     8941 aatctaatcg tattagtaaa cgataatgag atgtctattg atgagaatca tggcagttta
     9001 tataaaaact tagctgagct tcgtcaaacc aacggtaaaa gcagcaataa cctatttaaa
     9061 gcaatgggat ttgattatcg ttacgtggat aatggaaacg atctggaaac ggtgatccgt
     9121 ttatttgaag aagtaaaaga tattgatcat ccgattgtac ttcacgtcca tacggaaaaa
     9181 ggtcgcggtt atcagccggc aatcgataat aaaatgaaat atcattggca tgtaccattt
     9241 gatctgtcca ccggggaggc aaagcaagta acttctggag aaacatttaa tatgatcatc
     9301 atggattatt tggataagaa aatgcaaaca agtacagctc ctattgccgc agtcaatgca
     9361 gcgataccag gcgcctttgg actgaaaaca ttccaagaaa aacatccaga tcattatttt
     9421 gacgtgggga tcgctgaaca gcactctatc actttcgctt ctgctttggc ttctcaggga
     9481 gtacgtccag tcgtcttcca atcaagtact ttcttgcaac gggcatatga tcaattatca
     9541 catgatttag cgatcaacga gaatccagtg attattttag tcggtggcgg aacgatctca
     9601 gctagtgacc gaacacacca aggaacattt gacattcctt acttatcaag tatcccgaac
     9661 atcaaatatc ttgctccagc aacaaaagag gatatgatct ctatgctaga ttgggcgttc
     9721 gaacaaacag cgcagcctgt agcaatccga attcctacac atggtgtaga acatggtaca
     9781 gctatcgcaa atgattatag tattccagcc tatcaaacag taaataatgg aggaaaagta
     9841 gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag aaacagtaaa agcttttaga
     9901 aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga tcacagaaaa tgacgtagaa
     9961 acattagata aattaaaaga aacacacgaa ttagtcatca cattagaaga tggtagtttg
    10021 tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg attcttcaat gaacgtatta
    10081 aattttggtg cagagaaaga attcacggat agcgttcctg tagggcagct ttatgaacgt
    10141 tatcatttaa caccaaaaca aatcgttgaa gatagtctgc gtgtcttgaa tattcaataa
    10201 ttcattatgc tcaccatcca gataattgtt tatactagta tctggatggt tttttcatta
    10261 taaaaagcaa aggggtggat gttatgtatc agcaagtaga agaatatgtc aaaaagttcc
    10321 aagaaaaata tcctagttat caatcagaaa aattaggttt cttttcattt ggggatacag
    10381 aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa gataaagact ggtacatcaa
    10441 gtggttacga tctgtataaa caagagaatg agcctttacc aaaagttggt caattagacg
    10501 tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa aaatataaat gtgaccatct
    10561 tgccttttag tgaagtcacg aaagagcatg cttggaaaga aggagaaggg gatcggacat
    10621 tagcttattg gcggaaagtc catgctgagt tttttaaaca tgcttataaa gaagcgttga
    10681 acatagaatt tacagaagag aagctggtcg tatacgaaga atttgaagta attttctttt
    10741 agtaagaaag gtctgctgaa agaaatagct cattattgtc tcctgaatag aaaagggtta
    10801 catttttata tgaagctgta tttttatata acattactgg aggagatatt gtggatacac
    10861 tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc tttggaaaac gagccgctac
    10921 acagttcttt tattatgggg agaatggctt tggctgcaaa agaaggcgga gcagttggta
    10981 ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg aatcgttgat ttaccggtaa
    11041 tagggattat caaaagagat tacgatgggt cagaagtgtt tattactgcg acagaaaaag
    11101 aagtggaaga acttttggag acgcaagcag aaatcattgc actggatgca acttctcgta
    11161 aacgtccgac agaaaaaaca actgctgaac ttgtccaaat gattcacgaa cacggtcggt
    11221 tagctatggc agatatttcg acttttgagg aagcgcttca agcgcaagaa gatggttttg
    11281 atcttttgtc tacgacttta gcaggctata cagaatattc acgaaagacg cagacacctg
    11341 attttgattt attgaatcaa atcatagaac aagtcgatat cccagtaatt atggaaggac
    11401 atactgactc tcctgaacaa gtagagaaag catatgaatt aggtgctttt tcagtcgtag
    11461 ttggatctat tatcacccgt ccgcaattga tcactaaaag atatgtcgaa gccgcaagta
    11521 aagcaaataa ctaaaaaaca cgcacggcac agattcttga caaaaaagaa aaattgatag
    11581 atagtttaaa gcagattcta agaagatgaa aaaagcatta cttttcattt tgtcttcaag
    11641 atctgctttt taacgaattt tatagaaaga aggcgaaacc atgtttgaac ggagtccttc
    11701 agaattgaca gaacgtgaaa attacaagtt gctgattggt tcagtcatcc ctagaccagt
    11761 agcagtagta acgactaaat cagcaaaaga tatagtgaat atcgcaccct tcagttactt
    11821 caatatcgtc agttcgaatc gacccatctt atccttggcg atccagagaa aacaagatgc
    11881 agtaaaagac acagcaaaga atattcttga gacaagagaa gctgttatcc atatccttga
    11941 tgaggacaac gtagaagaag taaataaaac agcagccaat ctgcctaatg aggaaagcga
    12001 actgtacctg actgacttga ctttaactga ttcagtaatc atttcagtac cagcattgac
    12061 agaagcaaaa gttcgttttg aaacatcctt atatgaacat gtcgagatac ggaataaaaa
    12121 taaagtaaca gctgatttat ttttgctaga agtacagaaa tatcatttaa atgaaacagt
    12181 ctatgatgat aagacaggaa ggatcgatcc aagaaaattg aatgctgtaa gccgtttggc
    12241 aggaaatgat tatgcagcaa taggagaaat tttcacgatt gcacgtcccg aataaaaagg
    12301 agcactagtt atgttaaaaa taagctataa accatcgaca gattcaaaag aaatgaaaaa
    12361 agaatatgaa acagtgaatg attttctgca agggcagtac ttagaagttc cgccattgca
    12421 agaccatttt gtcgttacta ctgttacttt agatggtaag gaaatagaaa tgccagatca
    12481 aacgattagc ggattgttta
//
LOCUS       DAGCXG010000075        12363 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGCXG010000075 DAGCXG010000000
VERSION     DAGCXG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.078"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(64..621)
                     /gene="frr"
                     /locus_tag="MB022_002022"
     CDS             complement(64..621)
                     /gene="frr"
                     /locus_tag="MB022_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBT4467009.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(624..1346)
                     /locus_tag="MB022_002023"
     CDS             complement(624..1346)
                     /locus_tag="MB022_002023"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBT4467010.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1482..2363)
                     /locus_tag="MB022_002024"
     CDS             complement(1482..2363)
                     /locus_tag="MB022_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBT4467011.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2462..3244)
                     /gene="rpsB"
                     /locus_tag="MB022_002025"
     CDS             complement(2462..3244)
                     /gene="rpsB"
                     /locus_tag="MB022_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBT4467012.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3603..4082
                     /locus_tag="MB022_002026"
     CDS             3603..4082
                     /locus_tag="MB022_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBT4467013.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4299..5390
                     /gene="phnW"
                     /locus_tag="MB022_002027"
     CDS             4299..5390
                     /gene="phnW"
                     /locus_tag="MB022_002027"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBT4467014.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5383..5508
                     /locus_tag="MB022_002028"
                     /pseudo
     CDS             5383..5508
                     /locus_tag="MB022_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
     gene            5540..6169
                     /locus_tag="MB022_002029"
     CDS             5540..6169
                     /locus_tag="MB022_002029"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBT4467015.1"
                     /translation="MQQLLSLPSVKEQWIAIYGQLPQEKEVEELYQQFEHYLFKDLAQ
                     HSTLKPDTLETIERLRNAGINIGSTTGFTAPMMAIVAETAKKEGYSPDFIATSEDVEG
                     YGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISY
                     DEYQKLEMGKKDRLKQSVALRFQQVGADICVNNLKELTEYILYADKNPE"
     gene            complement(6759..8450)
                     /locus_tag="MB022_002030"
     CDS             complement(6759..8450)
                     /locus_tag="MB022_002030"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBT4467016.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8872..9819)
                     /gene="arcC"
                     /locus_tag="MB022_002031"
     CDS             complement(8872..9819)
                     /gene="arcC"
                     /locus_tag="MB022_002031"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBT4467017.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9934..10953)
                     /gene="argF"
                     /locus_tag="MB022_002032"
     CDS             complement(9934..10953)
                     /gene="argF"
                     /locus_tag="MB022_002032"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBT4467018.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(11044..12273)
                     /gene="arcA"
                     /locus_tag="MB022_002033"
     CDS             complement(11044..12273)
                     /gene="arcA"
                     /locus_tag="MB022_002033"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBT4467019.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3394 a         2578 c         2245 g         4146 t
ORIGIN      
        1 aaaagaaata taaataaaaa cagaggaagc ggaatgatcc gcccctctgt ttttatgatt
       61 ttattaaact tccaataatt ctttttcttt atcagcagcg atcttatcta cttctttgat
      121 gctgttatct gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat
      181 atcaccattt ttttgttgtt ttttatattc atcgatcgca tcacgacgga tgttacgaat
      241 cgcgattttt gcattttctg cttctttttt aacatcttta gctaattctt ttctgcgttc
      301 ttctgtcagt tgcgggataa ctaagcgaat gacatttcca tcattggttg gactgattcc
      361 gatatcactt gccataatag ctttttcaac atcttgaaga atgctcttgt caaaaggtgt
      421 gatcattaaa acacgtgctt ctggaatttg gattgatgcc atttggttca atggtgttgg
      481 cgcaccgtag tagttcacag taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg
      541 gatttgccca agttcacgtt gaagattttg tgctgctttt ttcatttttt cttttgcttc
      601 tgtcataatt gcatctgcca tttttatttc ccccttacag ttgttccgat attttcgcct
      661 aagattgcac ggcgaatatt tccgtgttca tttaaattga agacaagcag tggaatatca
      721 ttatccatgc ttagtgaact tgcagttgaa tccatgactt gtaatccttt ggcaataact
      781 tccagatgag tcagttcttc aaatttcact gcatttgcat ctactttcgg atcagcagaa
      841 taaacaccgt ctacattgtt tttagccatc aggataacat ctgcgccgat ttctgccgcg
      901 cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata
      961 acgatccgtc ctttttctaa atgacgttct gctttacggc gaatatatgg ctctgcgatt
     1021 tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc
     1081 tgtaaagcta aggcattcat aactgtagct aacataccca tgtaatcagc ttgagcacgt
     1141 tccatgccca tttgtgctcc gatttgtccg cgccagatat ttccgccgcc cacaacgatt
     1201 gccatttcaa cgcccagctc gtgaacttct ttgatttctt ggatgatttc tttgattact
     1261 ggtggtttga ttccaaatcc tgcatcccca gctaaagctt ctccactcaa ttttaagact
     1321 acacgctgat atttcggtgt gaccatgttc attcctccaa tattttctat tgccatttta
     1381 ccatacttag atgatttcga cactctaaga ctggtttttc ttgttttgct taaaaaaagg
     1441 gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga attatttttt cacttggctc
     1501 ataacttctt ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt
     1561 acgaatgttt taacagttgc gccttttgat gcaacgtatt tttcaacagt catatcagga
     1621 tctttaacga atggttggtc caccaaagcg atttctgctt tgaatttctt caagcgtcct
     1681 tctaccattt tttcaacgat attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga
     1741 acagtttttt catgttctaa ttcagcttct ggaatttgtg attcgtttac ataacgagga
     1801 ttgatagctg ctacgtgcat agctacgtct cttgcaactg tttcgtcagt tgttccatct
     1861 agaacagtca atacggcaat acgtccgccc atatgcaagt atccaccaaa tgcagcattg
     1921 tcgtcttttt ctacaacttc aaagcgacgg aagctgattt tttctccgat aacttgagtt
     1981 gcttcgatca agtcagattc aattgtacct ttatctgttt tgattttcat tgcagcttcc
     2041 atgtctgctg gtttgttttc agcaacaagt tctgcaattt cttttactaa atcttggaac
     2101 atttcgtttt tagaaacaaa gtctgtttct gagtttactt caacgattgc tgcagtgtta
     2161 cctttcacag caactgaagc caatccttca gctgcaatgc ggtcattttt cttcgcagcc
     2221 tttgccattc ctttttcacg taaaagatca actgcttttt ccatgtcgcc ttctacttca
     2281 actaatgctt ttttcgcatc catcattcct acgccagtca tttcgcgtag ttcttttacc
     2341 attttagctg taacgtctgc catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa
     2401 gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa
     2461 attattctgc tgaagaattg tcgccttcaa caacatctac gatttcttcg attgatgttg
     2521 ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg tttccttcga
     2581 tgaaagcatc agccattttt gaagtaatca atttaacggc acgaattgca tcgtcgtttg
     2641 aagggattac tacgtcgatt tcatctggat cgcagtttgt atcaaccata gctacgattg
     2701 ggatatttaa tttgtgtgct tcttgaacag caatacgttc tttacgtggg tcaacgatgt
     2761 acattacatc tgggattctt ggcatatcag cgataccgcc taagaatttt tcaagacgtt
     2821 cacgttcttt gttcaaacca gcaacttctt ttttaggtag aacttcaaaa gttccatctt
     2881 cttccattgc attgatttgt ttcaaacggc tgatacgttt ttggatagta tcccagtttg
     2941 tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg tcctgcacga gttgcttctt
     3001 ctttgattgc ttcttgtgct tgtttttttg tacctacgaa taatgctacg ccgccttctt
     3061 cagcaacgct cttcatgtaa tcataagctg catctactaa tttaactgtt ttttgcaagt
     3121 caatgatgta gattccgttt ctttctgtga agatatattt cttcattttt ggattccagc
     3181 gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata gaaattactg
     3241 ccatgtttgt ttcctccgat ttggttttat ttttcctctt cttatcttct accttgccgg
     3301 caaacttttt caagcaccta ccgacaatcc aataagaatg tggatttgct gatgcataaa
     3361 gcaccctcag ctattataca gacaaaagat ccgtatcgca agagcaaata tacttttctg
     3421 ctcaacattc aatagtttac ctgaaagata aaaagaattc aagaaaaaca gtagatttct
     3481 ctttgtttct ttattatcct ttttccataa aataatttta ggtataaaag acgaatatta
     3541 ttgggcaatc cttctttttt ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga
     3601 aaatgcgcaa agctcaaaga catttgctga ttaaacaagt cattgaagaa ttcccgatcc
     3661 gtacgcaaga agaactatta aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa
     3721 cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt
     3781 cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac taggactgag gaagaggatc
     3841 ggctggttca aatgattgaa gacattgtat tgaaagtcga acgagtccac tttttgacga
     3901 tcgtcaatac cttgccagac aatgctcatt tatttgcatc tgttttagat gagatcaaac
     3961 ctccctctat tgtcagtaca attgctggat tcgatacgat catggtcatc tcggaaaatg
     4021 aggaggacgc aaaacgagta gaagaatttc ttcaccatcc aactgaagta tcactttttt
     4081 aatcagagca cgtattccta atatggaatg cgtgtttttt tattttcaat attttcctta
     4141 ttaaataaac ctttacactc tttacaaaaa tacatcattt tttattcaaa tttttcaaca
     4201 ccttgattga atcacaataa tcatcattta tattggatgt taacaagagc agcttcttgc
     4261 tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta ttaactcctg
     4321 gaccattgac gacttctgaa acagtgaaaa atgctatgct gatcgatcat tgtacttggg
     4381 atcaagaata caaagaaatt acacaatgga taagaaaaaa attactggat cttgctcaag
     4441 tatcggaagg aatatacaca acggtcttaa tgcaaggtag cggctccttc ggggtagaat
     4501 cggtattaag ttctgtgatc aggccagaag atactgtact gatttgttca aacggcgcat
     4561 atggacaacg aatgatcaac atgtgcgaat gtctgaaact gaactatacc gtttatgagg
     4621 tatcggaaca tcaaatcctc caagctcatg tgattacaca gctgattgaa gagaatccgt
     4681 ctatctccgc actagcaatg gtccatagcg agactacttc cggtattttg aataatttgg
     4741 aagagattgc tgctgtcaca caagaaagag atcttttgtt tatagtggac gcgatgtcaa
     4801 gctttggtgg gataccgatt cgtgtagccg aactagggat tgattttttg atttccagct
     4861 caaacaaatg tattcaaggt gttccgggct tttcttttgt acttgctaat aaagcggaac
     4921 tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga tctatatgac cagtaccgcg
     4981 tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc tacccatacg gttttggctt
     5041 ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat cgaaactcga tattcacgtt
     5101 atctcgccaa taatcatgta ctacgacaaa aaatgacaga gatggggttc cagcctttca
     5161 ttcaagaata tcaagggcca ttcatcacct cttttcttta ccctaaggac tggcgatttg
     5221 attttaatca tttttatcag tatttaaagc aaaacggatt tgtgatctat cccgggaaaa
     5281 tcagcgatca tgaatgtttt cgtatcggaa atattggcga gatctatccg gaagatattt
     5341 tggaactgac agcaattatc aaagactata aggagaacgt taatgattaa aactgtaatt
     5401 tttgattggg caggaacaac tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga
     5461 aatgcttttc tagaaaaagg aatccaactg actgacaaag aaatccgcga accgatggga
     5521 aaattaaaaa tgggatcata tccaacagct tctttccctc ccttctgtca aagaacaatg
     5581 gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt atcaacaatt
     5641 tgaacattat ctgtttaaag atttggccca gcattctacc ttgaaacccg acacattgga
     5701 aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgactg gatttactgc
     5761 tcctatgatg gcaatcgtag cagaaaccgc aaaaaaagaa gggtattcac cagacttcat
     5821 agcaacttct gaagacgtcg aagggtatgg tcgaccgttt ccttatatga tccataaaaa
     5881 catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag atacggtctc
     5941 agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg aaggtagttc
     6001 gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa aagatcgttt
     6061 aaaacagtct gttgctctta gattccaaca agttggcgct gatatttgtg taaacaattt
     6121 aaaagagtta acagaataca ttttgtacgc tgacaagaat ccagaatgac ttttatacaa
     6181 aataagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
     6241 gctcctcata tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
     6301 tgaatcagag gtcgtgaaaa agctgtcctg catcaagtaa taaaaacagc caaacaaaaa
     6361 tagctcctca tatttttcgt ttgattgggc ttattaccgc agatgcaagc ttttgaacac
     6421 cgtgaatcag aggtcgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa
     6481 aatagctcct catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac
     6541 accgtttatt cgaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg
     6601 caaacaaaca gtgtttaaga gacaacctct ccactaaacg ggtgttcatt cagctgactc
     6661 tcggtatttg ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa
     6721 tactcggatc agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga
     6781 agtcctagca aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag
     6841 cgtgcttctt tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat
     6901 gcttgtgcta atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca
     6961 gacaagaccg tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca
     7021 ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact
     7081 gcacgagcat gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct
     7141 tccaggttaa agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc
     7201 ccaaccccta cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg
     7261 atttgttctt tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt
     7321 cctttacgag ttgataattt tttaccacct tgtgtgatca aaccaaatgg aatatggtgc
     7381 atgtcgtcag accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga
     7441 tagctttgtt cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg
     7501 tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc
     7561 aacgctggat tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc
     7621 agcaaatgtt tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct
     7681 tcaccgttca acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc
     7741 atcgattcat cacggaacca ttgccataat tgaatagcct cttcatcgcc ttcttccaga
     7801 cgtttgaacc aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg
     7861 aattggacat ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta
     7921 ccccattttt tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg
     7981 ttgatgcgga ttggctgata accgattttt tccataatga aaccgatcga attaccgata
     8041 actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg
     8101 attggtactg ttccttgatt cccgatattg ctatccccat aatgttcttt ttctttcact
     8161 actgtttgca aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt
     8221 ccgacaactt cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg
     8281 atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct
     8341 ccgtgttcag ctgattttgg attttctagt aattgttcga cctgttccag tgttaaatca
     8401 tctttgacag cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct
     8461 cctaaagtgc tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat
     8521 gccatcacta gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag
     8581 ctccatctga tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg
     8641 ttgtatcttc ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag
     8701 tactctcttg ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa
     8761 aaacgaaagg ctagtcgttt ctgataaaaa aacaatccag atggcctaaa ggagaagaaa
     8821 taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa attaatcagc
     8881 tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta aagaagtgat
     8941 gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcctcta ctttcggaag
     9001 catgcttcct ggagcaaatt gcttttcatc aatgtattgt ttcatttctg aaacagtaac
     9061 agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt ctactcctgt
     9121 caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag caaaatcttt
     9181 atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag gaatcccacc
     9241 gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga cacgtgcttc
     9301 tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc cagcatcttc
     9361 tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt ccgtgtagaa
     9421 tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca caataacttg
     9481 agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt cgcccatggc
     9541 gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat ctaaaggcat
     9601 tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac cgacttgcgg
     9661 accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca ctaaatactt
     9721 tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag acaagattgc
     9781 attaccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc tttcttcagt
     9841 tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc ctcaatgctg
     9901 actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa acaagttacc
     9961 taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt ggtcaaattg
    10021 acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga tgccaaagcg
    10081 ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca agcagtgcaa
    10141 gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga tttggtaagg
    10201 tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata cccatacatc
    10261 agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa tcatcaattg
    10321 tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt caggttgtaa
    10381 ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga ctaataaaga
    10441 gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt ctactgttcc
    10501 aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc attcatcagt
    10561 caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca ttttttgact
    10621 gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg tatcttctgt
    10681 ggattctttt ttacctaatt ggatatcatt tgcacctaaa tattcaggat gtgcacctag
    10741 atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt caaataaaag
    10801 cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca agtctttcaa
    10861 gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt tttctgctaa
    10921 taagcttctt ccttggaata ctgattcttt catattaaac attctccttt agattgtttt
    10981 tatttaagat taaagaggaa atcccgacgg cagctgacag taagaccgcc gtcaggaaac
    11041 aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac caccacggcc
    11101 tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac gaagcaattc
    11161 gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg ccaatgtgtt
    11221 tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac cagttgggat
    11281 cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt tatctgaatg
    11341 agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct taccgtcttt
    11401 actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa atacggtatc
    11461 caaatgcatc attgcacggt tgtttggaat tttgattgct aacacttttt caaatcctga
    11521 attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag ttcgttgaga
    11581 aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt cgatatgatc
    11641 tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg gatgatgttt
    11701 caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca tcttattgac
    11761 tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt ttggcattgg
    11821 gtccatgtaa aatggatagt catcgtctgc tgacagatcg tatagactgc tagaacgtac
    11881 ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt gcgtttccat
    11941 tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat gagattcatc
    12001 caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt ctgccgctaa
    12061 tttttccaag taaagagtct cgacgccttc attttgcaat gtctttgcaa agttgtcatg
    12121 ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat ccataatatc
    12181 tggtgtcaaa ttttccactt cttgtccagg ccttttcagt aaaactgtct ttaatttccc
    12241 tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc tttttgatta
    12301 catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat tcaaaatgag
    12361 aca
//
LOCUS       DAGCXG010000076        12355 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGCXG010000076 DAGCXG010000000
VERSION     DAGCXG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.079"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(399..986)
                     /locus_tag="MB022_002034"
     CDS             complement(399..986)
                     /locus_tag="MB022_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467020.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1161..2483
                     /locus_tag="MB022_002035"
     CDS             1161..2483
                     /locus_tag="MB022_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4467021.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2638..3414
                     /locus_tag="MB022_002036"
     CDS             2638..3414
                     /locus_tag="MB022_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4467022.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3407..3976
                     /gene="rnmV"
                     /locus_tag="MB022_002037"
     CDS             3407..3976
                     /gene="rnmV"
                     /locus_tag="MB022_002037"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBT4467023.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3969..4853
                     /gene="rsmA"
                     /locus_tag="MB022_002038"
     CDS             3969..4853
                     /gene="rsmA"
                     /locus_tag="MB022_002038"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBT4467024.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5304..5933
                     /locus_tag="MB022_002039"
     CDS             5304..5933
                     /locus_tag="MB022_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467025.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6057..7166)
                     /gene="ugpC"
                     /locus_tag="MB022_002040"
     CDS             complement(6057..7166)
                     /gene="ugpC"
                     /locus_tag="MB022_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBT4467026.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7375..7797
                     /gene="mgsA"
                     /locus_tag="MB022_002041"
     CDS             7375..7797
                     /gene="mgsA"
                     /locus_tag="MB022_002041"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBT4467027.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8120..8656
                     /locus_tag="MB022_002042"
     CDS             8120..8656
                     /locus_tag="MB022_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4467028.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8696..10459)
                     /locus_tag="MB022_002043"
     CDS             complement(8696..10459)
                     /locus_tag="MB022_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467029.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10462..12177)
                     /locus_tag="MB022_002044"
     CDS             complement(10462..12177)
                     /locus_tag="MB022_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467030.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3880 a         2261 c         2502 g         3712 t
ORIGIN      
        1 ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa taaatcctag
       61 aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa taggaatcgt
      121 aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga ttcatctggt
      181 actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta ttacatctat
      241 atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt tatgttcaat
      301 tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc atcggctttg
      361 acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt atggtaacct
      421 tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa
      481 aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt tgtcaacatc
      541 acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg attatcccaa
      601 tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata gatcccgact
      661 aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa
      721 agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat cccccacaag
      781 atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag caatattagt
      841 ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa
      901 tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata atctgctaat
      961 ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa ggtaggttta
     1021 gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc actgaaagtg
     1081 gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat accaaatttc
     1141 aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat ctgtttggcg
     1201 cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg
     1261 ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg ggtggtttct
     1321 taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg gaaactggtg
     1381 gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta ttaggatggg
     1441 cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc tttgcgacac
     1501 aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca gtgatcaccg
     1561 ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct gtacaatcag
     1621 tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga ttattgaccc
     1681 caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg
     1741 aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg
     1801 gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca attattcttg
     1861 gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg aaaactttgc
     1921 cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac gtcatttttg
     1981 gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac ggtgcattga
     2041 acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa gacgagctac
     2101 ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat gttcctgcgg
     2161 ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac tttttaacag
     2221 acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt gtctttattt
     2281 taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat ccaatcgttc
     2341 cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg acgcaaacga
     2401 aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat tatcaaaaaa
     2461 agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt acgaactagg
     2521 tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat tttgattttt
     2581 gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga gataaacatg
     2641 atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat tccagaaacg
     2701 attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca
     2761 acgattgaaa agtctttgca attaagccat gactatccaa atatatacag tattatcggc
     2821 tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt acaagaacaa
     2881 ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca ttggatggaa
     2941 gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc aaaagaaatg
     3001 aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca aatcctaaaa
     3061 gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga ttatgaatgg
     3121 gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt cactttcaaa
     3181 aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa
     3241 acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga accaggctat
     3301 actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga agaagtggct
     3361 gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg ataagatgaa
     3421 gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc gggaagtagt
     3481 tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc tgatgcaaat
     3541 tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt tttcaggaga
     3601 aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt ttttgccgcg
     3661 cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc
     3721 tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg ctgtttctga
     3781 aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag caaaagaaaa
     3841 acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac aattggaaaa
     3901 acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga aaatagtgga
     3961 ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa gagattttat
     4021 tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact gaaccaaata
     4081 ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg
     4141 gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa gtcgtagcat
     4201 ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc tatgacaatg
     4261 tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt agagaaacat
     4321 ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt acgacaccga
     4381 tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc atgatgcaaa
     4441 aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt tcattatcga
     4501 ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa acagtatttg
     4561 tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat ataccggcag
     4621 tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt cagcaaagaa
     4681 gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag acaaaagcat
     4741 ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg gagactttgt
     4801 cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga
     4861 ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     4921 aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca agcttttgaa
     4981 caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa ggaacaattt
     5041 caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg caagcttttg
     5101 accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc ctatttttgt
     5161 ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg atttagaaaa
     5221 gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat ttatgtgtca
     5281 agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta acgatttctg
     5341 ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc atgattccac
     5401 cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt tttacaccag
     5461 gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca acgccggtta
     5521 tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct tggtacttgc
     5581 aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc
     5641 aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta gtagtcagca
     5701 tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat ttttatacag
     5761 tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat aatattgctt
     5821 attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa
     5881 aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat tagacatgat
     5941 ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga tgagagtttt
     6001 ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg ggcaatttat
     6061 ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct gatgttgaag
     6121 gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt tgagatgaac
     6181 tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc ggaaacgaca
     6241 acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg
     6301 tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc aactaatact
     6361 ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat aacaccattt
     6421 ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc aacgaacata
     6481 ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa tccgtctttc
     6541 atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt aacatagatg
     6601 gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc tacacgtaat
     6661 ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc ccgaacgatt
     6721 gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg ttttcgtttc
     6781 aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt gatgtctgct
     6841 ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt catatgagga
     6901 tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc cacgtcattc
     6961 atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc cgcgatcatt
     7021 cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa ttcacggtct
     7081 ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata ttttttatat
     7141 acgtttttca gagccatttc taccataata cgttccacac tttctttttt agttttatta
     7201 actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga acaagaattt
     7261 tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata agaaataaaa
     7321 actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa
     7381 atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat
     7441 aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca gaggatcacg
     7501 gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg ggatcagcaa
     7561 attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg agacccgctg
     7621 gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga tgtctatgag
     7681 attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact ggaagccggc
     7741 ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc attttgatgg
     7801 ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa aagctaaaca
     7861 aactgataat atgcgatata atacgaacgt attggataat ataaacaata atattagttt
     7921 ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa
     7981 taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg cctcttggac
     8041 gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga tgtagcagct
     8101 cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga ttgctattat
     8161 gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg agaccccatt
     8221 tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt ttggtccttt
     8281 ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct tctcgaaatc
     8341 tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct acggattctt
     8401 tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta gtgtcacgct
     8461 gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg tgccattgtt
     8521 cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac cgattcaatc
     8581 aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa gaagtttagc
     8641 gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct tgtttttatt
     8701 gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga agcagctcat
     8761 catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg tccgcatttt
     8821 tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc attgctaaga
     8881 aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct tcatctaaga
     8941 tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg cgttgtccat
     9001 ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc aaacgttcta
     9061 caaattcata catataagag gcttttaggg catcattgat ttctgcgtca cttgcttccg
     9121 gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca
     9181 ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca tggtcatcga
     9241 ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca agtgtggatt
     9301 ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca gtaaaattga
     9361 aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca aaagagactt
     9421 ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc ttttcttctg
     9481 gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg
     9541 tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc gtaaagctgg
     9601 aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg ccaactaaac
     9661 caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc aaggaagaag
     9721 aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca aattgctgtt
     9781 gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt cgttcggtca
     9841 caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt tgtgaagcac
     9901 gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg accaatgtca
     9961 gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc actccagaaa
    10021 ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca tttgtaaatc
    10081 gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat cgattcagat
    10141 gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga ttgcttaaca
    10201 cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat agaataagta
    10261 aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg atactttgac
    10321 caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt atcacagcta
    10381 gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg
    10441 ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt cctgatattc
    10501 tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac cttgatccat
    10561 cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa taatcactgt
    10621 actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat aatccaacgc
    10681 acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt
    10741 cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt ttgtatctaa
    10801 accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag cttcccaaca
    10861 ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag tcccggtaaa
    10921 tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca gctgccaaga
    10981 acgaacatcc attccgtcca ttataactgt accggaactg acatcataga aacgcggaat
    11041 caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa ggactgtatt
    11101 tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag tataacggaa
    11161 gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag tactgacctc
    11221 agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc ctgaagcttc
    11281 ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa gcatttgatt
    11341 catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat ttactttgat
    11401 cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg tagcaggtga
    11461 taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat ttgtgctttt
    11521 atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac gaacaccgct
    11581 aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt tttggtagag
    11641 aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca ataatgctaa
    11701 gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc cgatacttaa
    11761 aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa gctgattgat
    11821 gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga
    11881 gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc cttgtgaagc
    11941 tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag acatagcgac
    12001 catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc cattatcgat
    12061 caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa
    12121 cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact gaatcatatg
    12181 attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac attgtagctt
    12241 aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt attatgaaga
    12301 agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa aacat
//
LOCUS       DAGCXG010000077        12311 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGCXG010000077 DAGCXG010000000
VERSION     DAGCXG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.080"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="MB022_002045"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="MB022_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBT4467031.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="MB022_002046"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="MB022_002046"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBT4467032.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="MB022_002047"
     CDS             complement(2169..2906)
                     /locus_tag="MB022_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4467033.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="MB022_002048"
     CDS             complement(2997..3524)
                     /locus_tag="MB022_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467034.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="MB022_002049"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="MB022_002049"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBT4467035.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="MB022_002050"
     CDS             complement(4401..4886)
                     /locus_tag="MB022_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBT4467036.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="MB022_002051"
     CDS             complement(5039..5236)
                     /locus_tag="MB022_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467037.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="MB022_002052"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="MB022_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBT4467038.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="MB022_002053"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="MB022_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBT4467039.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="MB022_002054"
     CDS             9211..9423
                     /locus_tag="MB022_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBT4467040.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="MB022_002055"
     CDS             9425..11104
                     /locus_tag="MB022_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4467041.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALREYLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="MB022_002056"
     CDS             complement(11358..11558)
                     /locus_tag="MB022_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4467042.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="MB022_002057"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="MB022_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3592 a         2654 c         2131 g         3934 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat atttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta a
//
LOCUS       DAGCXG010000078        12209 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGCXG010000078 DAGCXG010000000
VERSION     DAGCXG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.081"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            419..751
                     /locus_tag="MB022_002058"
     CDS             419..751
                     /locus_tag="MB022_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467043.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            661..864
                     /locus_tag="MB022_002059"
     CDS             661..864
                     /locus_tag="MB022_002059"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4467044.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1048..1203
                     /locus_tag="MB022_002060"
     CDS             1048..1203
                     /locus_tag="MB022_002060"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4467045.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(1519..1926)
                     /locus_tag="MB022_002061"
     CDS             complement(1519..1926)
                     /locus_tag="MB022_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4467046.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(1921..3125)
                     /locus_tag="MB022_002062"
                     /pseudo
     CDS             complement(1921..3125)
                     /locus_tag="MB022_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            3251..3469
                     /locus_tag="MB022_002063"
     CDS             3251..3469
                     /locus_tag="MB022_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467047.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            3490..4125
                     /locus_tag="MB022_002064"
     CDS             3490..4125
                     /locus_tag="MB022_002064"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4467048.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4318..4557)
                     /locus_tag="MB022_002065"
     CDS             complement(4318..4557)
                     /locus_tag="MB022_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467049.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4581..6104)
                     /locus_tag="MB022_002066"
     CDS             complement(4581..6104)
                     /locus_tag="MB022_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4467050.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(6122..6244)
                     /locus_tag="MB022_002067"
     CDS             complement(6122..6244)
                     /locus_tag="MB022_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4467051.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(6274..7257)
                     /locus_tag="MB022_002068"
     CDS             complement(6274..7257)
                     /locus_tag="MB022_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4467052.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(7281..7430)
                     /gene="rpmG"
                     /locus_tag="MB022_002069"
     CDS             complement(7281..7430)
                     /gene="rpmG"
                     /locus_tag="MB022_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4467053.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7451..7828)
                     /locus_tag="MB022_002070"
     CDS             complement(7451..7828)
                     /locus_tag="MB022_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467054.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7860..8039)
                     /gene="rpmF"
                     /locus_tag="MB022_002071"
     CDS             complement(7860..8039)
                     /gene="rpmF"
                     /locus_tag="MB022_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4467055.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(8054..8323)
                     /gene="rpsN"
                     /locus_tag="MB022_002072"
     CDS             complement(8054..8323)
                     /gene="rpsN"
                     /locus_tag="MB022_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4467056.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8846..10612)
                     /locus_tag="MB022_002073"
     CDS             complement(8846..10612)
                     /locus_tag="MB022_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467057.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10572..>12209)
                     /locus_tag="MB022_002074"
     CDS             complement(10572..>12209)
                     /locus_tag="MB022_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467058.1"
                     /translation="YKFLKSLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHIL
                     GFRLETNLRKKGISGLTEASFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAI
                     VTPVLSVALGFFINFKIGLVLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVE
                     YIRGIQVIKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILI
                     VPITFFITSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLY
                     LEMKQDQLKYGNETQIKNCDIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSG
                     KSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSI
                     ANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKD
                     AKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKI
                     VERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT         3868 a         2329 c         1817 g         4195 t
ORIGIN      
        1 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttattaacgt
       61 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
      121 ttatttacgt acgaagtggg caggaggtcg aatccagaac tgggccatgg tacttcaacg
      181 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
      241 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
      301 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
      361 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
      421 gaaaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
      481 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
      541 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
      601 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
      661 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
      721 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
      781 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
      841 gtaaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgacg
      901 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
      961 gcaactcgta ttctacgtta ctatatggtg gattcacaca tgaaaaaaga gattgtcgtc
     1021 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     1081 cagcatcggt cttttattta tcaagagctg ttgaccgacc accacatcac acattcaatg
     1141 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     1201 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     1261 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     1321 tgacaatttg aagcgaagaa aataagtaat ctaaccgact gttaactaaa ccattgcagg
     1381 tcccgttaaa gatacttgtg cctgttgtca ccgcttcttt ttcttgtcca caagcgcctt
     1441 aacacactta ataaagcccc tgctattatt aataataata tctttttctt attaaaaacc
     1501 tcctcatcta tttccagatt agtaaatgta gtaatctttg ctttcttttt tctcaatact
     1561 aataactaac ttatggggta aacaaatact cgtttgaccg ggttttgaga tccatccagt
     1621 tctaaccgcg atttgatcag gactattatc ttctttaact cgaattcttc catctttaat
     1681 ctcaacaata ttatattgtc ccttcgcagg atgataattc acgagtttat gattgatctt
     1741 atccaaatcg aagcgatcca tttccttgcc atcaatttga acgatggcat atttgatagc
     1801 atcttcagtt gtttcattct ccttttctat agagaataca aataagggaa taaaagaggg
     1861 aataatcaaa atgccaataa ttatgacatc gcctatcttg acaccagctt ttcttaaaaa
     1921 tttcattaac gatatctggc taaacatatt agaatttttc catggaaatg gtgaaatttt
     1981 tcggaaatta tctgcattaa gagaatcagt taaacaaccc tcaagttgtt ccttaataca
     2041 ttgttgatcc atcttcatac caataaaaac tagtttatta atgcgttcac caaacaacgg
     2101 atgccaggct tccttcaatt cgggtttttt tgtataatca tttgacactc aattttaggt
     2161 aaactagcta cccaataatt taatgattca atacttttat ggctacccga tgtttcaaag
     2221 atcgtcgaaa aatcactatg ttgggcaaac caaagaaacc cttttgctct tgtaatattg
     2281 ccaggaaaat ttttatccgt aagatgggca aatttttgtg cgtcaaggag taaccgattt
     2341 cgatagataa aacttgagat accatattct tctgtttcag gaatatgatt ttgatagcca
     2401 agctctaact ctttgaacca gcccattgaa taggaagtct catctatatt aaaaagattt
     2461 gtatttagaa ttttcgataa gtctacttca cctttaattg tcctaattat tttggcattg
     2521 gactgtaact tagcaattag accgttaatt ttgtaaatcg tcttggttga cactaagtca
     2581 ctattattta aaagtaatat gttacaacac tcgaattgat caattaacaa gccacgaata
     2641 tcctgttctt cttcaactac attaccttca gcttttcttt cctgaatctt ttcacccgtt
     2701 tcgaaattat cccaaaaacg ttttacatcc actactataa taatggcacc aatctctaat
     2761 cgttgggtca aatcaatgtc taaagggttt ttagctaata taattgcttg aataattgga
     2821 attggtttgc tgattcctga agcttcaata acaatttgat tcaagtcaga aaattgcact
     2881 aattgatcta gttcaacaat caaatcttcg cgtaatgtac aacaaatgca tccattcggc
     2941 atcgaaacaa gtttttctcc agttctttta aaacctttct tgtcgactaa taccccatca
     3001 acatttactt cccctatgtc atttacaatg acagcaattt cctttccgtg atcaccgtac
     3061 agaatatgat ttagaagtat cgtttttcct gaaccaagat agccagttaa gattgtaata
     3121 ggtatttttt catagggaac ctctttctat accttgaatt tacactttaa gtgcaacaca
     3181 taaaaatgag tcataatcga tatccaacta gtataagttt gtcgagaaca ctcctagaag
     3241 gaaaataatt atgagccagt attgtcatct tactatactc aaacgagaaa gaatctttct
     3301 gcttcacgaa gaaggatttt ctattagtat aattggcaac gacattcagc atagcccgtt
     3361 tactatttta catgaactga gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa
     3421 aaaaattatg cttatcgaag aaaaaaatgc ggacgttatc acattctaaa tgatccgtga
     3481 gtttggtcaa ttgttcgtca tttatttgtc gattttcagt ggtcgccaga gaaaatctcg
     3541 aatcgtttga agcatgaaca tatggacttt tcaattagtt ttatcacgat ctaccgtgca
     3601 atttgcaatg atcttttcga tacagaaccc ctctcacacg gcaataaagg cctgatccgt
     3661 tcattgcacc atcgaggaaa aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag
     3721 atttgcctta ctcattcgat ccatggccat ccaaaatccg cagatgatag aagtgatttt
     3781 ggtcattgga aagccgatat tgtagctggc aaaactagtg gtacttgttt agtgactttg
     3841 acagacagaa aatcatgctt ccttttagca ggaaagaagg tccccagaaa gaaagccgct
     3901 tatgtgagcg agaaaatgat tgaactttta agcagtattc caaatgaggc ggtcaaaacg
     3961 atcactccag atagaggaaa agaattctcc aatcatttcg cagttacaga agtattgaat
     4021 aatatgccct tttattttcc agatcctcat gcccttggca acaatgaaca aattaaaata
     4081 cgaatagact cattcgagaa tacttgccaa aatcgaaaga gatagctacg gtagataact
     4141 caacgataaa tcagatgatt gaaaaactga ataacagacc tagaaagtgt ttagattgga
     4201 gaacacctta tgaagtcttc tttgataaag tgttgcgctt gatttgacaa ttcaagaaat
     4261 aataaaaagc agatcaaatc caaataaggg aaaaatgatc tactctggta ttatcaatta
     4321 attggccaag ctattatgcc gttttgctaa acgtctactt cttccagtat tgaccttgat
     4381 aaaacaacaa tcaggatccc gttttaatac tctatgaact gtgtcaatac taacatgtaa
     4441 agttgcagca atatcagcta atgtactccc atttaatctc atttgctttg caaattgata
     4501 acgccgataa gcatcaatat tcttggaatc catagacaat aatacttttc ttaccatgta
     4561 tttcctccaa agattggatt ttaatgggcc atcatttctt gagcaatctc atgctcagat
     4621 aatgaagctg gatagaaagt aggccaatta tgcatttcat tataaagttt ttcttgactt
     4681 tctccaccaa agaaaatatg gaaatgctct gccttacttg gtgcaattcc atgatcgcta
     4741 aattgaatat atttaaaacg accagcacct gcatcatcag tttcaaaatt aaaacgaaca
     4801 ccacggtttc ctttttcata attcaaaatt ttgtatccaa catatttgta tgtgtattga
     4861 tgatgttcac cgttgactac aaaatcaatg gtgttgtctt taatgttgat agtatcaata
     4921 tctgtcttat agccagtagt ataataatct ttgtactcgg cagctgtcat gtctttattg
     4981 agttttgctt tgtaatcgaa aacttcatcc aaaattccat cttttaacaa cggataaaca
     5041 gattgccatt cgcctgtata atcactcaat tcgcgatcct tcacatcggc atctttaaag
     5101 tacccattag caactgtctt ttcatccttc gcttcttttt ctggaactac ctcttcacca
     5161 gcaaccatag ttgttttctc taacgctttt agattgtctt ccattactga aacataatct
     5221 tcaccattat ccatttgttc ttttgtaaga ctttcaagag gattcaatac ttccaatttt
     5281 atcccagctt cattggccaa agtttttgcg attttatcat tagcattctt ttcaaagtaa
     5341 atataattga tcttattttt tttaacatac tcttttaatt cagctaatcg accagaagat
     5401 ggctcttctt caggacttag tccagcaata ggaacttgta tcaatccata atccaaagct
     5461 aaatatccaa aggctgcatg ttgtgtcaca aaactttttt gtttcgcttc tttaagacta
     5521 gtggtatagt cagcatccaa ccgtttcagt ttagtcaaat acttttcagc atttgtctca
     5581 aatacttttg cttttttcgg atacaattta actaactgat ccttgatatt tgaaacttcc
     5641 ttaattgcca ttttgggtga aacccacgta tgaggatcta gttcatgatg gtgatcttct
     5701 tctccgtgat catgatcttc ttcttcacta cctggcaata aaatcatgtc ttttgttcct
     5761 tctactacat taggcccagc tttcttccaa ctttctttag cttttggtac ccatgattcc
     5821 atgttttcgt tatgatatac aaaaacatca gcatcatgag cttttgccat atccttagct
     5881 gagggctcat agtcatgagg ttctgatccc gctggaacca ttaaatccac gttcgcttca
     5941 tcgccgacga tgtttttggt gaaatcatac attggataga atgttgttac aatttctaat
     6001 tttccatctt tatcattcga tacttttgca tcattagtgc tggagcaagc tcccaataat
     6061 cctagagctg ctatcccaat aagtcctaca ctgattttct tcattctttt tccttcctta
     6121 attatgctaa tttcttcatt ttattttgtt tatgttcttt gataattttt aatgcccttt
     6181 ttctagtttt gatattcggg cttttaagtc gtcgatatgc actagatata tccgttttct
     6241 ccattagaaa ttacccctaa tcaatactta tcattatttg ctttccgcca gtaatttatc
     6301 aaaactctgt ttgatccttt cttcatccaa catttttcca attagaataa tagtggtttg
     6361 gtctttttgt cgagtggtgt attgaaaatt aacctgttcg tttacccctt gtatggcaac
     6421 tataaaatct tgatcttgca aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt
     6481 ggttaaaatc agccggtcaa ttaattggaa taaaaaatct ttttttatgt cttgatctga
     6541 agttaagaga attgatttaa actcatgttc ttgatgatgt tgatgatagt gtttgccatt
     6601 atagtgattt tctgatgaat cactttcaaa aactaaggga tgattgaatt tttccaaatt
     6661 ataaaaatcc tttttttgaa tcgtttcact atgtgtgaac attcgaatat cggataaagg
     6721 gttaatactt ttgatttctc tctgtaattt ttttagtgca gataaattca agttttgttt
     6781 tctagacaca aaaatgcgat ctgctatgac aagttgctta agggcttgtg gttctctttc
     6841 aataagttca aaactgtcac tatcaataac agtcaataaa ctatcgatat atactcttcc
     6901 tcttagttga ggcgctgtta aaagtgtttg aatgattggt tggggatctg ctatacctgt
     6961 agtttcaatg atgatttgtg aaattggttg ttgatgttct tctattgttt ccagtaaaga
     7021 aatcaatatt gctaataaat cactgcgaag actgcaacac atacatccat tatttagttg
     7081 taacacccgt tcctcgctat gaactaataa tttgtgatca atcccaactt caccaaactc
     7141 attttcaatt ataataattt cttccctggg gtagggtgag acttgcaagg cttgattgat
     7201 tagcgttgtt tttccagccc ctaaaaaccc agaaacgatt gtaactggta tgcccatttt
     7261 gtcacctttt taccacttag ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt
     7321 ggcgaatact tttttaattc taaccgttca gggttattcc gtttattttt gctggtgaga
     7381 tacagacgtt ctccagtttc gacacattcc aaaataatat tttgacgcat aaatcttcct
     7441 ccttcaatac ttatcgatct tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta
     7501 actcgccaga ttccaattgt gaaactaaat tttctttcat atcaacaggt atctcccaat
     7561 aaccatcaat acattgaaat aactcctcgt catactcttc aaaaattgga tagattctta
     7621 aaaaatcatc aaatgataag ccttggtcct catagtaatc tttgactaat ccattttcaa
     7681 ttgcctgttg ggtcggtaaa cccgtttcag taataattcc tctctctacc atcgaacgat
     7741 aaaaagtgca tttatctagc gattgctggg tcgtttcaag ataatacttt aatccttctt
     7801 caactaaagg tgggtaaata atttccattt tgattccctc ctaaaagtta tatattaaac
     7861 tattttgaag ctcctttaat tactttcttt ccattatagt acccttttaa agatacatga
     7921 tggctacgtc tataatcacc attcgcttcg tcaaatgaaa tttctggttt tactacagtt
     7981 tgatgagttc ttcttaaccg tttcttcgcc ttagatgttt ttcttgctgg tactgccatg
     8041 atatttcctc cttttaccaa cttgcttttt ttactccagg aatcaaccct tgatgggcaa
     8101 gttctctaaa cttaatacgg gacataccga actttcgcat ataccctctt ggacgaccat
     8161 ccgtctgatc acgaagcctc agacgattcg gatttgaatc tcttggcaat ttagcgagag
     8221 cggtgcgatc tcctgcggct tttaattcct gacgtttatc tgcgtacttt tcaatcatcg
     8281 ccatttgttt ctttgcttta gcaattttcg acttttttgc catatacctt tatactcctt
     8341 ttattttgat ttagacatct caaatcgtaa tcgttacgtt ttgacactga tactttacca
     8401 acaagagttg ctcttgtcaa caataaataa catgaaataa aactcatatg aatagataac
     8461 tatttgaaaa cgaatttatc ggtacataga gataatcaaa tcatttaaaa gaatatgcag
     8521 tttaagctct gaaaaataag aattcacttt tattagtcca atcaaacaca aataccgttt
     8581 taagtagctt ttcctctact taaaacggca atatgaaaga aactataact ttttattcaa
     8641 tcttttatgt ttaactggtt tatcgccaat tttttaaaac tttgattatt ccttatatta
     8701 taagaataga cattaagtga actaattcaa tcatactgat tctttcgtgt attcaaaatt
     8761 agatgctgtt taataaaatt attctttttg aaaaagaatt ataactatat cccaaaaatt
     8821 gtaggttaat ttaaagccta tttcatcatc gtgattgagc ttctttttca tagacgaatt
     8881 tctcagatag tttagttttc tttatgagat ttttgtatat ttctgacctt cgatacaact
     8941 cactatgagt accttgatca actatttttc cttgatccat cactacaatt ttatttacct
     9001 tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt attaaacgat
     9061 ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta atttcatcta
     9121 aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc
     9181 ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca ggtagttttt
     9241 caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca tctgacgctt
     9301 gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa
     9361 aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata tcatacccat
     9421 caattaaaat acagccttca tcataatcat acagtctaga cactaatttc aagatactag
     9481 ttttcccgga accactagca cctactaacg cagtcacttc tccttgttta gccgtaaaag
     9541 agatatgatc taaaattggg gtattgttat catatgagaa agagacatct cgcaactcaa
     9601 tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc ttcatagctc
     9661 taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct ttcatagaat
     9721 cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag agtatagtca
     9781 cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa ctaacatatg
     9841 agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt aaatgaatcc
     9901 tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat agattaaagc
     9961 tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttt tctctcaaag
    10021 tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag attaagacaa
    10081 aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca accaaaagca
    10141 tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt gcatgactca
    10201 tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct gataaattat
    10261 gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat
    10321 gcgcactttc tttataagta ctattatata agttttcata ctccctatct aacaaccaat
    10381 acatgaaaag caaagtaacg aacgatactg taaaataaag ccaaggcggc tttacatttt
    10441 ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata ttaatgcagt
    10501 atacaaaaaa gctagaaata gatgctttaa ccaaatcttc tgcgccctga ttagttaaag
    10561 cgaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc attctcataa
    10621 ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg atttccacgt
    10681 tctactattt tcccttctga caagacaaga atttcatcta cagatttaat gctggataat
    10741 cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc cttttgtagt
    10801 tcatactcat tatctggatc aatagatgca ctggcttcat ccataataac aatctttgca
    10861 tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga cagataaact
    10921 cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat cgattcgcaa
    10981 ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt tgctatagaa
    11041 acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa agcaattgtg
    11101 ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt aatctgtccc
    11161 ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc gcttcctgat
    11221 tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt ttctaaaaca
    11281 cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt
    11341 gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga gtctagtgtt
    11401 tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct catgaaagaa
    11461 acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa tatcttggga
    11521 gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac tatgccaaaa
    11581 aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt atatgcaaac
    11641 ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt agcaccaaat
    11701 atctttataa cttgaattcc tcggatatat tctaccgttt cagagcttaa cacatctaac
    11761 gattcttgat atttttgaat aaaatttgta ttctctgtca ttttctttaa caagaataca
    11821 ccaattacta acatggcgat tagaaccaat ccaattttaa aattaatgaa aaagcctaag
    11881 gcaactgata aaacaggtgt tactatggct tgagcactat ctggaatcaa gtgagcaatt
    11941 gccatatgtg ttttagccgc gttgtcatca attgtctttc gaatatatcc cgaagaattt
    12001 aaatcaaaaa aacgaaaact tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta
    12061 gtctctaagc gaaacccaag gatatgagaa aataagccag aaaaaatata aaataaagct
    12121 cctaaagtta accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct
    12181 acaataatta gagacttaag aaatttgta
//
LOCUS       DAGCXG010000079        11978 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGCXG010000079 DAGCXG010000000
VERSION     DAGCXG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.082"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            53..295
                     /gene="yaaA"
                     /locus_tag="MB022_002075"
     CDS             53..295
                     /gene="yaaA"
                     /locus_tag="MB022_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBT4467059.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            282..1406
                     /gene="recF"
                     /locus_tag="MB022_002076"
     CDS             282..1406
                     /gene="recF"
                     /locus_tag="MB022_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBT4467060.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1403..3349
                     /gene="gyrB"
                     /locus_tag="MB022_002077"
     CDS             1403..3349
                     /gene="gyrB"
                     /locus_tag="MB022_002077"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBT4467061.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3369..5840
                     /gene="gyrA"
                     /locus_tag="MB022_002078"
     CDS             3369..5840
                     /gene="gyrA"
                     /locus_tag="MB022_002078"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBT4467062.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6076..6375
                     /locus_tag="MB022_002079"
     CDS             6076..6375
                     /locus_tag="MB022_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBT4467063.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6425..6937
                     /gene="ssb"
                     /locus_tag="MB022_002080"
     CDS             6425..6937
                     /gene="ssb"
                     /locus_tag="MB022_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4467064.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6963..7199
                     /locus_tag="MB022_002081"
     CDS             6963..7199
                     /locus_tag="MB022_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBT4467065.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7342..9315
                     /locus_tag="MB022_002082"
     CDS             7342..9315
                     /locus_tag="MB022_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBT4467066.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9321..9773
                     /gene="rplI"
                     /locus_tag="MB022_002083"
     CDS             9321..9773
                     /gene="rplI"
                     /locus_tag="MB022_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBT4467067.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10047..11414
                     /gene="dnaB"
                     /locus_tag="MB022_002084"
     CDS             10047..11414
                     /gene="dnaB"
                     /locus_tag="MB022_002084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBT4467068.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4007 a         2179 c         2651 g         3141 t
ORIGIN      
        1 aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg cattgaaaca
       61 acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag aagtcacagt
      121 tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt tagtcgatgg
      181 cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg aagttccaga
      241 agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg aataagctgt
      301 atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag aacctcgtca
      361 tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc tacgtattag
      421 ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg actgacgatc
      481 aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta gaaatcttat
      541 tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag ttaagtactt
      601 acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta gtaaaaggca
      661 gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat ccaatctatc
      721 tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac ttgaagcaat
      781 tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa caactagcag
      841 cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg gagttttggg
      901 ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc gagtatctca
      961 ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa tttctcgcac
     1021 ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt ggtcctcatc
     1081 gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga tcgcaaggac
     1141 agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt aaagaagaga
     1201 caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat gataatcgtc
     1261 aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca acaacgacac
     1321 ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt caacaaggga
     1381 aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac gcgctaaaga
     1441 gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta aacgacctgg
     1501 catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg aaattgtcga
     1561 taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga ttattgaaaa
     1621 agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata tccaggcaaa
     1681 aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag gaaaattcgg
     1741 cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg ttgtaaacgc
     1801 gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact atcaagaata
     1861 tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc gccatggaac
     1921 aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt ttgatttttc
     1981 aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc gtatatcgat
     2041 tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg gcgggatcaa
     2101 aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac ctgtatattt
     2161 ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag acggctacca
     2221 ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa cacatgaatc
     2281 aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac aaaagctgat
     2341 gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga cagctgttat
     2401 ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat tgggcaattc
     2461 cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat tcttgctgga
     2521 aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca gagctcgtct
     2581 agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga ttagtaatct
     2641 gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat tgttcatcgt
     2701 cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt ttcaagccat
     2761 cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca agatcttggc
     2821 aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag aagattttga
     2881 tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg tcgacggcgc
     2941 gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta ttgttgaagc
     3001 tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga agaatattac
     3061 gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg cttcacttcc
     3121 tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg atgatcatca
     3181 actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca gtgtggacga
     3241 tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag aaccccgtcg
     3301 tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat catgaaggag
     3361 gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta accagcgaaa
     3421 tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca cttcctgatg
     3481 tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac gaattaggtg
     3541 taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc atgggtaaat
     3601 accatcctca tggagacaga gcgatctatg agtcaatggt acggatggca cagccattca
     3661 gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac ggtgacggtg
     3721 ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa atgctgcgtg
     3781 atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa aaagaaccag
     3841 ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg atcgccgtag
     3901 gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct atcgatttgc
     3961 tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct ggaccagatt
     4021 ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat gaaacaggta
     4081 agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat ggtaaagaac
     4141 ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc gaacggattt
     4201 ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac gaatcttccc
     4261 gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc gttatcttga
     4321 ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg ttggcgatcg
     4381 aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat atcgaacatc
     4441 aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa gcaagagcac
     4501 acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt gcgattatcc
     4561 gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc gatttatccg
     4621 atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt ttggaacggg
     4681 aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta actgacattc
     4741 tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac ttgaatcaac
     4801 gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt ctggaagatg
     4861 aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt tacatcaaac
     4921 gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc caaggaatgg
     4981 gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat gatacattgt
     5041 tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt ccagaatacg
     5101 ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg agtgaaagca
     5161 tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc ttctttacga
     5221 cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc cgaagtaatg
     5281 gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta ctgacagatg
     5341 gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc aaagaagaag
     5401 cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg cgtgaggaag
     5461 attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg atcacagaaa
     5521 aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc ggaggtaaag
     5581 ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg accactgttt
     5641 ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt ttcaacgtat
     5701 cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag atggaagctg
     5761 atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa acagttgaag
     5821 aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag aataaccgat
     5881 taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct taaaatattc
     5941 gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag agcttgtgag
     6001 tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg aaagtccaca
     6061 gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat tcgtccaaac
     6121 attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt gaaagataac
     6181 ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta tgaaatgaac
     6241 ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc agcaaatgct
     6301 gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca catgattgtt
     6361 aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag aggagatgac
     6421 tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag atttgcgtta
     6481 cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta attttacaaa
     6541 ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca aatcagcaga
     6601 aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac gtatccaaac
     6661 tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag tagctgacaa
     6721 cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg gcggattcca
     6781 aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc aaacatctca
     6841 atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg gctcaagttc
     6901 aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag gagggaaata
     6961 gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac attgctgcta
     7021 accatattga atatatcgat tataaagata tcgaattact aaaaagattt atttcagaac
     7081 gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa cgtaaaatta
     7141 ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc gaagactaat
     7201 aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct ttttttctgt
     7261 aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa aatcaaatta
     7321 gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc gaaacctctc
     7381 ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc atttcgtttt
     7441 gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg gttgaaaatg
     7501 aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac tgtagctgaa
     7561 accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta tcagccatca
     7621 agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca aagtggatta
     7681 acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa aaaccaagtc
     7741 tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt tgtttatttt
     7801 aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca gccggtgatt
     7861 ggtattttat ctattgataa ctatgcagat gccattgata aattagatga caaagagatt
     7921 tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga ataccatgtc
     7981 tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat cgatctgaaa
     8041 aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc tgaaaaacag
     8101 agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct cgatgaaatc
     8161 ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga ccaagttgtc
     8221 gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc tgctaagaat
     8281 gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg gatttttgat
     8341 gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc tttaggctct
     8401 gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat cgttatcaat
     8461 gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa gcatcctgaa
     8521 ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga agaggacctc
     8581 ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct atatgagcaa
     8641 tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc aagtaaacct
     8701 ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc tgagctgatc
     8761 caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt attgcttgca
     8821 gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac ttttgacgtt
     8881 gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt attgagctcg
     8941 gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta tgtgacttct
     9001 gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat agcagcaaaa
     9061 acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat tacaaagcgg
     9121 acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt ccaaatcatc
     9181 atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact ggaaaatacg
     9241 acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat gaatcaaata
     9301 tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa ggaaaaggta
     9361 aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta atcaaaaata
     9421 acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa aagaaagcac
     9481 aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa ttcttggaaa
     9541 aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt ttgtttggtt
     9601 cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc aaattagata
     9661 aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac gtacctgtta
     9721 agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa taacaaatct
     9781 tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac cgaaatttat
     9841 ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag attgatttcg
     9901 gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa aagagaacta
     9961 tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt gtaaaatgaa
    10021 gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga atcccaccgc
    10081 aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca gaaacgatca
    10141 ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat caaattattt
    10201 ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt actgtcaaag
    10261 accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat ctctcagact
    10321 tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc gttgaagaaa
    10381 aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa ggattcgagc
    10441 aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg gaggtttcgg
    10501 aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc acgattgaaa
    10561 atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact ggctatcaag
    10621 gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt gctgcacgtc
    10681 cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga acaaaaacag
    10741 atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta aatcggatgc
    10801 tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt tcggaagaag
    10861 aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt tttatcgatg
    10921 atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta gcgcaagaaa
    10981 aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt aacggtaaag
    11041 agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg gcgaaagagc
    11101 tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag cgacaagaca
    11161 aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat gcggacatcg
    11221 tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac gatgatggaa
    11281 acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga agcggtgcac
    11341 gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg ctttcgccaa
    11401 gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct ctcgtaagaa
    11461 gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat ctccaaaggg
    11521 attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc aatcagctga
    11581 cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg tttgtttctc
    11641 taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt gttctcctgc
    11701 caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt ttgaaattgc
    11761 tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg gtgttcaaaa
    11821 gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta tttttgtttg
    11881 gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca cggtgttcca
    11941 aaagtagctt cttcgaaaaa taagtcgaaa tttcacaa
//
LOCUS       DAGCXG010000080        11823 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGCXG010000080 DAGCXG010000000
VERSION     DAGCXG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.083"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(148..525)
                     /locus_tag="MB022_002085"
     CDS             complement(148..525)
                     /locus_tag="MB022_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467069.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(825..1706)
                     /gene="galU"
                     /locus_tag="MB022_002086"
     CDS             complement(825..1706)
                     /gene="galU"
                     /locus_tag="MB022_002086"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4467070.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1749..2768)
                     /locus_tag="MB022_002087"
     CDS             complement(1749..2768)
                     /locus_tag="MB022_002087"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4467071.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3401..4405)
                     /locus_tag="MB022_002088"
     CDS             complement(3401..4405)
                     /locus_tag="MB022_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4467072.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4418..5467)
                     /locus_tag="MB022_002089"
     CDS             complement(4418..5467)
                     /locus_tag="MB022_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4467073.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5484..6305)
                     /locus_tag="MB022_002090"
     CDS             complement(5484..6305)
                     /locus_tag="MB022_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4467074.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6292..7071)
                     /locus_tag="MB022_002091"
     CDS             complement(6292..7071)
                     /locus_tag="MB022_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4467075.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7088..7558)
                     /locus_tag="MB022_002092"
     CDS             complement(7088..7558)
                     /locus_tag="MB022_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4467076.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7570..7989)
                     /locus_tag="MB022_002093"
     CDS             complement(7570..7989)
                     /locus_tag="MB022_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBT4467077.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8070..10847)
                     /locus_tag="MB022_002094"
     CDS             complement(8070..10847)
                     /locus_tag="MB022_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4467078.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11038..11262
                     /locus_tag="MB022_002095"
     CDS             11038..11262
                     /locus_tag="MB022_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467079.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3431 a         2486 c         1912 g         3994 t
ORIGIN      
        1 gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa
       61 aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta
      121 tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tactttgttc
      181 gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta tccgagactt
      241 tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata aagcaaatac
      301 gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc caacggatgc
      361 tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa acatgataaa
      421 tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca tcagccactt
      481 gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga acacacacct
      541 ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc
      601 atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat
      661 aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac
      721 tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt
      781 aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt
      841 agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga
      901 agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt
      961 cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt
     1021 agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga
     1081 gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt
     1141 tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt
     1201 ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt
     1261 tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc
     1321 cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc
     1381 cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca
     1441 actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt
     1501 gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg
     1561 attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga
     1621 agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca
     1681 ggaatgactg cttttttgac cttcacttgc tgttcctcct aaacgacgat taactattga
     1741 tcatctattt aacgaagaga aaattcattc tccatttttc cgtcacgtaa catgatttct
     1801 tttgctgctt ttttgatatc ttctccatta tacaaaacat tatagattgt ttgagtaatt
     1861 ggcatctcaa cacctagctg ttgagctagc tctactgctg cttttgtcgt tgccacaccc
     1921 tcaacgacca ttcccatgtt ttctagaact tcttccagct tatgtccctg tcctaacaag
     1981 ttaccggcac gccaattgcg agaatgaaca cttgtacaag taacgatcaa gtcccccaca
     2041 ccactcaaac cgataaatgt cagaggattg gcacccatcg cgacaccaag acggctgatt
     2101 tctgccaatc ctcgagtcat gattgctgct tttgcatcat ctccgaatcc taatccatgg
     2161 atagcgccgg caccaattgc aatgatattt ttcagtgctg caccagtttc tacgccaatc
     2221 acatcattat ttgtataaat cctgaaataa tcattcatga acagtttttg tacataggct
     2281 gctgcttttt gatcaatact tgcagctgta attgttgtga tatcatgtac tgccacttcc
     2341 tcagcatggc taggaccaga taatacaaca attgcctgtc ttttttcttc tggaatttct
     2401 tcagccaaaa cttctgagat tcgtttatgc gtatcttgtt ctagtccttt acttgcatgt
     2461 ataatgattg gcttagtatt cattttacca ataagttcct gtgcgactga acgaatcgct
     2521 tttgttggaa caacaaataa aatagcatct gcgttatcca ctgcttctgc caattcttta
     2581 tatccgataa tcgattctgg gatcgtcaaa tcaggtaaaa aatgctgatt tgtatgatac
     2641 ttattgattt catcaatctg ttcaggtaca tttccccaaa tacggacatc atgcccgttt
     2701 tctgccaatg tttgtgctaa agcagtgccc caagatccag gaccaagaac tgcgattttt
     2761 tgtttcatgg atattcctcc aaataaataa ttgataattt ccattatagt atacatttct
     2821 taaagttggg actaatttcc attgttttta agtgtttgtt tacttctttt atcgttttta
     2881 cttgaaataa tcaatgatat gttacaatag tctacatttt atttttacac tcgttatgct
     2941 ttgcttgttc ctaagaggaa gcagttttga caatgttact accagcatta ctattaggag
     3001 gttaggaaac ttctcctagt gagtattgat ttaaaataca taagagacag attgaacatt
     3061 tctaaatctg tcaggtcctg caacggacag attgtctctg ttgacggttg acataggaac
     3121 ttttgtttta gtcaacattc tggatacaaa acgctatgta aactggcgca actcgttgta
     3181 cttggacttt tactacggat ccatgcaaca actaaatagt actattgcat aacaaaaaat
     3241 ggttggcacc cacatgtccc attaaacaga ttataagttg aactgtagat aatgcaaaaa
     3301 atgtgtttta taacgtaatt gatgccaata ttttaaaaaa ataaagggag cagttatcgc
     3361 tgctctcttt attttttata ttctttttat ccagtctatc ctaataatca aactgacgat
     3421 agtagcggcg tatctctaat ggatggcagt tcaactgctc cacatgagca tcgatgcgtt
     3481 gtgtcactac atgcatcaat aagaaagaca aaacacgtcc gcgatatttt tcactgatac
     3541 cttctactgg atagtctttt gtgtctatca atgtataatt gctgcaaatt tttggcaaaa
     3601 gtttagccac acgttcagat aacactctct gttcatcttc gcctaaaaat aatgtgactg
     3661 gggttgtttc ttctacgatc tctaacgtcc cgtgaagaaa ctcggcagca tggatagatt
     3721 tcgatggaag ccagctttgt tcttcccagt aacacatggc atatgaataa acagctcccc
     3781 attgattccc tgctccaata aaataatgca tactatcacg tcgatgtttt tctgcaaatg
     3841 ataagccaaa tgcatctgct tttttctctg cttccactaa ccctgttggt aaataacgtt
     3901 cgagctgttc atagtattct gaataagctt cgaattctcc attcaaatac atcaatcgat
     3961 ctgctgccat aaaaaatttg atttgttctg ttccaggagc tggataagtg accacaaaat
     4021 cgcatagctg agacaactta ctgttcgctt tatcgataaa tccaatcagt gtcgctccta
     4081 ctttcttgat ttgttctact gctttcacta cttcttgagt tgtaccggta acagaggaca
     4141 aaataacgat agaatctttt gttaatcttt tgtttccagt agtgtaatat tctgcagcat
     4201 gctgtacaaa gacaggcaag ttgcttttcc cattcatata agtgacagct tgcatcgagg
     4261 aagcatacgt tccaccgatt cctagataat agacagcgtc aaatttttcc gcataaagtt
     4321 tatcgatgat ttcttctact tgcggacgta aagctagtgc gccttttatt gctttgattt
     4381 gttcttcttc attgaatttc agcatgattt ccacctctta taatttactt ttcaagactg
     4441 tatcaaagtc ttcaaaaatc tttttgttca agtcaatacc tttagctact gcggttttat
     4501 aagcgaatag ttgaaacgga acgacaagcg ctaatacaga cagatattca tcacattcta
     4561 ttgctaatcc aagtgttttc tcgtttcttg ctttttttgt ggtaatggtg agagtctctc
     4621 caacgtaccg actcatatat ctttgcaact tttgcgaacg ttccttattt acactttccc
     4681 cttcaataaa gaatagcaaa tgcgaagcat ctgcctccag gtatgggccg tgcatataag
     4741 cttctaactc aaatccttgt gacggaacgc ggattgtttc agtcaatttc gtctctgctt
     4801 ctttagctgt tccccagttt ggaccatatc caatggtgac gaaacgctgt gctaaacgaa
     4861 ataaactttg atatccatca aaaaattctt ccgttttttg aataatcgct ggtaaatggt
     4921 tgattatttt gcgcaactgt tgcatgtagt cttgttctat ttttttgctg attttatttt
     4981 tacttattcc aattcctatc cctaagagta gtaactgtaa aacagtcgca gaatagcctt
     5041 ttgtaacgaa gcctactgat tctaccccgg cacctaaatc aataatttgt gcagcttcta
     5101 aagcaagtgg actttgcacg tcatttgtga tagcaacaac aggtaaccct tgtttttgta
     5161 tcatttttac tgcttcaaca gtagaagcac ttttcccgct ttgtgacaca gcaatcacag
     5221 tatcgattga aggagaaagt tttccataat tgaaaaaata aaaaggttct tgaatatcga
     5281 cagtaagatc accataagat tccaatgctg gcttcgctgc taaacaagcg ttataagatg
     5341 aacccgttgc taagatcagt aaattcactg tttttttcat atgttctctt gtcgaaaaat
     5401 cattcttttt caagatcgta cttagtactt ccttttcttc atagatataa tcttgcatcg
     5461 taaacatttt ctctgctcct tttttaaaaa atccctatcc acgaaccaaa aattccaaag
     5521 aaagctagtc cgatcaagat cgttaatggt ttcacttctt tcttcaatag ataaaacact
     5581 atgccaaatg ctccaagaca taagatattt ggaacgatat catcaaaaat actttgaaca
     5641 gtcacagcat tttctcctga accgattttt aaaggaatat tgatggtgat catcgtggca
     5701 gtcattgctc caacgaccat cagtccaata atagacgcgc ctaatgtcaa actttccatc
     5761 ataccgtttg cctggatctt tttcaaaaaa cccgttccta agcgatatcc ccaacctgtt
     5821 gctagatagc gaaacagaat gtgcggaata ttgaaaatca aaatgaacaa gatagggcct
     5881 aaaatatttc cttgcatggc taaagaggta cccacacctg tggcaatcaa tcgcaaagtt
     5941 ccccaaaaga aagaatctcc taaccctgca agaggtccca tcaaagatgt tttgatacta
     6001 tcgatcgcag tcacatcaaa ttcaggatca tccttatttt cttcctccat tgcagccgtg
     6061 attcctaaaa tcagtgtaac gatatgtggg gtcgtgttaa aaaactctag atgccgtttc
     6121 aaagctgctg ccatttcttc tttcgactga tataactttt tcaaaatcgg aatcattgca
     6181 taaacataag ccatattcat ctgacgttca taattccaag aaaactccat ttgaaaagaa
     6241 cgccaaaata cgtgatttag ctcttttttc gttattttgc tgtttttgat ctcagaattc
     6301 ttcatcttct tctacctccc ctgcagttgt ttgaagtggt agttgacgga cattcataat
     6361 attgactacc acaactgcta aaatcgcacc aaaaatagct actccggtca caggaatctc
     6421 taagtaagaa gctatagcaa aacctaagaa aaaatatggt gcaacttgtt tgttgatcag
     6481 caaacgagca agcatcgcaa aaccaagagc tggaatcaac cctgtagcta ctgacaaccc
     6541 cttttgaacg aagtcaggaa tcatatttaa tagatttccg atcgtattgc ttcccaccat
     6601 ataagaaacc attaccacaa caccaagcat caaggaaaga ccgataccgg caagcaagtg
     6661 catatgctcg actcctttat aatttccttc ttctgcataa ttgtctgctt tatggttcat
     6721 aattggaatc aaaagcccta agtaaatatt tttcaatatc aatgtcaacg tagcaatcgg
     6781 caaacctagt aaaagcgcgg tttctgtacc ggctccagct gtaatagcaa aagccgtccc
     6841 taaaatccca ccggtcacaa catcaggtgg aatcgaagca cctactgaga acgatccgat
     6901 aaatgctaac tctaaagttg cgcccataat gatcccagtt tttacatcac ctaacacgat
     6961 tcctgtaaac agccccgtca caatcggacg agaaagcaac gatgtcccta gtgcatattc
     7021 tgattgtgcg acaaatgcaa caattcctaa taagactgct tgtacaatca ttgaggttct
     7081 tcctttctta caaaacttct cttacatcca cttttttatc tgctggtact tgacggattt
     7141 ctacttctat ttgtttgtca attaatcgtt tcaagcgttc ctcctcttct ggtaaaatat
     7201 ttaccgcttt tccgatgctg cgagtaccag gttttgcctt gatccctcct aagttgatgg
     7261 ttttgatttc tggatacgct tctgccagct tatatgcatc ctcgatcgat tccactacga
     7321 tgaataactt atatttatct gtcaccccgc ttttcaacgc ggcaatcgag tcatcaattg
     7381 atttgatcac tagtttgact ccttgtggct tggctaattt gatcgttgtc tttcgtatct
     7441 cattcgtagg tacatcatca ttagcaatca aaatacagtc tgcgcctatt gtttgtgtcc
     7501 atgaaaaagc tacttgtccg tgtaataacc gatggtcgac tcttgtcaat acaatcattt
     7561 ttcttcctcc taaaattcgt cttcttctat tttctgattg agccgctcgt tacaaaactg
     7621 gatcattttt tttgaatcag ttagcacctg ctggatcaaa acagttgtct ctacttcagc
     7681 gtccaaccga gatactagct caatcaatag tggcaaattc aatccggcaa cgagatagac
     7741 atgatcccga ttgatatatt gtaagaattc gttgttgaca cttccgccaa aaagatccgt
     7801 cagaacaatg atttctttat ctttgtacct cataaaaagc tgccttactg tttccgctaa
     7861 atcaaaattc tcatcagtat agcaatcgaa tacagtaata gaatgatttt tcccatagat
     7921 cagctctaat gaatctaata tccctgaagc aaattttcca tgggaagcta agataatttc
     7981 tctatccact ttttcatctc ctcttttcac ttacacatac taaaaaagca agttgcatgc
     8041 caaaattagt ggcaacaact tgcttatgtt caaaattctt cttctatcga gagaaactct
     8101 gtatcttcac tcaaaatatc atagatatag gcaatctcag aattggggat catgacacta
     8161 taatgctctt caatgacact aaacgcagat ttaatagcag ataacccttc tttttgacac
     8221 tgcttccatt tctctaatcc ttgataggtt tcgattggca tttgtcggat caatcgttca
     8281 atgagacagc tcacatggac atacaacgct aattttttgg gattgctgat gcttcgattc
     8341 aactgcacct ctaattctct cataaaatga tcgatttctc ccatcacctt ctctgtatct
     8401 agaatagtca cggaatccag tactttttcc aatgaaaaat tacgaatgat gcgctgattg
     8461 aatatttcct tctctgaagc attcatagta gtgtccaacc attcagctag tacagacatc
     8521 ttttcccccg agatgaggtc ttctaatgaa agatagtcga tattggtgat atcaggatct
     8581 gtggtgccaa tcattcctac gacttcgtat atggaaaata atgggtcact gtttttctta
     8641 tcttttaatt gctgatattc atatgggatg attttcaact gacaagccgt aggtaagctt
     8701 ttttctagca gttcacttaa gtggaccgcc gtaccgattc cagtgaagca agtagtaatg
     8761 agtgctttgg gtttgttttc ctctggatag agcagtcgcc attctggctg gatctgtcga
     8821 acagtctctt ctgctatctc ttctaatgat acattttttt gcaggcattc ccctactgtg
     8881 attgctaaag gtgtcgtcac attattcaaa atcaaaagcg gtacggataa ctgtttttta
     8941 aaaaactgat ggatttcttt cagagatccc atgtctacta atatgatcaa tccatttgaa
     9001 acatcgctgt tttcgcaata atcaaggatt tctgaagcaa tctgctgtgg tgtgacatct
     9061 agtggcatat caaatgattc aaatagatcc tcttgtaata aacgatttac tacatttgcc
     9121 atactgctag cagttgcata accgtgagca gcaatcatcg cttttggcag tcgtttttct
     9181 tttgaccatt tcgctttttt tagatagatc gtcagtaaaa tccgatccat acttgtgtct
     9241 tcaatatcta atttgatctt acttaattcc agaattcgtt ggacatagtg ataactagat
     9301 ggataagact gagaaacttg ttgttctaat tctttgatta gctggataat atcatgttct
     9361 tccggtaacc atttgatagt gcttcgctga tagagatagt aactcaaggc atagacactg
     9421 tttccatcga atttgatttg ataggcagtt tccatttgtc gaaaggtctc tcggatagat
     9481 tgtgtccaat aaaccagcag ctcgtgtttt tcttgtctac tggtctcaaa cagcaaaaaa
     9541 tcaaataatt gaatcacttc ttgctttaat tttccttcgc aagaatatag cagacagtta
     9601 tttttcttga aatcaattag gatttgctca aaacttttaa cgattcgctg ctgttcgggc
     9661 tgttgcttct ccattaattg ctctaaggtc tggtcttgcg taatatagat tccttgctgt
     9721 gctgtatttt tagtaatagg ctgcgcaagt agttcttttt gcaaatgatg gatcgtgaca
     9781 tggatttcag gctgttcacg ctgttcagca tatgcttttg ctgctgaaac tttgacgata
     9841 tttttcagct ctcctatgtt tccggataat ttctgacttg ttaaaagtga caaagctggt
     9901 ccactgactg acaaagggct ccccattttt ctttgttctt cgagaaaaaa agagtaaatc
     9961 agttctagcc gttcattacg actacgctga ttaagtgcag gtagttcgat tcggataggt
    10021 attcttcgaa tgaaagttgt taagaaatga ctagtaatct cttcagtcgt agcaaaaaag
    10081 agacgtgttt tgatccggcg tgacaaatta ggttctccca tacgataaat cacaccttga
    10141 tcaagatagg tgaacagttt ttcctgacct tctggtgtta agcgatgtac ctcgtctaaa
    10201 aacaagatcc caccatctgc tgcttcaaaa gccccttttt tattttcttc tgcaccagtg
    10261 tacgctccct taacatgtcc aaacaaattg ctagtcaaaa gttcaggatt atctgcatat
    10321 tgtgcacaat taaaagtgat gaatggtgca gaatcttcta gcagatcatg aagcagacaa
    10381 tactgataga ccaaattaac taaataactt ttacctgtgc cactttcgcc tgtaatcaaa
    10441 agtggtaatc cgccatctgg atagtaaaga gctgttttga tttgttcgat actttctttc
    10501 aaactggcgt cgtatccgat cataaaagag aataagtctt ttggttgcgc gaaaaaaggc
    10561 tgttcctctt tgagatcatc gatagaaaaa taaaaattcg tgcgtaactt aaaaaattgt
    10621 ttttcaaaag cttctttgtg gaaataaatc actggtcgag aattgatttt gactagtagt
    10681 tgctcatcat ttaactggtt caaataatga ctgactgtgt ttctttttac cccaaaatga
    10741 tcagctaacc tttgtgctgt aaagacctcg ttcatatgtt ctaaatcgaa aaaggctgtc
    10801 tggttctcta agtaacataa aatttccttc ttcaacaact tattcacccc tcctccatca
    10861 tagaaaacgg attcattttg tcaataaaat ctttaataaa gaattgaaaa atatatctta
    10921 ttatagacag cagcctactt atcccttata atttttattc taaaaaacgt acagttcctt
    10981 ccagttttta tactggaatt tcaacatatg aaagaataag gaggctatga ctattcaatg
    11041 aaatcggata aatataaaaa caggaaacat acttcctgga aaacttttcg agaaatcatc
    11101 ggatcttatc tcgctgtagt tgtatttttg cctttgcttt atatggctat gacagaacaa
    11161 actcgtttta ttggcgtaga tacgcaatat atagccgaaa caggattttt attcatcttt
    11221 atcttgattt ttgcgttcta taaatccttc catacaaaat aagattcaca gatttcaact
    11281 ttcctgtctg tttttagcta ttacaaatcc ttatatgtgg tataacaaca atttattggc
    11341 ataatgagga aaaacgagtt atgatgcttt cagagagtaa agaggtactg ttgtcaccat
    11401 cctttttatt gattacgtac accactctta gttttttccc attgattacg aaaacaaggt
    11461 agaaacattg atgttcctac cttgttttct ttttttattt tcagatcaaa atctcaagta
    11521 gcttttgact tgatgtcggg aggttgtctt cgttccatca aacctgatag aatctgcgcc
    11581 aaaccatagt tttctccact attatttatt cctctaagat agtacccaca ataaacgaat
    11641 aaatattttt ctcattttca tactccttct ttgaaaatca attagatttc tctcaaccca
    11701 cttttttaca aaaattacgt agcaaaaaag attgcaagaa caaattaaaa aagaagtaaa
    11761 tagagtttca tctataaaca tttacttgat atatttttta tgcagaattc atttatagaa
    11821 aaa
//
LOCUS       DAGCXG010000081        11579 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGCXG010000081 DAGCXG010000000
VERSION     DAGCXG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.084"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            237..1877
                     /locus_tag="MB022_002096"
     CDS             237..1877
                     /locus_tag="MB022_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBT4467080.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="MB022_002097"
     CDS             2031..3353
                     /locus_tag="MB022_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4467081.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="MB022_002098"
     CDS             complement(3415..3846)
                     /locus_tag="MB022_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467082.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="MB022_002099"
     CDS             complement(3910..4746)
                     /locus_tag="MB022_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4467083.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="MB022_002100"
     CDS             complement(4936..5238)
                     /locus_tag="MB022_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBT4467084.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="MB022_002101"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="MB022_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBT4467085.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="MB022_002102"
     CDS             complement(5692..5958)
                     /locus_tag="MB022_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBT4467086.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="MB022_002103"
     CDS             complement(6212..6937)
                     /locus_tag="MB022_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBT4467087.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="MB022_002104"
     tRNA            complement(7303..7373)
                     /locus_tag="MB022_002104"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="MB022_002105"
     CDS             7490..8242
                     /locus_tag="MB022_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4467088.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="MB022_002106"
     CDS             8258..8737
                     /locus_tag="MB022_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4467089.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="MB022_002107"
     CDS             complement(8988..10568)
                     /locus_tag="MB022_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBT4467090.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="MB022_002108"
     CDS             complement(10561..11304)
                     /locus_tag="MB022_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467091.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..>11579)
                     /locus_tag="MB022_002109"
     CDS             complement(11480..>11579)
                     /locus_tag="MB022_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBT4467092.1"
                     /translation="VTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3518 a         2312 c         2056 g         3693 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaatctt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttact
//
LOCUS       DAGCXG010000082        11419 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGCXG010000082 DAGCXG010000000
VERSION     DAGCXG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.085"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            93..1064
                     /locus_tag="MB022_002110"
     CDS             93..1064
                     /locus_tag="MB022_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBT4467093.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1142..1378)
                     /locus_tag="MB022_002111"
     CDS             complement(1142..1378)
                     /locus_tag="MB022_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4467094.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1347..1478)
                     /locus_tag="MB022_002112"
     CDS             complement(1347..1478)
                     /locus_tag="MB022_002112"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4467095.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1554..1937)
                     /locus_tag="MB022_002113"
     CDS             complement(1554..1937)
                     /locus_tag="MB022_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4467096.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(1970..2755)
                     /locus_tag="MB022_002114"
     CDS             complement(1970..2755)
                     /locus_tag="MB022_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBT4467097.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            2933..4033
                     /locus_tag="MB022_002115"
     CDS             2933..4033
                     /locus_tag="MB022_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4467098.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4023..4847
                     /locus_tag="MB022_002116"
     CDS             4023..4847
                     /locus_tag="MB022_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBT4467099.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            4844..5659
                     /locus_tag="MB022_002117"
     CDS             4844..5659
                     /locus_tag="MB022_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4467100.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5656..6486
                     /locus_tag="MB022_002118"
     CDS             5656..6486
                     /locus_tag="MB022_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467101.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6493..7524
                     /locus_tag="MB022_002119"
     CDS             6493..7524
                     /locus_tag="MB022_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467102.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7631..8904)
                     /locus_tag="MB022_002120"
                     /pseudo
     CDS             complement(7631..8904)
                     /locus_tag="MB022_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9181..9390
                     /locus_tag="MB022_002121"
     CDS             9181..9390
                     /locus_tag="MB022_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467103.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9440..10156
                     /locus_tag="MB022_002122"
     CDS             9440..10156
                     /locus_tag="MB022_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBT4467104.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10058..10578)
                     /locus_tag="MB022_002123"
                     /pseudo
     CDS             complement(10058..10578)
                     /locus_tag="MB022_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10866..>11419
                     /locus_tag="MB022_002124"
     CDS             10866..>11419
                     /locus_tag="MB022_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4467105.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPY"
BASE COUNT         3252 a         2135 c         2635 g         3397 t
ORIGIN      
        1 ctagtatatt tatatgagct aattttatga ttaaatggta agtttttcct tgtgtgtttt
       61 agtttaaact agtgtgtaat tcaaaaatct atatggaagg aacagtatta agaatagaac
      121 agggttctct acatgatggt gctggtttac ggaccgttgt ttatttaaag ggctgcccat
      181 tacgctgtgc ctggtgttcg attcccgaat cacaaagcaa gcagattgaa aaaggatttg
      241 gccagaccat gaccgctgaa gaggtaatgg atgagattga gaaggatgct gttttttact
      301 ttcactcgga tggcggagtg accattagcg gaggcgaggc actggttcag gcggattttg
      361 ctaaagaaat tttgcaaaaa tcgaaataca ttggaatcaa taccgtactg gaaaccagct
      421 tttgtggtgc ttataatgaa atccaaaagg tagctcccta tgtggataca ttgtttgtgg
      481 atgtaaaaat gtttaccagc aagctccaca aacaatggac cggcttagat aaccaacaaa
      541 ttttgaagaa tattcgtcgc tttttaattg agtatcctaa ctgcgaagtg cgaattcgag
      601 ttcctgtggt tccaggtatc aatatgaacc tgaccgagct gctgacgatt gcttgttttg
      661 tggcagatct tgatcggttc gtaccgttag aattactacc gtaccactgc tatggtatgc
      721 acggttacca agcattggga ctggagtatc cgttggcaga cacgccagcg ccttcaccta
      781 aagaaatgtt tgcattggca gatcaactgg ctcgcacggt tcctcatttg ctggtcacag
      841 ctttaaccaa aacatttatt tatttattga atccaagctt tatcttatgg ataaatgaaa
      901 gacactcact agctgcagcc agctcaagaa tagagctttt gctgaagtca gtgagtgtct
      961 ttttacgatg ttttttcgaa gttaatttgt cgaatgaaca ctggctcaat cggtacatct
     1021 tcactgtttg tcggctgagc tgcgatcgct gctacgattt ctaacccttg gtatacctgt
     1081 ccaaaaacag tataacgacc atccagctcc ggtagtcctc caagctccaa gtatgcttgc
     1141 tttacattag caggtcagcc tgcgcctttg tgttttgcac aataaaaaac tggttgccgt
     1201 tggtgtgggg gccgtggtcc cctaaggcca aggcgccatt aaaatgataa agtccgcgat
     1261 gcacttcatc agcgaaaaag ccctcatagc tggaaacagc ttcatcacca gaaccatcca
     1321 aagcgccacc ttgaataaca aattccttaa tgacacgagc cgaaaggtgt gttgtcataa
     1381 acgccttgct cagctaggga gcgccagttg gctacagcca ggggagcttg gtcaggaaat
     1441 aagcggagac gaatttcacc agcatcggtg accagcacac aaacttcttc ctgctgcggt
     1501 ttttctaatt gaaagagagg aaggtttgca actgaaacgc cttgatcctg ccatcaaata
     1561 tcgaaaatcc gccactcgac cttcgcctga tcaccaaggc gagccattcc caataaaacg
     1621 gtataggcgg tttcatccgc ttgaaaggtc aattgcagct gtcctcgtaa atcgttgtag
     1681 tcatctgggg cggctacttg ataaaaaaga tgaaactgcg catgcaaata ttccaaaggc
     1741 tgatctttaa aggctgcaaa ataagcctga actttttccc gctgcaagct gtcagccacc
     1801 aaaaaatttt ccagttgttg ttttggaacg gctgctaaca aggatcggca aaccgcctca
     1861 attttcaacg catcccaatg tcgcgcaatt aaataatgga tgggtgcctc ttgattgtag
     1921 tcccacaggt tgctcatttt tttcaccact ttcttctatt atataggaat cacaaaatcg
     1981 gcagcggcgt caggcggcgt tcttttcctt gaatgactag caacttttct acctgacagt
     2041 ctgccaacag ctgactcatt tttttaaagc ccagctgata atcttcggca acatgagcat
     2101 ccgagcctaa ggtaaagagc tttccgccga cactttgata caaaggaacg gcataacggt
     2161 aaaggtcttc gttgccgtat ttcaagaagc tcttggcatt taactccatc gccaattctc
     2221 gttcaacaac ctttttgaaa atcgttagca gctgagcctc aaaatgctcc gcgaattctt
     2281 ccgctgtgaa ggtgaagcgg cgtaatccat aatcaaagtg agtcaaaata tctccttctg
     2341 gaaaatccgt caagacctgc aacatctgat caaaatattc cttggtagtt tgaatgggat
     2401 ctttggttaa aaccacatcg tccatataat caaagcggcc attttgatga atacttacca
     2461 gctttaaatc ataaggatgc tgagcgaggt aatccacgat ccgctcttct tgtcctggaa
     2521 caacgccaat ctcgatacct cgcagaaatt tggtggaata ggtttctttt aaagagtcca
     2581 gtttgtttat ataggctgga taatccggaa tgtcatcttt tccggtagat ggattgttta
     2641 aatcaaagtg atccgtagaa acgaaaaact ccggctgata ggctaaatag ttttcaaatt
     2701 tttcctctga atcaaaggaa aaatacgtgt ggagatgctg atcataatac tgcataggga
     2761 gcctcctttt ttctactatc ataccgcaaa agctttagaa ggttaacggg aattcgtcga
     2821 aatttacaaa ccatatacaa cctttacatt gttcatgaaa ccttttcaag aaactgattc
     2881 tctcattaca aagctttgtt agaatggaat tatcgaaagg ggagaagaat gaatgaaaat
     2941 gaatgaaaaa attcgtaaca tgactatcgg tttattttgt gcagcaacag taagtatcct
     3001 gctgacaggc tgcgtctcag gtgaagcaac gaagcaatcg gctgcggcag acgactcttt
     3061 agatacgatt atagaaaaag ccaaggaaga aggagagatt gccagcttgg gaatgccgga
     3121 tacttgggca aattgggtag gcacttggaa cgaccttgaa aaagaatacg gcttaagtca
     3181 cgtggatacg gatatgtcca gtgcagaaga attggctaag tttgaatcag aaggaaaaaa
     3241 tggaaccgcc gatattggtg atgtcggtat cagcttcggt ccgttggctg aaagcaaaga
     3301 gttaacctta ccttataaga ccaactattg ggaggaaatt ccggattggg caaaggatga
     3361 tgatggggat tggttgttaa gctatacggg aaccatttcc tttatcaccg ataaagagaa
     3421 tgtgaagcaa gcaccgacta gctggaagga gctgcaagag ggtgactatc aagttagcgt
     3481 gggggatgtg acgattgcca accaagcaca atttgctgta ttggctgcgg cgattgccaa
     3541 cggaggtgac gaaaaggata ttcagccagg tctgaattac tacaaaaaaa tggcagaaaa
     3601 aggccgtttg tccaccgttg atgccagcgt agcaaatctt gaaaaaggtg aaatcgatgt
     3661 ggcagttgtt tgggacttca atggactaaa ttatcgtgac caaattgact ccgagcgttt
     3721 tgaggtaact gtgccaacag acggctccat cattagcggg tatacaacct taatcaataa
     3781 aaatgcccct catcccaatg cggcaaagct agcacgggaa tatattttat ctgatgaagg
     3841 gcaaatcaat ttggcaaagg gctatgcgcg acctattcgc aaggtagagc tgcctaaaga
     3901 agtagctgaa aaattactgc cggaggatgc ttatgaagcg gcggtgccag ttgaagacaa
     3961 tgaagcttgg aacaagacct ctgtagagct tccagagctt tggcaggcgg aggtactgac
     4021 ctatgcaaag tagacttgac aaactgattg tagttatact agacggctgt cgctacgaca
     4081 cagccgtcgc ccagcttggg ttcatgaatc atttagttga gcaccagcaa gcaagcttat
     4141 tgaaggtaaa aagtgagctg cccagcaatt cacgtccgtt atacgaagta ctgatgaccg
     4201 gcgttccagc ctatgaaaat ggcatctata gtaattacca tgctcagcga tccaaagagt
     4261 ggtcgctgtt tgaattagtt aaggaagctg gcggaaaaac aggagccgcc gcttaccact
     4321 ggtacagcga gttgtacaat caagccccct ataatcgttt ggttgatcgc atccagcatc
     4381 aggaggagcg actgattcag catggcatct tttatagtga ggacacttac cctgattcgc
     4441 atttatttgc ggatgcccat tatttagtga atcagtatca gccagacttt ttggtcgtac
     4501 attcgatgaa tattgatgat gtcggtcaca aatttaccgc cgattctcat gaatatgcag
     4561 cagcggttaa tcaggcggac agattattgg ctatttacct tcctgaatgg ctgcaaaaag
     4621 gttatcaagt cgtggtgact gcggatcacg ggatggatgc gcacggactg catggcggca
     4681 gcttggctgc acatcgtgaa gttccttttt atttgatttc aaataaacgc caaaagctga
     4741 aggaacaaac tgtaccacag ctgctggcgg ctccattgtt ttgctggtta ttaggaattg
     4801 caccgtcaga gaaaatgcag gacattcaag aattgatgag gggatgaaag agatgaaaaa
     4861 acgcttggtt ctggttcctg cctcctttat cttctgtgca tttttactgc tgccgctgct
     4921 ttttatggtt ctgtgcagct tgcacagtga tttaactaac cagtggacat tggccaatta
     4981 cagtgatatt ttcagcaaca aatattacac acaggccttt gttaacagcg tactgattgc
     5041 tgtcagctcc agtgtgattg ggttaatcgg gacgtttatt ttatgtttat gcttaatgaa
     5101 tctatcgact aaattgcagg agaaaatcac ctttgtttcc aatttggcag ctaattttgc
     5161 tgggattccg ctggcattct cttttattat tttgttgggg aatgtaggtg tgctgaaaat
     5221 gattttgcca tttctagcgg acttcaattt gtattcttgg tggggattga ccatcacgta
     5281 tacctatttc caaattccgt taggggtctt gtttttgtat cccagcatga aggaagtgaa
     5341 aaaggaatgg ctggagtccg cacaattgct gggcgcttca tcagcgtatg cttggttcaa
     5401 ggtggctttg ccttttttgc gaccatcgat tgccagcacc tttgtcatct tatttgctaa
     5461 tggcatgggg acctatgaaa cagcgtatgc cctgaccagc agtaatgtaa atcttttaac
     5521 gattcgaatt gcggctttag tgtcgggaga tgtgttcgcc aagcctaatt taggcagtgc
     5581 gttggcagtc gctttaggtg tcctgctggt aacggcaatg ttgattattc aaaagaaagg
     5641 caaggtggtg cagacgtgaa aaagttaata aaaccgattt tgttctgctt tgtcctgtat
     5701 tacttgctgc cgattattgg aacgattttg tatgccagct ccactaaatg gtcaaaatcg
     5761 cttttaccta gtgattttac cttgcaatgg ttccaacagc tgctgacgga cagggaattt
     5821 attgctgcgg tgggtcgctc acttttatta gcaggtgtgg tcttggtgac gattctgttg
     5881 ttgatgattc ccaccattat ttggattcat ttgtatttcc ctcgtttgaa tcgttggctg
     5941 gaaaagctgc tcctgctgcc gtatgcttta ccaggagtga ttttggtaac ggctttgctt
     6001 cgaacgtatg ctgaaactgg tatcccaatg tttgtagtgt tggtaggtgc attgtttatt
     6061 acggcgttgc cgattgtcta tcttagtctt aacaaccaga tgcgcttaat taatctgaag
     6121 gaattagtgg atgcagcaga aaccttgggc gccccgatgt ctacgattat tatccaggtg
     6181 ctgtttccaa acatccgtat cggtgtgacc ctagtttctc tgatgatttt ttcttcggtt
     6241 tttggggagt acatgctgac gaacctgttg attggcggtc gatttcagac ggtacgcatc
     6301 tatatgctgc ggcggatgaa cgaaaacggc catttggcta gcgctgtgat ggttttgtat
     6361 ttgctgttta tggtgggaat tgctttaact acctttatgc tgacttatcg acaaaagcaa
     6421 cagctgtccc ttaagcagac gagggaagag aagcagtcga aaaagaagcc cttaaaggag
     6481 ttgtgacgga aaatgttttt agaaatacat gatctcaatc tgttttttga tgaaaaacag
     6541 attttagcta ttgatgaaat tcagattgaa aagggtgagc tggttacttt actaggtccc
     6601 agcgggtgcg gaaagtctac gttgctgcgc tgtatcacgg gattggaaca accaaagagc
     6661 ggtgagattg tgctggacaa tgagaatatt gccgataagc ctacgaagga tcgcaatatc
     6721 ggctttgttt ttcaacagta tgcattgttt ccaactatga ctgtttttga aaatgtagcc
     6781 tttggtttga aggtaaagaa gctgagtcct gaggtgattc aggaaaaggt ttttgagatg
     6841 ctatcattgg tggatatgac ggagcaggca gataaaaatg tccagtttct ttctggcggt
     6901 caacgacaac gggtggcact ggctcgctca ttggtcactg aaccgaaagt cctgctgctg
     6961 gatgagccat taagtgcttt ggatgcccga attagaaaac agctgcagcg ggatttgcgc
     7021 gctatccagc aatcgctagg gatgacgatg atttttgtga ctcatgacca agaagaggcg
     7081 atgcgaatca gtgatcggat ttttgtgatg gaagctgggc gggtggctca ggtttccacc
     7141 tcgaaggaat tgtatcagaa tcctcagagc cgatttgttg cggaatttat cggcaactat
     7201 aatcgtttcg aatggtatga gctgaatcag tatacccacg agttttccgc taagggcaat
     7261 cgctgcattt attatttaag accggagctg attcaatttg aaccggttcc tcagggggtt
     7321 aagattccgg ttctatggaa ggaatcattt attttaggca acattcaacg gtatgttttt
     7381 caaacgctgg agggcaaaga aattttagtg gatcggttga acgatcggga agagaaaatt
     7441 agcgatgcct tatacattca gctgaaagat attctagcaa tccctgtgga aattgatact
     7501 cagcattttg cacaacaaag ctagtgtata gtcggcaaac atttctggct ctttgtcaaa
     7561 taggactgat agagtgatat aagaacatct aaggtagaat tgattttctg ctttagatgt
     7621 ttttttatgt ttaataagtg atcagatgat tgattaagaa aattctggtt ctcatcctaa
     7681 cccgtaagct attcgtttca atgctttgat gtgggtatgc agattttcta ttttgccatt
     7741 tgagtaaggc aactctaagg ctaaacgaat gccggattca tagtttataa acgctttttt
     7801 tatatgatgc agttctcttg gaaccgactt gggcattgtt tttacaagct gaaagaagat
     7861 gtccgcttct ttgtcataaa aagcttgaag aatttcttga tagtaagcat aagctattcg
     7921 tagttcatca gaagtagaaa gtagacgttc tacgacttca ctttcggtta aataggccca
     7981 accaaaatta cgccattttg tccatttatc ataaacaagc gtgtcctgtc gtttgatcag
     8041 aagtcgagat aaagccttca cttgtcgata gtgtttggct tgtattggat cgtttcggtc
     8101 gaagcttttc atgactcata gtcaaatatc cttaaaactg cgaatgaggt gcttgacgat
     8161 gtgaaagcga tccacaacga gtttggcatg aggaaagcac tcttttatca gcaaagcata
     8221 agaggcgttc atatctgtta cgagtatttt tacgtttaaa cgtgcacgtc gttcaaattg
     8281 cataaaataa gaaatgagat ctttcttctt acgtgaagga agaatatcca gtaatttccc
     8341 agtatcgcca tccatacaag aaaaagaata gggaccgact tgatttttca atgaacgaaa
     8401 ttcatctacc aatgaacagc gaggcaacca atttattttc acgtttgtca tggggagcag
     8461 agaagcgagt gttcgcgaaa cggttgacca agaaacgccg caatgtttag aaatcgtcgt
     8521 cattgcttgt ttttcagtca attcctccaa tattttataa tgtacttgtt tggcgataaa
     8581 acagtttttt tcaacaatgt aggtattcgc cgtaaaatag gtgtgacagc cacgacagag
     8641 gtaccgttgc tttgccaaag caagataagt ccgttgattc gcacacttat tgagaaggac
     8701 cagcgatttt ttctttccgt tcttaacgat cagcccatgt tgattcacct gtttacactg
     8761 aggacagggc tgaatcgttt taatgagcgt ggcagaaaca acgatactgt tgattgaatg
     8821 tatttttttc ttctcgacat gagtgatttg aatgtttcca tctttgattc ctagtaatag
     8881 tttgataaga tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa
     8941 ttctaacgga gcttctgtat gttttgtttt tttatgctca aaaggactga tagaatgatt
     9001 tactatcagt cctagaaatt atagcgccac atttctttca cctttgactt ttcagacggc
     9061 agtttttatg ctgatactgt agagagagca ggacacagct tttgcttgtt ttgggtaagg
     9121 gggaaagaag atgaccaaag cacttatttt tgattttgat gggacgatga ttgacagtga
     9181 atggatgttt tacgagagta ttcatcacta tttactgaag cctatttact ggagcagttc
     9241 ggctataaaa ttccttttgc agactatcaa caaggtgttg ggacggcagc agaactgtcc
     9301 ttttatgaag ggataagtca agcggttggg agaccagtgg atgctgaaca ttttgagcag
     9361 cggatcagca gcactggaaa atcggagtag tctcaattct gaactgcggg agctagacta
     9421 tttttttgaa gcacatccga tgattaagga aaaaattgat tcctttgtga cgatcgatga
     9481 tcttcaggtc ggcaaaccag atccagaagg ttatttgttg tgcttgaaac ggctgagggt
     9541 tgaacaggct gttgtagttg aggatagtcc agtcggtgcc caagcagcac cgcatatgac
     9601 agttgatgta caagcgttgg atgcagattt tttcgccttt agcgggcata aaatgtgcgg
     9661 gccgacagga atcggtgtat tatatggcaa acgtcaattg ttagaacaaa tggagccagt
     9721 cgaattcggc ggtgaaatga tagattttgt caatctttac gatagttcat ggaaagagct
     9781 gccatggaaa tttgaagctg gtactccgaa cattgcagga gcaatcgatt acttgaatga
     9841 gattggtatg gagaatattc atcgttatga gcaagaactg gttgcctacg ttttacctaa
     9901 attacaagcg atcggcggta tcaccattta cggaccgcaa gatcctgaac atcatactgg
     9961 cgtggttgct tttaatttgg atggcatcca tccccgtgat gtggcgactg ctctggatat
    10021 ggaaggtgtc gctgtacgtt caggacacca ttgtgcacaa cggttcatcg acgatttctg
    10081 tttgactaat tggtcgttta aacagtcgat gataatgccg tttgagcggc tccaatgtcg
    10141 tggaatcttt gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt
    10201 cagattacgt gattgatttt cgtacagttc tatgagaatc gcctgcttca gttcctttgt
    10261 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtctgcc cacaatcata
    10321 gcatagaaaa cgggtacgtt ttaattctaa aatagtcaac ttttctttga ctttcaacat
    10381 ctgagtacgt gtcgtatagg aaccgttgcg gataatacga atcgatccac agtgttcaca
    10441 atgggaaggt gagtcatcca atctcccttt aataaactgc gcttgaacgc ctttatattc
    10501 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
    10561 tctagtatga ttatccatag agcgactcct ttggtagggt gatgttttgg tcgactaaat
    10621 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
    10681 ccttcgattt tccaacaaca caaaatatta tagaagcctt ttttctaaag gatttcaagg
    10741 tgtctgtttg catttattaa aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca
    10801 ctagatatta tagagccata atatgctgta taatatctag tgtataggct taagtggagg
    10861 tatatatgaa aatattaact gttgaagatg ataatatgat aagagaggga ataagtgaat
    10921 atctttcaga atttggatat actgttattc aagctaaaga tggaagagaa gccttgtcaa
    10981 aatttaatag cgatataaat ttggttatct tagacattca gatacctttt ataaatggtt
    11041 tagaagtgtt gaaagaaatt agaaagaaaa gcaatttacc aattctaatc ttgactgcat
    11101 ttagtgatga agaatataaa attgatgcat ttactaattt agtagacgga tatgtagaaa
    11161 agccattttc attgccggtc ttaaaagcta gaatagattc tttaattaag aagaattatg
    11221 gacatttaga gaagtttgaa tataaggatc tatcggttaa ttttaatagc tatacagcta
    11281 aaataaatgg tgaagaaata gatgtaaatg caaaagaact agaagtatta aaatgtttat
    11341 tggataataa tggacaagtg ttaacaagaa tgcaaattat tgattatgta tggaaagata
    11401 gcgaagaaac tccttatga
//
LOCUS       DAGCXG010000083        11300 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGCXG010000083 DAGCXG010000000
VERSION     DAGCXG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.086"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            188..1447
                     /gene="lysA"
                     /locus_tag="MB022_002125"
     CDS             188..1447
                     /gene="lysA"
                     /locus_tag="MB022_002125"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBT4467106.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1434..2234
                     /gene="dapF"
                     /locus_tag="MB022_002126"
     CDS             1434..2234
                     /gene="dapF"
                     /locus_tag="MB022_002126"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBT4467107.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2237..3118
                     /locus_tag="MB022_002127"
     CDS             2237..3118
                     /locus_tag="MB022_002127"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBT4467108.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3115..3831
                     /locus_tag="MB022_002128"
     CDS             3115..3831
                     /locus_tag="MB022_002128"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBT4467109.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3837..4532
                     /gene="dapD"
                     /locus_tag="MB022_002129"
     CDS             3837..4532
                     /gene="dapD"
                     /locus_tag="MB022_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBT4467110.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4548..5549
                     /locus_tag="MB022_002130"
     CDS             4548..5549
                     /locus_tag="MB022_002130"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBT4467111.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5546..6907
                     /locus_tag="MB022_002131"
     CDS             5546..6907
                     /locus_tag="MB022_002131"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBT4467112.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6867..8018
                     /locus_tag="MB022_002132"
     CDS             6867..8018
                     /locus_tag="MB022_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBT4467113.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7993..9162
                     /locus_tag="MB022_002133"
     CDS             7993..9162
                     /locus_tag="MB022_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4467114.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9337..10716
                     /locus_tag="MB022_002134"
     CDS             9337..10716
                     /locus_tag="MB022_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4467115.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10997..11300
                     /locus_tag="MB022_002135"
                     /pseudo
     CDS             10997..11300
                     /locus_tag="MB022_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3761 a         1975 c         2519 g         3045 t
ORIGIN      
        1 taaatagctt ctaatagttc gggtattttg tttttataac catttttatt cttattttta
       61 aaaaaatatt tcgaaatata ctagacaatt cagaaaattt tgtttaatat tattccaata
      121 aaattcaaat ctttatcaaa tgaaattcca ataaaaacgg aaaaaaagtg aaaagaggaa
      181 tgaaaaaatg acagaattga taattggcgg agtcacagca agcacattgg cagaaaagta
      241 taagacacct ctgtatgtgt atgatgaaga aaagatgaga caaacgatgc tgtcattcaa
      301 gcgaggcttt gtatcgaaac gttttgatac aaaagtgctt tacgcctcta aagcattcca
      361 aacgattgag atgctgcatt tagttcgtga atatggattc gggctagatg ttgtcagtgg
      421 tggggaaatc tataccgctt tgcaagctga ttttcctaga gatactatct acttccatgg
      481 taacaacaag acgccagagg aactgatcta tgcacttgaa aatgatttgt tgcatattgt
      541 tgccgataat ttgatggaag ttgaattatt agcagaatta agccaacgct atttaaaaaa
      601 aatctgtatc atgcttcggt tgaatgtcgg agtagaagca catactcatg aatatatcgt
      661 cactgcgcat attgattcaa aattcggcat gagctatgaa agtaaagagt gccaacaagc
      721 ccttcagttg ataaaagaaa gcagctatct agagttagaa ggatttcatg ctcatatcgg
      781 gtcacaaatt tttgatgtta ctgcttggtt agctgaaatc gacaaattga cgaattactt
      841 aaccgatttt gaccaacctc tttccttgaa tcttggcgga gggtttggga ttcgttatac
      901 acaaaatgat cagccgatcc cagtcgaaga ggcactaaaa aaactgattg aatatacgga
      961 agaagctttg gaaaaacgaa aactgaccat tcgtcaatta ctgatcgaac cgggtagaag
     1021 tattgttgga gaagctggta caacactata cacgattggt ttcatcaaaa agacaccaca
     1081 taaaaaatat tactttgtag atggcgggat gacagataat atccgtccag ctctgtatca
     1141 ggcagaatac gattgtgatt tagcagcaaa actgtcggaa gaaaaaacag agaaagtgac
     1201 gattgcagga aaaatgtgtg aatcaggcga tgtggtaatt aaagaagctt atttgccgaa
     1261 agcagaatcg ggtgatcttt tagctgttta ttccacggga gcttatggat actcgatgag
     1321 cagcaactac aatcgagcga cacgaccagc agttgttttt gtcaataatg gccaatcaag
     1381 actgattgtt cgcagacaag cattttctga tctactaagg ggggaagttt cctatgatcc
     1441 ttcataaata taatggctgc ggaaatgatt ttatcttatt agattacgca gaagacacag
     1501 attatagtca attagccgct tctttatgca caaaagaaaa atatgataca gacggattga
     1561 tcgccgtaaa aatcgatccg ttggaaatga tctattacaa caaagatggc agccgcgcgc
     1621 cgatgtgcgg aaatggcatt cgctgttttg ctcgatacgt aaatgatcag aacatgatga
     1681 atgaacagga attcaacgta gagacattag caggcacaag aaaaattacg gtgttggatc
     1741 cctcaccttt ttattgcagc gtagatatgg gagtgccaga tgattctccg gaaaaaatca
     1801 aagctaatca aacttatcca ataaaaaacc aggaaataga gatcaatggt gaaaaagtaa
     1861 cgatcactag tttattttta ggaacgattc ataccgttgt ttttgttgaa aatgcgcgtg
     1921 aggaagtctg gagaaaaaga ggtgaactga tctgtcatca tcctttgttc aaagaaaaaa
     1981 ccaatgtcaa ctttgtagaa gtagtcaatg aacaagaatt gatcgtccgt acctatgaaa
     2041 gaggagtagg ttggacactt gcttgcggta caggttgctg tgcctcttat gtggttgcta
     2101 aggaaactgg gaaaatttca gcagatcagg tagttgttca cttggaacaa ggagacttga
     2161 tgatctctgg tgaggatgcg atacaaatgg ctggcccagc agttcatgaa tggacaagaa
     2221 tattggagga atgaagatga taaaaggttc gatcgtggcg ctgatcacgc cgatgaatga
     2281 agagggatca gtagattatg ctggtttgga aaaattgatc caattccatt tagatgaaca
     2341 aacagatgga ttattagttt taggtacaac aggagaatct tctacgctca cacaatctga
     2401 ggaggagcag atccttcaac tgacagtaaa gaaagtggct ggccgtgtgc cggtgattgc
     2461 aggagctgga acgaataata cgaaagaaac gattgaaaaa gcgaagcatt ttgcttcttt
     2521 gggggcagac gcacttttgg tcatcacccc ttactacaac aaaacaagtg atgcaggact
     2581 agctgctcat tttacagcaa ttgcagaagc ctcaccgatt ccgcttattt tgtataatgt
     2641 tccttctaga acaggcatgt cgattccaat ccatgtatta gtaaatttag cagaacatcc
     2701 taatatcatt ggtttgaaag aagcctcagg agatatggct tatgtgatgg atgccgctcg
     2761 gttgattgga gaagaattct tcctttacag tggaaatgat gatttgatcc taccagtaat
     2821 gagtgtagga ggaagcggtg taataagtgt atgggcgaat atccaaccta aaatagtcca
     2881 tgaattagta aaagatacgc aggacggaag atggcagcaa gcaaaagaaa agcaattaaa
     2941 tgcacttgaa ctgatccatg ccttgttcag tgagaccaat cctattcctg tcaaagcagc
     3001 catgtcatta ttagacttac cttccggacc tctacgcctg cctttagttt ctctttcaaa
     3061 agagaaaaag aaacaattag ctcagctttt gttaaaagaa aggacggttg aaaaatgaaa
     3121 atcggactga ttggcagtgg acagatggga aatagagtcg aagaagttgc acaagaaaga
     3181 ggagatacgg tccttctttt acggacggca cctaaagaag caactacgct tgcttttacg
     3241 gaactgccag aactgcttat tgatttttct catccagata atttagagaa aatcctttcg
     3301 tatagcttat catatcaact tccgttagtc attggcacga caggttatac acaatcacag
     3361 ttccaagaaa taaaagaaca tgccaaacat gtacctgtga tgtatagtgc taatttttcg
     3421 ttcggcatca tggtgatgaa tcaactaatc aagcaagcag ctgcaatgtt gcaaggctgg
     3481 cagatcgaac tgattgaaaa acatcatagt cgtaaaaaag atgcaccatc tggtactgca
     3541 aatatgctgc ttcaaacaat ccaagaagtg caaaagctgc agcccgtata caactggcaa
     3601 gggaaaaaaa gagaagaaaa ccaaattggt gtacacagta ttcgtgcagg ttctttacct
     3661 ggtgaacatg atgtattgtt tgctgctact gatgaaatat ttacgatcaa acatgaatct
     3721 ttttctaatc gaatctttgc tgcaggcgca gtagaagcag cggactggtt gaaagatcag
     3781 tcaccaggtt attacaaatt agaagattta ttaatggaca aaggggtgta aaaaacgtgg
     3841 aagctcaaga aatcatccgc tacattcaaa cggcatctaa aaagacgcct gtgaaagtct
     3901 atctgaatgt aaccgagcca atcgaattta aagaaagtaa agtttttggt gaaggaagca
     3961 gcttcgttgt ttttggtgac tatgagacga ttgctccggt tttagaagct gaatcagaaa
     4021 aaatcattga aatcgaaatc gaaactgctg caagatacag cgcagttcct ttgctagata
     4081 ttaaaaaaat taatgctcgt atcgaaccag gtgctattat cagagatcaa gtgtcaatcg
     4141 gcaacaatgc cgtgatcatg atgggagcga ttatcaatat tggtgcagta atcggtgaaa
     4201 acacgatgat tgatatgggt gctgtcttag gtggacgagc gacagtgggc aaaaactgcc
     4261 atattggtgc aggagctgtc ttagcaggtg tgatcgaacc agcatcggcc aaaccggtta
     4321 tcgtagaaga cggcgtactg gtaggtgcga atgcagtgat tgttgaaggt gtccatatcg
     4381 gaaaagatgc agtcgtggca gcaggagcag tggttttaga agatgtggca gcagaaactg
     4441 ttgtcggtgg aatacctgca cgtgtattaa aaataagtga tgatcagaca aaagaaaata
     4501 cagcattgat tgctgcgtta cgcgaattat agaggagatg gagaaaaatg cgcgaaatag
     4561 atacctgtat tgtgggggca accggattag tcggccaaac gatgctcaaa gtgttagaag
     4621 aatatgattt tccagtagga cgattgaaat tattagcttc aaaacgttca gccggtaaag
     4681 tttgtgtatt caagaaaaag aaacacactg tacaggaatt gacagaaact tcatttgaag
     4741 gatatgattt agcgctattt tctgcaggtg cttctgtatc caaagagttt gcgccaatcg
     4801 cacgaagtca gggactattt gtcatagata attcctcagc atggcgggaa gattcggata
     4861 ttccattaat cgttccagag gtcaatctgg aagactatac attgaatcgg ctgattgcaa
     4921 atccaaattg ttcaaccatt caagcggtac ttccgcttaa agctttacaa gaagcctttg
     4981 gtatccagcg agtcaattat tcgacctatc aagcagtttc tggttccggg caaaaaggca
     5041 ttgaagatct tcatcgtacg caaaatgacg aggaaccagt tttgtatcct tataatatta
     5101 gccaaacggt gattccggaa atcgatcagc ctttggaaaa tgggtataca aaagaagaac
     5161 agaaaatgat aaatgagacc cgtaagattc tgcatcagcc tgctttgcca gtatcagcaa
     5221 cctgcgtacg tgtgccaatc gaaaatggac atggagtctc tgtggcagtt caactgcaaa
     5281 aagagtttac gacggaagaa attagagagt gtctcagcca gtttccaggg attcaattag
     5341 aagatgatct agcagcacat aaatatccga cgagcattct tgctagagga acagatatgg
     5401 tttatgttgg gaggatcaga aaagacactt ctttagagaa cggattgttg ttctatacga
     5461 ctgccgataa catacgcaaa ggagccgctg cgaatgccgt acagattgct gcagctttaa
     5521 tgcagaaaat agcggaggtg gcaagatgag taatacgctg aaagtagcga aattcggcgg
     5581 aacatcgatg tctcatgaag agcaattcaa aaaagtccgt acaattgttc tttcagatcc
     5641 caatcgccaa gtgatcgttg tttctgcttt aggcaaacgt acgtcagatg atgacaaagt
     5701 aacagactta ctttatttga tttatgctta ccttcagcat catgttgatt ggacacccct
     5761 ttggaaaagg atttgtgatc gtttcattca agtaagagat gttcttgccc tgcagttacc
     5821 tattgaagat gaactagcac gcatacagaa acaactggaa gacgaaaaga taactgaaga
     5881 ttatttagta agtagaggtg aatatctgac tgctcaactg atggctgagt atcttggtta
     5941 ccagtttatt gacgcaaaag acctgatttt ctttgactat gatgggaaaa tcgatcagga
     6001 aaaaacagca caggaaatca caaaagcatt taaaagatat ccgaaaatgg ttgttccagg
     6061 gttttacggg gcgaaccctg caggaaaaga aaaattattg ggccgtgggg gaagcgacat
     6121 taccggcgcg atactggcga agatgttgca agcggaaagt tatgagaatt ggacggatgt
     6181 ttcaggaatc atgatggctg atccgcgaat catcgatcat cctaaaatca tcgaggaaat
     6241 ctcttttaga gaactgagag agatggctta tatgggggct aacgttcttc atgaagcagc
     6301 tgttttccca gttcaagagg ctgatatccc catccaaata aaaaatacca atgatccttt
     6361 cgctcaaggg acaaaaatat cgaatcacga aatagaaaag gaaaatggac tgacaggaat
     6421 agctggacgc aaggattttc tttccatcac tttattcaaa cgtcatatgt ccgatgaaat
     6481 cgggtttatc tggaaagcaa tgagcatttt tgctaaacat gagatatcaa tcgaacatat
     6541 tccttcaggg atcgacaata tcggagtgat cgtctcagca gaagcgattg ccgataagtt
     6601 atttctcatc acgaaagaat taaaagaaac acttggcgtg gaagaaatcg tcgtgatcga
     6661 agacttagct ttgatctctg tcgttggggg tccgcataaa gaattgattg gattatctgg
     6721 gaaagtgtta tcgatattga acaaattaga aattcgcaca tcgatcttat cacaaggagc
     6781 acaggagttg aatctgatca tcggtgtacc gaataatcaa tatgaaacag tagtgaaggg
     6841 tatctatgaa gggatggtga atacggatgc tgaaagaata tcgaatggca ctccatcaaa
     6901 ttcctgaaat cgggtttcat gaattcaaga caaaaaaata tttatatgaa caagtaaaaa
     6961 acagcggggc cgttatccat gaaatcggcg aaacaggact actcctttat tttgataatc
     7021 agcaagaaga aacgattgct tttcgaacag atatcgatgc actgccagtc aaagaagaaa
     7081 cgaatctctc ttttgcttct aagcatgaag gttatatgca tgcatgtggt catgacggac
     7141 atatggcgat gcttttaggg ttgactgact ttataactaa acacattgag gatcttattt
     7201 ataatgtagt actgatctat cagccatcag aagaaatttc cggtggcgca caaagcgtta
     7261 ttcagtcagg attgttggat aaatatgaag tacaagcaat atttggcttt catctatggc
     7321 ctggactagc taagggagaa gttttctctc gaccaggacc tttgatggca caaagcagtg
     7381 aaacagacat tatcattaaa ggccagtctg cacatattgc ttctagtgaa aaaggaatcg
     7441 atagtctgga ggcagctgta cgatttttga agaatgttta tgattttgac cggcaattac
     7501 cggcagagtg gaatcatcta atgaaatttg gccaaatcca aggtggaacg atccgaaatg
     7561 ttttagctgg agaagttatt gtatccggaa gtatccgttc ttacagttca gatatgcaaa
     7621 attatttgaa gaggggatta gaccatttag cagaacagtt tcactctaca gctactattt
     7681 cttttcgcta taatgacggc tatccagctg taatcaacca tagaggctta tatgcagcac
     7741 tgttagattc aaacaaacta catgaattag atgctccagt tcttcaagcc gaagattttg
     7801 gcatatatac gcaacaatat ccatgtgtat ttttcttttt aggtgtcggt gatacccctg
     7861 ctttacatga tccgacgttc gattttgata tggatgtttt agaaaatggt gtagaatggt
     7921 acaaaacgat tctttttaca gaaaatcttc ttgtaaaaat cgatagcagt tcaaaagagg
     7981 agcgagtaaa taatgatgaa tttatccaat cgatttagtc ctaggctttc tagaatagaa
     8041 gtatcaaaaa tacgacaatt cgatcagcaa atatcgtcga taccagatgt gatcaaactc
     8101 accctgggag aaccggattt tccaactcct gaacatgtca agcaagcagg aatagcagcg
     8161 attgaagaag acttttctca ctataccgga atgagaggat tagaggaact gcgggaagcg
     8221 gcttgtatat ttcagcaaca gcgttatgga ttaacttatg atccgcaaac ggaagttttg
     8281 acgacagttg gtgcaacgga agcgattgcc tctgcgttgt tatctgtttt ggaagaagga
     8341 gacaaggttc ttattccagc gcctgcttat tcaggatatc agccattagt ggaactagct
     8401 ggtgctgaat tgatcccgat agatacttct gacacagggt ttgtctgtca gccggaacaa
     8461 tttgaacgag catttgaaca atatggatca gctgtcaaag cagtcatttt aaactatcct
     8521 aacaatccta ctgggacgac attgtctgca aaacaattga aagcaatcgc tgaggtttta
     8581 aaaaaatatc cggtatttgt tataagtgat gaagtatatg ctgaattgac ttatagtgga
     8641 acgcatatgt ccatagctac ttatcttcca gaacaaacaa tcgtgataag cgggttatcc
     8701 aagtcacatg cgatgactgg ttggcgagtg ggctttattt ttgctcaaaa agcccttatt
     8761 gacgaactaa ttaaagtcca tcaatatctg gtgactgccg ctacaacgat gtcgcaaaaa
     8821 gctgcggctg aagcgctaat aaatggggtg aaagatagcg agaatatgaa agagcaatat
     8881 caaacacgaa gagactatct ggtccagcaa ttggcgcctt taggatttga agtgacccag
     8941 ccaaacggtg cattttactt gttttgcaaa ctcccagctg ccattcaaac tgactcttgg
     9001 cagttttgtg tagaattagc ggaacaggca aaagttgcgt gtatcccagg aattgcattt
     9061 ggcccagaag gagaaggtta tatccggatt tcttatgcta gcagcatgga aaaattacat
     9121 gaggcctgca aacggatcaa agacttttta acagaacagt gattcataga ttttatcaaa
     9181 aaaacaatcg ttaattttgc ggtatattct gcaaggagac gattgttttt taaatttagt
     9241 tcaaatgaac gcgtttcttt tgattcataa ctaatgtaat gttaagttta aattgtttag
     9301 aatgatttta aaagaatatt caaggaggtt tcatcaatgg aaaaaatcaa agttgtcatt
     9361 gtaggtgcat ctcatggagg gcatcaatcg atcttggaac tattgacacg atacggtgaa
     9421 aatgtagata tcacgctatt tgaagcagga gactatattt ccttcatgtc atgtggtatg
     9481 gagctgtatt tagaagacaa ggtaacagat gtaaatgatg tacgtaattt cagaccagaa
     9541 aactttccgc aatccaatgt tcatatttta aataatcacg aagtaaaaac gatcgatgcc
     9601 gataaaaaaa cagtaacggt tactagcgca tcagataacg aaacaaaaga atatccttat
     9661 gacaaattga ttttgagttc tggtgtcaaa ccaaacaatc tgccagtacc aggagcagat
     9721 ctagaaaatg tctacttgat gcgtggatac aattgggcaa ccaaaatcaa agaaaaatta
     9781 aatgatccgg ctattaaaaa aatcgctgtc gtaggtgcgg gttacattgg gatcgaagca
     9841 gcagaagcaa gccgaaaagc tgggaaagaa gtcgtattgc ttgatgtgat cgatagacct
     9901 ttaggcactt atttagatgc tgagatgaca gatattttag aacaacacct aaatgaaaac
     9961 ggtattgaag ttttgacaaa tgcaaaaatc gaagctttta ctggtaacgg aaaagttgag
    10021 gcaatcaaaa caagtgaaaa agaaatccca gcagatctag tcattcaagc tgccggtgtg
    10081 aaggcaaata cagaatggtt aaaagggatc gtcgacttag atgaacgtgg ctggatccaa
    10141 acaaatgaat acttgcaaac gaatcttcca gatgtgtatg cagtaggaga tgctacactt
    10201 gcttattcta tcccagcacg caaaaaaatg ccgatcgctt tagcgactgt tgcacgtaga
    10261 gaagcacgtt atgtagtaaa acatcttttt gaagaagtac caaacaaacc gttcggcgga
    10321 gtcgtaggtt cctctgcatt aagtgtcttt gactatcatt ttgcagcaag cggattgaat
    10381 agctttacag cagcaaaagc aggagtagat gtacgagtgt cttattatga agatacgtta
    10441 cgaccaaaat atgtaccaga agaacaagga aatccaaaag tgtccatcca attattctcg
    10501 gatccaatga cccatcaatt gctaggagga gcagtattat ctacctatga tgttacaggt
    10561 caaggaaatg tattggcact agcgatccaa aacaaactga ctttagaaga actagcagaa
    10621 gctgacttct tcttccagcc aggctttgac cgacaatgga gcatgttgaa tctagccgct
    10681 caacaagcgt taggagaaga accatttgta gaataagaaa acgaaatatt tgaaaagagg
    10741 ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
    10801 gtttttaact tggctggact tattaccgca gatgcaagct tttgaacacc gtttattttc
    10861 aaaaaaggga ctgtgacacg atttgtgtac cgacccccaa aagttagacc aaaaaatcta
    10921 acttttggag gtcggtattt tttatggtta aatatacttt tgaacttaaa ttaaagatcg
    10981 ttcatgatta tttggatggc aaaggcgatt ggaccgtaaa aatccagatc aagagattga
    11041 agcggaaatg cttaaaattc gggagaagca caaggattat ggctgtttac ggatgaccaa
    11101 tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa gttcagcgct tgatcaagaa
    11161 gctaggaata aaagtaacgg catacacgcg taaatcacgt cgctacaatt cttatcgagg
    11221 aaaagttgga gcagtagcca aaaatcggat ccatcgtcgt ttctacacca gtatctgcca
    11281 tcagaaaatc acgacggata
//
LOCUS       DAGCXG010000084        11194 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGCXG010000084 DAGCXG010000000
VERSION     DAGCXG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.087"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(85..2094)
                     /gene="metG"
                     /locus_tag="MB022_002136"
     CDS             complement(85..2094)
                     /gene="metG"
                     /locus_tag="MB022_002136"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBT4467116.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2362..2640
                     /locus_tag="MB022_002137"
     CDS             2362..2640
                     /locus_tag="MB022_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467117.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2737..3558
                     /locus_tag="MB022_002138"
     CDS             2737..3558
                     /locus_tag="MB022_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4467118.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3843..4853)
                     /locus_tag="MB022_002139"
     CDS             complement(3843..4853)
                     /locus_tag="MB022_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467119.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4865..5818)
                     /locus_tag="MB022_002140"
     CDS             complement(4865..5818)
                     /locus_tag="MB022_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4467120.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5808..6878)
                     /locus_tag="MB022_002141"
     CDS             complement(5808..6878)
                     /locus_tag="MB022_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467121.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6884..7927)
                     /locus_tag="MB022_002142"
     CDS             complement(6884..7927)
                     /locus_tag="MB022_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467122.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7928..8869)
                     /locus_tag="MB022_002143"
     CDS             complement(7928..8869)
                     /locus_tag="MB022_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467123.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8953..9300
                     /locus_tag="MB022_002144"
     CDS             8953..9300
                     /locus_tag="MB022_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBT4467124.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9370..11031)
                     /locus_tag="MB022_002145"
     CDS             complement(9370..11031)
                     /locus_tag="MB022_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4467125.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3151 a         2278 c         2044 g         3721 t
ORIGIN      
        1 ataagaagga atttacgaaa attgcttctc aaatttcgga aaaagggggt atgacacaac
       61 tagtgtcata ccccctttga atttctatgc gatttctgag ccatttggca tggattttgg
      121 tgcttcgatt acttgtaaag agccatctgg tgcttctgca gaaaggatca ttccttgact
      181 gatttgtcca cgcatctttc gtggtttcag atttgctaca atgacgactt ttttcccgat
      241 caattcgctt ggatcaggat aaaattcagc gattcctgac aagatttgac ggtcttggct
      301 gtctccagca tctagtcgga attgcagcaa cttatctgcc ccttctactt tttgacagtt
      361 gatgacttct gctactttta gttctacttt ttcaaataca tcaaatttga tttgtttttc
      421 tttcgttgaa acaagttctg tttcttctgg atcccatttg actgtatctt cgtttgtttg
      481 agtaccttca gacatttttt cttgaataaa agcaacttct tcttccatat ctaaacgtgg
      541 gaagattggt gtaccttttg caactacttt tgttcctgaa ggaaattcgc caaagtgtaa
      601 tccttcaaga ttcattgttt cagagtccaa tcctagttga ttgaagatct tctctggcgt
      661 ttctgtcatg attggctgaa gtaatatcgc tgcgatacgc aaagattcag ctaaatggat
      721 catgacacta ttcaattcag ctttcttttc ttcgtctttt gctaataccc atggttctgt
      781 ttcatcaatg tatttgtttg cacgagaaat cagcacccag atttcagcca atgctgtatt
      841 gaattccatt ttttccattg catcatggta acgtccgaca acgttagcag ccgtcgtaga
      901 cagttcacta tcaaatggag tgacaagtga ggcatagtca ggaacgagtc catcacaata
      961 tttgttgatc atagcaatcg ttcgattcaa taagttccct agatcatttg ccagatcata
     1021 attgacacgg gaaacaaaat cttctggcgt aaagacacca tctgacccaa acggaacagc
     1081 acgaagcaaa taataacgga gtgcatctaa accataacgt tccacaagca tttcaggata
     1141 aacgacattt ccttttgatt tagacatttt tccgtctttc atcaatagcc agccatgccc
     1201 aaatactttt ttaggtagag gcaaatcaag ggccatcagc atgattggcc aatagatcgt
     1261 atgaaagcga acgatttctt taccgaccat ctgaacatct gcaggccagt atttttcgaa
     1321 tagactttca tcttcggagc catatcctag tgcagtaata taattggata atgcatcgat
     1381 ccatacatac accacatgtt tcggatcact cttgactggt actccccaag taaaagttgt
     1441 tcgtgaaact gcaagatcct ctaatcctgg tttgataaag ttgttgatca tttcgttttt
     1501 acgagattct ggctggatga attcgggatg ttcttcatag tattccaata gacggtctgc
     1561 gtatttgctc atgcggaaga agtatgattc ttcttttacc aattctactt cgtgtccact
     1621 tggcgcttta ccaccgatca cgttaccttc gtcatctttg tacacttctg caagttgtgt
     1681 ttctgtaaag aattcttcgt cagaaacaga gtaccagcct tcatattcac ctaagtaaat
     1741 atctccctgt tctaataaac gatcaaatat tttttgaaca gctgctttat ggtaatcatc
     1801 tgtcgtgcga atgaatttat cataactgat atcaagcgtt ttccataatt tcttgacatc
     1861 agccgccatt ttatctacat attcttgcgg ttttacacct agttcttctg cttttgtttc
     1921 gattttttga ccatgttcat ccactccagt gagataaaac acatcaaagc ccatcaaccg
     1981 tttataacga gcaattgcgt cacatgcaat cgtagtatac gagttgccga tatgaagttt
     2041 gccgcttgga taatagattg gcgtagtgat ataaaaagtt tccttttctg ccacggggct
     2101 cttcctcctt tatcgcatga tgcgaattcg tttccttgct agtataccat aaaaaaagca
     2161 tgctgttagc tttttttcaa aatgaaaaag actggataaa cgacttctat ctacgttcaa
     2221 tctatccttt aaaaggatga ataggttgaa taaatgcggg tttttgtttt gtttcatatc
     2281 tactaaaaaa cggcttttta tttctcccct cacacgtggt ataataaaga ctatctatct
     2341 tagtaaggga ctggaacaat catgcagaag aaacatcttt tttttacact tagtattgct
     2401 tttctttcat tagcacattt aatattcagc tatttttata ttcgtatgta tggttatttt
     2461 aacctccatg ggcatttgaa cagttttatg acagcagctt ggattttgcg ttttatcatt
     2521 gacgtatata tcgttatttg tggcttcttt gccattcgtg aagagcggta taaagtattg
     2581 ccgttttatc tgcttttctt tttattcaat ctcatacttc catttatttt tcatatttaa
     2641 atgataacct agatttttca agtaaatcct tggttttttt tcagagtctg ctacaataag
     2701 gtaaaaagta cttttactgt caatggaggt ggtcagatga accgttttgc ttctaaagaa
     2761 gaagaaaaat tatattatga acataaagca agtgaagaag aatttcttaa ctggtatcaa
     2821 aaacaggaac gtccagaata tgacaagccc tctttgactg tcgatatcgt actgatgtgc
     2881 tataacaaag aagaagatca gctgaaagtt ttgttgatcc aaagaaaagg gcatccttat
     2941 cgaaattcat gggcattgcc tggtggcttt gtccaaaagg atgagtcaac tggagaaagc
     3001 gtcttaagag aaacaaagga agaaactggt gtggttattt caaaagaaaa tattgagcaa
     3061 ctgcatacat tcagtacgcc taatcgagat ccgcgaggct gggtcgtcac agtcagctac
     3121 ttagccttta ttggagaaga accgctaatc gccggagatg atgccaagga agtacgctgg
     3181 ttcacactag aacgtcatag taacaagatc catttatcta gcggagaagt ggctatctcg
     3241 cttgatttag tcactggaga gtcttctggc aaagatactt tggcttttga ccatagtcaa
     3301 atcatcttga aagcattcaa ccgtgtagtc aataaaatgg aacacgagcc gcaagttcta
     3361 caagttttag gtaaagattt cacgattacg gaagctcgaa aagtatttgc taaattctta
     3421 gggatcgatt ataaaacgat cgatcattcc aattttaaga aagctatgct ccaatatttc
     3481 gatgagatcg gtgaacgtcc tgtaggaatc ggccgtcctt caaaaatcta tcagctcaag
     3541 ccggggcacc atgaataagg ttgtgagcaa aagattcaga cctaatcatt caagaaaaaa
     3601 cgcaaaaaca actctttgtg agttacgttt tttgtttcag aattttgatg tattcgtaaa
     3661 ctttctgaat aaaaaattaa caatagcaga aaaagctcgt acgaactttt tctgctattg
     3721 ttttattggt ttgtcataat tagtgagctt ttcttcaaaa gaaaaattcc tagaaacaaa
     3781 agtcattaag actttttatt aacaaggcta accctaacgc tcctatcagt ttcattcatt
     3841 ttttatccct tgataaccga atgtctgccc attttttcta ctacttggct ctttcttacg
     3901 gaattgcttt cttctgctcc gtcggtattg atcaaaagaa attttttatt ataccattgg
     3961 gacttataac gcaattttgt tattaattta tgccagtcgt gaatagaaaa atcagcatgt
     4021 gcctcttctt ttggaaaaga ttctacaaat aagtagtcat attgtttgac caatttccca
     4081 gataattgtt caagataatc ttctcgctgg ttcttttgtt tcagataaag atccattact
     4141 tttctctttt gtttctgata attgcttgcc tgatctaacc tgatctttct atggtgagcg
     4201 gagcggactt tcacattcaa tttccgctgt gcatgttgca gcttttcttc tgtctggtga
     4261 aattttggta aagttacttc tagaggtcga gatgtttgga tcaatgtctt cggatgatag
     4321 gcgatcccga tccatttagc taaagattct ttttttactg gcgaaacatc acaaagtagt
     4381 gcgacataaa attcttcatt atatcgagca gaaatcgtag cggagcgaat cgatccttca
     4441 actggccgat gctgatttat cttgattagt tctttttgtt ttggtaactt taagtagcca
     4501 ttttccagat agatcgtacg ggtttggttg ttggtcgtat aagattgcca catacttttt
     4561 ttcgttttaa gctttgggaa gctggcacgt cccttgaaat aattgcgaaa agcccgatcc
     4621 aaattaagct gtgcatttgc taaagcaaga ctgtctactt cttttaagaa tgggtattct
     4681 ttttttaatg tagcgggtgt gagcgaaaaa ttggctactc ctgtttgttg ccaacttttc
     4741 tgccgctttt tcagcaaatg gttataggta aatcgagcac atccaaaagt acgaatcaat
     4801 tgctgttttt gttcttctgt cggatacata cggaatttaa aagcttttag catctatgtc
     4861 cctcctagtt agcaaccaga agagattcct tttgtcgttt tagttttttt gcgtaatatg
     4921 ggacttcctg ctcagttgca tagacaaaat gttctggtgc gatttcacga agttgacgta
     4981 cttgtccgtc atctggttca ggttgatatt tgattcttct gcgttttctc tcatggtctg
     5041 gatctggtag aggaatagca gataacaaac tttctgtata aggatgaaca ccgtgattat
     5101 agatttcatc acttgttccc atctctaata acaagccgtt atgcattaca ccgattcggt
     5161 cactaatatg tttaaccatc gacaaatcat gtgcaataaa tagataggtc aattgatgtt
     5221 ctttttggag atcttgcaac aaattgacca cttgggcttg gatcgacaca tctagtgcag
     5281 aaattggttc atcacaaatg ataaaacgcg gttttactgc caatgctcgt gcaataccaa
     5341 tacgctggcg ttgcccacct gaaaattcat gaggataacg agtaccgtgg cttggattca
     5401 atccaacagt tctcaataga tcatcgactt tttttgcacg ctcttcagat gatttagcta
     5461 gtccattaac atcaattcct tcggcaatga tatctctgac tttcatacga ggattcaacg
     5521 aagcgtatgg atcttgaaag atcatctgaa catcatgccg gaatttcgtc aaaccttttt
     5581 tatctttgat cttcgttata tcttgaccgt cgaataatat ctcaccatca gtaggttcat
     5641 tcaagcgaat gatcgtacgg ccagtagtgg attttccgct tccagattct ccaactaaac
     5701 cgaatgtttc tccttcataa atgtgaaagc tgatgtcatt tacagcatgt acttcattct
     5761 tttttcctac attgaaatac tgtttcaatc ctttaacttc aagtagttta tttggcatca
     5821 gcaattcctc cttgaccgta agtatgtttt tgtttctctt taaagatagc ccagcgacgt
     5881 tggatttctt ctggcggtgt cactttgggt gcctgcggag caagtagcca agttgctgcc
     5941 ttgtgtgttt ttgatagttc aaaaaatggt ggttcttgtt ctgcatcgat tttcatcgcg
     6001 aattcatttc ttggatagaa tgcgtctcct tttggcgggt tcaataaatc cggtggagaa
     6061 ccaggaatag catacaatcg atcgttcgca ctatctaatg tcggcattga accaagcagt
     6121 ccccaagtat agggatgttg cgggttgtag aaaatttctt ccgatgttcc aacttcaacg
     6181 attcttcctc catacatgac agcgacacga tcagcgacat tcgctactac accaaggtca
     6241 tgagtgataa agataattga tgtatcgatt tttgtctgaa tttctttcag cagctccaaa
     6301 atctgtgctt gaatagtcac atcaagcgct gttgtaggtt catcagcaat caggatttcc
     6361 ggataacata ccagtgcaat cgcgatcacg atccgttgac gctgacctcc tgagaactga
     6421 tgcggataac tgttgatacg tttctcagct tcgggtattc caactaattt aagcaattct
     6481 aatgcagctt tttgtccctc tttttttgaa actttattat gctttctaag ggactcagcg
     6541 acttgcttcc caatgggcat cgtcggatct aaagaggtca ttggatcttg gaaaatcatc
     6601 gctatttctt taccacggat tttttgcatc tctctttctg attttccaac gatatcgtta
     6661 cctttaaaga ggatttgacc agcatctata ttggcgttgc ttgacagcaa gcgcatgatg
     6721 cttctggtcg tcactgattt tccacttcca gattccccaa cgatcgccaa tgtttctcct
     6781 ttattcaaat gaaaattgac accgcgaatc gctcgtacct ttccagcata cgtatcaaaa
     6841 gagatttcta agtctttcac ttctaaaatt ttcgtcatcg tcttcactct ttcattttag
     6901 gatcaaaggc atcacgcaat ccatcagcaa gtaagttaaa gccaatcatg ataaccgata
     6961 aagttactgc cggaatccat aatagataag gcagatacag gaatgtcttg tacccttcgc
     7021 tcaacatcgt tcctaatgaa gctgacggag gcgtaagacc taaaccgata aagcttaaaa
     7081 aggcttcgaa gaaaatagca gatggaatac taaacatcat ttgaataata atgacactag
     7141 agatattcgg caaaatatgt ttgaatgcaa ttttcattct tgattcacct aatgtcaatc
     7201 cagctaaaac atattcttga tcttttaatt tcattgtttg tgcacgaacg atacgtgcca
     7261 ttggtatcca gtttgtgatg gccatagcag ccacgattga aaaaattcct ggttcaaaaa
     7321 cgaccaacat caaaatcatg acaacgaggt ttggaattcc tgataagatt tccaaaaaac
     7381 gttgcatcac gttatcgaca cgtccgccta acaaaccaga aaccaacccg taagctacac
     7441 caatcgttac atctagtaat gcagcaataa atgcgatcag caacgagatt ctcattccca
     7501 taaataagcg gcttaataca tcacgtccca aaccgtctgt tcctaaaaag aaatttacgt
     7561 ttgaagggac gtttgcttga gcatatttat caacaagttc accaccaacc attgcttttc
     7621 cattcaatcc atcgatattg attcccggaa tacgaggagg caaattgata taagccacgt
     7681 tttgggcagt tggatcatga ggagaaacaa agattgtgat cacagagatc aaaatgatga
     7741 ccaatagtaa aaccattgaa ataacggcag ctttgttttt ctttaaacgg cgccatgaat
     7801 cttggataaa attcaaagaa ggtgcagtaa tctgttcacg ttcttcttcg gtattttttt
     7861 gtaatggaag aaattcatcc gcagggatat ctgcaactgt ttcataacgc tttggtatca
     7921 tttccattta tcctcggctc ccttctgaaa cacgaattct tggatcaact agaccgtaca
     7981 atagatccac gatcaaaata acaaaaatca gcataaatga gtaaaggatc gttacagcca
     8041 tgatcgtagg ataatcattt gttgtgattg acttaacgaa ttgttccccg ataccaggaa
     8101 tagcaaagat attttctacc accagtgatc cggtcatcaa tgctacagct aacggtccta
     8161 ataaagtcat caatggaatc aaactatttc gaagaccatg tttaaaggcg atttcccaac
     8221 ggctcaatcc ttttgctttt gctaattcca cataatcact atgcagtact tccaccattt
     8281 ccgtacggat aaaccgagca gagtcagcaa gcggcgacat ggccaacgca attgttggta
     8341 agattgtgta cacaaagcca tcccattttg cgattggcaa aacttgtaat ttgattgcaa
     8401 agatatattg taataaaaca gcaaatacaa agtttggaat ggaacgacct aatatcgcta
     8461 cgagtgtaga agatgtatct gcccatgtat tttgcttcat ggcagagatc gttcctaaaa
     8521 tcgtccctag gactgttcca aagataattg cctgtaaccc taactgcaag gatggtccga
     8581 ttcttccagc taacaaatgt gcgactggct ggtttttaaa ttggaaggaa attccaaaat
     8641 ctccttgaag caaattagaa agataaatcc catattgtac gataactggt ttatccaaac
     8701 cgacttgttt atttagcatc tcgattgttt caggactcaa acgttcctga ttcgtatatg
     8761 gcgtgcccgg cagcaattgc atcaagaaaa aggtgattgt tgcaattaac caaagcgtga
     8821 tgatcatgaa aaagacacgt tttaatacat atttgatata actattcact aatgtttccc
     8881 cctagctttt gacttctgac agatgtttcg ctacattatt agataatgaa ccaaaaataa
     8941 aggagcaatc aaatgaaaaa aaaatctatc ccctacgcag tagcttttct actgattctg
     9001 gttattttaa taaaaaatct catcaaccat tcctttacac tcattcaatt atctaatgat
     9061 ctattcttat ggtcattgcc gtttctgatt atcggcggtt ttttatgggt cttttcttcg
     9121 ggtttcttcg atcattttca gcgatcggtc catcttgcac gaacaaggaa ccgaaagaaa
     9181 aaaccagaat tttcctcgct ctcgtctgcc agctacggta tgtacagctt ttggctgatc
     9241 attgccggaa tactaattgc tctttctgct attttcatgc ttttttcttt gttaggataa
     9301 gtgaatatga gaacgaggtt gcgacatttg ccgcagcctc gttttttctc atgctcacgg
     9361 tattaacgat tattcttcaa cgtatgtcca tttgtaatcc catgaagcgc ctgccgcatg
     9421 gtgaacgata cctttgattt tttcattcac taggtgacct tctgcttttt ggtacaccgg
     9481 aacgacaccc atatcatcag agatgatctt gattgctttt tggaaatcat cccaacgggc
     9541 ttgttcatcg ccagcgtctg ttgatgaagc tgctttaact gcttcgtcat attctgcatt
     9601 gctccagcga ccacggttgt atgaattacc tgtgacaaac aaatcagtaa agctgcttgg
     9661 gtctgcatag tcagcagccc atccgcctag tactgcgtca aaatcacccg aagttgagcg
     9721 gtcaagacgt acagagaatg gaacaggtgt tggtttgact gttacgccat ccaagttttc
     9781 ttggattgag ttttgaacat attcgattac ttttttcgat gaatcatcat cagaagccat
     9841 caaatcaaat tctaaagaat caacgcccaa ttcttttttc gctttttccc aatattcttt
     9901 tgctttatct ttgtcataag aaactaattt tccagcttct tttgcaaagt cttcgttcgt
     9961 tgttggactt tttaccatat ctgatgggat caatcctgta gagacaactg atccgtcacc
    10021 tagtacttgt tttactaaag cttcacgatc gatagaataa gaaatagctt ttcttaggtt
    10081 cacattgttg aatggtgaat tcttttcacg ttgattgaat tcgatatagc ttgtccgcgc
    10141 ttctttaaca gaagtataag ctggatcgtt tgcgttttgc tgtgctaact caccagacaa
    10201 gatcacgtca tctgcttggc catctttgaa taagttcaat gcagtagaag attcttttac
    10261 tacactgact ttgatttcag aaagttttac tgtatctttg tcccagtaat gatcattttt
    10321 cttataagtc cattctgtat ctgttcctgg tccatcaaaa tcttctaatg tgaatggtcc
    10381 attataaaca gctttatcac ttgcagatgc ataggcatcc ccattttctt ccactacgga
    10441 ttgtttttgt gggaaaaatg atgggaaagc aagcagatat tggaaatatg gtgtttgttt
    10501 cgtcaatttg atttctaatt catagtcacc aactgctttg atccctagtt ctgatttatc
    10561 tttctttcca gctgtaatgt cttctgcatt ttcaactggc gcatacaggt aagcatattc
    10621 agaagctgta gctgggtcta ctgttcgttg ccagccataa acgtaatccg ctgctgtaac
    10681 aggatcacca tttgaccatt ttgcgtcttc tcgtaatttg attttgtaag ttaatccgtc
    10741 ttcactgact tctgccaact cgctagctcc tgccggctgt ggtttgctgt cttcatcaag
    10801 acggtaaatt ccttcataaa cgttattcaa tgctgtaaaa ctgattgtat ctgttgcaac
    10861 tgataagtca gctgtaggca tttcttgagg cacgactaag ttaaatacgc ctcctccatc
    10921 tgctgaaccg ctttttgctg aactactgct tgtggctgaa ctgccgccat aacatccagc
    10981 aagtacaagg ccgcataccg caaccaaacc aaatgtaaat ttctttttca ttttacgtac
    11041 ccctttattg gaattttcag aataattaat attttctgaa gttgtttaag agtttaatga
    11101 ataattatta aaaaatcaac actttttttt gtctttttca taaaataatg attttttcgt
    11161 gaggtttcaa ggaatttttc agaaaaaata gctt
//
LOCUS       DAGCXG010000085        11186 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGCXG010000085 DAGCXG010000000
VERSION     DAGCXG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.088"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            6..305
                     /locus_tag="MB022_002146"
     CDS             6..305
                     /locus_tag="MB022_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4467126.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="MB022_002147"
     CDS             378..1739
                     /locus_tag="MB022_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBT4467127.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="MB022_002148"
     CDS             1761..2021
                     /locus_tag="MB022_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467128.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="MB022_002149"
     CDS             2035..2787
                     /locus_tag="MB022_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBT4467129.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="MB022_002150"
     CDS             complement(2881..3486)
                     /locus_tag="MB022_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4467130.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="MB022_002151"
     CDS             complement(3539..4513)
                     /locus_tag="MB022_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBT4467131.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="MB022_002152"
     CDS             complement(4891..5610)
                     /locus_tag="MB022_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4467132.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB022_002153"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB022_002153"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4467133.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="MB022_002154"
     CDS             7161..8558
                     /locus_tag="MB022_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4467134.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="MB022_002155"
     CDS             8639..9079
                     /locus_tag="MB022_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4467135.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="MB022_002156"
     CDS             9227..9553
                     /locus_tag="MB022_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4467136.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="MB022_002157"
     CDS             9554..10303
                     /locus_tag="MB022_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467137.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="MB022_002158"
     CDS             complement(10383..10838)
                     /locus_tag="MB022_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4467138.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3551 a         1958 c         2295 g         3382 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgtttt
//
LOCUS       DAGCXG010000086        10835 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGCXG010000086 DAGCXG010000000
VERSION     DAGCXG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.089"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(164..388)
                     /locus_tag="MB022_002159"
     CDS             complement(164..388)
                     /locus_tag="MB022_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBT4467139.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="MB022_002160"
     CDS             complement(586..2166)
                     /locus_tag="MB022_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBT4467140.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="MB022_002161"
     CDS             complement(2567..3943)
                     /locus_tag="MB022_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4467141.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="MB022_002162"
     CDS             complement(4071..5189)
                     /locus_tag="MB022_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4467142.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="MB022_002163"
     CDS             5331..5765
                     /locus_tag="MB022_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467143.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="MB022_002164"
     CDS             complement(5843..6376)
                     /locus_tag="MB022_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBT4467144.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="MB022_002165"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="MB022_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBT4467145.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="MB022_002166"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="MB022_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBT4467146.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="MB022_002167"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="MB022_002167"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4467147.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="MB022_002168"
     CDS             complement(10101..10679)
                     /locus_tag="MB022_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBT4467148.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         3187 a         2205 c         1858 g         3585 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaaca
//
LOCUS       DAGCXG010000087        10666 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGCXG010000087 DAGCXG010000000
VERSION     DAGCXG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.090"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            79..561
                     /locus_tag="MB022_002169"
     CDS             79..561
                     /locus_tag="MB022_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467149.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            758..1648
                     /locus_tag="MB022_002170"
     CDS             758..1648
                     /locus_tag="MB022_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBT4467150.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1974..4448
                     /locus_tag="MB022_002171"
     CDS             1974..4448
                     /locus_tag="MB022_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4467151.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4551..6287)
                     /locus_tag="MB022_002172"
     CDS             complement(4551..6287)
                     /locus_tag="MB022_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBT4467152.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISNYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDANSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6284..6988)
                     /locus_tag="MB022_002173"
     CDS             complement(6284..6988)
                     /locus_tag="MB022_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4467153.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7119..7622
                     /locus_tag="MB022_002174"
     CDS             7119..7622
                     /locus_tag="MB022_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBT4467154.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7582..7920
                     /locus_tag="MB022_002175"
     CDS             7582..7920
                     /locus_tag="MB022_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBT4467155.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7941..9359
                     /gene="ffh"
                     /locus_tag="MB022_002176"
     CDS             7941..9359
                     /gene="ffh"
                     /locus_tag="MB022_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBT4467156.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9471..10049
                     /locus_tag="MB022_002177"
     CDS             9471..10049
                     /locus_tag="MB022_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBT4467157.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10001..10573
                     /locus_tag="MB022_002178"
     CDS             10001..10573
                     /locus_tag="MB022_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4467158.1"
                     /translation="MFPQRRGRTYRQKGRIKHAKIIFYGAISLDGYLATKTDDLQWLF
                     DTPTGKQTTYEAFYETIDTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPD
                     AEVVNEDPVAFLKNLQMTEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIP
                     LFQKGTYEARFEMLDTTRFGEFIELHYVRK"
BASE COUNT         3477 a         1927 c         2242 g         3020 t
ORIGIN      
        1 aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag
       61 tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt
      121 tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg
      181 gagatattag gcctgatcca aggaagcgga ttagtggttt ctaggaattt tccagctttg
      241 actgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac
      301 tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg
      361 ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggattcagct
      421 tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt
      481 attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta
      541 tctataaaaa ataactattg atggtggatc atcagtaaaa aagcatatga gctgcgtaaa
      601 atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat
      661 gatttttaca taacttaact atatttttac agcaaccata cgagggattt acagtcgact
      721 gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct
      781 aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc
      841 tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc
      901 gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa
      961 ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg
     1021 agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc
     1081 aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac
     1141 agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa
     1201 ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag
     1261 cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca
     1321 agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag
     1381 ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga
     1441 accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta
     1501 cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga
     1561 aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga
     1621 acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac
     1681 tattaaggaa aaatcaacaa tgtttcccca catatacatt tttcctttaa ttgtgaaaaa
     1741 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     1801 tttattcgaa aaaaagagct acgacataga actgtcgtag cttttttttt gctatatgac
     1861 aggaatgaaa ggtgtttgca agcttaatgt gaggaacact ttccatatct gttctatact
     1921 ggataggaga agtttggaaa tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga
     1981 ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca aagatcatca gataaagtca
     2041 tccgtctttt tccatcgtat aaagaaggat taacggcaga tcaagtcaag gaacgaatag
     2101 aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa aacaaatcag caaatcgtat
     2161 tggaaaacat atttacgtat ttcaatttga tctttcttat cttagccatt ttgctttgtt
     2221 tagtagaatc ctataagaac ttgacttttt taccagttat catagccaat acaggaatcg
     2281 ccatttttca ggaaattcga tccaaaaaga tcttggacga actaaatgtt ttaaatacag
     2341 caaaagtcaa tgtcatacga gacgggatag aacaaacaat agatatagaa gaccttgtaa
     2401 tagatgacat tgttatttta gagactggac atcaaattcc agcagatgca gaggtcgtgg
     2461 aagggaaact gcaagttaat gaggctttat tgacaggcga agcagatgaa ataaccaaag
     2521 aaataggcga tccattaatg tccggcagtt tcattgtttc cggaaaagct tacgcccgat
     2581 tagacaaagt tggagcagat tcgtatattt cccagctgac agtcaaagcc aagacaatgg
     2641 gagaaggaga acagtcggag atgattgctt ctcttaatca attgatcaaa tggattggga
     2701 tcatcattat tcctatcggt atcctgcttt tttctcaaag ttactttttt aatggaaaca
     2761 cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat cggtatgata cctgaaggac
     2821 tgtacttgct gacgacgatt gcattagcat taagtgcgac taaactagca aagcaaagag
     2881 ttcttttaca caatatgaaa agcattgaga cattggcaag ggtaaatgtc ctttgtgtag
     2941 ataaaacagg aacgatcaca gaaaataaaa tgtctgttca aaaagtgatc gtctctaaaa
     3001 aacaagaaat aatcagtcag tcagatcaat tggaagcatg cattgctgac tatgcaaaag
     3061 cgatggcagg agacaatgca acaatggaag ctgttaaagc atattttaca aaaaccactg
     3121 atcaatccta tcaccatgtt attccttttt cttctgtcca aaaattcagc agtgtagcgt
     3181 tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg
     3241 aatatgcgga agagtttgtt tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg
     3301 tgtacgatgg gattcttgaa gaaccagtat tgacagaagc agtcacacca ttaggctata
     3361 ttctgattgc taatcctatc agaaaagaag caagagccac atttgattac ttcaaaaaac
     3421 agcaagtagc catcaaagtg atttctggag ataatccgct caccgtatcc aatgtagctg
     3481 tccaagcagg gatttcacat gctgatcagt acgttgatgc atctaaatta tcagaagaag
     3541 aatatgaagc agctgtcgaa aaatatacag tttttggacg cgtcaagcca gaacaaaaga
     3601 aaatcttcgt tcaattgcta aagcgtcgta acaatacagt agctatgaca ggtgatggcg
     3661 tgaatgatat tctagccatg aaagaagcag actgcagtat agcgatggct tccggaaatg
     3721 aagccgccat gcaggcctca caagtggtct tacttgattc tgacttttca cgcatgccag
     3781 aagttgttgc agaaggacgg cgcgttgtga acaatattga gcgatcagcc agcttgtttt
     3841 tagtgaagaa tattttctcc tttttgctct cactgttttc agtgatcttt gctctgacct
     3901 atcctttaga accatcacaa atcacattga ttagtttgtt tactatcgga cttccatctt
     3961 tcttgcttgc tctagaagaa aacaaaaagc gaatcagagg gaaattcatc atgaatgtga
     4021 tggaaaaagc tgtgccaggt ggactaacag atatgatcgt agtcggtgcg ttggtgatct
     4081 gcggggtgac tttgaatcta aataaaacag atgtttctac agcatctacc atgcttttga
     4141 ttgcagtagg gttcctcgtt ctctataaaa tctgttcacc gctgaataaa ttcagaagtc
     4201 agatcattct tttctgtgct agcggtaact ttttttcggt catctttttg cataagctgt
     4261 tttcaatcac cggtatctca gctgtatcac ttttgctgtt gagcattcta tttttctcgg
     4321 cagattcgat ttttaggcat ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg
     4381 aaaaagagca aaagccgttt agtcgcgtag ttaaaaaatt tctatcgata tttaaacgag
     4441 aaaattgacg aaaagtatct caaaaaagag taaaaaagaa caaccactcg tgtatctttc
     4501 gaagcaggat gagtcatcca actttaaaac gcacgagcag ttgctctttt ttacattttc
     4561 ggaatggtaa cggtgaaagt cgaacctgtt ccaagatgac tgtctacagt aatcgtacca
     4621 tgaaggagtt gcacgtagtc ttttacaatc gctaacccta atccggtccc gcctgaatgc
     4681 cggcttcgcg ccttgtctac gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg
     4741 tcgataccta taccaaagtc ttggacagaa aacgatagat catctgttgc ctgatagcgg
     4801 ataataatct cgctgtcgct ttttgaatat tggacagcat tttcaatcag atttttgata
     4861 attggataaa ggatatctgt tttagtagta aatggttcgt tctttccaat caatcgaatc
     4921 gtcaaccgtt tcttttcgat gattggctga taattttgaa cgatttgctg gaaataaggt
     4981 tctgtataaa ttgtctggat ctcgtaagaa ttctcgccat cttttgacag ctggatgatt
     5041 tcccgaatca gtttatctaa ccggatcgca tctttttgca tgatctcaag aaaagaagtc
     5101 agagtttgcg ggtcatcttt tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa
     5161 gtcacaggag tttttaattc gtgagagacg ttgccaacga aatccttttg tagcttttct
     5221 aaacgtcgca ctttcgttaa gtcatacgcc acccctagaa tctgtccggt accttctgaa
     5281 ttagtaaagt agcgcaaact caagtctaat gtctgccgac cttctgtaag ggtgatctcc
     5341 tgatggatca gcggatgctc aggagtcact tgatgaatca gctgaatgag ttttggctct
     5401 tgaatgactt ctgtgtaccg ctgttcaggt tgatagctta ccacgtttaa atgtagctgc
     5461 atctttggat taagcagtag taaacgagaa ttagcatcat caatcaggaa tacgccaatc
     5521 atcagttcgt tcagtaacgt atataattgc tcttctgtgg tagtatacgc ccgatacatc
     5581 ttactcattt gttcgctcaa cgcattgatc gtctgataca attcttccca ttgatcgggt
     5641 gtttgcatga ttacttccgc ctgattagga ttttgcacca ttttttttag tacgggcagc
     5701 acggttttca atggctggtt tttttgatag atcagataat agatcattcc tgcaagaaca
     5761 aggaagaaag acagaaagaa gaaaaagacc caacggcgaa aactctcagt tctcggtaaa
     5821 aaaccgcttg tcggttctgc gatacgtaaa atggcttcta actggccact ttttttgact
     5881 ggtaatgcga catataaaag atccccattc aaagtagtac ttttgcgcaa agcgctacct
     5941 aggttcccac cattcaatac ggctttgatt tcgggacggg agtttcgctg ttcattcaat
     6001 gtttcattgt ttgtgtcaaa taagatcttt ccagtagcat ccattaaagt aatccgttcg
     6061 ttcgactgat ggacgaattc ttccaaagca gttttgtttt gcgctgcttg gaggttttcg
     6121 acatctaatt gccggatcag cagttgtgct ttttttgtca ggtagctttc ttgctgttcc
     6181 acgatctgct gttgaaaata attggaaatc aattgccagc caccaaaaaa caaactaact
     6241 aaaagaataa gaaagaagag acgttcataa atttttttcc atttcatcgt tttggttcct
     6301 gaaacttata gccaaatcct ctaatagtca ccaagtactt tggatgtttt ggatctcgct
     6361 cgattttttc tcgcaaatga ctaacgtgta catctacgat tcggctttgt cctgcgaagt
     6421 caaaattcca aatgcggtct agcaatgtgt ctcggtcgat cacacggtct tttcgtttca
     6481 tgaaataaac aagtaattca aattctttcg gtgttagttc aatcagatgg tcgtctacgg
     6541 tcacttgata attggtcaaa tcagcagtga tttgtccaat cgatagatat tcaggctcag
     6601 cttcatcttt tgattgctta cgaggttcaa tccgtctgaa tattgctttc atacgggcta
     6661 atacttcgcg aggactaaat ggtttggtca aatagtcgtc agcacctatt tctaggccaa
     6721 tgatccgatc cacttggtca tctttagccg tcagcatcaa aatcggtgtt tcattttttt
     6781 cttgacgcag ccgctgagtg atctccatgc catccatcgt tggaagcatc acatctaaaa
     6841 tgatgaaatc aaatgattgt tctaatgcta attctaatcc ttcgcctcca tctgtggcac
     6901 tggttacctt gtagccttct ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt
     6961 catcatcaac aactagtatt tttttcattc aaatctcctt taaataggtt tcattctgtt
     7021 tatattttag catagaatca gtacaaaggg ctattttaca aaagaaataa atggtataat
     7081 aggaaggcac tttgctgaat agaaaggaag aacagtcaat gattggaatc agtgcatgtt
     7141 taggcggtct ggcctgtcgc tatgatggac agaaaaaaga agtaccagaa ttgatggaac
     7201 tagttgaaac tggagaagct gtcatggtct gtccagaagt gatcggcgga ttaccaattc
     7261 ctagagatcc cgctgaaatc attggtggaa atggattcga tgtgtggaaa gatcaagcaa
     7321 aagtatggac gacaagcggc gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag
     7381 cctatcaaaa attgcaggaa aaacacattg atctagtcat catgaaggaa aaaagccctt
     7441 catgcggaac ccattcgatt tatgacggga gtttttccgg aataaaaaaa aatggtgccg
     7501 gagtggctac tgcttatttt attcagcaaa aaatgactgt gctctctgat gaagaatggc
     7561 tgaaaactag aggtgagcag aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt
     7621 gaattttatt cgaccttatt gacagaaaaa caaatgaact acatggaatt atattatgca
     7681 gatgattttt cattaggtga gatcgcggaa gaatacgaag taagccgaca agcagtatat
     7741 gacaatatca agcggacaag caagatcttg gaggattatg aaaaaaaact ccatcttttt
     7801 tctaattata ttgttcgaga acaggtattg gaaaatatga ctgcatatat tacagataag
     7861 tacccggaag ataaaaaact acttgaatat gtacagcaaa ttcaagcaat cgaagaataa
     7921 ataaaaggaa tgaagaaaat atggcatttg aaagcttaac tgaacgtctc cagcaggcga
     7981 tgggaaaaat aagaaaaaaa ggaaaagtat cagaagctga cgtaaaagaa atgatgcggg
     8041 aaatccgcct tgccttacta gaagccgatg ttaacttgca agtagtcaaa gattttacta
     8101 agcgcgtacg cgaacgtgct gtaggggcag aagtgctgga aagtttatca cctgcacaac
     8161 aaatcgtaaa aatcgttgat gaagaattga cgatcacact tggttctgaa acagcagagt
     8221 taaataaatc accaaaaata ccaactgtta tcatgatggc tggtcttcaa ggggcaggta
     8281 aaacgacctt tactggtaaa ttagccaatt atttaaagaa aaatgaaaat gctcgtccgc
     8341 tcctaatcgc aggggacgta tatcgaccag ctgcgatcga ccaattgaaa gtattaggtc
     8401 aacagctaga cgttcctgta tttgatatgg gaactgaagt cagcccggta gagatcgtcc
     8461 gtcaagggat ggagttagca aaagaaaaga aaaacgacta tgtattgatc gatacggcag
     8521 gacgtctgca tatcgatgaa acattgatgg atgagttgaa acaaatcaaa gaattgacgc
     8581 aaccaaatga gattttgctt gttgtcgatg ccatgactgg tcaagatgcc gtcaacgtag
     8641 ctgatagctt caaccagcaa ttaggtatta caggggtcgt catcactaaa cttgatgggg
     8701 atactcgagg cggggcagca ctatcgatcc gctcagtgac aggcgcacca atcaaattca
     8761 tcggttctgg tgaaaaattg actgacttag aagtattcca cccagatcgt atggctagcc
     8821 gtatccttgg tatgggggat atgctgacat tgattgaaaa agcgcagcaa gactatgacg
     8881 agaaaaaagc ggaagaactt gcacagaaaa tgcgggaaaa ttcattcgat ttcaatgact
     8941 ttattgaaca gcttgatcaa gtgatgggta tgggcccact ggaagatcta atcaaaatga
     9001 tcccaggaat gaatcaagtc cctggaatcg aaaacgtcaa ggtagatcca aaagatgttg
     9061 aacgcaaaaa agcgatggtt tattctatga cgccagcaga aagagcaaat cctgatctgt
     9121 tgaacccaag ccgtcgtcgc cgaattgctg ccggttctgg aaacagtgtt gttgaagtga
     9181 atcgaatgat caaacaattc aaggaatcaa gaaaaatgat gcagcaaatg tccaaagggg
     9241 atatgaacat cccaggtatg gaccaaatgt ttggttcagg cgtcaaagga aaactaggca
     9301 aaatggcgat gaatcgaatg atcaagaaaa ataagaagaa aaagaaaaaa cggaaataag
     9361 atgaaaaaaa cagcttcggt acagactgaa aatgtctact gccggagctt ttctatcagc
     9421 aatttttatt ctttactaat acaattactc taaacgaaga ggtggcacag atgagatgtc
     9481 cgtggagtga acaaacagaa agtatgcggc aatatcatga tactgtctgg ggtgttcctg
     9541 aatatgatga ccaaaagctg ttccgaaaat tgatgcttga tattaatcaa gccggattga
     9601 gctggcagac gattttaaat aaaagtgaag cattcgatga agcatatgat cgattcgata
     9661 ttaaaaaagt cgcaaattat ggagaaacaa aaatcagtga actaatggaa aacaaaggaa
     9721 ttattcgtaa ccgaagaaaa atcgaagcag ccgtccaaaa cgccaaagca gtcctgaaaa
     9781 ttcaagaaga aatcggcagt ttttcagagt atctttggtc gttttctaat ggaaaaatcg
     9841 ttgattcttc ttatcattca caagaagaag tcccaacaac aagtcctctt tcagatgaat
     9901 tagcaaaaga tctaaagaaa agaggattta aatttatcgg aagcacaaca atctatgctt
     9961 ttttagaagc tgtcgggatc atcaatgatc accttgacag ttgtttccgc aaagaagagg
    10021 tcgtacgtat cggcagaaag ggcgaataaa gcatgcgaaa atcatatttt atggtgctat
    10081 aagtttagat ggctatttgg caacgaaaac ggatgatctt caatggcttt ttgacactcc
    10141 aacaggtaag caaaccactt atgaagcttt ttatgaaaca atcgatacga ccatcatggg
    10201 cagaaaaact tatcaggaag caaaaaaata tttggagacc gaagcaattt atccagagaa
    10261 aaagaatttc gttttttcca cccacgcaca attacaattg ccagatgctg aggtcgtaaa
    10321 cgaagatcct gttgcttttc taaaaaatct acaaatgaca gaaggaaaag atatctgggt
    10381 tgtgggtggc ggtcgattga tcaaaccgct attggaacat caaatgattg atgaatggta
    10441 tatccagatt acccctgtat tattagggga cgggattcct ttattccaaa aaggaacata
    10501 tgaagcgcgt tttgaaatgc tagacacgac acgattcggt gaatttatcg aattgcatta
    10561 tgtacgaaag tagaagtaaa taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac
    10621 atagttgcct tttttattac cgttatgctt tttactctgt taattt
//
LOCUS       DAGCXG010000088        10653 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGCXG010000088 DAGCXG010000000
VERSION     DAGCXG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.091"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="MB022_002179"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="MB022_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4467159.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="MB022_002180"
     CDS             1873..4539
                     /locus_tag="MB022_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4467160.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="MB022_002181"
     CDS             4903..5655
                     /locus_tag="MB022_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4467161.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="MB022_002182"
     CDS             complement(5677..6831)
                     /locus_tag="MB022_002182"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBT4467162.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="MB022_002183"
     CDS             7016..9457
                     /locus_tag="MB022_002183"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBT4467163.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="MB022_002184"
     CDS             complement(9787..10491)
                     /locus_tag="MB022_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467164.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3407 a         1907 c         2237 g         3102 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt ata
//
LOCUS       DAGCXG010000089        10595 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGCXG010000089 DAGCXG010000000
VERSION     DAGCXG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.092"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(1273..1938)
                     /locus_tag="MB022_002185"
     CDS             complement(1273..1938)
                     /locus_tag="MB022_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467165.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="MB022_002186"
     CDS             2118..4100
                     /locus_tag="MB022_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBT4467166.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="MB022_002187"
     CDS             4104..4742
                     /locus_tag="MB022_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467167.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="MB022_002188"
     CDS             4770..5198
                     /locus_tag="MB022_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467168.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="MB022_002189"
     CDS             5655..6566
                     /locus_tag="MB022_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4467169.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7020..7334
                     /locus_tag="MB022_002190"
     CDS             7020..7334
                     /locus_tag="MB022_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4467170.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            7350..7736
                     /locus_tag="MB022_002191"
     CDS             7350..7736
                     /locus_tag="MB022_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4467171.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            7765..9150
                     /locus_tag="MB022_002192"
     CDS             7765..9150
                     /locus_tag="MB022_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4467172.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            9153..9305
                     /locus_tag="MB022_002193"
     CDS             9153..9305
                     /locus_tag="MB022_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4467173.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            9328..10533
                     /locus_tag="MB022_002194"
     CDS             9328..10533
                     /locus_tag="MB022_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4467174.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3596 a         1725 c         2061 g         3213 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttct acaaaaaata gcataaaaat ctagttatcc gcataaaaac
     6721 tggacttatc acactttatc aaggtcaaaa ccactcaatt tactactaat ttactactta
     6781 tgaatgagct ttgatacgac gatttatcct tgaaaagtga agatataaag atacttccaa
     6841 taaaatttga atatttaata ggtagacact tcaaaaaatg aggtgtctat ttttttaccc
     6901 gattttgaaa ggaagtgaac ttatgaaaac aaaaaatcaa gaatcaaaag gtcgttcccc
     6961 actctttaag accatcaaac attcattcag ccaataaaaa agaaaggata ggtaaaaata
     7021 tggaacttaa atttgtgatt cccaacatgg aaaaaacatt cggcaattta gaatttgctg
     7081 gcgaggataa agtcgttcag cgaagaatca acggacggct aactgtctta tcaagaagct
     7141 ataatctcta ttctgatgtt caaagagcag atgatattgt ggtggtgctt cctgctgaag
     7201 ctggcgaaaa acatttcggc tttgaggaac gtgtgaagtt agtcaatcca cgtattaccg
     7261 cagagggcta caaaatcggc actcgtggtt ttacaaatta ccttttacat gctgacgaca
     7321 tgataaaaga ataaagaaag agaggaaaaa tgatgagatt agcaaatggc attgtattag
     7381 ataaagacac gacttttgga gaattgaaat tctctgctct acgtcgtgaa gtgagaatcc
     7441 aaaatgaaga cgggtcggtt tcagatgaaa tcaaggaacg tacctatgac ttaaaatcca
     7501 aaggacaagg acgcatgatt caagtaagta ttcctgccag cgtgcctttg aaagagtttg
     7561 attataacgc acgggtggaa cttatcaatc ccattgcgga caccgttgct actgccacct
     7621 atcaaggagc agatgttgac tggtatatca aggcagacga tattgtgctg acaaaggatt
     7681 ctagttcatt caaagctcaa ccacaagcaa agaaagaacc gacacaagac aaatagtcgc
     7741 taggtagaaa ggagactttt tcgcatgaaa cagcgtggta aaaggattcg cccatctggt
     7801 aaagatttag tctttcattt tacgatagcg tcactcctgc ctgttttcct gctggttgtc
     7861 ggactgtttc atgtgaagac aatccagcag atcaactggc aggattttaa cctatcacaa
     7921 gcagataaga ttgacattcc ctatttaatt atcagtttca gtgtcgcaat tcttatctgc
     7981 ttgctggtag cgtttgtatt caaacgggtt cgctatgata cggttaaaca actttaccac
     8041 cgtcaaaaac tggcaaagat gatacttgaa aacaagtggt atgaatctga acaggtcaaa
     8101 acagagggtt tctttaaaga tagtgctggt cgtacaaagg aaaagataac ctacttccct
     8161 aaaatgtatt atcgacttaa aaatggcttg atacagatac gggtggaaat cacgctggga
     8221 aaatatcaag accaactctt acacttggaa aagaaattag agagtggctt gtactgtgag
     8281 ctgacggata aagagttaaa ggattcctat gtggaatata ctttgctcta tgacaccata
     8341 gccagtcgta tttctattga tgaagtagaa gctaaagatg gtaaacttcg cttaatgaaa
     8401 aacgtatggt gggaatatga taagctccct catatgttga ttgctggtgg tacaggtggc
     8461 ggtaaaactt actttatact gacactgatt gaagccttgc ttcatacaga ttcaaaactg
     8521 tatattcttg acccgaaaaa tgctgacctt gcggacttag gttctgtgat ggcaaatgtc
     8581 tactatagaa aagaagactt gctttcttgc attgaaacat tctatgaaga aatgatgaaa
     8641 cgtagtgagg aaatgaagca gatgaagaac tataagactg gcaaaaatta tgcttactta
     8701 ggtctcccgg cacacttctt aatctttgat gaatacgtcg ctttcatgga aatgctggga
     8761 acaaaagaaa acaccgcagt tatgaataag ctgaaacaga ttgtcatgtt aggtcgtcaa
     8821 gctggcttct ttctaatact ggcttgtcaa cgtccagacg caaaatattt aggcgacgga
     8881 atccgtgatc agtttaattt cagagtggct ttaggtcgta tgtctgaaat gggctatggc
     8941 atgatgtttg gcagtgacgt acaaaaggat ttcttcttaa agcgaatcaa aggtcgtggc
     9001 tatgttgatg taggaacaag tgtcatatca gagttttata ctccccttgt accaaaagga
     9061 tatgatttct tggaggaaat taaaaagtta tccaacagca gacagtccac gcaggcgacg
     9121 tgcgaagcgg aagtcgcagg tgtggactga tcttgctggc tggtgtggca atagccacgc
     9181 cagcacttaa ccccccgtat ctaacagggg ggtacaaatc gacaggaaac agtcaaaaaa
     9241 acattagaaa atcctttggt tacaagggat ttacaaaatt tcagcgtatg tcaaatgggc
     9301 tttaaaagtt gacatacgcc tttttgattg gagggatttt tactgaatga acaaacttgg
     9361 ttacagcatt taaaagaaaa acgcttggct tatggactat ctcaaaaccg tttagctgtt
     9421 gcgactggta ttacaaggca gtatctaagc gatattgaaa caggaaaagt caagccatca
     9481 gaggatttac agcagtccct ttgggaagct ctggaacgct tcaatcccga cgctcccctt
     9541 gaaatgctgt ttgattatgt aaggattcgc tttccgacaa cagacgtaca gcaggtggtc
     9601 gaaaacatct tacaactgaa actgtcctat tttcttcatg aggactatgg tttctattct
     9661 tattcagagc attatgcttt aggcgacata ttcgtccttt gctcccatga actggacaaa
     9721 ggagttctgg tggaattgaa aggtcgtggg tgcagacaat ttgaaagcta tcttctggca
     9781 caacaaagaa gctggtatga gttctttatg gacgttttgg tggctggcgg tgtgatgaaa
     9841 cgccttgacc ttgccattaa cgataagaca gggattttga atatccctgt actcactgaa
     9901 aagtgccaac aggaagaatg tatctccgtc ttccgcagtt ttaaaagcta tcgcagtggc
     9961 gaactggtac gcaaagagga aaaggaatgt atgggaaaca ccctctatat cggttcatta
    10021 caaagtgaag tttatttctg tatctatgaa aaggactacg agcagtacaa gaaaaatgat
    10081 attcccattg aagacgcaga agtaaaaaac cgttttgaga ttcgattgaa aaatgagcgt
    10141 gcctattatg cagtccgtga tttactcgtc tatgacaatc cagagcatac cgcctttaaa
    10201 attatcaatc ggtatatccg ttttgtagat aaagacgatt ccaaacctcg ttctgattgg
    10261 aaactgaatg aagaatgggc ttggtttatt gggaacaatc gtgaacgatt aaaactaacc
    10321 acaaaaccag agccttactc cttccaaagg acgctgaact ggctatctca tcaagttgcc
    10381 ccgaccttaa aggttgcgat taaacttgat gaaatcaacc agacgcaggt tgtaaaagac
    10441 attctcgacc atgcgaaact gacagaccga cacaagcaga ttttgaagca acagtcagta
    10501 aaagaacagg acgtgataac aacaaaaaaa taactcaaat acaaattcat tgaatataga
    10561 gaggagaaca tttttatgaa ttttggacaa aacct
//
LOCUS       DAGCXG010000090        10488 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGCXG010000090 DAGCXG010000000
VERSION     DAGCXG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.093"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(216..701)
                     /locus_tag="MB022_002195"
     CDS             complement(216..701)
                     /locus_tag="MB022_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBT4467175.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    791..1136
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1181..1822
                     /locus_tag="MB022_002196"
     CDS             1181..1822
                     /locus_tag="MB022_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4467176.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1841..2476
                     /locus_tag="MB022_002197"
     CDS             1841..2476
                     /locus_tag="MB022_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4467177.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2494..3309
                     /locus_tag="MB022_002198"
     CDS             2494..3309
                     /locus_tag="MB022_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4467178.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3628..5064
                     /locus_tag="MB022_002199"
     CDS             3628..5064
                     /locus_tag="MB022_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4467179.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5253..7700
                     /locus_tag="MB022_002200"
     CDS             5253..7700
                     /locus_tag="MB022_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4467180.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7930..8997
                     /gene="rlmN"
                     /locus_tag="MB022_002201"
     CDS             7930..8997
                     /gene="rlmN"
                     /locus_tag="MB022_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBT4467181.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9190..10293)
                     /locus_tag="MB022_002202"
     CDS             complement(9190..10293)
                     /locus_tag="MB022_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4467182.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3468 a         1793 c         2281 g         2946 t
ORIGIN      
        1 tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga atcaataaat
       61 atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca tactagttgt
      121 tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga
      181 cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata aactggcaga
      241 tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta
      301 ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact
      361 tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg
      421 cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt
      481 tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt
      541 tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg
      601 acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt
      661 tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt
      721 tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt
      781 aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc
      841 tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa
      901 gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga
      961 taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc
     1021 tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt
     1081 tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat
     1141 ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt
     1201 tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat
     1261 tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct
     1321 acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact
     1381 gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc
     1441 actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat
     1501 acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca
     1561 aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa
     1621 tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt
     1681 aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt
     1741 ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac aagttgaagg
     1801 aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt
     1861 gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg
     1921 gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt
     1981 ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc
     2041 attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt
     2101 tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac
     2161 aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt
     2221 attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag cgattgccag
     2281 agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc
     2341 tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca
     2401 aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt
     2461 ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct
     2521 ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat
     2581 tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct
     2641 atgggattta gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca
     2701 gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa
     2761 tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca
     2821 agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca
     2881 cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa
     2941 aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc
     3001 aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc
     3061 agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga
     3121 aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt
     3181 ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta
     3241 gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg
     3301 agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa
     3361 acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc acaacctctt
     3421 ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc aaaaatgtag
     3481 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc
     3541 ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat
     3601 tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa
     3661 gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat
     3721 gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga
     3781 acgaacggtg atttggcagt agaccactat catcgttata aagaagatat tgctttaatg
     3841 aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat
     3901 ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg
     3961 attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg
     4021 caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca
     4081 gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa
     4141 aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg
     4201 aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa
     4261 tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat
     4321 ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc
     4381 aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta
     4441 gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct
     4501 aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta
     4561 aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc agaagaaact
     4621 ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac
     4681 tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc
     4741 atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa
     4801 caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa
     4861 gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt
     4921 agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca
     4981 caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt
     5041 gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat
     5101 ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga
     5161 aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt
     5221 ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa
     5281 aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga
     5341 ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt
     5401 taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct
     5461 gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg
     5521 gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac
     5581 atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg
     5641 atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga
     5701 ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga agttatttag
     5761 cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc
     5821 tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg
     5881 ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga
     5941 aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga
     6001 agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa
     6061 atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc
     6121 ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt
     6181 tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac
     6241 aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg
     6301 atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc
     6361 aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat
     6421 tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata
     6481 tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac
     6541 tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg
     6601 aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg
     6661 gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg
     6721 gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc
     6781 cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg
     6841 gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac
     6901 aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa
     6961 atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc
     7021 caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca
     7081 gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc
     7141 atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa
     7201 atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg
     7261 attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg
     7321 agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg
     7381 ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg
     7441 aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag
     7501 cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg
     7561 gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt
     7621 cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc
     7681 cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa
     7741 aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg
     7801 gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga
     7861 agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg
     7921 gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg
     7981 tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa
     8041 aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta
     8101 tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt
     8161 acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga
     8221 caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg
     8281 ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca
     8341 cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat
     8401 gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg
     8461 catatcatca atgatgccaa aggattggca attggtgctc ggcacattac agtttccact
     8521 agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct
     8581 atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt
     8641 ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga
     8701 atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa
     8761 ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat
     8821 ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat
     8881 gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt
     8941 gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg
     9001 tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt
     9061 caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa
     9121 aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt
     9181 agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt
     9241 ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt
     9301 aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag
     9361 attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc
     9421 aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc
     9481 tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc
     9541 catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat
     9601 atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc
     9661 ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct
     9721 atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa
     9781 agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc
     9841 aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc
     9901 gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt
     9961 gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc
    10021 accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat
    10081 atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt
    10141 caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac
    10201 tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat
    10261 ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa
    10321 cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa
    10381 cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta
    10441 tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaat
//
LOCUS       DAGCXG010000091         9935 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGCXG010000091 DAGCXG010000000
VERSION     DAGCXG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.094"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            574..1728
                     /locus_tag="MB022_002203"
     CDS             574..1728
                     /locus_tag="MB022_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBT4467183.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1722..2144
                     /locus_tag="MB022_002204"
     CDS             1722..2144
                     /locus_tag="MB022_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467184.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2446..2826
                     /locus_tag="MB022_002205"
     CDS             2446..2826
                     /locus_tag="MB022_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBT4467185.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2808..3722
                     /locus_tag="MB022_002206"
     CDS             2808..3722
                     /locus_tag="MB022_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4467186.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3962..4651
                     /locus_tag="MB022_002207"
     CDS             3962..4651
                     /locus_tag="MB022_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467187.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4771..4857)
                     /locus_tag="MB022_002208"
     CDS             complement(4771..4857)
                     /locus_tag="MB022_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4467188.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            5503..6435
                     /locus_tag="MB022_002209"
     CDS             5503..6435
                     /locus_tag="MB022_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4467189.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            6452..6922
                     /locus_tag="MB022_002210"
     CDS             6452..6922
                     /locus_tag="MB022_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4467190.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7006..7310
                     /locus_tag="MB022_002211"
                     /pseudo
     CDS             7006..7310
                     /locus_tag="MB022_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7585..8766
                     /locus_tag="MB022_002212"
     CDS             7585..8766
                     /locus_tag="MB022_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4467191.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            8856..9125
                     /locus_tag="MB022_002213"
     CDS             8856..9125
                     /locus_tag="MB022_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBT4467192.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
BASE COUNT         3689 a         1579 c         1638 g         3029 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtta actaacgcta taaataacga ttatgttccc
      481 aaaacaatat tttatgaact ataacaaaaa tttgttatcg catattgtat attttaatta
      541 taggtgtata ccacttaaat ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa
      601 atttatctag gtctcctctt ttccttgatg attggaattt ttagtatagg aattacaaat
      661 acctatgctt cagaaaattc taatttcaat atcaactccc aaccaattga agaacaagca
      721 attaattttg cgaaaactga ttttgaacac tccataactt caattctttt aatggaaaat
      781 aacaccgaaa ctgatattaa ctatactctt ggttcgccat ttaaaatgct cgatggagaa
      841 aatacggcaa atagttctat tttctttcca gtattttcag acgctgataa tacgattaaa
      901 tatatttata ccgtcaaaaa aaatacaaat ggagaatatt caggaagtat ttcacaattt
      961 ttagcaaaag aacttcaaaa tctagtagat agtaatataa cagatacaac cttatttttt
     1021 caagataata gcatttatta ttccgataat ggacaaatta aactactttg gaaatcacct
     1081 ttaagtacag aaaaaaatgg tgtttcttcc tttacagaaa aaaatgatct tatatccgta
     1141 aatataaaaa ataaaattaa aaatttctct aaaaaacaaa attttcctgc gattaaacgt
     1201 gtaaaaagtg tctcctccgg agataacttt attatgatta attggcgtat agcagaaacc
     1261 caaacaagtt ttccgtggtg tgctgcttac gtagaagctg ctatcttatg taacaaaaca
     1321 gatgtacatg ctacttcagc tagagccatt atgcattata cctatcctaa tttaaacaac
     1381 agtcaaattg aatcaaaaag tcttaatcaa gatcaaataa taaaatatgc aaattactgt
     1441 ggtgtttatc ctaaaaaagt agcacgaata ctaaaccttt cagaggtcca aacacaacta
     1501 agaaaaggaa atgccattta tatgggaact gctggttatg gaaattatag taacagccgt
     1561 catgcacttg cattatttgg gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg
     1621 aatccttggt ataactaccc aactgtaata agtgcaaatg caggaccagt tacaattcct
     1681 gtacctggag gtggttttac ttgggacaat accattacaa attggtaaat aaacccattg
     1741 ctttagttat gggtctagtg ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa
     1801 tacaaaagac tgatctgatt ccttttctag gaacttggca aactaaagat cattcagaac
     1861 gtctagtaat cactaattta aatacttcaa aaaatataga acaatatacc atcttagtaa
     1921 aagagaacaa tcataaaatt caaatcattg ataaaaattt aaatataaat gaacaagttt
     1981 tcatttacaa agcaacaaat aatttactaa cactgcgctc taataaaaaa caattagatt
     2041 acacttttag tttggattct ccaaaaaagc tcgtatttat tttgacaacg aatgatggga
     2101 cagaagggac agctaaacca attgaatttt ttaaagctga ttgatgaata gttgtttgat
     2161 attacaaaac aataatttca ccgaaggtga aacaagcgaa actttacaaa aagtggctag
     2221 catttgctag ccttttttgt attgtggagc gtgggttttg agggggattc aagggggagc
     2281 ttgcgcccct ttgacaagcg gttatttgca ccaaatacgt aggtatttgg ctagcaaata
     2341 tagagcttgc caaacccttt tagtgagctt tactcattgt acctttttag gtacaatggt
     2401 attgcgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag cgaacaaaat
     2461 caaaatttgg ctagcgatca ttctaaaaaa tatacctatc gatccgagcc aaaacaaatc
     2521 agttttcgag tgagcgaatc tgaatttgca aagctgaagc agtcagctga agctttgcaa
     2581 atgagtgtgc cggctttcgt aaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa
     2641 gtggcgccag acattgccca ggcaatcgcg cgcgatttag ccaaagctgg agggaatatc
     2701 aatcaaattg ctaagtggtg taatacacac caacacgacg ttgctcctgg agacgcccaa
     2761 cgcttatccg aaaacctaaa aatcatgcaa aaggagctac aaaagatatg gcaacagtta
     2821 aagtaagccg aaccacttct tgtagtcgtg caattaatta cgctgaacca cgagcaacca
     2881 ttaaaacagg aataaactgt gacgtcgatt atgccaaaag tgaaatgaaa caaattcgca
     2941 tgctgtatgg aaaagacgac cgtgtacagg cacacttatt gattcaatct tttcgaccag
     3001 gagaaattac cgctgaaaaa gctaatcaac taggggcaga gtatgcaaaa aaaatagccc
     3061 ccgatcacca aatagctatt tacacacata cagacaaaga ccatattcat aatcacattg
     3121 tcattaactc cgttaatctg gagactggaa aaaaatttca agctcatggt caagcatttc
     3181 taaacaaatg ttatgaccta aatgacgaga tttgtcttgc acataactta ccaatcactg
     3241 agcgaggaaa gaaaccagaa aaacgaacca tgtctgaaat aaaattaaaa gaaaaaaatg
     3301 aacctgtttg gaaagacgaa attcggattg cgatcgatca aacaatgaaa aacaaaaaaa
     3361 ccagaaccta tgaccaattt tgtaattccc tcaaaacatt ggggattcat tgcttcaatc
     3421 gcgggaaaaa tttcacttac gaattgatcc acaaaaagaa aaaagtacga tccaacagac
     3481 taggaaagga ctacgaaaag gagacgattt tacatgagct ggacagacga gaacgaacct
     3541 ataaccaaca gcgaaccaac gagctacgaa gagctcgagc tagcattcgc gaaactgcag
     3601 aagaagtacg aagagaacaa cgaactctta cgcctgaaca accggaaatt agaccggctc
     3661 gaagaaacca tgaacaacac gttgaaagag gaacagacca ccaacaagaa ttatcgctct
     3721 aaaacgttaa aagacataga aaacgaaata aagaaacaac gtgcttttct cgttccttat
     3781 acgcaagaaa ggatacaaac tacgattgat ttacaagtta aaaaactcca tcaagagcta
     3841 ttagaccagc gaaataagga acagaaaaag gtgcttattc aacgatcatt ggattttctt
     3901 gcccccttat gttgcttagt cctgacactg ttcgcatttt ggttagtatg gggaggctaa
     3961 aatggcattt tatttaccct atctactcat ttttgttagt atttcaggaa gtatttggct
     4021 catctataaa atttttcaaa caagatattc attaaaaggc tctaaaattc gatttaagcg
     4081 atttttcttt cttggctgta tattttcctt gataatcgtt tcaagcggct tattcggcgt
     4141 tttagaggga aataagcgcg tttctagaag tattttatta ggaaatgtca ctcaaaagta
     4201 tgaatcagcg cgtgataaaa agaaaaaaga gcaagtatta gcacaaaaaa tagaaaaatt
     4261 taccgcttgt tatgaggaca tgaatgatat ttttgtgaag caagaaaagc gtttgacgga
     4321 taaaaatatg gaaacactca ctcgacttta tcggaatttg ccagaagaac agcaaaaaga
     4381 gtaccaagaa aaatatgaac aagtaaaaaa agacgtgcaa tatgtgaaag atacgaaagt
     4441 ggaagaagct tgtagtgatt tatttggaga tacaattcct ttttcaacta gcgaacaaga
     4501 acgaaaagaa cgacaacaat cagtcacata tgaacgctat gaaatcttgc ttcaacaagc
     4561 aattaacata caaaatctga ctaaaaaaga aacagcttta aattatttaa aaagcgtcaa
     4621 ggaatggtta gatcagcaac agcaaaattg aatttgattg aaattcatgt taaactaaac
     4681 atgcaaatgt tcaaacatta gatacacgaa agccccagag aggtcggatt ctctggggct
     4741 ttttttaggt tgatgtcgcc tttactgatt ttacttcttg tttcggttat tcaaccaata
     4801 actgaataca gagatacaaa ctcctacaag aagggggcaa ataaacagtt caaacatcag
     4861 atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat accctacaaa
     4921 attaaatctg tgtggcaatt ttaagtatct cttatttccg ggttgttagg gcttgccctg
     4981 accgcctgta gggcgcttta aatgccagat aaaggttttt atactaagac tgtcttaaaa
     5041 cggcttagac aagctcttag agcctcaacc ctcctttttg aacaatggtt ttagcaaagg
     5101 agctggaggc gactgcgcag gtataatgtg agagcgtagc gatcacacct ttttggcgac
     5161 cgtagggagc gaggttttga attagcagtt tagaaaagtt cgcgaacctg tttaaagaaa
     5221 aaataattgt ttttttaggt gggaaatttt gtctccctct tttataatct ttttttaaac
     5281 ctctttttaa acctctttac cgtcggctgt gtccttactc ccccaaggga tacaaaattt
     5341 tataggcatg aaattatctg tttataggca tgaaattatc tgtttatagg catgaaatta
     5401 tctgtttata ggcatgaaat tatctgtttt aggtattgtt tttagacata atatgtccta
     5461 aaatgatgtc aataaaatca tttagactgg agtaacaact aaatggcaaa tgaaatcgta
     5521 aagtatgaac cagaattaaa taccattcct ttaagaaaat ttaccccaat agaaatgaac
     5581 ttgttttttt ctattgtctc tagaatgcgc gataaaggta atcaaaaaat acgcttttat
     5641 ttcagccaac ttaaagattt aagtgcgtat aagcagactg caggagaagt ctttataaaa
     5701 gatttggcaa gaacatacga tcatttgatg gatttacgtt tcggaagaaa aagcaatggt
     5761 ggattaacaa tagaacgatt tacaatgttt actgattttg aaattcatgg agaagtcgat
     5821 aacccttatg tagatataaa agtccacgaa aaagctttgc ctttacttaa taatctagaa
     5881 agctgggtac gtttctcttt aacagaattt cgagatttaa atagcagtta tgctaaaaca
     5941 atgtttagat tgctaaaaca ataccgaact caaggatggg ctgaattttc taaggaagat
     6001 tttttagagc tattagatat tcctaaaagc tatagacaaa caaatataaa tcagtttgta
     6061 ctcaaaccaa taaaagaaga actaactcca ctatttaaag gattaactat tcgtaaaaaa
     6121 tatggtaaag gtcgaggtaa acctgttatt ggttatcgtt ttacttggaa agctgaaatc
     6181 aaccatgctg acgatttttc aaaagggaag caagaagatt tacgtataaa attatttaat
     6241 attgaacaca atggagaatt aacacaaaaa gaaaaatggc gtgctaaaga tcgaatacta
     6301 aatcttccgt taggtactca tgaagctgat tttaaaaaac aacaacaaac cgaaaaagaa
     6361 gaagcggaaa aacaatcaat ctcaaatgag cttaaacaag atttattaga aaacttacaa
     6421 aacttattcg attgaagcat aaggagtatt tatgatagat aaaacgatca aagaactcgc
     6481 ggaagaattt catgtttcaa aacaagcaat taggaaaagg ctatcagctg attttagaga
     6541 aaactatgtg caaactgtta ctaggaacgg agtgaaaacc ttagttgtca gctacaaagg
     6601 atatttgctt ttaaaacaac attttttagg tagtaacacc actggtaacc aagtagaaac
     6661 cgttgctagt aacgatgaaa actcgattat tgttttttta catgaacagc tccaagaaaa
     6721 agatactcaa ataaaacaaa tgcaaaagtt actcgaccag caacaacaac tcaccttgca
     6781 ggcaaatcaa cagatcgaaa aactgcaaga acagttacaa ttagcttata cggaagaacc
     6841 tgaagaagag gaattaactt ctgtcaataa agaaaacaac aatgcaataa caacagaaaa
     6901 aaagtggtgg aaattttggt aacaattacc aactttccat aaagaagaaa tcaatttcta
     6961 tagactctat ctttctaaga ataaaaaata tgtttaggtg atcaaatgac tataagtaaa
     7021 gtaatcaaac aaaaaagaat agaaaaacaa ctaactcaag aagatttagc tgaaatgctt
     7081 ttagtatcta aaaaaaccat ttctaattgg gaaaatggtc gaacaatacc agatacagaa
     7141 aatttaattc agtttgctaa gttatttgat ttatcactag acaatatttt gttaggagaa
     7201 gaaaaaatta tggtaaaaaa tcatacaaaa acactaatac tattatttca ttttgtaatt
     7261 tttttaagtt tagttttcac cttaattcag tccggattct tttattttag ccattcaatt
     7321 gccacttggt tattaatatt ttttctaagt ttactattaa taattaatac tttaattatt
     7381 atctttaata aacatagaaa cttatttctt cacagcatat ttccaattgt tttattaatt
     7441 tttctatact ttatataaaa agatagaact atttagtaag ataaaaaagt agctaattgt
     7501 aattttcgtc taaaactcaa aactagtaaa aataacttta ttgtgttatt tttatttata
     7561 aattatgaaa aaggaccttt cgaaatgaaa aataatagaa tctacaaaaa tcagttgaat
     7621 tatgggcttt tattgccagt ttttctgcta tgtatcattg gattactttc tttatatgtt
     7681 gctttatatc acgactctta cactaattca aaaatcacaa aagaattaat taaacaagta
     7741 ttgtggtata gtattgggtt cacttcagca ataactattt atttttttaa tcctaaaatt
     7801 ttgtggaaat taactcctat attttacgga ttatgcatca taatgttatt acttctcttg
     7861 aaattctata atccacaatt agcagtctta actggagcaa aaaattggtt ttatattgct
     7921 ggtttttcgt ttcaacctac tgagttagtc aaaataggat acacccttat gctagctaaa
     7981 ataaccgtca attatcagca aaaatctatt actagtaata cgaaagatga ttggatttat
     8041 ttacttaaaa cttttattgc tactcttcca ataggaatat tattattttt tcagaacgat
     8101 ctaggaacaa tgttagtctt cattgttatt tatattttta ttttgtttac gtctaacata
     8161 aatataaaaa taattgcccc aataatcgcc attggtgttg ttttatcctt aatattttta
     8221 tatttggtta tatcagatac aggaagagat ttcttattaa aaataggatt tcacgactat
     8281 cagtttaaaa gaatagactc atggttgaat ccatttttag atccaaatgg atctagctat
     8341 cagttagcaa atagtttaat tgctattggc tctggcggac ttttaggtac tggttttaat
     8401 gtatcaaaca ttcacgttcc tgttagagaa tccgatatga tattttctgt aattggagaa
     8461 aattttggct ttataggaag ttgtcttgta atattcctat acttttattt aatttatcaa
     8521 atgtataaaa catgttttaa aagcaataac ttatttttca cctatatcgg tataggcatt
     8581 gttgcaatga ttttttttca tgtttttgaa aatataggtg caagtatcgg attactacct
     8641 ctaactggta ttccactccc ctatattagt caaggtggtt ctgccattct tagtaatttt
     8701 ttggggttgg gatttatgtt gtgttgtagt agatatacta acgaaaaaaa aggaacacaa
     8761 aaatgaattc aaaataacac tttatgaata gaatgtctat ttcaataaaa attatgatac
     8821 cttaaagtta atcattaaaa aaggagtgtc ataatttgaa aaaaacaaaa ttattggtag
     8881 ctagtttgtg tttgtttagc agtttattag ctttcacccc ttctgtttca ttttctcaaa
     8941 atggtggagt ggttgaagct gctgctcaaa gaggatatat ctacaaaaaa tatccaaaag
     9001 gtgctaaagt gccaaataaa gttaaaatgt tagtaaatat tagaggtaaa caaactatgc
     9061 gtacttgtta ccttatgagt tggaatgcta gtagtagaac agccaaatat tattactaca
     9121 tttagtaaaa acaaaacatt ctttctatac aataaaatag cgtatagaga ggatgttttt
     9181 aatttgaaaa ggagtggtta tctttatgaa aaatagatca tttgctatat tagtattatc
     9241 cgtaatttta ttagatatat tgttaataac attagcttta aaaactttaa atttagtaac
     9301 aagttttgtc attataccag taattatagg aatgtctatt tatttcatca aaaaaaataa
     9361 acaatttttt tgaatgaaat tacgggcgaa aataatcact caaaattatt aataaaaacg
     9421 aatcaactac tttttctgaa gaaacataca accgtaaaca acaattgttg tttacggttg
     9481 taaattatca aactatatgg tgacttgttg cttaaaatag gttgaaattt cgtttatttt
     9541 ttttgagaaa ttttacccac gtactaccca tgtacctatc cacgtattac ccatgtaccc
     9601 ccccatgaca aatgggcgga ggggcttgcg gcgcttggta ttaaaggcat tatccgcctc
     9661 ccaggtttct gaccattttc tctcataagt ggggttttta ccgcgttgag agaaaatggt
     9721 cagaaaccta aacgatcggc aaagaaaaca taccaagcgc cacaagcaaa aaaagttaga
     9781 gtaacttcca gaaaagaaaa atttttagaa caacttttga aaatttgatt ttgatttata
     9841 tccgttcaat ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg
     9901 gtagcgttaa aaaaagtgga ggtataacct atgtt
//
LOCUS       DAGCXG010000092         9861 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGCXG010000092 DAGCXG010000000
VERSION     DAGCXG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.095"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            205..684
                     /locus_tag="MB022_002214"
     CDS             205..684
                     /locus_tag="MB022_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467193.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="MB022_002215"
     CDS             717..2945
                     /locus_tag="MB022_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4467194.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="MB022_002216"
     CDS             3074..4717
                     /locus_tag="MB022_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBT4467195.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="MB022_002217"
     CDS             complement(4790..5335)
                     /locus_tag="MB022_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBT4467196.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="MB022_002218"
     CDS             complement(5358..5843)
                     /locus_tag="MB022_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4467197.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="MB022_002219"
     CDS             complement(5833..6645)
                     /locus_tag="MB022_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBT4467198.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="MB022_002220"
     CDS             complement(6784..8316)
                     /locus_tag="MB022_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBT4467199.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="MB022_002221"
     CDS             complement(8425..9456)
                     /locus_tag="MB022_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4467200.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3279 a         1832 c         1899 g         2851 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcag cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg a
//
LOCUS       DAGCXG010000093         9694 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGCXG010000093 DAGCXG010000000
VERSION     DAGCXG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.096"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            21..233
                     /locus_tag="MB022_002222"
     CDS             21..233
                     /locus_tag="MB022_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467201.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(266..460)
                     /locus_tag="MB022_002223"
     CDS             complement(266..460)
                     /locus_tag="MB022_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467202.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(468..656)
                     /locus_tag="MB022_002224"
     CDS             complement(468..656)
                     /locus_tag="MB022_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467203.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(653..793)
                     /locus_tag="MB022_002225"
     CDS             complement(653..793)
                     /locus_tag="MB022_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467204.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(1283..1648)
                     /locus_tag="MB022_002226"
     CDS             complement(1283..1648)
                     /locus_tag="MB022_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467205.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(1688..1990)
                     /locus_tag="MB022_002227"
     CDS             complement(1688..1990)
                     /locus_tag="MB022_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBT4467206.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(1990..2289)
                     /locus_tag="MB022_002228"
     CDS             complement(1990..2289)
                     /locus_tag="MB022_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467207.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(2282..2446)
                     /locus_tag="MB022_002229"
     CDS             complement(2282..2446)
                     /locus_tag="MB022_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467208.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(2452..2721)
                     /locus_tag="MB022_002230"
     CDS             complement(2452..2721)
                     /locus_tag="MB022_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467209.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKQLNELEQV"
     gene            complement(2733..3482)
                     /locus_tag="MB022_002231"
     CDS             complement(2733..3482)
                     /locus_tag="MB022_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467210.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3485..4171)
                     /locus_tag="MB022_002232"
     CDS             complement(3485..4171)
                     /locus_tag="MB022_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467211.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(4177..4848)
                     /locus_tag="MB022_002233"
     CDS             complement(4177..4848)
                     /locus_tag="MB022_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBT4467212.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4841..5182)
                     /locus_tag="MB022_002234"
     CDS             complement(4841..5182)
                     /locus_tag="MB022_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467213.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5360..6058)
                     /locus_tag="MB022_002235"
     CDS             complement(5360..6058)
                     /locus_tag="MB022_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBT4467214.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6145..6615
                     /locus_tag="MB022_002236"
     CDS             6145..6615
                     /locus_tag="MB022_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467215.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6658..6837)
                     /locus_tag="MB022_002237"
     CDS             complement(6658..6837)
                     /locus_tag="MB022_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467216.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6824..7162)
                     /locus_tag="MB022_002238"
     CDS             complement(6824..7162)
                     /locus_tag="MB022_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBT4467217.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7178..7381)
                     /locus_tag="MB022_002239"
     CDS             complement(7178..7381)
                     /locus_tag="MB022_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467218.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7396..8172)
                     /locus_tag="MB022_002240"
     CDS             complement(7396..8172)
                     /locus_tag="MB022_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBT4467219.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8471..8791
                     /locus_tag="MB022_002241"
     CDS             8471..8791
                     /locus_tag="MB022_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4467220.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8809..9240
                     /locus_tag="MB022_002242"
     CDS             8809..9240
                     /locus_tag="MB022_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBT4467221.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            9370..>9694
                     /locus_tag="MB022_002243"
     CDS             9370..>9694
                     /locus_tag="MB022_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HBT4467222.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQ"
BASE COUNT         2788 a         1897 c         1502 g         3507 t
ORIGIN      
        1 atttaaaaag gtgatttatc atgaatatca acaatttagt aaaactcgtt gaacaacgtt
       61 ttaaagaatc aggattagaa caattaaaaa aagataattc tccagaatta ttttctaatt
      121 ttaccgaaga cgacattcct actcttaaac aattaaaata ttattctgat tattggacgc
      181 aaactcgtga gaatgaatta aatttagaaa aactcattat taaaaagaaa tagcaactcc
      241 agcccacacg gctggttttt tagtttcaac ttccctcctt acgggtcaaa atcatcggtt
      301 ccggcataat ctgatcatcc tttagcaaca aatcaggata ttttttcctt agcttcttat
      361 tgttcgtgta agttttcaat agccattggt tggcttcgct tttgctttct ccagaaaaat
      421 aaattttccc tgtttggatg cctgcaagtt gaatcatcat tttcacctca aatcaaattt
      481 ttttctgcta gatggagcaa aagcaatccg acatcaagaa tcttttgacc cattatttca
      541 gctactttgc tcggcttaac tccttgagaa aagagcaagc gtgcttgctc tatctcttcg
      601 tgagtccaaa taaatttggc ttcctcaagg ataattactg gatcagttgc tctcattgtt
      661 cctctggaaa aatgatgtca ttcttatgct tggaccaggt atctgcatat ggaacaaagt
      721 attgtctagc taattcgatt tgatcaatta aagcagtttc tgataattca tggtctgcag
      781 caatttcatt catactatcg ccttcatcta aacagataag accgttgacg ttatcgattt
      841 ttacatttgt gactgcttga ctttctccac gctcataata ttctacagtt gatactgata
      901 catcagcttc ggagtttaat tgcagaatct gttactgccg ggataattta atattctttt
      961 cataattcac cttttttcct actgacatcg aatatttttc atgttatgat tagccgtgaa
     1021 aggagagcta tattatgtgt ttacacatga tgtccttagc tcattcggtt ctttggaagc
     1081 ttttttggaa ataactctca ggttcttgac gcaaatctca tttattcttt gaaacaaaaa
     1141 acttccattt tccgagagct ttggatcact tcaaaagaga tttgcattta tatttctgtg
     1201 accttgagtc gcgtaaagaa ccttacagca atggcggcaa ttccactttc aggaaaccgc
     1261 cttttttcat tgatattggt ggttagcgga ctacccattt tctttcggca gtgccaaacc
     1321 acaaatgatc tgtgttgatg tttaggtagt tgtataccag ataagctaaa tccaaagtgc
     1381 ctgcatcttc aagacgacaa tattccaaaa attctcccat agctggacat aaaaatcctg
     1441 gagctatcat atcccaacag ttaatagtgc aaagaaattc gtttagcttc tgaacgtttt
     1501 ctttggtttc acgaattaca acatactcca attcttctcc gctggcagag cattccaaaa
     1561 cttcaaattg attaagaaat ggtagaacat atgcttcaaa catcaacatt ccatctttct
     1621 gtttttgcac ttgttttgca tcaatcatgt ctatcctcct attcagttat ttcttccatt
     1681 aatggaatta tttcaggtca ctggacttaa cgaaaacacc attgaccatc tttccagtcc
     1741 gacctttgat ttcgtcataa gcaaaatcca agcactcgta taaatccata tcattttgca
     1801 ttgccaagat gatcaaggtt actactacat cgccaattcc gtctcttaaa ccatgttcat
     1861 cttttctagc tagagaagcg gcaacttccc cgatttcttc gaccgttttt aacatttgct
     1921 tgctgaaatc cgcttgatcc aatcccttat cttttgccca ctgctctact ttcgcgatta
     1981 gttcgtccat tatttctcct ccacatacct aaactgtctt ccttttgaat caatccataa
     2041 gctcctagct ctatcccaaa taatattttt gctcagacca gtaatttcag ataactgttt
     2101 agcggtaccc gttactagaa ttcgatcacc atgccagatt gcaatctttc tcggcgttct
     2161 ccgtttgggc ttttcagtcc acattgattt accgagcttt tggacttctg caactatttt
     2221 tttatcttcc tgccaatctt ctgaataagt caattcgata attcgctgca ttgccgcttt
     2281 cttatccatc ccgacattct cctttcagta atttgagtac ttgatcaagt gcgctctcac
     2341 gtccgccatg aaatgtgttg agccacttgt cttcgtacga cacactttgt cttaaagctt
     2401 cttgatgcat taattcgatc tgtgctgtaa atgtctttag gtccatcttg tttacacctg
     2461 ctcaagttca ttaagttgtt tttgcaagcc tttaacacaa tcaatgaata gtaatttggt
     2521 atatgctaaa tttcttaatt gcgttccatc gatatagagt gcgaaatagt atctgagttt
     2581 actccaactc gaacgatcat tcttaattcc ttcaattcca gcttcttcga gttgatcata
     2641 tacgtctctc aggacgtcta attcctcgcc ctttttataa ttcgctattt cattaattag
     2701 ttctagataa tcgatcttca tttttccacc tcttagaatg gtgcttttga ttgtctatta
     2761 gctcgttcta gcgctttttt cttgagatag gcttcttggt cgattgccca ttcaggaagc
     2821 ttctctcgtc ttcctgtgcg cttgtatcca ctgcttgcgt tcttaggttc acttttttct
     2881 ttccttgccc aacttcgaat agttgccaaa tagtttttat aagtcttacc agatgattca
     2941 caatactctg acagtcgttc gattcgctct tggtaatcat tagggaattc tgttttgagt
     3001 ttctccatct gctcatctga caaaagaaca tttttatact ctccgtattt atgacggacg
     3061 ggcttagcct tcgatttttt cgaaggcggt aactctctta tatattcttt tgtattatta
     3121 aatgtattat taatagatgt attattatct ttgacttttt cgtcaatagg ggtattgcgt
     3181 ttttcgtcaa tagggtattg attaattcgt aggtacctat tgattatttg attggtaccc
     3241 tctttgtaaa tgatttcccg attcaagtat ccaaacttaa tcaaatcact tacccatcgc
     3301 gatatggtct ctttattcac accatataaa tctgcaaagt actcattgcc tgcccaacaa
     3361 aagcctcttt cattacacaa ggccgttatc tctccgtata acaacttagt atttggttta
     3421 agtcttttgt cgtaccttac gttggctggt ataatcgcat aataacttcg atgttctgtc
     3481 atttttaccc tccaatattt aactttttga ttgttttctg gtttaatttg atccctttga
     3541 tttgatattt atttttgaaa ttaatcacac ctattttgtg cttctctgtg tgatggattc
     3601 tgcagagtgc tgcaaatgtg tactctgcat gatcaacttc tttgcgcttt cgtcttccta
     3661 gcgctttgtc aaagtgatcg atgtcagctc ctgttttgcc acagatgcag cagactcttt
     3721 ttgtgatgca tttgtagaag taatattctt gattcgcagg taaaatctca tagccttctt
     3781 tgaaaggaat atgatgttca aagatgaaat ctaagatgat atttgctaag acattggcat
     3841 cactcacagt tgtattcgat tcgtctttca ggcttatttt gcgccctgtg acaccttcaa
     3901 aacgaaagta gaagaattcc ttccagaagt ccgttggcat gcctgtatcg ataaaaatat
     3961 cgcctatcag cgcatagatg aagtttcgtt gctgtacggt gaaacgtcta ggatcaataa
     4021 aacgaatttc aatgactcga tcaccatcgt agccgtcata catcgtcttt agtcgatcaa
     4081 tgttcacttc ctcattgatg gttgcgctta tgtcttttcc tttgaacttt ttcagaaccg
     4141 ctgaatatga atcgattaat ggtttaaaca ctcatatcac ttcttatcta attcttttct
     4201 cttagctgct attgctcgct ccatcaaggc acattgctca tagcttaact gttcaatagt
     4261 ttcaacgtta tcagctaaga gccctaattt atctgtctgc tcattaacat attcgattaa
     4321 ggttttggtc atatctttac ccatctgctc attgaaagct tctagaatcg tctctagcat
     4381 gtttaatttc tttgtatcga ttctaggtgg tgttggaata tcttcccctt gaaatacata
     4441 taatcccagt ccgtgtagag ccaatgcttt cacaaagcat cgcttcaatg agttattgat
     4501 ttgcattgca tttggtttaa caactggttg gtttcgataa tctaaaacag gaaataattc
     4561 ggtttccgtg tgtcctttaa ccgttactga gacagataca taagtcccag tttcatccat
     4621 aagaaaaggt ttatattcct caacaagaaa gtcttgatga gttccagaaa caaccctgta
     4681 gtgtttgtac tcattaatag ttaccgttgc ctgtggatca ttctttttca taatctccca
     4741 cgcgtgagcc caagataaat aatcaaaatt tccttttttc ttgagaattt tatttaactt
     4801 acgactaaaa agtttttcaa agttcgttgt ccctttgatt tcactcatca aattctgcct
     4861 ccatttcagc aatgtatttc ttacctggtc cgtaataaga gatatcaatc aagttatctc
     4921 tgtcgtactc ttctagcgca tcaatcaagc catcttcgat gacgtaaata tattcaggtt
     4981 tattcgaatg cttcgataga tggataagat aaacatgatc ccaaatagtt acatggttgc
     5041 ctaaatcatc ttgatcccaa gctagttctt cattcgtcaa aagatttcgt ctgatttttc
     5101 gaccacttgt ttcctcaatt ttcggcttgc cccaatcagg atcaatcaaa tattgatcta
     5161 gagtggaaag ttctttttcc atatgctaaa atctccttat gatgtgtttt ctttgtgact
     5221 ctttgcttgc cggcggagtc acttttttat ttgttgccat gctttttgct tgtcaatatg
     5281 ttgttggctt aggatgattg gtttatagta tttccaccag caattagcaa ttgccgtccc
     5341 tattcttagc gcttcagctc tattcattgt catcaccaaa aagtctctgt tgtctgttca
     5401 gttgatcaat ttccatacgg atcgcagttt ctggcaacca catttcaata aatgaaacag
     5461 catcatcgaa tctcttacga ggtaactcgc catatcttgg gattgaaaag gtacgtttaa
     5521 attcagacca aaattttgag aatacttttt tgctgatttc ttcataagct cggctttctt
     5581 ttccccctag aactcccata actttcatat ttcctttttg cttaatttca aactcttgtt
     5641 gtccgctaat tcgcatagta tctttaagca tggaaacatc ttttttaaca tctttcattt
     5701 cttctaattg atagatcatc atatcttcaa ttgtttgagg aacagtattc ttgcgaataa
     5761 catcttccat ttcgttgaat gcttcaatgt atttttgttt gaagtagata gctttctttc
     5821 ctgtaaaccc catagccagc aagaaaaatc catctctact aatgaagaaa actcgtcgat
     5881 ttctgccata tgaatctggt tcattacctt ctacaaacat ctgtccaaaa ttggacacat
     5941 ctttttttag tgcatcaata tctcttaaaa catgttgatg ttttttctcg aagctttctg
     6001 ccacttgtaa gctcgtagtc acagcttctt tatttttcaa aattactaat tcttgcatta
     6061 tttcttctct cctttttggt ataatttagt taaaaactgg tggtgtttat tttgaatttt
     6121 tttcacttaa caatttttca atggatgtca ataataagtt ttatattgag tacgttttta
     6181 acagggataa aaataaaagg aatgcgccct caactagaag ttgaacttaa tgcttcttac
     6241 ttcgctgcag atatgattta tacaaaagta atcatttcta actattcaac agaaccagct
     6301 atgttagtta atttagaact ttcatccgct aacttaaacc gcagatggtc agctactcca
     6361 tttaagaaat taattgctaa aggaggatca gtcgatgata atagaatata ctctgaagct
     6421 gtaccgctaa atattcctcc taaaagtgct atatcttgtt accttgcatt tgaagtaggg
     6481 aaaattaatt ttaataaact ccttaatcac caaacaaaaa tgatatttac tctaaataga
     6541 acccaaatcc acaaaattgt tgatatcaaa aatacaaact tcccaataga gaaattagtg
     6601 aaagaattaa actgagtaat agtaatatac attctagttt ctcttttcta ttcattttta
     6661 gtcagccccc tcggttggct ttttcgctct gtactcagct tcagcttcat caagccccat
     6721 aaaaatccaa accatgtaaa caatcgttcc tatcaacgct tgcctgcttc cccaaagtcc
     6781 taaagcgtag atgattagcg gtgcgctgaa cactagcgct ctattaaatt ttcccatccg
     6841 cttgcctcct taagatttcc gaaaaatttg tttctaaaaa ttcaagtgtt ttacttctta
     6901 aaaatagata tgtgtctctt ccttcaactg gataatagac aaatccattt ttgtttttct
     6961 cgatatcgat aatatttcta tatcttgggt tctttaaaac tctagaagta aaccaatcat
     7021 attttctgtt aatccgttct agcacttctg gcaacgtcat ccatctacca gtatcatcag
     7081 ctttttttaa ctcctcataa tccacttggg agataattac atagccttct ggaattggga
     7141 tttttgcttc tagatattgc attagctgtt cctcccttta caattcgtac atagtgataa
     7201 tcgaatctat aattctgttt gcttctgcag aagtcttttt accgtttaaa attaaagata
     7261 agtagctttt gctaattcca aatctttcag caagcatggt gtaagttaag aactttgaac
     7321 tttcgacata ttctttgatt ttttctctat ctcgttgagt gatttctgca atgtcagtca
     7381 tactaaaact cctttctaac caatttcctc taaatccatt tgagggtaat atccttcttt
     7441 ttttagtaat tcgtagataa atagacgccc tttctgtgtc catttggtat tcattacaat
     7501 tttagtgcca ccatcggctt tcgggatctc agttgtatga gattttgtgt atccttggtt
     7561 catgtgtttt ttgcacaata accattggtt accgactttt ttctgaatac ctagtttatg
     7621 aagtaattta ttcatctgtt gtggagacat cccataatct gctgcaatct gactaattgt
     7681 tactgaatct gtagaagata atatgctatc taaataggag attttaggtt cgtactctgc
     7741 aatcttttgt tctgcgatta atcttccagt tctttcttct tttagttgag ttgctaattg
     7801 aatgattgta tctggattaa gcaaagcttc ttctactttt tctggagtta gataacctcc
     7861 atgttttcta attgctggca acacttcact tgttacccaa cgtttaaatt ttttggcaga
     7921 gggaagtttt gattttaaga ttaaactgta caaaccagat tcgttgatga ttgtcatctc
     7981 tcttgattga cctgaggtcg tgattcgcga ccccatctta tcttccaaat ctacatgccg
     8041 cgacaaagca tctttagtgt ttgaataacc caaaacactc gcaacgtctt tgcctacaaa
     8101 atatggttca tcatttacta aaattgtccg aacttcgttt tgttcgaaat taaaaatttg
     8161 cggtgtgttc atatttctca ttcctttctt ttatattcgt aaacaaattt aacaactttt
     8221 ttctaaattc aattgacagt atctgagtaa tactctatac taagagcata attaaataag
     8281 acataaaaac attgatttta aagctttctt ggcggtctgc atttacatat caatagtgtt
     8341 ttttgttgtc tttttagttg ttaaatctgt ttacaagata tagtatagct cattactcag
     8401 aattagtcaa attatttctg cgtatttttc taaacttttt ttgtaaacat ttagaaaggt
     8461 tgttttatcg atgaatacct atgaaataat aaaagacttg gcgaagagga aaaagatatc
     8521 tatacggcag ttagaaatta attttggata ttctaacggt tatctagcaa agtggaaaaa
     8581 taatactcct aattcagatg aattaagtcg gattgctgac tatttcgatg tctctgtaga
     8641 ctaccttcta ggtagagaag aaagagaaac ccctaaacat gtggatttat cagaagacga
     8701 tactgttttt tcttttgatg gaaaagaaat atctaaggag acaatgcgta aagcgattgc
     8761 aattgctaaa gctttagagg aaaatgaata gttggagtga tggtttgtat gtatttgaaa
     8821 ttgaaagaaa tgttgagcga atacaatttg aaagtagttt atatggagat gaaagagcct
     8881 ggtttctact atccaaaacc cagaatcatt tttttgaatg aaaatctata cggtgaaact
     8941 gcagaagcct ttcatttatc tcatgaactt gcacatttca gcgcctctca ttttgaattt
     9001 tcagttttgt acgatacttc tacaactttt cactcaaaat ttgaaacaga agcagataaa
     9061 atagctattc taattttatt aaatatctat attgagaacg aactgactga tgaatctcag
     9121 tttaatctcg aaaaatttat ggagtattat tctattcaga ataagctcag atacacttgt
     9181 tatgctgtct gccaatgcta ttttaagaaa aaatattctt acgcaaggca atatgtatga
     9241 atacatcaag aattattaaa aaaaccgatt tcagcaaaac attaaaaaag cccgtgctgc
     9301 aacacgggct ctttcctcat atatgagctt ctacaaaaaa aatcatatca cagaaatgag
     9361 gaagaaaaaa tggaaaaaga gattttaata aaatctccag gaaaaacctt gattaaagta
     9421 actgatgatt ctatttcaat tattagaaaa ggatttataa atttagttaa tcaaggtatt
     9481 aaaggagaaa aaactatccc ctttaaaaat atatcagccg tacaattaaa aaaaccaggt
     9541 atgagtaatg gttatataca atttaccttg ctaggcggaa acgaaagtcg cggaggtata
     9601 ttagcggcca cgaaagatga aaacaccgtt atgtttacaa aaaaatattg gaatgaaatg
     9661 gaaaatttaa aaaaatatat agagaagcaa caat
//
LOCUS       DAGCXG010000094         9295 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGCXG010000094 DAGCXG010000000
VERSION     DAGCXG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.097"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(26..694)
                     /locus_tag="MB022_002244"
     CDS             complement(26..694)
                     /locus_tag="MB022_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4467223.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            828..1115
                     /locus_tag="MB022_002245"
     CDS             828..1115
                     /locus_tag="MB022_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467224.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1531..3069
                     /locus_tag="MB022_002246"
     CDS             1531..3069
                     /locus_tag="MB022_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBT4467225.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3066..4031
                     /locus_tag="MB022_002247"
     CDS             3066..4031
                     /locus_tag="MB022_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467226.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4018..5340
                     /locus_tag="MB022_002248"
     CDS             4018..5340
                     /locus_tag="MB022_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4467227.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5403..5507
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5581..6324
                     /locus_tag="MB022_002249"
     CDS             5581..6324
                     /locus_tag="MB022_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467228.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6339..7331
                     /locus_tag="MB022_002250"
     CDS             6339..7331
                     /locus_tag="MB022_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4467229.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7343..8080
                     /locus_tag="MB022_002251"
     CDS             7343..8080
                     /locus_tag="MB022_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467230.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8070..8792
                     /locus_tag="MB022_002252"
     CDS             8070..8792
                     /locus_tag="MB022_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4467231.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8876..>9295)
                     /locus_tag="MB022_002253"
     CDS             complement(8876..>9295)
                     /locus_tag="MB022_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBT4467232.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3045 a         1603 c         1965 g         2682 t
ORIGIN      
        1 taaataaagt agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt
       61 caaatgtttc tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat
      121 acttcacttt attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat
      181 gtgtgagatc caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac
      241 ttttgccaat cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc
      301 ctgtgcttcc gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca
      361 tcaaactatt gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat
      421 gtaaaaagaa aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa
      481 aaagaaacaa tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag
      541 cgaatccaaa aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct
      601 tagatggaat attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc
      661 cgcacaattc tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt
      721 ttcagcaact tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa
      781 attatttttg gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag
      841 acttattgac aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg
      901 ctcaagaaca aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta
      961 tctttttatt tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga
     1021 tgaagaaccg tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg
     1081 gagtgaaaga gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt
     1141 atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa
     1201 tattcgttga acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg
     1261 tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac
     1321 aatttcgtat agatttttat ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc
     1381 gactttgttg cttggattct ttttttgtca gcatatttag aggtattttt ttcttttttt
     1441 tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta
     1501 tacttaaaga ataacagaaa ggaaggtcgc atgaataatt ttatacaaac attaagtgaa
     1561 agaaaaggcg agttgctgga agctgcattt caacatctgt ccatttctct catttctttg
     1621 ctgattgcag cactgattgc tattccttta gcgatttggg cagccaatca taaaaaaatg
     1681 gcagaagtct tattgcagat cacgggtgtg ctccaaacga ttccttcact tgccttatta
     1741 ggtttactta tcccatttgt agggatcgga actgttccag cattgattgc acttgttatt
     1801 tacgctttgc ttccgatttt ccaaaacacg tatattggtc tagctgaaat tgacccgtct
     1861 attgaagaag cagcggtagc atttggtatg tccagaatga gacgcttgct gaaagtagag
     1921 cttcccatcg cattacctgt cattatctca ggtatccgaa ctgccttagt tttaatcatc
     1981 ggtacggcta ctttggcagc attgatcgga gcaggtggat tagggacatt tattttgtta
     2041 ggtattgacc gaaatacacc tgttttgaca ttgattggtg cgatcagttc tgccttgctt
     2101 gcaattatat tcagcggact gattcgctgg ttgcagcata agaaacctcg atattccatc
     2161 attactttag cagcagtggt gtgtttattt ggcggcgttt ctttatatca gtctgccttg
     2221 tttaaaaatg aaaccatcac gattgctgga aaattaggag cggaacctga aatcctgatc
     2281 caaatgtata aagaattgat tgaagacaac acaaaaacag aagttgaatt aaaaccgaat
     2341 tttggcaaga cgagtttttt gttcaatgca ctggatcaag gtcaaatcga tatctatcct
     2401 gagttcacag gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct
     2461 gatttgacaa aagaaaaaac atatgacgaa gcagaagaat tattgaataa gcaattcgat
     2521 atgacattat taaaaccaat ggcttaccaa aatacgtatg ccttggcagt caagagatct
     2581 tttgcgaacg aacatcaact taaaaagatt tccgatttgg cagctatctc tgatcagatc
     2641 aaagcgggat tcacactgga atttattgat cgttcagatg gatataaagg aatccaagaa
     2701 gcatatggct tgaatttttc aagtgtacaa agcatggagc caagtttgcg ttaccaagca
     2761 atcaataatg gagatgtcaa tattgtggat gcctattcca cagatagcca gctcaaggag
     2821 tacgatctag tgactctaga agatgatcga gggttattcc cagcttatca aggcgcaccg
     2881 ctgatgaaaa aagattttgc taaagaacat cctgaaatcg ttaaagcact gaataaatta
     2941 agcggaaaaa taacagagga tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag
     3001 aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa
     3061 gatcagtgac taaattaatc gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg
     3121 tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt
     3181 ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg attatctaaa ccatcagcag
     3241 gagacatcta cttcaaagga aaatcgttaa atgaatataa tctgcagaaa atgcggtgga
     3301 atatgggata tgtgctgcag caaatcgctt tgttcccgac catgacagtc aagcaaaata
     3361 ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa aagagcagac cgtgtagatg
     3421 aacttcttca aaaagttggc ttatctcctg atatttatcg agatcgtatg cctagagagt
     3481 tgtctggagg agaacagcaa cggatcggga tcatccgagc tattgctgca agcccagatg
     3541 tgattttgat ggacgagcca tttagcgcat tagatccgat ttcaagaaac tcattacaag
     3601 aattggtttt gtccttacac gaagaattgg gcacgaccat cgttttcgtt acgcacaata
     3661 tggaagaagc aatcaagcta ggtgatcgta ttgcatttat gaaggatgga gaaattatcc
     3721 aatgtgacac accagagcag ttattgatga atcctaaaaa cgactacgtt cggcatttct
     3781 ttgatgaacc gaaacagacg aaagagtggc gagtagaaga tttagtcgtc aatggatatt
     3841 tcttaaatga aataccagaa aatgctcgtc aacaagtttg ctttgatacg cctatgaaag
     3901 aagtatattc gcttctttcg gtgtatccaa gcattgtgat agtagaaaag caaagaagta
     3961 ttgggtcgct gacaagtaaa gaaatctttg cttttttaag tcgagaggag gaaggaaatg
     4021 aaaacatatg atgcaatcat catcggttca ggtgtgagcg gcttaagtgc tgcttatgga
     4081 ttgaaagaag caggtaaaac agtactagtg gtcgaagaag atctatgggg cgggacttgt
     4141 cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg
     4201 gtaaaacaat tgagtggaaa aggttttaac gagatcccaa ctgccaactg ggaagaactt
     4261 caaaaattca aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa acaattagca
     4321 gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt tggatgattc atctattgaa
     4381 gtcaacgaag aagtcttcca tgcggattac ttagttttgg caaccgggca aagacctacg
     4441 atcttaccag tggagggaaa ggaatacttg aaaactagtg cagacttttt atcattgcct
     4501 gtactgccta aagaaatcat ttttatcggt ggtggttata ttgcttttga gctagctacg
     4561 attgccaatg ctgcagggag caaagtaacc attgtccatc ataatcaacg gccattaaaa
     4621 gaatttgagg cgtctttggt agaggaagcc gtccatcaaa tggaagcatc tggcattcag
     4681 tttgcttttg gtgttgaaac ccaaaagatc atttcagaag gtactcgcta tcgtttagtc
     4741 ggaaaagaga cggaattagt agcagatatg attttttgtg cgactgggag acagccgaat
     4801 acagaatctt tagcacttga gcaagcgaat atcgtctttg ataaacacgg aattgcagtc
     4861 aatgattatc tgcaaacgag caatccaaaa atttttgcgt gtggagatat tgtttcaagg
     4921 aaaaccccaa aattgacacc agtcgccact tttgaaggaa attatgtagc taagcggata
     4981 acggatgcaa caagcgaacc tatcaaatat ccgattatac ctacgatcgt ctacgccagt
     5041 cctaaacttg ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga tcaagtggta
     5101 gagatggatc ttaccagttg gttcacctat catcgtgtca atgaaccagt tgctaaggct
     5161 gaattgacct ttgaccagca aaattatttg attggtgctg cagtgattag cgagcaagca
     5221 gacgaattga tcgatgattt gacactcgtc atcaatcaaa aattgactaa aaaagagttg
     5281 gatagctata tcatggggta tccgacattg gcaagtgatc tctcctattt attgaaataa
     5341 tcagcatgat aactaaagcg cttgttgctt tttccttctt gtgctacact ttaaaaaatg
     5401 aatacctgtt tctggggatg tcgtagacga catgagagta agttgtgaac ttagacccat
     5461 tgaacctgtc agttaatact ggtgaaggga gaaacatagc agacgcttga agcgggttac
     5521 tatgtcgtgc ccatttcaag cgttttttgt tattcatatt agaagaaagg aggtaacaaa
     5581 atgaagaaaa atctttatcc agcagtcagt atggcattca ttttaattat ttggcagatt
     5641 gttgtcatag tttggaaaat acctgccttt attttacccg gacctgctgc tgtattttct
     5701 gcattagcgg cagatgcaca agtattactt tcccattctg tcacgacatt aaatgaagca
     5761 gtgcttggtt tggtaatagc tagcgttctt tcttttatta ctgccttggc aatggattat
     5821 tggaagtttt tagaaatgtc tttttatccg ttactagtga tttcacaaat gttgccaatc
     5881 atggtgttag ggccattact taccctatgg tttggtttcg gtatgcttcc aaaagtgatc
     5941 cttgttgtct tgataagcta ttttccgatc gtagtttctt tttcggatac attgaggaaa
     6001 gtatcaaaag agcaaatcat ctttttgaaa acgatggggg cagatacagg gaagatttat
     6061 cgaatatata aaataccagc gggtttatcc ggcttctttt ccggtttaaa agtggctgct
     6121 acttattgtg tcagcggagc agtcgttgga gaatggctaa gcgctcaagc aggcttaggg
     6181 tattacatga tacgcgtaaa aaacagctac cagatcgata aagtttttgc tgcgatcatt
     6241 tgtgtcattc tattgagttt attgctgaat ggcgcttgtt cattattgaa aaaagggtat
     6301 tatcgtatgc tttatatttt ttaagagaag gggatcaaat gaaaaaatta tggcttttat
     6361 taccgctact gttgctttta tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag
     6421 aaataaagga tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat actaatcata
     6481 ctggcatcta cctagcaaaa gaaaaaggct attataaaga ggctggttta aatgtccaaa
     6541 tcatcgaacc aggagataat tcgacaagta ttggtctagt aggtgcggac aaagcacaat
     6601 ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc agatggacag aatataccag
     6661 tcaaagcaat tgctacagtg atcaaacata atacttctgg attcgccacc ctatcagaca
     6721 gtaacatcca ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg caaagtccaa
     6781 gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt tcaaaactga
     6841 caatggtagg aagcaatgga gaagggccag aaagcttagg aaaatcctca gatatccaat
     6901 ggtactttga aggttgggat atgatcaaag caaaagaagc gggtattgaa ctaaactata
     6961 tcccattgaa agaattagat gaacggttag attactatac tccggtaatt attacgaatg
     7021 atcaactgat caaaagtgat ccagaattgg tacagtcttt tatggatgcc acgaaaaaag
     7081 gctatcagga agctattaag gatccaaatg acagtgctaa actgttgcaa aaatatgcaa
     7141 aagaaaatga tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa aattatacgg
     7201 atgatcctga gaattgggga ttgatggaag aaaaagtttg gcgcaattat acagctttta
     7261 tgcaggaaaa cggattgatc aagcaagatg taccttcgca agaactattc actaatcagt
     7321 ttataaaata ggagtgatag atatgtcaat ccaaatcagc cacttgaatg tagtcattga
     7381 aaacctgaaa atattagaag acatccattt cactatccct aaagatacct ttacatcgat
     7441 tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa actttaacag gagtccagcc
     7501 tatgacttct ggaacgatca aagtagacga tcaaaaagta actggtctcc atacagcatt
     7561 tagttatatg ccgcaagaag atatgttact tccttggctg agcgtctatc aaaacgttac
     7621 gttatatcaa aaaatcaatc atctaacaat caatgaagaa caagtaaggg attatctgga
     7681 tatctttggg ttaaacggct atgaacattt tttgccggaa caattatctg gcgggatgaa
     7741 gcagcgaacg gccttgttac gaacaatcat gaatccttct tcttacttgc ttcttgatga
     7801 accatttgga gcgttagatg ctatgaccag agggcagatg caagactgga tgttgaagct
     7861 gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat gatatcgaag aagcaattta
     7921 tttatcagac aggattcttg tgctttccgc ccgtccggca catgtgattg ctgagattca
     7981 agtgcctgaa aaacgacggt cacgtgaatg gctactgcag caatcagagt tgaagcaaac
     8041 gatttatcag ttattggcag gtgaattaga tgttaagtga tgctcattgt catttagatg
     8101 ggagtcagag tttagcactc cttcagcagg aacacacgat tttgacgatc atcaattgtg
     8161 attcgccaga agaatggaag gaaaacagac agcttgcagc aagtaagact caagccttaa
     8221 gttacggtat ccatccttgg aaagcagata gctatacgtt tgaacaagtt gagccttttt
     8281 tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa tatttggacg aatgtaccga
     8341 tgactacaca aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac
     8401 ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc
     8461 ctaatcacta tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt
     8521 taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag
     8581 ctaaagcagt cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt
     8641 gggctttagg ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa
     8701 agacaatcgc tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa
     8761 acttggaacg tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc
     8821 acctcttttt ggctttcttt gtataaaagt aaactgtttg attaataaat gaatcttagt
     8881 accaaccgtt tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa
     8941 catattgttc tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag
     9001 aatctaattg gtaacgtccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt
     9061 ctttttgtgc gatccattct tttgcagagc ttgtgcttgc tggagtagca gcagtttctg
     9121 ttacagcagg agcaggttgt gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg
     9181 tttcttgtac aacaggttgt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt
     9241 cagcagctgc ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAGCXG010000095         9241 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGCXG010000095 DAGCXG010000000
VERSION     DAGCXG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.098"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="MB022_002254"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="MB022_002254"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBT4467233.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="MB022_002255"
     CDS             complement(2262..2513)
                     /locus_tag="MB022_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBT4467234.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="MB022_002256"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="MB022_002256"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBT4467235.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="MB022_002257"
     CDS             3463..3681
                     /locus_tag="MB022_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467236.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="MB022_002258"
     CDS             complement(3970..4482)
                     /locus_tag="MB022_002258"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBT4467237.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="MB022_002259"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="MB022_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBT4467238.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPHAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="MB022_002260"
     CDS             complement(6935..7378)
                     /locus_tag="MB022_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBT4467239.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="MB022_002261"
     CDS             complement(7379..8167)
                     /locus_tag="MB022_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4467240.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="MB022_002262"
     CDS             complement(8164..9105)
                     /locus_tag="MB022_002262"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBT4467241.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
BASE COUNT         2538 a         2053 c         1642 g         3008 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcatgcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 a
//
LOCUS       DAGCXG010000096         9034 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGCXG010000096 DAGCXG010000000
VERSION     DAGCXG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.099"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            3..1190
                     /locus_tag="MB022_002263"
     CDS             3..1190
                     /locus_tag="MB022_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBT4467242.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1171..2475
                     /locus_tag="MB022_002264"
     CDS             1171..2475
                     /locus_tag="MB022_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4467243.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2501..3514
                     /gene="citC"
                     /locus_tag="MB022_002265"
     CDS             2501..3514
                     /gene="citC"
                     /locus_tag="MB022_002265"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBT4467244.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3518..3811
                     /gene="citD"
                     /locus_tag="MB022_002266"
     CDS             3518..3811
                     /gene="citD"
                     /locus_tag="MB022_002266"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBT4467245.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3816..4721
                     /gene="citE"
                     /locus_tag="MB022_002267"
     CDS             3816..4721
                     /gene="citE"
                     /locus_tag="MB022_002267"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBT4467246.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4714..6273
                     /gene="citF"
                     /locus_tag="MB022_002268"
     CDS             4714..6273
                     /gene="citF"
                     /locus_tag="MB022_002268"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBT4467247.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6270..6827
                     /gene="citX"
                     /locus_tag="MB022_002269"
     CDS             6270..6827
                     /gene="citX"
                     /locus_tag="MB022_002269"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBT4467248.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6820..7683
                     /gene="citG"
                     /locus_tag="MB022_002270"
     CDS             6820..7683
                     /gene="citG"
                     /locus_tag="MB022_002270"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBT4467249.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7917..8326
                     /gene="tnpA"
                     /locus_tag="MB022_002271"
                     /pseudo
     CDS             7917..8326
                     /gene="tnpA"
                     /locus_tag="MB022_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3147 a         1411 c         1831 g         2645 t
ORIGIN      
        1 gtatgcttga tgaagtttta gaaatccata aaaaaaatgt aggtgtttta cggatggaaa
       61 caacagaagc cgtgcaagat gatcaagatt tagcaaaaat gtacacccca ggtgtagcag
      121 aactaagttt aatgattgag aaaaatcatg ctttggcaag agaattaaca attagtggta
      181 aattagtggc cgttattaca gacgggtcgg cggtattagg tcttggaaat gtaggaaacc
      241 aagctggatt accaatcgtc gaagggaaag cgctgcttta taaaaattta gcaggtgtca
      301 atgcgattcc attagccatc gaacaaaaat cagtagacga gattgtacaa acaattgtca
      361 atttgcaaaa tagttttgca gggatccatt tagaagatat tgcagcaccg aagtgttttg
      421 agattgaaga aaaattacaa gaaaaacttt ctattcctgt gtatcatgac gatcaagaag
      481 gaacagcgat agttgtttta gctggattga tcaatgctgc taagatccaa aggaaaacat
      541 taactgaact tcgagttttg atcaacggca tgggagcatc tggggttgca acagctagac
      601 tattaatcgc tgctggtatc aaaaatctaa cattggtgga taaacaaggg agattaaaga
      661 agcaagatag tacattaaat aaatatcaaa gagagttgtt ggatcatata catgatacaa
      721 ataataatgc aagcgttttg gaagatgcaa tcgttggaca agatgttttt atcggtcttt
      781 caataggaaa tgttttaacg cctgaaatga ttggaaaaat gaatgataat ccgattattt
      841 ttgctttagc caatccagta ccagagatca cacccgattt agcaaaagaa tctggtgcaa
      901 agctaatcgc tacaggttct tcgaaatatc ctaaccaagt aaacaatatt ttagtatttc
      961 ccggattgtt caagggacta ttactatcga aagcgaataa agtcgatctt tcattacaac
     1021 ttgaagtagc caaacaatta gctgctaagg tcgcagagcc tacggtagaa aaattcatcc
     1081 ctaatgtttt tgatgaagga gtcgcaaacg tcgtttcaca ggcagttttg gattatttac
     1141 aaaaactaaa agaaaacgag gataatatac atgctgaaaa cattaaatag aattaaaatt
     1201 ggttttattc ctttatctct ttatatcatt ttggtactcg cgtatcttgc aatgacgata
     1261 agcaatatcg ttccagaaaa tgatatgtta gtttctttgg gtattatgac tgtttttgct
     1321 tatttattag aagaaattgg taaaactatt cctattttaa aagatttagg tggtaaagta
     1381 ttagttgtta catttttgcc tgcatttcta gtctataaac attggctacc tcaaagtact
     1441 gtaactgtgg tttctgattt tatgaataat aacaatttct tgatgttctt tatcacacta
     1501 ctagttgtcg gcagtattgc ttcaatgaat cggcaaacat tggttaaagc aacttcacgt
     1561 attatcatta ttttaatttt atgcgatgtg attggttctt tgataggaac aggtgtggga
     1621 atactccttg ggctatctgc ttggaatgcg tatttcttta tcgttgcgcc gattatggct
     1681 ggtggtgttg gggaaggagc gatgccatta tcgattggtt atgcagcaat attatctggt
     1741 atgggacaaa atgatatctt tgggaagatt ctaccgtgtg tgatgttggg aagtttagtc
     1801 gcagttctac tgtctggtat gctcaaaaaa attggagaga aatacccaca attaaatggt
     1861 cagggtaatt tgatcgatgg ggatgatgaa catttagaga acttgaaggg ttcagctaaa
     1921 ccagtcaata ttgaaaaaac attgttagca ggcgtggtag caataacgat ttattttgtt
     1981 gctgtctatg ttgagcactt attaaaatca ctccttggct tttctattcc ttcacctgtg
     2041 atcttattag tctctcttat gggggctaaa atgcttggtt ttatccctaa aaatatcgaa
     2101 gaaggtggaa attcgttgta tggattcact gttcgtggga tcacacctcc cttacttttt
     2161 ggcgtagggg ttgcaaaaac agattggtta gagctgatcg aagtatttaa aaatcctgca
     2221 acgatagtaa caatcattgt gacggtagta gctattgtcg ctacagcttt tatcgccgca
     2281 aaatttttaa aaatgtaccc agttgatact gccatcgcag tttcatgttg tagcggacaa
     2341 ggcggtacag gtgctttagc tatcttagct gcgggtgatc gcatggaatt aatgccattt
     2401 gcacaagttg ctgttcgact agggggtgct atgaccgtaa cgttcgctat ctttttaatg
     2461 ggtctgctat cataagaaca taaagaaagg acgtgaccat gtgtttgatc aaacagatat
     2521 ccaacgatta tttttaaaaa ataactatat caaagaccaa tgggaaaatc ttttgcttga
     2581 ttcaggaata caaaagaagc aaatcgaaga ttttcaacat ttagatcaaa cattgggtat
     2641 ataccataaa gaaaagttag taggaacagt atcttatcaa aacaacgtga ttaaatatat
     2701 cgccgtgtct gaaaaatata aagatggtgg acatacattt aatgtactag tctctgcaat
     2761 aattcaggca atgagtcaac aagagatttt tcattatatt gttttcacga aaccgcgcta
     2821 tcaaaaaagt tttaactttt taggttttga aacaatcgta tctaccccaa aaatagctgt
     2881 attagaaatg ggagatagtt ctataaaaca gtacctagag aggattccta aagtgaatgg
     2941 tgaaaaaaaa gcagcaattg tcatgaatgc gaacccattt acagaagggc attattattt
     3001 agtaaaacaa gctgccaata acaacgattg ggtatatgta tttgtactcg aaaaagaaca
     3061 aaatcttttt tcgacgaagg accgactcaa attagttcaa caaggattac aaaaactgaa
     3121 aaatgtcatt gttctatcag gtggcgagta tattattagc cctgcaacgt ttcccaccta
     3181 ttttttacga gaagatgatg aaatagcaaa ggagcaaatg gtcgtagatg ctactttatt
     3241 caaagaaaga attgccactt atttagatat ctctgctaga tatgtgggag aagaaccact
     3301 ttccccaatg actcgttcat ataacgacat actggaaacg attttacccc ctgaaataaa
     3361 agttcatgtc cttgcaagga agaaaacgga acaacaccaa gttatttctg cttctcaagt
     3421 tcgtaaagcg tatcttgctg ggcaattgga aaaaataaaa tatatggttc cagaaacaac
     3481 ctatcaatac ttaaaaaata aaagggagcg ttaagtaatg gaaataaata aatcagcgat
     3541 tgctggaaca ttagagtcaa gtgatgtcca aatcatgatt tcaccgaata cagaaggaat
     3601 agaaatcgat ttagacagtc aagtgattga gcaatttgga aaacaaatca aaaaagttat
     3661 ctatgacaca ctagcagttt atcagattaa aaacgtaaaa ctcaaggtag tagataaagg
     3721 ggcattagat tgcgttattg ttgcacgatt aaaaacagcg atccatcgtt ccttaggtat
     3781 ccaacaccaa gaaacaccgt gggaggttta attagatgat gaaagaacgt ttgagaagaa
     3841 caatgatgtt tgttccagga aataatccag cgatgatcaa agatgcaggt atttatggtg
     3901 ctgactcgat tatgttcgac ctggaagatg cagtgtctat ggcagaaaaa gacagcgcaa
     3961 gaatgcttgt ggctgaggca ttgaaaacag tggattatgg tgacacagaa ttaattgttc
     4021 gaataaatga cctgcactca gaatttggac gtgcagacat ttttgcaatg gttaaagccg
     4081 gcgcgcatgt attacgttta cctaaaacag aaacctctga agatataaca gaagtagaaa
     4141 aagtgattat agaagcagaa aactatcata atgttccgat agggcgaaca ttgatgatgg
     4201 cagccattga aggtgcttct ggtgtattga acgcacctgc gatcgccaaa gcatctgatc
     4261 gattgattgg aatcgccatt ggggcagaag actatgtgac aaatatgaaa accaaacgat
     4321 atccagatgg aaacgaattg ttttttgctc gcagtatgat cgttcatgct gcacgtgcag
     4381 caggcatttc cgcatttgac actgtttact ctgatgtgga tgatatggaa ggatttttaa
     4441 acgaagtacg tttaatccat caattaggtt ttgatggaaa atcttgtatc aatccacggc
     4501 aaattccagt agtcaatact gtttatcagc cgtcaatcaa agaaatcaaa cgatcactag
     4561 acatcatctc ggcaatcgaa gaagcaaaag aaaaaggttc tggcgtaatt tctttaaatg
     4621 gaaaaatgat cgataaacca attgtagaac gtgcacaacg gacattgatg ttagcaaaag
     4681 catcacgatt gattgatgag gagggaaata tccatgcata ataaacaaag acgtagcatt
     4741 ccatcaagtg ttttaacaaa gtataaaaaa gaattccaaa gtgtttcatt atcgccacaa
     4801 acgattcaac gttgtgcacc aaaagtaacg actgtaacta atcagacaaa taaattgatg
     4861 cactcgatca aagaagctat agaaaaatcg ggcttaaaag atggaatgac gatttcattt
     4921 catcatcatt tgagagaagg tgattttgtt tttaaccaag tgatggccgt gatccgtgaa
     4981 atgggcatca aagaattgac cttagcacca agttctttaa cgaacactat gaatgagatg
     5041 gttattgaat gcatcaagga cggtacagta actcatatca caagcagtgg gatgcgcggc
     5101 agcttaggtg aatttatttc tcatggagga atggacaatc ctgtgatttt acgttctcat
     5161 ggtaatcgtc cacgtgcaat tgaaagtggt gaattgaaaa tcgatgttgc atttctagga
     5221 gtaccaacat ctgatgatta tggaaatgct aatgcgactg ttggaaaatc cgtttttggt
     5281 tcattagggt atgcaaaagt agatgcacaa tatgcagata aagtcattct tctaactgat
     5341 catttagttc cttaccctaa tacgcccatt agtattccgc aaacacaagt cgactatgtc
     5401 gtggaagtag aagcaattgg tgatccgaat aaaatcggtg ctggtgctac acggtttact
     5461 aaaaacccaa aagaattaaa aattgctgaa atggtcaaag atgtcattgt tcactcacct
     5521 tatttcaaag atggcttttc ttttcaaaca ggaacgggtg gtgcagcttt agcagtaacg
     5581 cgttacttag ctagtgaaat ggaagaaaaa cagatcaaag cttcttttgc attaggtggc
     5641 atcactaaac caacggttga tttattggaa aaaggtcttg ttgaaaaagt gttagatgtc
     5701 caagactttg acaaaggagc agctgaagga atggcgcggc ttgagaacca acaggagatc
     5761 gatgcttcct tttatgctga tccaagtaat aaaggagcgg ttgttaacca attagatgtc
     5821 gctattttat ctgcattaga aattgacaca aacttcaatg taaatgttat gactggttca
     5881 gatggtgtgc taagaggtgc gattggtgga catcaagatg cagcaacggc taaacttact
     5941 atcatctcag cgccgttagt acgtggccga atacctacag tagtaaatga tgttactaca
     6001 ttgatcacac cagggaaaag tatcgatgta ttagtcacag aagtaggggt tgctatcaat
     6061 ccccaacgta aagatctaat agctattttt gaaagaattc cacaaattcc tgtttttaca
     6121 atagaagaat tacagcaaaa agcagaaaaa atagtaggca tcccggaacc tttgcaattc
     6181 acagatcgga tagttgccta tgtagaatac cgagatggtt caattttaga tgtcgttcgt
     6241 caggtgaaag aatttgaaga agagagatca tgatgtttga tactggagaa attcaagaaa
     6301 tcgaggatat tttgatccat aaagaacaac gtgctaactt gcaaagcaac ctgctttcca
     6361 agtattcaaa tagttcattg atggtcttta cattgaatat tcctgggccg atcaaaaata
     6421 atccaatgat tgagcaggta tttcgtcagg gacagcagtc aattcagcaa atgtttttaa
     6481 aaaaaaaggt ggaaatgacg tacacaaaag aaatcaattt gccaagtgga ttggactggt
     6541 acgtaatagt aacagaagca cctttgaatc tcaaaaaatg gttggtcacg ttagaagagc
     6601 aagacgcatt aggtagactt tttgatttag atgtcttgtt caaaaaagga gagcagataa
     6661 caattgtcag tcgccaagct cttaatcatg gggaacgtcc ttgttttatt tgtgggaaat
     6721 cagcaaaggt atgtgcacgt aatcgtacac atactgtgaa agaaatgcaa aagaaaatcg
     6781 aacaaatagt agccgattcc ttaaaaggag cattgaaaca tgcatgataa tattcaaaaa
     6841 atagcacaag aagcacaatt agcgatgctt tatgaagtta catgtatcaa taaaccagga
     6901 ttggttgatc cagtagaccc cgggtctcat caagatatgg acatttttac ttttctacaa
     6961 agtagtgtag ttttgactcc ctattttgaa gaatttatcc aaacaggatt ggaccgacaa
     7021 catcaaccaa ttgaagaaac ctttatggcg attcgacaga ttggtttaga ggctgaaact
     7081 gcgatgttct cagtgacaaa tggtataaat acacataaag gtgctatctt ttcattgggg
     7141 atttttcttg ctatttgtgg ccggttaact atttggaaag ttccttgtaa aaaaagtttc
     7201 tttcaaaaag aaattcaaaa aatgacgaaa aatctactta acgattttgg gaagattgat
     7261 caaacaaaac caaaagcatg gacttgggga gaatatctat ttgttaacca tggattgaca
     7321 gggatacgag gggaagccaa aagaggttat ccttgtgttt ttgaccgagg attgccttat
     7381 tatcaagcat atcaagggtc gcaacaagat aagatgatag acactttact ttttctatca
     7441 atggaagtag aagataccaa tttgatcaaa cgaagtaata atagacatgt ttttgaagag
     7501 tatcaattac ttattcgtcc ttattttgaa ttagggggtg taaaaaccat acaaggtaaa
     7561 aaatatttgg attacctaaa tcagcaattt aaaaagaaaa attggagtat tggtggaagt
     7621 gcagacttat tgatccttac aatcttttta gataaaatca tggacaaagg atggttatgt
     7681 tgatactata atacattatt ttgtattagt aagctaataa ctaaaaaatg gttcaataca
     7741 aatattttat aaaaaatggt agtgcctatc ctttcagatt gtagacaaag aaaaatttct
     7801 ttgtctacaa tttttttgtt aatgacttca gtcagtttcg tgcgcaaagc gtgcgaaaca
     7861 agaaaaccat gcgtttctac acgtggaaga actagcatat cgaaaggagt tttttcatgt
     7921 caaatgacga taaaagttta gcccatactc gttggaattt taagtatcat attgtattta
     7981 caccaaaata tcgacgaaaa gtaatctata ggcaattacg aaaagatatt ggaaaaatac
     8041 ttaggaagtt gtgcaaaata aaaaacgtgg aaattataga agcacatgca acgccagatc
     8101 atattcacat gcttgttcaa gtaccatcaa aaataagtat atccagtttt atagttatct
     8161 gaaagatcgt agtgctgtgc taatccacga gcagcacgta aatcacaaat atagatacgg
     8221 aaataaatca ttttggtcgg aggaatatta tgtgagtaca gtcgaattga atcaaaagac
     8281 aattgaaaaa tacatccgtg aacaagaagc tgatgatcgc atttgagata gtataagtaa
     8341 aagagagtag atgttgcaat aacaaaaggg taaaaatgga ctacctccag taaaataccg
     8401 aaaaatagtt taacttttgg agataagtcc atttaagggt ctatagaatg acaatttttt
     8461 taggggattt tactattatt acttcaagac ccgcttggat tcgcaaagtg agtgaactct
     8521 taaaactacc tgatagtagg ttgttttaag agttatctga aagtaaaaat tgcatttata
     8581 tttttgataa atacgtaaat ttatatatac gtatttatca aagttttagg ccaaaggaat
     8641 attgtgtaaa tacactagga ttgaatgaat agaccagaag aaattgcgtc caagaacaaa
     8701 agaagcatga tagtgctcta tataaattgg gcattcaaga atctgaaatt taaaggtaga
     8761 attttagtta gacttttaga ttttaaacac aaaaataacc atacaagcaa caagaagcaa
     8821 agtgctatat tataaattgt ccgtccgttt acttacataa gatcattggg gctattttta
     8881 aaagagaata atagataata aatgaaaaaa aagtcaagtc cacaatcctg tgtaaaatac
     8941 tatacaatgc tctcactatt ttttacttgg caaatttaat atattttctt gatgaagaaa
     9001 gccactcatc gtgttgatca ataaggactg ctcc
//
LOCUS       DAGCXG010000097         8835 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGCXG010000097 DAGCXG010000000
VERSION     DAGCXG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.100"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(229..2121)
                     /locus_tag="MB022_002272"
     CDS             complement(229..2121)
                     /locus_tag="MB022_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467250.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2118..3845)
                     /locus_tag="MB022_002273"
     CDS             complement(2118..3845)
                     /locus_tag="MB022_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467251.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3861..4511)
                     /locus_tag="MB022_002274"
     CDS             complement(3861..4511)
                     /locus_tag="MB022_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4467252.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4715..5011
                     /locus_tag="MB022_002275"
     CDS             4715..5011
                     /locus_tag="MB022_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4467253.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5183..5803
                     /locus_tag="MB022_002276"
     CDS             5183..5803
                     /locus_tag="MB022_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBT4467254.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5895..7061)
                     /locus_tag="MB022_002277"
     CDS             complement(5895..7061)
                     /locus_tag="MB022_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4467255.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7073..7426)
                     /locus_tag="MB022_002278"
     CDS             complement(7073..7426)
                     /locus_tag="MB022_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4467256.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7554..8576
                     /locus_tag="MB022_002279"
     CDS             7554..8576
                     /locus_tag="MB022_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBT4467257.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8595..8756
                     /locus_tag="MB022_002280"
     CDS             8595..8756
                     /locus_tag="MB022_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467258.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
BASE COUNT         2686 a         1815 c         1606 g         2728 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg
       61 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      121 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      181 cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc
      241 ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt
      301 atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc
      361 gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt
      421 catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag
      481 gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc
      541 ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg
      601 aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc
      661 gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag
      721 taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc
      781 gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt
      841 cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt
      901 aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac
      961 tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc
     1021 atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa
     1081 acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac
     1141 atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac
     1201 atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc
     1261 tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc
     1321 agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt
     1381 caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat
     1441 catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca attgccagct
     1501 gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt
     1561 caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat
     1621 atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa
     1681 atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa
     1741 aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat
     1801 tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc
     1861 agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat
     1921 ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt
     1981 agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt
     2041 tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg
     2101 acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct
     2161 tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct
     2221 tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa
     2281 acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg
     2341 aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc
     2401 cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca
     2461 tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat
     2521 gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc
     2581 gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat
     2641 tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat
     2701 aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg
     2761 acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca
     2821 tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact
     2881 gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg
     2941 tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg
     3001 atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata
     3061 tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat
     3121 gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa
     3181 tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc
     3241 actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc
     3301 atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact
     3361 gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc
     3421 gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt
     3481 tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca
     3541 aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc
     3601 agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc
     3661 aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct
     3721 acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg
     3781 atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt
     3841 agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa
     3901 ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac
     3961 gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca
     4021 tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct
     4081 tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact
     4141 tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg
     4201 ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca
     4261 ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca
     4321 aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg
     4381 atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac
     4441 tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc
     4501 tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt
     4561 cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc
     4621 gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt
     4681 aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga
     4741 cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga
     4801 ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca
     4861 attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag gcaggcgatc
     4921 agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg
     4981 gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg
     5041 tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt
     5101 caaccttatc aatatgactt gatagccttt tctttttctc atagttataa caatcatagc
     5161 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5221 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5281 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5341 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5401 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5461 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5521 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5581 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5641 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5701 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     5761 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     5821 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca
     5881 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     5941 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6001 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6061 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6121 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6181 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6241 ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6301 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6361 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6421 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6481 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6541 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6601 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6661 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6721 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     6781 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     6841 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     6901 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     6961 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7021 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7081 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7141 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7201 ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7261 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7321 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7381 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7441 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7501 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7561 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7621 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7681 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7741 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     7801 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     7861 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     7921 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     7981 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8041 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8101 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8161 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8221 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8281 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8341 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8401 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8461 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8521 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8581 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8641 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8701 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     8761 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     8821 tttctacaaa taaaa
//
LOCUS       DAGCXG010000098         8499 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGCXG010000098 DAGCXG010000000
VERSION     DAGCXG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.101"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(188..865)
                     /gene="nth"
                     /locus_tag="MB022_002281"
     CDS             complement(188..865)
                     /gene="nth"
                     /locus_tag="MB022_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBT4467259.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(872..1570)
                     /locus_tag="MB022_002282"
     CDS             complement(872..1570)
                     /locus_tag="MB022_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4467260.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1838..3022
                     /locus_tag="MB022_002283"
     CDS             1838..3022
                     /locus_tag="MB022_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBT4467261.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3028..3471)
                     /locus_tag="MB022_002284"
     CDS             complement(3028..3471)
                     /locus_tag="MB022_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4467262.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3697..3984)
                     /locus_tag="MB022_002285"
     CDS             complement(3697..3984)
                     /locus_tag="MB022_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467263.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4155..5507)
                     /locus_tag="MB022_002286"
     CDS             complement(4155..5507)
                     /locus_tag="MB022_002286"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBT4467264.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5780..6226)
                     /locus_tag="MB022_002287"
     CDS             complement(5780..6226)
                     /locus_tag="MB022_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4467265.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6462..7388)
                     /gene="cysK"
                     /locus_tag="MB022_002288"
     CDS             complement(6462..7388)
                     /gene="cysK"
                     /locus_tag="MB022_002288"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBT4467266.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7528..8415)
                     /locus_tag="MB022_002289"
     CDS             complement(7528..8415)
                     /locus_tag="MB022_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4467267.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2312 a         1749 c         1411 g         3027 t
ORIGIN      
        1 tctacatcgg gtattttccc aataaaaata acaactttta tttttatcgc aataaattgt
       61 aataagttac catcctagct actgagtatg tacatttact ttttcatgca aaaaagataa
      121 acgaaacagc ttttctccat aataacaaga taattcatac atttatttgc aattaaagaa
      181 accacgtcta caggcgtggt ttctttttta gcgccttttc ttttagacgc atccgatttt
      241 tcccatcttg acatattgat agcaaaggac atacctcgca cttgggattt cttgccgtgc
      301 aatgatatct accaaaaaaa atcagtgtat gatgtgtctt tacccataat tcttgtggta
      361 ctttccgcat cagtgtttct tctacttcca tgaccgtagc atctagtttg cagatcctca
      421 ggcgttttga gacacgttcc acgtgggtat ctactgcgat tgctggtatc ccgaatgcat
      481 cccccaatac gacatttgct gtctttctgc ctacaccagg aagagacatc aattcttctc
      541 ttgacgttgg cacttgccca tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt
      601 tttttgcttt atttcgatac aaaccaatcg tttttatttt aagaatgatt tcatcgattg
      661 aagcttctgc taacgcatcg ggtgtcggaa aagaggcaaa caaatctggc gttgctttat
      721 tcactgatac atctgtcgcc tgggcactta agatcactgc aatcaataac tcaaatggat
      781 tattgtgctt gagttctcca tgtgcttcgg gaaacattcc atacatcgtt tctagagctt
      841 ccattgtttt ttgtttgctt aacatattcc gctactcctc caaccaattt ttcagtgaga
      901 ctttcggcaa ttcttcttgt tgagctgact gttctacttc tctttgaaga agctgctgtt
      961 ttcttcgttt ttggtcttct tccacttgtt gttttgtacg tagatttttt ctttcccacg
     1021 atagcaaaat gcggtcaacg taattaaaac tataagcctg atttaatacg gcttcccgca
     1081 gtgctagctt caaaatttcc ggctgataat ggtcttcttc tagccattgt ccgatccgct
     1141 ggaactcgat cgaagaaagc gggcgtccaa attcttgttc aaataattga tacactgact
     1201 ggatttcttt ttcttgtgtt ttttcttcgt attttttttc ttgtgttttt aaatactcac
     1261 ctaatagatc atagatgggg taaagattat atcgatctgc ttttttaccg ttgacaatcg
     1321 tcgtttctat cttgataaat ccatttgtta cgagacgatc cagtatttga aaaatctgat
     1381 cttgtttgat tcccatatcc atagcaatca tctgcaaatc agggaaagta tctccttcaa
     1441 gctgtgccat atgcaactgt agaacaaata aaaattcttc agattgtaga ccgatcctat
     1501 gataattttt aagtaaaaga ttagatatga ctgtttgtcc agcgcttaag taattttcta
     1561 aattcaacat gatttctcct ccgctcaatc acagtatacg taaaaaaaac atgggaagca
     1621 agaacaggtt tcttttctat ttacttcact gatcggtcag atttacaatg tcgtctactt
     1681 gatttttgtt tgttcatgtt tttgatgcgt atcttcactt ttcggaataa ataaattaag
     1741 tagatttcgc tgttccttag aatgttgaca aggaaacaaa attactatac actatttttg
     1801 tttccaagtt aacattattg cataaaggag tttttacatg tacagtttca caaataaaca
     1861 ttctaaagtt tttggatacg tctctgtctt attcactgga ttaggattta ctatcgtcag
     1921 tcctgtactt ccgtttttaa cgctgcctta ttctcactcc atacatcagc aagctttcta
     1981 tatcactttg ctcatgtctg tttatgctct agctgccttc ttatctgccc ccattctcgg
     2041 tagcttaagt gatcattttg ggcgtcatcc gatccttatt atcagtttat taggatcaag
     2101 tatcggatat ttgctttttg gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat
     2161 cattgaagga ttgacggcag gagaaatctc caccttatat gcttactttg ccgacatcac
     2221 ggaacctaat gaaagaacca aagtttttgg atggatgggt gctttagtcg gtataggaac
     2281 aacgctgggt cctattatag ggggtctatt agctgagttg gggaattctg ttcctatatt
     2341 tattggagca ctctttactt tcttaaatgc cgtttatggc tacacattta tgctagaaag
     2401 ccttccaata aaaaaacgtt cagtagattt aagtttctcc catgtaagac cttttcacca
     2461 attacaacaa ttattcaaga tttcatccgt tattccttta ttgacagcag gttttgctgt
     2521 ttggttggca gctggctctc ttcaatctat cttttctcaa ttttcgattg acgcctttca
     2581 gtggaaggct gggttggttg gtttaagctt ctctttgatt ggaatcttgg atactgtctc
     2641 gcaattgttc attatgcctc gattgctcaa aaaattttca gaacagcaga tcacaagaat
     2701 agggatgttc agtgaaatcc tcgcctatct attcatcaca ttatctggaa ttcttctact
     2761 gccgattttg tttttatttg ggattatctg ttatggtttc ggtgattcta tttttactcc
     2821 tgtattcaat ggccaactat ccaattctgt ctcagataac cagcaaggtc ttgtaatggg
     2881 cggtacccaa agtatacaat ctctatcgcg cgtcatcggc ccgctaatcg caggacagct
     2941 ttatgctgca gcaccatttc ttccgacggt attcggattt ttgcttatga tttttgccta
     3001 ttatcactac aataaaaaat agtagcttca tttgctactt cgtataaatt ctctgtatcg
     3061 accgataaaa cgaaatactg tgtcaaattc ttcttttgac atattttcaa atacagtagc
     3121 atctcgctgt acccattttt gatggagttg ctcgtgtcta ttccttattt tttgtccgat
     3181 tggcgtcaat tgaaaataga tttctttttt attctcttcg ctttgataag tgtttatgat
     3241 ccctctagct atcagttttt ttgttagctt actgatggct cctctagtca tgtaactagc
     3301 attcgctaat tttgtcacgt taggatcagg gattttcccg atatgttcaa tcacttctag
     3361 ttcatttaga gtaaatcctg caaattcttt ttccatttct ggtttattac gatgaataat
     3421 ctcattagct aaatccagat attcttctat tagcagttgt cttttatcca tttctacacc
     3481 ttctttttct ttcattatat tgtttttttg tttcctattc aacaaagata aaaatatttt
     3541 gtaggaaaac ccaatctgaa accaagaaaa aaaggggttg acaaagcttg tcataacccc
     3601 tttttctgat taaaagatag taaagagaca gtcatcggta attgtgatga ttttttgtca
     3661 tcacttaagg atgagcggcc ttttcaaaaa ctgtctttaa agatcatcat cagtttctag
     3721 cacatatggc tgaccttcat atgtgtactc tgtattcaat gtgccgtcat tttcatctga
     3781 gtagatgcgt ctactgaaag gaaaacgatg agaaacatta tctttggcgt catttgctgg
     3841 catcgtgtaa tattcctctt gcgtttcatt caactgagag agtacttgat ctgccaactc
     3901 atcaatattg atcgttaatt tcttcgaagc acttttataa tcttctaaaa atgcatcttg
     3961 ttggacaaaa tattctttat tcatcttctt tctcaccttt cttttcaaaa tcaaataaag
     4021 ctgcttgttc ttttatgtaa cagctttatc tttgctgctt attgtgacaa aaaattcgaa
     4081 gaaagacaac taattgtact tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc
     4141 acaacccttt ttctttattg tgcagtttcg atttcgccta gcgctgcttg tgcaagtagg
     4201 ttcaagtaat tccatgggcg gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga
     4261 tcttctacag tcattttatt ttgaacagct aaagacatgg tatttgctga ttgtgtaatg
     4321 tcgtatttgg acatgaactg agcacctacg atacgattcg ttcctttttc atagactagc
     4381 tccatcatta ctttttcagt agttggcatg aactctggac gataattatc ttcaaataca
     4441 gtcgcttgga cttctagatc gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg
     4501 atcgtccagc caaataggta aagtcctgaa gttccttgag taccacgata cgccatttta
     4561 tgatctgtca agttatttcc cacaagcaaa ccttgacgaa cggcatttgt tgccaaagga
     4621 atatagtttt gcgtgccact tggattgtag ttgactactg cagagtctcc agctgcaaaa
     4681 atgtctggat tacttgtctg catgtattca ttcactttga ttgccccgtt tggcagtgta
     4741 tctacttttc cttcgatcag ttttgtattt ggacggaagc ccacacataa aatgaccata
     4801 tctgtatcaa atgtatctgt tggtgtagct acttgtttca cttttccttc ttcatctgtg
     4861 ataaattcag taacattttc gcctagcgca agagtaacac cgcggtctgt caattctttt
     4921 tctaagatat ctgtgaatgg tttatctaga tatttattca agatgcgatc taatccatca
     4981 atcaatgtca cttgttttcc agattcgaca aaagcttcta ctaattcaat accgatatat
     5041 ccaccaccaa caattactac acgttttgct tctttcgctt gtgcaataat ttcatttgct
     5101 tgattgtagt ttttgcatag gagtacattt ttcgcatcga ttccactaat cggtgggata
     5161 ataggccatg aaccagttgt catcactaat ttatcataag aaaccgtttc tgtttctcct
     5221 gttttcagat cttttgccgt tactgtttta ttttctgtat cgacgtctgt tacatcgtgt
     5281 tccattttaa ctgttgctcc taatgaagat aattcttcag gatttgagta gaacaagcct
     5341 gctgggtcct ttactactcc gccgacataa agtgcgatac cgcaagataa gaaagaaata
     5401 ttgtcatttc tttcatatac tgtcacttct gcttctggat gattttttaa aatacttttt
     5461 acagctgcag tacctgcatg agtacagcca attactacta ctttcattta tggtccctcc
     5521 gattaacatt ttgtccttcg acaaaactac tataacagaa ataaaacgga tacaacagtt
     5581 taatgcttgt gaattcacaa aggtttgttt gtagtttcat tccttttcaa gcataagaac
     5641 ccaatatcta caagattctt attttaataa tattcacttt tatatctaag attgtgaata
     5701 aaagacaaaa aaataaaaaa gctagcgttt tttttacaca gctagctttt tttaggtata
     5761 acacttgtag aaactgtttt tacatttgcg ccttttttgc tgactcttta caatccggac
     5821 agagtccata caattccaac cgatgttcat taatctcaaa atcagtcagt ctgcttgcag
     5881 ccatttctat atcttcaagc cctgggtaat gaaaatcaac aatcttaccg cagttttcac
     5941 aaattacatg ataatgacgc tttgaactga aatcaaatcg gctagaagca tcgccgtact
     6001 tcatttcttg aacaaatcca atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa
     6061 cactcatatt tggaaaccga tgttcaagtg ctttataaat ctcatcagcc gttggatggg
     6121 aatgatgttc aatcaaatat tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct
     6181 ctttttttaa cttttcgata acctcttcga ctaacttatt ttccatgtaa acaccccacg
     6241 ttgttattct cgtcttatca tactattttt acattttaaa atccacttaa catgaaaaga
     6301 aaattcaatt ttattgtatt ttaatgaaaa aagacgtaaa acagtcgcgt tcactattct
     6361 acgtctctat tcaaataatt tttctcatca taaatttttg tgtgttcttt tcatctttaa
     6421 aagtttttta cataaaccta tctggagaaa aaccaagtgc cttatatctc ttggtaaagg
     6481 gctgtagata aataacgttc accattgtcg ggaaccacag ctaatacctt cttgcctttt
     6541 cctaattctt ttgcgacttt tagcgcggca gcaattgcag cgccagatga gatgcctact
     6601 aaaatccctt cttttcgccc aacttctcta gctgtttcca tcgcttcgtc tccactaata
     6661 gaaaggactt tatcatacac agaagtatcc agtgttttag gaacaaaacc tgtaccgatt
     6721 ccttggattt tgtgaggtcc cgcttctttc ccttctaaaa tagcagattc tgctggttct
     6781 accccgatta attcgatttt cggatacaca cgttttaatt ctcggccagc gccagtgatc
     6841 gttcctcctg taccgatacc tgcaacaaac gcgtccaagc catttactcc aaatgcttga
     6901 tgtatctcag gaccagtcgt cttctcatgc acaagaggat ttgcttcatt ttcaaattgt
     6961 aatggtaaaa agtagccgtt ttcttttgcc aaacgttctg cttctctgat tgatccagaa
     7021 ataccatccg caccaggagt caaaatcaac tctgcgccgt accctttcat caataaacgt
     7081 ctttcgatac tcatcgtctc aggcatcacg atgattactt tatatccttt tgcaactcct
     7141 accatcgaca atccgattcc tgtatttcca gaagtaggtt caatgatagt atcacctggt
     7201 ttcagtagac catcgtgttc tgctttttct atcatactta aagcaatacg atcttttaca
     7261 cttcctcctg gattaaaaaa ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg
     7321 attttattta atttgacgat tggcgttttg ccaatcaatt cggtgactga gttatagata
     7381 gctgtcatct tcaattcctc cttgtctttg ctcttcttac ttatcataat acaatctgtt
     7441 catttatggc gagtgattcg tctttgacta gcaaaaaaaa gacaaagata taccatatat
     7501 ggtaatttct ttgccttttt atctaaatca aacgtttagt tcttgaccaa ctaataaaga
     7561 ataactggac atagacatgc catttttcgc cattagatca tcaacagaca tgccaaattt
     7621 ttgtgcaata tcccatagcg tatcacctga cacgactgta tatgtctgac cattatttgt
     7681 atttacatct gttgttgaag atgatgacgt tgaagtcgtc gtagtagtcg ttgttgttgt
     7741 acttgtggtt gttgtagccg aacctgttga tggcgtatca aaacgggtta aattataagt
     7801 attaatcaat gagttcaatt tggccccata atttggatcg gtagcataac gacctgtcaa
     7861 gtacgctgtt gcatcataaa aacttgttgt attgcttttc cacacgccag cataatgata
     7921 atcgcccgtg gaaaaactgg ttgaacgaag aacattcgca tggtcttgga aggattccca
     7981 ataagaagaa tattttcgga atggctcatt catcgttacc cattgtccgt ttacatattc
     8041 tttggttgcc atataaacga cctgtccgtt atagcttcct ttcacaccga atagattata
     8101 atttggcgca ctagctaatc cggaagtgcc atatgcactt tccaacaacg cttgagcgat
     8161 catgactgaa gcatacaagt cattacttgc agcaacttct gacgcagacc agccgatttg
     8221 atcgataaac gcttgaggat cagcgactgt ttgagatcct tgttcatctg catgaacatg
     8281 ttgcggtacc gctgtaacca tcatcggccc aactaaaaga ctcgttccaa ttaaaggagc
     8341 aatcgctttt ttattgatca ccatttttct tttaggtcta tggtgccggg cactgcgggt
     8401 cttcagctct tgcattcaaa ttcctcctca aaataaaaaa acttaataaa aagttctctt
     8461 cttaaaacaa acttaattaa ttaaattata cttttaatt
//
LOCUS       DAGCXG010000099         8463 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGCXG010000099 DAGCXG010000000
VERSION     DAGCXG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.102"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            205..354
                     /locus_tag="MB022_002290"
     CDS             205..354
                     /locus_tag="MB022_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBT4467268.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            365..1879
                     /gene="dltA"
                     /locus_tag="MB022_002291"
     CDS             365..1879
                     /gene="dltA"
                     /locus_tag="MB022_002291"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBT4467269.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1876..3081
                     /gene="dltB"
                     /locus_tag="MB022_002292"
     CDS             1876..3081
                     /gene="dltB"
                     /locus_tag="MB022_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBT4467270.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3126..3359
                     /gene="dltC"
                     /locus_tag="MB022_002293"
     CDS             3126..3359
                     /gene="dltC"
                     /locus_tag="MB022_002293"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBT4467271.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3362..4630
                     /gene="dltD"
                     /locus_tag="MB022_002294"
     CDS             3362..4630
                     /gene="dltD"
                     /locus_tag="MB022_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBT4467272.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4771..5853
                     /locus_tag="MB022_002295"
     CDS             4771..5853
                     /locus_tag="MB022_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBT4467273.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5853..6656
                     /locus_tag="MB022_002296"
     CDS             5853..6656
                     /locus_tag="MB022_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4467274.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6726..7466
                     /locus_tag="MB022_002297"
     CDS             6726..7466
                     /locus_tag="MB022_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4467275.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7477..>8463
                     /locus_tag="MB022_002298"
     CDS             7477..>8463
                     /locus_tag="MB022_002298"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBT4467276.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEEL"
BASE COUNT         2719 a         1391 c         1742 g         2611 t
ORIGIN      
        1 atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa ttataaagaa
       61 aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa
      121 aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt
      181 gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat
      241 tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat
      301 ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa
      361 gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga
      421 tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga
      481 cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg
      541 caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta
      601 tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa
      661 accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat
      721 aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag
      781 cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa
      841 aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt
      901 tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt
      961 attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt
     1021 tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt
     1081 ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt
     1141 gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag
     1201 tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac
     1261 tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc
     1321 gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc
     1381 aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa
     1441 caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac
     1501 aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca
     1561 gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt atctgctcca
     1621 agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca
     1681 gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa
     1741 atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca
     1801 ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc
     1861 tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt
     1921 tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga
     1981 ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat
     2041 tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg
     2101 ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt
     2161 ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca
     2221 tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg
     2281 gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg
     2341 ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag
     2401 ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt
     2461 tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct
     2521 gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg
     2581 tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt
     2641 acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg
     2701 aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat
     2761 gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta
     2821 atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg
     2881 tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg
     2941 atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta
     3001 gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta
     3061 gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa
     3121 ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa
     3181 gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc
     3241 aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc
     3301 gagaggattg gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag
     3361 tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt
     3421 tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc
     3481 ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc
     3541 tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca
     3601 tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc
     3661 cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa
     3721 caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga
     3781 ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa
     3841 agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga
     3901 gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga
     3961 atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag
     4021 caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa
     4081 acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac
     4141 gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc
     4201 agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt
     4261 tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc
     4321 ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc
     4381 caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa
     4441 tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt
     4501 ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta
     4561 tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca
     4621 attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct
     4681 gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca
     4741 caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt
     4801 gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga
     4861 gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc
     4921 aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt
     4981 tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg
     5041 tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc
     5101 cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct
     5161 tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag
     5221 tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc
     5281 agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga
     5341 gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg
     5401 caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt
     5461 gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca
     5521 gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat
     5581 aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat
     5641 acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt
     5701 ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat
     5761 gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt
     5821 gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat
     5881 atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt
     5941 tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc
     6001 cggaagaaag catgcagcag cacttattga gttttctagt cgataattta cttttattcg
     6061 cattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa
     6121 gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt
     6181 tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa
     6241 tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg
     6301 tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg
     6361 tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt
     6421 tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag
     6481 atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag
     6541 caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag
     6601 aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg
     6661 taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat
     6721 gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga
     6781 caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta
     6841 cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc
     6901 attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc
     6961 ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact
     7021 ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat
     7081 taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg
     7141 ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt
     7201 atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc
     7261 tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg
     7321 cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag
     7381 aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg
     7441 cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac
     7501 cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat
     7561 gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat
     7621 acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc
     7681 ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg
     7741 cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca
     7801 cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat
     7861 ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga
     7921 acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac
     7981 aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt
     8041 cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa
     8101 attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg
     8161 aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag
     8221 tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat
     8281 ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta
     8341 ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta
     8401 tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa
     8461 ttg
//
LOCUS       DAGCXG010000100         8456 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGCXG010000100 DAGCXG010000000
VERSION     DAGCXG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.103"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            385..1824
                     /locus_tag="MB022_002299"
                     /pseudo
     CDS             385..1824
                     /locus_tag="MB022_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2169..2336
                     /locus_tag="MB022_002300"
     CDS             2169..2336
                     /locus_tag="MB022_002300"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467277.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            2595..2810
                     /locus_tag="MB022_002301"
     CDS             2595..2810
                     /locus_tag="MB022_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467278.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2998..3285)
                     /locus_tag="MB022_002302"
                     /pseudo
     CDS             complement(2998..3285)
                     /locus_tag="MB022_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3472..4383)
                     /locus_tag="MB022_002303"
     CDS             complement(3472..4383)
                     /locus_tag="MB022_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4467279.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            complement(4402..5406)
                     /locus_tag="MB022_002304"
     CDS             complement(4402..5406)
                     /locus_tag="MB022_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4467280.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(5403..7511)
                     /locus_tag="MB022_002305"
     CDS             complement(5403..7511)
                     /locus_tag="MB022_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4467281.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            complement(7516..8436)
                     /locus_tag="MB022_002306"
     CDS             complement(7516..8436)
                     /locus_tag="MB022_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4467282.1"
                     /translation="MPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2439 a         1710 c         1558 g         2749 t
ORIGIN      
        1 tttagtataa tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa
       61 acaaataggt gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaattttc
      121 ttacgtctca cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga
      181 aattgctaga agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa
      241 gcatctcaaa cttacatggt tacatgagaa taacaaatcg aaattactca agcttcctat
      301 ggctagagtc gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac
      361 gatcattaga aaggaaatcc tattatgcat agttatcaag aagatgcaaa agtccaaaaa
      421 aatcgctggt ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc
      481 agtattgtca atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa
      541 gcggaatggg tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt
      601 aaaattggcg atagctgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc
      661 ttgggctcgc tgttgtgtgg gttcaatcat tcgctaggta tttaattgtt cgcacgggtg
      721 gtacaagcca ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc
      781 tttcccatac atgaacgtgg tcgggcattg ggagcaatcg gtgcgcttgt ttcattaggc
      841 tcgattgctg gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt
      901 ttttggatca atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca
      961 aaagatatca ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct
     1021 acgattatca gcctttttgg aacaatcttt ttggggcaag aaagtggctt tttccatcca
     1081 gtcccccttg tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa
     1141 aagaaagcag cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg
     1201 agcttgatta cagccttgtt gattttttct tccaacttct ttgttaatgt agtgattcct
     1261 ttttatctgc aaaatgctcg cgggttgagt cctagctatg ccggactgtt gatgatggtc
     1321 tttcccttga tgatggtcgt cggggcgcct ttaagcggat acctgaccga ccgggttggc
     1381 ccggagattt ttgtattagt aggcttgggt ttattgtcgg taacccaagt catgtatatg
     1441 tttttgtcag tggactctcc tttgtggctg tacattgtcg ctactgccat catgggtgcc
     1501 ggcaatgcct tgttccagtc cccaaataat acgatggtta tgagttctgt cactatcgaa
     1561 aatttgggac tggcaggctc catgaattcc tttgcccgaa atttggggat ggtcatcggg
     1621 atttcgcttt ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccaacgagta
     1681 actaactata tcgcagatcg gccggatgtc tttttatatg gaatgaagat taccttttta
     1741 ggcagctttg ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa
     1801 ggaggtattc agaaaaatgt ctgaaaaacc attgttgaag aatttttgga ggcctgcgta
     1861 tgggtgagtg ggatttccat ggatgatgga agatgaagtg aacttctgaa aaccgaaaaa
     1921 ctcaagtatt atcttcaaaa tactagtact caattagatg ctcgatttga ttacaagaag
     1981 actgcgaaag ccactggcaa ccgcaagcta ggtatccacc gatggactac gttttagagg
     2041 caactggtta tgaatacgga gctaacgctc tcatctgcat aagaatgcaa cacccagatt
     2101 atgtaaagat tttcgatact tctgtcaaaa aaatcatcaa gatagataaa tgatttccgt
     2161 ctagtcaaat tggttcagaa tgtggacaca cagatggtaa gaagtcattc acaattcgag
     2221 aatggacttg tcctatttgc cgtacacatc atgatcgaaa cgtcaacgtt agtatcaata
     2281 ttttgatcaa gggctatgta tacaagcatt gccttagaaa acatcaagaa ccttaggaac
     2341 aacggggata gattggttaa taagagatat cgctgttagt aaagacatag gcttgcaagt
     2401 atactctttt atcaagaatt ccgtggcttt agtcatgata ggtttaatac tatgaaaatt
     2461 ttctttagca tttttgattt aggttccttt tctaattata ataaaatgac cactacagta
     2521 acctactgaa atatataaac agccactgta gtaccatttt caaagtagtc tatgagagga
     2581 gaatcttcat tcttatgaac gctggaattg cggatatgaa tttaataaaa aaaaccctta
     2641 atgattttac ttccaattca atatcaaaag gtactggtat caatttaagc acaataaaaa
     2701 agctgaaaag tggtgaaaga tcagtggaaa agctgaatct tttagatgct ataaaaataa
     2761 cggaatttgc tatgaaaaat ggaaaagctg agattgaaat atggagataa ttatcgtcta
     2821 gtgaactact tgccacttag ctgaatttaa cgatccttag tgcttgaagt gggagattct
     2881 tgggaataga gcatacttgc aagcttatgt ctttattaac agcgatatct cttattaacc
     2941 aagctatccc cgtagttcta aggtttttac ttttatctaa gctaatgatc gtattcaaag
     3001 gaaatttttg acccttagaa agtctcattt tatcagacat gagcttaggc attgctgaat
     3061 tttgttaatt cggttttagg ttgtgctttg agcataatat gaaagtagtc tttatcacga
     3121 tttcactcaa ctaaggtaat gtgatgtgat tctgatattc tttcaaagat agttttttgc
     3181 ataagctaac attacatcat caatgagatg tttacaatgt ttcactacta aaataaggtg
     3241 gtaacaaaga agaaataatg aatgattatt tgtatctaat tccatggtat atcaaccatt
     3301 tcttatatag actgattatg acatggatta aaataaaaag gcactatgtc ttaattgtcg
     3361 gttttcgagt taccgaacgg cattcatcac ccacctatag aggatgggcg acttctgcct
     3421 gaaataagtt aaactactta taacttctca taaatttaga ttgagtcatt attatttaac
     3481 atcaatgatt ttccagtttc catttttttg taatattaat ttgtactgaa agtagctagt
     3541 ggtttttgat acattatcaa ggtattttat ctttgtactc actagaattt tttttccgtt
     3601 ttgtaggtaa actggatcct ctaacttcga aaactttaag tcaatattga ttggtcgcat
     3661 cgcatcattt tcaacataat actccaattc tttttcagaa gctgatggat ataatttaaa
     3721 gaacgtttcc aaaaactcag cgatttcttc tgtagttttg gcatcaattt tcgaatcatt
     3781 ttctacttgt tttgtcttgt attttgccct atcaggtttt gcaatcatag ttggcaaact
     3841 cgttactata taattcctgt tttcatcttc aaagatcgtc actcggtatg cagaagaaat
     3901 cgattgtgcc ttttttccct ctgtaatctt ttgctccact aaaaatgaaa cgacaaattc
     3961 ctccgagctc ttttcaacat ctaagacttt tactgactga acttcggaat tagtgggaat
     4021 atctgctcga accatatctt gacttaaagc gaaaccttct tctgtaaggt actgttcgag
     4081 gttgttcatt ctcttttcaa ttgcttcttt gttatttttc cacgaaaaat attctttcga
     4141 aaaatccttc gtaaagtttt caataccaga agtattcact actttctctt taataaccac
     4201 tttttcatga gttgtatgtt gatcaatggc ggtgaaattt ttataaacgc caaaagtaag
     4261 actacaagat aataaaagcc aaagggcaag caccattttt cgatgcttac ccacggatac
     4321 gactcgttct tttttctttt ttaggctctt ttccttttga ttccgttcaa tttttatctt
     4381 cattcttttt cctcttttct cttatttgta ccgtccagca ccgataagat gctgctgcca
     4441 atagctttct gttagattcg tccagcctac tggatctcct gcatgaaaca tgcgattctc
     4501 ccctgcataa ataccgacat gggtcacata agtaccggca tcataagtcg aatgaaagaa
     4561 aaccaaatcc cctggtttgg catcttttag gtcaatatgg gtcatggcat catactgatc
     4621 ttgagccacc cgaggaagtt tcagaccaac tttggcataa caccattgag tcaagccact
     4681 acaatcaaat cctgtttcgg gtcttgaccc accaaataca taagccgttc cttcatactt
     4741 aaacgcctcg tcaaaaatcc cttggactgt tttatcgtca aactgtgttg tagttaggta
     4801 ttgcttcacc aataagcaat aaaacatatt gccatagtta tagcgccatc caccattttc
     4861 cttattcgcg atgggattag ggtaatcgat tttttgaccg caagattttt cctttgaaaa
     4921 agattcagct aaagcaaaac tatgcttctt cccgtttttt gccacatagt ccaaataacc
     4981 accgccaaag ttataggcct gaatgatact atcttgatca caacctaatt ctttggaacg
     5041 cttaaccaaa gacgcaaaat agagacatcc ttgtttgatg gatgcctcgg tacttaatga
     5101 attcggtggt aaaccggctg actcagaact ttgcatcaca tcttccattg tgccaccaga
     5161 ttccacttga ataatcgcta aaagaatcgg aacatactct gttatatccg attctttggc
     5221 atatttttcc accattgcac ggtgtttcaa aacctcttct gagacactac tccccccagg
     5281 aggagcacct gaactactac ttccttcact gtcttcacca aacaataagc ccacacacat
     5341 caagataccc ataaaagaga ttgttaggaa agttaacgaa atggcactta atttctttac
     5401 gttcattttt tctcacctga ccttggtttg ggatgagggt tcaaactttt ttgtactttt
     5461 cgattggttc gtttggacag agtagaccgc cgtttcactt gttgaaaaga atttttctta
     5521 cttgaggatt ggtttattcc ttttgaaatg atttcttggc tagcgaattt tcgtttgatt
     5581 tcgggattcg aatttgctct cacaatccgc tttggtgctc tatccttttg agattggacc
     5641 tttgctttta tcggaacatt ttcccttctt ttcccatgag gattccctat ttcgttcaat
     5701 actttgcgct tttcagctat tttttcgtct ctacgttgtt gttgtttttc tcgcaactct
     5761 cctcgatttg ctttttcttg gaccagtcct cgcttaaact cttccggtgc ttttttcgtt
     5821 ttttcaattc ctttatggaa gttgtatttc aagttggttg gcgtattccg tactccatcc
     5881 tttacttgtt tggtcttatc tttcaccaaa gcttgggtat ccagaacctt gccgactttt
     5941 ctcccggcca aattcattcg ggattgtttc ttattatttg gaattggctt ttccttattc
     6001 aagtcagatg gtatctcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     6061 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccaac tgctgcacca
     6121 ccaccaaaag ccaaagtccg tcccacattt cgattgagtt gccgcaatgt tcgattcatt
     6181 aacatttgcg gttttcgcat gacccgtctt cccattgatt ggctatcatt actctggaga
     6241 ttgaacatat tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     6301 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     6361 ctaaatgcag tcgtaatcac caaagtaact cctgctcgca tcataatcgt attgaacaag
     6421 cgaataatcg ccttcttgcc caaactctca taagtcggca acatggacaa taagaaacta
     6481 atcggcaaaa acatcgcaaa gataataaag agaatttgcg agaacaacat aatccctgtt
     6541 aggagaaaga caaagaagga tatgccaata ttgaacaaca ccaaaaagac cacggtccct
     6601 aagcggttca tggtcttggt aatactgaga ttcgcattgt cattatcttc aatctccgct
     6661 ttgaccgctt cttcccgatc cttgccttta ttttcatctg gacttacaga caggattttg
     6721 ttaacacgat cttcgccaac ttcttctata ttggaatcat caaattgcaa gagtaaccac
     6781 ggttgttgga cttgaatgga aaacaagcta tctcggatta aatccacact gtcctttcct
     6841 tggctttctg aattcggcac gacaatctcg gttccaaggc taagagccgc ttcgctgaca
     6901 tctgagctaa aatcattgat ttttgtgata taggtgggcg catacgcaat gaacgaaccc
     6961 gaaagtagaa aaatcatcac aaaattaatg acagcccgga ctgctttggt agtttctcgt
     7021 tttaataatc ctgtatacgc cacataaact cctagagcta aaatcatcaa taatagaaac
     7081 ccaaagtaaa agcccgaggt ctggagtcca ttttcggtaa tgccggctaa ggtttggata
     7141 ttcttcccaa tactttcagc cgtatctgaa atgaaatcca gcttataggc ttcttgaacc
     7201 acgtagcccg tggcatttga taagtagaga ctaactgtcc aaataaaatt agtaatggca
     7261 taaagtccat acattacgct cttccctagg ccatcacccc aattccaagg cagccaatcc
     7321 cacgaactat ccacaaagaa atccaactga tagttaccaa gtgaatattg ggaataaaga
     7381 tttccggctc caactgtatc atcaaccaga cctgttgcct cagctaccgt tccaactaag
     7441 ctcaaaagta ttatcagagt gaccaaactg attaaaacaa ttccaaagat acgaaaaagt
     7501 ttctttttca tctcctcact ccattctctc ttgaactggc ggacgggtat caaaagcatg
     7561 gaaaagttct tcaaagactg gatgaacttg gaccacacct actcgaccat acaaatcctg
     7621 catcaagcat tgcccatttt ccaaatctcg catgcatttt tgattacttt catcttcttt
     7681 atctaatcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaatgc
     7741 aaactttagc ccaatattat tcttcaaacg ttcatccaac aagtcatcac tgttttgcgt
     7801 gacgaagtaa acaccggcat tcatggcccg accagcacgc actaatttat tcgataacgt
     7861 ttttccttga gctacttgaa gaaaactcca agcctcatcc aggtccacaa tcttgaaaat
     7921 ttctcggttg ctatggataa agtccaaggc aaaagtggaa ataacaatta gcatggaaac
     7981 actcagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
     8041 atccgctact tgaatgatat ttaactgctt atctaaacta atcgactggg taatcgaacc
     8101 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
     8161 attttccgcg actggtgagt cctctttacg caattcaacg attacttgta ataatcctcg
     8221 ctgatcactt tgggtaaccg ttcgaatggc tttgcgtaac acagggaact tctccccatc
     8281 ccgactggaa atccctgtta aaaaggtcag gatatcaatc gccaaacttt ctgagtcttt
     8341 ctttttcttc ataatcacat agggatcaag taagccttga ttttctggct tactggtcaa
     8401 gttaagaata ttaatttcat ccgcaatttc tggcaacgtt tctttccaac cgcctc
//
LOCUS       DAGCXG010000101         8448 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGCXG010000101 DAGCXG010000000
VERSION     DAGCXG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.104"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..360)
                     /locus_tag="MB022_002307"
     CDS             complement(<1..360)
                     /locus_tag="MB022_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467283.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKLCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGI"
     gene            complement(657..3038)
                     /locus_tag="MB022_002308"
     CDS             complement(657..3038)
                     /locus_tag="MB022_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4467284.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3028..3675)
                     /gene="recU"
                     /locus_tag="MB022_002309"
     CDS             complement(3028..3675)
                     /gene="recU"
                     /locus_tag="MB022_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBT4467285.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3739..4278
                     /locus_tag="MB022_002310"
     CDS             3739..4278
                     /locus_tag="MB022_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBT4467286.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4368..4787
                     /gene="gpsB"
                     /locus_tag="MB022_002311"
     CDS             4368..4787
                     /gene="gpsB"
                     /locus_tag="MB022_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBT4467287.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4809..5176
                     /gene="rnpB"
                     /locus_tag="MB022_002312"
     ncRNA           4809..5176
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MB022_002312"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5385..6551
                     /locus_tag="MB022_002313"
     CDS             5385..6551
                     /locus_tag="MB022_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBT4467288.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6597..8093
                     /locus_tag="MB022_002314"
     CDS             6597..8093
                     /locus_tag="MB022_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBT4467289.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8174..>8448
                     /locus_tag="MB022_002315"
     CDS             8174..>8448
                     /locus_tag="MB022_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4467290.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMI"
BASE COUNT         2692 a         1507 c         1768 g         2481 t
ORIGIN      
        1 aataccattt ataactaaac ataaactaat ggatttgggc ggtttcaggc tcatcagaaa
       61 aatatagatt ctggtgaaca acaaccataa aaaagtgaga atcattgcat gagagcttct
      121 ttccataaat tttcctttaa gagtagtgct taaaagtaca gaaaaaataa taaatgcaag
      181 gataggagca attaaagtaa atttatttaa taataaacat aatttttcag cttttttatt
      241 cttggaataa cgaggtattg ttttcgataa aaaaagatgg tgcagacaaa ttatgataat
      301 taagcatact atcagataac tcaaaatgaa gccttctttc aaaaaattat tcaaaaccat
      361 atattgtatt ttttatttga taataacaat gggattatta agaagtggca ctaaataaaa
      421 gactagtgtg tatttagccg atttaattat aaacaagtaa atggataaag tatatgtaat
      481 gataaaagaa aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg
      541 ctggatagtc ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct
      601 gttttttaca tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat
      661 ctgtttccag aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg
      721 ctagttgctg gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct
      781 tcggatgaag aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg
      841 ctttcttttg aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc
      901 gttgtctctg ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt
      961 gatggtgtaa ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc
     1021 acacgaatta atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac
     1081 tgcataagct cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggtgtc
     1141 atttttttat tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat
     1201 aggatatctg gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa
     1261 ttggatgttc cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt
     1321 ccttcagtga tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg
     1381 gctgtttttc catctggctc ataagtttct tctgttccgt cttggaagac gattttatta
     1441 acatattgag gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt
     1501 tttaaggaag aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca
     1561 ttcgattgat ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta
     1621 tctgaaccga cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct
     1681 tggcgtaaag taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc
     1741 ccttcatagt tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca
     1801 aaagctggtc cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc
     1861 actgctaagt tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct
     1921 tttactttcc cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg
     1981 taagagacat actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc
     2041 aaattcgtat aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact
     2101 tcattgatga cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt
     2161 agttcttgca gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag
     2221 attttttcgt tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct
     2281 ggataaacat aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt
     2341 tgcggtaaag aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc
     2401 ccgtacaagc cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc
     2461 gatttttttt gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc
     2521 tgatcctcgg cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt
     2581 ccgccttgca gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg
     2641 tcaaccccga tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat
     2701 gttttcggta attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat
     2761 agttcgccat cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc
     2821 aattcgggcg catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata
     2881 cagaaaaatg agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca
     2941 gaggactttc cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt
     3001 ttctgtcgtc tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga
     3061 taatgttgat aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt
     3121 tttgatctct attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc
     3181 cttttgggcg tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa
     3241 agacgaaaac cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg
     3301 ttcgatttga tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt
     3361 tgcttcaaag tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg
     3421 ggtaaagtag gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat
     3481 ttggataggg gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt
     3541 actttcattg attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt
     3601 tcttttttct tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg
     3661 atagcgcaat accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag
     3721 aaagaaaagg aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt
     3781 ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta
     3841 aaaaaagaac tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat
     3901 ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa
     3961 ctatctcttg gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac
     4021 caagaaaaaa aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa
     4081 ccctaccaat cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg
     4141 ggctgtctgc tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat
     4201 gcacagaatt accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt
     4261 caaaatttta gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg
     4321 aaaaaggtag aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg
     4381 tttatagtcc taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc
     4441 ccgttgaagt tgatgaattc ttagacaata tcatcaaaga ttatgaaact tacagcaaag
     4501 aactccttgc tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta
     4561 aaactcaagg agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag
     4621 tgaccaattt tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa
     4681 agcttgatca acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa
     4741 atgcagacac aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg
     4801 aatttgcggt tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc
     4861 gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt
     4921 tggagtaacg gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg
     4981 ccacagtgac gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg
     5041 agcaacccaa acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca
     5101 gtataactgc agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag
     5161 aacatggctt atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt
     5221 tataagaaac aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt
     5281 attacaaaac aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag
     5341 gactgatgtc ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga
     5401 agttcaacct cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa
     5461 gagatcttgg aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg
     5521 aaacgatcgc tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg
     5581 gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg
     5641 aagattttct tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac
     5701 tatatagcac acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta
     5761 cctattatca ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg
     5821 aagtagcttt gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat
     5881 tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag
     5941 cacttattat gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt
     6001 caggtacgct ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc
     6061 gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat
     6121 cagcagccga agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca
     6181 atggacggat gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga
     6241 ttacctttaa gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg
     6301 tggctaatcc gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata
     6361 aagaaatggg cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg
     6421 acttagcgtt tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg
     6481 gtgcattgcg tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga
     6541 aagacgaata atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga
     6601 aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa
     6661 tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag
     6721 tagacagcta tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag
     6781 ccattcgcta tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg
     6841 ccaaagtaaa agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat
     6901 atacagctgc cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat
     6961 tttctgattt tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt
     7021 actggagaaa agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga
     7081 tgactgtcga aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc
     7141 gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga
     7201 cgaaaaaaca acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta
     7261 gtcgtggtcg tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg
     7321 cacgaatcac gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc
     7381 acgaggctgg tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg
     7441 tttatgaagg tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta
     7501 ttggaagtag tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag
     7561 gaacattcga tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa
     7621 gtttggttcg tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg
     7681 aaatcgaaaa aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga
     7741 atcaaaaata tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac
     7801 aagacgttca ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca
     7861 tgtatgcggc acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag
     7921 cgtcagacga ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg
     7981 catctagaaa accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat
     8041 acctgatcga ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa
     8101 tttacaaaga tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa
     8161 agaagtggaa cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc
     8221 gcatgatcaa acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa
     8281 aattttggag acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca
     8341 tgatacgatt gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt
     8401 attccttcgc tctttgccaa tagtagagga acagatctcg atgatttt
//
LOCUS       DAGCXG010000102         8424 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGCXG010000102 DAGCXG010000000
VERSION     DAGCXG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.105"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..299
                     /locus_tag="MB022_002316"
     CDS             <1..299
                     /locus_tag="MB022_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467291.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(396..830)
                     /locus_tag="MB022_002317"
     CDS             complement(396..830)
                     /locus_tag="MB022_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467292.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1062..1772)
                     /locus_tag="MB022_002318"
     CDS             complement(1062..1772)
                     /locus_tag="MB022_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4467293.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1762..2622)
                     /locus_tag="MB022_002319"
     CDS             complement(1762..2622)
                     /locus_tag="MB022_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBT4467294.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2627..3265)
                     /locus_tag="MB022_002320"
     CDS             complement(2627..3265)
                     /locus_tag="MB022_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBT4467295.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3280..3579)
                     /locus_tag="MB022_002321"
     CDS             complement(3280..3579)
                     /locus_tag="MB022_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4467296.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3622..5106)
                     /locus_tag="MB022_002322"
     CDS             complement(3622..5106)
                     /locus_tag="MB022_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4467297.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5126..5587)
                     /locus_tag="MB022_002323"
     CDS             complement(5126..5587)
                     /locus_tag="MB022_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4467298.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="MB022_002324"
     CDS             complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="MB022_002324"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBT4467299.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6890..7660
                     /locus_tag="MB022_002325"
     CDS             6890..7660
                     /locus_tag="MB022_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4467300.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7933..>8424)
                     /locus_tag="MB022_002326"
     CDS             complement(7933..>8424)
                     /locus_tag="MB022_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBT4467301.1"
                     /translation="FYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAM
                     IYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEK
                     GIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIEN
                     DLK"
BASE COUNT         2562 a         1788 c         1335 g         2739 t
ORIGIN      
        1 tggattatac tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt
       61 tcatgtataa agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc
      121 gtataaagcc agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta
      181 ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata
      241 ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc
      301 tgagcattgg aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact
      361 aagctagaat tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc
      421 ttttttcttc tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat
      481 cctactgatt cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg
      541 actggtccat aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct
      601 tggcgctgat acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca
      661 gtgatccaac gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca
      721 gattgttcaa atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg
      781 ctcttatata attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc
      841 ctttcttata caattctccc tctaataata cagttctttg actatttaat ctatcacata
      901 taactctcct gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat
      961 actttttgat aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat
     1021 caagtgttat cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca
     1081 tactttccat gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata
     1141 ttggggttga tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg
     1201 ttttctactg ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt
     1261 actccaggga cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc
     1321 cctgtatgaa cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga
     1381 accttaccat aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct
     1441 tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa
     1501 gtttgataca aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct
     1561 attatatttc ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg
     1621 acaccgcttg gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc
     1681 caagtcaatt tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc
     1741 ttcattgctg caattttttc ttcacaagac atcatagcca gcctccttca atttagaact
     1801 taagtagtct tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat
     1861 ttcaatcaga aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa
     1921 atcaacacaa ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac
     1981 agggtaagta tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg
     2041 agccggatta ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa
     2101 aggtgaatga atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc
     2161 atccatgatt tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt
     2221 tattccttcg ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta
     2281 tccagctatt tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat
     2341 tagttgtttc gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg
     2401 cgcacttaaa caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc
     2461 agctatcttt tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga
     2521 catctcgata aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat
     2581 atcttttggt aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac
     2641 gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac
     2701 cacgaccagt gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat
     2761 ttaatccacc agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata
     2821 aatctttttc accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta
     2881 cctgccggat accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga
     2941 gttctacttg caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac
     3001 aacaaatgac tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc
     3061 cagcgtctgc acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac
     3121 agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta
     3181 ctacatcacc aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg
     3241 caatttgcaa attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca
     3301 ctttgtttca gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat
     3361 aactgtaact ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa
     3421 aactacatcg tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt
     3481 ggtaattggt acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct
     3541 tgttcctgat ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt
     3601 gttgtctaat cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc
     3661 tacaatcaaa aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa
     3721 accaatgaca actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa
     3781 tggccaagct gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat
     3841 ccatgtagcg aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag
     3901 gcaagcggct ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa
     3961 gaacaacgga atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc
     4021 aatcatcaaa aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga
     4081 accaaagcca aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt
     4141 tgagattcct tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa
     4201 gatcgccaga tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg
     4261 cccttcttgc atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat
     4321 cccaaagaaa aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa
     4381 gaatcctggt aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt
     4441 ttcatcccgc tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg
     4501 ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa
     4561 gttagaagct actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat
     4621 cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac
     4681 ctgaacgttt ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa
     4741 taagatattt aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc
     4801 aaatgtctcc attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat
     4861 ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa
     4921 accaccagag ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat
     4981 gcattcatga agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag
     5041 gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc
     5101 ccccatcttt tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat
     5161 cgatggagtt cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata
     5221 atttttggat attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac
     5281 tagccgtttt ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg
     5341 taaatgaaat agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta
     5401 cttgaggtgc gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca
     5461 catacgtttc atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt
     5521 cacgccaatc cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt
     5581 tcagcaagaa actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat
     5641 cgagaagaca tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg
     5701 atagatgaat ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc
     5761 ctttttgaat ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat
     5821 atcgtagtgg acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc
     5881 aacagaagta actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac
     5941 atcgattttg tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc
     6001 atgataaaga ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc
     6061 ctgaggcatt tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg
     6121 atcaaacgct tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt
     6181 tactgtaatc gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt
     6241 tggcccaatg aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc
     6301 tagatgatcg gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg
     6361 atcgattacg aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg
     6421 catcatctga tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca
     6481 ccatagatca cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc
     6541 cagccaccac atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa
     6601 gtatgtgccc cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac
     6661 agttttactc atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa
     6721 tgtgtatcag catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct
     6781 ttcagtaatc aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa
     6841 ctagtcaaat aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc
     6901 acttgtcaat attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca
     6961 actatcaact ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga
     7021 tttaaattta ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa
     7081 ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc
     7141 cagagaagct agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac
     7201 tgcgttgaaa acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt
     7261 gaaaatcaat tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat
     7321 gcgctttcca aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa
     7381 tgcagacatt tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa
     7441 gatattaaat gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat
     7501 tttagtcgct gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc
     7561 agtctttgat tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag
     7621 aaatacgggg ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt
     7681 aaataggatt atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc
     7741 tcaaattgat aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg
     7801 cttcttttat aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag
     7861 gtatgacaaa agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat
     7921 ttcacaacca ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt
     7981 gaaacgcgct attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg
     8041 cttatggcaa gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt
     8101 cttattatac tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt
     8161 acggtaatag gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt
     8221 ctcactcaat attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt
     8281 tgacaagtag atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt
     8341 cgtttgttcc aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag
     8401 atgatcaagc agtccttgat aaaa
//
LOCUS       DAGCXG010000103         8073 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGCXG010000103 DAGCXG010000000
VERSION     DAGCXG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.106"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(17..538)
                     /locus_tag="MB022_002327"
     CDS             complement(17..538)
                     /locus_tag="MB022_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4467302.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLLRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTELKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIKELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="MB022_002328"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="MB022_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBT4467303.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELQKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVIPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYVQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPQWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYLGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSTMPNFMRKQVRFSSLENANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="MB022_002329"
     CDS             4618..5505
                     /locus_tag="MB022_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBT4467304.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="MB022_002330"
     CDS             5502..6671
                     /locus_tag="MB022_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467305.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVKADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="MB022_002331"
     CDS             6695..8050
                     /locus_tag="MB022_002331"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBT4467306.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEVEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2602 a         1487 c         1822 g         2162 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattccttt attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt tagttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttgaccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgcagc aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaacagatat atgtaaaata tttttataaa
      661 taaaagaaaa aaatggaatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggccgaatta gtagatcaat ggtcagcagc aggaaaagaa aatgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttctcaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gctttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtacc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcattatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgcaaa aagacccaag catacgcaaa aaacgtttca ccatagggat
     1921 cgttgatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttgggggtt tggatctgac ggaacagtcg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctaactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttctgcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aataccgcaa tggaaacggc attttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg attccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatgta caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tcagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttggt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattgtcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ttggaccttc tctaatcatt gcctatacgc cttgtatcac
     3961 acatggtttg cttggtggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggctattgg tcattatatc gatataaccc tgcaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttttc tactatgcct aatttcatga ggaaacaagt
     4141 tcgtttttcc tcgctagaga acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcaga tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgtt gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagcgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 ctggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg tatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg aaagctgatt
     6181 caatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacgca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaatta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacatcgt cgacggtgat aaaattatgt ttatctgtgc
     7501 taaatatttg gcagaaaaaa atcggttgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggtagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gtcaattact atgtcgataa aatcgcagca gtagtcaaag aagaaattgg
     8041 actggattaa gagatatttt cataaaaaat aaa
//
LOCUS       DAGCXG010000104         7466 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGCXG010000104 DAGCXG010000000
VERSION     DAGCXG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.109"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(57..350)
                     /locus_tag="MB022_002332"
     CDS             complement(57..350)
                     /locus_tag="MB022_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBT4467307.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="MB022_002333"
     CDS             complement(378..1043)
                     /locus_tag="MB022_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBT4467308.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="MB022_002334"
     CDS             complement(1183..1815)
                     /locus_tag="MB022_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4467309.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="MB022_002335"
     CDS             complement(1822..2889)
                     /locus_tag="MB022_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4467310.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="MB022_002336"
     CDS             complement(2886..3617)
                     /locus_tag="MB022_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBT4467311.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="MB022_002337"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="MB022_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBT4467312.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="MB022_002338"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="MB022_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBT4467313.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="MB022_002339"
     CDS             5780..6949
                     /locus_tag="MB022_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4467314.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         2260 a         1506 c         1143 g         2557 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     7381 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     7441 tacaggtaaa actattgttt tgcctt
//
LOCUS       DAGCXG010000105         7333 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGCXG010000105 DAGCXG010000000
VERSION     DAGCXG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.110"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..666
                     /locus_tag="MB022_002340"
     CDS             <1..666
                     /locus_tag="MB022_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4467315.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
     gene            678..1598
                     /locus_tag="MB022_002341"
     CDS             678..1598
                     /locus_tag="MB022_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4467316.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1639..3096
                     /locus_tag="MB022_002342"
     CDS             1639..3096
                     /locus_tag="MB022_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4467317.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3441..4055
                     /locus_tag="MB022_002343"
     CDS             3441..4055
                     /locus_tag="MB022_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4467318.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4048..5772
                     /locus_tag="MB022_002344"
     CDS             4048..5772
                     /locus_tag="MB022_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4467319.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5769..7220
                     /locus_tag="MB022_002345"
     CDS             5769..7220
                     /locus_tag="MB022_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4467320.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2503 a         1221 c         1466 g         2143 t
ORIGIN      
        1 atccttttga atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat
       61 aaacgactcg ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc
      121 attatcagtt actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg
      181 atcactggac atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt
      241 atcttggtgt tcattggtac atggaaaggg attggttata acagcattat ttactttgca
      301 tctgttatgg gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa
      361 tggcagcaga tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg
      421 attttagccg taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg
      481 aattccggtg cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg
      541 acatccacag gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc
      601 tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta
      661 ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca
      721 ataagacggc taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt
      781 tgccatttat atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg
      841 ggtacagttt ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga
      901 tgcaggataa aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac
      961 tgatcaatag cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat
     1021 tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg
     1081 ccaattatct tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct
     1141 tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg
     1201 tctctgattc gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg
     1261 ttcagatcgt tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat
     1321 tgggttactg gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc
     1381 cactacagta tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag
     1441 gagcaggaag ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg
     1501 cgatcgtagt catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt
     1561 ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata
     1621 aaattaggag gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg
     1681 ttgttgttgg gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct
     1741 agcaattcgg acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa
     1801 ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat
     1861 tacattggct ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat
     1921 tatgatatcg cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat
     1981 ttgactgaat tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat
     2041 attaaaggaa atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt
     2101 gctcaacaag tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat
     2161 ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca
     2221 aacacggcag cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct
     2281 ttagggaata cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac
     2341 caatacgata atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa
     2401 ggactcattc caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact
     2461 tggctaatgc gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat
     2521 gcagcaaaac aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa
     2581 gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa
     2641 cttctagggc tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag
     2701 ggagaagcat gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat
     2761 cagccaaata cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa
     2821 gagtctatta cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact
     2881 tcaccgattt taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt
     2941 tctaatgtca tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa
     3001 acattaccga aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa
     3061 atgcaaaaac aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg
     3121 tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct
     3181 cggtattaag ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac
     3241 tactcgaaac taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctgacc
     3301 ctcggtattc gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta
     3361 ataatcaggt caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact
     3421 taatgtttgg aggagaagat atgttaggga aggttttgga agagttattt atccggatct
     3481 gggtcattat aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct
     3541 ttggattagg tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt
     3601 ataaagaaat cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca
     3661 atctgctatt tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat
     3721 ctgttcaaat caaagggctc atttttttag ctattgattt tattttactt tttgccttgc
     3781 tctgcctatt tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg
     3841 ttcctaatct gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa
     3901 aaataattat tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc
     3961 tgtttggtgt cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg
     4021 aagaattaga taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga
     4081 accttactaa atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact
     4141 gctaccgtta tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg
     4201 acaagtgatg ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg
     4261 atcatcaaaa accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta
     4321 acaaaaccca tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa
     4381 ttagtttctt tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg
     4441 atatatatcg tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt
     4501 ggggaaataa aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc
     4561 acacaaggag gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat
     4621 atgattttag aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg
     4681 acaaatcgac tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa
     4741 gaaatgatca tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa
     4801 aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta
     4861 gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc
     4921 attattttag acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag
     4981 caagtatcta tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg
     5041 atcgacataa caaacacgga atatgaactg aaggaacttt caatcggtat caacgaaatg
     5101 ttggatagca tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac
     5161 gcacatatgc gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa
     5221 tatatccgaa tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg
     5281 ttttccgctt tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa
     5341 ctggactttt gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc
     5401 tatcatttta tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag
     5461 ccattagttg aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta
     5521 agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa
     5581 gggatgactg aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa
     5641 cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc
     5701 agttattata tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc
     5761 aaggagggat gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg
     5821 ggttaaaaaa gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga
     5881 atgccaaaga aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg
     5941 tgacaatgcc agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata
     6001 attttgaatt tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc
     6061 aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc
     6121 tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct
     6181 atcaagaact gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag
     6241 aaataatagg acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa
     6301 ctcatgatac tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac
     6361 gaaattacgg agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct
     6421 tctgttgttt tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag
     6481 agacagattc tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt
     6541 tgcatcaatt ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa
     6601 aagatcaaac catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag
     6661 tcgctcaaaa aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg
     6721 aagatatacg atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc
     6781 tggaagatga ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag
     6841 aacttcagca gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg
     6901 acaatgtgga aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca
     6961 aagaagtgtc agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag
     7021 aaacgaaaaa aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac
     7081 tgcttttgca cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca
     7141 ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag
     7201 aaaacagtgg agaatcataa aaaaaaaagc caatgtaatg aaataactta taaaaaattg
     7261 tctagaaatc taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc
     7321 tctcgataaa cgg
//
LOCUS       DAGCXG010000106         6959 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.111, whole genome shotgun sequence.
ACCESSION   DAGCXG010000106 DAGCXG010000000
VERSION     DAGCXG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.111"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            131..988
                     /locus_tag="MB022_002346"
     CDS             131..988
                     /locus_tag="MB022_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4467321.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            981..1883
                     /locus_tag="MB022_002347"
     CDS             981..1883
                     /locus_tag="MB022_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4467322.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1916..2266
                     /locus_tag="MB022_002348"
     CDS             1916..2266
                     /locus_tag="MB022_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4467323.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2315..3238
                     /locus_tag="MB022_002349"
     CDS             2315..3238
                     /locus_tag="MB022_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4467324.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3316..4209
                     /locus_tag="MB022_002350"
     CDS             3316..4209
                     /locus_tag="MB022_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4467325.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4247..4810
                     /gene="amaP"
                     /locus_tag="MB022_002351"
     CDS             4247..4810
                     /gene="amaP"
                     /locus_tag="MB022_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4467326.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4824..5012
                     /locus_tag="MB022_002352"
     CDS             4824..5012
                     /locus_tag="MB022_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4467327.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5025..5927
                     /locus_tag="MB022_002353"
     CDS             5025..5927
                     /locus_tag="MB022_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4467328.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5959..6201
                     /locus_tag="MB022_002354"
     CDS             5959..6201
                     /locus_tag="MB022_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4467329.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6457..6828)
                     /locus_tag="MB022_002355"
     CDS             complement(6457..6828)
                     /locus_tag="MB022_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467330.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2535 a         1180 c         1384 g         1860 t
ORIGIN      
        1 atgcgttttg tgataaaaaa attttaaaat attcaatgaa aacaaagcat atattttgct
       61 ttaaaaccaa atgaggaata ttctagatgt agaacaggtt agaaaaagat tagaaaaagg
      121 agtgattttg atggagaaga gatacggcgt agttcctgtg gtaactgctt cagacgaaaa
      181 ttatgctcct tacttgagcg tgatgatcgc aactgcatta gaaaattgca ataaaacaag
      241 acgcataaaa ttttatgtga ttgacgatgg tctatcagaa tatagtaaag aaggattgga
      301 agaaacggta aataaatatt caagtaatgc aagtatccaa ttcctgacag tggaaaaaga
      361 tatctacgaa gacttcctag tcagtgacca tatcacaact accgcttact taagaatttc
      421 tttaccaaac ttattagcta aagaggatta taagaaagtc ctttacttag attcagatgt
      481 gttagtacta gacgatatcg taaaactata tgatgagccg ttaaatggaa aaacaatcgg
      541 cgctattatc gatccaggcc aagtgaaagc tttggaaaga ttaggaatcg attccgatga
      601 cttgtacttc aattcaggag tgatggtgat tgacattgat caatggaata aaaaagaaat
      661 tacagaaaaa actatccatt acctcagtga aaatggcgat cgaatcatct atcacgacca
      721 agatgctttg aatgctgtac tttatgaaga ttgggaacaa cttcatccaa aatggaacat
      781 gcagacttcg ctgatttttg aacgtcatcc agcaccaaat gaaaaatatg aacgtcagta
      841 taaagaagga aatgaaaaac cttcgatcgt tcactttaca ggacatgata aaccttggaa
      901 tacgttgaaa gatcatccgt ataccaattt atacttaaaa aaattagcac acagtactct
      961 aacgaaagta ggcgagatca atgaataaaa aagaaatagc agtagtcgca agttgtaaca
     1021 caaaattcgt accacattta gctgcattat ttgtctcagt attagataat tgcaacccgt
     1081 ctaaatttgt tcggttctat gtgattgacg atgatatcga ttttgaaagt aaacagcttt
     1141 tacgcttctc agtgaaaaac gcacgaatga actcagatgt ggaattcttg aaaatcaata
     1201 aagaattctt tacaaatgtg gtaatcagcg atcgtattcc agaaacagct tattatcgta
     1261 ttgcgattcc ggaattgttc cgcggcacag aagttgaacg tatcttatat atggattgcg
     1321 acatgatcgc tttacaagat atctctaaat tatggcgtct tgacttcggt gattcaatcg
     1381 tagcagctgt tgaagacgct gggttccatc aacgactgga aaaaatgaaa atttcagcta
     1441 aatcaatgcg ttatttcaac tcaggtttga tgctgatcaa tgtgaaaaaa tggttagatg
     1501 aaaatattac gcaaaaagta ttagatttca tcgaacataa tccagaaaaa ttacgattcc
     1561 atgaccaaga tgcattaaat gctatcttac atgaccgttg gttaccactt catccgcgtt
     1621 ggaatgcaca aggctatatc atggcaaaag caaaaaaaca tccaacagca gcaggtgaac
     1681 gtgaatacga agaaactcgc aacaatcctt acattatcca tttttcagga catgtgaaac
     1741 cgtggagcaa ggattttgaa gggccaacga agaaatatta cgaaaaatat gcagggatga
     1801 cggctttccg ttgtgtagct aaattcccta aatatccaaa atatgcaaaa gttcaaccaa
     1861 aaacagatgt agttgttaaa taatagaaga caacaactga agagggggag taggaatgga
     1921 aattacaatg tctaaaaatg cagtagagac gttaattgaa aaggttgggg aaaatacaaa
     1981 aatcgcactc gcgcttatca atgattccga tccatttcta agggacaaag gtgcttttgc
     2041 aaaaggcagc ttttttcaaa tcattccttt tgtttcggaa tttggagaat atgcgactaa
     2101 aatcgagcat ccattattgg acatctatac atctaaatta gagcaaaatt atttcggtaa
     2161 acgattgaac atggacttca acaaacagct tgacagcttt tcattagaaa atgaaatcgc
     2221 agtactcgat tataatatca aattaaagaa ctgcttcttc agctaatagt ttcgagaaag
     2281 tccaaaaagg aggtatacat tgagatacta ctttgtggat gaagaaaaga atgtaataga
     2341 aacagatgaa aaaaaatata attggctcgt acttgatagt gcaaagcccg aagagattga
     2401 gcaagcaatc gaagaattcg agcttcctga tgatattttc gtaggaaccg aatatccgga
     2461 agaagtttct cggatggagc gcctttatga gacgaaacta gagcatccat tttctttggt
     2521 agtgattaat ctagatcagt cagataatcg tatcgaaaaa aaattaacgc caatatcttt
     2581 tgtactttcc gataatctct tgattacttg tatcgataga aaaaccgaat ttgtggatcg
     2641 gctgctgaca aagcatggaa atcatttaaa ctcgtttgaa aagatcatca cgtatacatt
     2701 attagatatt tacacccatt acgtgaaaga actaagagag atgaaaaagc gaattgacgc
     2761 tttggataaa gcagctcgta aaacaacgga aaatgaagaa ctgtataaac aggcagatct
     2821 tgaaagagac atcgtatata tcgatcatac cttaagagat caacgggaaa caatggatca
     2881 attatggaat tctgaaactt tcatcaaacg tttagacgat gaacagctac tctatgatgt
     2941 ccatttgaga cagcgacaaa cagaaaaaat gatcgagatc tatcgagatt tattagagag
     3001 tattggtgat ctattcaacg ggatgatgga caataatcta aatcatatca tgaaatatct
     3061 agattcagct gcattaatca tttccgttcc ggcgttgttc gcagggattt ggggaatgaa
     3121 tacaggagga ttaccgggta aaagttctgc tctaggcttt atactcgtag tctttggctc
     3181 tttgatagca gcagtagttt taggttacca tttataccgg aaagattata caaattaaag
     3241 aatctattta aaacaggtat caaacaaaga aatttgtttt ggcaatacaa tttaaaaact
     3301 aggaggaata cagcaatgac agaaccaaaa ttaatcgacc cgcgaaactt gtaccatacg
     3361 gagaaatttc ctaaacaaga tcaggataca ccagctttac agcagaatat gattcctgtt
     3421 cctgattgtg gagaagaatc ctatgaaggc aataatcaac tagaaaaccg ccgtgtacta
     3481 atcactggtg gagattcagg gatcggtcgt gcagcagcga ttgcatttgc tagagaaggg
     3541 gcagatatcg ctcttcaatt cttcccagga gaagaaaaag atgcagagga agtagcaaaa
     3601 tacgtgaaag ttgcaggacg taagattgtc ttactacctt ttgatcttcg tgacgaacaa
     3661 gcacctaaag aaatcataga taaagccgtg agtgaacttg gcgggctaga tacgatggta
     3721 ctaaacgcag gacagcaaat ttcttgtcag tctattgctg agttaccact tgagcaagtc
     3781 aaagacacat ttatgataaa catcatcgct atgtttgctt tagtgaaaga agctgttccg
     3841 catatcccag caggaggagc aatcgtaaca acaacttctg ttcaagcatt caatccaagc
     3901 gaacatttat tagattacgc agcaacaaaa gcttcaatcg ctaactttac gatcggttta
     3961 gcaaaacagc ttgcacctaa aggaatccgt gtaaatggtg tagcacctgg tccaatctgg
     4021 acaccattac aactagacga aggacaacct tctgattcga tccctgaatt tggtcagcaa
     4081 agtttactag aacgtgcagg tcagccagga gaattagcac cagtttatgt tttcttagca
     4141 tcgaataaag caagttatgt tacagcccaa atttacggag taactggtgg agaagcgatc
     4201 aacttataag aagttgatcg tttcacccct atgaggaggt gcgaaaatga ataaaggtgt
     4261 aaaaacgatc ggagtgatcg gaacattact tttgctatct gtgcttttgt ttgcgatact
     4321 attaagtgca acctatccat tgccttttca gctagagaag tttcgattct tttccatcac
     4381 taattcatac gtgcaacaat atgtattttg gcttgccgtt gcatttgcta ttttgacaat
     4441 tatcacgtta cttgttttcc tgttttatcc aaaaacaatc ggaaactttc tactgaaaca
     4501 tggtgatggg gagttgacgt tggataaaaa agcaattgaa ggaatggttc gttctcactt
     4561 gcatgaagat gaattcatcc attcaccaaa agtaaatgtc aaagcaacga aaaacaagat
     4621 cgccgtcaat gtaaaagggg agttgaaacg aacttcagca ctcattggta aaacaggaac
     4681 attgatgaaa gaaatcgaaa atgaagtaca acacatattg ggaacaaaag agccagtgaa
     4741 agtatcggtg aaatacacag gctttgaaga accagctaaa cgaagcaatg accgcaaacg
     4801 agtagaatag ggggggacca aaaatgaaag aactattttc gacatacaag ctccctatca
     4861 tctttggggt acttggattg atattagccg ttttatggat caccgtgggc tttttcaaga
     4921 ccttattgat cttgatcatg gcagcgatcg gcgtcgctat cggtttttat ctgaaacaaa
     4981 cgaaaatctt agatcactat tttaaatctt aggaggaaac atccatgaat aacaataaca
     5041 aaaataataa caataaaatg aacaaccaaa aaccagtgtc tgcacatcca gacccaacga
     5101 caccaaacca atcaaatggt gtgaacgatg aaaagaacaa aacagcacca gtatctgcac
     5161 atccagatcc agtagtccct aacgttcaac aaggagtaga agagaagaaa catgatgcag
     5221 aaaagcatgc acctcatcat ccaggaactc cagaatctaa agttccagaa caacatgatg
     5281 caatcgatcc ttctaaacct gtggaagagc aaaaacatcc ttctgaccat gcgaaagcac
     5341 aagaaaaact tagttctgac cacacaaacg gacaaggaaa acttggttct gatcatacaa
     5401 ataaccaagg aaagcatgat tctgaacagg aagattcaat gcgcggtgag ctgaattatg
     5461 aagataaagt tgtgcaaaaa atcatcggaa tcgcaattga acaagtagac ggtttattat
     5521 ctgcaaacgg tggtttcttc tctaatgtgg caggaaaact tgtgaataca gataatgtaa
     5581 cggctgggat cgaaacagaa gtcggtaaaa aacaagtagc agttgatatg gatgtcattg
     5641 tggaatatgg caaagatatc gaaaaaatct ttgaagaaat gcaagaagta atcggcaaag
     5701 aagtaaaaaa tatgacacat ttagaagtga tcgaagtcaa cgctaacgta gtggacatca
     5761 aaacaaaaga agaatttgaa aaagatcaag aaacggtgca agacaaagta actgacgcag
     5821 cgaaaaaaac cggtgaattt gcttcgaatc aaacagataa agtaaaatca gcagctggaa
     5881 gcggcgcaca aaaagtaaaa gaaaatacag aaccacgggt tcagtaataa ggaagtaaaa
     5941 cattaggagg aacgaaacat gggatttatt tggtcactta tcgtcggagg aattatcgga
     6001 gcaattgcag gagcaatcac gaataaaggt tcttcgatgg ggattattgc aaatgtcgta
     6061 gcaggtttag ttggatcagc acttggtcaa gcattattag gttcttgggg accaagtatt
     6121 gcaggaatgg caatcgtgcc atcattgatt ggtgccatca ttttagtact aatcgtatca
     6181 ttctttgctc gaaaactgta aaaagacttt taaaaagagg tcgtgaaaaa gctgttcttc
     6241 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttgggca
     6301 tattatcgaa gatgcaagct tttgaacact gtttatacgg aataagaggc tgtgacaaga
     6361 cttttgtcac agcctcttat ttatgcgggc aaattcccaa ttagattttt aaaagttttt
     6421 ccttaaatgt gtctttattg atacagagaa gcttctttaa taaaacaatg cctccctaaa
     6481 ttttcttgag ataggaaagg tgaacataca aaataatacg accactttga aaacccagat
     6541 gatccacaaa tggatcgtat gatactgatg aagtaaaaag caatggatca gaaactcaaa
     6601 gagattgaaa aagacaagga agaaataata tctctttttt actcttgtct gttttagcca
     6661 ataatagctc gtaaagtaat ctagacaaac aaatagaagt aaacaaagtt tcgtaagagc
     6721 agcaatcgta tcatgttgct ctgctagacg attgacgagc aggaaaaatg ttggtaatgg
     6781 aatatagatc acgagtaatg aaagaaaaaa gtaaacaata aatggcatac tattcctcct
     6841 aattgtgtga gttcgccctc attttaaaat aacgatattt gtttgtgaag ctttttcgtg
     6901 tcgtaacaaa tgttatctaa tgtattttta aataaataat cattattatc aaaataatg
//
LOCUS       DAGCXG010000107         6949 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.112, whole genome shotgun sequence.
ACCESSION   DAGCXG010000107 DAGCXG010000000
VERSION     DAGCXG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.112"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            127..600
                     /locus_tag="MB022_002356"
     CDS             127..600
                     /locus_tag="MB022_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBT4467331.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            597..2729
                     /gene="feoB"
                     /locus_tag="MB022_002357"
     CDS             597..2729
                     /gene="feoB"
                     /locus_tag="MB022_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBT4467332.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2731..2865
                     /locus_tag="MB022_002358"
     CDS             2731..2865
                     /locus_tag="MB022_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBT4467333.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2959..3603
                     /locus_tag="MB022_002359"
     CDS             2959..3603
                     /locus_tag="MB022_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4467334.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3790..4983
                     /locus_tag="MB022_002360"
     CDS             3790..4983
                     /locus_tag="MB022_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4467335.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4976..6703
                     /locus_tag="MB022_002361"
     CDS             4976..6703
                     /locus_tag="MB022_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBT4467336.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2237 a         1226 c         1557 g         1929 t
ORIGIN      
        1 cattctcaat taccgagagt attctatgtg agaatgattc tcatttattt gcgtaacacg
       61 taagaaaatt agaacatata atgaatgatt ttaatacatg atttatttca caagagagga
      121 atcaatatgc agaccttagc gcaggcaaag cagaatttcg tttatcaagt cgaacgaatc
      181 gaaacagaag aaaaaacaaa acgccatttg caaaatatcg ggatcgttgc cggaagtaaa
      241 atcgtcgtcg tcaatttatc aggcaacaat gccattttac tggtaaaaag tcatcgagtg
      301 gcagtaagtc gggaaatact aggtcaaatc tttgtcaata aaaagactgc ttcctctgaa
      361 agctggactt ctcttgatca attaaaagca ggagagactg ggatcgtcgt agcaatccac
      421 ggacaaggcg cagtgaagcg acggttaatg gatatgggat tgacgcgaaa tgtaaaaata
      481 gttatccgaa aactagcccc tctgggagat ccaatcgaac tgacagtgcg agggtatgaa
      541 ctgacgttac gaaagtcaga ggccgaacta atcttgatac aaagggagga aaaagaatga
      601 atatcgcgtt agtcggaaat ccaaacagcg ggaaaactag cttgttcaat ctcttgactg
      661 gttcgaatca atatgtaggg aattggccgg gcgtaacagt agaaaaaaaa gaaggatcgt
      721 tgaaaaaaaa tccgaacatt catattcagg acctacccgg catctattct ttgtctcctt
      781 atacaccaga agaagtgatt gcaagagatt atttggttca ggaacagcct gaactgatca
      841 tcaacattat tgatgctacc aatttagaac gaaatcttta tttaactacg cagttattgg
      901 atttagacat tcctatgatc attggtctga atatgatgga tgtcatcaaa aaagaaggaa
      961 aaaaaatcaa tctagaaaag ctttcctatg gtcttggtgt accagtttta ccaatcagcg
     1021 tattgaaaaa gcaaggaatc gaaaaactaa tcgaaaagat tcaggagtct gcaaaaaagc
     1081 ggccttctat tcctgttccc tgtacatatg atccacgttt agaagcagct ttacatgaaa
     1141 ttgaagatgt actaggagaa caaagcggca agaaacgctg gtatgcaatg aaatattttg
     1201 aacgagatca gaaggtaaga gaggattacg agatagcagt ttctcagcaa aaagagatcg
     1261 agcaactgat ccagttaaca gagaaactat tggacgacga tagtgagacg attctggtca
     1321 atgaacgtta tgaatttatt acccaactat gtacactctg tgttgtgtca aatgattctt
     1381 tccaattatc gatgagtgac aaaatcgatc agattgcaac gaatcgttgg ttagccttgc
     1441 cgatcttcgc atttgtgatg tggctgatct attatctggc tattcaaaca gtaggaacaa
     1501 tggggacaga ttggatcaat gacacattgt ttggtacatg gttaccagaa catgttggca
     1561 gattgttagc tgaatggcaa gtagcgggat ggatgcagga gctgatcttg aacggcatca
     1621 tcgcaggcgt gggagcagtg ttaggatttc taccgcaatt gattatattg tttttatgct
     1681 tgagctttct ggaagactgc ggatatatgg cgaggatcgc ttttgtcatg gatcgacttt
     1741 tcaggaaatt cggtctttca ggaaaatcct tcattcctat gctgattgct acaggatgtg
     1801 gagtgccagg cgtcatggcc agtcgcacga ttgaaaacga gcaagatcgt cgtttgacga
     1861 tcatggtcac aaccttcatg ccttgttctg caaagttgcc gattattgcc ttagtagcag
     1921 gggccttttt tcctcatcaa tcttgggtag ctccttctgc ctattttctt ggtatgactg
     1981 caattatttt ctctggaatc agcttgaaaa aaacacgttt gtttgctgga gatacagctc
     2041 cttttatcat ggaattgccc gcatatcatt ttcctcaatg gtcagcggtc ctaagacaaa
     2101 cctatgaccg cagcaagtct tttgttaaaa aagcaggaac gattatcttt gtttctagta
     2161 tcgtgatctg gttcagctct tcttataatt tttatgctca acctgtttct gaagaccaaa
     2221 gtatcttagc ctttttcggg cgaatcctgg cagttgtttt tcagccgctt ggttggggaa
     2281 actggcaagg aacagtcgca gcgattacag gattggtcgc taaagaaaat attattggca
     2341 catttggtat tttgtttggt catgcggaag tatccgaaaa tggcagagaa atctggcaag
     2401 tgctacaaca cacatatact cccgcagcag cttattcatt gttagccttc aatttacttt
     2461 gtgcgccttg ttttgcggca attggggcta tccatcggga aatgaatgcc aagaaatgga
     2521 cttggatcgc cataggctat caatgcggtt tagcttattt agttagtttt gtgatttatc
     2581 agttgggaca cttattggag ggtggacagg tagcgttagg gacgtatctt gctattttac
     2641 tgatgatagg actcgtttat gttttgttcc gtcagccaaa atccaagaac cagttttata
     2701 ctatattgtc attagaagga gaggaatagg atggcgacat tcttattaag tgtgttaatc
     2761 tttggaagtg caggcgcaat tgtctatact cgtttaaaat ccggtaagaa ttgtgatgac
     2821 tgtcaaacag cttgcccagt aaaaaaggaa cagatcaagc gatgatcagt cgttgcaaga
     2881 ggctattctc cccttttttg cttattttgc tagagcatag taagatgagg gaaagtaaaa
     2941 aaagaaagag gaaagaaaat gtctggaaag aaaaaaatcg tccgcccgcg ctaccagcag
     3001 attgctgttg atttagccga acgtatcgtt gaaaaccgat ataaagtggg tgagaagatc
     3061 catgcccgct caacacttgc cagtacgtac aatgcttctc ctgagactac ccgcaaggct
     3121 atcaatgtgc tggtagatct tggaatcatg gaagtacggc atggaagtgg ggcatttgtt
     3181 gcttcgaagg aaaaagcaaa agatttccta ttgcaatatc aagatgtaca atccatccaa
     3241 gatacaaaag cagaaatcat ggcaagtgtg gagaagcaac aagatgagct gaatcatttt
     3301 tctcaattat tggatcagct ggtcaatcaa acgaagcgaa tacataagat gaatccttta
     3361 cttccgtttg aattatcttt atcagaaaaa gcaatgcatc tggaccgttc aattagcgag
     3421 ttgaatatct ggcaggcgac tggcgccaca gttatcgcaa tttctcataa tgaagaactg
     3481 cttgtttcac ctggccccta tgctaaattt acggcagggg acatcgttca ttttattgga
     3541 aatgaatata cactacagcg tatgacaaac tttttttacc cggagcatca gcttgcagaa
     3601 tgattgtgtg aaatattaaa aaaatattaa cttggctaaa ccttttattt acaaagattt
     3661 agccaagttt ttttcagttt ggacaagcaa atttctttca tttttgacaa agtgactaca
     3721 tgatgttaac atagtgtggt cacttttttc agaaagaaaa atgaatttac ataaaaaagg
     3781 agaatttgtt tggtaaaagt agaagtaaaa catttaacaa agatatttgg taaaaagaca
     3841 caagccgctt tagatatgat gaatgatcac cagccgaaaa cggagatttt aaagaaaacc
     3901 ggtgcaacgg ttggtgtgta tgacgtgaat tttgacgtaa aagaaggaga aatcttcgtg
     3961 atcatggggc tttccgggag tgggaaatcc acattgatcc gtctattgaa tcgtctgatt
     4021 gagccaactt cgggcagtat ctatattgat ggagaagatg tatcgaagtt aagcaaagaa
     4081 gaattgcgag aagtccgtcg tcataaaatc aatatggttt tccaaaactt cggtctgttt
     4141 ccacatcgaa cgatcttgga aaatacagaa tacgggttag aagtccgtgg tgtacctaaa
     4201 gaagaacgac aagaaaaagc agaaaaagca ttagaaaatt ctagtttgct ttcatttaaa
     4261 gaccaatatc ctagtcagtt atctggtggg atgcaacaac gggttggcct agccagagct
     4321 ttagcaaatg atcctgagat cttgctgatg gacgaagcgt tctcggctct tgatccgctg
     4381 atccggcgag aaatgcaaga cgaattactg gacctgcaag ccaatgttca aaaaacgatt
     4441 attttcatta cccatgattt gaatgaagct ttacggattg gggacagaat cgccttgatg
     4501 aaagacggcg aaatcatgca gatcggaaca ggtgaagaaa tattgaccaa tccagcaaat
     4561 gattatgtac gtgaatttgt ggaagaagtc gatcgttcca aagtgcttac ggctcaaaac
     4621 atcatggtac ctgcattgac gacgaatatc gaatctgatg gaccaaacgt tgcattgaca
     4681 aggatgcgta atgaagaagt cagcatgctg atggctgttg acagaaagag acacctgaaa
     4741 gggatcatca ctgcagatca agcgttagaa gctaggaagc aaaaacgtcc attgatcgac
     4801 ttcctagatg aaaatgtgac ggtgattggg aaagacatgg tggtaagtga tattttcaat
     4861 attatttatg actcaccgac tccacttgca gttgtagagg atggaaaatt aaaaggtgtc
     4921 gtcatccgag gtagtgtgat tgaagcatta gcagaaacaa gcgaggtgag tgaacatgaa
     4981 taatctattg aactatcaat taccagtggc aaactgggta gaaaatatca cagaatggtt
     5041 tacgacaact ttttctggcc tattttcatt tttacaaacg attggacagg cagtgatgtc
     5101 ggggattaca aatttactgt tagtgattcc agctcctttg tttatcttgt tattgacggt
     5161 agcagctttc tttatttcta agaaacgtcc aggacttacg ttgttcacat taatcggtct
     5221 atggtttatt tataaccaag gtctatggaa tgatttgatg aatactgtca cacttgttct
     5281 tttatctagt gtgatctcga tcatcattgg ggtaccatta gggatcttga tggcaaaaag
     5341 cagtaaagcc caaagtatta tcaaaccgat ccttgacttt atgcagacaa tgccaggatt
     5401 cgtttatttg attccagcag ttgcattttt cggtatcggg atggttccag gggtatttgc
     5461 ctcagtcatc tttgctctgc caccaaccgt tcgttttacg aatttaggga ttcgacaagt
     5521 tcctaaagaa ttagtagaag cttctgattc attcgggagc acaagtcgtc aaaaactatt
     5581 caaactagaa ttgcctttag ctaaaagtac gatcatggca gggatcaatc aaacaacaat
     5641 gctgtcactt tcaatggtcg ttattgcctc tatgatcggt gcacccggct taggacgcgg
     5701 cgtattatca gcattgcaaa gagcacaagt cgggaatgga tttgtcaacg gggttgcatt
     5761 agttatctta gcaatcatca ttgaccgttt tacacagcat ttgaaccagc caaacaataa
     5821 aaaagctgca ggagcaacaa cacagaaaag taaaaaacgt caaggactga tcattggtgc
     5881 tgtagtagta gtgatcttag gtgcgattgg tatagggagt ttttcatctg ccaaagaaac
     5941 aaaacgtatc aacttatctt atgttgaatg ggatacagaa gttgcttcaa caaatgtagt
     6001 cggtgaagta ttgaaacaaa tgggctatga tgtaacgatg acgccattag ataattcaat
     6061 catgtggaaa tctgtttcaa atggcgagtc agatgccatg gtttcagctt ggctacctaa
     6121 aactcatggt tcacaatatg cacaatataa agatcaagtc gaagacctag gtgccaattt
     6181 gacaggagca aaagttggtt tagcagtgcc agcctacatg gatgtaaatt cgatcgatga
     6241 gttaaccgat caggcaggta agaaaatcat aggaatcgag ccaggagctg gtgtagttac
     6301 ggctgctgaa aacacgatcc aaaaatatga caacttgaaa gactggaaag tagagacatc
     6361 ttcttcagga gcaatgaccg ttgcactagg tcaagcgatc aaaaaacacg aaccaattgt
     6421 agttacagga tggacacctc attggatgtt tgccaaatat gatctgaaat acttagaaga
     6481 tcctgaaaac ggaatgggaa gcgaagagca gatccatacg atggttcgaa aaggattgaa
     6541 agaagaccag ccagaagctt acaaagtatt agacaatttc cattggtcag aaaaagatat
     6601 ggaaaaagtt atgttggaaa tcaacaatgg aaaagatcca cagcaagcag caaaagactg
     6661 gatcaaagag aaccaagaat tagtcgaaag ctggaaaaaa taaaaagaca gacacgaaat
     6721 aaatggagct tccaaaattg gatcaaatat ccaaatttgg aagctccatg ttcgtctcta
     6781 tcttttttgt ctataaaaaa agagaatgcg tagctcatct tcgaaacgtc caaagacgcc
     6841 gacaaactat tatgaattta tgggtgcgag tcaaagagat cgatcgtagg tatgaaagac
     6901 taatgtgatt aaagtttatt atgtacgaag tgcattctca aaaaatatt
//
LOCUS       DAGCXG010000108         6829 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGCXG010000108 DAGCXG010000000
VERSION     DAGCXG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.113"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(277..534)
                     /locus_tag="MB022_002362"
     CDS             complement(277..534)
                     /locus_tag="MB022_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBT4467337.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(527..796)
                     /locus_tag="MB022_002363"
     CDS             complement(527..796)
                     /locus_tag="MB022_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4467338.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(927..1106)
                     /locus_tag="MB022_002364"
     CDS             complement(927..1106)
                     /locus_tag="MB022_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467339.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(1487..2194)
                     /locus_tag="MB022_002365"
     CDS             complement(1487..2194)
                     /locus_tag="MB022_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467340.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(2187..2624)
                     /locus_tag="MB022_002366"
     CDS             complement(2187..2624)
                     /locus_tag="MB022_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467341.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(2639..2890)
                     /locus_tag="MB022_002367"
     CDS             complement(2639..2890)
                     /locus_tag="MB022_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467342.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2890..3096)
                     /locus_tag="MB022_002368"
     CDS             complement(2890..3096)
                     /locus_tag="MB022_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBT4467343.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(3533..3793)
                     /locus_tag="MB022_002369"
     CDS             complement(3533..3793)
                     /locus_tag="MB022_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467344.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3868..4071
                     /locus_tag="MB022_002370"
     CDS             3868..4071
                     /locus_tag="MB022_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBT4467345.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(4212..4697)
                     /gene="ssb"
                     /locus_tag="MB022_002371"
     CDS             complement(4212..4697)
                     /gene="ssb"
                     /locus_tag="MB022_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4467346.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(4700..5599)
                     /locus_tag="MB022_002372"
     CDS             complement(4700..5599)
                     /locus_tag="MB022_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4467347.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(5612..6205)
                     /locus_tag="MB022_002373"
     CDS             complement(5612..6205)
                     /locus_tag="MB022_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4467348.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(6281..6430)
                     /locus_tag="MB022_002374"
     CDS             complement(6281..6430)
                     /locus_tag="MB022_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467349.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(6446..>6829)
                     /locus_tag="MB022_002375"
     CDS             complement(6446..>6829)
                     /locus_tag="MB022_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBT4467350.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQN
                     VAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKE
                     DFTTGFEFSEKKKTNYKKRTRRTTK"
BASE COUNT         1841 a         1360 c         1089 g         2539 t
ORIGIN      
        1 tatataagcg tcatccttta gtaactaaca tcccacacta caatagtatt ataacatatc
       61 tattcaactt caaattgttg aaatttaagc catttatatt tacttttcct caactttaga
      121 aatgatttta ctaaccttat atccgtaata cgcgtaacaa acacattcat taataacaat
      181 caacactaag ctatattagc tttttaaatg actagggtaa ataaaataag gatcaaaaac
      241 ttttaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt ttgcagaata
      301 aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg taattcttct
      361 ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc cagcaaaggg
      421 ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt tgtttccttg
      481 ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa tcatcagatt
      541 caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca tctcctcgac
      601 gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc atagaatcat
      661 aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt acaataagtg
      721 tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg aaatttgaat
      781 aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat tgtacattat
      841 attgtacaat taagcaaatg attaatattc taacttctta taattaaaag tgctatcact
      901 tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc aaatcctgct
      961 ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc acttttgccg
     1021 tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt cacgggtaac
     1081 cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa taaccctatt
     1141 aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac aaagcgccaa
     1201 aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg tagagctgca
     1261 ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat gattttattc
     1321 atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt cgcaccacct
     1381 aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct taatttcaag
     1441 gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat tgaccttttt
     1501 tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg atttcttctg
     1561 tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag tcgtcgcctg
     1621 ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg agttcataat
     1681 ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta aatacaaggt
     1741 aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct tccattaatt
     1801 ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc ctgcaaatcg
     1861 tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc atgatatatt
     1921 tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga cgacgtttat
     1981 acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca ctttccatat
     2041 acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa ctaaagtctt
     2101 tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat tgcgaaacat
     2161 cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga ttcaatcgta
     2221 taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct agccattgct
     2281 ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg atattcggaa
     2341 ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg gaaaaaccat
     2401 tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat gagattcatt
     2461 tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt gtaatgtttt
     2521 ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg tgccatttta
     2581 tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt tctccttttt
     2641 agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt tcttgataat
     2701 gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt gcatcctcta
     2761 ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct actttttcag
     2821 aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat aatttgtgtt
     2881 gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc cataagacaa
     2941 ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga tacatttcgc
     3001 tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga ataattcgct
     3061 gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct cctcttagga
     3121 aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag aatgaatcaa
     3181 cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct tgtcgctttt
     3241 tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata gcgaacagga
     3301 acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa gacaccatat
     3361 cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg gttggagtga
     3421 gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct tttcctgttt
     3481 ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc ttttatatgc
     3541 ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg actaattgat
     3601 tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc ataattaaca
     3661 atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga attgtttgta
     3721 aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca agcaaaccaa
     3781 catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta aaattgagaa
     3841 tttttattcg attttagata tgctataatg atcaagacag taaagtttgt gacggtggct
     3901 atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac agaaaggaga
     3961 agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc tttgatcgca
     4021 acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata accgtcagct
     4081 ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt cttaaacggt
     4141 gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac aactttattg
     4201 atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg tccgccaaat
     4261 ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg ttgttcaaag
     4321 gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc tgtcgctgct
     4381 ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac acgttgtcct
     4441 tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa actgcccttt
     4501 ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt aataaaatca
     4561 gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt aaaagtcgct
     4621 actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg tcctactaaa
     4681 gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt gatcttttaa
     4741 actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg tgatcaattt
     4801 cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag cgatattttt
     4861 cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt tcataataaa
     4921 aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt ttgtataact
     4981 ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa tcttttgctc
     5041 tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata atttgttctg
     5101 ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga tagtgtgtat
     5161 ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt acacgtactt
     5221 tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct tctcttgttt
     5281 ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta gttaaatcat
     5341 taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc gtatcgatat
     5401 caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg cctttcaaaa
     5461 tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc atcataaaat
     5521 gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt gtttttgctg
     5581 caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg tggtatttca
     5641 tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga tctctcaatg
     5701 ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat tcatatctcg
     5761 gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga tttccttgaa
     5821 aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa tgaaattgta
     5881 actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa gttaagtgaa
     5941 tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca tgagagaaga
     6001 ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga ccatcctcta
     6061 cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt tgtacagtgg
     6121 ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg cgaatagcca
     6181 acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa tacactacat
     6241 caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct aattcgattt
     6301 ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt gcacgtaatt
     6361 tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt ttgtgtgttg
     6421 tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt ttcttatagt
     6481 tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt aaaacgatcg
     6541 ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt gttcggccgt
     6601 tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc gttgtccata
     6661 atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc aagacttctt
     6721 gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt tcactggctg
     6781 ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtcc
//
LOCUS       DAGCXG010000109         6708 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGCXG010000109 DAGCXG010000000
VERSION     DAGCXG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.114"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..349
                     /locus_tag="MB022_002376"
     CDS             <1..349
                     /locus_tag="MB022_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467351.1"
                     /translation="GNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASI
                     NGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRITFS
                     KQKQPRREVQHSH"
     gene            complement(494..1234)
                     /gene="truA"
                     /locus_tag="MB022_002377"
     CDS             complement(494..1234)
                     /gene="truA"
                     /locus_tag="MB022_002377"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBT4467352.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1425..2060)
                     /locus_tag="MB022_002378"
     CDS             complement(1425..2060)
                     /locus_tag="MB022_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBT4467353.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2207..3448)
                     /gene="sstT"
                     /locus_tag="MB022_002379"
     CDS             complement(2207..3448)
                     /gene="sstT"
                     /locus_tag="MB022_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBT4467354.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3646..4644
                     /locus_tag="MB022_002380"
     CDS             3646..4644
                     /locus_tag="MB022_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467355.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4657..6675
                     /locus_tag="MB022_002381"
     CDS             4657..6675
                     /locus_tag="MB022_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBT4467356.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
BASE COUNT         2241 a         1288 c         1065 g         2114 t
ORIGIN      
        1 aggtaaccag gaaataaatc caacaactca ttttatgagt aacaaactta caattgaatt
       61 agataattcg aataataaaa atactgtctt gacgacacct atttatcgtt ataaagggca
      121 agtggcttct attaatggca aactagtaca aacaaaatta tctaagtttg gcacaactga
      181 attaactatt cctccaggta taaataaagt cgttattact tatcagtaca caaaactagc
      241 aattgcttca agatatttat ctatcgttac tttgatttta tttttacttt atcgaattac
      301 tttttctaaa caaaaacaac cgagaagaga agtacaacat tctcactaga tttttattta
      361 agaaagactt tttagctgta cacataaaca ggctaaaaag tcttttttta tatttcttta
      421 atttgcttca tacattattt aacatcgata tttatactgt gaaacatgcg tttcatccat
      481 tatcaaagta tttttatcga taataaactt catccaaaaa cagaccttgt gcagggacgg
      541 tttctcctgc atgttgccgc actttgcttt caaatatttc atcgattgtt tctatcggca
      601 tggcacctgt gccgatttct agtaatgtcc ccatgataat ccgcaccatt ttatataaga
      661 atccgtctcc gacaaaagta aaatgaagca tttctccctt tcgttcgata gataattcat
      721 caatcgtacg cactgtggat ttttttgatt ttttcaatgc agaaaaacca atgaaatcat
      781 gtgtacccac taatttttcg caagctgcat tcagcttgtc catatccaac tcttgaggga
      841 aataaaagct gtggtttcgc tcaaatgctg aaggaatcac gttgttccaa acataatagc
      901 tgtatttttt gcccaccgca ttatatcttg aatggaagcg ctcaggcatt tcttcgattc
      961 ttttgatgac aatatcccgc ggaaggtaac gattcataaa gtcgatcatt tctgccagtc
     1021 caatttctgt gtttgtttta aaatttgcta cttgtcctcg cgcatgggtg ccagcatctg
     1081 ttcttcctga tccaataatt tctatctctt catttgtcat ctgatgcaat actttttcga
     1141 tttttccttg aatggtttta tcgtcatctc caagtctttg ccatcctgaa tatctttttc
     1201 cgtcatattc gattgtcaac ttgatgtttc tcatatttta gtggctcctc tgtcttccat
     1261 ttggtcttct ctctaccttt ttaagattgt agcacaaaat atagctcctt gcttctctaa
     1321 gacagaagct atacaagaaa actgtggaaa tatatatttc agaaaaacaa aaaacagcca
     1381 ccggatgaag tatccgctga ctgttcaatt tgacggctca agttttactg tttgagatgc
     1441 aagatactca accaggcaat gggcacataa ttaaagaaaa aactgattct ttggcaaaaa
     1501 cctattttat tcagtaatgg ccaagaattt atagaaggaa ttcttgccag tccataaatc
     1561 gccgcaaaaa gcaaactgat cacagacagt ccaaggtata agtggcttac gtccatttgc
     1621 ccattttttc gatagatcaa gaaaagaaga actggaaaca ataaaaaacc actgtaacct
     1681 aaacctgatc cgatattatg gacccatgtc gagacattcc acgaagattc atgggtattg
     1741 atactgaaca aacctgtaaa gatacaatca cctataccat agaacgcgat ggctgctgta
     1801 actagggtag ctaacacacg agatgtacta acaaatgcag catacaatgc aggcaatgac
     1861 aggatgaaaa agatccctga aaccacagac caaataagaa acgcttttct tacaggactg
     1921 gaaacctctc caaatacact gatcacgctc ttcatttgat ctaattcagg ataatacaat
     1981 cctaatacgt acggagtcaa aaaatcactc agcacgccta ataataaaaa gaaaaaacca
     2041 aatttacgta aaaaatccat gatacgcctc caataacaat tacctatgct tttaattgtt
     2101 attataaacg actttatctt gatttaacag aaacctctat ttacacgaac atctatataa
     2161 ctcattaaaa actcgaatca ttttaaaaat actaaatatc taacgttcaa cgttcactta
     2221 atccatctaa acaatctttc aatttgattt tttccccact tcttttacgc gcgcctagct
     2281 ctcctattgc agtaaacaac agatcacttg acgaatttaa ggcagtttcc attgaatcct
     2341 ggataacacc gataataaat ccaataccga cgatttgcat cgcgacatca ttagagatac
     2401 caaacaaact acatgctaat ggaatcaata acagtgaacc gcccgcaaca cctgaagctc
     2461 cacaagcagc taaagcagac aaaatacata aaaggacttt caaaacaaaa ggtgcttcaa
     2521 tccctaaaga atgtacagca gtcaacgtca tcaaggaaat ggtaatcgcg gcaccgccca
     2581 tattgatcgt agcacctaac gggatcgata tggaataaga ctcttttgtc aatttcaatc
     2641 tttcagccaa catcatgttt atcgggatat ttgcggcaga acttcgggta aagaaagcgg
     2701 gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata gggattttct ttaataaaca
     2761 aaaacgtgag gaatgggtac acaactaatg cgacaaacag catcgtaccg atcaaaacaa
     2821 gtaaaagctg cagatactct gccaatccag cgattccagt cgtagcaatt gattgataaa
     2881 ccaatcctaa aatccctatc ggagcaaatg caataatcac ttgtacgact tgtgacaacg
     2941 ctatagatat ctgatccacc aactcttttg tcgattctga acgtgctcga aaagctagtc
     3001 ccaacaatac agcccagaat aagacgccaa ggtaattcgc atcaataaga gattgaacag
     3061 gattttgtgt gacattcgtc aacatagtag ataaaacact ttccaaactt tgcggtgctt
     3121 tttcagatac tgtttcttgt aaagtaatag gaacagtaaa aattgaactt acaataactg
     3181 ctacaagtgc agaaagcaat gttcccacta aatataagat caaaataggc ttcacgaatg
     3241 tcttattccc aattttatgc ttggcaattg acgccatcgt caagaaaaaa accagaatag
     3301 gtgcgattgc cttcaagcaa ctgacaaaca acgttcccaa tattgaaata aatgtccatg
     3361 tggggaaaaa gataccaaac aatattccga ctaaaatacc aacaacaata cgttgaacta
     3421 acgacatatc caaaaacttc tctaacaaat gaccaccctt tcacaatttt aacaatcatt
     3481 gtatcatttt atgcaaagaa actaaaggta taattttcta aaaattctaa caatacaaac
     3541 aaccatttct tgctaatttt gtactttttc tgtctacacg ttgttttttg agatattcta
     3601 agttaggatg ttattaatgg gcaacttatc aggaaacgtt ttcttatgtt tttgtccaat
     3661 ttttttgtga ggtgtttatt cttgaaaaaa ataaagaagg agagttatgg cttcctgaca
     3721 atctttgtcg cttgtataat gatggtttta cagcgtatat tgatttcagc agtcactatt
     3781 cctacatatg caacaaaccc tggtcgattc tacatctata catttttaca aacttccgtt
     3841 gttattggtg caaatttgat cccactttac atgggttacc aatatcaaaa aatcgcccgt
     3901 ttaaaagttt tacattattt aagtcgtttt gcctttatat tcctaatagc cacactagtt
     3961 tgcaatatct ttttttacgt acaagctggc agcttgaata ttcgtgatta ttggcttatc
     4021 ctaacaccta tcagtcagaa ttactttact tatgctgttt cttgtacttt gcttttttgt
     4081 agtattcctt atacagttgg atttttaaat aaattatcga aaacaacaat taaatatcta
     4141 ctattatttt taacaattgg attagttggc tgctctactc tatttaataa agacccgttt
     4201 gcattacaga atggacagag tattttatgg attttttatt tatttttaat gggttatggt
     4261 ttgaaagaat ggaactggaa agaaaaggtc cgctataagc ttcctcaagt atttatcagc
     4321 ttgattgttt tattcggttt gattattttg atgacacaaa tttcactcat catcagagga
     4381 aatacagata cagcgctgag gttttctaaa ccatattctc tcttcgcaat gtattatact
     4441 ttctcttcgt ttttattttt agaaatgcta tctgataaat taggtataaa aggaagagga
     4501 aattttcttg ctgtattttt gattgtcact caagttatat gtaatttccc tttagtttct
     4561 tatcgcgtat ctacatttat gaaacgtgaa tttccaaact cagggaaggc atggctgata
     4621 aatatcgggc agttttttat ttgatattta ttagcagtgt ccctcctagt tatcttttta
     4681 ttttatatac aaaaaatccg tttcattcat cgtttcatcg aatacttcgc ttttgattcg
     4741 attgaacaat taactcagcg ttggaaaaaa agaattcatt ggctttttgt tcataaaagt
     4801 tattttttag ttgcagcatt tttttactgc tttaccttcg tgcagatttt cgttttagaa
     4861 ccaaaagaag gttggaaaga aactatccag gttgccttaa aaatatttac tcagcgacaa
     4921 gcacccatga tcctgacaat catcattatt atgggatttt tcttcttact attgttgatt
     4981 tccaatcgtt tttggtacgc cttgactgct accttgatca ttaacttact gctcactatt
     5041 tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct ttccttcaga tttaaaaatg
     5101 ctgaccggat tgtcagaact attatcgatg gttagcccag tcttattaat cgtgggcgga
     5161 ttgatccttc tcctattatt gattactagt attattgtac aacgtagact acaaaaacag
     5221 tattccttga agattcattg gaaacgtcga tttatcagta tcgctgtatt attaggatgt
     5281 ttttcgggtg tctttttcat taatcacaaa aattcgcctt ctttcttgct gtttaattta
     5341 tttaaagtaa acaagacttt ctttaaccag caaggcgccg taaaagataa cggaccagtc
     5401 attcaattcc taaacaatat cgacattaaa atcatggaag aacctgctgg ctattcgaaa
     5461 gaaaaaatta atggaataat gaaaaagtac aacacaaagg cagatgaaat caaccaaaca
     5521 agaagtgact ggttaaacaa tcagacgatc gttctcaatt taagtgaaag cttcagtgat
     5581 cctgcacgtg tccctaatct gacagtacct acagacccta ttccttacat caaaagcatt
     5641 ataacagaag gaacaggagg attgatgtta tccgtcggtt atggtggcgg aacagctaac
     5701 atggaatggg aaggactgac aggcttgagt atcagtaacc tgtctgcttc gttagttaca
     5761 ccttataccc agctagtgga gcgtcagaaa ataagtccaa atattaccga tctattcgat
     5821 gaaaaaatag ccatccatcc ttttacagct gctctttatc gcagaaaaca agtgtttgat
     5881 aaatttggtt ttgacaggtt ctattattta gacagtccgg ataaattaac ttatacggat
     5941 aaaatagaac aaagcccacg catatcggat gattcagcat acaaagaaac cttgaaagct
     6001 ttacaaagca atacggaaac gtcacaattc atccaattat ccactatgca aaaccatatg
     6061 ccttatgatg attactatag tgaattgaac tacactgctg aaggaactgc tgtcattcct
     6121 aatagaagaa aagaattaca aacttatatg caaggccttc actataccga tacagcagta
     6181 aaaaaattca tccaagaaat cgacaaaatc gataaaccga ttacctttgt tttttatggt
     6241 gaccatttac ccgcaatcta tagtgggaat gattcgaaaa aatatggatt agaacagcac
     6301 cagacagatt atttcattta taacaacaag tatagtcgtg aacaagcagc tacgttaaat
     6361 aaagaagttg ttgctcctaa taactttggt gctatggctc ttgagcatgc gaatatcaaa
     6421 gtaactcctt actatgcgtt gttgacagaa gtatctaatc atctacctgc attaacaatt
     6481 gatcctacag aaagcttgtc taatcgtttt aatggaaaac aggtctttgt tacagaggat
     6541 agtcaaatta tccaagaaaa aaatttaact gagaaacaaa aagaaattct agaagattat
     6601 cgtctgatac aatatgatct tgttgcaggt aaccagtaca gtgcaacatg ggcgacacaa
     6661 aaaataaaag actaacaaat aaactgagca aggggttgtg ataaaaga
//
LOCUS       DAGCXG010000110         6693 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.115, whole genome shotgun sequence.
ACCESSION   DAGCXG010000110 DAGCXG010000000
VERSION     DAGCXG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.115"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..142)
                     /locus_tag="MB022_002382"
     CDS             complement(<1..142)
                     /locus_tag="MB022_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467357.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="MB022_002383"
     CDS             complement(183..437)
                     /locus_tag="MB022_002383"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4467358.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="MB022_002384"
     CDS             complement(548..802)
                     /locus_tag="MB022_002384"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467359.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="MB022_002385"
     CDS             complement(995..1270)
                     /locus_tag="MB022_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBT4467360.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="MB022_002386"
     CDS             complement(1242..2195)
                     /locus_tag="MB022_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4467361.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="MB022_002387"
     CDS             2807..4300
                     /locus_tag="MB022_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBT4467362.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="MB022_002388"
     CDS             4435..4731
                     /locus_tag="MB022_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBT4467363.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="MB022_002389"
     CDS             complement(4755..5714)
                     /locus_tag="MB022_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4467364.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6229
                     /locus_tag="MB022_002390"
                     /pseudo
     CDS             5897..6229
                     /locus_tag="MB022_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6392..>6693
                     /gene="tnpA"
                     /locus_tag="MB022_002391"
     CDS             6392..>6693
                     /gene="tnpA"
                     /locus_tag="MB022_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBT4467365.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT         2151 a         1138 c         1175 g         2229 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa cagaattagg
     6241 cagaagtcgc ccatcctcta taggtgggtg atgaatgccg ttcggtatga gggttaccgt
     6301 tggtaactcg aagaccgaca actaaggcgt attgcctttt tattttgttc catgttacaa
     6361 tcagtctata caagaaatga gttgatataa catggaatta gatacaaata atcattcagt
     6421 atttcttctt tattaccacc ttgttttagt aacgaaatat cgtagacaag tgattgatga
     6481 tgaaatatct gattatgcca aaactacttt tgaaagaatt tcagaatcgt atcacattac
     6541 tttagtggag tggaatcatg ataaagacca cgttcatatt atgttcaaag ctcaacctaa
     6601 aacagaattg acaaaattca tcaatgccta taaaagtgcc agctcaagat tgataaaacg
     6661 tgactttcca agagtcaaac aatttctttg gaa
//
LOCUS       DAGCXG010000111         6563 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGCXG010000111 DAGCXG010000000
VERSION     DAGCXG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.116"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            188..964
                     /locus_tag="MB022_002392"
     CDS             188..964
                     /locus_tag="MB022_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467366.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNIS"
     gene            1001..1945
                     /locus_tag="MB022_002393"
     CDS             1001..1945
                     /locus_tag="MB022_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4467367.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLNKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1961..2740
                     /locus_tag="MB022_002394"
     CDS             1961..2740
                     /locus_tag="MB022_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBT4467368.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKEAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPISPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNGKTAPSRRSRSRV"
     gene            2752..3450
                     /locus_tag="MB022_002395"
     CDS             2752..3450
                     /locus_tag="MB022_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBT4467369.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLSNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQIFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3478..4242
                     /locus_tag="MB022_002396"
     CDS             3478..4242
                     /locus_tag="MB022_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBT4467370.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQVELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLIPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4306..4995
                     /locus_tag="MB022_002397"
     CDS             4306..4995
                     /locus_tag="MB022_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4467371.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRCIYLFFKRMIDIIGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTQIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSGRNDVSFHAMVDLDLEYITNQSLLNDFKLIIKT
                     IWIVIKPNGAY"
     gene            5009..5458
                     /locus_tag="MB022_002398"
     CDS             5009..5458
                     /locus_tag="MB022_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HBT4467372.1"
                     /translation="MKICLVGSSGGHLTHLYLLKNFWEKEERFWVTFDKEDSRSMLKD
                     ERFFPCYYPTNRNIINLIRNSFLAIRILLKEKPDLIISSGAAVAVPFFYLGKLIGAKT
                     IYIEVFDRIDAPTLTGKLVYPVTDKFIVQWDEMKKVYPKAINLGGIF"
     gene            5458..5949
                     /locus_tag="MB022_002399"
     CDS             5458..5949
                     /locus_tag="MB022_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HBT4467373.1"
                     /translation="MIFVTVGTHEQQFDRLIKEIDRLKESRLISEEIFMQTGFCNYIP
                     KTCDFQQFLSYEQMEEYIDRARLVITHGGPSTFMNVLKKGKKPIVVPRQKRFSEHVND
                     HQLEFAEAVKKKGYDIYVLKEINELLNLLNNSKNDIEIKVNSNNLKFCEKLESIVNSL
                     DIK"
     gene            5977..>6563
                     /locus_tag="MB022_002400"
     CDS             5977..>6563
                     /locus_tag="MB022_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBT4467374.1"
                     /translation="MIPKKIHYCWFGKNPLPNEVKKCIESWKLFCPDYEIIEWNEENY
                     DVFKIDYMAEAYKAKKYAFVSDYARLDIIYNNGGIYLDTDVELIRPLDDLLHHSCYLG
                     MELPGRVATGLGFGAVKGNNFLYENMKYYEHNHFFYKGRMNLTTCVDITTNILKKHGL
                     SNDDNIQIIKDTIIFSTEYFCPYDIEKGITNLTCNT"
BASE COUNT         2352 a         1047 c         1336 g         1828 t
ORIGIN      
        1 acttttaaat cgtaaattgc cctagtcagg aaaacagttt gtggtatgat tatataggga
       61 aaacttgttt acaaatattt ctgtgaatat attagtttaa ttatgttttt taaaataaaa
      121 agctgtcatt tttatgtaag tgaattatat cttaatttat ctatcatcaa tacttaggag
      181 tgaatctatg gaaaagccat tcgtcaagtc attgactgct gtggcggtag gtgcaggtgc
      241 tattgcgatc tgtttgtttg gttaccatat caataatcag cggcaacatc agcaacggat
      301 caattatgca gaatctgcca tcaccaatca aaaagacacc ttgacttctt tatcaaaaga
      361 agtagacaag ctgtatagta caaaagaaaa aatttttcta aaccctgaaa taacagaaga
      421 aacaatcaca agtttgtcaa ataagctatc ctctgtcaaa ctttcagcag atgattacga
      481 catcaaagaa tcagaattgc ctaaagaagc tgttgcaatc caagatgaga agaaagcaat
      541 cgtgacacag ttagatagtg cagaatcaaa actgaagatc cagacagctg tcaacgattt
      601 gtttactaaa aatgtatcaa actggcagca agctgtagac gatgtcatca tcaaagaaaa
      661 ggtgacaagt gcagatgtcg ctcgtgtacg tgaaaatatg agctttttta aagatagtcc
      721 ttggaaaacg gttgttatgc aatatctagg attcgctgat actcagatcg cgcaagtcac
      781 gcaactagat cagttattcg atacgatgtt aaaggatggt caagtgactg ccaccgctac
      841 ttacgatcaa tatttgacgg cgctcagtca aatagagcag atccggaatg aaaaaataag
      901 cgcagcatac gcaacaaaag cacaaactgt tgctcagcaa atgggctata gcaatataag
      961 ttagtaacaa ttaaagcaaa gctagcaaac ggaggaaact atggcaagac gaagaagaaa
     1021 atcaagaggg aaaaaaatat ttctgattat tttagcagtt cttatcgtat tagtattagc
     1081 aattgtagga gtaggagcaa aattatatct ggatctctca cattctattg agaaaactta
     1141 cgagtcagtg gaacgaaccc aaggagaacg aacgaaacca gttgatctga acaaacaaga
     1201 atctttctct gttctattga tgggcattga tactggagat ttaggtcggg tcgatcaagg
     1261 aagatcagat acgatgatgg ttgccacagt cagtcctgaa gacaaacaga caacgatcgt
     1321 cagcattcct cgagatacgt atgtcgacat cgtaggtcgt gacacaaaag acaagatcaa
     1381 ccacgcttac gcattcggcg gagctgccat ggcgatggat accgtacaaa aatatgtaga
     1441 catcccaatc gatcactatg tctctatcaa tatgaaaggg ctaaaggaac tcgttgatgc
     1501 agttggtggt atcgaagtca ataatgacct cactttctca caagatggct atgactttcc
     1561 aatcggtaaa attacattga acggggatca ggcattatct tattctcgta tgcgttatga
     1621 agatccaaat ggtgattacg gtagacaagg gcgccaaaga aagatcgtag aaggcgtagc
     1681 aaaaaaagta ttgagtctaa atggtgtcag caactatcaa tccgttttga atgctctaga
     1741 gtcgaatatg aaaacagaca tgagctttga tgaaatgaaa aaaatcgctt tagattatcg
     1801 ggaggcattt ggtacgatca agcaagatca gctgcaagga gaaggcttca tgcaagataa
     1861 tatctcctat caaaaagtca gcgatcaaga acttgatcgt gtgaaaaaag aattaaaaga
     1921 tcaaatgaat ctagagaata aataggaaag tggagaaaaa atggaagaga caatcagctt
     1981 gaaagaaatt tttgaaacat tacgcaaaca cttggtaaca atcatcatct caatgtttgc
     2041 tggcttggcc atctcaggaa tcgtaacatt tttcatcttg acaccaaaat acagttcaca
     2101 agcacaattg atcgtgacac tgccacaatc agacacagca aatgtcaatg atgtcaatac
     2161 gaacctgcaa atgatcaata cgtataaaga tatgatcgtc agtgatttag tgctaaatga
     2221 agtcaaagat cgtttagaaa cagaagacaa tgtgaaaatg actgctgaac aaatcaaaga
     2281 ggccatctca gtcaatcagt cacaaaattc acaaatgttc tcgatccagg caataagcac
     2341 gaatgcagtg actgctcaac agatcgctaa cacaacttca caagttttcc aagagaacgc
     2401 aaaagacgtg atgaatgtcg ataaaatctc gattatttcc ggtgcagttg ccaatacgac
     2461 acctatttcg ccaaataata aattgaattt agtgattggc ttagtattag gaatgatggt
     2521 aggcgtaggt ctggcattct tattcgaatt actcgatcgg acagtgaaag atgaaaaata
     2581 tgtgacggat acattaggat ttccaatctt aggaacagtt ccagaaatgt cagcgaaaga
     2641 attgaacgct ggtatccgta aaaaaggatc agctcctgca cgaccaaaag ctacaaatgg
     2701 taaaacagcc ccatcacgca gaagccgctc aagagtctag gaggaagaaa aatggcacga
     2761 acacagaaac aaaaagcagc acaaactcat gcagtcagtt tgatcacatt aacaaaccca
     2821 tcttcaccaa tcgctgaaca atatcgtacg atccggacga atatccagtt tgcctccgca
     2881 gctggtcagc aaatcaaaac gattgtcgtg acttcatcag gtcctggaga aggaaaatcc
     2941 accactgcag caaacattgc agtcgttttt gctaaatccg gtcaacgggt tctattagtc
     3001 gatgcagatt tacgtaaacc cgtcgtctat aaaacattcc agttgagcaa tgctagtgga
     3061 ttaagtacag cacttagttc atcaggaagt gtagcagatg tgatccaacg aacgccagtc
     3121 gagaacttgt cgattttgcc tagtgggcct aagccaccca acccatcaga attgttaagc
     3181 tcgccaagaa tggatcagat tctagccgaa gcaagacaga tatttgacgt ggtgatattc
     3241 gatatgccac cagtagtagc cgtaacagat gcacagatca tgtcttcaaa aacggatggc
     3301 actttattgg tcgtacggga aaatacatca cgtaaagaat ccttgactaa agcaaaagaa
     3361 ctgctagaga tggtacaagc acgagtctta ggtgttgtat acaacggagc agaacatagc
     3421 aaagatgctg gttattacta cggaaattaa ggaataagaa gagaggggac aagagaaatg
     3481 atcgatctac attgccatat cctaccagga atcgatgacg gggcagaaaa cttagaagca
     3541 agtattgcaa tggctgaaaa agcgattcag caaggcatca cgcatatttt atgcacacca
     3601 caccataata atggaaaata cagcaatgaa aaatcacaag tcatttctct agtcgcttcg
     3661 ttacaagtag aattagagaa aagacaattg cctttgacgt tattggaagg acaagaagta
     3721 cgtattaccg gaacgctcat cgaagacatc catcgagatg aaattttatt tacagattta
     3781 gatgatacat atcttctgat cgaatttccg acactggaag tgccgttata tgcagaacgg
     3841 cttttttttg agttgttaga actaggaaag acaccagtga tcgtccatcc agaacgtaat
     3901 gcgcattttc gcgaagaccc gaatcatttg attccgttcc tagatatggg atgtttggcc
     3961 caacttactg cgccaagtta tgttggtgta tttggcaaag atatccagaa aacagccaaa
     4021 acgatggtca aacataatct tgtgcagatg gctgcttctg atgcccatgg ggtctctaag
     4081 cgcacctttt acttaaaaga gtgctatgaa cagattgcca aggagtttgg taaggaaaaa
     4141 gtagtacaga tgaaacaagt cgcaaaggat ctgatcaacg gggatgagat caattatcca
     4201 atctatgaag aggtaaaaaa gaagaagttt ggtttatttt agttctttat tcgtagataa
     4261 aaaggaaatg aggagagagt gaagatagtg aaagggactg aaagtatgga aagtgaagat
     4321 ttattatctc gtattagtga agtacatcct aatcaagttt ggttggataa agaaaaagta
     4381 gaaaaacgat gtatctattt attttttaaa agaatgattg atattatagg aagcattatt
     4441 ggtttgattt tagcttctcc tttgatgttt tatgtagctt atcgaatcag aaaagaagag
     4501 ccaggaagtc caatattttt tagccataca cgtgttggta aaaatggaaa aacattcaaa
     4561 atgcataaat tccgttctat gtgtttagat gctgaagaaa aattggaaga gttacttgat
     4621 caaaatgaaa tagaaggcgc aatgttcaag atgaaagaag atccacgtgt tacccaaata
     4681 ggtaaaatta tccgtgctaa aagtattgat gaattgccac agctatggaa tgttttaaaa
     4741 ggagatatga gtctaattgg cccacgtcca cctttggaaa gggaagtagc tgagtacacg
     4801 agatatgatc ttcaacgctt gtatgtaaaa cctggatgtt caggtttgtg gcaagttagt
     4861 ggtagaaatg atgttagttt tcatgcaatg gttgatttag atttagaata tataactaat
     4921 caaagcctat tgaatgattt taaactaatt atcaagacaa tttggatcgt tataaaacca
     4981 aatggggcat attaaggagt gtaagttatt gaaaatttgt ttagtaggtt caagtggagg
     5041 acatttgact catctatatt tattaaaaaa tttctgggaa aaagaagaaa gattttgggt
     5101 aacatttgat aaagaggatt cgagaagtat gttgaaagat gaacgatttt ttccatgcta
     5161 ttatccaaca aatcgtaata taataaactt aatccgtaat tcttttctcg ctattagaat
     5221 actattaaaa gaaaaaccag atttaatcat atcttccggt gcagctgtag cagttccgtt
     5281 tttctattta ggtaaattaa tcggagcaaa aactatttac attgaggttt ttgatagaat
     5341 tgatgcgcca accctgactg gaaaattagt atatcctgtg acagataaat ttattgtaca
     5401 gtgggatgag atgaaaaaag tgtatccaaa agcaattaat ctcggaggta tattttaatg
     5461 atatttgtga ctgtgggaac acatgaacag caatttgatc gtctaataaa agaaattgat
     5521 agattaaaag aaagcagatt gatttcagaa gaaatcttca tgcaaactgg gttttgcaac
     5581 tatataccta aaacttgtga tttccaacaa tttttatctt atgaacagat ggaagaatat
     5641 attgatagag ctcgtttagt aatcactcat ggtggcccct ctacatttat gaatgtttta
     5701 aaaaagggga aaaaacctat agttgttcct agacaaaaac ggtttagtga acatgtaaat
     5761 gatcatcaat tagagtttgc tgaagcagtt aaaaaaaaag gctatgatat ttatgtactt
     5821 aaagagatta atgaactttt aaatttatta aataattcta aaaatgatat agaaattaag
     5881 gtaaatagta ataatttaaa gttttgtgaa aaactagaaa gcattgtaaa tagtttggat
     5941 atcaaataat ttttagagaa ggaggaattt taaagtatga tacctaagaa gattcattat
     6001 tgctggtttg gaaaaaatcc acttccaaac gaagtaaaaa aatgtataga atcatggaag
     6061 ttgttttgtc ctgattatga gattattgag tggaatgaag aaaattatga tgtattcaaa
     6121 attgattata tggctgaggc ttacaaagca aaaaaatatg cttttgtttc tgattacgct
     6181 cgattggata ttatttataa taatggtggc atatatttag atacagatgt agaattgatt
     6241 agaccattag atgatctttt acatcattct tgttatttgg ggatggagtt accgggtaga
     6301 gtcgctactg gattaggttt tggtgctgtc aaaggcaaca atttcttata tgaaaacatg
     6361 aaatattatg aacataatca ttttttttat aagggtagaa tgaatcttac aacttgtgtg
     6421 gatataacga ctaatatttt aaagaaacac gggctatcta atgatgataa tatacaaatt
     6481 atcaaggata cgataatttt ttcaacagaa tacttttgtc cctatgacat tgaaaaaggt
     6541 attacaaatc ttacatgtaa tac
//
LOCUS       DAGCXG010000112         6463 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.117, whole genome shotgun sequence.
ACCESSION   DAGCXG010000112 DAGCXG010000000
VERSION     DAGCXG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.117"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(105..560)
                     /locus_tag="MB022_002401"
     CDS             complement(105..560)
                     /locus_tag="MB022_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467375.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="MB022_002402"
     CDS             complement(714..1922)
                     /locus_tag="MB022_002402"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBT4467376.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="MB022_002403"
     CDS             complement(2034..2366)
                     /locus_tag="MB022_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4467377.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="MB022_002404"
     CDS             2514..3389
                     /locus_tag="MB022_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4467378.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="MB022_002405"
     CDS             complement(3471..4052)
                     /locus_tag="MB022_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBT4467379.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="MB022_002406"
     CDS             complement(4058..5317)
                     /locus_tag="MB022_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBT4467380.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="MB022_002407"
     CDS             complement(5319..6365)
                     /locus_tag="MB022_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467381.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1801 a         1337 c         1102 g         2223 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaa
//
LOCUS       DAGCXG010000113         6173 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.118, whole genome shotgun sequence.
ACCESSION   DAGCXG010000113 DAGCXG010000000
VERSION     DAGCXG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.118"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            725..811
                     /locus_tag="MB022_002408"
     CDS             725..811
                     /locus_tag="MB022_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4467382.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="MB022_002409"
     CDS             complement(932..1621)
                     /locus_tag="MB022_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467383.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="MB022_002410"
     CDS             complement(1628..1906)
                     /locus_tag="MB022_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467384.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="MB022_002411"
     CDS             complement(1866..2780)
                     /locus_tag="MB022_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4467385.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="MB022_002412"
     CDS             complement(2762..3142)
                     /locus_tag="MB022_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBT4467386.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="MB022_002413"
     CDS             complement(3592..3879)
                     /locus_tag="MB022_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4467387.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="MB022_002414"
     CDS             complement(3899..4123)
                     /locus_tag="MB022_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBT4467388.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="MB022_002415"
     CDS             complement(4578..4748)
                     /locus_tag="MB022_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467389.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="MB022_002416"
     CDS             complement(4763..5296)
                     /locus_tag="MB022_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4467390.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="MB022_002417"
     CDS             complement(5317..6159)
                     /locus_tag="MB022_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4467391.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAGCXG010000114         6147 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.119, whole genome shotgun sequence.
ACCESSION   DAGCXG010000114 DAGCXG010000000
VERSION     DAGCXG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.119"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(360..845)
                     /locus_tag="MB022_002418"
     CDS             complement(360..845)
                     /locus_tag="MB022_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467392.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(984..1229)
                     /locus_tag="MB022_002419"
     CDS             complement(984..1229)
                     /locus_tag="MB022_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBT4467393.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1422..2222
                     /locus_tag="MB022_002420"
     CDS             1422..2222
                     /locus_tag="MB022_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4467394.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2241..4109
                     /locus_tag="MB022_002421"
     CDS             2241..4109
                     /locus_tag="MB022_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBT4467395.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4102..4593
                     /locus_tag="MB022_002422"
     CDS             4102..4593
                     /locus_tag="MB022_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBT4467396.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4581..5135
                     /gene="srlA"
                     /locus_tag="MB022_002423"
     CDS             4581..5135
                     /gene="srlA"
                     /locus_tag="MB022_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBT4467397.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5153..>6147
                     /locus_tag="MB022_002424"
     CDS             5153..>6147
                     /locus_tag="MB022_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBT4467398.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2174 a          976 c         1227 g         1770 t
ORIGIN      
        1 atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa
       61 taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa
      121 aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa
      181 gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa
      241 tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta taactttctc
      301 agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat
      361 caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa
      421 aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa
      481 taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa
      541 cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca
      601 gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta
      661 tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta
      721 gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt
      781 ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta
      841 tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa
      901 aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata
      961 acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg
     1021 aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa
     1081 atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac
     1141 agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa gttttcatta
     1201 gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac attgcttata
     1261 taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt gctcttccat
     1321 tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt tagtgagctt
     1381 ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat tggttggata
     1441 ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga agtatcgtcg
     1501 aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa tgccgtatac
     1561 agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac atagaagaag
     1621 ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat aatgcaggaa
     1681 taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat gaattatcag
     1741 aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg atgagccaag
     1801 cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt tcttctgaat
     1861 caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca gcgatgaaca
     1921 gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg
     1981 caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa gctctttctt
     2041 atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca agtacgatcc
     2101 ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat tatctttcag
     2161 aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa acaagaggat
     2221 aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta ttgaaattac
     2281 tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc
     2341 aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt gcaaaaatca
     2401 tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt gatgagatca
     2461 tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga atcgcttatt
     2521 tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg tctgaagaag
     2581 tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag atgatcaatg
     2641 attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata ggagatgagc
     2701 tagaattacg actactttta ttttattttg tttctcccta cttttcagag acgaaaacag
     2761 aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg aatgctagta
     2821 aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga atctctagta
     2881 actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat atccaagaat
     2941 tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc caatatgaaa
     3001 aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg ataaggaaag
     3061 ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga cgattagatc
     3121 tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc tctttattga
     3181 taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag atagaaaaaa
     3241 agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa gctatcttgc
     3301 aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag ttagcatttc
     3361 gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg acaggaaaag
     3421 gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat atacaaataa
     3481 cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc gctgttttta
     3541 caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg actaatcttt
     3601 ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt
     3661 ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat caagaatatt
     3721 taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa
     3781 ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt gcttttcctc
     3841 acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata actgatcaag
     3901 gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct gaagaaatga
     3961 cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa ctcataaaaa
     4021 tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta aaactaatca
     4081 aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt cgcagcttca
     4141 gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa tcaaacatat
     4201 agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa aatccgttca
     4261 ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt cagaaaaatg
     4321 caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg tgaagatatc
     4381 cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac acaagcagct
     4441 ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa aagtgaacaa
     4501 ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag tacgatcaaa
     4561 gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga tttatgaggc
     4621 tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta ccagttgtct
     4681 tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac cgagtaacga
     4741 aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat
     4801 ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt ccagaatact
     4861 acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat ggtgtattcc
     4921 cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg atcgaaacct
     4981 tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg ctgatgaact
     5041 ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag caaggaatcg
     5101 ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt aaatgtatgg
     5161 tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta ttaggccgac
     5221 aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa tgatcgtaga
     5281 taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat caatacctga
     5341 tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg gaatttatcc
     5401 taaaaaaggg ataccaacca tcaatatcgt tcccacagga aaaagtggcc cattagcaca
     5461 atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac agccactaat
     5521 ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa agtacaaata
     5581 tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga tagcaaaaat
     5641 tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag aagcagtaga
     5701 tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga ttggtgtgat
     5761 acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag caggaaatgc
     5821 agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc ctttgcttgg
     5881 tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga ttgggaaagg
     5941 aaatattcct ccccaattag cattaccggc actatttgct atcaatactc aaaatgcatg
     6001 tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag tagaagtagg
     6061 tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta tcattgcttg
     6121 ggtagcaagt tttggattat atagctg
//
LOCUS       DAGCXG010000115         6066 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGCXG010000115 DAGCXG010000000
VERSION     DAGCXG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.120"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            832..1566
                     /locus_tag="MB022_002425"
     CDS             832..1566
                     /locus_tag="MB022_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4467399.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="MB022_002426"
     CDS             1582..2751
                     /locus_tag="MB022_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4467400.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="MB022_002427"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="MB022_002427"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4467401.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="MB022_002428"
     CDS             3841..4767
                     /locus_tag="MB022_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBT4467402.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="MB022_002429"
     CDS             4786..6036
                     /locus_tag="MB022_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4467403.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1245 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt aaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaa
//
LOCUS       DAGCXG010000116         6041 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.121, whole genome shotgun sequence.
ACCESSION   DAGCXG010000116 DAGCXG010000000
VERSION     DAGCXG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.121"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(33..749)
                     /locus_tag="MB022_002430"
     CDS             complement(33..749)
                     /locus_tag="MB022_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBT4467404.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(976..1518)
                     /locus_tag="MB022_002431"
     CDS             complement(976..1518)
                     /locus_tag="MB022_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4467405.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(1523..1750)
                     /locus_tag="MB022_002432"
     CDS             complement(1523..1750)
                     /locus_tag="MB022_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4467406.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1829..3709)
                     /locus_tag="MB022_002433"
     CDS             complement(1829..3709)
                     /locus_tag="MB022_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4467407.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3898..4512)
                     /locus_tag="MB022_002434"
     CDS             complement(3898..4512)
                     /locus_tag="MB022_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467408.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4654..5214
                     /locus_tag="MB022_002435"
     CDS             4654..5214
                     /locus_tag="MB022_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4467409.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(5519..5833)
                     /locus_tag="MB022_002436"
                     /pseudo
     CDS             complement(5519..5833)
                     /locus_tag="MB022_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         1761 a         1230 c          954 g         2096 t
ORIGIN      
        1 aaaagacatg tcacaacccc ttgtttatct cattaatttg cttgtccata aacgatttga
       61 ttaaagccat ttttcaattc attattcgct gtctcgttga agtaaagata attatcggga
      121 ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa cccgaataga
      181 tagacgtgct ttttcagata tggttctgtc tctgcatcgg aatagacggc tgtgatttga
      241 tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac tccaccaata
      301 gccatcttcc actccccgtt caaaatagct tgagggactt taagaccata taagtccaca
      361 ctcatttgat tcgtataact tttgtattgt tgtcccattg tttggcccca tttggtcata
      421 aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcaac ttgttcgctg
      481 ctttctgaag aggaagatga agcctctaca gaagaacttg aagtagatgt ttgagttgtt
      541 tcagtcgttt tggaagactc tgacgaagta gaggtatctg ctgtttctgt acttgctgca
      601 gttgacttgc tctctgtgct agttgttgta gtagttgcaa ctgttgcttt cgctgtttct
      661 tttgttgatt cttttttcga ttcattcccg ttggtacaac cagctaacaa aaaagctaga
      721 ccaattgtta ctaaaatcct ttttttcaat taaaaaactc ctttcgatta ctcccacttc
      781 tttattctat caaataagca atgtgatttt attgattttt cgtattttct catcaatctt
      841 tcaaatcttc taaaaactga gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg
      901 cttcttttat tttaatcttc gtgattttcc aatttacctc acgctttata tttgtatttg
      961 aagaaacata gacttttagt catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt
     1021 tccttggagt ctttgtactt ggtagatgat ttctttattg aaacttaaaa gttcaacaag
     1081 caattgttgc aagccatatt tttcttcttt ttcagctaat ttgattgccc gtgtgataaa
     1141 gacattttgg aaatcaaaat cttttgcaaa gttttccaac attgttgagg ctggtaaata
     1201 tttgttttga ccatgttcgt aaatttttcc ataacgcatg acttcttcta aagtagacgg
     1261 aacgatttca ttttcaatca ctaactcatg agagatttta ttgaaccatc tcgcttcttg
     1321 atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa
     1381 ttgtgcttgg atcaatttca tttcttctaa tttaaaattt gctaatacat gccccaccat
     1441 ggcgccagca gttggtttat gatggtcgat atctgcttgt tttgtttctt gaacaaattt
     1501 ttcttccact atattcattg atttacgcct cttttacttt tgctgattgg acttcataac
     1561 ctaatttttc cacaactgca gctaattgtt cagggtggac ttcatctgga tcaaagtttg
     1621 ctttgatttt gctcgcatta aacaaaactt tcgtattgct taccccaggc tgttgagaaa
     1681 ccgcatgttc gattttttgc atacagcttg gacaagctag tgtatctaat tgcataataa
     1741 tttttttcat cgtagttctt ccttcctttt tcttgatagt cttattctag attacagctt
     1801 ccataaaaaa attgattctt atcaagtttt acgatttttc ttctttgctt tcgtgtccaa
     1861 aatacaataa acgcatcgcg ttaaagataa cgatcaagat actagcttcg tgaacgaaca
     1921 tcccactagc catgtaaata aacccggcaa ataagccgtt gagtaaaaat aagacgacac
     1981 caatggcaat cactacattt tcacgggtat ttaaaacagt tttcttagcc aagcggtagg
     2041 catgaaccaa cgattcaaaa ttagattgca tcaagacaac gtcactcgtt tcgatagcaa
     2101 catccgttcc tgatcccata gcaatcccga tatcagccgc agccaaagag ggagaatcat
     2161 tgattccatc tccaataaaa gcaacgcgca atccttgttc ttggtatttt ttcacaaatt
     2221 gaaccttttc gtctggtaat aattcagcat ggacttcatc aatgcctaac atatcagcta
     2281 cataatcagc agtcatctga ttatcacctg tcagcatcac taactgtttt gctcctgctt
     2341 gtttcaactt agctaattgt tcagcgactt ctggtcgaat cacatctgac acacctatga
     2401 tttgagtgat ttttttgtcg attgctacaa taatgaccga gctgcctgtt tgttgtaatt
     2461 gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt gtgagcggca atcaatttgg
     2521 tgttgcccgc acaaactgta tgaccatcaa tctcagctgt gattccttgt cccttgattg
     2581 tctgattatc tttgactgct aattgttgaa aatcaatgtc tttctcctga gcataatgga
     2641 tcacggcgcg ccccagagga tgatcggata acgtttctaa tcgagccact aaagctaagc
     2701 tgtccccttc atcctctgta tagttcttga tagtggatac agcagtagtt cctttggtca
     2761 atgtaccagt tttatcaaag acaaaagtat cgattttgga aaaagtatcc attgcttcac
     2821 cgcctttgac taagatgccc cgtttcgctc cattaccgat tcctgctaca ttcgaaacag
     2881 gcgcaccaat gactaaagcg ccaggacaac caagaaccaa aacagtgatt gctaaacgaa
     2941 aatcacgact aatcagacca acaaccaacg caataagtaa aacggttgga gtataatact
     3001 gagaaaatcg atcaatgaat ttttccgcat gggatttggt atcttgtgct tcttctacta
     3061 attcgataat tttggcaaag gtcgtatcat ctccgacctt agttgcttca atcgtcacgt
     3121 agccatctgc taaaatcgtt ccagagaaga cttcatctcc actgattttg acgacttgtc
     3181 gtgattcacc agtcactgcg gcttcatccg catacccttt cccgtcaatg actcgtccgt
     3241 ccacaggaat agctccacca gtttttacta agacgatatc gccttcttcc acgtcatcga
     3301 tttccacttc ttcagtcgtt ccatcttctt ggacaacact agcagtagat ggggccatct
     3361 cggtcaaagc cttgattgac tcccgcgttt tggtcaatgt tttttgttcc agatagctac
     3421 caaacaaaaa caagaatgta acgatagctg actccttgta ttctccgatc ataaaggcac
     3481 caagcacagc aatagtaacg agtaaatcaa ttgagataac tttgactttc actgcctgat
     3541 acgcatggat cataataggt atgaccgcaa taatagaagc tatggctaga gcaatgttat
     3601 agaacaagac aaaaccagta aattttccga taaaaccaaa aataatcaac acagctgata
     3661 aggcggtgat atgatttcga tgctcttgga tccacttttg taatttcatc tttctccctc
     3721 ttttcctttc attcttacga gactaagtat agatacttat ccatcataaa aaattgacca
     3781 ctatcaagtt tagtcaaaaa ggcggttttc ttttggtaga atacactaca aaaaatagtt
     3841 agcaagaaga ctcactaaaa gtgatcttgc caactattgt cgtttctatc gaaattttca
     3901 ttgaatgatt ttcctgtaca gcatacatcg gagatctatt tctttatcta agatttttat
     3961 cttgctaatt ttttccttta gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca
     4021 gccactatct gtaaaaagga atatttcttt tcctttctct cttttttcaa attctgttag
     4081 caacttggta ccgatgcctt ttgttttcaa atcaggattt gcagctaaaa aaatgatctc
     4141 tccatctggc gaatgcttct ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta
     4201 agctccttta ctttctttag caaatatatt ttgtaaaaaa tcgaatatct taacatatat
     4261 ttgctgtcca aaagaacgat gtttcttttt ttctcctttt atctccgcca gcaaaacacc
     4321 tgccaactcg tcctctgcat acaaagcaag aatttgagtt gctcgattca actccatata
     4381 caaaaaatat cttccgtaaa gattcagcag aagttcattt tccatatatc tctcaaagtt
     4441 cattccttca acagcaaaat agatagcttt tttgtaatct ttcttttcta agggtttaaa
     4501 agttagtttc atcatttcgc ctcctcaact tcaatgattg tgttatactt acattataat
     4561 agacacagtg tctactatct ataatcaatc atctataagt ttgtcgatta agcgacagct
     4621 ttgctttgat atgtcggata aggagatagg taaatgaaaa agacagatag acgcgtcaaa
     4681 aaaacagaaa aagctttggc tgaaacacta tccacattat tagtaaataa aaagattcaa
     4741 gcaatcacta tacgcgaact aacagaaacg gcagatgtac atcgttcgac tttttataca
     4801 cattataagg atatctatga tctatatgat caattagaaa gcaacttttt tcgggatcta
     4861 aatgaaattc tctcttatga tccagcccat agctatgagg aactatacac tcgcttgatt
     4921 gactatgttt acgcaaatac ttttttatat cgcttattta tcctcaacca tggtgatatt
     4981 tcgtttcaga agaaagttaa acaagttatt gaagaaaact atctgaacat atggctatat
     5041 gaagacaaga aaacgattat cacagaagaa atgagattct ttactacgta ccacgtccaa
     5101 ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt acaagtactc caaaaaagaa
     5161 ttattagaac tcttacagaa actgaatgat cacatagaga aaatcatgcc ttgaataaaa
     5221 acatactaag cttagttaag aagaaccgta accttctcgt ataacaactg aatcttattt
     5281 agccaaattg tttactgaag attttttgag taaaatttct ataattccta cttattgaaa
     5341 agtagtttgt agtgctgctt ctccctctct aagtgatata tcttgttgta ccaatcaaat
     5401 tcacctcatc attaataaat tacttttagg ctaatttatt acaacttata ggttgaagct
     5461 gtaaccaatt tatagtcata ttatattttt atcatccagt ttattgaatt cttctcgttt
     5521 acctcaattc attttcaagg ggaatcagct catcatctaa cattttacgc catgctcgct
     5581 tcatttcttg tgggtattta aatggtttcg cttctggatg tttggcttta tatccttcgt
     5641 aatccttact gttgataatt gctttcatat taggaattgg atcttcacct ggcatatatt
     5701 gcagattaga agcatgtaat tttttctcag aaacaaacca ctcattggaa aagacatgaa
     5761 tcaaatcatc acttacttgt gtaaaatagc gacgtttaat gacaaagata tcttcattgc
     5821 tatctagtgt accttcattt ctttgtgctt aaattcaaag ataattttct caataaagat
     5881 ttttgattcc agtaagattc ctttactcct tttacaacat agcgttgtcc tttgttttga
     5941 aacatcttct tcccctcttt gccattaaaa atagcccaat tctctataaa gaagattaag
     6001 cttcattatt ataatataaa gtttaaattt ggtttattac t
//
LOCUS       DAGCXG010000117         6019 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGCXG010000117 DAGCXG010000000
VERSION     DAGCXG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.122"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            35..1336
                     /locus_tag="MB022_002437"
     CDS             35..1336
                     /locus_tag="MB022_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4467410.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1420..2367)
                     /locus_tag="MB022_002438"
     CDS             complement(1420..2367)
                     /locus_tag="MB022_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBT4467411.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2545..3348)
                     /locus_tag="MB022_002439"
     CDS             complement(2545..3348)
                     /locus_tag="MB022_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBT4467412.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            3494..4408
                     /locus_tag="MB022_002440"
     CDS             3494..4408
                     /locus_tag="MB022_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4467413.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            4456..4926
                     /locus_tag="MB022_002441"
     CDS             4456..4926
                     /locus_tag="MB022_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBT4467414.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            5011..5619
                     /locus_tag="MB022_002442"
     CDS             5011..5619
                     /locus_tag="MB022_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBT4467415.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            5753..5953
                     /locus_tag="MB022_002443"
     CDS             5753..5953
                     /locus_tag="MB022_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4467416.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1967 a         1001 c         1168 g         1883 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat ctcactctat
     1381 cagtcctatt tgacaaagag ccaatattgt tttcagctat taatatcctg cagacaattt
     1441 tacttgattg gtttgaagct catgggtggc aaagtagctt ttcagattgg ttaaaaaaat
     1501 agccattaat ttcttttgaa aatcaggtgt caaacctgca atgtgggaag taatcagtac
     1561 attgtccatt ttccaaagag gatgatcttg tggaagaggc tcttcctcaa aaacgtctaa
     1621 tgctgcaaag gaaagttgct ggttatttaa agcctgcacc agatcttgtg tcttaacaga
     1681 agcaccacgg ccaacattga tgaatacaga ttttggatca aagtgttcaa acatatgctg
     1741 atcaaagagc ccagttgttt cctccgttag aggtaagata ttgactacga aatctacttc
     1801 aggaagaact tcctttagtt gatttaaaga aaaagtcttt tccattcctt ctgctggatg
     1861 accggatgta ttgattccat agcagttcat gtctaatgct ttgcattttt ttgcaatcat
     1921 ttttccaata tggccagttc caatgattag tatttttttc ccttggatag attggtaatg
     1981 gaccgatgaa cctaaccatt tcatgtcact ctgtgccttt tgtgcttgaa aaagaccacg
     2041 agaataccca aggatgattc ctaagacatg ctccgtaatc gattcactgt ggatgccact
     2101 accatttgaa agtaaaatat gtttctttga aaaagtctca aggggtagct gatcaacacc
     2161 agctgagata gattggaccc atttcaaggt agaagaggct aatagttttt cttggtagtc
     2221 tgagttccag cccaaactga tctctacatt ttgtagatcc tctggttgta attctgtttt
     2281 aaagatataa ccaggcgcta tagtatggat ttcttcgata aattcatcac gaaatggacg
     2341 atctgcataa atgattcttt ctgacatagt taatttcccg cctttcttta ctctaagtgt
     2401 agcgcataca agaagaactt tgaagcgcct tgcttgtttt ttgagaaaga aatatttttc
     2461 aaataaaaaa ggggctgtga caaaaaaatg tcacaacctc taaaagggtc tattgaagta
     2521 gaaataacga gttgtcttta tttcttaatt gatcgatatt tttgcccatc ctttgacagc
     2581 ttgagcaaaa ataaagattc ctgcaaaagc aatgaaggcg ctagtgtcaa aagaaggaag
     2641 aaagaccatc aagattgttg caattatagt aggcaatgaa attgaataga tcaatgtttt
     2701 taaacaatca agaaaagtag cttttgttag tcgaaagcgt gcatagagaa aagcagcaat
     2761 gttggtaaat agcaaagtga tcagcagatt cagaaaacta ggataaatag aaaatataaa
     2821 tgtcaggact tttatccaaa gtggtataga tgcttcactt aaaaaagaac ggagctgctt
     2881 acctgttaag ttttttaaag attcattggt gtagggaagt tctaattggt tattgttcaa
     2941 caaagcagaa gtcgtgcctg tattaggaag agctatgata agtttgtttt ttaacatccc
     3001 gacactaaca aaattcccca tcaaatcagt agagatgtcc tgttctgttc gttttccctc
     3061 tggatcaaat gtgaagataa tggagtttgt ttggtaaatg aatccttctt tttcttcggt
     3121 ttgaagctgc ccgttttgga ttgtaaaatc aggaatgcga gtagcaatct tttgcccatc
     3181 caattgaatg ttgtttaaga cttgaaaaac ttgaaaagag attggcaaag ccataattat
     3241 actcaaaact aaaagataga caatggcttt ccaaaatggg acatttctcg cctcctttag
     3301 gtctttgaat cgtataaagg aactgataat taattgtttt gttgtcataa gtactcctca
     3361 tctagtagtg tcatcacttg caattctagc gaagtatcaa gtgaaataca agtgctttaa
     3421 ggaaacagct gaatgattgg tgaaatatca aaaaaatgtt attcttgtct aaagactatc
     3481 caatataaat aaaatggagg aaggaatagt gcctacacag ccacagagat catcaaaaaa
     3541 actatttgga gtagtggttt tgaatgcggt gatcacttta tcagaattta ttggagggat
     3601 tatgtcaggg agtttgtctc ttgtttcaga tgctttccat aaccttagtg atacattggc
     3661 aattattttt tcttacgggg cacaaaaaat ggcccgaaaa gaagcaaata aaaaacgtac
     3721 gtatggctac cagcgtttag aaatattgag tgctttcatc aactcattca tattgatcat
     3781 attatccttg tttttaaccg ttgaagcatt caagcggttc acttcaccag aaaagataaa
     3841 tagtcatttg atgctaacag tagcagttat tggattgctc gcgaatttgt tctcagtatt
     3901 attattacga caagaagcag atgaaagttt gaatataaaa tctagttatt tgcatttgtt
     3961 aagtgataca ctgtcttcgg taagtgtgat tatcggagca attttgatcc gttttttcga
     4021 tatctactgg atcgatccag ttattacact gatcatttcg atatatatat tgatagaagc
     4081 gataatggtg atcaaaaaag ctgccgctat tttgatacaa agtgcacctg ctatcgacta
     4141 tgaaaaaatg gaacaagaaa ttgaagcaat cgatggggta aaggatgttc atcatgtcca
     4201 tatttggcag tatagcgaaa agataattat tttcgacgga catattgatt ttgaagatca
     4261 gctactaagc gagattgaaa agatatatcc tcgtataatt tctttattaa aattaaaata
     4321 tggtattaca catgtgacga tccaagcaga gacaaaggca aaggatcaaa aaaaattaat
     4381 ttttttaaat aaagatgaaa gttattgaaa tatagagtat gataaataag aatttttaat
     4441 aaggtaggtt ggctcatgaa gcagtatcga cagttaaaag gatatttaga agcaaaagga
     4501 tattgggaga tactagtcta tctctttttt ggtggtttgg ctacgattgt aaattttgtt
     4561 agttttgctc tcgcacgcca atattttgat ttaagtatgg ccttatccaa ttctatttcg
     4621 tggttttgtt ctgtattgtt tgcttttgta acgaacaaac tttgggtatt ccattcaaaa
     4681 tcaccgaatt ttacacatgc acttattgaa tgcggtaagt tcttcttcta ccgtatcctt
     4741 tcttacggat tggacatggg ggcaatggta ttgttgatca atgttatgaa cagcaatgag
     4801 tatgtggcga aaatcatcac tcaaattatt gttatccttg cgaactatat tttcagcaag
     4861 ttgtttattt ttaaagaaac tgaggttttt gaagaagaag taggtcaaag tgaaaaagac
     4921 gactaggcat aacaattgaa gaatttcctt ctctttgtta ctctttgtaa tgtagacatt
     4981 attaagaaaa cattgaggag gaattttttt atgacttaca cattaccaga tctaccttat
     5041 gcatatgacg cattggaacc ttatattgac gaagaaacga tgcatctgca tcatgataag
     5101 catcacaata cttatgtgac gaatttaaat tcagcaattg agaaataccc agaattaggc
     5161 gaaaaaacaa tagaagaatt attatctgat atggacgcta ttccaacaga tatcaagaca
     5221 gctgtacgta acaatggtgg cggacatgct aaccattcat ttttctggga aatcatggca
     5281 ccaaatgctg gtggcgaacc tacaggagaa ataaaagaag cgattaatga agcttttggt
     5341 gatttttctt cttttaaaga agaattcaaa aaagcagccg ctggacgatt tggttctgga
     5401 tgggcttggc ttgtaatgga aaatggaaaa ttagctatta cctctactgc aaatcaagat
     5461 tctccattga tggaaggaaa gacaccaatt ctaggtttgg acgtttggga acatgcttat
     5521 tatttgaaat ataaaaatgt ccgaccagat tatattgcag cattctggaa tgttattaat
     5581 tgggatgaag taaacaaacg tttcgaagca gcaaaataac catttcctcc gtcatatcag
     5641 tcttaaaaat aatttggaaa ataaaagagc gaaagcttgc tagcgttttc taaattgggt
     5701 ataatgttct tgcaaggtta cttgcaagaa cttttttgga ggatatacat ttatggaaca
     5761 cggaacggtt aaatggttca acaatgaaaa aggatttggg tttattacag tagacggcgg
     5821 agatgacgta tttgttcatt tctcagcaat tcaaggagac ggcttcaaga gcctagaaga
     5881 aggtcaagaa gtagaattca cgattgttga aggtgcacgc ggacctcaag ctgctgaagt
     5941 gactaaatta taataatttg ttgaataaag acacgacttc ttagagagtc gtgtcttttt
     6001 gtctatttat gaagttttt
//
LOCUS       DAGCXG010000118         5947 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGCXG010000118 DAGCXG010000000
VERSION     DAGCXG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.123"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            43..2196
                     /locus_tag="MB022_002444"
     CDS             43..2196
                     /locus_tag="MB022_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBT4467417.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2165..3580
                     /locus_tag="MB022_002445"
     CDS             2165..3580
                     /locus_tag="MB022_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBT4467418.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3613..4647
                     /gene="sppA"
                     /locus_tag="MB022_002446"
     CDS             3613..4647
                     /gene="sppA"
                     /locus_tag="MB022_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBT4467419.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4649..5170
                     /locus_tag="MB022_002447"
     CDS             4649..5170
                     /locus_tag="MB022_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4467420.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5167..5553
                     /locus_tag="MB022_002448"
     CDS             5167..5553
                     /locus_tag="MB022_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBT4467421.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2122 a          805 c         1115 g         1905 t
ORIGIN      
        1 atttttctat aaaaattttt tagaaggagt tgtgttagat aagtgataaa atcatttcct
       61 tttgtgcagc aacaagatga aaaagattgt ggtgttgctt gcctttcgat gattttaaaa
      121 tattttaaaa ctgatatccc gattcataaa ttacgggacc tatctgggac tgatttagag
      181 ggtacttcag catttggatt gaaaaagaca tttgaaaaat tgtcttttga ttgtatggct
      241 attcaagcag atagtgagat ctggaaagaa agagatctta tcttaccatt aattgcacat
      301 atattggttg ataacagcta tatgcactac gttgtaattt ataaaacgaa gggaaacgta
      361 ctatggattg cagatcccgc taatggaaaa aatagaaaaa cgattgaagc gtttcaaaaa
      421 gaatggacag gtattctttt attacctatg cctagagaaa cttatattcc tattaaagaa
      481 aaagtagctg gattaagttc ttttttgcct ataatttgga aacaaaaagg attagtattc
      541 cacatcgttt tagcatcgat tttcattacg ttttttggca taggaagctc atattatttt
      601 caaggtattc ttgatttttt catccctaat caagcacgtt caactttaaa tataatttcc
      661 attggcttga tcgctgtcta tttatttcgg gtattgtttg aatatagtcg tagttatctt
      721 cttattattt taggacagcg aatgagtatg gcaattatgc ttcagtattt caaacatgtg
      781 ttaactttac caatgaattt ttttgctacg agaaaatcag gggaaattat ttctcgtttt
      841 cttgatgcca gtaagattat tgatgcttta gcaagtgcta cgttatcaat atttttagat
      901 attggtatgg tgttgcttgt tggggttacg ttagctattc agaatagtac gttgttttta
      961 atcactctat gctctttgcc tttttatgta gttgcaatat tcgctttcgt gaaaagctat
     1021 gaaaaatcga acagggaaga aatggcagcc ggtgcagctg taaattctag tatcatagaa
     1081 agtcttaaag gaatagaaac aattaaagca tatagcggtg aagagaaagt atataaccgt
     1141 atagaccatg aatttgttaa attaatgaaa aaatcatttc gtacggttac gcttgataat
     1201 attcaacaag gaattaaaca tttgattcaa ttaattagca gtgcattaat tttatggcta
     1261 ggttcttatt ttgttatgga taatagaatt agtctaggtc aacttatcac atacaatgct
     1321 ttactagtat ttttgacaga tcctttacaa aacattatta atttacaagt gaaaatgcaa
     1381 acggctcaag ttgcaaataa tcgtttaaat gaaattttcg cgattgattc agaaaaatcg
     1441 aaccagtctt ttgaatatat gctttctgaa caattatttc aacaagatat cactgtaaaa
     1501 aacgtttcat tctcgtatag catgaaggcc ccaacgttga acgaaatttc atgtacaatt
     1561 ccttcgcaca gtaaaacggc tatagttgga gttagtggtt ctggaaaatc aactcttgca
     1621 aagttatttg ttcgttttta tgaagcatca gaaggatcga tccattatgg aaacattaat
     1681 cacttagaca ttccttttca gatattacgc gatcgagtga catatttgcc tcaagagtca
     1741 tttttcttta gtggaacaat cattgagaat cttatattcg gtttagatag cccaccaagt
     1801 ttcgaacaaa taatagaagt ttgtgagata gtccaactaa atgaatttat taatcaacag
     1861 ccattacgtt acgagacatt gttggaagaa ggtggaaata atctttcagg agggcaacga
     1921 caacgattag ccttagcacg ggctttatta aaaaatccgg acatattaat tttagatgaa
     1981 gcaactagtg gattagatac cttgttagaa catgcaatta tgaattatct cctcaattta
     2041 aaagaaaaaa cgattatttt tattgcgcat catttaccaa tagcaaaagc atgtgatcaa
     2101 attttagttc tccatgaagg gcatttggta gaaaaaggaa cgcacgatga acttcgtttt
     2161 gccgatggta tctatcaaaa attatgggaa atctaggaga gggaaacaat ttatgcagaa
     2221 taataattgg ttcgaacatt ctagtatcta tactaaacaa catcatagct tctaccgttg
     2281 ggtgttatac cctgtaattc tttttttatg tattattgga ttgtttttaa cctttgctaa
     2341 aaaagaggtt gttattcgga caacggctca attgaacccc gaaaaaatag agaagttaca
     2401 agtgccatta gaagctaaaa taatggaaaa cttcctaaaa gaaaatcaat ttgttcataa
     2461 ggacgatgtg ttggttagat tggattgttc tttaatagag aatgaaaagg ctcagataga
     2521 acaagaaaac caacgtatta ctcaacaaat aaagatggct cagctattta ttgaaagtat
     2581 aagtaaagga aaaaatttgt tttcaacgga tgacagtttt ggctacagta atcaattaaa
     2641 gagcatgttg tcagaaaaag aatcactccg ctacgctttg aagcaaagtg aattaaatga
     2701 tcaaaagcaa ttagaagtat acgaaaagac aaaaagacaa ctagaaaaac aaattgagag
     2761 ttcagatagt aaattacaag aatggcaaca agtacaggta gcttggagta ataatcaatc
     2821 attaaaagat ttttcaaaag aaatgatggc aaactatgag aattggcaag aacaactaaa
     2881 taatgtttct gatgatcaaa aaaatcaagt gaaactgaca atttcagcaa gcataaatga
     2941 acaaattgag caactaaaaa aagaagtaga acagtatcag tcagaaaaag ctaaattagt
     3001 taaaccaact acttctgaga atgacagaat tagtcaaacg gaaaaaggaa agcaagagct
     3061 agaacaagca ataacaactg taaaacaaac agttacagag ctaaatgaaa aacaagagaa
     3121 gaaccagtca gttatcaaaa gtttggacga tcaactttca aaaggaatac taaaagctcc
     3181 tgtatctgga acggttcatt taaatgaaga aacaaaggga caagcagaac tagccaaagg
     3241 aacagtgctt gcagaaattt atccgatcca cgaaaaatca aaaatgaaat ttacagcact
     3301 tttacctgcg aatgaaagta cctatattaa agaagggatg aaggttcatt ttaaattgga
     3361 tcaaaaaggt ggggctccga ttacgatcga cggaacttta gatgaaattt cagaaaattc
     3421 tactgccact gagaaaggtg ttttttatgc agtgaaagga acgttaaaaa caactcaatc
     3481 gtttccgttt cgttatggat taactggaga gttgtcttta attgtaggag aaaaaacata
     3541 ttggcaacaa attgtcgatg tgtttctaaa taaaaaataa ttatttttac taagaatata
     3601 ggaggagttt atatgaataa aagaagatgg attgctgtgg gagtagcgtg cggattattg
     3661 ttactatcca ttatcgtcgc gctaattcca ggaaaagata aagaggaagc atcaaataca
     3721 accctaacag gaataaataa gattttttac ggatctaacg aaatcacaga agaaacgtta
     3781 gaagaaggcg catcaaataa aaagattgtc aaactatccg tcaatggagt tattgcagat
     3841 acaggtgaaa gcaatctttt tagtagggaa caatataacc atcagaattt tttaacacag
     3901 ctaaagaaaa ttcaagaaga caaagcggtt aagggagtgt tattagaagt aaattcacct
     3961 gggggaggaa tatatgaaag tgctgagatt gctaaagaaa tggcaaaaat taaaaagcta
     4021 gatattccta tctatactgc atttaaaaat acagcggcaa gtggagggta ctacatttct
     4081 gctggttcag ataaaatatt tgctacggaa gaaacaacaa ctggatcaat tggtgtcatt
     4141 atttctggtt taaattattc tggcttatta gaaaaacttg gcgtaactga tgcgacgtat
     4201 aaaagtggtg cattaaaaga tatgatgtca cctcaacaca aaccttcaga ggaagagaat
     4261 aaagttatac aggaatttgt gatgagtgct tatgatcgtt ttgtcaatgt tgtagcaaaa
     4321 ggaagaaata tggatactaa tgcagtgaaa gaacttgcgg atggaagaat ttatgatggt
     4381 aaccaagcag tagaaaatgg gttggtggat caaattggtt actctgaaga tgcattagat
     4441 tccctaaaaa aagagaagaa attaactgat gcgacggtta tagaatataa aaatgacaca
     4501 actggctttg cttcttcttg gttaggtaat aagattgctg aatggcaagg gctaaaggca
     4561 actacatcag atcgtgtaat ttcagttttt gaaaaacttg gattttctga tactccaaaa
     4621 ccaatgtatt actatggagg gctataaaat gaataagaag agtggcattg aaactttgcc
     4681 taattatgta tttgcgggat tttggttacg attttttgct tttcttattg atttaatttg
     4741 tattagcatc atcacccatg ctactatagg tctcttttac aaaatcatgg gactggaatc
     4801 tgacaaatcc attattactt tttatggtat agcgtcatta gctatttatt taacctattt
     4861 tgtacttttg actaagctga attcaggaca aacaattggg aaaatgattt ttgggataag
     4921 agtagtgagc tttgaggaaa aagagctaag ttggcaaact atctttattc gtgagtgtat
     4981 atgtaggttt atattaaaaa attttgtatt tcaaattggg tatttggtgg ctgctttcac
     5041 gcccaagaaa caacatattg gggattattt ttctaacacg tcagtggttg ttattcgttc
     5101 attagaagct attaactatg gtcaaaaaat ttctaattct aaaacactta atctggagag
     5161 aattaaatga ataagagaaa aacagagttt attttgattt taattagtgg aataacaggg
     5221 attttatgta gtcttttagc attgatgggt ggtatgtttc tatcttctat tgattcaact
     5281 actttaaata ctacagaact aaccagtaag gatatagata tgttagtcaa tctaagcggg
     5341 aatgttgttt tatttggagg aattacccta gttttttcga taatgatttt cataataggt
     5401 tttttagtaa gaaaaaatca aaaaacagtg gtctatggta ttagtttact cattttatct
     5461 attattccat tttttctttt aacattattg tgggtaattc ctggagtttt aggtattatt
     5521 gcgggattga tgcttctaat aagaaaatct taattaggca atgtgtagac aacgtgaaac
     5581 gattaatcaa ttaaaagcta aatacgtttt gagaaatgat aacttgtagt cgatggaaga
     5641 catgtagata aaaaaactac gtgtctttta tttattctaa ttttttcgaa aaaaagacac
     5701 tctctgattg ttgtattaat ggaaaagggg aaagcagcaa gtaaatttag tttttctatt
     5761 aaatattctt cactttttaa ccttttatag tacttagatt taatcctata tgtttagaga
     5821 gttcattggc agcatgattt tgcaaagctt ctcgaattat tgttatatta gaaactttac
     5881 tgttcataat tatgcttctt ttttaactca aagtccaaag ggctacagtg gactttgagt
     5941 taaaaat
//
LOCUS       DAGCXG010000119         5830 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGCXG010000119 DAGCXG010000000
VERSION     DAGCXG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.124"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(56..514)
                     /locus_tag="MB022_002449"
     CDS             complement(56..514)
                     /locus_tag="MB022_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4467422.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(527..1858)
                     /locus_tag="MB022_002450"
     CDS             complement(527..1858)
                     /locus_tag="MB022_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBT4467423.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1869..3311)
                     /locus_tag="MB022_002451"
     CDS             complement(1869..3311)
                     /locus_tag="MB022_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4467424.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3298..4062)
                     /locus_tag="MB022_002452"
     CDS             complement(3298..4062)
                     /locus_tag="MB022_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBT4467425.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4166..5041)
                     /locus_tag="MB022_002453"
     CDS             complement(4166..5041)
                     /locus_tag="MB022_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4467426.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5296..5380)
                     /locus_tag="MB022_002454"
     tRNA            complement(5296..5380)
                     /locus_tag="MB022_002454"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5344..5346),aa:Leu,seq:caa)
     gene            complement(5398..5469)
                     /locus_tag="MB022_002455"
     tRNA            complement(5398..5469)
                     /locus_tag="MB022_002455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5435..5437),aa:Gln,seq:ttg)
     gene            complement(5479..5551)
                     /locus_tag="MB022_002456"
     tRNA            complement(5479..5551)
                     /locus_tag="MB022_002456"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5516..5518),aa:His,seq:gtg)
     gene            complement(5613..5697)
                     /locus_tag="MB022_002457"
     tRNA            complement(5613..5697)
                     /locus_tag="MB022_002457"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5661..5663),aa:Leu,seq:caa)
     gene            complement(5718..5788)
                     /locus_tag="MB022_002458"
     tRNA            complement(5718..5788)
                     /locus_tag="MB022_002458"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5754..5756),aa:Cys,seq:gca)
BASE COUNT         1769 a         1247 c          892 g         1922 t
ORIGIN      
        1 aaattttgta aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa
       61 attcaaaaca actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa
      121 aaatgcctgg agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca
      181 aatctataat agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct
      241 gaaacggact tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc
      301 cagattcact ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt
      361 taactggcgc atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt
      421 ttgaagaaaa gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag
      481 cttttagaat gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg
      541 gccaatattt tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat
      601 ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt
      661 aagcatctac tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat
      721 cactgattgg ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta
      781 tttcaacaac tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca
      841 taaagcgacg acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat
      901 gctccccaaa attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca
      961 tttctttggt atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt
     1021 cagtatgctc aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat
     1081 tacttggaaa atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat
     1141 ctcctatatt aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg
     1201 gcataatttc tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta
     1261 aaccataata cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt
     1321 cttcaattga aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac
     1381 gaacagcttc agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg
     1441 catagtcttg tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag
     1501 caaatcctcc gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt
     1561 cgtccttttc tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc
     1621 ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc
     1681 ttttcagcat aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat
     1741 ataaacgtat ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc
     1801 ctggtgtata cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct
     1861 tttcctttct attcttctaa taatttattg aattctgcca taacataagt cacttctaag
     1921 ccgatgataa tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc
     1981 ttgattcgct gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta
     2041 caactataga catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc
     2101 atatattcat aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg
     2161 ccaaactctg ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa
     2221 taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca
     2281 ttggtagcga ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca
     2341 attcgtatat tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc
     2401 acgtacaaca taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca
     2461 ataaatgaag ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta
     2521 taagctgctt tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa
     2581 aaaactgata aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct
     2641 ctgaaaactt ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc
     2701 aacacatggt gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca
     2761 cctaatgctc ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc
     2821 ggccagataa agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat
     2881 gggaattttt cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga
     2941 ttatacgtcc atgccgctac taaaccggaa ataattccac caaaaatccc catattcact
     3001 tctgggtcca taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt
     3061 gcaggtattc ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag
     3121 atatccaagt ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata
     3181 tcgtcagctg ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc
     3241 atcgctcttc caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta
     3301 aatatcctca tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc
     3361 tgaatgaatt aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa
     3421 caattcctga tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa
     3481 tatcatgagt aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc
     3541 atcagtttct cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc
     3601 ttttcgttca aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac
     3661 ctcctgattt tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat
     3721 ccccgcatga taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc
     3781 aatgttaaat ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt
     3841 atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag
     3901 cctaggcatt tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa
     3961 tgtagttgat gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg
     4021 gctatagcca aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt
     4081 tctactaaaa taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc
     4141 gcctatagat agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga
     4201 tgatttcatt ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta
     4261 gtgctgtaca atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta
     4321 ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc
     4381 gatgataagt cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca
     4441 aaatcgtggt cgtcatttct actattttta agctctttaa actattaggc tctcctagct
     4501 cagcattaat gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat
     4561 atcgatgatt gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga
     4621 atctcatttc tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag
     4681 caaaataaat atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc
     4741 tagctgttat cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg
     4801 agactatctc actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag
     4861 catcctcaac atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg
     4921 caattttttc tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca
     4981 catacaccgt taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca
     5041 tacatatttt tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt
     5101 tcttaggtcg tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca
     5161 cataataaaa gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat
     5221 tgatttggag gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg
     5281 ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg
     5341 attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg
     5401 gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc
     5461 tataccccaa gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
     5521 tccggtgcgt taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca
     5581 ataaaaaact aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg
     5641 ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag
     5701 ttgatttatt agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac
     5761 ctctgcctta ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa
     5821 taaattatag
//
LOCUS       DAGCXG010000120         5762 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.125, whole genome shotgun sequence.
ACCESSION   DAGCXG010000120 DAGCXG010000000
VERSION     DAGCXG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.125"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            82..1086
                     /locus_tag="MB022_002459"
     CDS             82..1086
                     /locus_tag="MB022_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467427.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1087..2031
                     /locus_tag="MB022_002460"
     CDS             1087..2031
                     /locus_tag="MB022_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4467428.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2031..2993
                     /locus_tag="MB022_002461"
     CDS             2031..2993
                     /locus_tag="MB022_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467429.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3020..3934
                     /locus_tag="MB022_002462"
     CDS             3020..3934
                     /locus_tag="MB022_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467430.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3960..5741
                     /locus_tag="MB022_002463"
     CDS             3960..5741
                     /locus_tag="MB022_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4467431.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
BASE COUNT         1864 a         1071 c         1260 g         1567 t
ORIGIN      
        1 tgttataaaa cgatttattc tgtgtaatgt ccttttttat taagaaaaac ttccatttag
       61 gaagcagaga ggggtattcg attgtccgat aaccaattat tagaagttca gaacctacat
      121 acaggattcc gactcaaaga tgactattac gatgcagtag atgacgtttc atttacttta
      181 gacaaaaacg agattttagc aatcgttggg gaatctgggt gcgggaaaag taccttggct
      241 actacgatta tgggattact tgatcctaat aacacaaaaa ttacaggtga gattttatat
      301 aacgatctga atttaatcga cctgaacgaa acgctctata ataaaatccg cggaaatgat
      361 atcggaatga ttttccaaga tccgttgtct gcattaaatc ctctcatgag aatcgaagac
      421 cagatcaaag aaggactttc ctatcacaca aagatgaatg cagaacagcg tcaagctcgt
      481 gcactagaat tgctagaaca agtagggatt cctaatcctg cgagagttgg acgccaatat
      541 ccccatgaac tatcaggcgg tatgagacaa cgtgtcatca tcgcaatcgc aatcgcctgc
      601 aaacctccaa tcgtcattgc agatgaaccg acgacagccc tagatgtaac catccaagca
      661 cagattcttg atttactgaa agaccttcag gaagaaacaa aaacagggat catcttgatc
      721 actcatgact taggagtcgt agcagaaatg gctgatcgag tggcagtgat gtatggcgga
      781 cagttcgttg aagtagcagg tgtaaaagaa ctgtttgatc atcctaagca tccttataca
      841 cgctctttac tgaactctat accacaagaa ggaaatcatg atgcagaact ccatgtgatc
      901 gaaggtgtcg ttccttctct gaaaaacatg cctcgtaaag gttgtcggtt tgcaccacgt
      961 attccatgga taccagcaag cgatcatgaa gaagagccta tccttcatga agtcgcaccg
     1021 aaccacttgg tacgttgctc ctgctataag catttccact ttagagacga aaaaggagag
     1081 gtgtagatga cagaattaat cacaatcaaa gacctgaaag ttcattaccc tattcgcagt
     1141 ggattcttca atcgagtaac ggatcatgta tatgctgtag atggtgtgga ttttattatc
     1201 gaaaaaggaa agacatatgg tttagtggga gagtctggct ctggaaaatc tactacggga
     1261 aaagccgttg tgggattaga aaaggtaaca gccggacaaa tcatgtatga aggaaaagat
     1321 gtaacgaagc gaagcaaccg caaaaaaatg ggttataaca aggatgtgca gatgatcttc
     1381 caagattcaa tgtccagttt gaatcctaaa aagcgagtgt tggatatcat tgctgaaccc
     1441 atccgaaatt ttgaacggtt aagcgatcaa gaagaaaaga aaaaggtaaa aggactgcta
     1501 gatatcgttg ggatgccgga agatgcgcta tataaatatc ctcatgaatt ttcaggcgga
     1561 caacgtcaac gtctaggagt agcccgtgct gttgctacca atccaaaact gatcgtagcc
     1621 gatgaacctg tatctgctct ggatctatct gttcaagcgc aagtattgaa tttcatgaaa
     1681 cgaatccagc aagaatacgg cctcagctat ttattcattt cacatgattt aggtgtagta
     1741 aaacacatgt gcgacaatat cgcaattatg tacaaaggtc gatttgtaga aatcggtaca
     1801 agagaagaca tttataatga cccgcgacat atctatacga aacgtctgct ttcagctatt
     1861 ccgcaaatcg atgtagaaaa tagggaaagc cacaaagaaa atcgacgaag agtagaacaa
     1921 gagtatatcc aaaaccaaaa agaatactat gatgcgactg gtcgagtcta cgatttgcgt
     1981 acgatcacaa ctacacacaa agtggcgttg aaagatggag gtgcaagcta atgtggaaaa
     2041 cgattctgcg acgtgtttta ttgatgatcc cgcaagttat tattttaagt gttctgatct
     2101 tcttattagc ccaagcgatg ccgggggatc catttaccgg tttgatcaat ccaaatcagg
     2161 atccggcagt gatcgaacag atgcgacaag cagctggctt gaatgaccca tggtatgtcc
     2221 agtacttccg ttggatcgga aatgctttgc aaggcgattt tggtcaaagt ttcttataca
     2281 aacgctctgt tgctgacttg atcggcgaac ggatcatcaa cactttatat ctatcgatct
     2341 tgacagttgt ccttacttat ttgattgctg tgcctctggg gatgcttgct ggccggtatc
     2401 aaaattcaat cttggataag attgtcgtaa tctacaactt ctttagtttt gctgttcctt
     2461 tatttatctt cggcttgatc atgctatttg tttttggtta tcgtttaggc tggttcccaa
     2521 caagtggatc acaaacacta ggtttgcaag gtggatttat cactcaatgg atagaccgtt
     2581 tgaaatatat tctgttacca tcaattaccc aagcattttt ggcgactgcc gtcacaatcc
     2641 aatatctgcg aagtgaagta atcgattcga aatcgctcga ttttgtcaga acagcccgtt
     2701 ccaaaggggt accgacgaat aaagtattca gtcgacatat tttccgaaat gcagcgttgc
     2761 cgatggcttc tcaaatgggg tatgaaatca cttcgttgat tgctggttct gtagttatcg
     2821 aaaagatttt tggttatccg ggagtcggaa aactatttat tgattccatc ggccaacggg
     2881 actataccgt gatcacatcc ttagtattga ttttaggaat cgcgacgcta gtggggacat
     2941 tactttcaga tatcatcatg agtatcgttg acccgcgtat tcggattcaa taaaaccaat
     3001 cagaaaggag aatggatcga tgagtgaaaa aaacaataca gcagttcaag aaagtattcc
     3061 tcctatgggg atacgaatga ttgctcgaga atttaaaaaa gataaaattg ctattttttc
     3121 attaggtttg ctagtggttt tattattagt gatctttatc ggtgcgatgc tgaccgatca
     3181 agacaaagtg atgtatgtta gtatcttcga caaatacgct gcaccaggaa cagtttcgac
     3241 acaaggacaa aagttcattc tgggagcaga tgaaggcgga cgtgatgtat taggacaatt
     3301 gatcataggt gctagaaact ctgttacgat cggatttgcg atcacgatcc ttacttcgat
     3361 catcggggtt ggattaggga tcatctctgg ctactacgga gggatcattg acaacatctt
     3421 gatgcggatc gttgatttta tcatgattct accgatcatg ctgatcatta tcgtcttcgt
     3481 atcgattatt tccagataca atgtctggtc ctttatattc atcatgagtg cattttattg
     3541 ggtagctaaa gcccgattat tccgaagcaa gacattatct gaagcaagaa gagattatgt
     3601 cagtgcctca aaaacaatgg gaaccaatga ttttaaaatc atgttccgcg aaatcatgcc
     3661 gaatctaagt tcattaatca ttactaattt gacgatgaac tttgcggcaa acatcggtat
     3721 cgagacaact ttgactttct taggtttcgg cttgccggca aatgtaccta gtttaggtac
     3781 attgatcggt tatgcaagta acggagaagt cttagtgaat cggcaatgga tctggttacc
     3841 cgcatcaatc cttattttag ttttgatgtt gagtatcaat tatgttggac aagcattcaa
     3901 gcgttctgca gatgcgcgcc aacggttagg ctagtataga agcgaagggg gaaataggaa
     3961 tgaagaaaac gatttttgga tttgcagcgc tgatgtctgc tgtcgttctt gcagcatgcg
     4021 ggggcggagg tacagatacc tctggaaaca actctggagg caacggcaat aacaaagagg
     4081 aattaacttt tccaattgca acaaaaaata atgatgatgc aatcgaagac ggcgaattag
     4141 aagctgcggt cgcaacggac agtcagttta aaggattgtt ccagtgggag ttttaccaag
     4201 attcatatga tgcagctttt atggctcctt ctcatgaacc gctatttgca aacgatgacg
     4261 atttcagaat cacagacgac ggagctgcaa cgcttgatct agatgaagat acgaaaaaag
     4321 cgacaatcaa agtaaaagac aacgtcaaat ggtcagatgg tgaagatgta acggcggacg
     4381 atgtgatttt cccttacgaa atcattggga ataaagatta cactggtatc cgttatgacg
     4441 acactttccg taacatcgta ggtatggaag aatatcatga tggaagtgcc gacacaatct
     4501 ccggaatcaa aaaaatagac gacaaaacag ttgaaatcga atataaagaa gtaaacccaa
     4561 gcatgcttca atcgggtggt ggtatctggt catatgcagc accaaaacat acgttagaag
     4621 gtgtagcaat caaagatatg gaatcaagcg atcaagtacg taaaaatcca gtgacttttg
     4681 gcccttatta catgagtaat atcgttgcag gagaatcagt agaatacttg ccaaacgagc
     4741 actactggaa tggtacgcct aaattgaaaa agatcacgat gaaatctgta ccgatggcaa
     4801 gtgcaactga agcattgaac tctaaattat atgatatgat tttccaaatg ccaacagata
     4861 cttacgatac ctataaagaa attgctggct atacaaactt aggccgtgaa caaacttctt
     4921 atacttatct aggattcaag ttaggtaaat ggaatgcaga tacaaagagt gtggaataca
     4981 atccagattc aaaaatggct gataaatcat tacgtcaagc aatgggctat gcattagata
     5041 ataatgcagt aggtgaacgt ttctatgcag gtttgagaag caatgcgact tcattgattc
     5101 ctccagtatt ccagtcatac catgatgatg agatgaaagg attcacacaa gatctagaca
     5161 aagcgaaaaa actattagat gatgctggct acaaagatgt ggatgacgat ggtttaagag
     5221 aagacaaaga tggaaataaa ctaacaatca actttgcttc gatgtccggt ggggaaacag
     5281 cgcaaccatt ggcagattac tatatccaac aatggaaaga aattggtttg gatgtccaat
     5341 acacaacagg tcgtctgatc gaattcaatt ctttctatga taaattagaa aacgatgatc
     5401 ctgaaattga tgtttaccaa gctgcttgga atacaggaac tgatccaaat ccaagtggac
     5461 tatggggacc aaatgctgcg ttcaactaca ctcgttatga atcagaagaa aatacaaaat
     5521 tgatcaacga tatcagttca agcaaagcat ttgatgaaga gtaccaaaaa gaagcatatc
     5581 aaaaatggca acaatatgca tttgaagaag catttgcgat tccaacgttg ttccgtaatg
     5641 aagtattgcc agtcaacgac cgtgtcaaag actgggattg gtcttatgat gtaccaaacc
     5701 catgggcaaa tgtttctgtt acagcagatt cacgttcata atcactaaaa atgaccgaaa
     5761 aa
//
LOCUS       DAGCXG010000121         5620 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGCXG010000121 DAGCXG010000000
VERSION     DAGCXG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.126"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..132)
                     /locus_tag="MB022_002464"
     CDS             complement(<1..132)
                     /locus_tag="MB022_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4467432.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            541..2913
                     /locus_tag="MB022_002465"
     CDS             541..2913
                     /locus_tag="MB022_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467433.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2974..4434
                     /locus_tag="MB022_002466"
     CDS             2974..4434
                     /locus_tag="MB022_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBT4467434.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4445..>5620
                     /locus_tag="MB022_002467"
     CDS             4445..>5620
                     /locus_tag="MB022_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4467435.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     "
BASE COUNT         2126 a          959 c         1192 g         1343 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaac
      541 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
      601 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaagaaag
      661 accaacggtc aaccaatgaa cgataagaga aatgggcatt cttggtttag gcaacaagag
      721 cgaatcgaaa ataaagtgca tagtctgaga gaagagatca agcaacaaga aaaacaggta
      781 gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      841 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      901 gaatcgactt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      961 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
     1021 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
     1081 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
     1141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgcacca
     1201 actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt caacgaaaat
     1261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga gttgatcgat
     1321 caacttaaaa cgttggataa ccaatacgct gcagaatcag gttattataa attctatttt
     1381 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
     1441 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     1501 gaacaaaaac aatcggtcaa aaatgaacct gttacgaaag aacaaatgca gtcgaaagaa
     1561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaggac
     1681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1741 ttgattgagg aacaatttcc aggtgcaacg gcatgtttag gtcgtgccgc gttgaaaaaa
     1801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1861 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1921 aaaaaacgaa tcgaaagtgg tcaaattgaa gtgatcaaaa aaccttacta cacgattgaa
     1981 aaagtgtttg atatcacaca aacacaattg aagcctgaag actatccgaa aatcttcccg
     2041 aaccgcgtct ttgattttca attggataaa acagggcaga cagaacttca agttgggatt
     2101 caagcggtag ccaaaaatat tggaattcaa attcgagata tgacagaaag tgaagtctat
     2161 caaagagaat taggacaagc acgtggggcg tacgttcgta acgaatttac tggcgaagaa
     2221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     2281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     2341 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     2401 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     2461 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     2521 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2581 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc tgttggttca
     2641 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2701 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     2761 agttatggaa aagagattca aacggcgtta ccttcagaag aattttcgct aggaaatcca
     2821 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
     2881 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctgaaaaa
     2941 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
     3001 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     3061 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     3121 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     3181 tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc cacagatttt
     3241 attctaaaga atgaagacaa aacagaacga gtagaaaatc ttcattttga gacagaaaag
     3301 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     3361 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     3421 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     3481 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
     3541 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3601 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
     3661 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3721 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3781 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3841 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
     3901 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3961 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
     4021 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
     4081 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
     4141 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg ctcgaatggt
     4201 acgtacgatt ttgatcgttt ttatcaagga aaaggggata ttaatgctca gattcttttc
     4261 tcttataaag cagaacaata ctacaaaggc tataaaaagc atgaacatga atttgtacct
     4321 gaagtgaaaa aggattggaa cgatcaattg aagcatgaga tccagcaaca agaaattaga
     4381 aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac aagaaaggga gtgagatcgt
     4441 cacgatgaaa actgtcattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     4501 cttttctaaa gccacccgaa aagacgggta ttttgagatc caagatcgat tgtttcctgg
     4561 tgaaactgtg attacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     4621 cgatgaaaat tggaaacaat ggtcattaga acatttacct attttcccta cacattatca
     4681 ctatcatgtt cctaaagata agaaaaagca attcaacgtg gtgaaacagc aacttcaatc
     4741 ggccgacacc attattattg cgacagatag cgatcgcgaa ggggaactga tcgcttggac
     4801 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     4861 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     4921 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     4981 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     5041 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     5101 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     5161 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     5221 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     5281 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     5341 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     5401 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     5461 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     5521 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     5581 tcctacaaag acggtcatga cggcagcggc ttttgaacaa
//
LOCUS       DAGCXG010000122         5370 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGCXG010000122 DAGCXG010000000
VERSION     DAGCXG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.127"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            646..888
                     /locus_tag="MB022_002468"
     CDS             646..888
                     /locus_tag="MB022_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBT4467436.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1239..1493
                     /locus_tag="MB022_002469"
     CDS             1239..1493
                     /locus_tag="MB022_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HBT4467437.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            1522..2781
                     /locus_tag="MB022_002470"
     CDS             1522..2781
                     /locus_tag="MB022_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBT4467438.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            2834..3730
                     /locus_tag="MB022_002471"
     CDS             2834..3730
                     /locus_tag="MB022_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4467439.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            3741..4610
                     /locus_tag="MB022_002472"
     CDS             3741..4610
                     /locus_tag="MB022_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4467440.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVFTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAAYGFSKLNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMASSVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            4620..5282
                     /locus_tag="MB022_002473"
     CDS             4620..5282
                     /locus_tag="MB022_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4467441.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
BASE COUNT         1709 a          817 c         1090 g         1754 t
ORIGIN      
        1 acatccatct ggaatttatc ttttttgctg cttttctcat aaaagtctgc atattcgtca
       61 ctgatatttt ttctaataaa ttcttccatc ataaaaatca cctcaaaacg attatacgaa
      121 cttacgttct tattgtaaag cgatatttga ggggcaaaaa gggggcaaaa atgcgtttac
      181 ctttgatata tctcagattg aacatagtgt ttattttatg tcggtattgt tgatttatag
      241 ctgttttgag ggtgttagat acataataga atatagtggt tttgtttacg tgcattacac
      301 ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat tgttatcaga ctgagaaaga
      361 acccttggta ttcaagggtt cttttttctt agcatgtaat ttgaacaccc tgatccagtt
      421 tttttcttta attattggta ggggagaata atggattaga ataaatctac ctataagtag
      481 tagtaaaaaa aagagcacta tgataatatc tttcttaaga gtaaccgctt taataaaaaa
      541 tccagaatat tctaacataa atggagtatt cttagaatca aaaggagaaa gaagatggtt
      601 tatacttata cggagcaaga aaaaatgaaa gtttgagaca cattcatgaa tgatggaaaa
      661 gtaaaacaag taccaagttc aacaaaaaag aaaaacatcc tattgaaaga agtcttgaaa
      721 cgctttgacc atggagtgac ttatacagaa acagaggtta attcgatact gctgaacgta
      781 ttttctagtg gcgattatgt cgaacaaaga cgttatctaa tcacatttgg ctttttcaaa
      841 agaagttcag acggtcgggc atatcagatg atgggaattg aaaattagaa gttttaattt
      901 atactcattg aaaaaagttc aagtatgcga tattggttct acaccgcata cttgaacttt
      961 ttttgtcatc tgtatctata atacaaagcc aagaacgtta ttcaaaatat ggatagctat
     1021 gataaaagct aatgagcgat tcgcagttaa ataagcaatc gtcagtaaaa tcgttccagg
     1081 agcgtacatg aaaaaatcaa ttggatgcgt tacatgaata aagataattc tcttttattt
     1141 gtacgaataa ataccaaaac gtacctccta aaattagatt caaagaagat agcaaagagg
     1201 aaaaagatag ttctgcagca gctatagcgg catgtggatt gctagaactt tccaaacaac
     1261 tgcctgtttc agatcctcgt catttcgatt acgaagagat agcaatcaaa atcttggaag
     1321 aattacagaa aaactataca acgaaacgag ttaacggatc aaacggtctg cttctccatg
     1381 ccgtttatga taaaaacagc ttaaaaggtg tagatgaatg tgtgatctgg ggcgattact
     1441 tttatgtaga aggaatcact cgtctagcta aaacatggta ctgttactgg tgatgtataa
     1501 gagaagagga ggattcatat aatgaagcga tggaaaaaaa tgatgattgg atcaatagtt
     1561 ttaggaatgt cactcgtatt tggtgcttgt ggtaatcaag aagacagtag tgaagaatca
     1621 gcaaatgaaa ttaccgtttc gacatggaat tatgaaacaa cacctgaatt caaagcgctc
     1681 tttgaagcat tcgaaaagaa aacgggaatc aaagtgaaag cagtagatat tgcttcagat
     1741 gattatgata cgaaactcac cacgatgctt tcttctggag acacgacaga tgtattgacg
     1801 atgaaaaatc ttttgtcgta ttcaaattat gctttacgag atcagttagt cgatcaaaca
     1861 gaacgaatta gagaattaga cactgatgct gctgaagaaa catatgaaat gtatgatatc
     1921 gatggaaaaa catatgcatt gccatatcga acggactttt gggtactata ttacaataag
     1981 aaaatgtttg atgatgcagg aatcgaatat ccaaaaaatc taacttggga cgaatatgaa
     2041 gatttagcaa aaaaacttag taaaaatgac ggacaagttt acggagcata tcaacatatc
     2101 tggcgttcga caatccaagc tattgcagct gctcaaaacg aggcgaatct tgttgaacca
     2161 gattatcaat atatgactga ttattatgat cgagcattga ggatgcaaaa agaaggcgcg
     2221 cagatggatt ttggcacggc taaatcaacg aaagtaacct atcagtctca atttgaagaa
     2281 caaaaagcag caatgatgta catgggaact tggtatatgg ctggcatact cgcaaacaaa
     2341 gaagcaagta agacagaagt agaatgggga attacagcta ttcctcagaa gaaaaaagga
     2401 gaatcggtca cgacgtttgg ctcaccaaca gcatttgctg tcaataaaaa ctcaaagaaa
     2461 caaaaagcgg cgcaagaatt tattgaattt gcagccagtg aagaaggagc aaaagtatta
     2521 gcaggagtag gagtagttcc ttcatatcgt acggatgaga ttgatcagct ttattttaat
     2581 ctcgctggaa tgccaacgga tgaaatttct aaaaaagctt tttctcctga tgaaatcaaa
     2641 cttgaatttc cgattgatac gtatgggcct gcaatcgata agattttaca agaagaatat
     2701 gacttagttt tggttggaga tgaaacgccg gaaaaaggaa cggtaaatat ggagaaacgt
     2761 gtagctgcgg aaaaagaata atcatgaaag gagagaagcc aagagatcaa acactcttgg
     2821 tttcgtcatc gaaatggaga atagtataaa taagaaagct acaaatcatt tgcgtagaaa
     2881 acgaacgtgg atcgcttatt cgtttatcgc cccgaatttc attggttttt tcgtttttac
     2941 attaatacca atcctgtttt cgcttgtact agcatttatg aaatgggatt ctttttcaac
     3001 gccagaattt gctggactag ataactttcg taaaatggcc aacgatgaga cattttggat
     3061 ttctttgaaa aatacttttt tatataccgc tgctgtagtg cctggcacac ttgtttgttc
     3121 gttagggcta gctttactac taaatcagaa aattagaggg atgaagcttt ttcgtacgat
     3181 atttttcttt ccgtatgtta cttccttagt agcaattgct gttgtttgga atatgttgtt
     3241 tcatccaaca gtgggaccta ttaatatctt tttaagacga tttattgaac atcctccagg
     3301 ttggacttca agttcaaatt gggcgctttg ggcaattgta attgtcagta tttggcgagg
     3361 gatgggttat tacatgattc tataccttgc aggactacaa agtatccaaa aagaactata
     3421 tgaagcgtct tcgctagatg gagcaaaccg ttggaaacaa tttttgaatg tgacattacc
     3481 ttcgttgaga ccaacaacct tctttgtaac gatcatgctt gtcatcaatt gtttcaaggt
     3541 gtttgacctt gtgcaagtga tgacggatgg cggccctggt cgagcgacaa atgtgctggt
     3601 ttaccaaatc tacaatgaag cattcaaaaa atttgatttt ggttatgctt cagctatttc
     3661 acttgttttg ttcgtaattg ttttgggcgt tacggttgtt caattttctt ggaataagcg
     3721 tcgtacttag gaggtgtgat atggaagaga gtgttcgaaa tgaaagaaaa aaagcaaaaa
     3781 ttcgttcaaa aaacatgccc tttattcaat ggttgggtat gattctgctg attgtattag
     3841 ctttattgac gatcattcct tttttatgga tggtttctgc ttcattcaaa gcaaataatg
     3901 aagtgttcac gattccgatt caatggatcc ctaaaacttg gcatttagag aattatctgg
     3961 ttatttggtc tcgtattcct ttagtgacat tttttaagaa tacagctttt ttaagcgttg
     4021 tgatcacgtt gattcagttg tttacttcta gttttgctgc ttacgggttt tctaaactaa
     4081 attttaaagg gcgcgatatt ttatttttag catatatcgg cacgattgct gtcccttggc
     4141 agtcttatat gatcccgcag ttcattatga tgagaaaagt gggtttaaca gatacgttgt
     4201 ggtcgttggt gctattacaa gcgttcaatg cttttggtgt gttcttgttg aagcaatatt
     4261 acagtagtat cccagattct ttatgtgaga gtgcgcggat cgatggctta tcagagtggg
     4321 aaatctatcg caagatcatt ttacctctta caaaaccggc gattgccagt ttgacgatca
     4381 tcacattcgt taatacatgg aacgattata tggggccatt catttatttg tcttctacgg
     4441 aaaataaaac aatccagtta gggttgaaaa tgtttattgg gctatttgat gcagagtacg
     4501 cattaatcat ggcttcctca gttgtttctg ttcttcctgt tgcttttata tttttagcga
     4561 tgcaaaaata ttttgtagaa gggatcgctt catcaggaat gaaggaatag gaggtgatta
     4621 tgaaagagca gcgttttgac aaaaatgttt atatggaatt cttttattgg ttgtatcaat
     4681 cattggcaag tagcctttgt ttttggtgtg tcaatatctt gtatatagca gctgcgttcc
     4741 ttttgtcgat agatggaagg aatgtctttt cctttatttt ttctttactt tttattggtc
     4801 caggttgcat cgtgatgata ggtatgacag aagaattagc aaaagaaaaa agtttttcac
     4861 cagtacgttt gttcataaag acgtttcgcc aattttggct aaagggattt ttttattggc
     4921 tatttgcttg gatagttagt gtgataatga tttttgattg tttctttttt atacgttttt
     4981 cttatggaaa atggttgatt ccgttgtttg ttttacttgc ttgtttatca gtttccttta
     5041 gcatcaactg ctggtatttt caggtgagaa atccagcgtc gaaaccaaat caagttctac
     5101 ggatagcgtt ctactatacg ttgaaaaaat ggtatgtgag tttactagat tttttactat
     5161 tgacaagttt gtttctcttc tttttcgtaa agccccaatg gtgtattcta ctaggtcctt
     5221 ctatcgtatt tggtttgatt tacttcaaca atcgtaaatt gatgagaact atggatctgt
     5281 agactttata aattgaaact ttcaattaag aatgacagtt ttttatgtaa aaactgtcat
     5341 tctttttcat tttttgaaac atatggctaa
//
LOCUS       DAGCXG010000123         5324 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.128, whole genome shotgun sequence.
ACCESSION   DAGCXG010000123 DAGCXG010000000
VERSION     DAGCXG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.128"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            5..1012
                     /locus_tag="MB022_002474"
     CDS             5..1012
                     /locus_tag="MB022_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4467442.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1024..2028
                     /gene="neuB"
                     /locus_tag="MB022_002475"
     CDS             1024..2028
                     /gene="neuB"
                     /locus_tag="MB022_002475"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBT4467443.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2025..3173
                     /gene="neuC"
                     /locus_tag="MB022_002476"
     CDS             2025..3173
                     /gene="neuC"
                     /locus_tag="MB022_002476"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBT4467444.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3146..3823
                     /locus_tag="MB022_002477"
     CDS             3146..3823
                     /locus_tag="MB022_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4467445.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3813..4955
                     /locus_tag="MB022_002478"
     CDS             3813..4955
                     /locus_tag="MB022_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBT4467446.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4968..>5324
                     /locus_tag="MB022_002479"
     CDS             4968..>5324
                     /locus_tag="MB022_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4467447.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTF"
BASE COUNT         1978 a          687 c         1038 g         1621 t
ORIGIN      
        1 ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga
       61 aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga
      121 agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat
      181 tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt
      241 tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa
      301 aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca
      361 ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa
      421 gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat
      481 attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt
      541 tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt
      601 tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt
      661 tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt
      721 aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg
      781 tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc
      841 atatgaaaat gataaaatgt atatcattcc accacactat catgctactg acgaactgta
      901 caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag
      961 tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt
     1021 atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta
     1081 gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt
     1141 caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag
     1201 gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa
     1261 tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact
     1321 ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt
     1381 ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca
     1441 gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc
     1501 atagatagtc cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat
     1561 attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc
     1621 gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt
     1681 gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat
     1741 cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat
     1801 ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta
     1861 gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa
     1921 cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa
     1981 agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta
     2041 tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac
     2101 aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat
     2161 ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt
     2221 ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata aatggtttca
     2281 caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa
     2341 tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag
     2401 gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg
     2461 ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt
     2521 ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta
     2581 gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc
     2641 aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga
     2701 tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg
     2761 gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa aacggatatt atttatatta
     2821 cgaatttaga gccggaggaa tacctgacta tattaaaaga gtgcaaattt atgatcggaa
     2881 attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata aatttaggaa
     2941 ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta aatgaaaatg
     3001 aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg
     3061 ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa
     3121 tattgaatga tggggaagat attaagtgga ttcaaaaaca aatagcttat taaaatatac
     3181 tagtgatcca tacattatta ttggggctgg cggacacgca aaagtcatta ttgaaacctt
     3241 aagattagaa aataagttaa tatatggatt ttttgatgat aagcaaaata aaatgcaaga
     3301 tctgaaatat cttgggaaaa taaacgaatt gaatagctgg gcggatagca aaaaaaaaat
     3361 ctataaattt attgtagcta ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt
     3421 gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag tggcaaaaga
     3481 aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc cagatgctgt
     3541 aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt gtagaattaa
     3601 taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg taggtgtagg
     3661 aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat cttggtgtat
     3721 gataggcgca gggagcacta ttgttaagga tattccctca cattccttag cttatggcaa
     3781 tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta ttatcaagtc
     3841 ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat aattggatag
     3901 cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat actggttcta
     3961 agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc ttaagactgc
     4021 ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata gctacagtaa
     4081 atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct aatacatgga
     4141 atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac aaccaattac
     4201 ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa cgaattttag
     4261 ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg ttaggttcta
     4321 cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca tttaacggta
     4381 ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa aaatatgttg
     4441 agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat caacatagcg
     4501 aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt ggacaaatgg
     4561 aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat aaggaagctt
     4621 tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt
     4681 ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt aacgtaataa
     4741 ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta catttgcaac
     4801 cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg tcagaaaagt
     4861 tatttaatag cggcttatgc ttaccttctg gaagtaatat gacagatagt gaacaagaat
     4921 ttgttattga tgagtttaaa aaaatattca agtaggtgtg atattttatg aacataggaa
     4981 ttattggttt aggttccatg gggaaaagaa gacttagact cattcaagaa cactttaatg
     5041 atattttact atgtggtgtt gatactaatg aggcgagacg aaaggaagtt gaaaaggaat
     5101 ttggaatacc aacttataat agtctaacag aggcaaaaaa tttgtttgaa ataaattcag
     5161 tttttgtttg tacatcccct ataacccatg aatttattat aaaagaggct ttaaatttag
     5221 attgtaatat ctttacggaa atcaatttat taaatgaata ttatgatgaa ataatcgaag
     5281 tagctaaaaa gaaagataga ttactttatc tatcttctac attt
//
LOCUS       DAGCXG010000124         5253 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.129, whole genome shotgun sequence.
ACCESSION   DAGCXG010000124 DAGCXG010000000
VERSION     DAGCXG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.129"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(46..366)
                     /locus_tag="MB022_002480"
     CDS             complement(46..366)
                     /locus_tag="MB022_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4467448.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="MB022_002481"
     CDS             complement(514..2748)
                     /locus_tag="MB022_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4467449.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="MB022_002482"
     CDS             3227..3493
                     /locus_tag="MB022_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBT4467450.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="MB022_002483"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="MB022_002483"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBT4467451.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
BASE COUNT         1579 a          984 c         1056 g         1634 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttc
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaa
//
LOCUS       DAGCXG010000125         5226 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGCXG010000125 DAGCXG010000000
VERSION     DAGCXG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.130"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="MB022_002484"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="MB022_002484"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4467452.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="MB022_002485"
     CDS             2714..2938
                     /locus_tag="MB022_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467453.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3454
                     /locus_tag="MB022_002486"
     CDS             2951..3454
                     /locus_tag="MB022_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4467454.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            3580..4617
                     /locus_tag="MB022_002487"
     CDS             3580..4617
                     /locus_tag="MB022_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467455.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            4632..>5226
                     /locus_tag="MB022_002488"
     CDS             4632..>5226
                     /locus_tag="MB022_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBT4467456.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPK"
BASE COUNT         1915 a          736 c         1088 g         1487 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag caaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacggga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcctactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tgcgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc tgtcgatttt gatgaagtga aagaacagat
     3061 tggattaaat gacgaatatg aagagtacgc gattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acgccaattg aagagattaa tcggttgtgt aacttagcgg aggaactaga
     3181 aggaacgcca attggtgaag ctgcttctga gattcaacac gctttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatattgt ctattatcca gattgcgatg atatgagtga
     3301 tgtggcgcat tacttgattg tagaagctgg tgattttggc gaagttccag aacaattgaa
     3361 gccgtatatt gattttgatg cttgcggtcg agatttggag attggaggaa attatttggt
     3421 tactagtcgt ggagtttttg agtatccaga atgaatcaac actatatata gtgtttgatt
     3481 agtatatgta agcaaaaaaa cacaatatat agagtgcaaa ctgaagggta atgtgatata
     3541 atatttgccg aagaaatatt tttgaaatga ggtaatatta tggcaaatat tcaaacaagt
     3601 tttattgatt tccataattc aattagattg gacgtagaag acaatacgct acttaaggac
     3661 tataaagacc aagtaattga tggattaaaa gattacttac ctgatgatgt gaagtttgaa
     3721 acttttttac agggaagcta ttctgtttat accggaataa aaagctgtga tgaaaaaatt
     3781 gattttgaca tcgatattgc agttgctttc gaaattgatc atactgttta tgaagatcca
     3841 agggagccta aactgtgggt aaaagaggcg ctagttgaaa tttttcctaa tgctcaagtt
     3901 aatttaaaag taccttgtgt aactgctaca tttactggaa aaaagacaaa aaaaaatgtg
     3961 cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt atttcttagc aaaagcaaaa
     4021 gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg atcccaaagt attaaaagag
     4081 aaaattaata gtcatgtagc agattcagat gatcgaaaac aatttcgaag atgtattcgc
     4141 tatttaaaaa gatggaaaga taacaatttt aaccaagaat ataaaccaac aggaattgga
     4201 cttacaatta atgtgatgga tacgttccta gttaataagt ccactgattt tttaactaga
     4261 aaagttcagt ataatgatat ggaatgtatg aagcagattg tttcttcgct gaaagattca
     4321 tttgtgtatg agtacagtga aacagatggt tggcattatc gtctacatgc atatcttcca
     4381 gtgaagccta acagtgacac ctattcaaaa atgacagtga atcagatgag tgattttaaa
     4441 aacaaacttt ctaaattata tgatgatttg attttcgcta tcgatactga agatgagtat
     4501 gaagctacaa aacgattaaa taatcaattt ggggaggatt ttttaattat ttcagaagaa
     4561 gaagttacag aaaaaaactt aagaaatgca tttgttacag actatccgag tgcttaggtt
     4621 ggtgttaaag attggattat actgatattt taaaagaaaa gctaacagat gttgtgcgtt
     4681 tattcgatgg taattactat ggggattttt catttgctac aattggaaag ttgcgtttaa
     4741 aaattgaaat accaaaacat tttccattgg tacttcctag gatttacatt cataattttg
     4801 atcatttagg actttaccta ccacatgtag aaaaagatgg gttggtatgt ttttcttcag
     4861 atataaatta tttagtagat gaagaaaatt cagaattggt tttattgcag tgtgttaata
     4921 aaactataaa aacaattgag gaaggtttga aagggttaaa taaaaatgat tttcgaaaag
     4981 agtttgttgc tttttgggac aaacaagaga atcaaatttc gttattctac ctttgtgaac
     5041 cagcgttgtt tatcaagtca gttgatacaa taatgtacgg taagtctttg gttgctttgg
     5101 ataaagagaa cgaattacaa ttaaatgcag cgagtagatt gtttaatgga gagaaagaag
     5161 atactcatga attagatgca tactatgttc caattagaaa caagaataat attatgcccc
     5221 cgaaga
//
LOCUS       DAGCXG010000126         5110 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGCXG010000126 DAGCXG010000000
VERSION     DAGCXG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.131"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..182)
                     /locus_tag="MB022_002489"
     CDS             complement(<1..182)
                     /locus_tag="MB022_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4467457.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="MB022_002490"
     CDS             517..951
                     /locus_tag="MB022_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4467458.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="MB022_002491"
     CDS             974..1294
                     /locus_tag="MB022_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467459.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="MB022_002492"
     CDS             1435..1626
                     /locus_tag="MB022_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467460.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="MB022_002493"
     CDS             complement(1697..1954)
                     /locus_tag="MB022_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBT4467461.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="MB022_002494"
     CDS             2112..2375
                     /locus_tag="MB022_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467462.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="MB022_002495"
     CDS             2399..2587
                     /locus_tag="MB022_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467463.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="MB022_002496"
     CDS             complement(2670..2855)
                     /locus_tag="MB022_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467464.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="MB022_002497"
     CDS             3070..3441
                     /locus_tag="MB022_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4467465.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="MB022_002498"
     tRNA            complement(3591..3663)
                     /locus_tag="MB022_002498"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="MB022_002499"
     CDS             complement(3709..4137)
                     /locus_tag="MB022_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467466.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="MB022_002500"
     CDS             complement(4197..4955)
                     /locus_tag="MB022_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4467467.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1783 a          789 c          993 g         1545 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt
//
LOCUS       DAGCXG010000127         4996 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGCXG010000127 DAGCXG010000000
VERSION     DAGCXG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.133"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..541)
                     /locus_tag="MB022_002501"
     CDS             complement(<1..541)
                     /locus_tag="MB022_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4467468.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSN"
     gene            794..1069
                     /gene="rpsP"
                     /locus_tag="MB022_002502"
     CDS             794..1069
                     /gene="rpsP"
                     /locus_tag="MB022_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBT4467469.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            1081..1332
                     /locus_tag="MB022_002503"
     CDS             1081..1332
                     /locus_tag="MB022_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBT4467470.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            1456..2247
                     /locus_tag="MB022_002504"
     CDS             1456..2247
                     /locus_tag="MB022_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467471.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAADSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            2417..2938
                     /gene="rimM"
                     /locus_tag="MB022_002505"
     CDS             2417..2938
                     /gene="rimM"
                     /locus_tag="MB022_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBT4467472.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2928..3689
                     /gene="trmD"
                     /locus_tag="MB022_002506"
     CDS             2928..3689
                     /gene="trmD"
                     /locus_tag="MB022_002506"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBT4467473.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3825..4172
                     /gene="rplS"
                     /locus_tag="MB022_002507"
     CDS             3825..4172
                     /gene="rplS"
                     /locus_tag="MB022_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBT4467474.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(4293..>4996)
                     /locus_tag="MB022_002508"
     CDS             complement(4293..>4996)
                     /locus_tag="MB022_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4467475.1"
                     /translation="LLFSYAMKKGYVEKNPIDEVVIDYKRESKTIKIKDKFLEDDEYN
                     RLVDFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVINGTMMYRER
                     SIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQLTAIN
                     TYLRNHKADMKIDKKLSSHIFRHTHISKLAELETPLYAIQDRVGHENSDITEKIYLHV
                     TKGVKEKLKEDIEKL"
BASE COUNT         1663 a          908 c          960 g         1465 t
ORIGIN      
        1 tgttagaaaa atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa
       61 cgatattacc attttcatca attagattcg caacgtttcc gatatatgct tccggttgct
      121 gcaaggttgg cacattcaaa aagacaagtg attgtcgtag atggtggttt gcgccaaagc
      181 cgccgatccc acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt
      241 gtccataagg tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt
      301 tgtattcagg agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag
      361 tccattcagc aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat
      421 cgatttttac aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta
      481 cttttttgtt gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca
      541 tacgattttt cctccttcaa actatctata acaatcttac aaaaaataag taactggagc
      601 aaataaaaac acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa
      661 aagaagtcta aaatcttcta tttcctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa
      721 aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag
      781 gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc
      841 gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac
      901 tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt
      961 tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa
     1021 agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat
     1081 atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat
     1141 caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct
     1201 cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca
     1261 atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat
     1321 aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt
     1381 attaattgaa gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga
     1441 gggagtgaac agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc
     1501 gcttgtagtt cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa
     1561 actgttacca gtccagaaaa aagtagtacc aaaacggcta ccaccactga acaaacaatg
     1621 gctacctcaa ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat
     1681 gttcccatgc ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat
     1741 acaaagcaag gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct
     1801 gacgaattga atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct
     1861 agtcaagaaa caatccaagt tttgcagccc tttgagatcg acacaaatgg tcagcaagtg
     1921 gacttaggtt ctcgaatcac agggtatcag caaggtgcag cagattcaag ttttcttgaa
     1981 tggcaagaag gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc
     2041 ttactattag ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa
     2101 caatttggta aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc
     2161 tggcaagaac cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag
     2221 atggcagttt ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac
     2281 tccaagtcac aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa
     2341 taccttaaaa gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa
     2401 gaaatgggga tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg
     2461 tatcaaaggc gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa
     2521 aggaacggtg ttaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag
     2581 tcatcgcaaa cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga
     2641 tgtggaaaaa tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc
     2701 tgaaaatgaa ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga
     2761 gttaggtaaa gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg
     2821 tccgaaaaaa gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt
     2881 agaagctgga gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg
     2941 tattgacttt atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg
     3001 ctgtagataa aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca
     3061 aacatcagac agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc
     3121 agcccatcta tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag
     3181 taattcttct tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac
     3241 aagaagaaca cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca
     3301 cattagtgac agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg
     3361 cgatggtcat gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt
     3421 cagctcaaac ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg
     3481 cagaatataa agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg
     3541 aagaatggca attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg
     3601 aaacacttga atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac
     3661 aagaaaaaac agcagatgaa gccaagtgac tttttctaaa atattcttta cagtaggttg
     3721 ataatatgtt agtattttat ggtgagtgca aaagcactta actattacga tattccgctg
     3781 tgagaaaagc tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg
     3841 aagaattaac aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg
     3901 tgcgtgttca cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg
     3961 ttgttatcaa acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta
     4021 acggagtggg cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag
     4081 tagttcgcta cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa
     4141 aagctgctcg catcaaagaa atccgtcgtt aatcatcgtt atcagactga gaaagaaccc
     4201 ttggtattca agggttcttt ttttgttatc tgttttttta tattaacgat ttcgatttgg
     4261 tacatttttg attaataaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
     4321 ttttctttta ctccttttgt cacatgcaaa taaatctttt ctgtgatatc actattttcg
     4381 tgaccaacgc gatcctgaat agcatacaga ggagtttcta gttccgctaa ttttgaaata
     4441 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
     4501 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
     4561 agaaattggc cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
     4621 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
     4681 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc gttattattg
     4741 gtaatgtgta catcgtcttt gcttaatgcg attgcttcac caggcctcat cccagtcaaa
     4801 tatagccact ggaaaaggag agaatatcgt ttattgtgtg aagttgtgaa atctactagt
     4861 ctattatatt catcatcttc taaaaattta tctttaattt ttatcgtttt tgactctctt
     4921 ttataatcaa taactacttc gtcgatagga tttttttcta cataaccttt tttcatagca
     4981 tatgaaaaaa gaagat
//
LOCUS       DAGCXG010000128         4932 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.134, whole genome shotgun sequence.
ACCESSION   DAGCXG010000128 DAGCXG010000000
VERSION     DAGCXG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.134"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            98..1045
                     /locus_tag="MB022_002509"
     CDS             98..1045
                     /locus_tag="MB022_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBT4467476.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1342..1890)
                     /locus_tag="MB022_002510"
     CDS             complement(1342..1890)
                     /locus_tag="MB022_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467477.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1891..2748)
                     /locus_tag="MB022_002511"
     CDS             complement(1891..2748)
                     /locus_tag="MB022_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4467478.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3034..3321)
                     /locus_tag="MB022_002512"
     CDS             complement(3034..3321)
                     /locus_tag="MB022_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4467479.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3311..3640)
                     /locus_tag="MB022_002513"
     CDS             complement(3311..3640)
                     /locus_tag="MB022_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4467480.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3717..3989)
                     /locus_tag="MB022_002514"
     CDS             complement(3717..3989)
                     /locus_tag="MB022_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBT4467481.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3989..4252)
                     /locus_tag="MB022_002515"
     CDS             complement(3989..4252)
                     /locus_tag="MB022_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4467482.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4358..4774)
                     /locus_tag="MB022_002516"
     CDS             complement(4358..4774)
                     /locus_tag="MB022_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467483.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4780..>4932)
                     /locus_tag="MB022_002517"
     CDS             complement(4780..>4932)
                     /locus_tag="MB022_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBT4467484.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1484 a          922 c          684 g         1842 t
ORIGIN      
        1 aatgatgctt atttttaaaa atttaaaaaa agactaaatt caattttttt ggtataatga
       61 gaaaaaatat ctatttgaaa aagttaggag ctatactatg catctggaat tctctgacta
      121 tgaatcactc cttgtaaaaa tttgtgaaga ctattatctc gaggatatca atattagtga
      181 aatttctaaa aaatatggga tcaccagata taaagcttta aaatatattg aagatgcacg
      241 tgaaaaacaa ttagttagta tttcaattaa ttctccgtat gctaggaatt atgagctaga
      301 agaaatcttc aaaaattact ttaaaacgaa tatctatata ttaaaaagta gtgaagactt
      361 atcacaacac gatttattat tttcaaaatt tgctgccgat tacattcaag aattaattaa
      421 gaaagccaat gtggttgcac tatcttgggg agataccgtt tacaaagtga ttgatcagtt
      481 taaaatgggt gctcgtgaag aattaatttt tactcaattc attggcgaaa tcggaaaata
      541 taattcctta gctggctcca cacgattagt tcaaaaagct gctgaacgtt atgagtcttc
      601 ttatttgact ctcagtattc ctttatatgt catcaatgaa actgcacgtg aactaatggc
      661 tctagaacct tctttagcta ataccttagc aaccgcttcg catgcagata tgttaatttc
      721 tggaattggc accttatcct ctattgattc tgtcgaaccc tggaacaagc atcgtaaaat
      781 cctatttcca agcatgacgg atactgttgg atttctttac ggaagaccct tcaatatcaa
      841 tggtgaattc atcaaagcat ctgaggataa gacttttggt ctttctttaa aagaaatttt
      901 cgatattcca aaacgaattg gtatttgtaa aagtaagttc aaagcggaga gttgtttagg
      961 cgccttgaat ggtaattttt ttacagattt atttttggat gaaaaaactg cttggaaaat
     1021 actttctttg ataaaaaaag agtgagtcat gtaaaatcct ccaaaaaatt tagatttttg
     1081 gtactacttt tttggagggc agtacaatga ttcactcttt ttattggata tcaaaatccc
     1141 tttttttgag aagcttgtct tgatattttt tgaacttttc caagccatac tccattactt
     1201 ttcaagattt aatttctttg tattcactga ataatttcca cgttttttcg ttaatgttac
     1261 ttcttattaa ttttattgta cttctcatgc aataggatca tatatccttc taaatctttt
     1321 ttatgctata aaaagggggt tctatctaac tttattattc attccccacc ctttgcgcag
     1381 tgagtcttcg gtatcttgtt ttatctgact ggtagttctt attatttctc cacggtcatc
     1441 taattctgga acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt
     1501 aaacaaatct tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt
     1561 tctaagcaat tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt
     1621 cgcatttaac aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt
     1681 tctgattggt gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga
     1741 agtttttatt tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc
     1801 ttgctgcttt ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc
     1861 aaaaatatca agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg
     1921 ttcttccaat tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt
     1981 taagagacct ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg
     2041 cgcatttttg agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt
     2101 atacatttcc tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac
     2161 atataaattt gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc
     2221 atttctcatc gattttactt gggtctgtaa gacgacgatt gcccagtccg caatgtacat
     2281 cgcggattta ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc
     2341 atcaaataat ggttctagct tttctttcat atatttgaat tgggataatg aatcattata
     2401 ttggtctttg atccaatcat catacatttc aaaggacata tctgctggaa gtatatagag
     2461 gttattgatt actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt
     2521 cacagtattt tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt
     2581 catatgcgtt aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa
     2641 ggttcttttt ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt
     2701 acctacgcca cctttaaaat taccaattac atatttcttc gctaccacgt tcctacctct
     2761 tttcttttag caatattcat ttgatactat tataacatat ttatttttag ttactaccaa
     2821 aaaaaagata tatatgtact accttataat taataagtaa aaggtagtac ataaataagg
     2881 aaaagttgaa atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc
     2941 gagaagcaga gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa
     3001 gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata
     3061 aaagttttca tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta
     3121 agctactcta tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc
     3181 cccagttttt tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa
     3241 aaatttttct ttcaatggct tctcttttag cttttttaaa tacttaatga catgttttga
     3301 aggaacgatt tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat
     3361 tagtaagtgt atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct
     3421 taaattctac aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat
     3481 cttcgctaaa atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct
     3541 ctaccaactt tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca
     3601 acatgattga attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac
     3661 agtatgtaat tacttagtaa atacattata ccacgagata atgtatttac aagtaattac
     3721 tgtgactcgt aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc
     3781 ttataaacaa cccggtgttg tctattcaat cttctagaat acctaccttc atatgtaggt
     3841 tttaacttct caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc
     3901 ttctcaaatt gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag
     3961 tttttaatca tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat
     4021 tagtaaatcc actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct
     4081 caagtaatag cgtttctttg attgctttca aatcatccaa accaataagc accgcattat
     4141 tttcactgcg ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca
     4201 acttataaaa atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc
     4261 cccctctaat ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt
     4321 tgtttaaatt ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatttttat
     4381 taacaagttt tatcacaggc ttcatatatt tgattataac ttcatcttct acgtagatat
     4441 ctgttatcat tacgacatct gtactatttc gattgagtac tttcacttga tctccagcat
     4501 tgattttccc tctaaatctt ttaggaatga accaactata catatgatta gcatcaccaa
     4561 aatgtgcttt tatcgcattc tcaggatata acttattcaa tagttcattc tttttaattt
     4621 tcaaaacttc aaatctatac ttatcatcat ctgctaatct aaataaatct tctaaacgca
     4681 tgttttctaa ctgtaagttt tgaaaatctt ccggtataaa ttttcggttc tgttgcgaag
     4741 tttgaaaatc aaacgcgccc tctctgaact ccaaatatct taggctgtta ttcccattaa
     4801 taccttgatt tcagtagaca ccgaaaagct gaagagcgtt ccatttcttc ggttcttttt
     4861 atatattcct cgaatggtct ccatgccctt aatcgtggaa gaggctgtac ggagactttg
     4921 ataaaattta tt
//
LOCUS       DAGCXG010000129         4912 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGCXG010000129 DAGCXG010000000
VERSION     DAGCXG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.135"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..244)
                     /locus_tag="MB022_002518"
     CDS             complement(<1..244)
                     /locus_tag="MB022_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467485.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
     gene            430..1365
                     /locus_tag="MB022_002519"
     CDS             430..1365
                     /locus_tag="MB022_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4467486.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1450..2460)
                     /gene="gap"
                     /locus_tag="MB022_002520"
     CDS             complement(1450..2460)
                     /gene="gap"
                     /locus_tag="MB022_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4467487.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2754..4061
                     /gene="obgE"
                     /locus_tag="MB022_002521"
     CDS             2754..4061
                     /gene="obgE"
                     /locus_tag="MB022_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBT4467488.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            4282..4869
                     /locus_tag="MB022_002522"
     CDS             4282..4869
                     /locus_tag="MB022_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBT4467489.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
BASE COUNT         1550 a          850 c         1097 g         1415 t
ORIGIN      
        1 ctttaatgta atctacttca tgagcaggta attgaatgac ttttctacca cattttttct
       61 tattttcttt atactggtgc caataatcaa gagcagtctt gccttgcttg aacttattga
      121 ctacattata aatagtttgt atagcacgtc ccattcggtt agcgatttca accggcttat
      181 tatgttgaag ataatatgat tctatcattg tcagttcgtc tatggtaaga tgtttgtagg
      241 tcatttatgg ttacactcct ttgttttctt tcgtcggaaa tacaatttga gtgtaccata
      301 aatgcctttt tatttttcta acttaatttt acaattcgcg ggttataaaa atgaacattg
      361 catagtagag attctttgat tatactaatt caaggttata tgaaaaaaga gaaatgagat
      421 ggatgattta tgaaactttg gatgaaaatc cttttaggcg tcgctttagc tctgattgtt
      481 gcgttagtgt ttgcagggaa ttatttttat aattatgctg ttgtgccttc tgagaaagac
      541 tttttagacg aagatacacc tggtacaaca cagacatcca aagaaacgga tgcacataaa
      601 tggtttagtt caaaagaaaa ccgaagcgat tggaccatca cttcaaaaga cggtctgaaa
      661 ttatcagcga tttatcttcc tgctgaaacg aaaagtgaaa aaacagctct tgtggctcat
      721 ggctatatgg gaaacgctga aacgatgacc aattacgcaa agatgtttca tgatatgggt
      781 tacaatgtcc ttgttcctga tgcaagagga catggaaaaa gcgaagggga ttacattgga
      841 tttggatggc cagagcgtaa agactatgta cagtggataa acaaagtgct agaaaacaat
      901 ggaaaatcgc aagaaatcgt tctatacggt gtcagcatgg gagcagcaac agttatgatg
      961 actagcggag aaaaactacc agataatgtg aaagcaatca ttgaagattg cggttattct
     1021 tccgttcatg atgagcttgc ttatcagcta gacgatatgt ttagtcttcc agcatttccg
     1081 cttatgcaag taacgagtct agtgacaaaa gtccgtgctg gctacttttt cggtgaagca
     1141 aatgcagtgg aacaattgaa gaaaaaccag cgcccaatgc tctttataca tggagacgaa
     1201 gatacatttg taccatttga aatgttagat aaggtctatc gtgcaacgaa gggaccaaaa
     1261 gaaaagtatg ttgtaaaagg agccgagcat gcggaagcat acaagacaga tcctgaaaaa
     1321 tatcagcaag tagtccaaca atttttaagc caatatgttc attgaacgag tttaaaagta
     1381 ggggatgaaa agaaagaagc ctttgactgg atccagtcaa aggcttcttt tatcgattga
     1441 tctatcaaat tacaagttag cgaatttttc tagtaagcga atcatttggc aagtaaatcc
     1501 atactcattg tcataccatg caacagtttt aacaagttgg aaatcaccag cagttgttac
     1561 ttctgtttgc gttgggtcaa agatcgaacc ttgagttgtt ccaatgacat cgctagaaac
     1621 gatttgacgg tcatcatacc cgaatgaagg attgtcaact gtgtgttttt tgattgcttc
     1681 gtttacttga tcagcagtta cttttgtttt caatactgaa accaattcag ttaatgaacc
     1741 atcaacgact ggaacacgtt gtgcgtgtcc ttgtaatttt ccttgtagct cagggataac
     1801 taagccgatt gctttcgcag caccagtaga atgtgggata gtattgtcag cagctgaacg
     1861 agcagcacgc aaattaccgc cttttacagg tccatctaag agcatttgtg tggaagtgta
     1921 ggcatgaact gttgtcattg taccaacttc gatcccaaat tcattattta agaaataagc
     1981 cataggtgct aaacagtttg ttgtacatga acctgctgag atgattttgt cgttttcatc
     2041 taatgtatca tcattgacgt tatagacgat ggttttcata gcaccagctg gggcagaaat
     2101 aactactcgt ttgacaccag catctaaatg cgcttttgct ttttcttcag atgtataaaa
     2161 accagtacat tctagtacga tatctacacc gttttctttt gcccaaggga ttttgcttgc
     2221 gtcgggttct gcataaacac gagttgcttc gccatccact acgattgagt cttctgttgc
     2281 agtaactgtg ccaggataag taccatgagt agagtcaaat tgcaataatt gtgctaacat
     2341 agttggactt gttaagtcgt tgattgctac tacttcaata tcatctgaga cttctttgat
     2401 gcggcggaaa gccagacggc caatacgccc aaatccgtta atacctactt ttactgtcat
     2461 atcaaaagtt cctcctttat taggggtgct gtgaaacggc atcctcaatt aatacaatta
     2521 atacactgtt attctgctac gcaaagaata ttttgtcaaa aaattatact tggtagcaga
     2581 aaaaaggttg gaaaccttac caaatgataa aaaaagaaga aggtaaacaa tctttttgct
     2641 gaaatgatag acaaaatcag atttcaccga taaaataagt aagacttttg tttattgtgg
     2701 aaattcaggc agtcaaataa ataaatgaat aataggagga cttaattatg tccatgtttt
     2761 tagatcaagt aacgatcgat gtgaaagctg gcaagggcgg agacggaatg gttgcatttc
     2821 gtcgcgaaaa atacgttcct gacggcggac cggctggcgg agacggtggt cgtggcggcg
     2881 atgtcatctt gatcgtcgat gaagggttac gtacattgat ggattttcgg ttcaatcgcc
     2941 atttcaaagc gcagccaggt gaaaatggga tgagtaaagg gatgcacggt cgtggttcag
     3001 aagataccta tgttaaagta ccacaaggaa ccactgtccg agatgcggag actggtgctc
     3061 ttttaggaga tttaattgaa aatggtcaaa cactagttgt ggcaaaaggt ggacgcggtg
     3121 gacgagggaa tatccgtttt gcctctcctc ggaatcctgc accggaaatt gctgaaaacg
     3181 gtgaaccagg acaagagcga aaaatcgaac tagaattgaa agtattggca gacgttggac
     3241 ttgtaggttt tccatctgtc gggaaatcga ctttactatc cgttatttca tctgctcgtc
     3301 caaaaatcgg cgcttatcat ttcacgacgc tcgtacctaa tctaggaatg gttactacaa
     3361 gtgatggcag aagttttgcc gcagcagact tgccaggctt gattgaagga gcttcgcaag
     3421 gtgttgggct gggtacacaa ttccttcgtc atatcgaaag aacacgagta attttacatg
     3481 tgatcgatat gagtgggatg gaaggacgag atccttatga agactatctt gccatcaaca
     3541 aagaattatc tacttataac ttacgcttgc tagagcgtcc gcaaatcatc gtggcaaaca
     3601 aaatggatat gccggatgca ccggaaaatc tagtgaaatt caaagaacag ctgaacaagg
     3661 aaaaagaaga tgaatttgct gatgacatcc cagtatttcc aatttctgga gtgactcgac
     3721 aaggattgga cgcgttattg aatgctacag cagatttgtt agaagtaacg ccagaattcc
     3781 cattgtacga agaagaactg gaagaagaaa ctgtacacta tggattcaat ccagaaggtc
     3841 ctgagttcca aatcgatcgt gattcagatg caacgtggat cttatctggt gagaagatcg
     3901 aaaaattatt ccaaatgacg aatttcgatc atgatgaaac agtgatgcgt tttgctcgtc
     3961 aattacgtgg tatgggtgtg gatgaagctc ttcgggcacg cggtgcgaaa gatggcgacc
     4021 ttgtacgtat cggtgagttt gaatttgaat ttgtcgaata ggcaagcaaa taaaaacaaa
     4081 taccaaaaaa tatagattca gatttaaggg aggttgtgac agaagtgatc agcttcaaga
     4141 acgccgttta ttcggaaaaa ggagctgtga tacgactttt gtcacagccc tattttattg
     4201 gaaaaaaaga gtgcttttct tttcttattt ttcaattaaa ggtaaaataa ataacaagaa
     4261 ggtatgaaag gggcgttttt aatgagcaaa cgagtaatca ttttaaattt tcaagtagaa
     4321 agccaggcat atcaggcatt ttcagaaatc aaaaagttac atgctgttcg ttcgattaaa
     4381 ggagaacaga tggcagttgt cagccatgat cctaatggga cacatcaatt caaaatagaa
     4441 gattttatcg actttaccgg taataacaaa tcttctacgg gtggattaat cggaatgttg
     4501 gttggtgtat taggtggtcc gctagggatt ttattaggat ggtttgccgg aggaatgatc
     4561 ggtgcgacac aagatgccaa agaaataaaa gatgcccaga ccgtgttcga ctttgtagga
     4621 aataaaatcg gtgaaggagc tacaggactg atcttgattg ctgatgaaga ggacaatcgc
     4681 ccactgaatc aattggtaat gatggaatta ggcggagaaa ttactcgctt ggattatgaa
     4741 gatgtcgaaa aagatgtgca tgatgcgaaa gaagctgaaa atcatgcgaa agaagctgca
     4801 caaaagactt ggaaggacaa acatccgcaa acttccaacg acaatgaaag ttctggatct
     4861 tcaaattaat aaaaaacagc agaggatctc aaacgggttc gctgctgttt tt
//
LOCUS       DAGCXG010000130         4826 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGCXG010000130 DAGCXG010000000
VERSION     DAGCXG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.136"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            108..359
                     /locus_tag="MB022_002523"
                     /pseudo
     CDS             108..359
                     /locus_tag="MB022_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="MB022_002524"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="MB022_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBT4467490.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1739
                     /locus_tag="MB022_002525"
                     /pseudo
     CDS             1656..1739
                     /locus_tag="MB022_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1788..1871
                     /locus_tag="MB022_002526"
     CDS             1788..1871
                     /locus_tag="MB022_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBT4467491.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="MB022_002527"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="MB022_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBT4467492.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="MB022_002528"
     CDS             complement(2996..3859)
                     /locus_tag="MB022_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBT4467493.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="MB022_002529"
     CDS             complement(3861..4133)
                     /locus_tag="MB022_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBT4467494.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4359)
                     /locus_tag="MB022_002530"
     CDS             complement(4150..4359)
                     /locus_tag="MB022_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBT4467495.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..4600)
                     /locus_tag="MB022_002531"
                     /pseudo
     CDS             complement(4457..4600)
                     /locus_tag="MB022_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
     gene            complement(4654..>4826)
                     /locus_tag="MB022_002532"
     CDS             complement(4654..>4826)
                     /locus_tag="MB022_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBT4467496.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
BASE COUNT         1476 a          819 c          892 g         1639 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctg gttctgttgc aaagttttaa
     4621 ataaagaata aaatccctta cggtatctat gatttaagct gggattccca ataatacctt
     4681 gatttcagta cagaccgaaa acccgaagag agtgccttct tttcgggttt tcttatataa
     4741 tcctcgaatg gcttccatgc ctttaatcgt ggtagaggca gtgcgtaaac ttcgatagaa
     4801 tttattgcgt ctctttactg gacgat
//
LOCUS       DAGCXG010000131         4784 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGCXG010000131 DAGCXG010000000
VERSION     DAGCXG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.137"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            134..1180
                     /locus_tag="MB022_002533"
     CDS             134..1180
                     /locus_tag="MB022_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4467497.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1197..2420
                     /locus_tag="MB022_002534"
     CDS             1197..2420
                     /locus_tag="MB022_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4467498.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2509..3396
                     /locus_tag="MB022_002535"
     CDS             2509..3396
                     /locus_tag="MB022_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBT4467499.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3482..3712
                     /locus_tag="MB022_002536"
     CDS             3482..3712
                     /locus_tag="MB022_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBT4467500.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3769..4734)
                     /locus_tag="MB022_002537"
     CDS             complement(3769..4734)
                     /locus_tag="MB022_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4467501.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1615 a          795 c          971 g         1403 t
ORIGIN      
        1 aaagagctac gccagttttt ttgttctttt ttaagaaaac gtttttgtca gtaagaatag
       61 acaaaaatag ggcttaagtt tgagtgacag tttgtaggct atgttatact atataagtga
      121 agggggacgt aaggtgaagg tattattata ttttgaaagt gaaaagatgc ttgcaaaatc
      181 tggaatcggg cgtgcgcttg atcatcaaaa acgtgctctg acggaagtcg gtatctctta
      241 tacactagat gaaaaagaag attatgacat cctgcacatc aatacatacg gaatcaatag
      301 tcataatatg gtcaataaag ccagaagaga aggaaaaaaa gttgtctatc atgcgcattc
      361 tacagaggaa gatttccgaa attcctttat cggatcgaac cagctttctc ctatcgtaaa
      421 aaaatatctg gtaggattgt accaaaaagc ggactatctg atcacgccaa caccttattc
      481 aaaacagttg ttggaaagct atggtatccg tgtgccgatt caagctattt ccaatgggat
      541 cgacttagag aaatatcacc ctgatccaat gaaagaacaa aagttcagag aatatttcaa
      601 actgtctccg gatcaaaaag taatcatttg tgtcggtttg tttttcgaac gtaaaggaat
      661 cattgatttc gtagaaattg ctaagaagat gcccgaatac acatttattt ggtttggtca
      721 tgttcccatg tactcgattc ctcggaacat ccggaaaatt gtaaaagaag atcatcctga
      781 aaatgtcctg tttcctggtt acattcgtgg agagatcata gaaggtgcgt attcaggtgc
      841 agatctattc tttttccctt cttatgaaga gacagaaggg atcgttgttt tagaagcttt
      901 agcaagcaaa caaaacgtac ttgttcgaga tattccagtg tataatggct ggttagaaga
      961 tagcaagaac tgctatatgg ggaagacaaa cgaagaattt atccgactga tccaacagat
     1021 taccaatcaa gaactaccga gtacgacaca agccggttat caaacagcaa aagagcgaag
     1081 tatcaataaa atcggtgaag aacttaaaaa tgtatacgaa agtgtactga atgaaaaagt
     1141 ctctagcaaa ttaaaaaaag taaatcaggt caaggactag tttggaggcg aaaaacatga
     1201 aaatcggctt ttttaccgat acctactttc cgcaagtcag cggagttgct acatcgatca
     1261 aaacattaaa acaagaatta gaaagacatg ggcatgaagt ctatattttt acaacgaccg
     1321 atcctaatgc ggataagttg gaaaaagatg tgattcgaat gccaagtgta ccatttattt
     1381 ctttcaagga tcgaagagtt gttgttagag gcatgtggta tgcttatttg gttgccaaag
     1441 aactagaact agatcttatt catacccata cagaattcgg agcagggata cttgggaaga
     1501 tggtagctaa gaagttgaaa atacctctga tccatactta ccatacgatg tatgaagact
     1561 accttcatta tatcggtaaa ggaaaagtga tcaggcaatc ccatgtaaaa tacttgtcta
     1621 gattatttgc aaatcatacg acaggtgtag tttgtccaag tgaacgtgtt attgatacat
     1681 tacgcagcta tggtgtaata gcaccgcttc gcgtgattcc tactggtatc gacattgaaa
     1741 aattcaaacg ttctgatatc actcagcagg atatcagcaa attaagggag tcactaggtc
     1801 ttcaggaaaa ccatttgatg attctttctt taagcaggat ttcttatgag aaaaatatcc
     1861 aagccttgat taatgggttt cctcaggtca tctccgctta tcctaacgcc agattagtta
     1921 tcgtaggaaa aggaccttat gtagaagagt tagaggaatt gatctcagaa ctgcagttag
     1981 aagaatttgt gcagttcaca ggagaagtgg acaacaatga tgtagcactt tattataaag
     2041 cggcagatta ttttgtcagt gcttctactt cagaaacaca agggcttact tatacagaag
     2101 cgatggcagc cggaacacct tgtgtcgtgg aaggaaatgc ttatttgaac cgattgttcg
     2161 atcatccttc attagggaaa acttttgaaa ccgacgaaga ctttgcacca gcacttattg
     2221 attatatttc cagtgaagtt cctaaagatc caaaagtttt agaagctaaa ctttatgata
     2281 tttcatctac acatttcggc gaagctatga tcgaatttta tcaagacatg gttgtttatc
     2341 atgaagaaga gatgcgaaga aaagaagaag aagactcgat tgaacgaatc aaaataaaat
     2401 taaattcatt taaaagataa aattcaaaca ttttttatga aaaaatcctt gtttctagcg
     2461 tatactagca tagaagcaag gatttttgat ttttaggagg tcgaaaaaat gaaacttggt
     2521 tttatcggaa caggcgtcat gggaagtgct gtcgcacgcc atctactaga agcaggacac
     2581 gaagtagctg tctataatcg gacaaaagca aaagctgatc ctttagtaac agaaggagct
     2641 atctgggcag atacaccaaa agcggtagca gaacaaagta atattctctt tacaatggta
     2701 ggttacccca aagatgtaga agaaatctac tatggacagt cggggatttt ttctgcagat
     2761 atctcaggac atattttagt tgatttgact acaagtacac caagcctggc agaaaaaatt
     2821 gcaaaaacag caaaagaaaa aggcgcagat gcgttagatg caccggtatc cggtggtgac
     2881 ttaggagcaa agaatggtac actgacgatc atggtcggtg gagaagaagc cgtttatgat
     2941 caagtactgc ctttattcaa agaatttggt acgacattta cattgcatgg ttctgctggg
     3001 aaaggtcagc atacaaaaat ggctaatcag atcatgattg caggtacgat gacaggaatg
     3061 acggaaatgc ttgtctatgc acaaaaaaac ggacttgatt taaagaaagt gattgagacg
     3121 ctttcaggtg gaagtgcagc taactggtct ttaagcaatt attctcctcg tattttaaaa
     3181 gaagattaca cacctggttt ttttgtcaaa cattttatca aagatttaaa gattgcatta
     3241 gaagaagcag aaaaaatgga tcttgtattg ccagcaacta cccaagcatt gaaactatat
     3301 gaagagttgg cagataaagg ttttgagaat gacggaaccc aagctttgat caaactttgg
     3361 tggcctgaag gaaaaatacc agaaaaaaaa tcataaaaaa cgagtttttt tagagaaaat
     3421 caatgcaagt aggctatgct tttgatacaa tataacagaa ataagaaaag gaggacctcc
     3481 gatgacacat tcacaattag tcgcaatcat aaaacgactg gaggctatgg tcgagtcagc
     3541 agataatgaa ctgcaagttc gccgttttga aaaagaaggc gtagagaagt gtacagtaac
     3601 ttatgataaa gcgaccgata catttgaatt aactgaaaca gatactcatc agcaatacga
     3661 atttgataat atcgatatcg tagcgatgga aatctatgac ttaatccaat aaattttagg
     3721 ataattgaca tttgaagttg aagatgccta aaagaagccg agaagcaact aatttagttg
     3781 cttttcagct tcttttttgt ttaacagttc aatttcaaac tgttcttttt ctaatttttt
     3841 gcggtaactg atcaagattt gtacaggatc gatttcttct tttgacaatt gacgtttcaa
     3901 agataaatga gcttctttct tttcataata gaagtcagga gaaacgtcac cagaggtaat
     3961 cattgtttct gcaagttgac cataacaaac gaggtagtgc tttttctgac tctcatctgc
     4021 tgctaatggc atgctgaagg tgagcgatgg tgttcttaaa aactgctttt gttcctggat
     4081 ccgtctaata aaataagtaa ctaattcgat atctttttga agcataccgg ctttttcgaa
     4141 attcaactga tagatgaaat gttgttgtct ttttttcaag taagtcagta gctttttatt
     4201 tcggccttga aacagccctt ggagttcctt ttttttctgt tcaaaagtca tttcctgtag
     4261 gtcaggaagc tggactcgca aagctaacaa actaatttca gacaaccaag gcatttgata
     4321 agtttcttcc attatacgca aaatagacgg cattttttta tagagacgaa aaggcccgta
     4381 tgttcgagcc gtaggagtat ctgttaatac aaatccctta ttggtaatat gtacataatt
     4441 ataattactg aaataattca tttgtcggtt ataaaaagga cggtaatgtt ggatcaattg
     4501 acactcgagc aataaagcat ctaattccgt gtctactacg acgaaatcaa aatctgttat
     4561 atgatgaatc atacgtaata ctttttttga atgctgttta ttctgatgaa aatagctgct
     4621 tactctattt ttgagccgtt ttgctttacc aacatagatg accttaccat ctcggtcttt
     4681 cataagatag acacccggca gcatggggag agttttcatt ttttctttta acataggatc
     4741 tccttttacg ctttgaataa aaagactata acaaattttg aaat
//
LOCUS       DAGCXG010000132         4590 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGCXG010000132 DAGCXG010000000
VERSION     DAGCXG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.138"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            148..1137
                     /locus_tag="MB022_002538"
     CDS             148..1137
                     /locus_tag="MB022_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBT4467502.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELIESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="MB022_002539"
     CDS             1134..1553
                     /locus_tag="MB022_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467503.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1532..1870
                     /locus_tag="MB022_002540"
     CDS             1532..1870
                     /locus_tag="MB022_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467504.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1874..2236
                     /locus_tag="MB022_002541"
     CDS             1874..2236
                     /locus_tag="MB022_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467505.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="MB022_002542"
     CDS             2458..3864
                     /locus_tag="MB022_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBT4467506.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            4313..4432
                     /locus_tag="MB022_002543"
                     /pseudo
     CDS             4313..4432
                     /locus_tag="MB022_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         1598 a          651 c         1042 g         1299 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgatcgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagcttaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggccagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttca
     4021 aagggtgcgg ttttatgttt ttggatattt gcatagctaa agagaagtgc tattattgag
     4081 ttatcaaata aataaactga aaaaagagca aaaaaataaa gtcgagcaac gctggacacg
     4141 ttctcgacct ttacggaaac taatcacact agctccgtaa ctcaaattca ttatacgaat
     4201 aatttcaggt ttcttcaaga ctagagattg ctggttatga attttttaga gttacgtgaa
     4261 ctctggaaaa ttctttccac caatctcttt tttctttgag gaggaactaa atttgagtaa
     4321 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
     4381 tgtaaaaact ttttacaccc ccttaaaaat gcagtggtcg attgatcgaa ttttatgtct
     4441 ttgggttttg gggttttaaa acgaaaaaaa gggggcgatt ttcgccctct tttttgatgt
     4501 tttttcttat cttgatacta taggcaacta tttctgcgaa gaaatcgggg ctatagtatg
     4561 ggtctatccc ttgtggctct aaggaagaac
//
LOCUS       DAGCXG010000133         4587 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGCXG010000133 DAGCXG010000000
VERSION     DAGCXG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.139"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(52..522)
                     /locus_tag="MB022_002544"
     CDS             complement(52..522)
                     /locus_tag="MB022_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBT4467507.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="MB022_002545"
     CDS             complement(681..1139)
                     /locus_tag="MB022_002545"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBT4467508.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="MB022_002546"
     CDS             complement(1310..2455)
                     /locus_tag="MB022_002546"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBT4467509.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="MB022_002547"
     CDS             complement(2468..3385)
                     /locus_tag="MB022_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBT4467510.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="MB022_002548"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="MB022_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBT4467511.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1413 a          855 c          867 g         1452 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtagtcaa gtccacaatc ctgtgtaaaa tactatacaa
     4501 tgctctcact attttttact tggcaaattt aatatatttt cttgatgaag aaagccactc
     4561 atcgtgttga tcaataagga ctgctcc
//
LOCUS       DAGCXG010000134         4479 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGCXG010000134 DAGCXG010000000
VERSION     DAGCXG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.140"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..922)
                     /locus_tag="MB022_002549"
     CDS             complement(<1..922)
                     /locus_tag="MB022_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4467512.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKD"
     gene            complement(924..2363)
                     /locus_tag="MB022_002550"
     CDS             complement(924..2363)
                     /locus_tag="MB022_002550"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBT4467513.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLIHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2548..>4479
                     /locus_tag="MB022_002551"
     CDS             2548..>4479
                     /locus_tag="MB022_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBT4467514.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     WDYSEVNIVAENTVTEGTDL"
BASE COUNT         1406 a          772 c          750 g         1551 t
ORIGIN      
        1 aatccttatt tgtcttttcg cctttaattt ttttcagaag aatatcaaca ataagacttg
       61 caatttcatc aattggttgt cttattgttg tcaattgtgg gaaaaatttc tcaataaaag
      121 aggtaccatc gtatccaata atttttaaat cttctgggat attaagttta agttgatgag
      181 caattttcat agtgagaatg gctgttaaat catcagaaac aaaaatacca tctggtttgt
      241 gatttataat agttgacttt atttccattt cacgacggat agtagacaga ttattaggaa
      301 ttttgaaaat ttttccattt ggattttgaa aagaaaaacc tagtgcacgt aattctgtgg
      361 gagaatccgt gttatcattt cctgtaatca taatggtatt ttgacaacca tttttttgta
      421 aagtcttagc tgcaagtttt cccccttcaa agttatctga agaaacaatg gaaacattag
      481 gagctaaatt tctatcaaaa gcaacaatag gtgctctcac acgctcataa tcagcaattc
      541 caagattatg gcttgatgag attatgccat caacttggtt ggcttcaagc atttcaatgt
      601 attctctctc tttagcagga tcattttgac tattgcaaat aatcgtttta tatccatgtt
      661 taaataactc tatttcgaga tactcaatga gttcagagta aaagatatta ctaatattag
      721 gaaaaataag accaataagc tgaggtgact ttccttgaag tccacgtgct aaattatttg
      781 gtttataacc aagagttttc atagcttcat gaactttttg ttttgttttt tctgataaat
      841 atcccttatt attaatgacg cgtgaaacgg ttgttgggct aactccagct aattcagcga
      901 catcagttaa ttttgctacc attttaatat tttaattcaa agtattttcc agtaggttcg
      961 cctgatttga tttgaatacc attttggtga ttttcaggga atacacgact agtaaacact
     1021 ttttctcctt tattaataaa aatctcaatg attgagttat ctacaaaaat gttagcagtt
     1081 gtttctttag tatcaattga gcattcacga atcgtaccaa agtcagtggc atattgaatg
     1141 cctgctttgc tgcgatctaa aataattttt ccttctttgg tatctacaat caagctaagt
     1201 ccatttcctt tactatcaga gaaaagaagt aactccgatt tctgatttgg aggaaaagtt
     1261 agttcaagct cataagtatt atttgtttct gcttttgctg taaattgttc agaactagca
     1321 cgtaatgatg taatagcttc tactggatat tggtaaagtt taccatcttt tattgaaagt
     1381 tcttttacaa gactcattgc tccctgataa tcatacttat cagttggata atcgatatca
     1441 ggtagtccaa tccaactgac aatcagagca cgaccatcag gagtattgaa tccttgagtc
     1501 gcatacgctt caaatccata atcaagattt tgaatttctg aagcgcccac aagttttact
     1561 ttttctatgt caagcgattg acagatctta aaagtattcg gatagatatt cccataattt
     1621 agctcagatt tatccaaccc ttgaggacaa taaatgagaa caggtttttt gtctacaaat
     1681 actagatttg gacactcaat catgtattct gaaccagtgc cgccaaaatc aaggttgcca
     1741 acttcctccc atgtttccac gttattgtca tccgctttgt aaagtttgat aaagcctgat
     1801 ttttctaaat tttgtgctcc aacaatcgca tacaattgtc cttcataatt aaacatttgt
     1861 ggatcacgaa aatgatcagt tacatcagca ggtttatgaa taagaacatt ctcacctttc
     1921 gtaatgttgt agtctttatc catccatgct ccgatttgaa gtgtatcacg ttcccaattt
     1981 tcatttcgaa cgtttcctgt atagagtaag aatagattcc cctctatttc atatgcagaa
     2041 ccggaataag cgccgtggct atctcgtttt gtgtctggaa gaagttctgc tcctgttaca
     2101 tgaaaatgaa ttaaatcatc tgattcattg tgtacccatt ctttcaaccc atgagcagct
     2161 ccaaatggcc aattttgata aaaaagcgta tattttccgt taaaaaaaga aaaaccatta
     2221 ggatcgttta acaatcctgt tttaggttcg atatgatagc tagcacgcca aggtgatcgt
     2281 tttacattat ccttaatttt atccatctca tctttagtcc aatcagaata agcacgatat
     2341 cgtatttctc gaggtaagtt catattttac tccttcaatt tatcttttta caactagtat
     2401 ggtaaacgtt ttccgttgaa aaatcaatca tttcatttaa aaaaataaac tatatgacaa
     2461 acgtttgaca tatttttaag atatgctaaa ataaatttgc atttcagtga accgttttca
     2521 cgtaaaaata aaaaaggagt gattcgaatg aattacagta aagtagcttc cgacgtaata
     2581 caagcggtcg gaaaagataa ccttattgca gctgcacact gcgctacacg tcttcgtctc
     2641 gtactaaaag atgacacgaa agtgaatcaa aaagctttag acgaaaatcc agatgtgaaa
     2701 ggcacattta aaattgatgg acaatatcaa gtgattatcg gtgcaggtga tgttaatttc
     2761 gtttatgacg aacttatcaa aaaaactgga ctttcggaaa tttcaacaga tgatttaaaa
     2821 caaattgcta gtaaagataa aaaattcaac cccattatgg cattaattaa attattatca
     2881 gatatttttg ttccaattat acctgccctt gttgcaggtg gtttattaat ggcattacaa
     2941 aactttttaa cagctgctgg actttttgga ccaaaatcaa ttgaagaaat gtatcctgcg
     3001 attgaaggta tttcctcaat gatccaactg atgtccgctg caccattcat gttcttacca
     3061 attttagtag gtatttcagc tgctaaacgt tttggagcaa accaatttct tggtgctgca
     3121 attggtatga tcatgacaac accagatctt ggtggctcaa ctgaatattg gaatattttt
     3181 ggttatcatg tttcacaaac aaactatgct tatcaagtta ttccagtttt agcagctgtt
     3241 tggatattat ctatattaga aaaattcttt cataaaaaat tgccatcatc tattgacttt
     3301 acattcacgc cattattatc agtaatgatt actggatttt tgactttcac agttattggc
     3361 cctgtaatgt tattgttgtc taatggaatt acagacatta ttgtttggct atataacact
     3421 actggattta ttggaatggg tatttttgga ggaacatatt cacttatagt catgactggt
     3481 ttacatcaat catttccagc tattgaaact caattacttt cagcgtggac aaatggtatc
     3541 ggacatggag actttatctt tgtagttgca tctatggcaa acgttgctca aggtgctgca
     3601 acatttgcca tttggttctt aactaaaaat tctaagacaa aaagtcttgc ttcctctgcg
     3661 ggtgtctcag cacttcttgg aattacagag cctgccttgt tcggagttaa cttaaaatac
     3721 cgctttccat tcttttgtgc tttaattggt tcaggaattg ctacagcagt agcaggatta
     3781 cttaaagtag ttgctgtttc acttggttca gcaggtttct tgggatttct ttcaattaat
     3841 gcaacatcta ttccatttta cgtattgtgt gaattgatta gctttgtagt tgcttttgca
     3901 attacttact tctacggtaa aacaaaagct gctggtatct ttaaagcaga agaaattgct
     3961 gaacaaacac cagttaattc ttcaaaagta gcaacaacag atccaatatc tactgatgtt
     4021 gaatcaacac aaacaaaagt ttcatcagaa acaattgtta gtcccatttc tggtaaagtg
     4081 attagtcttt cttcagtaaa tgatccagtc ttttcatcag aagctatggg aaaagggatt
     4141 gccattaaac caaatggaaa cacggtccat tctccagtag atggtactat tcaactcacg
     4201 tttgaaactg gacatgctta tggtattaaa tcaaataatg gtgctgaagt tctaattcat
     4261 attggtattg atactgtatc aatgggagga aaaggattta cacaaaaagt tactactaat
     4321 caaaaagtta gaaaaggcga tattcttgga gcatttgacc gttcaatgat tgagaaagaa
     4381 ggtcttgatg atacaacaat gattattgtg accaatactt gggattattc agaagtaaat
     4441 atagtagctg aaaatacagt cactgagggt actgattta
//
LOCUS       DAGCXG010000135         4444 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.141, whole genome shotgun sequence.
ACCESSION   DAGCXG010000135 DAGCXG010000000
VERSION     DAGCXG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.141"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..423)
                     /gene="dfrF"
                     /locus_tag="MB022_002552"
     CDS             complement(<1..423)
                     /gene="dfrF"
                     /locus_tag="MB022_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBT4467515.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGET"
     gene            complement(842..1060)
                     /locus_tag="MB022_002553"
                     /pseudo
     CDS             complement(842..1060)
                     /locus_tag="MB022_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1091..2305)
                     /locus_tag="MB022_002554"
     CDS             complement(1091..2305)
                     /locus_tag="MB022_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBT4467516.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(2646..2888)
                     /locus_tag="MB022_002555"
     CDS             complement(2646..2888)
                     /locus_tag="MB022_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4467517.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(2920..3477)
                     /locus_tag="MB022_002556"
     CDS             complement(2920..3477)
                     /locus_tag="MB022_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4467518.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(3490..3678)
                     /locus_tag="MB022_002557"
     CDS             complement(3490..3678)
                     /locus_tag="MB022_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4467519.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(3691..4257)
                     /gene="amaP"
                     /locus_tag="MB022_002558"
     CDS             complement(3691..4257)
                     /gene="amaP"
                     /locus_tag="MB022_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4467520.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1315 a          952 c          693 g         1484 t
ORIGIN      
        1 tgtttcccct atcaaaactt caaaatcatt gatatcaaat tctggaaaga atgtatctcc
       61 atcttcaata tttaaatcta cttctgtgat atacatttta tctactattt gcaaagcttc
      121 cttaaatagt ccatatccac cagatatgta tacatctcgt cctttagcca ataataatgc
      181 atcttctaat gatttaactg aaactaaatt atctccttga tactctgttg tggtggaaac
      241 aacaatattc attctattag gcaacggatg accgatttct tcataagact ttcgccccat
      301 aataaccaca ttacccgttg ttaactctct aaattgcttt tgttctccct ttattttcca
      361 tggtatatta ccattcttgc ctataacatt attctttgac ctcgcaacaa tcaaacctat
      421 catctatttc atcctccata atatgatgtc gtacctgtga ttccatcaag tgcaccacat
      481 cttaacaaac tcaaaattat ctcgaactaa ccagctcgac aaattccttt tttgtcttca
      541 tctgcattat atcatattgt tataagcatt gccatttctt ttctgctttt gaaaagttac
      601 ttatcagcct atgttttgta aagggtgata ttcagcattt ctcacgcaac aagcccttga
      661 caaaccatag actaataagt tttaaggtaa tcaagcagaa atgaaatagt ctgctgtttc
      721 acggcaacaa aaaagagata ccaacacact tatgtagtat ctcttagtct agtagcaata
      781 ttcttttgtt tgcaagcagg taaacatggg ctgtttctta atatcttttt gtttgggaaa
      841 cacctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
      901 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
      961 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg
     1021 ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt
     1081 tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt
     1141 cttcttttgt agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca
     1201 gcacataggc atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc
     1261 agctgcttgg attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac
     1321 ctgtgaatcc gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct
     1381 ttttacgaaa atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg
     1441 cactttttgc ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc
     1501 ctgttagtac gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag
     1561 taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat
     1621 ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac
     1681 cgatagcagc tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt
     1741 gaccaagtgt gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc
     1801 caccagcgaa atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc
     1861 aaacagggaa ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat
     1921 tcaatctccc tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt
     1981 ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg
     2041 taacatgtct catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc
     2101 caaaggccaa tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa
     2161 aagattgaga catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag
     2221 ctagaccagg agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc
     2281 ctgtattgat aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa
     2341 agaaggtgta gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga
     2401 cagacaatta ataaaagctt gatgtgttgt gctctttttc gtttttcaca caaataaaga
     2461 caaaaaataa acggtcttct ttttatagaa aaccgtttat tttcgtcttt ttttattttt
     2521 aaagatattt tttcataagg aataaagaat acttcatata aaatatataa aaaagaaccg
     2581 cagcgagtgt cgctacggtt ctcaaatttg ttcttcattt caacataagt ttttcttcaa
     2641 tttgcttata atttacgtgc aaagaatgaa acgactaata ctaaaatgat tgcaccgatc
     2701 aatgatggaa tgatagccat tcctgcaatt gttggtcccc aagttcctag taaagcttgt
     2761 ccaattgctg aacctactaa accagcaatg atgtttgcga tgatccccat tgatgatcct
     2821 ttattcgtga ttgctcctgc aattgctcca atgataccgc cgacgattaa tgcccaaata
     2881 aatcccattt taaattcctc ctaactgact tcttacttct tattgtacac gtggttcgtt
     2941 attttctttg actttttgtg cacctttgcc tacagcagat tttgctttat ctgtttggcg
     3001 tgaagcaaat tgtcctgtgg attttgctgc ttctgttact ttgtcttgta ctgtttcttg
     3061 atctttttgg tattcttctt gtgttttgat gtctgcaaca ttcacattca cttcgataac
     3121 atctaaatgt gtcatattgt ttacttcagt actgatcaat tctttgattt ggttgtaaat
     3181 atcttcgatg tctttaccgt attctacgat cacatctaga tccacagcta cttgtttctt
     3241 acctacttct gtattgatac ctgctgttac gttatctgtg ttgactaatt tttcagccat
     3301 atttgagaaa aatccgccat ctaatgtcaa tagaccatct acatctttca atgcataacc
     3361 gatgattttt tggatgactt tgtcttcata agtcaattca ccattcatca tgcggttttc
     3421 tcctttttca tttgccaaaa ctgatccatt tgttgtattc atgtttttgt tttccatctt
     3481 tgtttcctcc tatgatttaa aaaattgatc aactattcct gactgtttca aataaaaacc
     3541 gattcctgcg ccaattgctg tcatgattat gatcaacaat gtcttgaaaa atccaatcgt
     3601 taaccataaa acagctagca gtagtccaag acccccaaag ataataggga gcttgtatgc
     3661 tgaaaatagt tctttcatta tttggccctc ctattccact cgagagtgct tgtagtctct
     3721 tgtatcttct tgttcttcat aaccgctgta tgtgactgcc actttgaccg tttcttttgt
     3781 tccgagtact ttagttactt cttcttcgat atcttgcatc aatacacctg ttttccctac
     3841 taatgaagaa gttcgtttca agtctccgtt gacatggata tggattcgat ttttcgttga
     3901 acgtatccgt actttaggtg aatgaatgaa ctcttcttcg tgtaaatggg aacgtaccaa
     3961 cccttcgatc gcttttttgt cgattgttag tttgccatct tcccgtttca taacgaaagt
     4021 tcctcttgac tttggataga aaagaacgac tagcaagatg ataatagcaa gaacggcaaa
     4081 tactacagct gcccagaaaa tatattgttg catgtagtaa ttcgttaaag taaagaagcg
     4141 aaatcgttcc aattgttcag gcataatata cggcgcattg atcaacgcaa tcgctagaaa
     4201 tactgaaagt agcagtaaac tcatgataac accgattatt tttaacccct tattcatttt
     4261 ccacctccta taaacttatt tttaccctgt aaataactct accgacaatc acagtataga
     4321 tagcgaacag ttagcatagc atcagtaaat aaagcgctac caaacattgc tggatcataa
     4381 cggcgtttta ttgccagtct gatactgaaa taacaattgc ttacttattc tctatttact
     4441 tttg
//
LOCUS       DAGCXG010000136         4431 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.142, whole genome shotgun sequence.
ACCESSION   DAGCXG010000136 DAGCXG010000000
VERSION     DAGCXG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.142"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(61..957)
                     /locus_tag="MB022_002559"
     CDS             complement(61..957)
                     /locus_tag="MB022_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4467521.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(950..1630)
                     /locus_tag="MB022_002560"
     CDS             complement(950..1630)
                     /locus_tag="MB022_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4467522.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1631..2413)
                     /locus_tag="MB022_002561"
     CDS             complement(1631..2413)
                     /locus_tag="MB022_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467523.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2406..3323)
                     /locus_tag="MB022_002562"
     CDS             complement(2406..3323)
                     /locus_tag="MB022_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4467524.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3487..3810)
                     /locus_tag="MB022_002563"
     CDS             complement(3487..3810)
                     /locus_tag="MB022_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467525.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1303 a          953 c          770 g         1405 t
ORIGIN      
        1 ctctattaaa tatatataag ataatacatt tttctactag ttctcactaa tcttgtataa
       61 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
      121 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
      181 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
      241 ttgcgttcct ggtttgatag gattggccac tgttaatcga atgaatggat cctcttttga
      301 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
      361 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
      421 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
      481 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
      541 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
      601 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
      661 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
      721 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaacc atgtattcag
      781 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
      841 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
      901 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccatt taatcaaatc
      961 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agcttccctc
     1021 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
     1081 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
     1141 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
     1201 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
     1261 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa aagttcttcg atatcaaatg
     1321 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
     1381 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
     1441 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
     1501 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
     1561 gaacctcctc cagtaaggaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
     1621 gcaggaccac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
     1681 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
     1741 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
     1801 ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc catgatataa agaaaagaaa
     1861 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
     1921 tccctaatgt cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
     1981 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaagcatg gctagtcccg
     2041 tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
     2101 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
     2161 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
     2221 ctaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
     2281 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
     2341 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
     2401 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
     2461 ccaactaggt gaataccatc cacaggaact tttgcttcta ccaatgcttg attgatggct
     2521 gctacttggt caattccttc aaaaagctgg ataacacggc tagattgtac aaaataatcc
     2581 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
     2641 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
     2701 tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
     2761 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
     2821 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
     2881 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
     2941 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
     3001 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
     3061 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
     3121 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
     3181 gacttacccg ctccattttc cccaatcaat ccatagatca ttccacgttt gatttcgatt
     3241 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
     3301 accgtttcat ttccaaaatc catacttatc cttctttctt attgatgagt gaagtatagt
     3361 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
     3421 tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt atcaggcagt ttgttatacg
     3481 cttgtatcag tttcctgatt ctttcttttc gaccaaagtc gtttctctgt attcctcttc
     3541 actgaaaatt tttgcttctt tcagatcaag cttgagtaat tttaccagac cggttataag
     3601 gataactata gcaagactcc ctcccagaaa aattagactg tctcgcagac gtggttgcgt
     3661 tgtcttccaa tcaaagagcg caccaaacag tgctaatcca agtggactgg ccaccatggt
     3721 aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc gttggaactt ctcgctgaag
     3781 ccagaccata taaaaagcaa aagaaaacat taatccttaa aaacggactt atgaaaaata
     3841 atacatgccg tttgggattt acaattagct accaaataag aggttatgac aaaagacttt
     3901 tcacaatccc ttcttccgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
     3961 aatatttatc aaacatgaga agcaattttt gtaaattcct tcttatttct tgaagctgac
     4021 cacttctgtc acaacctcag aagcggataa ataatattgt ttgatgatac tttatctttt
     4081 tcccgtttgt ttatagatcg aatgatatta tagaccaaga atcctaccta ttaacatagc
     4141 taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt taggattaag
     4201 gtaactgcgc tcagcattgc ttcgcccagt acgattctcg taactcgtga actcgaacga
     4261 taatcgcaat taacgttttg agaactgtga agctttacgg gctttcttaa gacctggttt
     4321 tttcttcgta tcattgaata agtttgaaaa gccttgattt ataaaggttt ttccgtaaat
     4381 atattttgtt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg t
//
LOCUS       DAGCXG010000137         4391 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGCXG010000137 DAGCXG010000000
VERSION     DAGCXG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.143"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            118..1005
                     /locus_tag="MB022_002564"
     CDS             118..1005
                     /locus_tag="MB022_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4467526.1"
                     /translation="MITIFTPTYNRKKEIKKLYESLLNQTSKGFEWLIVDDGSTDKTK
                     EFFLLLEKQHLPFKIRYIYQENSGKHVAYNTAIKNMKTEWHVVVDSDDILFEKSIEIF
                     EMRIQEIENDMIGLIFPRKNLDSIDISPWIEKKIPMNIPDIKFKFGLNIETCIVINNK
                     YIKKFQFPKFKDEKFMSEESLYYYLADYGKFLPFKDQVYGYRYLEDGWTKNLFRIWEE
                     NPLSTLYSLKMRKKYIQKHVKGLKKTKELIKVSMNINSLEWLNEKSFKKYIKGKSIKQ
                     WFYVPIFFIFKKIRFERKK"
     gene            1007..1873
                     /locus_tag="MB022_002565"
     CDS             1007..1873
                     /locus_tag="MB022_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4467527.1"
                     /translation="MMVSVVIPTFQRNDFLERAINSVFKQNFTDYEILIIDDNVGENV
                     YRINNRNLENKIQNSKIRFIYNEKNLGGAEARNIGIKEAQGKYIAFLDDDDIFLPEKL
                     KKMISVLEEKKGVMAYSFVKSNLGQEWKNVYNGNALFELFKVGSIAATSQWIILRKAL
                     IEVGGFDNTPAKQDSILTCKLLENGNEIICIEEFLSVYFEHEFTRISTSGKTLIGEIN
                     LFNKYKSIKDRFSIQEQKEIELNFNYRIFKYKIKMKKYVSASQNLFNIVRNNPFKGYE
                     LIKKDFIRGGMK"
     gene            1875..3041
                     /locus_tag="MB022_002566"
     CDS             1875..3041
                     /locus_tag="MB022_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBT4467528.1"
                     /translation="MRIALFGLGYVGLANALLLGQHENVKAYDIVEEKIDMLRNRQSP
                     LEDKEVHEFLERDDLNVEFTKDFEEAVLFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALIIRPDATFIIKSTVPVGYTLELKAKFKTENIVFSPEFLREGKALYDNLYPSRIVV
                     GECSDRGQEIADMFERNALDKDVPVLLTHPTEAEAIKLFSNTYLALRVAYFNELDTYA
                     ESRGLNTKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYENVPSNIISAI
                     VDANTTRKDFIADQILAKKPKTVGIYRLTMKANSDNFRYSSIQGVMKRIKAKGIEVVV
                     YEPTLDKEAFYHSRVIKDFDEFKNMSDVIVSNRMEPALAEVKEKVYTRDIFRRD"
     gene            3044..>4391
                     /locus_tag="MB022_002567"
     CDS             3044..>4391
                     /locus_tag="MB022_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBT4467529.1"
                     /translation="MKNLARNFFYQSLFQITKIIIPIITIPIVSNALGPSGIGIYNYT
                     FSIAQYFVLVAGLGVAIYGNREITLILNRNKEELSKVFWEILMFKAMTTIFVLMFYFA
                     LTFFLENKIYLLIQSLTIIAVFFDVSWFFMGIEDFKKTSISNLLVQIVTFVLIVFLIK
                     NEGDALKYTLIQALGLVCSQISVWLFIPKYIHFVKVSIRNSFNHLKGSFEYFIPQIAI
                     MLYTNLNKTLLGFTMGSMAVGYFTNSLQLNTVFITIITTLDLVLLPHMTGFFAKENVT
                     RIVRMMEKTIHLQLYFSIPIMFGMLTVYDKLVPWFFGEKFIYINNVIPYFSALIIIIP
                     LGMSISRQYLMPIGHVKEYNKSVIVGAIINIVANLILLPTIGFFGVVFSNILAECFVT
                     FVRTRSFLKSTDFKFEYRKIGIFLLGGILMCIVTRSLTSNLSSSILTNLIQFAIALP"
BASE COUNT         1625 a          545 c          743 g         1478 t
ORIGIN      
        1 atccatagta tgtatcagtt ttagtgaact ttatcctata gattttaggc ttaatctatt
       61 tcctttattt ttattaagcc gtaaagctaa taatattaag ttattgagag ggcatttatg
      121 ataacaatat ttactcctac ttataataga aaaaaagaaa taaaaaagtt atacgaatca
      181 ttattaaatc aaacaagcaa aggctttgaa tggcttatag tcgatgacgg ttcaactgat
      241 aagactaagg agtttttttt attattagaa aaacaacact taccatttaa aatacggtac
      301 atttaccaag aaaatagtgg caaacatgta gcttataata cagctataaa aaacatgaaa
      361 actgaatggc atgttgtagt tgatagtgat gacattttat ttgaaaaatc aatagaaata
      421 tttgaaatga gaattcaaga aatagaaaat gatatgatag gattaatttt tccgaggaaa
      481 aatttagatt ctattgatat ctctccatgg attgaaaaaa aaatccctat gaatattcct
      541 gatatcaagt ttaagtttgg attaaatatt gaaacttgca ttgttataaa caataaatat
      601 attaaaaaat ttcaatttcc aaaatttaaa gatgaaaaat ttatgagcga agaaagccta
      661 tattattatt tagcagacta tggtaaattt ttgccgttta aagatcaagt atatggatat
      721 cgttatctag aggatggttg gacaaagaat ttatttagaa tttgggaaga aaatccttta
      781 agtacactgt attccctaaa aatgagaaaa aaatatatac agaaacatgt aaaaggatta
      841 aagaaaacca aagaattaat caaagtatct atgaatataa atagtctaga atggttaaat
      901 gaaaaaagtt tcaaaaaata tattaaagga aaaagtataa aacagtggtt ttatgttcca
      961 atttttttta tttttaaaaa gatacgtttt gagaggaaaa aatagtatga tggttagcgt
     1021 agtaatacct acttttcaac gtaatgattt cttggagaga gcaatcaata gtgttttcaa
     1081 acaaaatttt actgattatg aaatcttaat cattgatgat aatgtaggag aaaatgttta
     1141 tagaattaat aatagaaatt tagaaaataa aatccaaaac agcaaaatac gctttatata
     1201 taacgaaaaa aaccttggag gagctgaagc aagaaacatt ggcattaaag aagcacaagg
     1261 taaatatatc gcctttttag atgatgatga tatttttttg cctgaaaaat taaaaaaaat
     1321 gatttcagtt cttgaagaaa aaaaaggagt aatggcttat tcttttgtca aatcaaattt
     1381 aggccaagaa tggaaaaatg tatataatgg aaatgcccta tttgaacttt ttaaggtagg
     1441 atcaattgct gctacatcac aatggataat tcttcgaaaa gcattaatag aggtcggagg
     1501 atttgacaat acaccagcaa aacaagattc tatactaaca tgtaaattac ttgaaaatgg
     1561 taatgagatt atttgtatag aagagtttct atcagtttat tttgaacacg aatttactag
     1621 aataagtaca agcggaaaaa cacttattgg tgaaatcaat ctttttaaca aatataaatc
     1681 aataaaagat agattctcga ttcaggagca aaaagagatc gaattaaatt tcaattacag
     1741 aatatttaag tataaaatta aaatgaaaaa atacgtttct gctagccaaa accttttcaa
     1801 tatagtaaga aacaatcctt ttaagggata tgaattaatt aagaaagatt ttattagagg
     1861 aggaatgaag taagatgcgt atcgcacttt ttggactcgg ctacgttggc ttagctaacg
     1921 ctttattact tggccagcac gaaaatgtta aagcatatga tatcgttgag gaaaagattg
     1981 atatgctaag aaacagacaa tcaccattag aggataaaga agttcacgag tttctcgaac
     2041 gggatgattt aaatgtggaa tttactaaag atttcgagga agctgtactt tttggtgatt
     2101 acttgatcat cgctacaccg acagattacg acgaaaagaa aaattacttt aatacttcca
     2161 cagttgaaga tgttattgaa aaagccctga tcattcgacc agatgcaact tttattatta
     2221 agtcaactgt tcctgtggga tatacattag aattaaaagc aaaatttaaa acagagaata
     2281 ttgttttttc tccagagttt ttaagagaag ggaaggcatt gtatgataac ttgtatcctt
     2341 ctcgaattgt tgtaggagag tgttctgata gagggcaaga aatagcggat atgtttgaac
     2401 ggaacgcttt agataaagat gtgccagttc ttttgacaca tcctactgaa gctgaagcaa
     2461 tcaaattgtt ttcaaacact tatcttgctt tacgcgtagc atattttaat gagttagata
     2521 cttatgccga gtctcgagga ttgaacacaa aacaaatcat tgagggtgta gggttagatc
     2581 ctcgaattgg ctctcactac aataatccct catttggtta cggaggatat tgtttaccta
     2641 aagatacaaa gcagctaaga gcaaactatg aaaatgttcc aagcaatatc atcagtgcaa
     2701 ttgtagacgc aaatacgaca agaaaagatt ttattgcaga ccaaatttta gcgaaaaaac
     2761 ctaaaactgt aggaatctat cgtttaacaa tgaaggcaaa ttctgacaac tttcggtatt
     2821 catctatcca aggtgtaatg aaacgtatca aagcaaaagg gatcgaagtc gtagtttatg
     2881 aaccaacact agataaagaa gcattctatc attcacgggt gatcaaagat tttgacgaat
     2941 tcaagaatat gtcagatgtg attgtatcaa atcgtatgga gccagcttta gctgaagtaa
     3001 aagaaaaagt ttatacgaga gatattttta gacgggatta accatgaaaa atttagctag
     3061 aaattttttt tatcagagct tgtttcaaat tactaaaatt attatcccta taattactat
     3121 tccaatagta tcgaatgcgc tagggccaag tggaatcggt atttataatt atactttttc
     3181 aattgctcaa tattttgttt tagttgctgg attaggtgtc gcaatttatg gaaatagaga
     3241 aattacttta atacttaaca gaaataaaga agaattatca aaagtatttt gggaaatact
     3301 gatgtttaaa gcaatgacaa ctatttttgt tcttatgttt tattttgctt taactttctt
     3361 cttagagaat aaaatttatt tattgataca atccttgacg attatagccg tattttttga
     3421 tgtttcgtgg ttttttatgg ggattgaaga ttttaaaaaa actagtattt ctaatttatt
     3481 ggtacaaatt gttacgtttg ttttaattgt tttcttgatt aaaaatgaag gagatgcatt
     3541 aaaatataca cttattcaag cgttaggttt agtatgttcg caaataagtg tttggctgtt
     3601 tattcctaag tatattcatt ttgtaaaagt aagtataaga aatagcttta atcacttaaa
     3661 gggatcattt gaatatttta taccgcaaat agctattatg ttgtatacaa atttgaataa
     3721 aacgctttta ggatttacta tgggcagcat ggcggttgga tatttcacta actcattaca
     3781 attgaatacc gtttttataa ccattattac tacgttagac ttagtgttat tacctcatat
     3841 gactggcttt tttgcaaaag aaaatgtgac tagaatcgtt cgaatgatgg agaaaactat
     3901 acatttacaa ttatactttt caatacctat tatgtttggc atgctaactg tttatgacaa
     3961 attagtgccg tggttttttg gagaaaaatt tatatacatt aataatgtta ttccttattt
     4021 ttcagctcta ataataatta ttccgttagg tatgtcaatt tccagacaat atttaatgcc
     4081 gatagggcac gtcaaggaat ataacaaatc agtaattgtt ggggccatca tcaatatcgt
     4141 agccaattta atattgttac caactattgg tttttttggt gtagtttttt cgaatatttt
     4201 agcagaatgc tttgtaactt ttgtaaggac aagatctttc ttaaagagta cagattttaa
     4261 atttgaatat cgtaaaattg gaatcttttt gctaggcgga atacttatgt gcatagtaac
     4321 acgttcttta acaagtaatt tatcaagtag tattttgact aatttgatac agtttgctat
     4381 tgctttgcct a
//
LOCUS       DAGCXG010000138         4383 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGCXG010000138 DAGCXG010000000
VERSION     DAGCXG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.144"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(83..1051)
                     /locus_tag="MB022_002568"
     CDS             complement(83..1051)
                     /locus_tag="MB022_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4467530.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="MB022_002569"
     CDS             complement(1498..2904)
                     /locus_tag="MB022_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBT4467531.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="MB022_002570"
     CDS             complement(3126..3488)
                     /locus_tag="MB022_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467532.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3839)
                     /locus_tag="MB022_002571"
     CDS             complement(3492..3839)
                     /locus_tag="MB022_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467533.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLDRMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQI
                     GVIYRNKSKGKEL"
     gene            complement(3818..4237)
                     /locus_tag="MB022_002572"
     CDS             complement(3818..4237)
                     /locus_tag="MB022_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467534.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4230..4370)
                     /locus_tag="MB022_002573"
     CDS             complement(4230..4370)
                     /locus_tag="MB022_002573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467535.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1240 a          993 c          612 g         1538 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa ttgacgataa ccaccgctca aaatcatcaa aagattcaaa tccaaaatgc
     3361 ttccgaagtg ccttacccat ttcagcattt tccttctcaa tcaatcgttt tttctgttgg
     3421 cgaagttttg cctgttcttt tttcaatttt tcttcttgtg ctttaatatc tgctaaagtc
     3481 attcccaaga tttacagctc cttgcctttt gatttatttc gataaataac accaatttga
     3541 tttaattgca taacataagt taaaatattc agccaaaatt ccttttcaga ttcatcttct
     3601 gtttcggcta ttttaccgta aacaaattta tcagtaatca aagaagaatt tgctaggcct
     3661 acattgattg tagaattgag aacgtccatc attctatcaa gcatttcact ataattatct
     3721 tcatccggca aattcaaata atcagctaat cgttcataca tcaattcaat tctatcgact
     3781 gtttcttctc tcaaatcaat tttatccatg tcacacctca cttccagaat tgccaccatt
     3841 ttcgattttt taattcttga ttttctagta acaactgatc gtttttttgt tgctgaagca
     3901 aagtcaattt ttgttggtca cgaatcattt ccgttaattc agaaatatgc ctatcttttt
     3961 ccgctaattg ttcacgcaaa aattcatttt ctttttctaa cgggaaatca ttgcctgcat
     4021 cattgaccgg taaatcacca gtcaattgac caccttttga ccgataaatt gaccgcacaa
     4081 caccaatcaa ttccttgtct aacatgataa ccccattatc tttataccga gcaaggtcat
     4141 aacctgcatt gaccagttga ttgcgcactg tatttttaga tatacccaac tcatcagcaa
     4201 ggttttttat ggtcattttt tctggcaaat cagtcattat cttctccgtt actttcagct
     4261 accccataaa catatttaac agcatgctta gggataatga ttggggaatc aattttatta
     4321 tttttcaaaa tcaactcata ctgcgttaca tcaagtaact taccggacat ttttttgcta
     4381 tcc
//
LOCUS       DAGCXG010000139         4169 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGCXG010000139 DAGCXG010000000
VERSION     DAGCXG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.145"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..266
                     /locus_tag="MB022_002574"
     CDS             <1..266
                     /locus_tag="MB022_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467536.1"
                     /translation="KVYDNPISCALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIK
                     LKKGDRITITQKASGLIFSLFATKPFYYPSHCEVNLIGSEKNG"
     gene            259..654
                     /locus_tag="MB022_002575"
     CDS             259..654
                     /locus_tag="MB022_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBT4467537.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            656..1033
                     /locus_tag="MB022_002576"
     CDS             656..1033
                     /locus_tag="MB022_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467538.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1034..1642
                     /locus_tag="MB022_002577"
     CDS             1034..1642
                     /locus_tag="MB022_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4467539.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            1642..1983
                     /locus_tag="MB022_002578"
     CDS             1642..1983
                     /locus_tag="MB022_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467540.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2225..>4169
                     /locus_tag="MB022_002579"
     CDS             2225..>4169
                     /locus_tag="MB022_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBT4467541.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWL"
BASE COUNT         1474 a          540 c          861 g         1294 t
ORIGIN      
        1 aaaaagtata tgataatcct atttcttgcg ctctttcaca aggtcaaatc gatggactag
       61 cagtcataga agatggagag atggtaaatg tttcaactga cacatataaa ttatttgttc
      121 atcctaagat taaactaaaa aaaggagatc gaataacaat aactcaaaag gccagtggct
      181 taattttttc tctatttgct actaagcctt tttactatcc tagtcattgt gaagtgaatt
      241 tgataggaag tgagaaaaat gggtaatctc aaatatgaat caaatgcaga aaagatcatt
      301 gagaatttta aaaatatgac tgtaattgct caaaaagaag gaatatcctt cgttaacgat
      361 tcaatgaata aagtcgttag tttagctaaa ccattaactc ctgtaaaatc aggtaattta
      421 aggcgaggat atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat tgttggagca
      481 gttataaaca atgaacatta ttttaaatat gtagaagaag gtcataggac taaaaatggt
      541 ggctttgtca aagggaaatt catgttaact cgtgcaacga atcttgcaaa tatgtcttat
      601 attcctcgaa gatttaaaca aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa
      661 agatgaaatc attgctgcaa tcagtagcaa attaaaagaa atctatcctg atgggacaat
      721 atatcttgat tcagttatgc agtcaactaa agatttttat ttcgttttat cagtaatgga
      781 atctggaact gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa ttgatattgc
      841 attgattgat aataaaccta atagaaattt aattaatgaa ttagtctcac gttgtgggac
      901 tttttttaat acgattacaa tcgatgagca aatattattt ccaaaagcct atttacctga
      961 tgaagcagat ggtgttcaac atattcgttt tacattagaa tttccacaat atattgaatg
     1021 gagtgaaaga taaatgggag aaaaaagaag taaaaccgga attatttctg tcgaaaaacc
     1081 tacttggttt cctttggaag atgagacagg gaattttccg gtatatggta ctgcgttgcc
     1141 aatgggaaca gcagtaagta tcaaaccaac agctaattat gaaacaacac aagattatgg
     1201 ggattcagtg gttcaagatc aatttacagc ttttggtggt gcagaagttg aactggaagc
     1261 aaatggatat actcataaag ttttatctac aattactgga gggaaaattg ttaaaggtgg
     1321 tgccttgcgt tctggagaag atattgcgca agatggtgcg tttgcatata gacgtaaaaa
     1381 atcgaatggg aaataccgtt atacggtctt ttataaagga cagtttgctc tagattcaga
     1441 tgaaacatca acgattgaag gaagtaaagt aagttttact catccaactt ggaagggttc
     1501 atttgtagat gtaccaggac ttggatatat gtattcagtt gatgaagatg atgaaggcgt
     1561 ggataaagca atgatcggaa attggtttac aaaagtagca attccaattg aagaagcaga
     1621 actttcagga ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa
     1681 gatgaagaag gtaaatatga gcaagtacaa tttaaatctg ctgaattttt accaggaaca
     1741 gttgtagaag atgctgcagg agttatggaa gaaatgcaaa cagcgactga taaacaatca
     1801 gttaaaaaag ctttaagtcg tgcttattca tttattgcag atactttatt tgagggacaa
     1861 tttactggtg aagattattg caaaggaatt gatgctcgtg agattgcctc attgacagga
     1921 aaattattga agtctgttac tgcaggtttt gatgaaactt atacagagac gaaaaaaaag
     1981 taagtgaggc gctcaagtca ccttcattta agtattcgat tacttaccga gaattagata
     2041 taaagacgca attactcgaa gcaggttgga cgttaccaga aattgaacgt actgacttgg
     2101 atgagttaat gcgtcttttt gcttttagag atgcggtaaa agagcatgaa gatgttgagt
     2161 actatgataa tttcactcaa ttttaggagg tgatactttg aataacgatg acctaattct
     2221 gaagatgata ctagatgaat ctggttttac tgccggtatg aataatgctg tcaaaaagtt
     2281 gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt cattaggtag
     2341 tatttggaaa atatttgctg gaagttttct agctagcgga gtaactagaa tcgtaggagc
     2401 tggctttgat ctaatcaagg gttccataag tggagcggtt gatcgagtag atacgatgaa
     2461 taacgctcta cgaaatttcc aaaatatggg atttagtaat tcagaaatta tgaagaatat
     2521 cggaaagaat gggcttttat ctcaaggtat tcaaggactt cctactgctt tgaatgatgc
     2581 gataagtcat gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt caactcaaat
     2641 atttaaagct ttaaatgatg gaattcttgg tttcggtggt tcaactgatc aagttaatga
     2701 agctgttatt caattatctc aaagtttctc aaacggaaaa gtagacgcac aaacttggaa
     2761 ctcaatgatc aacgctcaac ttggacctac tctttctgct attgctaaaa agatgggaat
     2821 tacaatggga gatctgaaag aaggtttgtc tcaaggtaag atttctgttg aagaattcca
     2881 aaatcaatta atagaaatgg ataccaaagg tggcggagga cttaaatcat taagtcaaat
     2941 cgctaaagat tctactaagg gaattaaaac ctctatacaa aatgcaaaaa cagctgttac
     3001 acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca ttagtagatt cagatttagg
     3061 tggatttaag ggaattattg ataaagtggg cagttcaatg gaatcgttct taaaagtaat
     3121 tgctgcaaat attcctacag cagtatcatt tttaagtaat ttatttaacg aaattcaaaa
     3181 atttggttct gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg
     3241 agcgttcatg tttcaactta aaggtatacc agcaataata aaaagcttca ataattttaa
     3301 gaatgccata attggcgttg gaaattcatt aaagattatg ggggcaatag ctgctgctaa
     3361 ttcatttgtt ttaattattg gtgcagtcgt tggtgcaata gctgttttcg gttactttat
     3421 ggcaaccaat gaagagttta gaaacaaggt tatatctatt tggaatgatg tgaaagattc
     3481 cgtaattggt gtattaaaaa atataaagga ctggggaatt gatacttgga attctgctaa
     3541 agaaatggca tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa caaaggaatg
     3601 gttttctaac acctggaaag gtattaagga tggggccaca ggtttatttg ataaaacagt
     3661 agagacttca agaaatgcag tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg
     3721 gttttctgat acttggcaaa gtataaaaga ttcagccaga gaaaaatgga atgaaattaa
     3781 aggttctatt atgtcggttg ctggtccatt gattacaggt ataaagaatg catttttaca
     3841 tgttactttt tatctagaga ccttatggaa taatttagtt gagattggta aaaacgtctt
     3901 tgagatttta aaaaatgtta tacttgcgcc ggttttgttt attacctcca tgatttctgg
     3961 tggatgggaa gaaacaaaaa ataatatgat tgctgtttgg aataatatta aagaaagtgc
     4021 cataaatatt tgggaatcta taaagaatat atttgtaagt tattttacaa atatttattt
     4081 tgctgcactc aatatttgga cagggttcaa gcttacattg ataaatattt ggaatgaagt
     4141 ggtaaatcaa gctaaatcga tttggttaa
//
LOCUS       DAGCXG010000140         4139 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGCXG010000140 DAGCXG010000000
VERSION     DAGCXG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.146"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            47..1348
                     /locus_tag="MB022_002580"
     CDS             47..1348
                     /locus_tag="MB022_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4467542.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="MB022_002581"
     CDS             1375..1635
                     /locus_tag="MB022_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4467543.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="MB022_002582"
     CDS             complement(2634..3869)
                     /locus_tag="MB022_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBT4467544.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1194 a          666 c          862 g         1417 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa taaaataat
//
LOCUS       DAGCXG010000141         4114 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGCXG010000141 DAGCXG010000000
VERSION     DAGCXG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.147"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            135..914
                     /locus_tag="MB022_002583"
     CDS             135..914
                     /locus_tag="MB022_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4467545.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            907..1224
                     /locus_tag="MB022_002584"
     CDS             907..1224
                     /locus_tag="MB022_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4467546.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1468..3078
                     /locus_tag="MB022_002585"
     CDS             1468..3078
                     /locus_tag="MB022_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4467547.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3235..4083
                     /locus_tag="MB022_002586"
     CDS             3235..4083
                     /locus_tag="MB022_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4467548.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         1209 a          712 c          836 g         1357 t
ORIGIN      
        1 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
       61 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
      121 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
      181 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
      241 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
      301 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
      361 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
      421 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
      481 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
      541 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
      601 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
      661 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
      721 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
      781 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
      841 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
      901 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
      961 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
     1021 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
     1081 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
     1141 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
     1201 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
     1261 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
     1321 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
     1381 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
     1441 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
     1501 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
     1561 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
     1621 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
     1681 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
     1741 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
     1801 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
     1861 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
     1921 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
     1981 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
     2041 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
     2101 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
     2161 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
     2221 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
     2281 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
     2341 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
     2401 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
     2461 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
     2521 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
     2581 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
     2641 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
     2701 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
     2761 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
     2821 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
     2881 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
     2941 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
     3001 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
     3061 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
     3121 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
     3181 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
     3241 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
     3301 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
     3361 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
     3421 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
     3481 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
     3541 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
     3601 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
     3661 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
     3721 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
     3781 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
     3841 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
     3901 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
     3961 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
     4021 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
     4081 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAGCXG010000142         4044 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGCXG010000142 DAGCXG010000000
VERSION     DAGCXG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.148"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(153..1658)
                     /locus_tag="MB022_002587"
     CDS             complement(153..1658)
                     /locus_tag="MB022_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4467549.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="MB022_002588"
     CDS             complement(1655..2341)
                     /locus_tag="MB022_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4467550.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="MB022_002589"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="MB022_002589"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBT4467551.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1115 a          856 c          691 g         1382 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat attt
//
LOCUS       DAGCXG010000143         4024 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGCXG010000143 DAGCXG010000000
VERSION     DAGCXG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.149"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..143)
                     /locus_tag="MB022_002590"
     CDS             complement(<1..143)
                     /locus_tag="MB022_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467552.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQS"
     gene            complement(146..502)
                     /locus_tag="MB022_002591"
     CDS             complement(146..502)
                     /locus_tag="MB022_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467553.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(800..1774)
                     /gene="sgrA"
                     /locus_tag="MB022_002592"
     CDS             complement(800..1774)
                     /gene="sgrA"
                     /locus_tag="MB022_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBT4467554.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1970..2809)
                     /locus_tag="MB022_002593"
     CDS             complement(1970..2809)
                     /locus_tag="MB022_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4467555.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2994..3881
                     /locus_tag="MB022_002594"
     CDS             2994..3881
                     /locus_tag="MB022_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4467556.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1344 a          807 c          594 g         1279 t
ORIGIN      
        1 gactgccaat acatcaagtg aaaaaagtat cggatacggt attcttctcc ttctatcgga
       61 ttaccttttt tcaagctgca agataaatga tagtttttca aaatagtatt aagttctctt
      121 aattttcttt gaatcgtacg caaacttata taattttttg actaagattt tctaaggatt
      181 gataagtacc tttgtaaagt gaatccagta aaatataacc aatcgttccc tctaaaagta
      241 tagaataaac tttactcagt aaaaactgag aagaaaactc gacttttatc atcttgtttt
      301 ctgaaagaaa agataattta tattctggat acctttcgca aaacgattct gcttgttgaa
      361 tcaccgaatt catcttagcc agggtgtagt ttagatctaa agcgatatcc ttcaccacat
      421 ataatttctc gtctctttca taacaatagt tcaaaaattg atatacttgc ctttcattga
      481 tatcaataaa ttcttcaaac aaagtacata ctccttttta tctattattt agacttattc
      541 cgacagctac atgaataacg tacaaataga ttcgttactg atttttcaga gatactacca
      601 tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc catttgtgtt cctgttattt
      661 cctgtttctc aaatatgtta gtaaacaaaa atagatctgg caaagctaca acttcgccag
      721 gcaatacagc actctcgaat gaaatattca ctcaaaaatc tatcgtttta ttcaatttta
      781 gatctgttgc ttttattctt tatttcactc gatggaagag agaacgaaaa tagcttgctc
      841 ctacaatcaa aatgagtaac acacccatcc atgtaattag cttgttcaag gttctactac
      901 cagtttctgg taaatcgctt tttctattat ctgtttcttt cgctttttct tgatgatcaa
      961 ttccacgaga aacaaaatca ggctgtttta gcacactttt taggttgctc cctgcatttc
     1021 tcttttgttc cttagttggt atgataggta ccaaatcaag ttcattattt acttttcctg
     1081 tgctgctttc gctgctcgtg cttcctgtgc tactctcact actcatgctt cctgtgctac
     1141 tttcactact cgtactctcc gtactgcttt cgctgctcgt gcttcctgtg ctactttcac
     1201 tactcgtact ctccgtactg ctttcgctgc tcgtgttttc tgtgctactt tcactgctcg
     1261 tactctctgt actgctttca ccgctcgtgt tttctgtact gctttcacta cttgtgctat
     1321 ccgtactgct tggcggtcca acaactgtcc aactgcttgg tttcaaattc tccgtgatcg
     1381 tcatctgtcc atcataaaaa acaatcaaat caactttatc tttactcttg atctcatcaa
     1441 tcgatagttc tgcagaaatc attattgtat actcaatacc agcggtccat tgtacgtctt
     1501 ttaaataaac ttccccaata actttttctg aacttccatt accatcccaa ccatatgtta
     1561 gaacagcatg tacatcacta gcattcacgc ttttgtttgg tttgactgga atagtaatga
     1621 cttgttgtgt catgtcataa acaatccgat cagttttcac agtaaatgaa agattctcat
     1681 ttgcccgttc atttgcagaa gtactgattc caatcattgc tgcaattata ctcagcccaa
     1741 gaataacaaa taatacggtc gctgttttct tcatttctat cactctccat ataattacct
     1801 tctatttgat aaaggaatta tataaaatga aaatgatgag aacacgacag tcagttgtga
     1861 aaatctaaca aaagatgtcg tgtattcgct tgaatttgtt tactttttcg tttaatcaat
     1921 gacagactta aaaaagcaag accgatatca gccttgcttt cttcatcttt tattgataaa
     1981 cagcagtgta tttcttcatc cgctttgttt tatatgcata gagtaacaaa ccaacaatca
     2041 agactaacaa gaataatgta gttgatatag atgcttcagc cccaaagttt cccccagtaa
     2101 ttattgtatt ttgcgattgg atcagagtgg agaaaaaacc ttgttcttgc gttccgctga
     2161 ctggtatacc caaaatagct ccttgtgtcc aattccaagt aatatgagca ccaatagccg
     2221 taaataaact tcctgtactg tagataagtg cgctgaaaaa gcaacctgct aaaaagatat
     2281 tgaaaactgc cagaaatgtc atgttaggat tcaatccgtg caaaagagca aataatgcag
     2341 cagatccaat cattgctatt aacggaccaa atctaagcat ctgatgctga aaaaagccac
     2401 ggaataaaag ctcttctgat gcccccactg caataaataa aataaaactc gttatcagcg
     2461 gcacaatgtt tgcgtttcct gaatctaacc gatacgcccc cgtcaaatat aatggactag
     2521 taataagcag aaacactcca attccaacaa cgagacctcc cgctaaatac ccccagtctt
     2581 ttcctcgaac agaaacacca taatcttcta ttctttttcc ttcgacctta ctagcaagaa
     2641 ataaagctag taaaaaaaca gctaaatgtc cgaccgtacc ggcaattgct ccaatccatt
     2701 cactatttcc aataaacata tccgcaagtg cattattgac aagaattcct aaaacaaaac
     2761 caactaagac tattaaaaca gatccaatca accgaataat cctgatcatc tttcatccct
     2821 cctttatatt aagtttaaca acccttttct ttcttgtctc gtgaaaacta tgtaaatgtt
     2881 ctccatcata gagagaatag atttttacga actaagaata tctacagcaa ccttacgaaa
     2941 atgaaagact gtctccctaa ttcttctgtt agactgtttt taataaggaa aggatgggaa
     3001 attttttgaa aaaacaacta ctaattatgt gcctttcagc atgtgcaatt ttactattct
     3061 caggttgtag caagtccaat gaaacgattg caacgtttaa aggtggaaca cttacagttc
     3121 aggattttta tgataaccca aaagttaaat caaataatca aacactatta aaaaaaatga
     3181 ttgtcttcaa agcatttgat gatgtatacg gaaaagagat ttcttcaaag gaagtaacaa
     3241 aagaatataa tgaacaaatc aaaaaactcg gtccaaacta caaggaacaa ttaaaagcag
     3301 ttggccaaac agaagagaca tataagaaac tctttaaaca gatgctggct ttccaatacg
     3361 gtttgaaagc caatgtaaaa ttaacagata aagatctaga cactgcttgg aaagaattct
     3421 atccagaagt tagtacacag atcattctat tttcgactaa agaagaagca gataaagcaa
     3481 aaaaagaagc aaatgaaggg gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata
     3541 aaacattaaa ggaaactgat ggaaaaatga attttgattc aaccaatcca gagattccta
     3601 ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga agtttctgat atcattcctg
     3661 taacagatcc aacgacttat cagcaaagct attatctagt aaaaatggta aaaaaacagg
     3721 ataaaggttc taataaagac aaatacaaat ctgagctaga aaaaattgct accgaagcaa
     3781 aaactcttga tacggaattc atggataaaa caatccgtaa agtgatgaaa aaagacaatg
     3841 taactattaa agatccttat gtaaaaaata tattcaaata aacaaatttg acccaattgg
     3901 ggatgtggca aaatttgaga agtgattttt ggaaattcct tcttattttt gaggcaaacc
     3961 acttttgcca caacttcatc ggggcctttt tttatttgta ccaaaacaaa tcactggatt
     4021 tgtt
//
LOCUS       DAGCXG010000144         3683 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGCXG010000144 DAGCXG010000000
VERSION     DAGCXG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.150"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            53..505
                     /locus_tag="MB022_002595"
     CDS             53..505
                     /locus_tag="MB022_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467557.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            519..1208
                     /locus_tag="MB022_002596"
     CDS             519..1208
                     /locus_tag="MB022_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467558.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1333..>3683
                     /locus_tag="MB022_002597"
     CDS             1333..>3683
                     /locus_tag="MB022_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBT4467559.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1254 a          478 c          769 g         1182 t
ORIGIN      
        1 atcttatcgt agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa
       61 aacgatacta agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa
      121 tttagattct gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa
      181 taaaaaaatg agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga
      241 atttggtgat ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga
      301 taaaattgtt gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt
      361 tagttataaa caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc
      421 gattaaggat ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga
      481 aactaattca gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt
      541 ctgttgggct acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag
      601 ttatttcaga tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata
      661 aagtacttgc attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg
      721 ataatccaga tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag
      781 tgacagaagc aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa
      841 ttatgcctcg aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag
      901 caggaggagg aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta
      961 tgggtgtaaa accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg
     1021 atggaagagt aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa
     1081 tggcattgta tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa
     1141 tgaatatagg tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa
     1201 atatttaata aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg
     1261 acaaacacta aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag
     1321 caggggattt ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac
     1381 agtaatgtta cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc
     1441 tatggactta ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt
     1501 ggacctacca ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta
     1561 cctaaaaata ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt
     1621 ggcttcgatg gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat
     1681 gcagggttta atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta
     1741 tttgatatga gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag
     1801 caatggttaa atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat
     1861 caaagaaatt caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc
     1921 agtgaagcaa cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa
     1981 ggtaactcgg gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt
     2041 gaagacggta cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc
     2101 cgaaaattta tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga
     2161 ctattgtcta gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa
     2221 ttaactagat ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat
     2281 cttactggta ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa
     2341 ttgaataata ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca
     2401 gcattagctt attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca
     2461 gcaaaaaatc taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg
     2521 ttaggggaag aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg
     2581 tcatatagcg ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc
     2641 aataacgggt atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat
     2701 ttaggttttc ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga
     2761 agtggcaacg gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt
     2821 aatgagttat caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt
     2881 ataaatcaga tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg
     2941 aaccctcatt tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt
     3001 tcttctcctg taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact
     3061 aatagcgagg gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg
     3121 cctcacttaa ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt
     3181 gtttctgact ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa
     3241 ttaggaatac aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca
     3301 tttagtctag aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa
     3361 agaaattctg attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct
     3421 attgatagat ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca
     3481 gtaacaaata gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt
     3541 tcatttttca ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat
     3601 tttattggag atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa
     3661 gaaaatagaa gtaaacttaa tta
//
LOCUS       DAGCXG010000145         3549 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGCXG010000145 DAGCXG010000000
VERSION     DAGCXG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.151"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(94..1224)
                     /locus_tag="MB022_002598"
     CDS             complement(94..1224)
                     /locus_tag="MB022_002598"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBT4467560.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1284..1511)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1873..3075)
                     /locus_tag="MB022_002599"
     CDS             complement(1873..3075)
                     /locus_tag="MB022_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4467561.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3135..3338)
                     /locus_tag="MB022_002600"
     CDS             complement(3135..3338)
                     /locus_tag="MB022_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBT4467562.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
BASE COUNT         1019 a          721 c          547 g         1262 t
ORIGIN      
        1 aataatataa aaggtaaata aaaaagagtg gatttctcca ctccatgaaa agccgacttt
       61 ttgggacgct tttttttgcc aagcctcaca tttttatagc caaacttctt ttgcgacttg
      121 ctgcaataat tggattttct tccattgttc ttcttctgtt aaatggtttc cttcttctgt
      181 tgaagcaaac ccacattgag gactgatact tagattttcc aatggtacaa attgttgtgc
      241 ttcttttatt cgttctttta ttttctccgt atcttccaaa tctggaaact tagaagtaac
      301 taatcctaaa acaactttac tttctttgtt actccaatgt cttaaaggag taaaatctcc
      361 tgctcggtca gaatcgtatt ccaaaaagaa accatcataa tttgttgaaa acaactcttc
      421 tgcaatgggt tcatatccac cggaaaacag ccaagaagaa gcgtgattcc cgcggcagat
      481 atgtgttgtg atcgtcaaat cttctggttt gtcttccagt gcgttattga taatctctgt
      541 acaaatatgg gccatttctt tcggatcaat tcctttttga agaagtgctt ctcttttagt
      601 ttgatcagct agaaacgccc aatttgtatc atcgaattgc aggtaacggc aacctttttc
      661 ataaaaagca tgaatcgctg ctttgtaagt agaggacaag tcctcataaa aatcagatag
      721 atcaggataa atctccttga tttttgaaga accatcatta gataggatct cttgtcgtaa
      781 aatcatcgtg ggactaggga tcgttgcttt tgcaacaaaa gtatcatctg tttccactac
      841 ttctttcaga aaagcaaaat cttttaaaaa tgggtgattt ggattgaaag ataatttatc
      901 tactacccga atattataag ctggtgctgc ttttccgtgg aatgtttgat tatatccagt
      961 ttctggaaca tagccttcga taccatttag ttcttctaaa aaatcaagat gccaaaatcc
     1021 acgccgaaat tcaccatctg taattccttt gatgccgact gctttttggt cttccactaa
     1081 tttttttatt tcctggtctt cgattgcacg aagttcttct tttgaaagta agccttcttg
     1141 aacttttttt cttgcttctt tgatcgattg cgggcgtaag aaacttccga catgatctac
     1201 tgtaaatggt ctatttttgc tcatttcact cactcttttc tttgtttttt gtaaaaaaaa
     1261 atccttttac tagcaaatac tagtaaaagg acgattatac tcgtggtacc accttttttc
     1321 agaaaaaaca ttctttctct cagcctgaca ataaaatcag tggtcttggt aacgaaagac
     1381 tgttcgtttt tgcctacctt tcgacaaaaa atcctctaag gccattttcg acagtcaact
     1441 tctagctttt tacaccaacc aaagcctctc ttgaaccatt ttctgtctac tttccttctc
     1501 atcggatact attagaataa aattagaata gcatgtttga aagaaaagtc aatagggaat
     1561 aagaacaaac aaaaactaat ttgttattct tagtaatttg tttgtggaat acaataaagc
     1621 ttttctaata tgataattgt tcagtcgtcc tttcatctta gatcacttcc ttaaaatgat
     1681 catacccgtt tcaaccacat aaaaaaggac ttaattttga aaaatttccc atcaaaacta
     1741 agtccttttt attatagtta tgcaaatttg cttattttct cgctatttcc gatacatctt
     1801 gaacaggagc aattcgactg aaaacactgg tgcaaaagca tttatttgtc ttatggtaga
     1861 accgtggaaa actcagactg gtaataaatt gtcatttttt gaaagaatag cgccctcttg
     1921 aacattatca atcgcactta ccacttgttg atctgacttt gcatataagt gaccgtaagt
     1981 tcctaaagtg atttgaatat tcgcatggcc caatcgttct tgaatagata gcggttgaat
     2041 attctgctca attagaaatg caacatgaga atgtcgtagc gcgtgaagct taatcggttt
     2101 gacacccgct tttttagcag cttcttttaa ccagttcgca aaagaataag gtggcgtaaa
     2161 tgttccgtcc aactgagcaa catattcaaa atttccaata cgtttttgca agtcatacca
     2221 ctgtctgagt tttaaggaaa gcttcttccc caacccaatc gttcgataag cacatttagt
     2281 tttgggagtc gaaaagtacc aatcatcttt tgtattataa atcaatgtct tctcaatcgt
     2341 tatcgtgttt ttctcaaaat ctacattcga ccaagacaaa gcttcaagtt ctccaatccg
     2401 aatgccagta taaaagagca tttcatacac aatcttccga tggtgggttt gaatcttatc
     2461 atccttcaat tcattcatca ctttttgaaa ctcactgtgt tcccaaaact caactttctt
     2521 attctcagaa ggaagcgaag aaacatcttc aacgggattt ttagcaatca ccccctcttt
     2581 aacagcccga tccaatacaa tttttaggtg ccctcgaatc cgattgatat aagattttga
     2641 taacgtttcg ccattaactt ttttccgtga attacgtgac tctgtctggc ttagttcaaa
     2701 taaccaatcc tcaatatcgt gacttgtaat atctcgaaca cgaaaatgat agaaaaactg
     2761 catacgcttt tcgaaaatga attgcgcatt ctcatatgtt tgacttttga cctgtctctc
     2821 gtaccatttt acaaacctat ccttgtaaaa ctcttcgaaa gtcatttgat tggtagaaca
     2881 cagttgatta ggaattgtct tgttaaaatc atgtgtttcc atgtacttgt cataagcaat
     2941 tttggcatct ttttgtgaag cataacctcg tttagtcttc tgaacctttc ttccattttc
     3001 atctatacct agatacgctc ggaagaacca tttttttgtc tttttatctt ggtaaatatt
     3061 tttaattttt ggcatcgttc cattcctccc tgtacttgag aaaagctact ttaatagtac
     3121 cgcgcttgta ctgtttattc cgcaacttga atgtgaaaaa gatgttcaat tactcgggat
     3181 ggcaccacac caacttggcg attattataa aaatcaatac cttcaatcct tgcaagatat
     3241 gccttgctct ctttaatcat tcgtgcagct tgactaggtt taaagcccat ctctatcaaa
     3301 attgcacgat ttaccatgaa atcacatttt gtttccattc tctcacctcc tgattcttta
     3361 ctccttttaa aataatcctc tttataatct attgtttctc attcgatggt tgtgttatac
     3421 tcgtcttgtc gaggcggagt acgttgcaca acttgttgtg taagcagtac tttgcttttt
     3481 tagtagcttg ttttctcatt tgatatactt tgtaattttc gttcataata ctcaacctcc
     3541 tcctgataa
//
LOCUS       DAGCXG010000146         3500 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGCXG010000146 DAGCXG010000000
VERSION     DAGCXG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.152"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..974)
                     /locus_tag="MB022_002601"
     CDS             complement(<1..974)
                     /locus_tag="MB022_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4467563.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKL"
     gene            complement(1323..1649)
                     /locus_tag="MB022_002602"
     CDS             complement(1323..1649)
                     /locus_tag="MB022_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467564.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1636..2439)
                     /locus_tag="MB022_002603"
     CDS             complement(1636..2439)
                     /locus_tag="MB022_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4467565.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            2755..>3500
                     /locus_tag="MB022_002604"
     CDS             2755..>3500
                     /locus_tag="MB022_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBT4467566.1"
                     /translation="MNDFTTEIVQTLVTKGGLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT         1080 a          591 c          527 g         1302 t
ORIGIN      
        1 agctttgccg caatagcttt ttcgacaaac caatttttta cataaacgaa aattagattc
       61 tgcaaattgt ttactttttc agttttttgt ttttgataag ctttcagtaa agtcatatat
      121 aatcctttgt cagcatcaat tccataagat tccagttctt cataaacgac aatagtttgc
      181 tctaattctt cagctttttt ctttgcatta tgaatagttt ggacggtttg ttgagcttca
      241 ctgtaacttg aagaaaatgt ttttatcaag tttaatacat tttctggtac aaatgtattt
      301 atactgtcct cttgcatgat gtcgacgaag ttatctagca aaacttgatt ttgtaactgg
      361 tcagtttcag tgtctattat gtgtctatta gtgtctatag aattagaata gaatagattg
      421 tctgcgattt tcgcaattac ggttgagtcc ttgatatgac tggattttgc cgtatttgcg
      481 attttcgcaa ttacggtaga atcattgatt tttcgcggtt ttgccgtatt tgcgattttc
      541 gcaattacgg tggaatcagt attgtcttcc tcttccatga ttttaaagac gtcttgattc
      601 gtaacttctg gcttgcctaa gtacaataaa ttaggcatag attcaatctt accatcacgt
      661 ttatttactc ttcctctctt ctgtttcaga agacctgctt tttctaattc ttttttgatc
      721 ttagatactt taccttcgcg gcattgtaac aatttcatta attcttcaac tgtgaatatg
      781 aagaatatat tatcatcaga atctacccag ttatttttga ctgaataatc aaatcgatct
      841 ttaagtagca tataagcaat ttttgcatca tttgaaagat ccatgtattt ttcgttagta
      901 aaaaatacct taggcatttg ataatatttt tcacgataaa tttcttttga tttgtaataa
      961 ttaaaatcat tcatagccaa tcctctttct gtaggatagc gttttgttca aaactatgtt
     1021 acaatgaatt aaacaaatat atctatccac tatatatttt tctactgctg ataagtcgcc
     1081 aaactttgtt aaagcagtag tcttttttta tcttttttta gtttacctta aaaaataaat
     1141 taaagcaaac cgctctattt cagggtttaa gctcatggta actatatcac gatattacaa
     1201 taacattata tcacgatatc gtaatattac aatatgaaga taagcgactt atcagctttg
     1261 atactactga tttttataaa aaaaatatca cgatattatg atatcgtgat attgaatgta
     1321 aattaaattt tattttgttt ctttgctagt ttatatcctt caatcaagta ttcttcaggt
     1381 atggatttta atagcgcatc ttctataacg tctggtttac ttttgtctga ttcagcaata
     1441 gtcgtccaat atttgagtgc tgacattaga attggattta tacgaatgct atctgctgtt
     1501 cttacactac ttccttgatc ttttatgact ttacttatat ttttcttggt taattcgatt
     1561 tcttctactt cttttggttt gtagttcttt ttatttttat ttcctagtag tctacttact
     1621 gcagcttgac tgcgattatc ttctgccatc aaaatcacca acttctttta aatattcttc
     1681 tgtaaatgct ttatagtcat cttcagctgt tgagggttta ttaaaattga ttagatctga
     1741 tccagcagct tgcgcttcgt caacggttat tgttctacga atataagttt tgaaaatttt
     1801 tgcaccaatg actctagaaa tttcggttgc tgcagtcgtc atttctttag aaatgttata
     1861 acgcttatta tacttagtaa ataggactcc tcgtacttca agcttgtcgt tcgtatattc
     1921 ttttaccgtt tcaatagttt cacttaactt tattagtcct tttaatgaga aatacgcagc
     1981 ttcaactgga atgataattt catcagaaac agtaaatgcg ttgactgtaa gcattcctaa
     2041 acttggtgga caatcaatta taatataatc atagttgtcc ttgattggtt gaagtactct
     2101 tttaagcctg tgttcacttc ctacttgagt aaattccctc tctgcagaag aaagggctat
     2161 gtcggctggc aaaacatcaa ttcctttata gtccagaata gcatctgtaa tgtcagcctc
     2221 cccttttaat acttcataaa ttgtaggttg gtttgttcca tctatatcga aacctacatt
     2281 atctgtagca tttccctgag gatcaatgtc cactaaaagt acatttttac cgtaaacaaa
     2341 tttcaaacat gctgctaaat taacgctagt cgtagtttta gaaactccac ctttttgatt
     2401 ggctactgac aatattcttg ccattaaaat atcccccatt catcttatat tataatataa
     2461 ttatatcata atattgtgat attgcaatat cgtatatgca ttatattgta atattgtgtg
     2521 attattttgt tttaaagata tatatcggtt cggatataaa gattattcat atgttattcg
     2581 aaagtaaaat aatcactttt tattacttta aaatttttta ttaatcataa gaaatatgtt
     2641 ttgcgaggag agtgtaaaat attttgtgta aatgaaaaaa tccatacaaa aaaggaagtc
     2701 gcttctgtag aataaagtta acgacaacca attcacagaa aagaggactt ccctatgaat
     2761 gattttacta cagaaattgt gcaaactcta gtcactaaag gcggtttaaa tgaattattc
     2821 cgttcgcact tagaaaaagc gataaacaca ctcctacgga ctgaattaac ggctttttta
     2881 gattacgaaa aatatgatcg cactggtttt aattcaggta attcgagaaa cggttcttac
     2941 tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg aaatacctag agatcgtaat
     3001 ggtgagttta aacaacaaac tttaccagcc tacaaaagaa caaacgatac attggaaacc
     3061 actattatcc atttattcga aaaaggtgtt acgatgtctg aaattgctga tttgatcgaa
     3121 aaaatgtacg gtcatcacta tactccacaa accatgtcca acatgactaa agttctgact
     3181 gaagaagtaa atgcctttaa atccagagcc ttaaatgata agtatgtcgc tatttttatg
     3241 gacgctactt acattccact aaaacgtcaa accgtatcca aagaagcgat ttatattgcc
     3301 attggtatac gagaagacgg cactaaagaa gtactgagtt atgcgattgc tccaactgaa
     3361 tcaacatacg tttggaatga gctgctacag gatattaact ccagaggagt tcaagaagtc
     3421 ttgcttttta ttacggacgg cttaaaaggc atgaaagata ctatccatca aatttatcct
     3481 aaagcaaaat atcagcattg
//
LOCUS       DAGCXG010000147         3485 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGCXG010000147 DAGCXG010000000
VERSION     DAGCXG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.153"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..681
                     /locus_tag="MB022_002605"
     CDS             <1..681
                     /locus_tag="MB022_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBT4467567.1"
                     /translation="KRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRAGAV
                     GIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIALDC
                     TLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEE
                     SKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIA
                     SRFVKALS"
     gene            695..2197
                     /locus_tag="MB022_002606"
     CDS             695..2197
                     /locus_tag="MB022_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4467568.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSSKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIISSVFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVDIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2210..2686
                     /locus_tag="MB022_002607"
     CDS             2210..2686
                     /locus_tag="MB022_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4467569.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTLETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2845..>3485)
                     /locus_tag="MB022_002608"
     CDS             complement(2845..>3485)
                     /locus_tag="MB022_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBT4467570.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1111 a          493 c          685 g         1196 t
ORIGIN      
        1 aaaaggtttt tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt
       61 gaaccatttt acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg
      121 gctggagcag ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt
      181 gtatcattac caatgattgg aattattaaa agagattatc caccagaatc tccattcatt
      241 actgccacta tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt
      301 gattgcactc ttagaaatcg gcatgatgga ctatctataa attcttttat tactaaaata
      361 aaagaaaaat atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa
      421 aatgcatata aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa
      481 agtaaaaaac aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt
      541 tgtgtaattg ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata
      601 ggagttgcag gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga
      661 tttgttaaag cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg
      721 acgcgctttc atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg
      781 aggagcgtta acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt
      841 gcatacaatt ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact
      901 aatttttgca attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt
      961 agctggtgga gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt
     1021 ttcatcaaaa gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac
     1081 tgttgcattt ttacacaatc gatatagaaa aattgaatta ccacaattct taggtttttt
     1141 tggaggatca agatttattc cgattgtttc tagtttagct gcaatcatta ttagttctgt
     1201 cttttactta atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc
     1261 tcaaatgggt agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt
     1321 tggactacac cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac
     1381 tgtagctgga tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc
     1441 aaaccacact ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat
     1501 gatgtttgga ttacctgcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg
     1561 taaaaagaat ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac
     1621 tgaaccagtt gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt
     1681 cttagatgga ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt
     1741 ttcaggtggt ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa
     1801 ttggttaaaa ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac
     1861 attctttatc aaaaaattta aggtagatat tcctggaatg gaagaagaat cagaagttca
     1921 ttctgaaaaa gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga
     1981 agctcttggt ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt
     2041 tgctgttacg aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc
     2101 tgtcttcgaa gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag
     2161 tcaagaaatc aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat
     2221 tttcaaaaag aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat
     2281 cacagaagta aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat
     2341 acctgatggt tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac
     2401 taaacatgct attaatataa aaagcaactc aggcattgat tatttgattc atattggaat
     2461 tgatacagtc gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt
     2521 tactcttgaa acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga
     2581 tacttctgtt atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa
     2641 tggtcgaatt gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa
     2701 aaagctataa aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac
     2761 tgttttcttt ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga
     2821 tccgtaattt aaagttatga tttgtcaaat taagctagaa taaaatgctt cttgtacata
     2881 gctcaaaaat atttcgattg gtgttctgta attcaatgat tttcttggaa tatgattacg
     2941 ttgattgctg acactggaaa taaatgtctg attcactttt ctaaaatcca ttgatttcgg
     3001 cagtccatta cgacgcagaa tcccgttaga attctcgttt aatgggcgtt gagaaggtgt
     3061 tccagggtcc gcaaaatata tatcaatatc atgttggtta ctaatggctt tccagttaga
     3121 aaattctttt ccacagtcaa acgtaataga tttaaagaag tttttaggga agcgagaaaa
     3181 ccattgatta agggcagttt caatatctaa tgccttacgg ccgttgggtt taatcgtgat
     3241 aatgacttta gataatcttt caactaaagt aatgacggca cttttatgat gaatgccaac
     3301 gatcgtatca ccttcaaggt gaccaaactc agaattgaaa tcaggataat tatcaggtcg
     3361 atcatggatt gagcgctgat actgttgttt tccccgtttt tcctgatggc cattgggttt
     3421 tcttttacct ttcatcggta gtgtgtcaat atcaaatatt cctttagaaa ataaacgata
     3481 aagtg
//
LOCUS       DAGCXG010000148         3421 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.154, whole genome shotgun sequence.
ACCESSION   DAGCXG010000148 DAGCXG010000000
VERSION     DAGCXG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.154"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..2232)
                     /locus_tag="MB022_002609"
     CDS             complement(<1..2232)
                     /locus_tag="MB022_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467571.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="MB022_002610"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="MB022_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          949 a          758 c          547 g         1167 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtattgcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagttttact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 a
//
LOCUS       DAGCXG010000149         3220 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGCXG010000149 DAGCXG010000000
VERSION     DAGCXG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.155"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            14..175
                     /locus_tag="MB022_002611"
     CDS             14..175
                     /locus_tag="MB022_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBT4467572.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            172..477
                     /locus_tag="MB022_002612"
     CDS             172..477
                     /locus_tag="MB022_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467573.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            477..833
                     /locus_tag="MB022_002613"
     CDS             477..833
                     /locus_tag="MB022_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBT4467574.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            820..1038
                     /locus_tag="MB022_002614"
     CDS             820..1038
                     /locus_tag="MB022_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467575.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            1059..1148
                     /locus_tag="MB022_002615"
                     /pseudo
     CDS             1059..1148
                     /locus_tag="MB022_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1223..1690
                     /locus_tag="MB022_002616"
     CDS             1223..1690
                     /locus_tag="MB022_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBT4467576.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            1965..2201
                     /locus_tag="MB022_002617"
     CDS             1965..2201
                     /locus_tag="MB022_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467577.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            2225..2566
                     /locus_tag="MB022_002618"
     CDS             2225..2566
                     /locus_tag="MB022_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467578.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(2559..2750)
                     /locus_tag="MB022_002619"
     CDS             complement(2559..2750)
                     /locus_tag="MB022_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBT4467579.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            2960..>3220
                     /locus_tag="MB022_002620"
     CDS             2960..>3220
                     /locus_tag="MB022_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467580.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGII"
BASE COUNT         1175 a          455 c          683 g          907 t
ORIGIN      
        1 gaggtggtcc atcatgacaa cagaagaagt gattcaaatg cgtattcgaa acattcagcg
       61 tgaaattgac gatctagaac gaacaaaggc agtgatggtc aatgaaacgg cgagaaaggc
      121 aatcgatttg cacatagaga acttaagaag ggaaattcgt agattggagg aatgagcgtg
      181 gataagaaag cgacaatgaa acgaattgct gaattaacca agtcagaatc ttggcaggaa
      241 gacaaagaaa tagttgcaga agtccaaaag ctcggtaaat caatgtggac tgaaaagcct
      301 aaacggaaaa cgccgagaaa aattgcaatc tggcatggtg atcgaattct agtaacaggt
      361 acagctgaac agttatctga aattactgga ttaagcaaaa acattatctg ggatagagct
      421 aggagcttat ggattgattc aaaaggacga cagtttaggt atgtggagga gagataatgg
      481 atctcattac acaatacagt gacatcatcc tcaagaaaat catgatgaag attcagaaag
      541 acaaaaaatc aaaagaacga gctgaattag ttaagttaga aatggctgaa acaggagcag
      601 gagtgcgaag tagcaggcat tggaaagcag cagcaaacat tgaattttat tacaacgaaa
      661 ttcaaaaagg gttcgatcag atgcgtgagc tggatcggca aacaaattgg agcaagaaac
      721 ttcatcaaga tcgtttcaaa tttgtagaga agtataaaga aatattagaa gagtatttga
      781 ggaggacagc aaatgataaa aaaactcgtt caattcagca tggatttata tgatatcgaa
      841 tcaggagcaa cactatctgt ggaatcggac catctaatca taaattttgg tggaaagcgc
      901 cagattattt tgtgggtagt tgatgatgta ctgtttccag aaattgttca tgatttcgaa
      961 gaatcaaaag cggttgagtt tgaaatagtg aaaaaagtaa tggaattgat tgaaaaatac
     1021 gaggaggaca gcgaatgata ccgaagttta gagcgtggga tagagaaaaa gacagaatga
     1081 aggcagtagc tttggttgaa tttgctgagg cgtataaatc aggagaactc atttcacgta
     1141 caaaataaaa aaagtcggat tcctccgact atgaataata tttccgacat aagtattata
     1201 tcataattgg gggaatcaga ggatggtact tttcgatgta aagaaatatg agacaccaag
     1261 cgcgaaagat gttgatatgg aacgcacaaa acataatgtc gctgtgtttc tttcagcata
     1321 tctatcagct agatgtagag tagggcaacc tcgagaacca aaagtgacag catcttattc
     1381 cttggttcca ccttctacag ctgatcatat atttgaagca gaaagaatgc tgatcgataa
     1441 agaagaagca caggaagaat ttgagtatct gcataaattg tttgttcgag gctattcagc
     1501 gatacagcat ccgcataagc ctgatgtgac tgaaaggcgc aagaagatat tctatgatcg
     1561 ttatatcaat ggtctgccca tttatgtaac tgctcaaagg aataatacta gcgaagaatc
     1621 ggttaaagta gaatcaaaca gaattatcat ccaatttgct tcatcgttag aactggttgc
     1681 tttcaagtag ccagttttta cactttttat acccttttat taccaatttg gtttccattt
     1741 tatacctttt ttataccaat cacttaccta ttcaacgttg tattatgata gtgtcgaaag
     1801 attaggaaac aggatcgaca aaataaaatg taagggagga aatctccctc atcgtttaat
     1861 taagcttcga taggcagcag cggaaatatt aagaataagg atgtgaattt caactccttc
     1921 taaattgttc ttattatcta tcatccgttg ctgtctatta atttatgtat tggagggtat
     1981 ataaaatgaa gatcgcttcc gaaaaaattg atcaactaac aattttagta aaccaaagaa
     2041 tcgaagatat tatcaaagaa ttcgaaggtg aaaagattaa gaacataaag cttactttaa
     2101 tggatcataa gttcgaatta aattcgacaa aaaggaagat cgttgagctt atagaagaag
     2161 aaatagctct ccagatcaat gagcaacaat tagctaatta atttatgtat tggaggggaa
     2221 atgaatggaa gtcgaacaaa taaagtatca aggtatggat cgggaacggt tgattgatat
     2281 tattgagcaa caacgtttag aattgatcca aaaagaagct gtatgtaaag agtacaaaaa
     2341 acatttggaa caagtaattg agtatcattc agtagaaaag tacagatcag tggtgcaaaa
     2401 aaatagagca acgagtcctg attctcgtca agatgtatcg attagtatca agacgccagt
     2461 atttaattct gttaaatcag aaaagctaga aagtatctca actgctttgg tagaaaaagc
     2521 agctgaaatg aactccctag caaatgaagt tagtcgtctt atttagtcat atcgataact
     2581 tgagagtctt tggtgattcc gttttttata gaattaatgg tttgttcagc ggaatattta
     2641 gtcaagtatg tttcgctagt tgctactact tcattgttat tggacttaat aacgaagtaa
     2701 tattggccat tagttgcttc tcttataaca aagtacaaat ttttcaccac ctataattta
     2761 tttcagcgga ccactcgctg ataattaaaa ttataagcta tgtataaaat ttcacaatat
     2821 caatttgtcg ctgtggcgga aagggtagac gcaataaagg atgagtggga aaagtcaatg
     2881 agtaattatc tgaaatttac aagtcccctg tttagttatc taagattatt tgataatatt
     2941 atttctgagg tgatagaaaa tggtgggcgt agttaaattt cttgggttta catttgatgg
     3001 atatggtacg ttaggcgaat gggtaggtat tattttgata gtgattacaa ttcaacaaac
     3061 atcaaagtac tactggtatt ctaatagttc taaaattgta tcaagaataa ctgaaggaat
     3121 aaccaaaaaa gatattactg tcgtcggtaa atatgggttt actataactt tattaaatga
     3181 agggaaatct gaaaccgcaa taaagttttg cgggattata
//
LOCUS       DAGCXG010000150         3180 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGCXG010000150 DAGCXG010000000
VERSION     DAGCXG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.156"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            62..817
                     /gene="fabI"
                     /locus_tag="MB022_002621"
     CDS             62..817
                     /gene="fabI"
                     /locus_tag="MB022_002621"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBT4467581.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(927..1940)
                     /locus_tag="MB022_002622"
     CDS             complement(927..1940)
                     /locus_tag="MB022_002622"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBT4467582.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2105..3025
                     /locus_tag="MB022_002623"
     CDS             2105..3025
                     /locus_tag="MB022_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBT4467583.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          934 a          643 c          605 g          998 t
ORIGIN      
        1 gctattattc tctatttttt tacgactatc aaatttttat cagaaacaaa ggagagtcat
       61 tatgtcattt ctagaaggaa aaaaaatcgt cattatgggc gtcgctaaca aaaaaagcat
      121 tgcttggggt tgtgctgaag caatgttgaa acaaggggca gaaattatct atacttacca
      181 aaatgaacga atgaaaaaat cattagtcaa attggtcggc gaagaagcat tcactgtaga
      241 atgtgatgtt tctagcgacg aaagcatcga aaaagcaatg aatgccatcg gtgaatatgc
      301 tggggagatc cacggccttg tccatgcagt tgcttacgcc aacaaagaag aattgtccgg
      361 aaatgtgtct gatatttcca gagacggcta tcaattagct caagatatca gcagctactc
      421 tttaattgct gtatcaaaat acgcacaacc gtttcttgca ccaaacagcg ggatcgttag
      481 catgacttat ctaggttcag aacgcgcaat cccgaattat aacatgatgg gtattgcgaa
      541 agcttctctt gaagctgcta tccgttattt ggcagctgag ttctcgccta aaggaattcg
      601 ggtcaatgga atctcagctg gggcaatcaa aacattagct gtcacaggtg ttaaagacta
      661 tcaaaaactg atccagttat ctgaatctcg tacgcctgat cagcaaggtg tgacgatcga
      721 agaagtcggt aatgcttgtg ctttcttaat cagcccactt tccagcggaa tcatcggtga
      781 tattatctac gttgacaaag gtgtccatct ttcataaact tttgttgcat tctttaaagt
      841 aaaacatata aaaatcagga aaaaactttt taaagtctct tcctgatttt ttctttttac
      901 gtttttttca actcattact ttgaaattat tccacgactt caatccagcc ttctggtgct
      961 tctatgtctc caaattgaat acctgtcaat tcatcataca accgttttgt tactggtcct
     1021 acttccgttt cactataaaa tacatgaaga ttgttattgt gataaatccc accgattgga
     1081 gaaataacag ccgctgtacc acatgcacca gcttctgcaa attgatctag atcatcaatg
     1141 aaaatatctc cttcaattgc ttgcagtcct agtcgttctt cagctaactg caacaaggaa
     1201 tatttcgtga tacttggtaa gatagagggc gattgcggag tgatgaattt tccgtctttt
     1261 gtaattccaa agaaatttgc tgcacctact tcttcgattt tcgtatgtgt ggctggatca
     1321 agataaacac aatctgagta atccagttct tttgcttctg ttcctggaag taaactcgct
     1381 gcgtaatttc cgccgacttt tgctgcacca gtccccttag gagcagcacg atcgtaatca
     1441 gatacaataa aattcgttgg ttttagtcca cctttgaaat acgctcctac tggcgtacag
     1501 aagacgacaa ataaatattc tggagctgga tggacaccaa tgttttcacc gaccccaatc
     1561 agtaaagggc gcaagtataa tgtagcgcct gttccatagg gcggcacaaa atcttcattt
     1621 gcttttacca cttgcttgac agcatcaacg aacatctttt cagggaccat tggcatcagc
     1681 aatcgtctag cactttgatt catccgtcga ctattttgtt caggtcgaaa gagattgacc
     1741 ttgccgtctt ttcgacgata agcttttaga ccttcaaaac attgctgacc ataatgaagc
     1801 gctggagagc cttcatgaat cgtcaggtaa ttatcttctg tcaattctcc ttcttcccat
     1861 tgtccgtctt tccatttggc aataaaacgc caaggagttt tgatataaga aaaacctaga
     1921 ttgttccaat cgatttgcat atacttacca ccttcgttat tttctccaca ttttatcatt
     1981 atccatatta tttgtcattt tattttcagc ttttttcctt cattatagtt atttttgcta
     2041 tttccttttt tgaaaacttc ttggtacaat acagaaagaa tcaaatttta gaaaagagga
     2101 ttttatgttt tcattattag caactgccgg tacgacagac tcttctggcg agacattgac
     2161 agatcaagct gtcagcaaag taaatgcgtt tcagcggttt tgggacggga ttgattggga
     2221 cgccattttg gctgccttta tccaaaaaag tttatatctg atttttttga tcgtcctttt
     2281 ttggattctt caccgtgtgg gaaaatatct gattgataag tcttttaatc agtattctaa
     2341 aaaagccatt ctaaacgaga gccgcttaaa gactttacat agtcttttaa atactatttt
     2401 tcattatacc cttggtttct tttttatcta tgcaatttta tctgctatcg gtgtgccgat
     2461 cggatcactt ttggctggtg ccggaattgc aggtgtggca atcggtcttg gtgcccaagg
     2521 gttcatgagt gatgttatta ccggattttt tattatcatg gaacaacaga tggatgttgg
     2581 ggactacatc aagctagcaa acttaacgat tgaaggaacc gtcgtatctg ttggtatccg
     2641 tacccttcag ctgaaagcaa cggatggtac tgtccatttc atcccgaatc gaaacatcac
     2701 cacgatcagc aatctctctc gtgcaaatat gcaagtactt ttagatattc ggattgttcc
     2761 tgaagaagga tatgacaaaa tctatgagat cattgatcga gtaaatcgta cattagctga
     2821 aaaataccaa gaagagctcc aaactgaacc ttctatcttt ggattagtgg atcttggaaa
     2881 cagcaatttt gcgattcgta cggtctgcta cgcattgaac ggaaaacaat atgccttaaa
     2941 agaagagttc ttgagctcct atgtcaaaga attgacagct tctggtttca cgattccgaa
     3001 cagtccaatt gctgtcggaa aataatttgg acaaacgatg caacgaatct cactaaaaag
     3061 agcgtgggac aaaagtaaag aacacttttg tcccacgctc taaacacgta taaacggcgg
     3121 aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
//
LOCUS       DAGCXG010000151         3093 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.157, whole genome shotgun sequence.
ACCESSION   DAGCXG010000151 DAGCXG010000000
VERSION     DAGCXG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.157"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(953..1765)
                     /locus_tag="MB022_002624"
     CDS             complement(953..1765)
                     /locus_tag="MB022_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467584.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1928..2035
                     /locus_tag="MB022_002625"
     CDS             1928..2035
                     /locus_tag="MB022_002625"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4467585.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            2093..>3093
                     /locus_tag="MB022_002626"
     CDS             2093..>3093
                     /locus_tag="MB022_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4467586.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEY"
BASE COUNT          991 a          488 c          614 g         1000 t
ORIGIN      
        1 gaaaaaaaaa gaaagtttct atacatttta aatattttat tcctatttct attgctaaaa
       61 aagtagaaat tctatattta cctttaactc tcgcttctct tgcaaatgta gttagcattt
      121 taatcgataa taaactcagg tagttatatt accttgaaat gattgataat tttgtcactt
      181 ctattttttt tgcaataaat tttttatttt agtgaaaaat aagaaactat ttctttgaag
      241 gcgataagtt agaaaaatat tattgtagtc ttttctttat taggaatcat cactgttgta
      301 tcttattacc ttttctgttt ttcttattag agaaaaataa tttgagaggt agatagtttt
      361 tattggagga agtaattatg tatatttttg aaacaaaaat ttttcattgc aataattgca
      421 acaaagaaat ttcatcgggg gaagaggttt ggacaaaaat ggaaatttcc gccaaaagta
      481 actgctcctc aactgaaatc aattaaagaa ctagaatttg agaatgcact ggtaatttgt
      541 atgacatgta ctgatgaagt atttcgaaaa catttttaaa ggttgagatg tatatagatt
      601 taaccaatca tttatctctc tcggaaaagg aatattaact aaccagcggg ttatcaaaaa
      661 taggtaggaa aactgtactt aatggcttat ttcgcgctga ttatgagaat atcagtctgt
      721 ttgattaatt tttagggttc aaaatacaga gacaaactcc aggtcgggtt tcgccacaag
      781 cgaattaccc gatctggagt tttgtctatt gcaggaaggc gtagccggac tgcaggaata
      841 aagcccccgg ttctaacagg gggctaacaa agaacaggaa actgtgacaa ctacccattg
      901 gggagtgtcc tagcagtatt ttctttgtca cgtgcttatc aattttgtca cctcattttg
      961 atgatggata cgctgcattt aacaggcgtt ctttctcgac ttccatcacg aatggtagat
     1021 aaagatattg ctgttctagt agatattgat tgtcctctgt gtactgataa gaagcggttt
     1081 cttctgaata gaccaacggg gcaattcgca catcgctggt caattgatta attgagtata
     1141 gaaggatggc tttctcacct agttccttgg gtaaatgttt cccaaaatag gaataggatt
     1201 tttgatttgc taaatagacg agtatgcgat ccacactaaa gttggagaag gtaaagggca
     1261 tcatgtgcag aatatgattt tgattatcat gttgcaaata gagctgggct tccttaccaa
     1321 aattagttga accaagataa agctgctttt tttcatcgat acattggaaa gtaaactggt
     1381 cttgttcagt tggtggaatg agatttgtaa taaaaaattc aatcattcga tccatttctt
     1441 tttcactaac aaggtcagcg tgtttgattt cgtctactat gagaccttca atcttaggat
     1501 caagcttacc tgcctttttt tggagcattt ctaaaatgta ttgattctct tgatcaccat
     1561 acaaaagata ttcattggtg acatgaccta tctctgcgat ttggtgaagg gtttcggttt
     1621 ttggaaggtt gatcccacgt tcccagttat tgactgtact tcgcggtaaa tcaccaatta
     1681 gtttgccaaa tttctccata ctcaacttta attcttggcg aatctgccga attcgattgc
     1741 cgactgcttg cttgtctatc tccatgaaaa ctcctcctca ggataagttc ttaaatttag
     1801 tatacgtgtg tacttaaaaa aagtcaaaac atattgactt aaaataaata caggcgtaca
     1861 ataaaaatgt acttaaaata agttcaatag gttctttgac aagtaaatag cagaaggagg
     1921 tttgtcgatg aaccaagtta atcaacatga tctcggtgaa gccattcgag tttctcgaga
     1981 agaaaggggg tggacacaac gatatttagc agaaaaagtg gggatttcga gatagttatt
     2041 atcgaaagta gaaaaaggaa cgagacaatt aagtgaagaa aaattgaatc ttgtgttgga
     2101 tagtttgcaa gaagaggtcg ttccagttaa ccgtgttttg attgattatc taaccattca
     2161 ctttttctcg aatcaacatt taaagctaat tgaagagatt attgaaatgc ccattgaacg
     2221 ttttgaggaa ctggattatg cgccaaaggg ctatattggc caatacgtgt ggaatcaagt
     2281 aatcacgatt cggtattcca ttgacgatgc ggttaaagga actgtgatgg agttttcagg
     2341 tcaaggctgt aaacacttag caagatggct aaaaacacga aaaacaaatt ggcaacagtt
     2401 ttttcgtaag gtattagatt atcaggggaa ctttacacgg attgatttta ctttagatga
     2461 ttttgtgggg agtctcagca ttccagaact taagcataag gtaatactag gccatgtgtg
     2521 gaccactttt caagtgagtg aatctcatgg cggtacggat atcataaaca atgaatcaaa
     2581 tggtgagacc ttgtatttag gctcaaagaa gagtcagtgt cgtttttgct tttatcagaa
     2641 ggaccatgaa caacggaagc gacgaggaat tcctttagaa gaggcaaaag taaagaatcg
     2701 ctttgaacta cggtatcgaa aagagaaagc gcaaagcttg gcaaagatta tttcaaaaac
     2761 ccatgattta accaaactgt tctttgagtt acttaatgga gcaatttgtt tttatgaccg
     2821 tgatccaaat gatctaggtg caaaagttga tgccaaatgg gcagccttta ttagcaatta
     2881 tggcgcaatc accatttctt tagatacaat tcctcaaagt tttgagaaaa ctatgagttg
     2941 gttaattcat ggtgtttcac catcattagt ttttattgca caggtcgatc aaacgtttca
     3001 ttcttccttg ttgcctttga ttattgaaca aggagaaatg aatccaagac agatcaaact
     3061 tctccaaaat atgaaggcag acccggagta tta
//
LOCUS       DAGCXG010000152         3076 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGCXG010000152 DAGCXG010000000
VERSION     DAGCXG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.158"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            755..>3076
                     /gene="esp"
                     /locus_tag="MB022_002627"
     CDS             755..>3076
                     /gene="esp"
                     /locus_tag="MB022_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBT4467587.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
BASE COUNT         1124 a          453 c          597 g          902 t
ORIGIN      
        1 gaatttatgg attatcaaaa attggatcag ttaattgaaa gagaaagctg ggaagattat
       61 tacgtgggtc taactttttc agaaaagtta aacaactaca agttaataga ttataagtat
      121 ttgagtgaaa gcaattaatt attcatgtag agtactagac actttaaata tggacaattc
      181 aattataaag aaaaagaatc aataatttaa tgaggctgtg acaaatgtca ttttttgttt
      241 cgctaaaaac gagtgaaaaa atgttgataa ctccaatcat tcgtttaaga ttctaggaat
      301 cattcaggaa tgctgtgaaa ccgatacttt ataattataa agaaggtgat ttcaaattcc
      361 gaagaaggaa tttgaagtca cctttttact tatgtcacag tctcattcag taatgcggga
      421 taaggctcct tattccgcta tttgaatact ctgaagttta atctaagaaa gagtaatatt
      481 ttgaaaacat gtttcatcgc atagaaatga acttaatttc agaaagaata catacatatc
      541 tttttatatg ttttcagctg ctgaatggtt aaaaagcaca aatatccgaa aaaaacatga
      601 agaaatgtac tttttttgtt gtaatgtgca tgaaattctt taagtaaact agttaacata
      661 agtttagtaa gattcctatt ctgtgaataa tacgtatcag atttatcttt tattcttact
      721 aagaaaataa ctattaaggg agttgatttg aataatggtt agcaagaata ataagagagt
      781 atttctagaa aaaacaaaga aacgagtatt gaagtacagt atcaaaaaat taagtgttgg
      841 ggttgcatct gttttggttg gtgttggtct tgtcttggga acaactgaac tagttcaagc
      901 acaagatgaa atttctccaa gcaccccact tgaaacagcg ataagctccg tacaagtagg
      961 tgacaaagta gcttcgggga atacttttca ggagaatcct ggttatacga aaaattataa
     1021 cttttcggat ttacaattta gtcctcaaga actaactgga gacacactaa aaggcaatac
     1081 aattggattt gaagtttacg gaaaacataa tattgcagca tcaactaaaa actgggaaat
     1141 ccgtcttcaa ttagatgaac gattagctaa atatgtagaa aaaattcagg tcgatccaaa
     1201 gaaaggaatt ggatcttcta gacggacctt tgtaagaatt aatgattcgc tgggtagacc
     1261 tacaaatatt tggaaggtca attatattcg tgcaagtgat ggattatttg ctggggctga
     1321 aacaactgat acacaaactg ctcctaatgg ggtaattacg tttgaaaaga gcttagatga
     1381 aatttttaaa gagataggca tagacaatct caagaccgat cgtttaatgt atcgaatcta
     1441 cttggtaagt catcaagacg atgataaaat tgtaccaggt attgatagta cgggctactt
     1501 tttaacagat tcagatgatt tctataatag cttagatgta tctgaaaata atcctgacca
     1561 gtttaaacat ggatcagtaa gtgcaaaata tgaggaacct aatactcaaa caaaagatgg
     1621 ttcaggatcc acaggtgcta atggagctat aatcttagat cataagttaa cgaaaaatta
     1681 taatttctca tattcagctt ccgcaaaggg cacgccttgg tatgctaatt acaaaattga
     1741 tgaacgatta gttccatatg tagcgggtat acagatgcac atggttcagg ctgacaaagt
     1801 aacatatgat gtttcttttg aatcagggaa aaaagtggct gatttagcaa ttgaacggcg
     1861 caaagatcat gaaaattatg gtgtaggttc aattactgat aatgatctta ccaaacttat
     1921 tgattttgct aatgcaagtc cacgtccagt cgttattcga tatgttttac aattgactaa
     1981 gccattagat gaaatcttag aagatatgaa agcaacagca caagttgaag aaaataaacc
     2041 atttggtgaa gatttcatct ttgattcttg gttgtcggat acgaataaaa aattaatcca
     2101 gaacacttat ggaacaggtt attattattt gcaagatatt gatggtgatg gaaaccctga
     2161 cgataaagaa gagagcggag acacgaatcc atatatcggg aaacctgaat tagaagaagt
     2221 atatgatgtt gacacaacag ttaaggggaa agtattcatc cacgagttag cgggaacagg
     2281 tcacaaagcc caacttgttg ataaagaagg tactgtatta gcagaaaaaa ctatcgctcc
     2341 aaatgaaaaa gatggggctc caatttcaga tactgtagaa tttgaattta cgggtgtaga
     2401 ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa atcgtttctc caggttttga
     2461 taaaccagaa gaaggttcaa ccgttattaa ggaatcacca aaagcggttg ataaacaaac
     2521 cgtggtagtt ggatttaaac cagatgctaa agaatcaatt cggaataata aaaacttacc
     2581 tgaagatgca gagtattcat ggaaaacaga gcctgatact tctaacgtta ctgatagtac
     2641 gaaaggtatt gtaactgtta agatcggaaa tcgaactttc gacgtggatg tagagtttgc
     2701 tgtaaaagct tctcaagcta tggaaaatga tgcaacatac gtacctataa caacaactcc
     2761 agaaacgaca gttcaaagtg gaaaacctac atttgataaa ccagatgttc ctttagctaa
     2821 agatgccttt tcaattttag atgtctataa taaggacttc ggtaatgcaa gtgttgatgc
     2881 aaatactggt attgttacat tcactccagc taaaggtgta ggagaatcgg agccgattac
     2941 tggaacaatt cctattaaaa ttgtttacca agatggttct gtaggcacga ccgatttagc
     3001 agtaactgta agtaaagata tttatgaaaa tccaggagaa aacattcctg caggctacca
     3061 caaagtgacc ttcacc
//
LOCUS       DAGCXG010000153         3013 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGCXG010000153 DAGCXG010000000
VERSION     DAGCXG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.159"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            71..1735
                     /gene="alsS"
                     /locus_tag="MB022_002628"
     CDS             71..1735
                     /gene="alsS"
                     /locus_tag="MB022_002628"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBT4467588.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAARVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVGELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1748..2458
                     /gene="budA"
                     /locus_tag="MB022_002629"
     CDS             1748..2458
                     /gene="budA"
                     /locus_tag="MB022_002629"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBT4467589.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMYIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2588..>3013
                     /locus_tag="MB022_002630"
     CDS             2588..>3013
                     /locus_tag="MB022_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBT4467590.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLVNSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQA"
BASE COUNT         1004 a          504 c          684 g          821 t
ORIGIN      
        1 aactgtatca cttatttgtg attatttcta actaataatt aaaaataaag cataaaaagg
       61 acgatgttca atggctgaaa aaatgaaaca aggttcgaaa tctgtagtag aaagtttgat
      121 taatcatcat gttgattatg tattcggtat accaggtgca aaaatcgatg gtgtatttaa
      181 tgaattagag gatcaaggtc ctgaattaat cgtgactcgt cacgaacaaa atgctgcatt
      241 tatggcacaa gcaatcggac gtattacagg agagccagga gtagttatcg caacaagtgg
      301 tcctggtgct agtaacttag ctacaggttt ggtaacagcg acagcagaag gtgacccagt
      361 tttagcaatt gccggacaag taaaacgaag cgatctattg aagttgaccc atcaaagcat
      421 ggataatgca gcactgttcc agccaatcac aaaatacagt gcagaaatcc aagatccaga
      481 aacaatttca gaagtgattg ccaatgctta tcgtatggct aagagttcaa aaaaaggcgc
      541 gagctttatc agtatcccgc aagatgtagt ggatgcacct gtccaaggta atgtcatcaa
      601 gccattgagc gatcctaaat taggttctgc ttctgcagat gatatccgtt acctagctga
      661 acgcattaga gaagcaaaac taccggtact attagtaggt atgcgaggat caagtgaaaa
      721 agaaacgtta gccatcaggc aattagttga aaagacagca ttgcctgttg tagagacatt
      781 ccaagcagct agagtgatct cacgtgaatt agaagcgcat ttctttggtc gtgtgggttt
      841 atttagaaac caaccaggag atatgctttt gaaacgaagt gatctagtta ttgcaattgg
      901 ctatgatcca atcgaatatg aagcaagaaa ctggaatgcg gaaaaagatg cgcggatcat
      961 tgtgatcgat gaagcgccag ctgaaatcga tccttttatg cagcctgaaa gagaattgat
     1021 cggtgatatc tcagccactc ttgatttatt gacaggttca ttagagcctc aacaagtgtc
     1081 agaagatgca aaagaatatt tagcatctct tcaagcaaaa ttaacggagc gggacattgt
     1141 tcaatcaaaa ggagaagcag gtatcttgca tcctttagaa gttattaaca cgcttcagtc
     1201 aaaagtaaca gatgatatga ctgttaccgt agatgtaggt agtcattata tttggatggc
     1261 acgccacttt agaagttatg aacctcgtca tttgttattc agtaatggta tgcaaacgct
     1321 tggtgttgca ttgccatggg cgatttctgc tgcattggtg cgtccaaata cacaaatcgt
     1381 atcagtttcc ggcgatgggg gatttttatt ctctgcccag gatttagaaa cggcggtacg
     1441 taaaaaattg aatatcgttc atttgatttg gaatgatgga cactacaata tggtagagtt
     1501 ccaagaaaaa atgaaatatc agcgggcgtc aggtgtagac tttggtccag ttgattttgt
     1561 gaaatatgca gaagcttttg gcgctaaagg aatccgagct acaagtgtag gggaattaga
     1621 aaaagcttta gaagaaggat ttgcgacaga aggacccgta attattgata ttccgattga
     1681 ttaccgtgat aatgagaaat tgggagaaac aatcttacca gatcaattct attaaggaag
     1741 tgtcgacatg actgaaaaaa tcttatacca gcacgggaca ttaggagctt tgatggccgg
     1801 cttgatggat ggaacagaaa cgattgctca tatattagaa aaaggaactc tgggattggg
     1861 aacattgcat gggcttgatg gtgaagtcat ctttttagat ggcgtagctt atcaaggaag
     1921 atcagatggc tcagttgccc aattagatgg aagtgaactt accccctatg cagcaattac
     1981 agattttacg ccagatacta gtttttctgt ctcaaaaact gcagatggag aacaattgaa
     2041 aaaagacata ttagtaagag aagccgggga aaacctattt ttggcagtga agataactgg
     2101 attattcaaa aatatgtaca tccgtatcat gccgaagcaa gaaaaacctt acagaagact
     2161 agcaaaaatt tcagagtctc aaccagaatt ccaacaaagc aatgtccaag gaacattagt
     2221 cgggttcttt acgcctgaat tattccaagg agttgctgca gcgggatttc acctgcattt
     2281 tatcgatgat acacatacat ttggcggaca tgtaatggat tttgaagtgg ctgaaggaaa
     2341 agttgagatt agccgtatct cgtcccttgt acagcatttt ccagttgatg atccgacttt
     2401 tgttcacgaa acgattgatt atgctgattt agcagcagaa atccaacaag cagaatagaa
     2461 aaagacagcg agatgcgttc aaggtgatta acctgacgta tctcgttttt ttgaggagga
     2521 aacctagcgt ttacaaaaaa actgagctat aatcttctcc atagatcaaa aaggagcggt
     2581 ttttatcatg tggacagaaa aacagaaaaa agatgcatac gaaattttat tattacagca
     2641 acaaggatta ttagaagcag aagataactg gttagcaaat ttagtaaatt catctgcatt
     2701 gttgaatgag actttgcctg agactgtctt tgcaggatat tatttattcg atggtaatga
     2761 attgatttta ggaccgttcc aagggcgagt ttcttgcaca cgtatcaaga tgggaaaagg
     2821 agtttgtgga gaatcagctg aaaaacaaga aacattgatc gttgatgatg tcaaaaccca
     2881 tgcaaattac atttcctgcg attcagccgc acaatcagaa atcgtagtgc caatggtaaa
     2941 agaaggtaat ttaatcggtg ttttagatat cgattgcgga gtaacgaaag ggtatgatga
     3001 aatcgatcaa gca
//
LOCUS       DAGCXG010000154         2985 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.160, whole genome shotgun sequence.
ACCESSION   DAGCXG010000154 DAGCXG010000000
VERSION     DAGCXG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.160"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(227..1678)
                     /locus_tag="MB022_002631"
     CDS             complement(227..1678)
                     /locus_tag="MB022_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBT4467591.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(1790..>2985)
                     /locus_tag="MB022_002632"
     CDS             complement(1790..>2985)
                     /locus_tag="MB022_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4467592.1"
                     /translation="REVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVK
                     KDGTRFSQEYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKV
                     GIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCL
                     SESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNE
                     YQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQF
                     TNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNT
                     KRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTG
                     LNIET"
BASE COUNT          886 a          529 c          427 g         1143 t
ORIGIN      
        1 cgatacgtac acgaaatcct caagatagat aaattgtttt catctagtaa aacctgctca
       61 aaatatgagc ataaagacgg caataataca ctcgatattc gataatggac ttgttttatt
      121 tgtcaaactc atcgtaatcg agatatcaat tccagtctca atatcctgat caaaggtcta
      181 agaatccaac cattttcttg ttactttgtc agattgatta ccttttttag tctccgaata
      241 ataaggtttt cggatcaact aacatgctct gagaaagact actatctttg caaatttcca
      301 aatgaaggtg tgggccagta ctattcccag tacttcctac atagccaatt atttgtcctt
      361 gcaaaacatt atctccagtt tttactaggt attgttgctg gtgagcatat aacgcggttg
      421 atccatcttc gtgttgtatc accacgtaat ttccccaaga gtagtgaaac tctgctttaa
      481 caaccttacc agctttaact gcatgaatcg gctcaccctg tgtagctgcc atatcaagtc
      541 ctcggtgaaa ttcatttcca cgatttccaa acaaactggt tattgaataa ttatgaatag
      601 gcacttggta tccagtctta ctcatttgtg gacctttaac ttctttatca tagttggtta
      661 aatcataggt tttaattaat tcatttagct tcaaatagta ttgtgtatct gttgcatacc
      721 gtcctgtaag gaatttagtt gcttctcgat atgttttact attagctttt aatgctcctg
      781 cataaaaatc tttatttcca ctaattccct tagttaataa tttggcataa tcttcaaaac
      841 tttcctcata gttttcatac actctaaatt ttgattgggt cgtataaagc gttccattac
      901 ccaaatcttc gtttgttacc ataatgacaa agtttccatt ataagttcct tttatcccaa
      961 ataaattgta attcggtgcc tgagctaact ggctttgacc actggcagat tctaagatcg
     1021 cttgtgcaat cataacagaa gcatataaat catatttctg accaattttt ctcgcagact
     1081 caccaatttt cataataaaa ttttctacag attcattttt gtcaaaatga atttcttcat
     1141 taccagatag tattggttgt gattggaaag attctgttga ttctatttgc tgtggtgtcg
     1201 aaaaattttt tgtttgtcct tccgtttttg gcgcttcctt ttttgaagac tctatatggt
     1261 ttggtcttgt cactttcgaa gaagttattt cacttgtact acttgaagtt gttgaagatt
     1321 cggcacttga aatcgtacta ctttcacttg acgaagttgt tgaagactcg gcatttgaag
     1381 tcgtactact ttcacttgac gaagttgttg aagactcggc atttgaagtc gtactacttt
     1441 cacttgatga agttgttgaa gactcagcat ttgaagtcgt actactttca cttgataaag
     1501 ttgtcaaaag ttcactactc tctgctgtta catcggaatt actcatttca gtcgtctcgg
     1561 ttctttcttt tgcaattttg tatgttgaat tttcgcttgc caacacatgg aaaggtgttg
     1621 ctataaagag ttgggacatt aatataactg ctgatgcaag ttttaaagat tttttcattt
     1681 gatttacctc tcttttttct ctttaatagt gttttctatt aaaaattatg ataaatattt
     1741 tcaatgaatt gagtaataca gtatatttct tgatttcatt tagtaacgat tatgtttcaa
     1801 tgtttaatcc tgttactgcc tttaaaataa aattataggg ttggctaaaa ctaggatgaa
     1861 ataaataatc taacattttc aatttgtcta atgtaatttg ttcttctata gcaagagaaa
     1921 acattgataa tagtccagat aaatcttctt gactacaaag ttgtacacct actattctct
     1981 ttgtattttt ttcaaagcta attcttaatt ttacacgact attttttctc aaaaaaacag
     2041 gtctaatgaa atcttctatt tctatataat ctatagactc acttaataat cgtgctgttt
     2101 ttaaattcaa acctgctgcg taaaaattgt aattaaatat agaaaagcca tttgtaaatt
     2161 gggaaccaga aggtgtaaaa tcttgattgt caatatgttg agttgcaata taagctgttc
     2221 ttagagcatt agaaatagaa aaatcaacac tacttctatt aatggacgta aaaaaagttg
     2281 tagcacaatc cccaatagca tatatatctt ttatatttgt ttgctggtat tcattagtta
     2341 gatatgctcc actaacatct ctcaagagtt cctttttgcc aattggacta ttagatagaa
     2401 atcctatagc ttgtacgacc atatccatag agtattgggt accacttgta gtgactattt
     2461 tttcaatctt ttttcttcct aaatattttt taggactttc tgatagacaa agatgtattc
     2521 cattttttga tagtatttct tcaactttat ctgaaaaact acggtcgtaa taagtcgaca
     2581 acactctatc tgcaacatcg aaaagatata cttctttccc tctttttctt atggcttcag
     2641 caatttcaac tccaatatat cctgctccaa taattcctac ttttttaata gtattatcat
     2701 ctaaagcttt aaaaatctct ttagcatttg gaaaattctt tatcgaaaaa atattagcta
     2761 aattattccc cggtacatct aacataattt gctttgaacc tgtagctagt ataagtttgt
     2821 catattcttg ggaaaatctt gtgccatctt tttttacaca aataatcttt ttattttgaa
     2881 tatcaatttt gtcaactgct gtctccaaaa acaacttttt tatcttagaa tttagttctc
     2941 ttctattttg ataaaaaaga tcttctaaat tgctaacttc tcttt
//
LOCUS       DAGCXG010000155         2945 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGCXG010000155 DAGCXG010000000
VERSION     DAGCXG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.161"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="MB022_002633"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="MB022_002633"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBT4467593.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="MB022_002634"
     CDS             complement(1061..1981)
                     /locus_tag="MB022_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4467594.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="MB022_002635"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="MB022_002635"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBT4467595.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          823 a          601 c          509 g         1012 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaact
//
LOCUS       DAGCXG010000156         2790 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.162, whole genome shotgun sequence.
ACCESSION   DAGCXG010000156 DAGCXG010000000
VERSION     DAGCXG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.162"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            144..494
                     /locus_tag="MB022_002636"
     CDS             144..494
                     /locus_tag="MB022_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467596.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="MB022_002637"
     CDS             908..1726
                     /locus_tag="MB022_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4467597.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="MB022_002638"
     CDS             complement(1799..2158)
                     /locus_tag="MB022_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4467598.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          942 a          395 c          495 g          958 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt
//
LOCUS       DAGCXG010000157         2781 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.163, whole genome shotgun sequence.
ACCESSION   DAGCXG010000157 DAGCXG010000000
VERSION     DAGCXG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.163"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(74..1168)
                     /locus_tag="MB022_002639"
     CDS             complement(74..1168)
                     /locus_tag="MB022_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4467599.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="MB022_002640"
     CDS             1357..2118
                     /locus_tag="MB022_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4467600.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="MB022_002641"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="MB022_002641"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4467601.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          884 a          550 c          468 g          879 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt t
//
LOCUS       DAGCXG010000158         2779 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGCXG010000158 DAGCXG010000000
VERSION     DAGCXG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.164"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..197
                     /locus_tag="MB022_002642"
     CDS             <1..197
                     /locus_tag="MB022_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467602.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="MB022_002643"
     CDS             314..811
                     /locus_tag="MB022_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4467603.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="MB022_002644"
     CDS             786..1292
                     /locus_tag="MB022_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4467604.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2779
                     /locus_tag="MB022_002645"
     CDS             1276..>2779
                     /locus_tag="MB022_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4467605.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPK"
BASE COUNT          839 a          514 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaag
//
LOCUS       DAGCXG010000159         2747 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.165, whole genome shotgun sequence.
ACCESSION   DAGCXG010000159 DAGCXG010000000
VERSION     DAGCXG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.165"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..489
                     /locus_tag="MB022_002646"
     CDS             <1..489
                     /locus_tag="MB022_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HBT4467606.1"
                     /translation="YNILPIYNQLNDENKVATYEFASNRLEEQKQENVLDFPKKEKIP
                     TVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSMEPVIRNGQFVFVKE
                     QPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYEDRIVSPEQIRIIGKV
                     VF"
     gene            576..1865
                     /locus_tag="MB022_002647"
     CDS             576..1865
                     /locus_tag="MB022_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBT4467607.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            1931..>2747
                     /locus_tag="MB022_002648"
     CDS             1931..>2747
                     /locus_tag="MB022_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467608.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQ"
BASE COUNT         1122 a          363 c          473 g          789 t
ORIGIN      
        1 tataatatcc ttcctatata taatcagctt aacgacgaaa ataaagttgc tacctatgaa
       61 tttgcaagta atcgattaga agaacaaaaa caagaaaatg tcctagattt tcctaaaaaa
      121 gaaaaaatcc caactgttca caattcggct tcggctgcca atccaacaga acttacttac
      181 ggtgatacgg ttgtcgaaga ggaagaattt gagagagttc caagtgctgc tgacttcgca
      241 gtacctatta taggcgattc aatggaacct gttatcagga atggacaatt cgtattcgtt
      301 aaagaacaac cggacgtaga agatggggaa attgctattg tggaattagg tggtgacgga
      361 gttacatgta aagagatata taaagattac gaaaatcaaa caatcatttt acgttctata
      421 aacgacttat acgaagatag aattgtatcg ccagaacaga ttagaattat cggaaaagtt
      481 gtattttaaa aaaattccca tgccggagcc gtcaacttcg gtaagggttt atatgatttt
      541 gacaattatt ttataaaaga agcgaggcgt tttgaatggg agtactattg tttttagttg
      601 gaatagtttt aataattatg ggtagtagca aattatctga agctaaaaaa ttaaaagata
      661 aaacttctgc cttagagagt aaggtaaagg aatattctca agaattaaat gctagggaaa
      721 cgtttatatc tgaaaaagag aaaaacataa gtaaattgaa taaaaacttg tcaacgctag
      781 aaaacaaaat tgatattctt aatgctgaaa gccagcggtt agacgatgaa attaatcaaa
      841 aatatgacac atctctggtc actatctata aatcagtaat tattccgaat atagatgaaa
      901 cttcttcatc cgaaataaaa aacaaacttt ctttattaaa gattaaagaa aaggatttta
      961 ttttagacgg tgcaattaat attccttctt atttgaataa tagagaagct aataatatta
     1021 aaaagaaaat gcttatacca ttagaatcag aaataactgg acttctcaat aaacttacta
     1081 tttctaacgt agattcaact agagaaaaaa ttataagaac ttttgataaa ataaacaagc
     1141 tttttaaaaa cgatgaagca gaattcaaaa aagaattact tgaattaaaa cttcaagaat
     1201 tagaactcaa ctattcatac atagttaaag ttaacgatga aaaagaacag caaaaagcaa
     1261 taaaagaaca aatgattgaa gaagaaaagg taagacgtga aatagaacgt gaaaagaaac
     1321 gcattgataa agaagaacga cagtttaact cagagataac taagttaatg gcatacatgc
     1381 aaaaagctag tgctgatgtt gaaaaagaac tctatgctga taaaattaaa gaactggaag
     1441 agaaattaag agaactagaa actgtcaaag aagatgtatt gcaaagagaa cttaatacta
     1501 gagctggcta tgtttatgtc atcagtaata tcggatcttt cggtgaagat gtctataaaa
     1561 taggaatgac tagacggtta gaacctatgg atagaattaa ggaacttagt agcgcttctg
     1621 taccttttga atttgatgtt catgcaatga tattttcaga agatgcacca acattagaaa
     1681 atcaactaca ttcgcatttt agaaaaaacg aagtcaataa agttaatcaa cgaaaagaat
     1741 tctttaaagt atctcttgat gaaattgaaa aagttgtttt agaaaattat aacggaactg
     1801 tatcttttac taaactagct caagctgaac aatacagaag atcattagaa ttaacagatg
     1861 tgtagtaaaa agattagcct tcgggctttt cttttacaac gataagaaca tacattcgaa
     1921 aggagataac atgaaacgag tagcattata tatgagagta tcaactgaac aacaagccaa
     1981 acatggagat agtcttagag aacaaaaaga aaccctctat gaatatatcg aacaacacaa
     2041 ggatttgaaa gtagttaatg aatatgtaga cggtggtatt tctggtcaaa aaataaatcg
     2101 tgatgaattt caaaaactat tacaagatgt aaaagaaaat aaaatagatc taattttatt
     2161 tacaaaatta gatagatggt ttagaaatct acgacattat ttaaacactc aagaaatttt
     2221 agaaaaacac aatgtttctt ggaatgctgt ttctcaacaa tattatgata caactacagc
     2281 gtatggcaga actttcattg cacaagtaat gagttttgct gaattggaag cccaaattga
     2341 ttcagaacgc atcaaagctg ttatggcaaa taaaattgca caaggtgaag tagtaagcgg
     2401 gaaaactccg ttaggctact ctatagaaaa taagaaatta gtaataaatg atgatgctcc
     2461 tatcgtgata gatatattca actactttct ttctagtggt agtttaagaa aaactgttta
     2521 ttatttaggc tctcagtatg gaatcgttag agattatcaa agtgttaaaa atatgttgac
     2581 gaacaaaaaa tacattggtg aattaagaaa taataaaaat tactgtcctc ctattattga
     2641 taaaaaactt ttttatgctg ttcaaaaagc cttacctaaa aatttaaaaa caaatgcaaa
     2701 acgtgattac atttttaaag gtttattaaa atgttctgac tgtcagg
//
LOCUS       DAGCXG010000160         2683 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.166, whole genome shotgun sequence.
ACCESSION   DAGCXG010000160 DAGCXG010000000
VERSION     DAGCXG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.166"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            17..412
                     /locus_tag="MB022_002649"
     CDS             17..412
                     /locus_tag="MB022_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467609.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            737..1354
                     /locus_tag="MB022_002650"
     CDS             737..1354
                     /locus_tag="MB022_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467610.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1395..1694
                     /locus_tag="MB022_002651"
     CDS             1395..1694
                     /locus_tag="MB022_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBT4467611.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1728..1895
                     /locus_tag="MB022_002652"
     CDS             1728..1895
                     /locus_tag="MB022_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4467612.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1940..2503)
                     /locus_tag="MB022_002653"
     CDS             complement(1940..2503)
                     /locus_tag="MB022_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467613.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2531..>2683)
                     /locus_tag="MB022_002654"
     CDS             complement(2531..>2683)
                     /locus_tag="MB022_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBT4467614.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
BASE COUNT          917 a          418 c          508 g          840 t
ORIGIN      
        1 tgaattgtaa aaggacatga tgattatgga agaaatagaa tggaataatc tgactcaaaa
       61 taataaaaat aattctggat gtggaattat ctttatgttt gttatagttt tgggaatagt
      121 agtactttta ataactgtca agatagtaga ttactttgcg aatactcctg aagaacaaat
      181 acagagaaaa cttcctgagt ttgaaagagt accaccttat gatccaagag ataattcatt
      241 tcaagtaaaa aaattaaaag agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc
      301 aagcgacttc aaagaaaaaa agaaagaata caattatatt gtaaaggcaa aagtatcaaa
      361 aagatttatt tttaacccta aattagaaat atatagtgtt gtaatagaat aatagtgtta
      421 aaaaaattgt aaaagttaaa gaagtcaaat tctgatgtga aaaataaaat ataccatgga
      481 agacataaaa gaaaaactgg ataactagtt tatttaatgg cagaccgtta aggcctgcca
      541 tttttatgta ttttagcgga ataaatataa tcatgcggga gtgtaaactt actgtttcaa
      601 aaagtatact ttttgacatg ccttgatttt ctttgtttat gtatggcata ataaccacat
      661 aatacaattt gaaacgtttc tgatttaatc tgattgtttt gaaacaattt tagtgaaaaa
      721 agggtagata aaaacgatga gaaaattttt ttatatccgg gtcagtagtt atgatcaatc
      781 gatcgaacga caactgattt ccgcgaagga attaggaatc cctgaagaat atatatttgt
      841 agaaaaagcc agcggcaaag attttaaacg ccctgaatat caattaatga aacggatgtt
      901 tcgaaaagga gatttactct atctacaatc attagataga ctaggcagaa ataaacaaat
      961 gattttagaa gaatggcgag aattaactaa ggaaaagcag attgatattt ctgtgttaga
     1021 catgcctctg ttagatactg ctagttacaa gcaactagat gggttagaga cgcttatctc
     1081 tgacttaatt ctgcaattac tcagctatat ggccgaagac gagcggaaac gaattaatag
     1141 ccggcaaaga gaaggcgtag aagctgcgaa acaaaagggt gtaaaattcg gcagaaagaa
     1201 aattgcttta cctcctgaat ttcctgcgat ctattctgac tggaaagcag gaaagataac
     1261 tgcagtctat gctatggatc aattgggcat gaaacgaaat accttttatc gacgggtgaa
     1321 agaatatgaa cataccttgc accacttttc ttgatagata taacagctat tagacgggaa
     1381 gggagagttt gaaaatggac ttaacgaatc aaagggtcgt ttttactgga acgctgcagc
     1441 aaatgacacg aacccaagcc atcggtttgg ttcactcttt aaacggccat tgtcaatcaa
     1501 gtgttacttc aaaaacaaat ttcctggttt gtggtcagta ttctaagtcg ttgtttgatg
     1561 attctctcta tacaaaaaag gaacaaaccg cgcgggactt gattgcgtta ggtcatgaga
     1621 ttaccatttt atcagaagtc gaattttatg cgttaattag tcaacagttg aaagaatggc
     1681 aacggtattt ataatcgtta gtaaaattta caaggaggaa aatttttatg aagcgaaaat
     1741 cttatgatcg ttggggcatt gaaataacta gttttaatcg aaatattgct tttcacgtgg
     1801 agtgtgactg tggggaatta gcaaaaagtg tttatcaaaa aaattacttt tactgtccta
     1861 aatgccatcg ccgatacggg gtacatggaa catagaaacg aaatagtgcg gtagaaaagc
     1921 gaatgaatgt agtatatttc tacaaatcat tcgctttctt tattttataa atagttggtc
     1981 gagagatccc gttttctttt gcaatctctg caactggaat gccttgcttt aacatgtcca
     2041 ccacggtttt aaaaactaag cgtttttgcg gatctgttga gttaatagaa tactcgattg
     2101 gtcgtccctt atatcggccg gctttttgag ccaataatac tccttgccgc tgacgttcac
     2161 gaattttctc acgctcgttt tgtgcaatat aacttagcag tgataaaaac atatcgaaca
     2221 tagcggtatc caggagttca tttccagtat taaaatctaa aaactgtgca tctaatattt
     2281 ttaacgctac ttttttctgt ttcataaaag caactgtatt ttttatatct tcataatctc
     2341 ttcctaaacg atctagtgat gtaacgatga cgcaatcacc ttcacggata taatttagta
     2401 atttttgaaa ctcaggacgc tcagtatctt taccactggc tttttcttgg aataatttat
     2461 caatgttaag tccttcggtt ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac
     2521 tccaaatatc ttaggctgtt attcccatta ataccttgat ttcagtagac accgaaaagc
     2581 cgaagagcgt tccatttctt cggttctttt tatatattcc tcgaatggtc tccatgccct
     2641 taatcgtgga agaggctgta cggagacttt gataaaattt att
//
LOCUS       DAGCXG010000161         2642 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGCXG010000161 DAGCXG010000000
VERSION     DAGCXG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.167"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            725..2551
                     /gene="ltrA"
                     /locus_tag="MB022_002655"
     CDS             725..2551
                     /gene="ltrA"
                     /locus_tag="MB022_002655"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4467615.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          949 a          461 c          585 g          647 t
ORIGIN      
        1 gaaaagagta ggcacagcac ttcattaaat caatatttaa tgtgtgttgg agaactaaac
       61 acacgaatag tcgaatgacg gggtaacgcc ctgaaaggct actttaatac tctgactggc
      121 agaaacttga gtaaacaagg ctctgtcaga agctcggtga agtcggcaga cagacaccgt
      181 aacagataat gctgacgaaa gattcccaga ggtgggcgtt gtgtcctata tgccggaagt
      241 ctataaaata tctatggtta gaatgtagat gagaggtcta ctgacgaatc cttgaatgta
      301 caggtctata tcggggctgg atagaaatgt ccagtgacac aaacagtgtc gctactgtgg
      361 gtgagtaaga ctccgcattt atgaaaatcc atataacgtt aaaggcgtta tcaaggtagc
      421 aggcttatag aggaaaccta aggatatatg tacagatagt gtgtttggaa cgttgaaagc
      481 tgacaacatg gagattttac tgtctgtgaa gtgttcacat cagaaagtta ttgtgagatt
      541 agcaatgata taaggtgtgt taatgtcagt gagagtggtg gcacagtacc tatgaaaccg
      601 tgataacaag cggtggaggg atagccacta gtcatatgaa gaatagaatt actaagaaaa
      661 atggtatggg ttcgagtaag actaagagat gtacttgctc ctgcaaacaa ggagggtaca
      721 gaccatgcta aagaaaaaca agctccgcta taacgagtat tatgatatgc aacatattta
      781 tgacgagtta tacgcacaga gcaagaacgg taataacttt tataagttac ttgaaattat
      841 tggttcggaa caaaatattt gtttagctta ccgtaatctg aaatcaaaca gtggcagtaa
      901 aacggctgga acggatggaa tgacgataga cgatataaaa caactcagca atgctgagat
      961 tgttgctacc gttcgggaaa gccttagcaa ctaccgcccc aaatcagtca gacgtgtttt
     1021 cattccaaaa gctgggagcg ataaaatgcg tccattgggg attccgtgta tctgggacag
     1081 gctggtacaa caatgtatcc tgcaagtcct agagccgata tgcgaaccaa agttccataa
     1141 tcattcctat ggattccgtg cgaatcgaag tgcccaccat gcagtcagca gagtaacgac
     1201 actgataaat ttatcaaaat atcattactg tgttgacgta gacattaaag gatttttcga
     1261 taatgtcaat catggaaaac tattaaaaca aatctggaca cttggtattc gggataaaag
     1321 attgatttgc attatcagca aaatgctgaa agccgaaatt gacggcgagg gtgtcccaga
     1381 aaaaggaacg ccacaaggcg gtctgttaag tccactgctc tccctcatag tccttaatga
     1441 attagactgg tgggtaagca gtcagtggga aacattccag ccgaagaacc gcagtaaaaa
     1501 tgggtggctt caatacgcaa agaaatatac caaactgaaa agcggtttta ttgtacgtta
     1561 cgctgatgat tttaaaatca tgtgttcaac ctatggagaa gcacaaagat tttaccacag
     1621 taccgtagat tttcttaata aaaggctgaa attagaaatc agtccagaga aatcaaaggt
     1681 agtaaatctg aagaaaaatt catcagattt tctggggttc aaaatcaaag tcattccaaa
     1741 aggaagaacg aaacacggtt atgttgccaa aacagatatg aaccaaaaag cattaaagaa
     1801 agcgaaaaca aatctaaagt taaaggtaaa agatatagcc agacatacga ctggcttcaa
     1861 tatcagcaga tataatctga cagttatcgg tatgcagaat tactactgca tagcaactaa
     1921 cgtatataac aacctgacag aagtaagtta cgctctctta ccgaccatac gaatccgttt
     1981 aaggaacatt gcaaaatcag ttccatttga aagtacaagt tcagaatttc agagcaggac
     2041 aaaaggaatc agaccaaaaa caaaaatagt gatgattgcg gataatccgc tattgccgat
     2101 tcaaggagta caacacaaaa atcctatgaa tttctcgcaa gatatttgta attttacaaa
     2161 gcaagggaga aacaaggtgc atgaggacgt tgtagttgta acaaaagaag aaatccgagc
     2221 gttgttggaa aatgaaaacc ctgccgatag cgtagagttt aacgacaatc gtatttcggc
     2281 atatatcgca caacaaggca actgctatgt aatgaatcgt cgaggaactc cgtccactct
     2341 gatatgtatt cacaaatcag acaaaggaga taaccttgac agatattcta atctggcatt
     2401 tgtggaaatt cccattgcaa aggcaatcct aacagaatca gcagacgaag caaaatcact
     2461 gttaaaaggt tatgttctta atagtcagca gaagaaaaaa ctgaacagaa tacggacgaa
     2521 ttacggatac cagacaataa ctgggaaata acttataaat ctatttggat atgatggaac
     2581 gccgtatgaa gcgaaagttt catgtacggt gtgaagcggg ggaaaacttg gagataacat
     2641 ca
//
LOCUS       DAGCXG010000162         2635 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.168, whole genome shotgun sequence.
ACCESSION   DAGCXG010000162 DAGCXG010000000
VERSION     DAGCXG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.168"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            7..1398
                     /locus_tag="MB022_002656"
     CDS             7..1398
                     /locus_tag="MB022_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4467616.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="MB022_002657"
     CDS             1414..2454
                     /locus_tag="MB022_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4467617.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..>2635
                     /locus_tag="MB022_002658"
     CDS             2473..>2635
                     /locus_tag="MB022_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBT4467618.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIER"
BASE COUNT          914 a          412 c          491 g          818 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagat
//
LOCUS       DAGCXG010000163         2568 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.169, whole genome shotgun sequence.
ACCESSION   DAGCXG010000163 DAGCXG010000000
VERSION     DAGCXG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.169"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..962)
                     /locus_tag="MB022_002659"
     CDS             complement(<1..962)
                     /locus_tag="MB022_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4467619.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1261..1896)
                     /locus_tag="MB022_002660"
     CDS             complement(1261..1896)
                     /locus_tag="MB022_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467620.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..>2568)
                     /locus_tag="MB022_002661"
     CDS             complement(2009..>2568)
                     /locus_tag="MB022_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBT4467621.1"
                     /translation="KTNKKTPMILLIIGIIFTTVGFSLSSSNEANDNNKQESSSSDTQ
                     TTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTFEG
                     TVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFTGE
                     ISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT          720 a          431 c          394 g         1023 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgctgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttt
//
LOCUS       DAGCXG010000164         2553 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.170, whole genome shotgun sequence.
ACCESSION   DAGCXG010000164 DAGCXG010000000
VERSION     DAGCXG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.170"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            61..717
                     /locus_tag="MB022_002662"
     CDS             61..717
                     /locus_tag="MB022_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBT4467622.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            899..1429
                     /locus_tag="MB022_002663"
     CDS             899..1429
                     /locus_tag="MB022_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4467623.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            1975..2217
                     /locus_tag="MB022_002664"
     CDS             1975..2217
                     /locus_tag="MB022_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBT4467624.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            2214..>2553
                     /locus_tag="MB022_002665"
     CDS             2214..>2553
                     /locus_tag="MB022_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBT4467625.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIY"
BASE COUNT          939 a          333 c          514 g          767 t
ORIGIN      
        1 tgtaagacaa attccagttt gtatggggcg ataaaatcag aatttgagag aggattgaat
       61 atgaataagg aatggtctga acttaataaa acaatgcagg cacaaataaa aaagaaagat
      121 acttataaga ggggtattga tactttactt actttacgaa gtcagttaat acaaacctta
      181 gtatctttca aagaagaatt atgcagagaa gattttaact caatcccctt cataaacgct
      241 gatggttatc atagtaagac gattgcttat tctatttggc atattttccg cattgaagat
      301 atagttgtgc atacagtgat aaatgaagat gaacaagtat tttttgcagg caattatcaa
      361 gagcgtatca attcacctat cattacaaca ggaaatgaac tcatgaaaca gcagattgca
      421 gacttttcaa aacagcttaa tctggaagaa ttatatttat atattttcga agtatgggaa
      481 agcactgaga aaatgcttga acggttatct tatgatgaat tgaaaagaaa aataccgaaa
      541 gaaagaaaag gatacttaga atcgttgaat gtagtaaacg acaatgaaaa agcaatttgg
      601 ctgattgatt attggtgtaa taaagatatt tgtggactaa ttcaaatgcc gttttcaaga
      661 cattggatta tgcacacaga agcctgtttg cgtataaaga acaagataca ttcataggca
      721 aaaatttcag tttgtagaac taaggaaatt ggaatttaag gaggaataaa gtatggttag
      781 tgattttata agagcagcat ttccatggat tttaatgggt ttatttgtag caatcagttg
      841 ttcccttatg agtaaaaaag agaaataaat tcctgtttgt atttataaaa gagaaaaaat
      901 gagaaaagta gttttattta ttgccatgag ccttgacgga tatattgcgg acggtaatgg
      961 tggagtagct tggctaaatg gtcatggaaa tgacaatgaa aacatcgaca cttatacaga
     1021 atttaccaaa gatatagata ctgtcttaat ggggtggaat acttctcatc aggttgttac
     1081 tgaactttct ccgcaagaat gggtatataa taaatttaca acttatgtat taacacataa
     1141 agagtgcaat tcttcccaac aaattcattt tacaagtgaa aatcctgttt tattattgga
     1201 acggctaaaa caagaagcag gaaaggatat ttggatttgt ggcggagcaa atcttgttca
     1261 gcagttggta agcaaaaata tgattgataa atattacatt tctgttattc ctactctgtt
     1321 aggaaatggg gttcgtcttt tgggcaacat agacagggaa ataaagttaa ggctttgtaa
     1381 aacacagact tataacggaa taaccgattt gatttatatg cgtagataac tagagagaaa
     1441 cagaaaagct gttaaaccga cggagcgatc cgaccacgtt ggcgggaaaa tccttaagat
     1501 ttttccgcca tgcttgcaag ggggcggttc gcttcggcgg ttcggcgagc ttgcgagccg
     1561 ttcaaagccc ccgttatcta acaagggggg cacaaaaaac aagaaacact atacactaac
     1621 aagcggaaaa cgctgtattt ataaggcaaa accccatttt tacagtagtg atataaattg
     1681 tacctatatc actatgaaaa ttgaatagta aatatgagaa agacagttga tttcaaaaaa
     1741 taagaaattg attgtcgttt tcttttgcag aaatttaagt gcaggcaatt atggtagttg
     1801 tcgtaattgc tgtcagctca gtgtttatca ctgatggtgt gaaagattta cggttggaga
     1861 tgttcaataa gattattggt gcttaattat ttttattgta gtgctgatat gagaatgtta
     1921 taattggggt ataaaacata caaataaatt tgctgaaaaa tgaggtatca aaatatggaa
     1981 aatgaattac tggaaaactt aggaagactt cacacaactg aacttggcgt tattagaatt
     2041 aaaaagaact tatctttgaa tgtagaaaat gtaattgaat ggtgcaaaga aaaaattagc
     2101 ctttctaatg cggaaatcac aagaaaagga aaaaactggt atataacgat tgataattgt
     2161 attataacgg taaatgcgta cagttatacc gttatcactg ctcacaaggt aaaatgataa
     2221 atttttctat aaaggaatta aacgcatttg cagttattgg gcaggaagta gaactgacta
     2281 attatcaaaa aaagaatatt cagattagta cacagttttg gagaaagttt aacagtagtt
     2341 tgaaaaaagc gtatctttca caatcaggaa attgggtaaa atatgctttt atggaaagaa
     2401 gaaatggaaa actttattat ttctgttcta ttccccaaag aaccattacc ccagagggct
     2461 ttctgtataa agaaataccg tcttataaat atttggttgt ggagcatatt ggtgctatgg
     2521 ataaaatata tgaaacttat ggcaagattt atg
//
LOCUS       DAGCXG010000165         2541 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGCXG010000165 DAGCXG010000000
VERSION     DAGCXG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.171"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..627
                     /locus_tag="MB022_002666"
     CDS             <1..627
                     /locus_tag="MB022_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467626.1"
                     /translation="LLNFFKKRNSGKVFNSNGNVYYNYFYFVFWVAATLLGYDKIQIA
                     GIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSIEKENFDDIDGYDSVNLLIID
                     TYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLILEIKKTMDEIQKSDFKKVFVA
                     STSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNGKYSKKYRIFI"
     gene            665..1054
                     /locus_tag="MB022_002667"
     CDS             665..1054
                     /locus_tag="MB022_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4467627.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1038..>2541
                     /locus_tag="MB022_002668"
     CDS             1038..>2541
                     /locus_tag="MB022_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4467628.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPK"
BASE COUNT          832 a          402 c          513 g          794 t
ORIGIN      
        1 ttattgaatt tttttaaaaa aagaaattca ggaaaggtgt ttaactctaa tggaaatgtg
       61 tactacaact atttttattt tgtattttgg gtagcagcaa ctttacttgg ttatgataaa
      121 attcagattg caggaattcc tatacacatg caatataagt tggttttgag tggaattttt
      181 tctgaggtgt tgccagatat atacgatgat cattacgaca gtgatggaac atgtaaagta
      241 agcattgaaa aagaaaattt cgatgacatc gatggatatg actctgtgaa cttactgatt
      301 attgatactt acgatataaa aatgagtgaa ttatctatgg aaaatcaaac atatcctacg
      361 ataattgtta gagggaattc aatagatgga gttcgtaaag ttaatagatc tttaatcttg
      421 gaaataaaaa aaacaatgga tgaaattcaa aaaagtgact ttaaaaaagt atttgttgct
      481 tcaacatcta atccaaaaaa tagtatcaat attattaatt catcgtttag atttttcggt
      541 cgatcacgta ggtttaaact ttatgttctt caaaaggatt atgcaagcaa tggaaagtat
      601 agtaagaaat atagaatttt tatttaagaa ggaatttttc cttcttttta ttttggagga
      661 aactatgaaa aaaataaaaa gctacaccag tatttgggca gttgaaaaag tcatttatgc
      721 gattaatgac tttcagctgc cttttcctgt gacctttaac cagatggctt ggtttgtttt
      781 atccttgatg tttgtcattg tatttgccca tgtgcctccg ctatccatga tagagggggc
      841 ttttttaaaa tacttgggca ttccgatagc ggtcacttgg ttcatgtcgc aaaagacctt
      901 tgacggtaag aaaccattgg gatttttacg atcctttatc agctatcact tacggtttaa
      961 ggtcactttt gcaggaaaga aagttaaaga acagaagaaa cgatgggacg aaccaatcac
     1021 cttggtaagg agtgtgaatt atgtacccga ttaagtatat tgaaaataat cttgtcttta
     1081 accaagaagg cgagtgtttt gcttactatg aattagtgcc ttacaattac tcctttttgt
     1141 cacctgaaca gaagtatcaa gtacatgata actttcgcca attaattgcg caaaatcgtg
     1201 aaggtaagat ccatgcctta caaattgcca cagaaagtag tattcgtgcc acacaagaac
     1261 gttcgaaaaa ggaaattacc ggtagactca aagaagtagc aaagcaacga attgatttac
     1321 agacagatgc attagtatcc atgattggcg atagtcagat tgattatcgc ttctttattg
     1381 gctttaaact gatttcgaca gatgaagagg tcaatctaaa gaatctcagg aaatctttct
     1441 tttctggact tcaagaattt gtctatggag tgaatcatca tttgatgggt gattttgttt
     1501 ccttatccaa tgaagaaatt cgtcgctata ccaagcttga aaaactaatg gaaagtaaat
     1561 tggctcgaag atttaaagtc aaacgagtaa cgccgagtga cttgacgtat ttaattgaac
     1621 atatctatgg tgaaaaaggc acaccgtttg aagaatatga attccagcta ccaaagaaaa
     1681 agctgaaatc agaaacgctg gtgaaacgtt acgacttatt acgtcccact cgttgtttga
     1741 ttgaagaaaa gcctcggtac ttacggatgg aacatgaaaa tcatgagtct tatgtagcgt
     1801 acttaacaat taataccatt gtaggggaaa tggagtttcc ttcctcggaa cttttctatt
     1861 accagcaaca acaatttacg tttccgattg atacttcaat gaatgtggaa attgtgacga
     1921 ataaaaaggc cctagctacc gttcgtaata agaaaaagga attaaaagat ttagacaacc
     1981 atgcctatca atctgataat gaaacgaact ccaatgtatt agatgctttg gattccgtgg
     2041 atgaattgga aaccaccttg gatcagtcga aagaatccat gtataaacta agctatgtgg
     2101 ttcgagtgag tgctaaatca gtggatgaat taaaacgccg ctgtgatgaa gtgctcgatt
     2161 tttatgacga taccaatgta aaactagtcc ggccctttgg cgatatgatg ggtctacaca
     2221 aagaattctt accctcaagt aagcgataca tgaatgacta tattcaatat gttacgtctg
     2281 actttttggc gggacttggt tttggggcta ctcaaatgct aggagaatta gaaggcattt
     2341 actttggcta taacgtagat acggggcgca atgtttacct gaaaccggca ttggcatcac
     2401 aaggggtcaa aggctcagta accaatgctt tggcatctgc tttccttggt tcgttaggtg
     2461 gggggaaatc ttttagcaac aatctcttag tctattatgc cgtccttttt ggtgggcaag
     2521 ctgtgattgt cgatccaaaa g
//
LOCUS       DAGCXG010000166         2524 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGCXG010000166 DAGCXG010000000
VERSION     DAGCXG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.172"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(198..1538)
                     /locus_tag="MB022_002669"
     CDS             complement(198..1538)
                     /locus_tag="MB022_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4467629.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(1673..2386)
                     /locus_tag="MB022_002670"
                     /pseudo
     CDS             complement(1673..2386)
                     /locus_tag="MB022_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
BASE COUNT          753 a          455 c          476 g          840 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttggct cttcgtcaat gagtgttggt gaagaaattc aatagaataa
      181 tcgagatact cacacggcta gacagctatc ttgtctagcc gtgttgttgt tctggcttta
      241 gtcagttggg tagtgttctt agttttcttg aaatattttc ctcgatataa aaagattcgt
      301 tctcggtagt tttgaaagtt tctaaagcca taggcaattc gttttagtac tttgatatgg
      361 ttgttcaaac attctatagg gccgttggaa tagggaactc gcaacgctcg tttgatttct
      421 gttcgatatt tcttgaaggt cgtgaacact ggttgatagt gctctggcaa ctccttgtag
      481 tcttctttta ggagcgcgac gaattcagga acatcttgtc ttctgatagc ggaaagaaaa
      541 acctggtaga cttcgtagcc tcgctttaag gagtcatcat aatataatag ccgatcgact
      601 atctccgctt ctgtcagatg atctcgaaat gacggacgcc agtagcgatg ctgaccattc
      661 aattcccacg cattcttttg aagtagtctc cagtatttct ttaagtgctt cgaccgacga
      721 tccccttttt taaaggtctt cattacttga attcggtggt tggtaaaggc ccgaccgatg
      781 tgttggctaa tatgaaagcg atcggcgacg attcgggcat tcggaaaaca ctctttcacc
      841 aatgtgcgat agggtgtata gaaatctgaa accactagac gtacttgttc acggactttt
      901 ctagggtagc gcaagaaata gtcgcgtaaa ggattgagtt gccgattttc cacaatatca
      961 atcagttgat gggtcttgcc gtccatcatg acaaagctca tggctcccga gacgtttttc
     1021 accgatttga attcatcgaa acacaccact tctggtaacg gctggttcaa agaaacggtt
     1081 ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg gagaaatgtg cttcatacgt
     1141 gcaatcaaag acatagaaat aggctcactt aatagctcaa gaatggcttg tttgactctg
     1201 cgggcaatgg agcagcgacg atcgctaact gaatcttctg ctaaaaaagt ctttccacag
     1261 tgtttacatt tgaagcgctg tttgttaatc cgcacaatgg tcttgtagtt actgacatca
     1321 ttcaataaga tggatacttt tttccagccc catttaatga tcgtttgctt attttgatag
     1381 tggcaatgga agcattcctc tggttgatag gtcaatgtgc catcaaaaac aaaacatgtt
     1441 tcgtcattaa tgcgctcttt gcgaaaggca tcttcatgaa aggtaagatt taggtctaga
     1501 atatctagta actctttggt aaactctgaa taggacatct tgaaacactc ctttgaatgg
     1561 ttttcgtcga ctttattcta aaggatttca aggtgtctgt ttgcgtttat tagaaaaaag
     1621 aaaaagtatt ggcaacagaa ggcgtcacca acactagata ttatagatcc atataaagtt
     1681 gtgatatttt tccagctaaa gatccaccat taagaaattc ttcaatggct gtttgtaaaa
     1741 ctaagtctgc agactttcct ataggtgaat gaatatagtt agttatagca ataatttttg
     1801 cgccattttt tttagctatt ttcaatgaat cataagtatc tttcgttttc ccagataaag
     1861 aaaaaatgat tactaaatca ttaactgtta ataaagaagc gacttgggct tgaaaatgag
     1921 ggtctagtac agcttttgct tgaactccta cgcgaagaaa catattttct aaatcaagac
     1981 tagtatttcc actagatccc actccaaata tatatatgtt ttttgattga gtgatcattt
     2041 taatggcttc attcaatttt tcttcgtcta aaagcctaat agtggaatag agagcttctg
     2101 ttaaactatt agcaatttca tcatagtact ttacactagt cggatgttct tttttttgac
     2161 taaagtcttc ctttgcaatt tcaattttta aatcagaaaa accagaaaaa ccaatttttt
     2221 gacaaaagcg aataatagta gcatcgccaa cttttgtgtt tttcttaata tcactcatcg
     2281 tactataaat aatagttttc ccagaattta aaataaaatc agcgactttt ttttcagact
     2341 tcgttaaaaa tggataattt tctttaatta aatagatata actcatacaa actcctctac
     2401 aatttcaact tttctccatt atactccaaa ataataaaaa aaactcctaa atactctatt
     2461 tttatcttta cttttatgaa agcggatgct ttattatttt tggagttatt atccaaaaat
     2521 aata
//
LOCUS       DAGCXG010000167         2522 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.173, whole genome shotgun sequence.
ACCESSION   DAGCXG010000167 DAGCXG010000000
VERSION     DAGCXG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.173"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            678..1904
                     /locus_tag="MB022_002671"
     CDS             678..1904
                     /locus_tag="MB022_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4467630.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(2012..2482)
                     /locus_tag="MB022_002672"
     CDS             complement(2012..2482)
                     /locus_tag="MB022_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBT4467631.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          931 a          380 c          492 g          719 t
ORIGIN      
        1 gctaagtaaa tgcatttgtt cacttgacat gtcctaacta attatcaagc aaggaacatt
       61 ttcttcgtta gtaatggatt ttaaagaaat ttcagttttt ggatgttgaa taaaagacca
      121 tttgtgaaac cactagtata catactcatt accatgtcaa ttctattact ttaaaagaaa
      181 atcgttttac tttgaagagg agcatatcta tccattaaaa gatgatggta atgcaatcac
      241 tagttcgagt cattttaaga gccatgcagt gagctggaaa taaaacgaat agaagatggg
      301 tttaacaaaa ggaccagtca taggaagaaa ttgtcgtgaa atcgaatgat aatctgaacg
      361 gtaggcaaag actgttaaaa agatgggaaa ggtatgtgcg atcattttac tgcacgatta
      421 tttggaaaat accttgttgt tcgctttttc tggaaaaaat acataaaaaa agataaaacg
      481 attgcgaact gcgtgtattc ggtctttcga tctttttcac ggtatggtat gatagttaag
      541 aattttgaga aattttaaaa aataataatc gttacacaaa aatgtttttt aaagttttct
      601 tatgattaaa aagtgaattt gctgattgaa cagttatatg agacaatatt ttagaaaatg
      661 tgaaacagag gtgtattatg aagcgatggc aaaaagttct attaaccttg ttaggggtat
      721 tcgttttgct gattgccgga ttatcagcat acggaatcag atttatggga gaagcgagcc
      781 aaacagtaaa caaaatttct aaaaactcta atcgaatatc ctccaaacgt tctgagagag
      841 taagcattga tgacaaagaa cctttttcgg ttttgctatt aggtttagat accggtggat
      901 taggaagaac ggaacaagga cgttctgata cgatgatggt agtaacagtt aatccgaaac
      961 aaaaaaaatc aacgatcatc agtttggacc gagatattta tacgaatatc gtaggttatg
     1021 gaacggtaga taaattaaat catgcctacg catttggtgg tgtcgaaatg gccatggatt
     1081 caattgagca actgctcgat attcctattg atcattatgt aacgatcaat ttggatggca
     1141 tggaagattt gatcaatgcg gtcggtggca tcaaagtgaa taataaaatt gactttactt
     1201 tggatggtgt ccatgtacca gtaggtgaac aaacattgaa tggagaaaaa ggtttagcct
     1261 attctcgaat gcgacacgaa gatccagaag gcgatatcgg tcgacaacgt cgtcagcggg
     1321 aagtagtgac gaaaatcgtc aataaagttt taagtttaga tggcgtaagc aattatagaa
     1381 aaattttaaa agcagttgag aacaatgtga cgacggactt agattgggat gacatgcttg
     1441 atgtagcgac gaactacacc ccggcgtttg aaacaatcaa acaagaccag ttgcaaggcg
     1501 aaggacaaat gatcaatgat atctactatc aaattttagg aaaaaatgat cttctttcga
     1561 tccaaaatga actgaagaaa caattaaata taaaaacaag cgatacatta cctaatttga
     1621 aaaatgagaa tgcttcaatg atgttctttg atgattcgga agatggcgat tcgacaaacg
     1681 gtgatggatc gaatagtgaa gggtacaccg attattcttc ctattcagaa aacacatatg
     1741 atcagtacca agatccgaat acctattatc agcaaacaga agatacaagt cagtactata
     1801 caccaagtca agatactcaa agttatgatc cttacgctgg acaggaacaa aatggttatg
     1861 gttatgacca gaataatgag tatggaacaa caagtgggta ctagaaaaaa atagtttggt
     1921 taataaagag tgccacctaa attagacaaa aaatctaact taggtggcac tttttctatg
     1981 tgttaaatta caggacgtct ctttttagcg cttagtttaa aaaataaatt gaataattca
     2041 atagagttgc gaatagtacc cataaataat aaggaatcat gagccacatt gccaggcgat
     2101 gatgcagcca caactgatac ataagcactg acaagaaaat aactaaaagt gtaatatcga
     2161 ttgctgcaat gaaaatattt cttaaattaa aaaatagagc agaccatata aaattaaaaa
     2221 tgaactgaat cacaaaaaga ccgacaagat tgtttcgctg ttttttgttg ttgtgattaa
     2281 gtaacaaata aagagaaata cccatcaata tatacagtaa ggtccacatc ggcccaaata
     2341 tccagctagg tggtgcaaat ggtggaagtt gcaaagaata gtaatatttt ccgggatttc
     2401 cgctaagcaa ccctgataaa aagcctaata tgacaattcc tataacacaa acccaaaaac
     2461 gaatatcttt tatcatcttc attttactaa ctcctttcta taatattagt ttaccatgaa
     2521 gg
//
LOCUS       DAGCXG010000168         2489 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGCXG010000168 DAGCXG010000000
VERSION     DAGCXG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.174"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..687
                     /locus_tag="MB022_002673"
     CDS             <1..687
                     /locus_tag="MB022_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4467632.1"
                     /translation="WKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENA
                     WTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAG
                     NIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPV
                     VNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDA
                     GAVGGTLYAT"
     gene            677..1417
                     /locus_tag="MB022_002674"
     CDS             677..1417
                     /locus_tag="MB022_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4467633.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1414..>2489
                     /locus_tag="MB022_002675"
     CDS             1414..>2489
                     /locus_tag="MB022_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4467634.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTI"
BASE COUNT          940 a          308 c          470 g          771 t
ORIGIN      
        1 tggaaaaata ttaaagatac ggcatacaat acgtggattt ctataaaaaa taccatgatt
       61 aatacatgga ataacattaa ggactctttc tggaatattg ttactggaat tgttaattct
      121 gctgaaaatg catggacaaa tctaaaaaat ggtgtttcaa atgctattaa tcgggtaaaa
      181 gaaatcttta attcgttaag ggaagtcaat ttatttgaaa ttggcaagaa catcattgat
      241 ggacttataa atggtgtaaa agaaaagtgg aatgctttga aagatactat caaaggtatt
      301 gcaggtaata tcaaaaattg gattaaagga gctttagata ttcattctcc atcaagatgg
      361 atgcgtgata tggttggtaa aaatattgtt caaggtatta tcgttggtat tgataaagag
      421 caaagtaagt tagatcaaac catgacaaat ttagtaaaaa caccgtctgt tcaacctgta
      481 gtaaatggaa taagtcacct accaattaca caaacaaagc aaaatatatc tgctagtgaa
      541 acaaaagaaa ttcatttaca tttaaatgtt tatggagatt tacctgattc aatggttcgg
      601 aagattacca caaaaattaa aacagaattg acaagacaaa tgaaacgaga tgctggtgca
      661 gtaggaggaa cattatatgc aacttaagag aggacaattt tttattaatc aacattattc
      721 ttctgaattt aatgtgtata ttcaaaacag gcctgcttct gtttcagcta gtcgagtaat
      781 tgaattgaga gagcgagaag gaaatgattc tatcattatt gataaaggct attacagaaa
      841 tgtgactaga aagattgagt gttactataa agcaccttca attgatgtgg tgcaggagtg
      901 ggaagatcga atcactgaat ggttagatat gtcgtcttat agtgatttta ttctttacta
      961 tgatgaacaa tatatttatc aagctgtagt aatagaagga cctgaattta aaggaacaag
     1021 aaaaacagga aatattgtcc cttttgaatt tacagtgagc attcgaccat ttaaggaaaa
     1081 ttacaatggt agatttacta ttcgacaaac agaaactttt gaaatttata acccagagaa
     1141 gtattcttca aaaccgctta ttaaattgag tggttctgga gatgcttctt tttatattaa
     1201 taaggacaaa tatgatttga aatcgttgga cagagaatta tttatagatt ctaaattaga
     1261 agaggcttac cgaaaattag atggtaattt agaacatcag gatcaagtca ctttattttt
     1321 agattttcca tttttatatc ctggaaaaaa tgaaattaaa tggacgaaaa atattcattc
     1381 atttgaaata atgcctaggt ggtggagaaa agtatgaaac caagaatata tagtcctact
     1441 gaaacagatt tctcaacgaa tggcttagga attttaaagg attgtacaag atgtgaaata
     1501 tatgaagtag ctaatggaaa atatgaatta gagttggatt atcctttagg aactagattt
     1561 gatgaatatt ttgaaaatga ctatcaaata aaagcaaagc caaacgatca agaagagtat
     1621 catatctttt ttattgatga taaagatata gatacttttt taaatacagt aactatttat
     1681 gctcagagtc gtacaaatcg acttggaaga cgggtagtca ctcttgcgga agtagactct
     1741 aaaactggtc aagaagcgat atcaattatt gaaaccaaaa tggataaaaa atctgacata
     1801 cgactttatt ctgatattac ggccgtttct agcacaatct ttgaagcgag aaatgtttta
     1861 aattgtattg ctggtgaaca aggatcattg cttcaatatt ggggtggaga aattaaacgt
     1921 gaaccattta agctttcttt gttaaagcga agaggtcgca ataatgttgg aacgattcgg
     1981 tatggaaaag atatgtctgg tttaaaggtc aaattagatt ggacaggtat aaaaacaaga
     2041 cttattccat atgctgatcc tcaaagtgag gtaggtacga ctaatcgaat ttatggttcg
     2101 ccagtagata gtgcctatat taataactat cctgatgtgt atacagagca tgttcagttt
     2161 acagaagaac aaggagtaaa agacattaag agcttaaata aaatagctaa aaattacttt
     2221 aaaacgatca atcctggctg tgataagcct aaagtttcta ttacggttga atttgataag
     2281 ttgactgata gtgaagaagc gaaagaattt gcgaagatta gaaattatgg tctgtttgat
     2341 acatttaaaa tatatcataa aaaatataat atttatcttg aatcaaaggt tagtggacta
     2401 caatacgatt ctttatctga aaaaactttg aaattagaag caggagacat tcaagttgct
     2461 ttttatcagc aacaagctgt aactattca
//
LOCUS       DAGCXG010000169         2441 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.175, whole genome shotgun sequence.
ACCESSION   DAGCXG010000169 DAGCXG010000000
VERSION     DAGCXG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.175"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..174
                     /locus_tag="MB022_002676"
     CDS             <1..174
                     /locus_tag="MB022_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467635.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            167..307
                     /locus_tag="MB022_002677"
     CDS             167..307
                     /locus_tag="MB022_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBT4467636.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            377..796
                     /locus_tag="MB022_002678"
     CDS             377..796
                     /locus_tag="MB022_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBT4467637.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            793..1803
                     /locus_tag="MB022_002679"
     CDS             793..1803
                     /locus_tag="MB022_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4467638.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            1918..>2441
                     /locus_tag="MB022_002680"
     CDS             1918..>2441
                     /locus_tag="MB022_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467639.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEI"
BASE COUNT          887 a          365 c          497 g          692 t
ORIGIN      
        1 agtggcacag cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta
       61 ctgacaaaac aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct
      121 caattgacga tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc
      181 gggctttgaa aatataaaac aattttatga ttggggatgt tatacggacc aagatttact
      241 tgactatgta aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga
      301 aatttaatta agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttctttgcat
      361 aaaggagaaa tagatattgg aaaaatattt aaatacactc tcagtaatga caggggtgat
      421 aggcgggaca attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt
      481 tcttgtgggt gtggacttct taactggact tgctaaggca tggaaattga aagaaataag
      541 tagtgagata ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt
      601 tgcggttgac gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat
      661 gttctatgtt gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt
      721 gccagataag ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg
      781 aggaggaacc aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg
      841 ccaataaacg cctttgctta ctcgatcaac aatgaattca atttgggcgc aaacgaaggt
      901 agttctcaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
      961 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
     1021 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
     1081 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
     1141 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
     1201 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
     1261 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
     1321 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
     1381 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
     1441 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
     1501 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
     1561 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
     1621 gtaaatcaag cttataatac ctctggaaac gttggctatc atgtatcttt taacatgcgc
     1681 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
     1741 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
     1801 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
     1861 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
     1921 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
     1981 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
     2041 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
     2101 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
     2161 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
     2221 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
     2281 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
     2341 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
     2401 attgaaaagg cgttatttct tagtaactat ggagaaatat t
//
LOCUS       DAGCXG010000170         2435 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGCXG010000170 DAGCXG010000000
VERSION     DAGCXG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.177"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            14..1384
                     /locus_tag="MB022_002681"
     CDS             14..1384
                     /locus_tag="MB022_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467640.1"
                     /translation="MSFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIA
                     LTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLS
                     KQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISD
                     SIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWF
                     LFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSI
                     AAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVG
                     ESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNV
                     DVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEIN
                     KKNENK"
     gene            1371..>2435
                     /locus_tag="MB022_002682"
     CDS             1371..>2435
                     /locus_tag="MB022_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467641.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILT"
BASE COUNT          804 a          370 c          418 g          843 t
ORIGIN      
        1 ttgtacgatt ctgattagtt tttattatgg agaaaagttt tttaaaaaga tagatgctgc
       61 ttttttattt agttgtttat atacttttgc aacttatcga acagttgata tctattatcg
      121 atccgcaatt gcagaagcaa tcgctcttac gattatccca gctgttttat attatgctta
      181 tggattgata ttcaatagag aaaaaaaagt catcgcttta tctgtcagta taagtctact
      241 tgtgtatact catgtcttaa ctacactatt gtgtagtgca ttgattgctt gtatgattat
      301 agttcgtttg tttttaagca agcaaataaa aaacgatata aaatttgtgg tcgtgaattt
      361 agtaaaggct gtttttttga cttgtgcatt aactagctat ttttggtatc ctatgttgca
      421 gcagatgaat tatcaagaaa tacacagtcc cttcaaaaca gatttgcagc tagaagcact
      481 ttctatttca gatagtatca ttcaagcgtt aaataacgat attacaacat acactatggg
      541 gttattagga ttggttgcgt tgatttttcc tttattcata ctgaaagaaa tggagaaaaa
      601 agaaaaaatc atctatgttg gcgtagtttc tacatgggta tgcgtaacta atttatttcc
      661 atggtttttg tttcaaaaaa caccgataag cttactccag tttccttggc gtatcttagg
      721 tatccaaatc tttttttctt caatgatcat aacgcttgtt tttatgaaaa agactatgaa
      781 taaaaaatat tcatggttaa tcattggcgc tactgtggtg tttctactcg tcacgtctat
      841 agcagcaaaa gaaaattacg caaaagttat ccaatcaaaa cacggtcata ttgttatcaa
      901 tgaagaaaca ctcccacgtt atacaacatc agatatggga ggattatacg attacgcccc
      961 aacagaagcg ataaaagaaa ggcctcactt agaaaaacat gaagtaaaag taggagaatc
     1021 gtgggaaaaa gggaagtata aagctgcaga taataacatt acttacacag tggcaagcaa
     1081 caaaaagcaa aaagtgacag taccagtata tgcttattta ggtacacagg taaaagtaaa
     1141 cggtaaagtc gtaaatgact cttataaaaa acatggctta gtgaatgtgg atgtaaatgc
     1201 tggagaaaat aagatagaaa ttacaacaaa atatactcca actgctctca tggctttatg
     1261 gatcagtatt ctgacttatt gtttagtggt gtattcaagt tttaaacgat ttgcacctaa
     1321 taagttgaaa gattctcgat atgtgactaa aaaggagata aacaagaaga atgaaaataa
     1381 ataaaaaagt agtaatattg agtggattaa ttatagttag tagtatatat tctttcatct
     1441 ttttcaaaaa tacacagagt atttatacca ctggagatct ttcttttcac ttatctcgaa
     1501 tcaaggggtt atcgactatt tttaccagtc cgattaatta tgagacattc aattatactg
     1561 gatatggtgt aaattatttt tatccctttc tgactttttt tccagctgta atgttatatt
     1621 ggatgacaaa aaatttgatt gtaagctata ttatttacgt ttggttattg aacttgtgta
     1681 caacactcgt agcttatcat tatggcgagc ggtttttaaa acaaaaaaaa gcggcatttt
     1741 tatttagttg tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg
     1801 caattgccga agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa
     1861 tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc
     1921 tagtgtacag ccatgtacta tcaacattaa tgagtacagc attaattatc atttttattt
     1981 ttattcgttt ggtttctaaa ggtttcaaga atgcagattt tattgctata tttaaaaaat
     2041 tatttagtgc ggcaggaatg acattggttc tgacgagtgc tttttggtat ccgatgtttg
     2101 aacaaatgct ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat gctcatgcat
     2161 ctaatgtgtt cgatagtctg actgaagcta tgaacaacga cttaactact tactcaatgg
     2221 gactagttgg tctgttatct ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca
     2281 tagaaaaaaa gatttattat ggtacgtgtt tgacttggct agctacaact agtttggtgc
     2341 catggtatct tctgcaaaat acgccagcca aattgttaca atttccttgg cggatcttaa
     2401 gcttgcaaat aatattctct tctctgatat taacg
//
LOCUS       DAGCXG010000171         2416 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.178, whole genome shotgun sequence.
ACCESSION   DAGCXG010000171 DAGCXG010000000
VERSION     DAGCXG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.178"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(29..274)
                     /locus_tag="MB022_002683"
     CDS             complement(29..274)
                     /locus_tag="MB022_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467642.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(291..1142)
                     /locus_tag="MB022_002684"
     CDS             complement(291..1142)
                     /locus_tag="MB022_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4467643.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1308..2312
                     /locus_tag="MB022_002685"
     CDS             1308..2312
                     /locus_tag="MB022_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBT4467644.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          772 a          410 c          484 g          750 t
ORIGIN      
        1 tatatataca taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta
       61 gttcaggtga ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga
      121 accgtttttt cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt
      181 ggattttctc tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt
      241 ccgagagaga attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg
      301 gtagtatctg ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt
      361 tcttcttctg ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc
      421 actgacttag gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca
      481 atcgtcttgt catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca
      541 gtgcctaatg gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca
      601 gttgtctgta attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct
      661 gtttttgcta gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt
      721 ttacctgctt catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa
      781 ttatcacgaa aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt
      841 tggagcttct tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca
      901 ttccataatt ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct
      961 tggccgacgc tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca
     1021 gccagcgctt ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg
     1081 aatcctacta ccgggatttc gtaaccattt gatagacgat aagtgcttgt taatgaattc
     1141 atactttcag ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa
     1201 gaatccattt tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg
     1261 tatttttgat tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt
     1321 taaaaaaagc aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa
     1381 aagagctgga tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa
     1441 tctcttctgc tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac
     1501 ttaaaatcgc tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg
     1561 atatctacca agaaagtgaa gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat
     1621 tagatacagt gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact
     1681 ttgttgaagc agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag
     1741 gggcgaaaac acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg
     1801 aattaattga aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag
     1861 tgatcgaaag tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag
     1921 ccaagcatct tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag
     1981 aagctgtcta tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa
     2041 aagtcttcaa ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag
     2101 gatttgtccc agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg
     2161 gaaaacaaaa attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc
     2221 gcaacccaga tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca
     2281 aaaatgtgga tgaagtattg aatcgtttat aaaaatgact agaatattag gtgttcaagt
     2341 accagtgatc agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa
     2401 aaaaagagcg tgagac
//
LOCUS       DAGCXG010000172         2398 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGCXG010000172 DAGCXG010000000
VERSION     DAGCXG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.179"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            207..2069
                     /locus_tag="MB022_002686"
     CDS             207..2069
                     /locus_tag="MB022_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4467645.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="MB022_002687"
     CDS             2069..>2398
                     /locus_tag="MB022_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4467646.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAGCXG010000173         2355 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGCXG010000173 DAGCXG010000000
VERSION     DAGCXG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.180"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(51..1598)
                     /locus_tag="MB022_002688"
     CDS             complement(51..1598)
                     /locus_tag="MB022_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBT4467647.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="MB022_002689"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="MB022_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBT4467648.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="MB022_002690"
     CDS             complement(2043..2237)
                     /locus_tag="MB022_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467649.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          662 a          488 c          422 g          783 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttact ttataaaggt cgtccaaaac
     2341 tctatagtgc agata
//
LOCUS       DAGCXG010000174         2351 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGCXG010000174 DAGCXG010000000
VERSION     DAGCXG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.181"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..544
                     /locus_tag="MB022_002691"
     CDS             <1..544
                     /locus_tag="MB022_002691"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4467650.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="MB022_002692"
     CDS             complement(1017..1589)
                     /locus_tag="MB022_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467651.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="MB022_002693"
     CDS             complement(1605..2210)
                     /locus_tag="MB022_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBT4467652.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DAGCXG010000175         2330 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGCXG010000175 DAGCXG010000000
VERSION     DAGCXG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.182"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(94..165)
                     /locus_tag="MB022_002694"
     tRNA            complement(94..165)
                     /locus_tag="MB022_002694"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Glu,seq:ttc)
     gene            complement(177..250)
                     /locus_tag="MB022_002695"
     tRNA            complement(177..250)
                     /locus_tag="MB022_002695"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Ile,seq:gat)
     gene            complement(263..333)
                     /locus_tag="MB022_002696"
     tRNA            complement(263..333)
                     /locus_tag="MB022_002696"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Gly,seq:tcc)
     gene            complement(338..410)
                     /locus_tag="MB022_002697"
     tRNA            complement(338..410)
                     /locus_tag="MB022_002697"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Phe,seq:gaa)
     gene            complement(414..486)
                     /locus_tag="MB022_002698"
     tRNA            complement(414..486)
                     /locus_tag="MB022_002698"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Asp,seq:gtc)
     gene            complement(505..578)
                     /locus_tag="MB022_002699"
     tRNA            complement(505..578)
                     /locus_tag="MB022_002699"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Met,seq:cat)
     gene            complement(591..680)
                     /locus_tag="MB022_002700"
     tRNA            complement(591..680)
                     /locus_tag="MB022_002700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(642..644),aa:Ser,seq:tga)
     gene            complement(703..774)
                     /locus_tag="MB022_002701"
     tRNA            complement(703..774)
                     /locus_tag="MB022_002701"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(739..741),aa:Glu,seq:ttc)
     gene            complement(796..883)
                     /locus_tag="MB022_002702"
     tRNA            complement(796..883)
                     /locus_tag="MB022_002702"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Ser,seq:gct)
     gene            complement(908..981)
                     /locus_tag="MB022_002703"
     tRNA            complement(908..981)
                     /locus_tag="MB022_002703"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Ile,seq:gat)
     gene            complement(995..1065)
                     /locus_tag="MB022_002704"
     tRNA            complement(995..1065)
                     /locus_tag="MB022_002704"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Gly,seq:tcc)
     gene            complement(1067..1142)
                     /locus_tag="MB022_002705"
     tRNA            complement(1067..1142)
                     /locus_tag="MB022_002705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1107..1109),aa:Asp,seq:gtc)
     gene            complement(1161..1234)
                     /locus_tag="MB022_002706"
     tRNA            complement(1161..1234)
                     /locus_tag="MB022_002706"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1198..1200),aa:Met,seq:cat)
     gene            complement(1247..1336)
                     /locus_tag="MB022_002707"
     tRNA            complement(1247..1336)
                     /locus_tag="MB022_002707"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1298..1300),aa:Ser,seq:tga)
     gene            complement(1360..1433)
                     /locus_tag="MB022_002708"
     tRNA            complement(1360..1433)
                     /locus_tag="MB022_002708"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1397..1399),aa:Ile,seq:cat)
     gene            complement(1447..1520)
                     /locus_tag="MB022_002709"
     tRNA            complement(1447..1520)
                     /locus_tag="MB022_002709"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1484..1486),aa:Met,seq:cat)
     gene            complement(1548..1620)
                     /locus_tag="MB022_002710"
     tRNA            complement(1548..1620)
                     /locus_tag="MB022_002710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1585..1587),aa:Ala,seq:tgc)
     gene            complement(1640..1713)
                     /locus_tag="MB022_002711"
     tRNA            complement(1640..1713)
                     /locus_tag="MB022_002711"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1677..1679),aa:Pro,seq:tgg)
     gene            complement(1723..1796)
                     /locus_tag="MB022_002712"
     tRNA            complement(1723..1796)
                     /locus_tag="MB022_002712"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1760..1762),aa:Arg,seq:acg)
     gene            complement(1807..1892)
                     /locus_tag="MB022_002713"
     tRNA            complement(1807..1892)
                     /locus_tag="MB022_002713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1856..1858),aa:Leu,seq:taa)
     gene            complement(1909..1980)
                     /locus_tag="MB022_002714"
     tRNA            complement(1909..1980)
                     /locus_tag="MB022_002714"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1946..1948),aa:Gly,seq:gcc)
     gene            complement(1992..2064)
                     /locus_tag="MB022_002715"
     tRNA            complement(1992..2064)
                     /locus_tag="MB022_002715"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2029..2031),aa:Thr,seq:tgt)
     gene            complement(2082..2163)
                     /locus_tag="MB022_002716"
     tRNA            complement(2082..2163)
                     /locus_tag="MB022_002716"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2127..2129),aa:Leu,seq:tag)
     gene            complement(2171..2243)
                     /locus_tag="MB022_002717"
     tRNA            complement(2171..2243)
                     /locus_tag="MB022_002717"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2208..2210),aa:Lys,seq:ttt)
     gene            complement(2247..2319)
                     /locus_tag="MB022_002718"
     tRNA            complement(2247..2319)
                     /locus_tag="MB022_002718"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2284..2286),aa:Val,seq:tac)
BASE COUNT          636 a          642 c          543 g          509 t
ORIGIN      
        1 cttagcacaa tcatccataa agataactct gctaagcttt ttaaaatcat tctttcagat
       61 aaaaagaaaa agatactcaa aaagtatctt ccatgacccg tacgggattc gaacccgtgt
      121 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaataa aataaatggg
      181 cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta accagctgag
      241 ctataggccc ataaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg
      301 aaggctgtgg ttttaccact aaactacacc cgcataatgg cgcgggacag aatcgaactg
      361 ccgacacatg gagcttcaat ccattgctct accaactgag ctaccgagcc aaacggttcc
      421 gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac
      481 ggaaccaatg tttttagttt ttaattgcgg gagcaggatt tgaacctacg accttcgggt
      541 tatgagcccg acgagctacc agactgctcc atcccgcgat aatatgaaaa aggaggataa
      601 gggattcgaa cccttgcacg gttttacccg cctgacggtt ttcaagaccg ttcccttcag
      661 ccggacttgg gtaatcctcc atgtaaaact atattaaatt aatgacccgt acgggattcg
      721 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
      781 aacaaaatat tgaaacggag aaggagggat ttgaaccctc gcgccggttt cccgacctac
      841 acccttagca ggggcgcctc ttcagccact tgagtacttc cccaaaacca ataagatttt
      901 gtactaatgg gcctaaatgg actcgaacca tcgacctcac gcttatcagg cgtgcgctct
      961 aaccagctga gctataggcc cataaaaaac taaaagcggg tgacgagaat cgaactcgcg
     1021 acaacagctt ggaaggctgt ggttttacca ctaaactaca cccgcatggc ggttccgacg
     1081 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
     1141 ccagtgtttt tagtttttaa ttgcgggagc aggatttgaa cctacgacct tcgggttatg
     1201 agcccgacga gctaccagac tgctccatcc cgcgataata tgaaaaagga ggataaggga
     1261 ttcgaaccct tgcacggttt tacccgcctg acggttttca agaccgttcc cttcagccgg
     1321 acttgggtaa tcctccatat gaaacttaca acgccacaat ggaccttgta ggactcgaac
     1381 ctacgaccgg acggttatga gccgtctgct ctaaccaact gagctaaagg tccaggctct
     1441 taaaaatcgc ggcggagggg atcgaacccc cgacctcccg ggtatgaacc ggacgctcta
     1501 gccagctgag ctacaccgcg aaaaagatat tcattattta aatgaaatgg agcctagcgg
     1561 gatcgaaccg ctgacctcct gcgtgcaaag caggcgctct cccagctgag ctaaggcccc
     1621 gtgtttaaat ttataattat cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa
     1681 accaagtgct ctaccaagct gagctacttc ccgtttaatt aacgcgccca agaggagtcg
     1741 aacccctaac cttttgatcc gtagtcaaac actctatcca gttgagctat gggcgcatat
     1801 ttgaaatgcc gaggaccgga atcgaaccgg tacggtgatc actcaccgca ggattttaag
     1861 tcctgtgcgt ctgccagttc cgccaccccg gcgcgattgc tttggcaaag cggaaaacgg
     1921 ggttcgaacc cgcgaccccc accttggcaa ggtggtgctc taccactgag ctatttccgc
     1981 gtgtttaaag atgccggcta aaggacttga accctcgacc ctctgattac aaatcagatg
     2041 ctctaccaac tgagctaagc cggcatgtaa aacttaaatt atgcgggtga agggacttga
     2101 acccccacgc cgtaaggcgc tagatcctaa atctagtgcg tctgccaatt ccgccacacc
     2161 cgcaaaaata tgagccgtac agggctcgaa cctgtgaccc tctgattaaa agtcagatgc
     2221 tctaccaact gagctaacgg ctcatatgga ggttaacggg atcgaaccgc tgaccctctg
     2281 cttgtaaggc agatgctctc ccagctgagc taaacctcca tggataactt
//
LOCUS       DAGCXG010000176         2307 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGCXG010000176 DAGCXG010000000
VERSION     DAGCXG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.183"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="MB022_002719"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="MB022_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBT4467653.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          763 a          330 c          371 g          843 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatg
//
LOCUS       DAGCXG010000177         2254 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGCXG010000177 DAGCXG010000000
VERSION     DAGCXG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.184"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            103..>2254
                     /gene="acm"
                     /locus_tag="MB022_002720"
     CDS             103..>2254
                     /gene="acm"
                     /locus_tag="MB022_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBT4467654.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQK"
BASE COUNT          884 a          348 c          447 g          575 t
ORIGIN      
        1 agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg agagatgata
       61 tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat
      121 attattttat caatgttatt tattattact aatataacat cactgattcc tgttcatgtc
      181 tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg
      241 aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat
      301 atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa
      361 ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc
      421 agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa
      481 ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg
      541 ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct
      601 tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact
      661 aagcacattc gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt
      721 aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat
      781 caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta
      841 caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact
      901 ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa
      961 aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa
     1021 cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga
     1081 gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt
     1141 ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag
     1201 tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta
     1261 ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt
     1321 tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt
     1381 tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact
     1441 ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt
     1501 cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc
     1561 caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac
     1621 ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat
     1681 gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa
     1741 gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa
     1801 cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac
     1861 agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg
     1921 acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata
     1981 ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt
     2041 agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa
     2101 agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc
     2161 attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg tttattgata
     2221 gtaactatct gtgctgggat attcttacaa aaat
//
LOCUS       DAGCXG010000178         2162 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGCXG010000178 DAGCXG010000000
VERSION     DAGCXG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.185"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..350)
                     /locus_tag="MB022_002721"
     CDS             complement(<1..350)
                     /locus_tag="MB022_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467655.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAVRIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQI"
     gene            664..1086
                     /locus_tag="MB022_002722"
     CDS             664..1086
                     /locus_tag="MB022_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467656.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1096..1299
                     /locus_tag="MB022_002723"
     CDS             1096..1299
                     /locus_tag="MB022_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467657.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1510..2094
                     /locus_tag="MB022_002724"
     CDS             1510..2094
                     /locus_tag="MB022_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467658.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          738 a          323 c          393 g          708 t
ORIGIN      
        1 aaaatttgga tgatcagatc cttaatgaat ttatccagga gagggttgcc aattgcttca
       61 gccatgatcg gtagactggt gatgattagc tgtacgtttt tcttctttag gtaattgact
      121 gaagcgatga tctcgtcgta atttcttcca agtcgatcaa tagcttccac aataaaaatg
      181 tcttgatctc ggacgaaatc caatgcttct tggaaaacag gtcgatgagt aatcgttgcg
      241 cccgattgtt tctcgatgaa aattctcact gcaccgaatt ttttcatttc ttcaatctgg
      301 cgttgttcat tttgatcgat ggatgagaca cgtatgtatg caactctcaa aatttttacc
      361 tactttctgc aactatttaa aatacaccct aatcatacac gataaaacca gatttttcaa
      421 tgttgtggtc gtgtctagtt agggtatacc ttatatagac aaaaactaat gatggaggaa
      481 atagtatgtg tcacttttct ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa
      541 gaaaatttaa acgattatga cattaaaaat atattttatg aattttctag tatataactt
      601 gatttatctg ttaagattta ttaagaaaac gttttcttat ttttgatttc gaggtgatag
      661 acaatgaaaa ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact
      721 ggaactacaa ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat
      781 aaccaagaat taactccttt agataggaac ggaatagcca ttggtaaccc aactaacgac
      841 ttaactgtta ctgaaaatta tttaatagtt tctccattac ttgctcgagc agcttctaaa
      901 acgatacgag tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct
      961 caatcacgga attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta
     1021 caaagttata aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga
     1081 aagtagggat tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat
     1141 attctaagta aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta
     1201 caggagacac aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga
     1261 aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc
     1321 tagtcgacat tctttttttc taattgaaag tgttcacaaa atcacccatt tctggacact
     1381 tccttttttt gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca
     1441 atatctatgt tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga
     1501 gtgatttttt tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta
     1561 caagaagaca gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg
     1621 gcaaaaagta atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca
     1681 cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc
     1741 aatcgactga atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac
     1801 aaatcaagtt ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag
     1861 cgtaatttga tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg
     1921 tttggtggtc gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg
     1981 gatagcggta cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt
     2041 tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg
     2101 tcatttaatg ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt
     2161 tt
//
LOCUS       DAGCXG010000179         2158 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.186, whole genome shotgun sequence.
ACCESSION   DAGCXG010000179 DAGCXG010000000
VERSION     DAGCXG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.186"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(143..418)
                     /locus_tag="MB022_002725"
     CDS             complement(143..418)
                     /locus_tag="MB022_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBT4467659.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(661..1971)
                     /gene="der"
                     /locus_tag="MB022_002726"
     CDS             complement(661..1971)
                     /gene="der"
                     /locus_tag="MB022_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBT4467660.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT          629 a          468 c          355 g          706 t
ORIGIN      
        1 ataaaaatta aataaaatac atgtgttttc tatgtatgac atacggattc ggaatctctg
       61 tctcttattt attccttttc tgctaggaaa taaaaagaaa agcacttgta gccctagagc
      121 cacaagcgct ttttcaactg tcttatttaa cagcgtcttt taatgcttta cctggtttga
      181 atgcaggtac tttgctagct ggaatttcga tttcttcacc agtttgtggg ttacgacctt
      241 tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc gattaattga actttttcgc
      301 cgttagctaa agcatcttga atagttgaaa atacagcatc tactgctgca gttgcatctt
      361 ttttagttaa atcagtagct gaagcaactt tttcgattaa ttctgctttg tttgccatgg
      421 atagattcac ctcctccaca agagatatga tagaaatatc atttagttaa aactttctga
      481 gtggtccaga agtttaaaaa gaaatattga ttaacttcaa tattctgtta tcacgatatc
      541 atatgaaccg cttaatagca aggtttttac caattttcac atgctttttt tagtcataga
      601 aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc
      661 ctattttctc ctacgaggga taattcggat ggacgtccct tcaaaagtaa atgcttttct
      721 gatttgattt tctaagaaac gttcataaga aaaatgcatc aattcctctt cattgacaaa
      781 aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt
      841 tcctttatct gttggtgttg gattgattgc tacagcatcc ataatgatat cattcaacac
      901 agccgaagag atacgaagat tctggttcat acttacttgt tcgatcattt caggcagacg
      961 ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat caagataccg
     1021 gaattcatca cgaatctctt gttcgaaatc acgcattgta ttggtttctt tctttacaag
     1081 atcccattta ttcacgacga taataattcc acgaccagct tcatgggcgt aacccgctac
     1141 tcgcttgtct tgttcccgaa tcccttcttc agcattcagt accatcaaaa cgatatcaga
     1201 acggtcaatg gcacgcattg cacgcatcac gctgtatttt tcagtatttt cgtatacttt
     1261 tccgcgttta cgcataccag ctgtatctat cattaagaat ttctgaccac tttcagaaac
     1321 gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc
     1381 ttctcctaaa atcgcattga tcaaagagga tttaccaaca tttgggcgac caatcaaact
     1441 aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca gagaaatact tcactgcttc
     1501 atctaaaata tcaccaatcc ctagtccatg gcttcctgaa acaggataag gatcgcctag
     1561 ccctaaggaa taaaattcat agatatcgtt tctcatttct ggattatcta ctttattgac
     1621 tgctaaaatc actggtttat tacttctgta taaaattttc gcaaccatct catctgcgtc
     1681 tgtcacgcct tcgcgtccgc tgactacaca aatgattaca tctgcttctt caatcgcgat
     1741 ttctgcttga tgcttgatct gatccataaa aggttcatcg ccaagatcaa ttccgcctgt
     1801 gtcgatcacg ctgaattctc gacctaacca ttcacctttt gcataaatac gatcacgagt
     1861 tactccagga gtatcttcga caatcgagat acgctcacca gcgatgcggt taaaaatcgt
     1921 agacttaccg acgttcgggc ggccaacgat tgcaattgtt ggatttgcca tattatttcc
     1981 tcctcacaag tttccaatct cacttagttt aacgaatgca ttgccaactt tcaagcatag
     2041 attgtcaatt aaacgaagta tttttattcc ttattgaaat actaatcgct tttcaaaaga
     2101 aaaactgaga caaaagcagc tgctttcatc tcagtcacag atacaaaatc aattattt
//
LOCUS       DAGCXG010000180         2104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGCXG010000180 DAGCXG010000000
VERSION     DAGCXG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.187"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            169..1539
                     /locus_tag="MB022_002727"
     CDS             169..1539
                     /locus_tag="MB022_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HBT4467661.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="MB022_002728"
     CDS             1560..1844
                     /locus_tag="MB022_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467662.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DAGCXG010000181         2100 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGCXG010000181 DAGCXG010000000
VERSION     DAGCXG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.188"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            82..1029
                     /locus_tag="MB022_002729"
     CDS             82..1029
                     /locus_tag="MB022_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4467663.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            1159..1899
                     /locus_tag="MB022_002730"
     CDS             1159..1899
                     /locus_tag="MB022_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467664.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          740 a          343 c          437 g          580 t
ORIGIN      
        1 ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa
       61 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
      121 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
      181 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
      241 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
      301 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
      361 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
      421 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
      481 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
      541 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
      601 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
      661 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
      721 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
      781 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
      841 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
      901 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
      961 gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca
     1021 acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt ttcttaaaat
     1081 aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa agttccattt
     1141 ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt tcctgcgtac
     1201 gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt tgacggacaa
     1261 gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga atttcatttc
     1321 acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca
     1381 gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta taccggccat
     1441 taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc ctctcgttct
     1501 gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc atctgttgac
     1561 acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc gattatccca
     1621 gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa aaaaacggat
     1681 gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag aaatggtgag
     1741 ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa
     1801 aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt gatcgatgat
     1861 caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa atttatattc
     1921 ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta tccgaaagtt
     1981 tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa taagtaagaa
     2041 aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa atagtttatt
//
LOCUS       DAGCXG010000182         2090 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGCXG010000182 DAGCXG010000000
VERSION     DAGCXG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.189"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..1285
                     /locus_tag="MB022_002731"
     CDS             <1..1285
                     /locus_tag="MB022_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467665.1"
                     /translation="ISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVK
                     GEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGV
                     NDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDAD
                     NEKGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQD
                     ATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGS
                     LIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFL
                     AQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFM
                     IYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(1571..>2090)
                     /locus_tag="MB022_002732"
     CDS             complement(1571..>2090)
                     /locus_tag="MB022_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467666.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          757 a          309 c          378 g          646 t
ORIGIN      
        1 cattagttta tctattaaag atgctacgga cagagcttca aaagaaacat ttgctaatat
       61 aacaaatagt ttttctatgg agataaatag acaagtaaat ccaggaacac ctagaggtgg
      121 gggaaatgta aaaggtgaag atattaaaaa gatatctcaa acagatagta tagactccta
      181 tgtaaaaaga ataaacagtg ttgcagattt agttgattat gatattattg aaactcaaga
      241 gactcttgcg aatcaatcac ctgaaagagc aaagaatttt aaaagaacag ttatgttaac
      301 tggggttaac gactcaacaa aagaaacaaa atttgtatca gaagcatata aattagtaga
      361 aggaaagcat ttagaaaatg aagataaaaa taaaatctta atgcataaag atttagctga
      421 aaaaaataac cttaaagttg gagataagat aaaaataaaa tctaatttat ttgatgctga
      481 taatgagaaa ggtgcagatg aaacagtaga agtagaaatt aaaggtctat tcgatgggca
      541 taatagcggt gtagtaagtg cagcacaaga actatacgaa aatacattaa tcactgatat
      601 tcatacagct gctaaagttt atggtaatac cgaagataca gctgtatacc aagatgcaac
      661 attctttgta aaaggtgata agaatcttga tagtgtaata aaagatcttg ggaaattaga
      721 tataaattgg agagaatata atttgattaa aagttcatca aattaccctg cattacaaca
      781 atctatatca ggtatttact caatttcaaa taaattattt tttggctcat taatatttgc
      841 aggagttgta gtttcattat tattattctt atggatgaat gctagaaaga aagaaatagc
      901 agtattacta tcattaggta tatcaaaatt agaaattttt ggtcaattcc taattgagat
      961 ggtatttata tcaatccccg cattcgttgg ttcctatttc ttagctcaat atacagctga
     1021 caagcttggg aataatatat tgaataaggt tactggtgat atagctaaac aaatagctaa
     1081 acaatctgca tctagccaat taggtggtgg ggcagaagct gaaggattta acaagacatt
     1141 atcaagttta gatatcaatg tattacctaa attcatgatt tatgtagtta tatttatgag
     1201 tttagtactt cttgtatcat tgattatttc ttcattcaat atattaagaa ggaatccaaa
     1261 agaattatta attgacaata attaaaattt tcggtgcttt ttagcaccaa aaatgagagt
     1321 gtaatataaa ccgtgtaagt aaccatcaat cctaggcggg tggttgcttc acggttttta
     1381 tttttctaaa ataagatcaa gaggtgatta cacgatggct agaaagaaaa gaaatcctga
     1441 tgctgagaag ttagctgaat ccattttgaa tacttatcaa cccgaatctg tagaggatat
     1501 gcaagatgtt ttaaaagatg tgtttggtcc cttatttgaa aaaatgcttc aaggagaatt
     1561 gaataaccat ttagggtatg acgcccattc taaagagcca aagaaacatg acaatcgacg
     1621 aaacggttat ggcaataaga cgcttaaaac gagctttggt aaagtaaaca ttgatgttcc
     1681 cagagaccgg gaggcttctt ttgaacctga gttgattcct aatagaaaac gagatgtctc
     1741 cgacatcgca gactgtagac aaaagctgtt ccaaactcgt aactcgagct tggaacagct
     1801 ttttatctac ttcgtttcga atttttgaac aaaaaaataa aggctccttg cctttccata
     1861 gccaggtggc taatttcttt aaattcattg cagcaaacac tagcatcgta tgcgtcgcga
     1921 ctttttccaa gcctcgatac ttggtccaac gcatcccatg cttttctttt gcatccgcaa
     1981 atattcgttc aatcgtttgt ttccgttttt tgtaaattga tttattcaga tctgttaggc
     2041 gaagatgttc cacttcatcc atcagatctc cccaaacatg tctcgtgatg
//
LOCUS       DAGCXG010000183         2076 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGCXG010000183 DAGCXG010000000
VERSION     DAGCXG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.190"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..447
                     /locus_tag="MB022_002733"
     CDS             <1..447
                     /locus_tag="MB022_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBT4467667.1"
                     /translation="YRPPIKIDLPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYR
                     KALEFLIKDYAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHY
                     VRRHTNKDVHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(593..1069)
                     /locus_tag="MB022_002734"
     CDS             complement(593..1069)
                     /locus_tag="MB022_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBT4467668.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(1029..1376)
                     /locus_tag="MB022_002735"
     CDS             complement(1029..1376)
                     /locus_tag="MB022_002735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467669.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(1363..1548)
                     /locus_tag="MB022_002736"
     CDS             complement(1363..1548)
                     /locus_tag="MB022_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467670.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1549..1821)
                     /locus_tag="MB022_002737"
     CDS             complement(1549..1821)
                     /locus_tag="MB022_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBT4467671.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1818..2021)
                     /locus_tag="MB022_002738"
     CDS             complement(1818..2021)
                     /locus_tag="MB022_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467672.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT          622 a          396 c          297 g          761 t
ORIGIN      
        1 taccgtccgc cgattaaaat agatttacca gaaaatattg aaaaagtttc tcctacattt
       61 gttgatattt atactcaagc cacacaagct aaatctgagc aattggaaca aattgctgga
      121 gttggttata gaaaagctct agaatttcta attaaagatt atgcaatatc taaaaattca
      181 gaggatgaag ataaaataaa aaaaatgtta ttaggtaaaa caattgaaaa ttatttgaat
      241 gattttccta aactccaaag attagccaaa gctgctactt ggattggtaa tgatgaaacg
      301 cactatgttc gtagacatac taataaagat gttcatgata tggatgcatt tattaaatca
      361 gcagcgcaat ttatcgcggc tgattatgat gctgatttag ctgatcaatt tataaatgga
      421 aataacttta tgccaactac taattaaaag aataaaagag ccacagatac caattaataa
      481 aacggttggg atatcttggc tctttcatat ttttttgaca ctaatagaat atcatgctgc
      541 taacaatgtt tacatagcta caatgaccga acatttagtg aacagtgtaa ttttagaata
      601 aagagagttg ttctttgtac ttctcgctat tgtaaaaatc aatttcgttt tcttttttca
      661 atcggtcagc ttgtctgtgt tcatactcat ctaaaaagtt caatgtcttt ctaatttctg
      721 catgtcgctg tctgacatac gatgagctat acccaacaga ttcagcaata tcttctaagc
      781 tcatttgatc aacatatttc atcttaacaa tttgattatc gataccagaa aagctatcta
      841 tcagcaataa catttcttct ttttgctcga tcaacaattc caattcattt tctatctttt
      901 ggatattctc ttctaaagaa gaagttctcg aattcttttc gatgcggacg tttgctaaat
      961 caccacagac ccatcgattc aattcaagct tacttttatt aagattccac tttaagtaaa
     1021 gaatctgctc atctagttct tggtaatctt ttaaccaatg aaatctcaca aacgccaccc
     1081 cttatggtag aatagtattg cgctgctatc tcgaaagggg tagctttttt attttttctt
     1141 agccgttcca ttctggattg ccttttgttc caaacgtcgt tttttcttct taatctttga
     1201 ttttgttttt cccatacaat tctcctttcc gaaaacacat tttgttcgat ttttaatact
     1261 aaataaaagt tggccagaat ttttcgttcg tctttactca tctttttttg aataagatta
     1321 ctagcataaa aatagttagt accacgaaaa tgaacaggta aatcaattac gttcaccttc
     1381 ttcatgccag ttatcagcca ctagaatctt tgcgttagtg ccgccaccgt tttctttttt
     1441 gtatttttca aatgctttat aagcagctgt ttttgattta tagatttttc cggaaccgac
     1501 tgggaacatc catgtgccct ctgtgatagc taagatgtat actttcattt aattcacccc
     1561 tttaatcccc aacactacat atccggactt ctgcatgtaa tcagtaatat aagtgatctc
     1621 tgctttgtat gagtcaccag tgtaactaaa tgttggtctc gttagtccag gtaattcatg
     1681 tttaatgtat tttttatatt cacgtagtat taactgatca ccaactttga agtcacgatc
     1741 attcttgcgg atctcgaatt gtttgcgtcc gctcgtcact gcttcaaaat attctggtag
     1801 tatttttagt tcgtggatca ttctgtttcc tccttcaaaa ttcgctcatc atcaaaaagt
     1861 gaatctccac aaaatccttc tcccaaatat ttatcgttca tgtataagtc aaccaaatat
     1921 ttgtgatctg cttctcgata ttcattaata ttaacgatct ttattttgta aacattatct
     1981 tcgtcgctca ctatcttgaa ttgttctctg actttcacca ccgttcctcc tctttttgaa
     2041 aaaattttag tgtttttgta agacccccta aatcag
//
LOCUS       DAGCXG010000184         2072 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGCXG010000184 DAGCXG010000000
VERSION     DAGCXG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.191"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..195)
                     /locus_tag="MB022_002739"
     CDS             complement(<1..195)
                     /locus_tag="MB022_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467673.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
     gene            869..1681
                     /locus_tag="MB022_002740"
     CDS             869..1681
                     /locus_tag="MB022_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467674.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          683 a          297 c          339 g          753 t
ORIGIN      
        1 ataatagcca tttcgttgac tttgtcggtt ttctgttcgt tcatattctt ttgcttgaat
       61 atattctgtt cgttgatttt ccattagttg attaaatacc gttgttaaaa tatttttaga
      121 aacgtcatcc tttacagaat attcaataat gctttgaatc tcttcgcttt tcagtgtaaa
      181 atgtacttgg gtcatgtaaa agtcctcctg ggtatgtttt tgtcgttaaa aacattgtac
      241 cgtaaaagga ctgttacatg gcctttttac ttttacacaa ttataaggac tttatcgaaa
      301 aaaagcagtt atttgttaaa atcattgata ttattaataa taagtatttt ttgaaagggg
      361 aaatataaaa ggtttttgaa aataaataaa aaatagtatt acttgttcta ttgctagtta
      421 ttgctagtct ttattctttt ttgtttttta gggatgtaca aaagatctca accacaggag
      481 acattgcttt tcacttttct agaataaaat gcttgtcctc agtattttct ggtccaataa
      541 attttaccac atttaatagc tatggcggag gcgtgaattt ttttatccat atctaacatc
      601 tttccagccg taatatttta ctggacaaca aacaatttga ttctaagtta tatacttact
      661 atacgttggg atactgaata tctgtacaat tctagtgagc tatcaatatg gacaaaagaa
      721 tagatgcagc cttcttattc agctgtttgt atagctttta tggattgata tatattagcg
      781 tactgcaatt tcagaatcaa ttgcgttgac gattgtacca gttgtcttgt attacacata
      841 tactcttctt tatgaaagaa aaaactgcgt gattctttta gctgtcagta tgagtttgct
      901 catctatacc catattctct ccacctttat gagtgtgatt gttattggct tcttgtttat
      961 cggctctctt ttctctaagc ggaaagagag aaaagaaatg tcgaatctga tatcaaatat
     1021 gataaaagct gttggttgga ccattctttt aacagcttac gtttggtatc cgatgttaca
     1081 acaagtccta catcaaaaaa ttaatcgtcc ttttcgcaca aaacttcaag aaagggcatt
     1141 aaacatttct gatagtttaa tcggtgcagt aaataatgat ttagttacat ttacaatggg
     1201 aacaattggt gtggtttcat ttattcttcc acttttcttt aaaaaaaaat ttgccaataa
     1261 agaaaagatt attaattttt gtgcagtgac cacttggttc ttatcaacga atctttttcc
     1321 atgggaattt ttacaaaaaa cacctattca aatcattcag tttccttgga gaatcttggg
     1381 ttttcaagtg ctttttggtt cattgatctt aattatagtg tttttaaagt ggaaaacaag
     1441 caacaaaaaa agtatgtgta gccttgtagg aatagtcctt cttattttta cagtgactgt
     1501 agcaacagaa gcgaattatt cgcagaaaat tcaatcgtat aaaggtcgtt taattatgac
     1561 caaaaaagat gttacacttt atacaacctc gagaactgga ggtctttatg actatgcacc
     1621 attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag tctatgattg
     1681 atgggagaat gcatctcata aagcacataa ttctaaaata atctatacag taggagatgc
     1741 gaatgagcaa aatatcacgt tacctgtatt taattactgg gaacagttat gaaagtaaat
     1801 gggaaaaaga gcactataga aaatgacaat ggtttagtgt ctattaaagg tacgggaaaa
     1861 agaactatag ttgaggtttc atctacgtat ccaactacga tgattctatc tttattaatt
     1921 agttcaataa cttttatatt cttatttaca cgttatttaa taagatgctg cggttaaatg
     1981 aaaatagatt agatagatat tataaatatg ttgattctat tttcatagca atggagaaaa
     2041 tacattagca atatataaaa gaaggtatca ag
//
LOCUS       DAGCXG010000185         2066 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGCXG010000185 DAGCXG010000000
VERSION     DAGCXG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.192"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(495..839)
                     /locus_tag="MB022_002741"
     CDS             complement(495..839)
                     /locus_tag="MB022_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467675.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(936..1181)
                     /locus_tag="MB022_002742"
     CDS             complement(936..1181)
                     /locus_tag="MB022_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4467676.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1251..1505)
                     /locus_tag="MB022_002743"
     CDS             complement(1251..1505)
                     /locus_tag="MB022_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467677.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1669..1992)
                     /locus_tag="MB022_002744"
     CDS             complement(1669..1992)
                     /locus_tag="MB022_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467678.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          779 a          355 c          359 g          573 t
ORIGIN      
        1 tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact
       61 ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat
      121 gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca
      181 gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa
      241 tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg
      301 cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac
      361 caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg
      421 acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg
      481 cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct
      541 gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa
      601 tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg
      661 ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa
      721 ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt
      781 aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat
      841 ttttcatgcc ataatcacat agaaatgtct cctttatata tagattgtta accttagacg
      901 ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct
      961 gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca
     1021 atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt
     1081 aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga
     1141 caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata
     1201 gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt
     1261 ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat
     1321 tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag
     1381 tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga
     1441 taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta
     1501 atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat
     1561 cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga
     1621 acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa
     1681 aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac
     1741 taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt
     1801 ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt
     1861 aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg
     1921 gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg gcaaaataat
     1981 cactcctaac attgagaata aataaaagaa taacattttt tctaaaataa gcaaataaaa
     2041 attaaagtaa caaaatagtt atgaca
//
LOCUS       DAGCXG010000186         2018 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGCXG010000186 DAGCXG010000000
VERSION     DAGCXG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.193"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            100..570
                     /locus_tag="MB022_002745"
     CDS             100..570
                     /locus_tag="MB022_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBT4467679.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="MB022_002746"
     CDS             792..1895
                     /locus_tag="MB022_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4467680.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          327 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcata
//
LOCUS       DAGCXG010000187         1996 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGCXG010000187 DAGCXG010000000
VERSION     DAGCXG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.194"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..956
                     /locus_tag="MB022_002747"
     CDS             <1..956
                     /locus_tag="MB022_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBT4467681.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALMASMLEMYVSGVST
                     RKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1013..1405
                     /locus_tag="MB022_002748"
     CDS             1013..1405
                     /locus_tag="MB022_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467682.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1406..>1996
                     /gene="aac(6')-Ie"
                     /locus_tag="MB022_002749"
     CDS             1406..>1996
                     /gene="aac(6')-Ie"
                     /locus_tag="MB022_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HBT4467683.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYF"
BASE COUNT          756 a          270 c          360 g          610 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaacaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc tatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      421 caacattttt tgaataccta aaagaacgcg gtttacaagg tacggaactc gttatttctg
      481 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgta agttggcaaa
      541 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      601 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg
      661 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
      721 cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc
      781 gactaaagag taccaatcta attgaacgac tgaatcaaga agtacgcaga agagaaaaga
      841 ttattcgcat cttccccaat caaacatcag ccaatcgctt aattggagcc gttcttatgg
      901 acctacatga tgaatggatt tattcttcaa gaaaatacat caattttgat aagtagaaat
      961 ggtaaaaaca ttgtatagca ttttacacag gagtctggac ttgactgagt ttatggaaga
     1021 agttttaatt gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga
     1081 taccagttca tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg
     1141 ctatacactt ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt
     1201 acctaactat caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc
     1261 taaagagatt ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg
     1321 ccatgtatat gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata
     1381 tgattatgaa aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac
     1441 tttaatagat gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga
     1501 attttatggt ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga
     1561 gccttgggaa gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata
     1621 tggacaaata tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga
     1681 tgagatagtc tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat
     1741 tggtacaaga tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc
     1801 agttatttta gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg
     1861 ttttagaatt attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg
     1921 ttatttaatg gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt
     1981 aattgagcat tacttt
//
LOCUS       DAGCXG010000188         1980 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGCXG010000188 DAGCXG010000000
VERSION     DAGCXG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.195"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(350..805)
                     /gene="tnpA"
                     /locus_tag="MB022_002750"
     CDS             complement(350..805)
                     /gene="tnpA"
                     /locus_tag="MB022_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBT4467684.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYRRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1048..1980
                     /locus_tag="MB022_002751"
                     /pseudo
     CDS             1048..1980
                     /locus_tag="MB022_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          630 a          393 c          371 g          586 t
ORIGIN      
        1 acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga taatccagtg
       61 attttctagg aagattattt ctgcgccagc attttctaat gtttgaattt gtgttttgta
      121 atcttggttg cttgtgctaa ttcttgaata gccgtaaatt gccaaaattt catcaccctt
      181 tctggacata agtttctgac accagttaaa atcaagtcta tcagtaaccc atacacccgt
      241 gaggatgggt gtatttttaa tccccctaga aggggtcgac actcgctgcc ccttaaaagg
      301 ggatttttaa aagaccgcca actgctcatc ttcactcact tacttctctt tattttttaa
      361 atggttctac gtactctctt ttacttataa tatatcgaat gcgatcatca gcttcttgtt
      421 cacggatgta tttttcattt gtcttttgat tcaatccgac tgtactcaca tagtatctct
      481 tcgaccaaaa ggatttgttt ccgtatttat atttgagatt tgcgtgctgc tcgtggatta
      541 gtaccgcact acgacctttc agatacccca taaaactgga tatacatatt tttggtggta
      601 ctcgaacaag catgtgaata tgatctagca ttgcatgtgc ttctatgatt tccacatctt
      661 tcatttcgca caacttccta agtatttttc caagatcttt tcgtaattgc ccatagatta
      721 cttttcgtcg atattttggt gtaaaaataa tatgatactt acaattccaa cgagtatatg
      781 ctaaactttt atcgtcattt gacatgaaaa aactcctttc gatctgatga gcggttggta
      841 gccactcact agtatatcga aaggagtttt tatcgctaaa gatacttctt ccacgcgtag
      901 aaacgcgcgg ttttcttgtt tcgcacgctt tacgcacaaa actgactgaa gccattaaga
      961 aaataaaagg gtctaaaact tttaagtttt gtaccgaccc ccaaaagtta gaccaaaaaa
     1021 tctaactttt ggaggtcggt attttttata gctaagtata cttttgaact taaattaaag
     1081 atcgttcatg attatttgga tggtaaaggc ggaagtgatt atctggcaaa gaaacattcc
     1141 attaaggttc catcacaagt gaagcgctgg atcaatgcct atcaagtatt tggtgaagag
     1201 ggtctagtaa gaaagcgaca aaagaaaaaa tattctgttc aatttaagct ggatgcgata
     1261 gagttatatc taacaagtga gctgtcctat cgtgaagttg ccaacacact taacatgaac
     1321 aaccctaatt tgatcgctag ttggatgcat caattccgtg agggtgggat cgatggactc
     1381 tcaaaaacga aaggatgtcc acctatcttg tctaagaaat atgaacccaa aaataagaaa
     1441 aaatctacaa ctaaagcaac gtctaaagaa cgtgaacgca tcgaggagtt ggagaaacga
     1501 gtccgttccc ttcaaattga aaatgcctct ttaaaagaat tgaggaagct gcgaaaacag
     1561 gaagcccaac aacgacgaat gaagcaatcg cacgaatcat cgcaagcctc cgaggaccgt
     1621 ttaaattagt tgagcttcta gaaactttaa agttccctaa ggcaaccttt atgtactggc
     1681 aaaaacgatt ggaccgtaaa aatccaggtc aagagattga agcggaaatg cttaaagttc
     1741 gggagaagca caaggattat ggctgtttac ggatgaccaa tgaactaaga aaccgcgggt
     1801 tctctatcaa taaaaagaaa gttcagcgcc tgatcaagaa gctaggaata aaagtaacga
     1861 catacacacg taaatcacgg cgctacaatt cttatcgagg acaagtcgga gcagtagcca
     1921 aaaatcggat ccatcgtcgt ttctacacca gtatctgcca tcagaaaatc acgacggata
//
LOCUS       DAGCXG010000189         1980 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGCXG010000189 DAGCXG010000000
VERSION     DAGCXG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.196"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            593..925
                     /locus_tag="MB022_002752"
     CDS             593..925
                     /locus_tag="MB022_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBT4467685.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1544..1885
                     /locus_tag="MB022_002753"
     CDS             1544..1885
                     /locus_tag="MB022_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBT4467686.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          689 a          266 c          421 g          604 t
ORIGIN      
        1 ttgagttata ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt
       61 gatattactt cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta
      121 attgtctgct atgcttgtga tgaattgatt ttcgttctgc acttttggat gataaatttg
      181 gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg
      241 tcagcgttca tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg
      301 gtattacccg caatggattt agtataacag atattcaaaa gtgataccac tttttttggc
      361 tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca caatgttata
      421 aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg aacgcatgtt
      481 tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta tttttgaaaa
      541 ataacaagaa aaacttgtta aaaatattta aagaaattgg gtggtttatt ttatgtcata
      601 tacaggaatt ttgagtctca aagatatttg tcactacggt aaacggtgta ccgctacaga
      661 gaaaatcacg aagaaactat caactggcca aagtaaaacc gttgtgcagt gtaagaaata
      721 tataatccag aaagacaaag tctcagagga aatgatttat tacattggga aacaaaagca
      781 aataatttta aaagatccta tccccttaaa agagctatat ccaacaatca aacatgttta
      841 tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa
      901 agggatgggg cgtttgatcg gctgaattga tcattctcat aacttgtatt ttatcgtttt
      961 tcgcttgctt gaaaatattg tcgagacttc taaaggaact tagagtcact agaaaaataa
     1021 ggagtagact tgatctatca acaattccct tcggggagtg acggggtagg ttttaacgat
     1081 tggtaacgta aaaaattggt caatcgagta aatgcgcttg tttggatagt ggtgaaatga
     1141 atttgtgtaa ataataagcg cagagtaaac aagagggaat ttagaagtta gacaagttag
     1201 aatagcgtct agattaatca tgagcagcta ctaagggatc tagactttga cgcaaactca
     1261 tggaataagg agtaggtctt ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc
     1321 tgcagtacat gtagatactt gcgaggacgt tgattattgg atcaatcgag catttaccgc
     1381 tgtctactag ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt tatgtatttt
     1441 taagtaaacg aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa aaattgatat
     1501 aaaacataga gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa attatgatcc
     1561 gaatatacga ttgggcacac atacgattaa ggtttctttc caacgttggg attacaaagg
     1621 atttgtgacg tttagaagag gcggaaattg taaaggatta gatgttttag cattagacga
     1681 ggatgattta tacgatcaaa cattgactga taatccaatt ggattcggct tgctacctga
     1741 agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt
     1801 atctgttgaa gatacttggt catatttgag tgattatatt gtcagctttg aaatcattga
     1861 atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta
     1921 gaaggagtca cgatgattaa taaaaaggta gtattacctt aggcattgag taaggaattt
//
LOCUS       DAGCXG010000190         1889 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGCXG010000190 DAGCXG010000000
VERSION     DAGCXG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.197"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            60..296
                     /locus_tag="MB022_002754"
     CDS             60..296
                     /locus_tag="MB022_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBT4467687.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            315..530
                     /locus_tag="MB022_002755"
     CDS             315..530
                     /locus_tag="MB022_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBT4467688.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1233..>1889)
                     /locus_tag="MB022_002756"
     CDS             complement(1233..>1889)
                     /locus_tag="MB022_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4467689.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
BASE COUNT          612 a          295 c          339 g          643 t
ORIGIN      
        1 tactgttaat caaatacgta tgttacagta gcaccgtaaa aaatattatt taaagaagcg
       61 tgtttaaaca tgcaaaatgt aaaggaacta agtattgtgg aaatgcacaa actgttggtg
      121 gaggcaataa tgcttgggga aaattggggc aagttgtagg tggacttacc acaggggcag
      181 taggtggtgc aggtttagga acagcaattt gcggaccagc ttgcggtgtt gttggagggt
      241 tatatggagc tgtcgctgga ggtgctgcag ctggctggga tgctcgaaaa aaataaaaaa
      301 ggagaataag tagtatgcat attaaaaaca caaaaacaac atttatatta tcttcagaag
      361 agttgaaaaa tattcaaggt gggagtgccg tgggagtttt aggcactact ttttctgggg
      421 caacagcagg agtaaaactt tgttcagccg gaggacctta tgcaatagct gcttgtggtg
      481 taggtggagc tttattaggt gctggattca gcatgtggac cggtgcgtaa acaattaaaa
      541 aatatcaaac ctcaagattg gtttgtgtcg attacgaaca taggtatgtt ctttatcttt
      601 tcaaaagttt ggaatggaca gaacttttta ctaaagcttc ttgtgctagt ttttataatt
      661 atattaaatt tttatctttt attaaagtca gataaaaatt ctttggaaat gtaatggtag
      721 agatattttt acaaaaggac ttgcagcgct gtagttggtg ctgcaggagg acctatttcg
      781 gccgttacag gaggtattgg taatgtactt gtcggatgtg cattaaagcc tgttcctgca
      841 agttaaattt ataaaaacag gaaagtaatt atctcaaatc ctatcaagta caatcatagg
      901 attgcacttg acaggacaaa ttttttaact aaaaaatgaa aaacactttc taaaaattag
      961 tagaatttgc caaatctttc gcatcttcca ccatacattt aattttaatt cccctttatg
     1021 gtacttaata aaaacaagaa aagaactatt catttggccc ttttttggtt ccagaccata
     1081 gaaatattcc tccagtaact aatattgctg ttatgcacgt aactaataac agctataaaa
     1141 attggtacaa ctttaatagg attcagaaaa atttgcacta gtgaaggaat ctattttatt
     1201 agaaaaatgc aatctcccta aagaaaaaac cactaaacag tgctttcttt caacttctcc
     1261 ttaatttttt gtgctttccc taaagtaaag tttctggcca actctgaatc aaaatatagt
     1321 gaacgttttt taaaatctat ttctttaacg ttaaagatat ttactaaaca cgctcgacta
     1381 attcttgtta acataggata tttcttttca agctcactaa ttcttccata aaattcgtac
     1441 tgcccatctt tagtacacaa tgataatcta tgtggtaatt ttgaagcttc tacaaaataa
     1501 atgtcattta tatcaaaagt aaatgtttga gaacctatgg aaaaaacaaa tgcttgattc
     1561 gtaacctgtt ttgttgcatc tatcctttct tgcgctaata acaataactc aacaatctcg
     1621 tttcgatact catctagtgt ttgatctttt gttacaaatc ctaacgtttc aactcttctt
     1681 tttattgtta cgggtaacat ctcatcatga gtagttgtaa aaatgatttt tgcttgaaca
     1741 tcatattttc tgattacttc tgctaattct ataccgttaa cttcatgatt taaatcaata
     1801 tctaagaaat atattccttg ctttggtcgg aattgtttga ggtatttttt aacttctaac
     1861 ggactttgtg ttttcaatac tatcttaaa
//
LOCUS       DAGCXG010000191         1843 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGCXG010000191 DAGCXG010000000
VERSION     DAGCXG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.198"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(345..418)
                     /locus_tag="MB022_002757"
     tRNA            complement(345..418)
                     /locus_tag="MB022_002757"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Ile,seq:cat)
     gene            complement(607..1626)
                     /locus_tag="MB022_002758"
     CDS             complement(607..1626)
                     /locus_tag="MB022_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4467690.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1623..>1843)
                     /locus_tag="MB022_002759"
     CDS             complement(1623..>1843)
                     /locus_tag="MB022_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4467691.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          549 a          371 c          321 g          602 t
ORIGIN      
        1 taagcctttt ttctcacttt ttttcaaaaa tatggtatgc cttttaatag cttcaaatat
       61 aaaagagttt aaagcgtaac acacttatgg ggaagtggtt tgaggtgcgc tttaaactct
      121 tctttattat tatctcacaa tttaacccaa atgtctttct atttaaaaat caaagtaaaa
      181 cttttcaaat atacagaaat ataactatgt gaaacatcct ttcattaatc cataaaagga
      241 tacataaaaa agccactcat ttgagtggca atgaagaaaa gctttagctt gtataatact
      301 cttcaaaaaa ttctaacaca gaacgattca aatggctacg ttaatgtacc ctgtaggact
      361 cgaacctacg accggacggt tatgagccgt ctgctctgac caactgagct aagggtactg
      421 gttgttgcca cataaagcca taaacaatca accagtagaa tgtgtggcaa caaacctgtt
      481 atcgcatagc ttggagtgtg actattatgg gtgatagtga agatatgcga taacatcact
      541 attttatcga atgattttta tagttgtcaa tatagttatg taatgctcct caacgaggag
      601 ctatttttat cgtttaggaa tatttaaata ccaacgtttg tcatggaaat cttgcgcacc
      661 gcctttagtg tttccctctg gatcattcgt tgcccgcatc atgacataga ctttcttatt
      721 agggaagtta cgcatgttaa aagatacatg atagccaaca tttccagaag tattataagc
      781 ttgatttaca tctgatctat aaattccatc agctcttact cgagctaatt ctttcccagt
      841 attgtaatcc ataatgaaga tatactcgta tttatagtta gcaatgtgcc atccagctac
      901 atgcaaattt gcattttcga tttctccaaa ctgatcaatg tgggcgtaat tcgttccatc
      961 tgtcagtgta ggattagctg caccagctcg tgttggatca atgacaggct tgttttcaga
     1021 agttgttgga ttttcatcgg taaatccatg agctaaatca tatgctaatt tttctttact
     1081 tacgcccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaga tgttttgtgt
     1141 tacccataaa tgcgatttga ttcccggttg gttataagga gtgtccaacg ttaatggaat
     1201 accatatttc attgctgaat ctctagccaa ttcaacgtat gccttgtagt ttttctcaaa
     1261 cgttgcttta tcatgtgtgt gttgtaactc aatctgcaca ggactgttgg cattagcata
     1321 cgaaccagca ccgtactgta cataaccagg ttgaccgact tgataaacaa ttccgccgtc
     1381 tcccacaata taagcagtat aagcgctagt ccatgaacgt tgcatatact gcgcttcatt
     1441 gcgtcctgtt gctgtttcat tagccgtttc atgcagtaaa atatactgat tatttgctac
     1501 ttgtgagcta ccttcatttg cgcccaaatt aaattcattg ttgatagtat aggcaaatcc
     1561 gttaattggc aataaaaaaa gagccgttaa caggctcatc gcagtaatag taattttctt
     1621 tttcatttgt ttcctccttc ttcgctttca gccgagaaca ttttgtaggt tcgatttgat
     1681 acacccaaca cactccctaa aaacgcgcca aaaccagtaa tgatgacaac acagatatct
     1741 gtgtactgcc aattgagcgc tttaccaact aaccccacga aagtagctag tgcgggaata
     1801 attaccagtg cgaaccattt tagtacttcg aacgttttat tat
//
LOCUS       DAGCXG010000192         1838 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGCXG010000192 DAGCXG010000000
VERSION     DAGCXG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.199"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            76..1773
                     /locus_tag="MB022_002760"
     CDS             76..1773
                     /locus_tag="MB022_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467692.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          597 a          342 c          311 g          588 t
ORIGIN      
        1 atattatcat tgttattttt aacaggaatt gcttatttct tttattttat tttttctcct
       61 gcggacagca atcaaatgaa agaaataaaa gattcgtggg ttttctatac aaaagatgat
      121 cctgatttta agtttgacag caagtacact cgtacgattc ctaccgtgga taaagacgaa
      181 acattcatta tggaaacgac acttgaaaaa ccgttggacg aagcaaattt actaatcaaa
      241 ggaaaccatc aatggattac tgtatatcta ggcgaaaaga ttttatacca acggaaagat
      301 tatcaggaag aaagcaatcc cggactctca ttggctgtga tagatttacc agcaaattat
      361 atcggaaaaa aattgaccct tacagtctca tctccttatc agaactatgc tggactgcca
      421 ccgaaagttt atctaggaac aactggacca ttgattagtt ttatttattc acaatcagtg
      481 ccgcaagtat tgacgatgat aatcgctgtt tttatcgcta tagggacctt tttctatacg
      541 atctacttga tgtataaaca aaaacgtctt gaatactcac ttattctctt aagttgcttt
      601 gctttggcat taggatttga agctgtatca gaagatattt taagtggtat tctttttgaa
      661 ccattcgttc attctttttt atctcattta tttattatct taacgtcagc tttcatcatc
      721 tgctactatt ggagccgaat gttttactat aaaaaatggt acggaatttt ttgtacgttc
      781 caacttttga tttttatcgg cgttctgctt tatgcaactt ttactcctgc agaacttcct
      841 gaattaatgc cgattgctaa tattgctagt gttataagca ctttagtcac ttcacttgca
      901 tgcattggcg aagcatataa gaataaccgt ttttatgtag cttgtacacc ttggatcgtc
      961 ttggtagcca ttatccattg cttgatttac atccaaacgg cattaggtat ctatcaaaca
     1021 acgatcaatt ggtctacgat tttatatatt atcattctaa tcgttattat cagctacaat
     1081 ctaatcgatc acatcttaaa taccgacaaa gatcggagac aagtagattt cttaaaaatc
     1141 aaaaatgatt tgttggagca acattatgag cagcttcgac agcatatcca agaagtaaat
     1201 acacttcgcc tggaatttgt ccaagagatg gagaatctgc aagatttaat gcatacggac
     1261 caaacaaaag ctaaaaacta tgtggaaaag atattagaag aagcaaaaaa tttcgaaatg
     1321 ctgtgttctt tttgcaacca ccaggtgaca aatcttatct tagcgcgtta ccagaagtta
     1381 gctgccaaac ggaatattca gataaccttc caagcagatc ttcccgaaga acttcctatc
     1441 aacgatgacg atctggctca acttttgatc cacgctttag agcattcctt tagagaaact
     1501 catgccatcg aaaatccact gtatcgaaaa atttatttgt cgatacacga acaagaggaa
     1561 catttcgtca tttgttgcga acatagtgca cattacgata caaacctatt tagtcgaggc
     1621 atcacagaag aattagatga tcaggaacgt tttgacttaa tgatgatgaa agacgtagcg
     1681 gatcgttaca caggaacact aacgcaggaa aaagatactt tcattgatcg gatcactgtt
     1741 caactctctc gaaattaccc gaatcctgta taaaaaatag cggaatccat atatgtggat
     1801 tccgctattt tttattttca ataaagtaag gtaagcaa
//
LOCUS       DAGCXG010000193         1826 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGCXG010000193 DAGCXG010000000
VERSION     DAGCXG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.200"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(589..879)
                     /locus_tag="MB022_002761"
     CDS             complement(589..879)
                     /locus_tag="MB022_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467693.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1087..1778)
                     /locus_tag="MB022_002762"
                     /pseudo
     CDS             complement(1087..1778)
                     /locus_tag="MB022_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          486 a          354 c          286 g          700 t
ORIGIN      
        1 tacgatagtc aacaaaaaaa ttttcattat atccagaaaa atttttctta gttaaggacc
       61 tatggattgc agtaatgcag aattcgtcga ataaactgcc aaagacatac aatcatattg
      121 cttttaaaat tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa atgttaggtt
      181 taactgtcga accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt tacttttacg
      241 actatgataa tcacctcttc gagttacata ccggtactct ccaggaacgt ttaaaatcct
      301 acaattccac tacctgagac tagtaatgac tagtctcttt ttatggcctc tgatcccccc
      361 attgagcgaa tactaatagg gcttcgaatt gatctgttca atctttaagc aatatccctc
      421 aaatgagttt ggaaagttta gtgattcatc acaaatactt aaatacatgt agttatatat
      481 agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt ctgcatccat
      541 ctttccaaaa caagcggcta ggacattttt gtctcagccg cttgttttct agtttattct
      601 ttcatcttgt cggacctttt ttcttgggta tagacttttc tcatattcta ccacaatttt
      661 gggtttccct aacacatgat aggatctcac aacaaattgc tttctattat tataataacc
      721 tgtaacgact aacttcatcc cttcgtctac ttcagctagg aagttcaaac tatgggaagc
      781 aatcaaacaa ttgaccccat ttaaactgaa acggatcaac ggtctggcac taacttgcaa
      841 agtacggatc ttatctactg ttccatattt tttctccata accttcacct caataccatt
      901 atacgaacat acattccctt tgtaaagaag aaactctgtt ttatcttttt gactttttgt
      961 ctattacctt tcttaaaatc atattggcac ttcctgcgaa gatgaaggac atgtactaaa
     1021 aaaaagaggc agcagtatag attactgttg cttctttact aatttaggtt tacttgttaa
     1081 gaaagattat ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc
     1141 cacttcatct tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc
     1201 agttgcttcg tctaatttct tttgtgtatc tgttaactga tttctacatt tgttttttct
     1261 tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa ttctgaaagt
     1321 tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt gatttcatca
     1381 tttttttggt tgatctcatc tagtttgaca ttgatttcat tttgtttgct ttgtagttgt
     1441 gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga ttgatttttt
     1501 tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt tgaatcttgc
     1561 aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taatcgcttg ctctttatct
     1621 gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc tgtctctcca
     1681 ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag acatagaaat
     1741 gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc ttaatccaat
     1801 acttttacat gctaaaagta agtgat
//
LOCUS       DAGCXG010000194         1778 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGCXG010000194 DAGCXG010000000
VERSION     DAGCXG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.201"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            244..1722
                     /locus_tag="MB022_002763"
     CDS             244..1722
                     /locus_tag="MB022_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467694.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          647 a          245 c          312 g          574 t
ORIGIN      
        1 tttttgtggt tgtttacgca aatggcgcaa atcttgtaca ttttaacaca ataaaacaaa
       61 aaatatagaa tatttcctca taaatttttc cattttttgc aatattctca aaaaaaagat
      121 aaaaaatgag aataactgat gaaaaaacaa gcataatcca tcataaaatc agtataggtg
      181 aaaagatgaa ctttcatctg catatcaagt aattggtatg aggcattttt tgaggtgtag
      241 gatatgaagt tggattacct tatagaagag gaagatagag aaaaactgct gctagctaga
      301 tatttagaat ttgagaaaaa gaattatgta gtgctgccta agttatatga aacattaggt
      361 ttgtctaaat ttaaaacaag aaattatatc atagaattaa ataaagaatt tactgaattc
      421 aaaacaaagc cagagatcca aatttcagaa gaagcagaga tccatgtcga acgtctgaat
      481 ttgacggata tcaaaaattt aagaagaatc tactttcaac gatcaaaaat tgctcgactg
      541 ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta
      601 agcatatcaa gagcctatgt aaaaagaaga gaattagtct cttttcttga acagcatcaa
      661 ataaagttaa agaaaaacat attagttggg gaagaaataa acatacggaa cgtattattt
      721 ggggtatatt tcggggtctt taatggtttt tctatgccat ttagagaaag cgtcatgaat
      781 gaagcaaaga gtatcgtgca atattttgaa tatctgttca atttaaaact gtctgcaaca
      841 aaacgaatca aattcaattt gctaaccact attagtttgt tgagggccta tgggaatcaa
      901 ccaatcggaa aacgtttctt ttcagatgaa tactttaaga gctcagaagg aatgaagatc
      961 atcaaggatt tatcacatat ttcttttctt catatcccta aagagcagat cattgatgag
     1021 atttcttata tcatgttgtt tttgatcacg gaagaagaat ttgaattaga tggcgcattt
     1081 ggacttgcgg aagctcattt tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa
     1141 ttgattgatc aattaccatt ttcgattgaa atctctgaag aagaaagact atatatacga
     1201 aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa attggatatt cgactttgag
     1261 atgactagct ttacgtctaa aaagcagcta cagtttttct tcgagacgta tccaatattc
     1321 agtacagcaa tccaaaaggt catacaagag aataaacgct atttcttggg aaaagatgaa
     1381 cgtttcatga ttcgcttatt ttatgattat ctctttttat tggtcggtat ttgtccggta
     1441 tcatattttg aagcaccgat tcatgtctgt attgattttt cgcaaggatc tgcttatacc
     1501 gattacatta tttctcaagt gaaaggattt aaaaattaca atattgtgat tgagagtcga
     1561 atcacttctc gtacgaatat ttatttatct gattgtttac ttgaaaaatt taataaagaa
     1621 cagataattt ggaaaaatcc accgactcca gatgactggg aatattttgg aaataccatc
     1681 atcagagaaa aaaatagaca tttaactgaa aaaatcagct agtatttatg gatggcagct
     1741 ttgtctcaca tatttattag ctgatttatg tttttttc
//
LOCUS       DAGCXG010000195         1760 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGCXG010000195 DAGCXG010000000
VERSION     DAGCXG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.202"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..1059
                     /locus_tag="MB022_002764"
     CDS             <1..1059
                     /locus_tag="MB022_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HBT4467695.1"
                     /translation="KNVTGANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLF
                     TKQSGSIKDYKGTPRTVLVQRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENL
                     GPLGVRKMTLIDNDCFQAENLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLC
                     VEVESMNVLDLISMQPGFFKEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYG
                     IGGHTLFINYSLNQKGCFNCLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPY
                     NAIASTECAINAIKLFHKVNQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNK
                     FEERFFSKYCKVCGELDD"
     gene            1052..1513
                     /locus_tag="MB022_002765"
     CDS             1052..1513
                     /locus_tag="MB022_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HBT4467696.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1526..>1760
                     /locus_tag="MB022_002766"
     CDS             1526..>1760
                     /locus_tag="MB022_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467697.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVIS"
BASE COUNT          619 a          229 c          342 g          570 t
ORIGIN      
        1 aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag aaagaataga
       61 cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa tgtcataatt
      121 ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac gcctaggact
      181 gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg agaaaactat
      241 ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa aattgctgaa
      301 aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg ttttcaagct
      361 gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg atctcagaat
      421 aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaattccc atacctttgt
      481 gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg ttttttcaag
      541 gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc tttaaataat
      601 tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata tggcattggg
      661 ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa ttgtctttat
      721 acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa agaaggacaa
      781 atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa tgctattgcc
      841 tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca aaataatatg
      901 accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa tttaggatat
      961 caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga aagattcttt
     1021 tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg accgtattgt
     1081 cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg gaaaggatga
     1141 cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga gcataataat
     1201 taccgatata acaacacctc aaataggaga tatacaaaaa agattactct ttaaaaaaaa
     1261 ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata agttttctat
     1321 tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa atgttgataa
     1381 gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg tttttattat
     1441 cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata ttaggcaggc
     1501 tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt ggtgggggtt
     1561 tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga tgattacttt
     1621 tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg gagaccaaaa
     1681 agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt acattttttc
     1741 aattattatt gttatatcgt
//
LOCUS       DAGCXG010000196         1744 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGCXG010000196 DAGCXG010000000
VERSION     DAGCXG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.203"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            17..826
                     /locus_tag="MB022_002767"
     CDS             17..826
                     /locus_tag="MB022_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4467698.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            866..1363
                     /locus_tag="MB022_002768"
     CDS             866..1363
                     /locus_tag="MB022_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4467699.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          609 a          311 c          330 g          494 t
ORIGIN      
        1 aaagtaggaa gataaaatga aactagcagc gattgattta gacggtacct tgcttgatcg
       61 aaatggaaag ataccagaag aaaatatcca agcacttcgg aaatttgatg aaaatggcgg
      121 aatcgtaacc gtcgctaccg gaaggaacag catttctgct aaagatattt tttccgaatt
      181 aggaatcggt ggctatttga tttcttccaa tggttcactg attgctgaaa tgaaggatgg
      241 aaaaatggcg aaagttctca gacgttcaaa aatagatagc gatacactga gaaaagcctt
      301 cttctatgct caagaagcga aaatatccat cattgctagc cgagaaaccc aagatgatca
      361 gctgacattc gatgaatcaa atctagtcaa agatgaccct tattatgaac atttcaattt
      421 acagaaccat tcttttgatc aaattttaaa tatgttggaa gatccttctc ttagctatct
      481 taaattagca ttaacagata agagtgaaga aaaactacag aagctacaaa gagatctgca
      541 agcagatggt atccaatcag tattctctga tccgcatttc ttagaaatca cgcctacagg
      601 tatcactaaa gctcattcac ttgtttttct ggcggattac cttggaatcg aatcgagtga
      661 catcatggct tttggcgatc aagaaaacga cattgatatg ttgaattaca gcgggttgag
      721 tattgcgatg gggaatgcac agcgacaagt aaaagaaatt gcaaaccaag taacactgac
      781 gaatgaagaa gctggtgttg cttatatttt aaaaaacttc tattaaaaag aataaaatga
      841 cgatatgaat gtaggaggaa agaaaatgtt agttattatt gatatgcaaa atcatattct
      901 tgatccaaac agcgaatttt atattccaga gtcagatcaa ttggcagaac gtattgctca
      961 aagattagct aaagcacgcc aaaataatga atatattctc ttcacacggg atataccagt
     1021 tgaactgaag gacaaagaag aagaacgaga agatttgcaa ttgatccctc ttctttctcc
     1081 acaagaaaat gaacgagtga ttaaaaagta ttactttacg attccacctg aaactttagc
     1141 tgaaatccag gaaactttat ttgaaagcaa agaagaacaa aaagagatcg aagtagtcgg
     1201 gattgaaaca aacttgtgtg tactctctaa tctgataggc ctgcagagtg cttttcctga
     1261 agcagacttt tttgttgatc caatgttagt ctccagtaga aaacacggag aatccgcttt
     1321 agagctgttg aaagatttca acgtctctat cgtcgaatca taaaaatatc tatcttcatt
     1381 tacgatattt aacacacaat aaaaaaagca cgcagaactg ttttagtcct acgtgctttt
     1441 tctatcaaag tagattggct tttttatgac acagagtgaa acggcaggct cacgacctct
     1501 ttcttaaatg gtgttcaatc agctgaccct cggtattagc ttaacaaacg gcaaaatatg
     1561 aggaactatt tttccgtttg ttaagctaat actcaggtca aaacgctgat ttcacaacct
     1621 cttccttaaa cggtattcaa aagcttgcat ctgcagtaat aagaacagcc aagtaaaaac
     1681 ataagaagct gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1741 cctc
//
LOCUS       DAGCXG010000197         1716 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.204, whole genome shotgun sequence.
ACCESSION   DAGCXG010000197 DAGCXG010000000
VERSION     DAGCXG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.204"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            658..897
                     /locus_tag="MB022_002769"
     CDS             658..897
                     /locus_tag="MB022_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467700.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1259..1519)
                     /locus_tag="MB022_002770"
     CDS             complement(1259..1519)
                     /locus_tag="MB022_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467701.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          588 a          297 c          229 g          602 t
ORIGIN      
        1 cacattcgct atacatttgt aagaagtaat aattcctgta ctaaaaggca taattgcttt
       61 aattattttt tgacttctct tcaaacacaa gaagtagttg tgtaataatt gcagaaataa
      121 ggattccaat aatagatata aaatttgaca tggaaacgtc tataacatta aaagaataaa
      181 gtgttgaaga aagtaaactg attgaaataa cccctccgta aaaaatagtc tgtgaatata
      241 cccacttttt tcagaagacg tacttcgttt ggttctaaaa cctatccctg catttctact
      301 cttatcaaaa aaccaaatat ttagaagtaa taatattgct attattaaga taaaattact
      361 gtaaaaatcc aaaagctatc ctcctagcaa ctatttaaat acccaaaaaa attacctcta
      421 gataatatca tacctacgtt ttcacttata aattttttca taaaaaaagc gtttagaggc
      481 ataatcgtta atacagatgg taaccaatgt atctggtttg aactgggata tcaccctctc
      541 catatatgta ttactaagac taatagatac tataaaagac tggatagttt tgcattgatt
      601 ttgcctaata ctgagcatag gtgtatgcta aacgatacta taaggagtgt gtacataatg
      661 aaaagaattc tgtttatcag tttatcaaat ttattgttac ttactgcttg tgggacttct
      721 gcatcaaaga acataccttc agatacgcaa atttcgaata gtatcgattc aacaatttct
      781 acaaatgaga aaaattctcc ggtagaaact tccgatataa aagtttctac ctctgcaaaa
      841 aacagggtaa agaaactgtc agcacgtctc ctttatttca aacaaactct gaattagctt
      901 cttcatcttc ttcagcaaat aatgatgata gtttcgcgtt tactcctaac taaatgcaac
      961 ctttacaaat gctttacgat aacaagcctg agctaaatga tcctgatatc agttttgcat
     1021 tttttgaaat gattggatcg gatgatcttt ttaaaactac tagtttgtct atccgaaaat
     1081 aggacagttc tggcagtata ggtttttatc gagtttctcc accaggcctc gttacagaga
     1141 cagattctct aggcgaacct tttgaataaa tcaaaaatca tgaacaatca ctgccaacta
     1201 aataaagtgc cttcaaaagc ttggtcttct cgcttttgaa ggcactttat ttaaatcttt
     1261 atgaaactaa agtatgttca tatttgttgc tatagttata tagatattct gttgtttcca
     1321 actcataaac gtgagtatat tttgccacta cttttccgca tttttctaat atttcattgc
     1381 gatcgacgtc atgatattcg tctacgcaga tcacaacaca attttctaat ttactgatta
     1441 cttctccaac cactttttgt ttcaagccaa taggttggca aatatatttt tttcctaaaa
     1501 tgatagtttc gttattcata tttcatccat ccttaatgtt ttttcattat ttaaaatgat
     1561 aataccatta aaatatgatt atgaaaatca ataacacttg tgattttata aaaaaataaa
     1621 aaaaccgaaa aacagaaatg cccactgtct ttccggctct ttttattctt gatttctctt
     1681 cttttttgtt tcaatagacg attttagtta ttaata
//
LOCUS       DAGCXG010000198         1698 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGCXG010000198 DAGCXG010000000
VERSION     DAGCXG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.205"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..903
                     /locus_tag="MB022_002771"
     CDS             <1..903
                     /locus_tag="MB022_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBT4467702.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1698
                     /locus_tag="MB022_002772"
     CDS             916..>1698
                     /locus_tag="MB022_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBT4467703.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFS"
BASE COUNT          564 a          248 c          389 g          497 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttca
//
LOCUS       DAGCXG010000199         1691 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGCXG010000199 DAGCXG010000000
VERSION     DAGCXG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.206"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            194..1486
                     /locus_tag="MB022_002773"
     CDS             194..1486
                     /locus_tag="MB022_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4467704.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          535 a          290 c          346 g          520 t
ORIGIN      
        1 gccctcattg gaacactcaa tatattatca gatttaaatt tcaaatagat aatttcattg
       61 tttagtaata tcgaaagata cttttttaat gatgagagtg taatataaac cgtgtaagta
      121 accattaatc ctaggcaggt ggttgcttcg cggtttttct ttttctacaa taagatcaag
      181 aggtgattac acgatggcta gaaagaaaag aaatcctgat gccgaaaagt tagctgaatc
      241 cattctgaat gcctatcaac ctgaatctgt cgatgacatg caagatgctt tgaaagatgt
      301 gtttgggccc ctttttgaaa aaatgcttca aggagaattg aataatcatt taggttatga
      361 tgcccattct aaagagccta aggaacacga taaccgtcga aatggctatg gaactaaaac
      421 gcttaaaacc agttttggtg aagtagctat tgatgttcct agagaccggg aagcttcctt
      481 tgaaccagag ttaattccta agagaaaacg agatgtctcc gacatcgaag ggaaggttct
      541 ttccatgtat gcacgaggaa tgagtcaacg agatattgcc gcaaccgtcg aagctattta
      601 tggctttgat atttcccatg aaatgatttc agatatcact gacgctgtcc ttcctgaatt
      661 ggaagaatgg caagcccgcc ccctagcaaa gtgttatgcc tttctattcg ttgattgtat
      721 gtatgttact ttacgagaaa attatgaagc caaagaatat gctgtataca ccattcttgg
      781 ctatgatctc aaaggaaata aggagatttt aggattatgg ttaaatcaaa cagaatctaa
      841 aaatcgctgg atgcaagtat ttgatgaact gaaagcacgt ggtgtcgaag atgtcttttt
      901 catttcgatg gacggtgttt ctggcctcga agaaggcgca aaagcgatct ttccatcggt
      961 aattgttcag cgctgtattg ttcatcttgt tcgaaatgca ttgcgctata ttccaagtaa
     1021 ggactataag gaagtctgcc gagatatgaa aaagttctac ggtgcttctt ctctaaacgc
     1081 tgcacatgct gcttttgaca gttttcaaaa tcggtggtct cattattctg gtgctgtaga
     1141 tgtctggaaa cggaactttg cacatgttga acaactattt gattatggta gtgccattcg
     1201 aaaaattatg tacactacca atgcggttga aagtgtccac tccagcttca gaaaagtcac
     1261 aaaaaaagga gcgttctcta atgagaatgc actcctaaaa ctactttatt tacgcacgaa
     1321 agaattacac gcgaagtggt caggtggtcg aattcaaaac tgggctatgg tgcttaatca
     1381 gttaatgatc aacgagacct tctcctctag aatgaaaagt atgcgattta ccttccgtaa
     1441 caacggtagg tttattcaac tatgtgtccg atcaaaattt atctgatttc aaaatagccg
     1501 agagaattat ggtggttaaa aactcttaac tatttaatta cggttttcat agtttttgga
     1561 ttcaggagga ttaaaacgaa ttctataaaa aaagatttgg tttaaatgaa tgattaaacg
     1621 acaatttagc aacgtagtca gagtaaaaca gctctttctt ttcgtatttt ttaaagttgt
     1681 tacgaaaaag t
//
LOCUS       DAGCXG010000200         1688 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.207, whole genome shotgun sequence.
ACCESSION   DAGCXG010000200 DAGCXG010000000
VERSION     DAGCXG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.207"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            153..749
                     /locus_tag="MB022_002774"
     CDS             153..749
                     /locus_tag="MB022_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4467705.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(807..1289)
                     /locus_tag="MB022_002775"
     CDS             complement(807..1289)
                     /locus_tag="MB022_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467706.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1395..1592)
                     /locus_tag="MB022_002776"
     CDS             complement(1395..1592)
                     /locus_tag="MB022_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467707.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          561 a          343 c          260 g          524 t
ORIGIN      
        1 cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt ttttgtgagc
       61 atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca attaaaaaaa
      121 tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac actttgaaaa
      181 accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa gaattaacaa
      241 ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa tcaacacgcg
      301 cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa gaagcacttc
      361 gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct ggtttcaaaa
      421 atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt ctacaagaac
      481 aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac ggaatcgcaa
      541 cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg caacattaca
      601 acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac tggaaactac
      661 gttcgcaatt agtattcggt tcaccagaaa caccggctgg ggaaaaagaa tacatgaacg
      721 atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca gaggttgtga
      781 caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc aatctctgcc
      841 tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc agaaaatttc
      901 agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt agaacctttt
      961 ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt gtcaaagcta
     1021 ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact aataaaaatc
     1081 attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat gagtgtatac
     1141 caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt tcgcacagtc
     1201 cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac aacgaataaa
     1261 aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc ttctccagaa
     1321 aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc acgccgcacg
     1381 tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct tgctttttca
     1441 tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc gcagtttgtc
     1501 atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt gcatgcatat
     1561 tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg ctggaacttt
     1621 tctccacatt accactgcta tttctttgga gatttaactc attgattttt atttacagtt
     1681 aaaaatca
//
LOCUS       DAGCXG010000201         1665 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGCXG010000201 DAGCXG010000000
VERSION     DAGCXG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.208"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            132..506
                     /locus_tag="MB022_002777"
     CDS             132..506
                     /locus_tag="MB022_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4467708.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="MB022_002778"
     CDS             complement(620..1249)
                     /locus_tag="MB022_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4467709.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="MB022_002779"
     tRNA            complement(1383..1455)
                     /locus_tag="MB022_002779"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          558 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tactt
//
LOCUS       DAGCXG010000202         1659 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGCXG010000202 DAGCXG010000000
VERSION     DAGCXG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.209"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..233
                     /locus_tag="MB022_002780"
     CDS             <1..233
                     /locus_tag="MB022_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467710.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            392..>1659
                     /locus_tag="MB022_002781"
     CDS             392..>1659
                     /locus_tag="MB022_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4467711.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIR"
BASE COUNT          536 a          322 c          304 g          497 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
      361 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      421 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      481 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      541 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      601 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      661 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      721 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      781 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      841 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      901 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      961 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
     1021 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
     1081 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
     1141 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
     1201 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
     1261 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1321 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1381 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1441 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1501 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1561 cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg cgtacggctt
     1621 tcgcaacttt tacaatttca aactacgaat tatgattcg
//
LOCUS       DAGCXG010000203         1623 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGCXG010000203 DAGCXG010000000
VERSION     DAGCXG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.210"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..173
                     /locus_tag="MB022_002782"
     CDS             <1..173
                     /locus_tag="MB022_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4467712.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
     gene            complement(378..668)
                     /locus_tag="MB022_002783"
     CDS             complement(378..668)
                     /locus_tag="MB022_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBT4467713.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(804..983)
                     /locus_tag="MB022_002784"
     CDS             complement(804..983)
                     /locus_tag="MB022_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467714.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(980..1330)
                     /locus_tag="MB022_002785"
     CDS             complement(980..1330)
                     /locus_tag="MB022_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467715.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1323..>1623)
                     /locus_tag="MB022_002786"
     CDS             complement(1323..>1623)
                     /locus_tag="MB022_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HBT4467716.1"
                     /translation="LQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQK
                     QINEENLDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          524 a          304 c          234 g          561 t
ORIGIN      
        1 tcaataagca aattaaaaga tacagccgta gaaaagagca gtttcaaaat gaagaatcac
       61 tagaacgctt tctagtcagc atttttgata catacaatca aaaatttcta aacagaagcc
      121 ataaaggttt tcaacaggta accgatacat tagtttcaat gtttactgag taactaatta
      181 ttttgcagga ggacaattta tttacacaaa attattgacg ctccctttgc gagcagcaac
      241 tagaataatt taaacattat taaactgtct atattgttac taataattcc cttcttatag
      301 tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta
      361 tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag
      421 gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta
      481 gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg attttattct acgttgtcgg
      541 gttatccagc ttgcaaccgt tccttgtggg taggcatata actcacaaaa ttgttctaca
      601 gtgattccta aattcttgat aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg
      661 atagacatgg tgcgtaagcc tactttcttt tattattgcg tttgcaataa tagtttaaca
      721 cacgcattat tctagtatct actcctattt gtaaaaacaa aaaaggattc atagagataa
      781 ctctatagaa tccgtaaaat accttatata tataacggga aaatataaaa cctaaaatta
      841 tgataacctg ctgcagcttc tacatagaaa gagaagcgct caacaccaac ctcatttatc
      901 aaacgttggc cataacccca aatctcttct gatagctgat caaaggttat atactttagc
      961 tcatattgct ggataatgtt cattctaatc gactccattt attatcctgg taaaattcga
     1021 tatgacgtat ttcgtcgtag tgaattttgt cttttccaac gtagatcccc agctcatcac
     1081 tacctaagat cttgccaaca atatcttctg gatagtatcc ttcagcgttt cgttcttctt
     1141 tttgaataga tactgatcta ttttttatga ctgcagcgtt caatacttcc gcaatctctt
     1201 cagtcgtcat ttgttctttt gctaaatttt gactggttcg ttgttcattg tcggcatcaa
     1261 tttcttcagt atgatcagat aaataaaacc ctgcccactt taaacgtcca cgatcattgt
     1321 aatcaatcat ttttaattcc cccatttccg cccgcatgac caccaacaag tgtgcttcta
     1381 gtaatgcttc tagcgctttc taacattgaa gaagcatgta caatcgacgt aaaaccatat
     1441 ttttgccgaa tcttgtcaat aattttatct aaattttctt cgttaatttg cttttgtgga
     1501 tcactaaata aatccagctg aagcgaatcg gtgtacacaa gcttgctata ggttattcca
     1561 acgtgcctta cttcttgtcc ttcataatac ttatcaaaca agaaaagaca ataattctgc
     1621 aat
//
LOCUS       DAGCXG010000204         1608 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.211, whole genome shotgun sequence.
ACCESSION   DAGCXG010000204 DAGCXG010000000
VERSION     DAGCXG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.211"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(190..1005)
                     /gene="thiD"
                     /locus_tag="MB022_002787"
     CDS             complement(190..1005)
                     /gene="thiD"
                     /locus_tag="MB022_002787"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBT4467717.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1299..1574
                     /locus_tag="MB022_002788"
     CDS             1299..1574
                     /locus_tag="MB022_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467718.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          490 a          319 c          251 g          548 t
ORIGIN      
        1 aagctgtttt ttgaacttgt tccttgtgac tcggagctgg acggcaggct cacgacctct
       61 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca
      121 taagaagctg tttttacttg gctgttctta ttacttgatg cagggcaact ttttcacaac
      181 ttctttcttt tactctaatt caccagctgc ccaatgattg atggggccat atttgtgccc
      241 tacatttatt tcttcacgaa ttgcacggtt tacatagtct tttgccaatc ggatcgcttg
      301 ttcaactgtt tttcctttcg ctaactcagc tgcaatacat gcagatagcg aatcgccagt
      361 tccgttgatc cggtcggtag gataaaatgg tgcatttaac caaaatgatt ttcctgattc
      421 gaacaatacg tagtcattga ctgtttcttg ctgcgcattt ttagcatgat gtcctttgat
      481 cataacgttt tttgcaccca tatcttgcag ttcttttgct gcttgcacaa aatcttcttc
      541 agctgctaat gtccgtccag tcagcttttc tgcttcataa aaattaggtg tcagtacttc
      601 ggccaaaggg acaagctttt ctttcaagct ttgcaaagca gattcttcta gtaaaagatt
      661 tccatgtttc gttacgatta cgggatccac gactaatggt ccaaaatcat atttttggta
      721 attttttaca actgtctcaa tcaactttga atctgctagc atacctgttt tagctgcacg
      781 tatttgaaag tccttcgcaa tcagttcaaa ttctttgtcg ataaaatctg ttggcaatgt
      841 cacacttgct ccgattccgt aagaattacc tgcaacacat gcggtcatga cagaaacacc
      901 gtaaacattt cttgtaaaga atgtatgcat atctgcttgc atccctgcgc ttccatcgct
      961 atctgatcct gcgatcgtca ttgcttgtac atactgatct tccatcaaat cgattcctcc
     1021 atttttataa aaagattttt cataatactg ttactgtttc tatcatatgt ttttcaaagt
     1081 atccctcagt aaatatacca ctaccttttc ttggtaatca aatttatttg aaaaaacatt
     1141 tgcaataaat cagaaaatag tggtcgttta tttgtattcg ctattctaat aataaataac
     1201 cactatttga tttatatata tttcagtatc cttcttgttt tcatataacc aaaggcgcta
     1261 tgatttccat atgaaaaaaa taaagtcagg tgaaaaaaat gaaacttgag gataaaaaag
     1321 tcattttgaa gaaaatcaaa cgactaccag accaaataga tggctatacg atctgttctt
     1381 tcaaaaaaga caggagtata aaagcttata cgcaaaacac agaaaaagga ataatctatt
     1441 tcctacatga agaaggttac gatacccaaa cctttatgtt tactgaaaaa aaagaatttc
     1501 ataaacagat gaaaaaactg attgaaaaag aatttccaag aagtcataaa ctctatgtga
     1561 atcagcaatt ttaacttcta actgttcttt attaattaaa aatgttct
//
LOCUS       DAGCXG010000205         1606 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.212, whole genome shotgun sequence.
ACCESSION   DAGCXG010000205 DAGCXG010000000
VERSION     DAGCXG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.212"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(908..1426)
                     /locus_tag="MB022_002789"
     CDS             complement(908..1426)
                     /locus_tag="MB022_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBT4467719.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          498 a          312 c          223 g          573 t
ORIGIN      
        1 gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc tacaacttat
       61 aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag taaagtttgt
      121 tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc tgaaaactta
      181 tgaaatgaaa gaccttttac atcttgcaga aatcggtact gggattaaac taagcaataa
      241 aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat tctgataaat
      301 gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga tctcttcagt
      361 atcagtaata ggataggcga taggccacat accctttgat ggttagaata aactgctcaa
      421 aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa ttataaacca
      481 taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca gaagatataa
      541 ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta aagggaattg
      601 tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc ccctgctagt
      661 ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga agtgagtcat
      721 ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc tacggcattc
      781 taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga acttatgtag
      841 tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct tagcagtaaa
      901 cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa gtcccaaatg
      961 gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt ttggttgcct
     1021 cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt gaatcaatca
     1081 tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc aatggtagat
     1141 gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat ttaaattgat
     1201 cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt agaattaaat
     1261 tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact tcctcaactc
     1321 ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta gtataagtcc
     1381 tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata ttacctccat
     1441 aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt ttaatccgtt
     1501 ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga tttataaaat
     1561 atcccaacgt ctcctaattt cagcgttcgt catacggcaa agagcc
//
LOCUS       DAGCXG010000206         1570 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGCXG010000206 DAGCXG010000000
VERSION     DAGCXG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.213"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..790
                     /locus_tag="MB022_002790"
     CDS             <1..790
                     /locus_tag="MB022_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4467720.1"
                     /translation="QVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKV
                     SADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKII
                     ENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLP
                     FDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIAT
                     HDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
     gene            808..1377
                     /locus_tag="MB022_002791"
     CDS             808..1377
                     /locus_tag="MB022_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBT4467721.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          560 a          191 c          299 g          520 t
ORIGIN      
        1 tcaagttcta tttgttaaaa atagaactgt aaaaactaca actattcagt tgttaaaaac
       61 tgaaaacaat aacgcattaa ggaaatttga catttttaat agttcgattg aagttcccta
      121 tatcgaaaag gtaagtgcag atgtattaaa agttgaagaa attacttatg aaaatattct
      181 aaaagacata aattttgatg tcaaagttgg agatgttatt ggtttggtag ggaaaaacgg
      241 tgtggggaaa acaaccttac taagaatact catgaagatt attcgcccta ccaaaggaaa
      301 gataatcgaa aataaaaaaa atttgtcatc gccgttttta gtaatgcaag aaatggatta
      361 ccaatttttc acagaatcag tgaggagcga gttagccctg gggaatgaaa tagttagtaa
      421 agatcagcaa gaaaaaattt taaaaaaaat ggaactttat aaaatgaaag atcaattacc
      481 gtttgattta tccggtggag aaaaacaaag attattggtt tctatagctt cattgtcaaa
      541 aactaattta tttctatttg atgaacctac aagtgggttg gattatatta atatggaaag
      601 gattgctgac cttattaaag acttaaaaga aaagggtgca gtagttatag ccactcatga
      661 tccggaattt ctttataaaa catgcaatag aattatttat ttaaaaaatg gtggaataaa
      721 aaaagatata aaacttagag tagaagattc aactttgata gaacagattt ttaatgacat
      781 aacgaaatag aattgaggag aatatatatg aaaacaaaag attatatttt tattggttta
      841 gctacagtta tttccctagt aatttacttt ttttcaataa tggttagtag tattggtggg
      901 gcgtttggtc acagcatatc cccagggatt tttggattat tatctggaat catatttgtt
      961 tatattagct ataactaccc tagaaaagga atttttacta tttacacaat tgttttattg
     1021 ttatttttta cgttaatggg cggggcgtac ttaccttggt ttatcagttc gattagtatg
     1081 gcgatcttag ctgatattat tctatctgta tttggatatg atcgagcaat tcctcaagtt
     1141 gcgtcttggg ctctaatgca attaggcagt gctgccggac agtggattcc tatatggttc
     1201 tttacagatc gttttcgtca agattggata gataaaggac aatctgctgc aacaatggat
     1261 gcaatgatcc actatgcggt aggtatatgg ggaatcattt cagtgttagt tgtagcaagt
     1321 ttatctatga ttggtgtatt gattggaaga aaagtcttaa aaaaatacaa aaaatagaac
     1381 gtagtttacc ttctatctgt ttgtacatct ctaatttacg ctattacgga aagcattaac
     1441 agttgtaaac gaataagaga agaaattgaa tgcgggggag gaaaaaatga aagtatacaa
     1501 aaaattattt agttatgtcc ctaaaatgaa atactacgga tggttagcca ctttattttc
     1561 tgatttttct
//
LOCUS       DAGCXG010000207         1555 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGCXG010000207 DAGCXG010000000
VERSION     DAGCXG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.214"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..243
                     /locus_tag="MB022_002792"
     CDS             <1..243
                     /locus_tag="MB022_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467722.1"
                     /translation="RTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRLVYKSI
                     VEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(408..1232)
                     /locus_tag="MB022_002793"
     CDS             complement(408..1232)
                     /locus_tag="MB022_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467723.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(1256..1411)
                     /locus_tag="MB022_002794"
     CDS             complement(1256..1411)
                     /locus_tag="MB022_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467724.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT          520 a          260 c          197 g          578 t
ORIGIN      
        1 cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga tcgcaaaagc aaacggcgtc
       61 tacaaagggc ggcccaaatt atacagtgca gacgcaaaag atccccaacg tcgccttgtt
      121 tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga ttgctaagat tgctaaagac
      181 tacaatgtta cgagacaaac cgtgtataga ataaaaaaag acagcatggt caatgacaaa
      241 tagttgatca tgctgctttt attatcttct catctttaat ttcctacata taattactat
      301 ctataaaatt aagtcttact cagaacagat aaagcataaa attccaacaa accatttttt
      361 acttaactaa ataaagagtc tgttaatata atcttcactt atttacgtta atcccctact
      421 gtatttgttg gttccttatc tatattttct acaaaatcct tattatcttg tgatttagct
      481 tcgtataatt ctttgtatgt cgacacattg gttatactaa ttcctatagc tagttcttga
      541 gactctactt ttctctctag ctcctttgac aaagtgattt gagatttatt tggttccgtt
      601 ggagtcaatt ctatagattc ttcataaaaa tctctataca taaagctatc attaatttta
      661 taaaaaaaat taaatgtttt tagcactgcc atataacatt caaaacattc ttctatagtt
      721 ggtttacttc cttgaggaga atgaaccaat ttattacgtt tttctatgaa atttttaatt
      781 tcctttctta tttctttcgg aaactcaata ttaaagtacc ctttaaacat gctccctaat
      841 aatttgtcta cagggggaga ttgtaaattc tcaaatatta gagttaaatc aggtttctct
      901 tgttgataaa attcctttac ccctatttca caggctgtaa ttatcgaaat aaacctatct
      961 ctagtatttg atatattttt acttctttta aggagtataa gagaaaaggg gatactttcg
     1021 tgtccatgca tgtaattaac taattgttga gcttcagaag ttatatctct aattatatag
     1081 ttaacggaat taggaagtgt ctttaactct aatgaaattt ctatccaatt actatcttct
     1141 aatctgtata tgctcgaagt ataatttgat tcgtcagcaa gcatcgctac atcccaaaca
     1201 ctgatcataa aatcttttaa acgttcaatc atttcagcaa tattgctaaa atcagctaat
     1261 ttttctttag aataaaaaaa acatttatat cagaatcaat aaattctatt ttttttatta
     1321 ctatatcgtc atcattatct tgaccattta tgtttattgt tccctgagtg gggagtactt
     1381 ctttcaaatt caagttaaat ttatattcca ttacttctcc tccaatatat aaaaattact
     1441 taactgtatc caaatttaat atgtaaataa atcattaatt tttagctaaa ttctatgaga
     1501 agtttatttt tgttgttttt gctaattaac taaaactagt acaaaaaatt taaaa
//
LOCUS       DAGCXG010000208         1555 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGCXG010000208 DAGCXG010000000
VERSION     DAGCXG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.215"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            132..644
                     /locus_tag="MB022_002795"
     CDS             132..644
                     /locus_tag="MB022_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467725.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            915..1538
                     /locus_tag="MB022_002796"
     CDS             915..1538
                     /locus_tag="MB022_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467726.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          611 a          191 c          263 g          490 t
ORIGIN      
        1 gccataaacc gatgagaggt acttatataa aatttctcga aaattataat gatttaaaat
       61 ctaccaataa ttatcaattt ggaaacttgt cttatgagat gataagtaaa gttctaagtt
      121 tgtgatgata aatgaaatta aatagtataa atgatttatt tgctattata ggtgttattg
      181 ctaacatact tttttcgtgt ttgaatttta gatttactca aaagaacaaa aagaacaaac
      241 tcagtataga acaaatgaaa aaagacattg aaactgattt aaaaaatgac cataaagaaa
      301 gaagaaaaat tgaagaatat acttctttaa tggaaaagtg taaagcaact gatgcaattt
      361 ttagcttttt aagaaaaagt gatgagataa tcacacgtga aaatatagat ttagaaaatg
      421 agattagtaa acttgaatct atcttttccg atataaattc tcttaagtat ttaatcgtcg
      481 aagaaaattc ggatagcttt ttagaatttg aaaaatatct aacagatctt atagataata
      541 gaaataatga attaatgaga caaaacattg caagattcct tactaaatta gtttctgaat
      601 ggaaaagtat tatttctcaa aatatggata aattaagata ttaaggatgt tagttttaaa
      661 ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat acttaaagga
      721 ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta tttgttgttt
      781 cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga tttttcagcg
      841 gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa tagattggat
      901 aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat caaagtttag
      961 ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct gaagacgatt
     1021 taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca gagctgcaag
     1081 agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt gatcgattag
     1141 caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa ggtgcttcta
     1201 ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta agagacttgc
     1261 taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg aaaaaaatca
     1321 aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat actggtaaaa
     1381 agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat gaaattgttg
     1441 atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga gttacaagaa
     1501 aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt agaaa
//
LOCUS       DAGCXG010000209         1518 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGCXG010000209 DAGCXG010000000
VERSION     DAGCXG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.216"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            5..1471
                     /locus_tag="MB022_002797"
     CDS             5..1471
                     /locus_tag="MB022_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBT4467727.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          415 a          289 c          321 g          493 t
ORIGIN      
        1 gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat tgtcaacttt
       61 gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat tacttatcta
      121 ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa cagcattagc
      181 tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg gatgggtatc
      241 tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca ttatgcttgg
      301 ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga tggcattgat
      361 tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac atctttattc
      421 aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg tcaacttagg
      481 ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact atcatttagg
      541 gttctcttta gctgctattg ggatgttctt tggattatta caatacatca tccaaggacg
      601 caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg aagaacgtaa
      661 aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat tcggcggggc
      721 acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg tattggggat
      781 tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa ctgctgaaga
      841 aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt tctggtcatt
      901 acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac aatctacgct
      961 atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt tcatctttat
     1021 tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac catctacagt
     1081 agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat tgatgttgcc
     1141 gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg gaagtttctt
     1201 catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga caacaaaatt
     1261 agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg atgcttcttc
     1321 acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat ctgcttactt
     1381 cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg tgaaagaacc
     1441 aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag gaattgctta
     1501 tttgcagcag ttcctgtt
//
LOCUS       DAGCXG010000210         1502 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.218, whole genome shotgun sequence.
ACCESSION   DAGCXG010000210 DAGCXG010000000
VERSION     DAGCXG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.218"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..848
                     /locus_tag="MB022_002798"
     CDS             <1..848
                     /locus_tag="MB022_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4467728.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1389..>1502
                     /locus_tag="MB022_002799"
     CDS             1389..>1502
                     /locus_tag="MB022_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBT4467729.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          496 a          266 c          291 g          449 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaatttta
      961 tttatggtat gacataaata aagtaaagtt attcttctag gttaatctta ttaatactct
     1021 ttctcactag tgttgcatgc aacactagtg agaaagagta ttaaatatac tgcaaggcaa
     1081 gacatttggt aattgaagaa taacaagcaa aaaaatagca gtcaggtctc ttgacaaagt
     1141 atagttgcta ttcttttttt aggccctact ttaatgaaat ttttgtttgg agggaacgac
     1201 ttcattcctt tgattatttc agttgaattt aatagagaag cctggcgtct attaagaaat
     1261 acttttcttg atgagcgtca atttttttaa cggaaatcta tcctatacta aattcggttc
     1321 tgttgcaaag ttttccaaaa aatctatttt agtgtaaaat tgagaaaaaa gacagagagg
     1381 acagagtaat gaatcatttt aaaggcaaac aattcaaaaa agacgtcatt attgtcgctg
     1441 ttggttacta cctgcgttac aatctaagct atcgtgaagt tcaggaattg ttatatgatc
     1501 gt
//
LOCUS       DAGCXG010000211         1498 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGCXG010000211 DAGCXG010000000
VERSION     DAGCXG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.219"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            5..403
                     /locus_tag="MB022_002800"
     CDS             5..403
                     /locus_tag="MB022_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4467730.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="MB022_002801"
     CDS             387..1082
                     /locus_tag="MB022_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBT4467731.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          566 a          215 c          303 g          414 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttt
//
LOCUS       DAGCXG010000212         1481 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.220, whole genome shotgun sequence.
ACCESSION   DAGCXG010000212 DAGCXG010000000
VERSION     DAGCXG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.220"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            353..1393
                     /locus_tag="MB022_002802"
     CDS             353..1393
                     /locus_tag="MB022_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4467732.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          581 a          195 c          234 g          471 t
ORIGIN      
        1 ctactgaaat aaggtagtat atattactag taatggtagt atgttttata tgcataaggt
       61 agttcttaat ttattttttg ttttgacact ggtagtactt aattatcttt aattttaaaa
      121 ctaaatttta ttcatacgtt tttctggtag tatttaatta taaataatat ttatttaaag
      181 tagttcttaa tgactttaaa ggtagtacat aattcatttt atttctgatt ttaggtagta
      241 tttaattaaa aaacagtttt aatgcgtttg cattttttat ttatctttgc tattttcgaa
      301 aagagtaggt aattttacat aaataaaaca acgtgaaagt agaggatcta caatgactga
      361 ttttaaattt tttaaggcgg atagagttta caacgaatta ttttatcaat ttccaaaagt
      421 ctttattgtt tctgacgaat acaaaaaaat gaaagattca actaagattg cctatatgct
      481 tttaaaagca agattagaga tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt
      541 ttattttact tatacgacaa atgaactatg tagagtatta aactgccaaa aacaaaaagc
      601 gatagcaatc aaaaaagagt tggaatcctt tggtttatta ttacaaaagc agatgggatt
      661 taacaaacag ttagggaaaa ataatcctaa tagactatat ctagcagaat taaaagtctc
      721 agaaaatgat atctacttac tcgaaaaatt tgatagagag aatagggaaa acgttgataa
      781 atcagagggt atgaaaatca tacccaccct cgacgaaaaa tcagacgctg aatcccttgg
      841 ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag agggtatgaa
      901 aatcatacca gaacttaata ataatatatt agacactaat agacacaata tagacactga
      961 aaaagaccgc ctacaagatc aattgttgtt agacaatttt gagacaatta tgacaaacga
     1021 cagcattgct acgtttgtcc ctgaacgagt attaaatttg ataaaaacat tttcttcaag
     1081 ttacagtgaa gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa aagctgaaat
     1141 agaaagtggt atttcgatag tttttgaaga actcgatagt tattatgtca atgcagaaca
     1201 agaattatac acgacactgt taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa
     1261 tatccagaac ctgatttttg tctatgtaaa aaattggttt atcgaaaaac caatagctgc
     1321 taaagtatca agtgaaaaac gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga
     1381 ctggttagag tgaaacttta ctgacaaagt gattgttgct tcatggagta acattaaaat
     1441 taaaaatttt ttattattct aattatatta cgaatattac a
//
LOCUS       DAGCXG010000213         1469 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.221, whole genome shotgun sequence.
ACCESSION   DAGCXG010000213 DAGCXG010000000
VERSION     DAGCXG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.221"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            10..966
                     /locus_tag="MB022_002803"
     CDS             10..966
                     /locus_tag="MB022_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HBT4467733.1"
                     /translation="MTRSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMS
                     IRDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYT
                     RRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLDIGTSILETRPGFSHQMKIPITDNTK
                     ELQNYCLFLFDKYYEGQEVRHVGITYSKLFYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            959..1309
                     /locus_tag="MB022_002804"
     CDS             959..1309
                     /locus_tag="MB022_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467734.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTNHNLAKEQMTTE
                     DIVKVLNAAVIKNRSVCIQKEERNAEGYYPYDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT          524 a          242 c          319 g          384 t
ORIGIN      
        1 atcttaaaag tgacccgatc actgaatctt tttacgactg aaggaacaag aagtcagcga
       61 aggaagaagc tcgctcaaat gatccaagaa cgtattaaag aagagttagg tttgattgct
      121 acggtaggcg tcggcgataa tccccttttg gctaaactag ccttagacaa tgaagcaaag
      181 cataatgaag gatttattgc ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat
      241 attcctgaaa tgacggactt ttggggcatt ggctctcgaa tgaagaaaag actcaatcag
      301 atggggatta tgagcattag agatcttgct aactggaatc catacaccat taagagccgt
      361 ttaggtgtga ttggcttaca gctatacttt catgcaaatg gtattgatcg aactgatata
      421 gcaatcccac cagaaccgac aaaagaaaag tcctatggta attcacaagt gttgccacgt
      481 gattatacta gaagaaatga aatcgagctg gttgtaaaag aaatggcgga acaagtagcc
      541 attcgtatca gacagcataa ttgtaaaaca ggatgtgtcc atttagacat tggtacatcg
      601 atcttagaaa cgagaccagg gttctcacac cagatgaaaa tccctatcac agacaataca
      661 aaggaattgc agaattattg tcttttcttg tttgataagt attatgaagg acaagaagtc
      721 cgccacgtcg gaatcactta cagcaagcta ttttatactg attcgcttca gctggattta
      781 tttagtgatc cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt
      841 cggcaaaaat atggttttac gtcgattgta catgcttctt caatgttaga aggcgctaga
      901 agcattacta gaagcacact tgttggtggt catgcgggcg gaaatggggg aattaaaaat
      961 gattgattac aatgatcgtg gtcgtttaaa gtgggcaggg ttttatttat ctgatcatac
     1021 tgaagagatc gatgcggaca atgagcaacg taccaatcat aatttggcaa aagaacaaat
     1081 gacgactgaa gatattgtga aagtattgaa cgctgcagtc ataaaaaata gatcagtatg
     1141 tatccaaaaa gaagaacgaa acgctgaagg atactatcca tacgatattg ttggaaagat
     1201 cttaggtagt gatgagctgg gaatctacgt tggaaaagac aaaattcact acgacgaaat
     1261 acggcacatt gaattttacc aggataataa gtggagtcga ctagaatgaa cattatccag
     1321 caatatgagc taaagtatat aacatacccg tggtgctagt taattttttc ctagaggaaa
     1381 atgtctaaac atgttggtaa atcaacaaaa aaacgcatat agtagtctca aaactcccaa
     1441 gaaatgagga ctacatatgc gttcatcaa
//
LOCUS       DAGCXG010000214         1449 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGCXG010000214 DAGCXG010000000
VERSION     DAGCXG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.222"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            12..422
                     /gene="spx"
                     /locus_tag="MB022_002805"
     CDS             12..422
                     /gene="spx"
                     /locus_tag="MB022_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4467735.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            436..792
                     /locus_tag="MB022_002806"
     CDS             436..792
                     /locus_tag="MB022_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBT4467736.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          533 a          218 c          270 g          428 t
ORIGIN      
        1 ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
       61 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      121 taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta
      181 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      241 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      301 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      361 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      421 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      481 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      541 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      601 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      661 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      721 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      781 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      841 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      901 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa
      961 aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa
     1021 gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat
     1081 gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa
     1141 acttcaaaaa aagttcttga caaataacaa atgataagtt atgataaatg agttgctgta
     1201 agatttcgaa aaacgaaatc aaaaaaactt ttaaaaaaga gttgacaatt acttgtctct
     1261 ctgatatgat ataaaagttg ctacggcagc caaaaacgaa tcggggaaca ctcctgatga
     1321 agtagacctt tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc
     1381 aattcaagac aacaaacatt ttttaacaag caagcaatat gctagcaaac aaattgagct
     1441 taacaatcg
//
LOCUS       DAGCXG010000215         1421 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.223, whole genome shotgun sequence.
ACCESSION   DAGCXG010000215 DAGCXG010000000
VERSION     DAGCXG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.223"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..1192
                     /locus_tag="MB022_002807"
     CDS             <1..1192
                     /locus_tag="MB022_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4467737.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          563 a          204 c          303 g          351 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat a
//
LOCUS       DAGCXG010000216         1418 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.224, whole genome shotgun sequence.
ACCESSION   DAGCXG010000216 DAGCXG010000000
VERSION     DAGCXG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.224"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(943..1356)
                     /locus_tag="MB022_002808"
     CDS             complement(943..1356)
                     /locus_tag="MB022_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBT4467738.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          518 a          236 c          208 g          456 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctatttt
//
LOCUS       DAGCXG010000217         1403 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGCXG010000217 DAGCXG010000000
VERSION     DAGCXG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.225"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            36..908
                     /locus_tag="MB022_002809"
     CDS             36..908
                     /locus_tag="MB022_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4467739.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1143..>1403)
                     /locus_tag="MB022_002810"
     CDS             complement(1143..>1403)
                     /locus_tag="MB022_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4467740.1"
                     /translation="LDYWNKANLLHSMSKKGYSPDNSACEGFFGRLKNEMFYQRDWKN
                     TTINQFINKLDDYIHWYNNQRIKLSLGGLSPLQYRKKQRYIC"
BASE COUNT          410 a          266 c          319 g          408 t
ORIGIN      
        1 agtgatttca ttctaaaata aagggagaaa tgtctatgct atacggtgca attgaagcag
       61 gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag gaaagagtga
      121 gctttccgac aacgacacct gaggaaacaa tgcctttagt cattgacttc ttcaaacaat
      181 accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg
      241 attccgccac ttatgggtac attacctcga ccccgaaatt ggcatggcaa aattttgatt
      301 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
      361 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      421 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      481 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      541 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      601 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      661 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      721 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      781 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      841 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      901 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      961 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaagatc
     1021 aagccttgaa tgagataaat tcttgctcga aagttatgga aatttcgata gccgtaggcc
     1081 acttgtattg tgcaatagtt ttttacaaaa aaaagggggt gcggatattt ttttggacct
     1141 ttttaacaaa tatatctttg cttcttccga tattgaagag ggcttagccc tcctaaagat
     1201 aacttaattc gttgattatt ataccaatga atataatcat ccaatttgtt tataaactga
     1261 ttgattgttg tattcttcca gtcacgttga taaaacatct cgttttttaa tcgtccaaag
     1321 aaaccttcgc acgctgaatt atctggtgaa taaccctttt tagacataga gtgaagcaag
     1381 ttggctttgt tccaatagtc caa
//
LOCUS       DAGCXG010000218         1386 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.226, whole genome shotgun sequence.
ACCESSION   DAGCXG010000218 DAGCXG010000000
VERSION     DAGCXG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.226"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..250
                     /locus_tag="MB022_002811"
     CDS             <1..250
                     /locus_tag="MB022_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBT4467741.1"
                     /translation="YTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDY
                     LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            255..1202
                     /locus_tag="MB022_002812"
     CDS             255..1202
                     /locus_tag="MB022_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBT4467742.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          456 a          198 c          288 g          444 t
ORIGIN      
        1 atacacatct aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt
       61 taaacctgaa tttctaaaaa aaagtaatta tatttttgat gggcaatgtg ggataataaa
      121 aatgagagat tatctaattt tagatattga tagtgaagaa gattttaaat ggttagaatt
      181 tttgtttcct aaatttttag aagattcttc ggagcttcaa gatatctata attatttata
      241 tcggaggtaa aaggatgagc tgttatctta tcaccggtgg ggctggcttc attggttcta
      301 ccttagccaa tcatttagga gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg
      361 gaaaagtaga aaatcttgat atgacgaaaa atatcacttt tattgaggga gatgtcgctg
      421 agaaatcctt gatggaagag atcatgaaag ctaataaatt tgattatatc tttcatttag
      481 cagcagttgc tagtgtggca gattcagttg cccgaccagt agaaacacat cgtgtgaatt
      541 ttgaaagtgt attaatgatt ttagaattga tccgcaagta tcaacctgat ttaaaacgga
      601 tcgtgtttag ttcttcagcg gctgtttatg gggatgaacc gactttacca aaaaaagaag
      661 agtccgtgat tcgcccattg acgccttatg cggttgataa attcgctgca gaacaatatg
      721 tgctagatta ttgtcattta tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct
      781 atggtccaaa tcaaaatccg aattctccat acagtggtgt gatttctatt ctcgtagatc
      841 gctataagaa acaaatcgta ggagaagcaa cttcatttac attatttggt gatggaagcc
      901 agtctagaga ctttgtttat atagatgatg taatccaagc attactttta gttgcgaatg
      961 aggaagctgc actggggaaa caattcaatg ttggaacggg taaagcaacc acacttttgg
     1021 aattgattca tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa tacaaagaag
     1081 agcgttcggg tgatatccat gactctctag cagatattac taagatccag tcaattggct
     1141 atcaaccgaa atatggtatt ttaagtggaa tgactaatta tttgaaaact gaaatcaatt
     1201 aaaacatact aagataagat tagagggtaa attcaggctc tttgtcaata aggactgatg
     1261 agttttgtaa aatcaaatct gggatagaag ggctctttgt caaatgatgt tggtaaagag
     1321 aaatcttaga ataatttgga cagaacagaa agaagaaaac ctctttctgt tctgtttatt
     1381 tggtta
//
LOCUS       DAGCXG010000219         1384 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGCXG010000219 DAGCXG010000000
VERSION     DAGCXG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.227"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(72..479)
                     /locus_tag="MB022_002813"
     CDS             complement(72..479)
                     /locus_tag="MB022_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467743.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(498..1316)
                     /locus_tag="MB022_002814"
     CDS             complement(498..1316)
                     /locus_tag="MB022_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4467744.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          403 a          232 c          192 g          557 t
ORIGIN      
        1 gtataactta tactttaata ttcatttatc tagtataact tatactttaa ttgaataccg
       61 catgaaaaaa attattttgg taatttttca aactcttctt gtatttcttc tcttatgtgt
      121 tcttcttttg ctactcctag tctatgctgg atatatgagt ctgctaatgt attaacaatt
      181 ttttcgaaag aaattctccc tttttcactt tgtaactccg tcatatagtc ttgtaaaatt
      241 gagagtttgc tatttaatgc cggagagatc tttttaggaa ctgatacctt gtttctttta
      301 tcaggaattg aaactggtcg gcctcttttt ttcaatgtaa tatttcttgt gttactcaat
      361 gcactattgc taaagttata ttcaccgctt attttcacat cttggcgttt gttattgggt
      421 aataatggct caaattcacc attctccatc attttaggaa tatcaaaatc tttaggcatt
      481 acttatgcaa ctccttatta tttatttaaa aactcttgtc tagcatctag ttctgccaaa
      541 atatttataa atacttggtg tgctttataa tcccattgat caaaatttcc atttttgtat
      601 tttcttaaat gtataccaat tttagaatac cttctcaatc ttttttgata atgaatgaga
      661 tttttggata ccacggttcc gtatttttca attgcttctt ctaggatttc ttcatcaaaa
      721 tcatcatttg gttctaccat acaaggtaca atatctataa tttgcaaatc aagatcgtat
      781 ctatcgacca tgaatttttg ataatttaca tatttgttga ctccctctaa ggattcttca
      841 gctgtttgaa atgaaattat cgaatagtct gaagctgcca tagcattatc ggaaaattcg
      901 ctaatggttg gtggtacatc aataaatatt tcttgataat tgttctttat tggaagaagt
      961 aattttttca agacagtaac ttggtctaat tcattttcat gtttattagc aaaagcggag
     1021 aatgatcgaa aactagtgtc acatgccaga aggtcaagat tttcatttac cttaataata
     1081 ctacttgcta attctccatt tgcgacacca tttgttatag tggttttgat atcggttata
     1141 ccgaaagttc ttgctatgac ttgtgttaag ttcgcttgag ggtctaaatc aataataagc
     1201 gtttttctat ttcttatcat tgcattgtca taagcaagca tgctgactat cttagtttta
     1261 cctactcctc ctttaaaatt gccaactgta aatgtagtcc cttgaaaagt atccatgtaa
     1321 atcctcccaa tctttaattg ataattaaag tataacttat atatcggaaa tggtcaacat
     1381 taaa
//
LOCUS       DAGCXG010000220         1368 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.228, whole genome shotgun sequence.
ACCESSION   DAGCXG010000220 DAGCXG010000000
VERSION     DAGCXG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.228"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            82..>1368
                     /locus_tag="MB022_002815"
     CDS             82..>1368
                     /locus_tag="MB022_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBT4467745.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAGCXG010000221         1368 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.229, whole genome shotgun sequence.
ACCESSION   DAGCXG010000221 DAGCXG010000000
VERSION     DAGCXG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.229"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            82..>1368
                     /locus_tag="MB022_002816"
     CDS             82..>1368
                     /locus_tag="MB022_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBT4467746.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIK"
BASE COUNT          466 a          256 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaaa
//
LOCUS       DAGCXG010000222         1364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGCXG010000222 DAGCXG010000000
VERSION     DAGCXG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.230"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(140..1288)
                     /locus_tag="MB022_002817"
     CDS             complement(140..1288)
                     /locus_tag="MB022_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467747.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAGCXG010000223         1362 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.231, whole genome shotgun sequence.
ACCESSION   DAGCXG010000223 DAGCXG010000000
VERSION     DAGCXG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.231"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            12..986
                     /gene="bsh"
                     /locus_tag="MB022_002818"
     CDS             12..986
                     /gene="bsh"
                     /locus_tag="MB022_002818"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4467748.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVVEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          473 a          182 c          226 g          481 t
ORIGIN      
        1 aggaataaac tatgtgtacg tctattactt atgtaacaag tgatcattat tttggaagga
       61 attttgatta tgaaatatct tacaatgaag tagtcactat tactccaaga aattataagt
      121 tgaattttcg aaaggtaaat gatttggata ctcattatgc aatgattggt attgccgctg
      181 gtatagctga ctaccctctt tattacgatg cgacaaatga aaaaggattg agtatggctg
      241 gactaaattt ttctgggtat gctgattata aagaaataca agaagggaaa gacaatgtat
      301 ctccttttga atttattcct tggattttag gacaatgctc aacagtagta gaagctaaaa
      361 aattgttaaa aaatatcaat ttagcaaata taaattatag tgacgaactt cctttatccc
      421 ctttacattg gctattagct gataaagaaa aatcaattgt cattgaaagt atgaaagatg
      481 gacttcatat atatgataac cctgtgggcg ttcttaccaa taatccttca tttgactatc
      541 aattatttaa tttaaacaat tatcgtgtct tatcgagtga aactcctaaa aataattttt
      601 caaatcaaat aagtttgaat gcctatagcc gcggtatggg agggataggc ttgcctggag
      661 atttatcctc agtatctcgt tttgttaaag cgacttttac gaagctgaat tctgtatctg
      721 gagattcaga gtcagaaagt attagtcaat ttttccatat cttaggttca gtagaacaac
      781 aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata tacaatttat tcttcttgtt
      841 gcaatgttga caaaggaatc tattattatc gaacatatga agacagtcaa attactgcaa
      901 ttgatatgaa taaagaagac ttagatagtc ataagttaat tagttatcca attatagaaa
      961 aacaacaaat taaatatata aattagttaa tgtgttgtga ttgattattt aatatagata
     1021 taatacaaaa aggcaaagat attttcaaaa cattttttct tgatgtgata tccttgtctt
     1081 ttttgtaatg tttttaactt attgcttttg caaaatttat ttacctgttt tctggatcaa
     1141 cgatagtgta tattatgcta tccttgtttg gattttagga cgtttaaaga ggcacttttt
     1201 tacataactt aatccataga ccttttaagc ctgctgtgag ataataataa agaagagttt
     1261 aaagcgcacc ccaaaccact tccccataag tgtattacgc tttaaactct tttatatttg
     1321 aattcactaa gaagcatacc atatttttga aaaaaagtga ga
//
LOCUS       DAGCXG010000224         1332 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.233, whole genome shotgun sequence.
ACCESSION   DAGCXG010000224 DAGCXG010000000
VERSION     DAGCXG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.233"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>1332)
                     /locus_tag="MB022_002819"
     rRNA            complement(<1..>1332)
                     /locus_tag="MB022_002819"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          280 a          413 c          307 g          332 t
ORIGIN      
        1 atccagccgc accttccgat acggctacct tgttacgact tcaccccaat catctatccc
       61 accttaggcg gctggctcca aaaggttacc tcaccgactt cgggtgttac aaactctcgt
      121 ggtgtgacgg gcggtgtgta caaggcccgg gaacgtattc accgcggcgt gctgatccgc
      181 gattactagc gattccggct tcatgcaggc gagttgcagc ctgcaatccg aactgagaga
      241 agctttaaga gattagctta gcctcgcgac ttcgcaactc gttgtacttc ccattgtagc
      301 acgtgtgtag cccaggtcat aaggggcatg atgatttgac gtcatcccca ccttcctccg
      361 gtttgtcacc ggcagtcttg ctagagtgcc caactgaatg atggcaacta acaataaggg
      421 ttgcgctcgt tgcgggactt aacccaacat ctcacgacac gagctgacga caaccatgca
      481 ccacctgtca ctttgccccc gaaggggaag ctctatctct agagtggtca aaggatgtca
      541 agacctggta aggttcttcg cgttgcttcg aattaaacca catgctccac cgcttgtgcg
      601 ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg cggagtgctt
      661 aatgcgttag ctgcagcact gaagggcgga aaccctccaa cacttagcac tcatcgttta
      721 cggcgtggac taccagggta tctaatcctg tttgctcccc acgctttcga gcctcagcgt
      781 cagttacaga ccagagagcc gccttcgcca ctggtgttcc tccatatatc tacgcatttc
      841 accgctacac atggaattcc actctcctct tctgcactca agtctcccag tttccaatga
      901 ccctccccgg ttgagccggg ggctttcaca tcagacttaa gaaaccgcct gcgctcgctt
      961 tacgcccaat aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt
     1021 agttagccgt ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg
     1081 ttcttctcta acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct
     1141 cggtcagact ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg
     1201 ccgtgtctca gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt
     1261 ggtgagccgt tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc
     1321 gaaagcgcct tt
//
LOCUS       DAGCXG010000225         1324 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.234, whole genome shotgun sequence.
ACCESSION   DAGCXG010000225 DAGCXG010000000
VERSION     DAGCXG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.234"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(293..907)
                     /locus_tag="MB022_002820"
     CDS             complement(293..907)
                     /locus_tag="MB022_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4467749.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          431 a          262 c          220 g          411 t
ORIGIN      
        1 agagacaaga aagaatccat atggattctt tcttgtctct ttttccacta ctttttattt
       61 ttctgacact acatattctt ataactcatt tctcataact gtgaaacaac ttataaaaat
      121 aacaaacttt aaacaaaaca agcttaaaat caaagatgat ccatcttaaa ataactaaaa
      181 caaaaaaagt agtcgttgtt aatcctcatt aataatcgtt ttcttttttt caaacgctcc
      241 atttttatgt ggaacattat ttttttaagg aactttgtca acatgtggat aactattttc
      301 cacttgtgtc ctggactagc tgttttgctg taaaatacaa aacttgctgc agcaaaaaga
      361 tcgttgcaag agaggctgtt ccgcagatcg ctggatctag atttggcaaa gtcaagactg
      421 catcatgtaa attggcgtgg ttgtccaaaa ccaaatcagc aaattcaaat aatcgcaaac
      481 cactgctatg ttgcgatttg atacttcgtg acaaatcaaa tccagtaaca atgattaatg
      541 gatgaccatt ttctttgatc cactgagcta gttcgatgat tgccgggttt cttccttcat
      601 tcgaaagaag aagaaaaatg tcttctgatt tgacagaaag ctgatcaata attgcctgtc
      661 ctgcaccttc tagcgtttca tagacacctc cggctggatt ttcaatcaat atacttggta
      721 caagatccgg ggtttgatcc caaaattcaa atgctacacc acttagtgtg cggctagcat
      781 atatctgcaa tctaccacct tgattaattt gtttcgcgat ccaagtgcca gcttgaagca
      841 tcagcggctc ttctatcctt aatttctctt ccaattggtg ataatagtca aaataacata
      901 aactcattta tctctcccgc ctagctatac ttgacatgaa tagaaacagc tgcctaatct
      961 aaggaagtac ataaaaaaac agtagttaga cagtaacaca tgtggtcttt gctgtttcaa
     1021 acacaaaaaa ggcattgaac gaatacgccc aataccttga catagcaaat aaaacaacgt
     1081 gcagaccatg gaaaatatct acataggggt agaattttca ccctagatta ggtacaataa
     1141 attaggtcga tgcactgcat tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga
     1201 ctggtcattc cctgattctt agcgccaact aagggtcagg gagccttttt ttattcagct
     1261 gtttctatct gaatttattg taagtaatct cttatccaag tgcaagcaag aaaatacaaa
     1321 aaaa
//
LOCUS       DAGCXG010000226         1324 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.235, whole genome shotgun sequence.
ACCESSION   DAGCXG010000226 DAGCXG010000000
VERSION     DAGCXG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.235"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            278..820
                     /locus_tag="MB022_002821"
     CDS             278..820
                     /locus_tag="MB022_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467750.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="MB022_002822"
     CDS             976..>1324
                     /locus_tag="MB022_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBT4467751.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          247 g          387 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc ttgatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAGCXG010000227         1309 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.236, whole genome shotgun sequence.
ACCESSION   DAGCXG010000227 DAGCXG010000000
VERSION     DAGCXG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.236"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1003)
                     /locus_tag="MB022_002823"
     CDS             complement(<1..1003)
                     /locus_tag="MB022_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4467752.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     "
BASE COUNT          389 a          260 c          202 g          458 t
ORIGIN      
        1 gtattttcat ctggtgtgaa aatcctggtc tcgtttctaa gatcgacgta ccaatgttta
       61 aatgaacaca tcctgtttta caattatgct gcctgatacg aatggctact tgttccgcca
      121 tttcttttac caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg
      181 aattaccata agatttttcc tttgtcggtt ctggcgggat tgctatatct gttcgatcga
      241 ttccgttagc atggaagtac agctgtaatc caatcacacc taatctattc ttaatcgtat
      301 aaggattcca gttagcaagg tctctaatgc tcaaaattcc catttggttg agcctttttt
      361 tcattcgaga accaattccc caaaagtccg tcatttcggg aatattccat actttctcag
      421 gaacgttttc gtacgtccat tccgcaataa atccttcatt atgctttgct tcattgtcta
      481 aggctaattt agccaaaagg ggattatcgc cgacgcctac cgtagcaatc aaacctaact
      541 cttctttaat acgttcttgg atcatttgag cgagcttctt ccttcgctga cttcgtgttt
      601 cttcagtcgt aaaaagattc agtgattttg tcacttttaa aatcgattca tcgatcgagt
      661 aaatcagtag atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc
      721 gcttgatata taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg
      781 gtaagtcacg tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag
      841 aagctaatat caatcctgaa ccagtattat cagatcgact cataacaaca agttctgttg
      901 ttaatggatc taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga
      961 ttaaaaagta atcatttact ggctcttttg aataatccat cataaataac actccattct
     1021 tccacattat acaaacatat gttcgatgtt gtaaagaaga aatagctaaa tacataaaaa
     1081 aagaagcata ggtatgctat caccctgctt cttcttatga tttgtatgat caatttattc
     1141 tatcacttta ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag
     1201 actgcaaatt agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata
     1261 aaatccttac aacatttgat aataagttat cagtagacac ttacaaaaa
//
LOCUS       DAGCXG010000228         1266 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.237, whole genome shotgun sequence.
ACCESSION   DAGCXG010000228 DAGCXG010000000
VERSION     DAGCXG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.237"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>1266
                     /locus_tag="MB022_002824"
     rRNA            <1..>1266
                     /locus_tag="MB022_002824"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          334 a          283 c          386 g          263 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagta
//
LOCUS       DAGCXG010000229         1250 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.239, whole genome shotgun sequence.
ACCESSION   DAGCXG010000229 DAGCXG010000000
VERSION     DAGCXG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.239"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            18..491
                     /locus_tag="MB022_002825"
     CDS             18..491
                     /locus_tag="MB022_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBT4467753.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="MB022_002826"
     CDS             500..727
                     /locus_tag="MB022_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4467754.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          396 a          196 c          209 g          449 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1141 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     1201 acagtgatgg acaattcttt tttattttta acattatttt gataataatg
//
LOCUS       DAGCXG010000230         1223 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.240, whole genome shotgun sequence.
ACCESSION   DAGCXG010000230 DAGCXG010000000
VERSION     DAGCXG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.240"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..1161
                     /locus_tag="MB022_002827"
     CDS             <1..1161
                     /locus_tag="MB022_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4467755.1"
                     /translation="LIPLTFFVELFTDVSAAIPIIGSYFILKEKKKTNIVLLNKLLLC
                     MLIDDFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVE
                     QSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITI
                     RQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSIL
                     EKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTI
                     IEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLS
                     IDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          446 a          138 c          196 g          443 t
ORIGIN      
        1 ttgataccac tcactttttt tgttgaatta tttactgatg tttccgcagc tatacctata
       61 atcggaagtt attttatatt aaaggaaaag aagaaaacta atatagtgct tttaaataaa
      121 ttactattat gtatgcttat tgatgatttc agatctatta ttacttccat ggttatgacg
      181 tatatgtttt catttgaagg gataaaaggg tatggttatg tagtattcca gataatgctt
      241 gattctttgt ttttgctgat gtttatttgg ttatatagaa aattagctat aaataggatt
      301 gttgaacagt ccagttctaa acttactgct atattcatga tttatttact tacaattggt
      361 ttatttgttt cttatgttgc tcatcaatat caagtatttg atcattttgt gtttggtgtc
      421 ttaatatttt taatcattca aatgtttgtt gttctatttt tatttattag aataactatt
      481 aggcaacgag aacaatatga acagcaattg aaaagaaaag aattggttta tctaaaaaaa
      541 tatactgatt cactggaaaa agatcaagaa aaatttgcca gatttagaca tgactataaa
      601 aatttattgc tgagtttaaa agaaatcgct aatcaaaacc atgattcgat attggaaaaa
      661 gaaataaaag aattagagat ctattctaat tcttatctaa ccaataagtt tgaatatcgt
      721 tatttaaaaa atataaaaaa tgaatatttg aaaagtttgt tgatagcgaa gctataccaa
      781 gcaaataaca ataagatttg ttgtaaattc gaatgtccaa gtactattat tgaagttcct
      841 gttccaatat ttgactgtat tagggtgctt ggaattattc tcgataatgc aattgaagca
      901 tctatagaga gtcaagatag agaactgtct ctagctattt accgagatga taaacagata
      961 gaaatttcga tatcgaatag ttgtagagag atactactct ctatagatac tctaatgaaa
     1021 aaaggaataa caacaaaaaa gggacatcaa gggttaggtt tatctagtat agaggaaatc
     1081 aataaaaaga atcccaatat gtttgtgaac tatcaaaacg atccgtatca atttactact
     1141 caagttattt tgttgttttg agggaaggag gatttatgag ttatccaata atattatgtg
     1201 aagatcaaat tatacaatta aat
//
LOCUS       DAGCXG010000231         1166 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.241, whole genome shotgun sequence.
ACCESSION   DAGCXG010000231 DAGCXG010000000
VERSION     DAGCXG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.241"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            316..570
                     /locus_tag="MB022_002828"
     CDS             316..570
                     /locus_tag="MB022_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4467756.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="MB022_002829"
     CDS             571..888
                     /locus_tag="MB022_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4467757.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          404 a          196 c          236 g          330 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gacttt
//
LOCUS       DAGCXG010000232         1166 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.242, whole genome shotgun sequence.
ACCESSION   DAGCXG010000232 DAGCXG010000000
VERSION     DAGCXG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.242"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..517
                     /locus_tag="MB022_002830"
     CDS             <1..517
                     /locus_tag="MB022_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467758.1"
                     /translation="VVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVG
                     IILFFVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHES
                     TVKGIRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWN
                     SSDLELPMKDK"
     gene            538..821
                     /locus_tag="MB022_002831"
                     /pseudo
     CDS             538..821
                     /locus_tag="MB022_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          449 a          140 c          238 g          339 t
ORIGIN      
        1 tgttgtaaat gcaattctag cgactttaat aaaacaaatt gaacaatctg gaagaacaac
       61 aaaaggagtg aaggattttt tattgacgtt tattcacttt gtaaatggac acatgtattt
      121 gtttgaagtg ggtattattc tcttttttgt cgggtatgta tccacgaaac ttattaaacg
      181 ttcaattgaa aaagaaattg aaattgacct gattccgaca cttcttggtt atgagtacaa
      241 tgttgatttt ggaagatatg acgaattgat caatacggat atcgttcaca cgagtatcca
      301 tgaatctact gttaagggca tcagatctat tttaaaaaat ggccaggaaa tgaaagcaat
      361 agatcgaaga aaagagatct acaattttta tgaagtcgaa gcaagcctac gatatggcat
      421 cggaaaattg tggttagatg gaagtaatat tcgtcaaaac aaaccataca ttttttggaa
      481 cagtagtgat ttagaacttc caatgaaaga taaataatta tggagaatcg tttgaaaatg
      541 attcctctat acaggattgt gcttatagtt ggcatggagt taaaaacctt aaagaaggag
      601 cagttattca aagtgaaaga acgtcataat cagttactat gtagtaaaac gggtacgatg
      661 aacattgaat accgaacaga atatttagaa ttaacgaatc aaactgattt tttgttgatt
      721 cgaaagcaac aagagcaaga aaaaaattaa tggctactta tagccaattg attgatgaat
      781 gggaacaatt caaggaatct aaaattccag aacgtctcta aaagccttta tttgtaaaga
      841 gcataaggaa acgtgggaac actttttaaa aaaagtgttc caataggctg tgcctattac
      901 gtttctcaaa aataaatgtg ggaacaaaat atttaaaaat aggatagtga aaggatagtg
      961 aaggatagta aaaggagggt agtaggatag caaaaaggat aggctccttt tcgctatcct
     1021 ttttaaattg gaggaattaa tatggaagta agaataagaa atgtggatga gaaaatcgta
     1081 agagaaattg atcgaagatg tgacgaacgg ttctgttgca aagttttaaa tctactatca
     1141 aataaggtag aataatagaa aaagat
//
LOCUS       DAGCXG010000233         1144 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.243, whole genome shotgun sequence.
ACCESSION   DAGCXG010000233 DAGCXG010000000
VERSION     DAGCXG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.243"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            29..583
                     /locus_tag="MB022_002832"
     CDS             29..583
                     /locus_tag="MB022_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4467759.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            837..>1144
                     /locus_tag="MB022_002833"
     CDS             837..>1144
                     /locus_tag="MB022_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467760.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          467 a          116 c          211 g          350 t
ORIGIN      
        1 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
       61 tgaagagata caagattttc agttttggct aagaagaaca agtaatccag aaagagatag
      121 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
      181 agttggagac atacgaggta aaagtaagcc tattattgtt gaacataaaa caggtaagcg
      241 aaaaccgatt tttatagata atttgagaga agagatttta ttatatactg aaggtaaaga
      301 agaaaatgat tggttatttc caagtcggca acaaggtcga catataacaa gggatcgtgt
      361 ctatcagata tatgcagata tagcagaaaa attaggaaga gatgatatag gaacacatac
      421 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
      481 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
      541 tgaaataggg gcatcgttac gtggttttaa attgggaggc taattgagat attaggatat
      601 attttttgtg tataatgaaa acttagaagg tgagattaat gaagataact gaattttcgg
      661 aagtatttaa aacgtttagt aacatttaca atagaatacc agagatagaa caaaatgttg
      721 tagttgataa tgattattat atagagaatg attttaataa accaagaaaa tatgacaacc
      781 tatacaattt tatacacaat agaacaacaa taaataacaa cagtaacact cgagttttga
      841 ttaattcagc atattttcat attaaatatt taaagaaata tgagtatgaa gaagttgtat
      901 tagattatgg gctaaataga cagtcatttt taatttatgc aataaataac ggaaatttgc
      961 caagcaataa aaagaacata attattgaaa catatgccta tctggagggg ggatctaaaa
     1021 aactaattaa gtcatgtaat atagagtatc aattatcagt aggagaaatt aaatgtgttt
     1081 tcgaagaaga tttagaactt gacttaaaaa aaatttttgc cagcattcat gattatatag
     1141 gttt
//
LOCUS       DAGCXG010000234         1122 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.244, whole genome shotgun sequence.
ACCESSION   DAGCXG010000234 DAGCXG010000000
VERSION     DAGCXG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.244"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..104
                     /locus_tag="MB022_002834"
     CDS             <1..104
                     /locus_tag="MB022_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBT4467761.1"
                     /translation="GTVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..724
                     /locus_tag="MB022_002835"
     CDS             104..724
                     /locus_tag="MB022_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467762.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            728..>1122
                     /locus_tag="MB022_002836"
     CDS             728..>1122
                     /locus_tag="MB022_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467763.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATD"
BASE COUNT          404 a          188 c          253 g          277 t
ORIGIN      
        1 ttggaactgt agaattcccg attgaagaag gagccttgat catcgcaacc gttgatgatc
       61 caaagcaaaa tgtgttcact cttgagaaag tcggggtgat ctaatggcta aaactaaacg
      121 aatgatggaa acggatgaaa aaacaggtgt acaacgccaa ttctttccaa tcacacatgc
      181 ttccgcagtt cttgggttag aagaaataat agcaggagaa gcaacagttt tatctgttaa
      241 tggaaaaatt ggagccgtcg tcattactaa agaagatttg ggattagaga atgttctcac
      301 agaattaccc tatgcaagtg aagaagatga cggcattatc acggcagaaa tgtatcaaaa
      361 aattttaaac agtggagaag gtgactacgt gttgccagtt gccactatcg accgtttggg
      421 tggcataaaa gttggtgaat tattgacgat tgatgagaca gggaaagtct ctgcagtcag
      481 acaatctgat gtcaattttt cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta
      541 tactgctgga gaaaacatca ctattgatga agatggaaca attagttcta caggtggtag
      601 tgggactggt ggtgtcaatc agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca
      661 agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa ttggggaggt
      721 atagagaatg acagatatcg taaaaataaa acaaagcaat gtacaggttt atccacaaac
      781 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
      841 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      901 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      961 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
     1021 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
     1081 tgctacaact acgacagcag gtttaatgtc agcaaccgat aa
//
LOCUS       DAGCXG010000235         1091 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.245, whole genome shotgun sequence.
ACCESSION   DAGCXG010000235 DAGCXG010000000
VERSION     DAGCXG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.245"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            143..400
                     /locus_tag="MB022_002837"
     CDS             143..400
                     /locus_tag="MB022_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467764.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(594..>1091)
                     /locus_tag="MB022_002838"
     CDS             complement(594..>1091)
                     /locus_tag="MB022_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBT4467765.1"
                     /translation="KLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGV
                     HDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSL
                     LGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLR
                     YSLGY"
BASE COUNT          393 a          166 c          177 g          355 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactctga tctattaaac gactcattat
      421 gagtcgtttt tttatacaaa gaaactatct ggaggtaatc caaatataaa tattgattgt
      481 agtacaaaaa agaagcgtcc gaagacgctt cgctgtttaa atgcgtaaaa agattgggaa
      541 tttgattcat cagctattac gactattatt ataacccgtg gtgctagtta attttaatat
      601 cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa tagaacactg
      661 taagccagta atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa
      721 ttttgacaac ctaatttttc taaggaagaa aaaacagtct caactgtttt tctttgtttt
      781 ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg cggaacggat
      841 atagtaatgc ccttaagggc aagcttttca tggattttat ggctaatata tcctttgtcc
      901 cctaggatat tgggaagatt cgcttcttcc gataaagtct ctaacacttt cacgtcatgg
      961 acacctggat tcgtgattga gtaagtaatc ggaaagccag tcttagtaac gatcatatga
     1021 attttaagac cataaaaata agattttttt gttgagttat agccaacttt ggctatttga
     1081 ttcaatagtt t
//
LOCUS       DAGCXG010000236         1082 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.246, whole genome shotgun sequence.
ACCESSION   DAGCXG010000236 DAGCXG010000000
VERSION     DAGCXG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.246"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            109..>1082
                     /locus_tag="MB022_002839"
     CDS             109..>1082
                     /locus_tag="MB022_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467766.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYP"
BASE COUNT          426 a          152 c          185 g          319 t
ORIGIN      
        1 tgaaataatc aagaatatta gaaacttcta cattaatcaa gaactttctt ctgtatgaaa
       61 atagagataa taattgtaca cttaagctgt attcaggagg tgaagataat gggcatcgaa
      121 tttagcgaac ggcaaatgaa aatactatac tttttgattg aaaatgagca cacaacttac
      181 aaagaattat cagaacaatt atatgtatct acgaaaacaa ttagcaaaga tattttaatt
      241 ttgaaagaaa taatccaaaa aatgccagat gtacaaatgt tggttcaacc taaaaaaggc
      301 gtttcgctta ttggaaaaaa agaaacactt cgaaatttat tatccagtca taaaatcaat
      361 atcgcagact ctccggagga ccgtctaact tatgtgtttg ctaaattatt gaatactaat
      421 gattctctaa agattcaaaa tcttgctgac gaattatacg tgagcaggtc gacggtagaa
      481 agcacaatga aagatgttag aaaaagacta aacaagcatg ggatgtcctt agtttcagat
      541 cgaaatggcg tgcgcatcaa agctgatgaa aaaagtaaaa gaaggttgat gtcagaagtt
      601 gtgaactatt attggggtgg ctttacagca tctaacagta aaacagatct cttagacttg
      661 aatatcaatt tcattggtga agcgaataat ttcttaaata aggacatatt gaaaaaagta
      721 gccgaattgc ttaatcgata catgaataag acacaattga aatttacaga ttatgaattt
      781 caaactttag ctatccacct tgctattgcg atagaaagga ttgaaacaag caatttctta
      841 tcacgtagta ataattctga agaagaaatc tcaaagaata cacttatttt aatcaaaatg
      901 attgaaaaag cattttcaat tattgtgcca atagatgaac aagcttatgt tgacattcac
      961 attacttcga ttgaaaaaaa tgctatgaat cacgacttag atcaaacgaa gctttcctat
     1021 ttagatatgg gtgttgagct acaagagtta gtaagaatta aattaaatga attttatcct
     1081 ga
//
LOCUS       DAGCXG010000237         1069 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.247, whole genome shotgun sequence.
ACCESSION   DAGCXG010000237 DAGCXG010000000
VERSION     DAGCXG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.247"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..969
                     /locus_tag="MB022_002840"
     CDS             <1..969
                     /locus_tag="MB022_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4467767.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAGCXG010000238         1055 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.248, whole genome shotgun sequence.
ACCESSION   DAGCXG010000238 DAGCXG010000000
VERSION     DAGCXG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.248"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(250..798)
                     /gene="aac(6')"
                     /locus_tag="MB022_002841"
     CDS             complement(250..798)
                     /gene="aac(6')"
                     /locus_tag="MB022_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4467768.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          315 a          217 c          157 g          366 t
ORIGIN      
        1 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
       61 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
      121 ctggtacacg gtgttccaaa agtagcttct tcggaaataa gttggaattt cacaaaaatt
      181 caagaagtaa ttttcgtaaa ttccttctta tttcttgaag ctgattactt ctgtcacaac
      241 ctctttttat tattgagaat ttggtcgagg aataatcgtt tttgccatcc aaatatccgg
      301 tttgtcccag ccatttgcat ttggtaagac acctacgatt ttataaccta atttttcata
      361 gaattcatac ggatgttcac gaaggttctg gatggaagcc actttatcaa atgtatgctc
      421 atacaggtcc gtttgactta acgttgttcc atggtctaaa tcatccgtac ctaaataaat
      481 cgtgattcct cctctggaag ctacttcttt ttctaagtaa ttgactaatc gagtacctat
      541 ttggttcttt cgtcgggagc tttctacaac taatggatgc aattcccaac ctgtgatacc
      601 gtattgaggg attgcaccaa taaatcctac taactcatct tggtctaccg ctgctaccgc
      661 gattcgttct ggattcatca tttcttctac ttcttctgcc gagctgtctc catattcttc
      721 cggccaagtc agtctcagta aatcagaaag ctgatctttc aatactggat tattacggtc
      781 aaattcactg attatcatac gtcttccccc ttgttcttgc ttattctaac agtccttttg
      841 aaaaaacgaa atgttctcgc tcaaaaatct ttagctacca atcagtatct ctattctttc
      901 attatttatc ttacttataa aataaaaaaa ttatacagaa acactttatt caaaatagga
      961 aagatcagga aagccatcaa ttggaggaaa aaactctgtt tgttcatcat gatatagatt
     1021 atcttttgct ttaaaaaaaa ccaattatga attga
//
LOCUS       DAGCXG010000239         1047 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.249, whole genome shotgun sequence.
ACCESSION   DAGCXG010000239 DAGCXG010000000
VERSION     DAGCXG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.249"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..788
                     /locus_tag="MB022_002842"
     CDS             <1..788
                     /locus_tag="MB022_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBT4467769.1"
                     /translation="KIEDLFHEPLIEYTGNLSKTIEQIKIDNLLSQNYDPEIYLFLYE
                     NKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGM
                     DIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQF
                     IGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHT
                     ISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          396 a          118 c          183 g          350 t
ORIGIN      
        1 gaaaaataga agatctattc catgaaccgt taattgaata tacaggaaat ctaagtaaaa
       61 ctatcgagca aatcaaaata gataatctgt tatcacaaaa ttacgatcca gaaatatatc
      121 tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt cgaaatttag
      181 aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga gatacgattc
      241 ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg tctataacat
      301 taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa gaaaacgaat
      361 tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt tattatacta
      421 aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct gtcgttcaat
      481 tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat tatttttctg
      541 taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg atttataatt
      601 atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac catactatca
      661 gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga gtattcaaaa
      721 agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggataat ttcaacaaca
      781 ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt ggaggagttg
      841 aggaaattgg attttaacaa aaaatttttt gggaaatttc tgatttgagt tgtttcgtag
      901 aatctgagcg tctaagtaat ggtaataata gtgtgttaaa tgaatatgac aaagtagtaa
      961 ttaataaagt aaaaagttta tgtgaagaag ctagagaatc tatcagtcat attaatggta
     1021 gcaattttaa gcaaacatta tttaatc
//
LOCUS       DAGCXG010000240         1037 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.250, whole genome shotgun sequence.
ACCESSION   DAGCXG010000240 DAGCXG010000000
VERSION     DAGCXG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.250"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..114)
                     /locus_tag="MB022_002843"
     CDS             complement(<1..114)
                     /locus_tag="MB022_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBT4467770.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
     gene            187..465
                     /locus_tag="MB022_002844"
                     /pseudo
     CDS             187..465
                     /locus_tag="MB022_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
     gene            667..1037
                     /locus_tag="MB022_002845"
                     /pseudo
     CDS             667..1037
                     /locus_tag="MB022_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          399 a          154 c          187 g          297 t
ORIGIN      
        1 acgatcatat aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaacc
       61 aacagcgaca ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct
      121 gtcctctctg tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa
      181 cagaaccaat atgaaaaagt acaaatagtt gatgttaata atggaaatag atttgaaact
      241 tatacaatag ctggagagag aggaacaggg atgatttgct tgaatggagc tgcagctaga
      301 tgtgtatcaa ctggagataa aataatttta atggcatatt gtgaattaaa tgaaaatgag
      361 gttaaggatc atagacctaa ggttgttttt gtcgatgata ataataaagt tgaaagagta
      421 acatcatatg aaaaacatgg aaggttatca gatataattc tttaggtttt gtaatgttat
      481 ttatatatag ctagactatt attgaaatat ttattgatta ctaattggat agatgtcaat
      541 aaagtagaca caaaaagaga agatacagac tgtagacaaa gtccatttta tgtgaatcgg
      601 ctacactaga ctagacagaa aaaataaggt gtgtagaata ataaaaaaca cactggagga
      661 tttttcatgt caagacgtca acgaagaacc tattcaaaag aattcaaaca acaaatcgtc
      721 aatctctatc tcgctggtaa gcctcgcgca gaaattattc gagagtatga gcttacgcct
      781 tcttctttcg ataaatggat gaagcaagca caatcaacgg gctcattcaa aaaaagagac
      841 aacttaacac cagaacaagc agaattgatc gcactaagaa agaaaaataa gcaactcgaa
      901 atggagaatg atattttaaa gcaagcggcg ctgatattcg gacgaaaaga caagtaattg
      961 atgccaacaa gcataaatat tccatatcag cgatgtgcaa aattctaaat atttctcgtc
     1021 agacctacta ttatcaa
//
LOCUS       DAGCXG010000241         1021 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.251, whole genome shotgun sequence.
ACCESSION   DAGCXG010000241 DAGCXG010000000
VERSION     DAGCXG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.251"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..994)
                     /locus_tag="MB022_002846"
     CDS             complement(<1..994)
                     /locus_tag="MB022_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467771.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFI"
BASE COUNT          426 a          150 c          161 g          284 t
ORIGIN      
        1 ctataaataa agaagaaaaa ccatttaacc gccagacaaa ttgtagactc ttaactggtg
       61 tatggttaaa aagttgccaa ggaaataaat tggttgaaca aaaaagaaca aatacaccaa
      121 gaaagaaaat aaataaatca tcagcagtta atttttttat aaaaataaaa gcaccaatca
      181 atccgagaaa taagaataat ccaatagtat agttgttata gttattattt aaaatatgtg
      241 tcagcatatc actaggagcc ataccactta attcttttcc cagtgggact ttcaattctt
      301 gtgctctaga ttgttgaata aaaggaatta agaatcccgc acttaaaaaa aaggtgagga
      361 cggtagcttt taaaagactt aagagtctag cgattttttg gtcccaaaaa taaaaagaaa
      421 gaaacaacgt tcctccaata aatacagaaa ccatagcgac agaaagtaaa tgagtataca
      481 ctactagcgt catgccaata gacaaccagt accatttttg gtaatctcta atataaattt
      541 cgtaacaccc cattagtatc aaaggcaaaa atgttaaagc gacagcttct cccaatgaag
      601 cacgatggaa tatgtcaatg gcacgatagg cggaaaatgt ataaataatc gaaaataaca
      661 gcgaaatata acgattgttt tttatttgtt tcatagaaaa ataagaaacg accattgtta
      721 aaaaagtgat aaaaaaatag tagatattat aacttaaaac aagatttccc atcattttat
      781 aaaataaaaa agctggataa agagtaagcc atggataaaa aatattcatc attgttccat
      841 gatgatcgaa gttattgaag tttactggag aactccatac attatctagt cctatgattc
      901 gagataaatg aaagtaagta tcctgattga cactatgata gattccctta gacatgaata
      961 gagggatgac gtaagcaaaa cttagaaata acataaaaat gatatctatt atttggtttt
     1021 t
//
LOCUS       DAGCXG010000242         1012 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.252, whole genome shotgun sequence.
ACCESSION   DAGCXG010000242 DAGCXG010000000
VERSION     DAGCXG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.252"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            46..>1012
                     /locus_tag="MB022_002847"
     CDS             46..>1012
                     /locus_tag="MB022_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4467772.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTETDQLQNQVLLDNFVEIMK
                     EDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAEELEKTVVVYEELESYGI
                     DADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEKAIAAKL"
BASE COUNT          429 a          149 c          160 g          274 t
ORIGIN      
        1 aaaatcctga atatatacaa gtttctaaga agaaggagaa atttaatgtc tacagaaaat
       61 caatctcaaa aaatgataat taaaaattgg ggatataaag caacgaatgt ttatattgaa
      121 aaatattata aatttcctag ttttttaatt cagaattcta aatactctaa aatgaaagac
      181 agctcaaaaa tagcctatat gctttttaaa aatgaatttc gtagagcctt acatgaaaaa
      241 tggatcgacg atgatggtct attatatttg gaatttacac aagcagacct aatggacaaa
      301 ttaaattgct accaaggcaa agtaaaaacc attttaaacg agttagaaac atataatttg
      361 atttcagtcg taaaaggaga atttaaccca aaaacgaaaa aaaatgaaaa aaataaatac
      421 tatttacttc aacctgaaat aagtgccgat gatcttttta ttcaaaattc tttcgatgac
      481 gatttaagcg aaactattga taaatcaacc tcttctcgga atgcggaaat cgcacgtcga
      541 gaaaaaacag ctgaaaccct tgataacact ggaaatgcgg aaatcgcaca agattattat
      601 attaataaat ctttagacac taaaagacac ttaaaagaca ctgaaacaga ccaattacaa
      661 aatcaagttt tgctagataa cttcgttgaa atcatgaaag aagacagcat aaacacattt
      721 gtaccagaaa aggtattaag cttgataaaa acattttcta acacttacga agaagcccaa
      781 caaaccgtca ccacaataca caacgcaaaa cataaagccg aggaacttga gaagacggta
      841 gttgtgtatg aagaacttga atcttacgga attgatgcgg ataaaggatt atatatgacg
      901 ttgctgaaag cttatcaaaa acaaaagact gagaaagtaa ataatttgca gaatttgatt
      961 tttgtgtatg taaaaaactg gtttattgaa aaagctattg cggcaaagct tg
//
LOCUS       DAGCXG010000243         1000 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.253, whole genome shotgun sequence.
ACCESSION   DAGCXG010000243 DAGCXG010000000
VERSION     DAGCXG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.253"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            369..1000
                     /locus_tag="MB022_002848"
                     /pseudo
     CDS             369..1000
                     /locus_tag="MB022_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAGCXG010000244          956 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.254, whole genome shotgun sequence.
ACCESSION   DAGCXG010000244 DAGCXG010000000
VERSION     DAGCXG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.254"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(38..952)
                     /locus_tag="MB022_002849"
     CDS             complement(38..952)
                     /locus_tag="MB022_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467773.1"
                     /translation="MLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLF
                     FSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESF
                     ENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEY
                     INDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKN
                     LSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILL
                     TIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          308 a          162 c          145 g          341 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttt
//
LOCUS       DAGCXG010000245          943 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.255, whole genome shotgun sequence.
ACCESSION   DAGCXG010000245 DAGCXG010000000
VERSION     DAGCXG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.255"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>943
                     /locus_tag="MB022_002850"
     rRNA            <1..>943
                     /locus_tag="MB022_002850"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAGCXG010000246          934 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.256, whole genome shotgun sequence.
ACCESSION   DAGCXG010000246 DAGCXG010000000
VERSION     DAGCXG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.256"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            175..561
                     /locus_tag="MB022_002851"
     CDS             175..561
                     /locus_tag="MB022_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4467774.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            659..850
                     /locus_tag="MB022_002852"
     CDS             659..850
                     /locus_tag="MB022_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467775.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          353 a          145 c          141 g          295 t
ORIGIN      
        1 taaagataac tatccattat caactaaaaa aacatttttg aaaataaaca tcagcagtcc
       61 tatcaatgca tgtgagctta tagaagttat tcgcagcgca gcataattgc attagcttct
      121 tttcgtgttt tttgcctctt ttccctttaa atcttaatgg aggaggtgtg caatatgtca
      181 gataacgacg aacgcgtaaa tgcccagttg attcgattct ttgaaaaggt cattttgaac
      241 accgctaata cttattataa aaaacaagaa gaaattcaaa aacatgaaca acttgatcaa
      301 ctcccaccat atttgtttac taaagatact ataaaattta aacaaccgat taatgtttta
      361 aatgtgactc tccttgttga agatataaaa ttagcaaaaa tccttccttt gttgaaagaa
      421 cctgaacaaa tttttcttat cgaaaaattt atctttaata aaactgataa agaaattgga
      481 aaggttctgg gtattacaag acagggtgcg acaaatttaa aacatcgact ttacaaaaaa
      541 attcaaaaaa tgctggttta atatctgcat aataactaat tttgtatgct tctttgctcg
      601 tcctcctcct tcccaaaaaa ataaatagat tataagttca tgggaagaag gtaaatttat
      661 gaatacttta aaagaagatt tcattttagc aaagaatgga aatgaagaag cagttgtggc
      721 cattctcaaa agatttagcg ctttggtcca taagcaatcc tggagaactg gaaaatatga
      781 tcaagattgt tatcaagaat gtatgcttgc aatatatcta gctatctcga aattcgaaat
      841 taaagaataa ctcaactaag ggagtctgtg acataagtaa aaagatagtt tcaaatttcg
      901 aacaataaat ttgaagccat cttctttata atta
//
LOCUS       DAGCXG010000247          885 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.257, whole genome shotgun sequence.
ACCESSION   DAGCXG010000247 DAGCXG010000000
VERSION     DAGCXG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.257"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            71..>885
                     /locus_tag="MB022_002853"
     CDS             71..>885
                     /locus_tag="MB022_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467776.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGILS"
BASE COUNT          278 a          119 c          125 g          363 t
ORIGIN      
        1 tttttctgta aaaatattta tagtttatgc atttgttagt ttattatatt tatttccagc
       61 atttaaaaac atggaaatat ttggctttta tgatacggca tttcacatta atcgtgcgtt
      121 aagtttagaa tctattttta gctcccctat taattttgaa acgtttagat catatggcat
      181 gcaagtaaat aatttttacc catggttaac tctatatcct ttatttttat tgatcaagtt
      241 tactaattta gcaataggct ataacctttt tctttatatt gttacgctca ttactctatt
      301 tatatgtcat tatgtaatgt atgaaattac caaaaaacat gttactagct cctttttcgc
      361 tattatatat acaacatctt cttttaggag tgtagaaata tttcttcgag gggcaatggg
      421 ggaattactc gcaatgtcaa tacttcctct tattttactc gggtttataa agctttacga
      481 ctcaaaaaaa gaatcttggg ttatgttggc gattagcatg actttattaa tttacactca
      541 tgttctttct gtagctatga ctatggtaat gattataatt gctttaatta ttcaatttta
      601 tcggaaaaag aaaatagaaa tcttcttaat tattaaatta ataaaagctg ctatagttac
      661 tttacttctt tcactagcgt ttataggtcc tatgattgag cagacattgt atcaagatct
      721 gaatagacct tatgtggata tattacaaaa aagagcaatt tatctgggta aagagttttt
      781 gattggaagt atagattcag aattactatc atttggtatt ggcttaactt tgcttatttg
      841 tttaattata ctaacattta actttaaaaa attaggaatc ttatc
//
LOCUS       DAGCXG010000248          864 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.258, whole genome shotgun sequence.
ACCESSION   DAGCXG010000248 DAGCXG010000000
VERSION     DAGCXG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.258"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(51..123)
                     /locus_tag="MB022_002854"
     tRNA            complement(51..123)
                     /locus_tag="MB022_002854"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:His,seq:gtg)
     gene            complement(126..199)
                     /locus_tag="MB022_002855"
     tRNA            complement(126..199)
                     /locus_tag="MB022_002855"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Trp,seq:cca)
     gene            complement(206..286)
                     /locus_tag="MB022_002856"
     tRNA            complement(206..286)
                     /locus_tag="MB022_002856"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Tyr,seq:gta)
     gene            complement(288..363)
                     /locus_tag="MB022_002857"
     tRNA            complement(288..363)
                     /locus_tag="MB022_002857"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(328..330),aa:Phe,seq:gaa)
     gene            complement(399..471)
                     /locus_tag="MB022_002858"
     tRNA            complement(399..471)
                     /locus_tag="MB022_002858"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Asp,seq:gtc)
     gene            complement(481..553)
                     /locus_tag="MB022_002859"
     tRNA            complement(481..553)
                     /locus_tag="MB022_002859"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Val,seq:tac)
     gene            complement(569..640)
                     /locus_tag="MB022_002860"
     tRNA            complement(569..640)
                     /locus_tag="MB022_002860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(605..607),aa:Glu,seq:ttc)
     gene            complement(655..744)
                     /locus_tag="MB022_002861"
     tRNA            complement(655..744)
                     /locus_tag="MB022_002861"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(708..710),aa:Ser,seq:gga)
     gene            complement(784..857)
                     /locus_tag="MB022_002862"
     tRNA            complement(784..857)
                     /locus_tag="MB022_002862"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(821..823),aa:Asn,seq:gtt)
BASE COUNT          241 a          238 c          196 g          189 t
ORIGIN      
        1 gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaaag ggcgaatgat
       61 gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact tcgccacatt
      121 cgccatggca ggggcagcag gaattgaacc cacactaacg gttttggaga ccgctgttct
      181 acctttaaac tatgccccta taaaatggag gagagtggat tcgaaccact gaaccctaag
      241 gaacggattt acagtccgtc gcgtttagcc acttcgctac tcctccatgg tggcgcggga
      301 cagaatcgaa ctgccgacac acggagcttc aatccgttgc tctaccaact gagctaccgc
      361 gccatatttt aaaattatta attgaataac aagaacgacg gttccgacgg gaatcgaacc
      421 cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac cagttcatta
      481 tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc tctcccagct
      541 gagctaaacc tccaacattc aattaaaatg acccgtacgg gattcgaacc cgtgttaccg
      601 ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aggtaaattt gctacggaga
      661 gtaagggatt cgaacccttg agacagtttg gcaccatcta catgatttcc aatcatgctc
      721 cttcggcctc tcggacaact ctccagagtg aaagaagctc aatactcttt ctcaaaatga
      781 gaactccggc agtaggactc gaacctacga catcatgatt aacagtcatg cgctactacc
      841 aactgagcta tgccggaata acat
//
LOCUS       DAGCXG010000249          854 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.259, whole genome shotgun sequence.
ACCESSION   DAGCXG010000249 DAGCXG010000000
VERSION     DAGCXG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.259"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(101..301)
                     /locus_tag="MB022_002863"
     CDS             complement(101..301)
                     /locus_tag="MB022_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4467777.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            697..846
                     /locus_tag="MB022_002864"
     CDS             697..846
                     /locus_tag="MB022_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467778.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          279 a          146 c          132 g          297 t
ORIGIN      
        1 gttttataca ctactattca gcccattcaa cgaccaatga attttttcgt acaaaaaaac
       61 cgtcttttct ttcgaaaaga cggttaaaag tcaattttta ttaagcttta acaatgtttg
      121 ttgcttgagg gccacgttga ccatcttcaa catcgtaaga cactgcttgg ccttcttcta
      181 aagttttgaa gccgtcacct tggatagctg aaaaatgtgc aaatacatca tttccatctt
      241 caccagtgat aaagccaaaa cctttgtctg cgttgaacca ttttactgta ccgttattca
      301 taaataaatt cctcctgata cgtatcaatg tacgtttttt cgttgcaata aatatttgat
      361 aggcaaaagg agttttatct atgtagatat atcgctcaaa ttacgtttca aaatagatta
      421 ctcatttaat atatcatgct ttttctaata acacaaggat aatgcaatga aatataaaat
      481 tcattgtctt ctattgtctt cgaatcgaag aaaaagttga ataaccgcga ttgattcact
      541 ttttgttttc ttgtttattt gcttttatct gcaacttttg actttaaaat ttcttaacta
      601 tgaaaacctt tcaattggtg gtatacttat aaatggaatt tcgttatcaa cgcgttcgtt
      661 gcctttcagc aagtatttaa agaaaggaga atggctatga cattctataa caaagatata
      721 caatatcgag taaccttgga tacagatttg aatctatttg tcgtttttga caaagaaaat
      781 accaatcatg tagcgacagg tgttacgatc gaacaagcag tgcaagaatt aaaaaaatct
      841 gcataaggag tgga
//
LOCUS       DAGCXG010000250          839 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.260, whole genome shotgun sequence.
ACCESSION   DAGCXG010000250 DAGCXG010000000
VERSION     DAGCXG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.260"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..340)
                     /locus_tag="MB022_002865"
     CDS             complement(<1..340)
                     /locus_tag="MB022_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467779.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
BASE COUNT          289 a          132 c          154 g          264 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      481 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      541 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      601 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      661 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      721 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      781 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaa
//
LOCUS       DAGCXG010000251          808 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.261, whole genome shotgun sequence.
ACCESSION   DAGCXG010000251 DAGCXG010000000
VERSION     DAGCXG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.261"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            18..689
                     /locus_tag="MB022_002866"
     CDS             18..689
                     /locus_tag="MB022_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBT4467780.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
BASE COUNT          304 a           86 c          181 g          237 t
ORIGIN      
        1 ttgggagggt aggtgcagtg aaggaaagat atggtcacct tactgttgtt gaggagatta
       61 ctcataatgg atataaaaaa tatcgttacc tatgtgactg tgggaatgaa acgattgtat
      121 atcgatcaaa cttactatca ggtagaacca aaagttgtgg atgtggaaat tataaaaata
      181 ggcataaata taaaaatttg acaaataaaa cttttggtaa gttagttgca atcaagccaa
      241 ctaaactgcg aagtgaagga actgttgttt gggaatgtca atgtctgtgt gggagaaaaa
      301 agttagtatc ggcaaagcga cttctaaaag gtgatgtaaa aagctgtgga tgtttgaaat
      361 caaaaaagca tcacattgaa aataaaaaat tcggattact tactgcaaag tatcctttgg
      421 acaataactg ttcatggaaa acaaaatggg tttgtcaatg tgagtgtggc agtatgtgtg
      481 agataagtta tagtaactta ttttttggac atactcaaag ttgtggatgt ttaaaaaggc
      541 aagaaacaag gacatgtgtt gaagggacag taatagagtg tctaacgtca aaattaccga
      601 aaaataatac aagtggtgtt aagggagttt atcgttctaa agagaaatgg attgcgtata
      661 taactttaaa aaaaagaggt actatttagg atcgtatgat tctattaaga gtgcagctca
      721 agttagaaaa gatgctgaaa ataaattgtt tcaacctttg attgacaaat atcgaggtaa
      781 tcattaatta ttgtggataa taattaac
//
LOCUS       DAGCXG010000252          799 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.262, whole genome shotgun sequence.
ACCESSION   DAGCXG010000252 DAGCXG010000000
VERSION     DAGCXG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.262"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            376..774
                     /gene="spxA"
                     /locus_tag="MB022_002867"
     CDS             376..774
                     /gene="spxA"
                     /locus_tag="MB022_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4467781.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          301 a          109 c          141 g          248 t
ORIGIN      
        1 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
       61 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
      121 tacagtttta tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa
      181 tttttaaatg ataaaaaaag cgagaatgct ggttttactg tatattaacc ttagataact
      241 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa
      301 tagaaacaga tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa
      361 aaggagtggt gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag
      421 gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa
      481 ccattgaaca gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa
      541 atcatctcga cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg
      601 ttacaagaat tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg
      661 attgatgata aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg
      721 cgcgatgtac gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat
      781 aatacgaaac ttggaaaag
//
LOCUS       DAGCXG010000253          795 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.263, whole genome shotgun sequence.
ACCESSION   DAGCXG010000253 DAGCXG010000000
VERSION     DAGCXG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.263"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>795
                     /locus_tag="MB022_002868"
     CDS             <1..>795
                     /locus_tag="MB022_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4467782.1"
                     /translation="ESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKL
                     KDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKN
                     SIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSD
                     FSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFV
                     EKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRER"
BASE COUNT          345 a           62 c          135 g          253 t
ORIGIN      
        1 gaaagttcta attcttcaat atcaataaca aaaaaagatg gtaaatattt taatgttaat
       61 caatttaaag atattgaaaa attaaaagaa attgaagaaa aaataattca atatgatgga
      121 ttagcaaaat taaaagatgc taaagtagtt agcggagaac aaagaataaa tagagaagat
      181 ttatctgacg aatttaagaa tgttgtttca ctcgaagcta caaataatac taaaagaaat
      241 attttattta gtagtggagt atttacaatt aaagaaggta aaaatataga agaaaatgat
      301 aagaattcaa ttattgttca tgaagaattt gctaaacaaa acaatctaaa attaggtgat
      361 gaagttgatc ttgaattact agatattgaa aaaagtggaa aaataaaaag tcataaattt
      421 aaaattatag ggatcttttc tggtaaaaaa caggaaacat atacaggatt atcttctgat
      481 tttagtgaaa atatggtgtt tgtagattat tcaacaagcc aagagatatt aaataattca
      541 gaaaataatg aaattgcaaa taaaatttta atgtattctg gtagtgcaga atctacagac
      601 ttagccttaa acaaattgaa agagcttaaa attgatgagt caaagtattt tgttgaaaaa
      661 gattctaatg cgtttgaaga gtctttagag tcagtgagtg gaataaaata tataataaaa
      721 gtaatgactt attctattat gttaggtggg atggttgttc tttcattaat cttgattcta
      781 tggttaaggg aaaga
//
LOCUS       DAGCXG010000254          758 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.264, whole genome shotgun sequence.
ACCESSION   DAGCXG010000254 DAGCXG010000000
VERSION     DAGCXG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.264"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(324..518)
                     /locus_tag="MB022_002869"
     CDS             complement(324..518)
                     /locus_tag="MB022_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467783.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(618..704)
                     /locus_tag="MB022_002870"
                     /pseudo
     CDS             complement(618..704)
                     /locus_tag="MB022_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          262 a          131 c          116 g          249 t
ORIGIN      
        1 cattagacca attacctgat aaacctccag ttttttgctc ttacttaaat ggatcaatgg
       61 caatttcatc acaaacctat agaggatgag tgacttctgc ctaattacgt taaaaaatat
      121 tgaaagtaac tctatgtagc aaaaaatatc tagaatttca tttcctcaaa actgatcgat
      181 attgtatcta aaaatatata ctacctaaaa taggtagtat atattaaaat atgtgctttt
      241 gttagaaaaa tcaatacata aattatcata tctgtatatg tcatttaggt atttcagtta
      301 tgttataata gttttgctgt ctgctacccc ggcctaatct ggcaagggga ggaggagata
      361 taatgaaata cctttttgat tttgtttttg caatcaagcc acttgcagat gtagtgtgca
      421 gttacctctg caaatggctt gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt
      481 aattctataa tatagaaacg taaaaaaaac cccatcagcc tgcacgctga tggggtttgg
      541 tgatataatg aaatacctct ttgttactca taattgtacc ataattagtc acgaatgcaa
      601 ccaatagaaa aaactactta gaacggtaga tcatcgctaa taacaatgca ttgtccacca
      661 aatggatttt gtgaagcatt aaatacattg ttattaattg tcgctgcttt taactaggcc
      721 tctccccttc tattataaaa gaagatatat atcatttc
//
LOCUS       DAGCXG010000255          755 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.265, whole genome shotgun sequence.
ACCESSION   DAGCXG010000255 DAGCXG010000000
VERSION     DAGCXG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.265"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..104
                     /locus_tag="MB022_002871"
     CDS             <1..104
                     /locus_tag="MB022_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBT4467784.1"
                     /translation="ETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..721
                     /locus_tag="MB022_002872"
     CDS             104..721
                     /locus_tag="MB022_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467785.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          283 a           95 c          162 g          215 t
ORIGIN      
        1 ttgaaacagt agaatttcca attgaagaag gagctttgat tattgcaact gttgatgatc
       61 caaaacagaa tgttttcact cttgagaaag ttggggtgat ttgatggtta aagtaaagag
      121 aatgatggag actgatgaat ctggtgttaa acgtcaattt atgccaatta cacatgtttc
      181 tgcagtttta ggcttagaaa aaatgatatc aggacaatca aaagttcttt cggttaatgg
      241 taaatatggt gcagtcattt taacgaaagc tgatttagga ttagagaatg caattactga
      301 actaccttat gcaagcgaga caagcgatgg tattcttact gctgaaatgt ttcaaaagat
      361 cgtaaatgga gaaggtggaa catatatttt gccgattgct actcctgaac aattaggagg
      421 tataaaagtt ggggaactct tagaagttac tgaagaaggt gttttatctg caactaagca
      481 aacagacttc aatttctctg aagaattaaa gggaaaatta gaatctctaa aaatattaaa
      541 agcaggtgca aatatatcaa ttgcagaaga tggaacaatt agttctacag gtggtagtgg
      601 gactggtggt gttaatcagt catatgtaga tcaaaaattc caagaagctg taaatcaagc
      661 agaaaattac acaaacgaaa gaattccaaa ttttactttt gaaaaaatcg gggaggtata
      721 gaaaatgaca gatatcgtaa aaataaaaca aagcg
//
LOCUS       DAGCXG010000256          754 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.266, whole genome shotgun sequence.
ACCESSION   DAGCXG010000256 DAGCXG010000000
VERSION     DAGCXG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.266"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>754)
                     /locus_tag="MB022_002873"
     CDS             complement(<1..>754)
                     /locus_tag="MB022_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBT4467786.1"
                     /translation="TSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFY
                     ITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFG
                     TNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKA
                     ALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSS
                     GIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFL"
BASE COUNT          286 a          127 c           91 g          250 t
ORIGIN      
        1 aagaaacgca taattcactt tacttctaaa aaatgttttg agtaaactaa agatatttaa
       61 cacatagaaa gcatagatac atataaaagc aattatacca cttgatacaa gtacttgaag
      121 aaatccatta tgcatgtttc catgtgctct ttttagttca ttcacatctg tagtatcaag
      181 atctactttt ttcaaaaatt ttgcatcgct attcccatag aaatcggtct ctccataacc
      241 aaataacaaa gttggtctta taactttaag cgcagctttc caaatcgtaa aacgaccagc
      301 tgaaatttcg gagttatcac tagaatgctg taatattact tctttttctg gaagtggagt
      361 tttatctgat tcatttgact ctataacttc tacttttcta tcaatcgata caggaattgt
      421 tgctaaaaaa tccttagcat aattagtacc aaaagaaaaa tataccaata gaactaataa
      481 tatgcttacc atccatttga tttttacagg tagtacttct ctaatgctct ttctaaattt
      541 tgaattggca aacaacaaaa gaaataatac taaaaaagaa caaattacta cctgggcacc
      601 tctagacgaa gaaagtgtaa tataaaatat ttgtacgata aaattcatca tataaaaaac
      661 acgaatcgtt tttgttattt ttgaaattaa tacactaatt aaacttaaaa taatagaagt
      721 atatccaaac atagctccaa catttggact tgta
//
LOCUS       DAGCXG010000257          728 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.268, whole genome shotgun sequence.
ACCESSION   DAGCXG010000257 DAGCXG010000000
VERSION     DAGCXG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.268"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>728
                     /locus_tag="MB022_002874"
     CDS             <1..>728
                     /locus_tag="MB022_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4467787.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLD"
BASE COUNT          258 a          137 c          132 g          201 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcattgcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc aatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      421 taacattttt tgaataccta aaagaacgcg gtttacaagg tacagaactc gttatttctg
      481 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgca agttggcaaa
      541 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      601 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gctcgtgagg
      661 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
      721 cattggat
//
LOCUS       DAGCXG010000258          717 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.269, whole genome shotgun sequence.
ACCESSION   DAGCXG010000258 DAGCXG010000000
VERSION     DAGCXG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.269"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>717
                     /locus_tag="MB022_002875"
     CDS             <1..>717
                     /locus_tag="MB022_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467788.1"
                     /translation="NDYVSSMITGQGSAGGYVSLWPKEKPSNIFIMDNPDLEKAREVL
                     RMNKNGIAFSKKGWRGPFTSAWTLDSIFNANFIQTGLIKADIFQNSFNKTGDVLKLVN
                     GLLQIWNNKKKIMELTKKGMEFWNSKGSIGTIGTTDSAGNPFPGASTPTPIEDNSLVI
                     RTNGDGKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGG
                     ESTGGYPSELKTDAEKRAWR"
BASE COUNT          260 a           91 c          154 g          212 t
ORIGIN      
        1 taatgattat gtttcttcaa tgattacagg tcaaggcagt gcaggaggat atgtaagttt
       61 atggccaaaa gaaaaacctt ccaacatctt tattatggat aatccagatt tagaaaaggc
      121 aagagaagta ctaagaatga ataaaaatgg gattgctttt tctaagaaag gatggcgggg
      181 gccttttact tcagcttgga cattagatag tatatttaat gccaatttta tacaaactgg
      241 attaatcaaa gcagatatct ttcaaaattc ttttaacaag actggagatg tattgaaatt
      301 agttaatgga ctactccaaa tttggaacaa caaaaagaaa ataatggagt taactaaaaa
      361 aggaatggaa ttttggaatt ctaagggttc aattggaacg attggaacaa ctgattctgc
      421 tggtaatcct tttcctgggg cttctactcc aacccctatt gaagataatt ctttagttat
      481 tcgtacaaat ggagacggca aatatatttt gatttctcct aaagtaggaa aaggattagt
      541 tttattagga aatggtaaag caatttattt tggagactta gatgtacaag gaaaacttac
      601 agttaaagga aaagaaataa ttcctggaca aggtggtggt ccatcgggag gtggagaatc
      661 tactggtggg tatcctagtg aattaaaaac agatgcagaa aagagagctt ggagaat
//
LOCUS       DAGCXG010000259          705 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.270, whole genome shotgun sequence.
ACCESSION   DAGCXG010000259 DAGCXG010000000
VERSION     DAGCXG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.270"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..258
                     /locus_tag="MB022_002876"
     CDS             <1..258
                     /locus_tag="MB022_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467789.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="MB022_002877"
     CDS             260..397
                     /locus_tag="MB022_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBT4467790.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..>705
                     /locus_tag="MB022_002878"
     CDS             434..>705
                     /locus_tag="MB022_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467791.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          279 a           98 c          138 g          190 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa acttt
//
LOCUS       DAGCXG010000260          705 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.271, whole genome shotgun sequence.
ACCESSION   DAGCXG010000260 DAGCXG010000000
VERSION     DAGCXG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.271"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..380
                     /locus_tag="MB022_002879"
     CDS             <1..380
                     /locus_tag="MB022_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467792.1"
                     /translation="EDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIE
                     DTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVV
                     KFNGTEIGRQIFDIKVNKPVEKN"
     gene            394..>705
                     /locus_tag="MB022_002880"
     CDS             394..>705
                     /locus_tag="MB022_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467793.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FS"
BASE COUNT          260 a           97 c          128 g          220 t
ORIGIN      
        1 cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta attaatccag
       61 ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta acagctgtgg
      121 tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt ttaaacaaga
      181 atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct caagttaacg
      241 atataaactt taatataaac gccagaaatg tcttggttga agaagcagga gagcattatg
      301 ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc aaggtcaaca
      361 aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc atcaaataca
      421 catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc ggtaattcta
      481 gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga taatcaactt
      541 gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa aatttttgat
      601 gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga tgaagattta
      661 aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcc
//
LOCUS       DAGCXG010000261          664 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.272, whole genome shotgun sequence.
ACCESSION   DAGCXG010000261 DAGCXG010000000
VERSION     DAGCXG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.272"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..190
                     /locus_tag="MB022_002881"
     CDS             <1..190
                     /locus_tag="MB022_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467794.1"
                     /translation="WVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGILIS
                     AIITQLFLVFGEKKRSKK"
     gene            complement(281..646)
                     /locus_tag="MB022_002882"
     CDS             complement(281..646)
                     /locus_tag="MB022_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467795.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          250 a           94 c          118 g          202 t
ORIGIN      
        1 ttgggtatac tcacaaacta ttttttatgg aggaattgtt ttaattagtt tactttcttc
       61 aacactttat tctttgaata ttatagatgt ttccacctca aattcaatat ctattattgg
      121 aatccttatt tctgcaatta ttacacaact gtttttagtg tttggagaga agaaaagaag
      181 taaaaaatag aaaaactagt gatagttttg gtgatataag aaaaacatat atgtataaaa
      241 agaagaaagt agcttctctt tgtacatata tggtttgttt ttatggatgc cgagcttttt
      301 tataagataa ggctaccgcc cataacgacc caaggatttg tggaactaag taaatacctg
      361 caagaagcaa gatcagctgg gtcggtttta aaggatgata gaagtaacgc cattcagtaa
      421 aattggcgca aaataaatag gaagtgacag tagatataat attggcaatg aataaagata
      481 taatacgcct gttcctttga aaataatatc cagcaaaaag agataaaaat atcaatgtga
      541 tgaacccgct tacggaatga taacgaaaat caagcagtaa ggcgaaatgc acataaggaa
      601 caaaagataa aagcaacgga aggtttttcc agcaagtttt tatcatcttt catcaccacc
      661 taac
//
LOCUS       DAGCXG010000262          658 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.274, whole genome shotgun sequence.
ACCESSION   DAGCXG010000262 DAGCXG010000000
VERSION     DAGCXG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.274"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(549..>658)
                     /locus_tag="MB022_002883"
     CDS             complement(549..>658)
                     /locus_tag="MB022_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4467796.1"
                     /translation="YICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          224 a          109 c          104 g          221 t
ORIGIN      
        1 agtgatgaaa tgggtggaaa ttgattctta tagacagttt gtctcataac attgaagtat
       61 tttgcaattt tagcattgaa acactttttt aatcttcaac gttgttctta taaacaagat
      121 gattgggaat cttttgaatt aacactgtat agcttagaac accctttata gaaaacattc
      181 gcttagcaat cttaataatt ccttgtgccc gatgtcgttt agattcagta ggttcctgct
      241 catcgatcat ggaaaaaatt tgaaggatca gatccttaat aaatttattt aagagaggat
      301 gatccattgc ttcatccata ctcgataaac tagtgataat gagctacacg tttttctttc
      361 aagttgatca atagcttcca cgataaaaat atctttagat aaaatcaaga accgtaggaa
      421 ctacggggat atttggtcaa taagagccac cgctattagt aaaaaataag ctatcaagta
      481 tgctctcatc ccaagaattg cctacttcaa tcgttaaaaa ccgttaggtt tagctaagtg
      541 ggggtagttc actcaatact tttatcaata atagagcgca aaactttttc tcttaattca
      601 actgaatcaa tattattgtt ttgttcataa ccatgaacta atagatcaca aatataaa
//
LOCUS       DAGCXG010000263          628 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.275, whole genome shotgun sequence.
ACCESSION   DAGCXG010000263 DAGCXG010000000
VERSION     DAGCXG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.275"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..169
                     /locus_tag="MB022_002884"
     CDS             <1..169
                     /locus_tag="MB022_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBT4467797.1"
                     /translation="NKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYH
                     ANEVLKIAEEK"
     gene            170..>628
                     /locus_tag="MB022_002885"
     CDS             170..>628
                     /locus_tag="MB022_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBT4467798.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNM"
BASE COUNT          240 a           74 c          116 g          198 t
ORIGIN      
        1 taataaaata agttttgaag aaaacattgt taaagaaatg cctgaatctg ctaatgatat
       61 gtatgagaag gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa
      121 tgatttatat catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt
      181 atttactatt tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt
      241 ggcaggaaag cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca
      301 tcctgaacat aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac
      361 gactaagtta aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac
      421 tgtagggaaa attgaagtga tacgtgattc attcttacaa ctagaaagag aagataatat
      481 atttgattta attatagatt tagatttgac ttcaccttta cgtgaagttt ctaatcttga
      541 agaattaata gatgaacatc taaaaaattt agataaagac gtctactttt cagttgtttc
      601 tgcaagacgt aatccctatt ttaatatg
//
LOCUS       DAGCXG010000264          607 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.276, whole genome shotgun sequence.
ACCESSION   DAGCXG010000264 DAGCXG010000000
VERSION     DAGCXG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.276"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(148..447)
                     /locus_tag="MB022_002886"
     CDS             complement(148..447)
                     /locus_tag="MB022_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4467799.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          200 a          110 c           70 g          227 t
ORIGIN      
        1 gttaattaaa ggataaaata caatataaag aaacatctgt ttctttattt agaataaaaa
       61 aatacagact ctgttcaaat aaatgaacct agcctgcatt atgagattga ctatactttt
      121 taattcattg agatgaataa ctcgttttta taaatgccca aaagtaatcg gtgccgaagc
      181 cattcctcga cctaattctt tttcaaattt tcctattgtt gatatctcca tataaaacgg
      241 tgatactggt ttagacaatt ctcccctgat tgctaacgga cgtaaactac gttccaaatt
      301 atttatgact ttaggataat actgaccttt ttctaagcct gtctttgcgt ctatcaaaag
      361 tatacgttcc ttttctttga tctctggatt taaaacgaga tcatataatt ttagtaccat
      421 gttttctatt tcttcacttt tagacatcac gatttcctcc caaaaatata tttcttccca
      481 ctacattcta taaacaaagt atacgctaca tctagatttt tttaaatcat tactggattg
      541 ttaaaacctt tcaatacact gttatggcaa cgatttttat tttaaaaaca ttctaaatta
      601 aatcagt
//
LOCUS       DAGCXG010000265          601 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.277, whole genome shotgun sequence.
ACCESSION   DAGCXG010000265 DAGCXG010000000
VERSION     DAGCXG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.277"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>601
                     /locus_tag="MB022_002887"
     CDS             <1..>601
                     /locus_tag="MB022_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBT4467800.1"
                     /translation="YEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGW
                     KKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAG
                     KTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVE
                     KEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVS"
BASE COUNT          242 a          102 c          138 g          119 t
ORIGIN      
        1 tttatgaaca agtgttaaaa acaacggtgg ccatgtttgc ggaaaagtat acctatgaag
       61 aaacaacgat cttgacgcaa gttcagcagc ttcagctaaa gacagtcgga aaagtaccag
      121 tagatctagg ttggaaaaag ctgtttggca aagagagcga cagaaaagaa aaggaagaag
      181 aaccgctact tcctaaagtg acaaaaggag aagcggtgac ggtagatcta caagtccttg
      241 aaaaagaaac gaagccacca caaccttata ccgaaggtac gttgatcaca gcgatgaaaa
      301 cagctggaaa aaccgtggat agcgaagaag cacaatccat attgaaagaa gtagaaggaa
      361 tcggtacaga agcaacacga gcaaatatta ttgaaacttt gaaacaaaag gaatatatca
      421 aggtagaaaa aaataagctt gtggtgacaa ataaagggat tttactttgc caagccgtgg
      481 aaaaagagcc gctgctaaca agtgccgaaa tgaccgccaa atgggaaagt tacttattaa
      541 agattggtga gcagaaagga acacaagcta ctttcttagc taatatccaa aaatttgtct
      601 c
//
LOCUS       DAGCXG010000266          591 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.278, whole genome shotgun sequence.
ACCESSION   DAGCXG010000266 DAGCXG010000000
VERSION     DAGCXG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.278"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(152..225)
                     /locus_tag="MB022_002888"
     tRNA            complement(152..225)
                     /locus_tag="MB022_002888"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:Arg,seq:cct)
     gene            379..543
                     /locus_tag="MB022_002889"
     CDS             379..543
                     /locus_tag="MB022_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467801.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
BASE COUNT          242 a           97 c          102 g          150 t
ORIGIN      
        1 ttcatattgt tcgagagctt gaagatcagg ataaagaaaa aatcaatact gtgttcgata
       61 gtttatataa aaatgataac tagtttggca catttttggc acaaatacaa aacaaacccc
      121 tgtaccgcaa aggatacagg ggttatttat acgttcctga ctggaattga accagcgacc
      181 tatcgcttag gaggcgattg ctctatccta ctgagctaca ggaacaattt caataaatca
      241 attgtagctt gtttgttttt tacttgcaag cttatctgct aaaaaaatca gcttataagt
      301 taggcaaaac gtttccacta gtccttaaca gatgataaac tagaggtaga atgaaataat
      361 gtagggaggt cctttccaat gaatgataag ccaaaagaaa aagtatacgc agaacgctta
      421 gattcatcta aaaaaaatcg ggaaaagatt acgaaagaag caatccaaga ccacaaaaaa
      481 gaaaacccta aaacaaaaga aaacaaaaaa ttcaaattta cgaaaaaaga ggatttactt
      541 taacattttc cttcaaatgc caaatgaaaa acaaaagggt ggtgacaaaa a
//
LOCUS       DAGCXG010000267          580 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.279, whole genome shotgun sequence.
ACCESSION   DAGCXG010000267 DAGCXG010000000
VERSION     DAGCXG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.279"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            293..>580
                     /locus_tag="MB022_002890"
     CDS             293..>580
                     /locus_tag="MB022_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4467802.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNN"
BASE COUNT          241 a           93 c           90 g          156 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccaacat
       61 ccctaatgaa atagcgtgtg gaaaaaaagc tacagtagtt atgtattttg gagcaaaagg
      121 aaatgcacct tatacaatcg catacggtcc agcaacttgg agcgaataat aattcataaa
      181 taaaaacacg ccccctccct cgccaaagtt tgtggacgtg atgaaaaata aacctgctat
      241 aatcggctta cttatctatt cctattatag caacaaatag gagatgaaaa caatgtggat
      301 cgaaactaaa actgataaaa acggaaaaaa agtatataaa tataatgagc gatatattga
      361 tccaaaaact agaaaaagaa aaaaagtgag cattacttat aaaaataaat ctcgagaaac
      421 tcagaaagtg gcattgctag agttaaataa aaaaattgat ataaaattaa acgaaaaaac
      481 acttcagaag cctgatctaa catttcatga gcttgttgaa gaatggttag ttatatacaa
      541 aagacaagtt aaggaatcta catattatcc tacgaataat
//
LOCUS       DAGCXG010000268          578 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.280, whole genome shotgun sequence.
ACCESSION   DAGCXG010000268 DAGCXG010000000
VERSION     DAGCXG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.280"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            126..413
                     /locus_tag="MB022_002891"
                     /pseudo
     CDS             126..413
                     /locus_tag="MB022_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            427..>578
                     /locus_tag="MB022_002892"
     CDS             427..>578
                     /locus_tag="MB022_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467803.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT          200 a           91 c          122 g          165 t
ORIGIN      
        1 cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg
       61 taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc
      121 tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt
      181 ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa
      241 ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc
      301 gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa
      361 gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga
      421 aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg
      481 aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg
      541 agaccaagat ttgattttga taaaaaacag tgggttga
//
LOCUS       DAGCXG010000269          575 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.281, whole genome shotgun sequence.
ACCESSION   DAGCXG010000269 DAGCXG010000000
VERSION     DAGCXG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.281"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            184..540
                     /locus_tag="MB022_002893"
     CDS             184..540
                     /locus_tag="MB022_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4467804.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          226 a           83 c          111 g          155 t
ORIGIN      
        1 cgaaaaaagt accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt
       61 ccttaacttt cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt
      121 gccaacaact ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag
      181 tacatgaaaa aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt
      241 gcagcttatc attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc
      301 gaaatgtcag ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa
      361 gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat
      421 ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc
      481 tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag
      541 taaatggtct taaaatttta ttagataaaa agagg
//
LOCUS       DAGCXG010000270          575 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.282, whole genome shotgun sequence.
ACCESSION   DAGCXG010000270 DAGCXG010000000
VERSION     DAGCXG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.282"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            312..>575
                     /locus_tag="MB022_002894"
     CDS             312..>575
                     /locus_tag="MB022_002894"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4467805.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAGCXG010000271          534 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.283, whole genome shotgun sequence.
ACCESSION   DAGCXG010000271 DAGCXG010000000
VERSION     DAGCXG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.283"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>534
                     /locus_tag="MB022_002895"
     CDS             <1..>534
                     /locus_tag="MB022_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4467806.1"
                     /translation="ISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWI
                     IDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTII
                     GEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFT
                     LLEKKMEPQYTFEKDKY"
BASE COUNT          190 a           65 c           97 g          182 t
ORIGIN      
        1 aatttcttat caataccatg taggacagta tttacccgac tggcatcctt gggaagatta
       61 tcgcaatttt tttgtttcaa acaaaaaaac taatgggtgt agagaaatat tcgctattga
      121 attaccttgg attatagata catttgggaa agttaaatcg tttactgtaa tgacccagaa
      181 attatccaat cttgatattg attattcgga cagtttcact cttattattg agcatgaaac
      241 tggtatagtt ggaactttaa gtgtagatgt tgtttcaaga gtagctaaaa gaaatttaac
      301 tataattggt gaaaagattt taatagaatg ggaaggtaac ccggattctc tattttcatg
      361 gaatgaagaa acttctagta tgacagatat tcaattgtac gatgaggtta tgcatgataa
      421 taattattca catactatta ttgaagatgc atatttagag gagattaaag aatttttcac
      481 gcttttggaa aagaaaatgg aaccccaata tacttttgaa aaagataagt atat
//
LOCUS       DAGCXG010000272          526 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.284, whole genome shotgun sequence.
ACCESSION   DAGCXG010000272 DAGCXG010000000
VERSION     DAGCXG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.284"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
BASE COUNT          195 a           70 c           63 g          198 t
ORIGIN      
        1 caaatataaa atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta
       61 cgttttcctt ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt
      121 tgtttgtaat tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc
      181 agacattcta tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt
      241 atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata tatatctata
      301 gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt
      361 tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct
      421 ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt
      481 atgtgacaaa aagaactagt agaagaagga ataatggctt gataat
//
LOCUS       DAGCXG010000273          524 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.285, whole genome shotgun sequence.
ACCESSION   DAGCXG010000273 DAGCXG010000000
VERSION     DAGCXG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.285"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            165..359
                     /locus_tag="MB022_002896"
     CDS             165..359
                     /locus_tag="MB022_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467807.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          221 a           65 c           86 g          152 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat cctggaattg gagatctcgt agag
//
LOCUS       DAGCXG010000274          503 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.286, whole genome shotgun sequence.
ACCESSION   DAGCXG010000274 DAGCXG010000000
VERSION     DAGCXG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.286"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..430)
                     /locus_tag="MB022_002897"
     CDS             complement(<1..430)
                     /locus_tag="MB022_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4467808.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          143 a           98 c           71 g          191 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcatttgagg ggcggtacat aaa
//
LOCUS       DAGCXG010000275          495 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.287, whole genome shotgun sequence.
ACCESSION   DAGCXG010000275 DAGCXG010000000
VERSION     DAGCXG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.287"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(72..269)
                     /locus_tag="MB022_002898"
     CDS             complement(72..269)
                     /locus_tag="MB022_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4467809.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          160 a           90 c           72 g          173 t
ORIGIN      
        1 ttacggtgag gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt
       61 aacgttcaac attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata
      121 tcataagtaa ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag
      181 aaatgagcga atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag
      241 ttaaaccatt ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg
      301 tgttttattg caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct
      361 cagattacgt ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa
      421 ggataatcac tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac
      481 tttccattta cgtaa
//
LOCUS       DAGCXG010000276          480 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.288, whole genome shotgun sequence.
ACCESSION   DAGCXG010000276 DAGCXG010000000
VERSION     DAGCXG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.288"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>480
                     /locus_tag="MB022_002899"
     CDS             <1..>480
                     /locus_tag="MB022_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBT4467810.1"
                     /translation="HFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
                     IYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYL
                     "
BASE COUNT          166 a           80 c           95 g          139 t
ORIGIN      
        1 cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg ctactatctt
       61 cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt
      121 tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta tcaaatttgg
      181 aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt
      241 aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt
      301 tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag
      361 cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt
      421 aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg
//
LOCUS       DAGCXG010000277          463 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.289, whole genome shotgun sequence.
ACCESSION   DAGCXG010000277 DAGCXG010000000
VERSION     DAGCXG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.289"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>463
                     /locus_tag="MB022_002900"
     CDS             <1..>463
                     /locus_tag="MB022_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4467811.1"
                     /translation="GNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLK
                     AKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLAD
                     TISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTE"
BASE COUNT          187 a           55 c           87 g          134 t
ORIGIN      
        1 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
       61 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
      121 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
      181 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
      241 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
      301 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
      361 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
      421 cacaagaact aaagagttat gatgtttcgt acactactga aca
//
LOCUS       DAGCXG010000278          448 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.290, whole genome shotgun sequence.
ACCESSION   DAGCXG010000278 DAGCXG010000000
VERSION     DAGCXG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.290"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            68..280
                     /locus_tag="MB022_002901"
     CDS             68..280
                     /locus_tag="MB022_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467812.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          137 a           72 c           92 g          147 t
ORIGIN      
        1 tctaattagc ttttttagtc aatctaaagt tctttattct atgatataat gttactgtag
       61 aaagtttgtg gcggtttggc aaatccaatt gaaggagcgt ggtgcctatg ggcttattag
      121 cttatcggac gtactctgta gaaaggaaat gcctgatttg ttctcattgg atcaaatcat
      181 tgctattatc ggagcagcag cagtagttat tactgccgta gcgaaagcta gcgattcgat
      241 tttagcttgg ttaaagttca aaaaagaaca aaaaaaatag agccgtctca acttttggcg
      301 aggtagacga gctctaaagc tttatttaca tgccatccgc tttgaacgga ctacactggg
      361 catgtgacag catgcccttt catttatatt atatctcatt cttttatata atgaaagtaa
      421 ataaatcatg gaggataaaa tatggata
//
LOCUS       DAGCXG010000279          443 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.291, whole genome shotgun sequence.
ACCESSION   DAGCXG010000279 DAGCXG010000000
VERSION     DAGCXG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.291"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>443)
                     /locus_tag="MB022_002902"
     CDS             complement(<1..>443)
                     /locus_tag="MB022_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467813.1"
                     /translation="QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHI
                     IKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQV
                     NANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKI"
BASE COUNT          135 a           79 c           62 g          167 t
ORIGIN      
        1 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
       61 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
      121 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
      181 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
      241 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
      301 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
      361 ataatccatt cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc
      421 cggctgagaa attatttttt gat
//
LOCUS       DAGCXG010000280          437 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.292, whole genome shotgun sequence.
ACCESSION   DAGCXG010000280 DAGCXG010000000
VERSION     DAGCXG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.292"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            141..305
                     /locus_tag="MB022_002903"
     CDS             141..305
                     /locus_tag="MB022_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467814.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
BASE COUNT          155 a           57 c           91 g          134 t
ORIGIN      
        1 aaaaggaata tttctatgag ataacgaaaa tggcctgtat aataaagaag aggcctagaa
       61 gaacatcata caataagata gctttcaaaa tactcattga aagaaggaaa tactataacg
      121 taagaaagga gatgaataaa atgaaaaacc tgattgctgg cggctttttg tttgtgggtg
      181 gtggcatact tatgaataca gataactttg ggaatgtcat cccttttctt ggtgcagctt
      241 gtgctttgat tggtgtagga atgctgattt atttcgtatt tagcgatgat gggaagtatg
      301 gttgattatg agtagccaac tctactcttt cgtaaagatt acaacatacg tgacaaaaac
      361 agaaagcttg attcatcata ataataaaag aaaaagaaac ccaagtgatt ggatttcttt
      421 ttctttgcat aagcttt
//
LOCUS       DAGCXG010000281          392 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.293, whole genome shotgun sequence.
ACCESSION   DAGCXG010000281 DAGCXG010000000
VERSION     DAGCXG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.293"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            3..371
                     /locus_tag="MB022_002904"
     CDS             3..371
                     /locus_tag="MB022_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBT4467815.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          128 a           59 c           87 g          118 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tt
//
LOCUS       DAGCXG010000282          391 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.294, whole genome shotgun sequence.
ACCESSION   DAGCXG010000282 DAGCXG010000000
VERSION     DAGCXG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.294"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>391
                     /locus_tag="MB022_002905"
     CDS             <1..>391
                     /locus_tag="MB022_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4467816.1"
                     /translation="SFEPTDSFQYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAE
                     RTGINVFKNELNESRLKTYDTLLNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDN
                     SSYPKPISDYINYLNNMKNRYEQKNVNN"
BASE COUNT          157 a           54 c           59 g          121 t
ORIGIN      
        1 tagctttgag ccaactgatt catttcagta tcttagcaaa gatattgaaa ttgaagatat
       61 ttataaaatt ttagctcaag agctatctaa aaatgttatt agtttgccaa aggtttttta
      121 ttttccagcc gaaagaactg gtataaatgt ctttaagaac gaactaaatg aaagtcgctt
      181 aaaaacatat gataccttgc tgaatactgt gcaatttact aatttaacta accaaaaaaa
      241 gaaagctgcc ctcaagtacg atctgttaaa aaagaatatt gagctgttaa tggagacaac
      301 ggataatagt tcttatccaa agccaatatc ggattatatt aattatctga ataacatgaa
      361 aaatagatat gaacagaaaa atgtcaataa t
//
LOCUS       DAGCXG010000283          370 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.295, whole genome shotgun sequence.
ACCESSION   DAGCXG010000283 DAGCXG010000000
VERSION     DAGCXG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.295"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(44..>370)
                     /locus_tag="MB022_002906"
     CDS             complement(44..>370)
                     /locus_tag="MB022_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467817.1"
                     /translation="GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT          102 a           91 c           66 g          111 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc
//
LOCUS       DAGCXG010000284          365 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.296, whole genome shotgun sequence.
ACCESSION   DAGCXG010000284 DAGCXG010000000
VERSION     DAGCXG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.296"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>365
                     /locus_tag="MB022_002907"
     CDS             <1..>365
                     /locus_tag="MB022_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4467818.1"
                     /translation="TRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTA
                     CNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEK
                     KDASLVKEAFGRIPYSLTDV"
BASE COUNT          112 a           69 c           86 g           98 t
ORIGIN      
        1 acccgaaaat tgaagaagtg tttagcaaag cgtgggcttc aactcagtcg gcgccgaatc
       61 ggtcgaatca tgaaacgccg cggattgaca tctacctata cgatcgctca ttttaaaggg
      121 caacgaacag cttgtaatga agcgaaaaca gcgaatgtat tagatcggac ctttacacaa
      181 gaacagccat tggaagccat cgttacggat cttacttatg ttcgcgtggg gaaaaagtgg
      241 cattatatct gcttaatact tgatttgttt aatcgagaaa ttattggtta ttcctgtggt
      301 gagaagaaag atgcctcatt ggtaaaagaa gcctttggac ggataccgta ttctttaaca
      361 gacgt
//
LOCUS       DAGCXG010000285          364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.298, whole genome shotgun sequence.
ACCESSION   DAGCXG010000285 DAGCXG010000000
VERSION     DAGCXG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.298"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>364
                     /locus_tag="MB022_002908"
     CDS             <1..>364
                     /locus_tag="MB022_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBT4467819.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILST"
BASE COUNT          137 a           62 c           76 g           89 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 caca
//
LOCUS       DAGCXG010000286          360 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.299, whole genome shotgun sequence.
ACCESSION   DAGCXG010000286 DAGCXG010000000
VERSION     DAGCXG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.299"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>360
                     /locus_tag="MB022_002909"
     CDS             <1..>360
                     /locus_tag="MB022_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HBT4467820.1"
                     /translation="WVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAI
                     DADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLY
                     TKTEHRTVKYLNNLIEQ"
BASE COUNT          122 a           66 c           76 g           96 t
ORIGIN      
        1 ttgggtgcaa gagtacagca aagtcctcta ttatctttgg aagaagaaaa atagacaatc
       61 cttctattca tggaaaatgg acgaaaccta tatcaaaatt aagggacgtt ggcattatct
      121 ttatcgtgca attgatgcgg acggcttaac cttagatatc tggttacgaa agaaacggga
      181 tacgcaagca gcctatgctt tcttaaaacg actccataaa cagtttggtg agccgaaagc
      241 aattgtgacc gataaagcac cttctcttgg ctccgccttt agaaagttac agagtgtggg
      301 tttatatact aagacagagc accgaactgt gaagtatctt aacaatttaa tagaacaaga
//
LOCUS       DAGCXG010000287          355 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.300, whole genome shotgun sequence.
ACCESSION   DAGCXG010000287 DAGCXG010000000
VERSION     DAGCXG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.300"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
BASE COUNT          125 a           57 c           60 g          113 t
ORIGIN      
        1 aaaagtgaga aaaaaggctt ataattggag tggtagttaa ttagtgactt atttttgatt
       61 ttatagcact gatactataa aatatagata tcatcatatt acacaatctt aatactaact
      121 taaaaaatat ctcctttcac aagtatggtg ataaaattcg ttccgggcta cctttttagg
      181 tagcctactt taatctttat acctttctgg atcaacgaaa gtatacttta tatagtcata
      241 acgccgatga tcgctacgtg cgtccggcac gtcagtcatg aatcggctac actagactag
      301 acagaaaaaa taaggtgtgt agaataataa aaaacacact ggaggatttt tcatg
//
LOCUS       DAGCXG010000288          327 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.302, whole genome shotgun sequence.
ACCESSION   DAGCXG010000288 DAGCXG010000000
VERSION     DAGCXG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.302"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
BASE COUNT          127 a           37 c           67 g           96 t
ORIGIN      
        1 aacaattttg tggagcgtga agcttttatg gtagaggaaa atatgaataa agtgatgttg
       61 ataaaaagat tcctatcttg aatatgaatc gagatgtctt gaaaatgaat gagtttgacc
      121 aatcattatc ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag
      181 taaaaaagaa tatgttctag gaagagggcg gcacatctaa atttttaagt aacggacagt
      241 tcaaatatca agcattgaaa gaggctatcc tactgtttga aggaatacga atatataaaa
      301 acgacaaaga ggaaaaagta gttcatt
//
LOCUS       DAGCXG010000289          306 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.304, whole genome shotgun sequence.
ACCESSION   DAGCXG010000289 DAGCXG010000000
VERSION     DAGCXG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.304"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            1..306
                     /locus_tag="MB022_002910"
                     /pseudo
     CDS             1..306
                     /locus_tag="MB022_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          129 a           54 c           58 g           65 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagcc
//
LOCUS       DAGCXG010000290          299 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.denovo.305, whole genome shotgun sequence.
ACCESSION   DAGCXG010000290 DAGCXG010000000
VERSION     DAGCXG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.denovo.305"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..>299
                     /locus_tag="MB022_002911"
     CDS             <1..>299
                     /locus_tag="MB022_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4467821.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAGCXG010000291        38276 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.018, whole genome shotgun sequence.
ACCESSION   DAGCXG010000291 DAGCXG010000000
VERSION     DAGCXG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.018"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            177..1895
                     /locus_tag="MB022_002912"
     CDS             177..1895
                     /locus_tag="MB022_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBT4467822.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            1897..2751
                     /locus_tag="MB022_002913"
     CDS             1897..2751
                     /locus_tag="MB022_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4467823.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            3427..3747
                     /locus_tag="MB022_002914"
     CDS             3427..3747
                     /locus_tag="MB022_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467824.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            3771..4181
                     /locus_tag="MB022_002915"
     CDS             3771..4181
                     /locus_tag="MB022_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4467825.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            4328..4534
                     /locus_tag="MB022_002916"
     CDS             4328..4534
                     /locus_tag="MB022_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4467826.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            4881..5402
                     /locus_tag="MB022_002917"
     CDS             4881..5402
                     /locus_tag="MB022_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467827.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            5460..5885
                     /locus_tag="MB022_002918"
     CDS             5460..5885
                     /locus_tag="MB022_002918"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4467828.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            6064..6294
                     /locus_tag="MB022_002919"
     CDS             6064..6294
                     /locus_tag="MB022_002919"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBT4467829.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            6328..6510
                     /locus_tag="MB022_002920"
     CDS             6328..6510
                     /locus_tag="MB022_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HBT4467830.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            6623..8134
                     /locus_tag="MB022_002921"
     CDS             6623..8134
                     /locus_tag="MB022_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467831.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            8457..8681
                     /locus_tag="MB022_002922"
     CDS             8457..8681
                     /locus_tag="MB022_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBT4467832.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            8674..9240
                     /locus_tag="MB022_002923"
     CDS             8674..9240
                     /locus_tag="MB022_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBT4467833.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            9228..9458
                     /locus_tag="MB022_002924"
     CDS             9228..9458
                     /locus_tag="MB022_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467834.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            9608..10024
                     /locus_tag="MB022_002925"
     CDS             9608..10024
                     /locus_tag="MB022_002925"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HBT4467835.1"
                     /translation="MNKPVLFVYRKKKEYKKMSRAKNPKYESMSLEELKANMEKSRQE
                     LEAAIHHKNILEQRKKLVERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQN
                     SSYNKSIVRQAHIAALHKDQQQIAEAVKKTEKGDDS"
     gene            10024..11751
                     /locus_tag="MB022_002926"
     CDS             10024..11751
                     /locus_tag="MB022_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBT4467836.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            11781..13697
                     /locus_tag="MB022_002927"
     CDS             11781..13697
                     /locus_tag="MB022_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4467837.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            14382..14564
                     /locus_tag="MB022_002928"
     CDS             14382..14564
                     /locus_tag="MB022_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467838.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            14591..16288
                     /locus_tag="MB022_002929"
     CDS             14591..16288
                     /locus_tag="MB022_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4467839.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            16313..16444
                     /locus_tag="MB022_002930"
     CDS             16313..16444
                     /locus_tag="MB022_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467840.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            16536..17399
                     /locus_tag="MB022_002931"
     CDS             16536..17399
                     /locus_tag="MB022_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467841.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            17436..17972
                     /locus_tag="MB022_002932"
     CDS             17436..17972
                     /locus_tag="MB022_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBT4467842.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            17986..18408
                     /locus_tag="MB022_002933"
     CDS             17986..18408
                     /locus_tag="MB022_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4467843.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            18338..20737
                     /locus_tag="MB022_002934"
     CDS             18338..20737
                     /locus_tag="MB022_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4467844.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            20769..22760
                     /locus_tag="MB022_002935"
     CDS             20769..22760
                     /locus_tag="MB022_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4467845.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            22783..23034
                     /locus_tag="MB022_002936"
     CDS             22783..23034
                     /locus_tag="MB022_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBT4467846.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            23024..24253
                     /locus_tag="MB022_002937"
     CDS             23024..24253
                     /locus_tag="MB022_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBT4467847.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            24250..26331
                     /locus_tag="MB022_002938"
     CDS             24250..26331
                     /locus_tag="MB022_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4467848.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            26480..30400
                     /locus_tag="MB022_002939"
     CDS             26480..30400
                     /locus_tag="MB022_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBT4467849.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            30401..31345
                     /locus_tag="MB022_002940"
     CDS             30401..31345
                     /locus_tag="MB022_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467850.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            31349..31552
                     /locus_tag="MB022_002941"
     CDS             31349..31552
                     /locus_tag="MB022_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBT4467851.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(31852..32088)
                     /locus_tag="MB022_002942"
     CDS             complement(31852..32088)
                     /locus_tag="MB022_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467852.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(32271..32657)
                     /locus_tag="MB022_002943"
     CDS             complement(32271..32657)
                     /locus_tag="MB022_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBT4467853.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(32639..33967)
                     /locus_tag="MB022_002944"
     CDS             complement(32639..33967)
                     /locus_tag="MB022_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4467854.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(33928..34257)
                     /gene="mobC"
                     /locus_tag="MB022_002945"
     CDS             complement(33928..34257)
                     /gene="mobC"
                     /locus_tag="MB022_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4467855.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(34515..34886)
                     /locus_tag="MB022_002946"
     CDS             complement(34515..34886)
                     /locus_tag="MB022_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4467856.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            35031..35216
                     /locus_tag="MB022_002947"
     CDS             35031..35216
                     /locus_tag="MB022_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4467857.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            35374..35541
                     /locus_tag="MB022_002948"
     CDS             35374..35541
                     /locus_tag="MB022_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467858.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            36159..36818
                     /gene="vanR"
                     /locus_tag="MB022_002949"
     CDS             36159..36818
                     /gene="vanR"
                     /locus_tag="MB022_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4467859.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            36821..38164
                     /gene="vanS"
                     /locus_tag="MB022_002950"
     CDS             36821..38164
                     /gene="vanS"
                     /locus_tag="MB022_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4467860.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILEPVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT        10868 a         8603 c        10048 g         8757 t
ORIGIN      
        1 gtaatgaaaa ctatatgtca gcaggtgaaa aatgaataag tcaattataa cattataaag
       61 atttccattg atttttacac ctattttaca caagcactca catagatttg atttctgtct
      121 tctataattg tataggaaaa tgtagatctt atataataac aggagaggaa agagaaatga
      181 acgagaatgt gaaagtggtt tttggtctga tcggtggcct tgccttgttc ctttatggaa
      241 tgaacagcat gagtgatgca cttcagaaag ccgcaggaga acgaatgaaa aaaatccttg
      301 gatttcttac aagaaatcca attatgggag cacttgccgg agcacttgta acagctgtat
      361 tgcagagcag ttcagcgacg acagttatgg ttatcggttt tgtcagtgcg ggacttatga
      421 gtcttccgca ggcaatttct gttatctttg gtgccaacat aggaaccact atgacggcac
      481 agctgatggc atttaaaatc agcaattata tttatccaat tatctttgtt ggatttattc
      541 tgaactttgt tagcaaaaaa gaaaaggtta aaaatatcgg tatggtaatt ttttcattcg
      601 gacttttatt tgaaggaatt gaaatcatgg gtgaagtcat gaaacctttg gcgggaagtc
      661 cggtctttgt tgatctgatg ggaaaagttt catccattcc ggttcttggt gttgtactgg
      721 gagcggtcat gacattggtt gtgcagagta gttcagctac gattgcagtt ctgcagaatt
      781 ttgcatcaca ggcaggaccg gacggagtaa gcagtgtaat agggcttacc ggtgcgattc
      841 cgattcttct tggagataat ataggtacaa cgatcacagc acttcttgca tcaattggac
      901 aatctaaaaa tgcaaaacgt acagcaattg cgcatagtat ttttaatatc agtggaagct
      961 gcgtttttat tttcctggtt ccgtggtttg caaaatttgt ccagtttatt tcaccaaagg
     1021 gaaatgagat tgatgtgatt tcaagacaga ttgcaaatgc acatacaacg tttaatatcg
     1081 tctgtacact ggtctggctg ccactgattc cgctgatggt aaaaattgtt acaacaatca
     1141 tccgtggaaa tgacaaaaca gagaaagcag cttttgaacc caaatatctg gatatgaaag
     1201 tgattgaaca gccggcggct gctatggttc tggtatctaa ggaactgaac cgacttggag
     1261 aactggcaga atctctgtta agtgatttaa aaacggcgat tgtagcagat ggagactcaa
     1321 aaactcatgg ttcgtttata gaaaatctgg agattgtaca tcagttacag gacagtgtga
     1381 cagaatatat taccagatta ttcgcaagcg gaaaccttac agaacaacag tctgagcaga
     1441 cggcagggtt actgtatgta aacaatagta ttcagaggat tgcagacaga tgtgaagata
     1501 ttgaccagat atgtgaaaaa gtaaataaca atggaaaaag actgaccagt gaagcctcct
     1561 cagaaatgga agactgcatt gatattatcc agcagctttt aaagaaagca atggaggcaa
     1621 tccgtaaggg tgatgctgta gtggctgaaa cagtttttaa aaataagaaa aaaatgcata
     1681 aagcagagaa aaaatacagt aaggcacatt taaaccgtgt aaaaaatgag gtttgtgatg
     1741 cgtctatgac aagatatttt tctgggatca tgtataattt tgaccgtatg gcagataatt
     1801 gtgtcagcat tgcagaagaa gcctgtgata atgtggcttt tataaacctt gatgaagaga
     1861 cgggaaaatc aatgatggaa agaggtgcag tataaaatgg taaagaaaaa aaacatccga
     1921 aaatctgcac ttctgctgat aatgataatg ctggcaacgg gaataacagc atgtggaacc
     1981 gaggaagagc cattgccgga tctgtctgct atgggagctg tcacagctgt atcacgagaa
     2041 gaagggtcag gaaccagagc agaatttgag aatcttttga aactgcctga gagtgataca
     2101 ggaatagttg ttgattctac agagaaagtt ctgaaaaagg tggaagaaga taaaaacgca
     2161 gttggttatg tcgcatatag ttccgcaaca gatacgaatg gaaaaatact tcaaatcaac
     2221 ggagttcttc cttctgagaa aacaatagac aataacagtt atcctctttg cagggattat
     2281 tatctggctt ataatggaga actgacagat gtggaacagg atttcctgac ttatgtaaag
     2341 agtaaaggac aggatattgt gaaacaatat tgtattcagg ctgacagtac aacaacattc
     2401 ctttctgaca agtcagaagg aaagattctg attgaaggat ccacttcaat ggaaccaatg
     2461 gtaaaagcac tggcagatga ctatcagaaa caaaatccaa atgctgaaat agaagtaaaa
     2521 gcaacagatt cttcaagagg gattacggcg gtgataagtg gagagtgtga tttcgcaatg
     2581 tcatccagag aattaaagga ttatgaagca gagcttctgg aaacaaaagt cattggaaaa
     2641 gatgctatag ctattgtgat aaatgaagaa aatccactgg aaaatcttac aataaaacaa
     2701 cttacaggtc tgtacaatgg aacatataaa aactgggatg atctttcctg agaaaaacga
     2761 atataatatc ttacaagcac tgactataac ggtcggtgct tcttttttgg attttttcaa
     2821 aattatctga cattcttcaa attcccacgt gtgattagtg gaaaaataat tttttctgca
     2881 aaagtaccgt ccaaatgccc tctgaaactt gctattagtg agggaatatt tcatttatgc
     2941 gaaaaaaact tgcaaaaggg ggttggtaaa taccgcttcc caggctttat aggtagagga
     3001 acaattattt taaaaatttt accccaaaga tgtccgcttt tgcctgtctc agtacagagt
     3061 atgtgaaggg actaacaaaa gtaaaagaaa aaatgataaa aatgcaaaaa aatatcacga
     3121 atcttttgca tcttgcgaag tcatttgact ctagtaatca gaggacgttt tggaatgttc
     3181 cttcgatgaa ccttgacaac cgataagtgc accctagaag cctaacagga gttatgccat
     3241 gacctttctt tttctgagag cgagcgaaat tataatgaca ctcccgatga tgggcggctg
     3301 accgaagcct tatggtcagt gacctgctga tgcaggcaga ggcagtctga gacggctgca
     3361 ggtgacaggg aaacgcacgc cagaccgccg gacgaatgaa actgatgaca atgtttacag
     3421 ggcaaaatgc ccggaaagga gcagtttatg acagaactga aaatgaaaga aatgcagttg
     3481 attaactggt ttggatgccc gaatctgatt tatacaaggg agagaatcca tctggttgcc
     3541 atgcttacaa cagatgaaga aatgaaaaag aatctttaca gggtatgcag attccttgca
     3601 agagaagaaa tcgcatatcg gtatccgatc atgtatgaaa tgatccggaa aatattttca
     3661 ccggatgata ccaatccacc ggaagcatgt gcaatgttta tgccggacga tgaaacagat
     3721 gatgaagaat atgcaatggc agcgtagctg cggaaaggac agtaacgatt atgaataaag
     3781 tggttttaat gggaagactg acaaagaatc cggagatcaa atatgccgga aaagataatg
     3841 atatggcagt agcaagatat acactggcgg taaacagaag atataaacgt gacggtgaac
     3901 aggaagcgga ttttatttcc tgtgtcacat tcggaaagag tgcagagttt gcacagaaat
     3961 atcttcacaa gggaatgagg attgtgattg gcggtcgtat cagcactggc aattataagg
     4021 acaaggacgg gaaaacaatt tatactacag atgtgatcgt ggaagaacat gaatttgccc
     4081 agaacaagga taatggtgtt ggagctgatt catcggaaac accgaaaaca gataaggacg
     4141 gatttatgga agcaccggaa ggtgaggttc cattcgacta aaccgaagta aatggttgga
     4201 gaggttgccc tttgtcgggg catcctcttt tccattttac agaagacatc aaaaattaca
     4261 ggtattcaac ctaacgaaat cgtgatataa tcaaaatatg ttcagatgaa aaatatggag
     4321 gttgcttatg ggcgtaagtt ataacagatt atggaaaatg ctcatagata aaaatatgaa
     4381 gcggatagag atgcagtacc tcaccgggat cagtggaaat attcttgcca ggatgggaaa
     4441 aaaccagtat gtatcaatgg agacgattga gaagatctgc aagaagctgg actgcaccgt
     4501 tgatgagatg ctggaattta cagatgatga gtaacaagag cagacatgtc aaattattaa
     4561 aactggaaga atgcttcaga agtcgaaaaa agaaattgaa aaaagataac acgccggata
     4621 cggcataaat ctcttagcaa cgctaagagc gcacttctat acaggcaatt ccatctgcct
     4681 gtatcgtgcg acctgcggtg ctgttcccga tttcaagaaa tctcaaaccg ggaaatacag
     4741 cgaaatcaga gatttcgtac aaggggaaga tccccttgcg gcacagatgc ctatccctga
     4801 taaagtttgt gactgaatat ttcagatata gacatataag gggaaagaaa gtgtgaaatc
     4861 aaaatttgtg gagagatttt atgattagaa aagcaatact aaaagatgtt aattcgattg
     4921 aagatactta caatgagcat ttccagtatg aactgaatca tacagccttt actgtattta
     4981 agaaaggggt ttatccaacg aaagatgatg cagaacgggc tatttatgct ggtgctttgt
     5041 ttgtatatga agaaaacgga acaattgttg gaagtatcat tattgataaa gttcaaccaa
     5101 tagaatatgc aactattcct tggaaagaaa aattatctga agatgaggta atggtaattc
     5161 accttttaat ggtacgtccg agtatgtctg gtaaaggaat tgcatcatca ctaataaaat
     5221 tcgctacaga attagcacaa aaaaatagtt gcagggcact acgtttggat acaggaagtc
     5281 aaaatattcc tgcgctatct ttgtatcaaa aaaatggatt tgaaataatt gctagtgctt
     5341 ctaaaaaagt tggagatgta attgcacaca aaaaccatct ttttctcgaa aagatattgt
     5401 agttcgtaaa ttccagtttg aaaaattgag aaaatcggag tttgaggagc atcaccgtta
     5461 tgattagaga attacggaaa gtggatataa ataaagttgc agaaatatgg ttagatacaa
     5521 atataaagac acattatttt atctctgctc aatattggaa aagtaatttt gagttagtaa
     5581 aagaacttct attacaggca actgtctatg tttacgagga taaacaggaa atacagggtt
     5641 ttatagggtt aagtaacgaa tacattgaag gtatttttgt ttctgccgaa atgcaatctc
     5701 aaggtatagg taaaattttg ctaaactatg taaagggaaa aagaaacaaa ttaatcttaa
     5761 atgtatatca aaaaaataca cgggcaatat ccttttatca aagagaagga tttgagattc
     5821 agtatagcgg tttggatgaa gctactggag aaaaagacta tgtaatggca tggcagcaga
     5881 aatagaaatt caagttttag tgtttggaaa attatgactt agtgtgcaag ttgggagaaa
     5941 atatggtaac ggaaaaagag aaaaaagcat gtcgatttat tgccgaatgg ctataagatt
     6001 gtgagacagt ctctggtaga tgatctggag ggcgaaaaaa aggtatgttg ggagacagaa
     6061 attttgcagg atgatgatag tggatttact tatgaaatca gcaggtgtct gtatttcgat
     6121 acctgtaagg aacatggcta tgcagagttc tgcaaagtat tttgcaacca tgactggtat
     6181 gcttatggag tattaaagca tcattcgaga tttatcagaa aatcgacgat tgccgaagat
     6241 ggaactgtat gtcatgatac aatcgagaaa gtaacagaga aacatttttt ataaaaccga
     6301 aaaattgatt atgggaggaa attgaaaatg gcgaaagaag agagatttga aaaaatatat
     6361 tctcagggag cattaactac tactgagata tgggtggata aggaaactgg tgtgaattat
     6421 ttatatcatg catgtgggta ttcaggtgga ctgactccat tgttggacag agatgggaaa
     6481 ccagttattt ctcaggctat aaatagatga ccttagaaac agcaactgtt atggtaagta
     6541 acagcatcgt cacaatatgt atatggtcga ttggtgtgtg cggtaatata ctatattcag
     6601 aaaaacaatt caggagattt ttatgagaag atcaggttgg acaaaatggt tgatcgtgat
     6661 tccggcaatg ctaggcattg tgttggtgac ctatgtgcgg tatacaacgg atatctgggg
     6721 atttggtgca tttatctgcc agctgattgc aattttggaa ttggcttatg gtctgcgaat
     6781 tgcgatgctg gcacagaata gaaagaagtc ctatcggtta acaccggaag aacgacatga
     6841 gtatgcacgg tatttatatg agaagcaata tcaccgttac cctgctgtag cgaatcagat
     6901 gcttttggtt atggcacgga tgagtgtact ccttaacaat tatgaacggg ctgcacagga
     6961 gctggcagat attcgtatag ataaattcaa tccagcacag ttgaagttgt attattacct
     7021 gaaagtggtt acagcaatgg cagcaggaga tgcgacaggg attcaagaga gccagatgtg
     7081 ttacgcaggg attccggata ctaaatgcac atatccgtca aaagcagagc ttgctatgtg
     7141 gatagagcag agagcggcag cgcagatggc agaggcactg aagaaggcag tgccggataa
     7201 aaaagagcat ccgatacgga tcagtatcat tactttggtt cttgcgtata gtaccttctt
     7261 ctatggattg tggtatggta taaaacgtga tatgggatat gaagtgagat atcgtttcgc
     7321 agaaatatcg attactgtta ttacaatcag tctggcggtt cttttgatct gggggatcgt
     7381 gagtttatac agacacaatt gccaggcatt atccatacaa ttcggtaaaa acaaagtggc
     7441 attgataatt gtctatggag ctgctgcgat atttgcactt ttgattattg gaatgagtgg
     7501 tctgagtgta gtgttcggaa tggatggaac agaaacggtg actggttgtg acagcaaata
     7561 tacgtatata actgtgcagg aagattatgg tggtgaacgg agatacagaa ccaataatcc
     7621 atttgttatg cagagtatgg atggtctgtg gtggtcttcg gatacaatag atgaagaaga
     7681 taatcaagaa gaggatacta cagaattacc ggatttggat acagagaatt caacagattc
     7741 agaggatatt cagagtggaa ataccataga agattacgag acagaagggc agcatgatgg
     7801 tctgacaatt caaaatgcaa tgctggcagt ttataattac cttcaggagc agaatacact
     7861 tcaaaatatg gcgttcagtt acacggcgaa tgcaaaggga gaagtctatg ctgtcgtcag
     7921 cgaaacgcag gaaacaaaga acggtaatac ggtaaatgtg agatactgtc tgtacgataa
     7981 tggttccaag acagatgcag atggtaattc ctgcgaagaa ctggtgttag aaaaagtata
     8041 tccggatggc aattacgaga ccgaattggt agatttttat ctggtaaatg cagatacgat
     8101 gcaggtgaca gacgaacaga agaatacgtg gtaaaataaa ttctggggaa gtaggtgcat
     8161 tgtatgacaa tgagagaaaa aataaaacta ttatcgtaaa tgtttcaagc ggactgggtt
     8221 cttttggaag tatgcaaaag gactccaaga cataagaggt gaactgcgct gatccaggtg
     8281 ccacggcaac agatttgaat gtcgacggag gaaagcaaac agtacaggcg caatcgtaaa
     8341 acttgcaatg attgataaaa ccggactaac agtaactttt attagtcgat ttggaaatat
     8401 gccttggtaa tcaacaattc cggttttact gactaagaaa atgaggagga ttcataatga
     8461 gtgagaaaac agagattact tttatgcaaa caagactaat ccggcttgct tcagaagagt
     8521 ggcatttacc tgttgaacag attatccact tgtttaaaga agccgatgtt cttggatata
     8581 tagaaaaatg ctatgggatt tttcattgtg aaggcgatga agctgtgctt gaagatatta
     8641 ctgaattttt gcaaagaaag gggatagaga ttagtgcttg agttaaaaga tggatttgtt
     8701 ctctatcatg gaagttattg tgaagtaaaa gagccagatc ttgccaaatg tgcaaagaga
     8761 aaagattttg gacagggatt ttatttaacc acatcaaagg aacaggcaga aagttttctg
     8821 agaacctcaa tagccaaagc catcgcaacc ggaacaattg aagaggggca gaagtttgga
     8881 tatatttcaa cttttgaatt caaactttca gaaaatcttg aaacgcatat ttttgaaaat
     8941 gcagatgtgg actggttaca ttgtatcgcc gctcaccgaa agaaaaaaat gtttattgag
     9001 gttgaacgtg aaatggcaaa atatgatatc attgcaggaa agattgcgga tgatgcaaca
     9061 aacgctactc ttaccgctta tcttgcgggt gcttttggga ctgcaggtga taaagtagca
     9121 gacgatttct gtatcagaca gttgctgccg aataaattga aagatcaata ctgctttaaa
     9181 actgaggcag ctattgagtg tctgaaattt gtgaaaggtg agaagatatg gctgaagtaa
     9241 caattccaaa ggaaaaaatg aattatacaa tcgaccttct gatcactatg gtgacagatg
     9301 aaatcgcaga agagacaggc aaggacagaa acgaggtatt gacagatttt ttgtgttcaa
     9361 agacaggaaa agcactgtat gatgagaaga caaagctgtg gtgcactggt ccggcatata
     9421 tagctgaact gtatatggaa gaattaaaga aatcctgata tgggatgtga gtttaaattt
     9481 atacgacaat ggtgacaaag actttcattc caaggagcaa caattaaata tttcaaaaac
     9541 gctgaggtca gcatttccta atcacaggac aacgacctca gttttttata cccaggaatc
     9601 ggcattcatg aataagccgg ttctttttgt ttacagaaaa aagaaggagt acaagaaaat
     9661 gagcagagca aaaaatccaa agtatgaatc catgtcattg gaggaactga aggccaacat
     9721 ggaaaagagc agacaggaac tggaggcagc aattcaccac aaaaacattc tggagcagcg
     9781 gaagaagctg gtagaacgca gggaacgttc ccaccggctg attgtcaaag gggcagagtt
     9841 tgagaaagta tttccgctgt ccagagatct ggaacaggaa gacgtccagg acatgatgaa
     9901 tcaactgcag aacagcagtt acaacaagag tattgtgcgg caggctcata tagccgcctt
     9961 acacaaagat caacagcaga ttgcagaagc agtaaaaaag acagagaagg gagatgacag
    10021 ttaatggcac tgtatcattt ttcagtaagg aatgtatcca gagggaaagg gcagatggtg
    10081 gtcgcttccg cagcctatat ttccgggcag aggatttttg acagttatta caacaagata
    10141 cacgattata cgagcaagtc aggtgtgata tttacagaaa tcctgacacc ggagtatgtg
    10201 ccagagcgtc tgactgacag ggaaacactc tggaacgagg tggagcatgt ggaacggaac
    10261 aacaaggcac agcttgccta ttcgtttgac attgcactgc agaatgaatt gacacttgag
    10321 gaaaatatca gactggcaaa agagttctgc caggagcagt ttgtggcacg tggcatgatc
    10381 gtggatctgg cagtccatga gggaaaagcg gaaaatgaag aagaaccgga taatccccat
    10441 ttccatgtgc ttgtcccaat ccgtcctttt acagaggaag gaatctgggg caataagcag
    10501 agaagggaat atattcttga tgaggatgga aaccgggtga aggatgccaa aggaaaagat
    10561 atgttcaatg ctgtttctac taccggatgg aatgatccgg agcttttaaa agaatggcgg
    10621 cgggcatgga cagagaaagt aaatgggaaa ttcagggagt gccacatggc atccagaatc
    10681 gaccaccgtt cctacaaaga acaggggatt gacctgatcc cgaccatcca tgagggatat
    10741 gaagtccggg caatggagaa aaagggaatc aaaacggtga tcggagaatt gaaccgggca
    10801 atccggcagt ttaaccagat gtttatttct ctgaaagaat ccattcagtg gatgaagaca
    10861 gcttatgagg aaatgaaagc agagctggat cgcaggcaga acccgacact gctggaaagc
    10921 ctgcaggatt attatgataa aaagacacag ggcagaccac cacttcccaa tttttatgcg
    10981 gagatgaaac agagaggtaa gaatctctcc aatttacagg agtttgcaaa atccatcagt
    11041 tatcttcaga cacatcagat agaaacgatg gatgacttgc aggaacggat tgaggaactg
    11101 aacggtgttg tttctgtcag taaaaaagaa atttctgaaa agcggaaaca gttaaaggaa
    11161 cttgagaatc tggaaaagat ggcagaggta ataaaaacaa atcagccatt gattgatgag
    11221 tataaccatt tctattttca gaaaagacgg gagagatatt atcagcagca taaaaaagaa
    11281 atcaattatt accgcaaatg tgaacgggaa ctgaaacaac atctggataa aaatggaaaa
    11341 gtgccaactg ccagatggaa acgggagaaa gaagaacttc aggcggggat cgaagagctg
    11401 aaagcagata atcagccgta ccaggaggaa ctaacttttg tgaaaaaggt acagagctgt
    11461 gcggatattg ccagacgtga ccgggaaatg gcagaagcag atacttccgg gcgttcagaa
    11521 gaaaaaaggg agaagcaggt gaagttcccg acattttaca cagttcaggc gaaagagatt
    11581 cttgaagaaa acgggaaagc agagcaacag ccgttgaacc agtcggaaca gaatccagaa
    11641 aagaagacct ctcttctgag aaaactggat gaaaaaaaga aagagtgtgc agagcgtgat
    11701 gcaaaacaac aggcagtgaa gaaaaagcga aactatgaca tgagtttata acagtctgtc
    11761 agcaggacag acgggaaagg atgagcaaaa tgcagtatac agaagaacaa attataagag
    11821 caaatcagac agatcttgtt tcttttctga gcgcacaggg agaacaacta gggaaaagcg
    11881 gaaaggaata ccgttggaaa aagcatgaca gtgtaacggt atccggaaac cggtggtaca
    11941 ggcacagtca ggggaggggt ggctatccgg tggattttgt gatggaattt tacaatgcca
    12001 catttccgga agctgtcaag atgctaaccg gggaggaagg cgaaggcagg aacagtacct
    12061 gccccgcccc gtcccctgac ttccgattac cagagaaaga ggaaaataat gatagaatca
    12121 tcagatatct gacagaaaac agagggattg aaaagaatat ggtggaggaa tggatcggca
    12181 gtggggatat ttatgaagaa aagaagcacc ataatgtggt gtttgttggc agagatgcag
    12241 acggtattcc aagatatgcc cattgcaggg gaactggtga aacaaaatac cggggagatg
    12301 tggctgaatc tgataaatct tacggattct gtcacagagg gacggataat cagttgtttg
    12361 tgtttgaagc agccattgat ctgctttctt ttatccaatt atttcccaaa gactggaaaa
    12421 agcggagcta cctgagtctg ggtggcattt ccagtgcggc actgatggct tttctttctg
    12481 aacgccctca gattacttcg gttttcctgt gcctggataa tgaccatgca gggaatgaag
    12541 ccagtgagaa attggcaata gaaatcccgg acggatacag tgtcatccgt ctgaaaccgt
    12601 cccgaaagga ctggaatgaa atcctctgtg ataaaaatgc agacaggaag aaatcaatca
    12661 ttgaaacagt tacaatgaaa gtaccggaga aggaagaact ggtgccgatg ttgtgttatg
    12721 aagacattga acagaccagt gtagaatggc tgtggtttcc ctatctgcca tttggaaaac
    12781 tgacgattat tcagggcaat ccaggagagg gaaaaactta ttttgccatg atgctgaccg
    12841 cagcctgcac gaaccgtaag actttcccga acatggaaga gattgaacct tttaatgtga
    12901 tctatcagac tgcagaagac ggtatgggag atacgattaa gccgaggctg gtggaagcag
    12961 gagcagacct gagtcgggtg atggtcattg atgatacaga ggaagcattg acgttatccg
    13021 atgaccgtat tgaaaaagca atccgtcaga atcaggtcag acttttaatc attgatccgg
    13081 tacaggcttt tatcggtgcc gatgtggata tgaaccgggc aaatgaagtc cgtccggtat
    13141 tccggaaact gggaatgatc gctgaaaaga ccggttgtgc gattgtgctg atcggacatc
    13201 tgaataaatc ttcagggaca cagagtactt accggggact tggttctatt gatatcatgg
    13261 cggcggtgag aagcctgctt tttatcggga aagtaaagaa agacccgaca acaagagtac
    13321 tgatccatga gaaaagttct cttgcacctc caggtgagac gatggcattt aaacttggag
    13381 atgaagaagg attccgctgg gtgggagctt atgagatttc cgcagatgat cttctggacg
    13441 gaaaggaagg aaagccgaca gaaacaaaac tgcaacgggg aacgaaactg atctatgaac
    13501 tgcttgcaga cggaaatgca gtcacaatcc gggaactgga tgaaaaggca aaagcacagg
    13561 gaatctccca gagaaccatg cgagaagcca gaagccggat gaaagaagaa ctggattatc
    13621 ggatgaatga aaagcaggaa aataccattc gattaaaaaa acaagggaga atgggtgacg
    13681 gcagaatttt ggagtgaacg gcaggcttta tagataccct atctgccgtc tgccgtctgc
    13741 cggtgcaagg caatcaaacg gaaatagcag gtatccatac agaataagga ttgctgtgaa
    13801 tctggaacaa aaatgaattg taatatggcg gatataatgg tatgattatc cggtaacagc
    13861 aatgttacgg ggaagcacct gtgacagggt gtggctgcct cctgatattt acagaccgga
    13921 gcctttcggg gttctgagat gaaagaaagg aggtgcgtga tatgacagct tatgagatta
    13981 tctcaatatt cttaggcgta ctcagtttac tgatcgcttt tggtagcctg ttaattgcga
    14041 ttattgcctt tcttgatcgg aagaacaaga ggaaataaaa atgcctatcc tgtctgccaa
    14101 caggataggc ggtggtcgta agaccattta gtcaactcag gcggcatcca ctttggagtg
    14161 ggtgcttttc ctataacaaa tgtaccacac attcctcttg ttttcaatgg gaaaattaaa
    14221 atatgcagtt catctgacag ggatttatcc ctgtctttat tatatcccca atttctgaaa
    14281 tgttaataca aaaattttaa tatatgtaac caacagtctg ggcagttaat ctgtaaaaat
    14341 ggattagctg ctcttttttt gcaaaaaatg gaggttttac aatggcagtg aagaagaaaa
    14401 ttatcaacat cagagtatat tttgacggtg atcaggacgc aaaagaagtg tttgcagagg
    14461 tgatcgcaca gaaaatacgt gacacgaaaa caaaagataa acttgcaata atgaaagata
    14521 agcagtataa tgattatgag ttcagtaatc tgaaatctgc gtaggatgtg caaagtaacg
    14581 gaggtatcag atgaacaaca gatatatgag taacaatata gaaagaaatg caacctggag
    14641 agttggtgta tactgccgac tttccaaaga tgacgaattg accggagaga gtgccagcat
    14701 ctccaaccag agagacatac tggtgaatta ctgtcagtca cagggctggc aggtggtgga
    14761 tgtgtttcag gacgatggtt atacaggatt gaacacagac cgacctgatt tgcagagact
    14821 tctcaaagcc tgtgaaaaag gactggtgaa tcttgtaatt accaaagacc agagccgact
    14881 gggacgaaac catgtacaga caggatttct gatggaggag tttttcccaa agcatggagt
    14941 aaggtatatc gcattatatg ataatgtgga tacctttgcc ggagacaatg agattgcccc
    15001 atttaagaac gtgctgaacg agatgtattc cagagatatt tccaagaaag tccatgcgtc
    15061 ctatcacctg caggcaacca agggaaagtt tacaggtgtt gttccaccac tgggatatca
    15121 gaaagacccg gaggaaaagg gacatctcct gattgacgac gagacagcac cgattgtccg
    15181 gaaaattttc cagtgggcgg tggacggtca tgggattaac tttatatcca gagaactgga
    15241 acgccagcag attccctgcc cgtcatggtg gcaccggaaa cggggactcc gcacctatta
    15301 cacaaaatgg gaaaagaaag acccggaaaa tgggaaatat gtatgggatg aatcctatct
    15361 gaaatccctg ctgatgaatc ccgtttactg tggtgacatg gcttcccaga aaaggtacta
    15421 ccgtttcaag attggcaatc agggagataa agctccggag gactggatca cagtcaggga
    15481 tacccacgaa gcaatcattc cgcaggatgt atttgatctg gtacaggcaa agatgaaagg
    15541 caggaaacgc cagacagaac agggagaata cagcatgttt gccggattac tccgctgtgc
    15601 agagtgtggc ggtgcgttga cgttgaagaa gacgcacacc aaagaccagc atgaagttta
    15661 tacctgttcc acttatatcc ataaggggaa agcacattgt acccagcacc gggtggacgc
    15721 tgatgacctg tatgatgcag tccttaccag aatccaggaa tgtgcaaaag cagttaccgg
    15781 agacggaaca gaactggaaa accgggtaaa ggagttatgc gaggaagata cacagggaca
    15841 cagggaaagt ctggagaagc tggtatcaaa gcagaaagac cgcctggaaa cgttagaccg
    15901 gctgattgca aaactgtatg atgaccttat caatgacaag attacagaat ctgtttttga
    15961 taagatgctg gagaagacac agaaggagca gacagatatc aaaaaggaac ttttccagaa
    16021 tgagagtgtg ctgaatacgg aagaaaaact ggatgcacag tcccagcagt ggattgacga
    16081 tattagcgag tatgcggaca tcaaagaact ggatgccaat cttctgaacc ggctgatcag
    16141 taaaatcgtt atctcagaac cgcaggagaa agacggaaca gaaaattact gccgtactcc
    16201 tgaaacggtc acaatggaaa ttcatttcaa cttaaaacca ataccggagc ttggaaccat
    16261 cgaaagaggt tctggctccc ataaataaga aaaatctatc aaaatttaat ctattttaat
    16321 cttttcaagg ctcatttcga gcaaatattt tctcataaaa gacataaaac agcttgtcgc
    16381 tggcggcggc gtggccctga tcggtgtcac tctggttccc ctgctgtccg ggctgctggg
    16441 ctgatcctgg cctttgtccc tgtccaaacc cttaactaat cgcctccgcg tcctcccggc
    16501 cagaccggga gggcgcggga aaggaggtgc tacccgtgat tagtgaaatc attgaagaat
    16561 ggataaaagg catcctcatg gacggtatca cgggcaacct gtccgggctg ttcgatacgg
    16621 taaatgccaa agtcggagaa atcgccgccg acgtgggacg aaccccgcag gcatggaaca
    16681 gcggtatttt caatatgctg cacagcctgt cggaaaccgt gatcgtcccc atagccgggg
    16741 ccatactcgc ccttgtcatg tgctatgagc ttatccagat gataacggaa aagaacaata
    16801 tgcacgactt tgacagctcc atgttcttcc gctggatatt caaatccgcg tttgcaatcc
    16861 tgatcgtaac gaacacatgg aacatcgtca tgggcgtatt cgacgccacc caaagcgtcg
    16921 tcaatcaaag cgccggtgtg attatcgggc agacatccat tgattttaac accctgatac
    16981 cagaccttga aagccaattg gaggctatga gcgtgggcgc tcttttgggg ctgtggttcc
    17041 aaacccttgt tgtgggcctc accatgaata tcctttccat ctgcattttc cttgtgacct
    17101 atggccgcat ggtggaaatc tatgttgtca cgtcgctggg gcctatcccc cttgccacaa
    17161 tgggcaacgc ggaatggcgc ggcatgggcc aagggtatct gaaatccctg ctggcgctgg
    17221 gcttccaagc ctttttaatc atggttgtgg tgggtatcta cgcggtgctg atacagaata
    17281 tcggttccgc ttccgacata tccggcgcaa tctggggctg tatgggctat acggtgctgc
    17341 tgtgcttctg cctgttcaag accggcagta tatcaaaggc cgtattcggg gcgcattgat
    17401 cccaaaatcc aagcatacac aggaggaact tgtttatttc aacgtacaac atcatttacg
    17461 ctgacccgcc gtggcggtat gactgcaaaa acgtacaggg cgcagcggaa aatcactatt
    17521 ccaccatgag cattgacgag ctgtgcgccc tgccggtgga acggctggcg tccaaagact
    17581 gcctgctgtt tttgtgggcc acattcccca tgctgccgga agctctgcgg ttaatcaaag
    17641 cgtggggctt ttcattcaag accgtggctt ttgtatggct gaaacagaac cgtaaaagcc
    17701 ttacatggtt ttacgggctg ggccgctgga caaggggaaa cgcggaaatc tgcctgctgg
    17761 caacacacgg caagccgaag cgtcgttctg cctctgtcca tcagtttatt atcagcccga
    17821 ttgaacaaca cagcaaaaag ccggatgtga cccgtgaaaa aatcgtagag ctggcgggcg
    17881 atttgccccg cgtggagctg tttgccagac agaaaacccc cggctgggac gtgtggggga
    17941 acgaggtgga atgtgacctt atcctgacgt gaaaggaggt gccaaatggc ttatgtaacc
    18001 gtccccaagg acttaaccca tgtgaaaagc aaggttgtgt ttgggcttac caaacggcaa
    18061 ctgatctgtt tttccggggc gctggtgatc ggcgtcccct tatattttct cacccgtgac
    18121 tatcttacca acagcgcggc ggccctgctc atggtatttg ccatgctgcc gggctttttg
    18181 cttgccatgt acgagcgtca cggccagccc cttgaagtgg tgattatgca gatcatccag
    18241 tgctgcttca tccagccaaa ggagcgtccc tatcagaccg acaacaccta caccgccctt
    18301 gtgcggcaat accagatgga acaggaggta aaggccattg tccaaaaagg aaatcccaag
    18361 agaaaccgaa aaaacaaagc tgacccgcgc ccagcgaaaa gaaattgacg cggtaatccg
    18421 taaatataag ggcgacggca ggccccacac ggcgcagcag agcatcccct acgaggtcat
    18481 gtacccggac ggcgtgtgcc gggtatcgcc cggcgtcttt tccaagtgca ttgagtttgc
    18541 ggacatcagc tatcagcttg cccagccgga cacgcagacg gctatctttg aaaaactgtg
    18601 cgacctgtat aactatgtgg acgcctccat tcatatccag ttttctttcc tcaatcggaa
    18661 agttgacccg gtgcagtacg ccaagagctt tgagatcgcg ccgcaggggg acgacttcga
    18721 cgacatccgg gccgagtaca ccggcattct gcaaaagcag cttgcaaacg gcaacaacgg
    18781 catggtaaag accaaatacc tgacattcac catcgaggcc gaaagcgtga aagcggcgcg
    18841 agccagattg aagcgcatcg gctttgacct gttaggctat ttcaagagca tgggggccgt
    18901 agcccatgtg atggacggct gggaacggct gaacctgctg catggcgtgt accacccgga
    18961 cggcgagata ttcaattttg actggaagtg gctggccccg tccggcctct ccaccaagga
    19021 ttttattgcc ccgtcctccc tgtgcttcgg caatgcaaag acattcggca tgggcggcaa
    19081 atacggcgcg gtaagtttcc tgcaaatcct gtccccggag ctgtcggacg atatgctggc
    19141 cgacttcctc aatacggaaa gcggtgtttt agtcaatctc catgtgcagg ccattgaaca
    19201 gaccaaggcg atcaagacca tcaagcgcaa aattaccgac cttgacgcta tgaagattgc
    19261 ggagcagaaa aaggccgtcc gcagcggtta cgatatggac attcttccca gcgaccttgc
    19321 cacctacggc gaggacgcca agaagctgct gacaaagttg cagacccgca atgaacggct
    19381 gttccagttg acgttccttg tcctcaacgt ggccgacacc aagcaaaagc tgaataacga
    19441 cgtgttccaa gccgcaggcg tggcccaaaa gcataactgt ccccttgtcc ggctggacta
    19501 ccagcaggaa cagggccttg caagcagcct gccgctgggc gtcaatcaga tcaagataca
    19561 gcgcagcctt acgacttcca gcgtggccgt gttcgtcccc tttgtgacgc aggagctttt
    19621 tcagggcggc gcggccatgt attacggcat caacgccaag tcccgcaaca tgattatgct
    19681 ggacaggaag caggcccgtt gccccaatgc cttgaagctc ggcacccccg gcagcggcaa
    19741 atccatgagc tgtaaatccg agatcgtcag tgtgttctta acgacgcctg acgatatttt
    19801 tatttcagac ccggaggccg aatattaccc ccttgtcaaa cggctccacg gacaggtaat
    19861 ccggctgtcg cctaccagca aggactttgt gaacccgctg gacatcaacc tgaattattc
    19921 ggaggacgat aacccgctgg ccctgaaatc cgactttgtg ctgtcgttct gtgagcttgt
    19981 catgggcggc aagaacgggc tggaagccat tgaaaagacc gtgatagacc gggctgtgcg
    20041 cgtgatctac cggccctatc tggcagaccc ccgcccggag aatatgccga tcctgtccga
    20101 cctccacaaa gcgttgttag atcagcatgt cccggaggct gaccgggtgg cgcaggccct
    20161 tgacctgtat gtgtccggct ccctgaacgt atttaaccac cgtaccaacg tggacatcgg
    20221 caaccggctt gtgtcctttg acattaagga gctgggcaag cagctcaaaa agctggggat
    20281 gctcattgtc caagaccaga tatgggggcg cgtcacggcc aaccgaagcc aaggaaaggc
    20341 cacatggtat tttgccgacg agttccattt gcttttgaaa gaggaacaga cggcggccta
    20401 ttctgcggag atttggaagc ggtttaggaa atggggcggc attccaacag gtgccaccca
    20461 gaacgtcaaa gacctgcttt cctccccgga aattgaaaat atccttgaaa acagcgactt
    20521 cattacgctg ctcaatcagg cgtccgggga tagaaaaatc cttgcggaac gcttgaattt
    20581 gtccaccgaa cagcagaagt acatcgacaa ttccgaaccc ggcgagggcc ttttgatctt
    20641 tgaaaacgtg gtgctgccct ttaccaaccc catcccgcac aacacccagc tttacaagat
    20701 tatgacgacc cgcttaaacg aggtggcggg agtatgaccc ggcgacgcca caaaggaggt
    20761 gaaccaccat gaaagagctg aaagccaagg cgaaagtgac gcaggccatg acccgcgacg
    20821 ggctgacgct ggaaaatcag gccaccggcg aggtggagaa catttccagc cgggaggccg
    20881 aacaggattt gtccgcgccg gggccgggcg gcaccgccga taagctgctg gaacgggccg
    20941 acgccatcca tgacggccat aaggccaaga aagccgcgaa gcaggccggg gctgtggccg
    21001 ccgaggggga cgctgccctg caccggccat ccgcccggct ccaactgtcg gaggaagaac
    21061 gggccgaccc ggatttacag ccctatatcc gaaaatcgga caaacgggcc gataagctgg
    21121 ataaggcccg cgccgccatc cccaagaagc gcgtcccggt caaggaaacc gtctttgacg
    21181 aggcaagcgg caaagcaaag accgtcctgc actttgacaa acaggacaag gggcctccct
    21241 ccctgaaacc caatccggca ggccgtcctg tttccgaggt ttttctattc ggccacggga
    21301 aaatccatga agtcgagcat gaaaacgtgg gtgtggaggg cggccataag ggggaggaac
    21361 ttgccgagcg gcaggccgga aaagccatcc gcagcaccat ccggcaccac aggctgaaac
    21421 cctactggga cgcggaaaag gccgagcgca ggctattgtc tgccaacgcg gagtatttct
    21481 accagaaatc tttgcgggat aacccggaga tcgcacaggc ggccagcaac cccatttccc
    21541 ggttctggca gaaacagcag atcaagcgcc agtacgccaa ggccgcccgg acagcgggcc
    21601 agaccgcagg cgcggcggca cagggcgcgg ccaagacagc cggggcctcc acgaaagcgg
    21661 ccaaggccgc agccaaagaa agtgaacggg cggcctcctt tgtggcccgc cactggaaag
    21721 gggcgctggc cgtccttgtg ttggggcttt tgatgctgct tatcatcggc ggcttacagt
    21781 cctgcaccgc catgttcggc agcgccggaa gcggcatcac ggcgtcctct tacttttccg
    21841 aggatagcga tatgttggcc gccgaggacg cctacgcgga gctggaagcc gagctgcaag
    21901 actatctgga caactacgag gccacccatg actatgacga ataccatttt gaccttgacg
    21961 agatcagcca tgacccctat gtgctgattt ccattctgtc ggcgctccat gacgggatat
    22021 ttaccatcgg ggaggtacag ggtgatttgt ccatgctctt tgacaagcaa tatatcctga
    22081 cagaaaacgt cgtggtagaa acccgttacc gcaccgaaac cgatacatgg acggacgcgg
    22141 agggcaacac ccatacagac acctaccaag tgccgtatga ctattacatc tgctacgtca
    22201 cgctggaaaa cttcgacctg tcccatgtgc cagtctatat catggacgag gaaacgctaa
    22261 gtttctattc cctgtatatg tccacgctag gaaaccggcc tgatctgttt tccggcagcc
    22321 agtaccccaa cgcctccacc ctgacgccgc cgacctacta cgacgtaccc ccggaggctt
    22381 tggaggatgc cacctttgcc gccatgctca aagaggcgga aaaatatatc ggttatccct
    22441 atgtgtgggg cggcagctct cccagcacca gctttgattg cagcggcttc atttcgtggg
    22501 taatcaatca tagtggatgg aacgtgggac ggctgggggc gcagggccta tataacatct
    22561 gcacccctgt ttccagttcg caggccaagc ccggcgacct tgtatttttc aagggaacct
    22621 acgacacccc cggcgtgtcc cacgtcggcc tgtatgtggg caacaatgtg atgctgcatt
    22681 gcgggaaccc gatttcctac acgaacctta cttcaaacta ctggcaatcc catttttaca
    22741 gttacgggcg tttaccttaa cgccagaaag gagctttcaa ttatggcaac caaacttgac
    22801 cgcatcgaaa aggacatcca gaaaaccaag tctaagatcg cggagtttca gaaacagctt
    22861 cgggagctgg aaacgcagaa aaccgaacag gagaatttgc agatcatcca gcttgtgcgc
    22921 ggcatgaaca tgaagccgga ggaatttgcc gcgttcttac gcagcggcgc aatgcaggcc
    22981 gcccctgccg caacccctta ccatgaacag gaggacaatg cccatgaaga ataagaccag
    23041 acggatttcc cgtattctcc ccgcacttct ggccgtgatg ctctgcgtca cggcctttcc
    23101 cgttaccgcc cttgcaggcg gcaatgaccc ggcaccggcc ccgctgccgg aggctacaac
    23161 cgaacccgct acgggcggtt tggagcctga aacagacgaa accggcaccg ccacggacgg
    23221 gacagaaacc gaggccgaaa aagataccgg ccctctgacc ggcaaggaca tcaccgacct
    23281 gttttccgcg ctgttcggtt ccaaggtgag cattgccgcc acggacgacg gcatccagat
    23341 caccaccggc accgacaaaa aagaacctga acagaccggc accgtcacca ccaacggcgg
    23401 caggctgaat gtccgcaccg gcgcggggct tgacaagacc gcgttcaccc agcttcccaa
    23461 tgggacgacg gttgaggtag tcggcacaga cggcgactgg attaaaattc tcttgccgga
    23521 gcgcatcggc tatgtccatt ccgactatat gaccgtcagc gaaaaggaag tagcggctac
    23581 cggcgagggc ggtttttccc tctccattga cccggaggaa attgcttctc tgctggaact
    23641 gttcaatggc ggcggggcca gcgccgccct gtccccggac gggaacctgt cactgattga
    23701 cgacatcggc agcgccgcga aaaatggcaa gcagtttatc accgtggaaa ccaaaaacgg
    23761 caatgtgttt tatctgatta ttgaccggga cgacgaggga aaagaaactg tccatttctt
    23821 aaatcaggtg gatgaagccg atctgctgac gctgatggag gacggggaaa ccgtcacccc
    23881 cgctgctgtc tgctcctgta ccacgaaatg caaggccggg gctgtgaaca cgaattgccc
    23941 ggtatgtaaa accaacctga ccgagtgcag cggcccggag ccgcaggaac cccagccgga
    24001 ggaacccgaa gccccggagg aagaacccaa gggcggcgcg ggcggcctga tcgtgttcct
    24061 gctggtggcg ctggcgggcg gcggtgcggc cctttactat ttcaagttca aaaagcccaa
    24121 ggctgaaacc accggcagcg acgatttggg cgagtatgat tttggggagg acgaggattt
    24181 ggaggacgag gaacccgaaa ccgagtttga cccggaagct gattttgtga gtgaacagga
    24241 ggacgaggaa tgacatataa acttgtaatc gcggaaaaac ccagcgtggc ccgcagcatt
    24301 gcgggcgtga tcggggccac ggagaaacac gacggctatt tggaggggaa cggctatctg
    24361 gtgagctggt gcatcggaca tctggtgtcc tttgccgacg ccggacgcta tgacgagcgt
    24421 ttcaaaaaat ggcgctatga ggatttgccc attctcccgg aaacatggca gtacatcgtc
    24481 cccgacgaca agaaacagca gtttgacgtt ctctgttcgc tgatgcagcg ccccgacgtg
    24541 accggccttg tgtgcgccac cgacgccggg cgcgaggggg aattgatttt ccgtttcgtc
    24601 taccagatgg cgggatgcca aaagcccttt gagcgccttt ggatttcctc tatggaggac
    24661 agtgctatta aggacggctt tgcccacctg aaaccgggcg gcgactatga tccgctgtat
    24721 caatcggccc tgtgccgggc caaggccgac tggctgatcg gcattaacgc cacccggctt
    24781 ttttccgtcc tgtaccacaa gaacctgact gtgggccgcg tccagacgcc gaccttaaaa
    24841 atgctggtgg acagggacgc caagattacc gatttcaaga aagagaaata tcacatcgtc
    24901 catatcacgg cgggcggcgc ggacgcggta agcagccgct tttccgacgc tgccgaggca
    24961 aacaccgtca aggcggcttg tgcaaaagcg caggccgttt gcgtttccgt gacgcgggag
    25021 aaaaagacgg aacagccgcc caagctctat gaccttacca ccttgcagcg ggaggctaac
    25081 cggcttttcg gctataccgc caagcagacc cttgactatg cccaggcact gtatctaaag
    25141 aagctgctga cctatccgag aacggatagt caatatctta ccgacgatat gctgcccgcc
    25201 gtggaaagcc ttgtgtccgg cctgtgggag ctggtgccgt ttgccaaggg cctgaacctt
    25261 tccccgcagt ttgaccgcat cctgaacagc aaaaaggtgt ccgaccacca cgccattatc
    25321 cccaccgctg aatttgtgaa gcagggcttt ggcactctgg ccgagagtga aaaaaagctg
    25381 ctgtccctga tctgctgcaa gctcttatgt gccgtggccg ggccgcatat ctatgaggcc
    25441 gtcaccgcca catttacctg tgccgggcag gagtttaccg ccaagggcaa gaccgtcctt
    25501 tcccccggct ggaaagagat tgaacgccgg ttccgttctt ccttaaagac cgacgccgac
    25561 gaggacgccg aggcggtgcg ggagctgccg gagctttgcg agggccagac ctttgcagac
    25621 gtggccgcct ctgtcaccga gcatttcacc acgccgccca agccctacac cgaagatacg
    25681 cttttgtccg ctatggaacg ggccggggcc gaggatatgc cggaggatgc cgagcgcaag
    25741 ggattaggca caccggccac ccgcgccgcc attctggaaa agctggtgca gatgggcttt
    25801 gtccagagaa aaggcaagca gctcattccc accaaggacg gcatcaatct ggctgtggtg
    25861 ctgccggagg cgcttacttc tccccagctt actgccgaat gggaaaaccg gctgaccgag
    25921 atcgccaagg ggcaggccga tcctgacgaa tttatggacg gcatcgagac gcaggcgcgg
    25981 gagctggtgc agacctattc ctgtatcagt gaggaaaagc agaaattgtt tcaggccgag
    26041 cgtgttccta tcgggacgtg tccccgctgt ggtgaggctg tctatgaggg caagaaaaac
    26101 tactactgcg gaaaccgggc ctgccagttc gtcatgtgga agaatgaccg attttttgag
    26161 gaacgcaaga aagcgttcac cccgaagatc gccgctgcgc tgctcaaaac cggcaaagtc
    26221 aaggtaaagg gcctctattc cgtaaagacc ggcaaaacct acgacgggac ggtgcttttg
    26281 gctgataccg ggggcaagta cgtcaactac cgcattgaac acaggagcta aaggacagta
    26341 acacagcaag catagggagt gtggctacac tcccggtaaa atcccccggc cgcgtccctg
    26401 ccgggccggg gatttttgct tgttggggaa gtccccaacc ccctattttt tgaaacagaa
    26461 cagagaaagg agtgaccgcg tgaacgaagt attttctatc caagtccata accggcagtt
    26521 tttggaacag ggcaaacaag gcgtctggct ggatttgccc accacaaccg aaacgctgca
    26581 agccgccctg cgggatgttg gcattactgc cgacaatccg caggattttt ttatcaacgg
    26641 gtattcctgc ccggaggaca gacaccttgc cattccctat gacatggtgc tggccgccga
    26701 cgtggacgag ctgaatttcc ttgccgcccg gctgggccag cttgacgccg ccgagatcgc
    26761 agagctgaac gccgccctgc aaaatccccg tggcggcttt gagaatatcg gccagatcat
    26821 cgactacacc gaaaatgtgg attactatgt gcatcttccc gacgttaggg gggatggaca
    26881 gttgggcgac tactacctga accgttccgg catggtggat atgccggagg aatggaaagc
    26941 cgggattgat acggcgcagt ttggacgcca cattgcccag caggaacaag gcgtttttac
    27001 ccagtacggc tatctggtga gaagcggcga cgaatggcag cgcgtccatg agggccagcc
    27061 ggtgccggag gaataccggg tgatgcacta cccccagccg gaagctgacc gggaggaacc
    27121 gcaggcccag cagccggagg catccgaaag ccccgacaaa gcgccgcccc cggttatccc
    27181 gatcatgctg gacggcatga acagcaaaga acgcatgaaa gtgattactg accgtctgga
    27241 aaccggcgta caggagcttt ttgaaagtag ccgctatatg gcttacttat ccgctatgtc
    27301 caagatgcac gattacagtc tgaacaatac gctgctgatc gccatgcagg gcggttcgat
    27361 tgtggcgggc tataacaggt ggcaggatga atttgaccgc catgtgaagc ggggcgagaa
    27421 aggcattaag atactggccc cggccccgtt caaggttaag cagcagatgg agaaaatcga
    27481 cccggccacc cagcagcccg tgatcggcgc ggacggcaag cccgtgaccg aagaacagga
    27541 agtgacggtt ccggccttta aggtggtgac agtctttgac atcagccaga ccgagggcaa
    27601 ggattttccc atgccctata tcagtgaact taccggcgac gtgcagcagt atcaggattt
    27661 ctttgccgcg ctggaacgga catcacccta tcccataagc attgagccga tttcagacgg
    27721 ggccaagggc cgctgcttct acgcaaaaca gcgcattgcc atcaacgagg gcatgagcga
    27781 attgcagacc ctaaagaccg ccatccatga gattgcccat gccaagctcc acgccattga
    27841 ccgggaggcc cccgccgagg aacaggcggc ccggccagac cgcagcaccc gcgaggtaca
    27901 ggccgaaagc gtggcctata ccgtctgcca gtattacggg ctggacacgt cggactattc
    27961 ttttggctat gtggccgatt ggagcagcgg cagggagtta tccgagctga aagcctccct
    28021 tgaaaccatc cgggccacgg ccagcgaatt gataaccgag atcgacggcc attttgccca
    28081 gcttcagcag gagcgcgagg cccggcagga acaggaggcc gtcacggttg acgttacccc
    28141 ggaggcagcg gccccggcgc aggccgagga agaacaggcc ccggcccttg atccggcagc
    28201 cgagccgctt gtgactgtgc tatggagcga aagcccccag ctaaaggacg gccagcaaat
    28261 gccgttgcat gaggccgacg cgctgtttgc ccgtctggat gcagcatatc cggcagaggc
    28321 aggctatgac aaaaccgcgt tccgcattga ctgtatcttt cagggcgagc cggacagcta
    28381 cgaggggcgg caggattttg gagataggga cggttccctg atcgaacata tccagagcta
    28441 ccaccaattt tacgcgcagg acgaaaactg gaaaaagcat gtaatcgcta caaccggcat
    28501 agagggctgg gagcaggaca aggcccatcg ggatatgctg ttaggcgagt ttgtccccta
    28561 tatgaagctg cattgcagcc ttgcccaaat ggaacagacg gcgcagggcc gcttacagtc
    28621 cggggaaacc ctgacgccgg aggaaacggc ctatttgggg gcgcttctct cttatgtgca
    28681 ggagtgccgc cccatgctca atcagggcca gtatcagttg ccggagccgc cgaaactggc
    28741 tgacttcgac cagagcttgc aggactacaa agcacaggta caggccgaga tcgcgcagga
    28801 ggccgccgca gccggtatga ccgtggagga atacgcggcg gctggctatg aagcacccca
    28861 acaggacacc ttttccatct accagcttcg aggcggggag gaaacccgcg actatcggtt
    28921 tgagccatac gagcgtttac aggccgcagg gctggccgtg gacagtgcca actatgacct
    28981 tgtttatacg gcaccgctgg ataaaagcac gtctttggag gatatttacc gcacattcaa
    29041 catcgaccac ccggcagatt ttaggggaca cagcctttcc gtttccgacg tggtggtgct
    29101 gcaccagaat ggacaggaca ccgcccacta tgtagacagc ttcggctata tggaagtgcc
    29161 ggagtttttg cgggaaaatc ccctgcgtac cgccgaactg tctacggagc agaacgaaaa
    29221 catgattgac ggcattatca acaatacgcc gcccgaaccg cagccggagg aacccgaaca
    29281 gctatctttt gaggatgtgg cagttgcttc cggccagaca caggaggaac ccccggaggc
    29341 tgcgcccccg caaacggctg tccgctatta tcccatcaat gagggagccg cccgccgcgc
    29401 caaggaagcc atgagctttt ctgactaccg gcccggcagc gccacggcgg aataccggca
    29461 ctatgtcgat caggccgtgg aaattgcgga gcgccagaaa aagcgtgtgg agccagagta
    29521 tcacgaaaag atagacagcc tccttgacac ctacgcccgc aagctggccg agaacatgaa
    29581 taagggctat gagattaccg cccgcgtccc ctctgtcctg attgccgggc cgtccaattt
    29641 cccggtacgg gcgaaagaaa agcagaacgc cgccagtgac cgcaacatgg aggaatttca
    29701 gtacattcag ggcttgcttg acaaaatccg cagcaccggc atgggtggta tcagcgccga
    29761 cgatccgcag gccgtttcca agctggaaaa gaaactggaa aagctggaag cgtcgcagga
    29821 gttgatgaag gccgtcaatg cctactaccg caagcatggg acgctggacg gatgccccca
    29881 tctgaccgaa agaggcattg aaaacctgaa agcggatatg gcgtcgggct ggcactatga
    29941 gaaaaaaccg ttccagtcct ggcagctctc caacaataac gccgagatac gccggttaaa
    30001 gggccgcatt gaggaattga cccgccaaaa ggaggcggcc tatgtgggat gggagtttga
    30061 cggcggcact gtggagatca accgggtggc caaccgcctg caaatcttct ttgaggggaa
    30121 gccggatgct gctgtacgcg acgagctgaa aagcaacggt ttccgctggt cgcccaaggc
    30181 cggggcatgg cagcggcagt taaacgacac cacaatccgc gtcgctgacc gtatcaagtg
    30241 catccagccc ctttccggcg aaaagcccag cgccttacag cttgcggccc gcagggagaa
    30301 agaaaagccc tctatccggg cgcagctcaa cgcggccaag accgaacagg ccaagcagcc
    30361 accggcgcgg gaaaaatcaa agggattgga ggtagagtaa atgcagaagt ttgaaaatgt
    30421 ggacgttctg gccgcgctgg aacagatcat gcggcagaac accgcgtttt atcaaagtga
    30481 ctttggcatt gacaaagaca ttatcaggcg ggatgccgcc agcgatcagg ccgcagacaa
    30541 gaccctgctc tggatgtccc ggccctccgg gacgtactgt ttccgggaac gtgatgtttt
    30601 cctgaaagac acccgacagt acaacacatg gaaattttac ggggagcaga cccgtgacaa
    30661 gatactggcc tacgcggtgg agctgaccgg cacacagggc ggcactatcc ggggcaatct
    30721 ctatgagctg gactatccgc agcatttccg gcacgtcatt gaaaccgccc agccggtgag
    30781 tgtgaaccgg ctcttttatg agcatggcac ccgtgacatc ccggagagcc agcgttttga
    30841 cggaagccct gaccgggtat tgggaaactt tttgtactat gaggcccagc cccatgaccc
    30901 ggctgtgctg caggaggcgc tacggacgga gcagcacagc cgggggcggg ccgtccccgg
    30961 cgatttcaaa gtccacgtca ccgctttgca tgacagccgg attgaaaccg aggcccggcg
    31021 tattgtggac agcctgaaag atctgtccgc gcctaacagc cccaataaaa cgcatttcat
    31081 ggtggagatc tccccgcact ttgcggcgat tgcttccacc aaggataatg accggctctt
    31141 tgccatgctg ccctataaat ccctgtgttt taccgggatg aaaggcaggc atggcgtgta
    31201 tgccgtgatt aaccgggacg agcgccggga tgtgagtatt cgcaggcccc ggccctctat
    31261 ccgcaagcag ctatccgaca gcaaacaggc cgccagccca aaaaaggcgg cggccagaac
    31321 caaaaagaat gaattggagg tataaggaat gatgcacttt accgtggagg aagaaaacct
    31381 gatctgtatg taccacaatg cagaccggcg cagaacgata aacaggctca ccgccgtcat
    31441 gccggttatg gatggggata tgcgggcgca ggcccagcag accctttcca agctggaacg
    31501 catgacggat gccgactatg acggccagaa gttccacttc acagacgaat aacgaacagc
    31561 agacgccggg cgcggcggcc atgtggtcgc ttgcgtccgg cgtcttttct ttttgcctat
    31621 tcctacgcat ttagaacccg gtattgtcgg ggataggatg aagtattcgc ttgcccctgc
    31681 aaacatgcgg gagaagcctt gcagcgggcg gttttcaatc ccttaaatgc gtaggttgca
    31741 gctataatta gacccctgaa acagatatag ccgcgcagcg ggtgtatctg tttcaggggt
    31801 gtgcaggtga tagccgcaaa gcggcgtaag agggtgcagc cctcttgccg tctatctctg
    31861 ttccagcggg aagctcggca atgcttccag cagtttcagg gaaatgcgct gccgtatatc
    31921 ttcgtcaatg actttcttct ttttgccgtt tgcctgtctg atctccaccg tggaaagctc
    31981 gttgatgtag tcagcataat gggccaacac tttttccgtg gcccacctct caccggcaac
    32041 ggcggctttg atcgtttcat agtccagcag cttttcttgt ccgtccatcc tgataccccc
    32101 gtcagactga ttttttctat gataccatat tcggaagcat ggaaccagag aaaacggcgc
    32161 ttttctgctg gtaaattcta cgcatttaga acagcacttc ggcggggata ggataaaaca
    32221 cccacatacc ccgctgacca tgccggaaaa gccttgaaat ataagggctt ttacagctct
    32281 aagtgcgtag gtttccagta tcttttccgc tggcgctcgg cctctttgcg gcggcgcatc
    32341 cgtttggagc aggccgcgca gtatttggcc cggttagact tcggcgtgaa cgtggccccg
    32401 cactcacagc agcgtttcac ttcgtcccgg tctttggtga tctcggcata cagggccgca
    32461 tccagcggga ggacagcggc cttaaaccag cggcacagaa gcgaatagga aatgctctgt
    32521 acgcaaatac attcttcgcc gtcgtccagc aacaggcagt tgccgtcaca atagttgcag
    32581 cactcatgcg tcagcttccg ggccttgcga tattgagggt aattcattcg tggaatactc
    32641 atagctcttg cgtccattcc tttcccggcg tcatacgcaa aatgccgtcc acattctgct
    32701 tgatgatgcc gtattcctgc atctgctttt tcgcttcgcc gtacttctca tacagccgtt
    32761 ccttttcctc ggccaatcgc ttgtactcgg ctttcagggc gtacagatcg gggagctttt
    32821 tcacaccctg tccctttagg gcctttgccg cagcttcata caaaatcaac gtgctttcat
    32881 gctcacgccg gaattttgcc ttatccgctg cattgcggta ttccatagcc acgggccaga
    32941 gctgcttgta ggtagaaata ttctttatca gcaccgccat atccgcaagc cggtgttcca
    33001 tagctttgag ggtagcggcg gcctcgtcgc tggcggcggt gatctcggcc actttgcttt
    33061 ccaaatccgg gtaggtgtct atcccatgtt cagtcaggaa attcaaagtc tttgcggcct
    33121 gcttcaaatt gtggagcttc gcccacctct catagcctgc cgactgctgg gccttaatgt
    33181 tgttttccag atcaatccgc aaagaaatcc ccttgcgctc tttgggcgct ctggtgataa
    33241 cgcggccttt gatacgctct ttgatggctt cctcggtgta ggcttccccc aatgtcttgc
    33301 agcgggtaaa gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc ggcttgatct
    33361 catagccctg cgcctgcatg agccgcagga aatcatcaaa atcttttgcc tgcgggatag
    33421 cggcgtcaat ggctgctttg agcttcgctt tccagctcgt ccctttccgc tgggcgtccc
    33481 actcggcata gctcttgccc ttactctggc cgggcttcac cacggacaat ccgttttcct
    33541 tgcacagcct gtcgctggtg cgccggatat aggcatagct ctgtttgttg gaaatgtact
    33601 tgcgctgatt taccatatcc acggcacaga aaataatgtg attgtggacg tggcccttat
    33661 caatatgggt agtcagcaca tagggatatt tcccttgcag cacttcctcg gcaagctgct
    33721 tcccgatctc atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat tgtatcaggt
    33781 ggtggcccag attattgccc ttttggtatg cctgcgccag cagcatttca aattcaatgt
    33841 ccgctgtttc ataagagcag gcaaaggagg ccaccagcag cttgccgtca gttttgtccg
    33901 ggttctcgat gtagtcaagg gcttttttca gcgttgactt gataggatgt atctttgtaa
    33961 ctgccatatc tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt acaccggccc
    34021 cataccgttc agcctctttg caatctgatt gacgttgacg ccgatcttct gtatctcggc
    34081 ggtctgcgcc cggatgtcgc tggtgtccat gttgacgata tagccgtcaa tgagcatctt
    34141 ccgggcgtag gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt caatcatagc
    34201 ccgttcctcc ggcgtcacag ggacgcgcag cacgattttt cgctttctgt ttgccatgct
    34261 tcaccgtcct ttccattcag gggtttgggg atttccccaa caagcaaaac ggacgcggcc
    34321 cggcagggac ggggcaggcc gtttttccgg gagtggctac actccctatg cttgctacgt
    34381 tactgtttct ccactaacaa gacgtttcca aagccccaaa ctacccgcaa aatagccctc
    34441 tggaaagaaa aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc taaaagtaaa
    34501 acagaggatg ccagttacac atcctccgtt tcattcttct tcggcttttc aattagtatg
    34561 gcgctgtcaa tcgcgccctc gatgatcggc aggtattctt cggggcagag tttcagcttg
    34621 tggctgaccc tttgccgcag ttcgctttcc tcccgcatga tctccgggtt aaagtagcgt
    34681 tccacaggaa gcccgcaggc tttaataagc tgtatgacaa ccggcagact gggaatggcc
    34741 ccgtcgtttt cgatgttggc aagatagcgc cagtcaatat ttattttctc tgccattgct
    34801 ttgcgcgaaa cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag
    34861 ccgggacaat cttcaatatt cgccatatcg cacctcccac ttacattgta tatttcatac
    34921 tctgcccgtg gaatgttggt atatgcacct ttcttaaagt ttatatttca cattttgttt
    34981 cttttattct tcgagcaaaa gaattataat gtaccctgtg gaggtgtact atggactaca
    35041 tgacgttgaa agaggcaggc gagaaatggg gtgtgccccc acgatggata aactactatt
    35101 gtgctgcggg acgcatcccc ggcgctgtaa agatggcgac gatctggctc attcccaaaa
    35161 ctgcggaaaa gccgattgac aggcgtacaa agaaaggaaa ggcggttact gaatagtata
    35221 atgaaaccgg cgcattttgg atatggtttt gaagagagag aaagcatggt attttgtcac
    35281 ggaaaaacta taagatgata gatggcaggc tgctccagac cgataaaaag tattctcatc
    35341 tgaaacagaa agaaaagatt tacacatgga tgtatgaaga agcgaaactt tatcatgaca
    35401 gaaatggaaa atgcccgaaa caaagacgag gaagctgctg ctgtatcagc cgtatatggt
    35461 agaattgaaa aggccggcat ctggattctt tatggtgaag tctccagaca ttggataact
    35521 gctggtctaa tgcgcctttg agtatggggt gattgatttt aataccgggg aggtacggct
    35581 ttttgaggaa aatattctgg gatttacatt tggcgattat tacatttcct gtaactgcaa
    35641 ggacacggat gatgaagata tgaggaatat agacttgctg attttctact gtcttgaacg
    35701 gtattaggac tgaatctgct gcgcggagtg cggcaagagc gaccggaggg agtacacaca
    35761 ggccctgcct gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat
    35821 tttgctgagg cttggggtgt ccccaacaag caaaacgaga acgccgcagg cgggcaagtg
    35881 gtggatcaga atatccgtaa aggatattct gatccattgc ttgccagtat tatcgggatg
    35941 ttttgacaac cgctgtaatc gaaacatccc aaacattaag aaggtgttaa ttattattat
    36001 cttgatttaa ttcgtttaaa ccaataatat agatacatac accatgaaaa gaagcgagta
    36061 tcccaatgac ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca gggaaactac
    36121 agactgttat gttatcttct taaaatggag ggatttttat gtcgatacga attctacttg
    36181 tcgaggatga tgatcatatc tgtaatacag taagggcgtt tctggctgag gcaggatatc
    36241 aggtggatgc ctgcacagat ggaaatgagg catacaccaa gttttacgaa aacacttatc
    36301 aactggttat tcttgatatt atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat
    36361 ttcgtgcgaa aaatgatact cccattctga tgatgacagc cctgtcggat gacgaaaacc
    36421 aaatccgggc gtttgatgca gaggcagacg actatgtaac aaagccattc aagatgcaga
    36481 ttttactaaa gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc
    36541 gtgtcggcag gctgacactt ctgccggagg atttcacggt actttgtgac ggtacagagc
    36601 tgcccctgac acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa
    36661 ccttaacgca tgaaatcatt ttgtcacgta tatggggata tgactttgag ggtgatggca
    36721 gcacagtcca cactcatatc aaaaatttgc gggccaagct gccggaaaat atcatcaaaa
    36781 ccattcgcgg tgtaggatac cgattggagg aatcataata atggaaagaa aagggatttt
    36841 cattaaggtt ttttcctata cgatcattgt cctgctactg gttgtcggtg taacagcaac
    36901 actgtttgca cagcaatttg tgtcttattt cagagtgatg gaattacagc aaacagtaaa
    36961 atcctatcag ccattggtgg aactgattca gaatagcgat aggcttgata ttcaagaggt
    37021 ggcagggctg tttcactaca ataaccaatc ctttgagttt tatattgaag ataaagaggg
    37081 aagcgtactc tatgccacac caaatgccaa tacatcaaat agttttagac ccgactttct
    37141 ttatgtggta catagagatg ataatatttc gattgttgct caaaacaagg caggtgtggg
    37201 attgctttat caagggctga caattcgggg aattgttatg attgcgataa tggttgtatt
    37261 cagcctttta tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca aagccttagc
    37321 ggacagtgcg aataaaatgg caaacctgaa agatgtaccg ccgccgctgg agcgaaagga
    37381 tgagcttggc gcactggctc atgatatgca ttccatgtat gtcaggctga aagaaaccat
    37441 cgcaaggctg gaggatgaaa tcgcaaggga acatgagttg gaggaaacac agcgatattt
    37501 ctttgcggca gcttctcatg agctaaaaac gcccatcgcg gctacaagcg ttctgttgga
    37561 gggaatgctt ggaaatatcg gtgactacaa agatcattct aagtatctgc gcgaatgcat
    37621 caaaatgatg gataggcagg gcaaaatcat ttccgaaata ctggagcctg tcagcctgaa
    37681 tgatgggaga atcgtaccca tagctgaacc gttggacata gggcgcacgg ttgccgagtt
    37741 gctgcccgat tttcaaacct tggcagaggc aaacaaccag cggttcgtca cagatattcc
    37801 agccgggcaa attgtcctgt ccgatccgag gctgctccaa aaggcactat ccaatgtcat
    37861 attgaatgca gttcagaaca cgccgcaggg aggcgaggta cggatatgga gtgagcctgg
    37921 tgctgaaaaa tgccgccttt ttgttttgaa catgggcgtt cacattgatg atactgcgct
    37981 tccaaggctg ttcaccccat tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg
    38041 aagcggttta ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc
    38101 gctggaaaac acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt
    38161 gtaaatattt aaaacttaaa tgattttgac cgacaggtat aaccctgccg gtcttttttt
    38221 gctgctacag gaaaactggc tctttgtcaa taaggactga tgagttgtgc aaaatc
//
LOCUS       DAGCXG010000292        16763 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.048, whole genome shotgun sequence.
ACCESSION   DAGCXG010000292 DAGCXG010000000
VERSION     DAGCXG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.048"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            142..948
                     /locus_tag="MB022_002951"
     CDS             142..948
                     /locus_tag="MB022_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBT4467861.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            966..1793
                     /gene="vanW"
                     /locus_tag="MB022_002952"
     CDS             966..1793
                     /gene="vanW"
                     /locus_tag="MB022_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HBT4467862.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1790..2761
                     /locus_tag="MB022_002953"
     CDS             1790..2761
                     /locus_tag="MB022_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4467863.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2754..3782
                     /gene="vanB"
                     /locus_tag="MB022_002954"
     CDS             2754..3782
                     /gene="vanB"
                     /locus_tag="MB022_002954"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4467864.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            3788..4396
                     /locus_tag="MB022_002955"
     CDS             3788..4396
                     /locus_tag="MB022_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4467865.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4462..4635)
                     /locus_tag="MB022_002956"
     CDS             complement(4462..4635)
                     /locus_tag="MB022_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467866.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            4983..5414
                     /locus_tag="MB022_002957"
     CDS             4983..5414
                     /locus_tag="MB022_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4467867.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            5421..5654
                     /locus_tag="MB022_002958"
     CDS             5421..5654
                     /locus_tag="MB022_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467868.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            6078..6278
                     /locus_tag="MB022_002959"
     CDS             6078..6278
                     /locus_tag="MB022_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBT4467869.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            6362..7555
                     /locus_tag="MB022_002960"
     CDS             6362..7555
                     /locus_tag="MB022_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4467870.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            7733..7963
                     /locus_tag="MB022_002961"
                     /pseudo
     CDS             7733..7963
                     /locus_tag="MB022_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            8091..8774
                     /locus_tag="MB022_002962"
     CDS             8091..8774
                     /locus_tag="MB022_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBT4467871.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            9083..9156
                     /locus_tag="MB022_002963"
     tRNA            9083..9156
                     /locus_tag="MB022_002963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9117..9119,aa:Arg,seq:tct)
     gene            9315..9674
                     /locus_tag="MB022_002964"
     CDS             9315..9674
                     /locus_tag="MB022_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBT4467872.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            9797..11428
                     /locus_tag="MB022_002965"
     CDS             9797..11428
                     /locus_tag="MB022_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBT4467873.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(11490..12131)
                     /locus_tag="MB022_002966"
     CDS             complement(11490..12131)
                     /locus_tag="MB022_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4467874.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    12317..12393
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            12413..12721
                     /gene="rplU"
                     /locus_tag="MB022_002967"
     CDS             12413..12721
                     /gene="rplU"
                     /locus_tag="MB022_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBT4467875.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            12748..13092
                     /locus_tag="MB022_002968"
     CDS             12748..13092
                     /locus_tag="MB022_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBT4467876.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            13105..13398
                     /gene="rpmA"
                     /locus_tag="MB022_002969"
     CDS             13105..13398
                     /gene="rpmA"
                     /locus_tag="MB022_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBT4467877.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(13491..14639)
                     /locus_tag="MB022_002970"
     CDS             complement(13491..14639)
                     /locus_tag="MB022_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4467878.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(14651..14962)
                     /locus_tag="MB022_002971"
     CDS             complement(14651..14962)
                     /locus_tag="MB022_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467879.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(15015..15410)
                     /locus_tag="MB022_002972"
     CDS             complement(15015..15410)
                     /locus_tag="MB022_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBT4467880.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(15446..15790)
                     /locus_tag="MB022_002973"
     CDS             complement(15446..15790)
                     /locus_tag="MB022_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4467881.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            16091..16312
                     /locus_tag="MB022_002974"
     CDS             16091..16312
                     /locus_tag="MB022_002974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4467882.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            16439..>16763
                     /locus_tag="MB022_002975"
     CDS             16439..>16763
                     /locus_tag="MB022_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBT4467883.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYL"
BASE COUNT         4936 a         3200 c         3790 g         4837 t
ORIGIN      
        1 caattctgat tcatcagtcc cataaattat agagccggaa aactacagac tggctacagg
       61 gaaagtacag atgcgcttgc cataataaca atcgtaccag ccacaaatcg tagttttatt
      121 gcaaaggagg cattcgatca aatggaaaaa agcaactatc attccaatgc ggatcatcac
      181 aaacggcata tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg
      241 ttcacagtct gcacgctgtt tttggggtgg agactggctt ccgtattgga ggcaacacag
      301 ataccgccca tccctgcaac tcatacaggc agcagcactg acgtagtgga gaatttggag
      361 gaaaacgctc ttgccaccgc caaagaacag ggagatgaac aggaatggag cctgatttta
      421 gtgaacaggc agaaccccat ccccgcacag tacgatgtgg agcttgagca actatcaaat
      481 ggtgagcgga tagatattcg gatttctccc tatcttcaag atttgtttga tgccgcaaga
      541 actgatggag tttacccgat tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa
      601 attatggatg aaaaaattgc cgaatataag gcgaaaggct acacctctgc acaggctaaa
      661 gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg agcatcagct tggtcttgct
      721 gtggatatca atgcggacgg aattcattca acaggcaacg aggtttatag atggctggat
      781 gaaaacagct atcgttttgg ttttattcgt cgctacccac cagacaagac agagataacc
      841 ggtgtgagca acgagccgtg gcattaccga tatgttggca tcgaagctgc cacagagatg
      901 tacaaccaag gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt
      961 atgttatgga cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca atgagacggg
     1021 cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga
     1081 cgatagggga aaaatcgctt tcccatttgc tctttgaaac ggattgcgca ttatacaacc
     1141 acaataccgg atttgacatg atataccagg aaaacaaggt gttcaactta aagctggcag
     1201 caaagacctt aaacggctta ttgataagac cgggggaaac cttttctttc tggtggctgg
     1261 tacgccatgc ggacaaagat accccctata aagacggcct tacggtgacc aatggtaaac
     1321 tcaccaccat gtcgggcggc ggtatgtgcc agatgagcaa tttactattt tggatgttcc
     1381 tgcatacgcc attgacaatt atccagcgca gaggtcacga agtaaaggag tttccagagc
     1441 caaacagtga cgagattaaa ggggtggatg caaccattcc agagggctgg attgatttaa
     1501 aagtgcgaaa cgataccgac tgcacctacc aaatatgggt gaccttagat gatgagaaaa
     1561 tcatcggtca ggtgtccgcc gacaaagagc cccaagcatt atacaaaatt acaaatggca
     1621 gtatccagta tgtccgtgaa agtggcggga tttatgaata tgcccaggtt aaacggatgc
     1681 aagttgcctt aggtaccggg gaaataatag attgcaagct gctttataca aacaaatgca
     1741 aaatctgtta tcccctcccg gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag
     1801 tgtgggcatt actgtttttg gatgcgaaca ggatgaggca aatgctttcc gcgctttatc
     1861 gccggatttt catattatcc ctacgctgat tagcgatgcg atatcggcag acaacgcaaa
     1921 attggccgct ggcaatcaat gcgttagcgt aggccataag tccgaggttt ccgaggcgac
     1981 aattcttgcg ctgagaaagg tcggggtaaa atacatttct acccgcagca tcggctgcga
     2041 tcacattgat acgactgccg ccgagagaat gggaatctcg gttggcacgg ttgcgtattc
     2101 gccggacagc gttgcggatt atgctttgat gctgatgctg atggccatac ggggtgcaaa
     2161 acccaccatg cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc gggggaaaga
     2221 actgggggat atgactgtgg gagttattgg aaccggccat atcgggcaag cggtcgtcaa
     2281 aaggctgcgg ggatttggat gccatgtgct ggcctatgat aacagccgaa aaatggatgc
     2341 agattatgtc cagcttgatg agcttctaaa aaacagcgat attgttacgc tccatgtgcc
     2401 gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa attggagaga tgaagcaagg
     2461 cgcattttta atcaacaccg cgcgcggagc gcttgtcgat accggggcgc tggtggaggc
     2521 gttggaaagc ggaaagctgg gtggtgcggc actggatgtg ttggagggcg aggatcagtt
     2581 tgtttatacc gattgctcgc agaaagtgct tgaccatccc tttttgtcgc agcttttaag
     2641 gatgccgaat gtgatcatca caccccatac ggcgtactac actgagcgtg tgctgcagga
     2701 tactacagaa aaaacaatca ggaattgtct caattttgaa aggagtttac agcatgaata
     2761 gaataaaagt cgcaatcatc ttcggcggtt gctcggagga acatgatgtg tcggtaaaat
     2821 ccgcaataga aattgctgcg aacattgata cggaaaaatt cgatccgcac tacatcggaa
     2881 ttacaaaaaa cggtgtatgg aagctatgca agaagccatg tacggaatgg gaagccgaca
     2941 gtctccccgc catactctcc ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa
     3001 gcgaatacga aacacggcgt attgatgtgg ctttcccggt tttgcatggc aaattcgggg
     3061 aggatggtgc gatacagggg ctgtttgtat tgtctggtat cccctatgtg ggctgtgata
     3121 ttcaaagctc cgcagcttgc atggacaaat cactggccta cattcttaca aaaaatgcgg
     3181 gcatcgccgt tcccgaattt caaattattg ataaaggtga caagccggag gcgggtgcgc
     3241 ttacctaccc tgtctttgtg aagccggcac ggtcaggttc gtcctttggc gtaaccaaag
     3301 taaacggtac ggaagaactt aacgctgcga tagaagcggc aggacaatat gatggaaaaa
     3361 tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg
     3421 atgatttgat tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc ttccgcatcc
     3481 atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat gattacagtt cccgcagaca
     3541 ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg
     3601 gatgcagagg gcttgcccgt gttgatcttt ttttgcagga ggatggcggc atcgttctaa
     3661 atgaggtcaa taccatgccc ggttttacat cgtacagccg ctacccacgt atggtggccg
     3721 ccgcaggaat cacgcttcct gcactgattg acagcctgat tacattggcg ttaaagaggt
     3781 gataagtatg gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg tccgttggga
     3841 tgccaagtac gccacatggg ataacttcac aggaaaaccg gtggatgggt atgaggtgaa
     3901 tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc
     3961 tgcgcttggc tatggcttgc ttttatggga tggatatcgg ccaagaactg cggtggactg
     4021 cttcctgcgt tgggcagcgc aaccggagga caatctcaca aaagaaaaat tttaccccaa
     4081 tatagagcga gccgagttga ttacaaaggg ttatgtggcc tcacaatcca gccatagccg
     4141 tggaagcgca attgatctta cgctctacca cctggataca ggggaacttg tttcaatggg
     4201 aagtaacttc gattttatgg acgaacggtc gcatcataca gcaaaaggga taggggatgc
     4261 agaggcacaa aatcgaagat gcttgcgtaa aatcatggaa agcagcggat ttcagtctta
     4321 tcgctttgaa tggtggcact ataagttgat tgatgagcca taccccgata cctattttaa
     4381 ttttgccgtt tcataatgaa agtattggtt ttgtatcaga ttttggattt gataaaatct
     4441 atgctggtca tggacaatct tttactggaa agtggttaaa gcatcttttg taatacattg
     4501 caaattatag tattcaatct gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac
     4561 ctttcccacg cccattttgt attttgctgg cggcggtttt gagaaatcaa ggccgccgtt
     4621 cttttctggc agcaaaaggt gtggcggccc ataggaggat gccgctatgc tgttttgcta
     4681 ttgccatgct ctaatccact tccaccactc aaaaaagcct tgcccctcca cagggctatt
     4741 ccggttatga gtatgtacta taccatgtaa atcagcagca tagcaaagca ttttctcgcc
     4801 cggtaggttt gcgacctgcc gggcgatttt tgtcgtcttt tgcggcccgg ctgtccccaa
     4861 aaaaattttt tgagaaattt tcaaaaagga ggtatttagc gggtagctgg cggccttgtc
     4921 aaagtcttgt tagcggaaag ggagagaacc acctaagtcc cccacagaaa gggggtgaga
     4981 agatggatgc tatcccccgc gactatgaag cgcggtgcat gttcgatgct ttttgcaaga
     5041 cggtactgcg taacgaggcc aaaagctact tggctgaaat gaagcgccgc cgtgaccgtg
     5101 aagtgtcact aagctctttg tcacaggcag atttggacaa gctctgtaca gtagatcact
     5161 accccagcga cagctttacg ttttcgtcgc atggctatga cctacatatc aacaatgagc
     5221 ttgttgccga agcctttgcc gcgctgccca gcatggagca gagtatttta atcctgcact
     5281 gtgttctgga tatggcagat ggtgagatcg gcggccttgt gggaatgtcc cggagtgccg
     5341 ttcagcggca caggacaaat accttatcgg agctgcggaa acagctaaag gcgttgatgc
     5401 cgaagggagg ttagacgcgg atgaagaacc cgaactacaa gggcattgag cttttgcccc
     5461 tttcggtgat cgaggccgcc cgcgctgggg atgccggagc cgtagagcgc gttttgcagt
     5521 attacagcgg ttacatgaac aagctgtgta cccggacgct ctatgacgaa aacggctatc
     5581 cgcatgtgtg tctggacgag gccatgaagc gccgtctgga aatcaagctc atccacgcta
     5641 tcgtcacgaa ctgacgaacc ggccccggca gcatgaaacg gcttaccctt tccccgtttc
     5701 ggtgttttcc cggtggcctg atcgcacctt gataaatatg cccgcaagat aacggcggcg
     5761 catcataggg catacggaca cattctgtct gtgccgagcc ggacggctgg tgcgccatga
     5821 tacttttccc tacgggaaaa gcggagcgag gaacaccacg ccgcaatgtg agcgtggcgg
     5881 cttgcgggcg acgacgtaca ggcaggacta tgatactccc ttatagccac agtccgagcg
     5941 ttaaaagcgt cgcaggcaat gggtagggcc gcgtgagaac cacgcggggg tgaaagtccc
     6001 gtggagctgt gccagcagcc gtccgtctat gcccctaaat cacacaaacc tttgaatttg
     6061 tgaaaggggg ctgcattatg aaccatgatg tgcctatttg ggaaaaatac acgctgacga
     6121 ttgaggaagc gtctaaatat ttccgtatcg gagaaaacaa attgcgtaag ctggccgagg
     6181 aaaatccggc ctctggctgg gtcattatga atggcaaccg catccagatc aaacgcaagc
     6241 agtttgagaa aataattgac actttggaca caatctaata gtgaaaaagg gccgcagata
     6301 tgttataata atttataagc atatatgcgg ctctttccgt catggaaagg agcatgacga
     6361 gatgtcagaa aaaagacgag acagcaaaaa tcgcgttttg cggtctggag agagccagag
     6421 aaaagacgga cgatatgctt acaaatatgt tgataccttt ggaaaaccgc agtttgttta
     6481 ctcatggaag ttagtgccta cggataagac cccggcaggt aaacgggagg atatttcact
     6541 ccgggaaaag gagaaagaaa tccagaaaga ccttgacgac gggatagaca ccatcggcaa
     6601 gaaaatgacc gtctgccagc tctacgccaa gcagatcagg caccggggca atgtgaagca
     6661 caacaccaaa ctgggccggg aacggctgat gcgtattctg gaacaagatc ggctggggag
     6721 ctgcccgatt gacagtgtga agctgtccga cgcaaaggaa tgggcgcttc ggatgaaaga
     6781 aaagggcctt tcttataaga ccatcaataa cgacaagcgt tccctgaaag cggcttttta
     6841 caccgccata caggacgatt gcatccgaaa aaacccgttt gacttccagc ttagtgacgt
     6901 tctggacgac gatacggaac caaaggtgcc tcttacacca gcacaggaag aaagttttct
     6961 gtcctttatt caaggtgata aggtttacca aaagcattat gacgcaatcg ttattctgct
     7021 ggggacaggg cttcgcattt cggaactgtg cggactgacg gacaaagatt tggattttga
     7081 aaaccgggta atcatcgtga gccaccagct tttgagaaat accggcgtcg gttactatat
     7141 tgacgaaccc aagacccaaa gcggcgtcag aaaaatcccc atgaatgagg aagtgtatca
     7201 ggcgttccag cgagtgataa aaaaccgcaa aggtgcaaag ccgttcatta ttgacgggta
     7261 tgccaatttc ctgtttctca aacagaacgg ctatcccatg acggcggtag attatggcgg
     7321 gatgtttggc aggcttgtga agaagtacaa caagagccat gaagaagcgt tgccgaaaac
     7381 gacgacgccc catgctatgc gtcatacatt ctgcacccgg ttagccaatg cgggaatgaa
     7441 ccccaaagcc ttgcagtaca ttatgggaca ttccaatatc accatgacgc tgaactatta
     7501 tgcacacgcc acattcgatt ccgcaagggc agaaatggaa cggctggcag cctgacacaa
     7561 ggccggtttt actactccat ttactacttt tgagggggaa atggtgagag gtaataagaa
     7621 catttgtaag tatctaacaa tatcaaaaat gccggaaaag cccggaaaca cgggctatac
     7681 cgacattcaa gaacttctaa aaagataatc taaaatccca tataattttt cctcaaaagc
     7741 tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta tcggtagtcc
     7801 catctttcag attccatata ttggactagt cctttcaaaa acggcatttg catggattca
     7861 ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga atacctttgt
     7921 agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt aaaaacataa
     7981 atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac actcatgcag
     8041 cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg
     8101 aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt acccagcaaa
     8161 aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca gaagtaccag
     8221 ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt cagcctgaac
     8281 gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc acgacaatcg
     8341 gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag aaagctaaaa
     8401 aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt caagcagcag
     8461 atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt gcaaaatcaa
     8521 aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc gttttgatgg
     8581 tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt ccccgtggca
     8641 aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt
     8701 tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa
     8761 caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa tcgagggctc
     8821 aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat acaggaaatc
     8881 taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga taaccttaga
     8941 acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc caaaataatt
     9001 cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat tatctataat
     9061 tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat ccgccttcta
     9121 agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta gaaactgagg
     9181 tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa
     9241 ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa ctcatcagaa
     9301 aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt gcaattgaat
     9361 ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca agtagggttt
     9421 ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt aagaaccaag
     9481 gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt tcttccgcat
     9541 ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta tatggcagtt
     9601 tgactagcga tcgttggatt ttactcaaac aaggtgggct agctctgttg gcattgcttt
     9661 ctattttctt ataaatatag aaaaaataag aatttatcta acaggaaact ttgtgaaaaa
     9721 tattacttta tctcatgaaa tatgatattc taatgaatgg tattttagaa aatttcatga
     9781 aaaaggagtt agataggtgt tcaaaaatta tgtttcctca ctaaaaaaag aattttcagg
     9841 ttatgatctt tcaagatttc agaaagatct tttggctggg atcacagtag ctgctgtagc
     9901 attgccttta gccttggctt ttggggtatc aagcggagct gatgcggccg caggtttgat
     9961 tacggctatc attgccggta tcgttattgg cggtttatca ggcggttttt atcaaatatc
    10021 aggtccgaca ggtgcgatgg cagctatctt gatgtctatt gcggcaacaa agggaatgca
    10081 aggtgtattg cttgctactt ttttagcggg tctattttta cttgcagcag gacttttccg
    10141 attaggaaca ctgacttctt ttatcccagc acccgtaatt acggggttta cttcaggtat
    10201 tgctatcatt attgcattag gtcagatcga taatttattt ggtgttcact cggaaggtac
    10261 aaacgttatt gaaaaactaa gtagttatca aacgatagga tttccgataa acacagcctc
    10321 tttgatcatg ggagggctag tcattttagg gatgtttatc tatccaaaaa aatgggcgaa
    10381 acgaatgcct agttcgctgc tggctatcat tttggcaaca gcagttatgc tattgttcca
    10441 tttaccagta gcaactgtgg gtaagattcc acaaacattg atcagtagta atcgtttgaa
    10501 tatgggagat tttagctttt ctgccttgca acaagtggca gttccagcta ttagcattgc
    10561 tttattagga atgattgaaa gcttgctatg tggtgcttcg gctggccgaa tggcaaataa
    10621 gcccttagat agcaatcaag aattaatggc gcaaggaatt ggaaatctgt tgcttccttt
    10681 ctttggcggg attcctgcta ctgcggccat cgcccgtaca agcgtggcca tcaaatctgg
    10741 tgcacaaact agattagctg gtatgataca tgcagttgtc ttactgctct ccatgcttgt
    10801 ttttgcgcca gtgatgtcac agattccaat gccagcactt gcaggtgttt tgattgtgac
    10861 ggcttggcgt atgaatgaat gggaaacgat caaggagcta tttactaata aatattggtc
    10921 agcagttcta ttgtttatcc ttacgatgat ttgtacagtt atctttgatt taagtattgc
    10981 tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt gtcaaaagcg ctgctatcac
    11041 gatttcatta gaaacgatcg attggcaaag aatggggctg ccagaaacgg aaaagctgaa
    11101 taactggaca gtagtgtata ttagtggtcc actgtttttt atgtcttcag aaaaactcaa
    11161 aaataaattg gaagaactaa atcatcacga tggtgtcatt ttctccatgc gaggcgtacc
    11221 aagtattgat ttgacggcac gtgcagtttt tgatgaattt caagaaaaag cagagcaaag
    11281 aggacaaacg attatctata cttccttgca gccagaagta gaaaaacaac tttctcactt
    11341 gtgggaacat acattcacgc atcaacatca aacagttgct catgcgttga cagctttaca
    11401 tgcgaaatat actgcaatta gccattaaaa caatgaaaaa gaacagacaa gactgtaaac
    11461 tgaagatttt atggtcttgt ctgttctttt taatattgct gtaaaagtga agctagtact
    11521 tgccagtctt gatggataac aggtacaagg gagcgattgt agaaaatagc tgctggatga
    11581 aaaagtggaa gaattcgata gtttttttct gaccattcgt aggcatcctt ttgttctgtt
    11641 aattgtaaga caggccctgt atagagttga ccatgatttt tggtaaccgt atattctttg
    11701 ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa ttaattttgg ctggaccttc
    11761 atcagttcgt aatccaatat tggtgcatgc gcaaatactt cggaacgagt aggtgtccga
    11821 tttggataaa cagtttctga ctgttgtgtc cgtttattga tccgatgagt cacgcgatac
    11881 gggcggctcc ttacagcact ggtgatatac acctcttctc gtgttaagcc agttgaagaa
    11941 agtgcctgca tcaattcttt tccagcttga ccgctaaaag gaacatgcgt atcaatctct
    12001 gttttccctg gggcttctcc cacgagcatc agtttaggat gaagaggtcc ttgcccagcg
    12061 ataaatcctt ctacaggacg gccgttcatt cttaaagaag cttcttgtat cagttctttt
    12121 gggtattcca tttatgctcc tcctttttct tttattataa gaataaaaga aacacaatgc
    12181 gactatttta tttggtagaa ctgtgaggat agaaaagcag aagtattgct tgaatttttg
    12241 ttttactttt tgattttaaa tttagttgac taacggttgc tgttctggta agctagtact
    12301 gttgtaattg tggactccac agctacaacc gctcagaaca agtaataagc acagcatatg
    12361 ctgtcacttc ggatggcgag tctaagtata tcagaaggag gtgctttata gcatgtacgc
    12421 aattattaaa actggtggta aacaagtaaa agttgaagtg ggacaagcaa tctatgttga
    12481 aaaacttgac gttgaagcag gagaaaaagt tgtttttgac gaagttatct tagtaggcgg
    12541 agaatctaca aaagtaggcg ctccaactgt tgcaggtgca actgttgaag gtactgttga
    12601 aaaacacggc aaacaaaaga aagtcgttac tttcaaatac aaacctaaaa aacattcaca
    12661 ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt atcgacgcga tcaacgctta
    12721 atagcgtttg agaaagaagg cctatatatg atcaaaggaa catttaagcg aaatgacgca
    12781 ggtcagatcg tttcattcac tttgaccggg catgctgatg ctggtcctta tggaagtgat
    12841 atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg tcaacgggat tgcctcatta
    12901 gccggttttg agccgattgt tgaaatgaat gaagaagaag gcggttatct ttataccgaa
    12961 gtcacttcag gaatgacaca agaacaaaac aatattgctg agattctatt agaaaatctt
    13021 ttattaggat tacaatcgat agaagctgaa aactcagaat atatccaaat taaaacaatt
    13081 aatgataaat aggaggtgca gaccatgttg ttatctatga atctacaatt atttgcccac
    13141 aaaaaaggtg gcggttctac atctaacggc cgtgactcag aatcaaaacg tctaggtgct
    13201 aaacgtgctg atggacaaac agttacaggt ggatcaattc tttaccgtca acgcggtaca
    13261 aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg acactttatt tgcaaaagtt
    13321 gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa aacaagtgtc tgtttatcca
    13381 gtagctcaag aagcttaata aataaactgc tctatctctt gtgtagcaag ggatggagct
    13441 tttttgtaaa ttatgggatt ttttttgacc atacttttga ccatactttc ttagaagtta
    13501 atataatttg ttagtttttg tgttgtttct tcttttttgc tgttagtaat gtgtgtatat
    13561 gtgtccatag tagtttttat atttgcgtgg cccattcttt cttggacttc tttgtgatct
    13621 gcgcctgcct catatagcat tgatgcatgt gtatgtctaa aaccatgcag accgatataa
    13681 ggtaagccgg cacgctcacg atatatattg tatctttctg ccactgactg attaattgtg
    13741 aaattatttt cgtcattggt aaacaaataa ttagctttag taaatcctaa ttgaaataag
    13801 tactttcttt gttcgagctt ccaaaatttt agttgcttta tagttttttc atctaaataa
    13861 attattcgat ttgatttttt ggttttagga gtttcagata tataatattt atctgaacga
    13921 gctacagttt ttttaatggc catttcattt gttttaaaat taatattgtc ccaagtgagt
    13981 gctaatattt cgccgattct acatccgctg aatgctaata gtctaaagag cgtatagtct
    14041 ctgtttttaa aatatggatt ctgttcttga gatacttcat ttagaaataa ttccaactgt
    14101 tcttttgtat ataattttaa tttcttttct gttttaattt gtggctttgg aataagtaaa
    14161 tttaatgttg gattatcaga tgttaaacca atatttatag catacttaaa aactttattc
    14221 atatagttta tgaaaagagg gtactgcttt gaagttcctt tttcactcca atcatttact
    14281 accttttgac aatatgccgt attaatttta gaaagcttaa tatttccaaa tttgggatat
    14341 atatgtttat taaagattat tttagtcctt gaatatgaac tttcttttac agtttttttg
    14401 tagctttcta gccataattc tgctgcttct ttgaaagtag tatttgtact tgtgggcatt
    14461 cctgtttttt gtaattctag ttctaaccta gaaagtgctg tttttgcttc tttttgtgtt
    14521 ctaaatcctc gtttagtagt atattttttc tttccagtta gcggatcaat accgagataa
    14581 gttttaaagt accatgcttt ttcgccattt ttctttttat attgttttat cattgccatc
    14641 aatattaccc ctattcaaat tctaattctg ccatgctatc attgaaaaat tcgatcagtc
    14701 cttgttcctc tataaattca agccatattt ccttcccgtt aattaatgga actccttttt
    14761 ctttacgaat tgtttgatac aagttagtca cgacttgttt ccacatttct aatacagctt
    14821 tatctatttt cttatcatcc cagcgttttt tttggggtct atttttgttt ttttcaattt
    14881 gataattagt agctttgatg ataaatgttt cgtatccggt tgtgcttttt tctaaccatt
    14941 ctttgaattc tgtatatatc atttttgttc acctttctat ttttgaacgt atgttctttt
    15001 ttgcttaaaa aattttagag agctgcataa caatagatgc gagcttttaa taattctttc
    15061 acaagtggtt cgaagcgaac gcttaaattg ttatcttcta taaatttcat gtaattgata
    15121 ttgtgtggtt ctaattcaga ctttgcaata tatctatcta ataatttttc aatcatgaag
    15181 cgatcagcct catattccat ttttgaatgg agagagaatg ctaagttata taaaaaataa
    15241 ttatcatgat gtttagaagc gtgtcctaat tcatgtaata aggttctttg tcttctatac
    15301 ttagataaat tagcttttat tactatggta ttcatacaag caacataatg gccatcggca
    15361 tcaaggtttt cacgctcttc taccttaacg ccgagttcat taattatcat ttcaatttga
    15421 ctgtccaaat gactcaccta cttatttagt tatctttttt ctctaaatat gcttcaatca
    15481 tagctgatag tatttcccta tcattatcgg tgagcggttt tccgtcgcta ctcataacag
    15541 atgctaaagc ttcttccacg gttagttttc tttcctctgg tgcaactccc gcgttaggat
    15601 catctgtgcg tcctagcaaa taatctgttg atacatggaa gtagtcagca actttttgca
    15661 aagtttctat tttaggactt tgtgtcttcc atttataaat agagtttttt ccaaatccta
    15721 ttctattttc taattcagaa atagaaatac ctcttttttt acagagctct ttaacccttt
    15781 cgaaaagcat gtcaaatcaa ccttcctaga gcgtacgata ataatttata caaaaagata
    15841 gaaaggtagt tgacaaatct atccaattgt cttatattta actcgtaagc taatttaata
    15901 agctaacaag caacaagaaa tacacctaat aaaataaacg aattcgaggt cggcaaacta
    15961 agaatgttaa tttttagggt ttttctaggc ttatttaact gtatataaat ataagacagt
    16021 tggatagaaa tgtcaataaa aattagctaa ttttattagc ttacatattt aaagaaaagg
    16081 aggaaacatc atgaaacaaa ataaaaaaac tccttccgca aaaggagctt taataacttt
    16141 aaattttaaa gaccaaaagt ttagtgctgt tgttgatgaa attcatccac aagagcatca
    16201 aaaaaattct tttgcaactg acgagaaaaa gactcagaaa cgctctgaga aagttcttcg
    16261 aataattcaa tttctgttgg aaaagattcg tcattttgga agtctatttt gaattcttta
    16321 ttaacagtct tgaatacggt ttgtttgaat aaggcgtcga catccatatt aaattcttcc
    16381 atactatttt cacctcgctt tctattcaaa attatatcaa agaaaggaat gagcaaaaat
    16441 gaacacaccg caaattttta actttgaaca acatgaagta agaacagtaa caatacatga
    16501 tgaaccattt tttgttggaa aagatgtggc taaagttcta gggtaccaaa atggtagtag
    16561 agatattaac agacacgtcg atgtagaaga tcggcaaaac taccaaaacg gtacttttga
    16621 atcgcctaga gggttaacta ttattaacga atcaggtttg tactccttaa ttttaggaag
    16681 caaacaacca aatgctaaga aatttaaacg ttgggtgact agcgaagtgt tgccagcaat
    16741 tagaaaacat ggaggttatc taa
//
LOCUS       DAGCXG010000293        16663 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.049, whole genome shotgun sequence.
ACCESSION   DAGCXG010000293 DAGCXG010000000
VERSION     DAGCXG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.049"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            complement(134..577)
                     /locus_tag="MB022_002976"
     CDS             complement(134..577)
                     /locus_tag="MB022_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4467884.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="MB022_002977"
     CDS             complement(795..2135)
                     /locus_tag="MB022_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4467885.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="MB022_002978"
     CDS             2404..3300
                     /locus_tag="MB022_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4467886.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="MB022_002979"
     CDS             complement(3450..4391)
                     /locus_tag="MB022_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4467887.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="MB022_002980"
     CDS             4517..5638
                     /locus_tag="MB022_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4467888.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="MB022_002981"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="MB022_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4467889.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="MB022_002982"
     CDS             7426..7992
                     /locus_tag="MB022_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4467890.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="MB022_002983"
     CDS             8203..9264
                     /locus_tag="MB022_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4467891.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="MB022_002984"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="MB022_002984"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBT4467892.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="MB022_002985"
     CDS             10177..12159
                     /locus_tag="MB022_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4467893.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="MB022_002986"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="MB022_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBT4467894.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="MB022_002987"
     CDS             12881..14305
                     /locus_tag="MB022_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4467895.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="MB022_002988"
     CDS             complement(14486..15259)
                     /locus_tag="MB022_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4467896.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="MB022_002989"
     CDS             complement(15256..16161)
                     /locus_tag="MB022_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4467897.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="MB022_002990"
     CDS             complement(16315..16635)
                     /locus_tag="MB022_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBT4467898.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5367 a         3044 c         3435 g         4817 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaa
//
LOCUS       DAGCXG010000294         8053 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.107, whole genome shotgun sequence.
ACCESSION   DAGCXG010000294 DAGCXG010000000
VERSION     DAGCXG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.107"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            62..979
                     /locus_tag="MB022_002991"
     CDS             62..979
                     /locus_tag="MB022_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4467899.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            982..3159
                     /locus_tag="MB022_002992"
     CDS             982..3159
                     /locus_tag="MB022_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4467900.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3156..4157
                     /locus_tag="MB022_002993"
     CDS             3156..4157
                     /locus_tag="MB022_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4467901.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4154..5086
                     /locus_tag="MB022_002994"
     CDS             4154..5086
                     /locus_tag="MB022_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4467902.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5331..5447
                     /locus_tag="MB022_002995"
     CDS             5331..5447
                     /locus_tag="MB022_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBT4467903.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5463..6278
                     /locus_tag="MB022_002996"
     CDS             5463..6278
                     /locus_tag="MB022_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4467904.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKKTTSCIYTPL"
     gene            6343..7383
                     /locus_tag="MB022_002997"
     CDS             6343..7383
                     /locus_tag="MB022_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBT4467905.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            7502..7669
                     /locus_tag="MB022_002998"
     CDS             7502..7669
                     /locus_tag="MB022_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBT4467906.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7729..>8053)
                     /locus_tag="MB022_002999"
     CDS             complement(7729..>8053)
                     /locus_tag="MB022_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4467907.1"
                     /translation="RAFGLAIKEARLKRGLTREQVGALIEIDPRYLTNIENKGQHPSI
                     QVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIMESLASGINEA
                     RNIED"
BASE COUNT         2615 a         1455 c         1662 g         2321 t
ORIGIN      
        1 aaacttggag ataacatcaa aaagttacct atcgctataa cgtggcagat ggaaagaaac
       61 cttgccagag atttcccatg aaatcaatat cgtcactctg acttctgatg agaaaaacaa
      121 aggcttactt gacccttatg tgattatgaa aaatcccaaa gattctgaat cactggctat
      181 tgatattctg acattcctta cggggatttc ctctcgtgat ggggaacgct tcccaatcct
      241 tagaaaagcc attcgtgcag taaccaatag tgaagtacga gggttgatga aagtgattga
      301 ggaattacgg gttgagaata cgccactaag taccagtata gccgaccata tcgaaagttt
      361 tacagactat gactttgcac atttattatt cagtaatggt tatgtggagc agtctatcag
      421 cttagaaaaa caactgaaca ttatacaggt tgcggacttg gtacttcccg acaaggaaac
      481 ttcctttgag gaatatacca ctatggagct tttatccgtt gctatgctga ttgtcattag
      541 tacctttgct ttagacttta tccatacaga ccgaagcatt ttcaagattg tagatttaga
      601 cgaagcatgg agctttttac aggtagcaca aggaaaaaca ctatctatga agctggttcg
      661 ggctggtcgt gctatgaacg ctggggtata tttcgtgacc caaaatacag acgacctctt
      721 agatgaaaaa ctgaaaaata acctcggctt aaaatttgca tttcgttcca ctgaccttaa
      781 cgagattaaa aagaccttag ccttttttgg tgtagaccca gaggacgaaa acaatcagaa
      841 gcgattgcgt gatttggaaa acgggcaatg ccttatcagt gatttatatg gtcgtgtcgg
      901 tgtgatacag ttccaccctg tatttgaaga actgctccat gcctttgata ccagaccacc
      961 tgtgcgaaaa gaggtgtaaa tgtgaaacca tcaatagtaa acagaataaa atcaaactgg
     1021 acgctgaaac gtctaggtaa agtggcaatg acagtggctt tcacacttgt gattgccatt
     1081 tttcttttag ccatgctggg aacggtggtt caagctgcgg gcttggtaga tgatacggtc
     1141 aatgtggcaa atgaatacag ccgataccca cttgaaaact atcaactgga tttttatgtg
     1201 gataatagct ggggctggct tccgtggaac tggtcggacg ggattggaaa acaggtcatg
     1261 tatggactat atgccattac caattttatt tggacaatca gtttgtatgt ttccaatgcg
     1321 acaggttact tagtacagga agcctattcc ttagacttca tttccgctac agcagattcc
     1381 attggtaaga atatgcagac cttagctggt gtgagtgcaa acggattttc aacagagggt
     1441 ttctatgttg gattcctctt actcttgatt ttggttcttg gggtttatgt tgcctatacg
     1501 ggactgataa agagagaaac cacaaaggca attcatgcca ttatgaattt tgtgctggtg
     1561 tttatcctat cggcttcctt tattgcctac gctcccgact acattaaaaa aatcaatgac
     1621 ttttcatcag acatcagtaa tgccagttta tcacttggca cgaagattgt catgccccat
     1681 tccgatagtc aaggcaagga cagcgtggac ttaatcagag atagcctgtt ttccatacag
     1741 gttcagcaac cgtggctact gcttcaatac aacagttcag acattgaaag tatcggtatt
     1801 gaccgtgtgg aaagcctgct ctccaccagc ccagattcca acaatggcga agacagagaa
     1861 aaaattgttg cggaagaaat tgaagacaga agcaatacca atctaaccat tacaaagacc
     1921 attaaccgtt taggtacagt cttcttccta tttgtcttca atattgggat ttccatattt
     1981 gtattcctat taacaggaat catgattttc tcgcaggtac tttttatcat ctatgctatg
     2041 tttctgcctg tgagctttat tttaagcatg attccatcat ttgatggtat gtcaaaacga
     2101 gccataacaa agctctttaa taccattttg acacgagctg gaatcacatt gattattacg
     2161 acagcattta gtatttcaac catgctctat accttatcgg ctggttatcc gttctttttg
     2221 attgcttttc tacagattgt gacctttgca ggaatctact tcaagctggg cgatttaatg
     2281 agtatgtttt ctctacagag taacgattct caaagtgtgg gaagtcgtgt gatgagaaaa
     2341 cctcgtatgc ttatgcacgc tcacatgcac cgtctacagc ggaaacttgg acgttccatg
     2401 actactctag gggctgggtc tgccattgtt acaggtaaaa aaggacagtc gggttcgggg
     2461 agttctgcaa ggacacaagc agatcactcc cgaccagacg gaaaggaaaa atcaacactt
     2521 ggaaaacgta tcggtcaaac catcggtaca gtagctgata ccaaagacag aatggtagac
     2581 actgctagtg gtttgaaaga acaggttaaa gatttgccga ccaatgcaag atatgcagta
     2641 tatcaaggaa aatccaaagt aaaagagaat gtccgtgatt taaccagtag tatttctcaa
     2701 accaaagcgg acagagccag tggacgcaag gaacagcagg aacaaaggcg aaaaaccatt
     2761 gcgaagcgtc gctctgaaat ggaacaggtc aaacagaaaa aacagcctgc ttcttctgtt
     2821 catgaaagac cgactacaag acaagaacaa tatcatgatg aacagacctc aaaacagtct
     2881 aatattcaga cttcatataa ggaatctcaa caagccaaac aagagcgtcc agcagttaag
     2941 tccgattttt caagtccaaa agtggaacgc caaggcaata ccgttcaaga aaaaaccgtt
     3001 caaaagccag caacttcaac cactacagca gatagaactt cacaacgtcc aatcacaaaa
     3061 gaacgtccgt ctactgttca aagagtacca ctacaaaata caagaagtag accaccaatc
     3121 aaaaccgcca ccattaagaa agtcggtaag aaaccatgaa gttgaaaact ttagtgattg
     3181 gtggttctgg attattcttg atggtcttct cactgcttct gtttgttgcc attttatttt
     3241 cagatgaaca ggacagcgga atttccaata ttcattatgg aggtgtgaat gtttccgcag
     3301 aagtgctggc tcataagcct atggtagaaa aatatgccaa agaatatggc gttgaagaat
     3361 atgtcaacat acttcttgcg attatacagg tggaatcggg cggtactgcg gaagatgtta
     3421 tgcagtcctc ggaatccctc ggtcttccac ctaattcatt gagtacagaa gaatccatta
     3481 agcaaggtgt gaagtatttc agtgaattat tagccagtag cgaaaggctc agtgtagatt
     3541 tagaatcggt tatccagtcc tacaattatg gtggtggttt cttagggtat gtggctaatc
     3601 gtggaaataa atataccttt gaactggctc aaagtttctc aaaagagtat tcaggtggcg
     3661 aaaaagtgtc ttaccccaat cccatagcca tacctatcaa tgggggctgg cgatacaact
     3721 atggcaatat gttttatgtg caactggtaa cgcagtatct tgtcacaaca gagtttgatg
     3781 atgatacggt acaagccatc atggacgaag cactgaaata tgagggctgg cgatacgttt
     3841 acggtggagc ttccccgact acttcttttg attgtagcgg actgacacaa tggacgtatg
     3901 gaaaagctgg aattaactta ccacgaaccg cacaacagca atatgatgtg acccagcata
     3961 tcccactatc ggaagcacaa gctggcgatt tggttttctt tcattctacc tataacgctg
     4021 gctcttatat tactcatgtt gggatatacc ttggcaataa ccgtatgttt catgcaggcg
     4081 acccaatcgg ttatgccgac ttaacaagcc cctactggca acagcattta gtgggagcag
     4141 gacgaatcaa acaatgagaa aggaagattt aatgatgaaa tttagaaaaa atcagaataa
     4201 agaaaaacag ataccaaagg aaaagaaacc tcgtgtctat aaggtcaatc ctcataaaaa
     4261 ggttgtgatt gccttgtggg tacttttagg gcttagtttc agctttgcga tattcaagca
     4321 ctttacagct atagatactc atactattca cgaaacaact atcatagaaa aggaatacgt
     4381 tgatactcat catgtagaaa attttgtaga gaactttgcg aaagtctact attcatggga
     4441 gcaatccgat aagtccattg ataatcgaat ggaaagtcta aaaggctatc tgacagatga
     4501 acttcaagct ctcaatgttg atacagtacg caaagatatt cctgtatcgt cttctgtaag
     4561 aggatttcag atatggacgg tagagccaac tggcgacaat gagtttaatg taacctacag
     4621 tgtagaccag ctcattacag agggagaaaa tacaaagacc gtccactctg cttatatagt
     4681 gagtgtctat gtagatggtt ctggaaatat ggtactggtt aagaatccga ccattaccaa
     4741 catacctaag aaatcaagtt ataaaccaaa agccattgaa agtgagggga cggttgattc
     4801 cattacaacc aatgaaatca atgagttttt aacgacgttc ttcaagctct atcctacagc
     4861 gacagccagt gaactttcct actatgtgaa tgacgggata ttaaaaccaa tcggaaaaga
     4921 gtacatcttt caagaactgg taaatcctat tcacaatcgt aaggataatc aagtcacggt
     4981 atcgctgaca gtggagtata tcgaccagca gaccaaagca acgcaggtat ctcaatttga
     5041 tttggtactt gaaaagaacg ggagtaattg gaagattata gaataacaaa tattggtaca
     5101 ttattacagc tattttgtaa tcacgtactc tctttgataa aaaattggag attcctttac
     5161 aaatatgctc ttacgtgcta ttatttaagt atctatttaa aaggagttaa taaatatgcg
     5221 gcaaggtatt attaaataaa ctgtcaattt gatagcggga acaaataatt ggatgtcctt
     5281 ttttaggagg gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa
     5341 agtatccgga gaatatctgt atgctttgta tgcctatggt tatgcataaa aatcccagtg
     5401 ataagagtat ttatcactgg gatttttatg cccttttggg cttttgaatg gaggaaaatc
     5461 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa
     5521 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
     5581 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
     5641 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
     5701 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
     5761 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
     5821 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
     5881 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
     5941 tgtatcctaa tgtgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
     6001 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
     6061 tcgaacaaga ggaaagcata agatttcaga attgttctct gttccctctt tatcatggaa
     6121 gtgcaaaaag taatataggg attgataacc ttatagaagt tattactaat aaattttatt
     6181 catcaacaca tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa
     6241 aaaaaaagac aacgtcttgc atatatacgc ctttatagtg gagtactaca tttacgagat
     6301 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
     6361 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
     6421 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
     6481 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
     6541 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
     6601 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
     6661 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
     6721 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
     6781 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
     6841 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
     6901 gggatacgct atggttgtga acaaggattg tatggttgga atgtgacgga ctgtaaaatc
     6961 tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt
     7021 gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat
     7081 cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct
     7141 aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga
     7201 gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga
     7261 cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc
     7321 cagccccgtc gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact
     7381 tagtgtattt tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt
     7441 taataaagat ttgaattaaa gtgtaaagga ggagatagtt attataaact acaagtggat
     7501 attgtgtcct gtatgtggaa ataaaacacg attaaagata agggaagata ctgaattaaa
     7561 aaaattcccc ctctattgtc cgaaatgcag acaagaaaat ttaattgaaa taaagcagtt
     7621 caaagtaact gtgattacag agccagacgc aaagacgcag agccgataaa atgagattaa
     7681 tacaatctca ttttatcggc tctttccgtt atgtatggat tcttttaatt agtcttcgat
     7741 gtttcttgct tcgttgatac cgctggctaa agattccatt atggatagtt ctttgtctgt
     7801 aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg ctttttacca agttattagc
     7861 aggtaagaaa aattcatcaa cggaaacatg aagtaacgat acaaggtcat aaagaacttg
     7921 tatgctgggg tgttgccctt tattttcaat attagttaag taccgtgggt caatttcaat
     7981 caatgctccc acttgttcac gagttaaacc tcgtttcaat cgagcttctt taatggctaa
     8041 accaaaggct cta
//
LOCUS       DAGCXG010000295         7959 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.108, whole genome shotgun sequence.
ACCESSION   DAGCXG010000295 DAGCXG010000000
VERSION     DAGCXG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.108"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            128..1564
                     /locus_tag="MB022_003000"
     CDS             128..1564
                     /locus_tag="MB022_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4467908.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="MB022_003001"
     CDS             1753..4200
                     /locus_tag="MB022_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4467909.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="MB022_003002"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="MB022_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBT4467910.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="MB022_003003"
     CDS             complement(5690..6793)
                     /locus_tag="MB022_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4467911.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="MB022_003004"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="MB022_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4467912.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAGCXG010000296         5100 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.132, whole genome shotgun sequence.
ACCESSION   DAGCXG010000296 DAGCXG010000000
VERSION     DAGCXG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.132"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..83
                     /locus_tag="MB022_003005"
     CDS             <1..83
                     /locus_tag="MB022_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4467913.1"
                     /translation="LLDKLNTQLCNITTETKEVNNNGTKK"
     gene            67..696
                     /locus_tag="MB022_003006"
     CDS             67..696
                     /locus_tag="MB022_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4467914.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1050..2528
                     /gene="msr(C)"
                     /locus_tag="MB022_003007"
     CDS             1050..2528
                     /gene="msr(C)"
                     /locus_tag="MB022_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4467915.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2575..3021
                     /locus_tag="MB022_003008"
     CDS             2575..3021
                     /locus_tag="MB022_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4467916.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3220..3735)
                     /locus_tag="MB022_003009"
     CDS             complement(3220..3735)
                     /locus_tag="MB022_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4467917.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3852..5054
                     /locus_tag="MB022_003010"
     CDS             3852..5054
                     /locus_tag="MB022_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4467918.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1569 a          853 c         1100 g         1578 t
ORIGIN      
        1 cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg aaagaggtta
       61 ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag atccgttcgc
      121 gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat caaagaagcg
      181 acgactgcac cttcttctgt caatatgaaa ccatggcgct ttgtggtcgc tgaaagcgag
      241 gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa tgatacttct
      301 tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga agaaatctac
      361 aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca attagctgcg
      421 atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt caaagtcgat
      481 tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta tgaaacaaat
      541 ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga taaagaccgt
      601 tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta cgagtctgtt
      661 cgtttagcac cagaaaagat cactatgttc gaataaaagg ttttcagtta ctattcagta
      721 gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga
      781 ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca
      841 tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac agtagaatag
      901 gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga gtaggaaaag
      961 atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc gataattgtt
     1021 gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat ataacaaatc
     1081 tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt gtctatcagc
     1141 aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa
     1201 tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc ttcaactacc
     1261 tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta gccagtcatt
     1321 tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa gaacgaaaga
     1381 tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac gagccaacaa
     1441 cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat tattatggta
     1501 cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg aaaatctggg
     1561 aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat ctcactcaaa
     1621 aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa gagaaaaaac
     1681 gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca
     1741 gtaaaaagaa aaagcaacaa atcagactgg atcggttgtc ttcctctaaa caaaaagaca
     1801 gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag
     1861 gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg
     1921 aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa
     1981 cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg
     2041 aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc
     2101 ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg
     2161 aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta
     2221 gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa
     2281 gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt
     2341 tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc
     2401 taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc
     2461 gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa
     2521 atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct
     2581 acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg
     2641 atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt
     2701 ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg
     2761 aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat gatcttaact
     2821 atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg tgtgatcatg
     2881 gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa aatgaatgtg
     2941 attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa
     3001 gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt ggcatgcttt
     3061 ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat aggttttggc
     3121 ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga agataatttg
     3181 ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa actgctgcat
     3241 ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa ttctgcgctg
     3301 gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat tcaatttttt
     3361 ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca gtccagtagc
     3421 tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat ctttttgcgt
     3481 aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt catcagtcaa
     3541 gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat aaccctgctg
     3601 gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa aaattgtgat
     3661 cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata ataatatata
     3721 ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat atcacagtaa
     3781 agtttccacg ttttacatgt gcatattgac atgtaaaata aatcatgtat gatgctaata
     3841 gagagaaagg gatggaaata ttggagaatc aaatagatat acaagaacaa acaaatgaag
     3901 aaacaaaggg gaaaagttca gcgctagttg tagcagctac ctgtatgatc gcttttatgg
     3961 gagtgggtct agtagaccca atcttaaaaa cgattgctgc acagttaaac gctacgccag
     4021 ctgaaacgac cctgttattt actagttaca tgttagtcac tggtgtggtg atgctattta
     4081 caggtttctt atctagtcgg atcggtttga aaaaaacatt gatgacagga ttgtttatca
     4141 tcgttgtatt cgctggtctt ggcggattat ctgggaatat cggaatgctg attgggttac
     4201 gtgccggctg gggtgtcggg aatgctttat ttgtctctac tgctttagca acgatcgtca
     4261 gtattttagt tggtaacaca gaaaaagcaa tcatgatgta tgaagcagca cttggctgcg
     4321 gaatggctgt tggccctcta gtcggtgggg tcttgggcag tggttcatgg cgttatccat
     4381 tttttggggt agcatttttg atgttttgcg gttttatagc cctagcggta cttttacctg
     4441 aaactgagaa accaaagaga aaagtaggcc tttgggaagg tgtcaaagcg ttaagcaatc
     4501 ataaacttcg ttcagttgga ctaatcgcat tgttatataa tttcggattc tttactttac
     4561 ttgcgtatgc gccattttta ttgattggct attctgaatt agaagtcggt tttgtatttt
     4621 ttggctgggg tgtgcttctg gctattgctt cgattttcgt tgcaccggct cttgagcgga
     4681 agtttacgac ttatcggaca attttagcag catttgtctt attcattatt tgtttgctgt
     4741 tattaggagc tggaacttct atgccagtcc ttgtaccgat cagcattgtg ttagcaggat
     4801 ttttccaagg aattatcaat acactgttga cgacgattgc tatggaaata ccaggtctgc
     4861 aacgaaacgt tgcttcgtcc agctatagtt ttgtccgctt tttcggtgga gctttagcac
     4921 catttattgc aggtgtgatt ggtgaacata ttggtgaaag ctatgcgttt tattttgcag
     4981 gtgccattgt tcttatcagt gtatgttgta tcatttatca ccgtaattat tttgctacaa
     5041 atgaagaaga ataagaaatc agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa
//
LOCUS       DAGCXG010000297         2436 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.176, whole genome shotgun sequence.
ACCESSION   DAGCXG010000297 DAGCXG010000000
VERSION     DAGCXG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.176"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            53..1267
                     /locus_tag="MB022_003011"
     CDS             53..1267
                     /locus_tag="MB022_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBT4467919.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1298..1516
                     /locus_tag="MB022_003012"
                     /pseudo
     CDS             1298..1516
                     /locus_tag="MB022_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1935..2408
                     /gene="dfrF"
                     /locus_tag="MB022_003013"
     CDS             1935..2408
                     /gene="dfrF"
                     /locus_tag="MB022_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBT4467920.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
BASE COUNT          740 a          380 c          554 g          762 t
ORIGIN      
        1 aaacaactac accttctttt atactcgtaa tcgtagaaag gaggcgaaaa aaatgaatac
       61 attacctatc aatacaggag atacagcatt catgatttta tgtacagcaa tggtttgcct
      121 gatgactcct ggtctagctt ttttttacgg cggattagca cggaagaata atatactaac
      181 gatcatgtct caatcttttc tctccgttgg gataacaacg attatgtggg tatttggtgg
      241 attcggattg gcctttggac gggatatcgg cggtgttatt ggaaacccgg cagatttctt
      301 tttgatgaga catgttacaa gcttccccaa catgtttcat ggtgcaacga ttccattttt
      361 aatgttcttt ctttaccaac tgatgtttat cgtgattacc gttcctttga tgacaggagc
      421 ttttgcaggg agattgaatt taaaaggcta tattatactt gttatttttt ggaatctgct
      481 gatttatttc cctgtttgcc actggatatg gggtggcgga tttttggatc agatgggatt
      541 tagagatttc gctggtggag cagtcatcca cacgacagct ggattcggct ctttagcatg
      601 tatcctcaca cttggtcaaa gaaagattcc tttaggagaa agaagtcagc atagcagtct
      661 tatggcagct gctatcggta caggactgct ttggtttggc tggttcggtt ttaattccgg
      721 tggtgcactt cgagcagatg aacaagcagt caatgcattt ggttcaacat tcatcgctct
      781 tgcatttgcg atgattactt ggctgatcat tgcaaaagta aaaggaaatg gctttgattt
      841 tgtcgacgta ctaacaggat ccgttgctgg attggcagcc attacgcctt gtgccggtta
      901 tgtagaagca aaaagtgcca tgctgattgg gatcattgcg ggtatcgtct gtcatgctgc
      961 tgttgatttt cgtaaaaaga aacaatggga tgatgcactt gatgtgtggg gtgttcatgg
     1021 catgggcgga ttcacaggta cgattctgat agggatcttt gcatctaaca gccatttact
     1081 aacgaatcca agcagctggt acttcttagg gattcaagtg attggtgtca tcattactgc
     1141 cgtgtatgcc tatgtgctga caacagtgat tttaaaatcg gcgatgcact ttacaacgat
     1201 cactactaca aaagaagaac aagaagaagg attggacttt ttcttctttg gcaacaaact
     1261 caattgaaac aaattcaaat agaaaagagg aagattcatg caacaaagca aaacgaaaat
     1321 tcctgcaaaa aaattaacgt tctttggctt tttggctatg accatctcaa tggtcgtttc
     1381 attatatgaa tatcccacat tcgcgacctc aggcttcagc ttagtatttt tcttgttatt
     1441 aggcggtttg ctatggttta tccctgtggc attgtgcgca gcagagatgg caacggtgaa
     1501 aggatgggaa aaaggtgttt cccaaacaaa aagatattaa gaaacagccc atgtttacct
     1561 gcttgcaaac aaaagaatat tgctactaga ctaagagata ctacataagt gtgttggtat
     1621 ctcttttttg ttgccgtgaa acagcagact atttcatttc tgcttgatta ccttaaaact
     1681 tattagtcta tggtttgtca agggcttgtt gcgtgagaaa tgctgaatat caccctttac
     1741 aaaacatagg ctgataagta acttttcaaa agcagaaaag aaatggcaat gcttataaca
     1801 atatgatata atgcagatga agacaaaaaa ggaatttgtc gagctggtta gttcgagata
     1861 attttgagtt tgttaagatg tggtgcactt gatggaatca caggtacgac atcatattat
     1921 ggaggatgaa atagatgata ggtttgattg ttgcgaggtc aaagaataat gttataggca
     1981 agaatggtaa tataccatgg aaaataaagg gagaacaaaa gcaatttaga gagttaacaa
     2041 cgggtaatgt ggttattatg gggcgaaagt cttatgaaga aatcggtcat ccgttgccta
     2101 atagaatgaa tattgttgtt tccaccacaa cagagtatca aggagataat ttagtttcag
     2161 ttaaatcatt agaagatgca ttattattgg ctaaaggacg agatgtatac atatctggtg
     2221 gatatggact atttaaggaa gctttgcaaa tagtagataa aatgtatatc acagaagtag
     2281 atttaaatat tgaagatgga gatacattct ttccagaatt tgatatcaat gattttgaag
     2341 ttttgatagg ggaaacactt ggtgaggaag tgaaatatac gagaacattt tatgtaagga
     2401 aaaaatgaat tgagtaaatt ttggatttag ggtgtt
//
LOCUS       DAGCXG010000298         1504 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.217, whole genome shotgun sequence.
ACCESSION   DAGCXG010000298 DAGCXG010000000
VERSION     DAGCXG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.217"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            262..345
                     /locus_tag="MB022_003014"
                     /pseudo
     CDS             262..345
                     /locus_tag="MB022_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="MB022_003015"
     CDS             394..477
                     /locus_tag="MB022_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBT4467921.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="MB022_003016"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="MB022_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBT4467922.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAGCXG010000299         1351 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.232, whole genome shotgun sequence.
ACCESSION   DAGCXG010000299 DAGCXG010000000
VERSION     DAGCXG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.232"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            48..299
                     /locus_tag="MB022_003017"
                     /pseudo
     CDS             48..299
                     /locus_tag="MB022_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            392..1186
                     /locus_tag="MB022_003018"
                     /note="aph(3')-IIIa"
     CDS             392..1186
                     /locus_tag="MB022_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBT4467923.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          323 g          369 t
ORIGIN      
        1 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
       61 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      121 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      181 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      241 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      301 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      361 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      421 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      481 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      541 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      601 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      661 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      721 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      781 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      841 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      901 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
      961 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1021 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1081 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1141 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1201 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1261 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1321 agcgcctacg aggaatttgt atcgataaga a
//
LOCUS       DAGCXG010000300         1261 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M93370
            SAMN25126038-rid13883063.guided.238, whole genome shotgun sequence.
ACCESSION   DAGCXG010000300 DAGCXG010000000
VERSION     DAGCXG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126038
            Sequence Read Archive: SRR17671401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242188.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:35:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 30 of 67
            Pseudo Genes (incomplete)         :: 36 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 11 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126038-rid13883063.guided.238"
                     /strain="M93370"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-03"
                     /collected_by="Pathology Queensland"
     gene            <1..173
                     /locus_tag="MB022_003019"
     CDS             <1..173
                     /locus_tag="MB022_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4467924.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
     gene            230..622
                     /locus_tag="MB022_003020"
     CDS             230..622
                     /locus_tag="MB022_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4467925.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            623..>1261
                     /gene="aac(6')-Ie"
                     /locus_tag="MB022_003021"
     CDS             623..>1261
                     /gene="aac(6')-Ie"
                     /locus_tag="MB022_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HBT4467926.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYD"
BASE COUNT          500 a          120 c          226 g          415 t
ORIGIN      
        1 taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga gaaaagatta
       61 ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt cttatggacc
      121 tacatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag tagaaatggt
      181 aaaaacattg tatagcattt tacacaggag tctggacttg actgagttta tggaagaagt
      241 tttaattgat gataatatgg tttttgatat tgataattta aaaggatttc ttaatgatac
      301 cagttcattt gggtttatag ctaaagaaaa taataaaatt ataggatttg catattgcta
      361 tacactttta agacctgatg gaaaaacaat gttttattta cactcaatag gaatgttacc
      421 taactatcaa gacaaaggtt atggttcaaa attattatct tttattaagg aatattctaa
      481 agagattggt tgttctgaaa tgtttttaat aactgataaa ggtaatccta gagcttgcca
      541 tgtatatgaa aaattaggtg gtaaaaatga ttataaagat gaaatagtat atgtatatga
      601 ttatgaaaaa ggtgataaat aaatgaatat agttgaaaat gaaatatgta taagaacttt
      661 aatagatgat gattttcctt tgatgttaaa atggttaact gatgaaagag tattagaatt
      721 ttatggtggt agagataaaa aatatacatt agaatcatta aaaaaacatt atacagagcc
      781 ttgggaagat gaagttttta gagtaattat tgaatataac aatgttccta ttggatatgg
      841 acaaatatat aaaatgtatg atgagttata tactgattat cattatccaa aaactgatga
      901 gatagtctat ggtatggatc aatttatagg agagccaaat tattggagta aaggaattgg
      961 tacaagatat attaaattga tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt
     1021 tattttagac cctcataaaa ataatccaag agcaataagg gcataccaaa aatctggttt
     1081 tagaattatt gaagatttgc cagaacatga attacacgag ggcaaaaaag aagattgtta
     1141 tttaatggaa tatagatatg atgataatgc cacaaatgtt aaggcaatga aatatttaat
     1201 tgagcattac tttgataatt tcaaagtaga tagtattgaa ataatcggta gtggttatga
     1261 t
//
